LOCUS       DAOTMX010000001        25087 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_2066_length_25087_cov_3.709931, whole genome shotgun sequence.
ACCESSION   DAOTMX010000001 DAOTMX010000000
VERSION     DAOTMX010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 25087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25087
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2066_length_25087_cov_3.709931"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /gene="lpxD"
                     /locus_tag="P5573_00005"
     CDS             <1..662
                     /gene="lpxD"
                     /locus_tag="P5573_00005"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HRT26479.1"
                     /translation="ESRVVLHPGVVIGAGAVVGEESILYPNVTVYRRCRVGKRVILHA
                     GVVVGSDGFGYADPGRENIKVPQIGIVQIDDDVEVGANSTIDRGTLGRTWIQRGVKID
                     NLVQVAHNVVVGENSVIVSQVGISGSTKLGKSVVVGGQAGLVGHITVGDRVMIAARSG
                     VTKDVAPNSVIAGAPGAPHSRWLKIQGAVARLPEMRSAVKGMLKRIEKLEERIKAREF
                     K"
     gene            659..1432
                     /gene="lpxA"
                     /locus_tag="P5573_00010"
     CDS             659..1432
                     /gene="lpxA"
                     /locus_tag="P5573_00010"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HRT26480.1"
                     /translation="MNIHPTAVVSPEAQLGEGVQIGPYAIIGPDVSIGSNTFIGPHAV
                     IERHTDIGDNCRILQFASIGGDPQDLKYRGEDTRVVIGNNNVIREFVTINRATSADIG
                     VTKIGDNNLLMAYCHVAHNCKLGNFVVMANSANLAGHIHIDDYAIIGGLTGIHQFTRI
                     GRHAFVGGASAIARDVPPFTTVAGNHAKPYGLNIVGLRRRGFDEETLKALKQAYKLVY
                     RSSLLLADALRRIRAEVPDIPEVRQFVEFIEKSERGICR"
     gene            1479..2471
                     /locus_tag="P5573_00015"
     CDS             1479..2471
                     /locus_tag="P5573_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRT26481.1"
                     /translation="MKRDGVVRVGVVGIGRLGNYHLQKYSMMPECVIVGVADIVEERA
                     SKAADTYRCEAFTDHRELIGKVDAVSVTVPTKDHFVVARDFLAAGADVLLEKPITTTL
                     EEADELIATAEQKKAVFQVGFVERFNPAIVALEKVIGRPLFIETHRLHPFFTRGTDVD
                     VVLDLMIHDLDIILHFVRTPVENVDAVGVSILSDKVDIANARVTFHDGCVANITASRV
                     TNKNMQKIRFFGFEGYHSIDYGKREIVSLGARSGANGRLEIGVNDVEVKMQDPLEEEI
                     RSFLRCVITRTAPPVSGREARNSLELALRITEKINSGRERILHAADGRLAETVS"
     gene            2443..3528
                     /locus_tag="P5573_00020"
     CDS             2443..3528
                     /locus_tag="P5573_00020"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRT26482.1"
                     /translation="MVDLQRQYLDLKAEVDEAIGQVLKEARFILGPNVARLEEEIAAY
                     LGSSSAIGVASGTDALLLALRACGVGEGDEVITTPFTFIATAEVIALLGAVPVFADIA
                     PDTFNIDPDRIEEKVTARTKAIIPVHLFGLPADMDGILGIAGRHGLAVVEDCAQAFGA
                     EYKGKKVGTFGNYGCFSFFPSKNLACYGDGGIVVTGNEDSAGTIRLLRNHGSRKRYYH
                     EELGYNSRLDEIQAAVLRVKLKRIDEFNAARRKNADLYRKYIRNASVALPSETADGRH
                     VYHQFTVLSKKRDRIAAALNENEIASAVYYPVPLHLQEVFAHLGYRPGDFPNSERCAA
                     EAISLPMFPELTEEEIRRVCDVINHVS"
     gene            3518..4699
                     /gene="lpxB"
                     /locus_tag="P5573_00025"
     CDS             3518..4699
                     /gene="lpxB"
                     /locus_tag="P5573_00025"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HRT26483.1"
                     /translation="MSPERENRAVQPGRTKKVLIVAGEASGDLHGANLVREMKEVDPG
                     IQFYGIGGKRLQEAGVDLVARSSEMAVVGLTEVFAKFGFILNVFFRLKKSLGAEKPDL
                     AVLIDYPDFNIPLARAAKKSGVKVFYYISPQVWAWRKGRIRSIKKYIDRMAVIFPFEK
                     DFYEKAGVAVTFVGHPLLDTARATGSREEVRLKLGLDRRATTVALLPGSRDNEVTRLL
                     PLMTKAAEILSEEFPGVQFVLPLADTLDEGLVTSIIRRSTAKVLVVADSIYDVLAAAD
                     VAVVTSGTATVETALMETPMVVVYRVSGLTYYVGRLLVDVQNIAMANIIAGKTVVVEL
                     IQDDATAVAIAKEVAEILRDDIKKGAIVEELKKVRERLGSPGAAKRAARLAVELLNSN
                     G"
     gene            4712..6484
                     /gene="msbA"
                     /locus_tag="P5573_00030"
     CDS             4712..6484
                     /gene="msbA"
                     /locus_tag="P5573_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HRT26484.1"
                     /translation="MELYKRLLKLARPHWLRFIFAMLCMAVVGATTGAAAFLVKPAMD
                     DIFLNRDAAALKWIPVAVVVIYLLKGLGNYGQTIIMSYIGQRVVTDLRNQLYNHILRQ
                     PLSFFTKYPTGNLMARLTSDVGLVQGAVSEAVTSILKDSFSLIGLIFVIFYRDWKLAI
                     VASFIFPLAIYPVLSFGRKMRKISGYTQVTVGSLTSLLQETISGNRIVKAFGMESYEN
                     KRFAKENERLFKLTMKSVSVRAVSSPLMEFFGGIGIAAIIIYGGYQVLHGISTPGTFF
                     SFLAALMMLYEPAKRLTNVNNTIQQGLAGAERIFSVIDTVPEIKNKEGAVELPRISRE
                     ICFDDVSFRYEETLVLKHINLRIKAGECIALVGMSGGGKTTLVNLIPRFYDVTEGRIL
                     IDGYDIRDVTIESLRAQVGIVTQQTILFNDTVRNNIAYGDIRKSDEDIVKAAKAANAH
                     DFIMKLPQGYDTIIGEQGVKLSGGERQRISIARALLKDAPILILDEATSSLDTEAEIE
                     VQEALDKLMEGRTTFVIAHRLSTIRNADRIVVLVDGRIVEEGTHESLLRRRGEYYKLY
                     SMQFRDNAINGVEAQHLTDAQCLP"
     gene            6493..7629
                     /gene="lpxK"
                     /locus_tag="P5573_00035"
     CDS             6493..7629
                     /gene="lpxK"
                     /locus_tag="P5573_00035"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HRT26485.1"
                     /translation="MTKAWDFDGQSDFSNPFHSFLYTLSLAYGGAVRLRNVLYDGGLL
                     GVKRLPRPVVSVGNITAGGTGKTPMVIFLTKLLRERGYRPAVLSRGYRGKGEGRVNVV
                     SDGDHVTASPAEAGDEPFLIASSTGAAVLTGADRFATGSHAVERLGADILVLDDAFQH
                     RRLFRDVDILLLDADRPFGNRCLLPRGPLREPVNGLRRADMIVLTRSGEDSGESPVEE
                     ELQAKYPGIPVFRAVRQPKEIVPLSAGSFPPIQADPLESSPSSPQKREHDSPPESLQG
                     RRVCAFAGIANPDSFRKTLASLGAETVAFLTFPDHHRYRVEDIEAVRKAAEKGKCEIT
                     LTTEKDGVKLRSFPTAGIHALRVEMSVTGGAGEFVEAVLEKLGR"
     gene            7626..8504
                     /locus_tag="P5573_00040"
     CDS             7626..8504
                     /locus_tag="P5573_00040"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRT26486.1"
                     /translation="MKPTKATRRIMFFFRLIPLGLRKKIFTAALLLFYHMSPKQRLIA
                     LHNLSRAYPEKDMAEIVKIAKGVFRHVGIVIAEFFELPFITKETLHQWVEFDGLENLQ
                     KAIAQNQGVLAVGSHFGNWELMTIALPMAAKPMHIIHRPLDNPIIEDMLAWVRTLHGN
                     SLIPKEGAAKKILRLLRKSEIAGILSDQNMAAREGVFVDFFGRPACTAVGTAVLAMRT
                     EAPVLPAFMPRMPNGKYKFVIKPPVKITVTGDDQADILVNTQRFTKVVEDMVRQYPDQ
                     WFWVHQRWKTKPCQIE"
     gene            8489..9580
                     /gene="waaF"
                     /locus_tag="P5573_00045"
     CDS             8489..9580
                     /gene="waaF"
                     /locus_tag="P5573_00045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HRT26487.1"
                     /translation="MPDRVEVEMLKVRGRAPFPFGRVRNILVRSTNWIGDVVMTFPAI
                     AAIRKTLPEARITVLAKPWVAELLCLCPDVDEVMLFQSPGVHDGLSGKLRLAAELKAR
                     QFDAAILLQNAVEAAIIARLAGIPVRAGYDSDCRGLLLTHSVGRTREIRRVHQVDYYL
                     EMVKALGFASAGRDVRLGLGDDYRKAGDDFLRHYGVDTGRFLVGLAPGATYGPAKRWY
                     PDRFARVADRLREEFNGETLLFGSKADHAVAEEVKKHAAYPVVNVAGKTSLKEAIALI
                     ARCRLFISNDSGLMHVAGALGIPLIAVFGSTNPVTTSPVGEKSLIVTKRVSCSPCLKE
                     KCDTDFRCMDLITADEVYEAAKGLLASVL"
     gene            9653..10243
                     /locus_tag="P5573_00050"
     CDS             9653..10243
                     /locus_tag="P5573_00050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRT26488.1"
                     /translation="MKNNVAVFMDRDGTINEEVGYLGRVEELVLIPVAAEAVRLVNEE
                     GMKAVVVTNQSGVARGFFTEEDVRLINGRLAEMLRDEGAVIDRFYYCPHHPTEGREPY
                     RVACACRKPEPGMLLQAAAELDIDLARSYLIGDMLKDLDAAHRAGAKGVLVRTGYGKN
                     VGVTDGPDYIAEDILDAVRWILDDRKKARHPSPGPR"
     gene            10262..11299
                     /locus_tag="P5573_00055"
     CDS             10262..11299
                     /locus_tag="P5573_00055"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HRT26489.1"
                     /translation="MNILIVKLSAVGDVVHTLPSLAALRKLYPDAHITWVIEEAAADL
                     VTGHPSLDRVLVSRRKSWAKSLGSPRRALRAVDEVRSFLRTLRDRPYDLVIDFHGLFK
                     SAVIVFLSRGKRKLGYDSLQELSGLFLNEKIPEDMDKHAVDRYLDFLRHLGAPEEEPA
                     FLIPVGEENRRRVKGFLEEYGLDRGAGFVAVSPVALWETKLWEENRFAALCDRVVGEL
                     GLKVVLTGDKAGGVLGRIEARMTESAVNLGGKTTLRDLAALYKRARVLVTTDSGPMHI
                     AAAVGTPVIALFGPTAPWRTGPYGPGHIVIRKAIACSPCFLKHCATGQCMKDITVEEV
                     FEAVKKGIADV"
     gene            11322..11936
                     /locus_tag="P5573_00060"
     CDS             11322..11936
                     /locus_tag="P5573_00060"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HRT26490.1"
                     /translation="MKPRIGFTLSFFVVVFLNLIVFIPFVFAEDRVSTAAANPETRFW
                     KESCVVIGYGTSEIHEGHYEPMLFIWHLAYDLRNYIPALKNHKGILAGVCEPQVNPAF
                     RPSSDFEFGVGVGLKYLYPVTDRLYPYIQLTVGPHYISLQTEDQASGFIFSNTVGVGI
                     YYFVTKNSAAMVGYRARHISNAGTREPNDGINTHFGTIGYSFFF"
     gene            11959..12129
                     /locus_tag="P5573_00065"
     CDS             11959..12129
                     /locus_tag="P5573_00065"
                     /inference="COORDINATES: protein motif:HMM:NF036846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HRT26491.1"
                     /translation="MVISKELLDILACPNCKGDIRLNEAGDGLVCDHCKLLYEIKDGI
                     PIMLVEEAKKIE"
     gene            complement(12131..13045)
                     /locus_tag="P5573_00070"
     CDS             complement(12131..13045)
                     /locus_tag="P5573_00070"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRT26492.1"
                     /translation="MDLSVVIVNWNTKDLLRGCLQSVYRTLKDIHFEVIVVDNASQDG
                     SVAMLKEDFPDVKIIENETNRGFGAANNQALEVMQGRYALLLNTDTVLTDGAVRQLFF
                     FLEQHPDTAMACGQLLNEDGSRQNSIAAFPSLLTLLFNTSILEYLFPKRYPSKRKDYK
                     EPVEVESCIGACLMVRKKAIDEVGLFDERFFFFLEETDWAFRMKKAGWKVYFIPSAAI
                     YHFQGRSIGHGVESRIAFYRSRYAYFRKWYKRPQYILARLLILCRLTVDWVSMAAANL
                     FLLGANRRIRTKLSVYSRLILWHLRGCP"
     gene            complement(13046..13816)
                     /locus_tag="P5573_00075"
     CDS             complement(13046..13816)
                     /locus_tag="P5573_00075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRT26493.1"
                     /translation="MTDKIPLSVAIITKDEAENLPDCLRSVSFAGQIVVVDSGSTDGT
                     VQLASDLGCEVFEVPWRGFGPQKQSAVDRCRHPWILVLDADERIPAETAEAIKGIVTG
                     QDKGAAGYSFPRKNYFQGRWVRHMGWWPDRIVRLFRRGVGRMSEATVHESVQVDGPVA
                     ALDVPIEHFTEGRFSKILMKIDRYSTLGAQEAFAEGRRASIGEAVLRAELAFLQNYIL
                     RLGFLDGRQGFILSVTDSVNKFFKYAKLSELSRGAKKV"
     gene            14156..15346
                     /locus_tag="P5573_00080"
     CDS             14156..15346
                     /locus_tag="P5573_00080"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRT26494.1"
                     /translation="MAEYSVPVLLAVYIFAQPFHYLTAVEEVGIYLAFLISVYLVLTK
                     KVEFTFDTPLSLPFVLFALWALIGIAFAINKPNSIHDFYAHLLKYYVLFFLVFNFFRT
                     RDKFLALALIITVSFTIFSLWGMVCVYGVEGKKLFVDRFTSPAFLPYRTYIHVFGAIT
                     AMSFFYSETAVSRKSFFALCIFVDVLATFLSQTRSAIIALTAALYLLLIVKRKYKQIV
                     LITVLLLAVVYFSPVKDRFLDEREYTVRIANYYLYSAIIRDYPIKGIGFGMQTYQDPG
                     LNIDSYNKYVPEKIRVSPPNLIPHNTFFDIAVRLGLIGLGLYVLIIFMSLKMLFVVIR
                     NRADPFLREWGFCLCANFLAFFIQAMFTETSFGVQALTQYLLFAMIAILWKIDKEGVG
                     TVGE"
     gene            15542..16111
                     /locus_tag="P5573_00085"
     CDS             15542..16111
                     /locus_tag="P5573_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRT26495.1"
                     /translation="MIRDLVLKNRSYRRFYEDFSVDRETLQELVDLARLTPSAANKQP
                     LKYILSCDREKNAVIFPNLAWAGYLKDWPGPSEGERPSAYIIVLVDTEISRSADIDCG
                     IAAQTILLGAVSKGLGGCMIASVRRDSLREALKIPARYEIILVIALGKPKETVIIEKM
                     GSDGDVKYWRDGEGRHHVPKRSLEEIILP"
     gene            complement(16150..17166)
                     /gene="gap"
                     /locus_tag="P5573_00090"
     CDS             complement(16150..17166)
                     /gene="gap"
                     /locus_tag="P5573_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HRT26496.1"
                     /translation="MVRIGINGFGRIGRQVLKAIMERQAATLQVVAVNDLFDTKTNAH
                     LLKYDTNYGRFGGTVVAERDRFLINDQPVQSFACRDPGEIPWDEFGVDIVVESTGLFT
                     SAERAAVHRAAGVKKVIITAPAKGEDLTVVMGVNEDAYDPLKHHILSNASCTTNCLAP
                     PVSVIHQAFGVEQGMMVTVHSYTNDQRILDLPHKDLRRARAAAQNIIPTTTGAARAIA
                     LVIPDLAGRFDGYALRVPTPTVSVVDFSAQLKKGATTEELRAVLKEACEGRLKGIMAC
                     EEEPLVSTDFKGDEHSSTVDVEFTQVLRGKGNLAKCVAWYDNEWGYSCRVADLAAYIA
                     ARGL"
     gene            complement(17227..>17835)
                     /locus_tag="P5573_00095"
     CDS             complement(17227..>17835)
                     /locus_tag="P5573_00095"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRT26497.1"
                     /translation="KIVNRYRDTKNRRGKMPEKEMDYFTALYDVAKVINASLEPARVL
                     EEIVRSVAEAMKVKASSLRILDERKKRLNLGAYHGLSSDYVHKGPVLVEESGIDQEAL
                     KGKVIWIEDAQTDPRFQYNDMARAEGIRSVLVVPLVVGTTAVGVLRVYTDDVKRFKEK
                     EIRFMEAAANLSAIALDNARMHQHLRRERDLMAESKYRLDDN"
     assembly_gap    17836..17845
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(18092..18580)
                     /gene="ybaK"
                     /locus_tag="P5573_00100"
     CDS             complement(18092..18580)
                     /gene="ybaK"
                     /locus_tag="P5573_00100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HRT26498.1"
                     /translation="MTKDHFPATPAIRMMKEKGVTFTLHPYKYEERGCTETAARELRV
                     DEYRVVKTIVMEDDERKSPFIVIMHGNRHVSTKNMARFLGVKSVSACDPKVAEKHTGY
                     MVGGTSPFGTRKRLPVYVEATVLDLPKILINAGRKGLLAEMAPTELLRVLDAQTVTVA
                     VR"
     gene            complement(18614..19540)
                     /locus_tag="P5573_00105"
     CDS             complement(18614..19540)
                     /locus_tag="P5573_00105"
                     /inference="COORDINATES: protein motif:HMM:TIGR01212.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HRT26499.1"
                     /translation="MSSLPYNDLARAMRERFGCRVQKVTLDAGFTCPNRDGKVGTGGC
                     IYCNTRGSGTGAFSSLSVREKLERGKAALEKRYKAEKFIAYFQSFSNTYGPVETLRRR
                     YEEALGVPEVVGLAVGTRPDCVDDEVLDLLAELNRRSFVSVEYGLQSAHDRTLELINR
                     GHTFSVFQEAVDRTRRRGVDVCVHVILGLPGEGRREMLETAEALAGMDIQAVKIHLLY
                     VVKGTVLHRLYESGAYRCLDREEYAGIVAEFLSLLPPSVVIHRLTGDPHPAELVAPQW
                     ALEKQANLRAIREAMEERNTWQGKSWKPGMSF"
     gene            19728..21602
                     /gene="mnmG"
                     /locus_tag="P5573_00110"
     CDS             19728..21602
                     /gene="mnmG"
                     /locus_tag="P5573_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041585804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HRT26500.1"
                     /translation="MSSQYDVIVVGAGHAGCEAALAAARMGCRTLLFSINLDTVAHMP
                     CSPSIGGVGKGHLVKEVDALGGEMGKAADLTALQYRTLNTRKGPAVRSTRTQNDKARY
                     RILMKGVVERQPLIDLKQTLVEEILAEGGRVVGVRDHIGAEFFAPTVVLTTGTFLHGL
                     VHIGRKQIPSGRAGEFPADGLAESLRNLGFRMGRMKTGTPARVRRDTIDFCRFAELRG
                     DEEPNPFSLFTEKTEITQIPCYIGRTGERTGEIIRRNIHLSPLYNGTIQGVSARYCPS
                     LEDKMMKFPEREHHQIILEPEGLDTEEIYVSGTGNSLPYEVQIDLVRSVPGLEEARIM
                     RPAYAIEYDFVYPTQLKPTLETKLVEGLFFAGQINGTSGYEEAAAQGLWAGINAALKV
                     QKRRPFILDRSEAYMGVMVDDLVTRGTREPYRMFTSRAEYRLLLREDNAAFRLVEKGF
                     ELGLQDREALERVVEKERRVELETRRLEQKRVRSTPEANRILEKAGSVPIDESATLSQ
                     ILKRPEVSYADVEAIEGVISDLPADVKRQVEVRCKYDGYIRRQEAEVKKFKHLERLEI
                     PGDFDYRGIPGLSNELKQKLGEIRPLSLGQASRIEGMTPAALSVLMVFLKRHREGKAS
                     "
     gene            complement(21664..22677)
                     /locus_tag="P5573_00115"
     CDS             complement(21664..22677)
                     /locus_tag="P5573_00115"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HRT26501.1"
                     /translation="MDRRLSRTKKTATVVLALVGIGTMAFYTLCDGECSYLQGDVLGI
                     DLKLVGILFMAVVAVLAWAERSHFLRAALAAGMGGEVFLLWFQLKEGVYCPYCLAFAA
                     CVVLAFAVNYEPPAERGWRRLLYLLGGVRFSPASRHYPLILFVLAGFAFFVLTFSGSA
                     LPAYAAETGPYVYGQGEREVRVYSDYFCAPCRRVEPKIEKLTEEIARDGKGRVLFIDT
                     PMHRETVLYARYYIYATGAGGETLESANQVRRALFEAAEKGLKTESELRNFLEAKKIK
                     VVQRDTAPFFRKYNDYIKEDGVRSTPTVVIAGRAGKKHYSGEDDVLRALEELRLDGGS
                     GAE"
     gene            complement(22687..23283)
                     /gene="pyrE"
                     /locus_tag="P5573_00120"
     CDS             complement(22687..23283)
                     /gene="pyrE"
                     /locus_tag="P5573_00120"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HRT26502.1"
                     /translation="MTNKDLEKMKDRLADLIIERSFRYNEDNPFTLSSGRKSPYYFDC
                     KPTTLDPEGMYLIGNLVFDILKDTDVKAAGGLTLGADPIANALALISFEKGRPIRSFI
                     VRKDAKAHGTRSKIEGNVRPGDKVAVIDDVVTTGASTITAVESAREAGLTVELAVVLI
                     DREEGGRELIERHVRPVVPILTRSEIMARYREKAAGKS"
     gene            complement(23280..>25087)
                     /locus_tag="P5573_00125"
     CDS             complement(23280..>25087)
                     /locus_tag="P5573_00125"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HRT26503.1"
                     /translation="GCNCSTGPEDMMRFISAMKPHATVPLAAKPNAGIPVVKEGETVF
                     PMDASEFGAFGKELASAGAALVGGCCGTTPDHIRSLKRNLKNKKPILPAKRSIAALTS
                     PRKTVFLDRGGALKVIGERINPTGKKALREALREEFTEGRFTLVRQIARDQEREGADL
                     LDVNVGVPGIDEVKAMTGAVDVLAVATALPLVIDSPKVETIEAALRRYPGRALLNSIS
                     GEEEKIDRLLAAAARYGAMFILLPVAKGEVPRTAARRKEVIRDVFRRARARGFTKEDL
                     VVDGLVMTVAADPRAALETLETVEWCSRRFGTRTVLGLSNVSFGMPGRMWINAAFLAM
                     AAGKGLTLAIANPGQAELMNIKAASDLLTQKDGDAAAFIARFSEQAANSGKRETPETT
                     TPEEKVYQAILEGNREDIGRLLAAALAAGASPLGLVEDVMIPAITEVGGRFDRREYFL
                     PQLIASAEAMKQGMDYLEPHLKEDLAADDRKTVILATVEGDIHDIGKNIVALMVKNHG
                     YRVIDLGKDVPAGKIIEAAKAHRGAVVGLSALMTTTMVRMKEVMELAAKECSDCRFLL
                     GGAVVTKAYARSLGAEYAKDGVEAVRKLEEIFGKT"
BASE COUNT         5181 a         7342 c         7386 g         5168 t
ORIGIN      
        1 ttgaaagcag ggtcgtcctg cacccgggag tagtcatagg cgcgggggcc gtggtcggtg
       61 aggaaagcat cctttacccc aatgtgacgg tctaccgccg ctgccgggta ggcaagcggg
      121 taatccttca tgcgggtgtt gtggtgggca gtgacggctt cggctacgcc gaccccggac
      181 gggagaacat caaggtgccc caaatcggca tcgttcagat agacgacgac gttgaggtgg
      241 gggccaattc gacgatagac cggggaaccc tgggcaggac ctggatccag agaggagtca
      301 agatagacaa cctcgtccag gtcgcccaca acgtcgtcgt cggggaaaat tcggtcatcg
      361 tttcccaggt cggtatttca ggcagcacaa agttgggtaa gagcgtagtt gtcggcggtc
      421 aggcgggcct cgtgggccac atcaccgtgg gggaccgcgt gatgatcgcg gcaaggtcgg
      481 gcgtcacaaa ggacgtcgca ccgaacagcg tcatcgcggg ggcacccggc gctccacata
      541 gcaggtggct gaaaattcag ggcgccgtcg cccgattgcc cgagatgcgg agcgcggtca
      601 agggcatgct gaaaagaatc gaaaaactcg aagaaaggat aaaagcaagg gagtttaaat
      661 gaacatccat ccaacggccg ttgtatctcc cgaagcacaa ctgggggagg gcgttcaaat
      721 cggcccttac gcaatcatcg gtcccgacgt aagcatcggg tcgaatacgt tcatcggtcc
      781 gcacgccgtc atcgaaaggc atacggatat aggtgataac tgtcgtatcc ttcagttcgc
      841 ctccatcgga ggcgaccccc aggacctcaa gtatagaggc gaagacacga gggtcgtcat
      901 cgggaataac aatgtgatcc gcgagttcgt gaccatcaac cgcgctactt cggcggacat
      961 cggtgtgacg aagatcggcg acaacaacct cctcatggct tactgccatg tggctcacaa
     1021 ctgcaagttg ggcaacttcg tcgtgatggc caattcggcg aacctggccg gtcacatcca
     1081 catcgatgat tatgccatca tcggcggtct gacggggatc caccaattca cacgcatcgg
     1141 cagacacgcc ttcgtcgggg gtgcttcggc catcgcccgc gatgtgccgc ctttcacgac
     1201 cgtcgccgga aaccacgcga aaccctacgg cctgaatatc gtaggactcc ggcgaagggg
     1261 ttttgacgaa gagacgctca aggcgttgaa gcaggcctac aaactcgttt accgctcatc
     1321 cctcctcctc gccgacgccc tccggaggat acgggctgag gtgccagaca tcccggaggt
     1381 aaggcagttc gtcgaattta tcgagaagtc ggagcgggga atatgcagat agggcgggcg
     1441 aaaggtcagg gacggaaggc atagggtaaa gaaaaaacat gaagcgggac ggagtcgtca
     1501 gggtcggagt ggtcggaata gggcgtctgg gcaactacca cctccagaag tacagtatga
     1561 tgcccgaatg cgtcatcgtc ggcgtcgccg acatcgtcga ggagcgtgcc tcgaaggcgg
     1621 ccgacactta ccggtgcgag gcattcacag atcacaggga acttatcggc aaggtggatg
     1681 cagtgagcgt caccgtcccc acgaaggacc atttcgtcgt cgccagggac ttcctggctg
     1741 ccggggcgga cgtcctcctg gagaagccga tcacgacaac gctcgaagag gcggacgaac
     1801 tgatcgctac cgccgagcag aagaaggccg tctttcaagt cggttttgtc gaaaggttca
     1861 accccgccat cgtcgccctc gaaaaggtca tcggccggcc cctttttatc gagacgcacc
     1921 gcctccatcc atttttcacc cgcgggacag acgtggacgt agtccttgac ctgatgatcc
     1981 atgacctcga cataatcctc cacttcgtga ggaccccagt agaaaacgtg gacgccgtcg
     2041 gcgtatccat cctttccgac aaggtcgaca tcgccaacgc ccgggtgacg tttcatgacg
     2101 gttgcgtggc caacatcaca gcgagcagag tcaccaacaa gaatatgcag aagatacgat
     2161 ttttcggttt cgagggttat cactccatcg actacggaaa acgggagatc gtctccctcg
     2221 gcgcgaggag cggtgcgaac ggcaggctgg agatcggcgt caacgacgtc gaggtgaaga
     2281 tgcaggaccc gttggaagaa gagatccggt ctttcctccg ttgtgtcatc acccggactg
     2341 cgcccccggt atccgggaga gaggcgagga attcgttgga gcttgcgctc cgcatcacgg
     2401 aaaaaataaa ctcgggaagg gaaagaatcc tccatgctgc cgatggtaga cttgcagaga
     2461 cagtatcttg acctcaaggc tgaggtcgat gaggccatcg ggcaggtctt gaaagaggcg
     2521 cggttcatcc ttggcccgaa cgtcgcaagg ctggaagaag agatcgccgc ttatctcggg
     2581 tcgtcttccg ccatcggcgt cgccagcggt acggacgccc tgctccttgc cctccgggcg
     2641 tgcggggtcg gcgagggcga cgaggtgatc acgacgccct tcaccttcat cgccaccgcg
     2701 gaggtcatcg ccctgttggg ggccgttccc gtcttcgcgg acatcgcgcc ggacaccttc
     2761 aacatcgacc ccgacaggat agaggagaag gtcaccgccc ggacgaaggc gatcattccc
     2821 gtccatcttt tcggcctccc ggcagatatg gacggcatcc tcggcatagc cggtagacac
     2881 ggcctggcgg tcgtcgaaga ctgcgcccag gccttcggcg ccgaatacaa gggaaagaag
     2941 gtcgggacct tcggcaacta cggctgcttc agtttcttcc ccagcaagaa cctcgcttgt
     3001 tacggcgatg gaggaatagt cgtgacagga aacgaggatt cggccggcac gataagactc
     3061 ctgagaaacc atgggagcag aaagaggtac taccacgagg aactcggcta caacagccgc
     3121 ctcgacgaga tacaggcggc tgtcctgcgt gtaaagttga agaggatcga cgaattcaac
     3181 gccgcccgga gaaaaaacgc agacctgtac cgcaagtaca tacgaaacgc cagtgtggcg
     3241 cttccctcag aaacggccga cggcaggcac gtctaccacc agttcacggt tctgtcgaaa
     3301 aagcgcgacc ggatagcggc tgccttgaac gaaaatgaaa tagcgtcggc ggtttactac
     3361 cccgtccccc tccatctcca ggaagtcttt gcccacctcg gttacagacc cggggatttc
     3421 cccaacagcg aacgttgcgc cgcggaagcg atctcccttc ccatgttccc ggaattgacg
     3481 gaagaagaaa tccgacgcgt ctgcgacgtc ataaaccatg tctcctgaaa gagaaaaccg
     3541 tgccgtgcag ccgggacgca caaaaaaggt cctgatcgtc gccggggaag cgtcgggtga
     3601 cctccacggg gccaacctgg tgcgggagat gaaagaggtc gaccccggca tccagtttta
     3661 cggaatcggg gggaagaggc tgcaagaggc cggcgttgat ctggtcgccc gttcgtcgga
     3721 gatggccgtc gtcggcttga cggaggtctt cgcgaagttc ggcttcatcc tgaacgtctt
     3781 ctttcgcctg aagaaatcgc tcggcgcaga gaaacccgac ctcgccgtcc tcatcgatta
     3841 tccggacttc aacatccctc ttgccagggc cgcgaagaag agcggtgtca aagttttcta
     3901 ctacatcagc ccccaggtct gggcctggag aaaagggcgt atccggagca taaaaaagta
     3961 catcgaccgc atggcggtga tctttccctt cgagaaggac ttctacgaaa aagcgggcgt
     4021 cgccgtgacc ttcgtcggcc accccttgct cgacacggcc cgggcgacgg gttcgcggga
     4081 ggaagtccgt ctgaagctgg ggctcgaccg gcgcgcaacc acagtcgcac tccttcccgg
     4141 aagccgggat aacgaagtca cccgtctgct cccgttgatg acgaaggcgg cggaaatctt
     4201 gagtgaagaa ttcccgggcg tccaattcgt cctccctctc gccgacaccc ttgatgaagg
     4261 tcttgtgacg agtataatac ggcggtccac ggccaaggta ctcgtcgtcg ccgactccat
     4321 ctacgacgta ctcgctgcgg ccgacgtagc cgtcgttacc tccggcaccg cgacggtcga
     4381 gacggccctc atggaaacgc ccatggtggt cgtctaccgg gtctcgggcc tcacttacta
     4441 cgtcggcagg ttgctcgtcg acgtgcagaa catcgccatg gccaacatca tcgccggtaa
     4501 gacggtcgtc gtcgaactga tccaggatga cgcaactgcc gtggcgatag caaaagaagt
     4561 tgccgaaatt cttcgagatg atataaagaa aggtgcgatc gtcgaagaac tgaagaaggt
     4621 aagggaaagg ctcggcagtc ccggcgctgc gaagagggcg gcccgactgg cggtcgagct
     4681 gttgaattcg aacggttgaa acgataaggc tatggaatta tacaagcggc ttttaaaact
     4741 cgcccggcct cactggctgc gttttatctt cgccatgctc tgcatggccg tcgtgggcgc
     4801 gacgacgggt gcggcggcct ttctcgtaaa gcccgccatg gacgacatct tcctcaaccg
     4861 ggacgcagcc gccctcaagt ggatccccgt cgccgtcgtc gtcatctacc tgctgaaggg
     4921 cctcggcaat tacggccaga cgataatcat gagctacata ggccagcgtg tggtcacgga
     4981 cctcaggaac cagctttaca atcacatctt aaggcagccg ctctcgttct tcactaaata
     5041 ccccacgggc aacctgatgg cccgtctcac gagcgacgtg ggtctggtgc agggcgccgt
     5101 atcggaggcc gtaacgagca tcctcaaaga ctccttcagc ctcatcggac tcatcttcgt
     5161 catcttttac cgggactgga agctcgccat cgtcgcttcc ttcatcttcc cgctggcgat
     5221 ctatcctgtg ctgtctttcg gccggaagat gaggaagatc tccggctaca cccaggtaac
     5281 cgtagggagt ctcacttctt tactccagga gacgatctcg ggtaatcgta tcgtcaaggc
     5341 cttcggtatg gagtcttacg agaacaaacg tttcgctaag gaaaacgagc gcctcttcaa
     5401 actgacgatg aagtccgtgt cggtgcgcgc cgtctcgagc ccgctcatgg agtttttcgg
     5461 gggtatcggg atcgccgcga tcatcatcta cgggggatac caggtccttc atggcatctc
     5521 tacccccggc acgttctttt cttttctggc ggcgctcatg atgctctacg aaccggccaa
     5581 gaggttgacg aacgtcaaca acacgatcca acaagggctg gccggcgccg agcggatctt
     5641 cagcgtcatc gacacggtcc ccgaaatcaa aaacaaagaa ggtgccgtag aactaccgag
     5701 gatatcaagg gagatttgct tcgacgacgt ctctttccgg tatgaagaaa cgctcgtgct
     5761 gaagcacatc aacctgagga tcaaggccgg agaatgcata gccctcgtcg gtatgagcgg
     5821 cgggggaaag accacgctcg tcaacctgat tccccgtttc tacgacgtga cggaaggacg
     5881 gatcctgatc gacgggtacg acatccgtga cgtgaccatc gaatccttac gcgcccaggt
     5941 cggcatagtc acccagcaga ccatactttt caacgatacc gtgaggaaca acatcgctta
     6001 cggtgacatc cgaaagtccg acgaagacat agttaaagcc gcgaaggccg ctaatgccca
     6061 cgacttcatc atgaaactgc cccaggggta cgataccatc atcggcgagc agggggtaaa
     6121 gttgtcgggc ggcgaaagac agaggatatc gatcgccagg gcccttctca aggatgcccc
     6181 catactgatc ctggatgaag ccacttcatc cctcgacacc gaggcggaga tcgaggtcca
     6241 ggaggccctc gacaaactca tggaagggcg gaccaccttc gtaatcgccc accggctgtc
     6301 gacgatccgc aatgccgaca ggatcgtcgt cctcgtggac gggcggatcg tcgaagaggg
     6361 cacccatgag tccctgttgc ggcggagagg agagtattac aaattgtaca gtatgcagtt
     6421 cagggataac gcaattaacg gagtggaggc gcaacacctg accgacgccc aatgcctgcc
     6481 gtgaaacgaa tcgtgacgaa agcctgggac ttcgacgggc aatcggattt ctcgaacccc
     6541 ttccactcct tcctctacac cctttctctg gcttacgggg gcgccgtgcg gttgagaaac
     6601 gtcctctacg acggcggact cctgggagtg aagaggctgc cccgtcctgt agtgagcgtc
     6661 gggaacatca cggcgggggg cacggggaaa actccgatgg tcatctttct gacaaaactg
     6721 ctgcgagaga ggggataccg tcccgccgta ctgagtcgtg gttacagggg aaaaggcgaa
     6781 ggacgggtaa acgtcgtctc cgacggcgat cacgtcacag cctcccccgc cgaggccggc
     6841 gacgaaccgt tcctcatcgc ctcatcgacc ggggcggcgg tcctgaccgg agcggaccga
     6901 tttgctacgg gaagccacgc tgtcgaaagg ctgggtgcgg acatcctcgt actcgacgat
     6961 gccttccagc accgaaggct ctttcgggac gtcgacatcc tgctcctcga cgccgacagg
     7021 cctttcggga atcgatgtct cttgccgcgg ggaccgttgc gggaacccgt taatggtctg
     7081 agacgggcgg atatgatcgt cctcacgagg tccggcgaag atagcggaga atcgccggtc
     7141 gaggaggaat tgcaggcgaa gtatccgggg ataccggtct tccgggccgt acgacagccg
     7201 aaggaaatcg tccccctgtc cgccgggtct ttcccgccca tacaggccga cccgctggag
     7261 tcttcccctt cttcacccca aaagagggaa catgattccc cccccgaatc cctccaaggc
     7321 aggcgggtct gcgccttcgc tgggatcgcc aaccccgatt ccttcagaaa aaccctggcc
     7381 tccctgggag ctgaaacggt cgcgttcctt acctttcccg accatcaccg ttacagggtc
     7441 gaagatatcg aagccgtcag gaaggcggcg gagaaaggca agtgcgaaat caccttgaca
     7501 acggaaaaag acggggtaaa actacgcagt tttccgacgg cgggcattca tgccctgagg
     7561 gttgagatgt cggtcacggg cggggcgggc gaattcgtcg aggccgttct tgagaagctg
     7621 gggagatgaa accgacaaag gcaacacggc gtatcatgtt tttcttcagg cttatcccgc
     7681 ttgggttgag gaagaagatc tttacggccg cgctccttct cttctaccac atgtcgccga
     7741 agcagcggct gatcgccctc cacaacctta gccgcgccta cccggaaaag gacatggcgg
     7801 agatagtcaa gatagccaag ggcgttttcc ggcacgtcgg catcgtcata gcggaatttt
     7861 tcgaactccc tttcataacg aaagagaccc tccatcagtg ggtggagttc gacggactcg
     7921 aaaacctgca aaaggcgatc gcccaaaatc aaggtgtcct cgccgttggg tcccatttcg
     7981 gcaactggga actgatgacg attgcgttac ccatggcggc taaaccgatg cacatcatcc
     8041 accgtcccct ggacaatccc ataatcgaag acatgctggc ctgggtcaga accctgcatg
     8101 gaaattcgct aatcccgaag gaaggggccg caaagaaaat cctgagactg ctgcggaaaa
     8161 gcgagatcgc gggcattctc agcgaccaga acatggcagc gcgagagggg gtcttcgtgg
     8221 atttcttcgg ccgcccggcc tgcacagcag tgggcacggc agtcctggcc atgaggacgg
     8281 aggcgccggt cctgccggca ttcatgcccc gtatgccgaa tggaaagtac aagttcgtca
     8341 tcaagccgcc ggttaagatc accgtgaccg gcgacgacca ggccgacatc ttggtcaaca
     8401 cccagcgttt tacgaaggtc gttgaagaca tggtgcggca gtatcccgac cagtggttct
     8461 gggtccacca gcgatggaag actaagccgt gccagataga gtagaagttg agatgctgaa
     8521 ggtcaggggc agggcgcctt tcccgttcgg cagggtgcgg aacatcctgg tgcggagcac
     8581 aaactggatc ggcgatgtcg tcatgacctt cccggcgatc gccgccataa ggaaaaccct
     8641 gcccgaggcc aggattacgg tcctggcaaa gccctgggtg gccgagctcc tctgcctctg
     8701 ccccgacgtg gatgaggtca tgctctttca gagtcccggt gtccatgacg gcctttcggg
     8761 aaaactcagg ctcgccgccg agcttaaggc aaggcaattc gacgcagcga tcctcctgca
     8821 aaatgcggtc gaggcggcga tcatcgcccg cttagccggt ataccggtaa gggcgggtta
     8881 cgattccgac tgcaggggac ttctcctcac ccattccgtg gggcgtaccc gggaaatccg
     8941 cagggtgcac caggtcgatt actacctgga gatggtgaag gctctcggtt ttgcgtcggc
     9001 gggcagagac gtgcggctcg gcctcggtga tgattaccgg aaggccggcg atgacttcct
     9061 ccggcattac ggagtcgaca caggcaggtt cctggtgggg ctggcccccg gcgctaccta
     9121 tggcccggca aagagatggt atcccgacag gttcgcccgg gtagccgatc ggctccgcga
     9181 agaattcaac ggggaaactc tgttgttcgg gagtaaagcg gaccacgccg tggcggaaga
     9241 ggtaaagaaa cacgccgctt acccggtcgt caacgttgcc gggaagacgt cgctcaaaga
     9301 ggcgatcgcg cttatcgcgc ggtgccgcct cttcatctcc aacgactcgg ggttgatgca
     9361 cgtcgccggc gcgctgggca tacccctgat cgccgtgttc ggttccacca acccggtgac
     9421 gacctcgccg gtaggggaga agagccttat cgtgacgaaa agggtctcct gcagcccctg
     9481 cctgaaagaa aagtgcgata ccgacttccg gtgcatggac ctcataacgg cggacgaagt
     9541 ctacgaagcg gcaaaggggt tgttggcatc cgttttgtga gggtcatctt tttagaagcg
     9601 ggagcgccgc agccgaaaac gaccggcggt agtggccccg gaaggaaaga gaatgaagaa
     9661 taacgtcgcc gtattcatgg accgggacgg gacgatcaac gaggaggtgg gttatctcgg
     9721 tcgggtcgaa gaactcgtct tgattcccgt tgccgcggag gccgttcgcc tggtcaacga
     9781 ggagggcatg aaggccgtcg tggtcaccaa tcagtccggc gtcgccagag ggttcttcac
     9841 ggaggaagac gttcgcctga taaacgggcg actcgcagag atgctgcgtg acgagggggc
     9901 cgtcatcgac cggttctact actgcccgca tcacccgacg gaaggaagag aaccctaccg
     9961 ggtcgcctgt gcctgccgga agccggaacc ggggatgctc ctccaggccg cggccgagct
    10021 cgacatcgac ctcgcccgct catacttgat cggggatatg ctgaaggacc tcgatgcggc
    10081 acaccgcgcc ggtgcaaagg gcgtccttgt caggaccggc tacgggaaga atgtcggcgt
    10141 cacggacggg ccggattaca tcgccgagga catcctcgat gccgtccgct ggattcttga
    10201 cgacaggaag aaggcacgcc atccttcgcc aggcccgaga taacgttatc gacggaagcc
    10261 aatgaacatc ctgatcgtaa aactgagcgc cgtcggcgat gtcgtccaca ccctgccgtc
    10321 tctggcggcg ttgagaaagc tctacccgga cgcccatatc acgtgggtta tcgaggaagc
    10381 cgcggccgac ctcgttaccg gccatccaag cctcgacagg gtgctcgttt cccgccggaa
    10441 aagctgggcc aaaagtctcg ggagtccccg gcgcgccctc cgggcggttg atgaagtaag
    10501 gtcgttcctg cgtacgctca gagaccgccc ttacgacctc gtcatcgatt ttcacggcct
    10561 tttcaagagc gccgtcatcg tcttcctcag ccggggaaaa cgcaaactcg gttacgacag
    10621 cctccaggaa ctgagcggcc tctttctgaa cgagaagatc cccgaggaca tggacaaaca
    10681 cgccgtcgac cgttatctcg acttcttgcg tcatctcggg gctcccgagg aagaacccgc
    10741 ctttttgatc cccgtcggcg aggaaaacag gaggcgggtc aaaggcttcc tcgaagagta
    10801 cggccttgac aggggcgcgg gatttgtggc agtaagtccc gtggctctgt gggagacgaa
    10861 actgtgggag gaaaacagat tcgcggccct atgtgacagg gtcgtcgggg aactcggcct
    10921 gaaggtcgtc ctgactggtg ataaagccgg aggtgttctc ggtcgcatcg aagcccggat
    10981 gacggaatcg gcagtgaacc tgggcggaaa gacgacgctg cgcgacctgg cggccttgta
    11041 taaaagagcg agggtcctcg tcaccaccga ttccggtccg atgcacatcg cggccgccgt
    11101 cggcaccccg gtcatcgccc tcttcggccc cactgcgccg tggagaaccg gaccatacgg
    11161 cccgggccac atcgtcatcc ggaaagcgat agcctgcagc ccctgctttc taaaacactg
    11221 cgcaaccggg cagtgcatga aagacatcac ggtggaagag gttttcgaag ctgtaaaaaa
    11281 gggcatcgcg gacgtataaa gcccatgaat aaggacctca catgaaacca cgcatcggct
    11341 ttactttgtc gttcttcgtt gtcgttttcc tgaacctcat agtcttcata ccctttgtat
    11401 tcgctgagga tcgcgtcagc acagccgcgg cgaacccaga gactcgcttc tggaaagagt
    11461 cctgtgtcgt cataggttac ggaacaagcg agattcacga gggccattat gaaccgatgc
    11521 tgttcatctg gcaccttgcc tacgacctca ggaattatat cccggcactg aagaaccata
    11581 agggaatcct ggccggcgtc tgcgagcccc aggtcaaccc ggccttccgg ccgagctcgg
    11641 actttgaatt cggcgtgggg gtggggctga agtacttata ccccgtgacc gacaggctct
    11701 acccatacat ccaattgacc gtcgggccgc attatatctc cctgcaaaca gaagaccagg
    11761 cgagtgggtt tatcttctcc aacactgtcg gcgtgggaat ttactacttt gtcaccaaaa
    11821 actcggctgc catggtcggg tatcgtgctc gtcacatatc caacgccggg acaagggaac
    11881 cgaacgacgg gatcaacacc catttcggca ccatagggta ctctttcttc ttctgaggat
    11941 tacggcacgg aggcgaaaat ggtgatcagc aaagagctgc tcgacatcct ggcctgcccg
    12001 aactgtaagg gcgacatccg tcttaatgag gccggcgacg gcctggtctg cgatcattgt
    12061 aagttgctgt acgagatcaa agacggcatc ccgatcatgc tcgtcgagga ggcgaaaaaa
    12121 atcgaatgat tcacgggcac cctcgcagat gccagaggat gagtcgcgag tagacggaca
    12181 acttagtcct gattcttctg ttcgccccga gcaggaagag attcgccgcc gccatcgaaa
    12241 cccaatcgac ggtcagccgg caaagaatca ataatcgggc aagaatatat tggggtcgtt
    12301 tgtaccattt gcgaaagtaa gcgtaccgag accgatagaa agcgatcctc gactcgacgc
    12361 cgtggccgat actccggcct tgaaagtggt agatcgcggc cgaaggaata aaataaacct
    12421 tccaaccggc cttcttcatc cggaaggccc aatccgtctc ttccagaaag aagaaaaacc
    12481 gctcatcgaa cagccctact tcgtcgattg ccttctttct caccatcagg caggcgccga
    12541 tacatgattc tacctcaacg ggttccttat aatcctttcg tttactcggg tatcttttag
    12601 gaaaaaggta ttccaggata gaggtgttga agaggagggt taaaaggctg gggaaggcgg
    12661 cgatggaatt ctgcctgctc ccgtcttcgt tcaacaactg cccgcaggcc atggcggtgt
    12721 cggggtgctg ctctaaaaag aaaaacagtt gacgcaccgc gccgtccgtg aggacggtgt
    12781 ccgtgttgag gaggagggcg tagcgtccct gcatcacctc cagggcctgg ttgtttgccg
    12841 ccccgaaacc cctgttcgtc tcgttctcga tgattttgac gtcggggaaa tcctctttca
    12901 gcatggcgac gctcccgtct tgcgacgcgt tgtccaccac gatgacctcg aagtggatgt
    12961 ccttcaaggt tcgatagacg gactggaggc agccgcgcag gaggtctttc gtgttccagt
    13021 tgacgatgac aaccgagagg tccattcaaa cctttttcgc cccgcgactg agttcgctca
    13081 gttttgcgta cttgaagaat ttgtttaccg agtcggtgac ggagaggatg aaaccctgtc
    13141 tcccgtcaag gaaaccgagc ctcaggatgt agttctgaag gaaggccaac tccgcccgca
    13201 gtaccgcctc ccctatcgag gccctccggc cctcggcaaa cgcctcctgt gcgcccaggg
    13261 tggagtaacg gtcgatcttc atcaagattt tgctgaaacg tccctcggtg aaatgctcga
    13321 tgggtacgtc gagggctgcg accgggccgt ccacctgcac cgactcatgg acggtggcct
    13381 cactcatccg gccgacccct cttcggaaga gcctgacgat ccggtccggc caccagccca
    13441 tgtgtctgac ccagcggccc tgaaaataat ttttcctcgg gaagctgtaa cccgccgcac
    13501 ccttatcttg tcccgtgaca atgcctttga tggcctctgc cgtctcggcg gggatgcgtt
    13561 catcggcgtc gaggaccagg atccagggat gccgacaccg gtcgacggcc gactgcttct
    13621 gggggccgaa acccctccag ggtacctcga acacctcgca accgaggtcg gaagccagtt
    13681 gaacggtgcc gtcggtgctc cccgaatcga cgacaacgat ctgtccggcg aaagaaacac
    13741 tcctcaggca gtcgggaagg ttttcggcct cgtctttcgt tatgatcgcg acggaaaggg
    13801 ggatcttatc tgtcatagga agaatttctc gtgccaaagt acacccgctc gcccttgatt
    13861 cgtctcctat catcacaggg cgaaaggatt atcagattag tgggatcaat gcaatgtcgt
    13921 gaaagtccgc gggcagtgca tcccgtcgcc ccttcttctg tcgcatcttt cctaattcct
    13981 tgcgaatcct cctgcttgtt ggtatcctca ccgcgctcgg ccgccttatc ttgtcgtgtc
    14041 ggtttgtccc tccgggcagg catcacatct cagtgcaggc cgcttacttt tttacggggt
    14101 ttctatgaca tcggcattca aaacagcgaa gacggccgaa atcgatcgga cgaagttggc
    14161 tgaatattcc gtgccggttt tgttggccgt ctatattttt gcccagccgt tccattacct
    14221 gaccgctgta gaggaagtgg ggatttatct cgccttttta atatcggttt atttggtcct
    14281 taccaagaaa gtcgagttta ccttcgatac gcctttgtct ttgccgttcg tgctcttcgc
    14341 cctgtgggcg ctgataggga ttgccttcgc aataaacaag ccaaattcca tccacgactt
    14401 ctatgcccat ctgttgaaat actatgtgtt gttctttttg gtgttcaatt ttttcaggac
    14461 tcgagataag tttttggccc tggccctgat cataaccgtt tccttcacca ttttttcttt
    14521 gtgggggatg gtatgtgtat atggcgtaga aggcaaaaag ctcttcgtgg acaggttcac
    14581 cagccccgcc tttctccctt atcgaacgta catccatgtc ttcggagcga ttaccgctat
    14641 gagtttcttt tactccgaaa cggctgtctc gaggaaatcg ttttttgccc tctgcatatt
    14701 tgtcgatgtc ttggcgactt tcttatccca gacaaggagc gcaatcatag ccttgacggc
    14761 cgcactgtac ctgttgctca tcgtcaagag gaagtacaaa cagatagtac ttattacggt
    14821 gctcttgttg gccgtagtct atttctcacc ggtcaaggat cggtttctgg acgaaaggga
    14881 atacactgtc agaatagcca attattacct gtactctgca atcatcaggg actatcccat
    14941 aaaaggtatc ggtttcggta tgcagaccta ccaggacccc gggttgaata tcgattcgta
    15001 caacaagtat gtgcctgaga agatacgggt atcccctccg aatctgatcc cgcacaacac
    15061 attttttgac atagccgtga ggttggggtt gatcggcttg gggctttatg tgttgataat
    15121 tttcatgtcc ttgaagatgt tgtttgtcgt catcaggaat agagcggacc cgtttctccg
    15181 ggaatggggt ttttgcctct gcgccaattt cttggctttc ttcatccagg cgatgtttac
    15241 agaaacgtcg ttcggagtcc aagcccttac gcagtacttg ctgttcgcga tgatcgcgat
    15301 actctggaaa atcgacaagg agggcgtggg gacagttggt gagtaagaaa gatgaaaaat
    15361 ttctcttgac tcaaaacaca gttactctgt atttctgcac atcctgctac ggtctccaag
    15421 gatgcgatac gcccatgcag tgaggggcga tgataatgat aagcagttgc ggggtctttc
    15481 ccgcgagccg ttatctttcg ggttattcag acctgacgac tattttaatc gggaggccaa
    15541 aatgatcaga gaccttgtcc ttaagaacag gagttaccgc agattttacg aggacttttc
    15601 ggtcgacagg gaaaccttgc aggagcttgt ggacctcgcg cggcttaccc catcggctgc
    15661 caacaaacaa cctttgaagt acatcctttc ctgcgaccgc gagaagaacg ccgtcatctt
    15721 ccctaacctg gcgtgggcgg gatacctcaa agactggccg ggaccctctg aaggggagag
    15781 accttcggct tacatcatcg tgctcgtgga cacggaaata agtcggtccg cggacatcga
    15841 ctgcggcatt gccgcccaga ccatcctctt gggcgccgtt tcgaaggggc tcggagggtg
    15901 catgatcgcc tcagtccggc gggacagtct ccgggaggcc ctcaaaatac cggcgcggta
    15961 cgagatcatc ctcgtcatcg ccctcggcaa acccaaggag accgtgatca tcgagaagat
    16021 gggttccgac ggtgacgtga agtactggcg ggacggcgaa gggaggcacc atgtccccaa
    16081 acggtccctc gaggagatca ttctgccctg atccgtttca gggtggggga tgaccccggg
    16141 cgacctcgct cacagtcccc tggcagcgat gtaagcggcg aggtcggcca cgcggcagga
    16201 gtaaccccat tcgttgtcgt accaggcgac gcatttggcc aggttgcctt tcccgcggag
    16261 tacctgcgtg aactcgacgt ccaccgtcga cgaatgctca tcgcccttga agtccgtcga
    16321 gaccagcggc tcctcctcgc aggccatgat ccccttcagt cgtccttcgc aggcctcctt
    16381 caagacggcc ctgagttcct ccgtcgttgc ccctttcttt agctgggccg agaagtcgac
    16441 gacggaaacg gtcggcgtgg gaacccggag ggcgtagccg tcgaaccgac ccgccaggtc
    16501 cgggatcacg agggcgatcg ccctggccgc cccggtggtc gtcgggatga tgttctgtgc
    16561 ggcggccctg gcccgtctca ggtccttgtg gggaaggtcg aggatgcgct ggtcgttagt
    16621 gtaagaatgg acggtgacca tcatcccctg ctcgacgccg aaggcctgat ggatgacgga
    16681 taccggcggc gccaggcaat tcgtcgtgca agaggcgttc gagaggatgt ggtgcttgag
    16741 ggggtcgtag gcatcctcgt tcactcccat gacgacggtc aggtcttcgc cctttgccgg
    16801 ggcggtgatg atgacttttt tcacgcccgc cgcccgatgg acggccgctc tctcggccga
    16861 ggtgaaaagg ccggtgctct ccactactat gtcgacgccg aactcgtccc agggtatctc
    16921 cccggggtcg cggcaggcga agctctgcac gggttggtcg ttgatgagaa agcgatccct
    16981 ctccgcgacg acggttccgc cgaaccgtcc gtagttggtg tcgtacttaa ggagatgggc
    17041 gttcgtcttt gtgtcgaaaa ggtcgttgac ggctacgacc tggagggtgg cggcttgcct
    17101 ttccatgatc gccttcagga cctgccgtcc gatcctcccg aaaccgttta ttcctatgcg
    17161 gaccatgttt tctgtgtctc cctgttggtt cagtcaatca gtaattgccg gcctgaggca
    17221 ggctgttcag ttgtcgtcca gacggtactt ggactccgcc atcaggtcgc gctcccgtcg
    17281 gagatgctga tgcatcctgg cgttgtccag ggcgatggcg ctcaggttag ccgccgcctc
    17341 cataaaccgg atttccttct ctttgaacct tttcacgtca tccgtgtaaa cacgcaaaac
    17401 cccgactgcc gtggtcccta cgacgagagg aacgactagg acagaccgga tgccttccgc
    17461 gcgggccatg tcgttgtact ggaatcgcgg gtccgtctgg gcgtcttcga tccagatgac
    17521 cttccccttg agcgcttcct ggtctatgcc gctttcctcg acgaggacgg gtcctttgtg
    17581 gacgtaatcg ctcgaaaggc cgtggtaggc ccccaggttc aaccttttct tccgctcgtc
    17641 gagtatccgc agtgagctgg ctttgacctt catggcctcg gcaacgctac ggacgatctc
    17701 ctccaagacg cgggccggct ccagggatgc gtttatcacc ttcgctacat cgtagagcgc
    17761 ggtaaagtaa tccatctctt tttcgggcat tttgcctctc ctgtttttgg tgtcccgata
    17821 acgattgaca atcttnnnnn nnnnnaggca gcaaatccac cttaaacatc atgccggtgc
    17881 gtcatcccga tgaaaatcgg ggcggcatca aggcgaatgg ccttcaacgt cataccggcg
    17941 acagccggta tccagggctg tcagctggtc acaacatcgc tggcccccgg ccttcgccgg
    18001 ggtgacgact ttatacgaga acttcacgat cgacgggatc gtgaaatgta aaaatcggtt
    18061 tttcgtcaag cttcgacaag actgccatcc ctcaccgcac agccactgtg acggtttggg
    18121 cgtcgaggac ccgcagtaac tccgtcggcg ccatttccgc aaggagcccc tttcttcccg
    18181 cgttgatgag tattttcggg aggtcgagga ccgtggcttc cacgtaaacg ggcaggcgct
    18241 tgcgcgtccc gaagggcgat gtccccccga ccatgtagcc ggtgtgtttt tccgcgacct
    18301 tggggtcgca ggcgctcacc gacttgacgc ccagaaacct cgccatgttt ttcgtcgaga
    18361 cgtgcctgtt gccgtgcatg atgacgatga agggtgactt tcgctcgtcg tcctccatga
    18421 cgatcgtctt cacgacccgg tactcatcga ctctcagttc cctggccgcc gtctcggtgc
    18481 atcccctctc ctcgtatttg tatgggtgga gcgtgaaggt aacccctttt tccttcatca
    18541 tccttatcgc cggcgtcgcc gggaagtgat ctttggtcaa ttctactcct caaaaaggcc
    18601 ggaaggcaaa gggctaaaaa gacatgcccg gcttccagct cttcccctgc catgtattcc
    18661 tttcttccat agcctcgcgg atggcccgaa ggttcgcctg tttttccagg gcccactgcg
    18721 gcgcgaccag ttccgccggg tgggggtcgc cggtcagacg atggatgact acggaaggcg
    18781 gcagcagcga aaggaactcg gcgacgattc ccgcgtattc ctcgcggtcc agacagcgat
    18841 aggccccgga ttcatatagt ctgtggagga ccgtaccctt tacgacgtat aggaggtgaa
    18901 tcttgactgc ctggatgtcc atcccggcga gggcctcggc cgtttcgagc atctcgcgtc
    18961 tcccctcgcc ggggagcccg aggatgacgt ggacgcagac gtcgacccct ctccggcggg
    19021 ttctgtcgac ggcctcttgg aagaccgaga aggtatggcc cctgtttatg agttcgaggg
    19081 tacggtcatg ggcggactga aggccatact cgacagagac aaaggaacgc cggttcagct
    19141 cggcgaggag gtccaagact tcgtcgtcga cgcagtccgg cctcgtgccg acggcgaggc
    19201 cgacgacttc cggaacgcca agggcctcct cgtaacggcg gcggagcgtc tccacgggcc
    19261 cataagtgtt cgagaaggac tggaagtatg cgatgaattt ctccgcctta tacctttttt
    19321 ccagggcggc ctttcccctc tcgagcttct cccggaccga gagggacgag aaggcgccgg
    19381 tcccggaacc gcgggtgttg cagtagatgc acccgccggt gccgaccttt ccgtcgcggt
    19441 tggggcaggt gaaaccggcg tcaagagtga ccttctgtac gcggcagccg aaacgctccc
    19501 gcattgcccg ggcgaggtcg ttgtatggca aacttgacat ggctcttcga ggatatataa
    19561 tcaaaaaccc ttcgctatcg aacggataca ttctggcttc ataccctgga attgtcaacc
    19621 cggaagttcg attttggagg aatcaaacgt ctttacgccg ggacctctac accggagatg
    19681 gccttcggga ggggtccgcg gacttttttt ggaccgtgag ccttggaatg tcttctcagt
    19741 atgacgtgat cgtcgtcggt gcgggccatg cgggctgcga ggccgccctc gcggcggccc
    19801 ggatggggtg ccggacgttg ctcttctcga tcaatctcga caccgtggcg catatgccct
    19861 gctcgccttc gatcggcggc gtgggaaagg gacacttggt gaaggaagtg gacgccctgg
    19921 ggggcgagat ggggaaggcg gcggacctca cggcccttca ataccgaacc ctgaacaccc
    19981 ggaaaggccc ggccgtccgg agcacccgca cgcagaacga caaggcccgt taccggatac
    20041 tcatgaaggg cgtggtcgag cgccaacccc tgatcgacct gaagcagacg ctcgtcgagg
    20101 agatacttgc cgagggcggc agggtcgtgg gggtccggga tcatatcggc gcggagttct
    20161 tcgcaccgac agtggtcctg acgacaggga ccttcctcca cgggctggtc catatcggga
    20221 ggaagcagat cccctcgggg agggccgggg agtttccggc cgatggcctg gccgagagcc
    20281 tgaggaacct cgggttcagg atggggagga tgaagacggg aacgcccgcc cgtgtccgcc
    20341 gggacaccat cgacttttgc cgtttcgctg aactgcgggg agacgaagag ccgaatccct
    20401 tctccctctt cacggaaaag acggaaatca cccagattcc ctgctacatc gggcgtaccg
    20461 gtgagcggac gggggagata atccggcgca acatccatct ctcgcccctt tacaacggga
    20521 ccatccaagg ggtgtcggcc cggtattgcc cgtccttaga ggacaagatg atgaagtttc
    20581 ctgagaggga gcatcaccag ataatcctcg aaccggaggg gctcgacacc gaagagatct
    20641 acgtgagcgg cacgggcaat tccttgcctt acgaggtaca gatcgacctc gtgcggtccg
    20701 ttcccggcct tgaagaggcc cgcatcatgc ggcctgccta cgccatcgag tacgactttg
    20761 tctaccctac ccagttgaaa ccgacgctgg aaacgaagct agtcgagggt ctgtttttcg
    20821 cgggacagat aaacggaacc tctggttacg aggaggcggc cgcccaggga ctctgggcgg
    20881 gcataaacgc ggccctcaag gtgcagaagc ggcggccctt catcctcgac cgttccgagg
    20941 cttacatggg tgtcatggtc gacgacctcg tcacgagggg gacccgcgag ccttaccgga
    21001 tgttcacgtc ccgcgccgaa taccgacttc tgttgagaga ggacaacgcc gcctttcgcc
    21061 tggtcgagaa gggtttcgag ctcggcctcc aggacagaga ggccttggaa agggtagtgg
    21121 agaaggaaag gcgcgtcgag ctggagacga gacggctgga gcagaagagg gtgaggtcga
    21181 cgccggaagc gaaccgcatc ctcgagaagg ccggctcggt tcccatcgac gaatccgcta
    21241 cgctcagcca gatactgaag aggcccgagg tgtcctatgc cgatgtcgag gcgatcgagg
    21301 gcgtcatttc cgacctgccc gcggacgtga agaggcaggt tgaggttcgt tgtaagtacg
    21361 acggctacat cagacgccag gaggcggagg tgaaaaagtt caaacacctc gaaaggctgg
    21421 agataccggg ggacttcgat taccgcggga taccggggct ttccaacgaa ctcaagcaga
    21481 aactcggcga gatacgcccc ctttccctgg ggcaggcctc gcgtatcgaa ggcatgacac
    21541 ccgccgccct ttccgtgctc atggtctttc tcaaacggca ccgggaagga aaagcatctt
    21601 gactgaacag gtttgctggt aaaccgcgtc gatgtatgtc cctcggctct tttccgtccg
    21661 gggtcattcg gcgccgctgc cgccgtcgag ccttaattct tccagcgccc tgaggacgtc
    21721 atcttcaccg ctgtaatgct tttttcccgc cctcccggcg atcacgacgg tgggagtgga
    21781 ccggacgccg tcttccttga tgtagtcgtt gtactttcgg aagaacgggg cggtgtcccg
    21841 ctgcacgact tttatctttt tcgcctcaag aaaattcctc aactcgcttt ccgttttcaa
    21901 tcccttttcc gccgcctcga agagcgcacg ccggacctgg ttggcggatt caagggtttc
    21961 tccccccgca ccggtagcgt agatgtaata ccttgcgtaa aggaccgttt cgcggtgcat
    22021 cggtgtatcg ataaaaagaa cccgtccctt tccgtcccgg gcgatctctt cggtcagctt
    22081 ctctatcttc ggttccacgc ggcgacaggg cgcacagaaa tagtccgagt aaacccggac
    22141 ctccctttcc ccctgcccgt aaacgtacgg gcccgtctcg gcggcatacg caggcaaggc
    22201 gctccccgaa aaggtcaaga caaagaaggc gaaccccgcc agcacgaata gtatgagggg
    22261 ataatgtctc gacgccggcg agaaccggac gcctccaagg aggtagagca gccgccgcca
    22321 tcccctttcg gcgggaggtt catagttgac cgcaaaggcc aaaaccacgc aggcggcgaa
    22381 ggcaaggcag taggggcaat aaacaccttc tttcaactgg aaccagagga ggaacacctc
    22441 ccctcccatc cccgcagcga gggcagctcg caaaaaatgg ctcctctccg cccaggcaag
    22501 gacggcaact acggccatga agagaatccc gaccaacttg aggtcgattc cgaggacgtc
    22561 gccctggaga taggagcact caccgtcgca aagggtgtag aaggccatcg tccctatgcc
    22621 gacgagcgca agcacgacag tcgccgtttt ctttgtccgg gaaaggcgcc tatccatctt
    22681 cggctctcaa gatttcccgg ccgctttttc tctgtaacgg gccatgatct ccgaccgggt
    22741 gaggatgggg actacaggac ggacgtgccg ctcgatcaac tcccggccgc cttcttcccg
    22801 gtcgatgagg acgacggcga gctctacggt cagccctgcc tcccgggcgc tttcgaccgc
    22861 cgtgatggtc gaggccccgg tagtcaccac gtcgtcgatg acggcgactt tatcgccagg
    22921 cctcacgttt ccttctatct tgctccgggt cccgtgggcc ttggcgtcct tccggacgat
    22981 gaatgaccgg atagggcgac ctttctcgaa ggaaatcagg gcgagggcgt tggctatcgg
    23041 gtcggcgccg agggtcaacc cccctgccgc cttcacgtcc gtgtccttca atatatcgaa
    23101 gacgaggttg ccgatgaggt acatcccttc cgggtcgagt gtcgtgggtt tgcaatcgaa
    23161 gtagtaaggg ctcttcctcc ccgaggacag cgtgaagggg ttatcctcgt tgtaccggaa
    23221 agaccgctcg atgatgaggt cggcgagcct gtccttcatt ttttcaaggt ctttgttcgt
    23281 catgtcttac cgaatatctc ctcgagtttt ctgaccgcct cgacgccgtc tttggcgtat
    23341 tcggcgccca gggatcgggc gtaggccttc gtcacgaccg cgccgccgag caggaatcgg
    23401 cagtcggaac actccttggc ggccagctcc atcacttcct tcatgcgtac catggtcgtc
    23461 gtcatgaggg cggacaaccc gacgaccgct cctctgtggg ccttcgctgc ctcgatgatt
    23521 ttccctgcgg gcacgtcttt ccccaggtcg atgaccctgt agccgtggtt tttcaccatc
    23581 agggcgacga tgttcttccc gatgtcgtgg atgtccccct cgaccgtcgc gaggatgacg
    23641 gtctttctgt cgtcggctgc aaggtcttct ttgaggtggg gttcgaggta atccatgccc
    23701 tgcttcatcg cctcagcgct cgcgatgagc tggggcagga aatactcccg cctgtcgaac
    23761 cttcccccta cctcggtgat cgccggtatc atcacgtcct caacgaggcc gaggggcgac
    23821 gcccctgcgg ccagggcggc ggcgagcaac cttcctatgt cctcccggtt gccctccagg
    23881 atggcctggt atactttttc ctccggtgtc gttgtttcgg gggtctctcg tttcccggag
    23941 tttgcggcct gttcactgaa acgggcgatg aaagctgccg cgtccccgtc cttctgggtg
    24001 aggaggtcgg aggcggcctt gatgttcatg agctccgcct ggccggggtt ggcgatggcg
    24061 agggtcagtc ccttcccggc agccatggcg aggaaggcgg cgtttatcca cattctcccc
    24121 ggcatcccga aggagacgtt cgagaggccg aggaccgttc ttgtgccgaa cctgcgggag
    24181 caccactcga cggtttccag cgtttccagc gccgcccggg ggtccgccgc gacggtcatg
    24241 acaagcccgt cgacgaccag gtcctccttc gtgaaccccc gcgcccgtgc cctccgaaag
    24301 acatcccgga tgacctcctt gcgtctcgcc gccgtccggg ggacctcccc ctttgcgacg
    24361 ggtaggagga tgaacatggc gccgtatctg gccgccgccg caagcaacct gtcgattttc
    24421 tcttcttcgc cggagattga attgagcaag gcccggcccg ggtatctcct cagagctgct
    24481 tctatcgtct cgaccttcgg cgagtcgatc acaaggggca gggcggtggc gaccgcgagc
    24541 acgtcgacgg cgccagtcat ggcctttact tcgtcgatgc cggggacgcc gacattgacg
    24601 tcgaggaggt ctgcgccttc cctctcctgg tcgcgggcaa tctggcggac cagggtgaac
    24661 cttccttccg tgaattcctc ccggagggcc tccctcaggg cctttttccc cgtggggttt
    24721 atccgctcgc cgatgacctt cagggcgcct ccccggtcga gaaagaccgt cttcctcggc
    24781 gaggtgaggg ctgcgatcga ccttttcgcc ggcaggatgg gtttcttgtt cttcaagttc
    24841 ctcttcaggg aacggatgtg gtcgggcgtc gtcccgcagc agccgcccac tagggcggcg
    24901 ccggcggagg caagctcctt tccgaaggct ccgaactcgg acgcgtccat cgggaagacc
    24961 gtctcccctt ccttgacgac cggtatcccg gcgttcggct tcgccgcgag ggggacggtc
    25021 gcgtggggct tcatggcgga gatgaaccgc atcatgtcct cggggccggt ggagcagttg
    25081 cagccca
//
LOCUS       DAOTMX010000002        14424 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_4099_length_14424_cov_4.080033, whole genome shotgun sequence.
ACCESSION   DAOTMX010000002 DAOTMX010000000
VERSION     DAOTMX010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 14424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14424
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4099_length_14424_cov_4.080033"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="P5573_00130"
     CDS             complement(<1..350)
                     /locus_tag="P5573_00130"
                     /inference="COORDINATES: protein motif:HMM:NF018675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HRT26504.1"
                     /translation="MASEDGGQASDLKSSLLKEGRRYSFTQALRLLHYVLHKKAGPGV
                     HEQGVEKRIRVRPELSLAFPETDVASIEEFPDRPGRFLVTATFLGLYGTSSPLPTFYT
                     EDLLLERARDQSVS"
     gene            complement(314..2047)
                     /gene="tssF"
                     /locus_tag="P5573_00135"
     CDS             complement(314..2047)
                     /gene="tssF"
                     /locus_tag="P5573_00135"
                     /inference="COORDINATES: protein motif:HMM:TIGR03359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HRT26505.1"
                     /translation="MFNKYYQQELQNLRELAVEFSKVHPSIAPLLSGPTSDPDVERLL
                     EGVAFLTGLLRRKLHDDFPEIIHGLMNIIFPHYIRPIPSCSIVVFSPKPGMMETINVQ
                     KGVSLGAVPIEGTSCTFRTCFDLDVHPLRLISADIARQTGKPDVIRIVLETTGPDLSQ
                     WQPKKLSFFLGGSYSESTDLFMILTRYVRQIVVRPSEKGSPCVFPKDALKPVGFDTEN
                     CMLPFPSLSFSGYRLLQEYFLLPRKFLFFELHGWETWQERGKGKRFEILFELMPSPIA
                     PPKVKSDNFILFGTPVVNLFPHEADPFLFDHRLEKIRIRPAGLDKRHYRVYSIDSVIG
                     HTQGSLARKEYVPLERFQHGGDDHPIYQANYTRSPIDNSPEVYLSLTYPPTGGEPERE
                     TISVSMTCTNGELAERLRLGDICMPTSDSPELLSFRNVIPPTSTVDPPLGSNASWRFL
                     SHLSLNYFSLADAENIKELLKLYAYPEGRDRAAVTANLKRIDGIANFQVSPTDRLLRG
                     MMMRGQNLEMTSRHDHFAGLGDLFLFGAVMDLFFGVYSSMNTFTQFHLKDSLTGERFV
                     WPVRMGDRPLI"
     gene            complement(2063..2500)
                     /gene="tssE"
                     /locus_tag="P5573_00140"
     CDS             complement(2063..2500)
                     /gene="tssE"
                     /locus_tag="P5573_00140"
                     /inference="COORDINATES: protein motif:HMM:TIGR03357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HRT26506.1"
                     /translation="MSFIMHEESLLERIRRLEKETETGSPRDVSRGIRSVIDHLQRML
                     NTRQGSVLIADDYGMPDFTNFPEDDLKATAGDVERVIGQMIEKYEPRLQKVRVHFESK
                     SGDMFDLRFKLEADMISPSEQGKSIPVVFETIVTSEGKVHVES"
     gene            complement(2574..3059)
                     /gene="tssD"
                     /locus_tag="P5573_00145"
     CDS             complement(2574..3059)
                     /gene="tssD"
                     /locus_tag="P5573_00145"
                     /inference="COORDINATES: protein motif:HMM:TIGR03344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
                     /protein_id="HRT26507.1"
                     /translation="MAMTIYMKIKAKKQGDIDGDCTQQGREKMILCYSMDHYVEIPRD
                     THTGLPTGQRIHKPLTVSTHMGKHTPKLFQACTTGERMDVEIDMFWINEKGQEEKYFT
                     IKLQEAIVVDAREWFPPTFLEENKPYKHMNDFSFTYETIIWTYVPDGVEAQDSWKKPA
                     I"
     gene            complement(3181..4668)
                     /gene="tssC"
                     /locus_tag="P5573_00150"
     CDS             complement(3181..4668)
                     /gene="tssC"
                     /locus_tag="P5573_00150"
                     /inference="COORDINATES: protein motif:HMM:TIGR03355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HRT26508.1"
                     /translation="MAEEEKKVQETKAAETAAEGVSLLDEIVQATRMKPTDEGYAITR
                     QGVQAFIDEILKPGKEVRVTQDVVDEMIAGIDQKLSEQMDMILHNETFQKIESAWRSM
                     KYLVDKTDFRENIRIEFMNVSKEDLLDDFSDAPEVTKSGLYKIAYTSEYGQFGGKPYG
                     LMVGNYEFGPGAQDIRLLKYVASVANMSHAPFVAAAGSSFFGLEDFSGLPNLKDLKAI
                     FEMPQYTKWQSFRESEDARNIGLVLPHFLLRLPYGPDSQPVKSFVYKEKLSEGNKNFL
                     WGNAAFAFASRVSDSFAKYRWCANIIGPQGGGAVEGMPVYQYEAMGDLQTKIPTEILI
                     SERREFELAEEGFIALTMRKGSDNAAFFSANSVQKPKVFANTPEGKQAETNYKLGTQL
                     PYNFIISRLAHYIKVIQRENIGTWKERGDLETELNNWIRQYIADMDNPSPAVRSRRPL
                     RKAEIEVSDVEGDPGWYRVSLKVQPHFKYMGASFTLSLTGKLDKQ"
     gene            complement(4679..5164)
                     /gene="tssB"
                     /locus_tag="P5573_00155"
     CDS             complement(4679..5164)
                     /gene="tssB"
                     /locus_tag="P5573_00155"
                     /inference="COORDINATES: protein motif:HMM:TIGR03358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HRT26509.1"
                     /translation="MAKEASVAPKERVNIVYRPATGDAKEEVELPLKILVMGDFTMSN
                     DGRPVEDRAPVNIDKDNFDEVLAASNAKLQAVVPNKLSDDPDAQLSVNLDFKRMKDFD
                     PDAIVEKVPELKKLMELREALKALKGPLKNVPEFRKKVQELVKDEGTRARLLRELGIE
                     S"
     gene            complement(5369..6940)
                     /gene="tssA"
                     /locus_tag="P5573_00160"
     CDS             complement(5369..6940)
                     /gene="tssA"
                     /locus_tag="P5573_00160"
                     /inference="COORDINATES: protein motif:HMM:TIGR03362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HRT26510.1"
                     /translation="MSLLGLGNTPISERNPSGEDIRLDPDFEVLSGEMEKMSSPSVIG
                     DLDWKKVVALSSDILAYKSKDILVACYLSVGLFRTEGLNGLASGVRILRDLLETFWES
                     FFPPLKRMKARRNAVEWWLEKVRSGIAGVKTEIWPAEKKAAFIDDLKAIDSFVGKNMD
                     DAPMMIPLITNISAVIAIEASAEEKPQDQEPPPVEEPPSPSEKASDRHTPLSDAETEK
                     PREVFPETDAEKLIKQGLDTLGRAAAMYARRDPLNPLYFRLNRVVAWIPVTSLPPNSE
                     GKTFIPPPDGEVVVALRNLHRSGSWKELLQSAESRVPEFLFWIDLSRYSAEALEKIGR
                     KDLSEAVSMETSSYVGRLPGVERLAFSDGTPFADENTREWLKDIGGQAQVRQDIPVGY
                     DTDELNEEIAKDAALALELLNANKLPEALNTLREKLNRASSARERFIREVGFCRLLAQ
                     TKQAGISDPYLQELLEHIDVYKLEQWEPSLAVEALATVLSSFHLRKGEKAEGSAKSIF
                     DRIAAIDPARALNLL"
     gene            complement(6994..10452)
                     /locus_tag="P5573_00165"
     CDS             complement(6994..10452)
                     /locus_tag="P5573_00165"
                     /inference="COORDINATES: protein motif:HMM:NF025689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein IcmF/TssM N-terminal
                     domain-containing protein"
                     /protein_id="HRT26511.1"
                     /translation="MWKKVIKILFIALLLGILAGLTTWLVFSKRWPWWVGVALMVGLI
                     GVCVGVLFLRKYLQRRREMVFVSRVIAQDEAAIKTAPIHERQRLQELQDRWKESVERL
                     RSSYLRKRGNPLYVLPWYLVIGESGSGKTSAIRNAGLSSPITEIGPTTGISATRNCDW
                     WFFDEAVILDTAGRYTIPIDEGPDKEEWKNFLALLARYRRKEPLNGVIVTIPADKLLS
                     PDESELREDGQNVRQRIDQLMRTTGAKFPVYLLVTKMDLIHGFVGFCNHLSGKDVSQA
                     MGSLNENLNPYWNEFLEHSLDVISNRLGELRLIMVHRKKPADPGSLFFPNEFECLKPG
                     IQVFVKAVFEENPYQETPLFRGLYFSSARQEGRPQSEFLSLTGIVPSANSGSTGENGL
                     FLRDIFKRILPNDRYLYRPLQEFLRWRTLTSNQGLLAWIMVWFSLCCFLTYSFFKNSV
                     TIGEFTKSFYTSPVLTGDMSSDLLMLDKLRMELMGMELKNRNWWVPRFGLDESLKIEQ
                     ILKRNFISIFDDGFLRPFDTRFDKHIENVSRHTPDDEVALYVSYIVNRINMYHEVLRG
                     EKPSFIARFDNDCIALITGEYPQIVPEIASKFDDIYYEYLTWNEHKGEFQGKIKELRV
                     TLKRLLEKRGGNLEWLVKIYIPNVPDVRLRDFWGATEEGRYNEQILVSRAYTGKGRKD
                     IQDFIVFMDEALTDEKVSEESKNKFWNWYWQHFYESWAGFTGQFSEGMDGLVTVSAWK
                     YMVALMTTDQNPYFLLLDRMADEFAQGEKGQLMPKWASLVLYLNDIRQMSKAKTEKTK
                     GTLTGRIESLKERLVERTKDIADKNEAKKHEEKLKIAKVWEEYRKTIEQLSPVATSLD
                     TCFGMVSDYFNPGKGEKQPLFLSAGNEGFKFKSMVRDEADASFVWDVVFGPYNFLVDF
                     SIMEAGCALQGMWEEQILGTLEGVGRDKASQILFNKSDGVVWKFLAGPAQPFVVKSKN
                     GYSARKKSGRSIPFNPEFFDFLNAGAENITVSRTDYEVSMKTLPLQVNKGARSEPYGS
                     IISVDCQDGKASLENYNYPQSKTFHWSPDKCGDVTLKILLPDIALTKTYEGQMGFAKC
                     LSDFRYGTRTFKAEEFPDEQKRLTDMGISWIRLSYWISGKKAVVQLLEKSSLQTPGEI
                     VPCWSR"
     gene            complement(10483..11157)
                     /locus_tag="P5573_00170"
     CDS             complement(10483..11157)
                     /locus_tag="P5573_00170"
                     /inference="COORDINATES: protein motif:HMM:TIGR03349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotU family type IV/VI secretion system protein"
                     /protein_id="HRT26512.1"
                     /translation="MNTRLVDCFMELITYTNYFLTMPKANQPSYDDTLNHYQQLFSRA
                     EEYARKGGFSAGEGESALFAVCAWIDESILCSDWSERGKWALSPLQLLRYNTTNAGEE
                     FFRRLGSLGQDERNVREVFEYCIVLGFRGRYFLTADEHYLKEISSGNLSLLTGSADPE
                     VPEKLFPGAYGAPIKTGRGKKWLRLISPSKIALIVISVIAFIALFLFYRYMLDRMVSG
                     FFSTGS"
     gene            complement(11197..12582)
                     /gene="tssK"
                     /locus_tag="P5573_00175"
     CDS             complement(11197..12582)
                     /gene="tssK"
                     /locus_tag="P5573_00175"
                     /inference="COORDINATES: protein motif:HMM:TIGR03353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HRT26513.1"
                     /translation="MEQRQPIFWYQGLFLQPQHFQQNDLYFQSLLHNLQAHRQPYFWG
                     VCKMDIFEPALNNRMFEISAGEFIFQDGTLVSFPDNAVVQPRSFKGLWAEEKPFNIYL
                     GLRKWNARGENVSVLNTPDAVSAAATRFISDVNPRELKDIHHGGPPAHIKFLNYLLKV
                     FWENEIEGAGDYELIPIACLEREMDDIKLSRRFVPPSVSVSSSGILAQTMKNIREKVT
                     ARCRMLEEYKVPREAQSSDMGFSFTIYLLALRSLNRYVPLLHHVTEAPAVHPWYAYGL
                     LRQLVGELSTFTDRVDALGRLRDGSPLMPDYNHFDIGGCFDEAQILIGELLNEITLGA
                     ENVIRLVREDGNFKASIPLDTFDSRNVFYLVVGTSQDREEVLPMLQNVAKVSSEEYMP
                     TLIKRALPGIPLEHSVTPMPGLPQRSGSLFFRIDRSSDHWLEIQKRQNICMNWNEAPE
                     DATVELIIVRK"
     gene            complement(12582..13235)
                     /gene="tssJ"
                     /locus_tag="P5573_00180"
     CDS             complement(12582..13235)
                     /gene="tssJ"
                     /locus_tag="P5573_00180"
                     /inference="COORDINATES: protein motif:HMM:TIGR03352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HRT26514.1"
                     /translation="MNGRSISPAERGKVRYGDIPLAGKPGSSSVVTLFALFAIISFFL
                     FTSCGTKTATDTAEMSLGKSVWSFAHGGIKIQYTADSMLNVFENEPHTLILSVYQTSN
                     INTFNNLTQDVDGLKKLLQGQSFDPSVVGVKNFIVQPAEEKIIVLNRAEGARWVGIVA
                     GYYALDPGKVSRSFNIPLITEKKGIYGFRKTESRAGQMTIKLFLGPSALHEREVVVK"
BASE COUNT         3411 a         3780 c         3491 g         3742 t
ORIGIN      
        1 ctgctgacgg actggtccct tgcccgttcc agcagcaagt cctcggtata aaacgtagga
       61 aggggcgaag acgttccgta tagtcctaaa aatgtcgcag tgactaaaaa acggcccgga
      121 cgatccggga attcttcgat ggaggcgaca tcggtttcag gaaaggctaa agaaagctcg
      181 ggccgcaccc tgatacgttt ctccacgcct tgttcatgca cacccggccc cgcctttttg
      241 tggagcacat aatggagaag cctgagtgcc tgggtgaagg aataacgccg tccctctttt
      301 agcaggctgc ttttcagatc agaggcctgt cccccatcct cactggccat acaaaccttt
      361 ctcccgtcag actgtctttc agatggaact gcgtgaaggt gttcatgctg ctgtaaaccc
      421 cgaaaaacaa atccatcacc gccccgaaaa gaaagaggtc tcccaacccg gcaaaatgat
      481 catgccgcga ggtcatttcc agattttgcc ccctcatcat cattccccgc agcaagcgat
      541 ccgtaggcga tacctgaaaa ttagcgatgc cgtcgatcct cttgagattg gcggtcacgg
      601 ccgccctgtc ccttccttca ggatatgcgt ataattttaa cagttccttt atattctccg
      661 catcggccag gctgaaatag ttcagtgaca gatgggaaag aaatctccac gatgcgttgc
      721 tgcccagagg gggatcgacc gtcgaagttg ggggaattac attccggaac gataaaagtt
      781 ccggtgagtc ggaggtgggc atgcagatat caccgagtct cagccgctcg gcaagctcgc
      841 cgttggtaca ggtcatggat acggatatgg tttccctttc cggctcgcca cctgtaggcg
      901 gatacgtcaa agaaagatac acttccgggc tgttgtcgat gggcgatcgt gtgtaattgg
      961 cctggtagat cggatgatcg tctccaccat gctgaaaacg ttccagtggt acgtactctt
     1021 ttcttgccag gctcccctgg gtatggccga tcacactgtc gatactgtat acgcggtaat
     1081 gtcgtttatc caagccggca gggcggatgc gaattttttc caggcggtgg tcgaacagaa
     1141 aagggtcggc ctcgtgagga aacaggttaa caacgggcgt accgaagagg ataaaattat
     1201 ccgatttgac cttcggcggc gcgatcggcg acggcatcag ctcgaaaagg atttcaaacc
     1261 tctttccctt tcccctttcc tgccaagttt cccatccatg caactcgaaa aagaggaatt
     1321 tgcggggaag gaggaaatac tcctggagga ggcggtagcc ggaaaaagac aaagagggaa
     1381 aaggcagcat gcagttttcc gtatcgaatc cgaccggttt taatgcatct ttcgggaaaa
     1441 cacagggact ccctttttcc gacggcctga cgacgatctg tcgaacgtac cgcgtgagaa
     1501 tcataaaaag atcggtggat tcactatagc tgccaccaag gaaaaaagag agctttttcg
     1561 gctgccactg ggaaaggtcg ggaccggttg tctccaggac gatgcggatc acgtcgggct
     1621 tgccggtttg ccgtgctatg tcggcagaaa tcagtcgcag cggatggaca tcgaggtcga
     1681 aacacgtcct gaaggtgcac gatgtacctt ctattggaac agcaccgagg gacacgccct
     1741 tttgaacgtt gatcgtttcc atcataccgg gtttcggtga aaaaacgacg atggaacagg
     1801 atgggatcgg cctgatgtaa tgtgggaaga tgatattcat taagccgtgg ataatttctg
     1861 gaaaatcatc atgcagcttg cggcgcagga ggcctgtgag aaatgctacg ccctccagca
     1921 gcctctccac gtcggggtcg gaagtgggac cgctgagcag cggtgcgata gacgggtgaa
     1981 ctttggaaaa ttcaacggcc agttcccgga gattctgtag ttcctgttga tagtatttgt
     2041 tgaacatggg agcaatctcc aatcaacttt caacgtgaac cttgccttcg gacgtcacaa
     2101 tcgtctcgaa cacaacggga attgatttgc cttgctccga aggactgatc atatccgctt
     2161 cgagcttgaa acgcaggtcg aacatatcgc ccgacttcga ttcgaaatgc acgcgaactt
     2221 tttgaagacg gggctcgtac ttttcaatca tttgaccgat gacccgctcc acatccccgg
     2281 ccgtagcctt taggtcatcc tcggggaaat tcgtgaaatc cggcataccg taatcgtcgg
     2341 cgatgagcac gctgccttgt cgcgtattta acatgcgctg caggtggtct ataactgaac
     2401 ggatcccccg agagacatcc ctcgggcttc ccgtttcggt ctccttctcc aggcgcctga
     2461 tcctctccag caagctctct tcatgcatga tgaaactcaa aggggacagc agccggtacg
     2521 ggcatccctt ttcttcatcg taacccggtt cctgtccccg attttaccgg aaatcagatg
     2581 gccggttttt tccaggaatc ctgagcctca actccgtcgg gcacataggt ccagatgatg
     2641 gtctcatagg tgaatgaaaa atcgttcatg tgcttgtaag gtttgttctc ctccaggaac
     2701 gtgggaggaa accattccct tgcatccaca acgattgctt cctgcagttt tatcgtgaaa
     2761 tacttctctt cctgcccttt ctcattgatc cagaacatgt cgatttccac atccattcgt
     2821 tcgcccgttg tgcatgcctg gaagagcttg ggcgtgtgtt tgcccatatg ggtgcttacg
     2881 gtaagcggct tgtgtatccg ctgcccagtc ggcagtcccg tgtgcgtgtc cctgggaatc
     2941 tccacataat ggtccatgct gtaacacaaa atcatctttt cacgtccctg ctgggtgcaa
     3001 tcgccatcta tatccccctg tttcttcgct ttgatcttca tgtaaatcgt cattgccatg
     3061 gctcaactcc tcctctcaca aagatgattt tgatttagcg caacaccctc ataaccggtc
     3121 ctcgttgttg caaagaatgg aatatggggc tgtcatccga ttaataaccc tctctgcctg
     3181 ttactgctta tccagtttac ccgtgagcga cagagtaaaa gatgcaccca tgtacttgaa
     3241 atggggttgc actttcaatg aaacacggta ccagcccggg tcgccttcta cgtctgaaac
     3301 ctctatttcc gcctttctca atggacgacg gctccttact gcaggtgatg ggttatccat
     3361 gtccgcgata tactggcgta tccagttgtt gagttccgtt tccaggtctc cccgctcttt
     3421 ccacgtcccg atattttccc gctggatcac cttgatgtaa tgggcaagac ggctgatgat
     3481 gaaattgtac ggaagctgag tgccgagttt gtagttcgtt tccgcctgtt taccctccgg
     3541 tgtgttggcg aacaccttcg gcttctgtac ggagttcgct gaaaagaaag ctgcgttgtc
     3601 actccctttc ctcattgtca gggcgataaa gccttcttcc gccagctcaa actcccgacg
     3661 ttcggaaatg aggatttccg tcggaatctt ggtctggaga tcacccatag cttcgtactg
     3721 gtacaccggc ataccttcca cggcgccgcc tccctggggt cctatgatat tggcgcacca
     3781 gcggtacttg gcaaagctgt cgctgacccg ggaagcaaag gcgaacgcgg cgttccccca
     3841 aagaaaattc ttattccctt ccgacaattt ttccttgtag acaaaactct tcaccggctg
     3901 ggaatcgggc ccgtaaggca ggcgcagcag aaaatggggg aggaccaaac cgatattccg
     3961 ggcatcctcc gattcccgga aagactgcca cttggtatac tgaggcatct caaaaatagc
     4021 cttcaggtct ttcaggttgg gaagtcccga aaagtcttcc aacccgaaga aggatgaacc
     4081 ggcggcggct acaaagggtg catgggacat attcgccacg cttgcaacgt acttcaggag
     4141 ccggatatcc tgggctcccg ggccgaactc ataattgccg accattaaac catatggttt
     4201 tcctccgaac tgcccgtact cgctcgtgta cgcgatttta tagagtcctg atttggtaac
     4261 ttccggcgca tccgagaaat cgtccagtag gtcttccttg ctcacattca tgaactctat
     4321 cctgatgttt tccctgaagt ccgtcttgtc gacgagatat ttcatggacc gccaggccga
     4381 ttctattttc tgaaaagtct cgttgtgaag gatcatatcc atctgttcgc tcaatttctg
     4441 atcaatgccg gcgatcatct catcaacgac gtcctgggtt actcggactt cctttccggg
     4501 cttgagtatc tcatcaataa aggcctgaac tccctgcctg gttattgcat atccctcgtc
     4561 ggtgggtttc atcctggtag cctggacaat ctcatccagg agggatacgc cttcggcggc
     4621 agtttccgct gccttggttt cttgaacctt tttttcttct tctgccatct tccccccctt
     4681 acgattcaat gccgagttct ctcaataatc gggctctcgt cccctcatcc ttgacaagtt
     4741 cctgaacctt cttgcgaaat tcaggaacat tctttaaggg tcccttaagt gccttcaggg
     4801 cctcccgtag ttccataagt tttttcagct caggtacctt ttctacaatg gcatcgggat
     4861 caaagtcctt catccgcttg aaatccagat tgacactgag ctgtgcatcg ggatcatccg
     4921 agagcttgtt cggaaccacg gcctgaagtt ttgcattgga agcggccagc acttcatcga
     4981 agttgtcctt gtcgatattg acgggggcgc ggtcttcaac cggcctgccg tcgttgctca
     5041 tcgtaaaatc ccccatgacg aggatcttca aaggcagctc tacctcttcc ttggcatcgc
     5101 ccgttgccgg tcgatatacg atattcaccc gttccttcgg ggcaaccgaa gcttcctttg
     5161 ccattttcca cctccttttt gtattgcata aacgtcagtt tcaaaaacaa atccgcactt
     5221 ttcacgcata ttttgtggtt attcccctgt ttcatgccat tttcaccaat cgagaacgga
     5281 tgttatcaca agagaatgct ttttcttata aaaaaactta aaaatctgtg gtccccttcg
     5341 ataaatcctt gctctcactc tttccccgtc agagcagatt gagtgctctt gccggatcga
     5401 tggcagcaat tcggtcaaat atgctttttg ccgacccctc ggccttttcg cccttccgga
     5461 ggtggaaact ggacagcaca gttgcaaggg cttcgacggc caaagagggt tcccattgtt
     5521 ccagtttgta gacgtcgata tgttcgagca gttcctgcag gtaaggatcg gaaatgcccg
     5581 cctgctttgt ttgagcgagc aggcggcaga aaccgacctc ccggataaaa cgctcccggg
     5641 ccgatgacgc ccgattcaac ttttccctga gagtgttcag ggcctcgggt aatttatttg
     5701 cgttcagcaa ctccagggct aaggcggcgt ctttcgctat ttcttcattc agttcatccg
     5761 tgtcgtaacc gaccggtatg tcctgtcgca cctgtgcctg accgcctatg tctttcagcc
     5821 attcccgcgt gttttcgtcc gcaaaaggag tgccgtccga aaatgcaagt ctctcaactc
     5881 ccggaagcct ccccacatat gatgatgttt ccatcgaaac cgcttcggaa aggtctttac
     5941 ggccgatctt ttcgagagct tccgccgagt aacggctcag gtcgatccag aagagaaatt
     6001 ccggaaccct cgattccgcg gactggagaa gctctttcca gcttcccgat cggtgtaggt
     6061 tacgcaatgc gacaacaacc tcgccatccg gtggggggat aaacgtctta ccttccgagt
     6121 tgggtggaag cgatgtcacg ggaatccagg caacgacccg attgagtctg aaatacagcg
     6181 gattaagggg gtcccgccgc gcatacatcg ccgccgccct gcccagcgta tccaagccct
     6241 gcttgatgag tttttccgca tccgtttccg gaaaaacctc ccgtggtttt tccgtttctg
     6301 catcggacaa cggcgtgtgt cgatcactcg ccttctcgga tgggctaggc ggctcctcta
     6361 cagggggcgg ctcctgatct tgcggtttct cctccgcact tgcttctatg gcgatcaccg
     6421 ccgaaatatt tgttatcagg gggatcatca ttggggcatc gtccatgttt ttcccaacga
     6481 aggaatcaat ggctttcaag tcatcgatga acgcggcctt tttttctgcg ggccagattt
     6541 ccgtcttcac accggctatg ccggacctca ccttttccag ccaccattca actgcgttcc
     6601 tccgagcctt cattcttttc agcggtggga aaaaactttc ccagaatgtt tccaggaggt
     6661 cgcgaagtat gcgtactccg cttgcgagac cattcagacc ttccgtgcgg aacaatccga
     6721 cggacaaata acatgcgacg aggatatctt tggacttgta ggccaaaata tcggacgaga
     6781 gagcaacgac ttttttccag tccagatccc ctatcacgga aggggatgac atcttttcca
     6841 tctctcccga cagcacttcg aaatccgggt cgagacgaat gtcttcgccg gaaggattgc
     6901 gttccgaaat cggagtgttg ccgagtccca aaagtgacat actcttcttc tggcggaatc
     6961 ccgctcagtc tccttttttc aaattttctc catctatctt gaccagcagg gtacgatctc
     7021 cccgggggtc tgcaacgatg atttttccag caactggacg actgcctttt taccggaaat
     7081 ccagtaagac aggcgtatcc aagaaattcc catatcggtc aaacgtttct gttcgtccgg
     7141 gaattcctcg gctttgaatg tcctggttcc gtacctgaag tccgataggc acttggcaaa
     7201 tcccatctgc ccttcatacg ttttcgtaag tgctatgtcg ggcagcaaaa tcttcaacgt
     7261 cacgtccccg catttatcgg gactccagtg gaatgttttg ctctggggat agttatagtt
     7321 ttccaaggaa gccttgccgt cttggcaatc gacggagatg atgctcccgt acggttcgga
     7381 acgcgcgccc ttgttcacct gtagaggtag cgttttcatc gaaacctcgt aatcggtacg
     7441 ggaaacggta atattttccg caccggcatt gaggaaatca aagaactccg ggttgaaagg
     7501 aatcgatctg ccggactttt ttctggcact gtagccgttt ttacttttca caacaaaagg
     7561 ctgggcggga ccggcaagga atttccacac caccccgtcg gatttattga agagaatctg
     7621 tgacgcctta tctcgtccga ctccctccag ggtccccaaa atctgctctt cccacatacc
     7681 ctgcaaagcg cagccggctt ccatgatgct gaaatcgacg aggaagttgt aaggaccgaa
     7741 cacaacatcc catacgaaag aggcgtcggc ctcatcccgc accatgcttt taaacttaaa
     7801 tccctcattg ccggcggaga gaaacagggg ctgtttttca cccttaccag gattaaagta
     7861 atccgaaacc ataccgaaac aggtatcgag agatgtggct accggagaaa gttgctcgat
     7921 cgtctttcta tactcttccc atactttggc aatcttgagt ttctcttcgt gcttctttgc
     7981 ctcattctta tcggcgatgt ccttcgtcct ctcgaccagt ctctccttga ggctctcaat
     8041 tctgcccgtc agagtccctt tggtcttttc cgtctttgcc ttcgacatct gtctgatatc
     8101 gtttagatac aacaccaatg acgcccactt cggcatcagt tggccctttt ctccctgggc
     8161 gaattcgtcg gccattctgt cgaggaggag gaaatacgga ttctggtccg ttgtcatcaa
     8221 cgcgaccata tatttccatg cggaaacggt aaccagaccg tccatccctt cactgaactg
     8281 acccgtaaat ccggcccatg actcgtagaa gtgctgccaa taccagttcc agaatttatt
     8341 tttgctctcc tcgcttactt tttcatccgt aagggcctca tccatgaaaa ctataaaatc
     8401 ctgaatgtct tttctccctt ttccggtata agccctcgat acgagaattt gttcattgta
     8461 ccttccttcc tcggttgcgc cccagaaatc acgcaaacgc acatccggaa cgtttggaat
     8521 gtatattttt acgagccact ccaagtttcc gccccttttc tctagaagcc ttttcaaggt
     8581 caccctgagt tcctttattt ttccctggaa ttctcctttg tgttcattcc aggtcaggta
     8641 ctcatagtag atatcatcaa attttgacgc aatctccgga acgatttgag gatattctcc
     8701 tgtaatcagg gcaatgcaat cattgtcgaa ccgtgcgata aaagagggtt tctctcctct
     8761 taagacctca tggtacatat taatccggtt tactatatag ctcacataca atgccacttc
     8821 gtcgtcaggg gtatgccgtg agacattttc gatgtgttta tcgaaccgag tatcgaaggg
     8881 tctcaggaag ccgtcatcga atatactgat aaaattcctc tttagaatct gttcgatttt
     8941 caaactttca tcaaggccaa aacgagggac ccaccagttc ctgtttttca gttccattcc
     9001 cattagttcc atccggagtt tatccaacat caacagatcc gaactcatat cgcctgtcag
     9061 gacaggggat gtgtagaagc tttttgtgaa ttccccgatg gtcacagaat tcttgaaaaa
     9121 agaataagtc aaaaaacagc atagagagaa ccataccata atccaggcca gcaatccctg
     9181 attgcttgtc agggtacgcc agcggagaaa ctcctgcagg ggacggtaga gataccggtc
     9241 attgggcagg attcttttga atatatctct cagaaagagt ccgttttccc cggtgcttcc
     9301 tgaattggca gaaggaacaa ttcctgtaag actgagaaat tccgactgag gccgaccctc
     9361 ctgacgggcg ctggagaaat agagcccccg gaacagtgga gtttcctgat agggattttc
     9421 ttcgaatacc gccttcacaa atacctgtat tccaggcttc aagcattcaa actcattcgg
     9481 gaaaaacaga gatccggggt cagctggctt tttcctgtga accatgatga gacgcaattc
     9541 tccgagccgg ttcgaaatca catcaaggga gtgttccagg aactcattcc agtaggggtt
     9601 taggttttca ttaagagaac ccatggcctg ggaaacatct ttccccgaaa ggtgattgca
     9661 gaagccgacg aagccgtgaa tcagatccat cttcgttacg aggagatata cggggaactt
     9721 cgcccccgtc gtgcgcatga gctggtcgat tcgctgacgg acgttttggc cgtcctccct
     9781 cagttcactt tcatccgggc tgagcagctt gtcggcggga atggtaacaa tgacgccgtt
     9841 caatggttct ttgcgccggt acctcgccag gagagctaaa aagttcttcc attcctcttt
     9901 gtcaggtcct tcgtcgatgg ggatggtgta ccggcccgcc gtgtcgagga taaccgcctc
     9961 gtcaaagaac caccagtcgc agttacgggt cgctgagatg cccgtcgttg ggccgatttc
    10021 cgtgatgggt gaacttaggc cggcgttcct gatagccgag gtttttcccg acccggattc
    10081 gccgatgaca agataccatg gcaacacata aagggggttt cctctcttgc ggagatatga
    10141 gcttcgcaag cgttctactg actccttcca acggtcctgc agctcctgta acctctgtct
    10201 ctcatgaatc ggtgctgttt tgatggccgc ctcgtcctga gcgatcaccc gggagacgaa
    10261 taccatttcc cgcctccgct gaaggtactt ccttagaaag agcacaccca cacaaacgcc
    10321 gatgaggccg accataagag cgacgcccac ccaccacggc catcttttcg agaagacaag
    10381 ccaggttgtg aggccggcga gaatcccgag gaggagagcg atgaataaga ttttaataac
    10441 ttttttccac atgcaacaat tccctattat gcacgggcgg tttcaggaac cggtgctgaa
    10501 aaaaccggat accattcggt cgagcatgta tctgtagaac aaaaaaagcg ctataaaagc
    10561 tataaccgat atgactatga gggcgatttt cgaaggtgag atgagacgaa gccatttctt
    10621 gcctctcccg gttttgatcg gagcgccata ggcccccgga aacagttttt ccgggacttc
    10681 agggtccgcc gatcccgtaa gaaggcttag gttgccacta ctgatttctt tcaggtaatg
    10741 ttcgtctgcc gttaggaaat aacgtcccct gaaccccaga acgatgcaat actcaaagac
    10801 ctctctgaca tttctctcgt cttgtcccag gcttccaagc cgtcgaaaga actcctcgcc
    10861 tgcgttagtc gtgttataac ggagaagctg aaggggtgag agcgcccatt ttcccctttc
    10921 ggaccagtcc gagcagagaa tgctttcgtc gatccatgcg caaaccgcaa aaagggcgga
    10981 ctctccttcc ccggcagaaa aaccgccttt tctcgcgtac tcctcggccc gggaaaacaa
    11041 ctgctgatag tggttcaggg tgtcatcgta agaaggctgg ttcgccttcg gcatcgtaag
    11101 aaaatagttc gtatacgtga tgagttccat gaaacaatcg acaagccgcg tattcatttc
    11161 tccgtatatt cccgtatatt aatgagacag ggtttatcac tttcttacga ttatcaattc
    11221 aaccgtggcg tcttccggtg cttcattcca attcatgcag atattctggc gcttctgaat
    11281 ctccaaccag tggtcactgg aacggtcgat tctgaaaaac aaagaacctg aacgttgtgg
    11341 caatcccggc atcggcgtta cgctgtgttc cagcggaatc ccgggaaggg ctctcttgat
    11401 cagcgtcggc atgtattcct cgctgctgac tttcgctacg ttctgcaaca tagggagcac
    11461 ttcttcccta tcctgggaag ttccgaccac aaggtagaac acgttgcgac tatcgaaagt
    11521 gtccagaggt atcgatgcct tgaaatttcc gtcttcgcga acgagacgaa taacgttttc
    11581 cgcacctaag gtaatctcgt tgagcaactc cccgataagt atctgcgcct catcaaaaca
    11641 gccgccgata tcgaaatgat tgtaatctgg catcaggggc gatccgtccc gtaatctgcc
    11701 gagggcgtcg accctgtcgg taaatgtgct gagttcgccc acgagttgcc tgagtaatcc
    11761 ataagcgtac cagggatgga cggcgggcgc ctccgtgacg tggtggagca gcgggacata
    11821 ccggttcaaa gaacggaggg ccagaagata tatcgtgaag ctgaatccca tatccgatga
    11881 ttgggcctcc cgtggaactt tatactcctc gagcatacgg cagcgtgccg tgaccttttc
    11941 gcggatgttt ttcatggtct gagcgaggat gccggaagag gaaaccgata ctgacggagg
    12001 aacaaacctc cgggaaagct tgatgtcatc catctcccgc tccaagcagg caatcgggat
    12061 cagttcataa tcaccggcgc cttcgatttc gttttcccag aatactttca aaagataatt
    12121 aagaaattta atatgtgcag gtggccctcc gtgatggata tccttgagtt cccgtggatt
    12181 gacgtcggag atgaagcggg tggcggcggc agaaacggca tccggtgtgt tcaagacact
    12241 gacattttcc cctctggcgt tccacttgcg taatcccaag tagatattga acggcttttc
    12301 ctcggcccac agacctttga acgagcgggg ttgaaccact gcgttatcgg gaaacgacac
    12361 cagtgtcccg tcctgaaaaa tgaattcgcc ggcggatatt tcaaacatcc tgttattcag
    12421 ggcgggctca aagatgtcca tcttgcagac gccccagaaa tacggctgtc ggtgtgcttg
    12481 aagattatgg agaagggatt gaaaatagag atcgttctgc tgaaaatgct ggggttgtaa
    12541 aaaaagccct tgataccaga aaatcggttg tctctgttcc attatttcac taccacctct
    12601 cgttcatgca gggcgctcgg tcccaaaaac aacttaatgg tcatttgtcc ggcacgggat
    12661 tccgtctttc ggaacccata gatgcctttc ttttctgtaa tcagcggtat gttgaatgac
    12721 cggctcacct tgccgggatc gagggcatag tacccggcga caatcccgac ccatctggcg
    12781 ccttcggcgc gattcagaac tataatcttt tcttcggctg gctggacgat gaagttcttc
    12841 acgccgacca cgctgggatc gaaactctga ccctggagaa gcttcttcag cccatcgaca
    12901 tcttgagtca gattattgaa ggtattgata ttactcgtct ggtaaaccga tagaatgagt
    12961 gtgtggggtt cgttttcaaa gacattcaac atactgtcgg cggtatattg aatcttaatt
    13021 cccccgtgcg caaaagacca gacggacttt cccaacgaca tctcagccgt gtccgttgcg
    13081 gtttttgttc cgcaagaagt gaaaagaaaa aatgaaatga tcgcaaagag ggcaaaaagc
    13141 gtgacgacgc tcgaggaacc gggctttccg gcaagtggta tatccccgta ccgcaccttt
    13201 ccacgctcag cgggagagat ggatcgaccg ttcatgtttt taggagaggt cacgtaatca
    13261 gttcatcctg gaaaataccg tttgtaagcc tgagacaaat attactacgg ttttttgaaa
    13321 gctgaataga tttgtggagt tgtagttgtc tttgcccgga gaggcgttgt aattccgtat
    13381 tctgaagcac cgttggattg ttcaggtgga cgaggtgacg aggtactcca atgatgaagt
    13441 tgtctggcgg tgtttctgcg aatccaacac ctttcaggaa ggtcaaatta cggaagtgac
    13501 atctctatcg aggttataaa ggcggatcgg tgcaaagggc gtgaaacaaa aagcagatag
    13561 aagcggtgtg cgggctcaat cagttggttt ttttcggcga gagaatcgct gatcagtcag
    13621 atgaaacaga tcctctgcgg gcaatcatcc cggaagtggt tctatttttg gcaagagtca
    13681 tcccgatttc aaaaagggca ggaacttcat cccgagagct atgagtcctg gttccgaaag
    13741 gcctaagaca ataggccaag atcaagcctg caatcagcca catcggtgag cgattatcgg
    13801 atgaatcgat gccgatacaa gggcaccata aatgacacga tcaatgttgt gttgtatgat
    13861 ccactttggc ctggaacatg aaaaagatcc ttcagttcca taggcctgaa ggaagaaaaa
    13921 taaaccttga ggcaaataac acaatatgca taggggaaag caacccacga taccgatcct
    13981 tatccacatc tatcttcagt tttcaaagaa cgctgcgacc taaaactttt ttaaagtatc
    14041 ctatttttca aagcggactg atgagacctt agcgcgaagt ctctctttga gaggacgtcc
    14101 cgcagactcc ccgtcgaacc tctttacgaa tacttataaa attcatatgc gaccgttttt
    14161 gaagctttaa catagttaaa gcaagagctt ctgtacgaga catccgggaa aggccttttc
    14221 cttgagggga aaatcctata aattcctgaa caggaagcaa cttccaaaac acttttttta
    14281 acacatatca cactagttac agagttcaca agaaagaatt gatgagacct cagggactcg
    14341 tcaaagtggt gagggggctt tgtatggagt acggttcagg gttggtagac gagggttgag
    14401 gaggttgtgt gcagggcaga tggg
//
LOCUS       DAOTMX010000003        13065 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_4602_length_13065_cov_4.151191, whole genome shotgun sequence.
ACCESSION   DAOTMX010000003 DAOTMX010000000
VERSION     DAOTMX010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 13065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13065
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4602_length_13065_cov_4.151191"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="P5573_00185"
     CDS             <1..202
                     /locus_tag="P5573_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT26515.1"
                     /translation="FKVLEDHFRLDFVAPYHKTVFLYDGQSLVRVTEQGLGNNHEGDR
                     AMINEWARLLQLTGWFEWKRLE"
     gene            complement(212..715)
                     /locus_tag="P5573_00190"
     CDS             complement(212..715)
                     /locus_tag="P5573_00190"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HRT26516.1"
                     /translation="MAFEIERRFLVKNDDWKRRASVGTTYRQGYIFTGKNGTLRVRVA
                     GDRGSLTLKGLLEGITRHEYEYEIPLAEAEEIMSVFCERTPVEKVRYTVQEAGTRWEV
                     DVFSGVNHGLVIAEVELSEEGESIELPGWAGEEVTGDARYLNVNLYLHPVGEWPAGDR
                     PLPPERR"
     gene            861..1739
                     /locus_tag="P5573_00195"
     CDS             861..1739
                     /locus_tag="P5573_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT26517.1"
                     /translation="MFPLMEGCGFVHLCQPDAGKSCGACCGLYNYADSSEEALTERLR
                     RRTRLFREKVESEEDLPAFSERIKSIEDGTKRYDVIYCCEYLGFLDEDERRVGCLLHP
                     LQNGGRDLRGVSFYGRELCDGHFCPSYHYISEVEKRSLIKILDSWYLYGLCVTDIDLV
                     KEYFRLVSDGIGEMPPPGCFERPALRDAAGRFFSLKTTWPFRSSSVNRFGKYYFDGSQ
                     YMIRPIDYERLGVDKSVFDMIFLSLSSEFAGGEEVKEAEDIIRSLIDDFIRLYRREGG
                     SFPVEEEMKTETGEHG"
     gene            1869..2393
                     /gene="hpt"
                     /locus_tag="P5573_00200"
     CDS             1869..2393
                     /gene="hpt"
                     /locus_tag="P5573_00200"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HRT26518.1"
                     /translation="MEVENKKVLYPRDVISRRVAEVAEEISRDYRGREPVLIGVLKGA
                     FIFMADLVRGLKVPCVIDFVRVASYGSGSTSSGNVVIKKDIETDITGKDVLIIEDIVD
                     TGITMRFLVEKLKERNPRSLKVCAFIDKRGRREVEFEADYVGFTMEDGFVVGYGLDFD
                     EKSRFHPEVYVVDE"
     gene            2386..3201
                     /locus_tag="P5573_00205"
     CDS             2386..3201
                     /locus_tag="P5573_00205"
                     /inference="COORDINATES: protein motif:HMM:NF023334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3426 domain-containing protein"
                     /protein_id="HRT26519.1"
                     /translation="MNDKKMIIRCTNCGTRFWFNEALVQGEGVWVRCSRCENVFFQEN
                     PSAPPPAEPVEDEVADRDVGQVTEGVPGGDVMSESENEAGPTASGKGILKKTAVAVFL
                     LLLVLGVVVWLMPEVLTAALPGGSYIARYFGIGSPGHPAGPGIDLVDVRDRYVKNWLA
                     GDMMVIGGTAVNRNAYPVSKLRVKGTLLDAEGRVVAEAHSYCGNTPTDEDLTKLTVKE
                     ILDKLSLEAGSDAPNMNIAPGGRTPFITVFVDPPKTAVEYIVEVVSVEASKKK"
     gene            3214..4212
                     /locus_tag="P5573_00210"
     CDS             3214..4212
                     /locus_tag="P5573_00210"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel protein"
                     /protein_id="HRT26520.1"
                     /translation="MRPTDKLRIAILGLVIMIFIGTVSYNAVEGWDLLDALYATIVTL
                     STVGYGDFFPRSEAGKIFTVVFVIFGVGIMFYTVVLMAETVVDVRLRSILGRDKFGKV
                     ISRMSNHIIICGCGRIGHLIARELAAAKRDFVVIENNPETVEKIQEEGFICCKGDATS
                     DKVLLEAGIKRARAIVCVLPTDAENLYVILTAKELNPEIFILSRAEEEESEHRLVRAG
                     ADRVMSPYMLGGIRMAMAILRPAMLDFIEITTRRQSLELQMEELEICEGSPVIGKTLE
                     ESDIRHSFGLIIVAVKKESGRMIFNPMAHYVIEKGDKLIALGEEENVRRFSQVCIG"
     gene            complement(4181..4822)
                     /locus_tag="P5573_00215"
     CDS             complement(4181..4822)
                     /locus_tag="P5573_00215"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HRT26521.1"
                     /translation="MPILIRTDPTNLDKKALLEAVAVLKGGGVVAYPTETFYGLGADA
                     SDEKAVERVYALKGREFNKPLPIIIGDREIVSRLVEEIPETARKLMDAFWPGGLTIVF
                     KASPFIVPRLTGGTGKIGIRLSSNPIASKLSALLSRPLTATSANLSGEKESVSAREVL
                     EILGDRVDAVIDGGSTPGGKGSTIVDATTDPPTVLRAGVIPPSLIRYRLEKNA"
     gene            complement(4810..5319)
                     /gene="purE"
                     /locus_tag="P5573_00220"
     CDS             complement(4810..5319)
                     /gene="purE"
                     /locus_tag="P5573_00220"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HRT26522.1"
                     /translation="MKQKRGVLVSIAMGSDSDLPVLSEAITILKEFDVTYEAVLTSAH
                     RSPERTAAYAEGAAERGVKVIIAGAGAAAHLAGVIASRTTIPVIGVPIDSTPLAGLDA
                     LLSTVQMPGGVPVASMAVGKAGAKNAALMAVRILALGDDALREKLAAYAAKMARDVEK
                     KQEKVDCLS"
     gene            complement(5360..6928)
                     /gene="purH"
                     /locus_tag="P5573_00225"
     CDS             complement(5360..6928)
                     /gene="purH"
                     /locus_tag="P5573_00225"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HRT26523.1"
                     /translation="MRLNEIKRALISVTDKAGIVEFTAGLRAFGVEILSTGGTAALLK
                     KNGVEVREVSEYTGFPEMMDGRLKTLHPKIHGGILAQRDKEEHVTAASSQGIEMIDIV
                     VVNLYAFEDTVARPGCTLEEAVENIDIGGPAMLRAAAKNYPFVTVVTDPQDYRLILEE
                     MKATGGKVSEETNFSLAVKAFHLTARYDAAISNYLGRIRPDGAREDFPDTLTFQFVKA
                     LDLRYGENPHQKASFYKEKDATPSTIANARQLQGKELSYNNIMDSDAAWQMVCDFDKP
                     AAVIVKHANPCGAATSTEDILSAYKKALQTDPLSAFGGIVAFNKPVDRQTAEELAKTF
                     LEVIIAPGYDREALEVLGTKKNLRLLEVAPCTEGGFPGYDFRRVVGGLLVQERDVNDG
                     NVRNGKVVTERTPTDDEFEALNFAWRVVKYVKSNAIVIATKDQLIGVGAGQMSRIDSL
                     KIALMKANFPTKGAVLASDAFFPFRDVVDTAAAAGITAMVQPGGSIRDEESIQAANEH
                     GMAMVFTGRRHFKH"
     gene            complement(6943..8271)
                     /gene="purD"
                     /locus_tag="P5573_00230"
     CDS             complement(6943..8271)
                     /gene="purD"
                     /locus_tag="P5573_00230"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HRT26524.1"
                     /translation="MTRVLLVGNGAREHAIAEAVVRSKHQPRLFSYMRHNNPGIAGLS
                     ERVMLGSYDDLESLCRFARENRIDFAVIGPEDPLNNGAVDALKAAGIPAVGPTKKLAQ
                     LETSKSFTRNLLARYGIPGNPRFRVFTSLDGVEEFLKSLDGAVVKPDGLTGGKGVLVQ
                     GDHFQTVEEALKCCGEILAEHPAVIIDEKLEGEEFSLQCLSDGKTVVATPPVQDHKRR
                     FIDDKGPNTGGMGSYSCEDHSLPFLTRKDVEDGLAITQKVAEAIYKETGEYYRGVMYA
                     GFMVTKDGLKLLEYNARFGDPEAMNILPILKTDFVDLCRAVIDGTLGESAVEFEKKAT
                     VCKYIVPRGYGLPKDHPDADSTSARIEVGDVGPARLYYASVDQREDGLYMTTSRAIAV
                     VGIADSLDEAETIAENAATAIKGPVSHRPDIGTGPLIEKRIRHMKKLRGE"
     gene            8498..9760
                     /locus_tag="P5573_00235"
     CDS             8498..9760
                     /locus_tag="P5573_00235"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRT26525.1"
                     /translation="MDPYLTFKLVVLFFLFLASGFFASAEASLFSLTPLHLHKVREEE
                     SPRLSTIQELLRYPRRLLITIIACNESVNVMISSLTAVIFIKLFGDQGKWTAIAVLTP
                     LLLVFCEAVPKTVAKINPIAISQAVSPLLVLFFRLLSPVSWVLDKISGWLTGPFRLGE
                     PSGKDELTEGELRVLVDAGHEEGVLQKPHRDLIHRVFELADTTVEEIMTPRIDMFCLP
                     VSLTLDEMKKEILKNGYSRVPVYGMDRDDILGILYAKDLLLAMASGRRETDVRALLRK
                     PYFVPMEKEADSLLKDFQVRKIHMAIVVDEYGGVAGLVTMEDILESLFGDIYDERDIK
                     ERYYRVLDGQTMIASGRMPIEEFNALTGMNIPLEDFDTVGGFVLHLFGRLPSRGEEVR
                     YDGMVFRVERVRGASIWRVRVSREEVAK"
     gene            9757..11037
                     /locus_tag="P5573_00240"
     CDS             9757..11037
                     /locus_tag="P5573_00240"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRT26526.1"
                     /translation="MTDHLLSILMVIFFLCLEGLFSGGEIALVASDINKVKQRAAAGS
                     GSAAVVLRLLEKPEWFLATTVAGTDFCIIVNTALATSVFVSLFGSTEGGVVAVAVMIP
                     VILLAAELIPKSIFQQRPESSAAKVAYFILLASWVLYPLVLAVAWISRAGIGLLTGGR
                     AAYAPYITKEGLKHLLREQAGKGDIEPQEKEMVRRVFDFSDATVGQIMVPLSNVAALK
                     ETAQVGDAVTLIREKGFSRIPVYRESIFDITGILHSFDLLRALPEGEQKPVADYVRSA
                     VFFVPETKSAYELFLEMQRRGEVMAVVVDEYGGAVGIITTEDLLEEVVGEIEDEYDKG
                     ERPYLKVGPGRYLFKAQTSLDCVREITRLEIPEGDYETLGGFILDRLGHIPRRRESVR
                     EGDVLFVVEDADAKSVKEVLIVLPDKKDGSESIA"
     gene            11015..11782
                     /locus_tag="P5573_00245"
     CDS             11015..11782
                     /locus_tag="P5573_00245"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HRT26527.1"
                     /translation="MAQNLSPKLATIILAAGKGTRMKSDLAKVLHPIDGKPMLSFPVA
                     VAREVDSREVVVIVGHQAELIEEVMKDEGLVFVMQKEQLGTGHAVLQARDHFRGFEGT
                     ILILCGDVPLLRSRTVRDLIAFHRAEKAAVTVMTTVLEDPAGYGRVVKDGGGGIVKIV
                     EARDATEEEKEIREINTGIYCAEAPFLFEAVGEIDNDNAQGEFYLTDIVAVTLRKGKK
                     ACSFVAADSREVMGINTQDELEEAERILRLTDQESRA"
     gene            12244..12390
                     /locus_tag="P5573_00250"
     CDS             12244..12390
                     /locus_tag="P5573_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT26528.1"
                     /translation="MIPRYWCLSCDEGVSGLEELLDAGEAICHAFIVGELACGNLRNR
                     PEIL"
     gene            complement(12664..>13065)
                     /locus_tag="P5573_00255"
     CDS             complement(12664..>13065)
                     /locus_tag="P5573_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HRT26529.1"
                     /translation="ERKQPVRFTRSRAYHKEDNAHVEQKNWTQVRQWVGYDRLDNPMV
                     VPLLNDLYRNEWRLFHNFFLPSVKLIDKERIGSKTLKRYDPPKTPYQRTMESDYIPNA
                     VKKDLSKQLEKLNPFLLRKNMETKLKKIFSL"
BASE COUNT         2667 a         3519 c         3936 g         2943 t
ORIGIN      
        1 cttcaaggtc ctggaggacc acttcaggct ggattttgtt gccccctacc acaagacggt
       61 tttcctgtac gacggccagt ctctcgtccg ggtgaccgag cagggattag gcaacaacca
      121 tgaaggggac cgggcgatga tcaacgaatg ggcgagactc ctccagctca cggggtggtt
      181 cgagtggaag cgcctagagt gacggaaggg ttcaccgcct ttctggcgga agcggtcggt
      241 cgccggcagg ccattctcca acggggtgga ggtataggtt gacgttgaga taccgggcgt
      301 cgccggtgac ctcttcgccc gcccatccgg ggagttcgat ggactcgcct tcttccgaga
      361 gctctacttc cgcgatgacc aggccgtgat tcacccccga aaagacgtct acctcccacc
      421 tcgtccccgc ttcttgcacg gtataacgga ccttttcgac cggggttcgc tcacagaaga
      481 ccgacatgat ctcttcggcc tctgcgaggg gaatttcata ttcgtactcg tgccgggtga
      541 tgccttcgag aaggcccttg agggtaaggg atcccctgtc accggcgacc ctcaccctca
      601 gggtgccgtt ttttcccgta aagatatagc cctgccggta cgtcgtaccg acggaggccc
      661 gccgtttcca gtcgtcattc ttcacgagaa atctgcgctc tatttcgaag gccacggaga
      721 aaccctgagt ttttagggtc gagcttctcg gcccaaagga ttcggaactt agaagttcta
      781 tagccatgtt tccggggatt ttcaataagg agtttgaacc atccggcctc tttctgttgt
      841 cccccggaaa aagttctgtt atgttccctc tcatggaggg atgcggtttt gtacatctct
      901 gtcaaccgga cgctggaaag tcctgcgggg cctgctgcgg tctttataat tatgccgaca
      961 gctcggaaga ggcactgacg gaaaggttga ggaggcggac gcggcttttt cgggaaaagg
     1021 tcgagagtga ggaagacctg cccgcctttt cggaaaggat aaagagcatc gaggacggga
     1081 cgaaacgcta cgacgtgatt tactgttgcg aatacctcgg ttttcttgac gaagacgaaa
     1141 ggcgcgtcgg ctgcctgctc catcctctcc agaacggggg cagggacctg cgcggggtgt
     1201 cattctacgg acgggagctc tgcgacggcc atttctgccc gagctaccac tacatatcgg
     1261 aggtcgaaaa acggtccctc atcaaaatcc tggacagttg gtacctctac ggcctctgcg
     1321 tcaccgacat cgacctcgtg aaagagtatt tccgcctcgt cagcgacggc ataggtgaga
     1381 tgcctcctcc gggctgtttc gagcgcccgg ccctcaggga tgcagccgga cgttttttct
     1441 ccctcaagac aacctggccc ttccgttcgt cctcggtcaa ccgcttcggt aaatactatt
     1501 tcgacggctc ccagtatatg atccgcccca tcgactatga gcgcctcggt gtggataaat
     1561 ccgtgttcga catgatattc ttgagtcttt cctccgagtt tgcgggcggt gaggaggtga
     1621 aagaagcgga agacatcatc cggtctctga tagatgactt tatccgtcta taccgcaggg
     1681 aaggcggctc attccccgtt gaagaggaaa tgaagaccga gaccggcgaa catggataag
     1741 ggcacgcagc cggcatagca ttttcatgct gaattttctg actttttttg ctaatcactg
     1801 actgttccgt atgcctagtt tgtgtgcgtt tcagggcttt tcagataggt tttttgggag
     1861 ggcgggagat ggaagtcgag aacaagaaag tgctctatcc cagggatgta atcagccgcc
     1921 gggttgcgga ggtcgccgaa gagatctccc gggattaccg cggcagggag ccggtcctga
     1981 taggagtact caaaggggcc tttatcttca tggcagacct tgtccggggc ctcaaggtcc
     2041 cctgtgtcat cgactttgtg agggtagcaa gttacggttc cgggtcgacg agctcgggca
     2101 acgttgtcat aaaaaaagac atcgaaacgg acataacggg gaaagacgtc ttgataatcg
     2161 aagacatcgt cgatactggc atcaccatgc gtttcttggt ggagaagctc aaggagcgga
     2221 acccgaggtc gttgaaggtc tgtgccttta tcgacaagcg gggccgtagg gaagtcgagt
     2281 tcgaggccga ctacgtgggt tttacgatgg aggatgggtt cgtcgtgggt tatgggctcg
     2341 acttcgacga aaaatcacgc ttccatccgg aggtgtacgt tgtcgatgaa tgataaaaag
     2401 atgataatcc ggtgcacgaa ctgcgggacg cgtttttggt tcaatgaggc gcttgtccag
     2461 ggggaagggg tttgggtccg gtgcagccgg tgcgagaatg tcttcttcca ggagaacccc
     2521 tccgcaccgc ctccagcaga gccagtggaa gatgaagtcg ccgaccgcga cgtcggtcaa
     2581 gtgaccgagg gtgtccccgg tggagacgtg atgtcggaat cggaaaatga ggcggggccg
     2641 acggcgagtg gcaaaggcat actgaagaaa accgccgtcg ccgtcttttt gctgttgctc
     2701 gtcctgggag tggtcgtttg gttgatgccg gaggtgctga cagcggcctt gcccggtggt
     2761 tcttacatcg cccgttactt tgggattggg tcgccgggac acccggccgg cccaggaata
     2821 gatttggtcg acgtacggga caggtacgtc aagaactggt tggccgggga tatgatggtc
     2881 atcggcggca cggcggtaaa caggaacgcc tatcccgtgt cgaaactgcg cgtaaagggg
     2941 acgctcctgg atgcggaggg gagggtcgtc gccgaggccc actcttattg cggcaatacc
     3001 ccgaccgacg aggacctcac gaaactgacc gtgaaagaaa tcttggataa actgtcgctt
     3061 gaggcgggca gcgacgctcc caacatgaac atcgcccccg ggggtcggac cccctttatc
     3121 accgtgtttg tagacccgcc aaaaaccgcc gtcgagtaca tcgtggaggt tgtgtctgtc
     3181 gaggcgtcga agaaaaaatg aacggaggcc ggcgtgcgcc cgacagataa attgaggatc
     3241 gccatcctcg gccttgtaat aatgatattt atcggaacgg tcagttataa cgccgtcgaa
     3301 ggttgggatc tgctcgacgc cctgtatgcg actatcgtga cgctttcgac cgtcggctac
     3361 ggggattttt tcccccggtc cgaggcgggc aagatattca cggtcgtgtt cgtgatcttc
     3421 ggcgtcggca tcatgttcta taccgttgtt ctcatggctg agaccgtggt cgatgtgcgt
     3481 ctcaggtcga ttttagggag ggataaattc gggaaggtga tcagccggat gagcaatcac
     3541 attatcatct gcgggtgcgg tcggatcggc catctcatcg cgagagagct ggcggctgcg
     3601 aaacgggact tcgtcgtcat tgaaaacaac ccggaaacgg tcgagaagat tcaagaggaa
     3661 ggctttattt gctgtaaggg ggatgcgacg agtgacaagg tgcttttaga ggccggcata
     3721 aagcgggcgc gggcaatagt ctgtgtgctt ccgaccgacg cggagaacct ctatgtaatc
     3781 ctgacggcca aggagctcaa cccggagata ttcattcttt cccgtgccga agaggaagag
     3841 tcggaacacc ggcttgttcg tgccggggcc gaccgcgtca tgtcacccta tatgctgggc
     3901 ggcatcagga tggcgatggc gattctcagg ccggcgatgc tggacttcat cgaaatcacg
     3961 acgagaaggc agagcctcga gctgcagatg gaggaactgg agatatgcga aggttcgccg
     4021 gtgatcggga agaccctgga ggaatcggac atccgccata gcttcggcct catcatcgtg
     4081 gccgttaaga aggaatcggg caggatgatc ttcaatccca tggcccatta cgtgatcgag
     4141 aaaggcgaca aactgatcgc cctgggcgag gaggagaacg tcaggcgttt ttctcaagtc
     4201 tgtatcggat gagggaaggg gggataacgc cggcccggag aacggtgggc gggtcggtgg
     4261 tggcgtcgac gatggtagac cccttgcccc cgggcgtaga accgccgtcg atcactgcat
     4321 ccacccgatc cccgagaatc tctaaaacct cccgagctga gacactttcc ttttccccgg
     4381 agaggttcgc gctcgtcgcc gtgaggggcc gggaaaggag cgccgaaagc tttgatgcga
     4441 tggggttgct ggagaggcgg atgccgatct tgccggtgcc ccctgtcagt ctcggaacga
     4501 tgaacggcga ggccttgaag acgatcgtga gaccccccgg ccagaaggcg tccatcagtt
     4561 ttcgggccgt ctccggaatc tcttcgacca gcctgctgac gatttcccgg tctccgatga
     4621 tgatcggcag gggtttgttg aactccctgc ctttgagggc gtaaacccgt tccaccgcct
     4681 tttcgtcgct cgcgtcggcg cccaacccgt agaaggtttc cgtcgggtag gcgaccacac
     4741 cgccgccctt caggacggcg acggcctcca ggagggcctt tttatcgagg tttgtcgggt
     4801 ctgtcctgat caggataggc aatcgacctt ttcctgtttt ttttcgacgt cccgggccat
     4861 ctttgcggcg taggccgcca atttttcccg cagggcgtcg tcgccgaggg cgaggatcct
     4921 gactgccata agggccgcgt tctttgcccc cgccttcccc accgccatcg aggccaccgg
     4981 tacgcccccc ggcatctgga cggtcgagag cagggcgtcg agtcccgcca gcggcgtcga
     5041 gtcgatcggc acgccgatga cgggtatggt ggtccgggaa gcgattacgc cggcaagatg
     5101 cgccgcggcc cctgccccgg cgatgatgac ttttactccc ctttccgccg cgccctcggc
     5161 gtaggccgcc gtcctctcgg gcgagcggtg ggccgacgta agcacggcct cgtaagtaac
     5221 gtcgaattcc ttgaggatgg ttatggcttc actgaggacc ggcaggtcgg aatcactccc
     5281 catggcgatg ctcaccagga cacccctttt ttgtttcatg ccttacccca ttttgagacg
     5341 gagattctta tgacgtgcat cagtgtttga agtgcctcct ccccgtgaac accattgcca
     5401 tgccgtgctc gttcgccgcc tgtatggatt cttcgtcccg gatggaacca ccaggctgga
     5461 ccatggccgt tatgccggcg gccgccgccg tgtccacgac gtcgcggaag gggaagaatg
     5521 catcggaggc aaggacggcg cctttcgtgg ggaagttggc cttcatgagg gctatcttca
     5581 gggaatcgat gcggctcatc tggcccgccc ccacaccgat gagttggtcc ttggtggcga
     5641 tcacgatggc attcgacttg acgtacttca cgacacgcca ggcaaaattc aaggcctcga
     5701 attcatcgtc agtgggggtg cgctcggtaa ccaccttacc gtttctgaca ttcccgtcgt
     5761 ttacgtcgcg ctcctgaacg agaagccccc ccaccactcg tctgaagtcg taccccggga
     5821 agcccccctc cgtgcaggga gcgacctcca ggaggcggag gttcttcttg gtccccagga
     5881 cctcgagggc ctcccggtca taaccggggg cgatgatgac ttccaggaag gtcttggcga
     5941 gctcctccgc cgtttgtctg tccaccggct tgttgaaggc gacgatgccg ccgaaggcgg
     6001 agagggggtc tgtctgaagg gcctttttgt aggccgagag gatgtcttcc gtcgaggtcg
     6061 ccgcgccgca ggggttggcg tgtttgacga tgaccgccgc cggtttgtcg aagtcgcaga
     6121 ccatctgcca ggcggcgtcg ctgtccatta tgttgttgta agacaattct ttgccctgga
     6181 gctggcgggc gttggcgatg gtcgatgggg tcgcgtcctt ttctttgtag aaagacgcct
     6241 tctggtgggg gttttccccg tacctcaggt ccagggcctt gacgaactgg aaagtcaagg
     6301 tgtcggggaa gtcctcccga gccccgtccg gccgaatcct gccgaggtag ttggagatgg
     6361 cggcgtcgta acgggccgtg aggtggaatg ccttgacggc caaagagaaa ttggtttctt
     6421 ccgagacctt tcctcccgtc gccttcatct cctcgaggat gaggcggtag tcctgcgggt
     6481 ccgtgaccac ggtgacaaag gggtagttct tcgccgccgc ccggagcatg gccggtccgc
     6541 cgatgtcaat gttctcgacc gcctcctcca gggtgcaccc cggcctcgcc acggtgtcct
     6601 cgaaggcata gaggttgacg accactatgt cgatcatctc tatcccctga gaactggccg
     6661 ccgtcacgtg ttcctctttg tccctctggg cgaggatgcc gccgtggatc ttcgggtgga
     6721 gggtcttgag cctcccgtcc atcatctccg ggaagcccgt gtattcggag acctcccgca
     6781 cctcgacgcc gttctttttc agcagggccg ccgtcccgcc tgtggagagg atttcgacac
     6841 cgaaggcccg cagcccggcg gtgaattcca cgatgcccgc cttgtcggtc acgctgatca
     6901 aggcccgctt gatttcattg agtctcatct gtttcctccc cctcattcac ctcttaactt
     6961 tttcatgtgc cgtattctct tttcgatcag gggccccgtg cctatatccg gcctgtggga
     7021 tacggggcct ttgattgccg tggccgcgtt ctcggcgata gtctccgcct cgtcgagcga
     7081 gtcggcgatc cccaccacgg cgatggcccg cgacgtcgtc atgtaaagac cgtcctcgcg
     7141 ctggtccacg gaggcgtaat aaagcctcgc cggccccaca tctcccacct caatcctggc
     7201 cgaggtcgaa tctgcgtcgg gatggtcctt ggggagaccg taaccccggg ggacgatgta
     7261 cttgcacacc gtcgcctttt tctcgaattc gacggccgat tcgcccagcg tgccgtcgat
     7321 gaccgcccgg caaaggtcaa cgaaatcggt cttgaggatg ggcaggatgt tcatcgcctc
     7381 ggggtcgccg aaccgggcgt tgtactcgag gagtttcagt ccgtctttcg tgaccatgaa
     7441 cccggcgtac atcactcccc tgtagtattc acccgtctcc ttgtatatcg cctcggctac
     7501 tttttgcgtg atggcgaggc cgtcctcgac gtctttccgc gtcaagaagg gaagggaatg
     7561 gtcctcgcaa gagtaagacc ccatcccgcc cgtgttgggc cctttgtcat cgataaaacg
     7621 cctcttgtgg tcctggacgg gaggcgtcgc cacaaccgtc tttccatcgg agaggcactg
     7681 aagcgagaac tcctctcctt ccagcttctc atcgatgatc acagcggggt gctccgcgag
     7741 tatctccccg cagcatttca atgcctcctc gaccgtctgg aaatggtcgc cctgaaccaa
     7801 gactcccttg ccgcccgtga ggccgtcggg tttcacgacg gcgccgtcca ggctcttcag
     7861 gaactcttcg accccgtcga gggaggtgaa gacgcggaac ctcggattcc ccgggatgcc
     7921 gtaccgggca agcagatttc gggtgaaaga cttcgacgtc tcgagctgcg ccagcttctt
     7981 cgtcggaccc acggccggta tcccggcggc cttcaaggca tcgacagcac cattgttcaa
     8041 agggtcctcc ggcccgatga cggcgaagtc tatgcggttc tccctggcga acctgcagag
     8101 agactcaagg tcgtcatagc tgcccagcat aacccgctcg gacaggcccg cgatcccggg
     8161 gttgttgtgc ctcatgtagg aaaatagtct cggctgatgt ttcgaccgga cgaccgcctc
     8221 ggcgattgcg tgctccctgg cgccgtttcc gaccaaaaga acgcgtgtca ttcttcctcc
     8281 tcgtgggcct cggccttcgg ttgaggtttg ggcatcagac acacaaccgg caggggatgc
     8341 ccagtgcttg tgagcttgcg actatatcca aaacggggta gggagtcaat aaatcaaagc
     8401 tgaatacttg acatttcaag cgaatgaggg taccaatctc ccgagcgggt aatcgtccat
     8461 gaccacatct tcaagcgacc gatagagcag gatccccttg gacccttatc tgacattcaa
     8521 acttgtcgta ctcttctttc tctttttagc ctccggtttt tttgcttctg cggaggcctc
     8581 acttttctct ctgacgcctc ttcatctgca caaggtgagg gaagaggagt caccgcgcct
     8641 ctccacaatc caagaactcc ttcgttatcc ccggcgtctg ttgataacga tcatcgcgtg
     8701 caacgaatct gtcaacgtaa tgatttcttc gctgacagca gtgattttta taaaactctt
     8761 cggagatcaa gggaaatgga ccgccatcgc cgtcctgact cccctgctcc ttgtcttctg
     8821 cgaggcagtt ccgaagactg ttgccaagat aaacccgatc gcgatctcgc aggccgtttc
     8881 gcctttgttg gtattgtttt tccgcttgtt gtcccccgtc agctgggtgc tggataagat
     8941 atcgggctgg ctgacgggtc ccttcaggct gggtgagccg tcgggaaagg atgagctgac
     9001 ggaaggggag ttgcgggtct tggttgacgc aggtcacgaa gaaggagtgc tgcagaagcc
     9061 ccatcgcgac ttgattcacc gggtcttcga gctggcggac acgactgtag aggagataat
     9121 gacgccgagg atcgatatgt tctgcctgcc cgtttcgttg accctcgatg agatgaaaaa
     9181 ggaaatactc aagaacggct actcacgggt gcctgtgtac ggcatggaca gggacgacat
     9241 ccttgggatc ctgtatgcga aggaccttct tctggcgatg gcatcgggac ggcgggaaac
     9301 ggacgtcagg gcgttgctga gaaagcccta ttttgtcccg atggagaagg aagcggacag
     9361 cctcctgaag gactttcagg tgagaaaaat ccatatggcg atcgtggtgg atgaatacgg
     9421 cggcgttgcg gggctggtta ccatggagga catcttggag agtctcttcg gcgacatcta
     9481 cgacgagcgt gacatcaaag aaaggtacta tcgcgtctta gacggtcaaa ccatgattgc
     9541 ctcgggaagg atgccgatcg aagaattcaa cgcccttacg ggcatgaaca tccccttgga
     9601 ggatttcgac acggtgggag gttttgtgct tcatctcttc ggaagacttc cttcccgagg
     9661 cgaagaggtc cgttatgacg gcatggtgtt ccgggtggag cgagtgcgcg gggccagtat
     9721 ctggagagtc cgtgtttccc gcgaagaggt cgcaaaatga ccgaccacct tctgtctatc
     9781 ctcatggtga ttttcttcct gtgtctcgaa ggtctttttt cgggtgggga gatcgctctc
     9841 gtcgcttccg acatcaacaa ggtcaagcag agggcggctg ccggctccgg gtccgccgcc
     9901 gttgtcctac gcctccttga aaaacctgaa tggttcctgg ccactaccgt agcgggaacg
     9961 gatttctgca taatcgtgaa caccgccctg gcgacctcgg tgtttgtttc cctcttcggc
    10021 agtacagaag gaggggtcgt tgccgtggcg gttatgatcc ccgttatcct gctggcggcg
    10081 gagctcattc ccaagagcat atttcagcag aggccggaat catcagcagc aaaggtcgcc
    10141 tacttcatcc tgcttgcgtc ctgggtcctt tacccactgg tgctcgccgt ggcctggatc
    10201 tcccgggcag ggatcggctt gttgacgggg ggaagggccg cctacgctcc ttacatcacg
    10261 aaagagggcc tcaagcatct tctcagagaa caagcgggga aaggtgacat cgaaccccag
    10321 gaaaaagaga tggtcaggag ggtcttcgat ttctcggatg ccacggtggg acagatcatg
    10381 gtgcccctgt cgaacgtggc ggctctgaag gaaaccgccc aggtcggtga tgccgtcacc
    10441 ctcattcgcg aaaagggctt ctcccggata cccgtctacc gggaaagcat ttttgacata
    10501 accgggattc ttcacagctt cgaccttcta agggccttgc cggaagggga gcaaaagccg
    10561 gtagcggact acgtgcggtc ggccgtgttc tttgtccccg aaacgaagtc ggcctatgag
    10621 ttgttcctgg agatgcagcg gcgcggggag gtcatggccg tcgtagtgga tgaatacgga
    10681 ggggccgtgg gcatcatcac caccgaagac cttctggagg aagtggtcgg ggaaatcgag
    10741 gatgagtacg ataaaggcga aaggccatac ctgaaggtcg gcccgggccg ataccttttc
    10801 aaggcccaga cgagcctcga ctgcgtgcgc gaaatcaccc gcctcgagat tcccgaagga
    10861 gattatgaaa ctctgggagg ctttatcctg gaccggctgg ggcacatacc ccggcggaga
    10921 gaaagcgtca gagaagggga cgttcttttc gtcgtagagg acgcggacgc aaagtcggtc
    10981 aaggaggtcc tgatcgtgct gcccgataaa aaagatggct cagaatctat cgcctaaact
    11041 tgctactatc atccttgccg ccggcaaggg aacgaggatg aaatctgatc tggcgaaagt
    11101 ccttcatccg atcgatggaa aacccatgct cagctttccc gttgcagtgg cccgggaagt
    11161 cgactcacgt gaggtcgtcg tcatcgtcgg ccaccaggca gagttgatcg aagaggtgat
    11221 gaaggacgaa gggctggtat tcgtcatgca aaaagagcaa ctgggaaccg gccacgccgt
    11281 cctgcaggcc cgagaccact tccgtggctt tgagggaact atcctgatcc tctgcggaga
    11341 cgtgccgctc ctgcggtcgc ggacggtcag ggacctcatc gcctttcacc gtgcggagaa
    11401 ggccgctgta acggtcatga cgacggtcct cgaggaccca gcgggttacg ggagggtcgt
    11461 gaaggacggt ggaggcggga tagtcaagat cgtggaggcg cgggatgcga cagaagaaga
    11521 aaaggagata cgggagataa acaccggcat ctactgcgcg gaggcaccct ttcttttcga
    11581 ggccgtggga gaaatcgaca acgacaatgc ccagggagag ttctatctga cggacatcgt
    11641 tgccgttacc ctgcgaaaag ggaaaaaggc ctgctctttt gtcgccgcgg actctcggga
    11701 ggtgatgggg atcaacacgc aggacgaact cgaagaggcc gaaaggatcc tccgcctcac
    11761 cgaccaggaa agccgcgctt gacaatccgc cgttcagccg cttgttatct gaaaaatcga
    11821 tgctgaaact ccttgacact catgtattgt taacccactt ttacacatac gacactgcat
    11881 ataaatgata aaaactatga gaacaacatt gaatattgat gacgaactga tcaacaaggc
    11941 ataaaagttg acgcgatcgt aaaaagtcca aaaacgtcat gccggactcg atccggcatc
    12001 cagaacatat tgaatatacc ggattccagc gttcgccgga atgacgaaag tggtaattcg
    12061 cgactttttg cgagccaatc aaagttgaca ggtataaaag agaaaacgtc tttggtgagg
    12121 cttggcctgg aagttttgat cgcgagggaa agcggcaaaa ggtttgctaa attaggggga
    12181 accgagaaag atttggggat gagacctcgc ggaggacaga aggcaaagat agaatgatcc
    12241 tcgatgatac ctcggtattg gtgtctctcc tgcgatgagg gtgtttctgg cctcgaagag
    12301 ttattggatg ccggagaggc catatgtcat gcctttatcg tcggcgaact tgcctgcggg
    12361 aacctcagga ataggcctga aatcctgtag cttcttcaag cactgcctat ggcggcccag
    12421 gcggagcatg aagaggtttt acgattcgaa gacaatcgac tgatgggaaa gggattagga
    12481 tgcatagatg tccacttgag tgcatcggta gcgctttcgg aagcggctat gggaacacga
    12541 aatagcggag aagtttttca ctccattatg agtcttgccc gcgataaatt cccgttgcct
    12601 cataaataaa tgttaccgaa gggtaggctg attgagggat cgttgcctcg tagtagatgt
    12661 tactcagagg gagaatatct ttttgagttt agtttccatg ttttttctta gcaggaaggg
    12721 gttgagtttt tcaagttgtt tggagaggtc ctttttgact gcgttgggga tgtagtcgga
    12781 ctccatggtt ctttggtaag gggtcttggg tggatcgtag cgtttgaggg ttttggagcc
    12841 gatgcgttct ttatcgatga gctttacgga gggcaggaag aagttgtgga agagtctcca
    12901 ttcgttgcgg tagaggtcgt tgaggagtgg aacgaccatg gggttgtcta atcggtcgta
    12961 gccgacccat tgtctgactt gggtccagtt cttctgttcc acatgggcgt tatcttcctt
    13021 atggtaagcc ctgcttcggg tgaaccgcac tggctgtttt cgttc
//
LOCUS       DAOTMX010000004        12773 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_4727_length_12773_cov_3.600016, whole genome shotgun sequence.
ACCESSION   DAOTMX010000004 DAOTMX010000000
VERSION     DAOTMX010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 12773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12773
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4727_length_12773_cov_3.600016"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="P5573_00260"
     CDS             complement(<1..322)
                     /locus_tag="P5573_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT26530.1"
                     /translation="MVWVVRYGLIPALITGFVMSVTEKDGQSEDQSMQRDSLLTRPFA
                     AIGRKTINWVNTLGAATIFLVLAVLMIFRPKQWTKVIEQIYYIGARSTTIIVLVALFT
                     GMVLG"
     gene            complement(404..1471)
                     /locus_tag="P5573_00265"
     CDS             complement(404..1471)
                     /locus_tag="P5573_00265"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002528.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HRT26531.1"
                     /translation="MEDRGEKKLRVGVIMGGMSSEKEVSLESGRNIFAKMDRKKYDPV
                     PIFMDSRGAFWEIPVKLVMRNSTRDVETDLKEEATPIAYEGLKSRVDLVYIGLHGKYG
                     EDGCLQGLLELLGIPYTGSGVLGSALGMDKYVCRRLFAMSGIDVPGTLPVDRRAWDSG
                     DKEALLDRLAKEVGFPCVIKPTREGCSTAVKKVVSKEGVPEALIGAFQWDNTALVEEF
                     IEGMEVTCGVLGNDDPFALTPSEAIPTQDILSLEDKFLYGQGENKTPARVSEHVLKSI
                     QDVAVRAYKTLGLKGYARVDMFVRKDGRVTVLEPNTLPGMTPSTVLFHQAAASGINQT
                     RFIDMVIDYALEAHAAKRGPL"
     gene            complement(1522..2580)
                     /locus_tag="P5573_00270"
     CDS             complement(1522..2580)
                     /locus_tag="P5573_00270"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002528.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HRT26532.1"
                     /translation="MEKLRVGIIFGGMSSEKEVSLNSGRNVYDNMDRELYEPVPIFMD
                     SLGLLWELPWQLISQNTTTDVSERLAEEARRVAYEELKERIDLAFISLHGKYGDDGCI
                     QGLMELLGIPYTGPGVLASALGMDKHLQQKVLEAEGLAVPRSIVIHERDWDVRREELK
                     REVVEAFGFPLVTKPVREGSSVGVKINRSQEDLERGVTEALRWDNSILVEEFLEGIEF
                     SSIVLEGDDGEPVALGLTEIHPQSEFYTYDDKYMPGRCRKFTPPKNISREDSERIKAE
                     VLRAYKALGFRCYGRIDGFFLRGGRILVTDPNSSSGMAPSSFFFEQAAEAGMLPSMII
                     TRLIEIALRVHRDKKGPL"
     gene            complement(2611..4575)
                     /gene="alr"
                     /locus_tag="P5573_00275"
     CDS             complement(2611..4575)
                     /gene="alr"
                     /locus_tag="P5573_00275"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HRT26533.1"
                     /translation="MMAGQGEQRYRSWVEVDLDNYRHNWAEVRRLVGPNVRILQVVKA
                     DAYGHGAIEISNEAMKNGASFLGVANADEGVQLRVGDINAPIVILSPATYSEVKEIIK
                     YNLTPSVSDIHFARELERGLEKAGKRLPVHIEVDTGMGRGGTIYSEALPMIQEILGFP
                     HISVEGIFTHLSSSEVEDDTYNQMQWEHFSRLLSGLDDKGIRIPIRHMSNSGGVLNFP
                     RFNLDMVRPGIMTYGIYPGESTVSKAHLVPVMSFKTSVVLLKDFPGGYGIGYGRTYVT
                     GGPTRIATIPVGYGDGYGVILSNQGEALVRGKRAPVVGRVSMDMCTLDVSHIPECEVG
                     DEVVLLGRQGEGYISANEIAAKAKTISYEVICALGKRAPRVFVNQGKAGSVEPRLRRI
                     FIPDEEKSIARIDGMIRRCFQAKTRSEELGDAIYYEMIETLFGKEDRQLELRTHFRYD
                     VHVKEFSEEEKAVDGSVEDYLKVATNVEYTKTLRNDIFLIGCAMNNRHLAALFADPRC
                     EYRWLLNEQSATFKESDFKVERVSVDGEGIPIIRTEKTDRGFEVWCGGDFLNRKLGSP
                     VRVKIEITTKKLKSSNVFSVFLVYPTRGLEISFNYAGTRIRNVREVSFFSGRRSDPEV
                     VKTEGKSVSIRVGDRDWIFPTSGVIFIWDL"
     gene            complement(4582..6831)
                     /locus_tag="P5573_00280"
     CDS             complement(4582..6831)
                     /locus_tag="P5573_00280"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRT26534.1"
                     /translation="MKDRLKKVVSLSPLKISLVLIVAAVVLYVLDPPFFRFMELKTLD
                     LRILSRGERAPGGETVIAVVDEKSVKELGRWPWPRTTIADLVDVLKSYGAKAVGFDIV
                     FAEPDQYSSLADIQELAREFEKAGIGNPKLTRILREKAARADTDAALAQSISKAGNVT
                     LGYFFHMAKKEAAHLTEREIETGWELVAPFKYQMVQVQGNPDEAVLIHAYAAEPNVRV
                     ISEAADSAGYFNVLPDSDGSIRWAPLAIKFRDDFYPSLDLALVNQYLDYPMTALYVAE
                     FGVAGVQVGDIPISTDESGRILVNYLGRAKTFPHYSIADILKGRLAPELFKDKIVLVG
                     ATAVGIYDLRVTPFSVAFPGVEVHANVIDNILHQDFIQRPGWAGLFDIAAILTLGLVI
                     GVGVPRLRAVAGVFLTLFLLATYITLNGYIFSRLNFWLNLVYPVGTILIVYLAITVYK
                     YVTEEREKKKIRGAFQYYLTPSVINELLKDPSKLKLGGDKKNLTVLFSDIRGFTTISE
                     LLPPEELVQLLNEYLTAMTNIVFKYDGLLDKYMGDAIMAVYGAPIEQPDHAERACRTA
                     LEMLEALGVLHKKWTEEGKPALHIGVGINSGDMVVGNMGSQMRFDYTVMGDNVNLGSR
                     LEGINKEYGTSIVISEFTHREVKEKLVCRELDSVRVKGKKLPVKIYELLGDGADAERW
                     APLVRSFESALEKYRAGLWDEAIEGFRKVLEVRPGDGPSELYIQRCADLKANPPEGVW
                     DGVFTMTKK"
     gene            7021..7096
                     /locus_tag="P5573_00285"
     tRNA            7021..7096
                     /locus_tag="P5573_00285"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7054..7056,aa:Thr,seq:cgt)
     gene            complement(7195..8178)
                     /locus_tag="P5573_00290"
     CDS             complement(7195..8178)
                     /locus_tag="P5573_00290"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HRT26535.1"
                     /translation="MPANLPPEYFEAEKKYKQAATLAEKLAALEDLIASVPKHKGTDK
                     LRADLRRRASQLNKEALALKKSKKGGRDSLYNIQREGAAQLALVGFPNTGKSSLLACL
                     TNAQPVIADYPLSTVLPLPGMMPFEDIHIQLVDLPPIGNDSTDGWVSGILRTTDALLL
                     VLDLGDEPDTQAALLLEQIERWNIHVTGRGLDGRPGLYKRALIVANKKDLPGADEGVE
                     ALKRKWGATLPVITVSVHRKENLEELKRAIFDVSGIIRVYSKPPGKEPDYSLPFTIRS
                     GSTVIELAERIHKDFATKLKYARIWGSARFEGQKVEKNHVLRDGDVVELHV"
     gene            complement(8260..8625)
                     /locus_tag="P5573_00295"
     CDS             complement(8260..8625)
                     /locus_tag="P5573_00295"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRT26536.1"
                     /translation="MVNAFLLVLLGLFAGIMSGLIGLGGGIIIVPALVFFFGMSQHLA
                     QGTTLALLVPPVGILAALTYYHQGYVDLKAAGLICAGLFTGGWLGAKIAVSLSDVTLG
                     RIFGVILFLVSLKMMFAKS"
     gene            complement(8748..10175)
                     /locus_tag="P5573_00300"
     CDS             complement(8748..10175)
                     /locus_tag="P5573_00300"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRT26537.1"
                     /translation="MTARFLLQLLLRPFWKVRNIFHGFESGTLNLKPGTGLTVTFIAA
                     SITVAAPAYAFPPVERFVLPNKLKVIVAEEHSLPFATLQLLVDSGSRKDPPGKEGLAR
                     LTAQGLLLGTEKRTAEAINRELDFLGASLGAAAGPDYAVLSLRVLKKDLDRGFDLFMD
                     VLTRPVFPADEVGREVEKTLAAIQSEEDQPDMVAEKAFQKELYLSGYGHPVEGTKESL
                     PAVARGDVERFYRENYCPNNAILVVAGDVTVAEVRASLVPLLDAWKAGDGVAVPFKSA
                     FAEGPKTVKINRSITQANIVIGHAGVSRDNPDYYAVVLMNYILGGGGFSSRLFDEIRV
                     KRGLAYSVGSFFDARRYPGSFQVSIQTKNSSAREAIRIVLDQMKLIQASPVKVKELDG
                     AKKYLTGSFPMRMDTQAKLAGFLAQVEYFGLGLDYPSKYPSLILAVAREKIQDVAKRY
                     LYPDKCIVVIVADLKEAGLDAPGLP"
     gene            complement(10250..11689)
                     /locus_tag="P5573_00305"
     CDS             complement(10250..11689)
                     /locus_tag="P5573_00305"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRT26538.1"
                     /translation="MLLPRKRESRVFAGLIPGRALKGRRCFLLGTVILFLCLVSPVFA
                     GPADRVSESVLPNGLKVILLENHKVPLVTFQVWYRVGSRNENWGKTGLSHLLEHMMFK
                     GTKKVSGERFVRIIEENGGNNNAFTSHDFTAYYENLSADRVGVAIDLEADRMRHLVLR
                     EKDFRTERMVVMEERRLRTEDNPQAYLFEQVYAVAFAVSPYHWPVIGWMEDLQRLTLD
                     DLKAHYRTYYSPANAFLVVVGDFKKEEVLRMIRKAFGSYPKGARPPQERNAEPPQSGE
                     RRVCVEKEAKLPFVVKGYHVPNLSDPDSYVLEVIEALLSRGKSSRLYQNLVRERRLVL
                     EVDADHPLLSHDPGLFYLSAVPMPGKDVAEVEKAVDGELERLQREQVGDDELEKAKNQ
                     LEAAFVYGQDSLFSQAMMLARFEMAGGWRRLDDYLPAIRKVTAADVRRVAGKYFTRQN
                     STVGILIPLPPTEGRPAPQGVPGPDQMIR"
     gene            complement(11777..>12773)
                     /locus_tag="P5573_00310"
     CDS             complement(11777..>12773)
                     /locus_tag="P5573_00310"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HRT26539.1"
                     /translation="RDSKTDIALIKISSPFRDLPTLPLGDSDSVQVGEWVIAVGNPFG
                     LGNTVTQGIISATGRVIGAGPYDNFLQTDAPINPGNSGGPLVNLKGEAVGICTAIVAG
                     GQGIGFAIPINMAKTAVPQLKEKGKVVRGWIGVSIQTITPEIAQSLGLKSEKGALVAD
                     VVPDGPAAAAGIVTGDVIVSFDGREVKNFSDLSRYVAETAVGKTVSMRIIRSGKETEV
                     KVTVAEMTEERMAAQREGGVRKDIGIAVENITPQLQRQLGLKDRRGVVVVDVESGSPA
                     DMAGIQVGDVIREINRTKIGSLKDYEKVMAGVEKGKPVLFLITREGQTFFVSITF"
BASE COUNT         2652 a         3951 c         3363 g         2807 t
ORIGIN      
        1 gtccgaggac cataccggtg aacaacgcga ccagtacgat gatcgtcgtg gaccgcgccc
       61 ctatgtagta gatctgctct atgactttcg tccactgttt gggccgaaag atcatcaaga
      121 ctgccaggac gaggaagatc gtcgccgcgc cgagggtgtt gacccagttt atggtcttcc
      181 tgccgatagc tgcgaaaggc cgggtcagca agctgtctcg ctgcatactc tggtcttctg
      241 attggccgtc tttttctgtc acgctcatca cgaatcccgt tatcaacgca ggaatcaaac
      301 cgtagcgtac gacccatacc ataccttcag cgcaaaatca aaaactctct ctgctgtttc
      361 gcatgggtac ggcatgagag ttcagaaggc gtccttgttc gacttacaag ggcccccgtt
      421 tggcggcatg tgcttcgagg gcataatcga tgaccatatc gatgaatcgc gtttgattga
      481 tccccgatgc cgcggcctgg tggaagagaa ctgtggaagg ggtcatgccg ggaagggtgt
      541 tcggctcgag caccgtcacc cgcccgtctt tacggacaaa catgtcgacc cgggcatagc
      601 ccttgagccc gagggtcttg tacgcccgaa cagccacatc ttgaatactt ttcagaacat
      661 gttcgctaac tctcgcaggc gtcttgttct ctccctgccc atagagaaat ttgtcttcca
      721 gagaaaggat gtcctgagtg gggatggcct ccgaaggggt cagcgcgaag gggtcatcgt
      781 tgccgaggac gccgcaggtc acttccatcc cctcgatgaa ttcctccaca agcgccgtgt
      841 tgtcccactg aaaggcgccg atgagcgcct ccgggacccc ttccttcgat acgactttct
      901 tcacggcggt gctgcatccc tcacgggtcg gcttgattac gcaggggaac ccgacctcct
      961 tcgccaggcg gtcgagaagc gcctctttat cgcccgaatc ccaggcccgc cggtcaacgg
     1021 gcagcgtccc gggaacgtcg atcccactca tggcgaagag acgtcggcaa acgtatttat
     1081 ccatgcccag tgcggaaccg aggacaccag accccgtgta agggatcccc aggagctcta
     1141 agagaccttg gaggcaaccg tcttcaccgt atttcccatg gaggccgatg taaacaaggt
     1201 cgacccggga cttcagtcct tcataggcaa tcggggtcgc ttcctctttg aggtccgtct
     1261 ccacatcccg ggtgctgtta cgcatgacga gcttcaccgg tatttcccag aaggcccccc
     1321 gactgtccat gaagatgggg acggggtcgt actttttccg gtccatcttg gcaaagatat
     1381 tccttccgct ttcgagggat acttcctttt cagaagacat cccccccatg attactccca
     1441 cccggagttt cttttctcct ctgtcctcca tgaaaatttt ctccttatgc ccccaaagat
     1501 aatcgaccgg gtcgcaccct ttcataaggg ccccttctta tcccggtgga cccgtagggc
     1561 gatttcgatc aatctggtga tgatcatcga gggtagcatc cccgcctccg cggcctgctc
     1621 gaaaaagaac gatgaagggg ccatacccga agacgaattg gggtccgtca cgaggatgcg
     1681 cccgcccctc aagaaaaaac cgtcgatccg gccgtaacac cggaaaccaa gggccttgta
     1741 tgcccgcaag acctcggcct ttattctctc cgaatcctcg cgggagatat tcttcggcgg
     1801 ggtgaacttg cggcaacgcc cgggcatgta cttgtcgtca taggtataga attcgctctg
     1861 ggggtgtatc tccgtcaacc cgagcgccac cggctctccg tcgtccccct ccaagacgat
     1921 agacgaaaat tctatgccct ccaagaattc ttcaacaagg atagaattgt cccaacggag
     1981 ggcctccgtg acgccgcgct ccaagtcctc ctggcttcgg ttgatcttga cgccgacgct
     2041 cgacccctcg cggacgggtt tcgtcacaag ggggaaccca aaggcctcga caacctccct
     2101 tttcaattcc tcccgcctca catcccaatc tctttcatgg atgacgatgc tccggggaac
     2161 ggcaagcccc tcggcttcaa ggaccttctg ctgcaggtgt ttgtccatgc cgagggccga
     2221 ggccaggacg cccggcccgg tgtaaggaat gccgagaagc tccatcagcc cctggatgca
     2281 accgtcgtcc ccgtatttgc cgtgaagaga aatgaaggca agatctattc tttctttaag
     2341 ctcctcgtaa gcaacacgcc gggcctcttc cgcgagtcgc tcgctgacgt ccgtagtcgt
     2401 attctgggat attagctgcc agggcaactc ccagagcagg cccagcgaat ccataaaaat
     2461 cggtacgggc tcgtacagct cccggtccat gttgtcgtaa acattccttc cgctgttgag
     2521 cgacacctcc ttttccgaag acatgccgcc aaagatgata ccaacacgca gcttttccat
     2581 tcgcccttaa ccttctgcct tccccctgcc ctacaagtcc cagataaaaa tcacgccgct
     2641 cgtggggaag atccagtctc tgtccccgac cctgatgctg accgacttgc cttccgtttt
     2701 cacaacctcc gggtcggagc gccttcccga gaaaaagctg acctccctta cgtttctgat
     2761 ccgcgtgccg gcgtaattga aggaaatctc gagaccccgc gtcggataga cgagaaagac
     2821 cgagaagaca ttgctgctct tcagcttttt tgtcgtgatc tcaatcttga ccctaacggg
     2881 actgccgagt ttccggttca agaaatcgcc cccgcaccag acttcgaaac ccctgtccgt
     2941 cttctccgtg cggatgatgg gtatgccctc gccgtcaacg ctcacccgct cgaccttgaa
     3001 atcgctttcc ttgaacgtcg cgctctgctc attcaggagc catcggtact cgcagcgcgg
     3061 gtcggcaaag agggccgcga ggtgacggtt gttcatcgcg caaccgatca gaaagatgtc
     3121 gtttctcagt gtcttggtgt actcgacgtt ggtcgcgact ttcaagtagt cctccacact
     3181 gccgtcgacc gccttctctt cctcggaaaa ctccttcacg tgtacatcgt acctgaaatg
     3241 cgtccgcagc tcgagctgcc ggtcctcttt cccgaagagg gtctcgatca tctcgtagta
     3301 gatggcgtct cccagttcct cgctcctcgt ctttgcctga aaacaacggc ggatcatgcc
     3361 gtcgatgcgg gcgatggatt tttcttcatc ggggatgaat atcctcctca ggcgcggctc
     3421 gacggaacct gcctttccct gattaacgaa gacgcggggc gccctctttc ccagggcaca
     3481 gatgacctca tagctgatgg tctttgcctt ggcggctatc tcgttcgccg agatgtaccc
     3541 ttctccctgt cgtcccagga gaacgacctc gtccccgacc tcacattcag ggatatggct
     3601 gacgtcgagg gtgcacatat ccatcgaaac gcgcccgacc accggcgccc gctttccccg
     3661 aaccagggcc tcgccctgat tggagaggat aacaccgtag ccatcgccgt aaccaacggg
     3721 aatcgtggcg atcctggtag ggcctccggt cacgtatgtc ctgccgtacc caataccgta
     3781 ccccccggga aaatccttga gaaggacgac gctcgtcttg aagctcatga ccgggacgag
     3841 atgcgccttc gacacagtgc tctccccggg gtagatgccg taagtcatga tcccgggccg
     3901 caccatgtcg aggttgaacc gggggaaatt gaggacgccg ccgctattgg acatatggcg
     3961 gatcggtatc cggatgcctt tatcgtccag accactgagc aatcggctga aatgctccca
     4021 ctgcatctga ttgtaggtgt catcctcgac ctcgctcgac gacaggtggg tgaagatgcc
     4081 ttcgacactg atgtggggga accccagtat ctcctggatc atgggcaggg cttcgctgta
     4141 gatggtccct ccccgtccca tcccggtgtc cacctcgatg tgtacaggca gcctcttgcc
     4201 cgccttctcg aggccccgct ccagttcgcg ggcgaaatgg atatcggaga ccgagggcgt
     4261 caggttgtac tttatgatct ccttgacctc ggaatacgtc gccgggctca agatgacgat
     4321 gggggcgttg atgtccccca cccggagctg gacgccctcg tcggcgttcg caacgccgag
     4381 gaaactcgca ccgttcttca tggcctcatt agatatctcg atggccccgt gcccgtaagc
     4441 atcagccttc acaacctgaa gtatccggac gttcggccca accaggcgcc tgacctccgc
     4501 ccagttgtgc cggtaattgt cgaggtcgac ctcaacccaa ctcctgtatc tctgttctcc
     4561 ctgaccggcc atcatctatt gtcacttttt cgtcatcgtg aaaacaccgt cccagacacc
     4621 ctccggcgga ttggccttga ggtccgcgca tctctggatg tagagctccg aagggccgtc
     4681 gccgggtcga acctccagca cttttctgaa cccttcgatc gcctcatccc agagtccagc
     4741 cctgtacttc tccagggcgg actcgaaact cctgaccagg ggcgcccatc tttccgcgtc
     4801 cgccccgtcg ccgagaagct cgtagatctt gaccggcagt ttcttaccct tcacccggac
     4861 cgaatcgagc tcccggcaga cgagcttttc tttgacttca cggtgagtaa attcgctgat
     4921 cacgatgctg gtgccgtatt ccttgtttat cccctcgagg cgcgacccca agttcacatt
     4981 gtcccccatg accgtataat cgaaccgcat ctgcgacccc atgttgccta cgaccatgtc
     5041 accgctgttg atgcccacac cgatgtgcaa cgccggcttg ccctcttccg tccatttttt
     5101 gtgcaacacc cccagggcct ccagcatctc gagggcggtc ctgcaggccc tctccgcgtg
     5161 gtccggctgc tcgatcggcg ccccgtaaac ggccatgatg gcgtccccca tgtatttatc
     5221 gagcaacccg tcgtacttaa agacaatatt ggtcatggcg gtgagatact cgttgagcaa
     5281 ttgcacgagt tcttccggcg gcaggagttc cgatatcgtg gtgaaccccc ggatgtcgga
     5341 gaagagcacg gtcaggttct ttttgtcgcc gccgagcttc aatttcgagg ggtctttcag
     5401 caactcgttg atgacggaag gcgtgaggta gtactggaaa gcgccacgga ttttcttctt
     5461 ctccctctcc tcggttacgt atttatagac cgtgatggca agataaacga tcaagatcgt
     5521 acccacaggg tacacgaggt taagccaaaa attgaggcgg gagaagatgt aaccgttcaa
     5581 ggtgatgtaa gtcgcaagca ggaaaagggt caggaataca cccgccacgg ccctgagtct
     5641 cgggacgcca accccgatga cgagacctaa cgtcagaata gccgctatat cgaacaaacc
     5701 cgcccagccg ggccgttgga tgaagtcctg atgcaggatg ttgtcgatga cgttggcgtg
     5761 gacctcgacc cccggaaagg ccacgctgaa gggtgtcacc cgcaggtcgt atatccctac
     5821 ggccgttgca cccacgagca cgatcttgtc tttgaagagc tccggcgcaa ggcggccctt
     5881 caggatgtcg gcgatagagt aatggggaaa ggtcttcgcc cgcccgagat aattgaccaa
     5941 gatacgcccc gattcgtccg tggaaatggg tatgtccccc acctgaacac ccgccacccc
     6001 gaattcggcc acgtaaagag ccgtcatagg ataatcgagg tactggttga ccagggcaag
     6061 atcaagggag ggatagaaat cgtccctgaa tttgatcgcc agaggcgccc accggataga
     6121 cccatccgaa tcgggcagga cgttaaagta acccgcgctg tcggccgctt cggatatcac
     6181 gcgcacgttg ggctccgcgg cgtaagcatg gatcaaaaca gcttcgtctg ggttaccttg
     6241 aacctgtacc atctgatatt tgaaaggcgc cacaagctcc catccggtct cgatttccct
     6301 ttccgtcaga tgcgccgcct ccttcttggc catgtgaaag aagtaaccga gggtgacgtt
     6361 ccccgccttg ctgatcgact gcgcgagcgc cgcgtccgtg tcggcacgcg ccgctttctc
     6421 ccgcagtatt cgggtcagct tcgggttccc tattcccgcc ttctcaaact cacgggccaa
     6481 ttcctggata tccgcgagac tcgaatactg gtcgggttcg gcaaaaacga tatcgaaccc
     6541 cacagccttc gccccgtaag acttgaggac gtccacgagg tcggcgatcg tcgtgcgcgg
     6601 ccagggccac cggcccaatt ccttgacgct cttctcgtcc accacggcga tcaccgtctc
     6661 gccgcccgga gctcgctcgc cccgggagag gatcctgagg tcgagggtct tcagttccat
     6721 gaagcggaag aaaggagggt ccaggacgta cagaacgacg gctgctacga ttaggacgag
     6781 gctgatcttg aggggtgaaa gggagacgac cttttttaat cgatccttca tgaacgtcct
     6841 ttcctgttta aggccgggca aatactatca aacctgactc gaatgtcaag gcgatgcgcg
     6901 ggtatttacg gcccgggagg gacattccga aggggaacaa cggcgatata aaacttaagg
     6961 atcgttcttg acaattttaa ggtcttaata taggttcttt ctccaccgga caatcgtgat
     7021 gccgatgtag ctcagccggt agagcaagcg attcgtaatc gctaggtcag gagttcgaat
     7081 ctcctcatcg gctccagaat tcaaaaaaag ggcgcaccaa acaggcgcct tttttatttt
     7141 gggaacggcc gataaggcca tgcaccgcga tttagacgtt cacctcggcc acactcagac
     7201 gtgaagttcg acaacatccc cgtctctcag tacgtgattc ttctcgacct tctggccctc
     7261 gaacctcgcc gaaccccaga tgcgggcgta tttaagtttc gtcgcaaagt ccttgtggat
     7321 ccgttccgcc agttcgatca cggtgctccc ggaacggatg gtgaacggca gggagtagtc
     7381 cggctccttt ccgggcggtt tggagtagac ccggatgatc cccgagacat caaagatcgc
     7441 cctcttgagc tcttccaggt tttctttcct gtggacggaa acggtgatga ccgggagcgt
     7501 cgctccccat ttccgcttca gcgcttcgac gccttcatcg gcaccgggga ggtctttctt
     7561 gttggcgacg ataagcgccc ttttgtaaag cccaggccgg ccatcgagac cccggcccgt
     7621 cacgtggata ttccaacgct caatttgttc taggagaagc gccgcctggg tatccggttc
     7681 atcccccagg tccaagacca ggagaagtgc gtctgttgtc ctgaggatcc ccgacaccca
     7741 accgtccgtc gaatcattcc cgatgggcgg caggtccacg agttggatat ggatgtcttc
     7801 aaagggcatc atcccgggca ggggtagtac cgtcgaaaga ggataatcgg cgatgacggg
     7861 ctgggcgttg gtcaggcagg cgagcagaga cgatttcccc gtattaggga aacccaccag
     7921 ggccagttgg gccgcgcctt ccctctgaat gttgtaaagg ctgtcgcgac cccccttctt
     7981 cgactttttt agtgccaggg cctctttgtt caactgggag gccctgcgcc tcaggtcggc
     8041 ccgcagcttg tccgttccct tgtgcttcgg gacactggcg atgaggtctt caagggccgc
     8101 gagcttctcc gccagtgtgg cggcctgctt gtatttcttt tcggcctcga aatattccgg
     8161 tggaagattt gcgggcatgt tcaacccccg tttccggttt ccgttctcat caatagattt
     8221 cctgacagat gagtaaactc gacccttgag aaccggctct catgactttg caaacatcat
     8281 cttcaacgaa acaagaaaca gaataactcc gaagatacgc cccaatgtta catccgacag
     8341 gctcacggca atcttcgcac ctaaccatcc ccccgtaaaa agccccgcgc aaatcaaccc
     8401 ggccgctttc aggtcaacat atccctgatg ataataagtc agcgccgcca gtatcccgac
     8461 cggcggcacg agaagcgcga gcgtcgtgcc ctgggcaaga tgttgcgaca tgccgaaaaa
     8521 aaacaccaag gcgggaacga tgatgatccc accgccgagg cctatcaggc cgctcattat
     8581 cccggcaaaa agccccagaa ggacaagaag aaacgcattc accatcgtcg acaccccctc
     8641 cgcaacgaat cacacactcg gtaatgtcta tgtataccgg atgtccggcg atccttcaag
     8701 gattacagaa acagaattac ggtcggcccc agatgacgac cggacggtca gggcaaaccg
     8761 ggcgcgtcga gccccgcctc tttcaggtcc gccacgatca cgacgatgca tttatcgggg
     8821 tagagatacc gtttcgcaac gtcctgaatc ttttcgcggg caaccgccag aatgagagag
     8881 ggatatttag aggggtagtc aaggccaagg ccgaaatatt ccacctgtgc gagaaaaccg
     8941 gcgagcttag cctgggtatc catccgcatc gggaagcttc cggtcaggta tttcttcgca
     9001 ccgtcgagct ctttgacctt tacaggagag gcctgtatca gtttcatctg gtccaggacg
     9061 atccggatgg cctcacgagc cgagctgttc ttggtctgga tcgagacctg gaatgacccc
     9121 ggataccgac gggcgtcgaa gaagctaccg acggaatagg caagaccccg cttgacgcgg
     9181 atttcgtcga agagcctaga agaaaaacca ccgcccccta agatgtaatt catcagtacg
     9241 acggcataat aatccgggtt atccctgctc acccccgcat ggccgatcac gatattcgcc
     9301 tgggtgatgg agcggtttat cttaaccgtc ttcggtcctt ccgcgaaggc gctcttgaaa
     9361 ggaacggcca cgccatctcc ggccttccag gcatcgagaa ggggtaccaa acttgcgcgc
     9421 acttccgcga cggtcacatc accagccaca acgaggatgg cgttgttcgg gcaatagttt
     9481 tcccggtaga agcgctctac gtcccccctc gcaacggcgg gaagggattc cttcgtccct
     9541 tccacgggat ggccgtaacc gctgagatag agttctttct gaaaagcctt ctccgcaacc
     9601 atgtccggct ggtcttcttc agactggatc gcggccaacg ttttttccac ttctctcccg
     9661 acctcatcgg ccggaaagac aggacgggtc aggacatcca tgaagaggtc gaagcccctg
     9721 tccaggtcct tcttgagcac gcggaggctc aggaccgcat agtcgggccc cgccgcggcg
     9781 ccgagagaag cccccagaaa atcgagctcc cggttgatgg cctctgccgt cctcttctcc
     9841 gtaccgagga ggagtccctg ggcagtcaaa cgggcaaggc cctccttgcc gggcggatcc
     9901 ttgcgggacc ccgaatcgac aagaagctga agggtcgcga aggggagcga atgctcctcc
     9961 gctacgataa ccttcagctt gttcggcagg acgaaccttt ccacgggcgg gaaggcataa
    10021 gccggcgccg cgaccgtgat agaagctgct ataaaggtca ccgtcaggcc tgtaccgggt
    10081 ttaagattca aggttcccga ttcgaagcca tgaaaaatgt tccgcacctt ccaaaaaggc
    10141 cgcaggagca gttgcagtaa aaagcgagca gtcattttcc ctatcctgac tttgacccta
    10201 aaaaccgatt aaacactgtg tccgaggtcc gtgcctccat accgtatcat caccgtatca
    10261 tctgatccgg ccccggtacc ccctgcggcg ccggccttcc ctcggtcggc ggcaggggga
    10321 tgaggatgcc gactgtcgag ttctggcgtg tgaagtattt tccggcgacc ctgcggacat
    10381 ccgctgccgt tactttcctg atcgcgggca gataatcgtc cagccttctc cagccccccg
    10441 ccatctcaaa ccgcgccaac atcatcgcct gactgaaaag ggaatcctgg ccgtacacga
    10501 aagcggcttc gagctgattc tttgccttct caagctcatc gtcccccacc tgttccctct
    10561 gcaaacgctc cagctccccg tcaaccgcct tttccacctc ggcaacgtcc ttgccaggca
    10621 taggcacggc ggagaggtag aagagtccag gatcatggga taagagagga tggtcggcat
    10681 ctacctcgag gacgagccgc ctctcccgga ccaggttctg gtaaagccgg gagctctttc
    10741 ccctcgaaag taaggcctcg atcacctcca agacgtaact gtcggggtcg gagaggttcg
    10801 gtacgtggta acccttgacc acgaagggga gctttgcctc cttctcgacg caaaccctcc
    10861 tctcccccga ctgcggaggc tcggcattcc tttcctgcgg tggtctcgca cccttcggat
    10921 aggagccgaa ggccttcctg atcatcctga gtacctcctc cttcttgaaa tctccgacca
    10981 ctacgaggaa ggcgttagct gggctgtagt aagtccggta atgggccttc aggtcgtcga
    11041 gggtcaggcg ttgcaaatcc tccatccagc cgatgacggg ccagtggtaa ggactgacgg
    11101 caaaggcgac ggcgtacacc tgctcgaaaa gataagcctg ggggttatct tccgtcctga
    11161 gcctccgctc ctccatcacc accatcctct cggtccgaaa atccttctcc cggaggacca
    11221 agtggcgcat ccggtcggcc tcgagatcga tcgccacccc gacacggtcg gcgctcaagt
    11281 tctcgtaata ggccgtaaag tcgtgggatg tgaaggcatt gttgttgccg ccgttttctt
    11341 cgatgatgcg gacaaacctc tctccgctta ccttcttggt ccctttgaac atcatgtgct
    11401 cgagcagatg agagaggcct gtcttgcccc agttttcatt cctggaaccg acccggtacc
    11461 agacctggaa agtgacgagc ggcaccttgt ggttctcgag gaggattacc ttgagaccgt
    11521 tcggaaggac agactcggat acccggtcgg ccggcccggc aaagacaggt gagacaaggc
    11581 acaaaaacag gatgactgtt cccagtaaaa agcagcgcct gccctttagg gctcgacccg
    11641 gaatcaaccc agcaaaaact ctggattccc gctttcgtgg taataacatg cccattgcca
    11701 cgccctgtct tcccatctga accatagtga aggcctgccg cataggcgac gactctcgac
    11761 cttctttgaa ccctttttaa aatgtgatcg agacgaagaa ggtctgccct tccctcgtga
    11821 tcaggaacag cacgggtttc cccttttcga cccccgccat gactttctca tagtctttca
    11881 ggcttccgat ctttgtacgg tttatttccc tgatcacgtc gccgacctgg atgcccgcca
    11941 tgtcggcagg gctgccgctc tcgacatcga caaccactac accccttcga tccttgaggc
    12001 cgagctgccg ctgcaactgc ggagttatgt tctccacggc gataccgatg tccttcctga
    12061 cacctccttc cctttgagcc gccatcctct cctcggtcat ctcggctacg gtcaccttga
    12121 cctccgtttc cttgccggag cggatgatcc tcatggacac cgttttgcct acagccgtct
    12181 cggccacgta ccgtgaaagg tctgagaagt tcttgacttc cctgccgtcg aaggaaacga
    12241 tgacgtctcc cgtgactatt cccgccgctg ctgccggccc atcaggaacg acgtccgcca
    12301 cgagcgctcc cttttcgctc ttcaggccta aggattgagc aatctcgggc gtgatggtct
    12361 ggatggacac gcctatccac cctctgacga ccttcccttt ttccttgagc tgaggtactg
    12421 ccgtcttggc catgttgatg gggatggcaa atccgatacc ctgaccgccg gcaactatcg
    12481 ccgtgcagat gccgaccgct tcacccttca ggttcaccaa cggcccaccg ctgttgccag
    12541 ggtttatcgg ggcgtccgtc tggaggaaat tgtcgtatgg ccccgcaccg attacccggc
    12601 cggttgcact gatgattccc tgggtaactg tattccccaa accgaagggg ttacccacgg
    12661 cgattaccca ttcaccgacc tgaacgctgt cggaatcacc gagcggcaga gtcggcaggt
    12721 ctcggaaagg cgaagagatc ttgatcaggg cgatgtccgt cttggagtcc ctc
//
LOCUS       DAOTMX010000005        12548 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_4817_length_12548_cov_4.593452, whole genome shotgun sequence.
ACCESSION   DAOTMX010000005 DAOTMX010000000
VERSION     DAOTMX010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 12548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12548
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4817_length_12548_cov_4.593452"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(628..1704)
                     /locus_tag="P5573_00315"
     CDS             complement(628..1704)
                     /locus_tag="P5573_00315"
                     /inference="COORDINATES: protein motif:HMM:NF033581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HRT26540.1"
                     /translation="MSFKKTSRLSFADLALFNSMNRNRAIQRMEQINNTVNWSRIERL
                     VIRNYPVGKSAEGNDAYHPLLLLKCLLLQQWFRIDSDPELETQINDRISFKKFLGIPM
                     DENAPDHSTFSRFRSRLSKEIMKAVNHELLSQFAARGLSINEGIAVDARLVQSASRPL
                     GEDKLAEERSKREALEGCLDKSGKPMKFSRDLDSDWTVRNNVPHFGLKEHAAVDTRYG
                     FVLATEMTPASLHDSPLLPLCVAGSCHTPNPIQKVFADKGYFGKSNREFLRLNNIQDG
                     IMRKATRGAKLTAYEKERNKAIAKVRYIVEQYFGISHLYHHAHRARFTKLIKNAIDAL
                     FRQLAFNLSRGTKVLASQRQWCPI"
     gene            complement(1875..2201)
                     /locus_tag="P5573_00320"
     CDS             complement(1875..2201)
                     /locus_tag="P5573_00320"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HRT26541.1"
                     /translation="MKDDKLYPRHILEAIEKIESYTFVGRDFFMKTSIIPDAVIRNLE
                     IIGEASKQLSDSFKQRNADIPWRNVSGLRDILIHHYMGVDLEKVWNVVEQNLPQLKQA
                     VQKSIP"
     gene            complement(2198..2470)
                     /locus_tag="P5573_00325"
     CDS             complement(2198..2470)
                     /locus_tag="P5573_00325"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRT26542.1"
                     /translation="MADKREEILKVAAGHGARALMVFGSFARGEERPDSDIDLLVEMM
                     CDRSLLDIIAIKHALEDITHRKVDIVTEKALSPYIRDKILQDAVAL"
     gene            complement(2910..4172)
                     /locus_tag="P5573_00330"
     CDS             complement(2910..4172)
                     /locus_tag="P5573_00330"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRT26543.1"
                     /translation="MSDNLQVKRVLSRYLPKSTSKKVVLLTGARQTGKTTLSQASYPD
                     LRYINLDAPENRDILRLVPSSSWGRDVGSAVIDEAQKAPIVFEKVKYAFDAGHVSFCV
                     LTGSSQILLLKKIRETLAGRISLYELWPLMVGEVYHGGDDSDVKPPLIDKLLTGRSID
                     KILSPVPGILLDEKEAGQRAAENHLINWGGMPALLHLPEEERWKWLGDYEYTYLERDL
                     SDLARLDDLEPFRKFQRLSALRSGKLLQYSELARDAGVSVDTARRYLEYLRISYQCIL
                     IQPYRRNVTSSVIKTPKLYWVDVGILRRLTGFRGDPTGDLYETYVIGEIFKWIKTVQW
                     AGKLSFYRTRSGLEVDLLLETQHGVVGIEIKSRESVTGTDLRPLRDVANGLGREWLGG
                     IVVYRGREIRKMAEPSIWAVPSCRLFIR"
     gene            4480..4794
                     /locus_tag="P5573_00335"
     CDS             4480..4794
                     /locus_tag="P5573_00335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HRT26544.1"
                     /translation="MEIRISEKRADTMKILTGEIKAKGQLTVPKKIRDMLKLEEGQAV
                     TFLTIGDSVIISPKRLDVEEARRQIRRIVRESGLTEEALLSELKKEREAFYREKYGKN
                     RS"
     gene            4775..5233
                     /locus_tag="P5573_00340"
     CDS             4775..5233
                     /locus_tag="P5573_00340"
                     /inference="COORDINATES: protein motif:HMM:NF024861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRT26545.1"
                     /translation="MAKTEVKAFLDSNVIISGVISDRAAPRVILELLCHDLPFIRAAT
                     GQYNIEEIERTIAKKLPEMGTVLHDFMSRLIIEIIPLPSRKSLAKYRGVIADEDLPVL
                     VSALQYGADYLVTGDKRHFRGLAGKKDIPLKVCSPSEFVEIIATVVRELG"
     gene            5630..5926
                     /locus_tag="P5573_00345"
     CDS             5630..5926
                     /locus_tag="P5573_00345"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HRT26546.1"
                     /translation="MKMSEDIRPVTYLKSRTADLLAQVNKTKRPVIITQNGEARGVLQ
                     DTESYESMKAAIAILKLLVQGEEDIRKGDVIPHDEVFRRIEDRLKSATKNRGDE"
     gene            5919..6251
                     /locus_tag="P5573_00350"
     CDS             5919..6251
                     /locus_tag="P5573_00350"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRT26547.1"
                     /translation="MNKYSILWTKTAEQDMSRIIDYIGRDSLDRALEIFHAIQTSASA
                     LNSLPERGRIVPELKVHGISTYRELVISPWRLIYRIEGKRVFILTIIDGRRNLEDILL
                     ERLLYSDK"
     gene            complement(6665..7012)
                     /locus_tag="P5573_00355"
     CDS             complement(6665..7012)
                     /locus_tag="P5573_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT26548.1"
                     /translation="MLSEKEIRERAEYCHLVCLQLNWLDGNYDARPEQYLDYLKKSSL
                     GLADDEYIRTCIEEGLSSGKEDAGINELILVYESLALAFCEVLEISQEQLKESIPRDR
                     MKTLASEMGIVLE"
     gene            complement(7013..8230)
                     /locus_tag="P5573_00360"
     CDS             complement(7013..8230)
                     /locus_tag="P5573_00360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HRT26549.1"
                     /translation="MPKIILKPCPKAYILETHRHRPPDSTLHFVEGMKELLGMKDYRE
                     VTHIDRTGIPVYTCYRIRPDNSKTFHTGKGTSKTQAQVSLTVESIERYSSEYCNEYRE
                     RLLLDSFKGLMKKFNVLDPRDLHLSPHSEYTPDSPIHWIWGYDIARDEEILVPACEVF
                     HPYHLDEISVLNTHTNGLAAGNTMEEAVFHAMTEVIERDAWSIAKYNFLDGDALYIED
                     IPENRFLLDIVDKFIQAEIHVVAKDITSEIGVPVIAAFSRDMLHEDMMVIDGFGAHLD
                     PKVAMARALMELATTRALFIMKHGQEGLRESSLAYLDGHLEMEDHRFYASREKGLREM
                     EVGYNQDIYRDVQAVLEKLRAKGLDRTIVVDLSRKDTGIPTVKVIIPGTETYCFDRSR
                     RGNRLLVHDRTGD"
     gene            8745..9257
                     /locus_tag="P5573_00365"
     CDS             8745..9257
                     /locus_tag="P5573_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HRT26550.1"
                     /translation="MVDVGNSTQAVVSGLVSGVTYYFAVTDYNSVGESGFSNEVSYAV
                     PADTSPRDSNGTHSGSNAGTGGGGGCFIATAAYGSSLAPEVLLLRKFRDDHLLTNAPG
                     RLFVKLYCSLSPPLADFIRKDEKLRTATRLLLAPVVYAVKYPAAGFLGLVLLGIYGFR
                     SMTGRSRKFQ"
     gene            complement(9385..10038)
                     /locus_tag="P5573_00370"
     CDS             complement(9385..10038)
                     /locus_tag="P5573_00370"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRT26551.1"
                     /translation="MGTPANKQMIYIIGPRKLQNDILADYLEKELSAKCVVTADARHG
                     PPVGENGQGCPALVLLDSPEKDLDKYFMGLESASKRSLSEHIVALFNVKPGSGIEDKA
                     ISWGIRGFFYEQDSPKQFVKGITAMVKGEMWYPRDVMAKYILSRKDTNNSKKDVIVLT
                     QRELEILTMVISGSKNEDIANHLCISAHTVKTHIYNIFKKINVPNRLQAALWAAKNL"
     gene            complement(10704..>12548)
                     /locus_tag="P5573_00375"
     CDS             complement(10704..>12548)
                     /locus_tag="P5573_00375"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRT26552.1"
                     /translation="ALDEIKSSENDPKLAEAYFLLANIMLKNKNTAQAKDSLYKLLAQ
                     DEKNKTARLMIAQILESEKKYQEAEKEYLTLVRQEPDADEPGMLLARFYARTNRVNEA
                     EKILKDLIAAHPKKQEPRLLLGNIYGETKQYGPMEETLQSTIKDLPDKYAAYEVLSRY
                     YLSKNEKVKALKLQEQFISKAGSGPDTRKARMFAATLLLQDRKPDEALKHVNDVLKEN
                     AKDAQAKVMKGNILAGRRDFTGAIGEYRAVLKEEPGNIPALLGLARAHLMNNERSLAE
                     EAYRKVLETNPKEREALLALGDITLSKGDSNEAGKYYARFTELEPRQPVGYFKSGVVR
                     RAQNRNKEAVTFFEKTLALNPDYVPALAEIADIYAGERNLDGAVNRVRQQIAKSPKNP
                     GYQILMGRLQMAKRDYAGARKSLETALTMDPDSLEAMLAMAQLEQSEGSLDNALARYE
                     KLMAKNPDNAGIALLTASLLERKGDPVKAKAIYEDVLKKNPNAAPAANNLAFYYAEYE
                     PTKDNIAKAEKLIAPLLEKHANVPHVVDTAAWVYYRKGEYEKARGLLLSVYESASRFP
                     AIQYHLGMTYLRLGDNEKARTHLQLALKGREQYPGRTEAEKELKAISK"
BASE COUNT         2845 a         3033 c         3315 g         3355 t
ORIGIN      
        1 ctgcgcctgc tccaattttg agttttgaag tatgagtttt gaattatggc acgcagggct
       61 atctggtcca tctggtcttg tatgtttact tcagtctttc aaaatccgtt tcagttatct
      121 ggtctgtctg gtctgtttgg tctatcaggg tcgcaggctg cgcctgctcc agttttgagt
      181 gatgaccggg gcgcccagtt ctccagaggg gagaaccatg aataaatcat ccctcaccgg
      241 gggcgccgtc gagggggacc attatgaatt ttgagttgaa atagtagaag aaggaaaaaa
      301 agagggcagg ctgcttttct gagacgattt attgaaatac aaaaaaatac aaagcccgcg
      361 ggaaacggcg ggctgttcta ttgacggtaa aaagcttttc cccgatactt cggcgtcgcg
      421 gtaaaaacga aacgatgagc gatgatatta tgagaccttt gaacaacggt tgatttttgt
      481 aggggatggt tttgcaccgg aaatttttgg acggacgatg ttgacagggg ctgcggaatg
      541 tttcttgcgt gccggctcac attacgatga gttcatgttt gcctccctgt agcggtcgat
      601 ttcaatgtct ttgatgtcat tttgcactca tatgggacac cactgccttt gcgatgccaa
      661 caccttcgtt cctctggaga ggttgaaggc caactgtctg aagagcgcat cgatggcatt
      721 tttgatgagc ttcgtgaacc tggcccgatg cgcgtgatga taaagatggc tgatcccgaa
      781 gtattgttcg acgatgtacc ggaccttcgc gatggccttg ttgcgttctt tctcataggc
      841 ggtcaacttt gcgccccgtg tggcttttct cataatgccg tcctggatgt tgttcaggcg
      901 caggaattcc cggttgcttt ttccaaagta gcccttgtcg gcaaagacct tctggatggg
      961 atttggggta tgacaactgc cggcgacaca gagggggagc aaggggctgt catggagtga
     1021 ggccggggtc atttccgtgg ccaggacaaa accgtaacgg gtatcgacgg cggcatgttc
     1081 cttgaggccg aaatggggga cgttgtttct cactgtccag tcggaatcga gatcgcggga
     1141 gaacttcatc ggttttcccg atttgtccag gcagccctcc agcgcttctc gcttgcttct
     1201 ttcttcggcc agcttgtcct cgccaagcgg gcgactggcc gattgcacca gtcgggcatc
     1261 cacggcaatg ccttcgttga tggataatcc ccgggcggcg aattgggaca ggagttcatg
     1321 gttgacggct ttcatgattt ccttggatag acggcttctg aaacgggaga aggtgctgtg
     1381 atcgggggcg ttttcatcca tgggaatccc caggaatttt ttgaaggaga tccggtcgtt
     1441 gatttgggtt tccagttcgg gatcggaatc gatgcggaac cattgctgca gaagcaggca
     1501 tttcagaagc aacaggggat gataggcatc gtttccttcg gcgcttttgc cgacaggata
     1561 gtttctgatc accagccgtt cgatgcggct ccagttgacg gtgttgttga tttgttccat
     1621 tctttggatg gcacggtttc gattcattga gttgaaaagg gcgaggtcgg caaaggagag
     1681 gcgggatgtt ttcttgaagc tcatatttcc tcccgggggg gatagatttg ttggaaatat
     1741 ggcggcaaat ttgttgaatg tcaagtgata tcaatatatt atctttgcac aacgcccctt
     1801 gccgacccca aaagcggcac tcacatggcg aacacgaaat gaccatctca acgagttgtg
     1861 caaaggtctc attattatgg gatgcttttt tgaacagcct gtttaagttg aggaagattt
     1921 tgttccacta cgttccagac cttttcaaga tccacgccca tgtaatgatg aatcaaaata
     1981 tctcgaagtc ccgaaacatt ccgccatgga atatcagcgt tacgctgttt gaaactgtca
     2041 ctgagctgct ttgatgcttc tccgatgatt tcaagattac gaatgacagc gtccggtata
     2101 atcgatgttt tcatgaaaaa atcccttccc acaaacgtgt aggattcaat cttctcaata
     2161 gcctccagta tgtgacgggg atataattta tcgtctttca taatgctact gcgtcttgca
     2221 gtattttatc cctgatatac ggcgaaagag ccttctcagt gacgatatca accttgcggt
     2281 gagtaatatc ttcaagagcg tgcttgatgg cgataatatc gaggagtgac cggtcacaca
     2341 tcatctcgac gaggagatcg atgtcactgt caggccgctc ctcacccctt gcaaaggagc
     2401 caaaaaccat caatgccctt gccccatggc cggccgccac cttgagtatt tcttcacgct
     2461 tatccgccaa acttcgactg ataggcatac tattccactc cctcatatta tttacaagcc
     2521 tgagctgcga ctcattatct caagctatat tcttatcgat actttcttaa aaaatacgat
     2581 ttgtcaataa taattggcta gatagcaatg gcctgaaggg ccgactcgtt tgtttcgttg
     2641 gtttcgttat tttcatccgt ctaagtctgt ctggcccgtc tggtctatca gggtcgcagg
     2701 ctgcgcctgc tccaattttg agttttgaag tatgagtttt gaattatggc acgcaaggct
     2761 atctggtcca tctggtcttt ctggtccatt tggtctgtct tgtctatctg gtctgtttgg
     2821 tctgtgtaag tctgtctggg tcgcaggcag tgcctgcttc cagttttgag ttctgtttta
     2881 ccggcggggt ggatgaatgt ggactgaaat cagcggatga aaaggcggca ggagggtacg
     2941 gcccagattg agggttcagc catttttcgg atctctcgtc cgcgataaac gacaatacct
     3001 ccaagccatt cacgacccaa tccattcgcg acatcgcgca aaggccgcaa atccgtgccc
     3061 gttaccgatt cacgggactt tatctcaata ccgacaacac cgtgttgtgt ttccaggagc
     3121 aggtcgacct ccagacccga tcgggttcta tagaaggaca gtttgcccgc ccactgaacc
     3181 gttttaatcc acttgaagat ttcgccgatg acataggttt cgtaaagatc ccctgtcgga
     3241 tcgcctcgaa atcctgtaag ccgccgcaga atcccgacat caacccagta taacttgggt
     3301 gtcttgatga cagaactcgt aacattacgt ctgtagggtt gaatcagaat gcactgatag
     3361 gagatcctga gatactcgag atatctgcgg gccgtatcga cgctgacgcc ggcgtcccgg
     3421 gccaactccg agtattgcag aagtttgcct gacctgagtg cggacagccg ctggaattta
     3481 cgaaagggtt cgagatcatc gagccgtgca agatcggata agtccctttc cagataggtg
     3541 tattcgtaat ctcccagcca tttccagcgt tcttcttcgg gcagatggag taaagccggc
     3601 atgccgcccc aattgataag gtggttctcc gcggcccgct gcccggcttc tttttcatcc
     3661 agaaggatgc cgggaactgg actgagaatt ttatcaatgg atcgtccggt caagagtttg
     3721 tcgatcagag gtggcttaac atcactgtca tccccgccgt gatacacttc gcccaccatc
     3781 aaaggccaaa gctcgtaaag ggagatgcgc ccggccagtg tttcacgaat ctttttcagg
     3841 agcagtatct gactcgaacc ggtcaatacg cagaaggaca catgcccggc atcaaaggca
     3901 tactttactt tttcaaagac gatcggagct ttctgcgctt cgtcgataac cgcgctgccc
     3961 acgtctctgc cccaggaaga cgaaggaacc aaccgcaaga tgtctctatt ttccggtgca
     4021 tcaaggttaa tataacgaag gtcagggtat gaagcctgag acagggtggt tttacctgtc
     4081 tggcgtgctc ctgtgagcaa aacgaccttt ttagacgtac tttttggaag gtaccggctt
     4141 agaacccttt ttacctgtaa attatcgctc atggtcgtaa attatacaga tataattcag
     4201 tttggcaata tttttctgca gtattagaca atggccccgt agtcagggta aggggcaacg
     4261 gggtatctgg tctttctgat ctgtctggtc cgtctgatcc atctggtcct tctcgtctat
     4321 ctgagtcgcg ggctaagcct gctccaattt tgagttttga agtatgagtt ttgaatgaag
     4381 agaaaacagg gcgactgcaa tggagcagtg gatcgggggt catgaacggt cagggttgta
     4441 gatagaaaag attttgtaaa aatatcggaa ttctgatatt tggaaataag aatatctgaa
     4501 aagagggcgg atacaatgaa gatcctaacc ggtgaaatta aagcgaaggg gcagctgact
     4561 gttccaaaga agattcgaga catgctgaag ctcgaagagg ggcaggctgt tacgtttcta
     4621 accattgggg actccgtcat tatctcgccg aagaggctcg acgtggaaga ggcgagaaga
     4681 caaatccgaa gaatcgtaag agagtcgggc ctgactgagg aggctcttct gtcggaactg
     4741 aaaaaagaaa gagaagcatt ctaccgggag aagtatggca aaaacagaag ttaaggcctt
     4801 tctggactcc aacgtcatca tttccggcgt gatttctgac agggcagccc ccagagtgat
     4861 actggaactg ttatgccatg atctgccgtt catacgcgcc gccaccggtc agtataatat
     4921 cgaggaaatc gagcgaacga tcgcaaagaa gctccctgaa atgggcactg ttcttcacga
     4981 tttcatgtcg cgtttgatca ttgagattat ccctttgcct tcacggaaaa gccttgcaaa
     5041 atatcgcggt gttattgcgg atgaagacct gccagtgctt gtttcagctc tgcagtatgg
     5101 ggcggactac ctggtaacag gagataaaag acatttcagg ggtcttgcag ggaaaaagga
     5161 cattccattg aaggtatgct ccccatctga gtttgtcgaa attatagcca cagtcgtaag
     5221 agaactgggt taaaacaggc acaggctgtg cctgttttaa ttttgagtga tgaccggggc
     5281 gcacagttct ccagagggga gaaccatgaa taaatcatcc ctcaccgggg gcgccgtcga
     5341 gggggaccat tatgaattat tttcgtctat ttcgttcttt tcgtttgttt cgttggtttc
     5401 gttgatgtcg tctattcagt ctgttcggac tatgacacga agggcctcaa aagatagtga
     5461 ggagtgaata gtggtgagtg gtgagggtca cgggcaataa taatataggt ctggctatgt
     5521 caattcaggt cgtgctgcac ggcggaacac tgcagggtaa agggtttgga aagtggtttg
     5581 acatgaaaaa ccttttatgg tactaaattc ggtcaggagg tgacggttca tgaaaatgag
     5641 tgaagacatc cgcccggtta cgtacctgaa atcccgtacg gccgacctgc tggcccaggt
     5701 gaacaaaacg aagcgtccgg tgatcatcac gcaaaatggc gaggccagag gcgttcttca
     5761 ggacacggaa agctatgaga gcatgaaagc cgcaattgcc atcctgaaac tcctggtgca
     5821 gggggaggaa gacatccgga aaggtgacgt gatccctcat gatgaggtat ttcgacggat
     5881 cgaagatcga ctgaaatccg ctacgaaaaa ccgcggcgat gaataaatac agtattctct
     5941 ggacaaaaac ggccgagcag gacatgagtc gcatcatcga ctacatcggg cgcgacagcc
     6001 tcgaccgcgc tcttgaaata tttcacgcaa ttcagacctc cgcatcagct cttaattcat
     6061 tgcccgagcg tggtcgcatt gtaccggaat tgaaagttca tggaatatcc acctatcggg
     6121 aactcgtcat ctctccgtgg cggttgatct acaggataga aggaaaaagg gtgttcatcc
     6181 tgacgataat cgacggtcgt cgaaatctgg aagatatcct gctcgagagg ctcctgtact
     6241 cggataaata gctcggaaaa aaaaggggac aggctactat atgagtcgca ggctgtatgc
     6301 atggtccagt tttgaattat tttcgtctat ttcgttcttt tcgtttgttt cgttggtttc
     6361 gttattttcg tccgtctaag tctgtctggt ccgtctggtc tatcagggtc gcaggctacg
     6421 cctgctccag ttttgagttt tgaagtatga gttttgaatt atggcacgca gggctatttg
     6481 gtccctcttg tctatctggt ccgtctggtc tatagggtca tctatacgat cgataaggct
     6541 gaaaagacaa tcttgataca aaagtcggtg caagaaaaga tattgataag tagaaaaaat
     6601 agttatgaat tttaggtttt gaattaatta atcttgctaa ccactcacca ccagcactct
     6661 ccactcattc taaaacgatt cccatttcgg aggcgagggt tttcattctg tcgcggggga
     6721 tgctttcctt cagctgctcc tgactgattt ccaggacctc gcagaaggcc agggcgaggg
     6781 attcgtagac gaggatgagt tcgttgatgc cggcgtcctc tttgccggat gacaggccct
     6841 cctcaatgca tgtccggatg tattcgtcgt cggccagacc gagggaggac tttttcaggt
     6901 agtcgaggta ttgctcgggc cgggcgtcgt aattcccgtc cagccagttc aactgaaggc
     6961 agacgaggtg gcagtattca gcccgttcgc ggatttcctt ctccgagagc attcagtcgc
     7021 ccgtcctgtc atggaccagg agcctgttgc cccttcggct cctgtcgaaa cagtaagttt
     7081 ccgtcccggg aatgatgacc ttgacggtgg gtatccccgt atccttccgg ctcaggtcga
     7141 caacaatcgt cctatcaagg ccctttgcgc gcaacttctc gaggactgcc tgtacatccc
     7201 ggtagatgtc ctgattgtaa cccacctcca tctcacgaag gcctttttct cgtgaagcgt
     7261 agaagcggtg gtcttccatc tcgaggtggc catcgagata cgcgagcgag gattcccgga
     7321 gcccttcctg tccgtgtttc atgataaaga gcgctctggt cgtggcgagt tccatgaggg
     7381 cgcgggccat ggccactttc gggtcgaggt gagcgccgaa gccgtcgatc accatcatgt
     7441 cctcgtggag catgtctcgc gaaaaggccg cgatcaccgg cacgccgatt tcagatgtga
     7501 tgtccttggc tacgacgtgg atctcggcct gaatgaattt atcgacaatg tcgagcaaaa
     7561 acctgttttc gggaatatct tcgatgtaga gcgcgtctcc gtcgaggaaa ttgtacttcg
     7621 cgatgctcca ggcgtcgcgc tcgatgacct ccgtcatggc gtgaaagacc gcttcctcca
     7681 ttgtgtttcc ggcggccagg ccgtttgtgt gagtgttgag gaccgaaatt tcatccaggt
     7741 gataggggtg aaaaacctcg caggcgggga ccagtatttc ctcatcccgg gcaatgtcgt
     7801 acccccatat ccagtggatg ggactatccg gcgtatactc gctgtgaggc gacaagtgaa
     7861 gatcgcgcgg atcgaggacg ttgaatttct tcataaggcc tttgaagctg tccagcagaa
     7921 gcctttcccg atattcgttg cagtattccg aggagtacct ctcgatggac tctaccgtca
     7981 gggagacctg ggcctgggtt ttcgacgtgc ctttccccgt gtggaaggtc ttcgaattgt
     8041 ccggccgtat ccgatagcag gtatagacgg ggatgccggt acggtcgatg tgagtcacct
     8101 cccgatagtc cttcatgccg aggagttctt tcatcccctc gacaaagtga agcgtggagt
     8161 caggcggccg gtggcggtgg gtttccagga tgtaggcctt ggggcagggt ttcagtataa
     8221 tcttgggcat cggttccctc gttgaattcg gggactcatt atcctcaaaa gcattgtgaa
     8281 agtcaataca tgcgggggtc gttcttgccg ctgaaaaccg gtgacaacat attccctctg
     8341 aattataacc gaaatgcaca gaaaaactca aaaaattgcc gcttgacaat aaattacaaa
     8401 tatgcaaaca aaacagccgg ataacgcccc ggcaaaagat gtttctgttt cggggattgt
     8461 cttttttacg tgttgttttg cagtgacggc tcacaagttc aatcaacgcc tcagccacaa
     8521 gtagagctca acctgttatg cttctttgta ctggtcaagt acaaaagggg aggggctgta
     8581 tagccttttc cgtcgtcatt gctctactca ttgtcctttc cgtatcgcct tttatacaca
     8641 gagcacaggc cgctcaggtg actcttgcct gggatcgcaa cccagacccg agtgtaatcg
     8701 gatatcgtct ctattacggg gcagggaact actcttatta ttccatggtc gacgtcggaa
     8761 actcgactca agccgtcgtc tcagggctcg taagcggtgt cacttattat ttcgccgtga
     8821 cggattataa ctcggtgggc gaaagcgggt tttccaatga ggtttcgtac gctgtcccgg
     8881 cggatacgtc accccgcgat tcaaacggta ctcatagcgg gagcaatgcc gggaccggcg
     8941 gcgggggcgg ttgttttatc gcgactgccg cctacgggag ctccctcgca cccgaggtcc
     9001 tcctcctcag aaaattccgc gacgatcacc tcctgacaaa cgcacccggc agactctttg
     9061 tgaaattgta ctgcagtctg tcgccgcccc ttgccgactt cattcgaaaa gacgaaaaac
     9121 tgagaacagc gacgagattg ctcctcgccc cggtcgttta cgccgtaaag tatcccgccg
     9181 caggattcct tggcctcgtg ctgctcggta tatatggctt tcgaagcatg accgggcgat
     9241 caagaaaatt ccagtagcac ccctcacggc cgggatatct tccttcgtgc cgttctgttt
     9301 gctttcaccc cgaaaaaacg agaagtcccg tggtggagga tccacgggac ttccagaagg
     9361 agttatgaaa ggaggataat ggtcctatag attttttgcc gcccacagag ccgcctggag
     9421 gcggttgggg acgttgatct tcttgaagat gttgtagatg tgcgtcttta ccgtgtgggc
     9481 gctgatgcac agatggttgg cgatgtcctc atttttggag ccgctgatga ccatggtcaa
     9541 tatttcaagc tcccgctgag tgaggacaat gacatccttc ttgctattgt tggtgtcctt
     9601 cctggagagg atgtacttgg ccatgacatc cctcgggtac cacatctccc ccttgaccat
     9661 ggccgtgatg cctttgacaa actgcttggg agagtcctgt tcgtagaaaa agcccctgat
     9721 tccccaggag atggccttgt cttcgatccc cgagccgggt tttacattaa aaagcgccac
     9781 gatatgttcg gacaggcttc ttttgcttgc cgattcgaga cccatgaaat atttgtcgag
     9841 gtctttctcc ggcgagtcca gcagaaccag tgcgggacaa ccctggccat tttcgccgac
     9901 aggcggaccg tggcgggcgt ctgcggtgac gacacacttt gcgctcaact ccttctccag
     9961 atagtcggcc agaatatcgt tctgcagttt gcgtggccca atgatgtaaa tcatttgctt
    10021 gtttgctggt gttcccatgg ccccctctca aaatcttttt tattcaatca agtttcggat
    10081 agtttcccta tgctagatta gatcggcatc taatactttt tccttaggca cattacgtgc
    10141 caatcaataa aaaatattgt taaacaaaat aagttatcat ttaaaatcaa atggttagaa
    10201 tctaattatt ttatttttcc gtctaatgat aaagtagtat ttcctcagaa ttggaaagga
    10261 aacaggagga gccctacaaa acgtgaaggt caaagatcat gggatgagaa gaccaggcaa
    10321 aaagccgaaa attgaaaata cactgtatta tcgttatgtt atacagtttg tttgttatgg
    10381 ttatgtccgg ctcatacaga agctcggacg gcacgaaatt cacaatttga agtgatcttt
    10441 aaaaatagtg taatatcagc atgatacaaa aactacatta atttgtggcc gtttcctgtc
    10501 aatttttaga ggcgatttgc agatatagag aggataaata tacgacagac ctgaaaagtt
    10561 cctccgggct tgttctccat gaggcaaatc gaaaggggtc gaaatcaggg ttcagcgacg
    10621 gatgccaagc cggacaaagg cacactaacg gacgcagcac atctaaatca ttttgataaa
    10681 tattttagcc ggtgccgaaa aagtcatttt gatatcgctt tcagctcttt ttccgcctcg
    10741 gttcgccccg ggtactgctc ccggccttta agggcgagtt gaaggtgcgt cctggccttc
    10801 tcattgtcgc ccagcctgag gtaggtcatg cccaggtggt actggatggc cggaaagcga
    10861 ctcgcgcttt catagacgct gagaagaaga ccccttgcct tttcatattc gcccttccgg
    10921 tagtagaccc aggcggcggt atccacaacg tggggcacgt ttgcgtgctt ttcgagaagc
    10981 ggagctatca gcttttccgc ctttgcgata ttatccttcg tgggttcgta ttccgcgtaa
    11041 tagaaggcga gattgttggc cgcgggggcg gcattgggat ttttcttcag gacatcctcg
    11101 taaatagcct ttgctttcac aggatccccc ttcctctcga gcagcgatgc ggtcaaaagg
    11161 gcaatacccg cattgtcagg gttttttgcc atgagttttt cgtaacgggc gagggcatta
    11221 tcgagcgatc cttccgactg ctcaagctgg gccatggcga gcatggcttc gagactgtcg
    11281 gggtccatgg tgagcgctgt ctccagggat ttcctggcac cggcatagtc ccttttggcc
    11341 atctggagcc tgcccatcag gatctggtac ccggggtttt tcgggctctt cgcgatctgc
    11401 tgtctgacgc ggtttacggc accgtcaagg ttcctttccc ccgcgtaaat gtcggcaatc
    11461 tcagccaggg ccgggacgta gtcggggttc agcgcaagcg tcttttcgaa aaaagtaacg
    11521 gcttccttgt tcctgttctg tgcgcggcgg acaacaccgc ttttgaagta acccaccggc
    11581 tgtctgggct ccagttcagt gaatcgtgcg tagtacttgc ctgcttcgtt ggaatccccc
    11641 ttcgacagag taatgtctcc gagcgccaac agggcttctc tttctttagg gttcgtttcg
    11701 aggactttcc tgtatgcctc ctcggcgagc gacctctcat tgttcatgag gtgcgcgcgg
    11761 gcgagaccca gcagggcagg gatattgccg ggctcctcct tgagaacggc ccgatactca
    11821 ccgatagctc ccgtgaagtc cctgcgtccc gcgagaatgt tccccttcat gaccttcgcc
    11881 tgggcatctt tcgcattttc cttcagaacg tcattcacgt gtttcagggc ttcatcgggt
    11941 ttacggtcct ggagcagaag tgtggcagcg aacatcctgg cctttcgggt gtccgggccg
    12001 gagcccgcct tcgagataaa ctgctcctgg agcttcaggg ctttgacttt ctcgtttttc
    12061 gagaggtaat acctggagag aacttcgtaa gccgcgtact tgtcaggcag gtctttaatg
    12121 gtcgactgga gggtttcctc catgggaccg tactgcttcg tctcgccgta gatgtttccg
    12181 agcaggaggc gaggctcctg cttcttcgga tgagcggcga tcaggtcttt gaggatcttc
    12241 tccgcctcgt tcaccctgtt cgtgcgtgcg tagaacctcg ctagcagcat ccccggctcg
    12301 tcggcatcgg gttcctggcg gacgagggtg agatattcct tttccgcctc ctgatacttc
    12361 ttttcgcttt cgaggatctg cgcgatcatc aaccgcgccg tcttgttttt ctcgtcctgg
    12421 gcaaggagtt tatacaggct gtctttggcc tgcgccgtgt tcttgttctt gagcatgatg
    12481 ttggcgagca ggaaataggc ttccgcgagc ttcggatcgt tctcggacga cttgatttcg
    12541 tcgagagc
//
LOCUS       DAOTMX010000006        12117 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_5015_length_12117_cov_3.858067, whole genome shotgun sequence.
ACCESSION   DAOTMX010000006 DAOTMX010000000
VERSION     DAOTMX010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 12117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12117
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5015_length_12117_cov_3.858067"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /locus_tag="P5573_00380"
     CDS             <1..950
                     /locus_tag="P5573_00380"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRT26553.1"
                     /translation="TLLESELFGYEPGAFTGATTRKVGLFEASNGGTIFLDEIGDLPA
                     LTQSKMLRVLQEREVRRLGGRESIKVDVRVITATNKDLEEEMEKGRFREDLFYRLKVV
                     TIQLPPLRERREDIPELAGYFLERYNREFGKRIRKIDESALRALQEYHWPGNIRQLES
                     VIEKAVLMCDKDVITTGDIAGELVSPKRRSVFDIDIPENGLNFEEIEKELLKRAMVKC
                     DGVAAKAARLLGMSYKTFWYRWEKFGLETDLQEGSRFLPEDFDIPEGGLVFEEFEKEL
                     LKKAMTKANYVVARASRLLGLSDKAFRLRWEKFGLDSSQ"
     gene            1219..1491
                     /locus_tag="P5573_00385"
     CDS             1219..1491
                     /locus_tag="P5573_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT26554.1"
                     /translation="MKKVLAIIVSILFVLSVTGLCFAQAGAPAPAKDTKAVEKKADDK
                     KADDKKADKKVDKKDKKEKKAKKVKKEKKDKEMKEEAAPPAAPEKK"
     gene            1816..2493
                     /locus_tag="P5573_00390"
     CDS             1816..2493
                     /locus_tag="P5573_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT26555.1"
                     /translation="MAHLQSVVRNRDIGERIAFWAEQFVGTPYDRDPQGEYVSRRVIV
                     SDERVDCMYHVFRSLELALADTPVDAVKVALDKRFHTQGVLERGVVVNYEDRFQYGED
                     MIDSGKWGKEVTASVGKTMTVKGTRGKGPVEILSTEELCRRTGRLRSGDFIFFIRNPK
                     KRTQHELVGHIGIVKVEHGDGGRGDKVYLIHANGTKKKGGVVRKVLLDDYLRKTSHIG
                     ARITRYP"
     gene            complement(2513..3442)
                     /locus_tag="P5573_00395"
     CDS             complement(2513..3442)
                     /locus_tag="P5573_00395"
                     /inference="COORDINATES: protein motif:HMM:NF026288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRT26556.1"
                     /translation="MRSTSFIFIAILGAAAAVTACGGKPVKVTPPHLEAGTEQIIKGS
                     AYYKKGCYKQAFEHFFRSHELYTASNQMEGVAMSLNNLGTVYRATGDLESALACFDKT
                     IEIYRDIGNEAGERQALCNKAAVLVDLGRLDQAEAILDEVAGKPGGSSFLPLLNNRGV
                     LLTKKKAYADAERVLTEALKAAGPHGSALATVNSALGNLMLETKRFKEAAAYFDAALS
                     ADREEGFYRGMADDLRGIGRACFEMGERAKAVKYWEQSAKIYAVLGLGAEAGRTMTDI
                     RAAVPGTGVDTRITELFVKRWLEGKLYEKPCED"
     gene            complement(3450..4385)
                     /locus_tag="P5573_00400"
     CDS             complement(3450..4385)
                     /locus_tag="P5573_00400"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HRT26557.1"
                     /translation="MELTFKRQEKIVGVFVLTIIILMLASVVIVGRGKDWFKTYVTYY
                     TFFKETYNLKQGAPVKMSKADIGKVEQITLYEDRVRVKLLILEEYASRIKADSIAMVE
                     SPTFIGSEYVSIKAGSPEAPHLKEYDEIPSQAKKSIDDILAEFKIEETAKKVVAAVQD
                     LSALASSLSDPKGPLYTALNNINSILAHVEGVTRDINEGKGNVGELLKTRSLLEKVYA
                     ELDRVDKILANIEDGSRKVGTEIDRVDRILANIEDGSRDVPQITTSVKRGVSEIRQGV
                     KRIDSVVQAVQQNPLIKPSLPPEPKGEATDAGLRK"
     gene            complement(4399..5109)
                     /locus_tag="P5573_00405"
     CDS             complement(4399..5109)
                     /locus_tag="P5573_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT26558.1"
                     /translation="MKLIELTDYSLRPLGNGQGIYRFDFSLSEGDVCYINAGDDVGQL
                     FLRALATFETPLQGVYRYRGEVLDFSDYRHLLPIKRRIGYISSDVALISNRTLLDNLL
                     LSKTYFENTLSKELPDDMAELCRHFQLVDKLRLRPAQVSQGDRRIAAVIRELGKKPEV
                     LLLERPRDFMGFRIFELFQEILTDIIHGGTPVVFQAADEEFVWEVANREIRIAGGKLE
                     SLPFMTGNFNNKTPVTKR"
     gene            complement(5122..5910)
                     /locus_tag="P5573_00410"
     CDS             complement(5122..5910)
                     /locus_tag="P5573_00410"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRT26559.1"
                     /translation="MTFSLDQFFGKLGHNTIRRMNHLLDLCAFACRIIGLLIRRPTTG
                     RRLLNQIIVEQMYFTAVQALYVVVPVALIIGTMIIIQFAQVSSQYELGKITVILVVRE
                     FGPLITALVVILRSATAVTIETGYMKVLNEIESIELVGIDPVHIICPPRLIGITSAIL
                     CLFVIFDLVAIFGGYAVVWVITSIPLGDFMDQIAKAMTEADLFVGIAKGIFFGVAITV
                     ATIYRGFSVQRSVTDIPVATSRAAIDCLFYCLVINIFISAVFYL"
     gene            6320..10087
                     /gene="hrpA"
                     /locus_tag="P5573_00415"
     CDS             6320..10087
                     /gene="hrpA"
                     /locus_tag="P5573_00415"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HRT26560.1"
                     /translation="MRDLHRRRRVCIPRITYPEVLPITAKREEIVAAVRRHPVIVVTG
                     ETGSGKTTQIPKICLEAGRGVRGIIGHTQPRRIAAVAAARRLAEELGEPLGRSVGYKI
                     RFDERTGPGVCVKVMTDGILLAEIQSDPLLRRYDTIIVDEAHERSLNIDFILGILRNL
                     LKKRRDLKVVITSATIDTEKFSRAFDDAPVIEVSGRLYPVEVRYAPVDSDLEEGGDWT
                     YVDAAVEATVELKNDDAGDVLIFMPTEADIREACDLLQAKGDGDDVVLPLFSRLTWEE
                     QRRVFQPARRRKVIVATNVAETSVTIPGIRYVIDTGLARISRYNPRTRTLNLPVAPVS
                     RSSADQRKGRCGRVQNGVCIRLFSEEDYEGRPLYTPPEILRSNLAEVILRMLALNIGD
                     PSTFPFIDPPDGKSIRDGFEILKELGAIRQKGGGQGFELTERGRLMARMPIDPRISRM
                     LLEAAKENCLEEVAVIASALSIQDPREWPAGRLEEARRAHGAFNHPASDFMTLLNLWD
                     RYHETWSSFKTQGKMRKFCREHFLSYRRMREWVDLHGQIREILGEIGLLKQGVGKKDS
                     DYRALYDGIHRSILSGYLSHIARKKEKNLYTAARGREVMLFPGSGLFNRGSDWVVAAE
                     MVETTRLFARTAAHIESEWVEALAGDLCRRTYSDPHWERKRGEVVALEQVTLYGLPVV
                     AGRPVSYGPIDPAEASSIFIRHALVEGDMERPLPFLRHNLDLITKVSALEDKFRRRDL
                     LADEEAIADFYERRLGGVYDVRTLAKMIKEKGSDDFLRMKEEDLMRYSPDDDDLGRYP
                     DAVRLGKLKFRLKYRFEPTAPDDGVTLQVPRRLVPLVPARSADWLVPGLLREKVTLLL
                     KGLPKAYRKKLLPLAATVETVLREMPQGEGPLLSTLGDFLHRRFGVDIPASAWNPEAL
                     PDHLKMRFAVVDNKGKELVAGRDIHVLKEDIGEEIETNAFEKARSEWEKTNITRWEHG
                     YLPEVVTLGKAGSEGVAFPALEAGKEGVNLRLFRSLREAQAAHREGVIALFTLQFAKE
                     LKTLRRHLSIPKEMRPATVYFGGEEELEKTLYRRIMYDFFDASVRSEDDFLSRAEKAK
                     AEILARGQQVVQEIRPVLQAYRTTRTALHEMERANRANGAALAFLALLKEELDRLVPG
                     DFVIRYESDRLRRLPAYLEAITVRAQSGLLHLEKDREKAAKVKVFSDALERLREGLTP
                     FTSEEKLRAVEDFRWMVEEFRLSVFAQRIKTAFPVSPKRLEERLREIERMA"
     gene            10474..10671
                     /locus_tag="P5573_00420"
     CDS             10474..10671
                     /locus_tag="P5573_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT26561.1"
                     /translation="MGLALDELQEGDEVFKEKGFTFMINKELLEEVKPIKVDFIETPT
                     GSGYYVTANLASIGCGSCSCG"
     gene            complement(10720..11205)
                     /locus_tag="P5573_00425"
     CDS             complement(10720..11205)
                     /locus_tag="P5573_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT26562.1"
                     /translation="MGRAGKMVVVLAVLAFLAGCGPSLRLTPIPKEGEMQYVETREIV
                     KSPDKGTVCFIFDTFAGNLAGWYVYDNGKIVGASYENAYQCILTSPGLHTFWGDCDKH
                     NYATFNVDAGKIHYVIAKEVRENNAVVVNVNGANLYQVSEEVANPIMAKQKQCVFKGR
                     K"
     gene            11400..11750
                     /locus_tag="P5573_00430"
     CDS             11400..11750
                     /locus_tag="P5573_00430"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HRT26563.1"
                     /translation="MVFEVDFDKFINYKFLKFVEEGHVMALVKVRQNYQITIPAEVRK
                     NMKVGDYLEVERKNGELVLKEVKMVHSDQAYFYSKEWQEAEAEADSEIASGETVGPFD
                     GIDDALNALKKAKT"
BASE COUNT         2795 a         3328 c         3413 g         2581 t
ORIGIN      
        1 aaaccctgct cgaaagcgaa ctcttcggct acgagcccgg tgccttcacc ggggcgacaa
       61 cccggaaggt cggcctcttc gaagcctcga acgggggaac aatttttctc gacgaaatcg
      121 gagacctgcc cgccctcacg caatcgaaga tgctaagggt gctccaggag cgcgaggtga
      181 ggagactcgg aggcagagaa tctatcaaag ttgatgttcg ggtcatcacg gcaacgaaca
      241 aggacttgga agaagagatg gaaaagggca ggttcaggga ggacctcttc tacaggttga
      301 aagtagtgac gatccagctt ccgccgttga gggaaaggag agaggacata ccggagctcg
      361 ccggttattt cttggaaagg tacaaccgtg aattcggcaa gcgtataagg aagatagacg
      421 aatcagccct gcgagcactc caggagtatc actggccggg gaatatcagg cagctcgagt
      481 ccgtcataga gaaagctgtt ttgatgtgcg acaaggacgt catcacgacg ggcgacatag
      541 ccggagagct cgtctcgccg aaacggcgca gcgttttcga catcgacatc ccggaaaacg
      601 gcttgaactt cgaggagata gaaaaggaat tgctcaaaag ggccatggtc aaatgcgacg
      661 gcgttgcggc gaaggcggcc aggctgctcg gtatgagcta caagactttt tggtaccgat
      721 gggaaaaatt tgggctggaa acggatttac aagaaggcag ccgtttcctt ccggaggatt
      781 tcgacattcc cgagggtggt ttggtcttcg aggagttcga aaaagaactg ctgaagaagg
      841 ctatgacgaa ggctaattat gtcgttgcga gggcgtcccg tttgcttggc ctaagcgaca
      901 aggccttcag gctgaggtgg gaaaaattcg gcttggattc ttcgcagtag ttttaggtta
      961 ttatgcgata actcgcacac aatcctcaaa atggaatcta ttcttcttaa aagggacata
     1021 aaattcccga aaaggaagtt aaatttccca aaaggtaaga gaggttcccg aaaaggtaga
     1081 tgcagttgat tgattatgag gttgcaaaag aacaacaagt tgatattatt catcattcgt
     1141 taaggcgcca ttttggcatc attaatgctt gcataaaggg caattcgtta ttgagtccat
     1201 cataaaggag gtgatatcgt gaagaaggta ttagcaatca tcgtttccat tctcttcgtt
     1261 ctttctgtga cgggtctctg cttcgctcag gctggcgctc ctgctcctgc aaaggacacc
     1321 aaggcagtcg agaagaaggc tgacgacaag aaggctgacg acaagaaggc tgacaagaag
     1381 gtagacaaga aggataagaa agagaagaag gcaaagaaag taaagaaaga gaagaaggac
     1441 aaggagatga aggaagaagc ggctcctcca gcagctccgg agaagaagta agcgactttt
     1501 ttctcttcaa gatgttgatc taaaaggggt gctgagaaat cggtacccct tttggtttaa
     1561 aaaacccttt ttgtttcaca tctcctttga taacctgaac tgagggaata acgtttgcct
     1621 gtcaaatctc gtctttatgg aacgtttccg tcgacgaaag agatgcagta atcattgatg
     1681 gactcgcgaa aagtcgacaa tcaccatttt cgtcatgctg gtgtgcgccg ctcgccggca
     1741 gtcgtcgtat cgattatcgt ttttagtttt attttcctca ttccatccct tattcatgcc
     1801 tttacggatg ccgaaatggc gcacctccag tccgtcgttc gcaatcgaga catcggagag
     1861 aggatcgctt tctgggctga gcaattcgtc gggactcctt atgaccgaga cccacaggga
     1921 gaatatgtgt caaggcgggt gattgtttcc gacgagcggg tggactgtat gtaccatgtc
     1981 ttccggtcgc tggagctcgc ccttgccgat acgcccgtag atgcagttaa ggtggcgttg
     2041 gacaaacgtt tccacacgca gggagtcctg gaaaggggcg tcgtggtgaa ttacgaagac
     2101 cggtttcagt acggcgaaga catgatcgac agcgggaaat ggggcaagga ggttaccgct
     2161 tccgtgggca aaacgatgac ggtcaaaggt acgcggggta aagggcctgt cgagatactt
     2221 tcgacggaag aactctgtcg caggaccggc cggcttagga gcggagattt catctttttt
     2281 atcagaaacc cgaaaaagag gacccaacat gagcttgtgg gtcacatcgg gatagtgaaa
     2341 gtggagcatg gagacggcgg acggggcgat aaggtatacc tcatccacgc aaatgggacg
     2401 aagaagaagg gcggagtcgt caggaaggtg ctgctcgatg attacttgcg gaagacgtcg
     2461 cacatcggcg cacgaatcac ccggtacccg tagcgccggc gattaaactc gcctaatctt
     2521 cgcaaggttt ctcgtagagt ttaccttcca gccatcgttt gacgaagagc tctgtgatgc
     2581 gggtgtcgac gcccgtgccc ggaaccgccg ccctgatgtc tgtcatggtt cttccggcct
     2641 ccgcgcccag cccgagaacg gcgtaaattt tggcgctctg ttcccagtac ttgaccgcct
     2701 ttgcccgttc tcccatttcg aaacaggccc ggccgatacc cctcaggtcg tcggccatcc
     2761 cccggtaaaa accctcttcc cggtcggcgc tgagcgccgc atcgaagtat gccgccgctt
     2821 ccttgaagcg tttcgtttcg agcatgaggt tgcccagggc ggaattgacc gtcgctagcg
     2881 ccgaaccatg cgggcccgcc gccttcaggg cctctgtcaa caccctttcg gcatctgcat
     2941 aggccttctt tttggtcagc agaacccccc tgttgttcaa caaggggagg aaagaagaac
     3001 cgccgggttt gccggcgacc tcgtcgagga tcgcttccgc ctggtccagc cggcccaggt
     3061 cgaccaaaac cgccgccttg ttgcagagcg cctgtcgctc ccccgcctcg tttccgatgt
     3121 ctcggtagat ctcgatcgtt ttgtcgaagc aggccaatgc gctttccagg tctcccgtag
     3181 ccctgtatac cgtgccgagg ttgttgaggc tcatggcgac accttccatc tggttggagg
     3241 cggtgtagag ttcatgagac ctgaagaaat gttcgaaggc ctgcttatag cagccctttt
     3301 tgtaataggc gcttcccttg attatctgtt cggtgcccgc ctcaagatgg ggtggggtca
     3361 cttttaccgg cttcccgccg cacgccgtca cggcggcggc tgcgcccaga atcgctatga
     3421 agataaagga ggtgcttctc atcggcggct cactttctca agcccgcgtc cgtcgcctcg
     3481 cccttgggtt ccgggggcag gctgggtttt atgaggggat tctgctgaac ggcctggacg
     3541 acgctgtcga tcctcttcac tccctggcgg atctcactga cgccgcgctt gactgacgta
     3601 gtaatctgcg gcacatctcg gctcccgtct tcgatgtttg caagtatcct gtcgaccctg
     3661 tcaatctcag tgccaacttt ccggctcccg tcctcgatgt ttgcaagtat cttgtcaacc
     3721 ctgtcgagct cagcgtaaac cttttcgagc aatgacctgg tcttcaggag ctcacccacg
     3781 ttacccttgc cttcgttgat gtcgcgagtc acgccttcaa catgtgcgag gatgctgttg
     3841 atgttgttca aagccgtgta gagaggacct ttgggatcgc ttagactcga ggccaaggcg
     3901 gagagatcct gtacggccgc aaccaccttc ttggcagttt cctctatctt gaattcagcg
     3961 agaatgtcgt cgatggactt cttggcctga gacggaatct catcgtattc ctttagatga
     4021 ggtgcttcgg gcgacccggc cttgatggaa acgtactcgg acccgatgaa cgtagggctt
     4081 tccaccatgg ctatggagtc agccttgatg cgggaggcgt attcctccaa gatcaagagt
     4141 tttacccgaa ccctgtcctc gtatagtgta atttgctcca ccttgccgat gtcggccttc
     4201 gacatcttga ccggggcacc ctgcttcagg ttgtaagtct ccttgaagaa ggtgtagtaa
     4261 gtaacgtaag tcttgaacca gtccttgccg cgccctacga taaccaccga ggcgagcatc
     4321 aggattatga tggtcaaaac aaaaacgccc acgatctttt cctgcctttt gaaggttaac
     4381 tccatacgtc ccctatcgtc atcgtttcgt gacgggtgtt ttattgttga aatttcccgt
     4441 cataaaaggg agagattcca attttccccc tgcgatcctt atctccctgt tggcgacctc
     4501 ccacacaaac tcttcatccg ccgcctggaa gaccaccggt gttccgccgt ggatgatgtc
     4561 ggtcagaatt tcctgaaaaa gctcgaaaat tcggaatccc ataaaatccc gggggcgctc
     4621 gagtaaaagg acctccggtt ttttccccag ctcgcggatt acggcggcaa tacgcctgtc
     4681 accctgggag acctgcgccg gtcgcagacg caatttatct accagttgaa agtgccgaca
     4741 gagttcagcc atatcatcgg gaagctcctt tgaaagcgta ttttcgaaat acgtcttaga
     4801 gagaagcagg ttgtccagga gagtcctgtt gctgatcaat gcgacgtcgg acgatatgta
     4861 cccgattctt cgcttaatgg gcaagagatg acgataatcg gaaaaatcca gtacctcgcc
     4921 tctgtaccgg taaacgccct gcagcggtgt ctcgaacgtt gcgagggccc tcagaaagag
     4981 ctgcccaacg tcgtcgccgg cgttgatata gcatacgtcg ccttcggaca aagaaaagtc
     5041 gaatcggtaa attccttgcc cgttcccaag aggacgtaat gaataatccg tcaattctat
     5101 gagtttcacc gcgcctcttc gttacaagta gaatacggcg gaaatgaaga tgttgatgac
     5161 cagacagtaa aacaggcaat caatagccgc ccgggacgtc gccacaggga tgtcggtgac
     5221 cgaacgttgg accgagaaac ctctgtagat agtcgcaaca gtgatggcaa ctccaaagaa
     5281 gattcctttt gctatgccga cgaacaggtc ggcctccgtc atcgccttcg cgatctggtc
     5341 catgaagtcg cccaggggga tgctggtgat cacccagacg acggcatagc cgccaaatat
     5401 tgccactagg tcgaagatga caaaaaggca cagaatcgct gatgttatgc cgatgagacg
     5461 cggcggacag attatatgaa ccgggtcgat tccgaccagt tcgatcgatt ctatctcatt
     5521 cagcaccttc atgtagcccg tttcgatggt cacggccgtg gccgaccgaa gtatgaccac
     5581 gagagcggta ataagtggac cgaattccct tactacaagg ataacagtga tctttccaag
     5641 ttcgtactga ctcgagacct gggcaaactg gatgatgatc atggtaccga tgatcagcgc
     5701 cacggggact accacgtaaa gggcctggac tgccgtgaag tacatctgtt cgacgattat
     5761 ctggttcaac aaccgtcggc ccgtggtcgg tcttcggata agaaggccga tgatccggca
     5821 ggcgaaggcg cagaggtcga gaaggtgatt catgcgccgg atggtattgt ggccgagttt
     5881 tccgaaaaac tggtctagtg aaaacgtcat acaaacttct ttccgccgag gggtaatggt
     5941 tgctcccgag cggtaatttc ggaagtacca aatccgactt cgacgtcatg ccggcaaaag
     6001 ccggcatcca ggaatatcag ctttttacga catcactgga ccccggcttt cgccgggtga
     6061 cgacttttac atcataagtg tgtttatgca aagcaatcgc tgagaaccgt agacaattcc
     6121 accaactaaa tcaagccaaa tctgagaaac cgcatgaatc ttgccgggtt caagccggct
     6181 tgtgcgacgg cgctccgacg acgaatcccg ggtcatctac cgctcatcgg ctcccggttg
     6241 cggtcggacc cggcgttcgc cttgacaccg acaatcgtat cccataaaat tcctctaaat
     6301 ctatggttag aggctatttt tgagagacct acaccgacgc cgcagggtct gcataccccg
     6361 aatcacctat cccgaagtcc tgccgatcac cgcaaaaaga gaagagatag tcgcggccgt
     6421 ccgtcggcat cccgtgatcg tcgtcaccgg tgagacgggg tcgggaaaga ccacccagat
     6481 cccgaagata tgccttgagg cgggacgggg cgtcaggggg atcatcggcc acacccagcc
     6541 gagaagaatc gccgccgtcg ccgcggcacg ccggcttgcc gaggaactgg gcgagcccct
     6601 gggccggtcg gtggggtaca agatcaggtt cgacgaacgg acggggcccg gcgtttgcgt
     6661 aaaggtcatg accgacggca tcctcctcgc ggaaatccag tccgacccgc tcctgaggag
     6721 atacgacacg atcatcgtgg acgaggccca tgaacgcagc ctcaacatcg acttcatcct
     6781 cggcatactg agaaacctgc tgaagaagcg ccgggacctc aaggtggtga tcacctccgc
     6841 gacgatcgac acagaaaagt tctcccgtgc cttcgacgac gcccccgtca tcgaggtctc
     6901 ggggcgtctt tatcccgtcg aagtccgcta cgcgcccgtc gactccgacc tcgaagaggg
     6961 cggggactgg acttatgtag acgcggcggt tgaggccacc gtagagctga agaacgacga
     7021 cgcgggcgac gtcctcatct tcatgcccac ggaggcggac atccgcgagg cctgcgacct
     7081 cctgcaagct aaaggcgacg gagacgacgt cgtcctgccc cttttctcac ggcttacctg
     7141 ggaagagcag cggcgcgtct tccagcccgc ccggcggcgg aaggtcattg tggccacgaa
     7201 tgtcgccgag acgtcggtca ccatacccgg catccgctac gtcatcgaca ccggtcttgc
     7261 gcgtatctcc cgttacaacc cgcgcacccg cactctcaac ctccccgtcg cgccggtctc
     7321 ccggagcagt gcggaccagc gtaaaggccg gtgcggccgc gtgcagaacg gtgtctgcat
     7381 ccgcctgttc tccgaagaag actacgaagg gcgtcccctc tacacacccc ctgagatcct
     7441 gcgctccaac ctggccgagg ttatactgag gatgcttgcc ctcaacatcg gcgacccctc
     7501 cacctttccc ttcatcgacc cccccgacgg caagagcata cgcgacggat tcgagatcct
     7561 gaaggaactg ggggccatca gacaaaaggg aggcgggcag gggttcgagc tgacggagcg
     7621 ggggcgtctg atggcgcgga tgcccatcga cccccgtata tcgaggatgc tcctggaggc
     7681 cgcgaaggaa aactgcctgg aggaagtcgc cgtcatcgcc tcggccctga gcatccagga
     7741 cccacgggaa tggcccgcag gcaggctgga agaggcacgc cgcgcccacg gtgcgttcaa
     7801 ccaccctgcg tcggatttca tgaccctgct caacctctgg gatcgctatc acgagacatg
     7861 gagctccttc aagacccagg gcaagatgcg caaattctgt cgcgagcatt ttctctccta
     7921 ccggcggatg cgggagtggg tcgacctgca cggacagatc agggaaatcc tcggagaaat
     7981 cggcctgttg aaacaaggag tggggaaaaa ggactccgat taccgcgccc tctacgacgg
     8041 gatccaccgc tctatcctga gtggatacct gtcccacatc gccaggaaga aggagaagaa
     8101 cctctacacg gcggcacggg ggcgcgaggt gatgctcttc cccggttccg ggctcttcaa
     8161 cagggggagc gactgggtcg tggcggcgga gatggtggaa acaacgaggc tcttcgcgcg
     8221 gacggccgcc catatcgaga gcgagtgggt cgaggctctt gccggtgacc tctgtcgcag
     8281 gacctattcg gacccgcact gggagcgcaa gcgcggcgag gtggtcgccc tcgagcaggt
     8341 gactctctac ggccttcccg tcgtcgccgg ccgtcccgtc tcgtatggcc ctatcgaccc
     8401 cgccgaggcg tcttccatat tcatccgcca tgccctcgtc gaaggggaca tggagaggcc
     8461 gctgccgttc ctgcgccaca atctggattt aatcacaaag gtatctgccc tggaggacaa
     8521 gttccgccgc cgtgacctcc tcgccgacga agaggcaata gcggacttct acgaacggcg
     8581 cctcgggggt gtttacgacg tccggacact cgcgaaaatg atcaaggaaa agggctccga
     8641 cgatttcctc cggatgaagg aagaagacct gatgcgctat agtcccgacg atgacgacct
     8701 cggccgctat cccgacgcgg tccgtctcgg gaagttgaag tttcggctga agtaccgctt
     8761 cgagcccacg gcccccgacg acggcgtgac cctgcaggtc ccccggcgcc tcgttcccct
     8821 cgtgccggcc cggtccgccg actggcttgt ccccggcctc ctgcgggaaa aggtcacgct
     8881 gctcctgaag ggcctgccga aggcataccg caagaagctc cttcccctcg cagccaccgt
     8941 ggagacggtc ctccgggaga tgccgcaggg cgaaggaccg cttctatcca cgttggggga
     9001 cttcctgcac aggcgcttcg gggtcgatat ccccgcgtcg gcatggaacc ccgaggccct
     9061 gcccgaccac ctcaagatgc gtttcgccgt agtcgacaac aagggaaaag agctcgttgc
     9121 gggaagggac atccatgtcc tcaaagaaga catcggtgag gagatcgaga cgaacgcctt
     9181 tgagaaggcc cggtccgagt gggagaagac gaacatcacc cgctgggagc atggttacct
     9241 cccggaggtc gtcaccctcg gcaaggcggg gtcggaaggg gttgccttcc cggccctcga
     9301 ggcggggaaa gaaggcgtga acctccggct gttcaggtcg ctcagggagg cgcaggccgc
     9361 ccaccgggag ggggtcatag cccttttcac cctccagttc gccaaggaac tcaagaccct
     9421 caggagacac ctctctatcc cgaaggagat gaggcccgcg acggtctatt tcggcggtga
     9481 agaggaactc gagaagaccc tctacaggag gatcatgtac gacttctttg acgcctccgt
     9541 gagaagcgag gacgacttcc tcagccgggc ggaaaaggcc aaggccgaaa tccttgcacg
     9601 ggggcagcag gtcgtgcagg agataagacc tgtcctccag gcataccgga cgacacggac
     9661 ggccctgcac gaaatggaac gggcgaatcg ggcaaacggc gcggccctcg ccttcctggc
     9721 cctgttgaag gaagagctcg accgactcgt ccccggggac tttgtgatca ggtacgagag
     9781 cgaccggctc cgccgcctcc cggcttactt agaggccatc accgtgaggg cgcagagcgg
     9841 ccttcttcac ctcgagaagg acagggagaa ggcggcaaag gtaaaggttt tctcggacgc
     9901 cctggagaga ctccgggaag ggctcacacc cttcacgtcg gaggagaaac tccgggccgt
     9961 cgaggacttc cggtggatgg tggaggagtt ccgcctttcg gtcttcgccc agcggatcaa
    10021 gaccgccttc ccggtctccc caaagcgtct cgaagaacgc ctgagggaaa tcgaacgaat
    10081 ggcctgaata ttgtgggggc gattcgcgaa ccgcccctac gggaacccac cgtacaatga
    10141 accgcccgta ccattgacaa tcacaccgac agtgtttatc ctatggcgaa ataaaagaga
    10201 gaacctctga agaattttgc ccctttgtca tcccagcgaa agccgcactg tgccctcagg
    10261 agagggcggg gagccctgtc gtcggatgat cagcggacag tcaaaagtcc gtcatgacaa
    10321 atccgggtcg ttgttcaaag gtctcaaagg gagggcagca tgtttgaact gacggacaaa
    10381 gcgagtgaga tgctccggga gtatttcaag gacagggaag tcttaccttc catccgtctc
    10441 tttctcagcg agggcggctg atccgggccc gtcatggggc tggctctgga cgagctgcag
    10501 gaaggcgacg aggtattcaa ggaaaaaggg ttcaccttca tgatcaacaa ggaacttctt
    10561 gaggaagtca aaccgataaa ggtcgatttc atcgagacgc cgacggggtc tggttactac
    10621 gtcacggcca acctggcctc catcggctgc ggttcatgca gttgtgggta ggcccgcgtg
    10681 ttgtaggggc ggttcgcgaa ccgcccctac ggttttttct tattttctcc ccttaaaaac
    10741 gcattgtttc tgttttgcca tgatagggtt ggctacctct tcggaaacct ggtaaaggtt
    10801 cgcaccgttc acgttgacta ctacggcgtt attctcgcgc acctcttttg cgatgacgta
    10861 atggatcttc ccggcgtcta cgttgaacgt ggcataatta tgcttgtcgc agtcacccca
    10921 gaacgtatgc agcccagggg acgtgagtat acattgatag gcgttctcgt agcttgcgcc
    10981 cactatcttc ccgttgtcat agacatacca tccggctaaa ttccccgcaa aggtatcgaa
    11041 gataaagcaa accgtccctt tgtcggggct cttgacgatc tccctcgttt ctacgtattg
    11101 catttctccc tcttttggga tcggcgtgag cctcaaactc ggtccgcaac cggcaagaaa
    11161 cgccaaaacc gccaacacaa cgaccatctt tcccgctctg cccattcttt ctcccccttt
    11221 cctaaaacct tctttttgcc ctcataatga atatgcaaga atcccgcggc aactgttccg
    11281 tatgccagtc gcagcggtct tgtcaatgct tatttcacat ccggcgtctg cagggaaact
    11341 gttcgattta gccttcccat atgccgaagt aggtgcaccg aagactcatt gacgatggcg
    11401 tggtatttga ggttgacttt gataaattca taaattataa atttctcaaa tttgtagagg
    11461 aggggcatgt tatggcgttg gtgaaggtca ggcagaatta ccagataacg attcctgccg
    11521 aagtgcgaaa aaacatgaaa gtgggggatt atttggaagt cgagaggaaa aacggggaat
    11581 tggttttgaa agaggtcaaa atggtgcact ccgaccaggc ctacttctat tccaaggaat
    11641 ggcaagaggc agaggcagag gcggatagcg agattgcgtc gggtgagacg gtagggcctt
    11701 tcgacggtat tgatgatgcg ctcaatgcat tgaaaaaggc caaaacatga agcttgaatt
    11761 cacgaaggca tttatcaagg attaccaaaa gttgcccgca agtattcaga aaataacaga
    11821 taagaatctt gcattgctgc tttcaaactc gaaccacccg tcacttcacg taaaaaggat
    11881 gaatgacccg cgaaatattt gggaaggacg cgtgactgat tcgtacagat tcactttcca
    11941 ggtcaaaggc gacatatacc tcctcaggag aatcggaacg catgacatgc taagagagcc
    12001 atgaagacgg aacgaccact tgcagatcgc ctttacgtaa atcttgtggc ttcccctctg
    12061 ccggtaacag gaaagcccca tccgtttgag ggcggatgtt atctctttcg ctgtcag
//
LOCUS       DAOTMX010000007        12098 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_5024_length_12098_cov_3.646434, whole genome shotgun sequence.
ACCESSION   DAOTMX010000007 DAOTMX010000000
VERSION     DAOTMX010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 12098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12098
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5024_length_12098_cov_3.646434"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="P5573_00435"
     CDS             <1..262
                     /locus_tag="P5573_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT26564.1"
                     /translation="IAGRTPPRIRLPHNLVLVVAAIAEAWSRVSGKEPWVNLTSVRLA
                     RKKMYFSTEKAQLELGYRPRPIREAFRDAIDWYRQHGYIDGA"
     gene            252..1409
                     /gene="hpnI"
                     /locus_tag="P5573_00440"
     CDS             252..1409
                     /gene="hpnI"
                     /locus_tag="P5573_00440"
                     /inference="COORDINATES: protein motif:HMM:TIGR03472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohopanetetrol glucosamine biosynthesis
                     glycosyltransferase HpnI"
                     /protein_id="HRT26565.1"
                     /translation="MELDFLQWILLVPVIVGSVYGFLCLLTMRRFQAHASEMVEAPPG
                     GWPAVTILKPICGLDKGLEENLRSTCSQDYPEYQVVLSAQDENDPAIPVMQKIRQEFP
                     ERVDLVIENTVVGLNGKVNNLTGALRHARHDVLVISDSDTRLGRDYLKRIVAPLADRK
                     IGYVCTPYKATTAGRWYEKLCLLSFNADFVPGVIFAYVTGAAKFCLGSSVAVRREDLA
                     SIGGLEGLSEYLAEDYEMGRRLLDNGKRMVLVPYWVEILMDVKGAAQWWNYQVLWDQK
                     TRAADPVGFFSTVVTRSVPFAVLLAAYRLADWPGIAILGLAVLLRLAGAAAILKWEIK
                     DIEGLRALWLLPVRDVAALASWAVALTKRTVVWRNACLTLRRDGRLARRES"
     gene            1406..2257
                     /gene="hpnK"
                     /locus_tag="P5573_00445"
     CDS             1406..2257
                     /gene="hpnK"
                     /locus_tag="P5573_00445"
                     /inference="COORDINATES: protein motif:HMM:TIGR03473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid biosynthesis-associated protein HpnK"
                     /protein_id="HRT26566.1"
                     /translation="MRALIITADDFGFSPAVNEAVEEANVNGVLGTASLMVGAEEATD
                     AAERALRLPNLKVGLHIVLTGSRPVSPPSAIPDLVGRDGRLSSRLFASGVRFFFRPGA
                     RRQLEREIRAQFELFRETGLALDHANCHKHMHLHPTVAGLVLKVGAEYGLKALRLPYE
                     PARSWSVSCSAGKMASWLFLLPWAGLLRRKIRKAGVHSNDFVFGLGDTGSMGLQKVLR
                     FLDRLPPGVTEMYFHPAVRNCPELSRESGHSRHVLEFAALVDPAFRRALAAAKIRRIS
                     FCDLPLR"
     gene            2660..3772
                     /locus_tag="P5573_00450"
     CDS             2660..3772
                     /locus_tag="P5573_00450"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRT26567.1"
                     /translation="MEWTYLALISAALWFGILILPWRPWGTGEVLEANIPSSDEDLSD
                     ITVIIPARNEAGLIAATLSALDAQGKRLQIILVDDQSTDGTPQIARRTMGRNLRIVSG
                     KPLPAGWTGKLWAVEQGFSHVQTPLTLLLDADIELQPGILVALRKKMAEEGIQFISLM
                     AALRMKNFSERLLMPAFIYFFKLLYPFSLSNSSSSGVAAAAGGCILLETRLLDAIGGF
                     QALRGKLIDDCALAKRVKAHGFRTWVGITHSARSLRPYDHPRAIWNMVARSAFTQLRY
                     SFFLLLLCTVLMITAFWLPVAGLFFPSTTARAVSVASLGVMALSYLPTLTFYGVPKTF
                     AAAMPLIGTSYLAMTWTSAIRFWGKEGAEWKGRFYR"
     gene            3975..4103
                     /locus_tag="P5573_00455"
     CDS             3975..4103
                     /locus_tag="P5573_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT26568.1"
                     /translation="MTKDGRRVRYSICGMATKDDIKVGADKTLHLTAIPPALRNSR"
     gene            4147..5076
                     /locus_tag="P5573_00460"
     CDS             4147..5076
                     /locus_tag="P5573_00460"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HRT26569.1"
                     /translation="MAIPDYQSLMLPLLRFAAKKGTETSTSEAVEALAAELGLSDEDL
                     KEILPSGIQSTFVNRVGWASTYMKKAGLLEATRRGFYRITDRGRDLLKKQPEAINVKL
                     LKQYPEFLEFQRLKGTRGGDKATEPKGTSDLSAITPSEALESAYENLRDELAGELLGR
                     LKKISPSFFERVVVELLVKMGYGGSRADAGKAIGRSGDGGVDGIIKEDKLGLDVVYIQ
                     AKRWDNNPVGRPDVMQFAGALQAQRATKGIFITTSRFTDDARSYVSQIGSKIVLIDGE
                     QLTNLMIEYDVGVSTVSLYPVKKVDGDYFEENI"
     gene            complement(5128..5610)
                     /locus_tag="P5573_00465"
     CDS             complement(5128..5610)
                     /locus_tag="P5573_00465"
                     /inference="COORDINATES: protein motif:HMM:NF020543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1893 domain-containing protein"
                     /protein_id="HRT26570.1"
                     /translation="MTAPDFGSYSLAMFCGEERVFMSREPGLKPLAEWLDIFRGRHRN
                     CLLHDRVVGLAAARLITYSGLVTAVTARVASWPARRFLGGEGISLHAEQMVDSILTED
                     RTAVCPEEVVALETPDRDEYLRRIYRLIRYNPLEYELCRGQCGYTPWCRECMKSRGLL
                     "
     gene            6118..7218
                     /locus_tag="P5573_00470"
     CDS             6118..7218
                     /locus_tag="P5573_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRT26571.1"
                     /translation="MTDNNAAISSRLADRETLILDVQGPLNSACGKALARSFAENEGR
                     YQNVLWNFRLLDHMDSEGAILLLVNAAVLEQKKIGLAACHLSAPLRDVFRLTSLDAAM
                     EIFDTESEALERLPFRIGIRPRDPAFPAYKGPPVPGWAGSVERVAIGAVPAEAMNVNV
                     NGRKVTSPVNGFGRLWEKKYFIKTKNEAMTPQAVIALWRKEFSNFWPKGNRLFTSGGG
                     PIAPGATALLNLSLGGPIVMATGIVVIYADDDSFCFSTVQGHILHGWINFSSFRADDG
                     TIIQVHPIFRASDPIMEVGFRLGASRREDQFWHQTLGNLASRLGIEARVEQRTVLIDP
                     HVRWREAGNVWYCGAIRSSLYLLPYWLRRIFS"
     gene            7233..8651
                     /locus_tag="P5573_00475"
     CDS             7233..8651
                     /locus_tag="P5573_00475"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRT26572.1"
                     /translation="MGPHKQHYDAVVVGSGPNGLAAAITLRRKFPSVVLLEAKETIGG
                     GCRSAALTLPGFVHDVCSTVHPLAVSSAFFRSLELHRHGLSWIDPEIPLAHPFEDGSA
                     VFLHRSLDITADALARDGRAYKKLLQPFVERHERLLSSLLRPLPLSADPLLLAKFAVK
                     ALFPAHSLGQRTFATRRALALFSGLAAHAMIPLHQPATAAFGIILATLAHAVGWPFVK
                     GGSQNLSDALARCFLDGGGEIVTGRQVSSLEDMPAATYYFFDVAPKQLLDIHGLDLTE
                     HYRRRLAGFRYGPGVYKIDWALREPIPWKSDLCRRAGTIHLGNSHAEIAASIDCVNRG
                     EIPPEPYVVLAQSSLFDSSRSPNGWHTAWGYCHVPHGSDADMAASIENKIERYAPGFR
                     EVILAKNTISAKTMETYNMNYVGGDINAGKQDIFQLYARPVLSLNPYRTSKRNIFICS
                     SSTPPGGGVHGLCGYYAARTIS"
     gene            complement(8818..10254)
                     /gene="hpnJ"
                     /locus_tag="P5573_00480"
     CDS             complement(8818..10254)
                     /gene="hpnJ"
                     /locus_tag="P5573_00480"
                     /inference="COORDINATES: protein motif:HMM:TIGR03471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid biosynthesis associated radical SAM
                     protein HpnJ"
                     /protein_id="HRT26573.1"
                     /translation="MKTLLLNPPSFAGFDGGAGARYAATREITAFWYPTWLAYPAGLI
                     EKSRLLDAPSHGITPEETTEIGKDYDFAVIFSSTAGFANDVRLAEMMKAANPGLMTAF
                     VGPHVTVLPEESLQVGRSIDFVVRKEFDYAVAEFAGGKKLEDIPGLSFRKNGRVVHNP
                     DRPPLEDLDALPFAVDVYKRDLDFRRYNLPFLKYPYVSFYTSRGCPAQCTFCLWPQTM
                     TGHRLRTRSVDNVTAEVKRAMELFPEAKEIFFDDDTFTWSKKRVLDLCRAFRPLKFTW
                     SCNARVDADYEVLREMRAAGCRLLVVGFESGDPVILKNIKKGTTVEQAREFMKNCRRL
                     GFKVHGDFQVGHPGETRESIERTVRFAIDLDPDTIQVSVSHPFPGTRFYNYLVENGYL
                     TSADMADSQGHQLPNIEYPGLERGEIAEAVHDFYSRFYFRPRIIFRIVSGALWDSSDR
                     RRLVQEGKEFLKFRAQSRENIRRLKALP"
     gene            complement(10329..>12098)
                     /locus_tag="P5573_00485"
     CDS             complement(10329..>12098)
                     /locus_tag="P5573_00485"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRT26574.1"
                     /translation="MVTLIMALSWCFGLTTLLIGHLNILSITFAPLILGLGIDYGAHW
                     FARYQEEEESGLHSTKSELLRETMENIGPGILVAGLSLAVSFLPLTMTGFKGLAELGV
                     ICCTGMVVTNIASLTVLPALVMIFDSPRFGRRATGQSPINPMYRMKPKWTIVILAVGT
                     AALIISVWQTSNVRFDLNMLHLQSPDVESVVWEEKLLKGSELSSIFGQILEPSLEEVR
                     AKTKALKSLPTVSRVESVEDLLPHEQEAKLKMLKRVRPILDGIAFSPTAAQTVDVKAL
                     KEVLGRIRFKMLDTEGVGEKLKNQMVEVRTIIDEIREKVDSSEREEVQKALVLFEKSL
                     IHDLDDKLQMVRSNVNARPMTVNDLPHSLLERLVSSNGHFLIRVYPKGDVWDPQFLGR
                     FVQDLRSVAPNAVGDPVTLYVFTREFQISVIKAALLSGAFMFVFLLFVMREFKSAFLS
                     IAPLLVGSVWTFGLMPPLGINLNLANSIFLPLIVGAGVEYGIIIIYRWRQKEEGKAPS
                     TVLPSSTAWGVVLAGLTTTFGFGSLMISSHRGIFSLGLLTTVGSLAVLVASVVFLPAV
                     LEVLQLRKDVFMKGGPDLQENRD"
BASE COUNT         2594 a         3479 c         3441 g         2584 t
ORIGIN      
        1 gatcgccggc cgcacccctc cccggatacg cctgccccat aacctggtcc tggttgtcgc
       61 tgcgattgcg gaagcctggt ccagggtcag cggcaaagag ccctgggtca acctgacgag
      121 cgtccgcctc gcccgcaaga aaatgtattt ctcgacggaa aaggcgcagc tggagcttgg
      181 ttaccggccg aggccgatca gggaggcctt ccgcgatgcc atcgactggt accggcaaca
      241 cggttacatc gatggagctt gactttctcc agtggatact cctggttccc gtcattgtag
      301 ggtcagtcta cgggtttctc tgtttgctca cgatgcgacg ttttcaggcc catgcctcag
      361 agatggtcga ggcgccgccc ggcggctggc cagccgtgac tatcctgaag ccgatctgcg
      421 ggctcgacaa gggcctcgaa gagaacctgc ggagcacctg ctcgcaggat taccccgagt
      481 accaggtcgt cctgtccgcc caggatgaga atgaccccgc tatcccggtc atgcagaaga
      541 ttcggcagga gttcccggag agggtcgacc tggtcatcga aaataccgtg gtgggtttga
      601 acggcaaggt caacaacctc accggggcgc tgcgccatgc aaggcacgat gtattggtga
      661 taagtgacag cgacacccgg ctcggccgcg actacctgaa gaggatcgtt gcgcccctcg
      721 cagaccggaa aatcggttac gtctgcaccc catacaaggc aaccacggcc ggtcgatggt
      781 atgaaaaact gtgtctcctc tccttcaatg ccgacttcgt tcccggcgtc atcttcgctt
      841 acgtgaccgg ggccgcgaaa ttttgcctgg ggtcgtcggt cgcggtaagg cgggaagacc
      901 tggcgagtat cggaggactt gagggtctct ccgaatacct ggctgaggat tacgagatgg
      961 gccgacgtct cctggacaac ggaaagcgga tggtccttgt cccttactgg gtggaaatac
     1021 tgatggacgt caagggcgcg gcccaatggt ggaactacca ggtcttatgg gaccagaaga
     1081 cgagggcggc cgaccccgtc ggtttctttt ccaccgtcgt gacccgctcc gtgcccttcg
     1141 cagtccttct ggcggcttac cggttggcgg actggccggg gatagcgatc ttgggcctgg
     1201 ctgttctcct ccgcctggcc ggcgcggcgg cgatactgaa gtgggaaata aaggatatag
     1261 aaggactgag ggcgctgtgg cttcttccgg ttagggatgt ggcggcgctt gcttcctggg
     1321 ccgtcgccct gacgaaaagg accgtggtct ggcgtaatgc ctgccttacg ctcagaaggg
     1381 acggcaggct ggcgaggagg gagtcgtgag ggcgctaatc atcaccgccg acgacttcgg
     1441 cttttccccg gcagtgaacg aggcggtcga ggaagctaat gtaaatggcg tgttaggtac
     1501 agccagcctg atggtcgggg cggaagaagc gacggacgca gcggaaagag cgctacgcct
     1561 gccgaacctc aaggtaggac tgcacatcgt actcaccgga agccgacctg tttcgccccc
     1621 ttctgcgatc cctgatctgg tcgggcggga cggcaggctc tcctcgcggc tcttcgcatc
     1681 gggggttcgg tttttttttc gcccgggcgc ccgccggcaa ctggaacggg agatccgtgc
     1741 ccaattcgag ctctttagag aaaccggcct cgctctcgac catgccaact gccacaaaca
     1801 catgcatctc catcccacgg tcgccggcct cgtgctgaag gtcggcgccg aatacgggtt
     1861 gaaagccctc cggcttccct acgagcccgc aaggtcgtgg tcggtttcct gctcggccgg
     1921 gaagatggcc tcctggcttt ttctcctgcc ctgggcgggc ctgttgagaa gaaaaatccg
     1981 gaaagcgggt gtgcactcga acgacttcgt cttcgggctg ggcgataccg gctcgatggg
     2041 gctacaaaag gtcctgcgct ttttggatcg tctgccccca ggggtgacgg agatgtattt
     2101 ccaccccgcc gtccgcaact gcccggaact tagcagagag tcgggccaca gccggcatgt
     2161 cctcgaattt gccgccctgg tcgaccccgc cttccgccgg gcgcttgccg ccgcgaaaat
     2221 caggcggatc agtttttgcg acctgccgct cagatgaatc cggaggcacg gctcaagaaa
     2281 cccagaaaaa ctctttcact tcacccgccc gtcgtaagcg ctatctatac cgaaatcatt
     2341 caaaaatcat cccgcgaatc accttgactt tcctcatgcc tctggctaac atctgaaccg
     2401 taaaaatggg aggcataaga gtatacagaa gtattaatgc tggggctata aatatcgcag
     2461 actaaattcc aacaactggt tcaacccgat cggaaaaagc atcgacgggt taaccgaagc
     2521 gttatgtccg ccgaatcacc ccctgcctca tggacaccct gaaacagggc ggttttttta
     2581 tgagttcagc aaacttcagt ctgtcaagcg gaaagttgcg atcttggggt aaattcttcc
     2641 cccaaccatc cggaggagca tggaatggac atatctggct ttaattagcg cagcgctctg
     2701 gttcggcatc cttattttgc cctggcgtcc ctggggtaca ggagaggttc tagaggccaa
     2761 cattccctct tctgatgaag atctcagtga tataacggtc atcatccccg cccggaatga
     2821 agcaggactg atcgccgcca ctctgtctgc attggatgct caggggaaac gactccagat
     2881 catcctggtg gatgaccaat cgacagatgg gacgcctcaa atagcccgcc ggacgatggg
     2941 tcggaatctg cggatcgtct caggtaaacc tctaccggca ggctggacag gcaaactctg
     3001 ggctgtcgag caaggattca gccatgtcca gacgccgctg accttgctcc tcgacgccga
     3061 tatcgagctg caaccgggca ttcttgttgc gcttcgaaaa aagatggccg aagagggcat
     3121 ccagtttatc tctcttatgg ctgccctgcg catgaaaaac ttctcggaac gattactcat
     3181 gccggccttc atctatttct tcaagctcct ctaccccttc agtctttcca attccagttc
     3241 ctccggagtg gctgccgctg ccggcggctg catcctgttg gaaacccgcc tgctcgacgc
     3301 tatcggcggc ttccaggcct tgcggggcaa gctgatcgac gattgcgccc ttgccaaacg
     3361 ggtcaaggcc catggttttc gcacctgggt cggcatcacg cattctgcgc gaagtttacg
     3421 cccatacgac catccgaggg cgatttggaa catggtagcc cgctctgcct tcacacagct
     3481 tcgctactcc tttttcttgc tgcttttgtg cacagtcttg atgataacag cgttctggct
     3541 gcctgttgcg ggtttgtttt tcccgtcgac cacggccagg gccgtttccg ttgcctctct
     3601 tggggtcatg gccctgagtt atctgccgac gctcacgttc tatggagtgc ccaaaacctt
     3661 tgccgcggcc atgcctctga tcggtacttc ttaccttgcc atgacatgga catcggcaat
     3721 ccgtttctgg gggaaagaag gcgccgagtg gaaggggcgg ttctacagat aaacgcttca
     3781 cgccgggaat attaaggatt gttggcgtga attttccacg tcatgttacg cggcgtggtg
     3841 tccgggtact tgggcatttt ttcatgatga cgaggatcag cgccatttgc tcgagtcaac
     3901 tcaagacagc gatgccttgc cggagattga aaagagcaac acgaacggga agaccggcgg
     3961 gagaacatga tttcgtgacg aaagatggaa ggcgggtccg ctattcgatc tgtggcatgg
     4021 caaccaaaga cgatataaag gtcggagcgg acaagactct ccacttgacg gctattccgc
     4081 cggcgctccg taacagcagg tgatattggc agctggggac agataaaatc ataaggagaa
     4141 aaacgaatgg ccatcccgga ttatcagagc ttgatgttgc cgcttcttcg gtttgcggcg
     4201 aaaaaaggaa ctgaaacatc gacaagtgag gccgtcgaag ccttggccgc ggaattgggc
     4261 ctttccgacg aagacctcaa ggaaattctc ccaagcggga tacagtcaac ctttgttaac
     4321 cgcgtcgggt gggcatcgac gtacatgaaa aaggcagggc tccttgaagc gacacgcaga
     4381 ggattttatc gaatcaccga ccggggaaga gacctcctca aaaagcaacc cgaggccatc
     4441 aatgtaaaac tcctcaagca atatcccgag ttcctcgaat ttcaaaggct caaagggacg
     4501 agaggtggcg ataaggcaac cgagcccaaa gggacatctg acctttcagc aattactccg
     4561 tcagaggcct tggaatctgc ctatgaaaac ctccgggacg aattggccgg tgaattgctc
     4621 ggcaggctca agaaaatatc cccgtccttt ttcgaacgcg tcgtcgtgga actgcttgtc
     4681 aaaatgggtt acggcggttc gcgtgccgac gctggtaagg ccatcggccg gagtggcgat
     4741 ggcggagtgg atgggatcat aaaagaggat aaactcggcc tcgacgtcgt ctatattcag
     4801 gccaagcgat gggataacaa cccggtcggt cgccctgatg tcatgcagtt tgcgggtgcc
     4861 ctccaggccc aaagagcaac taagggcatt ttcatcacta cttccagatt cactgatgat
     4921 gcgcgaagct atgtttccca gatcggcagc aagatcgttc ttattgatgg cgaacaactt
     4981 acaaatctga tgatcgagta tgatgtcggc gtatccaccg tttcgttgta tccggtcaaa
     5041 aaggtcgacg gcgattattt cgaagaaaac atctaacacc gcgtcaaccc ggaccggcaa
     5101 tttcgccttg ctccatcccg cccctcatca caaaagcccc cgagatttca tacactcccg
     5161 gcaccagggc gtatagccgc actgcccccg gcagagctcg tattcgaggg ggttatacct
     5221 gatgagccgg tagattcgcc tcaagtactc gtcccggtcc ggcgtttcca gagcgacgac
     5281 ctcttccgga cagaccgctg ttcggtcttc ggtgaggatg ctgtcgacca tctgctcggc
     5341 gtgaagggat atcccctccc cgcccaggaa gcgccgggcc ggccaagagg ccacccgggc
     5401 cgtcacggcg gtcacaagcc ctgaataagt aatcagcctc gccgccgcca gtccgacgac
     5461 tctgtcgtgg aggaggcaat tccggtgcct gccccggaat atgtccagcc attccgccag
     5521 gggcttgagg cccggttcgc gggacatgaa aacccgctcc tccccgcaaa acatagccag
     5581 cgaatagctc ccgaaatcag gcgccgtcat cgacgcctcg ccggggcgga aattgacccg
     5641 cggggggaag agagacgcct ccggggccgg gttgccaatg aaggccgcgt cgcggtggtg
     5701 gggccgcgac cgcggccggg agcgaatctt ttttcatcca tcagtgcccc ttctttcgat
     5761 aggtgacgcc ttccgccgca ggcgtatcat cccggatctg tatttgcctg tagcgcaata
     5821 cgcctgaaaa ggtcaactgg aaacagacac ctgcggctgg tatgcccaca tgaatggcga
     5881 cggcgagaaa cattgccgga tactgacggt gtcggacgat gacgaccgtc acaggcgaag
     5941 tttttgcgtt cgacggcctc tctcggcatc acatcctacc acagttgtat gtagacgtat
     6001 tcagtgacat aaattagtat tgaacagacg tgattttatg ctaacctgcg gggagaataa
     6061 tcggtgtacg attcggcttc cgtaaagacg cgggaaagaa aggaaagggt gcagcctatg
     6121 actgacaata acgcggccat aagctcgagg cttgccgacc gggaaaccct gattctggat
     6181 gtgcagggac cgctgaatag cgcctgcgga aaagccctgg cgcggtcatt tgccgaaaac
     6241 gaaggaagat atcaaaatgt cctttggaat ttccgccttc tcgaccacat ggattcagaa
     6301 ggagcaatcc tcctgcttgt caatgccgcc gttcttgagc aaaaaaagat cgggctggca
     6361 gcctgccatc tctccgcgcc tctcagggac gtctttcgcc tgacatctct cgatgcggcc
     6421 atggagatat tcgacacgga atcggaggca ttggagagac ttccttttag aatcggaatc
     6481 cgcccccggg accccgcatt tcctgcatac aagggacctc ccgttcccgg ctgggctgga
     6541 agcgtcgaac gcgtcgctat cggcgccgtt ccggcggagg cgatgaacgt caatgtcaac
     6601 ggcagaaaag taaccagccc cgtcaacggc ttcggtcgcc tttgggaaaa gaagtatttc
     6661 atcaagacca aaaacgaagc tatgacgccg caggcggtca ttgccctctg gcgtaaagag
     6721 ttctcgaatt tctggccaaa aggcaacaga ttgtttacct ccggcggcgg tcccatcgcc
     6781 cccggcgcaa cggcgcttct gaatctaagt ctaggtgggc caatagtgat ggcaaccggc
     6841 atagtcgtga tctacgccga tgacgactcc ttttgcttct ccacggtcca ggggcacata
     6901 ctccacggct ggataaactt cagcagcttc cgtgcggacg atggaacgat catccaggtg
     6961 cacccgatct tccgcgcctc cgaccccatc atggaggttg gctttcggtt gggcgcgtcg
     7021 cgacgggagg atcaattctg gcatcagacg ctcggcaatc ttgcctcgcg ccttggcatc
     7081 gaggccaggg tcgaacagcg cactgtgctg atcgaccctc atgtccggtg gcgcgaggcg
     7141 ggaaatgtct ggtattgcgg tgcaatccgg tcttctttat atctgcttcc gtattggctg
     7201 cgacgaatct tctcttagga gctgccaaga aattgggtcc tcacaagcaa cattatgacg
     7261 ctgtcgtcgt gggcagcggc ccgaacggcc ttgctgccgc gataacgctc cgcagaaaat
     7321 tcccgtcggt tgttttgctg gaagcgaaag aaaccatcgg tggcggctgc cgatccgcgg
     7381 cgttgacgtt gcccggtttc gttcatgacg tctgttccac ggttcatccg ctggcagtct
     7441 catcggcttt ttttaggtcg ctggaacttc accgccacgg cctttcctgg atcgaccccg
     7501 aaataccgtt ggcccacccg tttgaggacg gttcggccgt ttttctgcac cgctccctgg
     7561 acatcactgc cgatgccttg gctcgggacg gccgagcata caaaaaactc ctgcagccct
     7621 ttgtcgaacg ccacgaaaga ctgttatcct cgctgttgcg gccactgccc ctatcggccg
     7681 acccgctttt gctggccaaa tttgcggtga aggccctttt tcccgcacat agtcttggac
     7741 aaaggacatt cgcaacccgg cgcgctctgg cgcttttttc cgggctcgcc gcccacgcca
     7801 tgatccctct tcaccagccc gcaacggcgg ctttcggaat catccttgcg acattggccc
     7861 atgcggtggg gtggcctttt gtaaaaggcg ggtcgcagaa cctgtcagat gccttggccc
     7921 gttgtttcct ggacggcggc ggagaaatcg tcacgggcag gcaggtcagc tctttggaag
     7981 atatgccggc ggcgacgtac tatttctttg acgtcgcccc taagcagtta ttggatattc
     8041 acggtttgga ccttactgaa cattaccggc ggcggttggc cggatttcgt tacgggccgg
     8101 gcgtctataa aatagactgg gccttgcggg agcctattcc ctggaagtcc gacctctgcc
     8161 gaagggcagg cacaatccac ctgggaaatt cgcacgcgga gatcgcggct tccatagatt
     8221 gcgtaaacag gggcgaaata ccgccggagc cgtacgtggt actggcgcaa tcgagtctct
     8281 tcgactcttc acggagcccg aacggctggc atactgcctg ggggtactgc catgttcccc
     8341 acggttcgga cgcggatatg gccgcttcga tagaaaataa aatcgaacga tacgcccctg
     8401 ggtttcggga ggtcatcctg gccaaaaaca ctatatcggc gaaaacgatg gaaacataca
     8461 acatgaacta tgtgggagga gacatcaacg ccggcaagca ggacattttt cagctttatg
     8521 cacggcccgt cttatccctc aatccctacc ggacatctaa gcgcaatata ttcatctgct
     8581 cgtcatcgac gcccccgggc ggcggggtcc acggcctttg cggctactat gcggcacgga
     8641 ccatatcctg acactgaata caggatgact tggcagggct taaaaaagac gagacggcat
     8701 tcggatcaat gaccagtggc gtgtatgttt tcgctggtag gaaggcgatg cttattaagg
     8761 aaggtattcc aagtccaggg cgcataatgg tcattattga acgattaaga aacgggatta
     8821 cggcaaggcc ttgagcctgc ggatgttctc cctactctgg gcgcggaact tgaggaactc
     8881 tttcccttcc tgcaccaacc tcctgcggtc gctgctgtcc cagagggcgc cgctcacgat
     8941 cctgaagatg atccggggac ggaagtaaaa acggctgtag aaatcatgga cggcttcggc
     9001 gatttcccca cgctccaggc cggggtactc tatgttcgga agctgatggc cctgtgaatc
     9061 agccatatcc gccgaggtca ggtacccgtt ttccaccaga tagttgtaga atctggtgcc
     9121 cggaaaggga tgggataccg atacctggat ggtgtcgggg tcgagatcaa tggcgaaccg
     9181 gacggtcctt tcgatactct cccgcgtctc tcccgggtgc cccacctgaa aatcaccgtg
     9241 taccttaaaa ccgaggcggc ggcagttttt catgaactcc cgcgcctgct cgacggtcgt
     9301 cccctttttt atgttcttca ggatgacggg gtctccggat tcaaacccaa cgacgagcag
     9361 acggcaacct gctgccctca tctcccgcaa gacctcgtaa tcggcatcga ccctggcatt
     9421 gcaggaccat gtgaatttca gcggcctgaa agcccggcat aggtcgagga cccttttttt
     9481 gctccaggtg aacgtatcgt cgtcgaagaa tatctccttg gcttccggga agagctccat
     9541 cgcccttttt acctcggccg tgacattgtc gacagacctg gtcctcaacc tgtgcccggt
     9601 catcgtctgc ggccacaggc agaacgtaca ctgcgccggg caaccgcggg aggtgtagaa
     9661 cgagacgtag ggatacttga ggaaaggcag gttgtatctc cggaagtcga ggtcgcgttt
     9721 gtaaacatcc actgcaaagg ggagcgcatc caggtcttcc agcggcggtc ggtccgggtt
     9781 gtggacgacc cgcccgtttt ttcggaagct taggccgggg atgtcctcga gttttttacc
     9841 cccggcaaat tcggcgaccg catagtcgaa ttccttcctc acgacaaaat cgatcgaccg
     9901 acccacctga aggctttcct ctggcaggac ggtcacgtgc ggtccgacaa acgccgtcat
     9961 caacccggga ttggcggcct tcatcatctc cgccagccgg acgtcgttgg cgaaaccggc
    10021 ggtgctcgag aagatcacgg caaaatcata atctttaccg atttcggtcg tttcttccgg
    10081 ggtgatcccg tgagagggcg cgtcgagcag gcgactcttc tctatcaggc ctgccggata
    10141 agcaagccag gtggggtacc agaatgcggt gatctcgcgc gtagccgcat aacgagcgcc
    10201 cgcgccgccg tcgaaccccg caaaagacgg ggggttaagc aagagggtct tcatgcacag
    10261 ttcccatgaa tgtttttact ttcattagct ttcctttttc cgaatgtcaa tgccctcggc
    10321 aaactttgtc agtcccgatt ctcctgaaga tcaggacctc ccttcataaa gacatctttc
    10381 cggagttgca gtacctcgag cacagctggg agaaagacca cggaagcgac gagcacggcc
    10441 aggctgccga ccgtagtaag caggccgaga ctgaaaatgc cccggtggga agagatcata
    10501 aggctgccga aaccgaaggt cgtggtcaag ccggccagca ccacgcccca ggcggtgctg
    10561 gacggcagca cggtgctcgg cgccttgcct tcttccttct gcctccaacg gtagatgatg
    10621 atgattccgt actcgacccc ggccccgacg atcagcggca ggaatatgct gttggccagg
    10681 ttcaggttga tcccgagcgg gggcatcaag ccgaaggtcc agacactccc caccaagagc
    10741 ggagcgatcg agagaaaggc ggatttgaat tcccgcatca cgaacaggag gaacacaaac
    10801 atgaaggcgc cggagaggag ggcggccttg atgacactga tttgaaattc ccgggtgaag
    10861 acataaagcg tgaccgggtc ccccaccgcg ttgggggcga ccgaccggag gtcttgtaca
    10921 aagcggccga ggaactgcgg gtcccaaaca tcgcccttgg gatagacccg gatgaggaaa
    10981 tggccgttgc ttgacaccaa gcgctcaagg agtgaatgag gtaggtcgtt cacggtcatg
    11041 ggccgggcat tgacgttgga cctgaccatc tggagcttgt cgtccaggtc atgaatcagg
    11101 ctcttttcga acaggaccaa ggccttctga acctcctccc gctccgacga atcgaccttc
    11161 tcgcggattt cgtcgatgat ggtcctgacc tcgaccatct ggttcttcag tttttccccg
    11221 accccctcgg tgtcgagcat cttgaacctg atccttccca gaacttcttt caaggccttt
    11281 acgtcgacag tctgggcggc ggtcggcgaa aacgcaattc catccaggat aggccggacc
    11341 cttttgagca ttttcagctt cgcctcctgc tcatggggga gcaaatcctc cacgctctcc
    11401 acccgtgaga ccgtgggcag agattttagg gccttcgtct tcgccctcac ctcctcaaga
    11461 gagggctcca gtatttgccc gaagatgctg gaaagctcgg agcccttgag cagcttctcc
    11521 tcccatacca cggattcgac gtcgggcgac tggaggtgga gcatattcaa gtcgaatcgg
    11581 acattggagg tctgccagac cgagattatc aaggcagcag taccgacggc caaaatcaca
    11641 atggtccatt tcggcttcat acggtacatg gggtttatcg gggattgacc ggtggcacgc
    11701 cttccgaacc ttgggctgtc gaatatcatc accagggcag gaaggaccgt cagagatgca
    11761 atgttggtaa caaccattcc cgtgcaacag atgacgccca gctcggcaag gcccttgaag
    11821 cccgtcatcg tgagcggcag gaacgaaacg gcaagactca gcccggctac aaggatgccc
    11881 gggccgatat tctccatggt ctcccggaga agttcgcttt tcgtcgagtg gaggcccgac
    11941 tcctcttcct cctgatagcg ggcgaaccag tgggcgccgt agtcgatgcc aaggcccagt
    12001 atcagcgggg caaatgtgat ggagagtata ttcaggtggc cgatcagcaa tgtcgtcaac
    12061 ccgaaacacc aggaaagggc catgatgagc gtcaccat
//
LOCUS       DAOTMX010000008        11922 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_5113_length_11922_cov_4.476532, whole genome shotgun sequence.
ACCESSION   DAOTMX010000008 DAOTMX010000000
VERSION     DAOTMX010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 11922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11922
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5113_length_11922_cov_4.476532"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="P5573_00490"
     CDS             complement(<1..533)
                     /locus_tag="P5573_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT26575.1"
                     /translation="MKSKRILVAVLCVALGIALMSCASNKEPAAAAIKTAEDALNAVK
                     GEAMKYVPDKVKAVEDAITAAKGSFDKKDYDTALNTAKAIPDKVKELATAAATKKAEL
                     TKGWEELSAGLPKMLDAVKSRLDILSKSKKLPANLDKAKLEGAKSGYEAAVKMWDDAK
                     THFSGGNLTDAMAKAATV"
     gene            complement(553..762)
                     /locus_tag="P5573_00495"
     CDS             complement(553..762)
                     /locus_tag="P5573_00495"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRT26576.1"
                     /translation="MAGEEKPKGGGADAWDATQWHVVQKGETLSKISKKYYGDPNLYM
                     KIFEANKDILKNPDLIKVGQKLRIP"
     gene            complement(824..1255)
                     /locus_tag="P5573_00500"
     CDS             complement(824..1255)
                     /locus_tag="P5573_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT26577.1"
                     /translation="MKQKFLYVITGAGVTLLFYSIILFLAINPRIQSSGEAPESYMGI
                     AFMVIFPVCFLIGSFVSGYLVQPLLEKRSAMDFLWVSPGFYAALVFLVPTLLQARTLN
                     RFFLFFFLLSAMIWMVASFAGTRLGVFFRDQKHKPGAVPGT"
     gene            complement(1346..1903)
                     /locus_tag="P5573_00505"
     CDS             complement(1346..1903)
                     /locus_tag="P5573_00505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HRT26578.1"
                     /translation="MRYFLCFIFILCIVSPALGAETFTLTSPDFKHRATLQNDQVYNS
                     FGCSGKNLSPALAWKNAPAGTKSFALTVYDPDAPTGSGWWHWVVYNIPATVTSLPKGA
                     GTPDGKLLPAGSLQTNTDFGGPGYGGPCPPVGHKPHRYIFTIHALKVEKIDLPPNATA
                     AMTGFFINQNAIGKASITGYYGRKK"
     gene            complement(1980..2576)
                     /locus_tag="P5573_00510"
     CDS             complement(1980..2576)
                     /locus_tag="P5573_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT26579.1"
                     /translation="MKRTVVLGMMFLLSLAIASVSAAENFSADVIHRGKGYTGQSKVY
                     VQGNKIRLEPKGQSQYSIVRSDLNKTWVVMPDQKMYMESKYDPSQVPVQKRVRGEMSR
                     KLVGSETIDGHPAQKYEVTYRDGGKVMKSYQWVATDINFPVKTAATDGSWSMEYRNIR
                     MGTQPESLFEIPKGYTTMTVPAYPGGMMPGKGKKPYQP"
     gene            3153..3797
                     /locus_tag="P5573_00515"
     CDS             3153..3797
                     /locus_tag="P5573_00515"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HRT26580.1"
                     /translation="MMKKLIIIGILTVLLIPGSVLALTYDFNGIREGRYDQGTFNSLL
                     GPDHYVEFSGGTFVADFYATEESLRLPRSMVIRTYYTGTEDFGATRAAFANPTDFVSV
                     DMGDFGGDEDRLFLNAYDARGNLLDSANSVLGAGVAGSLTLSVSAANIAYVLFGSTTT
                     GDYPNSIYWDNFTFNERPGNGGGNGTAVPEPATMLLLGAGLLGLAGFSRRKHRS"
     gene            complement(3921..4328)
                     /locus_tag="P5573_00520"
     CDS             complement(3921..4328)
                     /locus_tag="P5573_00520"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HRT26581.1"
                     /translation="MYFEEIEIGKVYKSTVTKPITGTEIDLVAQMSGMDLPGFLSAEF
                     AKSKGFKDRVTPGIYVIASMMGLMAKQGFLADAIWTGATDISFRTPAFPGDRLSAEVE
                     VISKKEGKRGGFVTYKWTINNQEGKLVAQGVNT"
     gene            4937..5560
                     /locus_tag="P5573_00525"
     CDS             4937..5560
                     /locus_tag="P5573_00525"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HRT26582.1"
                     /translation="MLCFAAGVMLAISFLQLIPEGINLSSSWVCVLGLITGALLMFGL
                     DRLIPHIHPRLCSQEQGCNLERTSVYLIIGMFFHNFPEGMAIAAGGIVDAEVSLVIAL
                     AIAIHGIPEGVCTSAPFFHVTGERLKAFLVSSSTAIPIVAGYFVARYVFKTISPSWIG
                     FTIGATAGLMIYITVDELIPNACVGQNHLTIFSLLTGIVLVLLLGSL"
     gene            complement(5921..6280)
                     /locus_tag="P5573_00530"
     CDS             complement(5921..6280)
                     /locus_tag="P5573_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT26583.1"
                     /translation="MRKRILKITALPTMIIMVGCVVYGPVSPPPPPPEPVYSHRPATL
                     ERSWNAALDAMEDSDVSIVSADRESGIIRGVRGSADATVTVRTQADGRVRVGFSARGD
                     EGLADQIYRAYERRMGR"
     gene            complement(6298..6603)
                     /locus_tag="P5573_00535"
     CDS             complement(6298..6603)
                     /locus_tag="P5573_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT26584.1"
                     /translation="MKISDWLDEKEADGIDVSQIELPDDLLYDAEPNEVVFFEVYRPC
                     SILCAKNHPFATVERFKHWYCCRGQDKKAGIHTTGMEWRFITKDKDLAIRTARSHME"
     gene            complement(6613..6972)
                     /locus_tag="P5573_00540"
     CDS             complement(6613..6972)
                     /locus_tag="P5573_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT26585.1"
                     /translation="MSRLKLVLSALILVMLAAPPLHAETSSCRTVSGGKLNCMEFAET
                     LKPGLAKVCGIIGPNSKWVKAPCPRKNAPGACRVTRSDGIEQPALCYRMTGLPDGQRI
                     DFCRKSCKGTFVLFKAK"
     gene            complement(7083..7652)
                     /locus_tag="P5573_00545"
     CDS             complement(7083..7652)
                     /locus_tag="P5573_00545"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRT26586.1"
                     /translation="MKKLFLILLTMFLITSLAIAGDQGKGNPTIVIKTNKGVIDAVLF
                     RDRAPITVENFIRYAEAKHYDGTVFHRVIRNFMIQGGGFDQKFSEKLTRAPIKNEATN
                     GLKNERGTLAMARTSAVDSATAQFFINLKDNVFLDHGVRDFGYAVFGKVTKGMDVVDA
                     ISSVPTGPKGPFPSDCPREAVIIESIRVK"
     gene            complement(7744..8694)
                     /locus_tag="P5573_00550"
     CDS             complement(7744..8694)
                     /locus_tag="P5573_00550"
                     /inference="COORDINATES: protein motif:HMM:NF013964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HRT26587.1"
                     /translation="MVSNATGGTHADPEGIRRKVEILIRNFEKELRDGELRQKVLAII
                     PIFRTLRTLGKSLIPVEYASAARDRILYYFRRYPRLTIHGDELLVVSGIQEYARRVRE
                     LRVQFGWAIASGMTIKEMLADEADDHGEGLTDIKPNEYVLLHDEEDRDAAHRWNLANT
                     IRKKRAAVRDKILEYFRMNVGRNITGEELRYVAGNKTEWARRVRELRTEYGWPIASKT
                     TGRPDLAVGVYLLQADRQSPEHDRSIPDDVRREVLRRDGYKCKRCGWSHDEWNPSDPR
                     HLELHHIKHHVHGGENIEANLKTLCSVCHDKQHRNDKIKK"
     gene            complement(9099..10298)
                     /locus_tag="P5573_00555"
     CDS             complement(9099..10298)
                     /locus_tag="P5573_00555"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF012372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HRT26588.1"
                     /translation="MKKELNQPWLLREEQRTYDARMSDKITKKRERFRLIDVFAGAGG
                     MTLGFTSRFGHTFDPVWGNDFDNACVETYNANFGHHCVPGDIVDILNDPKTKIPRAEV
                     VIGGPPCQGFSLLNKQREGDPRKQLWRPYLEVIERSRAKIFVMENVPQLLGSFEHGEI
                     MGMAEKMGFKVWGDLLCAADYGVPQTRKRAFIIGCKFLDPKLVFPPRRSHFNPTTTTL
                     QMDFFQSNAGYLPNAKRWRTVRDVIADLPPPTGTGIRDVPPPLDLHFGRTPTPKSLAR
                     YRAIPKEGMNRFDLQKRALDLTPDCWIRKKSGGTDLFGRLWWDRPAFTIRTEFFKPEK
                     GRYLHPEQHRPITHREAARLQSFPDDFIFKGSKIEIARQIGNAVPPLLAARVADVVYM
                     LLRIGES"
     gene            complement(10679..>11922)
                     /locus_tag="P5573_00560"
     CDS             complement(10679..>11922)
                     /locus_tag="P5573_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HRT26589.1"
                     /translation="TQRPDENLITHIGKLKKGTLRVGDSVHLKVETQTRRATEANHSA
                     THLLNAALHSVLGDHVKQAGSLVNPERLRFDFTHFSRIDEHELDRIETLANEYIQANL
                     PVKTSILDKEAAMKTGAHAVFDEKYSDKVRVVQMGKVSAELCGGTHVTRTGNIGLLKI
                     LSESAVAAGVRRIEAVTGREAVKYIKKVDHELKKVANIFKTGPMEVADRVDKVLKHQK
                     DLEREIEALKGKLAARESADIIKKARSVDGIQVLATTVEASDVKSLRDFGDKIRDKIK
                     SGVILIGSKVGDKAMLLCMVTKDLTAKYDAGRIVKQIAPIVGGSGGGRPDMAQAGGTK
                     PEYLDKAIAKLDAMFKVEAKPAEKPAPVVKTVAKKPTAKPVKAKAARPKAKPKAAKAK
                     SAKPKTKAKAKPKAKAKKKRR"
BASE COUNT         2432 a         3188 c         3073 g         3229 t
ORIGIN      
        1 accgtcgcgg ctttcgccat agcgtcggta aggttccctc cggagaaatg ggtcttggcg
       61 tcgtcccaca tcttgacggc cgcctcatag ccggacttcg cgccctcgag cttcgccttg
      121 tccaggttcg cgggcagttt cttgctcttc gagaggatgt ccagccggct cttgacggca
      181 tcaagcatct tgggcagccc tgcgctcagt tcctcccagc ccttggtcag ttccgccttc
      241 ttggttgcgg ccgccgttgc cagctctttc accttgtccg ggatggcctt tgccgtgttg
      301 agagccgtat cgtagtcttt cttgtcaaag gatcctttcg cagccgtgat cgcgtcctcc
      361 acggccttca ccttgtcggg aacatacttc atggcttcgc ccttgacggc gttcagggca
      421 tcctcagcgg tcttgatggc tgcggctgca ggttccttgt tgctcgcaca ggacataaga
      481 gcaataccga gggccacaca taaaacagct acgagaattc ttttcgactt cattatgacg
      541 tcctcctttc agttagggaa ttcgcagttt ctgtccgacc ttgatcaaat cggggttctt
      601 gagaatgtcc ttatttgctt cgaagatctt catgtacagg ttggggtcgc catagtactt
      661 cttggaaatc tttgacaggg tttccccctt ctggaccacg tgccattgcg tggcatccca
      721 cgcatcggcg ccgccgccct tcggtttttc ttcaccagcc atacgctatt cctccttttc
      781 gctgtcccga tgttttttcg tcgctgatac tcctgacgcg atatcacgtt ccgggaacgg
      841 cccccggttt atgcttttga tccctgaaaa aaacacccag acgtgtgccc gcgaaagagg
      901 caaccatcca gatcatagca ctcagaagga aaaagaataa gaaaaatcga ttgagtgtcc
      961 gcgcctgcag taacgtgggg accaggaaga caagcgccgc gtagaagccc gggctgaccc
     1021 atagaaaatc cattgccgac cttttctcca gaaggggctg gaccagatag cccgacacaa
     1081 aactgccgat gaggaagcaa accgggaaaa tcaccatgaa agcgatgccc atgtaggatt
     1141 caggtgcctc cccgcttgat tgaatcctcg ggttgatggc gaggaataga atgatcgagt
     1201 agaaaagcag ggtgacgccg gcacccgtga tgacatacag gaacttctgt ttcattctct
     1261 gttctctcga ggttcatgaa cagcggggag aaacagcatt tccgaatcct cgcccgcgga
     1321 ttccagcagg tatccgaatg cgcgcttatt tcttgcgtcc gtaatagccg gtgatcgagg
     1381 ccttcccgat ggcattctgg ttgatgaaaa acccggtcat ggccgccgtt gcgttcggcg
     1441 gcagatcgat tttctcgacc ttcaatgcgt gaatggtgaa gatgtaacgg tgcggcttgt
     1501 ggccgaccgg ggggcagggg ccgccgtatc ccgggccccc gaaatccgta ttggtctgga
     1561 ggctgcccgc tggaagaagt ttcccgtccg gtgtgcccgc gcctttgggc agcgacgtca
     1621 cggtggcggg gatgttgtag accacccagt gccaccagcc gcttcccgtc ggtgcgtcgg
     1681 ggtcgtaaac ggtcagggca aagctcttcg tgcctgccgg tgcgtttttc caggcgaggg
     1741 caggggagag gttcttccct gagcacccga acgagttgta gacctgatcg ttctgaaggg
     1801 ttgccctgtg tttgaagtcg gggctggtca acgtgaacgt ttcagccccc agggccggtg
     1861 agacgatgca gagaataaaa ataaagcata gaaaatatcg catgggacat acctcctttg
     1921 tctgacagaa tcttttcaat gcgtctgggg ccgcgttttc aagagagccc ctcgatccgt
     1981 catggctggt aaggtttttt cccctttccc ggcatcatgc cgccggggta cgccggcacg
     2041 gtcatcgtcg tatagccttt gggaatttcg aagagactct ccggttgtgt ccccatgcgg
     2101 atgttccgat actccatgct ccagctcccg tctgtggccg cggtcttgac ggggaagttg
     2161 atgtctgttg ccacccactg ataggacttc atcaccttgc cgccatccct gtatgtgact
     2221 tcgtattttt gcgcgggatg gccgtcgatg gtttcggacc ctaccagttt tctggacatc
     2281 tctccccgca ccctcttctg gacgggaacc tgtgaggggt cgtacttgga ttccatgtac
     2341 atcttctggt ccggcataac aacccaggtc ttgttgagat cgctgcggac gatgctgtat
     2401 tggctctgcc ccttcggttc cagacgtatc ttattcccct gcacgtaaac cttcgactgc
     2461 cccgtgtatc ccttgcccct gtggatgacg tccgccgaga aattctccgc ggctgaaaca
     2521 gaggctattg cgagactcag taaaaacatc atccccagca cgactgtcct tttcattggc
     2581 catctccgtt tcatgttttt tgaatctgtt ctgtgaatca tgtttcgccc cgatgcccct
     2641 tgagttgagc cgcagatgtc gatagactca tggagggggg aaatcggcta ttataatgac
     2701 attttgatat ggtgattggc aactatttta atcggagttc cgggcatccg gcaatacccg
     2761 cgggtacccc tgatttttct attaaaaaaa gtggcccgtc acttttttct cttgagcccg
     2821 gaaatgcctc tgatttgtct ttcgaaaggc ctttgccgtc tttttttgtc aaaatgtcaa
     2881 gatatcaagc ctgacccctt ttttttaaat taagacggta ggttatcata cttgagcgag
     2941 gggggtgagt cgtggttgac ggggaggatt agagccgtct aatacttttt tattacctca
     3001 aaatggtact ttgtactgct tgacttttgt ctttttttag ggcatcaaag gatacagagt
     3061 atcggacttg tgataatcaa gggaatgctg tttcttgttg cgaattgccg ttcaggattg
     3121 actggcaaca gcggagttta aaggggggct gaatgatgaa aaagttgatt ataataggta
     3181 tcctcacggt cctgctgatc ccgggttcag ttcttgcgct gacttacgat tttaatggga
     3241 taagggaagg tagatatgat caagggacct ttaattcgct acttgggccg gatcactatg
     3301 tagaattctc gggaggtaca tttgttgcgg acttctatgc tacagaagaa agcttaaggc
     3361 ttccccgttc catggtaata cgaacttatt atacgggaac ggaggatttc ggtgcgacaa
     3421 gggccgcatt tgccaatccg accgactttg tcagtgtcga tatgggcgat tttggtggag
     3481 atgaggatcg gcttttcctg aatgcatatg atgctcgggg caacttgctt gacagtgcca
     3541 attcagtcct tggggcaggt gtagctggaa gtctaaccct gagcgtttcg gctgcgaaca
     3601 tcgcatatgt gcttttcggt agcacaacaa ccggggacta tcctaacagc atctactggg
     3661 acaatttcac tttcaacgaa cgccctggta acggcggcgg caatggtact gcggtgcccg
     3721 aacccgccac aatgcttctt ctcggcgccg gtcttttagg cctggccggg ttcagccggc
     3781 gtaaacatcg ctcttaactt cagatgtctg agcagcatct gaaacctcaa agaaagggct
     3841 gatcttttcg gagatcagcc cttccgtttc gtaagactgt cttctaatca tcctccagat
     3901 gattatttgc ccgggaacag tcaggtgttc accccctggg cgacaagctt cccttcctgg
     3961 ttgttgatgg tccatttgta ggtaacgaac ccgcctcgct ttccttcctt cttgcttatg
     4021 acctcgacct cggcggagag gcggtcgccg gggaacgcgg gcgtcctgaa ggaaatgtcc
     4081 gtcgccccgg tccagatggc gtccgccaaa aagccctgtt tggccatgag acccatcatg
     4141 ctcgcgatga cgtagatgcc gggggtgacc ctgtccttga aacccttgct cttcgcgaat
     4201 tcggcactga ggaagccggg caggtccatg ccggacatct gcgccacaag gtcgatttcc
     4261 gttcccgtga tgggctttgt tactgttgat ttgtagactt tgccgatttc aatttcttca
     4321 aaatacatgt tttgacctcc tttcgttgtg aagcctgttt gcgctgcttt ccccttcggc
     4381 atttactgat aaaatatcgt atagccgaag tcaagaaata cccatctgtc ggacctgccg
     4441 actcacttca cgaaacaggt gaaataaagg ggacagtccc cttttccggc ccaggttgtt
     4501 gatatggtaa atccggctgc tgcctgtttc aaaacagcct gggcttttga tcccccgatc
     4561 catcttaaat ccgcatttcg tcaggcacag cgcggttttc gatgtttatt ctttattatt
     4621 ttcaggtatt tgtgtataag aaaacggaca tgcttcaaat gtcatttttc atgttagatt
     4681 taaaatgtct cttcggggat acatgcagag atcctgtcgg gcacagataa aaacctgctt
     4741 cattctttga aatggagccg atggaatctc ttcggggtgt ggcatcttta ttccgacaca
     4801 gtgcccctct tttcaaagaa aaggactcct tgccatggac atgactgcgt ggatcatcgt
     4861 gatttccttc gctggtccga ttataggttc ggcaatcggt gtttccagac gaccatcctt
     4921 tccatacata tgcaacatgc tctgttttgc agccggggtc atgcttgcca tttcgtttct
     4981 ccaactcatc cccgagggca taaacctgtc ctcatcctgg gtctgtgtgc tcggtctcat
     5041 caccggggcg cttctcatgt tcggcctgga tcggctgatc ccgcacatcc atccccgtct
     5101 gtgcagtcag gagcagggat gcaacctgga acggacctcc gtgtatttga ttatcgggat
     5161 gttttttcac aactttcctg aggggatggc cattgccgcg ggcgggattg ttgacgcgga
     5221 ggtttccctg gtgatcgcgc tggccatcgc tatccacgga atccccgagg gcgtctgcac
     5281 atccgctcct ttttttcacg tcaccggcga gcgcttgaag gcttttcttg tctcctcgtc
     5341 gaccgcgata cccatcgtgg cgggctattt tgtcgcccga tacgttttca aaaccatctc
     5401 accgtcatgg atcgggttca ccatcggggc cacggccggt ctgatgatct atatcaccgt
     5461 agatgagctg attccgaacg cgtgcgtggg gcaaaatcac ctgaccatct tctcgcttct
     5521 gacggggatc gtgctggtcc tattgctggg ttccctgtaa aaaaacgaac tccctgtgcg
     5581 ggttttggga tcagatcctg ctatttgccg tttccacggg ggagaaaaca aaacgtgcgt
     5641 attcataatg aggtgatggt ttgatgtgat tgatgacaag tttcgagttc ttcagcgtcg
     5701 ttcagatacc gggtgctgaa ttatcgacgg tacgcgcatg ggtaatatga aaaatcttgt
     5761 ctaaatgctc cgcctcgtaa aaaggctctt ttcctttaaa agggtttaac ccacagccgc
     5821 cacgaaagga gaaagcaact cgtgaaactg gagattaccg tggtggaagt cgccgagatt
     5881 ataaagtctc ttccgaatgt caaccctgaa cccggctgtt ttatcggccc atccgccgtt
     5941 cataggcgcg gtagatttga tcggccagtc cctcatcccc cctggcactg aaccccaccc
     6001 ggacccggcc gtcggcctgt gttctcaccg tgacggtggc atctgcggaa cccctgactc
     6061 ctctgataat tccgctttct ctgtcggccg acacaatgct cacatccgag tcttccatcg
     6121 catccagcgc ggcgttccag gaacgctcca aagtggccgg cctgtgggag taaaccggtt
     6181 caggcggggg cggcggagga gacacagggc cgtatacgac gcaaccgacc ataatgatca
     6241 tggtcggcaa cgcggtgatt ttcagtatcc gtttcctcat gtctatctcc cgatcagtca
     6301 ttccatgtgt gatctggctg tccggatggc gagatccttg tcctttgtga taaatctcca
     6361 ctccatgccc gtcgtgtgaa tgccggcttt cttgtcctgg cccctgcagc agtaccagtg
     6421 cttgaagcgc tcgaccgtgg cgaaagggtg gttcttggcg cagagtatgc tgcagggcct
     6481 gtacacttca aagaacacaa cctcattggg ttcggcgtcg taaagcagat cgtcgggtaa
     6541 ctcgatctgc gatacgtcaa tgccatcggc ctctttttca tccagccagt ctgagatttt
     6601 catgggatgt ctttacttgg ccttgaagag aacgaaagta cctttgcaac tcttcctgca
     6661 gaagtcgatg cgctgcccgt cgggcagacc cgtcatccgg tagcagaggg ccggctgctc
     6721 gatgccgtcg ctccgtgtca cccggcaggc acccggtgcg tttttcctgg ggcagggggc
     6781 tttcacccac ttcgagttcg gcccgatgat gccgcagacc tttgcgagac cgggtttcag
     6841 cgtctctgcg aattccatgc aattgagctt gccgccggaa accgtccggc aggatgacgt
     6901 ctcggcgtga agcggcggtg ccgcgagcat caccaggatc agggcggaca gcacaagttt
     6961 cagcctgctc aaaaaacacc ttctcgtaag tggcaagatc gttacgtgat gggttaacca
     7021 gactgaatgg aaaatagccg attcacaatc ttgaaatgca ggactccccc cacgaatccg
     7081 gactacttga cccgaatcga ctcgatgatc acagcctcgc gagggcagtc cgaggggaac
     7141 ggtcctttcg gcccggttgg cacgctggag atggcatcaa ccacgtccat gcccttggtg
     7201 accttgccga agacggcgta gccgaaatcg cggacgccgt ggtcaaggaa gacattgtcc
     7261 ttgaggttga taaaaaactg cgcggtggcc gaatccaccg ccgaggtgcg ggccatggcc
     7321 agcgtgcccc gctcgttctt caggccgttt gtagcctcgt tcttgatggg cgcgcgggta
     7381 agcttctcgg agaatttctg gtcgaaacca ccgccctgga tcatgaagtt cctgatcacc
     7441 cggtggaaga ccgtcccgtc gtagtgtttg gcctcggcgt acctgatgaa gttctcgacc
     7501 gtgatgggtg ccctgtccct gaagagaaca gcatcgatca cgcccttgtt ggtcttgatt
     7561 acgatggtcg ggttgccctt gccctggtct ccggcaatcg ccaggctcgt gatcagaaac
     7621 atcgtcaata agatcagaaa caattttttc atgcattcac tccttttagc gagtaatgat
     7681 catgcactac ttttatattg tacggcgtat attccctgag ttttatacgt aaaccggtat
     7741 tttttatttc ttgattttat cattccggtg ttgcttgtca tgacatactg aacaaagtgt
     7801 tttcaaatta gcttctatgt tttcgccgcc atggacgtgg tgttttatgt gatgcaactc
     7861 taagtgtcga ggatctgatg gattccattc atcgtgcgac caaccacaac gtttgcattt
     7921 atatccgtct ctcctgagta cttcacgccg cacatcgtct gggatgctcc gatcatgttc
     7981 gggactttgc cgatctgcct gcaggagata tacacccaca gccaggtctg gacggccggt
     8041 cgttttcgac gcaataggcc agccgtattc tgttcgaagc tctcgtacac gccgagccca
     8101 ttctgtctta ttgcctgcaa cgtacctcaa ttcttcgccc gtaatatttc gaccgacatt
     8161 cattcgaaaa tactctaaaa tcttgtctct tacggcggct cgctttttac gtatcgtatt
     8221 tgccaaattc caacgatgcg ctgcgtcacg atcctcctca tcgtgaagaa gcacatattc
     8281 gttcggcttg atatccgtca atccctcacc atggtcgtcg gcttcatctg ccagcatctc
     8341 tttgattgtc atgccgctgg caatagccca accaaattgg acacgaagct ccctgactcg
     8401 cctagcatat tcttgtatac cggacacgac aagaagctcg tcgccatgga tagtaagacg
     8461 cggatatctc ctgaaatagt aaagaattcg atctcgggca gctgatgcat actctacggg
     8521 aatcaaggat ttgcctagag tccgaagagt tcgaaagatt ggaattatag ctaacacttt
     8581 ttgacgtagc tcaccatccc gcagctcttt ttcaaaattc ctgattaaaa tttcaacttt
     8641 ccgtctgatg ccttcggggt cggcgtgtgt tccacctgtt gcgttactaa ccatttagaa
     8701 acctttccaa tcttttcagc aatttttgag gcttctgtgt ctcgcactgc cagacgacca
     8761 gaactttcca ccccatattc tgcaacttgc ctttaaacga tttgtctcgt tttatgtttt
     8821 gatcaagctt cctattccag aaagctttgt ttgttgaggg gcgcttcgaa cgcgagcaat
     8881 taatatgact atgccaaaaa catccatgaa cgaagatcac ctttcgatga cgtggaagaa
     8941 caatgtcggg gttccctgga aggtcacgac gatgcaatcg gaaccgatag ccaaggcgat
     9001 gtacaaatga ccggactaca aattccggct ttgtgtttct gttcttgact cggctcatta
     9061 tccaacttct ctttgcggga gagaagacat ccgtcatatc aactttcacc gatccttagg
     9121 agcatataga cgacatctgc cacccgtgct gctaaaagtg gcggaacagc gttgccgatt
     9181 tgtcgggcga tttctatttt agatccctta aatataaaat cgtcgggaaa agattgaagt
     9241 ctggcagctt cacgatgtgt gatcggtcga tgttgctcag gatgcaagta tcggcctttt
     9301 tccggtttaa aaaactccgt ccgtatggtg aatgccggtc tatcccacca gagtcttcca
     9361 aaaagatctg ttccgccgct cttcttgcga atccagcaat caggggtcaa atcaagagca
     9421 cgcttctgca gatcgaagcg gttcatacct tctttaggaa tggctctgta tcttgctaga
     9481 ctcttaggtg taggcgttcg tccaaaatgc agatctaagg gtggtgggac gtctctgata
     9541 cccgtgccgg ttggtggcgg caagtctgca attacgtctc ggaccgtacg ccacctttta
     9601 gcgttaggga gatatccggc attgctctgg aaaaagtcca tttgtaacgt cgtcgttgta
     9661 ggattgaagt gagaacgacg tggagggaaa accaactttg gatcaaggaa tttacaacct
     9721 atgatgaatg cacgttttct tgtttgaggt acaccatagt ctgctgcgca gagcagatca
     9781 ccccaaacct tgaagcccat tttttccgcc atacccatga tttcaccgtg ctcgaacgaa
     9841 cccaaaagct gcggcacgtt ttccatgacg aaaattttgg cacgagaacg ctctataacc
     9901 tccaagtaag gtcgccacaa ttgcttgcgt ggatctcctt ctctctgttt gtttaaaaga
     9961 ctgaaaccct gacagggagg accgccgatt acaacttcag cacgtggaat cttggttttg
    10021 ggatcattta atatgtctac aatatcaccc ggaacgcaat gatgaccgaa gtttgcattg
    10081 taggtttcta cgcaggcatt gtcgaaatca ttaccccata ccggatcaaa cgtgtgtccg
    10141 aatcggctcg taaatccaag agtcatccca ccggctcccg caaagacatc aataaggcga
    10201 aagcgctctc ttttctttgt gattttatcg ctcatgcgag catcgtaggt gcgctgctct
    10261 tcccgtagga gccatggttg attaagttct ttcttcattg aaaatgcttt tttttagaaa
    10321 aagaggtcag gcttgacatc ttgacatttc cttgctgact tctcataaac aaaaccccag
    10381 tcctctttgc gagatacggg gttttgttgc catcactcca tgttcccatc ctgtgccgtg
    10441 aagtggcagg tcacagaaga cagcggacaa tgttcgtctc tctggtcttg tatgtttact
    10501 tcagtctttc aaaatccgtt tcagttcggt aaagggtctt tcatggtttg agtccggcgc
    10561 ggcagaccgt tttggtctgg ggcaccaggc ccgacaaata gcatgggtcg ccacgccgcg
    10621 tctctttggt cactctcgtc gtttcgttcc ttgtatcttt tctgatcttc tgaacttctt
    10681 atcttctttt tttcttggcc tttgccttcg gttttgcttt tgccttagtc ttgggtttgg
    10741 ctgatttagc cttcgcagcc ttcggcttcg cttttggcct ggcggctttg gcctttactg
    10801 gtttggccgt cggttttttc gcgacagttt tgacaaccgg agcgggcttc tctgccggtt
    10861 tcgcctcgac cttgaacatg gcgtcgagct tggcgatagc cttgtcaaga tattcgggct
    10921 tggttccgcc ggcctgggcc atgtcgggcc tgccgccgcc gcttccgccc acgatggggg
    10981 cgatctgctt gacgatcctg ccggcgtcgt acttcgcggt gagatccttc gtgaccatgc
    11041 agagaagcat cgccttgtcg cccaccttac ttccgatcag gatgacgccc gacttgatct
    11101 tgtcgcggat cttgtcaccg aaatcgcgca ggctcttgac gtccgaggcc tccacggtgg
    11161 tggcgagaac ctggatgccg tctacgctcc gggccttctt gatgatgtcg gccgattccc
    11221 ttgccgccag cttccctttg agggcctcga tctcgcgctc gagatctttc tggtgcttga
    11281 gcaccttgtc gacacggtcc gccacttcca tggggccggt cttgaaaatg ttggccactt
    11341 tcttcagttc gtgatcgacc ttcttgatat acttgacggc ctcgcggccc gtcacggcct
    11401 cgatcctgcg cacacccgcc gccacggccg actcggagag aattttcagt agaccgatgt
    11461 tccccgtgcg ggtgacgtga gtgccgccgc agagctcggc gctcaccttg cccatctgca
    11521 cgacgcggac cttgtcggag tatttctcat cgaataccgc gtgggcgccc gttttcatcg
    11581 ccgcttcctt gtcgaggatg ctcgttttga ccgggaggtt cgcctggatg tactcgttgg
    11641 cgagtgtctc aatccggtca agttcgtgct cgtcgatgcg cgagaagtgt gtgaagtcga
    11701 agcgcaggcg ctcggggttg acgagcgagc ccgcctgttt cacgtgatcg cccaggacgc
    11761 tgtgaagggc ggcgttcagc aggtgagtcg ccgagtggtt ggcctcggtg gctctacggg
    11821 tctgcgtctc caccttcagg tggaccgagt cgccgacccg gagggtcccc ttcttgagct
    11881 tgccgatgtg ggtgatgagg ttctcgtcgg ggcgctgggt gt
//
LOCUS       DAOTMX010000009        11574 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_5279_length_11574_cov_3.704922, whole genome shotgun sequence.
ACCESSION   DAOTMX010000009 DAOTMX010000000
VERSION     DAOTMX010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 11574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11574
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5279_length_11574_cov_3.704922"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /gene="pgk"
                     /locus_tag="P5573_00565"
     CDS             <1..674
                     /gene="pgk"
                     /locus_tag="P5573_00565"
                     /inference="COORDINATES: protein motif:HMM:NF012389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HRT26590.1"
                     /translation="SKALEKPARPLVAIIGGSKVSGKIDALGHLVKKVNKMILGGAMA
                     FTFLKALGCEVGKSLVEDDFLDKARDIMKTAKELGVKLYLPVDCVVAEEMSAEAETKI
                     VPVQEINSKWMALDIGPATITLFTEALSNAKTIVWNGPMGVFEIDAFSRGTSSLAHIV
                     ANTYALSIVGGGDTDVAIAKAGERDNMSYISTGGGAFLELLEGKDLPAVKALKTCGQL
                     LQGRQ"
     gene            749..1489
                     /gene="truA"
                     /locus_tag="P5573_00570"
     CDS             749..1489
                     /gene="truA"
                     /locus_tag="P5573_00570"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HRT26591.1"
                     /translation="MKNLKMVLEYDGTAYYGWQRQVGVRTIQETVEGRILLMTRERVT
                     LIGSGRTDAGVHALRQVANFRTTTGLAERTLLRGLNSLLPKDIAVKEITEVDEEFHSQ
                     YDAKSKVYEYRIFNSPTRTALLRNYCWHVYGHLDLEAMKEAAGMLPGRRDFSSFCASG
                     HESKSMVREVFDCRLESDCKGMVFFSVEADGFLRYMVRNIVGTLVDVGKSKLSPEGFR
                     QVMKAKDRRKAGVTAPPNGLFLVDVKYT"
     gene            1486..2382
                     /gene="rfbD"
                     /locus_tag="P5573_00575"
     CDS             1486..2382
                     /gene="rfbD"
                     /locus_tag="P5573_00575"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:TIGR01214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HRT26592.1"
                     /translation="MKILILGHKGMLGSELFSRFSADHEVVGKDIDEFDITSAYDCRD
                     VIDEVAPDCVINAAAYTDVDGCETDRDLCFAVNAQGVRNVAEACGDSVCIVHFSTDYV
                     FDGTKKEPYKENDPTNPLNVYGQSKLDGERYLREMTDNYVLVRTAWLYGKNGRNFVRT
                     ILEKAGNSDVLEVVADQVGSPTYARDLAAAVQVLVEGGHRGLYHLTNRGRCSWYEFAL
                     KIIEFADLGNRIEVRPITSDRLIRKAMRPAWSVMSTMKFSNLTGKTLRFWQVALKEFI
                     DENKAWLTAKSTETSHGVVKQS"
     gene            2379..3506
                     /locus_tag="P5573_00580"
     CDS             2379..3506
                     /locus_tag="P5573_00580"
                     /inference="COORDINATES: protein motif:HMM:NF027862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein T N-terminal
                     domain-containing protein"
                     /protein_id="HRT26593.1"
                     /translation="MKKRKTLLQIVVVGLCLAFAGGSLFAQTRIEAQGVATIHNNMVD
                     VARDKAIDNAQREAVEKAVGVMVSSFTEVENYQVKIDQILSESRGFINTYKVLDEKRE
                     DNQYRVTIEADVSVNRLKDKLEAVNLILSRKSRPRLMVLIVQGEKRDEVAESAMIKHF
                     LAQGFKLVETKDVNRELGRYNLTVVADDAAAASKLGSRYGAEVLVVGRTEASTSRFTV
                     NGVEMRFNKVDLSARAVKVDTAEVIATDSEIQSGPGMEDALKSMTVEASGKLARKLTD
                     QLVNRWSYELTNTTTIKLIASGLRSYRDLVKFKELITNEVKGVKEVNQREYRNGHVEI
                     DLEVRGNVHGVADDIAALRINGKAVRVQGISQNTIKVRVGP"
     gene            3539..5530
                     /locus_tag="P5573_00585"
     CDS             3539..5530
                     /locus_tag="P5573_00585"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HRT26594.1"
                     /translation="MGRLSISKCLAVFFCVAFVVGCAGGESFKRGEQLAAERRWDEAI
                     EFYQKALEENPSSKEYKESLEKARQESAKIHFEKAKQLMPAEKQADITRLEQVVKEAD
                     LAYKLDPQNKAIAAFYEDVNRKKSELQSLVKTLYSQADADLKKDDWLAAIQKLNRINE
                     IFPGYEDTGDKLAQAVQQASGAYYKRGIDLEKQEDWRGATKAFKAVVDINPGYLDAAK
                     RYEEARSRDNVGYCIKAGNAAAQAMDWQRAVMFFERALEYDPSNREVAGQLEKLKEKA
                     AKSLFDQAIKDVQRGKIYQGAKKLELAKTYLPSLQEDTLFKEFVAGNLGVKLVDRAEQ
                     YAEKGKWGNALIALQQLEALDPNYKGLFFKIQEAKDNIKKRIRKSIAIFDFKSPSSHK
                     DAGRIVANKLMTFLYKNASGDIRIIERENLESILKELQLGQTGLVDVDTMKALSKMGG
                     IDTFVMGDVLRFTSEYKDFPSMNQVKVVIDEEEVRNPEYVEWLILHKNPSEEDLKTAP
                     PKTVTRKNHQLISYKTGYAKISASIEVSYKLVDARTGENLFTNTVAGRLSKEDKYQDA
                     VPLAGINGDPLELPTETDVLDELTNAKVSEIGQSVLRHFSSLEVEYNNQALLQIKRRN
                     RDEAVERFMDAIFDENMKGISTPVSQSAKEMIDKLTEDM"
     gene            5609..7576
                     /locus_tag="P5573_00590"
     CDS             5609..7576
                     /locus_tag="P5573_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT26595.1"
                     /translation="MVAMCVALAAVLAFPTGSVAQARTVKLSVFNFGASNIDASSYAT
                     MVTNYLISSFRGNPALSVLDRKELEYFLFLNDLQQNDATENVIHIGSRLALDFVVAGT
                     VGKTGSAITVRCKLFSVSQKRVLQSFLIRAYGDAELMTETYKLGEQIAKLLSKEAESR
                     KDESAGLPSPPVNVEARPGNMSVYLRWEDPPGESPAGYKVYRGLSESGPFVQIAQLPR
                     REYLDQGLEKKTTYYYRLKSFDGSGIQSSESPVVKVETVPTPLAPIILKAEGHVKSVI
                     LTWCVNPRKSDDPLPLKGFKVYRAESADGPYTDTHKVTLEEIGITSPDNVSAEVKIAV
                     KCPVDGKEYFFKLTAYNENNLESNFSEPVRASSIPGVQKISAQGGLIREVRLSWVAVD
                     SPFVKGYWIYRSVGDEVDFVRLRKVEVSYSTGTINYSDVEGLADETRYNYRVTAFEYP
                     QTETDPSPVVSAVTKGKPPVPRDVKAISGLARRVQVVWTPNTEDDVEGYKLYRSKTKD
                     GRFEFVKKVPGRNSQGVVDEGGLFGKLEDQAEYHYILRTYNKVDVESDDSAVVSAMTK
                     AKPQKPTGLKWETQGKKVILTWSKNPEPEIVTYRVYEIMSSGIKEIGRVKDTRFSVDA
                     PGSGTSKRYVVTAVDKDDIESEHSDEVTVEK"
     gene            7748..8059
                     /locus_tag="P5573_00595"
     CDS             7748..8059
                     /locus_tag="P5573_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT26596.1"
                     /translation="MRGHLLKGMVVVIVGFLLCMVVPDAAMAQACGKFQVVAGADDNA
                     YLVNTETGAVWVLTHRTLVSGTGREPVAIPYKFLYLLPEHNAVLDEGGQKVPVKERKS
                     R"
     gene            8087..8887
                     /locus_tag="P5573_00600"
     CDS             8087..8887
                     /locus_tag="P5573_00600"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRT26597.1"
                     /translation="MVCPVPACGFSRTYVITGDTGLAVVDVGSVGAADDVMEFIKRSL
                     GGDVKDVRAILATHFHFDHIGGIGRLLFSAAADTKVFFHRLVKDYLEGRNVLAPMKNW
                     CTAFGPTAWKSAGYVRKPSHLHFESLAGIPLPALRKLSTLPYPMDRIVFFDGDGARRY
                     LAGFDGWEVIETPGHTEDSLSFYHGGSEELICGDLMVNMDKGGKGRLNGFYASKEGVV
                     ESFDFLLRNVKAKTIYPGHGEPVQSEGNALRGVEAFAVGRDDALKDIG"
     gene            9083..>11574
                     /locus_tag="P5573_00605"
     CDS             9083..>11574
                     /locus_tag="P5573_00605"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF799 family lipoprotein"
                     /protein_id="HRT26598.1"
                     /translation="MTSLFKGTYKVDPYMEDHKPRTVAVLPFIDKSGSVEGSQEVRKS
                     FYNHFSSLPFKDMELYRVDRLLAKAGLTDAAEINKKTPQELGKILEVDAVVYGEISNF
                     DKFFAGIYSQVSVGAEVRMYETKTGHFLWSGQHVARIHEGGIATTPVGIIATVIAAAM
                     NMRDIQLLRACDDLFRDMVKTIPTPTITEAMRPPVITLLTQDTKGLPKKAGDEIRVVI
                     QGTPKMQATFDIGDYRKNIDMQEVEPGGYLGVYRVVPGDNVENAIITGHLTDDSGNRT
                     DWVDAIGFVTLDTTPPQKPKGLSGVGRDKHNLLRWEKNTEPDLTGYRLYRSQTPLTGF
                     VEVAKTELTEAKDVDLVNSQKYYYRVSALDRAGNESEKSEAVCVMPVAPGPTAVSGAI
                     VADTTWYSGASPYVIEDTVTVVDKAVLTVEPGTEIRSKGKGIVVEGRIMALGDRESLI
                     TFSGMDGTRWEGISFRNVREKENVIRFARVRNALTGVTCESSSPRIEDSEFIDNTEAV
                     KISGAFSKPSLSRNTLQRNETGVTIAEGAQPYLAENLIRDNAKGGIVASNAAPSIQRN
                     TVIQNQGSGVVVRSSQAAVAENNIFDNTPFDMVGSSSGEPVKAHNNWWGTAQGFEVFK
                     KVQGRVDVAKILDGPYPGGKPLELPVLSSNLGGPLTGDGYLVLSTSPYRVTKDVIVDG
                     GHVLYIQSGVQVLYDQKTSIVVKDGGVVAKGTRERPILFSAAAASPVPGFYAHALQFA
                     APTKVNSFLEYAIVKYADTAIDVQHGSPDISYSYIAENAQAGLFCGNDASPKITFNTF
                     YGNQGQGAIACRGMSRPKINHNNFVGNAFAMQA"
BASE COUNT         2886 a         2841 c         3526 g         2321 t
ORIGIN      
        1 tcagtaaagc cctggaaaag cccgcgaggc ccctggtcgc catcatcggc ggttcgaagg
       61 tgtcggggaa gatcgatgcc ctgggccatc tggtgaagaa ggtgaacaag atgatcctgg
      121 gaggtgccat ggccttcact tttctgaagg ccctgggttg tgaggtaggg aagtctctgg
      181 tggaagacga cttcctggac aaggcccggg acatcatgaa gaccgcaaaa gaactgggtg
      241 taaaattgta ccttccggta gattgcgtcg ttgcggaaga aatgagcgct gaagcggaga
      301 caaaaatcgt ccctgtgcag gaaataaatt ccaagtggat ggccctcgat ataggaccgg
      361 caactatcac gctgtttaca gaagccctca gcaacgcgaa gaccatcgtc tggaacggac
      421 cgatgggggt tttcgagatc gacgccttca gccgtgggac gtcatccctg gcgcatatcg
      481 tagccaatac gtacgccctt tcaatagtcg gtggtggcga taccgatgtg gcgatagcga
      541 aggccgggga acgcgacaac atgtcctaca tctcgacggg aggaggcgcc ttcctggaac
      601 tgctggaggg taaggacctc cccgcagtaa aggcattgaa gacttgcggg cagttgttgc
      661 aaggtcggca atgagattgt cgttgtattc cgttatctgc atacgggcac acgctggttc
      721 ggcgaattcc atggttttcg aacattagat gaagaacctc aagatggtcc ttgagtatga
      781 cggtacggct tactacggat ggcagcggca agtcggtgtt aggaccattc aagagacggt
      841 cgaaggtaga atactgctga tgacccgtga gcgcgttacg ctcatcggtt cgggaaggac
      901 cgatgccggg gtgcatgccc tccggcaggt ggcgaacttt cgaacgacga cgggcctagc
      961 ggaaagaacg ctcctgcgag gtctgaacag ccttctccct aaggacatcg ccgtcaaaga
     1021 aatcacagaa gtggacgaag agtttcattc ccagtacgac gcgaagagca aggtttacga
     1081 gtacaggatc ttcaacagcc ctacccggac ggcactgttg cggaactact gctggcatgt
     1141 ttacggtcat ctcgacctgg aggccatgaa agaggctgcg gggatgctgc cgggaaggcg
     1201 cgacttttcc tctttctgcg cctccggcca cgaatcgaag agtatggtgc gagaagtttt
     1261 cgactgccgc ctcgagtccg actgcaaggg catggtcttt ttttcggtcg aggcggacgg
     1321 gtttttgaga tacatggtca ggaacatagt cggaaccctt gttgatgtgg gtaagtcaaa
     1381 gctttctccc gaggggttca gacaggtgat gaaagcaaaa gaccggcgca aagcgggtgt
     1441 tacggcccct ccgaacggac tttttctggt agacgtgaag tacacatgaa gatccttatc
     1501 ctcggccaca aaggcatgtt gggaagcgag cttttctcga ggttctctgc cgatcatgag
     1561 gtcgtaggta aggacatcga cgaatttgat atcacctcag cctatgattg ccgggacgtg
     1621 atcgatgaag tggcgcctga ctgtgtgatc aacgccgcag catacacaga tgtggacgga
     1681 tgcgaaacgg accgggacct gtgttttgcg gtgaatgcgc agggcgtgag gaacgtagcg
     1741 gaagcctgtg gcgactctgt ttgtatcgtc catttcagca ccgattacgt cttcgacggg
     1801 acgaaaaaag aaccctataa ggagaatgac cccaccaacc ccctcaatgt ttacggccaa
     1861 tccaagctcg acggtgagcg ttatctgcgc gaaatgaccg ataattatgt cttggtgagg
     1921 acggcctggt tgtacggcaa gaatggcagg aacttcgtca ggaccatcct ggaaaaagcg
     1981 ggaaactcgg atgtgctgga agtagttgcc gaccaggtcg gttctccaac gtacgcgcgt
     2041 gaccttgccg ccgccgtcca ggttcttgtg gaaggaggcc acaggggtct ctaccatctg
     2101 acgaaccgcg gaagatgcag ctggtatgag tttgcgctga agataatcga gttcgccgac
     2161 ttggggaaca ggatagaagt caggccgata acgtccgacc ggttgataag aaaggccatg
     2221 agaccggcgt ggtccgtcat gagcacgatg aagttctcga acctgacggg caagacattg
     2281 cggttctggc aggtggcgct gaaggaattc atcgacgaga acaaggcctg gctgacggca
     2341 aaaagcaccg agacctccca tggggtggtg aaacaatcat gaagaaaagg aagacgctct
     2401 tacaaatcgt tgtggtcggc ctctgtcttg cttttgctgg cggcagtctt ttcgctcaaa
     2461 cgaggattga ggctcagggt gtggctacaa tccacaacaa catggtcgat gtcgcccgcg
     2521 acaaggccat cgacaacgcc cagagggaag ccgttgagaa ggcggttggc gtcatggtgt
     2581 cgagctttac cgaggtagag aattatcagg tcaaaatcga ccagatactg tccgagtccc
     2641 gtggtttcat caacacgtac aaggtccttg atgaaaaaag agaggataac cagtaccgtg
     2701 tcacgatcga agccgacgtc agcgtaaacc ggttgaagga caaactggaa gcggtcaacc
     2761 tgatcctgtc gcgcaagtcc cgacccaggc tgatggttct gatagtccag ggagagaaga
     2821 gggacgaggt tgcggagtcg gccatgataa agcatttcct cgcccaggga ttcaagttgg
     2881 tggagaccaa agatgtcaat cgggaacttg gtaggtataa tctcacggtc gttgccgatg
     2941 atgcggccgc cgcctcgaaa ctgggaagcc gttacggcgc cgaggtcctg gtggtcggga
     3001 ggaccgaggc ctcgacaagt cggttcaccg tcaacggggt cgagatgagg ttcaacaagg
     3061 tggacctgtc cgcgagggca gtgaaggttg atacggccga ggttatagcc acggacagtg
     3121 agatccagtc cggtccgggc atggaagatg cgctcaagtc catgaccgtt gaggcatcgg
     3181 gcaagctcgc acgaaaactg accgatcaac tcgtcaaccg ctggtcttac gagcttacaa
     3241 acaccacgac cataaaactg attgcgtcgg gactgaggtc ttaccgggat ctggtgaaat
     3301 tcaaggaatt gatcacgaac gaagtcaagg gagtgaagga ggtcaaccaa agagaatacc
     3361 gtaatgggca cgtggaaata gacctggagg tccggggaaa cgtacacggc gtagccgatg
     3421 acatagccgc cttgcgcatc aacggcaagg ccgtcagggt acagggtatc tcgcagaaca
     3481 cgataaaggt cagggtcggg ccgtgatgag gaaatttcga ctttcgagga ggatagaaat
     3541 gggaaggctc agcataagta agtgcttagc ggtttttttc tgtgtcgctt tcgttgtcgg
     3601 atgcgcgggg ggtgaaagct tcaagagggg ggaacagctt gctgctgaga ggcgatggga
     3661 cgaggcgata gagttttatc agaaggcgct tgaggagaac ccgagcagta aagaatacaa
     3721 agaatccctc gaaaaggcca ggcaggagtc cgcgaagatc catttcgaaa aagccaaaca
     3781 gttgatgccg gcggaaaagc aggcggacat aaccaggctc gaacaagtag tcaaggaggc
     3841 cgaccttgcc tacaagcttg acccgcagaa caaggccatc gcagcatttt atgaagacgt
     3901 caacagaaag aaaagcgagt tgcaatcact ggtcaagacg ctctacagcc aggccgatgc
     3961 ggacttaaaa aaagacgact ggctagcggc gatccagaaa ttgaatagaa tcaatgagat
     4021 tttccctggt tatgaagaca ctggcgacaa gcttgcccag gccgtccagc aggcttcagg
     4081 ggcgtactac aagcggggca tcgacctgga aaagcaggag gactggaggg gcgcaacgaa
     4141 ggctttcaag gccgtagtcg acataaaccc ggggtatctc gacgccgcga agcgctatga
     4201 agaagccagg agtagggaca acgtcggtta ctgtatcaag gcgggtaacg ccgccgccca
     4261 ggctatggat tggcagagag cggtcatgtt cttcgaaagg gcactggaat atgacccgtc
     4321 gaatcgagaa gtggccgggc aactggagaa actaaaggag aaagccgcca agagcttgtt
     4381 cgatcaggca atcaaagacg ttcagagggg gaaaatttac cagggcgcca agaaattgga
     4441 gcttgccaag acttatttgc cctctctcca ggaggacacc ctattcaagg agtttgtggc
     4501 gggaaacctc ggggtcaagc tcgtcgaccg ggccgagcag tatgcagaga aaggcaaatg
     4561 gggcaacgcc ctcatcgcct tacagcagct cgaggccctg gaccccaact acaaaggcct
     4621 gtttttcaag attcaggaag caaaagacaa catcaagaag aggatacgga aatccatcgc
     4681 gatcttcgat ttcaaaagcc ccagcagcca caaggatgcg ggaaggatcg tggccaacaa
     4741 gctcatgacc tttctttaca agaatgccag cggtgacatc aggatcatcg agcgggaaaa
     4801 cctggagtcg atcctcaagg aactccaact cgggcagacc ggccttgtcg acgtcgacac
     4861 tatgaaggcc ctcagtaaga tgggagggat cgacaccttc gtgatgggag acgttctccg
     4921 ttttacctcc gagtacaaag attttccgag catgaaccaa gtcaaagtcg tgatcgatga
     4981 ggaggaagtg aggaacccgg aatacgtgga gtggttgatc ttgcacaaga atccctcgga
     5041 agaggacttg aagacggctc ctccgaagac cgtgacgcgg aagaaccacc agttgatttc
     5101 ttacaagacc ggctacgcaa aaatcagcgc gtctatcgag gtctcctaca agttggttga
     5161 tgcaaggacg ggggagaatc tgttcacgaa cacagtcgcc gggaggttgt ccaaagagga
     5221 taagtaccag gatgccgtcc ctctggctgg catcaacggg gacccgctcg aattaccgac
     5281 ggagacggac gtcctggatg agctgactaa tgcgaaggta tcggaaatcg gccagagcgt
     5341 cctcaggcac ttctcgtccc tggaagtgga gtacaacaat caggccctcc tgcagatcaa
     5401 gaggaggaac cgagacgaag ccgtcgaacg cttcatggac gcgatcttcg acgagaacat
     5461 gaagggaata tcgacgccgg tgtcgcagag cgctaaagag atgatagaca agctgacgga
     5521 agatatgtag gaatgacata tgcattatgg gtttgcgcag tcttgggcat cgaaaaggcc
     5581 gtcgatgtgt cgggctggga aggtcatcat ggtcgcgatg tgtgtcgctt tagccgccgt
     5641 cctcgccttt ccgacgggat ctgtcgccca ggccaggact gtcaaactct ctgttttcaa
     5701 tttcggggcc tcgaacatag atgcttcttc gtacgccacg atggtgacca actacttgat
     5761 ttcgtcgttt cggggtaatc cagctttgag tgtgctcgac cgcaaggagc tcgaatattt
     5821 cctcttcctc aacgaccttc aacagaatga tgcgacggaa aacgtaatcc acatcggcag
     5881 tagacttgct ctcgattttg tcgtggcggg tacagtggga aagaccggtt cggcgatcac
     5941 ggtaaggtgc aaactctttt cggtcagtca gaagagggtc cttcagtcat ttctgataag
     6001 ggcctatggc gatgccgaac tgatgacgga gacgtacaaa ctgggagaac agatagcaaa
     6061 gttattgtcg aaggaagcgg agagccggaa ggacgagtcg gcgggactgc cgagtccgcc
     6121 ggtaaatgtc gaggcgagac cggggaacat gagcgtctac ctgaggtggg aagacccacc
     6181 gggggaaagc ccggcgggct acaaggtcta tcgtggcctt tccgagtcgg gaccttttgt
     6241 ccagattgcg cagcttcccc gacgtgagta cctggaccag ggccttgaaa agaagacaac
     6301 ctattactac cggttaaagt ccttcgacgg cagcggtatt cagagcagtg aatctccggt
     6361 agtcaaggtg gaaaccgtcc ccacgcccct tgcaccgata atattgaagg ctgagggcca
     6421 cgtcaaaagc gtgatcttga cctggtgcgt caacccccgc aagagcgacg accccttgcc
     6481 tttaaagggc ttcaaggtct atcgggcgga atccgccgac ggtccttaca ctgacacgca
     6541 caaagtgacc cttgaggaaa tcggaataac ctcgccagac aatgtctcgg cggaggtcaa
     6601 gattgcggtc aagtgccccg tcgacgggaa ggaatatttt ttcaagttga cggcctataa
     6661 cgaaaacaac cttgaaagca atttctccga gcctgtcagg gcatcttcga ttcccggagt
     6721 gcagaaaatt tcggcccagg gaggcttgat ccgtgaggtt aggctgagct gggtggctgt
     6781 agattccccg tttgtgaagg gttactggat atacaggagt gtgggagacg aggtggattt
     6841 tgtgagactg cggaaagtcg aggtttccta ttcgaccggt acgataaact actcggacgt
     6901 cgaagggctt gccgacgaga cgcggtataa ctatcgggtg accgcctttg aatatcctca
     6961 gacggagact gacccgtcgc ctgtggtgtc ggcagtgacc aaaggcaagc cgcccgtccc
     7021 ccgcgacgtc aaggcgataa gcgggcttgc ccggagagtt caggtcgtat ggacgcctaa
     7081 cacggaagac gacgtggagg ggtataagct ctaccggtcg aagacgaaag acggcaggtt
     7141 cgagtttgtc aagaaggtcc cggggaggaa ttcccagggc gttgtggacg aaggaggctt
     7201 gttcggcaag cttgaggatc aggcagagta tcattatatc ctcaggacct acaacaaggt
     7261 cgacgtggaa agcgatgatt ctgctgtggt ttcggccatg acaaaggcca agccgcagaa
     7321 gccgaccggt ttaaaatggg agacccaggg gaagaaagtc atccttacct ggtcgaagaa
     7381 cccagagccc gagatcgtaa cgtacagggt gtatgaaata atgtctagtg gcatcaagga
     7441 gataggtagg gtgaaggata ccagattctc cgtggacgcc cctggtagcg ggacgagcaa
     7501 gagatatgtc gtcactgctg tcgacaaaga cgacatcgaa agtgaacata gcgacgaagt
     7561 aacggtcgag aagtaaaaag atacgagacc tttgaaaaac gccccttcat gtcattccga
     7621 cggaagtcgg aatccagaac agattaaaaa cactggattc cgtgtcaagc acggaatgac
     7681 agtaaggcga tttaatcaaa aattcaaagg tctcgataca acaaaaaagg agatggggtg
     7741 cttaacgatg agaggtcatt tgttgaaagg aatggtcgtt gtgattgtcg gttttctcct
     7801 gtgtatggtt gttcccgacg ctgctatggc ccaggcatgc ggaaaattcc aggtcgttgc
     7861 gggagctgac gacaatgcct atctggtaaa cactgagact ggggccgtct gggtccttac
     7921 tcatcggacg ctggtttcgg gaacgggtag agaacctgtc gcgatacctt ataagtttct
     7981 gtatctgctc ccggagcaca acgcggtgct cgatgaaggg ggccagaagg ttcccgtcaa
     8041 agagagaaaa tcgcgttgac ctgatattcc tgcaggggac tgaggtatgg tgtgtcctgt
     8101 tccggcctgc ggtttttcga gaacctacgt gattacgggg gatacaggtc tcgccgtcgt
     8161 ggatgtggga agcgtcggtg ctgccgatga tgtgatggag ttcatcaaga ggagcctcgg
     8221 cggcgacgtg aaagacgtaa gagcgattct tgccacccac ttccatttcg accatatcgg
     8281 gggaataggc agactccttt tctccgccgc cgccgacaca aaggtgtttt ttcaccggct
     8341 ggtcaaagat tacctggagg ggaggaatgt cctcgcgccg atgaaaaact ggtgtaccgc
     8401 cttcggtcca acggcgtgga aaagcgcggg ctacgtccgg aaaccgagcc atctccactt
     8461 cgagagcctt gccggcattc ctttgccggc gctaagaaaa ctctcgacgc tgccctatcc
     8521 catggataga atcgtttttt ttgacggcga cggtgcaaga cggtacctgg cgggtttcga
     8581 cggctgggaa gtcatcgaaa ctccgggaca tacggaagat tccctgtcct tctaccacgg
     8641 aggttctgag gagttgatct gcggcgacct gatggtcaac atggataagg gcgggaaagg
     8701 gagactgaac ggcttctatg cgtcgaaaga aggtgttgtc gagtccttcg attttctgtt
     8761 gcggaacgtg aaggcaaaga cgatctatcc cggccacggt gagcccgtac agagtgaggg
     8821 gaacgcgctg cgcggcgtgg aggcgttcgc ggtcggccgg gatgacgcgt tgaaggacat
     8881 cggatgaatg ggcagcgagc agggataccc gggatgcttg gactttccgg gtcgtatttg
     8941 ctttatgatt ctttcgtcat tccgccgagg ggttgatgtg caatcgttga gccgattgac
     9001 tgcatttatt ctgtcgatag cctttatcgt gatgacgaca ggctgcgtgg tatacaccaa
     9061 aaaaaccaag atcacccctc agatgacatc gcttttcaag ggcacctaca aagtggaccc
     9121 ttacatggag gatcacaagc cgcggactgt cgccgtcctt ccctttatcg acaagtcggg
     9181 gagtgtagag ggttcccagg aggttcggaa gagtttctac aaccatttca gctccttacc
     9241 cttcaaagat atggagttgt accgtgtcga ccgcttgctc gcgaaggcgg gtctcacgga
     9301 tgcagccgag attaataaaa aaacccccca ggagctcggc aagatactgg aagtggacgc
     9361 cgtcgtctat ggggaaattt caaacttcga taagttcttt gcagggatat attctcaggt
     9421 ctccgttggc gccgaggtca ggatgtacga gacgaagacg ggtcattttc tctggagcgg
     9481 ccagcatgtg gcgcggattc acgaaggggg aatagcaacg actcccgtgg gcatcatcgc
     9541 aacggttatc gccgcggcca tgaacatgag ggacatccaa cttctcaggg cctgtgacga
     9601 cctcttccgg gacatggtaa agacaatacc cacgccgacg atcaccgagg ccatgaggcc
     9661 accggtcatc actctgctta cccaggacac gaaaggattg ccgaagaagg cgggcgatga
     9721 gattagggta gtcatccaag ggaccccgaa gatgcaggcg accttcgaca tcggggacta
     9781 caggaagaac atcgacatgc aggaggtaga accgggcggc tatctcggtg tttacagggt
     9841 cgttccgggg gacaacgtcg aaaacgccat cataacgggc catctgaccg acgactcggg
     9901 caacaggacg gactgggttg acgccatcgg gttcgtaacg ttggacacca caccgccgca
     9961 gaagccgaaa ggcctgagcg gcgtcgggcg tgacaagcac aacctcctga gatgggagaa
    10021 gaatacggaa ccggacctta ccggttaccg cctctacagg agccagacgc cgctcacggg
    10081 attcgtcgag gtggcgaaga cggagctcac ggaagcgaag gacgttgacc tcgtcaactc
    10141 ccagaaatac tattaccggg tgtcggcatt agaccgcgcg ggcaacgaaa gcgaaaagtc
    10201 ggaagcggtc tgcgtgatgc ccgtggcgcc tggtccgacg gccgtgtccg gggcgatcgt
    10261 agcagacacg acgtggtatt ccggggcaag cccgtatgtc atcgaggaca cggtgactgt
    10321 tgtcgacaag gccgtcctga cggtcgaacc ggggacggaa atccgttcaa agggtaaggg
    10381 gatcgtcgtc gaagggcgga tcatggcgct tggagaccgt gagagtctca tcaccttcag
    10441 cgggatggat gggacccggt gggagggtat atctttccgg aatgtgaggg agaaggagaa
    10501 tgtcattcga ttcgcccgcg tccgtaacgc cctgacgggt gtcacctgtg agtcctcttc
    10561 gccgcggata gaggactccg agttcatcga caacaccgag gccgtaaaaa taagcggggc
    10621 cttttcaaaa ccgtctcttt cgcgcaacac cctccagagg aacgagacgg gcgtgacgat
    10681 agcagagggc gcgcagccgt acctcgcaga gaacttaatc cgggacaacg caaagggcgg
    10741 gatagtggcg agcaatgccg ccccgtcgat tcagcgtaac acggtcatcc agaatcaggg
    10801 gtcgggtgtt gtcgtccgga gcagccaggc tgcggtcgcg gagaacaaca tcttcgacaa
    10861 cactcctttc gacatggtcg ggtcgtcgtc tggagaaccg gtgaaggcgc acaataactg
    10921 gtgggggacg gcgcagggtt ttgaggtatt caagaaggtc caggggcggg tcgacgtggc
    10981 caagattctg gacggccctt atcccggagg gaagccgctg gaacttccgg tgctctcgtc
    11041 gaacttggga gggcctctga ccggtgatgg gtatctcgtt ctctcgacca gcccgtaccg
    11101 ggtgacgaag gatgttatcg tcgacggggg acatgtcctg tacatccagt ccggcgtcca
    11161 ggtgctctac gaccagaaga cgtcgatcgt cgtgaaggat ggcggagtcg tggcgaaggg
    11221 cacaagggaa aggccgatcc ttttttccgc tgccgctgct tctccggttc ccggtttcta
    11281 cgcccatgcc ctacagttcg ctgctccgac gaaggtaaac agcttcctgg aatacgccat
    11341 agtcaagtat gccgacacgg ccatcgatgt tcagcacggt tcgcccgata tttcatacag
    11401 ctacatcgcg gaaaacgccc aggcaggtct tttctgcggc aacgacgcct caccgaaaat
    11461 caccttcaat acgttctacg gcaaccaggg tcaaggggcg atcgcctgcc gggggatgtc
    11521 gcgacccaag atcaaccata acaacttcgt cgggaacgcc tttgccatgc aggc
//
LOCUS       DAOTMX010000010        11396 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_5373_length_11396_cov_3.625430, whole genome shotgun sequence.
ACCESSION   DAOTMX010000010 DAOTMX010000000
VERSION     DAOTMX010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 11396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11396
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5373_length_11396_cov_3.625430"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="P5573_00610"
     CDS             <1..355
                     /locus_tag="P5573_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HRT26599.1"
                     /translation="ILLDFMLARFEAMMENLIVYPERMLANLNMTRGLIFSQMVLLKL
                     VEKGMSREDAYAVVQKNAMKTWMEGGDFKSLLLGDGEVMACLSSEELDRIFRVDNFLG
                     RLDTIFDRVFEGDDE"
     gene            complement(402..923)
                     /locus_tag="P5573_00615"
     CDS             complement(402..923)
                     /locus_tag="P5573_00615"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HRT26600.1"
                     /translation="MPLYEFNGKRPVVSADAFVHPEAVVIGDVSVGADSMIAPGAVVR
                     GDFGPITIGEGTSIQDGATVHVTPGDRVLIGNDIIVAHNASLHDVTIAQRCIVGIGAV
                     LLQGVVCEEGVVVAAGSVVSQGMRIPAGKLVAGNPARVIKDVPPEIAAYVEEGIEEYR
                     KLTRLYRLTMKRL"
     gene            complement(950..2578)
                     /gene="nadB"
                     /locus_tag="P5573_00620"
     CDS             complement(950..2578)
                     /gene="nadB"
                     /locus_tag="P5573_00620"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HRT26601.1"
                     /translation="MEIESDFLVIGSGIAGLSFAIKAAKLGTVAIITKKEEVESATNL
                     AQGGIAAVTDKTDSFESHIADTLECGAGLCKEDVVRFVVTEGPERIKELIEWGVEFTR
                     VEDASRSIFDLGREGGHSRRRVLHAKDRTGQEIERALIEKAASEKNIRVYENFIGIDL
                     IMASQVLGRRTGTDRCLGVYAYDIVHGEVHTFRAKFTLLATGGAGKVYLVTSNPDIAT
                     GDGIAMAYRAGALIANMEFIQFHPTCLYHPEAKSFLISEALRGEGGVLRLKNGTAFME
                     KYHPLKDLAPRDVVARAIDAELKKSGDEFVLLDITHRGREFIRRRFPNIYNECLKYGI
                     DMAKEPIPVVPAAHYLCGGAMVDAYGKTSIDRLFACGEVSCTGLHGANRLASNSLLEA
                     VVFAHRSFLKVLELLKEVKDDHLPIPPWDMRGVRESDESVVVTNDWDEIRRCMWNYVG
                     IVRSNKRLERAKRRIDLICEEIHEYYWNYTVTRDLLELRNIATVAKLIICSALMRKES
                     RGLHYNVDYPERDDVHWLRDTIISRYDGEKMKDA"
     gene            2866..4017
                     /locus_tag="P5573_00625"
     CDS             2866..4017
                     /locus_tag="P5573_00625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family lipid ABC transporter permease
                     subunit"
                     /protein_id="HRT26602.1"
                     /translation="MMKSENRKDYTLEIRGPKGGDVLLVITGRIVLGNLHQVIDDLRA
                     IPAESAPSSLTVDLSGFEYLDSSGALALLHLKHEAEARSIPFEFIGMNSRVKGIMDLL
                     HPVAIGAASTAPERKPPSLIEQVGEAGMKVAQDFVTLMSFTGALVTTLLYSIAHPRSV
                     RWDDVLRNMRQVGVDGFPIVTLISVLLGLIMAFMSSLQLKQFGANIYVASLVSIAMVK
                     ELGPIMTAILVAGRSGSAFAAEIGTMVVNDEVNALVTMGFDPLRFLVVPRIIAAAVVV
                     PLLTLYADAFAILGSLVVGVLGLDLTVYTYMQQIRITIDNYDVMTGFVKSLFFALLVG
                     GIGCQRGFQVRGGPESVGRATTSAVVSAIFLIILADSIFAIVLHYLERK"
     gene            4027..4785
                     /locus_tag="P5573_00630"
     CDS             4027..4785
                     /locus_tag="P5573_00630"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRT26603.1"
                     /translation="MTEQATPVIVVEGLTARYDERSILENVSFRVFQGERFIILGGSG
                     CGKSTLLKHLIGLYRPYSGHILVNGIDIASHDEENLGKVRMNIGVLFQNDALFGSMTL
                     SENVALPLQEYTDLPEGVIEQIVRMKLDLVRLGGYENHFPSELSGGMRKRAGIARALA
                     LDPQMLFLDEPWAGLDPITAAELDILVRSINTGIGATMVIVTQELASIFAVGQRVIML
                     DKEAKGIIAEGDPIVLREESADPRVVNFFNRRAA"
     gene            4802..5737
                     /locus_tag="P5573_00635"
     CDS             4802..5737
                     /locus_tag="P5573_00635"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HRT26604.1"
                     /translation="MARKVPRFLIGVFVIAGLLLTAVVIIWLGATRYFEGGKTYVTFF
                     DESVQGLQRDSTVKYRGVVIGRVESIEVAPDNVLIQVVMRIELGEDWENKATARLRPV
                     GITGIAFLDLEPMDPSKPDMSPKLSFVPKYPVIRSRPSDVRMLISNIEQVVNEMKELD
                     VGRMISRVEAAAVAGEKLMKRPEIDRVLKNLESITAHFDSVTARMDRAVGKGDIEVIL
                     SDAKDTTRELKGLATQLRASIEALRLPDVAGKMNRTIDNLDRKTATIAEEITAAGESL
                     QRASENMEMLMERLKTNPSDILFSRPPKGRGSGEQ"
     gene            5756..6391
                     /locus_tag="P5573_00640"
     CDS             5756..6391
                     /locus_tag="P5573_00640"
                     /inference="COORDINATES: protein motif:HMM:NF015823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HRT26605.1"
                     /translation="MVRIRPGVAFFILIVVFITGCVGLGRPSPQARMYAVEYPEPAFK
                     GLPPVNEAVRVGRLSSSRVFSGRAILYRPEPFSLAAYPYDYWAAAPADMVRDLLARDL
                     RVSGLFKAVFARQDYDNARFHLDGEIVEFFEDDTGGPAAVIRLDAVVFDRKGGKDASP
                     LLQRSYRFREPCERNARGVAQGMSRAVANLSERLILDVYHSASRVSTGSNR"
     gene            6445..7482
                     /gene="tsaD"
                     /locus_tag="P5573_00645"
     CDS             6445..7482
                     /gene="tsaD"
                     /locus_tag="P5573_00645"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HRT26606.1"
                     /translation="MLVLAIETSCDETAASVVLDGRRVLSNVIASQIDVHSRYGGVVP
                     EIASRKHMEAVVPVVMQALDDAGVELREIEGIAVTRGPGLVGSLLVGLQVAKAIAYAT
                     DRPFVGVNHLEGHVAAVFLGPQAPNFPFVALVVSGGHTNTYRVDGFDSFKTIGQTRDD
                     AAGEAFDKAAKLLGIGYPGGVVIDRLSRGADYERIRFPRSMKGSADFSFSGLKTSLLA
                     YLKKRERPVTEEEVPAIVASYQEAIVDVLVEKTLKAAEENGVKQVAVVGGVAANSRLR
                     QRFTADARERGIAVFIPDIKLCTDNAAMIAVVGDYRMSRGERDSFDLNALSRWPLDLK
                     AGVSRATAKDE"
     gene            7479..7982
                     /locus_tag="P5573_00650"
     CDS             7479..7982
                     /locus_tag="P5573_00650"
                     /inference="COORDINATES: protein motif:HMM:NF021363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HRT26607.1"
                     /translation="MIGLLKKKAREFYDRFISLKGNPRSIAMGMAIGVFVGVTPTIPF
                     HTVIILFLILISRQNLTAALLGAWITNPVTIPFFYFTEYELGRFLLGMGPCNVAVQEY
                     SIREIIAAGWSLFYPLQVGGLVLAPFFAVPAYFITHKMVLVLRRRNHDRDGESKRPTP
                     ETWNSPA"
     gene            7924..8781
                     /gene="rsmA"
                     /locus_tag="P5573_00655"
     CDS             7924..8781
                     /gene="rsmA"
                     /locus_tag="P5573_00655"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HRT26608.1"
                     /translation="MIETASPRDLLRRHGIRPLKRLGQSFLIDSNVIEKVVRTAGVKP
                     GDTVVEIGAGLGVMTAMVARKAGKVIALEVDRKVAAVLKEELRDVENVEAVEADVLLF
                     DFPSLSPRLKVIGNIPYNISTPILFRLLEFRNRIDSITVMLQKEVGDRIAASPGSKAY
                     GILSVVLSMYFEVSREFTVSADCFYPRPKVDSVVLRMVVRENPVFPLASDDFFRRLVK
                     TALGQRRKTLMNTLRRSPLFRPSSEGDILRILQDLGIEGRRRGETLTVEEFGRLSNAL
                     LPLTISLTD"
     gene            8920..9777
                     /gene="panC"
                     /locus_tag="P5573_00660"
     CDS             8920..9777
                     /gene="panC"
                     /locus_tag="P5573_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041584793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HRT26609.1"
                     /translation="MKIIESVREMQRHSEALRLQGKRVAFVPTMGFLHRGHLSLMEEA
                     RRRGDCVVTSIYVNPTQFGPTEDLDKYPRDFERDRQLCQEVGVDVIFYPSNNEMYPEH
                     YQTYVTVEGVTQNLCGLSRPGHFRGVATVCAKLFNMVKPHVAVFGKKDFQQLVVIRRM
                     VKDLNMDLDVVGMPTVREVDGLAMSSRNTYLKAEERESALSLSRSLVLAKKMYDAGER
                     SAARIIEEVKRFISSHPHAKIDYVQVCDTTTMKDVDSIVGECVIALAVRVGVARLIDN
                     YVFGEPLEI"
     gene            9816..10175
                     /locus_tag="P5573_00665"
     CDS             9816..10175
                     /locus_tag="P5573_00665"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HRT26610.1"
                     /translation="MHRFMLKSKIHRVTVTEANLDYEGSITIDEDLIEAADIIPYERV
                     DVYNISNGERFSTYVIKGERGSGTVCLNGAAARKAAKGDIVIIASYVPVADEVAKEWS
                     PKCVYVDGRNRQKGKGQ"
     gene            10325..10948
                     /locus_tag="P5573_00670"
     CDS             10325..10948
                     /locus_tag="P5573_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HRT26611.1"
                     /translation="MGKKVKNIFLAGLAVVIPVGVTVYILAFIVGMMDSFLAVLPGKV
                     HPDRMLGFHVPGLGVIFTALLVFLAGLITRSWLGYKVVGAGERLVTRIPVVKNIYQAT
                     KQLVDALFGGKVRDFRKVVLIEFPRRGVYTLAFVTGRTTGEAQKKTGEGRLNVFVPTT
                     PNPTSGYYLIVPEEEAVDLDMKVEDALKLLISGGLVVPERGRAVREE"
     gene            10993..>11396
                     /locus_tag="P5573_00675"
     CDS             10993..>11396
                     /locus_tag="P5573_00675"
                     /inference="COORDINATES: protein motif:HMM:NF012653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HRT26612.1"
                     /translation="MLITTESIKVGHPDTVADYIAARIIAAIIDEEKKNGMTIDTMPH
                     CGLECFVGKGFCLVGGEVTTNVWVDVEKIVRRTVLDLGYDDFAVGLDGASMGVLNAII
                     PQSPDINIGTRADLGKFKEIGAGDQGIMYGYA"
BASE COUNT         2433 a         3032 c         3489 g         2442 t
ORIGIN      
        1 gattctcctc gatttcatgc tggcgcggtt cgaggccatg atggaaaatc tcatcgtcta
       61 cccggaaagg atgctcgcga acctcaatat gactcggggg ctgatcttct cgcagatggt
      121 cctcctgaag ctggtggaaa agggcatgtc acgggaagac gcctacgctg tcgtacagaa
      181 aaacgcgatg aagacatgga tggaaggcgg cgacttcaag tcgcttctcc ttggagacgg
      241 tgaggtcatg gcctgcctca gcagtgagga attagaccgg atattccggg tggataattt
      301 tctcggccgt ctggacacga tattcgacag ggtattcgag ggggacgacg aatagttcag
      361 gaccgggagg ataatcccag gggtcaatag aggagggaag gctaaagtct tttcatcgtg
      421 aggcggtaga gtcgcgtaag cttgcggtac tcctcgattc cttcttccac gtatgccgct
      481 atctccggcg ggacgtcttt gatgaccctc gcggggttgc cagcaacgag ttttcccgcc
      541 ggtatacgca tcccctgtga aacaacggag ccggcagcga ccacgacccc ttcttcacag
      601 acgactccct ggaggagaac ggcgcctatc ccgacgatgc acctttgagc gatagtcacg
      661 tcatggagag aggcattgtg ggccacgatt atgtcgtttc ctatcagaac cctgtcgccg
      721 ggcgtcacgt ggaccgtcgc accgtcctgt atgctcgttc cttcaccgat tgtgatcggc
      781 ccgaagtcac ccctgacgac ggcacccggc gctatcatac tgtctgcgcc gacggaaacg
      841 tctccgatga cgacggcctc ggggtggacg aaggcgtcgg cggaaaccac aggcctcttt
      901 ccgttgaatt catagagcgg catggttgtt ccaacccaat ggagtgaatc tacgcatcct
      961 tcattttctc cccgtcgtac cgggaaatga tcgtgtccct cagccagtgg acgtcgtctc
     1021 tttccgggta atcaacgttg tagtggaggc cgcggctttc cttcctcata agggcgctgc
     1081 agatgatcag tttggccacg gtcgcgatgt tccgcagttc cagcaggtcg cgcgtgaccg
     1141 tgtagttcca gtagtattcg tggatttctt cacagatgag gtcaatgcgt cttttcgcgc
     1201 gctccagtct cttgtttgac ctgacgatgc cgacgtaatt ccacatacac cgccgtattt
     1261 cgtcccagtc gttggtgacg acgacggatt cgtcgctctc ccggactccc ctcatgtccc
     1321 agggaggtat aggaaggtgg tcgtccttca cttccttcaa taattccaag accttgagaa
     1381 atgagcggtg ggcgaagacg acggcctcga ggagggagtt gctagccagt cgattcgctc
     1441 cgtgcaggcc cgtgcaggag acctcgccgc aggcgaagag cctgtcgatg ctggtcttgc
     1501 cgtaggcatc caccatagcg cctccacaga ggtagtgcgc ggccgggacg acggggatcg
     1561 gctccttggc catatcgatg ccgtacttga gacattcatt gtagatgttg gggaagcggc
     1621 gcctgatgaa ttctctgccg cggtgggtga tgtcgagcaa gacgaactca tcgccggact
     1681 ttttcagttc cgcgtcgatc gcccgcgcca ccacgtcccg gggggcgaga tccttgaggg
     1741 gatgatactt ttccatgaaa gccgtgccgt tcttcaggcg caggacccct ccttcacccc
     1801 tgagggcctc gcttatgagg aaggacttcg cttccgggtg gtagaggcag gtgggatgga
     1861 actggatgaa ctccatgttg gcgatgaggg ccccggcacg gtacgccatg gcgatgccgt
     1921 cgcccgtcgc gatgtccggg ttggatgtga caagatagac cttaccggcc ccgccggtgg
     1981 cgaggagggt gaactttgcc cggaaggtgt ggacctcgcc gtgaacgatg tcgtaggcgt
     2041 aaacccccag gcatctgtcg gttcccgtcc tccggccaag gacctgggag gccatgatca
     2101 ggtcgatgcc gataaaattc tcatagaccc gtatgttctt ctcggaagcg gctttttcga
     2161 tgagggcccg ttcgatctcc tgacccgtgc ggtccttggc gtgcaggacg cgccggcggg
     2221 aatggccgcc ttccctgcca aggtcgaaga tactcctgct tgcgtcttcc accctagtaa
     2281 actcgacccc ccattcgatc aattccttga ttctctccgg gccttcggtc acaacgaaac
     2341 ggaccacatc ttctttgcag agtccggcgc cgcattcgag cgtatccgcg atgtgcgatt
     2401 cgaaactgtc ggtcttgtcg gtgaccgccg cgataccacc ctgggccagg ttggtggccg
     2461 attccacctc ttctttcttc gtgatgatgg cgaccgtccc cagcttggct gctttaatgg
     2521 cgaagctcag ccctgcgatc ccactgccga tgacgagaaa atccgattca atttccattt
     2581 gttatgacct atcagcccga acttcgaacg gccttgagtc cgactgaatc aaggacgaga
     2641 aatttctaac agaaagttcg taggttaaca agccctttcc gtgcctcgcc gtagtcctac
     2701 ttcgcgggcg tctgtaatgc ctgatgcccc gtccgtcttc ctcttggcaa ggtgaaaccg
     2761 tgcaaaaagg aaggcggcga taaatccggg aagcgattgt ggttttggtt tgctttttgc
     2821 gctgcattga ttgataattc atcatgtttt ccccggtact tccgaatgat gaaaagtgaa
     2881 aatagaaaag attacaccct tgaaatccgg gggccgaagg ggggggatgt tctccttgtc
     2941 atcaccggca ggatcgtcct cggtaatctt caccaggtga tcgacgattt acgggccatt
     3001 ccggcggaat cggccccttc gagcttgacg gtcgacctgt ccggcttcga atacctggac
     3061 agttccggtg cccttgccct tctgcatctc aaacacgagg ctgaggcccg gtccatcccc
     3121 ttcgaattta tcgggatgaa cagccgggtg aagggcataa tggaccttct ccatcccgta
     3181 gccatcgggg ccgcgtcaac cgcgccggaa aggaagcctc cgagcctcat cgaacaggtc
     3241 ggcgaggccg gaatgaaggt cgcgcaggat tttgtgaccc tcatgtcctt cacgggagcc
     3301 ctggtgacga ccctcctgta ctcgattgct cacccccggt ccgtacggtg ggacgatgtc
     3361 ttgcgcaaca tgaggcaggt gggtgtcgac ggttttccga tcgtcacgct cataagcgtg
     3421 ctcctcggcc tcatcatggc gtttatgtct tccttacagc tgaaacagtt cggggcgaac
     3481 atctacgtgg cctcgctcgt ttcgatagcc atggtcaagg aactggggcc gatcatgacg
     3541 gccattctcg tggcggggcg gtcggggtcg gccttcgccg ccgagatcgg cacgatggtg
     3601 gtcaacgacg aggtcaatgc cctggtgacc atgggattcg accctcttcg gtttctagtc
     3661 gtgccccgga tcatcgccgc cgccgtcgtg gttcctctat tgaccctcta tgccgacgcg
     3721 ttcgccatcc tcggcagctt ggtcgtcggc gtcctgggcc ttgatttgac tgtatacacg
     3781 tacatgcaac agataaggat cacgatagac aactacgatg tgatgacggg gtttgtgaag
     3841 tccctgttct ttgcgttact cgtcggaggg atcggttgtc agaggggatt tcaagtcagg
     3901 ggcggcccag aatctgtcgg cagggccacg acatccgccg tggtttctgc catcttcctg
     3961 atcatcctgg ccgattcgat ttttgcgata gtactccatt atctcgaaag aaagtgaggc
     4021 gccgtcatga ccgaacaggc aacgccggtg atcgtcgtcg agggattgac cgcccgttat
     4081 gacgagagaa gcatcctgga aaacgtgagt ttccgggtct ttcaggggga gaggtttatc
     4141 atcctcggtg ggagcggttg cggcaaatcg acgctcctca aacacctcat cggcctctac
     4201 cggccttact ccgggcatat attggtcaac ggcatagaca tcgcgtccca cgatgaagag
     4261 aatttaggaa aagtgaggat gaacatcggg gtacttttcc agaacgatgc ccttttcggc
     4321 tccatgaccc tttccgagaa cgtggccctt cctctgcagg aatacaccga cctgccggag
     4381 ggcgtcatcg agcagatcgt ccggatgaag ttggatctcg tccgtctcgg cgggtacgag
     4441 aatcacttcc cctcggagtt gtcggggggg atgcggaaga gggcggggat cgcgagagcg
     4501 ctggccctcg acccccagat gctttttctc gacgagccct gggccgggtt ggatcccatc
     4561 acggccgcgg aattagacat cctggtcagg agcatcaaca cggggatcgg cgccaccatg
     4621 gtgatcgtca cgcaggagct agcgtcgata ttcgctgtcg gccagcgggt gataatgttg
     4681 gacaaggaag ccaagggcat catcgcggag ggggacccca tcgtgctgcg tgaggaatcc
     4741 gcagaccccc gggtcgtcaa tttcttcaac aggcgggcgg cgtagaaaga gagaagcgga
     4801 catggcaagg aaagtgccgc ggtttttgat cggggttttt gtgatagccg gtttgctcct
     4861 gacggcggtc gtcatcatat ggctcggcgc gacgaggtac ttcgaaggcg gaaagaccta
     4921 tgtgaccttt tttgacgaat ccgtgcaggg gcttcagcgg gattcgaccg taaagtaccg
     4981 gggggtagtc atcggaagag tggagtcgat cgaagtggcc ccggacaatg tattgatcca
     5041 ggtagtgatg aggatagagc tcggagaaga ctgggagaat aaggctacgg cccgcttgcg
     5101 accggtgggg attaccggta tagcgttcct cgacctcgag ccgatggatc cgagtaaacc
     5161 ggatatgtcc ccgaaactct ctttcgtccc caaataccct gtgataaggt cccggccttc
     5221 ggatgtgcgt atgttaatct cgaacatcga gcaagtagtc aatgagatga aagaactgga
     5281 cgtggggagg atgatctccc gggtggaggc ggccgcggtt gccggcgaga aacttatgaa
     5341 aaggccggaa atcgaccgag ttctgaagaa cctggagtcc atcaccgcgc acttcgacag
     5401 cgtcaccgca aggatggaca gggccgtggg aaagggggac atcgaggtca tcctttccga
     5461 tgcaaaggat accacccgag aattgaaggg actcgcaacg cagttacggg cgagcatcga
     5521 agccctccgt ttgccggatg tggcggggaa gatgaaccgc acgatagaca accttgaccg
     5581 aaagacggcg acgatcgccg aggagataac agcggccggc gagagcctcc agcgggcgtc
     5641 ggaaaacatg gagatgctga tggagcgctt gaagaccaac ccatcggaca tactcttcag
     5701 caggccgccg aagggtagag gcagcggtga acaataaaaa aggtgagtgg taaacatggt
     5761 taggattagg cccggagtcg ctttctttat tctgatcgtt gtctttatca ccgggtgtgt
     5821 cggcctggga aggccgtcgc cccaggcgag gatgtacgcg gttgaatacc cggaacccgc
     5881 cttcaaagga cttcctccgg tgaatgaggc agtgagggtg ggaaggctgt cgtcgtcgcg
     5941 ggttttcagc ggccgggcca tactttaccg gccggaacct ttctcgcttg ccgcttatcc
     6001 ctatgattac tgggccgcgg ccccggcgga tatggtcagg gacttgttgg cccgcgacct
     6061 aagggtatcc gggcttttca aggcggtatt cgcccggcag gactacgata acgcccgttt
     6121 ccatctcgac ggggaaatcg tggaattctt cgaggacgac acgggcggcc cggcggcggt
     6181 catccggctg gatgcggtgg tttttgaccg gaagggcggc aaagacgcga gccccctttt
     6241 gcaaaggagt taccggttcc gtgaaccttg tgaacggaac gcccgggggg ttgcccaggg
     6301 gatgagcagg gcggtggcga acctgtcaga aaggcttatc ctggacgtct atcattccgc
     6361 ctcccgggtg tcgaccggtt cgaaccgctg acccttcggc tcgaccccct tttttgggaa
     6421 ggaaaaacat gttaagggag acccatgctg gtcttggcca tagagacatc ctgtgacgaa
     6481 acggcggcga gcgtagttct cgacggcaga agggtgcttt cgaacgtgat cgcctcgcag
     6541 atagacgtcc acagccgata cggcggtgtg gttccggaga tcgcctcccg taagcacatg
     6601 gaggccgtcg tacccgtcgt catgcaggcc ctggatgatg ccggagtcga gctgcgggaa
     6661 atagaaggga tagccgtgac ccgcgggccg ggactcgtgg ggtctcttct ggtggggctt
     6721 caggtggcaa aggccattgc ctatgccacg gacagaccct ttgtcggcgt gaaccatctg
     6781 gaaggccatg tagcggcggt attcctcggc ccgcaggccc cgaactttcc tttcgtcgcc
     6841 ctggtggttt cgggcggcca tacgaacact tacagggtag acggtttcga cagcttcaag
     6901 accatcgggc agacgaggga tgatgcggct ggcgaggcct tcgacaaggc ggccaagctt
     6961 ctgggtatcg gctatcccgg aggagtcgtc atcgacaggc tgtcgcgggg tgccgattat
     7021 gaacggatcc ggtttcccag gtccatgaag ggcagcgctg acttcagctt cagcggtttg
     7081 aagacctcgt tgcttgctta cctgaagaag agggagcggc ctgttaccga agaggaagtc
     7141 cccgctatcg tggcgagcta tcaggaggcg atagtcgatg tcctcgtcga gaagacgttg
     7201 aaagcggccg aggaaaacgg ggtgaaacag gtggccgtcg ttggcggcgt ggccgcaaac
     7261 agccgcctcc ggcaacggtt tacggccgac gcgagggagc gggggatcgc ggtcttcatc
     7321 cccgacatca aactgtgtac ggacaacgcc gccatgatcg cggtagtggg agactatcgg
     7381 atgagcaggg gggaaagaga cagtttcgac ctgaatgccc tgtcgagatg gcccctggac
     7441 ctcaaagccg gagtctcccg ggcgacagcc aaggacgaat gatcggacta ctcaaaaaaa
     7501 aggcccggga attctatgac cgattcatca gcctgaaggg gaacccccgc agtattgcga
     7561 tgggcatggc cataggtgtc ttcgtggggg tgacgccgac aatccccttc cataccgtca
     7621 tcatcctgtt tctcatcttg atttcccgcc agaacctgac ggcggcgctc ttaggggcat
     7681 ggatcaccaa cccggtgacc atcccctttt tttattttac agaatatgaa ctggggaggt
     7741 tcctgctggg catgggaccg tgcaacgtcg ccgtgcagga gtacagcata agagagatca
     7801 tcgcggccgg atggtcgtta ttctacccgt tacaggtcgg cggactcgtc ctggcccctt
     7861 tctttgcagt tcctgcctat ttcatcaccc acaaaatggt cctggttttg cgaaggcgta
     7921 atcatgatag agacggcgag tccaagagac ctactccgga gacatggaat tcgcccgctt
     7981 aagaggctgg gccagtcttt cctcatcgac tccaacgtta tcgagaaggt cgtgaggacg
     8041 gcaggcgtca agcccggcga caccgtcgtg gaaataggcg ccggacttgg cgtcatgacc
     8101 gcgatggtag cccgcaaggc agggaaggtt atcgccctgg aggtcgacag gaaagttgcg
     8161 gccgttctca aggaagagct gagggatgtc gagaacgtcg aggccgttga agccgatgtc
     8221 ctcctgttcg attttccgtc gctgtcccca aggctgaagg taatcggtaa cattccttac
     8281 aacatatcca caccgatcct cttccgcctc ctggagttca gaaaccgcat cgactcgata
     8341 acggtcatgc tgcaaaaaga agtcggcgac aggatcgccg cctcgccggg gagcaaggcc
     8401 tacgggatcc tgtcggtagt cctctctatg tacttcgagg tatcgcggga attcacagtt
     8461 tcggcggact gcttctaccc gaggccgaag gtcgattccg tcgtcctcag gatggtcgtc
     8521 cgggaaaatc ccgtcttccc cctggcgagc gacgacttct tccggcgtct tgtcaagacc
     8581 gctttgggac aaagaagaaa aacgctgatg aacaccttga gacgctcgcc cctctttcgt
     8641 ccctcttcgg agggggacat actccgcatt ctgcaagacc taggtatcga ggggcgcagg
     8701 aggggagaaa ccctgacggt cgaggaattc ggacggctga gcaacgccct tctgccgctc
     8761 acaatttcct tgacagactg agtccctttt ggtagggaaa atcgaccgcc tggatcccca
     8821 ggggactggg acggagggag agatagagcc aatcatccat ctaagattgg gtgccttaag
     8881 cctgccgtct caagcggcag gcttttttgt ggaggaagca tgaagataat cgaatccgtg
     8941 cgggagatgc aacggcattc ggaggcacta agacttcagg gaaagcgggt cgctttcgtc
     9001 cccaccatgg gctttctgca cagagggcac ctcagcctga tggaagaggc aagaagacgc
     9061 ggcgactgcg tcgtcaccag catctatgtg aatcccaccc aattcggccc cacggaagac
     9121 ttagacaagt atccccgtga cttcgaaaga gacaggcaac tctgtcagga agtgggcgtc
     9181 gacgtcatct tctatccttc caacaacgag atgtaccccg agcattatca gacttacgtg
     9241 acggtcgaag gggtgacgca gaacctctgc ggcctttccc ggcccggtca cttccgaggg
     9301 gtggcgacgg tctgcgcaaa actgttcaac atggtcaagc cccatgtcgc cgtcttcggg
     9361 aagaaggact ttcagcaact cgtggtgatc cggaggatgg tgaaggacct aaacatggac
     9421 ctcgacgtcg taggtatgcc caccgtgcgg gaagtagacg gcctcgctat gagttcgcgg
     9481 aatacgtatt tgaaggccga ggagcgggaa tcggcgctca gcctcagccg ttcgctcgta
     9541 ctcgcgaaga agatgtacga tgccggggag aggagtgcgg caaggatcat cgaggaagtg
     9601 aagagattca tatcgagcca cccccacgcg aagatcgact atgtccaggt ctgcgacacc
     9661 acgaccatga aggatgtcga cagtatagtg ggagagtgcg ttatagctct tgctgttagg
     9721 gtaggcgtcg cgcggctgat agataattac gtgttcggag aacctctgga gatatgagga
     9781 gaaagcatca gtaatagaat aaggtagggg gtcagatgca ccggtttatg cttaaatcga
     9841 agattcaccg ggtgacggtc accgaggcaa acctcgatta cgagggtagc ataacgatcg
     9901 atgaggacct gatcgaggcc gctgatatca ttccctatga acgggtggat gtttataaca
     9961 tatcgaacgg agaacgtttt tccacttatg tcatcaaagg ggaacggggt tccggcaccg
    10021 tctgtctcaa cggtgccgcc gcgcggaagg cggcgaaggg cgatatcgtg atcatcgcga
    10081 gctatgtgcc ggtggcggat gaagttgcga aagaatggtc gcccaagtgc gtctatgtgg
    10141 acggcagaaa caggcagaag gggaagggtc aatagggctg agggtaagaa aaaggtaatg
    10201 actgaggcga ggcgaataca gtacagcttc gcctttttat taaataaacg ttgaaacccg
    10261 tgggcttttt tgttaaagct acagccactt tttccggaaa gacagctctc tgataagaag
    10321 tcaaatgggc aagaaggtca aaaacatatt tctcgccggt cttgccgtag tcattcccgt
    10381 cggtgtgacg gtctacatcc tcgccttcat cgtcggcatg atggatagct tcctcgcggt
    10441 gctgcccggg aaggtccatc cggaccggat gcttggtttc catgtgccgg gcctgggagt
    10501 cattttcacc gctttactcg tttttttggc cggcctgatc acaagaagct ggttgggcta
    10561 caaggtcgtc ggcgcggggg agaggcttgt cacgaggatt cccgtcgtca agaacatcta
    10621 tcaggccacc aagcagcttg tcgacgccct tttcggcgga aaggtcaggg atttcaggaa
    10681 ggtggttctt attgagttcc cccgcagagg ggtgtataca ttggcgttcg tcacggggcg
    10741 gacgaccgga gaggcgcaga aaaagacggg cgaggggcgt ctgaacgtat ttgtgccgac
    10801 gacgcccaac ccgacgtcag gctactatct gatagtcccc gaggaagaag cagtggacct
    10861 agacatgaag gtcgaagatg ccttgaagct gctgatatcc ggggggttgg tcgtccctga
    10921 acgaggaagg gctgtcaggg aggagtagga taacagcgat agaatgattg atgaagcagg
    10981 agggaccgag acatgttgat cacgactgag tcgatcaagg ttgggcatcc cgatacggtc
    11041 gccgattaca tagcagcgag gatcatcgcc gccatcatcg acgaagagaa gaaaaacggg
    11101 atgacgatag acactatgcc ccattgcggg ctggagtgtt ttgtgggtaa gggtttttgc
    11161 cttgtcgggg gggaagtcac gacaaatgta tgggtagacg tggaaaagat cgtccgtcgg
    11221 accgtgcttg acctcggcta cgacgacttc gccgtcggcc tcgacggggc gtctatgggg
    11281 gtgttgaacg ccatcatccc ccaatccccg gacatcaata tcggcaccag ggcggacctg
    11341 gggaaattca aggagatcgg tgcgggagac caggggatca tgtacgggta cgcctg
//
LOCUS       DAOTMX010000011        10992 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_5608_length_10992_cov_4.313980, whole genome shotgun sequence.
ACCESSION   DAOTMX010000011 DAOTMX010000000
VERSION     DAOTMX010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 10992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10992
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5608_length_10992_cov_4.313980"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="P5573_00680"
     CDS             complement(<1..585)
                     /locus_tag="P5573_00680"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HRT26613.1"
                     /translation="MTVEDKEHRSFIGKALGIGGNEAVRITLLFRGGSDRTFYRVHVP
                     GRNSFIFMHYSPGREENNYYVEIAGFLPNIGVRVPAIVGHDRARRFILMEDLGDADLF
                     SFRQAPWSRREELYRKTLGLVLRLHSFPLDAFPFQAVPLAEAFGPSLYRWERDYFREN
                     FVGGLCGVRLDPGEAEDLERELAALAADLEGGSRC"
     gene            complement(607..1347)
                     /locus_tag="P5573_00685"
     CDS             complement(607..1347)
                     /locus_tag="P5573_00685"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRT26614.1"
                     /translation="MDGCAGKVRPIKTAFILGAGHGTRLRPLTEACPKPLLTVGGRPL
                     ITYAMEHLRSAGVERFIVNTHHCAEAYEKAFPERTWCGLPVIFRYEPVLLDTAGGIKN
                     IEDLLEEDETFFVYNGDVISDLPLRTLIAFHFSRGAEATLALRSEGPLLNVSLDGEGR
                     VCDMRDMLGNPGARRCQFTGIYILERRFLGRLEPGRVESVVPAFVRLISERPGSVAGV
                     VIDEGFWSDVGSVEEYRRVSGLSLGMWG"
     gene            complement(1349..2632)
                     /locus_tag="P5573_00690"
     CDS             complement(1349..2632)
                     /locus_tag="P5573_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmpG family muropeptide MFS transporter"
                     /protein_id="HRT26615.1"
                     /translation="MKHPKPSYRNPWTWVPSLYLAEGIPYAVVMTVSVVLYKRLGISN
                     TDIALYTSWLYLPWVIKPFWSPLVDMFRTKRFWILAMQVLLGASLAAVALTIPVPDFF
                     RWTLAVFWLMAFSSATHDIAADGFYMLGLSQREQAAFIGVRNMFYRIAMISAQGLLVI
                     LAGLLERRCPSVAAAWSATFFVLAGFFLASFLYHFFVLPYPASDRPAPRGLSKNFFAD
                     FLNSFALFFGKRDIAAVIAFLLFYRFAEAQLMKMVVPFLLDPRAAGGLGLSTAEVGIV
                     YGTVGVVMLMLGGLIGGYVVSRQGLKSWLWTMVAVMHLPDLVFVYLSWTQPENFFAVA
                     VAVAVEQFGYGFGFTAYALFMILASEGEYKTIHYAVCTGFMALGMMIPGMFSGWVQEM
                     LGYRNFFLWIMVSTIPGFIVPALVDIEPGFGKRGD"
     gene            complement(2639..3964)
                     /locus_tag="P5573_00695"
     CDS             complement(2639..3964)
                     /locus_tag="P5573_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HRT26616.1"
                     /translation="MINGEPTIRVGILDRCRVMRGRIDGSCLVAGRPVSGFFKSQPVD
                     GLVALFDSSGREIAKGREIRCTPKAGATFTLFDVAVGSRFHWEHKEEQTFEGNLILLA
                     GEGGAVTAANEVGLEAYLSSVVPSEMNPEGPPEFLKAQAITSRSWLAAMLERAARKEV
                     PPASGEIDGEGETVRWYDREAHDLFDVCADDHCQRYRGVAKAIPARAAAAVEETRGLF
                     LVHEGEVCDARFHKACGGLTEKYENVWEGRAVPYLSSVSDARHPFSPVVSEDDAERWI
                     LSNPDAYCNVTDEDVLRRVLPSSDLETGRFFRWEVTYTREELEAILQKKSGIDFGILR
                     ALIPLERGPSGRIVRLRIEGSKRSWVVGKELEIRRWLSESHLLSSAFVIGLERDGAGT
                     PAKFILRGAGWGHGVGLCQVGAAVMALKGHKAEEILRHYFRGAELKKLY"
     gene            complement(3964..4542)
                     /locus_tag="P5573_00700"
     CDS             complement(3964..4542)
                     /locus_tag="P5573_00700"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HRT26617.1"
                     /translation="MAKSLKGTKTEKNLLAAFAGESQARNRYTYFASAARKEGYEQIA
                     AFFLETAENEKEHAEVFFKYLEGGDVAISASYPAGVIADTKSNLEAAAAGENLEWTTL
                     YADFAAIAREEGFTEVAISFEQVAKVEKFHEQRYRKLAENVANNEVFAKKSPVKWHCR
                     NCGYVAEGTSAPKICPACRHPQSYYEVLAENY"
     gene            4815..5465
                     /locus_tag="P5573_00705"
     CDS             4815..5465
                     /locus_tag="P5573_00705"
                     /inference="COORDINATES: protein motif:HMM:NF016163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HRT26618.1"
                     /translation="MAKRKPSIVVGVDLAGSPSRPTGVCILRGLKAQTSVVFGDEEII
                     ELIKNTGPDLVTVDAPLSLPEGRTTIHDRSGGHFRECDRELRRRGIRFFPVTIGPMRM
                     LTERGMALKTKVERMDCPVMECYPGAAQDVWGIPRQHRDRKGLREGLKDLGVKGLAEA
                     LTGDELDAVTAALVGRWLLLGKGEIIGGSDGIVMPAREKTEARRGRRSRGQMCNHA"
     gene            5805..7814
                     /locus_tag="P5573_00710"
     CDS             5805..7814
                     /locus_tag="P5573_00710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRT26619.1"
                     /translation="MKDGCKTRQQLLDELSKLEQRILSLERVNKKLKKDKTGLEQDSK
                     RLRLIFDNAPVGVYMSTPEGRYLKVNRAQVDILGYESPEEMVRSVTDIKNQIYVEPED
                     RDRLEKLLAEQGIVKGFETRYYRKDGEIIWVSIYIQAAKDEKGKVFYQGTVVDITERK
                     QAEEALRESERRYRHLVNFVPIPLFEIDLAGNFKTGNLAISEAFGYTEKDVEKGLNIA
                     QVIIPEDIERIVSDMKEVLRKGKIRGSGPGYEYTGIRKDGSTFPFIAYASPIFHGDDP
                     VGFRGAIIDLTAQRQAEEALRKSISSLAEAQRIAHIGNWEWDVGSDDVYWSDELCRIV
                     GTSPQAFSGNFAAFCELFPSEDRETVKVAVRKAVKEGRKSDIDHRITRPDGTVRDLHQ
                     LVEPIIDRDGKTIRVIGLVQDVTEKRQRERELQNAKEQLIQAEKLSSLGRLSAGVAHE
                     VLNPATIISMELQLLQKMGDLPPHVQEELNICMEQISRIVTIAENLKQFSRIPVKHTA
                     MTDVNNLIEHELNLYAMQLKIEGIETDVQYDPDLPSIPIDREKIEQVIMNLITNAVSA
                     MEGKEKKVLRIKTGRNTVHRKGVCLTITVADTGHGISDEHMPKIFDPFFTTKEPGKGT
                     GLGLPISYGIVQEHGGMIWAENNEWGGATFYVSLPIQRDSLRNNA"
     gene            7831..8199
                     /locus_tag="P5573_00715"
     CDS             7831..8199
                     /locus_tag="P5573_00715"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRT26620.1"
                     /translation="MERILVVDDEAQIVRLLQKYFASKGYEVSTAGSGLEAIEKVKDV
                     RPHVVLLDILMPDMGGIDTLKEIKKIDPQIGVIMITAVIDEEIARRALELGADDYITK
                     PIDFDYLETCVMVKLAQLPG"
     gene            complement(8260..9702)
                     /locus_tag="P5573_00720"
     CDS             complement(8260..9702)
                     /locus_tag="P5573_00720"
                     /inference="COORDINATES: protein motif:HMM:NF023222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HRT26621.1"
                     /translation="MAIKTKAEYVEDLKGVKPDIYMLGEKVDRVWDDPRFQSTLNLIG
                     ATHDFSFDEEFKHLSVVHSPLVDEPVRRLNMHIQTSREDAITKVRLTREVTQRRICTW
                     CQSNELCVVWAATYDTDQKHGTCYHERFKEYVKYLMKNDYDIGWAMMDPKGDRSLMPS
                     QQKTPTDLRVVKKNSRGIVIRGAKVHTSYGPCMRELVVVPCRQLKEADKDYAVCCSMP
                     VDTKGVTFVARPAPNRSRTDAQMECPIGSAIGAVEAMTVFDDVFVPWERVFLCGEWDM
                     TGRAPHYFASIQRHSKCACLSGHTDLICGIVALIADVNGLGMKTAHIRDKATKLMMEA
                     ETAYGCAVGAAVEGEVHPSGVWIPNALIGNAGLHYIKNLSGEHIALVHDIAGGVIVTM
                     PTEQDYKNPTIKAWMDTYLAGNPKYTTEERLRVLYLAQEVAASRWTGYFVGWAINASG
                     STMTTEIVVRSAYDLEKRIEIAKRWAGVGE"
     gene            complement(9738..10142)
                     /locus_tag="P5573_00725"
     CDS             complement(9738..10142)
                     /locus_tag="P5573_00725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HRT26622.1"
                     /translation="MEKQRIRVLMVRLGMDAHWRGSMVVARALRDAGMEVIYGGNQMP
                     ANIVRTALQEGVDVIGLSSLSGNHMILAPEVVNLLKKEKAGDIKVVLGGTVPPGDVEK
                     LKKAGIAEVFGPGSSLKTIISRIESLKAVKAA"
     gene            complement(10168..>10992)
                     /locus_tag="P5573_00730"
     CDS             complement(10168..>10992)
                     /locus_tag="P5573_00730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRT26623.1"
                     /translation="SFFLSAHNDFFEEIAKYRAARRVYYNIMRQRFKAEDPRSLMFRF
                     HVQTAGVALTAQQPLNNIARSAYHALAAVLGGAQSVHVDAYDEALCTPTELSSVTALR
                     TQQILQNETGVINTIDPLGGSYYLESLTDRMEAEVLKILDEIEGKGGLVSSVESGWIH
                     AEISDAAYKYQCDIESGEMPIVGVNCFQIEDEVLPIELFSSPETLKVQKEKLRRIREK
                     RDPRKVQKSLDAVARCCERNENLMELIVGEVKNLITEGEISRTLKRVYGVWEPPLF"
BASE COUNT         2487 a         3232 c         3093 g         2180 t
ORIGIN      
        1 gcagcggctc ccaccctcga ggtccgctgc gagcgccgcc agctcccgct ccaggtcctc
       61 cgcctcgccg gggtccagtc tcacgccgca gaggcccccc acaaagtttt cccggaagta
      121 atcccgctcc caccggtaga gcgaagggcc gaaggcctcc gccaggggaa cggcttgaaa
      181 gggaaaggca tccaagggaa aagagtggag ccgaagaacg agacccaggg tcttccggta
      241 cagttcttcc cggcggctcc atggtgcctg ccggaaagaa aagaggtccg cgtcgccgag
      301 gtcctccatc aggataaagc ggcgggcgcg gtcatgcccg acgatcgcgg gaaccctaac
      361 gccgatattc gggagaaacc cggcaatttc tacgtagtag ttgttttctt cccgtcccgg
      421 gctgtaatgc atgaaaataa aggaatttcg ccccggcacg tggaccctgt aaaacgtccg
      481 gtccgaccct ccccgaaaga gcagggtgat gcggaccgcc tcgttccccc cgattccgag
      541 cgcttttcct atgaacgacc tatgttcctt atcttcaacc gtcatcgtat gccgacccgc
      601 ctgcctttaa ccccacattc caaggctcaa acccgaaacc cgcctgtatt cttccacgct
      661 cccgacgtcg ctccagaatc cttcatcgat cacgacgccc gccaccgacc ccggcctttc
      721 gctgatcagt cggacgaagg cggggacgac tgactcgaca cggcccggct ccaggcggcc
      781 caggaatcgc ctctcgagga tgtaaatacc ggtaaactgg caccgtctgg cgccggggtt
      841 gccaagcatg tcgcgcatat cgcagacccg cccctctccg tcaagagaaa cgttgaggag
      901 tggtccctcg ctccgtaggg caagcgttgc ctcggcgccc cgagagaagt gaaacgcgat
      961 gagtgttcga agcggtaggt cggatatgac gtctccgttg tagacaaaga aggtctcgtc
     1021 ttcctccaag aggtcctcga tgttcttgat gccgccggcg gtgtcgagca acaccggttc
     1081 atagcggaag atcacgggca ggccgcacca agttcgttcg ggaaaggcct tctcataggc
     1141 ctccgcacag tggtgcgtgt tgacgataaa gcgctcgacg cccgccgacc gcagatgctc
     1201 catcgcgtag gttatcaggg gacggccgcc gactgtcaaa aggggcttgg ggcaggcctc
     1261 cgtcaacggg cgaaggcggg tgccgtggcc ggcgccgagg atgaaagccg ttttgatggg
     1321 cctgactttt ccggcgcacc cgtccatcct aatcccccct cttaccgaac ccgggttcga
     1381 tgtcgacgag ggcgggcacg atgaaccccg ggatcgtgga gaccatgatc cagaggaaaa
     1441 agtttcggta accgagcatc tcctgtaccc acccgctgaa catccccggg atcatcatcc
     1501 cgagggccat gaaaccggtg cagacggcgt agtggatagt cttatactcc ccctcggagg
     1561 cgaggatcat gaaaagcgcg taggcggtga aaccgaagcc gtagccgaat tgttcgacgg
     1621 cgacggcgac ggcgacggca aaaaaattct ccggctgagt ccaggagagg tagacgaaga
     1681 caaggtccgg cagatgcatc accgccacca tggtccacaa ccacgacttc aagccctgcc
     1741 gggacacgac gtaacccccg atcagtccgc cgagcatcag catcacgacg ccgaccgtcc
     1801 cgtagacgat gccgacttcc gccgtggaaa ggccaagccc ccccgccgcg cgcgggtcga
     1861 gcagaaaggg gacgaccatc ttcatgagct gcgcctcggc gaagcggtaa aagagtagaa
     1921 aggcgatcac ggcggcgatg tccctcttac cgaagaagag ggcgaagcta ttgagaaaat
     1981 cggcaaagaa gttcttcgac aggccgcgcg gggcgggacg gtcggacgcg ggatagggaa
     2041 ggacgaaaaa atggtagagg aaagaggcga gaaaaaaacc cgccagcaca aaaaaagtcg
     2101 ccgaccaggc ggcggcgacg gaggggcagc ggcgctccag aagacccgcg aggatcacaa
     2161 gcaacccctg agcggagatc atggcgatac ggtagaacat attccgcacg ccgatgaagg
     2221 cagcctgctc acgctgagag agcccgagca tgtagaaccc atcggcggcg atgtcgtgcg
     2281 tcgccgagct gaaggccatc agccagaaga cggcgagggt ccagcggaaa aaatcgggga
     2341 cggggatggt caaggccacg gcggcaaggg acgccccgag caggacctgc atcgccagta
     2401 tccaaaaccg ctttgtccga aacatgtcca ctagggggct ccagaagggt ttgattaccc
     2461 agggcaggta gagccagctc gtgtagaggg cgatgtcggt gttcgatatg ccgagacgct
     2521 tgtacagcac gaccgagacg gtcatgacta ccgcgtaggg gatcccttcc gccaggtaga
     2581 gtgagggcac ccaggtccag ggattacggt atgagggttt ggggtgcttc attcaaggtc
     2641 agtacagctt tttcaactcg gcgccgcgga agtagtgtcg cagtatctct tccgccttgt
     2701 gccccttcaa ggccatgacg gcggccccga cctggcagag accgaccccg tggccccacc
     2761 cggcgccgcg caggatgaat ttcgccggcg tacccgcgcc gtcccgctcc aggccgataa
     2821 cgaaggcgct gctgagaagg tggctctccg aaagccacct gcggatctcg agctccttac
     2881 cgacgaccca ggacctctta gacccttcaa tgcggaggcg cactatacgt cccgaaggtc
     2941 cccgctccag ggggatcaac gcccgaagga tcccgaaatc gatccccgac tttttctgta
     3001 agatcgcctc gagctcttcc cgggtgtaag tcacttccca gcggaaaaat cgaccggtct
     3061 ccaggtcgga ggaagggagc acccgccgca ggacatcctc atcggtcacg ttacagtatg
     3121 cgtcggggtt ggaaaggatc catcgctcgg catcatcctc ggaaactacc gggctaaagg
     3181 gatggcgggc gtcggagacg gaagacagat agggaaccgc cctaccctcc cacacgtttt
     3241 cgtatttttc cgtcaacccg ccgcaggcct tgtggaatcg ggcgtcgcag acctcgccct
     3301 cgtggacgag gaacaggccg cgcgtctctt ccaccgccgc ggccgcccgc gccgggatgg
     3361 cctttgcgac cccccggtaa cgctggcagt ggtcgtcggc gcagacgtcg aagaggtcgt
     3421 gcgcctcccg gtcgtaccag cgaacggtct cgccttctcc gtcgatttct cccgacgcag
     3481 gcggcacctc tttccttgcc gcccgctcca acatggcggc aagccagctc cgggaagtaa
     3541 tagcctgtgc cttcagaaac tccggcggcc cttcggggtt catctccgaa ggaacgacgc
     3601 tggagaggta ggcctcgagg ccgacctcgt tggctgccgt aacggcgcct ccctcaccgg
     3661 cgaggaggat aaggttacct tcgaaggtct gttcttcttt atgttcccag tgaaatcggc
     3721 ttccgaccgc gacgtcgaac agggtgaacg ttgcgccggc cttcggcgtg cagcggattt
     3781 ccctgccctt cgctatttcc cgcccggaag aatcgaacag ggcgacgagg ccatccacgg
     3841 gttgcgactt gaaaaaacct gaaacgggcc ggcccgcgac gaggcaggac ccgtcaatcc
     3901 tgcctcgcat cacacggcaa cgatccagaa tgccgaccct gatcgtcggc tcaccgttta
     3961 tcatcaataa ttctcggcca gcacctcata ataggactga ggatgtctac aggcgggaca
     4021 tattttgggg gcactcgtcc cttcagcgac gtaaccgcag ttcctgcagt gccacttgac
     4081 gggggacttt ttcgcgaaca cctcgttgtt cgccacgttc tcggcgagct tccggtaccg
     4141 ctgttcgtgg aatttctcga ccttcgcgac ctgttcgaaa gaaatcgcga cctcggtgaa
     4201 accctcctcc ctggctatgg cggcgaagtc cgcatagagg gtcgtccatt cgaggttctc
     4261 cccagcggcg gcggcctcga ggttgctctt cgtgtctgcg atgactccgg ccggatagga
     4321 ggcgctgatc gcgacgtccc caccttcgag gtacttgaag aagacctcgg cgtgctcctt
     4381 ttcgttttcc gccgtctcca ggaagaaggc ggcgatctgt tcgtagcctt ctttgcgggc
     4441 ggcgctcgcg aaataagtat aacggttcct cgcctgtgat tcacccgcaa aagccgcgag
     4501 caggttcttt tccgtcttcg tgcctttcag cgatttagcc atttcttgac ctcctctggt
     4561 tatgttgtta gctaaggaaa ttcgctaaag aaaagattcg atgggtagcc attttccgct
     4621 tcatgtcgaa ggacgcttat gaagccggag gatttcttat accttaatcc gccgctcttc
     4681 gcaagaaaag gcaaccggtc catcatccac cgcttcgttg acagggccgc aaaaatgccc
     4741 gatgatgcaa agcgcacaga gactatgccg gctttcagaa tctcggcggc gtcatattac
     4801 gataagtgat gggcatggca aagaggaaac cttctatcgt cgtcggcgta gacctcgccg
     4861 ggtcgcccag ccgcccgacg ggcgtctgca tcctccgggg tctgaaggcg caaacatccg
     4921 tcgtcttcgg cgacgaggaa atcatcgagc tcataaaaaa taccggaccc gacctcgtaa
     4981 ccgtcgacgc cccgctgagc ctcccggaag gacggacaac gatccacgac cgctcgggcg
     5041 gccattttcg cgagtgcgac cgcgagctcc gtcgccgcgg catccgcttc tttccggtca
     5101 ccatcggccc gatgcggatg ctgacggagc gaggcatggc gctgaaaaca aaggtcgaaa
     5161 ggatggactg tcccgtgatg gagtgctacc ccggcgccgc ccaggacgtg tggggcatcc
     5221 cccgtcaaca ccgggacaga aaaggcctcc gggaaggatt gaaagacctg ggggtcaaag
     5281 gcctggcgga agccctgacg ggcgacgaac tcgacgcagt caccgcggcc ctcgtcggac
     5341 gctggttact tctcggcaag ggtgaaatca tcggcgggag cgacggcatc gtcatgcccg
     5401 cgagagagaa gaccgaagcg cggagaggac ggcggagcag gggtcaaatg tgtaatcacg
     5461 cctgagccat gccatggtga cccctttgga ccgtgaaggg tgatataaca tgaaccggcg
     5521 atttttattg aaaaatgcgg cacattttgc cataaaagtt tcatacaact tttccctagc
     5581 atcccatatg atgatgaact cgcaaaaagt cgagaattac ctctttcgtc actccggcga
     5641 acgctggaat ccagtatatt caatatgttc tggatgcccc cgtatcgagt acggggcagg
     5701 cttaatcgag tccggcatga cgtttttgga ctttttacgg ggacgtcata tgatgaagcc
     5761 ttctacaacc ctctgcctgt cctattgaat ctgggggacc atttatgaaa gacggatgca
     5821 agaccaggca gcaactcctc gatgaactga gtaaactcga gcagcgcatc ctgagcttag
     5881 agagggtgaa caagaaactc aagaaagata aaaccgggtt ggagcaggac tcaaaaaggt
     5941 tgcgtctcat attcgacaac gcacccgtgg gcgtctacat gagcacaccg gaagggcgtt
     6001 atctcaaggt caatcgtgcc caggtcgaca tactcggcta cgaaagcccg gaagagatgg
     6061 tccgttccgt cacggacatc aagaaccaga tctatgtgga acccgaagac cgggaccgcc
     6121 tcgagaagct cctcgcggaa cagggcatag ttaagggttt cgagacccgc tactatagaa
     6181 aagacggtga gattatctgg gtgtccattt acatccaggc tgccaaggac gaaaagggca
     6241 aggtctttta tcaaggaact gtcgtagaca tcacagagcg aaagcaagct gaggaagcct
     6301 tacgggaaag cgaaagaagg tacaggcatc tcgtcaattt cgtacccata cccttgttcg
     6361 agatcgacct cgcgggcaac ttcaagacgg gcaatttagc catttctgaa gccttcgggt
     6421 acacagaaaa agatgtcgag aaggggctca acatcgccca ggtgataata ccggaagata
     6481 ttgaacgtat cgtctccgac atgaaagaag tcctgcgtaa aggaaagata aggggttccg
     6541 ggcccggata cgagtacacg gggataagga aagacggcag cacatttccc tttattgcct
     6601 acgccagccc tatctttcac ggtgatgatc ctgtgggatt ccggggggca atcatcgacc
     6661 tcacggcgca aaggcaggcg gaggaagctc taaggaagag catctcaagc ctggcggagg
     6721 cccagcggat cgcacatatc ggaaactggg aatgggatgt tggcagcgat gacgtttact
     6781 ggtccgacga actttgccgg atcgttggaa cgtcgcctca ggcgttttcg ggaaatttcg
     6841 cggcattttg cgagctgttc ccctccgaag acagggaaac ggtgaaagtc gccgtcagaa
     6901 aagcagtaaa ggaggggcgc aagagcgaca tagaccatcg catcacccgg cctgacggca
     6961 cggtgcgtga cctccatcag ctggtagaac ccatcatcga cagggacggg aagaccatca
     7021 gggtcatagg actcgtgcag gacgtaaccg agaagaggca gcgggaaagg gaactgcaga
     7081 atgctaagga acagttgatc caggcggaaa aactctcctc cctgggcaga ctatccgccg
     7141 gggtcgccca tgaggtcctc aatccggcca ccatcatttc catggaactg cagctcttgc
     7201 aaaaaatggg ggacctcccg cctcacgtcc aggaagaact gaatatctgt atggagcaaa
     7261 tcagccgcat cgtcaccatc gcggaaaatc tcaaacagtt ttcccggatt cccgtaaaac
     7321 atacggctat gaccgatgtg aacaacctca tcgagcatga gctgaatcta tacgcgatgc
     7381 agctgaagat cgagggaata gaaacggacg tgcaatacga ccccgacctg ccatccatcc
     7441 ctatcgacag ggaaaagatt gagcaggtga tcatgaacct gatcacaaat gccgtctccg
     7501 cgatggaagg gaaggagaaa aaggtgctgc gcatcaagac gggcaggaac actgtgcata
     7561 ggaaaggcgt atgcctgaca attaccgtcg ccgacaccgg ccatggcatc tctgatgaac
     7621 atatgccgaa gatattcgac ccctttttta ccaccaagga accgggaaag gggacgggac
     7681 tggggctccc aatatcatac ggcatcgtcc aggagcacgg cggcatgatc tgggcggaga
     7741 acaacgagtg gggcggggcc accttctatg tctcgcttcc cattcagagg gacagtttga
     7801 gaaacaacgc gtgagatggg aggtgtatcc atggagagaa ttcttgtcgt cgatgatgaa
     7861 gcccagattg tgaggttgct gcaaaagtac ttcgcctcaa aaggatatga ggtctctacg
     7921 gccggaagcg gcctggaagc catcgaaaaa gtgaaggacg taaggcccca cgtggtgttg
     7981 ctcgacatcc tgatgccgga catgggcggc atagatacac tcaaggagat caaaaaaata
     8041 gacccccaaa tcggcgtgat catgatcaca gccgtcatcg acgaagagat agctagacga
     8101 gcgctggaac tgggtgccga cgattatatc accaagccca tcgatttcga ttacctcgag
     8161 acatgcgtca tggtgaaatt ggcgcaactt ccggggtgac cggataaatt gccgccgata
     8221 cctcgcagac gcgacaagct catttgaagc agtttgtggt tattccccga cgcctgccca
     8281 tctcttggcg atttcgattc tcttttcgag gtcataggcc gacctcacga cgatctcggt
     8341 tgtcatggtc gaccccgagg cgttaatcgc ccaacccaca aagtatcccg tccacctgga
     8401 ggcagctact tcctgcgcaa ggtaaagaac cctgagccgt tcttccgtcg tatacttggg
     8461 gttgccggcc aaataggtgt ccatccacgc ctttattgtt ggattcttgt agtcctgctc
     8521 cgtcggcatc gttacgatga ccccgccggc gatgtcatgc accaatgcga tatgctctcc
     8581 cgacaggttt ttgatgtaat gcagccctgc attcccgatg agagcgtttg gaatccatac
     8641 cccactcggg tggacctcgc cttccacggc ggcgccgact gcacatccat aggcagtctc
     8701 cgcctccatc atcagtttag tggccttgtc ccttatgtgg gccgtcttca tgcccaggcc
     8761 gtttacgtcg gcgataaggg ccacgatacc acagatcaaa tcggtgtggc ccgaaaggca
     8821 ggcacacttg gaatgacgtt gaatagacgc aaaataatgg ggtgcccggc ccgtcatgtc
     8881 ccattcgccg cagagaaaaa ccctctccca ggggacgaag acgtcatcga agaccgtcat
     8941 cgcctctacc gctccgatcg cgctgccgat aggacattcc atctgtgcgt ctgtgcgtga
     9001 cctattcggt gcaggcctcg cgacgaaagt aacacccttt gtgtcgaccg gcatcgagca
     9061 gcagacggca tagtctttat cggcctcttt gagctggcgg cacggaacga ccaccagctc
     9121 cctcatacaa ggaccataag atgtatgaac cttggcaccc ctgatgacga taccccgcga
     9181 attcttcttg acgacacgaa ggtcggtagg ggttttctgt tgggagggca tgagcgaccg
     9241 gtcgcctttg gggtccatca tcgcccaacc gatgtcgtag tcgttcttca tgagatactt
     9301 gacgtattct ttgaaacgtt catggtagca cgtgccatgt ttctggtccg tatcgtaggt
     9361 cgccgcccat actacgcaca attcattgga ctggcaccac gtacagatcc gcctctgggt
     9421 aacttccctt gtcagccgga ccttcgtgat ggcatcctct cggctcgtct gtatgtgcat
     9481 gttcaatcgc cggacgggct catcgacgag aggggagtgg acgaccgata gatgcttgaa
     9541 ctcctcatcg aaagaaaaat cgtgggtcgc tcctatgagg ttcaacgtag attgaaacct
     9601 cgggtcatcc cagacccggt cgaccttttc tcccagcatg taaatgtcgg gcttgactcc
     9661 tttcaggtct tcgacgtatt cagcctttgt tttgatagcc atgacatcct cctgcggtga
     9721 cgaccccgca atatgattta agcagcctta acggccttca aggattcgat cctgcttatg
     9781 atcgtcttta gcgacgaccc cggaccgaat acctcggcta ttccagcctt cttcagcttc
     9841 tcaacgtcgc ccggcggcac cgtacccccc aacacgacct tgatgtctcc agccttctcc
     9901 tttttcagca aattcacgac ctccggtgcc aggatcatgt gatttcccga aagggaactc
     9961 aggccgatca cgtccacacc ttcctgaagg gccgtcctca cgatattggc cggcatctga
    10021 ttgccgccgt agatcacctc catcccggcg tcccgcaacg ccctggccac gaccatgctc
    10081 ccccgccagt gggcatccat cccgaggcgg accatcagta cgcggatcct ttgcttttcc
    10141 atttatttct ccttaggtcg aattaggtta gaatagaggc ggttcccaca caccgtaaac
    10201 acgcttcagt gtccgagata tttcgccttc cgtgatcagg tttttgactt cccccacgat
    10261 aagctccatc aggttctcat tcctttcgca gcaacgggcg acggcgtcca gggatttctg
    10321 cacctttcgg gggtctcttt tctcccggat acgccgcagt ttctcctttt gcaccttgag
    10381 tgtttccggc gaagagaaga gctcgatcgg taggacctcg tcctcgatct ggaaacaatt
    10441 caccccgacg atcggcatct cccccgattc gatgtcacat tgatacttgt aggcagcgtc
    10501 cgagatttct gcatggatcc acccgctctc gacagaactc accagacctc ccttcccctc
    10561 gatttcatcg agaatcttga ggacctccgc ttccatccgg tcggtcagcg actccaggta
    10621 gtaggatcct ccaagcgggt cgatcgtatt gattacgccc gtttcgttct ggagaatctg
    10681 ctgcgtcctc aaggccgtaa ccgaggacag ttccgtcggg gtacacaacg cctcgtcata
    10741 ggcgtccaca tggaccgact gggctccgcc gagcacggcg gcgagcgcgt ggtaggccga
    10801 ccgggcgatg ttgttgagag gctgctgggc ggtcagggcg accccggccg tctgaacatg
    10861 gaagcggaac atgagagacc tcgggtcctc cgccttgaaa cgctgcctca ttatgttgta
    10921 gtaaacccgc cgggcggccc ggtactttgc gatctcttca aaaaaatcat tgtgagccga
    10981 caggaagaag ct
//
LOCUS       DAOTMX010000012        10620 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_5850_length_10620_cov_3.781070, whole genome shotgun sequence.
ACCESSION   DAOTMX010000012 DAOTMX010000000
VERSION     DAOTMX010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 10620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10620
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5850_length_10620_cov_3.781070"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..2011)
                     /gene="ispG"
                     /locus_tag="P5573_00735"
     CDS             complement(110..2011)
                     /gene="ispG"
                     /locus_tag="P5573_00735"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HRT26624.1"
                     /translation="MRPPATTVKEEQRTRKHTRTVLLGDLPIGGEAPVRVQSMTTTDT
                     RDVESTIAQIKALEDAGCEIARVAVPDREAALVLHRIKEGISTPLIADIHFDYRLALK
                     AIEEGVDGIRINPGNMKADRVREVVSAAKDSPVVIRLGVNSGSLEKDILSKHGGPTAA
                     ALVESALRSVRFIEDLGFDRLKVSLKSSHVPTMVAAYRGLAEKTDYPLHLGVTEAGTL
                     LRAAIKSAAGIGSLLIDGIGDTIRVSLTEDPEFEIPVALALVRRYEERKGRDEGRGTR
                     DETVGLSAFAEASADGGWSLPYSPYDYSRRETREVLHVGGKQVPRVIADYSKKETITP
                     ASLFGVGYQYSVPLDKWNLTDQACDFLYTGDQVLDFEIPGTLGIIVNSGTFARLENKE
                     RYYPLFCADEYFRAKKRSERLNFVVIDRKTLTENSAFDLQRLDATVVLVVDTMAEHGM
                     AEERDAIRILMERSCPCPVILKRNYANLSPEQLQLFSSTDLGGLLLDGMGDGIWIKAP
                     GVASEATINSTGFGILQATRTRISKTEYISCPSCGRTLFDLQETTAKIRARTDHLKGV
                     KIGIMGCIVNGPGEMADADYGYVGTGPGKITLYKGREVVKRNIDEAEAVDQLIGLIRE
                     HGDWVEIGR"
     gene            complement(2008..3195)
                     /locus_tag="P5573_00740"
     CDS             complement(2008..3195)
                     /locus_tag="P5573_00740"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRT26625.1"
                     /translation="MRLSERVKCIKPSPTLAITAKAQSMRAAGRNIINFGAGEPDFDT
                     PDHIKKAAISAIEAGFTKYTPVGGIDELKDAIIAKLKHDNGLEYGRPNIFVSCGAKHT
                     LFNLAQVLFEAGDEVIIPAPYWVSYPDIVVLSGATTVVISTTEEDGFKLKADDLARAV
                     TSKTRAVIINSPSNPTGMAYSEAELKALAEVVLEKDLIVISDDIYEKIAYEGFRFANI
                     AMTGKEIKERTIVVNGVSKAYSMTGWRIGYAAGPQDVISAATDYQSQNTSNPTSISQK
                     AAAAALKGDQTPAATMVKEFQKRRDYTVDTLNGIKGIRCMKPQGAFYAFPNTADLYGK
                     SFRGRRVEGSVDLAAYLLDEAEVAVVPGEPFGSDEHIRLSFATSMENIEEGLKRIRQA
                     LEG"
     gene            complement(3192..3707)
                     /gene="coaD"
                     /locus_tag="P5573_00745"
     CDS             complement(3192..3707)
                     /gene="coaD"
                     /locus_tag="P5573_00745"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HRT26626.1"
                     /translation="MERVAVYPGSFDPITNGHLDIIRRGLKFFDKMIVLVAYNPSKAG
                     LFTVEERIAFIKTVIGDEPRITVDSSAGLLVEYVKQCKANVVLRGLRALSDFEYEFQM
                     ALMNRRLNREVETVFLMTGFKWFYTSSNLIKEAASHGGSVRGLVPDIVLRSLEKKYGR
                     RLNNNEGEKQR"
     gene            complement(3592..4269)
                     /gene="rsmD"
                     /locus_tag="P5573_00750"
     CDS             complement(3592..4269)
                     /gene="rsmD"
                     /locus_tag="P5573_00750"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HRT26627.1"
                     /translation="MRVIGGSAKGHRLAKPPGVVLRPTADMVKEALFNILADVTGKRF
                     LDLFAGAGGVGIEAASRGAAKVVFVDKNRRCTETVFRNLRSCGIEGGHECLAMEVGRA
                     LRLLSKKEEQFDVIFLDPPYEEGLVEKTLQDIVRSNILREDGCVSIQHSVREPVEEKA
                     GGLISERERRYGDTVLTFMKFEQRRCVHGKSCGLSRFVRPHHERPSGHHQEGFEVFRQ
                     DDRPCRL"
     gene            complement(4297..5316)
                     /locus_tag="P5573_00755"
     CDS             complement(4297..5316)
                     /locus_tag="P5573_00755"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HRT26628.1"
                     /translation="MRNYHVAVVGATGAVGNEMISVLEERNFPVGKLTLLASERSIGK
                     RLDFRGESYPVQVLKEDSFKGVEIGLFSAGGGISQRFAPIAAEAGCVVIDNTSAFRMD
                     PEVPLVVPEVNPTDIGDYRKKGIIANPNCSTIQMVVVLKPIHDRARVRRVVVSTYQAV
                     SGTGKKAMQELEDQTRNILNFREPEIRVYPHQIAFNCLPHIDIFLENGYTREEMKMVN
                     ETKKIMGDYSIAVTATTVRVPVFRGHSESVNIETERKITVQEVRGLMEKAPGVKLVDD
                     PARNVYPLAIDAAGKDETFVGRIREDESIPYGINLWVVSDNLRKGAALNAVQIAEVLA
                     RDYLR"
     gene            complement(5533..6360)
                     /gene="pssA"
                     /locus_tag="P5573_00760"
     CDS             complement(5533..6360)
                     /gene="pssA"
                     /locus_tag="P5573_00760"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HRT26629.1"
                     /translation="MAEMKSRQRFAARMRGRRRFNRDRMKKGIYVLPNLFTTASLLAG
                     FYSIVMAMYGKFTRASVAILIAWVLDGLDGRMARLTNTTSKFGAEYDSLADLVAFGVA
                     PAILVYVWSLSPYGKWGWMAAFLFVVCGALRLARFNVQVNIIDKRLFNGLPIPAAAVV
                     VASTVILFHHLGVEGRFNHVGILFAVAAVSLLMVSSVKYYSFKDLNYFTRKPFISFVL
                     IVLIMMIVIAEPQITIFTFSLGYSLSGPAWFILKLIRRKGGAFAKEEAKDIEAGAKS"
     gene            complement(6348..6992)
                     /locus_tag="P5573_00765"
     CDS             complement(6348..6992)
                     /locus_tag="P5573_00765"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HRT26630.1"
                     /translation="MKRKDSMIVGEGYPFIVPPAALTVVFAYAGLFWLTTLCSVLTLF
                     VVWFFRNPDRAAPSGEGVFVSPADGRVLKVEDVPRGENLTGPFRKVSIFMNVFDVHVN
                     RAPCSGTVTAIHYHKGRFLSANLDKASELNEKNTVVIRREEGEDVLVSQIAGLIARRI
                     VCWVGEGVHVERGDRIGMIRFGSRVEVFLPQDAKVSVEAGTRVKAGETIIGWLR"
     gene            complement(7020..7244)
                     /locus_tag="P5573_00770"
     CDS             complement(7020..7244)
                     /locus_tag="P5573_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT26631.1"
                     /translation="MEKSDEALIARYVDRDEELRKYVDEHRKYEEALEAFNKRVYLTP
                     EEEFEKKKIQKLKLQGKDRIQHILARYRER"
     gene            complement(7336..8040)
                     /gene="tsaB"
                     /locus_tag="P5573_00775"
     CDS             complement(7336..8040)
                     /gene="tsaB"
                     /locus_tag="P5573_00775"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HRT26632.1"
                     /translation="MLTLAVDTSSATESVALLADREILAERTARVLTNHAASLIPSID
                     SILSSCGVKISNIDLYALTVGPGSFTGLRIAASTIKGLAFSSGKPMVGVSTLEALACN
                     AVPTPYPVCPLMDARRKEVYAALYRIRDNGYPERLLADRVVAPEKFIANTPERTLFLG
                     EGARKYSQVIADLNPSGVVFGKPGSEFVRAANVGLLGVDLYFRGQTLDLITFSPAYLR
                     LPQAEEEKVGRSPQYS"
     gene            complement(8053..9117)
                     /gene="rseP"
                     /locus_tag="P5573_00780"
     CDS             complement(8053..9117)
                     /gene="rseP"
                     /locus_tag="P5573_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HRT26633.1"
                     /translation="MIGINIVSVTILLGILIFVHELGHFLAAKMFGVGVLKFSLGFGK
                     KLVGKKVGETEYMISLIPLGGYVKLLGESESDVIAPEDERRSFLKQHVLKRIGIVSAG
                     PLFNFLFAVLLLAALNMVGIPTPSSDIGDVQSGSAAEKAGLKPGDRILAIDGKETVTW
                     GDVAKIIEKSQGRAVPVRYLRGGEERETTVQPQLTMAKNIFGEDVEAYKIGIIGSSNV
                     VVRSLNPLAALWAGTGQTWAMTELTVLSVVKLIQGVISPSTLGGPILIAQMAGAQVKK
                     GIMPFVLFMAILSVNLGVLNLMPIPILDGGHLLFFLVELVTGREISVKWREWAQQVGF
                     AILILLMLFVFYNDIMRIFS"
     gene            complement(9114..10313)
                     /locus_tag="P5573_00785"
     CDS             complement(9114..10313)
                     /locus_tag="P5573_00785"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HRT26634.1"
                     /translation="MGESEARQGTRMKNISILGSTGSIGVSALEVVRRNPARFRVLGL
                     AAGRNVELLSRQVEEFRPMYVSLFDKETAAEFKKLAGATSTAILWGEEGYRDIATIEG
                     VEMVLSAMVGAAGLLPTVCAIEAGKDVALANKETLVMAGGLVMEKASRKGVRILPVDS
                     EHSAIFQCLEGERREYVKKIILTASGGPFRTLPPEKMAEVSVEDALKHPNWKMGKKIT
                     IDSATMMNKGLEIIEAQWLFGVDVDRIHVVVHPQSIVHSMVEFCDGSVIAQLGVPDMK
                     GPIAYALSHPERLEREEGFLDFYAIGTLDFAAPDMGRFPCVRLAYEAGRKGGTMPAIM
                     NAANEVAVEAFLGGKIGFTDIAAVIEKTMQGLESREAGSVEDILDADRRARKFAAAYV
                     EGAARRT"
     gene            complement(10321..>10620)
                     /locus_tag="P5573_00790"
     CDS             complement(10321..>10620)
                     /locus_tag="P5573_00790"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HRT26635.1"
                     /translation="SQGKTVEGAIGSIAGSIVGCLIFRAWFFPELAVHHAMIMGLVGN
                     MVGELGDLCESVLKRSAGVKDSGTIFPGHGGVLDRLDCILFIGPFIFYYRWLTTG"
BASE COUNT         2126 a         3273 c         2740 g         2481 t
ORIGIN      
        1 acctcgcatc tttttttcct cgtccctcgg tctcggtctc ggtctcgcgt cttccccttg
       61 tctcacgtcc ctcgtccctc gtccctcggt ctcggtctcg ttcctcgcct cacctcccta
      121 tctccaccca atccccatgc tcccgaatca gcccgatgag ctgatccacc gcttccgctt
      181 cgtcgatgtt gcgtttcacg acttcgcggc ctttgtagag ggtgatctta ccggggccgg
      241 tgcccacata cccgtagtcg gcatccgcca tttcacccgg accattcacg atgcatccca
      301 tgatgccgat cttgacgcct ttgagatgat cggtacgcgc gcggatcttc gccgtcgttt
      361 cctgtaagtc gaacaaggta cgtccgcaac tcggacagga gatgtattcc gtcttcgaga
      421 tgcgtgtacg tgtcgcctgc aggatgccga agccggtgct gttgatcgtc gcttccgacg
      481 ctacacccgg tgccttgatc cagatgccgt cgcccatgcc gtcgagcaaa agcccgccca
      541 ggtcggtgga ggagaagagc tgtaactgct ccggagataa gttggcgtag ttgcgcttca
      601 ggatgaccgg acaaggacag ctgcgctcca tcaggatccg gatcgcgtcg cgctcttccg
      661 ccatgccgtg ttccgccatg gtgtcgacca ccagcacgac cgtcgcatcg agccgctgga
      721 gatcgaatgc ggaattttct gtaagtgttt tccgatcaat gacaacgaag ttcaaccgtt
      781 cggaacgctt cttcgcccgg aagtattcgt cggcacagaa gagcggatag tagcgttctt
      841 tattttccag ccttgcaaag gtcccggagt tcacgatgat ccccagcgtg ccggggattt
      901 caaagtcgag tacctgatcg ccggtgtaca ggaaatcaca cgcctgatcc gtcaggttcc
      961 acttgtcaag cggaaccgaa tactgataac ccacaccgaa caacgaagcc ggcgtgatcg
     1021 tctctttctt gctatagtcc gcgatcaccc gcggcacctg cttgccaccc acatgcaaaa
     1081 cctcccgcgt ctccctccgc gaataatcat aaggcgaata aggcaaactc catccaccat
     1141 ccgccgaagc ttcagcgaag gcggacagtc caacggtctc gtccctcgtc cctcgcccct
     1201 cgtccctccc ttttcgctcc tcgtacctcc taaccaacgc cagtgcgaca ggaatttcga
     1261 actcgggatc ctccgtcaac gatacccgga tcgtatcgcc gatcccgtct atcagtagcg
     1321 accctattcc ggcggcagat ttgatcgctg ccctgaggag cgtcccggcc tcggtgactc
     1381 cgagatggag ggggtagtcc gtcttttccg ccaggccgcg gtaggcggcg accatagtgg
     1441 gtacatggga cgacttgagc gagaccttca acctatcgaa ccccaggtct tcgataaaac
     1501 ggacggacct caaggcgctc tcgacgaggg ctgccgccgt cggccccccg tgcttcgaga
     1561 ggatgtcttt ttccaacgaa ccggaattga ctccgaggcg gattactacc gggctgtcct
     1621 tggcggccga gacgacttct ctcacccggt cggccttcat attcccgggg tttatcctta
     1681 tgccgtcgac accctcttct attgccttaa gggcgaggcg gtaatcgaaa tggatgtcgg
     1741 cgataagcgg agtagaaata ccctccttta tcctgtgcaa cacaagggcc gcctcgcggt
     1801 cgggaacggc gaccctcgcg atctcgcaac ccgcatcttc gagggcttta atctgggcga
     1861 tggtcgattc gacgtccctg gtatcggtgg tcgtcatgga ctggacgcgg acaggggctt
     1921 ccccgcctat cgggaggtcg cccaagagga cggttctggt gtgttttctt gtcctttgct
     1981 cttccttcac tgtcgtcgcg ggtggcctca accttcgagg gcttgtctta tcctcttcaa
     2041 tccctcttct atgttctcca tcgaggtcgc aaaagacagt cgaatatgtt cgtcgctgcc
     2101 gaagggttct ccggggacta cggccacctc ggcctcgtca agcagatagg cggcgaggtc
     2161 gaccgagcct tccacccgcc ttcccctgaa ggacttcccg tacaggtcgg cggtgttcgg
     2221 gaaggcgtag aaggcgccct ggggtttcat acatcggatc ccctttattc cgttcagggt
     2281 gtcgactgtg taatcccgcc ttttttggaa ctccttcacc atcgtcgccg cgggcgtctg
     2341 gtcccccttg agagcggcgg cggcggcctt ctgggagatg gaggtgggat tagacgtgtt
     2401 ctggctctga taatcggtgg ctgccgagat gacgtcctgc ggtcccgccg catagcctat
     2461 ccgccatccc gtcatggagt aggcctttga caccccgttc accacgatcg tcctctcctt
     2521 tatctctttg cctgtcatgg cgatgtttgc gaacctgaag ccttcgtagg cgatcttctc
     2581 gtagatgtcg tcggagatga cgatcaggtc cttttccagg actacttcgg caagggcctt
     2641 taattcggcc tccgaatagg ccatcccggt ggggttcgag gggctgttga tgatgacggc
     2701 ccttgtcttg cttgtgaccg ctcgtgccag gtcgtcggcc ttcagcttga atccgtcttc
     2761 ttctgtcgtg gagatgacca ccgtggtcgc gcccgagagg acgacgatgt cgggatagga
     2821 gacccaataa ggcgcgggaa tgatgacctc gtcgccggcc tcgaagagga cctgggcaag
     2881 gttgaaaagc gtgtgcttcg ccccgcagga cacgaatatg ttgggccgcc cgtattcgag
     2941 tccgttgtcg tgcttgagtt ttgcgatgat ggcgtctttg agttcatcga tgccgcccac
     3001 cggcgtatac tttgtgaacc ccgcttctat ggccgagatc gccgcttttt tgatgtggtc
     3061 gggggtgtcg aaatcgggtt ccccggctcc gaagtttatg atgtttctac cggccgcccg
     3121 catggactgc gccttcgccg tgatggccag ggtcggagac ggctttatac attttaccct
     3181 ttctgagagc ctcatcgctg tttttcccct tcattattgt tcagccttct tccatatttc
     3241 ttttcgagac tgcgaaggac gatgtccggc acgagtcccc ggacggaccc gccgtgactt
     3301 gccgcttctt tgataaggtt cgaactcgtg tagaaccact tgaaccccgt catcaggaag
     3361 acagtctcta cttctcggtt gagcctgcgg ttcatcaacg ccatctggaa ctcgtactcg
     3421 aaatcggaga gggcccggag tcccctcagg accacattag ccttgcactg cttcacgtac
     3481 tccacgagaa ggcccgccga gctgtcgacg gtgatccttg gttcgtcacc gatcacggtc
     3541 tttatgaagg cgatcctctc ttcgacggtg aagagccctg ccttgctcgg gttataggcg
     3601 acaaggacga tcatcttgtc gaaaaacttc aaacccctcc tgatgatgtc cagatggccg
     3661 ttcgtgatgg ggtcgaacga accgggatag accgcaactc tttccatgaa cgcaccttct
     3721 ctgctcgaat ttcataaagg ttaataccgt atcgccgtat cttctttctc tttccgaaat
     3781 cagcccgccc gccttctctt cgacaggctc cctaacagaa tgctgaatcg atacacaacc
     3841 gtcttcgcgg aggatgttcg atcggacgat gtcctggaga gttttctcga ccaatccctc
     3901 ttcgtaaggc gggtcgagaa aaatgacgtc gaactgctct tccttctttg aaagcagtct
     3961 cagtgccctg ccgacctcca tggccaggca ttcatggccc ccttctatcc cgcaacttcg
     4021 gaggttccgg aagacagtct cggtacacct gcggtttttg tcgacaaaga ctacttttgc
     4081 ggcgcccctg cttgccgcct ctatcccgac gccccccgcg ccggcgaaga ggtcgaggaa
     4141 cctcttgccc gtcacatcgg caagtatgtt gaatagggcc tctttgacca tgtccgccgt
     4201 cggtctgagg acgacgccgg gcggtttcgc cagccgatgc cccttcgcac tgcccccgat
     4261 cactctcatc ctgacttcct tggacggccg tccaccctat ctcaagtagt cccttgccag
     4321 gacctcggcg atctgcaccg cgttgagggc tgcacctttt ctcaggttgt ccgatacgac
     4381 ccagaggttg atgccgtagg gtatcgattc atcctctctt atcctaccga cgaaggtctc
     4441 gtcttttccc gcggcgtcga tggcgagcgg atagacgttt cgtgcggggt cgtccacaag
     4501 tttgaccccc ggcgcctttt ccatcaaccc ccgcacctcc tggacggtta tcttcctctc
     4561 cgtttcgatg ttgacggatt ccgagtggcc ccggaaaacg gggaccctga ccgtcgtcgc
     4621 cgtgactgct atcgaatagt ctcccatgat cttcttggtt tcgttcacca tcttcatctc
     4681 ttcacgggtg tagccgtttt ccaggaagat gtcgatatgg gggaggcaat tgaaagcaat
     4741 ctggtgggga tagaccctta tctccggttc ccggaagttg agaatgttgc gcgtctggtc
     4801 ttccagctcc tgcatcgcct ttttccctgt tccagacacc gcctggtatg tcgagacgac
     4861 taccctcctg accctggccc tgtcgtggat aggcttcagg acgacgacca tctggatcgt
     4921 cgagcaattg gggtttgcga tgatcccttt cttcctataa tcgccgatgt ccgtcggatt
     4981 aacctccggg acgaccaggg ggacctccgg gtccatccgg aaggcgctgg tattgtcgat
     5041 gaccacgcag cccgcctcgg ccgctatcgg cgcaaatcgt tggcttatcc cgcccccggc
     5101 ggaaaaaagc cctatctcga cgcctttgaa ggaatcttct ttgaggacct ggacaggata
     5161 tgactcgccc cgaaaatcga gtctcttgcc gatggaccgt tccgacgcca acagggtcaa
     5221 tttccccaca ggaaaattcc gctcctccag gacgcttatc atttcattac ccactgcacc
     5281 agtcgcgccg acgacggcaa catgataatt tctcatagta aactcattac cccttccgat
     5341 ttaactgtat gctaactttt gatggactcg taaaaagtcg agaattacgc gtttcgtcat
     5401 tccggcgaac gccggaatcc agtatattca atatgttctg gatgcccccg tatcggagcc
     5461 tgccccgtac ccgatacggg gagcaggctt aatcaagtcc ggcatgacgt ttttggactt
     5521 tttaccagga cgtcaacttt tggcacccgc ctcgatgtct ttggcttctt ccttcgcaaa
     5581 ggcaccgccc ttccgcctta tcagcttcag aatgaaccag gcaggcccgg aaagactgta
     5641 accgagagaa aatgtgaaga tcgtgatctg cggctccgct atcacgatca tcatgatcag
     5701 gactatcagg acaaaggaga taaaaggttt cctggtgaag taattcaggt ccttgaagct
     5761 gtaatacttt acactgctca ccatcagcaa cgacacggcc gctacggcaa agaggattcc
     5821 cacgtggttg aaccgaccct cgacccccag gtggtggaac aggatgaccg tcgaagccac
     5881 tacgaccgcg gccgcgggga tcgggaggcc gttgaagagc ctcttgtcga tgatgttcac
     5941 ctggacgttg aacctggcga gcctcagcgc cccgcaaacc acgaagagga aggccgccat
     6001 ccagccccac ttcccgtaag gggataaaga ccaaacgtaa acgaggatag cgggggcgac
     6061 accgaaagct accaggtctg ccagggaatc gtactcggcg ccgaacttgc tagtggtgtt
     6121 ggtcagcctt gccatccttc cgtccaatcc gtccaggacc caggcgatca agatggccac
     6181 ggaggcacgg gtaaacttcc cgtacatggc catcacaatc gagtagaacc cggccaacag
     6241 cgaagccgtg gtgaacagat tgggcagcac gtatatacct ttcttcatcc tgtcacggtt
     6301 gaaacgtctc ctgcccctca tcctggccgc aaaccgttgc ctgcttttca tctcagccat
     6361 cctattatag tttcaccggc ctttaccctg gtacccgctt cgacggagac cttcgcgtcc
     6421 tgtgggagaa agacctcgac cctcgaaccg aaacggatca tacctattct gtctcctctt
     6481 tcgacatgaa ccccttcccc gacccaacac acgatccgtc tggctatcaa cccggcgatc
     6541 tgggagacca atacgtcttc gccctcttcc ctcctgatga cgaccgtgtt cttttcgttc
     6601 aactcggatg ccttgtccag gttggcggaa aggaaccttc ccttgtggta atggatcgcc
     6661 gtcaccgtac cggaacaggg cgcccgattg acgtggacat cgaaaacatt catgaaaatg
     6721 ctgaccttcc ggaacgggcc ggtgaggttt tccccccgag gcacatcctc aaccttcagc
     6781 acccgtccgt ccgccggcga aacgaaaacg ccttcgccgg aaggcgccgc cctgtcggga
     6841 ttccggaaga accagacaac gaacagagta agcacggaac acagggtggt aagccagaaa
     6901 aggcccgcgt aggcaaaaac gacggtcagt gctgccggag gaacgatgaa cggatagcct
     6961 tccccgacga tcatgctgtc tttacgtttc ataaaaggcc atgcggcggc gccgactgtc
     7021 tatctttcgc ggtaccgggc gaggatgtgc tgtatcctgt cctttccctg gagtttcaat
     7081 ttctggatct ttttcttttc gaattcttct tccggcgtca gatagaccct cttattgaag
     7141 gcttccagcg cctcctcgta ttttctgtgc tcgtctacgt actttctgag ttcttcgtcc
     7201 ctatcgacat atcgggcgat caaggcttcg tcactttttt ccatacaccc atcctcctgc
     7261 atgtttcgac ttatattaga tatacgtccg attgtcaacc tcaagcattc ataagatatg
     7321 aagttttatg gctttctacg aatattgcgg acttcttccc accttctcct cctcggcctg
     7381 gggcaaccgc agataagcgg gcgaaaaggt gatcaggtcc aaagtttgcc ccctaaaata
     7441 caggtcgacc ccgagcaaac cgacgttcgc cgccctgacg aattcgctgc ccggtttgcc
     7501 gaagacaaca cctgaggggt tcaggtcggc gatgacttga gaatactttc gggcgccttc
     7561 tcccaggaac agcgtccttt ccggggtgtt tgcaatgaac ttttccgggg ccacgaccct
     7621 gtcggccagt agccgctcgg ggtagccgtt atcgcgaatc cggtaaaggg cggcgtagac
     7681 ttctttcctc ctcgcatcca tcaaaggaca gacaggataa ggcgtcggca cggcattgca
     7741 ggcaagggcc tccagggtcg aaacgcccac catcggtttc ccggaagaaa aagcgagccc
     7801 ttttattgtg cttgcggcga tccgcaagcc ggtgaaggaa ccgggaccga ctgtcaacgc
     7861 atagaggtcg atattcgaga tcttcactcc gcaggacgac agtatactgt ctatggaggg
     7921 gatcagagac gccgcatggt tggtcaatac ccgggccgtc ctttcagcga gtatctctct
     7981 gtcggctaac agggcaacgc tttcggtagc ggacgacgtg tcaacggcca gggtaagcat
     8041 agaatcgact catcaactga aaattctcat gatatcgttg tagaagacga agagcatgag
     8101 gagaatcagg atggcaaaac cgacctgttg ggcccattcc cgccatttga cgctgatttc
     8161 tctgccggtc accagttcga ccaagaagaa aaggagatgc ccgccgtcga gtatcgggat
     8221 gggcataagg ttgaggaccc ccaggttcac actcaggatc gccatgaata agacaaaggg
     8281 catgatcccc tttttgacct gggcaccggc catctgggcg atcaatatcg gcccgccgag
     8341 ggtcgaggga gagattacgc cctggatcag cttgaccacg ctgaggacgg tcagttctgt
     8401 catagcccat gtctgtcccg tacccgccca cagtgccgct aggggattga ggcttcttac
     8461 gactacattc gacgaaccta tgatcccgat cttgtaagcc tccacatcct cgccgaagat
     8521 gttcttcgcc atggtaagct gtggctgaac cgtggtttcc ctttcttcgc cgcccctcag
     8581 atagcgcaca gggacagccc tgccttgact cttctcgatt atcttggcta catcgcccca
     8641 ggtaacggtt tctttcccgt cgatcgccag gatacggtca ccgggcttca aacccgcctt
     8701 ttcggccgcc gaaccactct gaacatcgcc gatgtcggac gagggggtcg gaatgccgac
     8761 catgttcaaa gctgccaaga ggaggacggc aaaaagaaag ttgaacaaag gccctgccga
     8821 aactatgccg atgcgcttca gtacgtgctg tttcagaaag gagcgccttt cgtcttccgg
     8881 ggcaatgacg tcgctttccg attcgccgag cagcttgacg tagcctccca acggtatcag
     8941 agaaatcatg tattccgtct cgcccacctt cttaccgacg agctttttcc cgaagccgag
     9001 agaaaatttc aataccccca ccccgaacat cttcgccgcc aggaaatggc cgagttcatg
     9061 gacaaagatc aggatgccca ggagaatggt gactgaaact atgttgatgc ctatcatgtc
     9121 cttcttgccg ccccctcgac gtaagccgca gcaaactttc tcgccctccg gtctgcgtcc
     9181 aggatgtcct cgaccgaacc tgcctcgcgg ctctccaaac cctgcatagt cttttcgata
     9241 accgccgcga tatccgtgaa tcctatcttt ccgccgagga aggcctcgac ggctacctcg
     9301 ttcgcagcat tcatgatagc cggcattgta ccaccttttc tgcctgcttc ataggcaagt
     9361 ctcacgcagg gaaatctacc catgtccggt gccgcgaagt cgagggtgcc gatcgcatag
     9421 aaatcgagga acccttcttc ccgttcgagc ctttctgggt gcgaaagggc gtaagctatc
     9481 ggccctttca tgtcgggcac tccgagttgg gcgatgaccg aaccgtcgca gaattcgacc
     9541 atggagtgga cgatgctctg cgggtggacg actacgtgga tcctgtcgac gtcgacgccg
     9601 aacagccact gggcctcgat gatttcgagt cccttgttca tcatcgtggc cgagtcgatc
     9661 gttatctttt tccccatctt ccagttgggg tgcttcaggg catcttccac cgacacctca
     9721 gccatcttct cgggcggcaa cgtcctgaag ggcccccccg aagccgtcag gatgatcttc
     9781 ttcacgtatt ccctgcgctc tccctccaag cactgaaata tggcgctgtg ttcgctgtcc
     9841 acaggaagga tccgcactcc ctttcgtgag gccttttcca tgacaaggcc cccggccatg
     9901 acgagtgttt ccttgttcgc gagcgccacg tccttgcccg cctcgatcgc gcataccgtc
     9961 ggcaacaacc cggcggcccc caccatcgcc gaaaggacca tttctacacc ttcgatagtg
    10021 gctatgtccc tgtacccttc ctctccccac agaatggcgg tcgaagtagc tccggccaat
    10081 tttttgaatt ccgccgctgt ttccttatcg aagagggaaa cgtacatcgg cctgaattcc
    10141 tcgacctgtc gcgacaggag ctcgacgttc cggcctgcgg cgagtccgag caccctgaac
    10201 cgcgcggggt tgcgccgcac tacttcgagg gcgctcacgc cgatcgaccc ggtcgaaccc
    10261 agtatggaaa tattcttcat cctcgtgccc tgccttgcct ccgattcgcc catcgacaaa
    10321 tcaacccgtc gtcagccagc ggtagtaaaa gataaaaggc ccgatgaaca ggatacagtc
    10381 cagtcggtcc agtacccctc cgtgtccggg gaatatagtc cccgaatctt tcacacccgc
    10441 cgacctcttg aggaccgact cgcataggtc gccgagctcg cccaccatgt taccaacgag
    10501 tcccatgatc atcgcatgat gcacggccaa ctcggggaaa aaccacgcac gaaagatcag
    10561 gcaaccgact atactcccgg cgattgaccc gatcgcgcct tccaccgttt tgccctggct
//
LOCUS       DAOTMX010000013        10479 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_5949_length_10479_cov_3.499520, whole genome shotgun sequence.
ACCESSION   DAOTMX010000013 DAOTMX010000000
VERSION     DAOTMX010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 10479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10479
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5949_length_10479_cov_3.499520"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..986)
                     /locus_tag="P5573_00795"
     CDS             complement(<1..986)
                     /locus_tag="P5573_00795"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRT26636.1"
                     /translation="MRSDLMKKGLERAPHRSLFKAMGYTDSEIARPMIGVVNSANEII
                     PGHVHLGRIAEDVKAGIRTAGGTPVEFPAIGVCDGIAMGHVGMKFSLASRELIADSVE
                     VMAMAHPFDGLVFVPNCDKIVPGMLMAAMRLNIPSIFVSGGPMLAGDMGGKKVDLISV
                     FQGIGQVKARTMTVDELKALEDCACPGCGSCSGMYTANSMNCVTEALGLGLPGNGTIL
                     AVNAARRRLAKEAGVRVMDLVKKKVKPRDIATLEAFKNAIAVDMALGCSTNTVLHIPA
                     IAHEAGIRLSLDLFNRISEKTPNICKLSPAGEHHIEDLDAAGGIQAVMKEISR"
     gene            complement(1017..2081)
                     /gene="ilvC"
                     /locus_tag="P5573_00800"
     CDS             complement(1017..2081)
                     /gene="ilvC"
                     /locus_tag="P5573_00800"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HRT26637.1"
                     /translation="MAKIDFGGVVEDVITSKEFPLKKAQKVLKGETVAVLGYGVQGPG
                     QALNLRDNGIRVIVGQRKGTESWRRAVKDGFKPGETLFPLEEAAARGTIVCYLLSDAG
                     QKTMWPKIKPHLTEGKALYFSHGFSITYGRLTKVVPPPDIDVILVAPKGSGRTVRSNF
                     LDGSGINSSFAVFQDYTGRALERTLAMGIAIGSGYLFPTTFENEVYSDLVGERGVLMG
                     CLAGVMEAQYNCLRSHGHSPSEAFNETVEELTQSLIRLVAENGMDWMYASCSTTAQRG
                     ALDWRGPFRDAVAPLFEKLYKRVVAGTETRIVLKANSAPDYRERLEEELAELRNSEMW
                     RAGAAVRALRPERRKKAASK"
     gene            complement(2098..2589)
                     /gene="ilvN"
                     /locus_tag="P5573_00805"
     CDS             complement(2098..2589)
                     /gene="ilvN"
                     /locus_tag="P5573_00805"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF008864.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HRT26638.1"
                     /translation="MEKQKKEHILSLLVNNKPDVLARVAGTLGGRGYNIDTLCVDATL
                     DPKISKIILSVYQDQAAITKIEKQLLRLIDVHSVSELTETETVMREMMLVRMRLTEKN
                     KADIALIIDTFRGRVVTMNGDSCVIEITGTKADIERAIESLKPLGIDDLARTGIVALE
                     RIK"
     gene            complement(2612..4312)
                     /gene="ilvB"
                     /locus_tag="P5573_00810"
     CDS             complement(2612..4312)
                     /gene="ilvB"
                     /locus_tag="P5573_00810"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HRT26639.1"
                     /translation="MKLKGAEIFIRALKQEGIQTVFGYPGGVLLDIYDELHRSDITHI
                     LVRHEQGAVHAADGYARASGKVGVVLVTSGPGATNTVTGIANAYLDSIPIVVFTGQVP
                     TALIGTDAFQEADIVGITRPCTKHNFQVRRVDELARTIHEAFHIARSGRPGPVLVDLP
                     KDVMQATTDYEPSPPKEEEPVQPPRPASGKALYVLDLIAESRRPVLLTGGGIVLGRAS
                     EELRQMARRTKIPVTSTLMGLGAFPASDPLWLGMPGMHGTYYANMALSNCDLLIAIGC
                     RFDDRVTGNLETFARKAKKVHVDVDASSINKNVMVDVPIVQDCKEALRSFNKLLADQN
                     FTVSDEDRREWFDMIDEWKRNVPLSYCPSDEVIKPQFVIETLCNLTKGKAIITTEVGQ
                     NQMWAAQFYKFDEPNMFISSGGLGTMGFGLPAAIGAQVAFPDKLVVDIAGDGSIQMNI
                     QELATAVQYNIPVKVVVLNNGYLGMVRQWQELFYEKRYSQTDMTYAPDFVKVAEAYGM
                     LGVRAVKPDEVEATLRKGLEHPGPVIMDFRVAREECVYPMVRPGGSIQEMALGREMIN
                     "
     gene            complement(4692..7073)
                     /locus_tag="P5573_00815"
     CDS             complement(4692..7073)
                     /locus_tag="P5573_00815"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HRT26640.1"
                     /translation="MKKIRNGARKSPWFRRFFLFILFLFPFLVVSAGLYLYVIIRDLP
                     DIHNLKDYRPSITTRIYSDNDRLIDEFFLEDRRFIPINKVPKYVAQAFIAAEDSRFFQ
                     HEGVDFYGMVRAFVKNVTHGAIVQGGSTITQQLAKAIYLSPERSLTRKIKEAVLAYRM
                     DRYLKKYEILNLYLNQIYLGHGTYGIEAASQCYFGKSARDLNLAEAALLAGLPKAPSR
                     YSPFVSLEKAKQRQAYTLNRMVEEGYITEAEKERAEKMPIRLNTAGPKGKVAPYFTEN
                     VRRYIVAKYGSEALYKEGLEVYTTLNFEMQQAATAAVERGLQELEKREGTRVSIRRVP
                     AAKVDQFTAQVEKEISKKPLEKGQVVRALVESVDEGDRVVHLRVGRRKAVASFDHIHL
                     AANAVQAAEMGKMYNPAGLFSPGDVVEARLSEKPQKEPLELSIELHPAVQAALLCMDA
                     RTGEIKAMVGGRDFRRSEFNRATQSRRQPGSAFKPLIYAAAFDKGLTPATIVMDSPVV
                     FKDSLGDTDWRPKNFDAKYHGPTTLRDALVYSRNVVTIKVLKDIGVRYAVDYARNMGI
                     ESPLSENLSLALGTSGVTVQEMVRAFGVLANEGKKAEPYFIRKIVDRTGSIFEEHAPR
                     VEPVIDAKVAYITTSVLQDVVQRGTGWRVRAIGKPVAGKTGTTDEYKDAWFIGYTPSF
                     VAGVWVGYDDGRPLGKAEVGGVAAAPIWLYFMEKALAGQPVENFRVPEGIVFVKIDPK
                     TGLLASPFSSDARTECFLEGTAPTEYAPSDLERWFMKNFGGAPAKESEKEDLD"
     gene            complement(7094..7867)
                     /gene="tatC"
                     /locus_tag="P5573_00820"
     CDS             complement(7094..7867)
                     /gene="tatC"
                     /locus_tag="P5573_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HRT26641.1"
                     /translation="MSDNPENDKMPLTAHLEELRKRLLRSLIAAFIAFGICYNFKERL
                     FEVLTRPLVESIPKNSYMIYTSLPEAFFNYLKISFFAGLVVASPYILYQIWKFISPGL
                     YASEKKHVVPFVLTSTILFVSGVLFGYFLALPPAFKFFLEFSTDFLKPMLTLREYLSL
                     SLKLLLAFGIVFEIPVFIFFLAKIGVVNAALLRKQRKYAILLIFIVAALITPTPDAFS
                     QTLMAVPMMILYEVGIIVAKWGEKKTAGGEAKEAPEGLS"
     gene            complement(7924..8241)
                     /gene="tatA"
                     /locus_tag="P5573_00825"
     CDS             complement(7924..8241)
                     /gene="tatA"
                     /locus_tag="P5573_00825"
                     /inference="COORDINATES: protein motif:HMM:NF011430.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HRT26642.1"
                     /translation="MNIGLPELIVILIVALLVIGPKRLPDVAQALGRGLAEFKRAMED
                     VRDELKVDEVKRDIDDVKDSLLRKGHEEEKVTATHSGEDGHEAHPPQSTSPGGQKPSA
                     EGS"
     gene            8442..9500
                     /locus_tag="P5573_00830"
     CDS             8442..9500
                     /locus_tag="P5573_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HRT26643.1"
                     /translation="MPLRTGIVKDERYMRHETSAFHPESPKRLEAIYRMLESPDMKGK
                     FVLIEPRYASPEEIGMIHEQWYIEVVAKTAGKPHTYLDPDTETSPESYDTARLAVGGL
                     CNAVDAVVKDEVDNAFAFIRPPGHHAEADRGAGFCLFNNVAIGAMHAINKLGMKKILI
                     VDWDLHHGNGTQHSFYDDPRVVYFSTHQYPYYPGTGSVQEIGRGQGKGFTVNVPLRTG
                     PGDNEYVKIFRSILQPIALEFKPDLVMLSAGFDIYFKDPLGGMKVTPKGFACLTRIIL
                     NIADACCDGRLAVTLEGGYHVGGLTDSCKTVLNEMRDDTHDTEDELKRIEAEANPYID
                     KIIEAVKDQLRPYWKALQ"
     gene            9572..9859
                     /gene="gatC"
                     /locus_tag="P5573_00835"
     CDS             9572..9859
                     /gene="gatC"
                     /locus_tag="P5573_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HRT26644.1"
                     /translation="MKITKEEVEYVAHLARLEFSGTETEKFTSQLNDILLYMEKLNRV
                     NTDGVEPLTHAATLSNAFREDRVLPSLPHDLSLANAPDRKANNFRVPKVIE"
     gene            9868..>10479
                     /locus_tag="P5573_00840"
     CDS             9868..>10479
                     /locus_tag="P5573_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HRT26645.1"
                     /translation="MKLHELTVHELQQKLRRGETTAAEVVESVIGRIDEVEDKTRSYI
                     TVLRDSAREEALRADEEIRQGRARPLTGIPVAVKDIICTKGVRTTCGSRILHDFIPPY
                     DATVVERLRGAGALFIGKTNMDEFAMGSSTETSYFGVTRNPWDLERIPGGSSGGSATA
                     VAADECIASLGSDTGGSIRQPAALCGVVGLKPTYGRVSRYCLVA"
BASE COUNT         2146 a         3124 c         2898 g         2311 t
ORIGIN      
        1 cggctgattt ctttcattac cgcctggatg ccgcccgccg cgtcgaggtc ttctatgtga
       61 tgttcgccgg cgggactgag cttgcagatg ttaggcgtct tctcgctgat gcggttgaaa
      121 aggtcaaggc tcagccggat gcccgcctca tgggcgatgg cggggatgtg gaggaccgtg
      181 ttggtagagc atcccagggc catatcgacg gcgatggcgt tcttgaacgc ctccagcgtg
      241 gcgatgtccc ggggcttgac ctttttcttc acgaggtcca tgaccctcac ccctgcctcc
      301 tttgccagac ggcggcgcgc cgcattgacc gcaagaatcg tcccgttgcc gggcaggccg
      361 aggccgagcg cctcggtgac acaattcatg gagttcgccg tgtacatccc ggagcaggat
      421 ccacagccgg gacaggcgca gtcttccaag gccttgagtt catcgacggt catcgtgcgg
      481 gccttgacct gtcctatgcc ctggaagacg ctgatcaagt cgaccttctt tcctcccata
      541 tccccggcca acatcgggcc gccgctcacg aagatcgacg ggatgttgag gcgcatggcc
      601 gccatcagca tccccgggac gatcttgtcg cagttgggga cgaacacaag tccgtcgaag
      661 ggatgggcca tcgccatgac ctcgacggaa tcggcgatca actcgcggct cgcgagagag
      721 aacttcatcc ccacgtgacc catggcaatg ccgtcgcaga cgccgatggc cggaaactcg
      781 accggcgttc cgcccgccgt ccggataccc gcctttacgt cctcggcaat ccggccgagg
      841 tgtacgtgcc ccgggatgat ttcattagcc gagttcacaa ccccgatcat cggcctcgca
      901 atctcgctgt ccgtgtaccc catggccttg aaaagcgacc ggtggggcgc tctctccagg
      961 ccttttttca tcaagtcgct gcgcatatca gtcccctttc cgccgacggc cgatcactat
     1021 ttgctcgccg ctttcttcct tctctcaggc ctcagcgccc gtaccgccgc acccgcccgc
     1081 cacatctcgc tgttacgcag ctccgccagc tcctcttcca gtctctcccg gtagtcgggt
     1141 gcactattag ccttcaggac tatccgtgtc tctgttccgg cgacgacgcg tttatacagt
     1201 ttttcgaaga gaggggccac ggcgtcacgg aaaggccccc tccagtccag ggcacctcgc
     1261 tgggccgtgg tgctgcaact ggcgtacatc cagtccatgc cgttctccgc cacaagacgg
     1321 atcaggctct gggtcagctc ttcgaccgtc tcgttgaatg cttcactcgg actatggccg
     1381 tggctgcgca agcagttgta ctgggcctcc atgacacccg ccaggcaacc catcaggacg
     1441 cccctctcgc ctacgaggtc gctgtagacc tcattttcga aggtcgtggg gaatagataa
     1501 ccagaaccga tggcgattcc catggccagg gtcctttcca gcgccctccc ggtgtagtcc
     1561 tggaagacgg cgaaactcga gttgatgccg ctgccgtcca gaaagttgct ccgcacggta
     1621 cgcccggaac ctttcggcgc cacgaggatg acatctatgt ccggaggcgg aacaaccttc
     1681 gtcagcctcc cgtacgtgat ggaaaagccg tgggaaaaat acagcgcctt tccctcggtg
     1741 aggtggggct tgatcttcgg ccacatggtt ttctggccgg catcggaaag aagatagcag
     1801 acgatggttc cccgtgccgc tgcttcttcg aggggaaaga gcgtctcgcc cggcttaaaa
     1861 ccgtccttca ccgcccttct ccaactttca gtccccttcc gctgcccgac gatgacacgg
     1921 atgccgttat ccctaaggtt cagtgcctgt cccggcccct ggacgccgta acccagtaca
     1981 gccactgtct cgcccttcag taccttctgc gccttcttca ggggaaattc cttcgaggtg
     2041 atgacatcct cgacgacccc accgaaatct atctttgcca ttcttcttac ctccttttta
     2101 ttttattctc tccagggcaa cgatgcccgt ccgggccaag tcgtcgatcc ccagcggttt
     2161 gaggctctcg attgcccttt cgatgtccgc ttttgtaccc gtgatctcga tgacgcagct
     2221 atctccgttc atcgtgacga cccgccctcg gaaggtatcg atgatgagcg caatatccgc
     2281 cttgttcttt tccgtgagcc tcatcctgac gagcatcatc tcgcgcatga cggtctccgt
     2341 ctccgtcagt tcgctcacgg agtgtacatc gataaggcgg aggagctgtt tctcgatctt
     2401 cgtgatcgcc gcctggtcct ggtagacaga caggattatc ttcgaaatct tcgggtccag
     2461 ggtggcgtcc acacagagag tatcgatgtt gtacccccta ccccccaggg taccggcgac
     2521 tctcgctaaa acatcgggct tattgttcac aagtagcgac aagatatgtt cttttttctg
     2581 cttttccatt ttcagattac ctcgctccga atcaattgat catttcccgt cccagggcca
     2641 tctcctggat cgacccgccc ggcctcacca tggggtagac gcactcttca cgggcaaccc
     2701 ggaaatccat gatgaccggc cccgggtgtt cgagtccttt cctgagagtc gcctccactt
     2761 catctggctt tactgccctc acgccgagca tcccgtaggc ctccgccacc ttgacgaagt
     2821 ccggggcgta ggtcatgtcc gtctgggaat aacgcttttc gtagaacagt tcctgccatt
     2881 gacgtaccat ccccaggtag ccgttgttga gaacgacgac ctttaccggg atattgtatt
     2941 gaacggccgt cgccaattcc tgaatgttca tctggatact cccgtcaccg gcgatgtcca
     3001 caaccagctt atccgggaac gcaacctggg cgccgatggc ggcgggcagg ccgaacccca
     3061 tggtcccgag gccgccggac gaaatgaaca tgttgggctc gtcaaacttg tagaactggg
     3121 cggcccacat ctgattctgt ccgacctcgg tcgttattat ggcctttccc ttagtaagat
     3181 tgcaaagagt ttcaatgaca aactggggct tgatgacctc atcgctggga caataagaga
     3241 ggggaacgtt cctcttccat tcgtcaatca tgtcgaacca ttccctgcgg tcttcgtcgg
     3301 aaacggtgaa gttttgatcc gccagcagct tgttgaaact ccgcaacgcc tccttgcagt
     3361 cctgcacgat aggaacatcg accatgacgt tcttgttgat cgatgaggcg tccacatcca
     3421 cgtgaacctt ctttgccttg cgggcgaagg tctcgagatt acccgtgaca cggtcgtcga
     3481 aacggcagcc gatggcgatg aggaggtcac aattactgag ggccatgttg gcataataag
     3541 ttccgtgcat gcccggcatc ccgagccaga gagggtctga tgccgggaac gccccgagcc
     3601 ccatcagcgt cgacgtgacg ggtatcttcg tccgccgcgc catctgtctc agttcttccg
     3661 acgcccggcc gaggacgatg ccgccgccgg tcagtagaac gggccttctc gactcggcga
     3721 tcaggtcgag gacgtacagg gccttaccgg aagcaggtcg cggcggctga accggttcct
     3781 cttccttggg tggagacggt tcataatcgg tcgtcgcctg catgacgtcc ttgggcaggt
     3841 ccactaggac cggccccggc cgccccgaac gggcgatgtg gaaggcctcg tgtatcgtcc
     3901 tggctaattc atctacacgc ctcacctgaa aattgtgttt ggtgcatggt cgggtaattc
     3961 ccacgatatc ggcttcctgg aaagcgtccg tgccgatgag tgccgtgggg acctgcccgg
     4021 taaagaccac gatgggaatg gagtcgagat aggcgttggc tatgcccgtg acggtattcg
     4081 tcgcgcccgg cccggaagtc acgaggacga caccgacctt tccggaggcc ctggcatatc
     4141 cgtcagccgc gtgtacggcg ccctgctcgt gcctgacgag gatatgggtg atgtcgctcc
     4201 ggtgtagctc gtcatagatg tcgagaagaa cgcctcccgg atagccgaag accgtttgaa
     4261 tgccctcctg tttcagggct ctgataaaaa tttctgcgcc tttcagtttc aatgaaaaca
     4321 cctccttgtg gtattaacaa aatcgccctc agcggtttcc cgctgccctt ggaccacgcc
     4381 tcacggcgac agccatcaca acagaccggc ggtccctgac cctcaaggct tggagggcat
     4441 ttaattcctc ggtaagctac cctgacgggt accccaaaaa aaaaccgtca ccagggctgg
     4501 taacggtttt tggttttttg ccggagaaag atcaaaccat cacgcagccc ctgtcatcgg
     4561 ggatctacaa ctacagctac gactacgatg gtgagcgtga tgatttttct catacggata
     4621 taccgttagc ttctacaaaa aagaacatcg acctgtcaag tattttttca gtattttttc
     4681 agtcgatgtt ttcagtccag gtcttccttt tcactttctt tcgccggagc gcccccgaaa
     4741 ttcttcataa accatctttc caggtcgctg ggtgcgtatt ccgtgggtgc cgtcccctca
     4801 aggaaacatt ccgttctggc atcggacgaa aaggggctcg cgaggagtcc cgtcttgggg
     4861 tcgatcttca caaagacgat cccctccggg acgcgaaagt tttccacggg ctgccctgcc
     4921 agggccttct ccatgaaata cagccagatc ggcgcggcgg ctacgccacc cacttccgcc
     4981 ttgccgagcg gcctgccgtc gtcgtacccg acccagaccc ccgcgacgaa ggagggggta
     5041 taaccgataa accaggcgtc cttgtactca tccgtggtcc ccgtctttcc cgccacgggt
     5101 tttcctatag ccctcacccg ccatcccgta ccgcgctgga cgacatcctg gagcacggaa
     5161 gtcgtgatgt aggccacctt ggcgtcgatc accggctcta ccctcggcgc gtgttcttcg
     5221 aagatactgc ccgtacggtc gacgatcttc ctgatgaaat agggctcggc ttttttccct
     5281 tcgttcgcca gtacaccgaa ggccctcacc atctcctgga ccgtgacgcc cgacgtccct
     5341 aaggccaggg aaagattctc ggaaagcggc gattctatgc ccatgttgcg ggcgtaatcg
     5401 acggcgtaac gcacaccgat gtccttgagg accttgatag taaccacgtt gcgggaatag
     5461 accagggcat cccgtaacgt agtgggacca tggtatttgg cgtcgaaatt cttcggccgc
     5521 caatcagtat cgccgaggga atccttaaag acgaccgggg aatccatcac gatggtcgct
     5581 ggtgtgagtc ccttgtcgaa ggcggcggcg tagatcagcg gcttgaatgc agagccgggc
     5641 tgccgccgcg attgggtcgc ccggttaaat tcgctccgcc tgaagtcccg tcctcccacc
     5701 attgccttga tctcgccggt cctcgcgtcc atacaaagaa gggcggcctg aacggccgga
     5761 tgaagctcga tggaaagctc cagcggttcc ttttggggtt tttcgctcag gcgggcctca
     5821 accacatccc cggggctgaa gaggccggcg gggttgtaca tcttgcccat ctcggcggcc
     5881 tgaacggcgt tcgctgccag gtggatgtgg tcgaaggacg ccacggcctt ccggcgcccc
     5941 acccgcagat ggacgaccct gtcgccctcg tctaccgatt cgacaagcgc cctgacgacc
     6001 tgcccttttt ccaaaggctt ctttgaaatc tccttctcca cctgggcggt gaactggtca
     6061 accttcgccg cggggaccct cctgatggac acccttgtcc cttctctctt ttcgagctcc
     6121 tgcaaccccc tctccacggc tgccgtggcg gcctgctgca tctcaaagtt cagcgtcgtg
     6181 tagacctcga gaccctcctt gtacaaggcc tcactaccgt acttggcgac gatgtaacgg
     6241 cgtacgttct ccgtaaaata aggggccacc tttcccttgg gaccggccgt gttcagcctt
     6301 atcggcatct tttccgcccg ctccttttcc gcctcggtta tgtagccttc ttccaccatc
     6361 cggttcaggg tgtaggcttg acgctgctta gccttctcga ggctcacgaa aggggagtac
     6421 cgactcggtg ccttgggaag tccggcgaga agggcggcct cggccaggtt taagtccctc
     6481 gcgctcttgc cgaaatagca ttgcgaagca gcctcgatcc cgtaagtccc gtgtccgagg
     6541 tatatctggt tcaggtaaag atttaatatc tcatatttct tcagataacg gtccatccgg
     6601 taggcgagga cggcctcttt gatcttcctc gtcaggctcc tctcgggcga gaggtagatg
     6661 gccttcgcga gctgctgggt gatggtgctg ccgccctgga cgatggcgcc gtgggtcacg
     6721 ttcttgacga aggcccgcac catcccgtag aaatcgaccc cctcgtgctg gaagaacctc
     6781 gaatcctcgg cggcgataaa ggcttgggcg acgtatttgg gtactttgtt tatcgggata
     6841 aaccgcctgt cctcgaggaa aaactcgtcg atcaaccggt cgttatcgga atagatacgg
     6901 gtcgtgatgc tcggccgata gtccttcagg ttatggatgt cgggcaggtc gcggatgatt
     6961 acgtacagat agagaccggc ggaaacgaca aggaagggga aaaggaagag gataaagaga
     7021 aaaaacctcc tgaaccaggg gctttttctc gcgccgtttc tgattttttt catcgaaaga
     7081 tcgccgtgcc gcctcatgac agcccttcag gtgcctcctt cgcctcgcct cccgccgtct
     7141 tcttctcccc ccacttcgcc actataatcc ctacctcgta gagaatcatc atggggacgg
     7201 ccatcagggt ctggctaaag gcgtcgggcg tcggcgtgat taacgccgct acgataaaaa
     7261 tcaacagaat tgcgtatttt ctctgcttcc tcaatagagc tgcgttgacg acgcctatct
     7321 tcgccagaaa aaagatgaag acggggatct cgaagactat gccgaaggcg agcaagagtt
     7381 tgagcgagag tgaaaggtat tcccgcagtg tcaacatcgg cttcaggaag tcggtcgaaa
     7441 attcgagaaa aaacttgaag gccgggggca gagcgaggaa ataaccgaag agaacccccg
     7501 aaacgaagag gatcgtcgat gtcaacacaa agggaaccac gtgctttttc tcggaggcgt
     7561 agagccccgg cgagatgaat ttccagatct ggtacaggat gtaagggctc gcaacgacca
     7621 gtcccgcgaa aaaagatatc ttaaggtagt tgaagaaggc ctcggggagg ctcgtgtaga
     7681 tcatatagct gttcttcggt atggattcaa ccaggggcct ggtcaggact tcgaaaagtc
     7741 gttccttgaa attatagcag atgccgaagg cgatgaaggc cgctatgagg cttcgcaaca
     7801 acctcttgcg caattcctca aggtgggccg taaggggcat cttgtcgttt tctggattgt
     7861 cgctcatatc tctgcttcta cggaacgcct tctcccgccg ttcttcatct aaatcttatg
     7921 ggattatgac ccttccgcag aaggcttctg accgccgggt gaggtcgatt gggggggatg
     7981 ggcttcgtga ccgtcttcac cggagtgagt cgcagtcact ttttcttctt catgaccctt
     8041 cctgaggaga gagtctttca cgtcgtctat gtcccgcttc acctcgtcca ctttcagctc
     8101 atctctgaca tcttccatcg ctcgcttgaa ctccgcgagc cccctgccca gcgcctgagc
     8161 cacgtcgggc agtcttttcg gtccgatgac gagcagggct actatgagga tgactatcag
     8221 ctccggcagg cctatgttca tgtcgagtac tcacgaaaaa tttagagatt gcaatatatc
     8281 taatgagaat ggtttgtcaa tgcatttgag ggcatctggg ttttcgcgca taacaggtaa
     8341 accctttact tttcgtgata ttgtatggta agtgagggca acttaccaga gaaagttttc
     8401 tccaccatag aaaaacgaga aagaacgagc ggaggtggga ggtgccttta agaacgggaa
     8461 tcgtcaagga tgaaaggtac atgcggcatg aaacgagtgc ctttcacccg gaatctccga
     8521 aacggctcga agcgatctac aggatgctcg aatctccgga tatgaagggt aaattcgttc
     8581 tgatcgagcc gcgatacgcc agtcccgaag aaatcgggat gattcatgag caatggtaca
     8641 tagaggtggt tgcgaagaca gcggggaagc cgcacaccta tctcgaccca gacacggaaa
     8701 catctcccga gtcgtacgat accgccaggt tggccgtcgg ggggttgtgc aatgccgttg
     8761 acgccgtcgt gaaggacgag gtcgacaatg ccttcgcctt catcaggccg ccgggccacc
     8821 acgccgaagc ggaccgcggg gccggattct gcctcttcaa caacgtcgct atcggggcca
     8881 tgcatgccat caacaaactg gggatgaaaa aaatcctgat cgtggactgg gacctccacc
     8941 acggaaacgg cacccagcac tccttctacg acgacccccg cgtcgtctat ttttccacgc
     9001 atcagtaccc ttactacccg gggacgggga gcgtgcagga aatcggcagg ggccagggaa
     9061 aaggcttcac ggtgaatgtc cccctccgga cgggaccggg ggacaatgaa tacgtaaaga
     9121 ttttccggtc gatccttcag ccgatagccc tcgaattcaa accggacctc gtgatgcttt
     9181 ccgccgggtt cgatatctat ttcaaggacc ccctcggggg tatgaaggtc acgccgaagg
     9241 gtttcgcctg tttgactcgc atcatcctca acatagccga tgcctgttgc gacggccggc
     9301 ttgctgtcac ccttgaaggc gggtaccacg taggcggcct caccgattcc tgtaagacgg
     9361 tcctcaacga aatgagggac gacacccatg atacggagga cgagttgaag cggatagagg
     9421 ccgaggcgaa tccctatatc gacaagatta tcgaagcggt gaaggatcag ctcaggccgt
     9481 actggaaggc cttgcaatag gcggcgactc gagacgggaa aaggaactcc atcaaggtga
     9541 ccggtgacgg tcggtagaac tgggggattt tgtgaagata acgaaggaag aggtcgagta
     9601 cgtggcgcac ctcgcccgtc tcgaattctc ggggacggaa acagaaaaat ttacgtctca
     9661 actgaatgat atcctgcttt acatggaaaa gctcaaccgg gtgaacaccg acggcgtgga
     9721 acctctcacc catgccgcaa ccctcagtaa cgccttccgt gaagacagag tcttaccctc
     9781 gctcccccat gacctatccc tggcaaacgc acccgacagg aaagcaaaca atttccgcgt
     9841 tcccaaggtc atcgagtaaa gagcgcgatg aaactccacg aactgacagt ccatgaactt
     9901 cagcagaaac tgcgccgcgg tgaaacgacg gcggccgagg ttgtcgagtc cgtcatcggg
     9961 cggatcgacg aggtagagga caagacccgt tcctacatca cggtgctgag ggattccgcc
    10021 agggaagagg cccttcgggc cgacgaagag atcaggcagg gcagggcaag accgctcacc
    10081 ggcatcccgg tcgccgtcaa ggacatcatc tgcaccaagg gcgtacggac gacctgcggc
    10141 tcgagaatcc tgcatgattt catcccgcct tacgacgcca cggtcgtcga gaggcttcgt
    10201 ggcgcgggcg ccctgttcat cgggaagacc aacatggacg aatttgccat ggggtcttcg
    10261 acggagacgt cctacttcgg agtgacgcga aacccctggg acctggagag gatacccggg
    10321 ggatcgagcg gcgggtcggc gacggccgtc gccgccgatg agtgcatcgc ctcgctcggt
    10381 tccgacacgg gagggtcgat ccgccagccg gctgccctct gcggggtcgt ggggctgaag
    10441 ccgacttacg gccgcgtttc gcggtactgc ctggtggcc
//
LOCUS       DAOTMX010000014        10438 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_5978_length_10438_cov_3.727246, whole genome shotgun sequence.
ACCESSION   DAOTMX010000014 DAOTMX010000000
VERSION     DAOTMX010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 10438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10438
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5978_length_10438_cov_3.727246"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="P5573_00845"
     CDS             complement(<1..379)
                     /locus_tag="P5573_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT26646.1"
                     /translation="MEHLQKELETKNRVIASVFRISNLLTQQILLDEILRSILMSAEK
                     DLGFSASCLFLVSDDRRHLACRMVRGFGAENEIRAYQRPFDLDRHDCIETRVVRDGRV
                     VYFEDSLRDPRATEIDRIITRKLG"
     gene            complement(826..2457)
                     /locus_tag="P5573_00850"
     CDS             complement(826..2457)
                     /locus_tag="P5573_00850"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HRT26647.1"
                     /translation="MISNGQNEKEYDLLIRGGHVYNGLGTPGQDVDVAVKGGRIVHLG
                     PGLEGRHAGRVIDASGLVVAPGFIDPHSHTDVELLVNPRAESKIRQGVTTEIGGNCGF
                     TFFPLNDESFQQRGRILKEKYDLDLDWRDIRGFFRRLEQGGIALNYATLLGHGTLREA
                     VVGCEDRVSEDHEVEEMKSIVEDCIEAGALGLSTGLIYTPGVFAQQEEIIDLCRVVAR
                     YGGVYSTHMRDEGDYLMEAVEETIKVATEARVSLQISHLKLAYPRNWSKIHAVLSRIS
                     EVKAEGFNIMADRYPYTATSTFLSVFFPRWAREGKITEYLKNSKGTVLEKKLRESIKA
                     QEEKIGSWDNIMITSVITQKISHLPGKSILQASREAGKDPYDFIRDLIIEEDDQVGMI
                     NFSLNEETFKRIILHPLVVIGSDGWALAPYGKLAFGKPHPRSYGTFPRMLGRYVRDER
                     LMPLGRAIEKMTSLTAEKFGLAGRGRIMNGYYADLVIFNPDTVIDLSTWDDPHRYSRG
                     IEYVIVNGEVVIDQGEHTGRLPGKVLRYLQRRVIL"
     gene            complement(2511..3182)
                     /locus_tag="P5573_00855"
     CDS             complement(2511..3182)
                     /locus_tag="P5573_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HRT26648.1"
                     /translation="MKGTLVNSGGIIVGSLIGLSVGNRLPERFKKTVMQTLGLSVVVI
                     GLQMALSVREVIPVVGCLLLGALTGELLEVEDWVEKLGEWLKERFHSGSATFVEGFVS
                     ASVLYISGAMVIVGAIQDGAVGDASTLYLKAMLDGVASVAFASTLGLGVAFSAIPVLV
                     VQGTITLLASQLLFLQDPRVLSAVTATGGVLILAIGINILELKKIPVGNLLPSLVYAV
                     LWALV"
     gene            3327..3794
                     /locus_tag="P5573_00860"
     CDS             3327..3794
                     /locus_tag="P5573_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT26649.1"
                     /translation="MKTHTFLFREGLWSVEGVYFDDRGRPIALEGKTRVTHLPRLWVN
                     EGAMELKSGEVPIVVHNRYEVTPFEEGRSTTSWKSSNPAVGELNGSFILVDDAILSVC
                     RSGDGLFGGSEFMLRVNDEQYLSRGAFLRENERLSSWSVTLRKIKDGPFPESV"
     gene            complement(3879..4154)
                     /locus_tag="P5573_00865"
     CDS             complement(3879..4154)
                     /locus_tag="P5573_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT26650.1"
                     /translation="MKKVLSTLSMAVFVVLVTGTFAIAEYSATGASNFPYFHLGALIV
                     GGLILISLRHKYNKLYMSEVMGAFALYTVLIALFTSPVVELIKSVVG"
     gene            4394..5278
                     /locus_tag="P5573_00870"
     CDS             4394..5278
                     /locus_tag="P5573_00870"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRT26651.1"
                     /translation="MNSIMEKVAPGIFLVPGENRSLFPFCTCLYLKGRNLRVLIDAGM
                     GDARMDACLREGIDVLILTHCHFDHQSTIGRVRGIPVWCHDVEVPYLEDRDVYLRETG
                     ILRSGIDIDRFFKRHPFPDLRVDRHLTDGERIDLGGLTLQVVHAPGHTPGHCAFYFPE
                     ANFLFSADVALNTFGPFYGNDFGSIEDFIASIRKLKALGASTVATSHSGPFRNRLAER
                     FSQYENIIYRRDELILKYLSGNKTLAGFLGKHVVYKDYPEPREVTMWMELVQVEKHLQ
                     RLMEKGRVRQEGDLYIKT"
     gene            complement(5356..6516)
                     /locus_tag="P5573_00875"
     CDS             complement(5356..6516)
                     /locus_tag="P5573_00875"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRT26652.1"
                     /translation="MDQKESKKTIRTYSWASFLNDMGSDMIYPVWPLFVTSLGANMAV
                     LGFLDGLGDAIVSLSQAASGYISDRISKRKVFVWLGYLFGGLSRLGYAFSTAWQHLIP
                     FRILDRAGKIRGAPRDAIIADLSTDEDRGKNFGLLRTMDNLGAVCGTLTCILFVGFLG
                     YRYLFLIAAVPSFIGAALIISVGREKKPDGSKVFKGIRLRDLDRNFRFFLLLSALFAL
                     GSFSYSFLLICAKEFGFEVTFVPVLYLAFTAVAAATSLPFGRLSDRIGRKAVMVIAFI
                     LWGLVCLGFMFVKSHLMVVANFILYGLHRGAIDTVQRAFVAELSPEAYRASGLGGFQM
                     VVGLCALPASVIAGLLWESIGVLAPFYFSLALTVVAAAMLMFVRGGVNGTEK"
     gene            complement(6550..6954)
                     /locus_tag="P5573_00880"
     CDS             complement(6550..6954)
                     /locus_tag="P5573_00880"
                     /inference="COORDINATES: protein motif:HMM:NF023514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3568 family protein"
                     /protein_id="HRT26653.1"
                     /translation="MNGPSRCAMVFLLALGAMILSGCAVMVGDKVVGIESGRFFYSDG
                     VLWTNYKASFEETWSACEKALADMKAGETTKKKKIGTGTIDSTIGDDKVHIALEYVSK
                     DTTSVGVRVGLAGNDIASKLIQEKIGANLQKN"
     gene            complement(7065..7901)
                     /locus_tag="P5573_00885"
     CDS             complement(7065..7901)
                     /locus_tag="P5573_00885"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRT26654.1"
                     /translation="MPNKEKKPIICPNCRKLINHDEERCPYCGIVGPGASWKRYLADS
                     IGSRDIVSVVFYANIAFYILSIVLDPRKIGVSANPFTFLSPSENSLFVLGATGTIPVG
                     LFHRWWTLVSASFLHGSILHIFFNMAALRQLGPFVMHEYGTGRFIIVYVLSGVVGFYV
                     SVLAGIPFTIGASASLCGLIGAILYYGKSRGGFYGQAVYKQALGWVIGLILFGLLVPG
                     INNWAHGGGIVAGALTGFFVGYEEKRAENQLHKILAAGLIVLTCLVLLWAVLQAIYFR
                     FY"
     gene            complement(7933..9093)
                     /locus_tag="P5573_00890"
     CDS             complement(7933..9093)
                     /locus_tag="P5573_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HRT26655.1"
                     /translation="MEHADIHRRSFNGKEVVLVGTAHVSKESADLVEAVIEGEKPDTV
                     CVELCKTRLEALKNPRKWLETDIVKVIREKRTSVLLSQLLMASFQKRMAEKFDIRPGE
                     EMHRAVVAAEKIGAEVVPADRDIRVTLMRTWRKMGCWSKFKLLPEAVASLIMAEEISE
                     EEIEKLKKRDALEVALNLFGQKLPEIKTTLIDERDEYLSHTIRHAPGRKVVAVVGAGH
                     IPGILKNEGKEADIEALNKVPPRGPWGDVVQWVLLAGVLGLIIGGFFYAGSQASFNMV
                     KSWVAITALSAAVGALAVLAHPLTIVTAAISAPVATIHPLIATGWIAGLAEASLRKPQ
                     VKDFLDLAEDITTVRGFWRNKITRILLLVVFVNLTASAGTLVAIPAMMHFLR"
     gene            9258..>10438
                     /locus_tag="P5573_00895"
     CDS             9258..>10438
                     /locus_tag="P5573_00895"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HRT26656.1"
                     /translation="MPDVMDARFRIGQTVEAMIETVAFGGDGVARVRDMVLFVPFTAD
                     GDLAEVRVVEVRKNFLRGKLRRLLIPSPHRTEPECPYYLRCGGCRYQHISYEHQLELK
                     RNQVAESFKRIGRFSSPPVEDIRPSPKSYNYRGKVEIHLRTKRGYPPEIGFIDTGGLR
                     IVDVERCAIADETINNALRELRGKALSGDLPIGGEETKILWSGDQDMNEDGPITRVVK
                     GRKLEVHRQGFFQANLSLTDALVDAVLDLAALTGGERVLDCYCGVGLFSLFLAAKAGD
                     IIAVDIDREAVGAARKNFAAFGHGGVRLFEGSVEDILREHLAGEKGRTDVVVLDPPRT
                     GCDKVLLDTLAEWRPKKVVYVSCDPATQARDIRYLADRGFSLQILRPFDMFPQTKHIE
                     VV"
BASE COUNT         2343 a         3018 c         2894 g         2183 t
ORIGIN      
        1 gcccgagttt cctcgtgatg atccggtcga tctccgtcgc tcggggatcc ctcagcgaat
       61 cttcgaaata aacaactcgg ccgtccctga cgaccctcgt ttcgatacag tcatggcggt
      121 cgaggtcgaa aggtctttgg taagcccgga tttcattttc tgcaccgaac cctctgacca
      181 tacggcaagc caaatgcctt ctatcgtcgc tgacaagaaa gagacaggat gccgaaaaac
      241 ccaggtcctt ttcggcactc atcagtatcg acctcagtat ctcgtcgagc aggatttgct
      301 gggtaaggag attggatatg cgaaaaaccg atgcaatgac tctgtttttt gtttccagtt
      361 ctttttgcag atgttccata tctggtactc tggaggggga tggactcagc cgctccgggt
      421 ctcgaagctt cacctgagat attgcatgtt cggcgtcaaa ttgccactgc cgcatttatc
      481 ccgacccgag ccacttatct aaccgttccc ggcgacgtcc ctttcccatt catgaacaga
      541 aaacaaagaa agtctcgccg ccggtggatt gcgaacgccc atgccttgct atcatgaaag
      601 agcaaaagta atgccatctt tcctcaaaaa aaggggcggc aaagcccggc tcctatgtca
      661 ttccgacatt gtggaatgac gggctttacg ctaaatcctc ctcataggtt tacggggcgg
      721 cggggacaga catgagggca cccggcttca ccgacccagg gttgaggcat cctgttgaaa
      781 acgcgaacgg cctgttcgca ttcttaacag gtactgatac ccttctcata ggatcacacg
      841 gcgctgcagg taccgcaaga ccttgcccgg caacctacct gtgtgctctc cctggtcgat
      901 cacgacctcg ccgttcacga tgacgtactc gatcccacgg ctgtagcgat gggggtcatc
      961 ccatgtcgaa aggtcgatga ccgtgtcggg attgaagata acgaggtcgg cataataacc
     1021 gttcataatc cgaccccgtc ccgcgaggcc gaatttttcg gccgtcaaag aagtcatctt
     1081 ttcgatcgcc ctgccgaggg gcatcagtct ttcatccctc acgtaacggc cgagcatgcg
     1141 cgggaacgta ccgtaggacc ttggatgggg cttcccgaat gcaagcttgc cgtaaggcgc
     1201 cagggcccag ccgtcggaac cgatcacgac caggggatgg agaatgatgc gtttgaacgt
     1261 ctcttcattg agggaaaaat tgatcattcc cacctggtcg tcctcctcga ttatgaggtc
     1321 gcggatgaag tcgtaggggt ctttccctgc ctcccgggat gcctggagga tacttttacc
     1381 ggggagatgg ctgatctttt gggtgatgac cgaggtgatc attatattat cccagctgcc
     1441 gatcttttcc tcctgggcct tgatgctctc cctcagcttc ttctccaaca ctgtgccttt
     1501 agagttcttc aggtattccg tgatctttcc ttcccgcgcc cagcggggga agaagacact
     1561 cagaaaggtc gaggtggcgg tataggggta gcggtccgcc atgatgttga acccttccgc
     1621 cttgacctcg gatatccggg agagtacggc atggatctta gaccagtttc gcgggtacgc
     1681 cagtttcaag tgggatatct ggaggctcac ccgcgcctct gtggcgacct tgatcgtctc
     1741 ttcgacggcc tccatgaggt agtctccttc gtccctcatg tgtgtcgaat aaacgcctcc
     1801 gtaacgcgcc actacccggc agaggtcgat tatctcctcc tgctgggcga agacccccgg
     1861 cgtgtagatc aagccggtgg agagtccgag ggcgcccgcc tctatacagt cttccacgat
     1921 cgacttcatc tcctcgacct catggtcttc ggagacacgg tcctcgcagc cgacgacggc
     1981 ctcccgcagc gttccgtggc cgaggagggt cgcatagttg agcgctattc cgccctgctc
     2041 caggcgtcgg aagaatcccc tgatatcccg ccaatcgagg tcgaggtcgt acttctcctt
     2101 aagaatccgc cccctctgct ggaaggattc gtcgttcaag ggaaagaagg taaacccaca
     2161 gtttccgccg atttccgtcg tgacgccctg ccggatcttg ctctccgccc gtgggttgac
     2221 cagaagctcc acatccgtat ggctgtgggg gtcgatgaac cccggcgcca cgacgagacc
     2281 ggaggcatcg atcacccgtc cggcatgacg cccctccaga cccggcccga gatggacaat
     2341 ccttcctcct ttcaccgcca catccacgtc ctgaccgggc gtaccaaggc cgttgtaaac
     2401 atgccctccc cggatcagga ggtcgtattc tttttcgttc tgcccattgc ttatcatttt
     2461 tcaccatcgt cttctgttgt ctaaaggctg cccgcccgaa tgtacggccg tcatacgagc
     2521 gcccataaga cggcgtacac cagtgagggc aggaggtttc ccacagggat ttttttcaac
     2581 tccaggatgt tgatcccgat agcgaggatg agcaccccac ccgtggcagt gacggcgctg
     2641 aggacgcggg ggtcctgaag aaagagaagc tgggaggcca aaagggtgat cgtaccctgc
     2701 actactagga cggggatcgc agaaaacgcg acgccgaggc cgagggtgga agcaaaggca
     2761 acggaggcta cgccgtccaa catcgccttg agatagaggg tgcttgcgtc accgaccgca
     2821 ccgtcttgga tggcgccgac gatcaccata gccccggaga tgtacaggac cgatgccgaa
     2881 acaaatcctt caacaaaggt tgcggaaccg gaatggaacc gctctttcag ccattccccc
     2941 agtttctcca cccaatcctc gacttccagg agttcccctg ttaatgcccc gaggagaagg
     3001 caaccgacga cagggatgac ctctcttacc gatagggcca tctggagacc aataaccaca
     3061 acggaaaggc cgagggtctg catgacggtc tttttgaacc tctccggcag acggttaccg
     3121 accgagaggc cgatcaggct cccgacgatt atgccgccgc tgtttacaag cgtccccttc
     3181 aagaaacctc ctctctcctt aatcgcgacc gacgttgccg cacgtcttcg tatttctata
     3241 ccaccgccgt tgaagggtga caagaatcaa aagcggacct tccaccggta ttgacatcga
     3301 cggggaaaaa tgcaagaaac aaccccatga aaacgcacac ttttcttttt cgggagggac
     3361 tatggtcggt agaaggtgtt tatttcgatg accgcggccg tcccatcgcc ctcgagggta
     3421 agacacgggt cacacaccta cccaggctgt gggtgaacga aggcgccatg gagctgaaat
     3481 ctggggaagt ccccattgtc gtccataacc ggtatgaagt cacccccttc gaagagggcc
     3541 gctccacgac ctcgtggaag tcttcgaacc ctgcggtcgg ggagttgaac gggagtttca
     3601 tcctcgttga tgatgcgatt ctctcagtct gccggtcagg tgacggtctg ttcggcggct
     3661 ctgaattcat gctccgggtc aacgacgaac agtacctcag ccgtggcgca ttccttagag
     3721 aaaacgaaag gctgtcctcc tggtccgtca cgctgcgaaa gatcaaggac ggtccctttc
     3781 ccgaatcggt gtgaaacggg ggaggaaacg aaggaccaaa aaaaagggcc ccgttttgga
     3841 ctcgccgggg ccctttcgtg agttttaagg tgcgtcggtc atccgacgac ggatttgatg
     3901 agctccacca ccggtgacgt gaagagggcg atgaggacag tgtacagggc gaacgcaccc
     3961 atgacttcgc tcatgtagag cttgttatac ttgtgccgca gggagatgag gataagcccg
     4021 ccgactatca gggcaccgag atggaagtaa gggaagttgc tagcgccggt cgcgctgtac
     4081 tcagcgatcg cgaacgtgcc cgttaccaaa actacgaaaa ccgccatact cagagtggaa
     4141 agtacctttt tcatctttat ttcctccttt ttttattgtt tttttgattc aatcggtgtc
     4201 tgtttccttt ttacttcatt atatgaaatt atcatgccag attatctctc gcttcagcat
     4261 atttcgtaac taattgataa ataaagatat ttaagtaatg cccctcctcc gtcctcctcc
     4321 ccggagagaa aaatcacccc tcttttgtaa agattcgttc aacgacattg aatgaaaggt
     4381 tcaaggtgtg gtaatgaaca gcatcatgga gaaagtcgct ccgggtattt ttctggtccc
     4441 cggagaaaac agatcgctgt tcccgttctg cacctgcctt tacttgaagg gcagaaactt
     4501 gcgggtcctg atagatgcgg gcatgggcga cgcgcggatg gacgcctgcc ttcgtgaagg
     4561 gatcgatgtc ctgatcctga cccactgtca tttcgaccac caatcgacca tcgggcgcgt
     4621 tcgtggcatc ccggtctggt gccacgatgt agaagtcccc tacctcgagg acagggatgt
     4681 ttacctccgg gaaaccggca tactcaggag cggcatagac atcgaccgct ttttcaagag
     4741 acatcccttc ccggacctgc gcgtagatag gcatctcacc gacggagaaa gaatagacct
     4801 gggcggcctg accctccagg tggtccacgc cccgggacat acgccgggcc actgtgcctt
     4861 ttacttcccc gaggcgaatt tcctgttctc ggcagacgtg gcgctcaaca ccttcggccc
     4921 cttttacggt aacgacttcg gcagcatcga ggactttatc gcctctatac gcaagctgaa
     4981 ggcgctcggc gcctcgacgg tggccacgag ccattcggga cctttccgca acaggctggc
     5041 ggagcgtttc tctcaatacg agaacatcat ctacaggcga gacgagctga tccttaagta
     5101 cctatcgggg aataaaacgc tcgcggggtt tctcggcaaa cacgttgtgt acaaagatta
     5161 ccccgaaccc agggaggtca cgatgtggat ggagttggtc caggtcgaga agcaccttca
     5221 aaggctaatg gagaagggcc gggtcagaca agaaggagac ctctacatca aaacctgaaa
     5281 agtaatcccg caaggcaggg accggaacaa cacgtccatt aataaacccc ctcatgaccc
     5341 cccgccattc tcggttcact tttcagtgcc gtttaccccg cctctgacaa acataagcat
     5401 cgcggcagca accactgtga gggcaaggga aaagtagaag ggggccaata cgcctatact
     5461 ctcccagagc aaacccgcaa tcaccgaggc ggggagcgca cagagcccaa cgaccatctg
     5521 aaaccccccg aggccgctgg cccggtatgc ttcgggcgag agctccgcta cgaaggccct
     5581 ctggaccgtg tcgatggccc cacggtggag gccgtaaagg atgaagttgg cgacgaccat
     5641 aagatggctt tttacgaaca tgaagcccaa acagaccagc ccccatagga tgaaggcgat
     5701 caccatcaca gcctttcttc ctatccggtc ggaaagcctg ccgaacggca gggatgtggc
     5761 cgccgcaacc gctgtgaagg cgagatagag aacggggacg aaagtcactt cgaacccgaa
     5821 ctcctttgcg cagatcagga gaaaagaata gctgaaggaa ccaagggcga aaagggcgct
     5881 caggaggagg aagaaccgga aattgcggtc caggtctctc agccttatac ccttgaacac
     5941 cttcgagcca tccggcttct tttccctgcc gacggaaatg atcagtgccg cgccgatgaa
     6001 agacggaacg gccgcgatca agaacaggta ccgataacca aggaacccta cgaacaagat
     6061 gcaggtgagg gtgccgcaga cggctcccag gttgtccatc gtcctcaaga ggccgaagtt
     6121 ctttccccgg tcctcgtccg tagaaaggtc ggcgatgatg gcatcccgcg gcgccccgcg
     6181 tatctttccg gcccggtcga ggatacggaa ggggatcaga tgctgccagg ccgtcgagaa
     6241 ggcatagccc agccgggaaa gcccgccgaa aaggtaaccc agccagacaa agaccttcct
     6301 cttactgatc cggtctgaaa tgtagcccga ggccgcctgc gacagggaga cgatggcgtc
     6361 tcccaggccg tcgagaaaac cgaggacggc catgttggct cccagggacg tgacgaagag
     6421 gggccagacc ggatagatca tatccgaccc catgtcgttc aggaacgacg cccaggaata
     6481 ggtgcggatc gtctttttcg attctttctg gtccacaagg atcagccatc cccgctctat
     6541 cggactgtct taatttttct ggagattcgc accgattttt tcttgaatca gcttggaagc
     6601 aatgtcgtta cccgccaagc ccacccggac cccgacggaa gtggtgtctt tcgagacgta
     6661 ttccagggcg atatggacct tatcgtcacc gatcgtcgaa tcgatggtcc cggtgccgat
     6721 cttctttttt ttcgtcgttt ctccggcctt catatcggcg agcgccttct cgcaggccga
     6781 ccaagtttct tcgaaggacg ccttgtagtt cgtccaaagg acgccgtcgc tgtaaaagaa
     6841 tctgcccgat tcgatgccga cgaccttgtc tcccaccata acggcgcagc ccgaaagaat
     6901 catcgcgccc agagccagca aaaagaccat ggcgcatcgt gatggaccat tcatgaaagt
     6961 tcctccctga gtcagtgaaa taaagaaatc aaccggaaaa agtcccggca agatataccg
     7021 ccatgaaaaa gaagtcaacg ctgctgaagt tccgcgaggg taggtcagta aaagcgaaaa
     7081 tagatcgcct gcaggacggc ccacaggagc acgaggcatg tcaagacgat tagcccggcg
     7141 gccaggatct tgtggagctg attctccgcc cttttctcct catagccgac aaaaaacccc
     7201 gtcagtgccc ccgcgacgat ccctccgccg tgggcccagt tgttgattcc gggaacgagg
     7261 agcccgaaga gaatcagtcc gatgacccac ccgagggcct gcttgtagac ggcctgcccg
     7321 taaaacccgc cccggctctt gccgtaatag aggatggcgc cgatcagccc gcagaggctc
     7381 gccgaggcgc cgatggtgaa ggggatgccc gccaggaccg agacgtagaa ccccacgaca
     7441 ccgctgagga catagacgat gataaaacgg ccggtcccgt attcatgcat cacaaaaggg
     7501 ccgagctgac gcagggcggc catgttgaag aaaatgtgga gaatgctgcc gtggagaaaa
     7561 gaggcggaga cgagcgtcca ccaccggtga aaaagcccga ccgggatcgt gcccgtggcc
     7621 ccgaggacga agaggctgtt ttccgacggg gacaggaacg tgaaggggtt cgccgaaacg
     7681 ccgatcttcc gtgggtccag gacgatagac aggatgtaaa aggcgatgtt ggcgtaaaaa
     7741 acgacagaaa cgatgtcccg agagccgatg gaatcggcca ggtatctctt ccacgacgcg
     7801 cccggaccga caatcccgca ataggggcat ctttcttcgt cgtgattgat gagcttccga
     7861 cagttcgggc agatgatggg cttcttttct ttgttcggca tgagtctccc ctgggtgtga
     7921 aatgtcagcg tctcagcgca aaaaatgcat catggccggg atggccacca aggttcccgc
     7981 tgaagccgtc aggttgacaa agaccacaag aagcaggata cgggtgatct tgttccgcca
     8041 gaaaccgcgc accgtcgtga tgtcctcggc gaggtcgagg aagtccttca cctggggctt
     8101 cctcagagac gcctcggcga gtcccgcgat ccagcctgtc gcgattaaag gatggatcgt
     8161 ggcgacgggc gccgagatcg cggccgtgac aatcgtgagc ggatgggcga gaacggccag
     8221 ggcgccgaca gccgcggaca gggccgtgat cgccacccag gatttgacca tgttgaaact
     8281 ggcctggctg ccggcgtaga agaacccacc gatgatgaga cccaggacac cggcgaggag
     8341 aacccactgg acgacgtcgc cccacgggcc cctgggagga accttgttca gggcctcgat
     8401 gtcggcctcc ttgccctcat tcttcagaat ccccggtata tgccccgcgc cgactactgc
     8461 cacgaccttc cttcccggcg cgtgtcggat agtatgggaa aggtactcgt ccctttcatc
     8521 tatcagggtt gttttaatct cgggcagttt ctgaccgaag aggttcaggg cgacctcaag
     8581 ggcatcacgc tttttgagct tctcgatctc ctcctcgctg atctcttccg ccatgatcag
     8641 cgacgccacg gcctcgggca acagcttgaa tttactccaa catcccatct ttcgccaggt
     8701 gcgcatgagg gtcacgcgga tgtcccggtc tgcgggtacg acctccgccc cgattttctc
     8761 ggcggcgacg acggccctgt gcatctcctc gcccggcctg atgtcgaact tttccgccat
     8821 ccgtttttgg aacgacgcca taagcaattg ggacagcaga accgaagtcc gtttttccct
     8881 gatgactttg acgatgtcgg tctcgagcca cttgcgtgga ttcttgagcg cctccaacct
     8941 cgtcttgcac agctcgacgc agacggtgtc cggtttctcc ccttcgatca ccgcctcgac
     9001 caggtcggca ctttccttgg agacgtgagc ggttccgacg agcaccacct ctttgccgtt
     9061 aaagctacgg cggtgaatat ccgcgtgttc caatcctttt ccctatcctt tactcacact
     9121 tttttgttcg gtgcgcgatt tcccttgtgg acattggaag ctatcagtat tttatcccgt
     9181 tgtaaagggt ggtggaagga cggaaaggga agtggtataa gccaaaagca gtcaaaaatc
     9241 tttcctttaa tcattcgatg cctgacgtga tggatgcaag attcagaatc ggtcaaacgg
     9301 tagaggctat gatcgagacg gtcgccttcg gcggcgacgg ggtcgccagg gtgcgcgaca
     9361 tggtcctctt cgtccccttt accgccgacg gggacctggc ggaggtcagg gtcgtcgagg
     9421 tgaggaaaaa tttcctccgc ggaaagctga ggagattact catcccatca ccccatcgga
     9481 cagagcctga gtgcccttat tatcttcggt gcggcggctg ccggtaccag cacatatcct
     9541 acgagcatca gctcgagctg aaaagaaatc aggtcgccga gtcgttcaag aggatcggcc
     9601 gattctcctc gccccctgtg gaagacatcc gtccctcgcc gaagagttac aactaccggg
     9661 gaaaagtgga aatccacctc aggactaaga ggggttaccc ccccgaaatc gggttcatcg
     9721 acacaggagg gctgaggata gtcgacgtag agcgttgtgc gatcgcggat gagacgatca
     9781 acaacgccct ccgcgaactc agggggaagg cgctctccgg agacctgccc atcggagggg
     9841 aggagacaaa gatactctgg tcgggagacc aggatatgaa tgaagacggc ccgatcaccc
     9901 gggtcgtcaa aggccgcaaa cttgaagtcc accggcaggg ctttttccag gcaaacctgt
     9961 ccctcacgga cgcgctcgtc gatgccgttc ttgacctggc cgccctgacg ggcggcgaga
    10021 gagtcctcga ctgctactgc ggggtcgggc ttttttcgct ctttctcgcg gcaaaggccg
    10081 gggatatcat tgctgtcgac atcgaccggg aagccgtcgg cgcggcacgg aaaaacttcg
    10141 ccgccttcgg acatgggggc gtccgcctgt ttgaggggtc cgtcgaagac attctcaggg
    10201 aacatctagc cggagagaaa ggcaggacgg acgtcgtcgt gctcgacccc ccgaggacgg
    10261 gttgcgacaa ggttctcctg gatactctgg cggaatggag acccaagaag gttgtctacg
    10321 tctcctgcga cccggcgaca caggcacgag acatccggta cctcgccgac cgggggtttt
    10381 ctttgcagat attgagaccc tttgatatgt ttccccagac gaaacacata gaggttgt
//
LOCUS       DAOTMX010000015        10408 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_5995_length_10408_cov_3.801893, whole genome shotgun sequence.
ACCESSION   DAOTMX010000015 DAOTMX010000000
VERSION     DAOTMX010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 10408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10408
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5995_length_10408_cov_3.801893"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="P5573_00900"
     CDS             complement(<1..842)
                     /locus_tag="P5573_00900"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HRT26657.1"
                     /translation="MSFRRADLSGVNIVKTGFKECDFSLARFDDTDLSKVNMSSMKFK
                     KASFRKARFFQTILNGADMTGADMTEAIVSWSLFQDALLDGATFHGAKVEKNSFLHTS
                     LKKTDFTDTDLVKAAFLEADVTGAIFRGARLFKVLLQNCNLAEQNFRGMSIQQVNFSG
                     SDLKNSDFTGADISMSNFMNADLTGATLAGVKSRDAMFMGAKLIKANLIGSDLAQAYF
                     GEADLSQADLSGAILYNAVFEKAKCRASRFHKCDCGYADFSYTDLTAADFKDASLFMA
                     RMHST"
     gene            complement(953..4711)
                     /locus_tag="P5573_00905"
     CDS             complement(953..4711)
                     /locus_tag="P5573_00905"
                     /inference="COORDINATES: protein motif:HMM:NF021456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2169 domain-containing protein"
                     /protein_id="HRT26658.1"
                     /translation="MKIYKKNELSLLIKSFGIADRLYIAVSVLIFFDLNDPDTSLKEQ
                     ELWSVIPAQLGQGAVFDPGMAKPRGEVLVTGSCFAPRGLTRRASQVSLRVGNLYKTLN
                     IFGNRFWKKTGGILKEISPAEPFTEMPLIWQNAFGGKGFERNPLGKGIDPTPSPDGTL
                     RVYLPNIEHANLLVGSPADRPDPAGFAPLDMMWPQRMKKTGTYDDKWLREHWPNFPQD
                     MNYEYFNAASEDQFIEGFFRAGESIEIRNMHPDLPLIQSRIPCIRPRCFVTKKTSLKA
                     TRESDSIFQEVTTRIDTVWLFPSVLRGVVIHRGTTEILDEEYADVQRIFLDIERMDEA
                     PKPIEFYLEEQKKVFSRAVPIDPAPLEAAKKKIRDAMLLVKNLPKEIERAKAEILGKT
                     PVMPQTPEETVTRAKMVLTESAMLLNRMEGMTKDMQARYSHVVNINKVDFDGIRNKLS
                     GVEGHLDKIMAEVQQAEEKATDAQKELSGHLKERVPPDVLAKAGVDPDHPTAVAGSVD
                     TWHDRGFPFVVQCRKNLESHPGMQSLFHRVGFTRKTVERAWFGVHPAETTNERTEWGL
                     KEKKDARGNPLPLIIPPGIVIPRFHEAYLNGINICRTDFREGIYPQEAVLIEGSEEAP
                     RVLPAAAADDRTPWIVVTTELEALLMEQEVGDACSIIALKDAGDKPPKDAEKAFKDAP
                     VVLVVFPEHGMSEAEVQKWRSIYPNSRGIALPKGKDILDAGRKKVDLRQWIMGALPPN
                     FTREHAVEPELPEPGKPVEKKPFSGLSLSLPDIRALAASLTEETKAALQPQVDAGNAI
                     QKEMMNEVKVILGNLNMDAENLLSPSQPQKSITQMAREMADQIAAQREKIRDQGVLTR
                     EAEVKMDAAARKISQMGKEAEIRYQEGMAKLAAAQKEIDIAKSGGIPDSVKAQFKGTG
                     IDPEKIRRLTREEVIELYGKGESLSGAILSEVDLSDLDLHGIDLSTAQCQKTKFCNTN
                     LEEANLFQILAMEADFSAARLRGAKAYRGIFTKALFKKADLRTAEFRSAVLQEADMTE
                     ANMQDVKLNMSILQKAKLVKAQLSGVDANMCILSEVEATQARFLNGNFKKCVFKNACL
                     DDTDFSNATLNSTIFFGAKGKHVTFAGADMDKARMGGQAAFPEADFRNIKMKHGCLRE
                     ANLAGSSFKGAVIHESIIEDCDLNQADLRRISAKHTRFTKTNLEGARMQEMNLFQGSL
                     RKARLVNANLQGANLFGVDLFKATVGKTDVTGANLKMTQLDKREEYLK"
     gene            complement(4715..>5313)
                     /locus_tag="P5573_00910"
     CDS             complement(4715..>5313)
                     /locus_tag="P5573_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT26659.1"
                     /translation="TENKIILGEETETVVVGYSHNVLGASWETVVGLDMEFKVGGSRE
                     FKTYQHEFRGQKVRLGAETLDLASNSVEIQGTVRHVRGSKTEVRGNATTVQGSHTTVK
                     GNATNVTADNTVVRGNADEVRGERTMIEGSVTRITGDNTAVTGNATNVTGNNAVVSGE
                     STTVSGGTVTVSGNNTNVSGDVATVNASRVTIGTQVFM"
     assembly_gap    5314..5413
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5414..6251)
                     /gene="vgrG"
                     /locus_tag="P5573_00915"
     CDS             complement(<5414..6251)
                     /gene="vgrG"
                     /locus_tag="P5573_00915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HRT26660.1"
                     /translation="MEYLTSKKFSFVSVAFSADTFGVISFKGSEAISQPYGFEILLVS
                     VELTIDHETVIQNPARLIIHRDDGDDVLYQGILLSFEQLHNFGGVVFYRAHLVPMLYW
                     LSLTHHNQVILDKSLPNFLMDVLKDGGLTSLDFEFRLQETYLPVDFVCQYGESHLNFV
                     SRWLEREGIYYFFEQTQSGEKVIFTDSRMTHTSMPQGKSVRYSPPSGLEALHLQEIIS
                     RFTCRYSLTPSTVIVSDYNDIKPSLDVTGKAEIDADGRGRFYSYGDHFLTPEEGQRMA
                     RIR"
     gene            complement(6254..6949)
                     /locus_tag="P5573_00920"
     CDS             complement(6254..6949)
                     /locus_tag="P5573_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT26661.1"
                     /translation="MKILKVSFTILARAPFRPTPDTSSPEMISVAMETLYDAVAACRP
                     QLCIPQPADICPAGGVTVCPTRMKNMTPDGLIRITPYLKDLHDADEFIEQSTASGKTP
                     EEAARYLKTAWPVPPLDLSLPARETRARPRNAVDNILSMMAVQGQPAAEPAGPKTWKK
                     QINDMLARLLKNIFDSDVFRGFEAAWRGVEGILMYGRPHPLSDYPSHGGWNLLFYGSN
                     VAAKSPFCTLNVD"
     gene            complement(6977..9601)
                     /gene="tssH"
                     /locus_tag="P5573_00925"
     CDS             complement(6977..9601)
                     /gene="tssH"
                     /locus_tag="P5573_00925"
                     /inference="COORDINATES: protein motif:HMM:TIGR03345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HRT26662.1"
                     /translation="MVTVDIKSLLLHLNSFCTNALQNAAGLCVSRTHYEITVEHLFTK
                     LLEDPDSDLPLIFRQFDLDPGRVQKALDETLEEFKTGNAARPVFSPLLMELLQDAWLI
                     ASIDLGEKNIRSGAILLSFLGKPAFFAMGRYVDLIKKIGRETLVNEFWAMTKSSVEKT
                     AAVREPGAAEPVPGKADGGFLARFCTDFTERARAGGIDPVFGRDNEIRQMIDILARRR
                     KNNPICVGEPGVGKTAVVEGMALRIVEGDVPDLLRDVTLLGLDMGLLEAGAGVKGEFE
                     TRLKGVINEIKASEKPIILFIDEAHTLIGAGGTAGGSDAANLLKPALARGELRTVAAT
                     TWTEYKKYFEKDPAMARRFQLVKLDEPSVDTTKVMLRGLKASYEGAHKVIVRDDAVES
                     AVNLSDRYITGRFLPDKAIDLLDTSCARVKINLTAKPTALEDKERTFQALEREIKALD
                     RDRVGGVRIDKEKYLSVVEKKEKTSAEADEIRERWLKEKEAAQKVIEIREERYRVTES
                     ASDDREGLEARLAEAERTLADIQGTSPLIQTEVNPDVVAQVVSDWTGIPLGKVLKDEA
                     QTIINLDERMKERIKGQDHALSSMAEVIRAAKSGIKNPNQPIGVFLLVGPSGTGKTES
                     GLVLADLLFGSERHVVTINMSEFQEAHTLSRLIGSPPGYVGYGEGGMLTEAVRQKPYS
                     VVLLDEVEKANIDVMNLFYQVFDKGMLTDGEGKEINFKNTILILTSNLASDIIQEMTA
                     MGEAPPLEAVLGAVRPILSKHFKPALLARMTVIPFYSLNTEAMRLIVELKLKKIQKML
                     MENNRMAMTYAPAVVDRINERCTEVETGARNIEYILNGNVLPRLSRTILSHITEGGLP
                     MSVHLDVDETGAFTFAFS"
     gene            complement(9714..>10408)
                     /gene="tssG"
                     /locus_tag="P5573_00930"
     CDS             complement(9714..>10408)
                     /gene="tssG"
                     /locus_tag="P5573_00930"
                     /inference="COORDINATES: protein motif:HMM:TIGR03347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HRT26663.1"
                     /translation="LLTHDSRDFIDIFNARLYSLYFRCWSKYRLFFNLVEEIDQEVLE
                     RLYCLIGLGGKKLRESVENPHEKIRYAGLMTQLPRCAEGLKSLLADVLHDPAIQIIQC
                     VERTVEIPEEQRFILGVSGNLLGEDAYVGSDTYERLGKFRVCIGPLTGEDFNRYLPDK
                     PIFHRLKSFIGFYLDQPLAWDVEAVLIPGEAATTKFGDEKRCRLGWNTWLFSDGFQPA
                     DTAVRFTDQGLH"
BASE COUNT         2056 a         2989 c         2718 g         2545 t
ORIGIN      
        1 gtgctgtgca tccgggccat gaataggctc gcatccttga aatcggccgc cgtcaggtcc
       61 gtgtaggaga aatcggcata accgcagtcg catttgtgaa accgggaggc gcggcacttc
      121 gccttttcaa aaacggcgtt atacaggatc gctccggaga gatcggcctg ggaaaggtcc
      181 gcctcaccga aataggcctg ggcgaggtcg cttccgatca ggttggcttt gatgagcttc
      241 gcccccatga acatcgcgtc ccggctcttg acccccgcaa gggttgcgcc cgtgaggtcc
      301 gcgttcatga agttggacat ggaaatatcc gcccctgtga agtcggagtt tttcaggtcg
      361 gaaccggaaa aattgacctg ctgaatggac atcccccgga aattctgttc cgcaaggttg
      421 cagttctgca agagcacctt gaacagccgg gcgccccgga agatcgcgcc ggtcacgtcc
      481 gcttcgagga aagcagcctt gaccaggtcg gtatctgtga aatctgtttt ttttagcgag
      541 gtgtggagaa agctgttctt ttcgaccttt gcgccatgga atgttgctcc gtcgagaagg
      601 gcatcctgaa aaagcgacca actgacgatg gcctccgtca tatccgcccc ggtcatatcc
      661 gccccgttga ggatggtctg gaagaatcgc gccttgcgga atgaggcctt cttgaatttc
      721 atgctgctca tattgacctt cgacagatcc gtgtcgtcga aacgggcgag ggaaaagtca
      781 cattctttaa acccggtctt gacgatgttc acacccgaca gatcggctct ccggaaggac
      841 acgaactcga agcggccccc tgtaagatcc gcgcccgaca agtcaatctc tgagaggtcc
      901 gtattttcta tccaacggtg ttgtcgtatg ctttccagca cttcctgtct cgtcatttta
      961 gatactcttc ccttttgtcg agctgggtca tctttaggtt tgctcccgtg acgtcggtct
     1021 ttcccaccgt tgctttgaat aaatccactc cgaaaaggtt agccccctga agattcgcat
     1081 ttaccagcct ggccttcctc aaagagccct gaaagagatt catttcctgc attctggctc
     1141 cttcgaggtt cgttttagtg aatctcgtgt gctttgccga gatgcgccgg agatcggcct
     1201 gattgagatc gcagtcttca atgatggatt catgaatgac cgcccccttg aaggatgagc
     1261 ccgcaagatt tgcctctctt agacacccgt gtttcatttt gatattacga aaatccgcct
     1321 ccggaaaggc ggcctgtccg cccatccggg ccttgtccat gtcggcgccc gcaaaagtca
     1381 cgtgttttcc tttcgcgccg aagaagattg tcgaattcag ggtggcgttg gagaaatccg
     1441 tgtcgtccag gcaggcgttc ttaaaaacgc attttttgaa gttgccgttg aggaaacggg
     1501 cctgcgtggc ttccacctcg gatagaatgc acatattggc atccaccccg gaaagctgcg
     1561 cttttacaag tttcgccttc tgcagaatgg acatattcaa cttaacgtcc tgcatgtttg
     1621 cctcggtcat gtccgcctcc tgtaagacgg cggaacggaa ttcggcggtt cgcagatcgg
     1681 cctttttaaa gagcgccttg gtaaatatcc cccgatacgc cttcgcccct ctgagacggg
     1741 cagctgagaa atcggcctcc atggcgagta tctggaagag attcgcttcc tcgaggttcg
     1801 tattgcagaa ttttgttttt tggcactggg cggtggagag gtcgatgccg tgcaggtcga
     1861 ggtcggatag atccacctcg gaaagaatcg ccccggacag ggattcaccc tttccgtaca
     1921 gttctataac ttcttcccgt gtgagcctcc gtatcttttc cggatcgatg ccggtgcctt
     1981 tgaactgggc cttcaccgag tcgggaatgc cgcccgattt tgcaatatcg atttctttct
     2041 gcgccgcggc aagtttcgcc atgccttcct ggtagcggat ttccgcctct tttcccatct
     2101 ggctgatctt tctggctgcg gcatccatct tgacttccgc ttctctagtc aacacgcctt
     2161 gatctctgat cttttcccgc tgggcggcga tctggtcagc catttccctt gccatttgcg
     2221 tgatcgactt ttgcggctgc gacggagaca gcaaattttc cgcgtccatg ttgagattgc
     2281 cgaggattac ctttacttcg ttcatcattt ctttctggat ggcgttcccc gcatcaacct
     2341 gcggctgcaa ggccgcctta gtttcctctg tcagtgaggc cgccagggcc cggatgtcgg
     2401 gaagcgaaag gctgagtccc gagaaaggct tcttctccac gggttttccg ggctccggaa
     2461 gttccggttc gactgcgtgc tcccgggtaa aattcggcgg cagcgccccc atgatccatt
     2521 gacggaggtc gaccttcttc cgccctgcat cgaggatgtc tttccccttc gggagcgcga
     2581 tcccccggga gttgggatag atagaccgcc acttctgcac ttccgcttca gacatcccgt
     2641 gttccggaaa aaccaccagg accacaggag cgtccttgaa ggccttctcc gcgtccttgg
     2701 gaggcttatc cccagcatcc ttcagcgcga tgatcgaaca ggcgtcgcct acctcctgct
     2761 ccatcaggag cgcctccagc tcagtcgtga cgacaatcca cggcgtacga tcgtccgccg
     2821 cagccgcagg aagcacgcgg ggtgcctctt cggaaccttc gatcaggacc gcctcctgcg
     2881 gatagatgcc ctcgcgaaaa tctgttctgc agatatttat cccattgaga tacgcctcgt
     2941 gaaaccgtgg gatcactatg ccagggggta ttatcagcgg cagggggttt ccccgggcat
     3001 cctttttttc cttcaaaccc cattctgtcc tctcattcgt cgtctccgcc ggatggacgc
     3061 cgaaccacgc acgttcaacg gtttttcggg tgaatcccac gcggtgaaag agggactgca
     3121 tgcccgggtg ggattccaga ttcttgcggc actgcacgac aaacggaaaa ccccggtcat
     3181 gccaggtatc aacggacccg gcgaccgctg taggatggtc gggatcgacg ccggctttag
     3241 ccagaacatc gggtggcacc cgttctttga gatgaccgga cagttctttc tgggcgtccg
     3301 tcgccttttc ctcggcctgt tgtacttccg ccatgatctt atccagatgc ccttccaccc
     3361 ccgacagctt gttccggatg ccgtcaaagt cgaccttgtt gatattgacg acgtggctgt
     3421 aacgcgcctg catgtccttc gtcatgccct ccattctgtt caagagcatg gcgctctccg
     3481 tcaggaccat ctttgctcgg gtcaccgtct cttccggcgt ctgcggcatg acgggcgtct
     3541 tcccgagaat ttcggccttg gccctctcga tctctttggg aagattcttt actaacagca
     3601 tggcatcgcg gatcttcttc ttggccgctt ccagcggcgc cgggtcgatg ggaaccgccc
     3661 gactgaagac cttcttctgt tcttccaggt agaattcgat gggtttgggc gcctcatcca
     3721 tcctttcgat gtcaaggaag atacgctgca catcggcata ctcttcgtcg agaatctccg
     3781 tagttccccg gtggatgacg acccctcgaa ggactgaagg aaacagccag accgtatcga
     3841 tgcgtgtggt cacttcctga aagatgctgt cgctttcccg ggtcgctttc aggctcgttt
     3901 ttttcgtcac aaagcagcgg ggacggatgc acgggatacg ggattgtata agcggcaggt
     3961 cggggtgcat gttccgaatt tcgatggact cgccggcacg gaagaagcct tcgataaact
     4021 gatcttccga agctgcgtta aaatactcgt agttcatgtc ctgcgggaaa ttgggccagt
     4081 gctcccggag ccacttgtcg tcataagtcc ccgtcttttt catccgctgt ggccacatca
     4141 tatccagggg agcgaatccc gccgggtcgg gacggtcggc cggcgaaccg acgaggaggt
     4201 tggcatgttc gatattgggc agatagaccc ttagtgttcc gtcgggcgac ggcgtcgggt
     4261 cgatgccttt ccccaggggg tttctctcga atcctttgcc gccgaaggcg ttctgccaga
     4321 tgagcggcat ctccgtgaac ggttccgccg ggctgatttc tttaaggatg ccccctgttt
     4381 ttttccagaa gcgatttccg aagatgttca aggtcttgta taggtttcca acccggaggg
     4441 agacctgcga cgcccggcgc gtcagtccgc ggggagcaaa acaagatccg gtgaccagta
     4501 cttccccccg cggttttgcc atacccgggt cgaatacggc gccctggccg agctgggccg
     4561 ggatgaccga ccacagctcc tgctccttga gggatgtgtc gggatcattg aggtcgaaga
     4621 aaatcaggac ggacacggct atgtaaagcc tgtctgctat accgaaagac ttgatgagga
     4681 gcgacagctc gtttttttta taaattttca tgggtcacat aaaaacctga gttcctatcg
     4741 tgacgcgcga cgcgttcaca gtcgcgacat ctccgcttac attcgtgttg tttccactga
     4801 ccgttaccgt gccgccagat acggtcgtcg attcccccga aacgacggca ttattgcccg
     4861 ttacattcgt ggcgttgccc gtaaccgccg tattatcacc ggttatgcgt gtcaccgagc
     4921 cctcgatcat tgtgcgttcc cctctgactt cgtcggcatt cccccggaca actgtattgt
     4981 ccgctgtaac attggttgcg tttcctttga ctgttgtatg cgacccctgt acggttgtgg
     5041 catttcctcg aacctccgtt ttgctccccc taacgtgccg tacagttccc tgtatctcca
     5101 cgctgttact tgcaaggtcc agtgtttctg cacccaacct tactttctgg cccctgaact
     5161 catgttgata ggttttaaat tcacggcttc cgccgacctt aaactccatg tccagtccca
     5221 ccactgtttc ccagcttgcg cccagcacgt tgtgggaata tccgacgacg accgtttctg
     5281 tttcttcgcc cagaatgatc ttgttttcgg tttnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnncccgtat ccgcgccatg cgctgcccct cctccggcgt caaaaaatgg
     5461 tccccgtagg aataaaaccg gccacggcca tcagcatcga tctcggcctt cccggttaca
     5521 tcgagagacg gctttatatc gttgtaatca ctgacgatga cggtggaagg tgtcaggctg
     5581 tatcggcacg taaaacggct tatgatttcc tgcaggtgca gggcctccag gcctgaaggc
     5641 ggtgaataac gcacagactt cccctggggc atggacgtgt gcgtcatccg gctatctgta
     5701 aagatgacct tttcgccgct ttgggtctgc tcgaaaaagt aatagatccc ttcccgctcc
     5761 agccatcggg aaacgaagtt gaggtgactc tcaccgtact ggcagacaaa atcgacgggc
     5821 agatatgttt cctggagacg gaattcaaag tcgagggaag tcaaaccgcc gtccttgaga
     5881 acatccatta aaaagttcgg gagagacttg tccaggatga cctgattgtg atgtgtgagc
     5941 gacagccaat aaagcatagg aacgaggtgc gcccggtaaa agaccacgcc accgaaattg
     6001 tggagctgct cgaagctcag gagtatcccc tgatagagga catcatcccc atcatcgcgg
     6061 tggatgatca gtcgggcggg attctgaatg acggtctcat ggtcgatcgt cagctcgacg
     6121 gagaccagca aaatctcaaa tccatagggc tgcgatatcg cctcgctccc cttgaaacta
     6181 atcaccccga atgtatcggc cgagaacgcg acggatacga aagaaaattt ctttgaagtc
     6241 aagtattcca tggtcaatcc acgtttaatg tgcaaaaggg acttttagct gcaacgttgc
     6301 taccataaaa aagtaaattc cagcctccgt gagaggggta atcagaaagc ggatgaggcc
     6361 tcccgtacat aaggatgccc tccacgcccc gccacgcggc ctcgaagcca cggaaaacgt
     6421 cactgtcgaa tatgtttttc aggaggcggg caagcatatc gttgatttgc tttttccacg
     6481 tcttcgggcc ggccggctcg gccgccggct gcccttgtac ggccatcatg gagagaatgt
     6541 tgtccacggc gttccgcggg cgtgcccggg tttcccgtgc cggcagggac aggtcaagtg
     6601 gcggcacggg ccatgccgtc ttcaggtatc tcgcagcttc ttccggggtc ttcccgcttg
     6661 cagtcgactg ttcgatgaac tcatccgcat catggaggtc tttcaaatac ggcgttatcc
     6721 ggatcaagcc gtcaggggtc atgttcttca tccgggtggg acacacggtg acgccgccgg
     6781 cggggcagat atccgcgggc tgcggtatac ataattgcgg gcggcaagct gctacggcgt
     6841 cataaagcgt ttccatcgcc acggagatca tttcaggaga ggaggtgtcc ggtgtcgggc
     6901 ggaaaggtgc ccgggctaag atggtaaaag aaactttcag tattttcatg atacgccctg
     6961 agatactccc ttccaattat gaaaatgcga atgtaaacgc ccccgtttca tccacatcga
     7021 gatgcacact catcggcagg cctccttcgg tgatgtgcga aagtatcgtc cgggacaacc
     7081 ggggaaggac attcccgttc agtatgtact cgatgtttcg ggcgcccgtt tccacttccg
     7141 tgcatctttc gttgatccga tccaccaccg ccggtgcgta ggtcatggcc atgcggttgt
     7201 tttccatgag cattttctgg atttttttta gcttgagttc gacgataagt ctcatggctt
     7261 cggtattgag actgtaaaac gggatgacgg tcatgcgagc caagagggca ggtttgaaat
     7321 gtttcgacaa aatgggccgc acggccccga gaacggcttc caacggaggc gcttccccca
     7381 tggccgtcat ctcctggatg atgtcggagg cgaggttgct cgtcaaaatc aggatcgtgt
     7441 tcttgaaatt gatttccttt ccttccccgt ccgtgagcat gcccttgtca aagacctggt
     7501 agaagaggtt catgacgtcg atattggcct tctccacttc atccaggaga accaccgaat
     7561 aaggtttctg gcgcaccgcc tccgtgagca tgccgccctc gccgtagcct acatatccgg
     7621 gaggggagcc gatgagacgg ctcagcgtgt gggcttcttg aaattcactc atgttgattg
     7681 tgaccacgtg acgttcgctg ccgaaaagca aatcggccag caccaggccc gattcggtct
     7741 tgcccgtgcc gctcgggccg acgaggagaa atacgccgat tggctggttg ggatttttga
     7801 tccccgattt tgccgccctg attacttccg ccatgctcga aagagcgtga tcctgtcctt
     7861 tgatccgttc cttcatgcgc tcgtccaggt tgatgatcgt ctgcgcctcg tccttgagga
     7921 cctttcccag cggtatgcct gtccagtcgg aaacgacttg ggcaaccaca tcaggattga
     7981 cctctgtctg gatcagggga gaggttccct ggatgtccgc cagcgttcgc tccgcctccg
     8041 ccaaccgcgc ttccagtcct tccctatcgt ccgaggcact ctccgtgact cggtatcgct
     8101 cttcccgtat ctcgataact ttctgtgccg cctccttctc cttcagccag cgttccctta
     8161 tctcatctgc ttccgccgac gttttctcct tcttctccac gaccgacagg tatttctcct
     8221 tgtcgatcct cacgccgccg acgcggtccc ggtcgagcgc cttgatttcc cgttcaagag
     8281 cctgaaaggt cctttccttg tcctcaagcg ccgtcggctt tgcggtaaga ttgatcttga
     8341 cgcgggcgca gctcgtatcc agtaggtcaa tggccttgtc ggggagaaac cggccggtaa
     8401 tgtaccgatc ggagaggttt actgcagact ccacggcatc gtcccggacg atgaccttgt
     8461 gcgccccttc gtaactcgcc ttcaatccgc ggagcatgac cttcgtcgtg tcgacactcg
     8521 gctcgtcgag cttgaccagc tggaaacgcc gggccatagc ggggtctttc tcgaagtact
     8581 ttttgtattc cgtccaggtg gtcgccgcca ccgtccgcag ctcaccccgc gccagggccg
     8641 gcttcaggag gtttgcagcg tcgcttcccc ctgcggtccc gccggcgccg atcagcgtgt
     8701 gcgcttcgtc aataaaaaga atgatcggtt tttccgacgc ctttatttca ttgatgaccc
     8761 ccttcaggcg tgtctcgaac tctcccttta cgccggcgcc cgcttccaga agacccatat
     8821 cgagtcctag gagcgtcacg tccctgagca agtccggcac atcaccctcc acgatgcgca
     8881 gggccatacc ctccaccacg gcggtctttc ccacgccggg ttcgccgacg caaatggggt
     8941 tgttcttcct tcgcctcgca agaatgtcga tcatctggcg tatctcattg tctctgccga
     9001 agacagggtc gatcccaccg gcccgcgccc tttctgtgaa atccgtgcaa aagcgggcga
     9061 gaaaaccccc atctgccttt cccggaaccg gttctgccgc ccccggctcc cggacggcag
     9121 cggttttttc cacggacgat ttcgtcatgg cccagaactc gttcaccagg gtctcccgac
     9181 cgatcttttt gatcagatcc acatagcgcc ccatggcgaa aaacgcaggc ttccccaaga
     9241 atgacaggag aatggcccct gaacggatat tcttttcccc caggtcgatg gaggcaataa
     9301 gccacgcgtc ctggagaagt tccatgagaa gaggggaaaa taccggcctg gccgcattgc
     9361 ccgtcttgaa ttcttccaat gtttcatcca gcgccttctg cacccttccc gggtcgaggt
     9421 cgaactgacg gaagataagc ggcaggtccg aatcggggtc ttcgaggagt ttggtgaaca
     9481 ggtgctccac cgttatctcg taatgggtcc gagatacgca caggcctgcg gcgttctgca
     9541 gggcgttggt gcaaaagctg ttcagatgta aaagcaggga ctttatgtcg acggtaacca
     9601 tacgtacccc cttgttcaaa ttctaaataa gttcaaatct cgaaatctta agcgtttcgg
     9661 caattctcga ttcggatgct gttcgtaaat caggattcag cttttcgtat ttttcaatgc
     9721 agcccctgat ccgtgaagcg tacggcggtg tctgcgggct gaaaaccatc tgagaaaagc
     9781 cacgtattcc agccgagccg acaccgtttt tcatctccaa acttcgttgt cgctgcttca
     9841 cccgggatta aaaccgcttc cacatcccag gccagaggct ggtccagata aaatccgatg
     9901 aaagacttca atctatgaaa tatgggttta tcggggagat accggttgaa atcttctccg
     9961 gtcaaggggc cgatgcaaac cctgaatttc cccagacgtt cataggtgtc actccccacg
    10021 taggcatctt ctcccagaag attccccgac actcccagga tgaatcgctg ctcctcggga
    10081 atttcaacgg ttctttccac gcactggatg atttggatag ccgggtcatg aaggacgtcg
    10141 gccaaaagcg acttcaatcc ctcggcacat cggggcaact gtgtcatcag gcccgcatac
    10201 cgaatctttt catgcggatt ctcaacgctc tcccgcagtt ttttcccgcc gaggccgatg
    10261 agacagtaca gcctctcaag aacctcctga tcgatttctt caaccagatt gaaaaataac
    10321 cgatacttgc tccagcatct gaagtagagg gagtagaggc gggcgttgaa gatgtcgatg
    10381 aaatccctgc tgtcgtgcgt aagcaact
//
LOCUS       DAOTMX010000016        10001 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_6276_length_10001_cov_3.719686, whole genome shotgun sequence.
ACCESSION   DAOTMX010000016 DAOTMX010000000
VERSION     DAOTMX010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 10001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10001
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6276_length_10001_cov_3.719686"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1021)
                     /locus_tag="P5573_00935"
     CDS             complement(<1..1021)
                     /locus_tag="P5573_00935"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRT26664.1"
                     /translation="MNLGKMLDETCRLFPERTALIYDDKTLTYKELKAAVDSLTRRLH
                     DLGIRKDDKVALMLPNVPEFVISYFAALKLGAVVVTLSTQSTSHELDYLLKNSDSRIL
                     ITTGQLSTRVDPIRKDLPLLADVLITEGMGESSPFMDAVEAGPSEIDRPEVHGDDPAV
                     MIYTAGLTGKPLGAVLTHNALLTQGDLLRHVCDGDENDRALAVIPLFHAFGASANMLN
                     ALKTGAGLVLMDVFNIDSLFKTVMDLQVTYIAAVPRIFLGMILHPTADKYNLKSLRFC
                     ITGGSAMPPEFIPLFEQKFGVPLREGYGLTEAAPICTLSRVDSEGRPGSIGTVIPGVE
                     ARIVDE"
     gene            1182..2249
                     /locus_tag="P5573_00940"
     CDS             1182..2249
                     /locus_tag="P5573_00940"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HRT26665.1"
                     /translation="MVNLLENVGNILIIQLGDIGDVVWATPTLRAVKRAHPGARLSVL
                     VRDGIGCLLAADPSLHRVYEVKRRKGNLFKRILLETDFIRQLRRQRFDIAVDLRLDER
                     GAYMAFASGAPVRVRLYNRDVPYLQNRLFTHLLTPPPRSAKARGAAEQSLALVREMGV
                     DTEDIVPRLWVADGSRRRAREILRDAGVVGGLRWVSINPFSRWTYKEWGNDKWARIID
                     WLGDEYRMAVVLVGSGEERERAADLVGRTKGKVFNLAGRTTLDELAALLELSCLNIGV
                     DSAAPHIAAAVGTPTITLYGPSDWEEWAPVGDGHHVVVPDLDCVPCYKKGCDGKGVSL
                     CLEDLTVERVEEGIRKALAGL"
     assembly_gap    2251..2350
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2423..3283
                     /gene="folD"
                     /locus_tag="P5573_00945"
     CDS             2423..3283
                     /gene="folD"
                     /locus_tag="P5573_00945"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041584935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HRT26666.1"
                     /translation="MAIIIDGNKTAADIRREIKEETAKLKRERGILPGLAVILVGEDP
                     ASAIYVRRKKNACEEVGFLSREYRLAADTEEEEVVRLIRSLNRDPAIHGILVQFPVPR
                     QIRAVAVIEAVDPAKDVDGLHPLNTGRLVAGFPSLVPCTPSGIIELLDRSGVEVEGKE
                     AVVVGRSNLVGKPLSLLLLSRNATVTVCHTRTRDLGEVARRADILVAAAGKAEMVRGD
                     MVKEGAVVVDVGVNRLADGRLVGDVAFIEAAEKASYITPVPGGVGPMTIAMLLRNTLS
                     AMRRLSDEAD"
     gene            complement(3266..4432)
                     /locus_tag="P5573_00950"
     CDS             complement(3266..4432)
                     /locus_tag="P5573_00950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRT26667.1"
                     /translation="MVERFESPLKRYRRRLRAWGNHRKLEREREYYNELFLSRGLVLP
                     DEEAVRAALKKKYPRLTSRRKGSLHILALYHHYNWEDYSLMPALERFGSVRHYDWCEE
                     FDHQGKDWPVSGKKAMNAALVERAQKWVREDRIDVIFSYLSGEQVFPETVARISSLGV
                     PTVNLSLNDKEHFVGRIRRGLAMGMRDICRHFDLCWTSTADALVKYRVEGALPVYLPE
                     GANPEIHRPYDVEKTIDVSFVGQCYGNRPEVVERLRRRGVAVEAFGYGWPSGPLTTEE
                     MVRMYSRSRINLGFGGVAGHRETFCLKGRDFEIPMSGGLYLTEHHDELECVYRIGSEI
                     VTYENFADLAEKIGYLLSHPEEAEAIRQRGFRRARAEHTWEMRFEKVFGLIGLI"
     gene            complement(4426..5154)
                     /locus_tag="P5573_00955"
     CDS             complement(4426..5154)
                     /locus_tag="P5573_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT26668.1"
                     /translation="MMKQDFRADFFEILRMIVESDSLALSRFADGEANVLKNNTIGNK
                     DGWLYKKDKNLVFRRDLRHSLLCTDPNFLYGISCTCCDKENHRYLLENIKTGIENLTF
                     SNIWVNANYPLFQEKFFDTLVAAKKDIASCTNKKAAVMNLERTLPIKDFIPIPGNCVT
                     YWEKNRDYIRALLDLKGSTHRNRIYLFAAGPLSEILIHELWRVNPNNILLDIGSTLDP
                     LLFRRKSRDYHRGNSLFSNRTCTW"
     gene            complement(5159..6205)
                     /gene="wecB"
                     /locus_tag="P5573_00960"
     CDS             complement(5159..6205)
                     /gene="wecB"
                     /locus_tag="P5573_00960"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HRT26669.1"
                     /translation="MILIAYGTRPEIIKLFPVVDALKSEGIPFKTLFTGQQTDLYEDV
                     KDLVPAADFSFADAFSGEGKHNTLGMSFIKICEASERLLSDRSFDLVLVQGDTTTAWA
                     LSQMAFYNGIKVAHVEAGLRTYDLENPYPEEANRILIDHVAFLNFAPTDRACRNLRKS
                     GAKGVYLVGNTVVDAVNHFKDSLRLVNETSNVVLVTLHRRENHDIMAYLFDELNEVAA
                     HNPELDFVLPIHPNPNVNRHRTRLTATNIRVVRPVSYPDMLRLMSRASFLISDSGGIQ
                     EEATCFNKKVIILRKVTERPEVVDMGLGVLAGRDIGKHIEWAKVPPEPAVKSPYGKGD
                     AAKKIARILREKMG"
     gene            complement(6202..6945)
                     /locus_tag="P5573_00965"
     CDS             complement(6202..6945)
                     /locus_tag="P5573_00965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRT26670.1"
                     /translation="MKPKISVILTAWARPQYIEEQVERVLEQTVPPHEIVLWYNNPPP
                     KKGFLEMKQRVNFKNEKHVKKILCDHNFGIIPRFAIAPAMEGEYVCIFDDDTMPGERW
                     LENCLNYVDKEKVLCGTIGLRYISRTELKTEKPRMGWEAMNRTLEFVDLVGHSWFMRR
                     EWARYFWDEEPLLRHFGEDIHLCAMLQRHGIRTACPPHPPDDRSLWGSVKPELGIDKV
                     AISISSDKSKDFWNVVKYELEKGYKPILL"
     gene            7021..7533
                     /locus_tag="P5573_00970"
     CDS             7021..7533
                     /locus_tag="P5573_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT26671.1"
                     /translation="MIDFVASPSVGEVPIRFRITVKSTPILAFLKEKPFFLKTYHEIK
                     RQARESREDFPFGEFYPCLEDRYKESGEASGHYFHQDLLVASMIFNNKPQRHVDVGSS
                     MNFVAHVASFREVGVFDIRGQRSLFENIRFRRLDLMEEGFDLVDYCDSVSCLHVLEHL
                     GLGRYGDRVD"
     gene            7675..7821
                     /locus_tag="P5573_00975"
     CDS             7675..7821
                     /locus_tag="P5573_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT26672.1"
                     /translation="MEERYRVDSFSYVDDGGDLVRDADLSADAVGKNFNCHYGCGIFE
                     LTKA"
     gene            complement(7785..8606)
                     /locus_tag="P5573_00980"
     CDS             complement(7785..8606)
                     /locus_tag="P5573_00980"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HRT26673.1"
                     /translation="MKNFLLKVLPWNRHLYSFCKSYVERHDGDCNGDMMTNGEFRTIE
                     HFLPGCTVVFDVGAHTGEWTNLALTINPNLTVHCFEPCRESFEKLTANISSDRVILNN
                     FGLSSAQGEKPLFIYPDAPGLNSLYQRRGLEEGWGLGSSGKTETIELDTLEHYCSERC
                     IPQVDFLKVDVEGHEMEVFKGGASLFRENRIQVVQFEYGGCNIDSRVLLKDIFEFFDG
                     TAYRFYKIMPDTLRPVERYDQRLENFKYQNWLIVQMKSAFWNPLQAFVSSKIPQP"
     gene            complement(8651..9541)
                     /locus_tag="P5573_00985"
     CDS             complement(8651..9541)
                     /locus_tag="P5573_00985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRT26674.1"
                     /translation="MENRVEISVIIVSHNACGVVGTCIDSVIGIDDAKKKVFVVDNAS
                     TDGTAGFIRENYPSVNLIANEKNTGFAAAVNLALKSCKGRYIFLLHPDTEFLPSSFRP
                     LISFMDGRPHVGLAGLKIFNPDGTVQESFSAEYPGQKYAKGELLGLNGSLAWALSAGM
                     VVRADVMRQVDGFDEDFFIYGGDWDLCLRVRKAGYGIDLIEAASLVHLGGQGERQALT
                     ADSLKRRLIAEYTFYEKHYRPETVEKITKSYLMEAGRQILKLRLLAPFYRDRKDFAER
                     YARCQAIRHVLKSDTLKKDT"
     gene            complement(9554..>10001)
                     /locus_tag="P5573_00990"
     CDS             complement(9554..>10001)
                     /locus_tag="P5573_00990"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRT26675.1"
                     /translation="LVAGKGNVKRYRSLARKLGIEENVVFAGVADRETLDKMYLAADM
                     FSILSQFDTFSMTTLEAMAASLPVMISGNVGAKDLVKEGVNGFIVEDVNNAEQIADKI
                     EKMSDDSVRVRMAEEAFKTARAHTWEDAAGKIEEMYEEILRDKRRF"
BASE COUNT         1952 a         2690 c         2960 g         2299 t
ORIGIN      
        1 gctcgtccac aatccttgcc tccacgccgg ggataacggt ccctatcgac cccggccgtc
       61 cttccgaatc gacccggctc aaggtgcaga tcggcgccgc ctcggtcagt ccgtatccct
      121 ccctgagggg tacgccgaat ttctgctcaa acaggggaat gaattccggc ggcatcgccg
      181 acccgcccgt gatgcagaac cgaagggatt tcagattgta cttgtccgcc gtcggatgga
      241 gtatcatgcc gaggaatatc cgcggcacgg cggcgatgta agttacttgc aggtccatga
      301 ccgtcttgaa taaggaatcg atgttgaaaa catccatgag aacgaggccg gcccccgttt
      361 tcagcgcatt cagcatgttc gccgaggcac cgaaggcatg gaaaagcggt ataaccgcca
      421 acgccctatc attttcgtcg ccgtcgcaga catgcctcaa gaggtccccc tgagtcagca
      481 gggcgttatg tgtcagcacg gcgccgaggg gctttcccgt gagtccggcg gtatagatca
      541 tgactgcggg gtcgtcgccg tggacctcgg gcctgtcgat ttcggaagga cccgcttcga
      601 cggcatccat gaaaggagag gactcgccca tcccctctgt gatgagcaca tctgcgagga
      661 gcggcaggtc tttcctgatc gggtctactc ttgtcgataa ttggcctgtt gttatcagta
      721 tccgggaatc gctgttcttg aggaggtagt cgagttcgtg agaagtggac tgggtgctca
      781 gcgtcacgac gacggcgccg agcttcagtg cggcgaagta ggagatgacg aactcgggga
      841 cattgggcag catcagggcc accttgtcgt ctttccgtat gccgaggtcg tggagcctgc
      901 gggtaagaga atcgacggca gctttgagtt ctttatatgt cagtgtctta tcatcgtaga
      961 tgagtgctgt tctttctgga aacaaccggc aggtttcgtc cagcattttt cccaggttca
     1021 ttaccctgtc tccctcactt ttaaccgctt tctcatagca taggaaagac gaacctttca
     1081 actgaattat cacttgattc tgaagaatta tgttgttgac agcggggaaa ccgggtaata
     1141 accttgcgcc ctagcgagca ggcgtttcga cagggtagac catggtgaat ttgctggaga
     1201 atgtcgggaa tattctcatc atccagctcg gcgacatcgg ggatgtggtg tgggcgacgc
     1261 cgaccctgag ggccgtaaaa cgggcccacc ccggggcgag gctgtcggtg ctggtgaggg
     1321 acggcatcgg gtgtcttcta gcggccgacc cgtcgttgca tcgggtgtat gaagtcaaac
     1381 ggcgcaaggg aaacctgttc aagcgcattc tcctggagac ggatttcatc aggcaactgc
     1441 ggcgccaacg cttcgacatc gccgtcgacc ttcggctgga cgagaggggg gcctacatgg
     1501 cctttgcctc gggcgcccct gtgagggtaa ggctgtacaa ccgggatgtt ccttacctgc
     1561 agaaccggct tttcacccat ctcctcactc cacctccgcg ctcggccaag gccaggggag
     1621 ccgccgagca atcgctcgcc ttggtgagag agatgggagt cgatacggag gacatcgtgc
     1681 cgaggctctg ggttgccgac ggaagcaggc gccgggcgcg cgaaatcctc cgtgatgcgg
     1741 gcgtcgtcgg cgggcttcgg tgggtcagta tcaacccctt ttctcgctgg acatacaagg
     1801 agtggggcaa cgacaaatgg gcccggatca tcgactggct cggggacgag taccggatgg
     1861 cggtcgtttt ggtcggttcc ggcgaggaga gggaaagggc ggcggacctc gtcgggagga
     1921 cgaaaggcaa ggtgttcaac ctggcgggta gaaccacgct cgacgaactt gctgccctgc
     1981 ttgagctgag ctgtctgaac atcggcgtcg acagcgcggc cccccacatc gcggcggccg
     2041 tagggacgcc gacgatcacg ctgtacggac cgtcggattg ggaagaatgg gcgccggtcg
     2101 gagatgggca ccatgtcgtc gtgcctgacc ttgactgcgt tccctgttat aagaaagggt
     2161 gcgacggcaa gggcgtgagc ctctgcctgg aggatttgac ggtggaaagg gtcgaggagg
     2221 gaatccgaaa ggcgttggcg ggactgtgac nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn ttgacggtgg aaagggtcga ggagggaatc cgaaaggcgt tggcgggact
     2401 gtgactgacg ggagggagag gaatggccat catcatcgac gggaataaga cagccgccga
     2461 catcaggcgg gaaataaaag aagaaacggc aaaacttaaa cgagaaaggg gcatccttcc
     2521 cggtctcgcc gtcatcctcg tcggtgaaga cccggcctcg gccatatacg tgaggagaaa
     2581 gaagaacgcc tgcgaagagg ttgggtttct gtcccgcgag tacagattgg cggctgacac
     2641 ggaggaagag gaggttgtcc gtttgatacg gtcgttgaac cgggaccccg cgattcacgg
     2701 catcctggtt cagttccccg tgccgcgaca gataagggcc gtcgccgtga tcgaagccgt
     2761 ggacccggcc aaggacgtgg acgggctgca ccccctgaat acgggacgtc tcgtcgccgg
     2821 tttcccgtcc ctcgttccct gcacgccctc cggaataatc gaactcctcg accggagcgg
     2881 cgtcgaggtc gaagggaagg aggccgtagt ggtcgggagg agcaatctcg tcggaaaacc
     2941 cctctccctc ttgctccttt cgcgtaacgc aacggtaacc gtctgccaca caaggacccg
     3001 ggacctcggc gaagttgcgc ggagggccga catcctcgta gcggcagcgg ggaaggcgga
     3061 gatggtgaga ggagacatgg taaaagaagg cgccgtcgtc gtcgacgtgg gcgtcaaccg
     3121 gcttgcggac ggccgtcttg tcggggacgt ggccttcatc gaagcggcgg aaaaggcgtc
     3181 gtacataaca cccgttcccg gaggcgtcgg gccgatgacc atcgccatgc tcctcaggaa
     3241 cacgctgagc gcgatgcgcc gtctttcaga tgaggccgat taggccgaag accttctcga
     3301 aacgcatctc ccaggtgtgc tcggcacggg cccgccggaa ccccctctgc cgtatggcct
     3361 ccgcctcctc ggggtgcgaa aggaggtagc cgattttttc tgccaggtcg gcgaaatttt
     3421 cgtacgtcac gatttcgctc ccgattcggt aaacacactc cagttcgtcg tggtgctccg
     3481 tgagataaag gccgccgctc atcggtatct cgaagtccct ccctttgagg cagaaggtct
     3541 cccggtgacc cgccacgccg ccgaagccga ggttgatgcg gctccgggag tacatcctca
     3601 ccatttcttc cgtggtcagt ggcccgctcg gccagccgta gccgaaggct tccacggcga
     3661 cccctcttct tctcagtctt tcgacgacct cggggcgatt tccgtaacat tgacccacga
     3721 aggatacgtc gatggtcttc tcgacatcat aaggcctgtg tatctccggg ttggcaccct
     3781 cggggaggta gacggggagc gccccttcga cgcggtactt cacgagggca tccgcagtgc
     3841 tcgtccagca gaggtcgaag tggcggcaga tgtcgcgcat ccccatcgcc agaccccggc
     3901 ggatacgccc gacgaagtgt tccttgtcgt tgagggagag gtttaccgtc ggcacgccga
     3961 gggaacttat tctcgcgacg gtttccggga agacctgttc cccggagaga taggagaata
     4021 tgacgtcgat ccggtcttcc ctgacccact tttgcgcccg ttcgacgagt gcggcgttca
     4081 tcgctttttt cccggagacg ggccagtcct tcccctggtg gtcgaattcc tcgcaccagt
     4141 cgtagtgacg cacgctgccg aatctttcca gggccggcat caaggaatag tcttcccagt
     4201 tgtagtggtg gtagagggcg aggatgtgca atgaaccttt gcgccttgag gtcaggcggg
     4261 gatatttttt cttcaatgcc gcccggacgg cttcttcatc tggcagcaca aggccccggg
     4321 aaaggaagag ttcgttgtag tattcccgct ctcgttccag tttccggtgg ttcccccacg
     4381 cccggagccg tcgccggtac cttttcaggg ggctttcgaa tctctctacc atgtgcacgt
     4441 gcggttggag aagaggctgt tcccacgatg gtagtctctc gatttccttc ggaacagcag
     4501 cggatcgagc gtagagccga tgtcgaggag gatgttgttg ggattgacac gccagagttc
     4561 gtgaatcagt atttccgaaa gcggccccgc cgcaaagagg tagatcctgt ttctgtgcgt
     4621 gctccctttc aggtccagta gggcccggat atagtcccgg ttcttttccc agtaagtgac
     4681 gcagttgccg ggtatcggga tgaagtcttt gatcggcagc gttcgttcga ggttcattac
     4741 cgcggccttc ttgtttgtgc agctagcgat gtcctttttt gccgcgacga gggtgtcgaa
     4801 aaatttttcc tggaaaaggg ggtagtttgc gttcacccag atgttggaga acgtgaggtt
     4861 ctctatgccc gtttttatgt tttccaggag gtatctatga ttctctttgt cgcagcaggt
     4921 gcaggagatt ccgtagagga aattcgggtc cgtgcagagg agggaatgcc tgaggtctct
     4981 ccggaagacg aggtttttgt ccttcttgta caaccatccg tccttgttgc cgatggtgtt
     5041 gtttttcagg acgtttgctt ccccgtcggc aaaccgggag agggcgaggg agtcgctctc
     5101 gacgatcatc cggaggatct cgaaaaaatc ggctcggaag tcctgtttca tcatcttgtc
     5161 atcccatttt ctctcttagg atacgggcaa tcttctttgc cgcgtcccct tttccgtacg
     5221 gcgatttcac cgccggttcc ggcggaacct tcgcccattc gatgtgtttt ccgatatccc
     5281 gaccggcaag taccccaagc cccatgtcca cgacctcggg gcgttctgtc acctttcgca
     5341 ggattatcac ctttttgttg aagcaggtcg cctcttcctg gatacctccg ctgtcggaga
     5401 tgaggaacga ggcgcggctc atgagcctca gcatatcggg gtaacttacc ggccggacaa
     5461 ctcttatgtt ggttgccgtg agccgtgtgc ggtgcctgtt gacgttgggg ttcgggtgga
     5521 tcgggaggac gaaatcgagc tctgggttgt gggccgccac ctcgttcaac tcatcgaaaa
     5581 ggtacgccat tatgtcgtga ttttcccttc ggtggagggt gaccaggacg acgttcgatg
     5641 tctcattcac cagccggagg ctgtccttga agtgatttac ggcgtcgacg accgtatttc
     5701 ccacgaggta gacgcccttc gcgcccgatt tccggaggtt tcggcaggcg cggtccgtgg
     5761 gcgcgaagtt gaggaaggcg acgtggtcga tcaaaatccg gttcgcttct tccgggtagg
     5821 ggttctctaa gtcgtaggtc cgcaagcccg cttccacgtg ggcgaccttg atgccgttgt
     5881 agaaggccat ctgggatagt gcccaggccg tggtcgtatc accctggacg aggaccaggt
     5941 cgaaagaccg gtcggagagg agcctttccg atgcctcgca tatcttgatg aagctcatcc
     6001 ccagggtgtt atgtttgccc tctcccgaaa aagcatcggc aaagctgaag tcggccgccg
     6061 ggacgaggtc cttgacgtct tcgtagaggt cggtctgttg ccccgtaaac aacgtcttga
     6121 aggggatgcc ctcggacttc agggcgtcga cgacgggaaa gagcttgatg atttcggggc
     6181 gtgtgccgta ggcgatcagg atcatagcag tataggcttg taacccttct ccagttcgta
     6241 tttcacgacg ttccagaagt ctttcgactt atcggagctg atgctgatcg cgaccttgtc
     6301 gatgccgagt tcgggcttca cggagcccca gagggacctg tcgtcgggcg gatggggagg
     6361 acaggctgtg cggatgccgt gtctttgcag catggcgcag agatggatgt cttcgccgaa
     6421 atggcggagc agcggttctt cgtcccagaa ataccttgcc cactcgcgtc tcatgaacca
     6481 actgtgtccc acgaggtcga cgaactcgag ggtcctgttc atcgcctccc atcccatccg
     6541 cggcttttcg gtcttaagct cggtcctgga tatataacgc aggccgatcg tgccgcaaag
     6601 cactttttct ttatccacgt agttgaggca attttccaac cagcgctcgc ccggcatggt
     6661 gtcgtcatca aagatgcaga cgtattctcc ctccatggcc ggagctatgg cgaaacgggg
     6721 gatgatcccg aaattgtggt cgcagaggat cttcttcacg tgtttctcgt tcttgaaatt
     6781 cacccgctgc ttcatctcca ggaacccttt tttgggggga gggttgttgt accagaggac
     6841 gatttcatgg gggggaaccg tctgttccag gacccgttcc acctgttcct cgatgtactg
     6901 gggcctcgcc caggcggtca atatcaccga tattttaggc ttcatagata cacatacaac
     6961 ttgcggagtg gaccccggtc acggcgcggg ttgaatccat tagaccgctt gcgctcaaat
     7021 atgatagatt tcgtcgcgag tccttcagtc ggagaagtac ccatacggtt caggatcact
     7081 gtcaaaagca cgcctatttt agcttttctg aaagaaaagc ctttttttct gaaaacctac
     7141 catgaaatca agaggcaggc aagggaatcg agggaggatt ttcccttcgg tgaattttat
     7201 ccctgcctgg aagataggta taaagaaagc ggcgaggcgt cgggccatta ttttcatcag
     7261 gatttgttgg tcgcttcgat gatcttcaac aacaagccgc aaaggcatgt ggatgtcggg
     7321 tcgagcatga actttgtcgc ccatgtggcg tcgttcagag aggtcggggt cttcgatatc
     7381 cgcggtcaaa ggtctctttt tgagaatatc cggttcaggc gcctcgacct gatggaggaa
     7441 ggtttcgatt tagtcgatta ttgcgattcg gtctcttgtc tgcacgtatt ggagcatctc
     7501 ggcctgggca ggtacggcga cagggtggat taggaagggc atctgaaggg gtggaaaaac
     7561 atccacacga tgctccggaa aggtgggaag ttctattttt tcggcaccca tagggacgaa
     7621 gaggatcgaa ttcaacgccc acagggtctt ttccttcagg tatcttctat gtctatggag
     7681 gaaagataca gggtcgattc gttctcgtac gtcgatgacg ggggagacct tgtccgggac
     7741 gcggacctgt cggcagatgc cgtggggaaa aatttcaact gccactacgg ttgcgggatc
     7801 ttcgagctga cgaaggcctg aagagggttc caaaaagcgg atttcatctg gacgatcagc
     7861 cagttttggt atttgaagtt ttccagccgc tggtcgtacc gctctaccgg cctcagggtg
     7921 tcgggcatga tcttgtagaa gcggtaagcc gttccgtcga agaactcgaa gatatccttc
     7981 agcaggactc tggaatcgat gttgcagcct ccgtactcga attggacgac ctgtatacgg
     8041 ttttcccgga acagggaggc gccccctttg aagacttcca tctcgtgtcc ctctacgtct
     8101 actttcagga agtcgacctg cggtatgcac cgctcagagc agtaatgctc aagggtgtcc
     8161 agctcgatcg tttcggtctt tcctgacgac cccagtcccc acccttcttc cagtccccgt
     8221 ctttggtaga gggaattcaa ccccggcgcg tcggggtaga tgaagagggg cttttcgccc
     8281 tgggcggagc tcaagccgaa attattcagg atgacgcggt ccgatgagat gttcgcggtg
     8341 agcttctcga aactttctct acacggttcg aaacagtgga cagtcaggtt cggattgatc
     8401 gtcagggcga gatttgtcca ctctcccgtg tgtgcgccta cgtcgaacac cacggtacac
     8461 cccggcagga aatgctcgat ggtgcggaat tcgccgttgg tcatcatgtc gccgttgcag
     8521 tctccgtcgt gcctttcgac gtaactctta caaaagctgt acaagtgccg gttccacggc
     8581 agtaccttga ggaggaagtt tttcattgcc tatcaccgtt caaaaagtgg gttcggtgaa
     8641 attactctaa tcaggtgtcc tttttcaagg tgtcgctttt gagcacgtgg cggatggcct
     8701 gacagcgcgc gtatctttcc gcgaaatctt ttctgtcgcg atagaacggg gcaagcaacc
     8761 tcaacttcaa aatctgccgt cccgcctcca tcaggtagct cttggttatc ttctcgaccg
     8821 tctccggccg gtagtgtttt tcgtagaagg tgtactcggc gatgagcctt cttttcaatg
     8881 aatcggcggt tagggcttgc cgttcgccct gtccgccgag atggacaagg gatgccgcct
     8941 cgatgaggtc gataccgtac ccggccttac gaaccctgag gcagagatcc cagtctccac
     9001 cgtagataaa aaagtcctcg tcgaagccgt cgacctgcct catgacatcg gcgcggacga
     9061 ccatcccggc gctgagggcc caggcgaggg aaccgttcaa tcccaaaagc tcgcccttcg
     9121 cgtacttttg accggggtat tccgcagaaa aggactcctg gaccgtgccg tcggggttga
     9181 atatcttgag ccctgcgagc ccgacgtgcg gccggccgtc catgaaagaa atcaacggcc
     9241 gaaaggacga cggaaggaat tctgtgtccg gatggagtag gaagatgtac ctgcctttac
     9301 aggatttcag ggcaaggttg acggccgccg caaaacccgt gttcttctca ttcgcgataa
     9361 gattgacgga agggtaattt tccctgatga agccggcggt tccgtccgtg gaagcattgt
     9421 cgaccacgaa aacttttttt ttggcgtcgt cgatgccgat gacggaatcg atacaggtgc
     9481 cgaccacgcc gcacgcgttg tgcgacacta tgatgacgga tatttcaacc cgattctcca
     9541 tcccgcggtc ctctcagaaa cgccgcttgt ccctcaatat ctcttcgtac atctcctcga
     9601 tttttccggc cgcatcttcc catgtgtgtg cgcgcgcggt cttgaatgcc tcttccgcca
     9661 tacgcacacg gacggaatcg tctgacatct tttcgatctt atcagcgatt tgttccgcgt
     9721 tgtttacgtc ttcgacgata aaaccgttga cgccttcttt tacgaggtct tttgcgccca
     9781 cgttgccgct tatcatcacc gggagcgatg ccgccatggc ctcgagggtt gtcatgctga
     9841 aggtgtcgaa ctgagatagg atagagaaca tatcggccgc aaggtacatc ttgtcgaggg
     9901 tttccctgtc ggcgaccccg gcgaagacga cgttttcctc gattccgagc ttccgggcca
     9961 gggagcggta cctcttcacg ttccctttcc cggcgacgag g
//
LOCUS       DAOTMX010000017         9953 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_6303_length_9953_cov_4.910285, whole genome shotgun sequence.
ACCESSION   DAOTMX010000017 DAOTMX010000000
VERSION     DAOTMX010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 9953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9953
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6303_length_9953_cov_4.910285"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /gene="purB"
                     /locus_tag="P5573_00995"
     CDS             complement(<1..889)
                     /gene="purB"
                     /locus_tag="P5573_00995"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HRT26676.1"
                     /translation="MIPRYSRPKMASLWSAEERYRRWLDIEILACEALAAKGVIPEKA
                     LKNIKEKARFDVARVEEVEKTVKHDVIAFLTSVGEYVGEDSRYIHMGLTSSDILDTSL
                     ALLLRDAAALLVEDIDRLLSVLKRRALEHKKTVMIGRSHGVHAEPITFGLKMALWYQE
                     MKRNKGRVLRAGEVIGYGKLSGAVGTYAFIDPSVESYVCGKLGLKPAPVSSQIVQRDR
                     HAEYFAQLAVVAASIEKFATEIRHLQRTEVREAEEYFSPGQKGSSAMPHKRNPVLAEN
                     LSGLARLMRSYAVAAMEDVA"
     gene            1050..1493
                     /locus_tag="P5573_01000"
     CDS             1050..1493
                     /locus_tag="P5573_01000"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRT26677.1"
                     /translation="MQTDDRLIYLLFTAGQKLRNYLNSALSAEGAKVTVAQSGILFLL
                     KQQNGRSMTELSQLMELDNSSITGLIDRLERAGFVSRNSDPADRRLSNVYITPRGLEE
                     VNKAKVVIRKVNEEIKEGFTAEQVDTFKSVLKSFSPKFNSKLPSR"
     gene            1542..2681
                     /locus_tag="P5573_01005"
     CDS             1542..2681
                     /locus_tag="P5573_01005"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRT26678.1"
                     /translation="MAMTGHTRDYQKEFSRFAEKEIASRRDLHTEDDFPLGIWRKMAS
                     AGMLGVSIPRAYGGLGGGYAAVVAAGEGFVESGHNMGLALSWVIHQVASRFFIHGFGT
                     ESQRERYLPALAEGTATASIAISEPGVGANPKNLKTSAVPEGGGYLVQGEKSYLTNGP
                     IADLFIVFAVTSESDGRKEYTAFLVPKDTPGLSLTEPLKLDYLRPSPHCGITVDSCRV
                     RATDVLGEVGSAYRKMVIPFRDLEDALLMGPVIGGMKAQIDFLVTAYMEATVPATDGI
                     KTELGALDFLARTMRVCSYQVARMIDSGMDTEAYQPLLLSLRYLAKDFQSRVERLAGV
                     TGLSNQGEFKALTTDIGKIINIAGNVALIKQKKLGERLLSQKGEA"
     gene            2678..3622
                     /locus_tag="P5573_01010"
     CDS             2678..3622
                     /locus_tag="P5573_01010"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRT26679.1"
                     /translation="MKQKKDEKFTSIRHIQEGFKKVGYITSRQTATTVYLAHHLEKPI
                     LVEGPAGVGKTELAKTIAAYLDLPMIRLQCYEGLDESKALYEWKYGKQLLYTQLLRDK
                     LDSLVDREDSFSQSINKLHAYDDIFFSWHFLEPRPLLKALQEKDGTVLLIDEVDKADD
                     EFEAFLLEVLSDFQVSIPEIGTVHAVTKPLVVLTSNNTRELSEALKRRCLHLFIPFPE
                     PALEREIIRVRVPEIPSNLAAQLVSFIQAVRKMDIKKVPSISETIDWARVLLILHADS
                     LSPDLVRDTLHIFIKYEEDIRVVEDQLHQLTANAARAF"
     gene            3641..4975
                     /locus_tag="P5573_01015"
     CDS             3641..4975
                     /locus_tag="P5573_01015"
                     /inference="COORDINATES: protein motif:HMM:NF017569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRT26680.1"
                     /translation="MDRVLEDFITTLRRSGVRISVSESLDAARALQLVSYDDRSLLKD
                     SLYVALAKSVQEKEIFSDCFDRFFSFDFFSEPPPNEGSHKGSTEGLSDLSRMLMTGDQ
                     PGLVTYMKEAARAENLSDIRYQTQKSLYAMRILERMGLSGLDADIRDLYQAGAPSSAA
                     TAARLEEARSYLVENVRSYVEEQLLLFSGYEYDKMIEDYLRNIKLSNLEQRYYHQMQG
                     IIQRMVKRLNDLYSRKRKPARRGHLDFKQTIRDNVAYQGFLFNPRWKKVKIEKPNVVA
                     ICDISRSVSRLVRFFLLFLYGLNKEIIQVRSFIFCSNLAEVTGIFEQYPIEEAVARIQ
                     AGTDIPVLLARTDYDRTLRDFKERYLDAVTHKTTVLFLGDARNNYNNPQTQILKLLSE
                     RCKKLIWLNPETKPFWGTGDSEMLRYAPYCHVLKECNTLNHLERVVQSLLKR"
     gene            5108..5869
                     /locus_tag="P5573_01020"
     CDS             5108..5869
                     /locus_tag="P5573_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT26681.1"
                     /translation="MDTDKEFFDPRYREFKELTDRIAAEVGLPLFYSEEKDAVERSRR
                     IFSSHPAAEELLAYLGNNEEYPGHGLYHVKKVALDAGALILIDGADAVGVDRVDRLVF
                     LSHLAGLLHDIRRSEPNHARRSAEEAGRILKTFDIETLERDFIIQAIANHEAFQPVQP
                     LDDPSAQMLSNALYDADKFRWGPDNFTETLWAIIMSRRLKLEDLAENFPGGLAATERI
                     RGTFRTRTGKKYGPDFIDRALEIGNRLFAFLSSHA"
     gene            complement(5947..6023)
                     /locus_tag="P5573_01025"
     tRNA            complement(5947..6023)
                     /locus_tag="P5573_01025"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5987..5989),aa:Met,seq:cat)
     gene            complement(6093..7832)
                     /locus_tag="P5573_01030"
     CDS             complement(6093..7832)
                     /locus_tag="P5573_01030"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HRT26682.1"
                     /translation="MRRILISLVCLTLMVGVFLVARECIVSAADDTKVKRVAVLPFDV
                     HSSEDIDYIRNGVWDMLISRISLAGKIEVVSKNDVQNVLAKRKGKGNLTVADVYELGK
                     KMGADYVVHGSITKIGNSVSLDGKLLDVAANRSAVDVFAQSQGMDDVIPKIGDFAKRI
                     DYHILGQVPATFGAPPPSPVDSLAVASPQPPPAGQAPTDEAIGAVRTPQGTFTAVINP
                     EFINAPQTAIDKKGFWMSQRFETEFKGMDVGDVDGDGKNEVVTVDAYNVYVYRKSGKT
                     LTLLQKIAGKSYDQYLSVDVADVTGSGKKEIIVTSVNRGILDSFVLQYKNGKYTTIAK
                     DLRWFMRVINISDTPTLLGQRMGSEKPFENPIYEIVWSKGRLKEGKRMEIPQGLSVYG
                     LAIEPLEKDGPNKIIALDNLDYLRVFEPTVKSIDKILTFGGSKEMIWKSDEAFGGSNN
                     FFDLPSDAQISKNIAATEEKARPYVNIRLLAYDINKNGKKDLLVVKNISTVGRLFQNM
                     KIFSSSEIYDLEWNGLGFAENWRTKKIQGYVADYQIKDIDNDGKLDVVLSLVLSYDMG
                     ITRRSVVVAYALS"
     gene            8088..>9953
                     /locus_tag="P5573_01035"
     CDS             8088..>9953
                     /locus_tag="P5573_01035"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRT26683.1"
                     /translation="MPLDHWRSVSPEKFASEDVIFRHIHRGDTIFIGSACAEPQYLVQ
                     GLIRYVKAHPKAFFDAEVLHIRTLGVAPYATEKFRDNFRHNSFFIGDSTREAVNKGLA
                     DYTPVFLSQVPDLFRRGLARVDVALIQTSSVDIHGYMSLGVSVDIVKAAVETASVVIA
                     QVNSRMPRVHGDAFIHIDDIDFVVPRDEEILEYSPEADTEAAQRIGKYVARLIEDGDT
                     IQVGYGSIPNAILANLSEKKHLGVHTELISDGVADLMKKGVIDNTRKNINRGKTVATF
                     CMGRRATYDYIHDNPAVEFRTIDYTNDPLVIAQHDGMVAINSALEIDLTGQATAESIG
                     RTFHSGVGGQADFMRGAVLSRGGKTILALKSTAASDVVSRIVPALREGAGTTLIRGDI
                     HYVVTEYGIAYLHGKNIRERAMELIAIAHPKFRPGLISEAKKAGLIYQDQAFIPGERG
                     IYPEELETWRTTKTGLNIFLRPIKISDEPLLKDFLYSLSDKSLYRRFMSQRFHMPHEF
                     LQELVVIDYTREMAILAVVGETPNEKVVGVGRYFIDPETHTAEVAFAVRDDFHNQGIG
                     SELLSYLTYLAKRQGLLGFTAEVFADNAPMLHVFEKGGFDIQKRTIAGLCELKMTF"
BASE COUNT         2229 a         3040 c         2592 g         2092 t
ORIGIN      
        1 gcgcgacatc ttccatggcc gcgacggcgt acgacctcat caggcgggcg agcccggaga
       61 ggttttccgc gaggaccgga ttcctcttat ggggcatggc cgaggaaccc ttctggcccg
      121 gtgaaaagta ctcttccgct tcgcgcacct ccgtcctttg caggtgccgt atctccgtcg
      181 caaatttctc gatagaggcg gcgacgaccg ccagttgggc gaagtactcg gcgtgcctgt
      241 cccgttgaac gatctgggac gagaccgggg ccggcttcag gccgagcttc ccgcacacgt
      301 aagattcgac ggaggggtcg atgaaggcgt aggtcccgac cgccccggaa agcttgccgt
      361 aaccgatgac ctcgcctgcc cggaggacgc ggcccttgtt tcttttcatc tcctggtacc
      421 acagggccat cttaagcccg aaagtgatcg gctcggcgtg tacgccgtgc gacctcccga
      481 tcatcacggt tttcttgtgc tccagcgccc tcctcttcag gaccgagagg agccggtcga
      541 tgtcttcgac caagagggct gccgcgtctc tgagcaagag ggcaagcgac gtgtccagta
      601 tatccgatga ggtcaatccc atgtggatgt accgggaatc ctctcccacg tactcgccga
      661 ccgacgtaag gaaggcgata acgtcatgct tgacggtctt ttcgacctcc tccaccctcg
      721 caacgtcgaa acgggccttc tccttgatgt tcttcaaggc cttctccggg atgacacctt
      781 tggccgccag cgcctcacag gccagaatct ctatatcgag ccagcgcctg tacctttctt
      841 cagcgctcca caggctcgcc atcttcggcc tcgaataccg tgggatcatg ccgacctccc
      901 tttttaaaag ataaacctca tgaatgtccg tgccttgttt ttctattatg acggtgggtg
      961 gtagtcaagt ttttattcct gattttcgtt gacagcggat gtttgatatg ccaaatatgt
     1021 gtctaccaaa catcggggac ggtctttcca tgcagacaga cgacaggctc atctaccttc
     1081 ttttcacggc aggccagaaa ctgcggaatt acttgaacag cgctctgtcc gccgagggcg
     1141 cgaaggtgac cgtcgcccaa tcaggcatcc tcttcctgct caagcagcaa aacggccgct
     1201 cgatgacgga attgagccag ttgatggagc tggacaattc gtcgatcacc ggcctgatcg
     1261 accgcctcga aagggctggc ttcgtgtccc gcaattcaga ccccgccgac aggcgtctat
     1321 ccaacgtcta catcactccg agggggctcg aagaggtcaa caaggcgaag gtcgtcatcc
     1381 ggaaagtgaa cgaagagata aaagagggat tcacggcgga acaggtcgac acattcaaga
     1441 gcgtcttgaa gagcttttca ccgaagttca acagtaaatt gccatcacgt tagaccgacg
     1501 gccaaagaag gtttgctaag gcagcccgcc gggacatcac tatggccatg accggacata
     1561 ccagagacta tcagaaagag ttttcccggt ttgcggaaaa agagatcgcc tctcgccggg
     1621 acctccatac cgaagacgac tttcccttag gcatatggcg taagatggcg tccgcgggga
     1681 tgcttggggt atccatcccc cgtgcgtacg gcggtctggg cggtggttat gccgccgtcg
     1741 tcgcggccgg agagggcttc gtcgaaagcg ggcacaacat gggtctcgcc ctctcctggg
     1801 taatccacca ggttgcctcc cgtttcttca tccacggttt cggcacggaa agccagcgcg
     1861 aacgttacct cccggccctg gccgaaggca ctgcgacggc gagcatcgcc atatcggaac
     1921 cgggtgtggg cgctaatccc aagaatctga agacctccgc cgtacccgaa ggaggggggt
     1981 acttagtcca aggggaaaaa tcgtacctca ccaacggacc catcgccgac ctcttcatcg
     2041 tcttcgccgt gaccagcgaa tccgacggca ggaaggaata cacggccttt ttggtgccga
     2101 aagacacgcc gggactttcc ctgacggagc cgttgaaact ggactacctt cgcccctctc
     2161 cccattgcgg catcactgtc gactcctgcc gtgttcgggc caccgacgtc ctgggggagg
     2221 tcggctccgc ctaccgaaag atggtcatcc ctttccgcga cctggaggat gccctcctga
     2281 tgggtcccgt gatcgggggg atgaaggccc agatagattt cttggtcacc gcctacatgg
     2341 aagccacagt tcccgcgact gacggaatca aaacggagtt gggtgccctc gacttcttgg
     2401 cccgaacgat gagagtttgc tcctatcaag tggcccgcat gatcgacagc ggcatggata
     2461 cggaggcgta ccagcccttg ttgctctccc tgagatacct tgccaaggac ttccagtccc
     2521 gcgtggaacg gctcgccgga gtcacaggcc tgagcaatca aggggaattc aaggcattga
     2581 cgaccgacat tggtaagatc atcaacattg ccggtaacgt cgctctcatc aaacagaaaa
     2641 aactcggaga aagactcctc tcccagaagg gtgaagcatg aaacagaaaa aggatgaaaa
     2701 atttacctcc ataagacaca tccaggaggg tttcaaaaaa gtgggttaca tcaccagccg
     2761 gcagacggcg acgacagtct atctcgccca ccacctcgaa aagcccatcc tcgtcgaggg
     2821 acccgcaggc gttgggaaga cggaacttgc aaagactatc gcggcctatc tcgacctgcc
     2881 gatgatcagg ctccagtgtt acgaaggcct ggacgagtcg aaggccctct acgaatggaa
     2941 atacggcaag cagcttctct acacccagct cctccgggac aaactcgaca gtctcgtcga
     3001 ccgcgaggac agcttctccc agtcgatcaa caaactccac gcctacgacg acatcttctt
     3061 ttcctggcac tttctcgaac cccggccgct tctaaaggca ctgcaggaaa aagacgggac
     3121 cgtccttctc atcgacgaag tggacaaggc ggacgatgaa ttcgaggcat tcctgctcga
     3181 ggtcctgtct gattttcagg tctccatccc ggagatcggc acggtccacg cggtgacaaa
     3241 accccttgtc gtcttgacga gcaacaacac ccgggaattg agcgaggcgc tgaagagacg
     3301 ctgcctccat ctttttatcc ctttcccgga gccggcgttg gaacgcgaga taatccgggt
     3361 gcgcgtcccg gagataccga gcaatcttgc cgcccagttg gtgtctttca tccaggccgt
     3421 caggaagatg gacataaaga aagtgccctc gatcagcgag accatcgact gggcccgcgt
     3481 gctcctcatc ctccacgcgg acagcctgag cccggacctc gttcgcgaca cactccacat
     3541 ttttatcaag tacgaggaag acatccgggt cgtggaagac cagctccatc agttgaccgc
     3601 aaacgccgcc cgggcgttct gagggttaaa agaggaaggg atggacaggg tcctggaaga
     3661 tttcatcacc acgctgaggc gctcgggcgt aaggatatcc gtctcggaga gcctggacgc
     3721 tgcccgggct ctccagctcg tgagctacga cgacagaagc ctcctgaaag actcccttta
     3781 cgtcgccctg gcgaaatcgg tgcaggaaaa ggagattttt tccgactgct tcgaccgttt
     3841 cttctctttc gatttctttt ctgagccacc ccccaacgag gggtcgcaca aaggatcgac
     3901 tgagggattg tcagacctct cccggatgct catgaccggt gaccagcccg gcctcgtcac
     3961 ttacatgaaa gaggccgccc gcgcggaaaa cctctccgat atccggtacc agacgcaaaa
     4021 gagcctttac gccatgcgga ttctcgaacg tatgggcctg agcggtctcg acgccgatat
     4081 ccgggacctt tatcaggctg gtgctccttc ttcagcggcg acggcggcca ggttggagga
     4141 agcgaggagt tatcttgtcg aaaatgtccg ctcctatgtc gaggaacagc ttctcctctt
     4201 ctcaggatac gagtacgaca agatgatcga agattacttg aggaacatca agctttcgaa
     4261 cctggagcag cgttactacc accagatgca gggcatcatc cagcggatgg tcaagagact
     4321 gaacgacctc tattcccgca agcggaagcc ggcgaggagg gggcatctcg acttcaaaca
     4381 gacgattcgc gataacgtcg cttaccaggg gtttctcttc aacccgaggt ggaaaaaggt
     4441 gaagatcgaa aagccgaacg tcgtggccat ctgcgacatc agccgttccg tatcccgtct
     4501 cgtccggttc ttcctgctct tcctttacgg cctcaacaag gagatcatcc aggtcaggtc
     4561 tttcatcttt tgctccaacc tcgctgaagt taccggcatc tttgaacaat atcctatcga
     4621 ggaagccgtc gcacggatcc aggcggggac ggatattcct gttctcctgg cccggacgga
     4681 ttacgaccgc accctccgcg atttcaagga acgttacctg gacgccgtca cccacaagac
     4741 caccgttctt ttcctgggtg acgcgcgcaa caactacaac aaccctcaga cgcaaatcct
     4801 caaactgctc tccgaaagat gcaagaaact catctggctg aacccggaga cgaagccttt
     4861 ctggggcacg ggggattcgg agatgctccg ttacgcaccc tattgtcacg tcctcaaaga
     4921 gtgcaacacc ctgaatcatc ttgaaagggt tgtgcagtcg ctcctcaaga gataacggac
     4981 agtcaaaggg cctttccggc aaccaaaatt tccccgtcga agagcaacct cgaggcttta
     5041 ttgaaaaatg agccgctctc ctgctatata gggaatgtaa gcacaaagag gggggaaaga
     5101 agtgtttatg gatacggaca aagaattttt cgacccgcgc tatcgggaat tcaaagagct
     5161 taccgataga atcgccgccg aagtagggct tcctctattc tattccgaag aaaaggacgc
     5221 agtcgagagg tcccggcgaa ttttttcctc ccatcccgcg gcagaggagc tcctggccta
     5281 cctcgggaat aatgaagaat atccgggcca cggcctttat cacgtaaaaa aggtcgccct
     5341 cgacgccggc gccctcatcc tcatcgacgg agccgacgcc gttggtgtcg accgggtgga
     5401 ccgcctcgtc tttctgtccc atctggccgg actcctccac gacatcaggc gctccgaacc
     5461 gaaccacgcc cgccgcagcg ccgaggaagc gggcaggata ctcaagacct tcgacatcga
     5521 aaccctggaa agagatttca tcatccaggc aatagccaac catgaggcct tccagcccgt
     5581 tcagcccctc gatgaccctt ccgcacagat gctctccaac gccctgtacg acgccgataa
     5641 attccgctgg ggtcccgata acttcaccga aaccctctgg gcgatcatca tgtcccgtcg
     5701 tttgaaactc gaagaccttg ccgagaattt ccccggcggg cttgcggcga ccgagcgaat
     5761 ccggggcacc ttcaggaccc ggacagggaa gaaatacggg cccgacttca tcgaccgcgc
     5821 cctcgagata ggtaaccgcc tcttcgcctt cctctcatcc catgcctgaa ttaaccgggg
     5881 actttttgcg gaagataggg caaacgaaaa tggactcgga atgcacttcc aagtccattg
     5941 gaataatggt ggcggtgcag ggacttgaac cccggacact acggatatga gccgtatgct
     6001 ctaacccgct gagctacacc gccacactat atcggcatca aaatcgaaga aaaatctatc
     6061 cgaaccgata aaaaaagtca actcccttgc ggctaactca gggcgtaagc gaccacaacg
     6121 ctcctcctgg ttatccccat gtcgtaggac agcacaaggg agaggaccac atcgagtttt
     6181 ccgtcgttat cgatgtcttt gatctgataa tccgcgacgt acccttgtat cttcttcgtc
     6241 ctccagtttt ccgcaaaccc gagaccgttc cactcgaggt cataaatttc actggaggaa
     6301 aaaatcttca tgttctgaaa tagcctcccc acggtcgata tgttcttcac tactagcaga
     6361 tctttctttc cgttcttgtt gatgtcatac gccaggaggc ggatgttgac gtaggggcgc
     6421 gccttttctt ctgtggcggc tatgttcttg gatatctgcg catccgaggg taaatcaaag
     6481 aaattattgc tccccccgaa ggcctcgtcg ctcttccaga tcatctcctt gctcccgccg
     6541 aaggtaagga ttttatcgat gctctttacc gtcggttcga agacccgcag atagtcgagg
     6601 ttgtcgaggg caattatctt gttgggtccg tctttttcga ggggttcgat ggccaaccca
     6661 taaacagaca gaccctgtgg tatctccatc ctcttgcctt ccttgagtct ccctttgctc
     6721 cacacgattt cgtaaatagg attttcgaac ggtttctccg accccatcct ttgccccaac
     6781 aacgtagggg tgtcggagat gttgatgacc ctcataaacc atctcagatc cttggcaatc
     6841 gtcgtatact taccgttctt gtactggagg acgaaagaat caaggatacc ccggttgacg
     6901 ctggtgacga tgatctcttt cttgccgctg cccgtcacat cagccacatc caccgacaga
     6961 tactggtcat aagacttacc cgcgattttc tgcagaagcg tgagcgtctt tcccgacttc
     7021 cggtaaacgt agacattata ggcatcgacg gtgaccacct cgttcttccc gtccccgtcc
     7081 acatcaccga catccatccc cttgaattcg gtctcgaacc gctggctcat ccagaaacct
     7141 ttcttgtcga tggccgtctg gggggcgttg atgaattcgg ggttgatcac cgccgtgaac
     7201 gtgccctgcg gagttctcac ggcgccgatc gcttcatccg taggagcctg accggctggc
     7261 ggcggctgag gcgaagcgac ggccaaactg tcgacgggtg agggaggagg agctccgaac
     7321 gttgccggca cttgcccgag gatgtggtaa tcgatgcgct tcgcaaaatc accgatcttc
     7381 gggatgacgt cgtccatgcc ctggctttga gcgaaaacat cgaccgccga tctattggct
     7441 gcaacatcca ggagttttcc gtccagactc acgctgttgc ctatttttgt gatgctgccg
     7501 tgcacgacgt agtcggcacc catcttcttg cccagttcgt acacgtcggc aacggtcagg
     7561 ttgcccttcc ctttccgttt agcaaggaca ttctggacgt cgttttttga gaccacttct
     7621 atcttcccgg caagggatat cctcgaaatc agcatatccc agacgccgtt gcggatgtaa
     7681 tctatgtcct ccgagctgtg cacgtcaaaa ggaagaacgg ctaccctctt gaccttcgtg
     7741 tcgtctgctg ccgacacgat gcactccctg gccaccagga agactccgac catgagcgta
     7801 aggcagacca gagagattaa aattcttcgc atgttcctgc tcctttttta aaacgaccgc
     7861 gcttctgata aagcgctcgg cgatttttaa cacctctttc cacagaacac aagtcatatt
     7921 ccaagaaatt taacggcttg aagtcaataa gctttcagaa tataatgtgt cgctattgat
     7981 agtgttgtct ttccgccgcg ttcaacgttg atttcttata aagccttctt ggtcttcttc
     8041 ccccgtctcc cgggtcggga gtattagttg tgatttagga gacatgcatg ccccttgacc
     8101 attggcgttc ggtgagtccc gagaagtttg cctctgagga tgtgatcttt cgtcatatcc
     8161 atcgaggaga cacgatcttc atcggctctg cctgtgccga gccgcagtat ttggtgcagg
     8221 gtctcatcag gtacgtcaag gcgcacccca aggccttctt cgacgccgag gtcctccaca
     8281 taaggaccct cggggtagcc ccctacgcca ccgagaagtt cagagacaat ttccgtcaca
     8341 actccttctt catcggcgac agtacccggg aggccgtgaa caagggatta gccgactaca
     8401 cccccgtctt tctctcccag gtgcccgacc tattccggcg cgggctcgcc cgggtggacg
     8461 tggccctgat ccagacctct tccgtggaca tccacggcta catgagcctc ggggtgagcg
     8521 tagacatcgt caaggccgcc gtcgagacgg cctccgtcgt catcgcccag gtcaactccc
     8581 gtatgcccag ggtccacggc gacgccttca tccacatcga cgacatcgac ttcgtcgtcc
     8641 cccgcgacga agagatactg gagtacagcc ccgaggcgga caccgaggca gcccagagga
     8701 tcggcaagta cgtcgcccgc ctgatcgagg acggcgacac gatccaggta ggctacggca
     8761 gcatccccaa cgccatcctg gccaacctct cggagaagaa gcacctgggg gtccacaccg
     8821 agctcataag cgacggcgtc gccgacctga tgaagaaggg agtcatcgac aacacccgca
     8881 agaacatcaa ccggggcaag accgtggcca ccttctgcat gggcaggaga gccacctacg
     8941 actacatcca cgacaacccc gccgtagagt tccgcacaat cgactacacc aacgaccccc
     9001 tcgtcatcgc ccagcacgac ggcatggtgg ccatcaacag cgccctcgag atcgacctca
     9061 ccggccaggc cacggccgag tccatcggcc ggacgttcca cagcggcgtc ggcggtcagg
     9121 ccgacttcat gaggggcgcg gtcctctccc ggggcggcaa gaccatcctg gccctcaagt
     9181 ccacggccgc ctccgacgtc gtctcccgca tcgtccccgc cctccgggaa ggggccggca
     9241 ccaccctcat ccgcggcgac atccattacg tggtcaccga gtacggcatc gcctacctgc
     9301 acggcaagaa catcagggaa agggccatgg aactcatcgc tatcgcccac cccaagttcc
     9361 gccccgggct catatccgag gccaagaagg cgggcctcat ctaccaagac caggccttca
     9421 tccccggcga aaggggtatc taccccgaag aactcgagac atggcggact accaagacgg
     9481 gcctcaacat cttcttgaga ccgataaaga taagcgacga gccgctcctg aaagacttcc
     9541 tctactccct ctccgataaa agcctctacc gccgcttcat gtcccagaga ttccacatgc
     9601 cccacgagtt cctccaagaa ctggtcgtga tcgactacac gagggagatg gctatcctgg
     9661 ctgtcgtggg agaaaccccg aacgaaaagg tcgtcggcgt gggccgctac ttcatcgacc
     9721 cggaaaccca tacggccgag gtcgctttcg ccgtccgaga cgacttccac aaccagggca
     9781 taggctcaga actgctgagc tacctcactt acctcgctaa aagacagggc ctcctgggct
     9841 tcacggccga ggtgttcgcc gataacgccc ccatgctcca tgtcttcgaa aaaggagggt
     9901 tcgatatcca gaaaaggacc atcgccgggc tctgcgaact gaagatgact ttc
//
LOCUS       DAOTMX010000018         9932 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_6318_length_9932_cov_4.057406, whole genome shotgun sequence.
ACCESSION   DAOTMX010000018 DAOTMX010000000
VERSION     DAOTMX010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 9932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9932
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6318_length_9932_cov_4.057406"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="P5573_01040"
     CDS             <1..340
                     /locus_tag="P5573_01040"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HRT26684.1"
                     /translation="VMVGLGIAMILVCLLAVWFSRKEDIAGRTWLLKLALLSLPLPYV
                     AGLLGWVVAEVGRQPWIVYGVLKTADAVSKSVSTAQVAGSLAGFTLLYGGLAVVDVWL
                     LARYARRGPA"
     gene            386..1414
                     /gene="cydB"
                     /locus_tag="P5573_01045"
     CDS             386..1414
                     /gene="cydB"
                     /locus_tag="P5573_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HRT26685.1"
                     /translation="MELQVLWFALWALLWTVYFILDGFVLGLGMLFGRLSRSETERRI
                     LLRSVGPVWNGNEVWLVTAGGATFAAFPTTYALMFSYFYTALLLLLFSLIVRGVAIEF
                     RAKVDTTAWRTTCDGAIAVASFLPAFLFGVAFGNIFRGLPMDAVGYHGSFLGLLNPYG
                     LLTGVLFVLLFAHHGALYASVKTVGALSDRARQAARRLWPWVLVVALAFLAASKPATR
                     LSDNYLHWPVLAVIPLIAVAALAAIRFFESKGSLPAAFAASCVTILFVTFTGVAGLFP
                     SLIPSSIDPAYSLTIYNSSSSPYTLAVMTVAALIFVPIVIAYKVWVYRVFSAPVEEKD
                     VLTDTGMY"
     gene            complement(1642..2175)
                     /locus_tag="P5573_01050"
     CDS             complement(1642..2175)
                     /locus_tag="P5573_01050"
                     /inference="COORDINATES: protein motif:HMM:TIGR03830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system MqsA family
                     antitoxin"
                     /protein_id="HRT26686.1"
                     /translation="MNTCIHCKEACQVIKGKPYHYTECGLDNVYIHGVNQYRCPKCGE
                     TAVEIPKVKDLHLLIGKDIVCKDGLLEGQEIRYLRKEIRLRSGEMAELLSVDAATYSR
                     WENSKKPIGEAEDKLLRLIYILNAENETGRLLHKGIRALNVLKRYAPSKKKKEKIELT
                     VADWMMLNKPLFGESGC"
     gene            complement(2193..2495)
                     /locus_tag="P5573_01055"
     CDS             complement(2193..2495)
                     /locus_tag="P5573_01055"
                     /inference="COORDINATES: protein motif:HMM:NF025442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HRT26687.1"
                     /translation="MNRQSADEILDVIRGILTKSGSITIPGHAKRRMEERNYTVQDVF
                     NIIEKGSLGPLQTSEKPGEERYEIIGNDLDGHPGAVIVEISPDRNKIILITVKGGV"
     assembly_gap    3615..3624
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3625..5879
                     /locus_tag="P5573_01060"
     CDS             <3625..5879
                     /locus_tag="P5573_01060"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HRT26688.1"
                     /translation="ILLLSLCFLMSCSFSKDIQISRYAQKQQIHKIIDVLQPSIDAGE
                     EVSAFDLYFLSASYISVRNYSKALATIDLMQKRIDAGDKSYVGSDLTVYPQILRGTVY
                     LEQGEPQKAIHEGELAYKLLHEGGRDRAGFYTSQLIDIYNLLGVAYAVSGESAKGRNT
                     ARKLEGVSADAINGAAKYIALARIYMALKKYDLALEAIRNPAAKVSGFITAFFDQTFQ
                     EIPNLFVLTKSLYETGRIKEAREGYDQLLRHPQIAQIGGIYWIVLLDRSRIALSDGQR
                     DIAEDLLKKAIDVIETSRSSITSEAGRIGFVGDKQTAYSDMTKLLLAAGRYAEAFSYV
                     ERSKARALVDLLASQKNIGSRGLKKERVETTLAELDKAERDLAVVAPIDDQGSQSKTR
                     SIALALKKDLQEKAPELASLVSVTPPSTADIQGRLQNDETLLEYYCSGQDWYLFVVTP
                     KVVTAQRIPAADLEGRIDMLRKHIAERNNAGFEEESRALYRQLFAPAMPSIKTTKVIV
                     VPHGPLHYLPFAALSDGKGYLVDRFSIRMLPSASVLVFLKSPAKQGEQNALIFGNPNL
                     NDERYDLKFAQDEALAIGRILPNSRVLLRGEASKANLLAWGERYSLVHLAVHGVFDQE
                     KPLDSALLLAADGKGSGLLRVGDIYQLSLKADLVSLSACETALGKVATGDDVVGFTRG
                     FLYAGARSLLSSLWQVDDKATHDLMVAFYSNLSKMDKDEALRQAQIKTKRQYAHPYYW
                     AAFLITGSAR"
     gene            complement(6140..6502)
                     /locus_tag="P5573_01065"
     CDS             complement(6140..6502)
                     /locus_tag="P5573_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT26689.1"
                     /translation="MTEFVDYISTWSVLKWVVLVLAAGFIGHFGRMMAEAIIARARVR
                     RSRKGERPHEEDTVTTNKLDDRTVEITDREGAAAKPPSAESNRFAAAGPQPLDKKMLK
                     TIAKARKKEAKSRLKQNK"
     gene            6849..7613
                     /gene="hisF"
                     /locus_tag="P5573_01070"
     CDS             6849..7613
                     /gene="hisF"
                     /locus_tag="P5573_01070"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HRT26690.1"
                     /translation="MDVVKIMPCLDMKNGRVVKGIHFVDLKDAGDPVENAAFYQHEGA
                     DELAMLDIAATLENRKTRLEWVKGVAAVITIPLTVGGGISSLEDIELLLAAGASKISM
                     NSAAVKRPELVSEAVARFGSERITVAVDARRNKAMPSGFELVVSGGTLPTGKDAIRWA
                     KECEARGAGVILPTSMDSDGTQAGYDLEFLRAISGAVGIPVVASGGAGKLEDFYRAVK
                     EGGASILLAASVLHYRILSIRQIKEYLRERGIPVSL"
     gene            complement(7646..8653)
                     /locus_tag="P5573_01075"
     CDS             complement(7646..8653)
                     /locus_tag="P5573_01075"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRT26691.1"
                     /translation="MKENPILETRGLAIGYRHHRRPVCVVAEGIDVALSAGHFVCLLG
                     PNGAGKSTLIRTLAGMQAPIEGTIFIAGKHLHELSARALAQRMSLVLTQRVAVGMMPV
                     LSFVALGRHPYTDWMGRLSREDEAAVSRALADVGIEALAHRAVCELSDGERQKVKIAR
                     ALAQEPQVMILDEATAFLDLPRRVELMSLLKRLAHESGRAVLLATHDLDLALRSADRL
                     WLLPSGGPLREGVPEDLVLDDAFSATFSDGAIDFDKESGAFVVVGRGKGTVSLHGDGT
                     PAMWTYRALERAGYRVVSNGVAGISVQVLLENGRPVWRLCGEEGLLFSSVEEMLAALD
                     E"
     gene            complement(8650..9648)
                     /locus_tag="P5573_01080"
     CDS             complement(8650..9648)
                     /locus_tag="P5573_01080"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRT26692.1"
                     /translation="MAAAFTLELSLGSVWIPLKAVVSILLGQADTTEDWRQIVLLFRL
                     PRALTGIFAGAALGIAGLKMQTLFRNPLADPFVLGISSGAGLGVALVVMAASGLKWNI
                     FLERAGLAGNISLTLSAILGATAVLGIVLSVARRVESNLTLLIVGLMFGYITGAAVQV
                     LMQFSLEHQMQSYITWTFGSFGSVTWEQMGVFAPTVLCGLAVAWALVKPLDALLLGEG
                     YARSMGVNVRLVRSWIIGGASVLAGAVTAYCGPIGFLGIAVPHLGRVLLKTSEHRRLV
                     PAVMILGATLALLADLISQAPGTQMALPLNAVTSLIGAPVVIVVILRRRHTMEADG"
BASE COUNT         2037 a         2824 c         2851 g         2210 t
ORIGIN      
        1 ggtcatggtg ggcctgggaa tagccatgat cctggtttgc ctgttggccg tctggttctc
       61 ccgtaaggag gacatcgcgg gtcggacatg gctcctgaag ctggccctcc tgagcctgcc
      121 cctgccttac gtcgccggcc tcctcgggtg ggtggtcgcc gaggtgggac ggcagccctg
      181 gatcgtctat ggagtcctca agaccgccga cgccgtctcg aaatcggtca gcacagccca
      241 ggtcgcgggc tcgctcgcgg gcttcacctt gctctacggg gggctcgcgg tggtggacgt
      301 atggctcctg gccaggtatg cccgcagggg accggcttaa cccgaatgcc tgccggcgca
      361 gcagccggga gaggaggaga tgacgatgga attgcaagta ctctggttcg cgttgtgggc
      421 cctgctgtgg actgtttact tcatactcga cgggttcgtc ctggggctgg gcatgttatt
      481 cggtcggcta agtcggagcg agacggagcg acggatactg ctgcgctccg tcgggcccgt
      541 ctggaacggc aacgaggtgt ggctcgtgac cgccgggggc gccaccttcg cggccttccc
      601 gaccacttac gcccttatgt tcagctactt ctacacggcg ctattgcttc tgctcttttc
      661 ccttatcgtc cggggcgtgg ccatcgaatt ccgggccaag gtcgacacca cggcctggcg
      721 gacgacctgt gacggggcca tcgcggtcgc gagcttccta ccggcgttcc tgttcggcgt
      781 ggccttcggc aacatcttcc ggggtctgcc gatggatgcg gtgggatacc atggatcgtt
      841 cctgggcctg ctgaaccctt acggcctttt gaccggggtt ctctttgtcc tcctctttgc
      901 ccaccacggg gctctttacg cctcagtgaa gacggtcggg gccttgagcg accgggcgcg
      961 acaggcggcc cgccgcctct ggccatgggt tctggtcgtg gctctggcct tccttgcagc
     1021 ctcgaagccc gccacacggc tctccgacaa ttacctccac tggccggtgc tggcggtgat
     1081 ccccctgatc gccgtggcgg ccctggcggc catccggttc tttgaatcca aagggtcgct
     1141 gccggcggcc tttgcggctt cctgtgtcac gatcctcttt gtcaccttca cgggggttgc
     1201 ggggctcttc cccagcctga tcccctccag catcgacccg gcctacagcc tgacgatcta
     1261 caactcctcg tccagcccgt acaccctggc ggtgatgacc gtggcggcgc ttatcttcgt
     1321 gcccatcgtg atcgcctaca aggtctgggt ctaccgggtg ttcagcgccc cggtcgagga
     1381 gaaagatgtc ttgaccgaca cgggaatgta ttgaatggcg tatagctcga agggtttttg
     1441 ggacgctttc ctcgaaaaga acggctgcga attcctgcgg gagggcggcg accacaccgt
     1501 ttatgttgat cgaaaagata agaaagtaag gctgttaacc caagctacct gataataggc
     1561 ccatgaagca gtgaaaatgc ctgatcgggc aagcaatgct tgcctatctc aatgacgcca
     1621 tactgcaatg ctctccggct gtcagcatcc gctttcaccg aacaggggtt tattcagcat
     1681 catccagtcg gcgaccgtaa gttcaatctt ctcttttttc ttcttggaag gtgcgtacct
     1741 ttttaaaacg ttcaaggccc ggatgccctt gtgaagaagg cgcccggttt cgttctctgc
     1801 gttcaggata taaatgagcc gtaggagctt gtcctccgcc tcccctatgg gtttcttgga
     1861 attttcccac ctcgaataag tcgcggcgtc cacagacagg agctccgcca tttctccgct
     1921 tctcaaccgt atttcttttc tgagataccg aatctcctgc ccctcgagca gcccgtcttt
     1981 gcagacgata tcctttccga tcaaaagatg gagatccttg accttaggga tttcaaccgc
     2041 cgtctcaccg catttgggac acctgtactg gttgacccca tgaatgtata cattatccaa
     2101 gccgcactcc gtgtaatgat acggcttacc cttaatgacc tgacacgcct ccttgcagtg
     2161 aatacaagta ttcacagtcc ctctcctttc ctctacacgc cgcctttcac cgtgatcagg
     2221 atgatcttat tccgatccgg agaaatctcc acgatgactg ccccgggatg gccgtcgaga
     2281 tcgttcccga tgatctcgta tctttcctcg ccaggcttct cagatgtctg gagaggacca
     2341 agggaaccct tttcgatgat gttaaacaca tcctggacag tgtaatttct ttcttccatt
     2401 cgccttttgg cgtgtcctgg gatcgtgatg gacccgcttt tggtgagaat acctctgatg
     2461 acatcaagaa tttcatcggc agattgcctg ttcatgtata gtgcttaact tacttttggc
     2521 ttcggagtca atataattga ttttaagtca taatgtggag tcatctatcg cgctcggcaa
     2581 agatattgtt gatcttggat catgtatgaa aacttaacaa attgatggaa tgaccagagt
     2641 ttttcgccgg atatccattt gtaggcgaac gtgctcatcc gtccctgatg gcccattcag
     2701 cgccgaacta caggaatcgg agtaccttgg gatagacagg tgacgccgta ggtgtgcgaa
     2761 agggtccggt tgtccccgtg cacgtatgga ccatctttcc tgtagttggg gaagtttgac
     2821 gttcccgaat ccatgcggca agttcgatga ggtcggcaat atcgaagatt ctccatgaaa
     2881 gactatcctt ttgtcgggaa aatatataga cagcaccaac ggcctgacca attcgagccg
     2941 taagcttttg tttccataca tgtatttttt tttgatacca tgaacttggg ttaaaacgcc
     3001 ggaaaggaga tatgtatgcc atcgttccgc agccacaacg tactcgccgc tctccgtttg
     3061 atgtcgcggg attgaataag gggatgagca gcgaagaaat agtggctgca attcaagaat
     3121 cgagagaaag ggtataacga gtcgttccat cggatcgacg gaaaaagacg cccccgggac
     3181 tcttcgttga gcatcaaccg ccgcccaaca gcatcaacgg cgcgaacacg gacgacggct
     3241 ttttacgatt cggtcgcctt gcgtcattcc ggcgaacgtc ggaatccagt atgctcaata
     3301 tgttatggat gcccctgtat ttaggacttt gactttgtga ttaaatcgcc ttactgtcat
     3361 ttcgtgcttg actttaacct gccccgtact cggggcggcg gaatccaatg ttttcaatct
     3421 gttctggatt ccgccccgat tttcattggg gtgacgggtc ggaatgacat gaaggagtgt
     3481 tttcaggtgt ctcgtattga gtacggggca ggtttaatcg atttcggcat gacgttttta
     3541 gattttttac gagggcgtcc cctatcctgt cccagcagct gacaggtggc tctcttaacc
     3601 aagccaacgg gagannnnnn nnnnggattc tattgctctc tttatgcttt ttgatgtcct
     3661 gctctttttc gaaggacatc cagatcagcc gctatgccca gaagcagcag attcacaaga
     3721 tcatagatgt gcttcagccg agtatcgatg cgggcgagga agtttccgcc ttcgatctct
     3781 attttctgtc cgcctcctat atcagtgtcc gaaactacag caaggcgctg gcgaccatcg
     3841 atttgatgca gaagcggatt gatgcgggag acaaatccta tgtaggttcc gatctgaccg
     3901 tctatcctca gatccttcgg ggtacggtct acctggagca gggtgagccg cagaaagcga
     3961 tccacgaagg ggaactggct tataagctgt tgcacgaagg cggccgtgac cgagcgggct
     4021 tctacacctc ccagctcatc gatatctaca acctccttgg cgttgcctat gccgtttccg
     4081 gagaatcggc caaggggcga aacactgccc ggaagctgga aggtgtctcc gctgacgcaa
     4141 tcaacggtgc cgccaagtac attgcgctgg cccggatcta catggccctg aagaaatacg
     4201 atcttgccct ggaggccatc cggaatccgg ccgctaaagt ttcagggttc atcaccgcct
     4261 ttttcgatca gacctttcaa gagatcccga atctatttgt cctgacgaag agtctctatg
     4321 aaaccggcag aatcaaagag gccagggaag gatatgacca gcttctgcgc catccgcaga
     4381 tcgcacagat cggaggcatc tactggatcg tcctgctaga caggtccagg atcgctttgt
     4441 ccgacggaca gcgggacatt gcggaggacc tgctcaaaaa ggcgatcgac gtgatcgaga
     4501 cctcgcgctc ttcgatcacc tccgaggccg gacgcatcgg ttttgtcgga gacaagcaga
     4561 ccgcgtattc cgacatgacg aagctcttac ttgcggccgg ccggtacgcc gaggcctttt
     4621 cctatgtgga gcgctccaaa gcccgcgccc tggtggactt gcttgccagt cagaagaata
     4681 tcggttcccg tggcctcaag aaggagaggg tcgagacaac acttgccgaa ctcgacaagg
     4741 cggaacgcga tctggccgtg gtcgccccaa tcgatgatca gggaagccaa agcaagaccc
     4801 gttcaattgc cttagccttg aagaaggatc tgcaggagaa agctcccgag ttggcctctc
     4861 ttgtttccgt tacgcctccc tcgacagccg acattcaagg caggcttcag aatgacgaga
     4921 ccctgcttga atactactgc tccgggcaag actggtatct ctttgtggtc acccccaagg
     4981 tggttacggc gcaaaggatt ccggcagcgg acctggaagg gcgtatcgat atgttgcgca
     5041 agcatattgc ggaacgcaac aatgcgggat tcgaggaaga gagcagagct ctgtaccgcc
     5101 aactctttgc gccggcgatg ccctcgatca aaacaaccaa agtgattgta gttccgcacg
     5161 ggcctctgca ctatctcccc tttgccgctc tttcggacgg caaaggatat ctggttgacc
     5221 gcttcagcat ccgcatgctt cccagcgcca gcgtgctggt ttttctcaag tcacctgcaa
     5281 agcaagggga gcagaacgct ctgatcttcg gcaatcctaa tctcaatgat gaaagatatg
     5341 atttaaaatt tgcgcaggac gaagccctgg cgatcggtcg aattcttccc aactccaggg
     5401 tgctcctgcg cggagaggct tccaaggcca acctgctcgc gtggggagag agatattccc
     5461 ttgttcattt ggcggtccat ggcgtcttcg atcaggagaa acctctcgat tcagcactgt
     5521 tgctggcggc cgatggaaaa gggagcggtc tgttgagggt cggtgacatt tatcaactct
     5581 cattgaaggc cgacctggtc agcctgagcg cctgtgagac agcccttgga aaggttgcga
     5641 caggcgatga tgtcgtcgga ttcacgcgtg gtttcctcta tgccggagcg cgctcgcttc
     5701 tatccagcct atggcaggta gacgacaagg ccacccatga cttgatggtt gccttctaca
     5761 gcaatttatc caagatggac aaggacgagg ccttgaggca ggcgcagatc aaaacgaagc
     5821 ggcagtatgc ccatccttat tactgggcgg cgtttcttat tacggggagt gcaagataag
     5881 gcgagaagca gaggcgttag gatgaatacg ggacgccctt tacgtgttta catgtgcatg
     5941 acgagaaagg gacgatcagg tcattaatgc caaggtaagc cggagtaagg atgtgtattt
     6001 gacgagctcc taaaaataaa gatgtaaagg gcgtcccgta ttccaccgaa gcggccgaga
     6061 agggactcaa tggaagttat aaacgtgtct ggaaagaacg agggccgata cggcctggca
     6121 tggcggtcgg gtggcgacgt tatttgttct gtttcagtct gctttttgcc tcttttttcc
     6181 gggctttggc gatagttttc agcatttttt tgtccagggg ctgtgggccg gcagccgcaa
     6241 agcggtttga ttcggcagag ggcggcttgg cagcggcgcc ttcacgatcg gttatctcga
     6301 cggtcctatc atcgagttta tttgtcgtta ccgtgtcttc ttcatgcggt ctttccccct
     6361 tgcggcttct acgcaccctg gccctggcta tgatggcttc ggccatcatc cgcccgaaat
     6421 ggccgataaa accggcggcg agaacaagca ccacccactt gagcacggac caggtgctga
     6481 tataatccac gaattccgtc aaaagataac accccgatat aatcgtgaaa tggcaccgtt
     6541 caccaaggaa cgctcttcag gggccgcctt tttttaccgc ataaagagcg acgatggcaa
     6601 ggcctattct cccatcaaca ctgcaggagt cgttctcttc cagaagttcc gtatcagagt
     6661 aagcaggctt ggtcgatccc ggcagagcgt ttttcggttt tcacgaatga ttccattaag
     6721 ttcaggtttg tttcctacct tcgggccggc gaggcctggt tccggtggtg ctttctcttg
     6781 acaccccgct cgtttttcta ttatggtctc ccgcctgaaa cacgaaagac ctgaaggggg
     6841 aggatgccat ggacgtcgta aagatcatgc cgtgcctgga tatgaagaac ggtcgggtgg
     6901 tgaaggggat tcatttcgtc gacctgaaag acgccggtga cccggtggag aacgccgcat
     6961 tctaccagca tgaaggggcc gatgaactcg cgatgctcga catcgcggcc accctcgaga
     7021 accgtaagac ccggctcgaa tgggtgaagg gtgtcgccgc cgtcatcacg ataccgctca
     7081 ccgtgggtgg gggcatctcg tccctggagg acatcgaact gctcctggcg gcgggggcta
     7141 gcaagatctc catgaacagc gcggccgtca agcgcccgga gctggtgagc gaggccgttg
     7201 cccggttcgg cagtgagcgc atcaccgtgg ccgttgacgc aaggcgcaac aaggccatgc
     7261 cgtcggggtt cgagctcgtc gtttccgggg gtacacttcc gaccggcaag gatgccatcc
     7321 ggtgggcgaa ggaatgtgag gcgcgggggg ccggagtgat cctgccgacg agcatggaca
     7381 gcgacgggac gcaggcaggc tacgaccttg aattcctccg ggctatctct ggcgccgttg
     7441 ggatccccgt ggtcgcctcg ggcggcgcgg ggaagctgga ggatttttac cgcgccgtga
     7501 aggaaggggg cgcaagcatc ctgctcgccg cgtcggtctt gcattatcgc atcctcagca
     7561 tccggcagat caaggaatac ctgcgagaaa ggggcatccc cgtttctctg tagattccgc
     7621 cttcctcatt ttgcttcttg tgtagttatt catccagggc cgccagcatt tcctcgacgg
     7681 acgaaaagag aagcccttct tcgccgcaga ggcgccagac gggcctcccg ttctccagca
     7741 gtacctgtac ggagataccg gctaccccgt ttgaaaccac gcgatatccc gcccgttcca
     7801 gggcccggta ggtccacata gccggcgttc cgtccccatg gagcgacacc gtgccttttc
     7861 cccggcccac aaccacaaag gcgccggatt ccttatcgaa gtcgatggcc ccgtcggaga
     7921 aggtcgcgga aaaggcatca tccagaacga ggtcttcggg gacgccttct cgtaggggtc
     7981 cacccgacgg cagcagccag aggcggtccg ccgagcgcag ggcaagatcg agatcgtggg
     8041 tggcaaggag tacggcccgg ccgctctcgt gggcgaggcg tttgagcagg ctcatcaact
     8101 ccacccggcg cggtaggtcg aggaaggccg tcgcctcgtc gaggatcatc acctggggct
     8161 cctgcgccaa agccctggcg atcttcactt tctgcctctc cccgtcgctc agttcgcaga
     8221 ccgccctgtg agcgagcgcc tcgatgccca catccgccaa ggcccgtgat accgccgcct
     8281 cgtcttcccg gctcaaccgg cccatccagt cggtatacgg atggcgcccc agggccacga
     8341 aagacaagac gggcatcatg cccacggcga cgcgttgcgt caggacaagg ctcatccgct
     8401 gcgccagcgc gcgtgccgaa agctcgtgca ggtgcttacc ggcgatgaag atcgtcccct
     8461 cgatcggcgc ctgcattccg gcaagggtcc gaataagggt tgacttcccc gcgccgttgg
     8521 gtcccagcag gcagacgaaa tgccccgctg aaagggcgac gtcgatgcct tcggcgacga
     8581 cgcacaccgg gcgcctgtgg tgccggtagc cgatggcaag tcctctcgtt tccagaatcg
     8641 ggttctcttt catccgtccg cctccatcgt gtgcctgcgc cgcagtatca cgacgatcac
     8701 gacgggcgcg cctatcagag acgtcacggc attcagggga agggccatct gggtgccggg
     8761 cgcctgagaa ataagatcag caaggagcgc cagcgtcgcc ccgaggatca tgaccgcggg
     8821 aacgagccgg cgatgttccg atgtcttgag gaggacccgg cccaggtggg gcacggcgat
     8881 cccgaggaaa ccgatcggcc cgcagtaagc cgtgacggcg cccgcgagca ccgaggcgcc
     8941 gccgatgatc caggaacgca cgaggcgcac attcaccccc atgctgcgtg catacccctc
     9001 tccgaggagc agcgcgtcaa ggggtttgac aagcgcccag gctacggcaa ggccgcagag
     9061 gacggtgggc gcaaagacac ccatctgttc ccaggtgacg ctgccgaagg agccgaaggt
     9121 ccaggtgata tagctctgca tctgatgctc cagggaaaac tgcatcagta cctgaacggc
     9181 tgcgccggtg atgtatccga acataagacc gacgatcagc agggtgagat tgctctccac
     9241 ccggcgggcc acgctcaaga caatgccgag caccgccgtt gcgccgagta ttgcggacag
     9301 tgtgagggag atattgccgg ccaagcctgc ccgttccagg aagatgttcc acttgagacc
     9361 gctggcggcc atgaccacca gggcgacgcc gagaccggcg cctgaactga tcccaaggac
     9421 aaaagggtcc gccagaggat tgcggaagag ggtctgcatc ttgagacccg caataccaag
     9481 ggcagctccg gcgaagatgc cggtcaaggc tctggggagc cggaagagaa gcacgatctg
     9541 ccgccagtct tccgtcgtgt cggcctgacc gagaagaatc gacacgacag ccttcagggg
     9601 aatccaaacc gatccgaggg atagctccag agtgaaagcc gcggccatcg ctaaaaacat
     9661 gcctccccac acgaagggtc ccgtgcgcgg caggaaaggc tgtggcagcg tccccggtaa
     9721 ggttgatgat atatgcgttt cgctccggct ttttgtcata gaattccttt caggggtttc
     9781 ccaaaatcga cgtgggtgat atttcctgct tcgaaacgat gccttttccg ttatgaaagg
     9841 ccttgctcat tttccccgtg ctctgaagcc atcagtgccg tccggcatgt tcggcggcag
     9901 gtgtaagtgc cagatcagct tatgccccgg aa
//
LOCUS       DAOTMX010000019         9785 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_6411_length_9785_cov_3.590750, whole genome shotgun sequence.
ACCESSION   DAOTMX010000019 DAOTMX010000000
VERSION     DAOTMX010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 9785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9785
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6411_length_9785_cov_3.590750"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="P5573_01085"
     CDS             <1..471
                     /locus_tag="P5573_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT26693.1"
                     /translation="PEEAGKFKRVFFLDPFIAGDRLEHAMIFYDIVKRNRIQCYLANT
                     GTIGQQEEKVTLRQSLAAYNDLVRMQLRFSREPDAMGYYYPIKCDRANLDMMTAFQLF
                     QDRDIFKNRVVDFFKGRQKFLEDFEGKYGKIPANIRESLPYSYKNASPREMPVE"
     gene            complement(520..1041)
                     /locus_tag="P5573_01090"
     CDS             complement(520..1041)
                     /locus_tag="P5573_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT26694.1"
                     /translation="MIDSLPGRIGTTLGRSVFIVLFVFSLLGGPGSESHGADWKPVGK
                     DTNDCQWFYDGTTVRYLPERKALVWLKRMVDDKQREIAVRERKKDGLPIEGYDRWAYE
                     LGLMELDCKEVTIRQLSVSDYDRNEKLLKSRAIGENVNGVTRDSIGEAVLNLICGSPV
                     RDIEEITPRFPAF"
     gene            1213..1551
                     /locus_tag="P5573_01095"
     CDS             1213..1551
                     /locus_tag="P5573_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT26695.1"
                     /translation="MGTINLEDIQPGMILEEAVEDRSGRILLGAGEEVTERHIRIFKM
                     WGVTEAAVKNVGEEDVSAKTAASVDPVLLRETQNTLRESFRHTDMGHAFNKEFLRLLS
                     LRMAAREGRR"
     gene            1548..2414
                     /locus_tag="P5573_01100"
     CDS             1548..2414
                     /locus_tag="P5573_01100"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HRT26696.1"
                     /translation="MRTTDGLDLERLFRDDVQVPSLPIIYRRINDAVNNPRCSTSDIS
                     AIIGDDPGLTSRLLRLVNSAFYGYPSGIGTISRALLIVGTHQLRDLALGTSIIELFKG
                     ISRTLVDMESFCRHSIACGLAAKILGTYCQTQSTAESFFVAGLIHDIGRLLIYKKRPE
                     SAGKALALARDKGELLHKSERAVFGFNHTDVGRLLIRRWNLPPSLEEVAAYHHNPTSA
                     VFYPMESAVVHVADIIANGMQLGSSGECLVPPLNERAWELLGLRVSVLSPVMDQLERE
                     AAEVQQLIFENT"
     gene            2417..4627
                     /locus_tag="P5573_01105"
     CDS             2417..4627
                     /locus_tag="P5573_01105"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRT26697.1"
                     /translation="MNDRLLKLEQRVQYLEELNRSTLRALDLAMSFCDCQNRLSLPHL
                     DNRIIFNSACSYLKRIIPFRAMAFYLTDEEDPEFRLKSCNPFAERPTIEEDVDRQVSN
                     GVFAWALRQNRAVIIPSSKPDRKVVLHSLVTASQTIGMFVGILDDGEIKVDKNIAYLL
                     TIIFFNTARSLENAGLFKKVRDHNLDLERTVKRRTAELNAALENAEAANVAKSRFLAN
                     ISHEIRTPMSAVIGFTELLAKTELNREQSDYANMIKLNSEILLDLINDILDFSKVEAR
                     RLELECADFNPELTAYEVCEVLLTRLAHKPVTLSCRIGDGLPLFVCGDRHRFRQVLLN
                     LAHNAVKFTDSGEIVVSLDKEDERDGAVKIHVSVSDTGVGVPEEKLQVIFEPFRQADG
                     SSTRRYGGTGLGLAICKQLAELMEGDTWAESSPGKGSTFHFTAWMKRSKQDRVRDFYH
                     PALAGKRVLLIDESKSHLSSLAHLLESAGMRPTTATEVGFILESLKGAIEEKDPYEFI
                     IVNVKTPGLRTDGFAEKVRAAGGPLYLVAVGSAVRPSAEKYKKAGFNLFVRMPARRDK
                     FVRDLANLVESHDEEGRKGEDKTVRPCMRKGPRILLAEDNPAGRKLMNIILNKEGCRV
                     ETATNGREAVDKFLHARGAFDLIFMDIQMPVVDGVAAARAIRERGFREIPIIALTASA
                     VDEDRRSCEEAGMNDYLIKPVARERLIEVIDRWTNRGRNHGPQEIGREARAGRG"
     gene            4581..4901
                     /locus_tag="P5573_01110"
     CDS             4581..4901
                     /locus_tag="P5573_01110"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HRT26698.1"
                     /translation="MDLRRLAERLGLGEAEFKEVVRLFIESSIADMESFTSAVRTGDP
                     AGASAAIHSIKGAALCLGLNDISRAAREIELRSRQGRLDGLAEATAAIKREIEKIAGY
                     AGER"
     gene            4921..6885
                     /locus_tag="P5573_01115"
     CDS             4921..6885
                     /locus_tag="P5573_01115"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRT26699.1"
                     /translation="MHNAIKDRKLHETRPVNKVLVADNNPVMLTLLHNILAKNGCEVV
                     TAEDGLTVLEILASYKPDVIFIDLIMPNIDGERLCRIIRSMPHMNDVVLVIISAVAAE
                     TKIPFTEWGANACIAKGPFDKMSRSIKDLLERIETDTFFPAGWRVLGPDYARPREITE
                     ELLFLKSHLETLLSSISEGIIEITPEGRVVYVNPEAVEITGGSEEKILATDITALFKE
                     EEYPKVRKALMSARANYRTAEESVLANGKEVSFTVVPVKNDRNKLVILSDLGLRKHLE
                     AELRQAQKLEAIGTLAGGIAHDFNNLLMEIQGRTSLMLLKAGENDFLKQNLKVIEDIV
                     MSGANLTRQLLEFAQNGKYVVEPTNLNDLVQRSVKMFGRTKKEIVLDLDLEENLWAVE
                     VDRGQIEQALLNLYINAWQAMPGGGTIYLKTENVRMNDKEADIRRMKPGRYVKLTVRD
                     TGTGMDEQTRQRIFEPFFTTKEKGLGTGMGLASVYGIVKGHNGSIEVKSERGEGTTFT
                     IYLPATGKKVPHERKAEEKILPGKETILLVDDEEVVTNVVREMLESLGYTVIAALNGK
                     DALELYRQNKDRIDLVILDMIMPQMSGYEVFEALKALNPKVRVILATGYSMKEQAAKI
                     MESGCRAFMQKPFSIQDLSNKIRTVLDAAE"
     gene            7015..8016
                     /locus_tag="P5573_01120"
     CDS             7015..8016
                     /locus_tag="P5573_01120"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HRT26700.1"
                     /translation="MLENAQKRKKRAAALSVASNTVLIVMKIVVGIAIGSVSVISEAI
                     HSSVDLLASAIALFSVSTSDKPADKEHPFGHGKIENISGTVEAILIFLAAAWIVYEAA
                     QKLIHPRPIDTALLGVGVMFVSAVTNAVLSHYLLTVGRETDSIALQADAWHLRTDVYT
                     SVGVMLSLAFIWLGERVSPGLDLNWLDPVCAVAVALLITRAAYQLTIQSARDLLDAHL
                     PPKEENWIRNLIEANRGVIHGYHYLRTRKAGRFRFVEFHIQVDPKMTVELSHRLAQRL
                     TREIKNRLPDTTVTVHIEPCLGDCSEKCLSGCLLTESERLRAFGHRGKIVQPAQVLK"
     gene            8043..9740
                     /locus_tag="P5573_01125"
     CDS             8043..9740
                     /locus_tag="P5573_01125"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRT26701.1"
                     /translation="MKIIYLADVHGDFPRVKDLLAVTDAQVYIVAGDLIDRPFFTEEM
                     AARYRELQSYFFRLQCRMGDGGTDMDDFVEDLLRQTDLPNDILANAREYQEKTVRARR
                     VLQQKYKVLETILSLKRNSLIFCLPGNYDMDLQYTSLHSRDLHRHWYYVADLRIAGYG
                     GADVATPGIPHRYAVPYNADRGMSEMYRFFKETRPDIVVTHKPAHGVHDRVLPMGESG
                     SLELRRFCEENHVPLCLTGHIHEQWGFEEIEGTVYLNPSNFGEVPEPGGRVAEGGFFY
                     DIETKGRQIARVTLSKLVGRTVHQVAVYTRRKGAWTKYVLDPERFGALLRGKNHDWRE
                     TQSKDRHVEIKKNMKWFFPGSATDGELQAVLGEINHFASDLKYRFEVSLSADFLGDVH
                     GPDSPPSPPLDIILYLRCGSSPGPPKFQQKDNGRPICLEVVEMTRNALSPHPYVRVAD
                     WVDLDVVSRSLAERDYECDALLRFAAYRVAGRRILDDGAASVERELDADRAFRGEVEG
                     TGHAYMEIFRNVAERVENMKVFETRLRELGIAAPEAFKIRIRECFYGGRETKDKEDH"
BASE COUNT         2348 a         2834 c         2720 g         1883 t
ORIGIN      
        1 ccggaagagg ccggcaaatt caagcgcgtc ttcttcctcg accccttcat cgccggcgac
       61 cgcctggaac acgcgatgat tttctacgac atcgtgaaaa gaaacagaat ccagtgttat
      121 ctcgccaaca cggggacgat cggccaacag gaggaaaagg tcaccctccg ccaatccctg
      181 gcggcataca acgacctcgt ccgtatgcag ctccgtttca gccgtgagcc cgatgccatg
      241 gggtactact acccgatcaa gtgcgaccgg gccaatcttg acatgatgac ggcctttcaa
      301 ctcttccagg atcgggacat cttcaagaac agggtcgtgg acttcttcaa gggcaggcaa
      361 aagttcctcg aggatttcga gggtaaatac ggcaagatac cggcaaacat cagggagtcg
      421 cttccctaca gctacaagaa cgcctcgccg agggaaatgc cggtcgaatg acccttaatg
      481 ccgtaaaatc aaaacccccg ccgagaaaag gcaaggtcgc taaaatgccg gaaatctcgg
      541 tgttatttct tctatgtccc gcaccgggga accgcatatc aggtttaaga cggcttcacc
      601 gatcgaatcc cgggttacac cgttcacatt ctccccgatc gcccgcgact tgagcagctt
      661 ttcgttcctg tcgtaatcac ttacgctcaa ctggcggatc gttacctcct tgcaatcgag
      721 ctccataagg cccagttcgt aggcccacct gtcgtatcct tcgataggaa gtccgtcttt
      781 ttttctttcc cggacggcaa tttcccgctg cttatcgtcc accatccttt tgagccagac
      841 cagggctttc ctttccggca gatatcggac ggtcgttccg tcgtagaacc actggcaatc
      901 gttggtatct ttcccgaccg gcttccagtc tgcaccgtgc gattccgacc cgggcccgcc
      961 cagaagggaa aaaacgaaaa ggacgataaa gacgctccgc cccaaggttg tccctatcct
     1021 tcccggtaaa ctgtctatca tgccgtcagc atatatcttt cgcccaggaa tctcaatccc
     1081 cttctgccgt tttcactttt ttcgctgatt tttccacgga ccgctaaagt tctgccgcgg
     1141 gatggcgata atatagtgag gcggtgccca accgaagatt gcgtcattta aactcggcgt
     1201 cgaggactat ccatgggaac gatcaacctc gaagatatcc aacctggcat gattcttgag
     1261 gaagcggtag aggaccgcag cggccgcatc ctcctagggg caggagaaga ggtcaccgaa
     1321 aggcacatac ggatattcaa gatgtggggc gtgacggaag ccgccgtcaa gaatgtcggg
     1381 gaagaggatg tgtctgcgaa aaccgccgca tctgtcgacc ccgtgctgct ccgagagacg
     1441 caaaataccc tgcgggagtc gttccgccat accgacatgg gtcacgcctt caacaaggaa
     1501 tttctccggc tcctcagtct ccgcatggcc gcacgggagg gccgaagatg agaacaacgg
     1561 acggccttga ccttgaacgg ctttttagag acgatgtcca ggttccgtct ctgcccatca
     1621 tctacaggag gataaacgac gcagtcaaca acccgcgttg ctccacctcc gatataagtg
     1681 ccataatcgg agacgacccc ggcctcacat cgaggcttct tcggctcgtc aacagcgcct
     1741 tctacggata cccttccggg atcggcacga tcagccgcgc ccttctcatc gtcggcaccc
     1801 atcaactgcg cgacctcgcc ctcggcacct ccatcatcga acttttcaaa ggcatctccc
     1861 gaaccctcgt ggacatggaa tctttctgcc gccacagcat cgcctgcggt cttgcagcca
     1921 agatactggg aacctactgc caaacacagt ccacggccga gagcttcttt gtggccggcc
     1981 tcatccacga catcggccgc ttgctcatct acaaaaagag gcccgaatcg gccggtaaag
     2041 ccctcgccct tgccagggac aagggtgaac tgcttcataa gtccgagcgg gcggtcttcg
     2101 gcttcaacca cacggacgtg gggcgccttc tcatccgccg ttggaacctt ccgccgagcc
     2161 tcgaggaagt cgccgcgtac caccacaacc cgacaagcgc cgtcttctac ccgatggaaa
     2221 gcgccgtcgt ccacgtggca gacatcatcg ccaacggtat gcagttaggc agcagcggcg
     2281 agtgcctggt gcctccgctc aatgaacggg catgggaatt gctcggcctt cgggtcagcg
     2341 tcctttcgcc ggtcatggac cagctcgaaa gggaagctgc ggaggtccaa caactgatat
     2401 tcgagaacac ttgaccgtga acgaccggct tttaaaactt gaacaacggg tccaatacct
     2461 ggaagagctc aacaggtcaa ccttgagggc cttggacctc gccatgtctt tctgcgattg
     2521 ccagaacagg ctcagcctcc cccacctcga caaccgcatc atcttcaact cggcctgttc
     2581 atacctgaag cggatcatcc ccttcagggc gatggcgttc tacctgaccg acgaagagga
     2641 ccccgaattc aggctcaaga gctgcaaccc tttcgccgag cggcctacga tagaagagga
     2701 cgtggaccgc caggtctcga acggcgtctt tgcctgggca ctccgccaaa accgggcggt
     2761 gatcattccg tcctctaagc cagaccgaaa ggtcgtgctc cattccctgg ttaccgcctc
     2821 gcagacgatc ggcatgttcg tcggcatcct ggacgacggg gagatcaagg tcgacaagaa
     2881 catcgcatac ctcctaacca tcatcttttt caatacggcc cggtctctcg aaaacgccgg
     2941 tctcttcaag aaggtcagag accataacct ggacctggag cggacagtta aaagaagaac
     3001 ggccgagctt aatgccgccc tcgagaatgc tgaggcggcg aacgttgcaa aaagtcgttt
     3061 cctcgccaac atcagccatg agatccggac gccgatgagc gcagtgatcg ggttcacaga
     3121 gctgctcgcg aaaaccgagt tgaatcgcga gcaatctgat tacgcaaaca tgatcaaact
     3181 aaacagcgag attcttctcg acctgatcaa cgacatactc gatttttcca aggtcgaagc
     3241 ccggaggctg gaactcgagt gcgccgactt caacccggaa ctcacggctt acgaggtttg
     3301 cgaagtgctg ctgaccaggc ttgcccataa accggttacg ttgtcctgcc gcatcgggga
     3361 cggcctgccc ctattcgtct gcggcgaccg tcatcgattc cgccaggttc tgcttaacct
     3421 cgcccacaac gccgtaaaat tcaccgactc cggcgagatc gtcgtttccc tcgacaaaga
     3481 ggacgagagg gacggtgccg tcaagataca cgtctccgtc agtgacactg gtgtcggtgt
     3541 gcccgaagag aagttgcagg tgatcttcga accattccgc caggccgacg gctcgtcgac
     3601 gaggagatac ggggggaccg gcttaggtct cgccatttgc aaacagcttg ccgaactgat
     3661 ggaaggcgac acgtgggcgg aaagctctcc gggaaagggg agtacgtttc acttcactgc
     3721 atggatgaaa agatcgaagc aggacagggt gagagacttt taccacccgg cgctcgcggg
     3781 caaacgggtg ctcctcatcg acgagagcaa atcccacctg agttcgctcg cgcacttact
     3841 cgagtcggcg gggatgcggc cgactacggc aacggaagtc ggctttatcc ttgagtccct
     3901 caaaggagct attgaagaaa aagaccctta cgaattcatc atcgtcaatg taaaaacgcc
     3961 cggcttaagg accgacgggt tcgctgagaa agtccgcgca gccggcgggc cgttgtacct
     4021 ggtcgccgtc gggtccgctg tcagaccctc ggcagaaaaa tacaagaagg ccgggttcaa
     4081 cctcttcgtc cggatgccgg cccgtaggga caagttcgtc cgcgacctcg ctaacctcgt
     4141 cgaaagccac gatgaggagg gcaggaaagg cgaagacaaa accgtccggc catgcatgag
     4201 aaaggggccg cggatactgc tcgcggaaga caacccagcc ggcaggaaac tcatgaacat
     4261 cattctgaac aaagagggct gcagggtcga gacggccacg aacgggcgcg aagccgtcga
     4321 taagtttctc catgccaggg gtgctttcga cctaatcttc atggacatac agatgcccgt
     4381 cgtcgacggt gtagcggcgg ccagggcgat ccgggaaagg ggattcaggg agatcccgat
     4441 catcgcactg acggccagtg cggtcgacga agaccggcgg tcctgtgaag aagcagggat
     4501 gaacgattat ctcataaaac cggtggcaag agagcggttg atcgaagtta ttgatagatg
     4561 gacaaacaga ggacgcaacc atggacctca ggagattggc cgagaggctc gggctgggcg
     4621 aggctgaatt caaggaagtc gtgaggctct tcatcgaatc cagcatcgcg gacatggagt
     4681 cttttacttc cgcagtaaga accggcgacc cggcaggggc gtcggcggcg atccattcca
     4741 taaagggcgc ggcgctttgc ctcgggctga acgacatatc ccgggctgcc cgggagatcg
     4801 aactccgtag ccgtcaaggc cgcctcgacg gattggccga ggcaaccgct gcgataaaga
     4861 gggagatcga gaaaatagcc ggctacgccg gcgaaagatg agtagacggg ggataaggac
     4921 atgcataatg cgatcaaaga cagaaagtta cacgagacga ggccggtcaa caaggtgcta
     4981 gttgccgaca acaaccctgt catgctgacc ttgttgcaca atatcctcgc gaagaacggc
     5041 tgcgaggtgg tcacagccga agacggcctg accgtcctcg aaatactcgc gagttacaag
     5101 cccgatgtca tattcatcga cctgatcatg ccgaacatcg acggggagag gctctgccgg
     5161 atcatccgta gcatgcctca tatgaacgac gtcgttttgg taatcatcag cgctgtcgct
     5221 gccgaaacga agataccttt taccgaatgg ggcgcgaacg cctgcatcgc caagggacct
     5281 ttcgacaaga tgtcccgtag catcaaggat ctcctcgaaa ggatagaaac cgacaccttc
     5341 ttccctgccg ggtggagggt gctcggccct gattatgccc gcccgagaga gataacggaa
     5401 gagctccttt ttctgaagag ccatcttgag acgctcttgt cgagcatctc ggagggcata
     5461 atcgaaataa cgcccgaggg cagggtcgta tacgtcaatc ccgaggcggt tgaaatcacc
     5521 ggcggttcgg aagagaaaat ccttgccacc gacatcacgg cgctgttcaa agaggaggaa
     5581 tacccaaagg tccgcaaggc cctcatgagc gcgagggcga actaccgcac cgccgaggaa
     5641 tccgtccttg cgaacggcaa agaggtctca ttcaccgtcg tcccggttaa aaacgaccgg
     5701 aacaaactcg tgatcctcag tgacctgggc ctgcgcaaac acctggaggc cgagttgcgg
     5761 caggcgcaga aactcgaggc catcggaacc cttgctggcg gtatcgccca tgatttcaac
     5821 aacctactga tggagatcca gggccgcacg tcgctcatgc tcttgaaggc aggcgaaaac
     5881 gacttcctga agcaaaacct caaggtgata gaagacatcg tcatgagcgg cgcgaacctc
     5941 acccgtcagt tgctcgaatt cgcccagaac ggcaagtatg tcgtcgaacc cacgaacctg
     6001 aacgacctcg tgcagagaag cgtcaagatg ttcggccgca ccaagaaaga gatagtcctc
     6061 gacttagacc ttgaggaaaa tctgtgggcg gtggaagtcg accgcggtca gatcgaacag
     6121 gcccttttaa atctttacat aaacgcctgg caggccatgc cgggcggcgg caccatctac
     6181 ctcaagacag aaaacgttcg gatgaacgac aaagaggccg acattcgtcg catgaaaccc
     6241 ggccgttatg tcaagctgac cgtcagggac accggcacgg ggatggacga gcagacaagg
     6301 cagaggatat tcgaaccatt cttcacgacg aaagaaaagg gcctaggcac agggatgggc
     6361 ctggcctccg tctacgggat cgtcaaaggc cataacggct ccatagaagt caagagcgag
     6421 cggggagagg ggactacgtt caccatctac ctgcctgcca cggggaaaaa ggtcccccac
     6481 gaaagaaagg ccgaggagaa gatactgccg gggaaggaaa ccatcctgtt ggtcgacgac
     6541 gaagaggtcg tgacgaatgt agtccgcgag atgctcgaga gcctgggcta cacggtcatc
     6601 gcggccctca acgggaagga cgccctcgag ctgtaccgcc aaaacaaaga tcggatagac
     6661 ctggtcatcc tcgacatgat catgccgcag atgagcggtt acgaggtatt cgaagccctc
     6721 aaggcactca acccaaaagt aagggtgatc ctcgcaacgg gttacagcat gaaagagcag
     6781 gcggccaaga tcatggaaag cggctgccgg gccttcatgc agaaaccgtt cagcatccag
     6841 gatctgtcga acaaaatcag gaccgtcctc gacgcggccg aatgactttg ccttttcagg
     6901 tttctatcaa tgtggccgtc cttgacagtg tggagtagcg aatgataaat tccttgctaa
     6961 actttccttt gttttacaaa ttcgaccctg attcgggtag gagattgcgg agacgtgttg
     7021 gaaaacgccc aaaagaggaa gaaaagggcg gccgccctct cggtggcttc caatacggtc
     7081 ctcatcgtga tgaaaatcgt cgttggaata gcgatcggct cggtttccgt catctccgag
     7141 gccattcatt ccagcgttga cctcctcgcg tccgccatcg cgctgttttc cgtttcgacg
     7201 tcggacaagc ccgccgacaa agagcaccct ttcggacacg gtaagatcga gaatatctcg
     7261 gggaccgtcg aagcgatcct gatcttcttg gccgcagcct ggatcgttta cgaagccgcc
     7321 cagaagctca tacaccccag gccgatcgac actgccctcc tcggagtcgg ggtaatgttc
     7381 gtatccgccg tcacaaatgc cgtcttgtcg cactacctgc tcaccgtcgg gagggagacc
     7441 gactccatcg ccctccaggc cgacgcctgg cacctgagga ccgacgtgta cacctcggtg
     7501 ggggtcatgc tcagcctcgc cttcatatgg ctcggggaac gggtgtcgcc cggcctcgac
     7561 ctcaactggc tagacccggt atgtgccgtc gcggtggccc tcttgatcac gagagccgct
     7621 taccaactga cgatacagtc cgcccgcgac cttctcgacg cacatctgcc gccgaaagaa
     7681 gagaactgga tcaggaacct gatcgaggcc aatcggggtg tcatccacgg ttaccattat
     7741 ctgcgcaccc gcaaagcagg gcgttttcgc ttcgtcgagt tccatatcca ggtcgacccg
     7801 aagatgaccg tagagctttc ccacagactg gcgcaaagat tgacccggga aataaaaaac
     7861 cgcttgcccg acactaccgt aaccgttcac atcgaacctt gtctgggcga ctgcagcgaa
     7921 aagtgtcttt ccggatgtct gctcacggag tcggagcgac ttagggcatt cggtcatcgg
     7981 ggcaagattg tgcaaccggc gcaagtactg aagtaacgcg aaaaatctga ggcaatcgtc
     8041 ccatgaagat catctatctt gccgatgtcc atggagactt cccgcgggtc aaggacctcc
     8101 tggccgtcac ggacgcccaa gtgtacatcg tcgccggcga ccttatcgac cgccccttct
     8161 tcactgagga gatggccgcc cggtaccgtg agttgcaatc gtacttcttc cgcctccagt
     8221 gcaggatggg agacggagga acagacatgg acgattttgt agaggacctc ctgagacaga
     8281 cggacctgcc gaacgacatc ctggcgaacg ccagggaata tcaagagaaa acggtgaggg
     8341 cgcgccgcgt cctccagcag aagtataaag tgctggagac gatcctttct ctcaagagaa
     8401 attcgctgat cttttgcctg ccgggcaact acgacatgga cttgcagtac acttctctcc
     8461 acagccgcga tcttcaccgt cattggtatt atgtcgcaga cctgcgcatc gccggctacg
     8521 ggggggcgga cgtggcgacc cctggtatcc cccatcgcta cgccgttccg tacaacgcgg
     8581 acaggggaat gagcgagatg taccgttttt tcaaagagac ccgcccggac atcgtcgtga
     8641 cccacaaacc cgcccacggt gtacacgacc gcgtcttgcc catgggtgag agcggttcgc
     8701 tggaactgag gagattctgc gaagaaaatc acgtacccct ttgccttacc ggtcacatcc
     8761 atgaacaatg gggctttgaa gaaatcgagg gaaccgtcta cctcaaccct tccaacttcg
     8821 gcgaggtgcc ggagcccggg ggccgggtgg ccgaaggcgg ttttttttac gacatcgaaa
     8881 cgaagggcag gcagatcgca agggtcaccc tcagcaaact cgtcgggagg acagtccatc
     8941 aagtcgcggt ttacaccagg agaaaaggag cctggacgaa atacgtcctc gacccggaac
     9001 gtttcggcgc actcctgaga ggaaagaatc acgactggag agagacgcag tccaaggacc
     9061 ggcacgttga gataaagaag aatatgaagt ggttctttcc cggttcggca acggatggtg
     9121 agcttcaggc cgtcttaggg gagataaatc acttcgcttc agacctcaag tatcgctttg
     9181 aagtgagtct atcggccgac tttctcggag atgtccatgg accagactcg ccgccatcac
     9241 cccccctgga catcatcctc taccttcgct gcggttcgag tcccggaccg cccaagtttc
     9301 aacagaaaga caacggccgt ccgatatgtc tggaggtggt agaaatgaca agaaacgccc
     9361 tttcacccca tccgtacgtc cgggttgccg actgggtcga cctggacgtg gtgagccgca
     9421 gcttggcgga aagagactac gaatgcgatg cccttctacg ctttgccgcg taccgggttg
     9481 caggcaggcg tatcctggat gacggcgctg cctccgtaga gagggagctc gacgccgacc
     9541 gggcattccg gggtgaagtc gagggaacag gtcatgctta catggaaatc ttccggaatg
     9601 tcgccgaacg ggtggaaaac atgaaggtct tcgagacccg gctgcgggaa ctcggtattg
     9661 cggcaccgga ggcgttcaag ataagaatac gggagtgttt ctatggcggt cgcgaaacaa
     9721 aagataaaga agatcattaa gatgggagac ttgctgtgcg cctcctacac acttgaggag
     9781 gccta
//
LOCUS       DAOTMX010000020         9559 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_6588_length_9559_cov_3.540194, whole genome shotgun sequence.
ACCESSION   DAOTMX010000020 DAOTMX010000000
VERSION     DAOTMX010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 9559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9559
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6588_length_9559_cov_3.540194"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="P5573_01130"
     CDS             <1..197
                     /locus_tag="P5573_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT26702.1"
                     /translation="GDYNKIRQGDRLEIDNLRQALEKREEVRVKNLTGGSEFKTFFEL
                     TDRQVALVLAGGALNYGKGR"
     gene            249..1463
                     /locus_tag="P5573_01135"
     CDS             249..1463
                     /locus_tag="P5573_01135"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HRT26703.1"
                     /translation="MTKIWNKTPIVEMDGDEMTRVMWRMVKDKLLLPWVDLNTDYYDL
                     HIKHRDETDDQVTIDAARAIMKYGVGVKCATITPNQDRVIEYGLKKQWKSPNGTIRAM
                     LDGTVFRKPIMVRNIKPLVSTWKKPITIGRHAAGDVYEGSEMLVREGGKAELVFTSSS
                     GQVSRQTVKDFRGPGIVQGIHNTDDSIRSFARACFAYALDEKVDLWFSTKDTISKVYD
                     ARFRDIFQEEYETNWRQKFADSRISYFFTLIDDAVARVVRSEGGMLWALKNYDGDVMS
                     DMVASACGSLAMMTSVLVSPKGWYEYEAAHGTVQKHYYKHLAGEETSTNPMALIFAWS
                     GGLRKRGEIDGTPDVVRFADALEAAAVETVESGVMTGDLLAVAEKSQADRKVNTEDFI
                     DAIAENLRRKLS"
     gene            1426..2517
                     /gene="rlmN"
                     /locus_tag="P5573_01140"
     CDS             1426..2517
                     /gene="rlmN"
                     /locus_tag="P5573_01140"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HRT26704.1"
                     /translation="MPLPRTFEESCHNDSAVRDLPDVKDMTPEELERFVAFTGKEGYR
                     SRQIMKWVYRFGVRSFEEMTDLSKAFRQELKGLARISELEIRKVQDSKDGTKKVLFRL
                     EDGLHVESVLIPGKRHRTLCVSTQAGCRMGCRFCLTGRGGFKRNLRVSEITDQITVCR
                     FRLPEGKEIDNIVMMGMGEPLDNYENVVRAVRVITSDWGLAISNRKITVSTCGIVPMI
                     ERLSNDAQVNLAVSLNATDDETRASLMPVGRLYPLKALLSACRKYQMPRRRRITFEYI
                     LIDGVNSSLADAEKLARLLRGLRCKVNLIAFNEYPESEFRPPPAEAVEAFRSHLIKKG
                     FTAILRASKGSDILAACGQLSALERGDGR"
     gene            2514..4091
                     /locus_tag="P5573_01145"
     CDS             2514..4091
                     /locus_tag="P5573_01145"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRT26705.1"
                     /translation="MTHPGLVEAMADPRFYPHRPDRVELVQTHISYIFIAGDLVFKVK
                     KAVDFGFLDFTTLEKRRYYCREELRLNRRLAPEAYLEVVEIREDDGRLFLGMDRGEVV
                     EYAVKMVRLPRERMLKKLLAEGRVTSSVMDAVANKIADFHAAADTGGKIDEIGGVDTV
                     RRNNEENFEQTRKYIGRTIRKDQYDFVEVYVFRFMEEQNELLHKRVRDHRIRDCHGDL
                     HLEHICLTDGIVVFDCIEFNERFRYEDVAAEVAFLAMDLDFNGYPDFGDRFVESYCRY
                     ARDPEVKILLNFYKCYYAYVRGKVTSFRSDDEVVTIDERKEAGETASRYFDLAYRYAA
                     RPEKPVVVVMTGLMGTGKSVLAGNLAPLLGAEVIRTDVLRKDLLEIKPTERHYEAFGE
                     GIYSEELTEKTYERAIEDARRKLREGRSVIIDASFRRRHDRMKVRAMSREEGADFFVI
                     ECVCPEEVIKKRLDARVKEREEPSDGRWELFAEQKKRFEPVEEYTAKEHIVIDTSGPV
                     ELTVGRAIRRIRFGSDN"
     gene            complement(4088..4819)
                     /locus_tag="P5573_01150"
     CDS             complement(4088..4819)
                     /locus_tag="P5573_01150"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HRT26706.1"
                     /translation="MSILVLIAIYGCGFFTRSQMPLNSPEALYDRAVWYYHKEDYKKA
                     IELFQRVKEEYPLSKHALMAEIGIADSYFSAGLFVEAEMNYTEFINLHPTNPNVPYAI
                     YQLGMCHYKQMSTIDRDQTETLKAKKEFERLVSQFPASRFAALAQQKLRECKKRLAEH
                     EFYVGHFYFKMKQYKAALKRFELIEKEYPNLGLDYKVAYFLAETRRRIEVEERERLAR
                     EREEKEKETLKNKKKTDSGKDKKKG"
     gene            complement(4863..5195)
                     /gene="trxA"
                     /locus_tag="P5573_01155"
     CDS             complement(4863..5195)
                     /gene="trxA"
                     /locus_tag="P5573_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRT26707.1"
                     /translation="MAEESPVIHISDDDFETNILKGDKPVLVDFWAPWCGPCRAIAPT
                     LEELASQFKGRVKIAKINIDDHPKTAASYNVRSIPTLILFKDGKAVETVVGLTSKDRL
                     ENLLKKVL"
     assembly_gap    5312..5411
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5412..6208
                     /locus_tag="P5573_01160"
     CDS             <5412..6208
                     /locus_tag="P5573_01160"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MTAP family purine nucleoside phosphorylase"
                     /protein_id="HRT26708.1"
                     /translation="SKSRRSSMGFDSDFFICYKRVNRQAGEGMERLGIITGTLTLREK
                     VFLETTEKKSVENEYGKALVYLGRDVVLVPRHGEAGKYVLPHLINHPANMKALKDLGV
                     EEVIAVNSTGSLKKNLAPGMIVLPDDFIFLAGGPTVFREEAVHITPDLSPKVRLRLVE
                     AAADAGVTVIDKGVYWQTIGPRLETKAEIRMMAGYADVVGMTMASEAVVAKELGMEYA
                     SLCSVDNYAHGVEERELSMEQILRQSRENAESVSRVVSKYVERRGG"
     gene            6205..6732
                     /locus_tag="P5573_01165"
     CDS             6205..6732
                     /locus_tag="P5573_01165"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HRT26709.1"
                     /translation="MKFSKKGDGGETSLLGGQRVPKYDPRPDTYGTLDEASSALGVAR
                     AMTKDVRIKEIILEVQKDLLVLGAELAALPEDCKKLGKLVGLEHIRRLEDTIDELQKS
                     VKLKNEFIYPGESLAAAQIDLGRTIVRRAERKAAKLKNDGLIDRPEINQYLNRLADML
                     FTLARYEEETSRVKD"
     gene            complement(6729..7157)
                     /locus_tag="P5573_01170"
     CDS             complement(6729..7157)
                     /locus_tag="P5573_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT26710.1"
                     /translation="MKKDKKLSKKHPPDRKIDQMLAGEIRSTAKDGELTCAQALGIAA
                     AIGVNPEEVGFTMDALGIRISMCQLGLFGYRPVSRILKPAETVDHSLAEAIQCALVDG
                     RLSCAASWEIAKNFRMAKIKVASACEALKLKVSSCQLGAF"
     gene            complement(7186..7641)
                     /locus_tag="P5573_01175"
     CDS             complement(7186..7641)
                     /locus_tag="P5573_01175"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HRT26711.1"
                     /translation="MITRQISVFAENKPGSLARVTGILAREKINIRATTIATSDTFGV
                     INLIVDDPDRAFKALTREGVMATMKDVIAVVIDDKPGGLDKLTQCLFKENININNAYG
                     FVLESWKTAVFVLDVDQKEKTVEVLKQKGFKMLDTDALSSLEPFHYIKY"
     gene            complement(7658..8956)
                     /locus_tag="P5573_01180"
     CDS             complement(7658..8956)
                     /locus_tag="P5573_01180"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HRT26712.1"
                     /translation="MYWEKEIEAMPRRELEALQLSRLKETLNRAKASTHYREKFNEIK
                     FDPDSLGSLEDLGSIPLTTKDDLREHWPYGFLSVPKDDLVRMHSSSGTTGRATVVFHT
                     LKDIQGWADLIARCMYMAGVRRSDVFQNMMTYGLFTGGLGFHYGAEKVGALVIPAGTG
                     NSKRQIQLMRDFDTTTIHIIPSYALHLYTTFEEMKIDPKKDTRLKFAFLGAEPHSEKT
                     RLKIEELYGLKAFNSYGLSEMNGPGVAFECPFQNGLHLWEDHFLMEIIDPDTLKPLPE
                     GEEGELVLTTLTREGMPILRYRTKDLTRVMPGPCPCGRTHRRIERIKGRTDDMLIIKG
                     VNIFPIQVEKKLMEIPGVGRNFQIILEREGFNDDMIVRVEVEKEYFDGDILHLERLQR
                     RIVEELKSDILITPKVELVEPNTLPKGEGKAQRVIDNRKE"
     gene            9134..>9559
                     /locus_tag="P5573_01185"
     CDS             9134..>9559
                     /locus_tag="P5573_01185"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HRT26713.1"
                     /translation="MTGIVLCGGKSSRMGTNKAFLEVEGERLIDRVVWVLGRHFAEVV
                     LVTRTPTDYQSIDAVIVKDMIPEGGVLGGIYTGLFFSSAEIAFVAACDMPFLDDRFID
                     HMIRRAENYDIVVPMTREGFQPLHAIYSRRCLPEMLRMIE"
BASE COUNT         2144 a         2395 c         2829 g         2091 t
ORIGIN      
        1 aaggcgacta caacaaaatc cggcagggag accgtttaga gatcgacaac ttgaggcagg
       61 ccctcgagaa gagggaagag gtgagggtga agaatctgac cggggggtct gaattcaaga
      121 cgtttttcga actgacggac cggcaggtgg cgcttgtgct cgcgggcggg gccttgaact
      181 atggaaaagg ccgatgatca atccggtaca gggccgatat tgagaagaac aggaagatta
      241 ggaagactat gactaaaatc tggaacaaga cgccgatagt ggagatggac ggcgacgaga
      301 tgacgcgtgt gatgtggcgg atggtgaagg acaaactgct tttgccgtgg gtcgacctga
      361 atacggacta ttacgacctc cacatcaaac accgtgacga gacagacgat caggtgacga
      421 tagacgccgc ccgggcgatc atgaaatacg gggtaggggt caagtgcgca acgatcacgc
      481 cgaaccagga ccgggtcatc gagtacgggt tgaagaaaca gtggaaaagc ccgaacggga
      541 cgatcagggc aatgctagat gggacggttt tccgcaagcc catcatggtg aggaacatca
      601 aaccgctcgt ttccacgtgg aagaaaccca taaccatcgg acggcatgcc gccggtgatg
      661 tttatgaagg ttcggagatg ttggtccggg aagggggaaa ggcggagctc gtcttcacgt
      721 cttcttcagg gcaggtctcg cggcagacgg tcaaagactt caggggcccg gggatcgtcc
      781 agggaataca caacaccgac gattcgatca ggagttttgc ccgggcatgt tttgcctacg
      841 cactggacga gaaggttgac ctgtggttca gcaccaagga tacgatatcc aaggtctacg
      901 acgcccgctt tcgggacatc tttcaagaag agtatgagac gaactggaga cagaaattcg
      961 ccgattcccg catttcctat ttctttaccc tgatcgacga tgccgtggcg agggtcgtgc
     1021 gttcggaggg tgggatgctc tgggcattga agaattacga cggggacgtc atgtccgata
     1081 tggtggcctc ggcctgcgga agcctggcga tgatgacgtc ggtcctcgtg tctccaaagg
     1141 gatggtacga gtacgaggcc gcccacggga cggtgcagaa gcactactac aaacacctcg
     1201 ccggcgagga gacgtcgacg aaccccatgg cgctcatctt cgcctggtcc ggcgggctca
     1261 ggaaaagggg agagatagac gggacgccgg atgtcgtgag gtttgccgac gccctcgagg
     1321 cggcggccgt cgagaccgtc gaatcggggg tgatgaccgg tgacctcctt gcggttgccg
     1381 aaaaaagtca ggccgacaga aaagtaaaca ccgaggattt tatcgatgcc attgccgaga
     1441 accttcgaag aaagctgtca taacgactcg gccgttcgcg accttccgga tgtcaaggac
     1501 atgaccccgg aagagctcga aaggttcgtt gccttcaccg ggaaggaagg ctaccggtcc
     1561 aggcagatca tgaagtgggt ctatcggttc ggcgtcaggt ccttcgaaga gatgaccgac
     1621 ctttccaagg ccttccgcca agagttgaag ggcctggcca ggataagcga actcgagatc
     1681 agaaaagtcc aggattcaaa agacgggacg aagaaggtcc tcttccgcct cgaggacggc
     1741 cttcatgtcg agagcgtcct catcccgggg aaacggcacc gcacgctctg cgtctcgacg
     1801 caggccggtt gccggatggg gtgtcgattc tgtctcaccg gtcggggcgg tttcaagaga
     1861 aatttaaggg tgtcggagat cacggaccag ataacggtgt gtcggttcag gttgcccgaa
     1921 ggtaaagaga tcgacaacat cgtcatgatg ggcatgggag agcccctcga caactatgag
     1981 aacgtcgtga gggcggtgag ggtcatcacc tccgattggg ggctcgcgat ctcgaaccgg
     2041 aagataaccg tctcgacctg cggcattgtc ccgatgatcg aaaggctgag caacgacgca
     2101 caggtgaacc tcgccgtctc tctgaatgcg acagacgatg agacgagagc aagtctcatg
     2161 cctgtcggca gactctaccc gctgaaggcc ttactcagtg cctgcaggaa gtatcagatg
     2221 ccgcgcagga ggaggatcac cttcgagtac atcctgatag acggcgtcaa ctcatccctt
     2281 gcagacgcgg aaaagctggc ccggttgctt cgagggctcc gctgcaaagt aaaccttatc
     2341 gctttcaacg agtatccgga gtcggaattc cggccaccgc ccgcggaagc ggtcgaagcc
     2401 ttcagaagcc acctgataaa gaagggattc actgcgatcc tacgggcgag caagggatcc
     2461 gacatcctgg cggcatgcgg ccagttgagc gccttggaaa ggggtgacgg cagatgaccc
     2521 atcccgggct cgtcgaggcg atggcggacc cccggtttta cccccacagg ccggaccgcg
     2581 ttgaactcgt ccagacgcat atatcgtaca tcttcatcgc cggagacctc gtcttcaagg
     2641 tcaaaaaggc cgtggacttc gggttcctcg acttcacaac cctcgaaaag aggcgatact
     2701 actgccggga agagttgcgg ctgaacaggc gtctcgcccc ggaggcgtat ctcgaggtgg
     2761 tggaaatacg agaggacgac ggccgtctat ttctcggtat ggacagaggg gaggttgtgg
     2821 aatacgccgt caaaatggtg cgactgcccc gggagaggat gctgaagaag ctgctcgcgg
     2881 aggggcgggt gacttcttct gtgatggacg ctgtagcgaa taagatagct gactttcacg
     2941 cggcggcgga tacgggcggg aagatagacg aaataggcgg ggtcgacacg gtccggcgga
     3001 acaatgagga gaacttcgaa cagacccgca agtacattgg ccgtacgatc aggaaagacc
     3061 aatatgattt cgtagaggtc tatgtcttcc ggttcatgga agagcagaac gaactcctgc
     3121 ataagagagt cagagaccat cggatacggg actgccacgg agacctccac ctggagcaca
     3181 tctgcctgac ggacgggatc gtcgtcttcg actgcatcga attcaacgag cgtttccggt
     3241 acgaggatgt ggccgctgag gtcgcgttcc ttgccatgga cctggacttt aacggctacc
     3301 ccgatttcgg cgatcggttc gtggagtcct actgtcgata cgcccgcgac cccgaagtca
     3361 agatactcct caatttctac aaatgttact atgcctacgt caggggtaag gtgacgagct
     3421 tcaggagcga cgatgaggtg gtcactatcg atgagaggaa agaggccggg gaaacggcgt
     3481 cgcgatactt cgacctcgcc taccgttacg cggcgcggcc ggaaaaaccg gttgtcgtgg
     3541 tgatgacggg tctcatgggg acggggaaga gcgtgctcgc cggaaacctc gcacccctcc
     3601 tgggagccga ggtcatcagg accgatgtcc tgagaaagga cctgctggag atcaaaccga
     3661 cggaacgtca ctacgaggcc ttcggcgagg ggatctattc ggaagaacta accgaaaaga
     3721 cctacgagag ggcgatcgaa gacgcgaggc gcaagctgag ggaaggcagg tcggtcatca
     3781 tcgatgcatc attcaggcga cgacacgacc ggatgaaggt cagggccatg agccgcgaag
     3841 aaggggcgga tttttttgtc attgagtgcg tatgccccga agaggtcatc aagaaacggc
     3901 tggatgcaag ggtgaaggag agggaagaac cgtccgacgg ccggtgggag ctcttcgccg
     3961 aacagaaaaa acgatttgag cccgtggagg aatacaccgc taaagagcat atagtcatcg
     4021 atacgtccgg gcctgtggag ttgacggtgg gcagggctat tcggagaatc aggttcggca
     4081 gcgacaacta acctttcttt ttgtcttttc cactgtctgt cttcttctta tttttcagcg
     4141 tctctttttc cttttcttcc cgctctctcg ccagtcgttc ccgttcctcg acctcgatcc
     4201 gcctccgcgt ctccgccaga aaataagcta ccttgtaatc aagtccgagg ttgggatatt
     4261 ccttttctat tagctcgaac ctcttcagcg ccgccttgta ctgcttcatc ttgaaataga
     4321 agtggccgac gtagaattca tgctcggcca gtcgcttttt acattcgcgc agtttctgct
     4381 gtgcgagagc ggcgaatctg ctcgcgggga attgggagac gagcctttcg aactcttttt
     4441 ttgccttcag cgtctccgtc tggtctcggt ctatagtcga catctgcttg taatggcaca
     4501 tacctaactg gtagatggcg taaggtacgt tgggattggt cggatggagg ttgatgaact
     4561 cggtgtaatt catctccgcc tcgacaaaaa ggccggcgct gaagtaggag tccgctattc
     4621 cgatttcggc cataagggcg tgcttgctta acggatattc ctctttgacg cgctggaaga
     4681 gctcgattgc ctttttgtaa tcctccttgt ggtaatacca gacggccctg tcgtacaggg
     4741 cctcggggct gttgaggggc atttgcgaac gggtaaagaa gccgcagccg tatatcgcaa
     4801 ttaggactag gatcgacaag aaaaaagcag tcctgaacct tctcgagctt gcttgtctca
     4861 aatcagagca ccttttttag gagattttcg aggcggtcct tggaagtcaa accgacgacc
     4921 gtttcgacgg ccttgccgtc tttgaaaagg atgagagtgg gtatactcct gacattgtac
     4981 gaagcggctg tcttgggatg atcgtcgatg ttgattttcg cgatctttac ccggcctttg
     5041 aattgcgagg ccagttcttc gagcgtgggt gcgatggccc gacaggggcc gcaccaaggc
     5101 gcccagaagt ccacgagaac gggtttgtca cctttaagaa tgttagtctc gaaatcgtcg
     5161 tcactgatat ggatcacagg actctcttct gccatgaaag gcctccccga atattttcga
     5221 aaaatatgcc acaggatgac gggcgctgtc aatccgaatc gaaaagccgc cggtcatcta
     5281 tgggttttga cagtgatttt tttatctgct annnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn naatcgaaaa gccgccggtc atctatgggt tttgacagtg atttttttat
     5461 ctgctataag cgcgtcaatc ggcaggcggg agagggcatg gagcggttgg ggatcatcac
     5521 aggaacgctg actttaagag agaaagtgtt cctcgaaacc actgagaaga aatcggtgga
     5581 gaatgaatac ggcaaggccc tcgtttatct tggtagggac gtggtgctgg ttccgagaca
     5641 cggggaagcg ggaaaatacg tactacctca tctgatcaac catcctgcga acatgaaggc
     5701 actgaaagac ctcggcgtcg aagaggtgat cgccgtaaat tcgaccgggt cgctgaagaa
     5761 aaatcttgct ccggggatga tcgtcctgcc ggacgatttc atcttccttg cgggaggacc
     5821 gaccgttttc agggaagagg cggttcacat cacacccgac ctcagcccga aggtgcgcct
     5881 cagattggtg gaggcggcgg ccgatgcggg ggtcaccgtt atcgacaagg gggtttactg
     5941 gcagacgata gggccgagat tagagacgaa ggcggagatc aggatgatgg ccggctatgc
     6001 tgatgtcgtc ggtatgacga tggcaagcga ggcagtcgtg gcaaaggagc ttggcatgga
     6061 gtatgcgtcg ctttgttccg tagataatta tgcccacggt gtcgaagaga gggaattgag
     6121 catggagcag atacttcggc agtcccgcga gaatgcggaa tccgtgagca gggtggtttc
     6181 taagtacgta gagaggagag ggggatgaag ttttcgaaaa aaggagacgg gggggagacg
     6241 agcctgttgg gaggacaacg ggttcccaag tacgacccga ggccggatac ttacggaact
     6301 ctcgacgaag ccagctccgc cctcggcgtg gcgagggcaa tgacgaagga cgtcaggatc
     6361 aaggagatca tcctggaggt acagaaagac cttcttgttc tcggggccga gcttgcagcc
     6421 ttgcccgagg actgcaaaaa actcggaaaa ttggtcggcc ttgagcacat cagacgtctg
     6481 gaagacacca tcgatgaact tcagaaaagc gtcaaactca agaacgagtt catctatcca
     6541 ggggaaagcc tcgcggcggc tcagattgac ctaggccgca ccatcgtccg cagagctgaa
     6601 aggaaggcgg cgaagctgaa aaacgacggc ctcatcgacc ggccggagat caatcaatac
     6661 ctgaataggc tggcggacat gctgtttacg ctcgccaggt atgaagaaga gacctcccgc
     6721 gtgaaggact aaaaggcgcc aagctggcag gacgagactt tcagcttgag ggcttcgcag
     6781 gccgaggcga ccttgatctt cgccatgcgg aaattcttgg cgatttccca ggacgcggca
     6841 caggagaggc gaccgtcaac cagggcgcat tggatcgcct ccgcgaggga gtgatcgact
     6901 gtttcggccg gcttcagaat cctgctcacg ggacgataac cgaaaaggcc gagctggcac
     6961 atagaaatcc tgatgccgag ggcgtccatg gtgaagccca cttcttccgg atttacgcct
     7021 atcgccgcgg cgataccgag ggcctgagca caggtaagct cgccgtcctt tgccgtcgac
     7081 cttatctccc cggcgagcat ctggtctatc ttccggtccg gcggatgttt cttcgaaagt
     7141 tttttgtcct ttttcatcat acctctttga gctgaacccc ttatctcagt attttatgta
     7201 atgaaaaggc tcgagtgacg agagcgcgtc cgtgtcgagc atcttaaagc ccttctgctt
     7261 caagacctca accgtctttt ctttctggtc gacgtcgagg acgaaaacgg cggtcttcca
     7321 actttcaagg acgaacccat aggcgttgtt gatgttgatg ttttctttga agaggcactg
     7381 cgtcaacttg tccaggccgc ccggtttatc gtcgatgacg acggcgataa cgtccttcat
     7441 cgtggccatg accccctctc ttgtcagcgc cttgaacgct cggtcagggt catcgacgat
     7501 caggttgatg acgccgaagg tgtcggacgt cgcgatcgtg gttgcccgga tgtttatctt
     7561 ttctctcgca aggatgcccg tgacccgggc aagactgccc ggtttgtttt ccgcgaacac
     7621 cgaaatctgg cgggttatca ttttgttcga tgcctcctca ttccttcctg ttgtcgatga
     7681 ctctttgggc cttcccctcg cccttcggga gggtgttcgg ttccacgagt tcgaccttag
     7741 gtgttatcag gatgtcgctc ttcaattctt cgactattct gcgctggagg cgctcaaggt
     7801 gcaggatgtc tccgtcgaag tattccttct ctacttccac tctaacgatc atgtcgtcgt
     7861 tgaagccttc cctttccaag ataatctgaa agtttcttcc gactcccggt atctccatga
     7921 gctttttctc gacctggatc gggaagatgt tgacgccctt gatgatcagc atgtcgtcgg
     7981 ttcttccctt gatcctctcg atccggcggt gcgttcggcc gcagggacaa gggccgggca
     8041 tcacccgagt aaggtccttc gttcggtaac ggaggatcgg cattccctcc ctcgtcaagg
     8101 ttgtgaggac gagttcccct tcttcgcctt caggtaacgg tttcagcgtg tcggggtcga
     8161 tgatttccat gagaaaatgg tcctcccaga ggtgcagccc gttctggaaa gggcattcga
     8221 aggccacgcc gggaccgttc atttcggaga gtccatagga gttgaacgcc ttcaacccgt
     8281 acagttcctc tatcttcagt cgtgttttct cggagtgcgg ttctgcgccg agaaaggcga
     8341 acttcagtct cgtgtccttc ttagggtcga ttttcatttc ctcaaaggtg gtgtaaagat
     8401 gcagggcata gctcggtatg atatggatcg tcgtcgtgtc gaaatcgcgc atgagctgga
     8461 tctgccgttt gctgttaccc gtccctgccg ggataaccaa cgctccgacc ttttccgccc
     8521 catagtgaaa ccccaggccg ccggtaaaga ggccgtaggt catcatgttc tggaaaacgt
     8581 cgctcctcct gaccccggcc atgtacatac acctcgcgat gaggtcggcc catccttgga
     8641 tgtctttgag ggtgtgaaag acgacggtcg cccgtcccgt agtgccggat gacgagtgca
     8701 tcctaacgag gtcgtccttg gggacggaga gaaacccgta aggccagtgt tcgcgaaggt
     8761 cgtcttttgt ggtaagcggt atggatccaa ggtcttccag tgaaccgagg gaatccgggt
     8821 cgaacttgat ttcgttgaac ttctccctat aatgagtcga ggccttcgcc cggttgagcg
     8881 tctccttcaa cctggagagc tgcagcgcct cgagttcccg ccggggcatg gcctcgattt
     8941 ctttttccca gtacatgttt gatgaacact cctatggaga atttccatcg atttctacaa
     9001 cccgccatga aatttatcaa gctaaatctg cccgtaggaa taccgtttgt cttcgaccgc
     9061 cgaaagggtt atatcgaggc atcatcgatt acggccaagg aatgttgacg agaggatact
     9121 caggaagacg agaatgaccg gcatagtgct gtgcggcggc aagagcagca ggatgggcac
     9181 gaacaaggcc ttccttgagg ttgagggcga acgccttatt gatagggtgg tatgggttct
     9241 tgggcggcat tttgcggagg tcgtcctggt gacgcggact cctactgatt atcaaagcat
     9301 agatgccgtt atcgttaagg acatgattcc cgaagggggt gtcctgggag gcatctatac
     9361 ggggttgttt ttctcctctg cggagattgc attcgtcgcc gcctgcgata tgcccttttt
     9421 ggatgatcgg ttcatcgatc acatgattcg gagggcagaa aactatgaca tagtcgtccc
     9481 aatgacaaga gagggctttc agcccttgca cgcgatctat tcgcggcgat gtctcccgga
     9541 aatgttgcgg atgatcgag
//
LOCUS       DAOTMX010000021         9548 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_6604_length_9548_cov_4.198567, whole genome shotgun sequence.
ACCESSION   DAOTMX010000021 DAOTMX010000000
VERSION     DAOTMX010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 9548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9548
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6604_length_9548_cov_4.198567"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="P5573_01190"
     CDS             complement(<1..640)
                     /locus_tag="P5573_01190"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF023169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRT26714.1"
                     /translation="MSSFKKIVLGLFFFLLSPGCAFATTEILNIRHWVAPENTRIVID
                     TDEEADTVVSRTDQTITLDFKDTEISDTVPSEEILNKPGIKKIVVVPLPDNSVRVQLQ
                     LADYVETRVFKLKAVADKSFRVVVDIALPEMEKKEREERKQQKILKKDKIIVIDAGHG
                     GDDPGAVGLRKTKEKEVVLKIAQRLQKILDKKQGYRAFLTRRGDYYPSFKKRI"
     gene            800..1672
                     /gene="metF"
                     /locus_tag="P5573_01195"
     CDS             800..1672
                     /gene="metF"
                     /locus_tag="P5573_01195"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HRT26715.1"
                     /translation="MKIRELFRQEVLTLSFEVFPPVREGNLESLYRAIGELKSLKPDF
                     FSVTYGAGGSTRDRSLEIASKVRNDYGQEVMAHLTCVQSTKDEIAGILDELRDSGIEN
                     ILALRGDPPLGQEKSVKTEGGFGYANELVEFIRSRGDFCIAVAGYPEGHIEAPSLEED
                     LRNLKRKVDAGADFIISQLFFDNDVFYRFRDRAWKAGIRVPLLPGVFPVMNYKQMLRC
                     ISLSNASVPKKLAEKMDSLQDKPEETEKYGVEYAVGQAEDLIRNEVQGLHIYSMNRSG
                     PVIRIIEQLDLPGR"
     gene            1907..2353
                     /locus_tag="P5573_01200"
     CDS             1907..2353
                     /locus_tag="P5573_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HRT26716.1"
                     /translation="MGIKAQLESDIIAAAKAKDKLRLSALRMVKTAMHNKEIDLKRDL
                     TEPEMLQLLSSLAKQRADSIEQFRNGGRMDLVEKEEAELKIIKGFMPEQMSEKDIEEE
                     VGKAIAEVGAVGPKDMGKVMKALMPKMAGKADGKLVSELVKKRLSA"
     gene            2591..4378
                     /gene="dnaG"
                     /locus_tag="P5573_01205"
     CDS             2591..4378
                     /gene="dnaG"
                     /locus_tag="P5573_01205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HRT26717.1"
                     /translation="MGFVRSEAEEVKRRTNIVDVVSEYVPLKKSGVNYTGLCPFHKEK
                     TPSFSVNEDKQIFYCFGCGEGGDVFAFIMKINDMSFPEALRYVAGKVGISLSKEYRGV
                     GSSAGKSLREEILRVNGMAADYYAGNLLSVAGEEARVYLKRRGLREDVVRTFRLGYSL
                     PGWHNLNGYFEGRKVSSGLLEKAGLVIRRDDGAYYDRFRGRLMFPIEGVNGNVVAFGG
                     RSLGDEVPKYLNSPESPVYVKGRNLFGLSRAKEEIRRHNEAIIVEGYFDLLQLWNAGI
                     RNVVATLGTALTADQLSLLRRYTRNVVLLFDSDEGGRHAIERSLKLFVAEKIHAKIVI
                     LPEGYDPDTYVLKEGKEALAGLISKSQSMIDYYIDHIIGAKKGLEDRLETLGRAVGFI
                     VQVDDVKLRDIMMTRVSERLRISEEILRTEIRKAISSKEGKPRERGEVLQPLRSRKAD
                     PIELNFIHLLVEHPEWHRAVQEEKVLDNLLSSELRALGQYILEESRRGEKIDVNMVVD
                     RVDDEEMKKKLFRRFMEGVYHDAGIAEKEFTDTVLKIKRRWLKQRRKLLEMELVKAQE
                     DKNRCDLLLGEQQRLVEEEKELSFSIREH"
     gene            4393..6135
                     /gene="rpoD"
                     /locus_tag="P5573_01210"
     CDS             4393..6135
                     /gene="rpoD"
                     /locus_tag="P5573_01210"
                     /inference="COORDINATES: protein motif:HMM:NF004208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HRT26718.1"
                     /translation="MHSDEIKKLISLGEEKGFLTYDDVNDILPSDVTSPEEIDDIIML
                     FGEKNIDIIDTDKGEKIVLKKTAEEIAEGKEEEEDLSALLTTAGKTGDPVKMYLREMG
                     VVSLLNREGEIEIAKKIEEGEREVMEAVFSVATSVKDVIDIAKKLRSEDLRIKNVVDN
                     LEDEDGFVEEDMHRERVLELIDKIARIEKRNDTQRKKIADRFSTAEEKKAAKQELAKN
                     TKKIVELSREIKFSKKQINRMVSRLKDAANEIKKAEEKVLLCKDRTGMTIKEMEKTVA
                     EMNRNPDKAGKIARELRVSIKRMKECEAAIKECRIVTRKVTAGIGLPVPLLKNVVARI
                     EEGETKANEAKKKLIEANLRLVVSIAKKYTNRGMQFLDLIQEGNIGLMKAVDKFEYQR
                     GYKFSTYATWWIRQAITRAIADQARTIRIPVHMIETINKLIRTSRYLVQELGREPNPE
                     EIAEKMEFPLEKVRKVLRIAKEPISLETPIGEEEDSHLGDFIEDKKIMSPSDAAISMD
                     LAKQTRKILATLTPREEKVLRMRFGIGERSDYMEEAIDDLGATLERTRQIEANALKML
                     RNPSRRRELKMVVK"
     gene            6254..6326
                     /locus_tag="P5573_01215"
     tRNA            6254..6326
                     /locus_tag="P5573_01215"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6287..6289,aa:Ile,seq:cat)
     gene            6480..7199
                     /locus_tag="P5573_01220"
     CDS             6480..7199
                     /locus_tag="P5573_01220"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HRT26719.1"
                     /translation="MKEDLQHLISLQRIDASLASASIQKQEVPGRIARLDQDYDFLRE
                     RADEGKRRLDELLKEHRAKEDSLKMGIEHLKKTKERLLEVKTNKEYQAMLKEIDGIVK
                     KNEEIEDEIISKLEDIDAARNNLKVREAELAEFRLRYDKERKDLEGEMSRIDGETTRL
                     MKDVERVRGKIDSDILKQYDRIKAKRNGSAVAPVWKGVCEGCHMNLPPQMCNELQKSA
                     EIMQCPFCSRIIYWDDRGENG"
     gene            7192..7611
                     /locus_tag="P5573_01225"
     CDS             7192..7611
                     /locus_tag="P5573_01225"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HRT26720.1"
                     /translation="MADTPFRLYTDGACRGNPGLGGIGVVFKDEQGNIVGTIKGFIGS
                     CTNNEAEYQALIAGLKEAVRRGYSRLSIYMDSELVVKQINGAYRVKSKNLSEYFQEVK
                     DLLSAIGNYRVFHIGRNENKLADQLANEAIDEHEGSL"
     gene            7630..8081
                     /gene="rnpB"
                     /locus_tag="P5573_01230"
     ncRNA           7630..8081
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="P5573_01230"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            8206..>9548
                     /locus_tag="P5573_01235"
     CDS             8206..>9548
                     /locus_tag="P5573_01235"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HRT26721.1"
                     /translation="MHGLVKEGKYLEAIAECQAIVEHAQQDDTKATAFLMIGDIYSYY
                     LDDYDAALRYYDLVKTEYPKSNASANAFFNSGMILYEKGRFKEALLQFNVYLKNYPQG
                     TRRDTASYMVEACSREIPLTVAKEDKPVGPAARDIDIVRVLLAASAKPVVITVTSSVE
                     VMDANEKRTLGRLATGDKVVGVSNGMLMIDGQPVGDGEIILICAPGEKLKLDGKRYRG
                     KVKLKKTALGGVAAVNVLRTEEYLYGVVPREMLPSWPLEALKTQAVAARTFVLYQKDK
                     NRNDDYDVTATTLSQVYGGFDCETSETRQAVDCTKGEVITHGGRLILAYYHANSGGMT
                     EDAGNVWTTDLPYLKGVIDPFSEGAPDCRWSVLLRLKDIARSLSENGIRVNVVHDVTP
                     VGVSPSGRVTRVRVVHDRGETILRSNDFRIKLDARLVKSTLFVAVREGDVIRFDG"
BASE COUNT         2580 a         2087 c         2789 g         2092 t
ORIGIN      
        1 gaattctttt cttgaaagag ggatagtagt cgccgcggcg cgtcaggaag gcccggtagc
       61 cctgtttttt gtccaaaatc ttctggagcc tttgggcgat cttgagaacg acctctttct
      121 ccttcgtctt tctcaatcct actgccccgg ggtcgtcccc gccatgaccg gcgtcgatca
      181 cgatgatctt atctttcttg agtatttttt gttgttttct ctcttctctc tccttttttt
      241 ccatctccgg gagcgctata tcgacgacga cgcgaaaaga cttgtcggcg acggccttca
      301 gtttaaacac gcgtgtttca acatagtcgg ccaactggag ctgaacccgg acagagttgt
      361 cgggaagggg tacgactact atttttttga tacccggttt gttcaggatc tcttccgacg
      421 gaacggtatc gctaatttcc gtgtccttga aatccagggt gatcgtttgg tccgttctgc
      481 tgactacggt gtcggcttct tcgtccgtgt cgatgactat gcgggtgttt tccggagcga
      541 cccagtgccg aatgttgagt atctcagtag tcgcgaaggc gcatcccggg ctgagaagaa
      601 aaaaaaacag gccaagaacg atttttttga aactggacat ctgaggtatt cttcctttat
      661 tttaaaaact ttaacagatt aggcggtcta ttacaaccag taggatagaa aactggttga
      721 caactggcgt tcttagtgac attttcccgc attgtaaaaa ggtctggaag cggcatcaat
      781 aggagtcggg aaggatcaga tgaagatcag ggaacttttt cgtcaggagg tgttgactct
      841 ttccttcgag gtctttcctc ccgtgaggga agggaacttg gagagtcttt acagagctat
      901 cggagagctc aaaagcttga agccggattt tttctccgtc acctacggag cgggtggtag
      961 cacccgagac aggtctcttg agatcgcttc gaaagtcagg aacgactacg gtcaagaggt
     1021 catggctcat ttgacctgcg tacaatcgac gaaggacgag atagccggga ttctcgatga
     1081 actgcgggat tcgggcatcg agaacatcct tgcccttcgg ggggacccac cgttgggaca
     1141 ggaaaaatca gtcaagacag aaggcgggtt cggctatgcg aacgaattag tcgaattcat
     1201 caggtcgagg ggtgactttt gtatcgccgt ggccggctat cccgaaggtc acatcgaagc
     1261 gccgagcctc gaagaagacc ttcgcaacct gaagaggaaa gtcgacgctg gtgcggactt
     1321 tatcatttct cagctattct tcgataatga cgtcttctac cgcttccgtg acagggcctg
     1381 gaaggccggc atccgggttc cgcttctgcc cggtgttttt ccggtcatga attacaaaca
     1441 gatgctgcgg tgcatttccc tctccaatgc gagcgttccg aaaaagcttg ccgaaaagat
     1501 ggatagcctt caggataaac cggaagagac ggagaagtat ggcgtcgagt acgcggtcgg
     1561 ccaggcggaa gacctgatcc gtaacgaggt ccaagggctc catatctaca gcatgaaccg
     1621 gagcggaccc gtcatccgga tcatcgagca gcttgacctg cccgggcggt aatgacctct
     1681 gatccttgct ggatatcagt gccagcgttc ggtaacacct atttatgatc caacgatcct
     1741 tttttgacca cctcctgggt aacatttaat ttgaggcaat tcgtggagct gaaatagatt
     1801 gacaagaaaa aaaagttttc tataattaat cgttttcgta ttgtattttt gtcgtacttc
     1861 taaaaatacc tgatccatgt aagggtcaga caggtgaggt tctaccgtgg gtataaaagc
     1921 gcaactcgaa agtgacatca ttgcagccgc taaggcgaag gacaaactga ggctttccgc
     1981 tctccggatg gtcaagacgg cgatgcacaa caaagagata gacctgaaga gggatctgac
     2041 ggaaccggaa atgttgcagc tcctctcatc cctcgctaaa caacgcgctg attccatcga
     2101 gcaattcagg aatggcggca ggatggacct ggttgagaag gaggaggcgg aacttaagat
     2161 tatcaaaggt tttatgcctg agcagatgtc cgagaaagac atcgaagagg aagtcggaaa
     2221 ggcaatcgcg gaggtaggcg ccgtcgggcc caaggatatg ggcaaggtga tgaaggccct
     2281 gatgcccaaa atggccggca aagcggacgg gaaactcgtc agcgaactgg tgaaaaagag
     2341 gctctctgcg tgagcatgtc tgctctgatg taagatttgg gactccgagg tcgtcgagta
     2401 ccgacgagtt gtggttattg taaacattga ttgtattcct aatttctcgg gtggaattgc
     2461 ttaattgcct gtgttaaaag acgtagtttg ggtttttgcg gctctgtcct ttctacgatg
     2521 agagcttcgt tacatccggg ctgggaaagt ctttctgttg tgctcaagct ggatgatggg
     2581 gaaccaccct atgggttttg ttcgttctga ggcagaggaa gtcaagagaa ggacgaacat
     2641 cgtcgatgtc gtatcagaat atgttcctct caagaaatcc ggggtcaact ataccggtct
     2701 ctgtcctttc cataaggaaa agacaccttc tttctcggtg aatgaagaca agcaaatctt
     2761 ctactgcttc ggctgcgggg agggtggtga cgtattcgct tttatcatga aaatcaatga
     2821 catgagcttt ccggaggcgc tcaggtacgt tgcgggaaag gtcgggataa gtctttctaa
     2881 ggaatacagg ggcgtaggga gtagtgccgg gaagagcctg cgggaggaaa ttcttcgggt
     2941 taacggaatg gcagcggact actatgccgg gaatcttttg tccgttgcgg gggaagaggc
     3001 cagggtttac cttaaacgaa ggggtctccg ggaggatgtg gtcaggacat tccggctggg
     3061 atattccctc cccggatggc acaatctcaa tggttatttc gagggtagga aggtttcctc
     3121 gggattactc gagaaggccg gcctcgtgat aagaagagat gacggtgctt attatgaccg
     3181 cttcagggga aggttgatgt tcccgatcga gggtgtcaac ggaaacgtgg tcgcctttgg
     3241 ggggaggtca ctgggggatg aagtccccaa gtaccttaac tcgcccgaat cccccgttta
     3301 tgtcaaaggg aggaacctct tcggcctgag cagggcgaaa gaagaaatcc gtcgtcacaa
     3361 cgaggcgata atagtagaag gatactttga ccttctccaa ctctggaacg ccggcataag
     3421 gaacgtcgtt gccacccttg ggacggcact gaccgcggat caactcagtc tgttgcgccg
     3481 ttatacgagg aacgtcgttc ttctgttcga ttccgacgaa gggggaaggc atgcgattga
     3541 acgaagcctg aagctttttg ttgcggagaa gatacatgca aagatcgtaa tccttccgga
     3601 aggttacgac ccggacacct atgttttgaa ggaaggaaaa gaggccttgg cgggtctgat
     3661 ctccaaatca caatccatga tagactatta catcgaccat atcatcggag ctaaaaaagg
     3721 tttggaagac cggctggaga cgctcggccg ggccgtaggt ttcatcgttc aagtagatga
     3781 tgtaaaatta agggatataa tgatgacgcg ggtctcagag cgactcagga tttccgaaga
     3841 aattcttaga accgagatcc gcaaggcgat ttcgtcaaaa gagggcaagc cccgggaaag
     3901 aggcgaggtt ttgcagcctt tgaggtcccg gaaagccgac ccgatcgagc tgaatttcat
     3961 ccatcttctc gtggagcacc ctgaatggca tagggcagtt caggaggaaa aagttctgga
     4021 taatctgctg agcagtgaac tgagggctct gggccagtat attcttgaag aatcccggag
     4081 aggagagaag attgacgtca atatggtcgt cgaccgcgtc gacgacgagg agatgaaaaa
     4141 aaaactgttc agacgtttca tggagggggt ctatcatgat gcggggatcg ccgagaaaga
     4201 gtttacggat acagttttga agataaagag acgatggctt aaacagcgaa gaaaattgct
     4261 ggagatggaa ctcgtaaagg cacaggaaga caagaaccgt tgcgacctcc tgcttggcga
     4321 gcagcagcgg ttggtcgagg aagaaaaaga attatcattc agtatcaggg agcattaaaa
     4381 atgcgaaaga gtatgcattc agacgagata aagaaattga tttccctggg agaggagaag
     4441 ggattcttga cttacgacga cgttaacgac atcctgcctt ccgatgtgac ttctccggaa
     4501 gagatcgacg acatcatcat gctcttcggc gagaagaaca tcgatatcat agacacggac
     4561 aaaggtgaaa agatagtcct caagaagacg gccgaggaaa tcgccgaagg gaaggaggag
     4621 gaagaagacc tctccgccct tctgaccacg gcgggaaaga cgggcgatcc ggtgaagatg
     4681 tacctgcgcg agatgggcgt agtatctttg ctcaatcgcg agggcgaaat tgagatcgca
     4741 aagaagattg aggaaggtga aagagaagtc atggaagcgg ttttcagcgt tgccacttcc
     4801 gtgaaggatg tcatcgacat cgccaagaag cttcgtagcg aggacctgcg gatcaagaat
     4861 gtcgttgaca acctcgagga tgaagacggg ttcgtcgagg aggatatgca ccgggaaaga
     4921 gtcctggaac tcatcgataa gattgcccgg atcgagaaga ggaacgacac gcagaggaag
     4981 aagattgccg ataggttctc gactgcagag gaaaaaaagg cggcaaaaca ggagttggcc
     5041 aagaacacca agaagattgt cgagctttcc cgggagatca agttcagcaa gaagcagatc
     5101 aaccgcatgg tgtcaagact gaaagacgcg gcgaatgaaa tcaagaaggc cgaggagaaa
     5161 gtcctgctgt gtaaggatcg gacgggtatg accatcaaag aaatggagaa gaccgtcgct
     5221 gagatgaacc ggaatccgga taaggcaggc aagatcgcgc gagaattgag ggtttccata
     5281 aagcggatga aggagtgcga ggcagcgatc aaggaatgtc ggatagtcac caggaaagtt
     5341 acggcgggaa tcgggcttcc ggtccctctt ctgaaaaacg tggttgcaag gatagaggaa
     5401 ggagagacga aggctaacga ggccaagaaa aagctgatag aagccaatct caggcttgtg
     5461 gtcagcatag cgaaaaaata taccaacagg ggcatgcagt ttctcgacct catccaggaa
     5521 ggcaacatcg gtctgatgaa ggcagtcgat aagttcgaat atcagcgcgg ctacaagttc
     5581 tcgacttatg cgacatggtg gatacgccag gcgatcacca gggcgattgc cgaccaggcg
     5641 cggacgatcc ggattccggt ccatatgatc gagaccatca acaagctcat caggacatcc
     5701 cgctatttag tgcaggaatt aggccgagaa ccaaacccgg aagaaatcgc ggaaaagatg
     5761 gagttccccc tcgaaaaggt cagaaaagtc ctgaggattg ccaaagaacc tatttctctc
     5821 gagacgccca taggcgaaga ggaagacagt catctgggtg atttcataga agacaagaag
     5881 atcatgtcac cctccgacgc cgccatcagc atggaccttg ccaaacagac gcgaaaaatc
     5941 ttggcgacac tgacaccgcg ggaggaaaag gtcttgcgga tgcgtttcgg gatcggcgag
     6001 cgctccgact acatggaaga ggccatagac gacttaggcg cgacactgga gcgcactagg
     6061 cagatcgagg cgaatgcact gaaaatgtta agaaatccgt caaggcggcg ggaactcaag
     6121 atggtcgtga aatgatcttg tcaaaaattc ttgacaaaaa ggatgaatga gaattacaag
     6181 aacaactcac tcgttgcgcc gtgagataca cggtataaac gatgatgatg cagtgagtgc
     6241 gatgttcatg aacgggccca tagctcaact ggaagagcca ccggctcata accggtaggt
     6301 ccctggttcg aacccaggtg ggcccactat caagcgcgaa ttgcgaggtc ctggttcaag
     6361 atgcctttaa aagcatatac tgtacaatca ggatgcccgt ctgggtatgg tctgaaatgc
     6421 acctgtaagg gtgcattttt tttagccttt gagtcaaatt aagaacagga ggaagatgat
     6481 tgaaagagga tcttcagcat ttgatttcgc tacagaggat tgacgccagc ctggcaagcg
     6541 ccagtatcca gaagcaggaa gttccaggaa gaatagcccg ccttgaccaa gattatgact
     6601 ttctcaggga aagggcagat gaaggaaaac ggcggctcga tgaactcctg aaggaacaca
     6661 gagcgaaaga agacagtctg aagatgggca tcgagcatct aaagaaaact aaagagcgcc
     6721 tcctggaagt gaagacgaac aaggaatatc aggcgatgct caaggaaatc gacggcatcg
     6781 tcaagaagaa tgaagaaatc gaggatgaga taatttcgaa gctcgaagac atcgatgccg
     6841 cgaggaacaa tctcaaggta agagaagcgg aactggcgga attccggctc cggtatgaca
     6901 aggagaggaa agaccttgag ggtgagatga gcaggatcga cggtgaaacc acccgcctga
     6961 tgaaggatgt agaaagggta agggggaaga tagactctga tattctcaag cagtatgata
     7021 gaataaaggc aaagcggaac ggcagtgccg tggccccggt ctggaaaggg gtgtgtgaag
     7081 gttgtcacat gaacctgcct cctcagatgt gcaatgaatt gcaaaaatcg gcagaaatca
     7141 tgcagtgccc tttttgcagc cggatcattt actgggacga caggggagaa aatggctgat
     7201 acgccgttca ggctttacac cgacggtgca tgccggggta accctggcct tggcgggatc
     7261 ggggtcgttt tcaaggatga acagggcaac atagtcggta cgatcaaggg atttatcggt
     7321 tcctgcacca acaacgaagc ggaataccag gccctcattg ccgggttgaa ggaggcggtg
     7381 aggcgcggct actcccgact ttccatctat atggattctg aactggtggt gaaacagata
     7441 aacggcgctt accgggtgaa gagcaaaaat ctatccgaat attttcagga agtgaaagat
     7501 cttctctccg ccatcgggaa ttacagggtt tttcatatcg gacggaacga gaacaagctg
     7561 gccgaccagt tggccaacga ggcaatagac gaacacgaag gaagtcttta gctgaacgct
     7621 ctttgatgtt ggagtagacg agatgatcgc cggtccgctt ggggtgggcc ggaggaaagt
     7681 ccgagctcca cagggcagga tggtcgctaa cggcgactgg gggtgacccc aaggaaagtg
     7741 ccacagaaaa gataccgccc accggctgca agctgggatt cgggtttgga atcgccgagt
     7801 ttcggttagt tttgaattct gaactctgcg gtgatgaccg cggtgggtaa gggtgaaaag
     7861 gtgaggtaag agctcaccag tttcctgggt gaccgggaaa gcttggtaaa ccccatccgg
     7921 agcaagacca aataggagga cgcttgaggg ctgcccgtcc gatgtcctcg ggtagagtcg
     7981 cttgaggcac cgggcaaccg gtgtcccaga ggaatgatca tcgcccttca acgggtgacc
     8041 gtgaaaggga acagaactcg gcttatcggc tgctccaaca tcaaacactc agtagaaacg
     8101 acgaactgat ctgagatgca tcgcgcaata ctttcaatca taaccctgtc gatctttctt
     8161 tgcatttctt ttggcttcgg cagcgaaacc gaagcgcctt ctcatgtgca cggcctggta
     8221 aaggaaggga aatacctgga agcaatcgct gagtgtcagg cgatagtgga acatgcccag
     8281 caggatgaca cgaaagccac cgcgttcctg atgatcgggg atatttacag ctattacctc
     8341 gacgactatg atgccgctct tcggtattac gaccttgtaa aaactgagta ccccaaaagc
     8401 aatgcctccg ccaacgcctt tttcaattct ggtatgatcc tttacgaaaa gggcagattc
     8461 aaggaagcct tgttgcagtt taatgtttac ctgaaaaatt atccgcaggg gacaagaaga
     8521 gacacggctt cttacatggt cgaggcttgt agccgggaga tcccgctcac ggtggcgaaa
     8581 gaggacaagc ccgtgggtcc tgcagcgagg gacatcgata ttgttagggt ccttctggcg
     8641 gcatcggcaa agccggtcgt gattacagtt acttcatcag tggaggttat ggatgcgaat
     8701 gaaaagagga cgttggggag attggctacc ggagacaagg tcgtaggtgt atcgaatggt
     8761 atgctgatga ttgatggaca gcccgttggg gatggagaga tcatacttat ttgcgcccct
     8821 ggagaaaagc tgaaactgga cgggaaaagg taccgcggga aggtaaagtt gaagaagacg
     8881 gcattgggag gtgtggctgc ggtcaatgtc ttgcgtacgg aagagtacct ttacggcgtt
     8941 gttcccagag aaatgttgcc gagctggccg ttggaggcat tgaagaccca ggctgtcgcg
     9001 gctagaacct tcgttcttta ccagaaggac aagaaccgga acgacgatta tgatgtaact
     9061 gccactactt tgtctcaagt ctacgggggt tttgattgtg aaaccagtga gacgcggcag
     9121 gcagtagatt gtacaaaagg ggaagtcatt actcacgggg gcaggctgat tctcgcatac
     9181 tatcatgcca atagcggcgg catgacggag gatgccggaa acgtctggac aaccgacctt
     9241 ccttacctaa agggggtgat agaccccttc agcgagggag cgcccgattg tcggtggtcc
     9301 gtcctcctcc gcttgaagga catcgcgagg tctctttcgg aaaatggtat tcgggtgaat
     9361 gtcgtccatg acgtgacccc tgtcggggtt agtccctccg ggagggttac gagggtcagg
     9421 gtcgtgcacg acaggggcga gacgatactc cgcagcaacg atttcaggat taagttggat
     9481 gcgaggctgg ttaaaagcac tctcttcgtg gctgtgagag aaggcgatgt cattagattc
     9541 gacggaag
//
LOCUS       DAOTMX010000022         9483 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_6649_length_9483_cov_3.448239, whole genome shotgun sequence.
ACCESSION   DAOTMX010000022 DAOTMX010000000
VERSION     DAOTMX010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 9483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9483
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6649_length_9483_cov_3.448239"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="P5573_01240"
     CDS             complement(<1..315)
                     /locus_tag="P5573_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRT26722.1"
                     /translation="MPNSIPACERYVTVIGGGLAGCEAAWQILKRGLRVKLYEMRPTV
                     FTPAHKTSGLAELVCSNSLRSNELDRAAGLLKEEMRLLDSLVMRAAGESAVPAGTALA
                     VDR"
     gene            complement(336..2594)
                     /gene="topA"
                     /locus_tag="P5573_01245"
     CDS             complement(336..2594)
                     /gene="topA"
                     /locus_tag="P5573_01245"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HRT26723.1"
                     /translation="MKRSLIVVESPTKVKTIKKYLSSEFDVRASVGHIKDLPKSKLGI
                     DIKNDFKPTYQVLETKKKVITELKKAAAVADTIYLAPDPDREGEAIAWHIAEEIDGNK
                     TIYRVLFNDLTKNTMLTALQHPQKLNLDKYEAQQTRRILDRLVGYQVSPILWEKVRRG
                     LSAGRVQSVAVRMVCDREEEIRNFVPEEYWNITALLEGSEPPPFEARLIKVDGKKASI
                     SNSSESEAVLKRLESAPFIVACVEKKEARRNPAPPFTTSKLQQEASRKLRFSAKKTMS
                     VAQKLYEGIELGKEGPVGLITYMRTDSVRIAEEALSEVRRYILEAHGKEYLPAKPRVY
                     KSGRNAQEAHEAIRPASLNYRPLDIKPYLTNDQFRLYQLIWNRFVASQMNPAVYDRTT
                     IDIEAANTLFRAQGDVMKFPGFMTLYTEGKDDNGEENGAEKALPDVKVGERLKLLSLN
                     PEQKFTEPPPRYTEATLVKELEEKGIGRPSTYAAILSTIQERDYVKLDGGRFHPTELG
                     KLVTDLLVKNFPTILDVAFTAGMENELDMIEEGKLKRIESLQKFYSTFEKYLKKAKSE
                     MRNVKKEETPTGLVCEKCGSPMVIKWGRNGRFIACSSYPKCRNTMNFQEDENGAVTQK
                     PPAATETDIECPDCGRKMVVKQGKFGSFLGCSGYPECRFTTSLSLGVKCPVEGCGGEV
                     VEKRSRKGKVFFGCSRYPDCSYATWDKPIAEPCPECGAPFILEKYNSKKGRYRVCSNQ
                     SCTYRTDKEPAS"
     gene            complement(2704..3822)
                     /gene="dprA"
                     /locus_tag="P5573_01250"
     CDS             complement(2704..3822)
                     /gene="dprA"
                     /locus_tag="P5573_01250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HRT26724.1"
                     /translation="MDENNLKCWLALKLVEGLGSVGVKNLLSAFGTPRRVLEAPLEAL
                     KSVPGIKEKTAKIIHRFNRWEQVERELALAREKGVSLVTVEDASYPRLLLDIYDRPPL
                     LYVKGNLVEDEATIAVVGSRAATTYGKFLTERLCRELAQYGFTIVSGMARGIDAAAHR
                     GALSANGRTIAVLGCGIDVYYPLENKKLYDEIAANGAVVTEYPFGTQPHAANFPMRNR
                     IISGLSLGVVVVEATDKSGSLITARTALEQGREVFAVPGAVDSPGSKGTHRLIKEGAK
                     LVEGIDDIVEELQPHLRKTSPTPQRPEQADARRRVDEKPLGERERTILNLLSEGAVDV
                     DTLIDKTGLDTNEVLTILLGLELGGYVEQKPGKVYNRI"
     gene            4022..4831
                     /gene="murI"
                     /locus_tag="P5573_01255"
     CDS             4022..4831
                     /gene="murI"
                     /locus_tag="P5573_01255"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HRT26725.1"
                     /translation="MKIELVHPIGVFDSGIGGMTVVRALMERLPFENIIYFGDTARVP
                     YGIRSVETITEYATQIANFLLKQNVKLLIIACNTIASVANERIQEMSPVPVLDVIDAG
                     ARIAVAETRNKIVGVIGTPATINSNAYARAINRYDPEIRVFSQACPLFVPLVEEGWLD
                     HPVTRLTAQEYLKPVIAERIDTLVLGCTHYPLLKPLLQEIVGEGIRLVDSAEAMAAVT
                     ANLLSEKGLANPVRLPPQYRFYVTDVPYRFQTVGERFLGRTLANVEVVKLQ"
     gene            complement(4912..5412)
                     /locus_tag="P5573_01260"
     CDS             complement(4912..5412)
                     /locus_tag="P5573_01260"
                     /inference="COORDINATES: protein motif:HMM:NF015147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HRT26726.1"
                     /translation="MEEKRQYEKVTVRTGLLFLIVLFVAFASCLNRVVEKPTFILREV
                     TISPRSLLEMNLLIGLDVQNPNRFDLTIKSFEYRVYLNNEEIGSGRLESEVLVPASST
                     TAVQAPVAANFKNLGGSLKALVMESDLPYKIEGRAEIEAIFGSLTFPFSKEGRLNPKQ
                     HEGERK"
     gene            complement(5417..6031)
                     /locus_tag="P5573_01265"
     CDS             complement(5417..6031)
                     /locus_tag="P5573_01265"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HRT26727.1"
                     /translation="MKPVRTIEKLKSGAETTIVAIGDSLTQGWMVRKGFLDFLAEMLR
                     QAYPESRFHIVNSGIPGDTAEGGLYRLRADVLDHNPDCVFIQFALNDAFCGYSAERFG
                     EYIQRMVDEIREDGDAEIVLVTSSYVGNNRETRFVEDFYDELENLATVNGLPVARVHS
                     HWKKRIGEDVEFRTLVQADLVHPTTAGYKLMAEAVMQLFERERM"
     gene            6106..7086
                     /gene="mtgA"
                     /locus_tag="P5573_01270"
     CDS             6106..7086
                     /gene="mtgA"
                     /locus_tag="P5573_01270"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HRT26728.1"
                     /translation="MPAAPPMGVLTITGLEGRRLGAGGVRLVSGMRSRLRLKNIVLSV
                     ATFVFAAMLLNIGYYLVFPDVSKLKKVNPRKTSFMEYREKEWQAQGKKRKIRYVWTPL
                     SQISPYVVKAVLIAEDDKFWRHEGFDFEAMKKALEKDIRKGKFRAGGSTISQQLAKNL
                     YLTPSRNPVRKVKEAILTWRIERNLTKRRIIELYLNVAEWGEGIFGIGVAAWHYYGKP
                     ASALGPMEAARLAAVLPNPRHWSPTGASSYVESRSQRIYQIMVRRGIVIEDYDEVMNT
                     PQEETVDEKPEATTTRESPEGKETPGTEGTSLSSPRKDEMEEQGGDATEE"
     gene            7145..8764
                     /locus_tag="P5573_01275"
     CDS             7145..8764
                     /locus_tag="P5573_01275"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HRT26729.1"
                     /translation="MSAIFLFLTGLSLFLYGMVELSALMRERFNNARLRENFKYAVRK
                     PVYGLVTGVVATVLFQSSTATSLLVVGMVSAGLMSFYRSLSILLGADIGTVFTVQLVV
                     WKVTDISPLLVIAGGLLWALGKDRWKPLGEGVFYFGLMFFGLSIVSGVTAPLKDNPDV
                     VRLLRETGGPLTGLLAGVLIAALVHSSAIPISILVILAQYQMVTTESALPIVFGANVG
                     TAVTAIMASIVTNVDGRRSALSHLLFKVFGTAVCFSVIPVFVGFLKLLSADTAQQIAY
                     GHLLFNLVTAGCFIFLLRPFSRLVEYLLPGKADTLPVWPEFLDARSLAVPEEALECVR
                     KELHREMTLARSMFLNSVRLIDDYGERRRKDIMYVELVVDNLRNEVSEYLRKVSAGEL
                     SAALSKRLFSYAAMVDDIERIADHSVNIVEFSRCKHLRNAKFSPMAEADLAEIIRLID
                     ENLRASLSLLEERDEDKIRDISLREDEVDRKVKEARERHLERFHKRICQAEAGPIFVE
                     LLVNLERISDHCQNIADYVAELEGTPSLPAA"
     gene            8964..>9483
                     /locus_tag="P5573_01280"
     CDS             8964..>9483
                     /locus_tag="P5573_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRT26730.1"
                     /translation="MGNNTKSPYFSGASRYVLDDELARIVNISMALEMPLLLKGEPGT
                     GKTMLAHAIAESLDMPLIVLNVKSSMKLVDALYQYDTLTRLNDSRFGDSKRDVSNIED
                     YIRMGKIGQAFVSEVRTVLLIDEIDKADTDFQDDMLDVLDQMQFDIIEVDKTVSAKHR
                     PVIIITSNAKKDL"
BASE COUNT         1889 a         2635 c         2719 g         2240 t
ORIGIN      
        1 acggtccact gccagggccg tccccgccgg aacggccgat tccccagcgg ccctcatgac
       61 cagagaatcc agcaaccgca tctcctcttt cagcagtccg gccgcgcggt cgagttcgtt
      121 cgacctaagg gaattgctgc aaacaagttc ggccaagccc gatgttttgt gcgccggagt
      181 gaagaccgtc ggcctcattt cataaagttt cactctaagg cctctcttga ggatctgcca
      241 ggccgcttcg cagcccgcca ggccgccgcc gatcacagtc acgtatcttt cacaggcagg
      301 tattgaatta ggcaccgact ggtagtctgt ttctatcagg acgccggctc cttatccgtc
      361 ctgtacgtac aagactgatt gctgcagacc ctgtagcgtc ccttcttcga attgtatttt
      421 tccaggataa aaggagcacc gcattcaggg cagggctcgg cgatgggttt gtcccacgtc
      481 gcatagctgc aatccgggta cctcgagcaa ccgaagaaga cctttccttt tctggaacgc
      541 ttctcgacga cctcaccgcc gcagccttcc acgggacact ttacgccgag gctgagggac
      601 gtcgtgaacc ggcattcggg gtagcccgaa cagccgagaa aactgccgaa ttttccctgc
      661 ttgaccacca tttttcggcc gcaatcgggg cattctatgt cggtttcggt cgccgcgggc
      721 ggtttctggg tgacggcgcc gttttcgtct tcctgaaaat tcatggtgtt gcggcacttc
      781 ggataactcg agcaggctat gaacctcccg ttacgtcccc atttgatgac catcgggctg
      841 ccgcattttt cacagacgag gcctgtaggc gtctcttctt ttttcacgtt acgcatctcc
      901 gatttggctt ttttgagata cttctcgaag gtggaataga atttctgaag gctctcgata
      961 cgttttagtt ttccttcttc gatcatgtcg agctcgtttt ccatgcctgc cgtgaaagcc
     1021 acgtccagga tggtaggaaa attcttcacc agtaggtcag tgaccaattt gccgagctcc
     1081 gtcgggtgaa accgacctcc gtcgagcttg acgtaatccc gttcctggat tgtgctgagg
     1141 atggcggcgt aggtgcttgg cctgccgatt cctttctcct cgagctcctt gacgagcgtc
     1201 gcctcggtgt agcgcggcgg cggctcggtg aatttctgct cgggattgag cgagagcaac
     1261 ttcagccgct cgcctacctt tacgtccggc agcgcctttt ccgcaccgtt ttcttccccg
     1321 ttatcgtcct tcccttcagt gtagagggtc atgaagcccg ggaacttcat gacgtcgccc
     1381 tgggcgcgga atagggtgtt tgccgcttcg atgtcgatgg tggtccggtc gtagacggcc
     1441 gggttcatct gactcgccac gaagcggttc cagatgagtt ggtagagacg gaactggtcg
     1501 ttcgtgagat aaggttttat gtctaagggc cggtaattca acgacgccgg ccggattgcc
     1561 tcatgggcct cctgggcgtt acgtcccgac ttgtataccc tcggctttgc cggaaggtat
     1621 tccttgccgt gggcctccag gatgtagcgc ctgacttcac tcagggcctc ctcggcgata
     1681 cggacggagt cggtcctcat gtaggtgatc aggccgacgg gaccctcctt gccgagttcg
     1741 atgccttcgt acagcttctg ggcgacggac atcgtcttct tggccgaaaa acggagcttc
     1801 cgcgatgcct cctgctgtag tttgctcgtc gtaaagggag gtgccgggtt tctccgggcc
     1861 tccttcttct ccacgcaggc gacgataaat ggagcggatt ccagtcgctt aaggacggcc
     1921 tcggattccg aggagttgga gatgctggcc ttcttcccgt cgaccttgat cagccgggct
     1981 tcgaagggag gaggttccga gccctctaaa agagcagtta tgttccagta ctcctcgggc
     2041 acgaaatttc tgatttcctc ttcccggtcg cagaccatcc tcaccgccac ggactgcacg
     2101 cggccggcgc tcaaccctcg ccttactttt tcccagagga tcggactgac ctgataaccc
     2161 acgagtcggt ccaggattcg ccgtgtctgc tgcgcctcgt acttgtcgag gtttaacttc
     2221 tgggggtgct ggagggccgt gagcatcgtg tttttggtca ggtcgttgaa cagcacacgg
     2281 tagattgtct tattgccgtc gatctcctcg gcgatgtgcc aggcgatcgc ctcaccttct
     2341 cggtcggggt cgggagcgag atagatggtg tcggcgactg ccgcggcctt cttcagttcg
     2401 gtgatgacct tctttttggt ctccaacacc tggtaggtcg gcttgaaatc attcttgatg
     2461 tcgatgccga gcttgctttt ggggaggtcc ttgatgtgac ctaccgatgc ccggacgtcg
     2521 aattcgctgc taaggtattt ctttattgtt ttgacctttg tcggcgattc aacgacaata
     2581 agcgaccgct tcattctttc tccttaaaag catattgcaa ggcgtatttc gaccggccgt
     2641 ttgtcggcgg ggccggggtg tgtacgtcgc ctctctcttt tttgtaaact tctctgcaag
     2701 ttcttatata cggttataga cttttcccgg tttctgttca acgtagccgc ccaattcaag
     2761 gcctagcagg atagtcaaga cttcattcgt gtcgaggcct gttttatcga tcagagtgtc
     2821 tacgtcaacc gctccttccg acagtaggtt caagatcgtc ctctcccgtt caccaagcgg
     2881 tttttcgtcg actcggcggc gggcgtcggc ctgctccggc ctctgcggtg ttggggaagt
     2941 cttccgcagg tgaggttgga gctcttcgac tatgtcgtcg ataccctcga ccagctttgc
     3001 accctccttg atgagtctgt gggtcccctt cgagccgggt gagtcgacgg cccccggtac
     3061 ggcaaagacc tcacggccct gctccagggc tgtcctggcc gtgatcaagg agccgctctt
     3121 gtcggttgcc tctacgacga cgactcctag agaaaggccg ctgatgatcc ggttcctcat
     3181 cgggaagttc gccgcgtggg gttgtgttcc gaaaggatat tcagtgacga cggcgccgtt
     3241 tgccgcgatt tcatcgtaga gttttttgtt ttccaatgga taatagacat cgattccgca
     3301 gccgagcacg gctatcgtcc gtccgttcgc ggagagggca ccgcggtgtg cggctgcgtc
     3361 gatcccgcgg gccatcccac tcacgatagt gaatccgtat tgcgcaagct cacggcatag
     3421 tctttcggtc aaaaatttac cgtaggtcgt ggccgccctt gagcctacga cggcgatcgt
     3481 cgcctcatct tccacgaggt tcccttttac gtagagtaga ggcgggcggt cgtagatgtc
     3541 gagaagtagc cgggggtaag aggcatcctc tacggtaacg agagaaaccc ctttttcccg
     3601 ggccaaggca agctcgcgtt cgacctgctc ccacctgttg aagcgatgga tgattttggc
     3661 cgtcttttct tttatgccgg gcacgctttt cagggcttcg agcggcgctt ctaagaccct
     3721 ccggggcgtc ccgaaggcgg aaagaaggtt cttgaccccg acactcccga gtccttcgac
     3781 gagcttcagg gccagccagc atttaaggtt gttctcatcc atcggggtaa ctacaattat
     3841 tttccttgac tttccatgac ctgtagaatg atttcaccag gcgggggccg aaaatatagc
     3901 ttattattat gacctgtcaa gctgaatctg ttcgagcgtg gcttttcatg ttgacagact
     3961 tgaaggcctt gacttacact cccagtgaaa tccttgacgt atctcgcagt ttgggggagg
     4021 tatgaaaatc gaacttgtcc atccaatcgg tgtgttcgac tccggaatcg gcgggatgac
     4081 cgtcgtgcgc gccctcatgg aaaggctgcc cttcgagaac atcatatact tcggggacac
     4141 ggcccgcgtg ccttacggca tcaggtccgt ggagacgatc accgagtacg ccacacagat
     4201 tgcaaatttc ctccttaaac agaacgtaaa acttctcatc attgcctgca acaccatcgc
     4261 ctcggtcgcc aacgaacgta tacaggaaat gtcccccgtc ccggtcctgg acgtcatcga
     4321 cgcaggggcc cgcatcgccg tggcggagac gaggaacaag atcgtcggcg tcatcgggac
     4381 gccggcgacg atcaacagca acgcctatgc acgggcgata aaccggtatg acccagaaat
     4441 ccgtgttttt tcccaggcct gcccgctctt cgtgcccctc gtcgaggagg ggtggttgga
     4501 ccacccggtg acgaggctca ccgcgcagga gtacctgaag ccggtcatcg ccgagcgtat
     4561 agatacccta gtcctcggat gcacgcacta cccgctgctc aaacctctcc tacaggaaat
     4621 cgtcggtgag ggcatccgtc tggtcgattc cgcggaggcc atggcggccg tcacggccaa
     4681 tctcctctcg gaaaagggcc ttgctaaccc cgtacgcctt cccccacaat ataggttcta
     4741 cgtgacggac gttccctacc gcttccagac ggtcggtgaa cgcttcctgg gaaggaccct
     4801 tgccaacgtg gaggtcgtaa aattacagtg aactcgggga cgcatgaagc gttcctgagg
     4861 tcgtaccgaa ggtgtctttt tctcttgccg cagagtccgc tttcccaccc gtcattttct
     4921 ttcgccctcg tgctgtttag gattgagacg gccctccttg gaaaagggga aggtcagact
     4981 tccgaagatg gcctcgattt ctgcccttcc ctctatcttg taaggaaggt cgctttccat
     5041 gacgagggcc ttcaggctgc cgccgaggtt cttgaagttt gcggcgacgg gtgcctgcac
     5101 ggcggtcgtc gacgacgcag gaaccaggac ctcgctctcg aggcgtccac tcccgatttc
     5161 ctcgttgttg agatagaccc tatactcgaa ggatttgatg gtgaggtcaa aacggtttgg
     5221 gttctgtacg tcgaggccga tcaggaggtt catctccagg agactgcggg ggcttatcgt
     5281 cacctcccgg aggatgaagg tcggcttttc cacaacccgg ttaagacaag aagcgaaggc
     5341 aacaaacagt acaatcagga agagcaatcc tgtacgcacc gtcacttttt catactgccg
     5401 tttctcttcc aaggaatcac atcctttctc tttcaaacag ctgcatgacg gcctccgcca
     5461 taagcttgta tcccgccgtc gtggggtgga cgaggtcggc ctggaccagg gttcggaact
     5521 cgacgtcctc gccgatcctc tttttccagt gagaatgaac gcgggccacc ggcagaccgt
     5581 tgaccgtcgc gaggttttcg agctcatcgt agaaatcctc cacgaagcgg gtctcgcggt
     5641 tgttcccgac gtaacttgag gtgacgagca cgatctcggc atcgccgtct tccctgatct
     5701 catcgaccat ccgctgtata tactccccga atcgttcggc gctgtaaccg cagaaggcgt
     5761 cgttgagggc gaactggatg aagacgcagt ccgggttatg gtcgaggacg tccgctctaa
     5821 gtcggtaaag ccctccttcc gccgtgtcgc cggggatgcc gctgttgacg atgtgaaagc
     5881 ggctttccgg gtatgcctgg cggagcattt ccgcgagaaa gtcgagaaac ccttttctca
     5941 ccatccaccc ttgcgtgagt gagtccccga tggcgacgat ggtggtctcg gctcctgatt
     6001 tcagcttctc gatcgtccga acaggtttca tgagcgattc tcttagatta tttcgcaacg
     6061 gcggtcaagg taaaagtatt gtaactattc aggctttacg ctatagtgcc ggccgcgccc
     6121 cccatggggg tattgacgat taccgggctt gaaggcaggc gcctcggcgc cggtggggtg
     6181 aggttggttt ccgggatgcg gtcacgtttg cgattgaaga acatcgtcct gtccgttgcg
     6241 actttcgttt tcgccgcgat gctcctgaac atcggctact acctcgtctt tcccgacgta
     6301 tcgaagctca agaaagtgaa cccccggaag acgtcattca tggaatacag ggaaaaggag
     6361 tggcaggccc agggaaagaa gaggaaaatc cggtacgtct ggacacccct ttcgcagatt
     6421 tcaccctacg tcgtcaaggc cgtgttgatc gccgaggacg ataagttctg gcgccatgaa
     6481 ggtttcgatt tcgaggccat gaaaaaggcg ctggaaaaag acatcaggaa gggaaagttc
     6541 agggccggcg gcagcacgat cagccagcag ctcgccaaaa acctgtactt gacaccctcc
     6601 cggaaccctg tccggaaggt caaagaggcc atccttacct ggaggatcga gcggaacctc
     6661 acgaagagaa ggatcatcga gctctacctg aacgttgccg agtggggcga ggggatattc
     6721 ggcatcggag tcgctgcttg gcattattac gggaaaccgg cgtcggcgct cggtcccatg
     6781 gaggcggcgc gacttgccgc ggtactgccg aacccgaggc attggagtcc tacgggggct
     6841 tcttcttatg tggagagccg gtctcagcga atctaccaga tcatggtacg caggggtatc
     6901 gtcatcgaag attacgacga ggtgatgaac acaccccagg aggagacggt ggacgaaaag
     6961 ccggaagcga cgacgacccg ggaaagtccc gagggaaagg agacgcccgg gactgaagga
     7021 acctctttat cttcaccgag gaaggatgaa atggaagaac aggggggaga tgcaacggaa
     7081 gaatgagaaa gggtaggaag gcaatgttat ttttctatcg aaatcgaacg ccggaggctt
     7141 ctttatgtct gcgatctttc ttttcctgac aggcctttcc cttttcctct acggcatggt
     7201 ggagctcagc gccctgatgc gtgaacgctt caacaacgcc cgcctccggg aaaatttcaa
     7261 gtacgcggtc cgcaagcctg tttacgggct tgttacgggg gtcgtcgcta cggtcctttt
     7321 tcagagcagc acggcaacgt cgctcctcgt cgtggggatg gtgagcgccg gcctgatgag
     7381 tttttaccgt tccctgagca tcctgctggg cgcggatatc gggacggtct ttacggtcca
     7441 gctcgtcgtc tggaaggtca cggacatctc tccccttttg gtcatcgccg gtggcctttt
     7501 atgggcgttg gggaaggacc gttggaaacc cctcggggaa ggggtgttct attttgggct
     7561 gatgttcttc gggttgagca tcgtttccgg ggtgaccgcg cccttgaagg acaacccgga
     7621 tgtcgtccgt ctgctccgtg agacaggcgg ccccctgaca ggcctgctgg cgggggtatt
     7681 gatcgccgcc ctggttcatt cctcggccat tcccatcagc atcctcgtta tcctggcgca
     7741 gtatcagatg gttactaccg agagcgccct tcccatcgtc tttggggcca acgtgggaac
     7801 ggccgtgacg gcgatcatgg cgagtatagt gaccaacgtg gacggcagga gaagcgccct
     7861 ttcccacctt ctcttcaagg tatttgggac agccgtctgt ttctcggtca tccctgtctt
     7921 cgttgggttc ttgaagctat tgtccgccga tacggcccag cagatcgctt acggacatct
     7981 ccttttcaac ctggtgaccg cgggctgttt catctttctg ctcaggccgt tttcccgcct
     8041 cgtcgaatac ctcctcccgg ggaaagcgga tacgctgccg gtctggccgg agttcctcga
     8101 cgcgcgctcc ttggctgtac cggaggaagc cctcgagtgc gtcaggaagg aattgcacag
     8161 agagatgact ctggcccgga gcatgttctt gaactccgtc cggttgatcg atgactatgg
     8221 cgagagaagg cgaaaagaca tcatgtatgt ggagctcgtg gtcgacaacc tgagaaatga
     8281 ggtctccgag tacctgcgta aggtatcagc cggagagctt tccgcggctt tgtcgaaaag
     8341 gcttttcagc tatgcggcga tggtcgacga catcgagaga atcgccgacc attcggtgaa
     8401 catcgtcgag ttcagtcgct gcaagcatct gaggaatgcg aaattttctc cgatggcgga
     8461 ggcggacctt gcagagatca tccgtctcat cgatgaaaat ctgagggcga gcctatctct
     8521 tttggaagag cgggatgaag acaagataag ggacatcagc ctgcgggagg acgaagtgga
     8581 ccgaaaggtc aaagaggcga gagagagaca tctggagcga ttccacaaga ggatctgtca
     8641 ggcggaggca gggcccatct tcgtcgagtt actggtcaat ctcgaacgga tatcggacca
     8701 ttgccagaac atcgccgatt acgtggcgga actcgaaggt actccgtctc ttccggcggc
     8761 ttgagtccgg cgaggcccgt ttttacggtc aaagcgccgc tttgaccggc ctgcgcggat
     8821 ggttgtagag agaaaatcgc ggagctgggg gccgagggtg cccgcagccg attctctgct
     8881 ggaaaagttc gcggaaatag agtaagaaac ctgcccacgg taagatcagg aaaaagtcaa
     8941 tatggatgta aggagcggaa atcatgggaa ataataccaa aagcccttat ttttcggggg
     9001 cttcgagata tgtgctcgac gacgagcttg caaggatcgt gaatatttcg atggcgctgg
     9061 agatgccgct tcttctcaag ggtgagccgg ggacgggtaa gacaatgctc gcccatgcca
     9121 tcgccgagag cctcgacatg cccctcatcg tcctgaacgt caagtcgagc atgaaactcg
     9181 tagatgccct ctatcagtac gataccctca cccgtctgaa cgacagtcgc ttcggcgatt
     9241 cgaagaggga cgtgagcaac atagaggatt atatccggat gggtaagata ggccaggcct
     9301 tcgtctccga ggtgcggacg gtcctcctca tcgacgagat cgacaaggcg gacaccgact
     9361 ttcaggacga tatgctggac gtactggacc agatgcagtt cgacatcatt gaggtcgata
     9421 agaccgtctc cgccaaacac cggccggtga taattatcac gtcgaacgcc aagaaggacc
     9481 tct
//
LOCUS       DAOTMX010000023         9228 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_6862_length_9228_cov_4.813692, whole genome shotgun sequence.
ACCESSION   DAOTMX010000023 DAOTMX010000000
VERSION     DAOTMX010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 9228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9228
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6862_length_9228_cov_4.813692"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..1188)
                     /locus_tag="P5573_01285"
     CDS             complement(28..1188)
                     /locus_tag="P5573_01285"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRT26731.1"
                     /translation="MKRTVCLFTLLATFIIALPQGFAVPQPPGKGPFDLPDLIEYAVR
                     HNPALKIAQQNVEIENQGLKAAQADRMPRIDVTGGYTRYRYPAPLTPIVITGLPLAST
                     DFPDFERNVYDGFALLSVPLFKGGRLMRGVTIAELKKSMARDFHTRNLHELVYNVTAV
                     YYKLAQLHELLRASEAQIKGLESHRRDVEYSLKAGTAPRLDLLKADTELGAAMERRIQ
                     TKNNIANVKELLRSLIGMDDPSELLIIFNDGPPRDSVALNADLEGVLAARPDHRAALS
                     KVKMLEERVASARGKRLPDIYGTGDYGGKAGDGLAFKENWSVGVRLLLPVFDFGRIGA
                     EIDREQLELTKAKEEERALRLAIARELKEAQTAILNADERIAVSGRLDPTGS"
     gene            complement(1185..1832)
                     /locus_tag="P5573_01290"
     CDS             complement(1185..1832)
                     /locus_tag="P5573_01290"
                     /inference="COORDINATES: protein motif:HMM:NF020771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CerR family C-terminal domain-containing
                     protein"
                     /protein_id="HRT26732.1"
                     /translation="MTDISLDTRSRILDAAGEIFAVSGFRQATVRQISARAGVNVAAI
                     NYHFQSKDNLYLETMRHWKNIAFRKYPGELGTSQTDPPEKRLEGFIRSFVFRILDTGV
                     ESRFGRLMAREFAEPTVALDVIVEETARPMLGLISSIVGRITGQPPESNTVLYCCASI
                     VGQCLYFLYARPVLLRLVGQDRVRSMEMDDIARHIFRFSLAALYSIRDEKTGDPQ"
     gene            2288..2803
                     /locus_tag="P5573_01295"
     CDS             2288..2803
                     /locus_tag="P5573_01295"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRT26733.1"
                     /translation="MKKESGQNPALCYQCGNCTAGCPYTQYFDYPVSMVMRLLQAGQK
                     ETILTSKAIWLCATCETCTTRCPCEVDVAHIMDTLRIIARREGKVSEKDVQLFYDSFL
                     DSMKKHGRIFEMGILMGYKFKSGQFMGDAELGPKVMSKGKISLFPKTIKGADKVAQIF
                     NRFQEKAKKHG"
     gene            2796..3764
                     /locus_tag="P5573_01300"
     CDS             2796..3764
                     /locus_tag="P5573_01300"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HRT26734.1"
                     /translation="MAEKEYAYFSGCSLEGTAKEYDESLRAIMKALGVNLVEPDDWSC
                     CGSTPAHTVDHVFAAALAARNLTLVEKMNKDVLTTPCPSCLMAFKKAQFNMSRDEAFK
                     DEVNGLLDEAYNCGVESKSSLQVIYEDIGLDAIAARVTHIMPDLKVAPYYGCILSRPP
                     EIAQFDDPENPVSMDKVLSAAGIQVQDFAFKMECCGAAFGVPKREMVNRLSGKVLEMA
                     IDAGANCIAVACPLCQQNLDLRQEQVNKTTGSNYNIPVIYFTQLIGLAYGLSPKELGM
                     DKVIVSADSITRRITREEYEKIKADEAAAKKPQKAKKDAAAEETNN"
     gene            3778..5739
                     /locus_tag="P5573_01305"
     CDS             3778..5739
                     /locus_tag="P5573_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HRT26735.1"
                     /translation="MRVGVFICHCGSNIAGTVDVAKVAEEARKMPGVAYATAYMYTCS
                     EPGQKEITDAIERDKLDRIVVAACSPRMHENTFRRTIMKAGLNRYFFEQANIREHVSW
                     IGLDKELNTQKAIEEVRMAVAKVMKNKPLFSSSFKINKRVLVIGGGVAGMQAALDCAD
                     GGLEVVMVEKSPSIGGMMARLDKTFPTVDCSICILGPKMVDVAQHDLIKLHAYSEIAE
                     IKGYVGNYQIKIKKKPPYVDWTKCTGCGLCMEKCPTKNAYDHFNFGAAPTRAINIPFP
                     QAIPKKATIDPNHCRQFVKGKCGVCAKVCPTGAINYEMQDEFITEEVGAIITATGYGL
                     MDIERLTEYGGGRYPDVITGIQYERFLNASGPTSGHIIRPSDHEEPKTIVFVACAGSR
                     DKSLGVPYCSNFCCMYIAKQAILTKDHIPDSQSYVFYMDVRSPGKGYDEFTRRAQEEY
                     GAKYIRGRVSRIYPKGKKMVVKGADTLLGTQVEIEADLVVLGTAVVSAPGAAQMAEKL
                     HISYDTFGFYVESHPKLRPVETNTSGVFLAGACQGPKDIPASVGQGSAAAAKVLSLFS
                     KDMLESDPAIAQVNQNTCVGCLKCLKTCPFQAIVEDILRSGKKVAKVIETVCAGCGVC
                     TSTCPCGAIQLQHFTDNQLLAEVNAICQK"
     gene            5727..6143
                     /locus_tag="P5573_01310"
     CDS             5727..6143
                     /locus_tag="P5573_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015904076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HRT26736.1"
                     /translation="MSEVAAKELRIVGFLCNWCSYGGADTAGVGRFKQPTDLRIIRVP
                     CSGRVDPMFVVRALITGADGVLVSGCHPRDCHYTDGNFYARRRLEMLKRLLPFLGIDD
                     KRFHYTWVSASEGAKWQQVVTDFTKQVHALGPMHQK"
     gene            6156..7106
                     /locus_tag="P5573_01315"
     CDS             6156..7106
                     /locus_tag="P5573_01315"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRT26737.1"
                     /translation="MSTQKLKEIIKQVLPDVETVICWQDGFDKLNVTPIFITSPEQAD
                     KVVWNPLCAQNLASYLPSQKKKVAVAVKGCDSRTIVQYMQEGLIDRDNVVIIGIPCKG
                     VVSKKKVQKAIGNEPVEDVTFTDGSVKVKTPSGEKTFAIADVCPGKCASCQYPTPIVY
                     DHLTSEAIKSDKPAESVYADVAELEGKTLEERKAYFLGQFDKCIRCYACRNACPMCVC
                     QDSCIAETREPHWITQKLSLDEKLMFHMIHSLHLAGRCVECGECERVCPMEIPVAKLK
                     KKINKEMRDLFDYTPGVNPEDKPPMYTFNVDEAKIKEHKL"
     gene            7106..8191
                     /locus_tag="P5573_01320"
     CDS             7106..8191
                     /locus_tag="P5573_01320"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRT26738.1"
                     /translation="MAEKGYLPKEKIKEFVEALGKDATVFAPCLEGDTIVFREYAAGK
                     EISLDRPANTPPKGAIYPQCETLLSFEFKKEADSPQKMDVELKVNTDFPKAVVFGGRP
                     CDARGFTVIDRVFVNTDTADPYYKKRRENTTIVTKCCNGPVAGCFCVGVGGGPAVTEG
                     SDVVMTEVDGGYYLEAVTDKGKAVMSLPMIQDGASYEAKAKQANEKATAAVRNPFPAG
                     ADKISGDLFDSGDFWGRVIDKCLSCGACTFLCPTCYCFNITDEQAITTGERIRSWDSC
                     MFQHFTLETSGHNPRPVKHKRFRNRVGHKFYYYPEKYDGAIACTGCGRCIRYCPVSVD
                     ISEIVGYLRDPKADPKEWAAEKKDAKN"
     gene            8201..9037
                     /locus_tag="P5573_01325"
     CDS             8201..9037
                     /locus_tag="P5573_01325"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HRT26739.1"
                     /translation="MTGNENPYLPELATVTKVIEETPNIKSFQVVFNDAEKMKNFKFE
                     PGQVGQLSVFGVGESTFVINSPPTRMEYLQFSVMKAGEVTTALHGIYEGDVIGVRAPL
                     GNWFPYNDMKGKNILFIGGGIGLAPLRTLILYMLDNRADYKDITIIYGSRTPPDLCYK
                     DELKEWEERSDVNLILTVDNEFPGWEKRTGFVPTVLKEEAPKADNTIAITCGPPIMIK
                     FVLEGLAQLQFPEENIITTLEARMKCGIGLCGRCNLGHKYICKDGPVFSLKQLKELPS
                     DE"
BASE COUNT         2182 a         2444 c         2684 g         1918 t
ORIGIN      
        1 gccaaagggg cagcaaaaga caggatttta gcttcccgta ggatcgagac gacctgatac
       61 ggcgatgcgt tcgtcggcgt tgaggatggc ggtctgggcc tccttgagct ccctcgctat
      121 ggcaaggcgc agcgccctct cttcctcctt cgccttcgtg agttccagct gttcgcgatc
      181 tatttcggcc ccgatacgtc cgaaatcgaa gacgggcaga agaaggcgaa cgccgacgct
      241 ccaattttcc ttaaaggcca gaccgtcgcc cgccttgccg ccgtaatcgc cggttccgta
      301 tatgtcgggg agccgcttgc cccgtgccga cgcgacgcgc tcctcgagca tcttcacctt
      361 gctcagtgcg gccctgtggt cgggccgggc ggcgaggacg ccttcaagat cagcattcag
      421 ggcgacgctg tcgcggggcg gcccgtcgtt gaagatgatg agcagttcgg agggatcgtc
      481 catgcctatc agagacctga gaagctcttt cacgttggcg atgttgttct ttgtctgtat
      541 ccttctttcc atggcggctc ccaattccgt atcggccttg agaagatcaa gcctcggcgc
      601 cgtgccggcc ttgagagagt attcgacgtc ccggcggtgg gattccaggc ctttgatctg
      661 cgcctcgctg gcgcgaagca gttcatggag ttgagcaagc ttgtaataga cggcggtaac
      721 attgtacacg agctcgtgga gattacgtgt gtggaagtcg cgggccatcg acttcttgag
      781 ctcggcgatg gtgacgcctc tcataaggcg tccacccttg aacaggggga cggaaagaag
      841 ggcgaagccg tcatagacgt tcctctcaaa atcgggaaag tcggtggagg caaggggcag
      901 gcccgtgatg acgatcggtg tgagcggcgc cgggtaccgg tagcgggtat agcctcccgt
      961 cacgtcgatc cgcggcatcc tgtccgcctg ggctgccttg agaccctggt tctctatttc
     1021 aacgttctgt tgcgctatct tgagagcggg gttgtgtctt acggcgtatt ctatgaggtc
     1081 cggaaggtcg aaggggccct ttcctggagg ctgcgggacc gcgaagcctt gaggaagggc
     1141 gatgatgaat gtagcgagca gggtgaaaag acatactgtc cgtttcattg tggatctccc
     1201 gttttttcgt cccggatgct gtaaagcgct gcaagggaaa agcgaaagat gtgccgtgct
     1261 atgtcatcca tctccatgga acgtacccgg tcctggccca ccagtctcag gaggaccggc
     1321 ctggcataga gaaagtagag gcactggccg acgatactgg cgcagcaata gagaacggta
     1381 ttgctttcag ggggttgccc ggtgatgcgg cccacaatgg acgagatgag accaagcatg
     1441 ggtcgggccg tctcctcgac gatgacgtcg agggcaacgg tgggttccgc gaattccctt
     1501 gccatgagcc tgccgaaccg tgattcgacg cctgtgtcca ggatacggaa cacaaaggag
     1561 cgtatgaagc cttcgagcct tttttcgggt gggtcggtct gggatgttcc cagttccccg
     1621 gggtatttcc ggaaggcgat gttcttccag tggcgcatgg tttcgagata gaggttgtcc
     1681 ttgctctgga agtgataatt gatggcagcg acattgacgc ccgcccgcgc gctgatctgc
     1741 cggaccgtgg cctgcctgaa acccgatacc gcgaagatct ctcctgcggc gtcgaggatc
     1801 cggctcctcg tatccaaact gatatctgtc atattcgtcc catgttaaac aaacgtttca
     1861 tacagatatt ttaaacgaca gttttatatt gtcaagaaat ttttttcaca tgattccttg
     1921 tgaagagaat tacatagtgt tggaaaaaag accctgtgtt atcaacagga tgagaagggt
     1981 taacaagaga ggtattcatt atcctgaatc gttgccgggt caaatatgta ttgacaagga
     2041 aaatcgaagt agtgttaaat accacagggt tttaaggggt atagttcatt gataagcagt
     2101 tcagttctga tagttcactc atagtgttca ctatagtgaa tatatttccc ggagcagtca
     2161 ttgacgagtt cacaggcaca atctaactct gacaaacggg ctcactgctg ccggctctaa
     2221 aggttggtta atctgaatgg atacgatcaa tttaactgaa aaaaccgatt gggctttcat
     2281 tgaagaggtg aaaaaggaga gcggtcagaa ccctgcgctg tgctatcagt gcggcaactg
     2341 caccgcgggg tgtccataca cccagtactt cgactacccg gtcagtatgg tcatgcgcct
     2401 tcttcaggcc ggacagaaag agacgatcct tacctccaag gcgatatggc tctgcgccac
     2461 ctgcgagaca tgcaccacac ggtgtccctg tgaggtcgat gtggcgcata tcatggatac
     2521 gctgaggatc atcgcccgcc gcgagggcaa ggtatccgag aaggacgtcc agcttttcta
     2581 cgattcattc ctcgattcaa tgaagaaaca tggcaggatc ttcgaaatgg gcatcctcat
     2641 gggctacaag ttcaagtcag gccagttcat gggagatgcg gagctcgggc cgaaggtcat
     2701 gagcaagggc aagatcagct tgttccccaa gacgataaaa ggtgccgaca aagtagctca
     2761 gattttcaat agattccagg aaaaggcgaa aaagcatggc tgagaaagaa tacgcatatt
     2821 tcagtggttg ttcgctggaa ggtaccgcga aggagtacga tgaatccctg cgggccatca
     2881 tgaaggccct gggggtgaac cttgttgagc ccgacgactg gagctgctgc ggctccacgc
     2941 cggcccacac ggtcgatcac gttttcgccg cggcccttgc tgcacggaac ctcaccctcg
     3001 tggagaagat gaacaaggat gtcctgacga caccctgtcc ttcgtgcctc atggcgttca
     3061 agaaggcaca gttcaacatg tccagggacg aggccttcaa ggatgaggtc aacgggcttc
     3121 tcgatgaggc ctataactgc ggcgtcgaat cgaagtcctc tttgcaggtg atctatgagg
     3181 acatcgggct ggacgccatc gccgccaggg tcacccatat catgcctgac ctgaaggtag
     3241 cgccttacta tggatgcatc ctctccaggc cgccggagat cgcacagttc gacgaccccg
     3301 aaaacccggt ttcgatggac aaggtgcttt cggctgccgg tatccaggtt caggatttcg
     3361 cattcaagat ggaatgttgc ggggcagcct tcggcgtccc gaagcgcgaa atggtgaaca
     3421 ggctctccgg caaggttctc gagatggcca tcgacgccgg cgcgaactgc atagccgtcg
     3481 cgtgcccgtt gtgccagcag aaccttgatc tccgccagga gcaggtgaac aagacgacgg
     3541 gctccaacta caatatcccc gttatctatt tcacccagct tatcgggctt gcctatggat
     3601 tgtcacccaa ggaactgggt atggataagg tgatcgtaag cgccgacagc ataacccggc
     3661 ggatcaccag ggaagagtac gagaagatca aagcagacga ggcagccgcc aagaagcccc
     3721 agaaggccaa gaaagatgcg gctgctgaag agacaaataa ttaaggagac ctgatcgatg
     3781 cgggttggtg tatttatctg ccattgtgga agcaacatcg cgggaaccgt tgacgtcgcg
     3841 aaagtggccg aggaagcccg gaagatgccc ggcgtcgcct atgcgacggc ctacatgtat
     3901 acctgttcag agccgggaca gaaagagatc acggatgcca tcgagcgtga caagctcgac
     3961 aggatcgtcg tagcggcatg ctcgccgcgt atgcatgaga acaccttcag aagaacgatc
     4021 atgaaggcgg gactcaaccg ctatttcttc gaacaggcca acatccgtga acacgtgtcc
     4081 tggatcggcc tcgacaaaga gctcaacact cagaaggcga tcgaagaggt caggatggct
     4141 gtggcaaagg tcatgaagaa caagcctctt ttctcatctt ccttcaagat caacaagaga
     4201 gttctcgtga tcggcggcgg cgtggctggc atgcaggcgg cgcttgactg cgcggacggc
     4261 gggctcgagg tggtcatggt cgaaaagagc ccctccatcg gcggtatgat ggcgcgtctc
     4321 gacaagacct tccccaccgt cgactgttcc atctgtatcc ttggtcccaa gatggtcgac
     4381 gtggctcagc acgacctcat taaacttcac gcatacagcg agatagccga gataaagggc
     4441 tatgtcggca actaccagat aaagatcaaa aagaaaccgc cttacgtcga ctggacgaaa
     4501 tgcacaggtt gcgggctttg catggaaaaa tgccccacca agaatgctta cgaccatttc
     4561 aacttcggcg cggcgcctac gagggccatc aacataccct tcccgcaggc gatccccaag
     4621 aaggcgacga tagaccccaa ccattgccgg cagttcgtca agggcaagtg tggtgtctgc
     4681 gccaaggtct gccccacggg cgcgatcaac tacgaaatgc aggatgagtt cattacggaa
     4741 gaggtgggtg ccatcatcac ggcgacgggt tacggcctca tggatatcga aagacttacc
     4801 gagtacggtg gcggacggta tcccgacgtc atcaccggta tccaatacga acggttcctc
     4861 aacgcctcag gccccacttc gggccacatc atcaggcctt cggaccacga agagccgaag
     4921 accatcgtct tcgtcgcctg cgccggttcc cgggacaaat ccctgggtgt tccctactgt
     4981 tcgaacttct gctgcatgta cattgccaag caggccattc tgacaaaaga ccacatcccc
     5041 gattcccagt cctacgtctt ttacatggac gtacggtccc cgggcaaggg ttacgatgag
     5101 tttacgcgcc gagcgcagga agaatacggc gccaagtata tccgcggcag ggtatcacgg
     5161 atctatccga agggaaagaa gatggtggtg aagggagccg atacgctcct tggcacccag
     5221 gttgagatcg aggctgacct cgtggtcctc gggacagcgg ttgtttccgc accgggtgcg
     5281 gcccagatgg cggagaagct ccacatctcc tacgacacct ttggattcta cgtggagtct
     5341 cacccgaaac tgcggcccgt cgagacgaac acgtcaggtg ttttcctcgc cggcgcctgc
     5401 cagggcccca aggacattcc ggcatccgtc ggtcagggat cggcagccgc cgccaaggtc
     5461 ctgtcgctct tctcgaaaga tatgctcgaa tccgacccgg cgattgctca ggtcaaccag
     5521 aatacatgcg tgggctgtct aaagtgcctt aagacctgtc ccttccaggc gatcgtcgaa
     5581 gatatcctga ggagcggcaa gaaagtagct aaggtcatcg agacggtctg cgccggatgc
     5641 ggcgtctgta catccacctg tccctgcggc gcaatccagc tgcagcattt cacggacaac
     5701 cagctcttag cggaggttaa tgcaatatgt cagaagtagc agccaaagaa ctgcggatcg
     5761 ttggttttct ctgtaactgg tgttcctacg gcggtgccga taccgccggc gtgggtaggt
     5821 tcaaacagcc caccgacctt cggatcatca gggttccctg ctcgggccgt gtcgacccca
     5881 tgtttgtggt ccgtgctctc atcacgggtg ccgatggtgt tcttgtttcg ggatgccacc
     5941 ccagggattg ccactacacg gatggcaact tctacgcacg gcgcaggttg gaaatgctca
     6001 aaaggcttct gccgttcctg ggcatcgatg acaagagatt ccactacaca tgggtttcgg
     6061 cctctgaagg cgcgaagtgg cagcaggtcg taacggactt cacgaaacag gtccacgcgc
     6121 tcggccccat gcatcaaaag taaggagaag gcagattgag tactcagaag cttaaagaga
     6181 tcatcaagca ggtgctcccc gatgtggaga ccgtcatatg ctggcaggac ggttttgaca
     6241 agctcaatgt gacgccgatc tttataacgt cccctgagca ggccgacaag gtcgtctgga
     6301 accccctgtg tgcacagaac ctcgcaagct acctcccatc gcagaagaaa aaagtggctg
     6361 ttgcggtgaa gggctgtgat tcgaggacga tcgtgcaata tatgcaggaa ggcctcattg
     6421 acagggacaa cgtggtcatc atcggtatcc cctgtaaggg agtcgtcagc aagaagaagg
     6481 tacagaaggc gatcgggaac gagccggtgg aggacgtgac attcacggac ggttcggtaa
     6541 aggtcaagac gccgtcgggc gagaagacgt tcgcgatcgc cgatgtctgt cccggcaaat
     6601 gcgcctcctg tcagtacccg actcccatcg tctatgatca cctgacgtcg gaggccatca
     6661 agtcggacaa gccggcggaa agtgtctatg ccgacgtggc ggagctggaa ggcaagacgc
     6721 tggaagagcg caaggcgtac ttcctgggcc agttcgacaa gtgtatccgc tgctatgcct
     6781 gccggaacgc atgccccatg tgcgtgtgtc aggacagctg catagcggag acacgcgaac
     6841 cgcactggat aacccagaag ctgagtctcg acgagaaact gatgttccac atgatccact
     6901 ccctccatct cgcgggacgc tgtgtggaat gcggtgaatg cgaacgggta tgtccgatgg
     6961 agatccctgt agcgaagctg aagaagaaga taaacaagga gatgagagat ctcttcgatt
     7021 ataccccggg agtcaacccc gaggacaagc cgccgatgta cacctttaat gttgacgaag
     7081 ctaagatcaa ggagcataaa ctctaatggc cgaaaaagga taccttccga aagagaagat
     7141 aaaggaattc gtagaggccc tcggtaaaga cgcaaccgtc tttgctccat gcctcgaagg
     7201 ggacacgatc gtcttccgtg agtatgctgc tggcaaggag atctccctcg accggccggc
     7261 gaacacgccg cctaaagggg ccatctatcc ccagtgcgag acgctgcttt ccttcgagtt
     7321 caagaaggaa gcagacagtc cccagaagat ggacgttgag ctcaaggtga acacggactt
     7381 cccgaaggcg gtggttttcg gtggacgtcc ctgtgatgcc agaggtttca cggtcatcga
     7441 cagggtcttc gtcaataccg acaccgcgga tccctattac aagaaacgcc gtgagaacac
     7501 gacgattgtc acgaagtgct gcaatggccc ggtggccggt tgcttctgtg tcggcgtcgg
     7561 aggcggtccc gccgtgaccg aggggtcgga cgtggtcatg accgaggtcg atggcggata
     7621 ctatcttgag gccgtcacgg acaagggcaa ggccgtcatg tccctgccga tgatccagga
     7681 cggggcgtct tacgaggcga aggcgaaaca ggccaacgaa aaagctaccg ccgcggtgag
     7741 gaatcccttc ccggcaggtg cggataagat atcgggcgat ctgttcgaca gtggagactt
     7801 ctggggccgt gtcatcgaca agtgcctgag ttgcggtgcc tgtacattcc tgtgtccgac
     7861 ctgctactgc ttcaacatca ccgacgagca ggcaattacc acgggagagc gcataaggtc
     7921 ttgggattcg tgcatgttcc agcatttcac tcttgagacg agcggtcaca acccgaggcc
     7981 ggtgaaacac aaaagattca ggaaccgcgt cgggcacaaa ttctactact acccggaaaa
     8041 gtacgatggc gccatagcat gtacgggctg tgggagatgc atcaggtact gcccggtatc
     8101 cgtggatatc agcgagatcg tggggtacct gagagacccg aaagcggatc cgaaggaatg
     8161 ggccgccgaa aaaaaagacg caaagaatta ggagtgcgtg atgacaggga atgagaatcc
     8221 ataccttcct gaattagcga cagttacgaa ggtaatagaa gagacgccaa acatcaagtc
     8281 cttccaggtg gtgttcaacg atgccgagaa gatgaagaac ttcaagttcg agccgggtca
     8341 ggtcggacag ctctcggtct tcggtgtggg cgaatccacc tttgttatca attccccgcc
     8401 gacgcgtatg gagtatctcc agttcagcgt catgaaggcc ggagaggtga caaccgctct
     8461 gcacgggata tatgaaggag atgtaatcgg cgtaagggcg ccgctgggca actggtttcc
     8521 ctacaatgat atgaagggga agaacatcct ttttatcggc ggcggtatcg gtctggctcc
     8581 gctgaggacc cttatccttt acatgctcga taacagagcg gattacaaag atataaccat
     8641 tatctatgga tcgaggacgc cccctgatct gtgctacaag gacgaattga aggaatggga
     8701 agaacgatct gacgtgaacc tcatcctcac cgtagataat gaatttcccg gatgggagaa
     8761 gagaacgggt ttcgtgccca cggtactcaa ggaagaagca ccgaaggccg acaacacgat
     8821 agccatcacc tgcgggcctc cgataatgat aaagttcgtt ctcgaagggc tcgcgcagct
     8881 ccagttcccc gaggaaaaca tcatcaccac gctggaagcg agaatgaaat gcggtatcgg
     8941 cctgtgcggc cggtgcaacc tgggtcacaa gtatatctgc aaggacggcc cggtgttttc
     9001 gctgaagcag ctgaaggagc tccccagcga cgagtaaaga acagtcagaa gttccacgtt
     9061 tcaggtttca agtttgaaac gcaagcagcc cccctatcgg gggttgcttt ttttatgctg
     9121 tggggtgcac cagtcagtaa ttgtgttgac agcccaccct ctttcacggt tttttcttgg
     9181 cgacctgcga cctgcgacct gcgacctgcg acctgcgacc tgcgacct
//
LOCUS       DAOTMX010000024         9226 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_6864_length_9226_cov_3.613346, whole genome shotgun sequence.
ACCESSION   DAOTMX010000024 DAOTMX010000000
VERSION     DAOTMX010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 9226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9226
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6864_length_9226_cov_3.613346"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1051
                     /locus_tag="P5573_01330"
     CDS             <1..1051
                     /locus_tag="P5573_01330"
                     /inference="COORDINATES: protein motif:HMM:NF013717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HRT26740.1"
                     /translation="DLIPDKRKEMLGAHYGVIPRLMTAAPSFFRRAVMEGKPYQMRGY
                     YGMCTNPMVAWADSRATYETFKSLDFSAVAEIFMTPTAAMADIVLPVAHQYEMNDIGH
                     YGIGHGIVLARPKVVEPPEECWPDMKILNEIGKRVSPPELWHEDHEEFLEDLLRPAGL
                     TYREFVEKGYLKGPDSFRTYETKGFPTPTGKVELRLSTAEKFKLKALPEFTGFPEEDD
                     PDYPLLVTSAKSRYYLLSSYRWVARLREKRPHPLVEIHPETAARYGIADGDEVVIETK
                     YGAITQRARVTDTIHSRVVSAALGWWFPEAAPETQYDWRRSNFNMLTSMKKLGKEFGT
                     PNITNLPCRIRRASA"
     gene            1148..2011
                     /locus_tag="P5573_01335"
     CDS             1148..2011
                     /locus_tag="P5573_01335"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRT26741.1"
                     /translation="MKVDHRRKIIDVFSDIDSHRVTAEIIRRHSTNRRDVREVALEGL
                     DLSRVRKIVELGCGFGFFTEALRARVSPGARIIGIDVVEGYRDVFMEACARAGIDGTF
                     IHGDASVIRDFEDRSFDAVICSYSLYFFPHIIGEVARIVDEDAVLVATVHNSDSLKEL
                     VDFTRDMLVENGMIDRAATFPIEALITGFSSENGYELLEPWFGAIIRKAYENTLVFEA
                     GDMYSLMEYFRFKWPLFLSGLSPRDVARAFDLFEVYLQRHFNRSRDGFRITKDDTIFV
                     CSKPRCRRDER"
     gene            2008..3024
                     /locus_tag="P5573_01340"
     CDS             2008..3024
                     /locus_tag="P5573_01340"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRT26742.1"
                     /translation="MKRAERRYCVHCGESVAKRDEDGVLRDYCAACDVFFYDNPLPVV
                     STILQEDRRILLVKRKYRPYKGTWCLPSGFAEVGEGIEDAAIRELEEETGIKGKIVSL
                     VDVDSCSNYFYGDLIFLTFEALRVAGKITPGSDTVAVKYYPLDKIPRLAFRSNMKAVR
                     TFIQNKSDYWAIIDSFSAAVEKRGGQRQKKNLLSDRLVETIRENADAIVANWLHDVSV
                     NRSTPNYHSYDPERLRRRAQDVLELFSTWADEFYKDSAQRSVDIRGYFRGFGREKRKE
                     GFTLHEALSALSLKRKHIWEFALSYGMTKKPLDIYMAFEFDRRMMIFFDRATFYSSMG
                     YMEV"
     gene            3118..4143
                     /locus_tag="P5573_01345"
     CDS             3118..4143
                     /locus_tag="P5573_01345"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HRT26743.1"
                     /translation="MFHEEKCDFCGECLERCYYLDFDRESGSKEFEKLVRGEKVNWLK
                     DCITCIACNEYCPKGARPFDLILRRLEETGDFTDPALRAQMAERFKPEGEPRPVEKKD
                     RVMSICVMQGNMPWAIQGQLFEGIPLLKGKHYFCNVLFAHMGDESIMRDRLRAMVDNL
                     ARSGAKEIVFVHDDCYGLLKGVAPEYGITLPFRPVHIFEYLRDYLKTHKDKVKKLDMK
                     VAYQRPCASRLTPEKDGLLDEIFDLIGVRRVKRQYDGIDALCCGVEVAGPGLKLFPRG
                     KNFAPFREKNVKDAKDSGAEAMVYLCPMCFATLNKPVREAGMKNYMISDICRLALGET
                     LPPEKPL"
     gene            4230..6032
                     /locus_tag="P5573_01350"
     CDS             4230..6032
                     /locus_tag="P5573_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT26744.1"
                     /translation="MKAANRKKESVEGISPDELLAGMGVTPADNAEGLTTAIPWRSLM
                     GGELTRGLFCWKRCLEAVGENPFLFGRLAMTAAGQKVVLESQVTEPIKKYARLAVVTK
                     KNPDVFGELNAWIYKSYAQMLYNFCGNFMTDNRFDRQKARYTVTTPDGKEFLKRTLAE
                     FAFLENNYNSRGLTRLHAMKGLLKCFLVLITGKPLQNGEMLFWRKKSGGTPSISFEEY
                     LEECRVRFENLYTNDAFDVRDYSDRATGGMIGYMPYSVVEGSSLHSVTLRHYPRPEGI
                     PPNGKVLYLASPLINKPEIYDLAKGKSVIEGMLRLGYDLYLVDHGEPGYRESKLGLDF
                     YGKTVHDRYLDILERLHPGSEIHTMGYCMGGTLLISYLARRAEERMALGLPMDIRKVA
                     LLATPVKWDDEESGMRPMRDVIRNYYDPYLMRELFGDVNVPPQLIEMGMNLTQPGVQF
                     YMASGLYERAHIPDDLEDSAPFLYWVTHGTKFPERAHEEWIAKMYMGNQIYEGTYCLP
                     SSVKALDGKPVNMNALKDAGVAIFNYRGDRDVIAPPGSCVAGDLWGVTEEGNISVSRG
                     GLNRTIEKHAGHIFVVSRTLLAEFLDLVSAFFKG"
     gene            6091..6969
                     /locus_tag="P5573_01355"
     CDS             6091..6969
                     /locus_tag="P5573_01355"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRT26745.1"
                     /translation="MVMPRDRFVTVNNVETRYWAVGDGKRPLVLIHGLGASAEIWMHN
                     IGAFSENHRVIVPDLPGFGRTDMPGEPFAPFDYLSFLDGFLRELKVERAVLCGQSLGG
                     GIALLYAIRFPEKVEKLVLVDCAGLGVEVIWTLRWMTLPLIGELSSVPTRPGVALFFK
                     LAVKNPAVVTDELIDLYHGYFNRPGRPAFLLRLVRSLVDFRRVKRDVVSPILENLHRV
                     ACPTLIVWGERDRVFPLSHGRHGLERIKGAEIRVMRDCGHIPFFERPGEFNELVLDFL
                     LPPLSCEGDQARPFDL"
     gene            complement(6987..8405)
                     /locus_tag="P5573_01360"
     CDS             complement(6987..8405)
                     /locus_tag="P5573_01360"
                     /inference="COORDINATES: protein motif:HMM:NF039904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRT26746.1"
                     /translation="MKRKKAEYASAYQGKALIADPIYQYAWFTVPSPDYPKESTEKDL
                     IDSPWVQRLRRINQLQSARWVYPAAEHSRFQHSLGTMHMAGEFGRYLYPSLKEVCGDV
                     PSLNFVEELLRVAGLLHDVGHGPYGHFFDDEFIRQFDLNHERIGQEIIKRKLAGIIRG
                     MRRSPTGPFEGRETIQPEQISFLIKMPEEGDRRHPQWLKMLRQLFSGVYTVDNLDYVQ
                     RDAYMTGFSMDIVDISRLRFYSFFTGKGLTLHQAGISALSRFLNARLNLYTNVYYHRT
                     TRALDLHLQEIFRETMDILFPHHPLEELDEYLKLDDWHLFEQVRAWPQARSAAKRRLG
                     KEWETIHSRAVKWKMAFAAELSIDEVRKGARFSTADDYEKEIRQFLAKDLKNLEFRVD
                     LATQDPRPLNPMAEEEKRINVFNPLTGLTSPEPLQDIYRFIPARVVHFRVFALNHDHD
                     RELSEAAEKALSTIGAGYRTNI"
     gene            complement(8408..9058)
                     /locus_tag="P5573_01365"
     CDS             complement(8408..9058)
                     /locus_tag="P5573_01365"
                     /inference="COORDINATES: protein motif:HMM:NF024791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HRT26747.1"
                     /translation="MGGKLQWMIIGIVTVFFFSGCASLKCLDGSCERQIQVLTITNDE
                     MKKELTRLEADVKEKDKALQSAKEEVAKLQSEKAALTAQVKDLQEYIEKQRQGEKAAA
                     IKTEAITEKEEVRKAEAASQGETLAKPLRIKVLSGDGRLASARKMAQLIEKTGLKVEI
                     VDHAPRSNFAEDTIYYTADAEKEAKEIAKSIGGKAVLKPVTWSSSFNIIAVTGKKK"
BASE COUNT         1948 a         2465 c         2740 g         2073 t
ORIGIN      
        1 cgacctcatc cccgacaagc gcaaggagat gctcggcgcc cactacggcg tcatcccgag
       61 actgatgacc gccgccccgt ccttcttccg ccgggccgtc atggaaggca agccttacca
      121 gatgcgcggg tattatggga tgtgcacgaa cccgatggtt gcctgggccg acagtagggc
      181 aacttacgag accttcaaga gtctcgattt ctcagccgtc gcggagatct tcatgacgcc
      241 gacggcagcc atggcggaca tcgttctccc cgtggcccac cagtacgaga tgaacgatat
      301 cggccactac ggcatcggcc acggcatcgt cctcgcgagg ccgaaggtcg tcgaaccgcc
      361 tgaagagtgc tggcccgaca tgaagatcct gaacgagatc ggcaaaaggg tctccccgcc
      421 ggaactatgg cacgaggacc acgaagaatt tctggaggac ctccttcggc cggcgggttt
      481 gacgtaccgt gagttcgtcg agaaggggta cctgaaaggt ccggacagct ttcgaacata
      541 cgagacgaaa ggttttccga cacctaccgg taaggtggaa ttgcgcctga gcacggccga
      601 gaagtttaag ctcaaggcgc tccctgaatt cacgggattt cccgaagaag acgacccaga
      661 ctatccattg ctcgtcacca gcgccaaaag ccgctactat ctcttatctt cgtaccgatg
      721 ggtggcgagg ttgcgcgaga agagaccgca cccgctggtg gaaatccacc cggaaacggc
      781 tgctcgctac gggatcgcag acggggacga ggtcgtcatt gagacgaaat acggcgcgat
      841 aacgcagagg gcccgtgtta ccgacaccat ccactcccgc gtagtcagcg ctgccctggg
      901 ttggtggttc cccgaggccg ccccggagac ccagtatgac tggcgaaggt ccaatttcaa
      961 catgctgacc tccatgaaga aactggggaa ggagttcggc acacccaaca tcacgaacct
     1021 gccctgccgg atacggaggg cgtcggcttg acggattgga tgctctcggc acctagggtc
     1081 gcctcggttt cgttgtgggt tactgaacgt atttgacatt ccccaggccc tgcgggataa
     1141 ttttttcatg aaggttgatc atcgccgcaa gatcattgat gttttcagcg atatcgattc
     1201 ccaccgcgtg accgctgaga tcatcagaag acattcgaca aaccgacgag atgtcagaga
     1261 ggtcgccctt gaaggactcg acctttcgcg tgtccggaag attgtcgagc tgggatgcgg
     1321 gttcggtttt tttaccgagg ccctgagggc gagggtgtcg cctggtgccc gcatcatcgg
     1381 tatcgacgtc gtggagggat acagggatgt cttcatggag gcctgcgccc gggcgggaat
     1441 agacgggaca ttcatccatg gggatgcctc cgtcatccgg gactttgagg accgttcttt
     1501 cgatgccgta atctgcagct attcgcttta ttttttcccc catatcatcg gcgaagtagc
     1561 gaggattgtt gatgaagacg cggttctcgt cgcgacggtg cataacagcg atagcctgaa
     1621 agagcttgtc gatttcaccc gggatatgct ggtcgaaaac ggcatgatcg accgagcggc
     1681 cacctttccc atcgaagcgc tcataaccgg tttttcttca gaaaacggct atgaacttct
     1741 cgagccctgg ttcggtgcta tcattcggaa ggcctatgag aacacccttg tcttcgaggc
     1801 gggggatatg tactccctga tggaatactt caggtttaag tggccgttgt ttctctccgg
     1861 cctgtcgccg cgggatgtgg caagggcgtt cgacctgttt gaggtatatt tacagaggca
     1921 cttcaaccgg agcagagacg gatttcgcat cacgaaagat gacacgattt tcgtctgttc
     1981 caaaccgcgg tgtcggagag atgaacgatg aagagggcgg agcggcgata ctgcgtccat
     2041 tgcggcgagt cggttgccaa gcgggatgaa gacggggtgc tccgcgatta ctgcgccgcc
     2101 tgcgatgtat tcttctatga caaccccctt cccgtggtct cgacgatcct gcaggaggat
     2161 cggaggatcc ttctcgtcaa gaggaaatac aggccgtaca agggcacgtg gtgtcttccg
     2221 tcggggttcg ccgaggtcgg agagggaata gaggacgcgg cgataagaga actcgaggaa
     2281 gagaccggca taaaggggaa gatagtaagt cttgtcgatg ttgattcgtg cagcaattat
     2341 ttctacggtg acttgatatt cctgacattc gaggcgctgc gggtggcggg gaaaatcacg
     2401 cccgggagcg atacagtcgc ggtgaaatac tatcccctag ataagatccc ccgtctcgcc
     2461 tttcggtcta acatgaaggc ggtccggacc ttcatccaga acaaatccga ctattgggcg
     2521 atcattgact ccttctcggc agcagtcgag aagcgtgggg ggcagagaca gaagaagaat
     2581 ctcctgtccg acaggctggt cgagaccatc cgggaaaatg ccgatgccat cgtcgccaac
     2641 tggctccatg atgtttcggt gaataggtcg acccccaact accattccta tgaccctgaa
     2701 aggcttcgcc gtcgcgccca agacgtgctc gagcttttca gcacttgggc cgatgaattt
     2761 tacaaggaca gcgcccagcg gagtgtggac atccgggggt atttcagggg attcggaagg
     2821 gaaaaacgga aggaagggtt tactctgcac gaagcattga gtgcgctcag tctgaagaga
     2881 aagcatatct gggagttcgc cctttcttac ggcatgacaa aaaaaccgct cgacatctac
     2941 atggccttcg aattcgaccg ccggatgatg attttcttcg acagggcgac gttctactcg
     3001 tcgatgggtt acatggaagt gtaacgacgg tcgatggtgg gagaggatac gagccgggta
     3061 gatgttctca ctctttcgcg gcggcgacat cataatcaca agcaatgagg aggtcttatg
     3121 ttccacgaag agaaatgcga tttttgcggt gagtgcctcg agaggtgtta ttaccttgat
     3181 tttgaccggg aaagcggcag caaggagttc gaaaagcttg tcaggggtga aaaggtcaac
     3241 tggctcaagg actgcataac ctgcatcgcc tgcaatgaat actgcccgaa aggcgcccgt
     3301 ccttttgatc tgatcttgcg gcggctggag gaaacagggg atttcaccga cccggcacta
     3361 cgcgcgcaga tggcagagag gttcaagccg gaaggggaac cgaggccggt cgagaaaaag
     3421 gaccgtgtca tgtccatctg cgtcatgcag ggcaacatgc cctgggcgat ccagggacag
     3481 ctcttcgagg ggatacccct tctcaagggt aaacattatt tttgcaatgt cctttttgcc
     3541 catatgggtg acgaatccat catgcgcgac aggctgcggg ctatggtgga caatctcgca
     3601 aggtcagggg ccaaggaaat cgtctttgtc cacgacgact gttacggcct gctgaaaggt
     3661 gtcgccccgg aatacggcat caccttaccc ttcaggcccg ttcatatctt cgagtacctt
     3721 agggattatc tcaagactca caaggacaag gtcaagaaac tggacatgaa ggttgcctac
     3781 cagcgtccct gcgcctcgcg cttaacgcct gagaaggatg ggcttctgga cgagatattc
     3841 gacctcatcg gcgtccggcg cgtaaagcgg cagtacgacg ggatagatgc gctctgctgc
     3901 ggcgtggaag tcgccggtcc cggcctcaaa ctctttcccc ggggaaagaa cttcgcgcct
     3961 ttccgggaaa agaacgtgaa agatgccaag gacagcgggg cggaggcaat ggtgtacctc
     4021 tgtccgatgt gttttgcgac gctgaacaag ccggtgagag aggcggggat gaagaattac
     4081 atgataagcg atatctgccg gctggccctc ggcgagacgc tccccccgga aaaaccgctg
     4141 taaaacgggt cgagcccgcc caggcactcc tccctggaga caaccggtga gctcgttgac
     4201 cctgaaaaat cggctttaag gaggtaaaaa tgaaggctgc gaaccgaaaa aaagagtctg
     4261 tagaagggat atcccctgat gagttgttgg cgggtatggg tgttacgccg gcggacaacg
     4321 ccgagggtct caccaccgcc atcccctgga ggagtctgat ggggggtgag ttgacgaggg
     4381 gcctcttttg ctggaagcgt tgcctcgaag cagtcggtga aaaccccttt cttttcggtc
     4441 gccttgctat gacagcagcc gggcagaagg tcgtgctcga atcgcaggta accgagccca
     4501 tcaaaaaata cgcccggctt gccgttgtga cgaaaaagaa ccccgatgtt ttcggggagc
     4561 tgaatgcctg gatatacaag agctacgccc agatgctgta caacttttgc ggcaacttca
     4621 tgacggacaa tcgttttgac cggcaaaaag cccggtacac ggtgacgact ccggacggga
     4681 aggaattctt gaaacgtacc ctcgccgaat tcgcattcct ggaaaacaac tacaattccc
     4741 gcgggcttac tcgtctgcac gccatgaagg gtctcttgaa atgcttcctt gttctcatca
     4801 ccggcaagcc tttgcaaaac ggggagatgc tcttttggag aaaaaagagt ggcggtaccc
     4861 cttctatttc cttcgaagaa tacctcgagg aatgccgcgt ccgttttgag aacctctaca
     4921 cgaacgacgc tttcgacgtg cgggactatt ccgaccgggc gacgggaggc atgatcgggt
     4981 atatgccgta cagcgttgtc gaggggtcga gtctccattc ggtgaccctc cgccactatc
     5041 caaggccgga gggtatccca ccgaacggaa aggtcctcta tctggcgtcg ccgctcataa
     5101 acaaacccga aatatacgac cttgcaaagg gcaagtctgt gatcgagggt atgttgaggc
     5161 tggggtatga cctctatctc gtggatcacg gagagccagg ctacagagag tcgaaactgg
     5221 gcctcgattt ttacggaaaa acagtccatg accggtacct cgacatctta gagcgcctcc
     5281 atccggggag tgagatccat accatgggct actgtatggg agggacgttg ctcatttctt
     5341 acctggcgag acgggccgag gagcggatgg ccttgggtct tccgatggac atccgtaagg
     5401 ttgccctgct tgctacaccg gtcaagtggg acgacgaaga atcggggatg cgtcctatgc
     5461 gggatgtcat ccgtaattat tacgacccgt atctgatgag agagctgttt ggggacgtga
     5521 acgttccccc gcagctgatc gagatgggga tgaatctcac ccaaccgggg gttcagtttt
     5581 atatggcgag cggtctttac gagagggccc atattcctga cgacttggaa gattcggcgc
     5641 cttttctcta ctgggtgacg cacggcacga agttcccgga gcgtgcccac gaagagtgga
     5701 tcgcgaagat gtacatgggc aaccagatct acgaaggaac atactgtctg ccctcatccg
     5761 tgaaggcatt ggacggaaag ccggtgaaca tgaacgccct gaaagacgcc ggtgtcgcga
     5821 tcttcaacta ccggggcgac cgggacgtga tcgcaccgcc gggctcctgc gtggccggcg
     5881 atttgtgggg ggtcacggag gagggtaaca tttccgtgag tcgaggcggt ttgaaccgga
     5941 ccatcgagaa gcatgccggc cacatcttcg tagtaagcag aaccctcctg gcggagtttc
     6001 tcgacctcgt ctccgccttt ttcaaggggt gatggagacg cctgtgcgaa gaaggtggaa
     6061 tccggcctgc aggccgagga agttacactc atggtaatgc ctagggaccg gttcgtcacg
     6121 gtaaacaatg tcgagacccg ctactgggct gtcggcgacg gaaagaggcc gctcgtcctc
     6181 atccacggcc ttggtgcatc ggcggagatc tggatgcaca acatcggggc gttttcggag
     6241 aaccataggg tgatcgtccc cgaccttccc gggttcggcc gaacagacat gcccggcgag
     6301 cccttcgcgc ccttcgatta tctctccttc ctggacggtt ttctcaggga actcaaagtc
     6361 gagcgagcgg tactttgcgg acagtctctc ggcgggggca tcgccctcct gtatgccatc
     6421 cggtttcctg agaaggtcga gaaactggtt ctggtggatt gtgccggtct cggagtggaa
     6481 gtcatctgga cgctgcggtg gatgacgttg cccttgatcg gggagctgtc ttccgtaccc
     6541 actcgcccgg gtgtggcgct cttcttcaag ctggccgtga aaaatcccgc tgttgtgacc
     6601 gatgaactga tcgacctcta tcacggatat ttcaatcgtc ccggccgacc ggcgtttctg
     6661 ctgaggctcg tccgttctct cgtcgatttc cgccgtgtaa agcgagatgt cgtctcaccg
     6721 atcctggaaa atcttcaccg ggtcgcctgt cccaccctga tcgtttgggg tgagcgtgac
     6781 cgggtctttc ccctctctca tggccgtcac ggcctggaaa ggataaaggg ggccgagatc
     6841 cgggtcatga gagactgcgg ccatatcccc ttcttcgagc ggccggggga gttcaacgaa
     6901 ctggttctcg attttttact gcctccgctg tcctgtgagg gagatcaggc aagaccgttt
     6961 gacctgtaag gggaagaacc gcagtgtcag atgttagtcc tgtagccggc gccgatagtg
     7021 ctgagggcct tttcggcggc ttcggagagt tccctgtcgt ggtcgtggtt gagggcgaag
     7081 acacggaagt ggacgacccg ggcggggata aagcggtaga tgtcctggag cggttccggc
     7141 gaggtgaggc cggtgagggg gttgaagacg ttgatacgtt tttcctcttc tgccatgggg
     7201 ttcaggggcc gcgggtcctg agtggcgagg tcgacacgaa actcgagatt tttcaggtcc
     7261 ttcgccagga actggcgtat ctccttctcg tagtcatcgg ccgtcgagaa tcgtgcaccc
     7321 tttcgtacct cgtcaataga gagctcggcg gcgaaggcca tcttccattt tacggccctt
     7381 gagtggatcg tctcccattc tttccccagc ctacgttttg ccgccgacct cgcctgcggc
     7441 caggcgcgca cctgttcgaa gaggtgccag tcgtcgagct tgagatattc gtcgagttcc
     7501 tcaagagggt gatggggaaa gaggatatcc atcgtctcgc ggaagatttc ctgcaagtga
     7561 aggtcgaggg cccttgtggt tcggtggtag tagacgtttg tgtagaggtt gagccttgcg
     7621 ttaaggaagc ggctgagggc cgagataccc gcctggtgga gcgtgaggcc ctttccggtg
     7681 aagaaggagt aaaacctgag ccgcgagatg tccacgatgt ccatggaaaa ccccgtcatg
     7741 taagcatcgc gctggacgta gtcaaggttg tccacggtgt aaacaccgga aaagagctgg
     7801 cgtagcatct tgagccattg cgggtgccgt cggtcaccct cctccggcat ctttatcagg
     7861 aaggagatct gctcaggctg gatggtctca cggccctcga agggacccgt ggggcttcgc
     7921 cgcatccctc tgatgatccc ggcgagtttt ctcttgatta tctcctggcc aatccgctcg
     7981 tggttaaggt cgaattggcg gatgaactcg tcgtcgaaga agtggccgta agggccgtgc
     8041 cccacgtcgt ggaggagtcc cgccacacgg agcaactctt cgacgaaatt gagggacggg
     8101 acatcgccgc agacctcctt caggctcgga tagaggtaac ggccgaattc accggccatg
     8161 tgcatcgttc ccagggagtg ctgaaaccgg ctgtgctctg ccgcggggta gacccagcgg
     8221 gcgctctgga gctgattgat ccgccgcagg cgctggaccc agggagaatc gatcaggtcc
     8281 ttttccgtcg attcctttgg gtagtccggg gacgggacgg tgaaccaggc gtactggtat
     8341 atcgggtcgg cgatcagcgc cttgccctgg taagcggagg cgtattcggc cttctttctc
     8401 ttcacggcta ttttttcttt cccgtgacgg cgatgatgtt gaacgaggaa gaccaggtca
     8461 ccggtttcag gacggctttc ccgccgatgc tcttcgcgat ctctttggcc tccttttccg
     8521 cgtcggcagt atagtagata gtatcctccg cgaaattgga ccgaggtgcg tggtcgacga
     8581 tctctacttt gaggcccgtc ttctctatca gctgcgccat ctttctcgca gaggccagcc
     8641 ttccgtcgcc cgacagtacc ttgatccgca agggtttagc cagggtttct ccctgggatg
     8701 ccgcctccgc cttccgtacc tcttcctttt ctgttatcgc ttcggtcttg atcgctgcgg
     8761 ctttttcacc ctgcctctgc ttttctatgt actcctggag gtctttgacc tgagccgtca
     8821 aggccgcctt ctcgctctgc agctttgcga cctcctcttt agccgattgg agtgccttgt
     8881 ccttctcttt gacatccgcc tcgagacgtg tgagttcctt tttcatctcg tcgttcgtga
     8941 tggtcagtac ctgtatctgt ctctcacaac ttccgtccag gcacttgagc gaagcacaac
     9001 ctgaaaaaaa gaaaaccgtg acgatgccga ttatcatcca ctgtagtttt ccgcccatga
     9061 ttccctcctt tccggcaaaa cgccgcaaca tcattgtatt tttaaatatc gtaaacgacg
     9121 gaaaaaatcc atagaaatat ttgatgtttg tgatagattg cgcggcatga gacttcctat
     9181 gctcagagcg tttttcttgg tggcggcgat ttttgccggt ctttcg
//
LOCUS       DAOTMX010000025         8724 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_7308_length_8724_cov_3.584843, whole genome shotgun sequence.
ACCESSION   DAOTMX010000025 DAOTMX010000000
VERSION     DAOTMX010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 8724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8724
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7308_length_8724_cov_3.584843"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..943
                     /locus_tag="P5573_01370"
     CDS             <1..943
                     /locus_tag="P5573_01370"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRT26748.1"
                     /translation="SLDSGTGAKQLQGRHFTEFVAPEHKEFLMKRWEEIKGGQYHPFE
                     IEATARDGTKRNLLITPRPIKGTDRYLFVQRDITEFKDLEKKFYDSQKLAAVGQLSAG
                     IAHEVRNPLSSIKMSLQILQKRLRPEGNDLKRFKIAEKEVEHLEKIVSDILLFAKPME
                     PDMRPGDVNSFIDSSLLMAEKEILEKKIEVRADYDSDIPQANYDPGMLRQALLNLYLN
                     AIDAMDEGGVLTISTRFVRKGDGEWVLIEIEDNGSGIDEEDIPHLFNPFFTRKKYGTG
                     LGLAQVKKIIDLHRGTIEIVSVKGRGTKVSVALPAGG"
     gene            1090..2460
                     /locus_tag="P5573_01375"
     CDS             1090..2460
                     /locus_tag="P5573_01375"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRT26749.1"
                     /translation="MAKIIVIDDDRSISESLELYLSEEGHEVMTANTGTDGLNLVLQT
                     SPDIVILDIRLPDIDGFTVLEDLKEENVDAKVIMITAHHDMETTIKAMKEGAFDYIHK
                     PINIDELDIAIQKAINTLEMEKKINGLLTEPSRSFKVGDIIGTGKAMLDIFKVIGVVT
                     QSKTTVLIQGESGTGKELIAKVIHNNTSPTEPYIAINCSAIVDTLLESELFGHEKGSF
                     TGAINRKPGKFELARHGTVFLDEISEMSLNLQAKLLRVLQEMEFERVGGKDRVKVNAR
                     IIAATNKDLKTLVEEGKFRDDLYYRLNIVTINIPPLRERREDIPLLVDYLLKKINRDL
                     HKNIVGVSKEMMDVFMKYGWPGNVRELENILVRAAVVAKGQVLGREDFPDLLGSAQVP
                     KAPAALAGEEEEGTGFETLDEIEERHIRRVIAKVGRNKGEICEILGISRPTLERKLEK
                     YGIRFD"
     gene            2522..3118
                     /gene="yihA"
                     /locus_tag="P5573_01380"
     CDS             2522..3118
                     /gene="yihA"
                     /locus_tag="P5573_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HRT26750.1"
                     /translation="MKVTSAEFVKSAVEPSQYPDSPYPEVAFAGRSNAGKSSLINALV
                     NRRKLAQTSTTPGKTRLLNFFVVNRRLSFVDLPGYGFANVSRSLRSAWRPMVEDYLRQ
                     RKNLCLVVLVLDVRRGVSEEDIALAGWLRRHDRDFLVVLTKSDKVSKEEAKRRLADAA
                     EVLGLSGDQVLLFSAVKGLGKEELWREMGKKITFAKLA"
     gene            complement(3222..4061)
                     /locus_tag="P5573_01385"
     CDS             complement(3222..4061)
                     /locus_tag="P5573_01385"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HRT26751.1"
                     /translation="MQNPYVPYPVEVIKIVTEVDTKDIKTFRLAFVNKEDEESFKYLP
                     GQFAELSIFGKGESPIGIASSPTNPGYIEFTVQKAGVVTTALHEMEEGTMMGVRGPLG
                     NSWPIEYLEGKNIVVVGGGFAFTTLRALIQYMLNDSNRKRFGKITTIYGARTPGLLLY
                     KDELEAWSKRDDMELHVTVDKGDETWKGREGFVPTVCKEVAPSAEDAVTVICGPPIMI
                     RFTLPVFFELGFSKENIYTSLEMRMKCGIGKCGRCNVGSKYVCKDGPVFSLAELDRLT
                     PEY"
     gene            complement(4075..5100)
                     /locus_tag="P5573_01390"
     CDS             complement(4075..5100)
                     /locus_tag="P5573_01390"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRT26752.1"
                     /translation="MEKRLIKKDELLGIVKKVGGEFNVFAPVKLEDNVLFKPLAPGEE
                     PLLTFANTKNAPKGVFFPRTEVMIRYVRTPKGTVLKGVELEARDAVLFGVRPCDARSF
                     VLLDLLFDQEKYKDPYYIAKREKATVVTLACTPVPYTTCFCTSVGGGPVDSTGSDVLV
                     TDIGDVYLVEFLTPKGERLAKYFGEAKATAEADNKKETAAKEAVGQIKSNIPAQEIKP
                     ILDVSFDHPFWDTIHKRCLACGTCTYLCPTCHCFDINDEMKYDDGRRLRNWDSCMFPL
                     FTKETSGHNPRPTQKERWRQRVMHKFKYYVDNFNAIACVGCGRCVMYCPVNIDIRKIV
                     EDISKLQ"
     gene            complement(5127..5978)
                     /locus_tag="P5573_01395"
     CDS             complement(5127..5978)
                     /locus_tag="P5573_01395"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HRT26753.1"
                     /translation="MKVSYYPGCSLHGTAKEYDQSVKAVSSALGIDLAEVEDWSCCGA
                     TSAHSTNFKLSVALPARNLVLIESAGDKEVMVPCAACFNRFKSAQHHLAHDDRLKAAI
                     DGIIGRPYQGTVTVKHPIGLFHDDVGMEAIAQKVVRRLTGLKPVSYYGCLLVRPPEVC
                     QFENYENPYMLDEILGTLGAEVKQWSFKTDCCGGSLTISRTDVVRNLVDRLMTMAREA
                     DANCVVTACPVCMANLDTRAGDNVKLPVFYFTELMALAMGIPGPADWFKLHMVDPTPL
                     MSGLGLM"
     gene            complement(5998..6522)
                     /locus_tag="P5573_01400"
     CDS             complement(5998..6522)
                     /locus_tag="P5573_01400"
                     /inference="COORDINATES: protein motif:HMM:NF024924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRT26754.1"
                     /translation="MKTFLEEVNEKISGVPIQRCYHCRKCTAGCPLASVMEYNPNRVI
                     KMIQMGMKKEVLNSSTIWLCASCETCITRCPNEVDIARMMDVLREMALKEGVPAKEKN
                     VVLFHEAFLRGIKLRGRINEPLMMVNYKLRSGDLFSDMSMGVEMLLKGKLPLISPKTK
                     NLRAVKEIFEKTRQ"
     gene            complement(6541..7587)
                     /locus_tag="P5573_01405"
     CDS             complement(6541..7587)
                     /locus_tag="P5573_01405"
                     /inference="COORDINATES: protein motif:HMM:NF024924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRT26755.1"
                     /translation="MTGRDQQNEDSRKVEAIEKKLREEAKKLLEEKKVDVIVGYEAGT
                     LPLTAVPTFVTDPSDTAKLLWNALCVQNLAKYVHDVIFQHRNAQKRVKPENRTKKVVG
                     VVARGCTTRSIILHLQERQYTREEIVVLGVPCRGYVDKKKLSVLLEGQEIVDGALDGE
                     QLKVTTAAGDRIVAVADVLADNCLTCRFNNPVINDLTVGEPAPAMRADEEYDAVKAFE
                     SRPSEERWAYFEKEMAKCIRCYACRQACPSCYCPECFAEQSRPQWVGIGENAPDTQVF
                     QIMRIFHMAGRCVDCGSCVAVCPMGVDLRTFLKKLDKDAYELFGSRAGASLDDPPVLA
                     TFREHECEDFIYNP"
     gene            complement(7584..8021)
                     /locus_tag="P5573_01410"
     CDS             complement(7584..8021)
                     /locus_tag="P5573_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HRT26756.1"
                     /translation="MSEDRVKEGWDPKIAAIVCNWCTYAGADLAGISRIQYPPNVRII
                     CVPCTGRINPFYIVRALQEGADGVLVSGCHPGECHYITGNLSARRKFAMLKRFLDYLG
                     LEADRTTFTWCSASEGERFAKVVSGVTERVRQLGPAKKLVKSL"
     gene            complement(8091..>8724)
                     /locus_tag="P5573_01415"
     CDS             complement(8091..>8724)
                     /locus_tag="P5573_01415"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRT26757.1"
                     /translation="EDFYKKARELNVKFIRYDEDRKPEISQAGDRLVVRAFDPILNGS
                     VELPADLLALSVGTVPNPENAAIGQMLKVPTNQDGFFLEAHVKLRPVDFATDGVFMCG
                     MAHAPKLSEDTIVQANAAVSRACTVLTKDFIEAEGKTAYVIKERCSGCGLCEQNCPFG
                     AIAVNPVEGVAEVNTVLCKGCGVCTASCRMNAVDLNGFTNEEVIAQIAAL"
BASE COUNT         1994 a         2411 c         2305 g         2014 t
ORIGIN      
        1 cagcctagac tccggcacag gagcaaagca acttcaaggg aggcatttta cggagtttgt
       61 ggcgcccgag cacaaagagt tcctcatgaa aaggtgggaa gagatcaaag gaggtcaata
      121 ccatcctttc gagatagagg cgacagcgag agacgggacg aaacgaaacc tgttgatcac
      181 cccaaggcct atcaagggaa cggaccgtta tctgttcgtc cagcgggata tcactgaatt
      241 caaggacctg gagaagaaat tttacgacag ccagaaactc gcggcggtgg gacaactctc
      301 ggcaggcatc gcccacgagg tgagaaaccc gctttcgtcg atcaaaatga gccttcagat
      361 cctgcaaaag cgacttcgcc cggaaggcaa tgacctaaag aggttcaaga ttgccgagaa
      421 agaagtggag catctggaaa agatcgtcag tgacatactg ctcttcgcta aacccatgga
      481 accggacatg aggccgggag acgtcaactc cttcatcgac agttctttgt tgatggcgga
      541 gaaagagata ttggaaaaga agatcgaggt ccgcgccgat tacgacagcg atatcccgca
      601 ggcaaactac gaccccggga tgctcaggca ggcgctcctg aacctctacc taaacgccat
      661 agatgcgatg gatgaaggag gagtactaac catctcgaca cgttttgtca ggaaaggtga
      721 tggtgaatgg gtgctgatag agatcgagga caacgggtcc ggtatcgacg aagaggacat
      781 cccccacctc tttaacccgt ttttcactcg gaagaagtat gggaccgggt tgggattggc
      841 acaggtaaag aaaatcatcg acctccatcg gggtacaata gaaatcgtca gcgtgaaggg
      901 ccgggggacg aaggtgtctg ttgcgctacc ggcaggcggg taacaataga gggtatacgg
      961 tgaagggcaa acagtaaacg gtggtttatg gggtgaacgg agcaacgggt tgagatgaca
     1021 tgaaaaatgt ctttcaactt tttgtgagtt cgtcgccgct tattccaact gctcactttc
     1081 agggacgtta tggccaagat cattgtgatt gacgatgaca ggtcgatcag tgaatccctc
     1141 gagttgtatc tctcggagga ggggcatgag gtaatgacgg ccaatacagg aacggacggc
     1201 ctaaacttgg tcctccagac gtcgccggac atcgtcatcc tggacatccg actgcccgac
     1261 atcgacgggt ttaccgtcct tgaagacctg aaagaggaga acgtggatgc caaggtgata
     1321 atgataaccg cccaccacga catggagacc acgatcaagg ccatgaagga aggggccttt
     1381 gactacatcc ataaacccat aaacatagat gaattggaca tcgctatcca gaaggccatc
     1441 aatacgttgg agatggagaa gaagatcaac ggccttctca cggaaccatc ccggagcttc
     1501 aaggtcggcg acataatcgg caccgggaag gccatgctgg atatcttcaa agtaatcggc
     1561 gtggtcaccc agagcaagac caccgtcctt atccagggcg agagtggaac cggcaaggaa
     1621 ctgatcgcca aggtgatcca caacaatact tctcccaccg agccttatat cgccataaat
     1681 tgctcggcca tcgtagacac cttgctcgag tcggaactct tcgggcatga gaaggggtct
     1741 ttcacggggg ccatcaaccg gaagccgggg aagttcgaac ttgcccgcca tggcaccgtc
     1801 tttctggatg agatcagtga gatgtccctg aacctccagg caaaactcct cagggttctc
     1861 caggagatgg aattcgaacg ggtggggggc aaagaccgcg tcaaggtgaa cgcgaggata
     1921 atcgccgcca cgaataagga cctgaagacc ctggtcgagg aaggtaagtt cagggacgac
     1981 ctgtattatc gcctcaacat cgtcacgatc aacatccctc ccctcaggga gaggagggag
     2041 gacatcccct tgctcgtcga ttatctgctg aagaagataa accgggatct ccacaagaac
     2101 atcgtcggcg tttcgaaaga aatgatggac gtcttcatga aatacggttg gcccggtaac
     2161 gtccgtgaac tcgagaatat ccttgtacgt gcggccgtcg tcgccaaagg gcaggtgctc
     2221 gggcgcgagg atttccccga tttgctgggt agcgcgcagg taccgaaggc accggcggcg
     2281 cttgccgggg aagaagagga agggaccggt tttgagaccc ttgacgagat agaggaacgt
     2341 catatccgca gagtcatcgc caaggtgggc aggaacaaag gtgaaatctg tgagatcctg
     2401 ggtatatcac gccctaccct cgaaagaaaa cttgagaaat acggtatccg tttcgattga
     2461 agggcgaagg gcgggcggct gagaatgatc ttttacgaat agctgaaagc ccaaatttta
     2521 catgaaagtc acatccgcag aatttgttaa gagtgcagta gaaccgtccc agtaccccga
     2581 ttctccttat cccgaggtcg ctttcgccgg aaggtccaat gcgggcaagt cctcgttgat
     2641 caatgccctt gtcaatcgca gaaaactggc ccagacaagc acgacaccgg gtaagacccg
     2701 cctgctcaac tttttcgtcg taaaccggag gctgtctttt gtggacttgc cgggatacgg
     2761 ttttgcgaat gtctcccgta gcctccggag tgcctggagg ccgatggtgg aagattactt
     2821 gcggcagagg aagaacctct gcctcgtcgt cctcgttcta gacgtccggc gaggcgtctc
     2881 cgaagaagat atcgctcttg ccgggtggct ccgccgtcac gacagggatt tcctcgtcgt
     2941 cctgacgaag agcgataagg tatcaaaaga ggaggcgaag cggcggttgg cggatgccgc
     3001 ggaggtttta ggactatccg gggaccaggt tcttctcttt tctgctgtga aaggcctggg
     3061 gaaagaggag ctatggaggg aaatgggaaa aaagattact ttcgccaagt tggcctgaga
     3121 tgggaagcat ttctttctcg catgtagtag aacgcctggg ctatcccacc gctcccatgg
     3181 acatcatcct ttctaaatct catctgtaga ggcttaaggt atcaatattc cggtgtcagt
     3241 ctgtccagct cggcaagcga gaatacaggc ccgtctttgc atacatactt actacccaca
     3301 ttgcagcgcc cacacttgcc tataccgcat ttcatccgca tttccaaaga ggtatagatg
     3361 ttttccttgg aaaacccgag ttcgaagaac acgggcaagg taaagcggat catgatggga
     3421 ggaccgcaga tcacagtcac cgcgtcttct gcactcgggg cgacttcttt gcaaacggta
     3481 gggacaaagc cttcccgccc tttccaggtc tcgtccccct tgtccaccgt gacgtggagc
     3541 tccatatcgt ctcgcttcga ccaggcctcg agctcatcct tgtaaaggag gagtccgggc
     3601 gtcctcgccc cgtagatcgt cgtgatcttg ccgaagcgtt tgcggttgct gtcgtttaac
     3661 atgtactgga tgagggcgcg caacgtagtg aaggcaaatc caccgcctac caccacgatg
     3721 ttcttgccct ccaagtattc gatgggccac gaatttccta ggggtccgcg gacacccatc
     3781 atggtccctt cttccatctc atgcagtgcc gtagttacta cgcccgcctt ttgcacggta
     3841 aattctatgt agccggggtt ggtcggtgac gaggcgatgc cgatgggcga ctcacccttt
     3901 ccgaagatgg agagctcggc gaattggccg ggcagatact tgaaactttc ctcgtcttcc
     3961 ttgtttacga aagccagtcg aaaggtcttg atatcttttg tatccacctc agtcacgatc
     4021 tttatgactt cgaccggata gggtacatat ggattctgca taacgttctc ccgactactg
     4081 taatttcgaa atatcttcga cgatcttccg aatatcgata ttgacaggac agtacatcac
     4141 gcatctgccg catcccacac aggcaatggc attaaaattg tccacgtagt atttgaattt
     4201 atgcataacc cgctggcgcc agcgctcctt ttgcgtcggc cgggggttgt ggccggacgt
     4261 ttctttagtg aacaggggaa acatgcagga gtcccagttc cgaagacgac gcccgtcatc
     4321 gtatttcatc tcatcgttta tgtcgaagca atggcaggtg ggacaaaggt acgtacaggt
     4381 accgcaggct agacaccgtt tatggattgt gtcccagaag gggtggtcaa aactcacgtc
     4441 aaggatgggc ttgatttcct gggcggggat gttggatttt atctgcccga ccgcctcctt
     4501 ggcagccgtc tccttcttgt tgtccgcctc ggccgttgcc tttgcctccc caaagtactt
     4561 ggccagcctt tcccccttgg gcgtcagaaa ctccacgagg tatacgtccc cgatgtcggt
     4621 aacgaggaca tcgcttcccg tactgtctac agggccgccg cccacagagg tacaaaaaca
     4681 cgtggtgtaa ggaacgggtg tgcaggcaag agttaccacg gtcgctttct cgcgtttggc
     4741 gatgtaatat gggtctttgt atttctcctg gtcgaataaa aggtcgagga ggacgaaact
     4801 ccttgcgtcg caaggcctga caccgaagag caccgcatcc cttgcctcta actccacccc
     4861 tttcagtacc gttcccttag gagtacggac gtaccggatc attacttccg tcctggggaa
     4921 gaacaccccc ttgggagcgt tcttagtgtt ggcaaaggtt agtaacggtt cttctcccgg
     4981 tgcgagcggc ttgaacagga cattgtcttc taatttcacc ggggcaaaga cgttgaactc
     5041 tcctccgacc ttctttacga tccctaacag ctcatctttt ttgatcaacc gtttttccat
     5101 ggtatccttc agtcttccaa gtttttttac atcagcccca aaccactcat gagcggtgtg
     5161 ggatcgacca tgtggagttt gaaccaatcc gccggtcctg gtattcccat cgccagggcc
     5221 atgagctcgg tgaagtaaaa taccggcagc ttgacgttat caccggccct cgtatcaaga
     5281 ttcgccatac agaccggaca ggccgtcacg acacagttgg cgtccgcctc ccgagccatc
     5341 gtcatcagcc ggtcaaccaa gttccgtacg acgtctgtcc ggctgatcgt caggctgccg
     5401 ccgcaacaat ccgtcttgaa ggaccactgt ttcacttctg cccccagggt ccctaagatt
     5461 tcatccaaca tataaggatt ctcgtagttt tcgaactggc atacctcggg cggcctgacc
     5521 aacaggcaac catagtacga taccggtttt agaccggtca gccttctcac gaccttttga
     5581 gcaatcgcct ccatgccgac atcgtcatgg aaaagaccga tgggatgctt taccgttacc
     5641 gttccttgat atggtcggcc gatgatgccg tctatcgccg ctttcaatct atcatcatga
     5701 gcaagatggt gctgggcgct cttgaatctg ttgaaacagg cggcgcaggg taccatcacc
     5761 tccttgtccc ctgccgactc gatcaatacg aggtttcgtg cgggcaacgc cacggatagc
     5821 ttgaaattgg tagagtgggc cgaagtggca ccgcagcacg accagtcttc tacctccgcg
     5881 aggtctatcc ccagtgcaga agacaccgcc ttcacagact gatcgtactc cttcgctgtt
     5941 ccatgcagag agcatcccgg atagtatgaa actttcaaca cttgcctcct tatgagatca
     6001 ctgtctggtc ttctcgaaaa tctcctttac cgccctcaag ttcttggtct tgggagagat
     6061 cagggggagt tttcctttga gaagcatttc gacgcccatc gacatatcgg aaaataggtc
     6121 tcccgaccgt agcttgtaat taaccatcat gagaggttcg tttatccggc cacggagttt
     6181 gatgccccgc agaaaggcct catggaagag taccacgttc ttttctttcg cggggacacc
     6241 ctcctttaag gccatttccc tcagaacatc catcatcctc gcgatatcca cctcgttggg
     6301 gcatcgggtg atgcacgtct cgcaggatgc gcataaccag atggtcgaac tgttcagcac
     6361 ttccttcttc atccccatct ggatcatctt tatgacccga ttggggttgt actccatgac
     6421 cgatgccaga gggcatccgg ccgtgcactt ccgacaatgg tagcatcgct ggataggtac
     6481 accgctaatc ttttcgttca cttcttcgag aaaggtcttc attatgcact ccgtttcacc
     6541 ttacgggttg tagataaagt cctcgcattc gtgctcccgg aaggttgcga gcacgggggg
     6601 gtcatccaac gaggcgccgg cccgactccc gaagagctca taggcgtcct tgtccaattt
     6661 tttcaggaag gtcctcagat cgacccccat gggacagacg gcgacacagg aaccgcagtc
     6721 cacgcaccgc cccgccatgt ggaaaatcct catgatttgg aatacctgcg tatcgggggc
     6781 attttctcct atgcctaccc attgaggtct gctttgttcg gcaaagcatt ccgggcaata
     6841 acaggagggg cacgcttgac ggcaggcata acaccggatg cactttgcca tctctttctc
     6901 gaaataggcc cacctctcct cggaaggccg ggattcgaag gccttaacgg cgtcgtattc
     6961 ttcgtccgcc ctcatggccg gcgccggctc acctaccgtc aggtcgttga ttacggggtt
     7021 gttaaaccgg caggtgaggc aattatcggc caggacgtcc gctactgcca cgattctatc
     7081 accggcggct gtagtcacct tcagttgctc gccgtccaag gccccgtcaa cgatttcctg
     7141 tccttcgagg aggacggaaa gcttcttctt atccacatac ccccgacagg gcacgcccag
     7201 gaccacgatc tcctcgcgag tgtactgccg ttcctgcaga tggaggatga tagaacgagt
     7261 cgtacagccg cgcgctacga cacccaccac cttctttgtc ctattttccg gcttcacccg
     7321 tttctgtgcg ttccgatgct ggaagatgac gtcatgcacg tattttgcga ggttctgtac
     7381 gcataaggcg ttccagagga gcttagccgt gtctgaagga tcagtgacaa acgtgggcac
     7441 ggccgtgagc ggcaacgtcc ccgcctcgta acccacgatg acatcgacct ttttttcctc
     7501 taacagtttc ttcgcctctt cccgcagctt tttctcgatg gcttccactt ttctcgaatc
     7561 ctcgttttgt tgatctctac ccgtcacagt gatttaacca gcttctttgc cggccccagt
     7621 tgcctgaccc tttccgttac cccactaact accttggcga atcgctcacc ttcggaagcc
     7681 gaacaccagg taaaggtcgt ccgatccgcc tccaaaccga ggtaatcgag gaatctcttc
     7741 agcatggcaa actttcgccg ggcgctgaga tttccggtta tgtagtggca ttcaccgggg
     7801 tggcaaccgg acacaagaac tccatccgct ccttcctgta gggcgcgcac gatgtaaaaa
     7861 ggattgatcc gtcccgtgca ggggacacag atgatgcgga cattgggggg atactggata
     7921 cgacttatgc cggcgaggtc ggcaccggcg taagtgcacc agttacacac gatggcagct
     7981 atcttcggat cccaaccctc tttcactcgg tcttctgaca tctcgttctc cgtttctgaa
     8041 ccatagtcta ttccgccctt tctccttgtg agggcgcgtc atttgtcacg ctacagggct
     8101 gcaatctgtg caatgacctc ttcattggtg aagccgttca aatcgacggc gttcatgcga
     8161 cacgatgcgg tacagacgcc gcaccccttg cagagcactg tgttgacctc cgccacccct
     8221 tccacgggat ttacggcgat cgccccgaac ggacaattct gttcgcagag cccgcaacct
     8281 gaacagcgtt ccttgattac gtaagccgtt ttcccttccg cttcgatgaa gtccttcgtc
     8341 agcactgtac acgcccggga cacggcggca ttcgcttgga cgatcgtgtc ttcgctgagt
     8401 ttgggggcat gagccatccc gcacatgaag acgccgtcgg tagcaaagtc cacgggccgc
     8461 aacttcacgt gagcctcaag gaagaacccg tcctgattgg tgggcacctt tagcatctga
     8521 ccgatggcgg cgttttcggg gttcggcacc gtacctaccg agagggcaag caggtctgcc
     8581 ggcagttcca ccgacccgtt gagaatgggg tcaaaggccc ggacgaccag tcggtcccca
     8641 gcctggctta tctccggctt gcggtcctcg tcgtagcgga tgaacttgac gttcaactcg
     8701 cgggccttct tgtagaagtc ttcc
//
LOCUS       DAOTMX010000026         8707 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_7325_length_8707_cov_3.920943, whole genome shotgun sequence.
ACCESSION   DAOTMX010000026 DAOTMX010000000
VERSION     DAOTMX010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 8707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8707
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7325_length_8707_cov_3.920943"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..934)
                     /locus_tag="P5573_01420"
     CDS             complement(<1..934)
                     /locus_tag="P5573_01420"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HRT26758.1"
                     /translation="MPSVCCYFQVHQPCRHRHFTFFDIDRDHRYEDDEKNAVILKKVV
                     EKCYLPANRLMLELVRKYKGDFKVAFSITGIVLEQMETHCPEVIDGFKRLADTGCVEF
                     LNETYYHSLAFLFSRREFKDQVELHKRKIRKLFGQTPRTFRNTELIYNDDLAREVEKM
                     NYKVILAEGADQVLGWRSPNFVYRPDGCLKLKLLLKNYRLSDDVAFRFSDRKWKEFPL
                     VADKYAHWIHQINKSGDVVNLFLDYETFGEHQWEDMGIFDFLRAFPREVLKHPDFRFQ
                     TPVEVAEEFEPVAQLSVPRYTSWADMERDLTAWLG"
     gene            complement(1024..3012)
                     /locus_tag="P5573_01425"
     CDS             complement(1024..3012)
                     /locus_tag="P5573_01425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HRT26759.1"
                     /translation="MQFLVGEDICRNIGKSLSLEWLEADGAGGYASSTILMCHTRKYH
                     GLFVPNLADPPGRHVLLSKFEDSVFVKNEEVFLSCHQYPGVFFPGSNHALQGYEFDYC
                     PRFLLKAGGIVIHRRIMLVQGGKCVLVRYSCESGGTLRLRLKPFLAFRGHHRLSRENS
                     FIRRDVSRTAYGFELQPYEGMPGLVIRTDGESEFTPLPLWYKDFEYVREQERGYDFRE
                     DLFMPGLLEMELAQGREVYLSASVRGCETDPQTIWKNEENRRKQVSSIVRSCVQAARS
                     DGERKMLEGAIQSGRCFPVRTPTGRNTVIAGYHWFSDWGRDTLISLPGLTFCSGRPRD
                     GVEILKAIGEHERGGLLPNFFTDDGKAAYNSVDSSLWYFWAVQQMLDHTGDLKTIEES
                     FWPVMKKIIRSFMAGTDHEIYMAGNGLLHAGDSTTQLTWMDAFVDGRPVTPRAGYAVE
                     VNALWYNALCIAKDLSDKFQDGDMDLKDTISNIRDSFEKVFWLTDGSYLADVFTGDGI
                     DRSVRPNQIIAVSLPYRAIDADKCEGVLKKVVSDLLTPYGLRTLSPADPAYIGRYRGD
                     PGQRDTAYHQGTVWPWLIGPFGDAYLKTYGRTKESKKFLMDRLIVPLDRHRFRYAGLG
                     FISEIFDGDPPYRPNGCIAQAWSTAEFIRLLVSLSQIP"
     gene            3164..3832
                     /gene="coaE"
                     /locus_tag="P5573_01430"
     CDS             3164..3832
                     /gene="coaE"
                     /locus_tag="P5573_01430"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HRT26760.1"
                     /translation="MVVRAMLNVGLTGGIASGKSTVDMMFHEKGAYLIDHDVLAHQAE
                     EPDRRAWREIVAFFGPEILNDDRTINRRLLGSIVFKDREKRRILMDIVHPIVFEDWKR
                     RVTDITRKDPSAIIISDIPLLLERGWQDAVDSIILIYVSPEEQIGRLITRNGLSRVEA
                     EERLSSQIPIDEKVLYADFIINNEGPLDETRKEVDRVWVKLLDLEKAKRLKGNHSKLR
                     IKRR"
     gene            3869..4486
                     /locus_tag="P5573_01435"
     CDS             3869..4486
                     /locus_tag="P5573_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HRT26761.1"
                     /translation="MIEKNVVTDFSSRIYEPAVDPSAYVHPLAAVIGNVTVGRNVMVA
                     PGASIRGDEGQPLFIGDDSNVQDGVVIHALETEAHGTPVEKNLHEVDGRKYAVYVGNR
                     VSLAHQAQIHGPAVVLDDTFVGMKVLAFRAYVGKGCVIEPGAILMDGVRVPDGRYVPA
                     GSVIRRQADADKLPAITPDYPLKDLNKGVVHVNVTLAKGYLASGK"
     gene            complement(4574..5533)
                     /locus_tag="P5573_01440"
     CDS             complement(4574..5533)
                     /locus_tag="P5573_01440"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HRT26762.1"
                     /translation="MAGVWVLGENREQLFELLSIGRSLADRYGTTLVAFAFQDAQAAR
                     DCIAHGADRAVVLKYKGQEGPLDVFLPAIVEEVKGKDPDMLLIASTYKLKELAARIAE
                     RLKAGLCSDCIRLVFNEGEKSVEMERLMYGGSAIQRVICLSRPAMATIPPGTFEKAEA
                     GSGRVKEGDVDEIVQTHESAVTILERKVRERESKDIREARTVICVGRGFEKKEDIKLA
                     EELATVLRGEIAGTRPVTEEMHWLPESRCIGLSGIQVRPDLYIGLGVSGQIQHVTGLR
                     DAKVICAVNRDANAPIFGVSDLGIVGDLYDVVPKLVDELKKGL"
     gene            complement(5544..6308)
                     /locus_tag="P5573_01445"
     CDS             complement(5544..6308)
                     /locus_tag="P5573_01445"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HRT26763.1"
                     /translation="MLDIIVCFKWVVDEAFIRRTSSGDLDFSSVDYKISEYDRNAIEA
                     AVRLREECGGSVTAVTVGPPEAAKGVKDALSRGPDRAVFAEDPLFGGLDPSQTAAVLG
                     QVISSKTPFNLIICGEGSGDVYSQQTGPRLAEFLGIPCAAYVQKLTVKEGRVTAERKV
                     EGFIEVDSLPLPALVTVLPDIATPRIPGVKDTLMASKKAVIKVGKNELTAVPPPLLKT
                     KSVRAARVERECEKFTAEVEDIKRFVAALQKRGVLK"
     gene            complement(6396..7535)
                     /locus_tag="P5573_01450"
     CDS             complement(6396..7535)
                     /locus_tag="P5573_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HRT26764.1"
                     /translation="MNFQLTEEQELIRKNIREFAAKYVEPIAAEIDENERHPAELFKK
                     LAEGGWMGIPIPTQYGGSGADYLTNAIVVEEISRSCSSTGFTVSIHVGIACMLILLFG
                     NEEQKKKYLVPLAKGEHMGAFVLTEPGAGTDVMAATTTAAKDGDFYVLNGTKTFISNG
                     PLADTYLVFCWTDKAAGRKGMSSFIIPRGTPGLIPGEHFHKMGLRSSQTSEVVFKDCR
                     VHKDNLLGKEGAGLTNAMTGFDHGRVGIAAQSVGILQAALDESIRYSKERVQMGNPIA
                     RHQAISWMIADMATDLEAARFLTYHAAWLKDQGKPFSKEASMAKLFAAEKAMHHTIKA
                     VQIHGGYGYIKGAKVERLMRDAKITEIYEGTSEAQRMVISGNVLR"
     gene            complement(7592..7876)
                     /locus_tag="P5573_01455"
     CDS             complement(7592..7876)
                     /locus_tag="P5573_01455"
                     /inference="COORDINATES: protein motif:HMM:NF017038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRT26765.1"
                     /translation="MSDESRLKVKLGLDVFKPLKEPHIKIRPGMEKDGRLRPALRICP
                     AGLYTERSDGGISLSIDGCLECGTCRVGCGSDVLDWNYPDGDAGVQYRFG"
     gene            complement(7873..>8707)
                     /locus_tag="P5573_01460"
     CDS             complement(7873..>8707)
                     /locus_tag="P5573_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT26766.1"
                     /translation="ADVVVICDGVLSLLSEKAGLRTAPLPKNYAVGIKEVIKISKEVV
                     DERFGLQGNEGAARLYVGEVTKGRFGGGFIYTNRDSVSLGVVIGIDALLKHEELDAPA
                     AIDSFKERPEVSALIRGGETVEYSAHLIPEGGYDALGKLYGEGVLAAGDAAGFALNIG
                     FTVRGMEYALASGYYAAKAILQAKEDGDFSSRRLSVYKNLLEDSFVLQDLKNFREAPR
                     VIENPRFFGHYPEMIGSIMKDIYGVPEGPKERLYLTVKRRFSLKELWSIFMDLKGVMK
                     I"
BASE COUNT         1965 a         2587 c         2138 g         2017 t
ORIGIN      
        1 ttcccaacca cgcagtcaaa tcccgttcca tatccgccca ggaggtgtaa cgggggacgc
       61 tcaactgggc aacgggttca aattcttcag ccacctcgac cggcgtttga aacctgaaat
      121 ccgggtgctt cagtacctcg cggggaaacg ccctcagaaa atcgaatatt cccatgtcct
      181 cccactgatg ttcgccgaag gtctcataat ccaggaacag attgacgacg tcccccgact
      241 tgtttatctg atgaatccag tgagcatatt tatcggcaac aagcggaaat tccttccact
      301 ttctgtcgga aaacctgaag gcaacgtcat cggacaacct gtaattcttg agcagcagtt
      361 tcagtttcag gcacccgtcc ggccggtaga cgaagtttgg acttctccat ccgaggactt
      421 ggtccgctcc ttcggcaagg atgaccttgt aattcatttt ttctacttcc ctggcaaggt
      481 cgtcgttata gatgagttcc gtattcctga atgtcctagg tgtttggccg aagagtttgc
      541 ggattttcct tttgtgtaac tcgacttggt ctttgaactc tctccgggag aagagaaagg
      601 cgagggagtg atagtatgtc tcgttcagaa actcgacgca ccccgtgtcg gcgagccttt
      661 tgaaaccatc gatcacctcg gggcaatggg tttccatctg ttcgagtacg atgcctgtga
      721 tcgaaaaggc gaccttgaaa tcccctttgt acttccggac aagctccagc atcaaccgat
      781 tggccggaag gtagcacttc tccactactt ttttcaggat tacggcgttc ttttcgtcgt
      841 cttcgtacct atgatcgcgg tcgatatcga agaaggtaaa atgtctgtgc ctgcacggct
      901 gatggacttg gaagtaacaa caaacagacg gcatgtttcc cctcgacaaa cggctctcgt
      961 ccgaccggac gaacctgctc aggcataata tcaaacttta cccgcattgc aagatttaca
     1021 gactcacgga atctgcgata gagacacaag caatctgatg aactcggcgg tactccaggc
     1081 ttgcgcgatg cacccgttgg gacggtacgg cgggtcaccg tcaaaaatct cggaaataaa
     1141 accaagacca gcataacgga aacggtgcct atcgaggggt acgataagcc ggtccatcag
     1201 aaacttctta gactcttttg tgcggccata tgtcttcagg taagcatcac cgaagggtcc
     1261 gatgagccac ggccacacgg ttccttggtg gtaagccgtg tcccgctgtc cagggtcgcc
     1321 cctgtacctc ccgatgtagg ctgggtcggc gggagaaagt gttcgcaacc catagggcgt
     1381 caacagatca ctgaccacct ttttgagcac gccctcacac ttatcggcat cgattgccct
     1441 ataagggagc gagacggcga tgatttgatt cggcctgacg gaccggtcta tcccatcccc
     1501 agtaaatacg tcggcaagat aggacccgtc tgtcagccaa aagacttttt caaaagaatc
     1561 ccttatgttg gaaatggtat ccttcaagtc catatcgccg tcctgaaact tatccgacag
     1621 gtctttcgca atacataggg cgttgtacca gagggcattg acctcgacgg cataccccgc
     1681 ccgcggtgtc acaggccttc catccacaaa agcatccatc caagtcagtt gcgtcgtaga
     1741 gtcgcccgcg tgcagcagcc cgtttccggc catgtaaatt tcatggtccg tccctgccat
     1801 aaagctccgg atgatcttct tcatgacggg ccagaatgat tcttctatgg ttttcaagtc
     1861 acccgtatgg tccaacatct gctgaacggc ccagaaatac cagagagaac tatccacgga
     1921 attgtacgcg gctttgccgt cgtccgtgaa gaaattaggc aaaagaccgc ccctctcatg
     1981 ctcaccgatc gccttcagga tttccactcc atcccgcggc ctccccgagc aaaaagtcag
     2041 gcctggcaat gaaatcaaag tgtccctgcc ccagtcagaa aaccagtggt aacccgcaat
     2101 gaccgtattc cgaccggtcg gcgtcctgac agggaaacac cgaccggact gaatcgcgcc
     2161 ctccaacatc tttcgctccc catcggagcg agctgcctgc acacagcttc ttactataga
     2221 agacacctgt ttgcggcgat tctcttcgtt tttccatatc gtctgaggat ccgtctcgca
     2281 ccctcggacg gaggccgaga gataaacttc tcggccttgc gccaactcca tctcaagcag
     2341 gccgggcata aacagatctt cacgaaaatc ataccccctc tcctgctccc ggacgtactc
     2401 gaagtcttta taccaaaggg gcagaggcgt aaattcggac tctccgtccg tccgaatgac
     2461 caagcccggc atcccttcgt acggctgaag ctcgaatccg tacgccgttc gcgaaacatc
     2521 ccgtcttatg aaggagtttt ctctggacaa tcgatggtgc ccccggaaag ccagaaatgg
     2581 tttcagtctc aggcggaggg tcccgccgga ttcgcaggaa tagcgaacca aaacacactt
     2641 ccctccttga acaagcatga tccgccggtg gatgacgata ccgccggctt taagcaggaa
     2701 acgaggacag tagtcgaatt cgtacccttg cagggcgtgg ttgctgccgg ggaagaaaac
     2761 acctggatat tggtgacagg aaagaaagac ttcttcgttt ttcacaaaaa cagaatcttc
     2821 gaacttcgaa aggaggacat gtctgccggg cgggtcggca aggttaggta caaagagtcc
     2881 atgatatttt ctcgtatggc acatcaggat ggtgctcgag gcataacccc cggcgccgtc
     2941 cgcttcgagc cattcaagac tcaaggactt gccgatgttt ctgcagatgt cttctcccac
     3001 caggaactgc atggcagccg ccttcccagg atattatagc tcgcctgagt ttccccggtt
     3061 ttcgatattc cctccttccg aatgggaaac ttgcgaaaac tatgaccgta aacagggacg
     3121 gtgtcaaggc aattgtcttt ttcattgatg acctatgagc cttatggtag taagagccat
     3181 gttgaacgtt ggattgacag gtggtattgc gagcgggaaa tcgaccgtcg atatgatgtt
     3241 ccatgaaaaa ggcgcctatc tcatcgacca tgatgtcctt gcccaccaag ccgaagaacc
     3301 ggaccgtcgt gcctggcgag agatcgtcgc cttcttcggc cctgaaatcc tcaacgacga
     3361 ccgaacgatc aaccggcgcc tgctgggaag catcgtcttc aaagacaggg aaaaacgccg
     3421 tatcctgatg gacatcgtgc acccgatcgt ttttgaggat tggaagcgcc gggtcaccga
     3481 catcacgcga aaggacccgt cggccatcat catatccgac atcccattgc ttctcgaaag
     3541 aggctggcag gacgccgtgg attcgatcat tctcatttac gtatccccgg aggagcagat
     3601 agggagattg atcacacgca acggcctgag cagggtcgag gcggaggaaa ggctcagttc
     3661 ccagatcccc atagacgaaa aggtcctcta cgccgatttt atcatcaaca atgaggggcc
     3721 gctcgacgag accagaaaag aagtggatag ggtatgggtc aagttgctgg atcttgaaaa
     3781 agccaaaaga ctgaaaggca accacagcaa gcttagaata aaaagaaggt gactgccaaa
     3841 aaggcaaagt tcatcgggag gaaagataat gatcgaaaaa aatgtggtga cggatttcag
     3901 ttcccgcatt tatgaacctg cggtcgaccc atcagcatat gtgcatccgc tcgcagcggt
     3961 catcggcaac gtgactgtcg gcagaaacgt gatggtggcc cctggggctt ccatccgcgg
     4021 cgatgaaggg cagccccttt tcatcggtga tgactctaac gtccaggacg gggttgtcat
     4081 ccatgccctt gagaccgagg ctcacggcac gcccgtcgaa aaaaaccttc atgaagtcga
     4141 cggcagaaag tacgccgttt atgtaggaaa cagggtatct ctcgcccacc aggcacagat
     4201 ccacggcccc gccgtagtac ttgacgatac ctttgtgggt atgaaggtcc tcgcgttccg
     4261 ggcctatgtg ggaaagggct gtgtcatcga acccggtgcc atcctgatgg acggagtcag
     4321 ggtgcccgac ggccgttacg tccctgccgg atcggtcatc cgcagacagg cggacgcgga
     4381 caaacttccg gctatcaccc ccgactaccc cctgaaagac ctcaataaag gcgtcgtgca
     4441 cgtcaacgta accctcgcaa agggatatct tgcatcagga aagtgacctt ttaaacgttg
     4501 tgatacagaa cagggcgccg cggcctgcta cagccggcgc cctgttgttg gctgaccgtt
     4561 gtgggtcgac cggttagagc cctttcttca attcatctac cagtttcggc acgacgtcgt
     4621 agagatcgcc gactataccg aggtccgaaa ccccgaagat cggcgcattc gcatcgcggt
     4681 tgacggcaca gatgactttc gcgtcccgca aacccgtcac atgctgtatc tggccggaaa
     4741 caccgaggcc gatgtagagg tcgggtctca cctggatgcc ggataaaccg atgcagcggc
     4801 tctcaggcag ccagtgcatt tcttctgtaa ccggccttgt tcctgctatt tctccccgaa
     4861 ggaccgtcgc gagttcttcg gccagcttta tatcttcctt cttctcgaag cccctcccga
     4921 cacatatcac ggtccgcgct tctcttatgt cttttgattc cctctctctg acttttcttt
     4981 cgagaatggt aacggccgat tcgtgagttt gaacgatctc gtctacatcc ccttctttta
     5041 cgcgtcccga gccggcttcg gctttttcga aggtgccggg cgggatggtc gccatcgccg
     5101 gtcttgacag acagatcacc ctttgtatgg ccgacccgcc gtacatcaat cgctccattt
     5161 cgaccgactt ctctccttcg ttgaagacga gcctgataca gtcactgcat agaccggctt
     5221 tcagcctctc tgcgatacgt gctgccagtt ctttcaactt gtaagttgac gctatgagga
     5281 gcatgtccgg gtctttgccc ttaacctcct ccacaatcgc cggcaggaac acatccaaag
     5341 gaccttcttg ccctttatat ttcaggacga ccgcccggtc tgctccgtgc gcaatacagt
     5401 cccgggctgc ctgcgcgtct tgaaaagcaa aggccacgag agtagtccca tatctatcgg
     5461 ccaatgacct cccgatactc agaagttcga aaagctgctc ccgattttcc cccaaaaccc
     5521 atacgcccgc catttcccgc tccttatttt aagacgcccc ttttctgcaa ggccgccaca
     5581 aaccgcttaa tatcttcgac ttccgccgtg aatttttcgc actcccgctc cacccgtgcg
     5641 gccctgaccg acttcgtttt cagcagggga ggaggaacgg ccgtcagttc attcttcccc
     5701 acctttatca cggccttctt cgacgccatg agcgtatcct taacccccgg gatccgcggc
     5761 gtcgcgatgt cgggcaacac agtaaccagc gccggtaagg gcagggagtc gacttcgatg
     5821 aacccttcga ccttccgttc cgccgtcacc cttccttctt taaccgtcaa cttctggaca
     5881 taggccgcac aagggatgcc gagaaattcg gcgagacgag ggcccgtttg ttgcgaatag
     5941 acatcacccg agccctcacc gcagataatc agattgaacg gtgttttcga actgatgacc
     6001 tgccccagga ccgcagcagt ctgagacggg tcaagccccc caaaaagcgg gtcttcggca
     6061 aataccgccc ggtcagggcc gcgcgaaagg gcgtccttga ctcctttcgc cgcctccggt
     6121 ggaccgacgg tcaccgccgt gacactgcca ccgcattctt ccctcagccg aacggcggcc
     6181 tcgatcgcgt tcctgtcata ttcgcttatc ttgtaatcga cggaagaaaa atcgagatcg
     6241 ccggatgatg ttcttctgat gaatgcctca tcaacgaccc acttgaaaca aacaatgatg
     6301 tcgagcatgg gaatcccctg tttctgatat ggaccgaggg cagaaggccg ttgcaaattt
     6361 acctacagac ttctgccccg ttcctttcac aatttttatc tgagtacatt ccccgaaatg
     6421 accatgcgct gggcctcgga agttccttcg taaatctcag tgatcttggc gtcccgcatc
     6481 agtcgttcca ccttcgcccc cttgatgtag ccataaccgc cgtgaatctg cacggcctta
     6541 atcgtatggt gcatggcctt ctcggcggcg aagagcttcg ccatcgaggc ttccttgctg
     6601 aacggtttcc cctgatcctt gagccatgcg gcatggtaag tcagaaaccg cgctgcctca
     6661 aggtccgtcg ccatgtcggc gatcatccaa gagatagcct gatgtcgggc aatcgggttc
     6721 cccatctgga cacgttcttt cgagtacctg attgactcat cgagggcggc ctgtaaaatt
     6781 cctacagact gggcggctat ccccacgcga ccatggtcga aaccggtcat ggcattcgtc
     6841 aaacccgccc cctctttgcc gagcagattg tctttatgga cgcggcaatc cttgaacacg
     6901 acctccgatg tctgggagga ccggagcccc atcttgtgga aatgctctcc gggaatcagt
     6961 ccgggtgtgc ctctgggaat tatgaacgag ctcatcccct tccgtcccgc cgccttatcg
     7021 gtccagcaga aaacgaggta tgtatcggcc aggggcccgt tggagatgaa tgtcttcgtt
     7081 ccgttcagaa cgtagaagtc accgtccttc gcggcggtcg tcgtagcggc catcacatcc
     7141 gttcccgccc cgggctccgt cagaacaaag gcgcccatat gctcaccctt cgccaggggt
     7201 acgagatatt ttttcttctg ctcttcgttc ccgaacagca ggatcagcat gcaggcaata
     7261 ccgacatgga tggaaacggt gaaaccggtg gacgaacagg accgcgaaat ctcttcgaca
     7321 actattgcgt tggtcagata gtccgccccg gaaccaccgt attgcgtcgg gatcgggatg
     7381 cccatccacc ccccttccgc caactttttg aacaattcag ccggatgccg ttcgttttcg
     7441 tcgatttcag ccgcgatagg ttccacgtac ttggccgcaa actccctgat gttcttccga
     7501 atcaattcct gctcttccgt caactgaaaa ttcatcttac ccctccttct gttgatctat
     7561 ttctcgacct tttcgcccgt tcgctgtcgg atcacccgaa gcggtactgg acgccggcat
     7621 cgccgtccgg ataattccag tcgagcacgt cgctgccgca tcctaccctg cacgtcccgc
     7681 attcaaggca cccgtctatg gaaagcgaga tgccgccgtc cgacctctcc gtgtacagcc
     7741 cggcaggaca gatcctgagt gcgggcctca gacgaccatc cttctccatg ccgggtctta
     7801 ttttgatatg gggttcttta aggggcttaa aaacatccag ccccaacttc accttcaatc
     7861 tcgattcgtc gctcatatct tcatcactcc tttcaaatcc atgaagatcg accacaattc
     7921 tttgagactg aagcgtctct tcaccgtgag gtaaagcctt tccttcggtc cctccggaac
     7981 cccgtaaata tccttcatta tgcttcctat catctcggga tagtgcccga aaaaacgcgg
     8041 gttctctatc acccgcgggg cttcacggaa atttttcagg tcctgtaaga caaaactgtc
     8101 ttcaaggaga ttcttgtaga cagaaagtct gcgggagctg aagtctccat cttctttggc
     8161 ctgtagaatc gccttggcgg cgtagtaccc cgaagcaagc gcgtattcca tgccccttac
     8221 tgtaaagcca atattcagcg cgaacccggc agcgtcgccg gcagcaagaa ctccctcacc
     8281 gtaaagcttg ccgagggcat cgtagccccc ttccgggatg aggtgggccg aatactcgac
     8341 cgtttcccct cctctgatga gtgcggaaac ctccggcctt tccttgaatg aatcgatcgc
     8401 cgcgggcgca tcgagttcct catgtttcag cagggcatcg atcccgataa cgaccccgag
     8461 actgacactg tcccgattgg tatagataaa cccgccaccg aatcttccct tcgtcacttc
     8521 gccgacataa agacgggcgg ccccttcatt gccttggagg ccgaaccgtt catcgaccac
     8581 ttccttgctt atcttgatga cctcttttat tcccaccgca taatttttcg gcaacggcgc
     8641 cgttctaaga cccgctttct ccgacagaag agacaggact ccatcgcaaa tcaccaccac
     8701 gtcggcg
//
LOCUS       DAOTMX010000027         8662 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_7368_length_8662_cov_3.292553, whole genome shotgun sequence.
ACCESSION   DAOTMX010000027 DAOTMX010000000
VERSION     DAOTMX010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 8662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8662
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7368_length_8662_cov_3.292553"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="P5573_01465"
     CDS             <1..497
                     /locus_tag="P5573_01465"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HRT26767.1"
                     /translation="GGDPDTRHTEQERSAELLTARELIWGGYRDTQLTENMNRLIHYI
                     EEVLKRVKPEFTFVNFGDDTHQDHRALSRATVSATRYIKNVIFYEVPTTQNFSPTVFV
                     DINETFRDKISLLLMHNSQVMKTNIEGLSIKDVVQSTAIFRGIQGRVRYAEGFVPLRL
                     FINV"
     gene            503..1552
                     /locus_tag="P5573_01470"
     CDS             503..1552
                     /locus_tag="P5573_01470"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRT26768.1"
                     /translation="MLSMIPHSRPSVDAQDRAALLSVLESGHLSQGKEVARLEDELAS
                     FIGSGTGVAVSSGTAALHLSLIALEVGSGDEVILPSYVCPALLNAVHYVSATPVLADI
                     DPKTFNMDVQSVRHRITAKTKAVILPHMFGLAADVDDFISLGIPIIEDCALSVGGDYK
                     GQHLGSFGVLSVFSFYATKMLAAGEGGMVLCNDEALAGAVRDLRDYDEKADYRVRYNY
                     KMTDLQAALVISQLKRLPFFIERRREIADRYRRLLETVNLSPPFVPEECGHVFYRYIV
                     LMDRADEFLAWMYRRGVECRRPVFRPLHHYLGLAGYPCTGEVHRRAVSIPTYPSLTDT
                     ETMEITQEIKEFCRR"
     gene            1549..2706
                     /locus_tag="P5573_01475"
     CDS             1549..2706
                     /locus_tag="P5573_01475"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HRT26769.1"
                     /translation="MKIAPVTVERSKISPKRILWLLVGIFFLTLLTPPVRLYFFEMSK
                     RWLYVLSYAGSLAFLLTPIMRAVAIRFGVLDFPAGRKIHTTATPLLGGVAVIVAFTTA
                     LIANSILEQRIVVFLGGSLIVAVVSLIDDWRGLSASVKLLFQVVVVLALIYNDIILDL
                     FPFKTTWGHCLNVLLTLVWVVGITNAMNFMDGMDGLAAGLSAVIAFFIGLVAFQTNQP
                     FMGWIAAAVLGSCLGFLPFNFRVRKPAAIFLGDAGSTFLGFVLACLAVIGDWDEDPVV
                     SFSAPVLIFWVLIYDMAHITVERILKGKVRSLKEWIDYVGKDHLHHRICGLIGDRRKT
                     VLLIYSLSATFGLSAIALKNARTIDSIVLVIQALLITLIVSILEYSGRNRD"
     gene            complement(2726..4795)
                     /gene="glyS"
                     /locus_tag="P5573_01480"
                     /pseudo
     CDS             complement(2726..4795)
                     /gene="glyS"
                     /locus_tag="P5573_01480"
                     /inference="COORDINATES: protein motif:HMM:NF014183.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
     gene            complement(4857..5741)
                     /gene="glyQ"
                     /locus_tag="P5573_01485"
     CDS             complement(4857..5741)
                     /gene="glyQ"
                     /locus_tag="P5573_01485"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014896110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HRT26770.1"
                     /translation="MTFQELVFSLERYWAAQGCIVQQPYDLEVGAGTFNPATFLRSLG
                     PEPWNVAYVEPSRRPTDGRYGENPNRLQHYYQYQVVMKPSPMDIQDMYLESLKSFGID
                     PLEHDIRFVKDDWESPTVGAWGLGWEVWLDGMEITQFTYFQQVGGVDLDPICVEITYG
                     IERIAMYLQGIDNVYDLEWGHGIKYGDVHHQGEVEWSVYNFEEADVEMLRRIFDMYEA
                     EGVRLAARQLVLPTYDCCLKCSHLFNLLDARGAISVAERTSYIGRVRNLARLSAEGYL
                     RQRENLGYPLMGKWSKKV"
     gene            complement(5817..6596)
                     /gene="recO"
                     /locus_tag="P5573_01490"
     CDS             complement(5817..6596)
                     /gene="recO"
                     /locus_tag="P5573_01490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HRT26771.1"
                     /translation="MTSGIKTSKYTAAAVVLRSLDYGDSDRIVTFYTEEFGKVKGIAK
                     GARRSRRRFVNALEDFSLASLVFSKKRPEGLALIEACDVQNHYPGIRADLRKALTAAY
                     LAELVDRFTIEEKKNDGLFLLLVEFFDLLEKEGFAEGVVRIFELRLLKLSGYDPVLDR
                     CVGCNIPLKDMSSIYFDPAAGGIRCGCAPRAVDVYPLTTGTVKLLLLGKTMDVGKLPR
                     LLFSERSLEESRRALAPFIQRLVGKELKSLKVLNEVERLAL"
     gene            complement(6610..7917)
                     /locus_tag="P5573_01495"
     CDS             complement(6610..7917)
                     /locus_tag="P5573_01495"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HRT26772.1"
                     /translation="MSSLLLKGARIVDPSQKLDGNRDLLVENGKIAAIGTDIGPGHAG
                     SSGEKGSGTKILDVGGKVVVPGLIDMHTHLREPGYEYKETIRTGSRAAVMGGFTAIAC
                     MPNTEPVNDNRSVTEFILRRAKECDLARVYPVAAISKGSAGHILAELGDLRDAGAVAY
                     SDDGNPVMNSRLMRKALEYAASLGVVVISHCEDLTISKGGLMNESFVSTELGLRGMPA
                     VAEDIMVARDIALAEYTGTPVHIAHVSTAGAVRLVRDAKERGVKVTAETAPHYFTLTE
                     EALRSYDTNAKVNPPLRGFRDVEAVKAGLRDGTIDVIASDHAPQSIIEKDVEFENAAN
                     GMVGLETSLGLSLRLVEENLLSLSDLVMKMSVNPAAILRLSGGSLRVGADADMTVIDL
                     AQVWVVDVGTFRSKSRNSPFHGRTLKGKAFCTIVGGEIKHIEG"
     gene            complement(7880..>8662)
                     /locus_tag="P5573_01500"
     CDS             complement(7880..>8662)
                     /locus_tag="P5573_01500"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041585001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HRT26773.1"
                     /translation="TSFEIAAKRLSADTVNISSTTSSVVKGETIIDTARNLEAMNPDI
                     IVVRHNCSGAPRLLAGLVKQSVINAGDGAHEHPTQALLDIMTVRQKKKRVEGLKVVIV
                     GDIAHSRVARSNIYGLTKMGARVTVSGPSVMMPRDVDKMGVDVQYDMKEALRGADIVM
                     MLRIQTERQEMSLFPSLREYANYYCLNREKLRYAKDDVIVMHPGPVNRGVEISSDIAD
                     GPFSVILNQVTNGVAVRMALLYLLAGSEVRNEQSAVEGGKNR"
BASE COUNT         1804 a         2643 c         2194 g         2021 t
ORIGIN      
        1 tggggggcga tcccgacacg cgccacaccg agcaggaaag atccgctgag ctcctcacgg
       61 ccagggaact gatatggggc ggttaccgcg atacccagtt gacggagaat atgaaccgcc
      121 tcatccatta catagaggaa gtgctgaaaa gggtcaagcc ggaattcacc ttcgtcaatt
      181 tcggggacga cacgcatcaa gaccatcggg ccttgtccag ggccaccgtg tcggcaacac
      241 ggtacataaa aaacgtcatt ttctatgaag ttccgacgac gcagaatttc tcccccaccg
      301 ttttcgtgga cataaatgaa accttccgcg acaagatttc acttctgctg atgcacaatt
      361 cacaagttat gaagaccaac atcgaggggc tctccataaa agacgtcgtg cagtcgacgg
      421 caatcttccg cggaatccaa gggcgggttc gttacgccga gggatttgta ccgctgcgct
      481 tgttcataaa cgtttgacga ggatgctatc gatgattccc cattcccgcc cctcggtcga
      541 cgctcaggat agggcggcct tgctttccgt cctcgaatcg ggacatctat cccaggggaa
      601 agaagtggca cgcctcgaag acgagcttgc ttcctttatc gggtcgggaa cgggcgtggc
      661 cgtcagctcg gggacggcgg cgcttcacct ctcgctcatc gcgctggaag tagggagcgg
      721 tgacgaggtc atccttccga gctatgtctg cccggccctc ctgaacgccg ttcattatgt
      781 gtccgcaacg cctgtcctcg cagacatcga cccgaagaca ttcaacatgg atgtgcagtc
      841 tgtgcgacac agaataacag caaagacgaa ggccgtcatc ctacctcaca tgttcggcct
      901 cgctgcagat gtagacgatt tcatttccct cggaataccc atcatcgaag attgtgccct
      961 ttcggttgga ggggattata aaggacaaca ccttggctct ttcggtgtct tatccgtatt
     1021 ctccttctac gccacaaaaa tgctcgccgc cggagaaggg ggcatggtcc tttgcaatga
     1081 cgaggccctg gccggggccg tccgtgacct gagggactac gacgagaaag cggactaccg
     1141 ggtccggtac aactacaaga tgacggacct tcaggccgcc ctggtaatca gccagttgaa
     1201 aaggctcccc ttcttcatcg agaggcgcag ggaaatcgcc gaccgttacc gtcgtctcct
     1261 cgaaacggtc aatctctccc caccctttgt gcccgaggaa tgcgggcacg tcttttaccg
     1321 ctacatcgtg ctcatggatc gggctgatga atttctcgca tggatgtatc gccggggggt
     1381 cgaatgccga aggccggtgt tccgtcccct ccaccactac ctcggtctcg caggctatcc
     1441 ctgcacggga gaagtccacc gccgggcagt ctcgataccc acctacccgt ctctcacaga
     1501 caccgagacg atggaaatta cacaggaaat caaggaattt tgtcgacgat gaaaatcgcc
     1561 cctgtgactg tcgagagatc aaaaataagc cccaaacgca tactgtggct tctcgtcggt
     1621 atattctttt tgacgctgtt gacgccgccg gtgcgtctct atttttttga gatgagcaag
     1681 agatggctct atgtcttgag ctacgccggc tctcttgcct ttctgctgac ccccatcatg
     1741 agggcggtgg ccatcagatt cggcgtctta gattttccgg cgggaagaaa aatccatacg
     1801 acggcgacgc ctctccttgg aggtgtggcc gtcatcgtcg cctttacaac ggccctcatc
     1861 gccaattcga tcctggagca gaggatagtc gtgtttctcg gcggcagtct gattgtcgcc
     1921 gtagtcagcc ttatcgacga ctggcgagga ctctcggcat cggtcaaact cctgttccag
     1981 gtcgtcgtcg tccttgctct catctataac gacatcatcc tcgacctctt ccccttcaag
     2041 accacatggg gacactgctt gaacgtcctc ttgaccctgg tctgggtcgt cggaatcacc
     2101 aacgccatga atttcatgga cgggatggac ggtcttgccg ccggcctgag tgccgtcatc
     2161 gcctttttta tcggcctcgt cgccttccag acaaatcaac ccttcatggg gtggatcgcc
     2221 gccgccgtcc tgggaagctg cctcggtttt cttcccttca acttccgggt tcgaaaaccg
     2281 gcggccatct tcctgggcga cgcagggagc acattcctgg gattcgttct tgcgtgcctt
     2341 gccgtgatcg gcgactggga tgaagaccct gtcgtgtcat tctcggcacc ggtcctcatc
     2401 ttctgggtat tgatttacga catggcacac atcacagtgg agagaatact gaaaggaaag
     2461 gtgaggtctc tcaaagaatg gatcgattac gtagggaaag accacttgca tcacagaatc
     2521 tgcggcctca tcggagacag gagaaaaacc gtcctcctga tctattcgtt gagcgcaacc
     2581 ttcggcctca gcgccatcgc gctcaagaac gcccgcacca tagacagcat cgtcctcgtc
     2641 atacaggccc tcctgatcac cctgatcgtc tcgatcttgg aatactccgg ccgcaaccgc
     2701 gactgacctt tttcaatcaa gaatatcagc ctgttacgat ttttgagaag tccgctatcc
     2761 ggtaaaagag tttcgatatg gcctcgagca gggaaagccg gttaaaccgc accgcttcgt
     2821 cttccgccat gacgagcacc gcctcgaaga actcgtctac aaatttcctc atgcccgcga
     2881 gctcccggag cgcctcctcg tactcgtcct tatcgatgaa ggtccctgcc ctatctttgg
     2941 tctccaggta ggcctgatag agggcctctt cctctctttg cgtaaacagg gaagcgtcta
     3001 ctctcccgtt ctcgaaaccc ttgatgatgt tgcatacccg cttgaaggcg atgccgaggg
     3061 gttcaaagtc cggcctcttc ttgaaatctt ccatggccat taccttcttc acggtcttca
     3121 ccaagtcatg gaagcccgcc gacagcacgg catcgacgac atcgtatgaa tatccctgcg
     3181 ttaatagttg attttccagt ctgcctttga agaactcaag gacgtcccgt ttgacttcct
     3241 cacggggcct cttcaaccgg tcggcgagaa tggcgaggct ttcatccacc aggttgtcga
     3301 gagaaagcgg atatcccttg ttcagtatga tgttgatcac cccgagcgcc tgcctccgta
     3361 gtgcataagg gtccgccgct cccgtcggta tctggttcac cccgaaaaac ccgcagatgg
     3421 tatctacctt gtccgcgatg ctgacgatcg acccctcatc gctctgcggc agttttcccc
     3481 cggccgaggt cggcaggtaa tgttcgtaaa tggcctgggc cacgactggg ttctctccgg
     3541 ccaacaatgc gtattcccgc cccatgaccc cctggaggtc ggggaattca tagaccatcc
     3601 tggtctcaag gtccgccttc gcgagatggg ccgcgcggtc gacatgctct ttgagccccg
     3661 ggtcgacctt ctcggctatc aaaccggcga gtttcctgaa ccgaccgact ttttcgtaag
     3721 acgttcccaa tagggaatgg aagacgactt ttttaagttt ctcgaaatgc tcctcaagcg
     3781 gtatcttccg gtcttccctg aagaagaatt tcgcgtcgga aagacgggcc cgcaacacct
     3841 tctcgtttcc tttgatgacg acttcggggt tacgcaccag tgtattattc acggcgatga
     3901 aatgaggaag gagcttgtcc ttgtcgtcga cgaccgggaa gtacttctga tgcccgacca
     3961 tggaagtaat caggacatcc tccgggagct caaggaattc ggcgtcgaaa ctcccgcaga
     4021 ccgccgaggg atattcgaca agatagttga cctcttccaa gagcacgtca tgcctgagca
     4081 cacggccgga aacagtcttt gccgctttgt ctgtcgccgc cacgatgatc gccctccgct
     4141 cttcggggtc gacaacgaca taacggtcaa gggcctgcct catgtaatcg gaaaaaccct
     4201 caacctcaaa cggcccgggg ttcatgaacc ggtgaccgta gctcttgttt ccgctggaaa
     4261 tgtttcccag cttgaacgcg acaaccttac cgccatagag cgccaggatc caatgaatag
     4321 gcctcgcaaa acgtatgtct aagttcatcc agcgcatgga cttctggaag ggtatggaca
     4381 ggataaaccg cttcaggata tccggcaggc tcgcggcggt ctcttcaccc ttgattacct
     4441 tcctgacgca gatgtactct cccttttccg taacgactgt tccaacttcc gagatgtcga
     4501 tcccctgact cttcgcaaac cctagggccg ccttggtcgg gtttccttcg tcgtcgtagg
     4561 agaccctctt cgcggggccg agtttctcaa cgacctggtc ttcttgttgc tcggccacgt
     4621 cttctacacc aagaacgagt cacctggggg tcgcaagggt cttgaccacg ccgtgacgaa
     4681 tacgcgcctg ggcaaattcc cggccgatga tttcttccat gtctttcagt gccttgggta
     4741 aaaaatcggc gggtatttcc tcggtcccga tttccagtaa cagctctttc cccatcaatt
     4801 atctccgttg aaaactcatt ccaaatggca ccacatatgc cgataggcct cgaattctac
     4861 acttttttcg accatttccc catcagggga taacccagat tctctctctg cctcaggtag
     4921 ccttccgcgc tcagccttgc caggtttcgg acccttccga tgtagctcgt cctctcggcc
     4981 acactgatcg ccccgcgggc gtcgaggaga ttgaagagat gggaacactt caggcagcag
     5041 tcgtaggtcg gcaggaccag ttgccttgcc gcaaggcgta caccctcggc ctcatacatg
     5101 tcgaatattc gcctcagcat ctcgacatca gcctcttcga agttgtagac cgaccattcc
     5161 acctctccct gatggtggac gtcgccgtat ttgatcccgt gtccccactc caggtcatag
     5221 acattgtcga taccctgaag gtacatggcg atacgctcga tgccgtaagt gatttcaacg
     5281 cagatggggt cgaggtccac accgccgacc tgttgaaaat aggtaaactg cgtgatttcc
     5341 atcccgtcaa gccatacttc ccatcccaaa ccccaggccc cgaccgtcgg tgattcccag
     5401 tcatctttca caaaacggat gtcatgctca agggggtcga tcccgaaact cttcagcgat
     5461 tcgagataca tgtcctggat atccatcggt gaaggtttca taacgacctg atactgatag
     5521 tagtgctgga gcctattcgg gttttcgccg taacgtccgt cggtcgggcg gcgtgacggc
     5581 tcgacatagg ccacgttcca cggctccggc ccgagggacc gcaggaaggt cgccggattg
     5641 aatgtccccg cgccgacttc caggtcgtaa ggttgttgga cgatacaacc ctgggcggcc
     5701 cagtaccttt cgagggaaaa aaccagttcc tggaaagtca tctatcctcc taaaactcag
     5761 gcgttcagtt acaagggcgg ggcatagcac taccctttat ctcctgtcaa gggaaatcag
     5821 agcgccagcc tttcgacctc attgagaacc ttcagtgatt tcagctcttt gccgacgaga
     5881 cgttgtatga atggagccaa agcccggcga ctttcctcca gggatctttc cgaaaataga
     5941 agtcgcggaa gtttgccgac gtccatcgtt ttacccaaga gaagcagttt gacggtcccg
     6001 gtcgtcaggg gataaacgtc taccgcacgg ggagcgcaac cgcatcggat cccccccgca
     6061 gccgggtcaa aatatatgct cgacatgtcc ttcagcggga tgttgcatcc cacgcaccgg
     6121 tcgagcacag ggtcataacc agaaagtttc aggaggcgga gttcaaaaat ccggacaacg
     6181 ccctcggcga agccttcttt ttccagcaag tcgaaaaact cgaccaggag gagaaaaagg
     6241 ccgtcgtttt tcttctcctc gatggtaaac cggtcgacga gctcggcgag ataggcggcc
     6301 gtaagtgcct ttcgcaggtc cgccctgatt cccgggtagt ggttttggac gtcacaggcc
     6361 tctatcagcg caaggccctc aggccgtttt ttcgaaaaca cgagggatgc cagggaaaaa
     6421 tcttcgagcg cgttgacaaa ccgtcttctg ctccttcttg cgcccttggc gatccctttg
     6481 accttaccga actcttcagt atagaaggtt acgatcctat ccgaatcccc atagtcgagc
     6541 gacctgagga cgaccgccgc cgctgtatat ttgctggttt ttatacctga agtcatcttc
     6601 ttgacaggat catccttcga tatgcttaat ctctccccct acgatcgtac aaaacgcctt
     6661 tcccttcaac gtccggccgt gaaaaggcga gttacgactt ttcgacctga aggttccgac
     6721 atctacgacc catacctgcg caaggtcgat gacggtcatg tctgcgtctg cgcccacccg
     6781 cagcgaaccc ccggacaacc tcaggatggc agcggggttc acgctcatct tcatcaccag
     6841 gtccgaaagg gacaagaggt tctcctcgac cagtctcagg ctcaacccca gagacgtctc
     6901 caatcccacc atgccgttgg ccgcattctc gaattccacg tccttctcga tgatcgactg
     6961 gggcgcgtgg tcgctcgcta tgacgtcgat ggtcccatcc ctcagtccag ctttgacggc
     7021 ttccacgtca cgaaaccccc tgagcggggg gttgactttc gcgttcgtgt catagctcct
     7081 caacgcctcc tccgtaaggg taaaatagtg gggcgccgtc tccgccgtca cctttacgcc
     7141 ccgctccttg gcgtccctga ccaggcgaac ggccccggcc gtgctcacgt gggcgatgtg
     7201 aacaggcgtc cccgtgtatt cggcaagggc gatgtcccgg gcaaccatga tgtcttccgc
     7261 cacggccggc atccctctaa gtccgagctc tgtcgacacg aagctctcgt tcatcagccc
     7321 acccttcgag atggtcaggt cttcacagtg agaaatgacg acgacgccga gcgatgccgc
     7381 gtattcgagg gccttcctca tcaggcgact gttcatcacg gggtttccgt cgtccgaata
     7441 ggcgaccgcg ccggcgtccc gaaggtcgcc caattcggca agaatatgcc cagcggaacc
     7501 tttgctgatc gcggcaacgg ggtaaacccg ggccaagtcg cattctttcg ccctcctcaa
     7561 gatgaactcc gtcaccgaac ggttgtcgtt caccggctcc gtgttgggca tacaggcgat
     7621 cgccgtaaaa ccacccatta ccgccgcacg gctccccgtc cggatggtct ccttgtattc
     7681 gtaaccgggc tcacgtaagt gggtgtgcat atctatcagg cccggcacaa cgaccttgcc
     7741 gcccacgtcc aaaatcttcg tcccgctccc cttttccccg ctacttcccg cgtgccccgg
     7801 cccgatatcc gttccgattg ccgctatttt tccgttctca accaacagat cgcgattccc
     7861 gtccagcttc tgggaagggt caacgattct tgcccccttc aacagcagac tgctcattcc
     7921 taacctcact tccggcaagc agataaagaa gcgccatccg gacggcgacc ccgttcgtca
     7981 cctggtttag gatgacggag aacggtccgt cggcgatatc agatgaaatc tcgacgccgc
     8041 ggttgaccgg gcccgggtgc atgacgataa cgtcgtcttt ggcatacctc agtttttccc
     8101 tgttcaagca ataataattc gcatactccc gtaacgacgg gaacaggctc atctcctgcc
     8161 tttccgtctg gatcctcagc atcatgacga tgtcggcccc cctcaaggcc tccttcatgt
     8221 cgtattgaac gtccactccc atcttgtcga cgtcccgcgg catcatgacg gacgggcccg
     8281 atacggttac cctggcgccc attttcgtca gaccgtatat gttggaccgc gcgaccctgc
     8341 tgtgagcgat gtcaccgacg attaccactt tcagtccttc gacccttttc tttttctggc
     8401 ggactgtcat gatgtccaat agcgcctggg tagggtgttc atgcgccccg tctccggcgt
     8461 ttatcacgga ttgcttgacc agtccggcaa gcaaccgtgg tgcccctgaa cagttgtggc
     8521 ggacaactat tatgtccggg ttcatggcct ccaggttcct cgccgtatcg atgatcgttt
     8581 caccttttac gacactgctc gttgtcgagg agatatttac agtgtccgca ctcagtcgct
     8641 tcgccgcgat ctcaaaggat gt
//
LOCUS       DAOTMX010000028         8434 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_7579_length_8434_cov_4.076501, whole genome shotgun sequence.
ACCESSION   DAOTMX010000028 DAOTMX010000000
VERSION     DAOTMX010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 8434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8434
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7579_length_8434_cov_4.076501"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..247)
                     /locus_tag="P5573_01505"
                     /pseudo
     CDS             complement(98..247)
                     /locus_tag="P5573_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
     gene            complement(426..896)
                     /locus_tag="P5573_01510"
     CDS             complement(426..896)
                     /locus_tag="P5573_01510"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRT26774.1"
                     /translation="MAHTLYLCPHCGAEIAVYRNPVPTVDIIIEIDDGIILILRKNPP
                     VGWAIPGGFVDYGESLEDAAVREAREETSLEITCLRQMHTYSNPDRDPRQHTISTVFI
                     AKGKGTPRAASDASDIGVFSRDDLPKPLMFDHRAILEDYFHLVEKGRAGLANSP"
     gene            1044..1454
                     /locus_tag="P5573_01515"
     CDS             1044..1454
                     /locus_tag="P5573_01515"
                     /inference="COORDINATES: protein motif:HMM:NF015567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRT26775.1"
                     /translation="MVGVLIITHGDLGRELINAAEMIKGPLEGVVFVSVNYSKGVEDL
                     KKEIKASIKKVDSGDGTLIFTDLFGGTPSNISLSFLKEGKVEVVTGVNLPMLLKLDMR
                     GEKKLSELASLIRDYGRKNIYLASEVLSKEVVSK"
     gene            1477..2010
                     /locus_tag="P5573_01520"
     CDS             1477..2010
                     /locus_tag="P5573_01520"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HRT26776.1"
                     /translation="MNVSLVRVDNRLIHGQILEAWVPYVQATCIVVADDDVANDFFRE
                     TVIRMAVPREIEVVITGVADFVKNHSYEDRSGEKTIILFSTLRAAVKAFNLGFHYDSL
                     NIGNVYEEEGAVQWARSIRLGERDISDLVTLNNAGVQIELRCVPQDKPVSFQESLNKA
                     KRKLPGLTAIKGRDVAP"
     gene            2000..2686
                     /locus_tag="P5573_01525"
     CDS             2000..2686
                     /locus_tag="P5573_01525"
                     /inference="COORDINATES: protein motif:HMM:NF015566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HRT26777.1"
                     /translation="MLLDVLTASILGGILCLDRVFVQAALSRPIVAGALVGLILGEPR
                     SGLLTGALVELFWVDQFQIGAYIPPNDSVAAIVITAGTVISGKALGGISKELVATAVL
                     LFIPLGILGRQMDIAIMRANDGLSRRALSDAVNGDLAGVEKKQLCGLAGYFLASVGFL
                     AVFTLVAEGTLLWVYPAIPEKVFPALKYVYSFLPLLGIAVALNTIKLKGMVPVFCAVF
                     LAVAVLIDLA"
     gene            2717..3217
                     /gene="rimI"
                     /locus_tag="P5573_01530"
     CDS             2717..3217
                     /gene="rimI"
                     /locus_tag="P5573_01530"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HRT26778.1"
                     /translation="MSTLEYVTLREMEEGDLDQVMAIETVSFRTPWTVNMFKSELRAR
                     ISRNLVVLAGACGRQEIAGYVSFWVFADESHLNSLAVRDDFRRRGVASKLLEAMVERS
                     KKEGALRGTLEVRESNQAAIALYERYGYVVAGRRRNYYDDTKEDALIMWCDFRHTMKR
                     TPSDDL"
     gene            3207..4025
                     /locus_tag="P5573_01535"
     CDS             3207..4025
                     /locus_tag="P5573_01535"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HRT26779.1"
                     /translation="MTCDASVKMPISGEVRKNQKVADDHYHLVLHAGKEYLSSSPGQF
                     LMIRLIGADVPLLGRPMSIYAVNEDGDGACVEVLYRVVGRGTRLMAQLKEGERVGVVG
                     PLGKGFTLYRDKKRVVLVAGGVGVAPLTFLAKAYSSLPKMRSVEVVSYIGARTGTCIV
                     GKGELGSIGRVSVSSDDGSCGHHGSVTDLFVRDLGAFEEDETVVYACGPSLMLKKMQE
                     ITSDRSFVVQVSLEERMACGIGACLGCVVRVKGGGYARVCTEGPVFDLKEVCFD"
     gene            4018..4953
                     /locus_tag="P5573_01540"
     CDS             4018..4953
                     /locus_tag="P5573_01540"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HRT26780.1"
                     /translation="MIDRADRPPRMEVTIGRGLKLKNPVMAASGTFGYGEEFARYVDL
                     NSLGAIVVKGLSLEPASGNPPPRIMETPCGMLNSIGLQNVGVRVFLAEKLPFLRNYDT
                     AVIANIFGETMDEYARLSEILSGAEGLKAVEVNVSCPNVEKGGIAFGSYPDRVAEVTR
                     AVKGSTDLPVIVKLSPNVTDIALIAEAAEGAGADAVSLINTLKGMSIDVERRVPHIAK
                     VVGGLSGPAIRPIALRMVWEVVNRVSIPVIGVGGIVDGSDALQFLIAGARAVQVGTAN
                     FMDPTATTKVLEGITDYMNRHYVRDINDLIGTLRV"
     gene            4983..5666
                     /locus_tag="P5573_01545"
     CDS             4983..5666
                     /locus_tag="P5573_01545"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRT26781.1"
                     /translation="MGKAEVIADGVYLVGGPGVSHSEDATAFVISFPEELVMIDSGAG
                     QSAGRLVKNIEDSGLDPGKISTIILTHCHIDHIGAAPYFRERFGSRLVVHELDAEAIE
                     DGDPVLTAANWYGTNFPPTPVDKKLKGEHEVLLFDKEELHCLHTPGHTPGSISVYLDR
                     GGKRILFGQDIHGPFLPSFKSNVILWRRSMEKLLALNADILCEGHYGIFSGRERAAAY
                     IRSQLRQVG"
     gene            6142..7467
                     /locus_tag="P5573_01550"
     CDS             6142..7467
                     /locus_tag="P5573_01550"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRT26782.1"
                     /translation="MTAFKDYLANKHTRPAPLKGLRVLEVCTLLFGPAGPAFLATMGA
                     EVIKVEMPPLGDVTRSLNPFGWFYKEQSPMFMHINPSKLYMGLDLHQEAGQEIFKELC
                     AKSDIVEFNLRPAVTKRWNIGYEEIRKINPGIIYIEKNGFGQWGQYAEEDRPSNDGAA
                     QALSGFAWISSFPKRPPLKQSIWVCDVYGALMGEVAVLAALHHRERTGKGQFIELSQT
                     ENIMRAMGWVWPYQQFTGKGAEPSGNRDQCICPADTFLCKDDQFAAIAAPASDEFKGL
                     CAAMGRKELAEDPRFKEHTERLKDENALAILKVIADWARTKTADEIEQLGIQHGFSAT
                     RLHSGKDMFEDPHRRQRGFVKMIDDPMYGYYPDHEFPVMMSKSPPRHRWTVRPVGFDN
                     DYIMTKILSRSKSQIEELYSIGVLGLWKDQQGRRPPPDWDGKSGAILRR"
     gene            7473..>8434
                     /locus_tag="P5573_01555"
     CDS             7473..>8434
                     /locus_tag="P5573_01555"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRT26783.1"
                     /translation="MAEYKRAKWADWADYIRSLDDPAKNFEKPEALDDIVVLDISSRS
                     MAGCYCSSLLSELGAETIRVEPPGGDLSRTYSPNGIMHQGTGLAYLQEGRNKYHITLN
                     LEKPEGRDILKTLVGNADVLIETYPTGTMDGWGIGYEELSKINPGLIFTSITAFGQYG
                     RESVRKQYDYDNVSQARSGIQYGTGEVLPEGKTPAEVPYAVPTKAGPWIAWATSGTFA
                     ALGILAALHYRNMTGEGQALDVATAEAYERFHDYALHWYADQGVLNERFGSLDTALWL
                     YGFYPTKDGGVFLGGLRLEMWKAFVDIIGKWDEWGAESWTTLAP"
BASE COUNT         1987 a         2017 c         2479 g         1951 t
ORIGIN      
        1 aatttgagat atcggccttg ctctactcac gggcggagag aaaagagtat atgaaacggt
       61 ttatctccga gctggaaagc aagaagaaac aaaggattca gtagatgtcg ttttcatagt
      121 ctgttgtgga aaaatcttta agctggtcac gccttttctt gtgctgtaat tttctcagtg
      181 cctttgattc aatctggcgt attctttctc gggtaacccc catcatttcg ccgacctctt
      241 cgagggtgaa aggcccgtaa ttacaggcca cccacgtgca gttgcagaaa gtttcgctct
      301 tcaaccacca ttcacatacg ttttcctggc agggttccgt tataaaggtg ttggtttttt
      361 tgcacttata gagatttgtc atggatgatc accttcctgc ggtaagttat tatatattat
      421 ggcctttacg gcgaatttgc aaggccggcc cttccttttt cgaccaggtg aaagtaatct
      481 tcgaggatag ctctgtggtc gaacattagc ggtttgggta aatcgtcccg tgaaaaaact
      541 ccgatgtctg atgcgtcgct cgcggcccgc ggtgtaccct tgcctttagc tatgaaaacg
      601 gtcgagattg tatgttgtcg agggtcgcgg tcggggttcg aataggtatg catttgccta
      661 aggcatgtta tctcgaggga agtttcctcc cgggcctctc tgacggctgc gtcttccaga
      721 gattcgccgt aatcgacaaa gccgcccgga atagcccaac cgacgggtgg gtttttccgc
      781 aggataagta tgatgccgtc atcgatttct atgatgatgt cgaccgtggg gactgggttg
      841 cggtaaacag cgatttctgc tccgcagtgc ggacacaggt aaagagtatg ggccatagaa
      901 gtgctccccc gaagaaagta aaataaaaac tattcatcca aagggttttt gtcaaggatt
      961 aagcctctgt catccgaact tcttgacaat cctatggcaa tcatttagct tacatccttc
     1021 agtttggcat ggggaggatg agcatggttg gtgttttgat aataacccac ggcgacctcg
     1081 gccgtgagtt gattaatgca gcagagatga taaaaggccc ccttgaaggt gtcgtgttcg
     1141 tctcagtcaa ctattcaaaa ggggtcgaag acttgaaaaa agagataaag gcatccataa
     1201 aaaaggtgga ttccggcgac ggtactctga tttttacgga cctcttcggc ggcactccgt
     1261 ccaatatatc cctgtctttc ttgaaagaag ggaaggtgga agtcgttacc ggtgtaaatc
     1321 tccccatgct gctgaagctc gacatgcggg gcgaaaagaa gctttcggaa ctcgcttcat
     1381 tgatcaggga ttacgggcgg aaaaatatct acctggcgag tgaagttctg agtaaagaag
     1441 tggtaagcaa gtagtcaact ccatgacggc aggcccatga atgtttccct ggtgcgagtc
     1501 gacaaccgcc tgatccacgg acagatcctt gaggcatggg ttccatatgt acaggcgaca
     1561 tgtattgtcg ttgctgacga cgacgtggcg aacgatttct tccgggaaac agtcatcagg
     1621 atggcggttc cccgggagat cgaagtcgtg atcaccggcg ttgcggattt tgttaagaat
     1681 cactcttacg aagatagatc gggggagaaa accatcatcc tcttcagtac tctccgggcg
     1741 gccgtaaaag ccttcaacct cggttttcat tatgacagcc tgaacatagg gaatgtctac
     1801 gaggaggagg gtgcggtcca atgggcaagg tccattcgtc ttggggaacg agacatcagc
     1861 gacttggtca cactgaacaa tgcgggtgta cagatagagc taaggtgtgt tccacaagat
     1921 aaacccgtca gttttcaaga atccctgaat aaggcaaagc gcaaattgcc cgggttgacg
     1981 gcgataaaag gaagagacgt tgctccttga cgtactgacg gcatccattc tcgggggaat
     2041 actctgcctg gacagggttt ttgtgcaggc cgcgttgtcc aggcccatcg ttgccggagc
     2101 ccttgtcggt ctgattcttg gcgaacctcg atccggttta ttgaccgggg cgctggtaga
     2161 acttttctgg gtcgaccagt ttcagatcgg cgcctatatc ccccccaatg attcggtcgc
     2221 ggcgatcgtc atcacggcgg gcacagtgat ctcgggaaag gccttggggg gtatatccaa
     2281 ggaactggtt gccacggcgg tgctgctctt cataccgctc ggcatcctcg gccggcagat
     2341 ggacattgca atcatgaggg cgaacgacgg tctttcgagg agggcactat ccgacgcggt
     2401 aaacggggat ctcgccggtg tcgaaaagaa acaactttgc ggcctggccg gatatttcct
     2461 ggcatcggtc ggttttctcg cggtctttac tctcgttgca gaaggcaccc ttctttgggt
     2521 ttatccggcg ataccggaaa aagttttccc ggcattaaaa tatgtatact ccttcttgcc
     2581 gctgcttgga atcgccgttg cattgaacac aatcaaacta aaagggatgg ttccggtatt
     2641 ctgtgctgta tttcttgcgg tcgccgtctt gattgatttg gcctgaaatt tttttatgac
     2701 gccttgtgaa atcggcatgt ctacccttga gtacgtgacc ttgcgggaga tggaggaagg
     2761 agaccttgat caagtcatgg ccatcgaaac ggtgtccttc cgtacgccat ggaccgtcaa
     2821 tatgtttaaa agtgagttgc gagcccggat atcacgaaat cttgtcgtcc tggccggagc
     2881 ctgcggaagg caggaaatag cgggatacgt gagcttttgg gtctttgccg acgagagtca
     2941 cttgaacagt ctagcggtcc gggacgattt tcgtaggagg ggggttgcgt cgaagttgct
     3001 tgaagccatg gtggaacgct cgaagaaaga gggcgccttg agagggactc tcgaggtaag
     3061 ggagagtaac caggcggcca tcgccctcta tgaaaggtac gggtacgtcg tcgcaggcag
     3121 gcggcgcaac tactacgatg acacgaagga agacgcgctg atcatgtggt gcgatttccg
     3181 tcataccatg aagaggacgc cctccgatga cctgtgatgc gagcgtgaag atgcccatca
     3241 gcggggaggt caggaagaac cagaaagttg cagacgacca ctatcacctc gtccttcatg
     3301 ccgggaaaga atacctctct tcatcgccgg gacaattctt gatgatacgc cttatcgggg
     3361 cggatgtgcc ccttcttggg aggccgatga gtatctatgc cgtgaacgaa gacggcgacg
     3421 gtgcctgcgt cgaggtactc taccgcgtgg tcggcagggg tacgaggcta atggcgcagt
     3481 tgaaagaggg tgagagggtg ggggttgtcg ggccgctcgg aaaaggcttt actctttacc
     3541 gtgacaaaaa acgagtcgtc ctcgtcgctg ggggagtggg cgtagcgccg ttgacgttcc
     3601 ttgccaaggc ttacagtagt ttgcctaaga tgaggtctgt cgaagtggtg agttacatag
     3661 gcgcccggac cggtacgtgc atcgtgggca aaggagagtt ggggagcatc gggcgagtca
     3721 gcgtcagcag tgacgacggc agttgcggcc accacggctc ggtaacggat ctttttgtga
     3781 gggacttggg tgcttttgaa gaagacgaga ccgttgtcta tgcctgcggt ccttctctca
     3841 tgctcaaaaa aatgcaagaa atcacaagtg atagaagctt cgtcgttcag gtgtcgctcg
     3901 aggaacgtat ggcctgcggg ataggtgctt gcctcggctg tgtcgtcaga gtgaaagggg
     3961 gaggatacgc gagggtatgc acggaaggac ccgtgtttga cctcaaggag gtctgctttg
     4021 attgaccgtg cagatagacc gccgagaatg gaggttacca tcggtcgcgg cctgaagctg
     4081 aaaaatcccg tcatggcggc atccgggacg ttcggttacg gcgaggagtt tgcccgttat
     4141 gtggacttga acagcttggg ggctattgtg gtgaagggcc tttcattgga gccggcaagc
     4201 gggaatccac cgccgagaat catggaaacg ccctgcggca tgctcaattc cataggttta
     4261 cagaatgtgg gcgtacgggt ttttctggcc gagaagctcc cctttttgag gaactatgat
     4321 acggcggtta tcgcgaacat attcggggag acgatggacg aatatgcgag gttgtctgag
     4381 attctaagcg gagctgaggg cttgaaggcg gtcgaagtca atgtatcctg tcccaacgtt
     4441 gaaaagggcg gtattgcctt cggctcctac ccagataggg tcgcagaggt gacgagggcg
     4501 gtaaagggtt cgacggacct gccggtcatc gtcaaattat cgccgaacgt gacggatata
     4561 gccctcatcg ccgaggctgc agaaggggcc ggagcggacg ccgtttccct gatcaacacg
     4621 ttgaagggta tgtcgatcga cgtggaaaga agggttcccc atatcgccaa ggtagtcgga
     4681 ggtctgtcag ggccggcaat cagacccatt gccttgcgga tggtatggga ggtggtcaat
     4741 cgggtatcga tccccgtgat aggtgtcgga gggatcgtcg acggaagcga cgcccttcaa
     4801 tttctcatag ccggagccag ggcggtgcag gtcggtacag ccaactttat ggaccctacg
     4861 gcaacgacta aggtcctgga aggaataacc gattacatga atcgccatta tgttcgtgat
     4921 attaatgact taatcggaac tctaagggta taagttttaa aaacagcaag tgtgaggttg
     4981 ctatgggtaa agcagaagtc atcgcggatg gcgtttatct tgtcggagga ccgggcgtat
     5041 cccattcgga ggatgcaacg gcttttgtca tctcatttcc ggaagaactc gtgatgatcg
     5101 acagcggcgc cgggcaaagt gcagggcgcc tcgtgaagaa tatagaggac tccggcctcg
     5161 acccgggcaa gatttcgacg ataattctga cccactgcca tatcgaccac ataggcgccg
     5221 ccccgtattt cagagaacgc ttcggcagtc ggttggtcgt gcatgagctc gatgccgagg
     5281 ccatcgagga tggtgacccg gtgttgacgg cggcaaattg gtacggaacg aacttcccgc
     5341 ctacgccggt ggacaaaaaa ctcaagggcg aacatgaggt gctgcttttc gataaagaag
     5401 agcttcactg tcttcatacg cccggccata cacccggatc tatctctgta tacctcgacc
     5461 gcggcgggaa acggatactc ttcgggcagg atattcacgg ccccttcctc ccaagtttca
     5521 agtcaaacgt catcctctgg cgtcggtcga tggaaaagct gctcgccctg aacgcagaca
     5581 tcctctgtga gggccattat ggaattttct ccggcaggga acgcgctgca gcctacatca
     5641 ggagtcagtt acgacaggtt ggttgacccc aagcagcatt aagcccttat gtttgctcac
     5701 tgtcggagcg ggtacctttc cctataccct gttctgctac gtcccttttt ttcttgcttt
     5761 taagccgtta tggtgttaac gaaatcatca gattttttac ccacatatcc tgtctaattg
     5821 taactatggt tcgattctta ttacattgac cttaaaattg tttctgtaat gtcggccttt
     5881 tcattagatt atcttaagag cttttcgaat aattaaccgc tgtcggctgt ctgatagacc
     5941 cttgatacgg tgatatccgc tggatagagg gaaaatagct gcagtaacgc ggagaatcgt
     6001 cgaggcatcg aggaaggtat aacgggaact attttcaagg gggaaatatg tccatgaagg
     6061 aggtgatgaa aaagcaactc agtttaatct cggacggttt ttttcgaaat ctttttaggg
     6121 gatttaagaa ggaggggaat aatgacggca ttcaaggatt atctggcaaa caaacataca
     6181 aggccggcgc cgctcaaagg gttgcgtgtc ctggaagtat gcaccctttt gttcgggccg
     6241 gcggggcctg ctttcctggc gacgatggga gctgaggtca tcaaggtcga aatgccgccg
     6301 ttgggagacg tgacgcgctc gcttaatccc ttcgggtggt tttacaaaga gcagagtccc
     6361 atgttcatgc acatcaatcc ctcgaaactc tatatgggcc tggaccttca ccaggaggcc
     6421 ggccaggaaa tctttaaaga actgtgcgcc aagtccgaca tcgtggagtt caacctccgg
     6481 ccggcagtca cgaagagatg gaacatcggc tacgaggaga tcaggaaaat caacccgggc
     6541 atcatctaca tagagaaaaa cgggttcggt cagtggggtc agtacgcaga ggaagacagg
     6601 ccgtcgaacg acggagcggc acaggccctt tccggtttcg cctggatctc gtcttttccc
     6661 aagcgaccgc ccctgaagca gtccatctgg gtctgtgacg tttacggagc cctgatggga
     6721 gaggtggccg ttcttgccgc cctccaccat agggagcgga cgggaaaggg acagtttata
     6781 gaattgtcgc agaccgagaa tatcatgcgc gccatgggct gggtgtggcc ctaccagcag
     6841 ttcacgggca agggagcgga gccctcggga aaccgcgacc agtgcatatg tccggcggac
     6901 acgttcctct gcaaagacga ccagtttgcg gcgatcgcgg cgccggcgtc cgacgaattt
     6961 aaagggctgt gtgcagccat gggccgcaag gaacttgctg aggacccaag attcaaggaa
     7021 cacacggagc gtctcaaaga cgagaacgct ttagcgatcc tgaaggtaat tgccgactgg
     7081 gcgcggacga agaccgccga cgagatcgaa cagctcggga tccagcacgg attctcggca
     7141 acccgcctac acagcggcaa ggatatgttc gaggatcccc acaggaggca gcggggtttc
     7201 gtcaagatga tagacgaccc gatgtacggg tattaccctg atcacgagtt cccggtgatg
     7261 atgtccaaga gtccaccaag acaccgctgg accgtcaggc ccgttggttt tgacaacgat
     7321 tacatcatga cgaagattct gagcaggtct aagtctcaga ttgaagagtt atattcgatc
     7381 ggagtcctcg gactatggaa ggaccagcag ggccgccgac cgcctcccga ttgggacggt
     7441 aaatccggtg caattttaag gaggtgatga caatggccga atacaaaaga gcaaaatggg
     7501 cggattgggc ggactacatc cggtcgcttg atgaccccgc gaagaatttc gagaagccgg
     7561 aggcgctgga tgatattgtc gttctcgata taagctccag aagcatggca ggttgctatt
     7621 gttcgtcact cctgtcggaa ctgggagcag aaacgatacg ggtcgagcct cccgggggcg
     7681 acctatcgag gacctattca cccaatggaa ttatgcacca gggtacgggc cttgcttacc
     7741 tccaggaagg aagaaacaag tatcacataa ctctcaacct ggaaaagccg gaaggtagag
     7801 acatcctgaa aactctcgtg ggtaatgcag acgtactgat cgagacctac cccaccggca
     7861 cgatggatgg ctggggcatc ggatacgagg aactcagcaa gatcaatccc gggctcattt
     7921 tcacgtcgat aacggccttc ggacagtacg gccgggaaag tgtgagaaag cagtacgatt
     7981 acgacaacgt gagtcaggct cggtcgggga tccagtacgg gacaggcgag gttttgccgg
     8041 aaggaaagac gccggcggaa gttccctatg ccgttccaac gaaggctggc ccctggatcg
     8101 catgggcgac ttctggaacc tttgccgcgc tcgggatact tgccgccctc cactaccgca
     8161 acatgaccgg agaagggcag gcccttgacg tcgcgacggc cgaggcttat gagagattcc
     8221 acgactacgc cctccactgg tatgcggacc aaggggtgct gaatgaacgc ttcggcagcc
     8281 tcgatacggc gctctggctt tacggcttct atccaacaaa ggatggagga gtgttcttgg
     8341 gcggattacg cctcgagatg tggaaggcct tcgtggatat catcggaaag tgggacgagt
     8401 ggggggctga gtcgtggaca acgctcgcgc cgtt
//
LOCUS       DAOTMX010000029         8324 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_7692_length_8324_cov_3.751602, whole genome shotgun sequence.
ACCESSION   DAOTMX010000029 DAOTMX010000000
VERSION     DAOTMX010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 8324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8324
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7692_length_8324_cov_3.751602"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            388..2088
                     /locus_tag="P5573_01560"
     CDS             388..2088
                     /locus_tag="P5573_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HRT26784.1"
                     /translation="MAQAPTAPPTDFISEIIEEDLKTNRYGGRVATRFPPEPNGYIHI
                     GHAKSVCLNFGLAARFGGTCNLRMDDTDPAGESEEYVNSIINDVRWLGFDWGDRLYFA
                     SDYFERLYDFAVRLIKMGKAYVCSLSPDEIREYRGSFNEPGKESPFRNRSVEENLDLF
                     TRMRAGEFEDGAHVLRAKIDMASPNIVMRDPIMYRIKREPHYRTGNEWVIYPMYDFAH
                     CLSDALENITHSICTLEFENNRPLYDWFLEQLIAGARPRQVEFARLNVSYTVLSKRRL
                     IELVEGRYVTGWDDPRMPTVAGLRRRGCTPEAIRNFCNAIGVAKNDNLVDIALLEHCV
                     REDLNEKAPRAMGVLRPLKVILDNYPEDKTEEFEVANHPQNPGMGTRKVPFSRVLYIE
                     RDDFDENPPKKYKRLSPGREVRLRNAYVIKYQNLVKDERTGEITELHCTFDPETRSGP
                     PPDGRKVEGVIHWVSAKHSVPAEVRLYDRLFRIPDPGSISDDYLDYLNPDSIKVLPNC
                     CVEASLRYAAPGNRYQFERIGYFCLDTKDSAPGKPVFNRIVSLRDTWSKIAGLKQGPQ
                     "
     gene            complement(2152..2304)
                     /locus_tag="P5573_01565"
                     /pseudo
     CDS             complement(2152..2304)
                     /locus_tag="P5573_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292462.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
     gene            complement(2428..2868)
                     /locus_tag="P5573_01570"
                     /pseudo
     CDS             complement(2428..2868)
                     /locus_tag="P5573_01570"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
     gene            complement(2933..4873)
                     /locus_tag="P5573_01575"
     CDS             complement(2933..4873)
                     /locus_tag="P5573_01575"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRT26785.1"
                     /translation="MFPKLFSEIKVGKVVLGNRIVMPAMGTNFAEDGMVSRRLTDYLA
                     RRAEGGAGLIITEAAMVVDYPGQKLSRYHLNISKDLFIPGLKDLADAVHRAGAKVALQ
                     LSHMGRQIHSSFIGTQPVAPSPIPCPVYKEIPRELTIDEIKSIIGDFVEGARRARDAG
                     FDMVELHACHGYLISEFFSLRSNRRTDVYGGNAENRARFCTEIIEGIKARLGKDFPVI
                     VRMNGHDYIDGGATLEGMKEIAPFLVYAGADALSISAGVYGSYVATVAPMYEKQGCFA
                     HLAAGIKEVVNVPVISAGRITDPAMAERILETGQADLVAMGRALLADPDLPHKARKGL
                     VEDIAYCTGCNQGCIDRINMSMIFGETRGITCLVNPCVGRERECELRKTDRPKKILVV
                     GAGPGGLETAMIAARRGHRVEVWEKDSRPGGQLSLAGRVPGQEAFADFVAYLERQAGK
                     AGAAISYNRAATADLVSGFSPEAVVVATGAAPIIPSFIKSGNTRLFTAWEILRGKDVK
                     GEQFIVIGGGGVGLETAHYLAVRGKKVKVLEAMSRVGRDLGPIVSSYLRRILLQEGVE
                     VICDVEVRSVENDVIRMTKEGREIVYDQVDGIIVAVGTRPDNSLVESLFGYPNVHVIG
                     DARRPAKALDAINEAFDLGLRL"
     gene            complement(4892..5674)
                     /locus_tag="P5573_01580"
     CDS             complement(4892..5674)
                     /locus_tag="P5573_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HRT26786.1"
                     /translation="MIGAIAGDIIGSVYEWNRIKTKTFPLFSPRCTFTDDSVLTIALA
                     DAILNGADYGEKMKEYYRRYPDAGYGGLFHSWARSRESRPYNSWGNGAAMRVSPVGFA
                     FDSLEDVLEKAKLYTEITHNHPEGVKGAQATAAAIFLARTGHSKREIREYVEAAFGYD
                     LSRALDEIRPGYRFDESCQGTVPEALTAFLESTDFEDAVRNAVSLGGDSDTLACITGG
                     IAQAFYGGVPDFISRKVYSVLDDELGRVTRQFMDRYVQILIS"
     gene            complement(5714..6499)
                     /locus_tag="P5573_01585"
     CDS             complement(5714..6499)
                     /locus_tag="P5573_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HRT26787.1"
                     /translation="MKAAFVQFNPLFGDVEGNTAKAVAMVEKTEADVIVLPELFNTGY
                     LIVSQEEAFDLSEPVPGGRTTEALCNAARKKGSHIVAGLIERQGDDLFNSAVIIGPTG
                     YLGTYRKIHLFNEEKLWFKPGDLGFSVYDIGSCKIGVMVCFDWFFPESMRTITLCGAD
                     LICHPANLVLPYCQDAMTTRCLENRVFAVTANRTGEERRDGKTFRYTGASQITSPDAK
                     VLYRACADTEEVGVAEIDLALARNKNLNPYNHILRDRREEFYK"
     gene            complement(6558..6944)
                     /locus_tag="P5573_01590"
     CDS             complement(6558..6944)
                     /locus_tag="P5573_01590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRT26788.1"
                     /translation="MTGERKVISTPDAPKAIGPYAQAVRYGDLLFVSGMIAIDPKTGV
                     LLTGDFEAEVIRVLENIKAVVAAGGMSLENVLKVTVFLKDLGNFAKFNEIYGRYFGGI
                     LPARETVQAAQLPGNASVEISVICGK"
     gene            complement(7039..7770)
                     /locus_tag="P5573_01595"
     CDS             complement(7039..7770)
                     /locus_tag="P5573_01595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRT26789.1"
                     /translation="MGNQSDANEQLVRNLQNRVEELKRLVTNLTEAAMLSRDRELSWI
                     YALEGSRDGVWDWNAVTNEVFFSKRWKEMLGFAEEEISNHLSEWEKRVHPDDRDSVYA
                     DLNAHLNGQTPYYENEHRLLCRDGYYKWILDRGKVMSWTEDGKPLRVVGTHSDITLRK
                     EVELENQRLLKELREALANLKVVRGLLPICASCKKIRDDKGYWNELESYIGAHSEADF
                     SHSICPDCARKLYPAAVTHGKKNVW"
     gene            complement(7920..8264)
                     /locus_tag="P5573_01600"
     CDS             complement(7920..8264)
                     /locus_tag="P5573_01600"
                     /inference="COORDINATES: protein motif:HMM:NF014672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HRT26790.1"
                     /translation="MSEKKHLYVLWTNDNLITAEKMVFMYTVNSLVRSWWEEVTLIIW
                     GATAKLVGENADIQKKVKEAMDAGVHVTACKACSDQLGVTQTLEDLKIEVKYWGAPLT
                     EIIAKGEKLLTI"
BASE COUNT         1685 a         2497 c         2291 g         1851 t
ORIGIN      
        1 tgccgccgag tccttgaagc ggaagtaatc gcaaaaattg cccttttctt tttcgagcac
       61 ccgctcggac tggggttccc tgcactgatt gtaggcgccg gtgtcgtaga acgcgcagtt
      121 catgcagcat cgcaggtctg cgcggcagaa gggacaagtg tcgttacgac ccggcggact
      181 gcccagtgtc aattcgttgc ggcagttaaa gcatgttttc atgttgaccc ttaggggctg
      241 ccccttttaa cggccgagtc tttggtcgaa agccattttc gattaaacag gccgggaagt
      301 caaggaaagt tgcggtttac tgctggcgcc gcaaagcgag atagggtata agagggccaa
      361 ttctcggcag caaggagatt gagagcgatg gcgcaagcac cgacagcacc tccgaccgat
      421 ttcatcagcg agatcatcga ggaggacttg aagacgaaca gatacggggg gcgcgtcgcc
      481 acgcgtttcc cacccgaacc gaacggctac atccatatcg gccacgccaa atcggtctgc
      541 ctcaatttcg ggctggccgc ccgcttcgga gggacctgca acctgcggat ggacgacacc
      601 gacccggcgg gggaatcaga ggagtacgtc aattccatca tcaacgacgt ccgctggctg
      661 ggcttcgact ggggcgaccg cctgtacttc gcctccgatt atttcgaacg cctgtacgac
      721 tttgccgttc gcttgatcaa gatgggaaag gcctacgtct gcagcctgag ccccgatgaa
      781 atccgggagt accgggggag tttcaacgag ccgggcaagg aaagcccctt cagaaaccgc
      841 tccgtagagg agaacctgga tctcttcaca cggatgcggg cgggtgagtt tgaggacggc
      901 gcccacgtcc ttcgcgcaaa gatcgacatg gcgtcgccaa acatcgtgat gcgggacccc
      961 atcatgtacc ggataaagcg tgagccccat taccggacgg ggaacgaatg ggtcatctac
     1021 cccatgtacg acttcgccca ctgcctatcc gacgccctcg agaatatcac ccattcgatc
     1081 tgcacgctgg agttcgagaa taaccgcccc ctgtacgact ggttcttgga gcagctgatc
     1141 gcgggagcac gcccgaggca ggtcgaattc gcccgtctga acgtcagtta taccgtcctc
     1201 agcaaacgga ggctgatcga actcgtggaa ggcaggtacg tgaccggttg ggacgaccct
     1261 cggatgccca cggttgcagg tttgcggcgc cggggctgta cgccggaagc catccgtaat
     1321 ttctgcaacg caatcggggt ggcgaagaac gataaccttg tcgatatagc gctgctcgaa
     1381 cactgtgtcc gggaggacct gaacgaaaag gcgccgcggg cgatgggcgt gctccgaccg
     1441 cttaaggtca tcctcgacaa ttaccccgaa gacaagacgg aagaattcga agtcgccaac
     1501 catccccaga acccggggat gggaacgaga aaggttcctt tctcgcgggt gctctacata
     1561 gagcgcgacg atttcgacga gaatccgccg aagaaataca agcgcctcag ccccgggcgg
     1621 gaagtccgtc tccggaatgc ttacgtcatc aagtatcaga atctggtgaa agacgagcgc
     1681 accggagaga tcaccgagct tcactgcact ttcgacccgg agacgcgaag cggaccgcct
     1741 cccgacggac gcaaggtaga gggtgtcatc cactgggtat cggcgaaaca ctcggtgccc
     1801 gccgaggtaa gactctatga ccgcctcttc cgcatccccg accccgggag catttcagac
     1861 gactaccttg actacctgaa cccggactcc atcaaggtac tgccgaactg ctgcgtcgag
     1921 gcaagcctga gatatgcggc gccgggtaac cgttaccagt tcgagaggat cggttacttc
     1981 tgcctggata cgaaggattc ggcaccgggg aagcctgttt ttaaccgcat cgtctccctg
     2041 cgtgacacct ggagcaagat cgccggctta aaacagggtc cgcagtgatc gcttgaaggc
     2101 gatgagattc gcagggggct cgcggatggg acgcaggata cgtgcgcttc cttactccgg
     2161 tgtaaagacg gatttgtcgg acccgcagac agggcacacc catccttccg gcaggtcctc
     2221 gaagggaaca tcggcagcga tcccgccttc ggcatcgccc ttttccgagt cgtacacgta
     2281 gccgcagacc gagcagacat agcgcttctt cgccgccgct ggcgccttcg gctggtcctg
     2341 ttgataagtg ggggcggcct tgggcgactt gccccctctc acgttgtggt agtagtcgta
     2401 ggtcatacac ggcccgtcgg cgactatgtc gcagtccagc acctctccga cgaagatagt
     2461 gtgcgttcca cagtcgagct gctgtttcac cgagacctcg atcacggcaa tcgtgtggtc
     2521 gagcaagatg ggtgcgcccg actctcccct gcggtaagaa acgccctcga atttcttcgt
     2581 ctcgcggccg ctcttgaaac cgaagcgacc gatcagctcc atgggggccg actgagcgag
     2641 gatcgagaca gagaaattgc cgctatcgct tatgaacgag tgtgtcaggt tctccttgtt
     2701 aatgctgacg gcgatcgtcg ccggcgtgga ggtcacctga atgaccgtgt tggcgatctg
     2761 tccgttgagc tcaccatcac gggtcgcgcc gatcacgtac agaccgtagc taatcttgtg
     2821 taaggcgaat ctgttcgttg ttcgtctcct attgaggttc tccgccactt gcggaattcg
     2881 ttctgggacg gcgcatgccc ggcatggttg tcggcgcgct ccatccgtgt ttttataatc
     2941 tcaggccgag gtcaaacgct tcattgatcg cgtcgagggc cttggccggt ctacgggcgt
     3001 caccgattac gtgaacgttg ggatagccga ataatgactc aacgagtgaa ttatccgggc
     3061 gggtacccac cgccactatt atcccgtcca cctggtcgta cacgatttct cggccctctt
     3121 ttgtcatgcg tataacgtca ttctcgacgc tcctgacctc tacgtcgcaa atgacctcga
     3181 cgccttcctg taagagtatg cgcctcaggt aggacgacac tatcggcccc aggtcgcggc
     3241 cgacccggct catcgcctca aggaccttca ccttctttcc ccttacggcc aggtagtggg
     3301 ccgtctcgag cccaacgccc ccgccgccga tgacgataaa ctgttcaccc ttgacgtcct
     3361 tccccctcaa tatctcccat gccgtaaaaa gcctggtgtt gccgctcttg atgaacgatg
     3421 ggatgatggg tgccgcaccc gtcgcgacta caaccgcttc gggtgaaaaa ccggatacaa
     3481 gatcagccgt cgccgcccga ttgtacgaaa tagcagcccc ggccttcccg gcctgtcttt
     3541 ccaggtaggc aacgaagtcg gcaaacgcct cttggcccgg gacgcgcccg gcaagggaca
     3601 attgcccgcc ggggcgggaa tctttctccc acacttccac ccgatggccg cgcctcgccg
     3661 cgatcatcgc cgtctccaga ccgcccgggc cggcacccac gaccagaatc ttcttcgggc
     3721 ggtcagtctt ccggagttcg cattcccttt ccctgccgac gcaggggttg acgagacagg
     3781 tgatgcccct cgtttcgccg aatatcatgc tcatgttgat cctgtcgatg cagccctggt
     3841 tgcaaccagt gcaatatgcg atgtcctcga caagaccttt ccgggccttg tgcggcaggt
     3901 ccgggtcggc aaggagtgca cgccccatgg ccactaagtc ggcctggccc gtctccagga
     3961 tgcgctcggc catagccggg tcggtgatcc ggccggcaga gatgactggg acattcacga
     4021 cctccttgat gccggcggca agatgggcga agcaaccctg cttttcatac atcggcgcca
     4081 ccgttgcgac gtaagagcca tagacgccgg cggagatgct cagcgcatcg gcccccgcat
     4141 agacaagaaa aggtgcgatc tctttcatgc cttcgagtgt cgcccctccg tcgatgtagt
     4201 cgtggccgtt catgcggacg atgaccggga agtccttccc cagtcgcgcc ttgatcccct
     4261 cgatgatctc cgtgcagaag cgggctcggt tttcggcgtt tccgccgtaa acatcggtcc
     4321 gccggtttga gcggagagaa aaaaattcgc ttatgagata gccgtgacag gcgtgaagtt
     4381 ccaccatatc gaagccggca tcgcgggcgc gcctggcccc ttcaacgaaa tcgccgatga
     4441 tcgatttaat ctcatcgata gtgagttcac gaggaatttc cttatacacg gggcagggta
     4501 tcggagaggg cgccaccggc tgagtgccga tgaagctcga gtgtatctgc ctgcccatat
     4561 ggctcagttg aagggcaacc tttgctccgg cgcggtggac ggcgtccgcc agatccttca
     4621 gtcccggaat aaagagatcc ttcgagatat tcagatgata gcggctcaac ttctggccgg
     4681 gatagtccac gaccatggcc gcctccgtga tgatcaggcc cgctccgccc tctgccctcc
     4741 gggcgagata atcggtcagg cgccgggaga ccatgccgtc ttccgcgaaa ttcgtcccca
     4801 tggccggcat cacgatgcgg tttccgagta cgaccttccc gaccttgatt tctgaaaaaa
     4861 gctttggaaa catgttcact tacgaaacct ctcaactaat caatatctgt acgtatctat
     4921 ccatgaattg ccgggtgacc cgtcccagtt catcgtcgag gacactatag accttccggc
     4981 tgatgaaatc cgggacgccg ccgtagaagg cctgggcgat gccgccggtt atgcacgcca
     5041 gggtatcgct gtccccgccc agggagacgg cgttccggac ggcgtcttca aaatcagtcg
     5101 attcaaggaa ggccgtcaat gcctccggca ccgtcccctg acaagattcg tcgaatcggt
     5161 aaccgggcct gatctcgtcg agggctcttg aaaggtcgta cccaaaagcg gcctcgacgt
     5221 attcccttat ttccctttta gaatgccctg ttcgcgcgag gaatatcgct gcggccgtgg
     5281 cctgtgcccc tttgacgccc tcggggtggt tgtgcgttat ttcggtgtac agttttgcct
     5341 tctccaggac gtcttccagg ctgtcgaagg cgaagccgac gggactcacc ctcatggcgg
     5401 caccgtttcc ccagctgttg taaggacggc tctcccggct cctcgcccag ctatggaaga
     5461 ggccgccgta acctgcgtcg ggataccgcc ggtagtattc tttcatcttc tccccgtagt
     5521 ccgcgccgtt aaggatggcg tccgccaggg cgatcgtcag gacgctgtcg tccgtgaacg
     5581 tgcaacgggg actgaaaagc gggaaggtct tggttttgat tcggttccat tcataaacgg
     5641 acccgatgat gtctcccgcg atggctccga tcatatcaag tacctgccgc tttctgtggt
     5701 tgtaaaatca ctctcactta tagaactctt cccgtctgtc gcgcagtata tggttgtagg
     5761 ggttcaggtt tttgttccgg gcgagggcga ggtcaatctc ggcgacgccg acctcctccg
     5821 tatccgcaca cgcccggtaa aggacttttg catccgggga agtgatctgg cttgctcccg
     5881 tgtaccgaaa cgtcttaccg tctctccgtt cttcaccggt tcggttggcc gtcacggcga
     5941 agaccctgtt ttcgaggcac cgggtggtca tcgcgtcctg acagtaggga agcacgaggt
     6001 tcgcggggtg gcagatcaag tcagccccgc agagggtaat ggtccgcatg gattcgggga
     6061 agaaccagtc gaagcagacc atgaccccga ttttgcaact gccgatgtca tagacggaaa
     6121 acccgaggtc ccccggtttg aaccagagct tctcttcgtt gaagagatgg atctttctgt
     6181 acgtaccgag gtaccccgtc gggccgatga tgacggccga gttgaagagg tcgtccccct
     6241 gcctttcgat caaccccgcg acgatatgtg agcccttctt gcgcgcggcg ttgcacaggg
     6301 cctcggtggt cctgccgccc ggcacgggct cggaaaggtc gaaggcctct tcctgagaaa
     6361 cgatcaaata gcccgtgttg aagagttccg gcagaacgat aacgtccgcc tcggtcttct
     6421 cgaccatcgc gaccgcctta gcggtgttgc cttccacatc gccgaagagg ggattgaact
     6481 ggacaaacgc cgctttcatg cctgatccgc ctccttaaaa agattccttt tatcctgacc
     6541 ctgattccgc ctacccgtca tttcccacag atgacggaaa tttcgaccga tgcgttgccg
     6601 ggaagttgcg cggcctggac ggtctcacgg gcggggagga taccgccgaa gtacctcccg
     6661 tagatttcgt tgaattttgc gaaattgccc aggtccttga gaaagaccgt tactttcagg
     6721 acgttttcga ggctcatccc gccggccgcg accacggcct tgatgttttc caggacacgg
     6781 atgacctcgg cctcgaagtc tcccgtcaag aggacccccg tcttcgggtc gatagcgatc
     6841 atacccgaga cgaaaaggag gtcgccgtac cgaacggcct gtgcataagg cccgatggcc
     6901 ttcggggcat cgggtgtgct gataactttc ctttccccgg tcataaggat acttctttcc
     6961 tgatcctgtt atttttgtaa cgccgttcgt tcgagcaccc atacggtaaa gggcatctct
     7021 accgctcggc tgactcgcct accatacatt ctttttgccg tgagttacgg cagctgggta
     7081 tagttttctt gcgcagtccg ggcatatgct gtggctgaag tccgcctctg aatgggcccc
     7141 gatgtaactt tcgagctcat tccagtagcc cttgtcgtcc cgtatctttt tgcaggatgc
     7201 acagatgggc agaagccctc tcacgacttt caagttggca agcgcctcgc gcaattcttt
     7261 caagagacgc tggttctcca gctcaacttc cttcctcagg gtgatgtcgg aatgtgttcc
     7321 cacaacgcga aggggcttac cgtcttccgt ccagctcatg accttccccc ggtcgagaat
     7381 ccatttgtag taaccgtctc tgcacagaag cctatgttcg ttctcgtagt aaggggtctg
     7441 cccattcaga tgggcgttga ggtctgcgta taccgaatcc ctgtcgtcag ggtgaacccg
     7501 tttctcccat tccgacaagt ggttcgatat ttcttcctcg gcgaaaccaa gcatctcttt
     7561 ccacctcttc gaaaagaaga cctcattggt cacggcattc cagtcccata cgccgtcacg
     7621 actcccctcc aaggcataaa tccagcttaa ttccctatcc cgggaaagca tggccgcctc
     7681 ggtaagattg gtcaccaaac gcttcaattc ctcaacccgg ttttggaggt tcctgactaa
     7741 ttgctcattg gcgtcacttt ggttgcccat actcatctcc agtaaactcg cagagcacaa
     7801 tgcatgatcc tcgactccgt gggcggggga taattccgcc gtcaaacatc acgaccggta
     7861 taaccgaccg gcaaaggaac gcggcggaag aaaacccccg gctaccccgt gaatcccttt
     7921 catattgtaa gcagtttttc tccctttgct atgatctccg tgaggggggc cccccaatac
     7981 ttgacttcga tcttgagatc ttccagggtc tgcgtcacgc ctaactggtc tgaacaggcc
     8041 ttgcaggcag ttacgtggac accggcatcc attgcctcct ttaccttttt ttggatgtct
     8101 gcgttctcac ccaccaattt tgcagtcgct ccccagatga taagagtcac ctcttcccac
     8161 caactacgga caagagaatt tacagtgtac atgaacacca ttttttcggc tgtaatcagg
     8221 ttgtcattgg tccatagcac gtaaagatgt tttttctcgc tcatcgacgg cctcctttca
     8281 ttctattcgg ggtttaacgc tctgtataac cggctaaccg acaa
//
LOCUS       DAOTMX010000030         8284 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_7734_length_8284_cov_3.551343, whole genome shotgun sequence.
ACCESSION   DAOTMX010000030 DAOTMX010000000
VERSION     DAOTMX010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 8284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8284
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7734_length_8284_cov_3.551343"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1087
                     /locus_tag="P5573_01605"
     CDS             <1..1087
                     /locus_tag="P5573_01605"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF036799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HRT26791.1"
                     /translation="DFLVTPPTFRVDVSREIDLIEEVARIRGYDGIPESVPPAGPRKD
                     LKDRKKVLGEKVIAFMNGYGYSEVINYSFTTPTSADILGLRGDDGGWGFVRIKNPLTE
                     DQSVMRVTLVYGLLETMKKNINNGSFDLKLFEKGKVFIPKAGDVELPRESERLGALLT
                     GSRYDQLWHSKGVQVDFYDVKGCLENLLAMLRIENVRFAPSADVEFLHPGRACRVLID
                     GKEAGIVGEVHPDVLERMDMKRRAMVFEVDLDLLADRFTEGVVFKEIKKFPAVYRDVA
                     FLVGADMEAEKLMDLAKAGEEELLEKIEVFDVYRGEKVPKDMKSIALRFTYRSDERTL
                     TDEEVARVHGGIVQRILSATGARVRGE"
     gene            1113..1391
                     /locus_tag="P5573_01610"
     CDS             1113..1391
                     /locus_tag="P5573_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HRT26792.1"
                     /translation="MTKVDIIQNVYDRLGFSKKDAARIVESIFDIMKERLEKGEKIKI
                     SGFGNFVVKEKKARRGRNPQTGTEIEISPRKVLTFKSSQVLRKALNHK"
     gene            1524..1862
                     /locus_tag="P5573_01615"
     CDS             1524..1862
                     /locus_tag="P5573_01615"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRT26793.1"
                     /translation="MSEPLPDKNFFRIGEVSKILQVKPYVVRYWESEFKTVKPIRTSA
                     DQRLYRRQDVEELLTIKNLLYHEGFTIAGARNKLRELKRGRTEGREEDDRSRLMTIKQ
                     GLKELRKLLD"
     gene            complement(1965..2204)
                     /locus_tag="P5573_01620"
     CDS             complement(1965..2204)
                     /locus_tag="P5573_01620"
                     /inference="COORDINATES: protein motif:HMM:NF038144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PxxKW family cysteine-rich protein"
                     /protein_id="HRT26794.1"
                     /translation="MTKKGCGFNGGTCNAIIDKCEGCGKIIESSFGRFCTVYPDPAAK
                     WSHGSCPTATHVKKVKEEASQKINPLKASKRAQKH"
     gene            2487..3239
                     /locus_tag="P5573_01625"
     CDS             2487..3239
                     /locus_tag="P5573_01625"
                     /inference="COORDINATES: protein motif:HMM:NF015570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HRT26795.1"
                     /translation="MNKETGRRMMNVFFRSFCIQSTWNFWRMQNLGFAFAMIPSIRTL
                     STDRDIRSRMLVRHLQMFNTNPYMSGPIIGSAMRLEEALCSDGNNPEVDQLKAALMAP
                     YAAIGDSFFWGALRPLAAAVGVLLALKGFFIAFLAPILIFNPFNLWVRLKGLVEGYRR
                     GKEGIEFIRSMNLLGMARQLRWVSVVFLAALALVVSSQTEWPFVQGLDILKRVFLLAV
                     ILACLWALKKGVSAVTILYSVSFLLFLVIGLR"
     gene            3236..3508
                     /locus_tag="P5573_01630"
     CDS             3236..3508
                     /locus_tag="P5573_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HRT26796.1"
                     /translation="MTTSLRIFTITNELGIHARSAALIVDVASRFRSEILIEKDGREV
                     NAKSILGILTLGAPKGSKITVRAEGPDAQEAVDSLGHLIENKFGES"
     gene            3508..5289
                     /gene="ptsP"
                     /locus_tag="P5573_01635"
     CDS             3508..5289
                     /gene="ptsP"
                     /locus_tag="P5573_01635"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HRT26797.1"
                     /translation="MGERDSQRKTHVLKGIGVSPGVATGKALILDYSIANVPCYQLGS
                     EAETLVEISRFEKALKESRRQLAVLRKKLSDREGPKPLFIMDVHMMMLKDKTLINNTV
                     NVIKERMINAEWALMLTIDKYREIFEKIDDEYLRERFSDVEYVGQRILRNLVGREHIR
                     LADIEEASIIISKDLSPADTVQMKVENILGFATDMGGKTSHTAIVARSIGIPAVVGLE
                     RVTREVRPGDIVVVDGLDGFVIVNPDPELIRRYEEKKRHFEILEEGLLREARLPAVTR
                     DNRKVDISANIEFVEEIPSAVAHGAEGVGLYRTEFIYINREQLPTEDEHFVNYRSVIE
                     GQGIKWSTIRTFDLGGDKFISDPKLAKEMNPVMGLRAIRFCLQEIELFKVQLRAILRA
                     SAYGKTRVLFPMISSLEEIIETKKIFGEVKEDLIRRGVPVGEDIELGIMIEVPSAVII
                     ADSLAREVDFFSIGTNDLTQYTLAIDRVNERVTYLYEPLHPAVLKLIKMIVDAGHRAG
                     IRVSMCGEMAGEPTYTLVLLGFELDELSMNPLAIPRVKKIIRNATFEESQALLNKVMT
                     FSTAREIEAYVRDYMTGRFPEDFRQED"
     gene            5305..7944
                     /locus_tag="P5573_01640"
     CDS             5305..7944
                     /locus_tag="P5573_01640"
                     /inference="COORDINATES: protein motif:HMM:NF039580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HRT26798.1"
                     /translation="MTTNVASEKKAGKKEYGCYGGSLYDSSGFSLSRFLERILSRVGF
                     SDEAAEELRSLHQRGVVVYAQKNASQLDALILREVLKKKGIPPPIYCHAVNMVAWQPL
                     FTALKCAFTRIFNLVFKKDSLNPYKTEYLKEITKKGASSLIYLRDSDFLGSPYPKDPL
                     IQLIHAQKEMETPVFIVPVGFFYGKRREKKNKTIMEILLGHVENPDPLRRVITFLRYS
                     KKATVVPAMPVNLTEYLESANPPRSYESISYNLRRELIDRINEERRAVFGPVLKSREE
                     IIVTVLRDAYLVKAMEELATATKKDFKAVFKEAKKYLKEIAADYQDIYIDIWYKILTW
                     LWNNIYDGVVVDREGLAMIRDISKRMPFVIVPCHRSHIDYLLLSYVFEVNNIPLPFVA
                     AGTNLMFWPLGPIFRKSGAFFVRRGFKGNVLYRQVFAKYIKVLLKEGLPIEFFIEGGR
                     SRTGKMVMPRYGMLSMVIQAYKEGISSDLAVIPVFIGYDRVMEEKAYLEELGGAQKTR
                     EKATDVIKSGGLLRRRYGRVYVNIGKPIFLKSYMASLEKTYEDMTVEERQSFYRKIGY
                     EVVGEINKVSVVTPSALVAAAMLCHYRRGISHDELVGIINELYEYLAYRNVKFASTFA
                     NRERALADALSLFETAGLISKMGIEEEDRNEEVEEIVYSLDEEKRLNLEYYKNNILHF
                     FVPLSFVATSILSSSEDAILFYRILEDYKFFRRLFKNEFIFDDKVDDVEEVNQVLNYS
                     HDRGMIEGKERDNEAWIEVKGKGRVGLRPYAGLIHNYIESYWVVIRGCSYLKKGARTE
                     REFTKKIQQLGTRMYKKGEIMRAEALSSSNYKNAVKFLCDMEIIFLRREKDKKDRKET
                     VVYSLTDDRSEVESLRQRLFKFL"
     gene            complement(8011..>8284)
                     /locus_tag="P5573_01645"
     CDS             complement(8011..>8284)
                     /locus_tag="P5573_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT26799.1"
                     /translation="LPLQGKAGPDGLPLGKKGEEIPLFGRIVAIADVFDALTARRSYK
                     HAWGESEALEAIEKASGRQFDPELVGILFSCLDSIRQARQRYPDEV"
BASE COUNT         2064 a         1988 c         2386 g         1846 t
ORIGIN      
        1 agactttctc gtgacaccgc cgactttcag ggtcgatgtc tcccgtgaga tagacctgat
       61 agaggaggtg gcaaggattc gaggttacga cggcatcccg gaatcggttc ctcccgccgg
      121 accacgcaag gacctgaaag acaggaagaa ggtcctcggt gagaaggtga tcgcttttat
      181 gaacggttat gggtattcgg aggtgatcaa ttacagtttt accaccccga cttccgcgga
      241 catccttggg ttgagaggtg acgacggcgg gtggggtttt gtgaggatca agaaccccct
      301 gacggaagac caatcggtga tgagggtcac tctggtttac ggcctcctcg aaacgatgaa
      361 aaagaacata aacaacggtt ctttcgacct caaattattt gagaaaggaa aggtctttat
      421 cccgaaggcg ggagacgtag aacttccccg ggaaagcgaa aggctcggtg cccttctcac
      481 tggttcgagg tacgaccagt tatggcattc taaaggcgtg caagttgatt tctatgacgt
      541 gaaagggtgc ctggaaaatc tgctggcgat gctcaggatt gaaaacgttc gttttgcgcc
      601 gtcggccgac gtggaattcc ttcatcctgg gcgtgcctgt cgggtgctca tagacgggaa
      661 agaggcgggt atagtcggag aagttcaccc cgacgtgctg gaacggatgg atatgaagcg
      721 tcgggcgatg gtttttgaag ttgacctcga tctcctggct gatcgtttca ccgaaggagt
      781 cgtcttcaag gagatcaaga aatttcccgc ggtgtatcga gatgtggcct tcctcgtcgg
      841 ggcggacatg gaggcagaga aacttatgga tctggccaag gccggagaag aagaattgct
      901 tgaaaaaata gaagtttttg atgtatacag gggcgaaaag gttcccaagg acatgaaaag
      961 catagccttg agattcacct atcgctcgga tgaaaggact ttgacggacg aagaagtggc
     1021 ccgtgttcac ggcgggatcg tacagaggat cctcagtgct acgggggcaa gggtgagggg
     1081 agagtagatt tgtcagaaaa gaggagggaa agatgacaaa ggtcgatatc attcagaatg
     1141 tttatgaccg gttaggtttt tcaaagaagg atgcggcccg gatcgtggaa tcgatttttg
     1201 acatcatgaa agaacgcctc gaaaaggggg aaaagatcaa gatttccggg ttcggcaatt
     1261 ttgtcgtaaa ggagaagaag gccagaagag ggagaaaccc ccaaaccgga acagaaatcg
     1321 agatatcgcc gcggaaggta ttgaccttca agtcaagtca ggtgttgaga aaggccttga
     1381 accacaagta gttccgtcta tttcccagta tttatcatag gttagaaaat cccttcattg
     1441 acgaaggctg atagtgacca gggttgcgga agaagatggt cggtcaccta aaggacgcgt
     1501 caattctccg acgtgaggtc ttcatgagtg aaccgcttcc cgacaagaat tttttccgga
     1561 tcggtgaagt aagcaagata ctccaggtga agccctatgt cgtccgttat tgggagtctg
     1621 agttcaagac agtgaagcct attcgtacga gcgccgatca gcgtctgtac aggcggcagg
     1681 atgtcgagga actgctcacc atcaagaacc ttctttacca tgagggtttc accatcgccg
     1741 gggcacggaa taagctgaga gagttgaaac gcggccggac cgaaggtcga gaagaggatg
     1801 acaggtctcg gctcatgacg atcaagcagg gccttaagga attgaggaag ttgctcgatt
     1861 gatgagtgac ggcaggagga aacacaagtt aggccggctg gagagggacc cctacagccg
     1921 gcttttttaa cagatgaaga aaggccgaga ctcgaggctg taccttaatg tttctgggct
     1981 cgcttcgatg ccttgagtgg attaattttt tgcgatgcct cttctttcac cttcttgacg
     2041 tgagtggcgg tcgggcagct gccgtgtgac catttggccg ccgggtccgg gtagactgta
     2101 caaaacctgc caaaagagct ttcgattatc ttgccgcagc cctcacactt atcgattatt
     2161 gcgttacagg tcccaccgtt gaacccacaa cccttcttag tcatgaatgc acactcaatg
     2221 ccggccttta ccgtttgaca aaccatcgga atcaccccct tcttattatt tgtttgcttt
     2281 tcttggttca aaaaaaggcg tcaatcccaa gaccggactg gacgccgtcg gatatccctc
     2341 aatcagggcg ccagagttta cagaaaaaat ttttactgtc aagaaaaaaa tagactcgtc
     2401 ggacccccat gatcgggccg tcaagattga ctcggaaaag caatcatgtt atcaacttcc
     2461 ctatatttcg atggagatac gacgggatga ataaagagac gggcagaagg atgatgaacg
     2521 tttttttcag gtccttttgt atccagtcga cgtggaattt ctggagaatg cagaacctgg
     2581 gattcgcttt tgcgatgatc ccttcgattc gcaccctgag caccgacagg gatattaggt
     2641 cgaggatgct tgtgcggcat cttcagatgt tcaataccaa tccttacatg tcaggtccga
     2701 tcatcgggtc cgccatgagg ttagaggaag ccctgtgcag cgacggaaac aaccccgaag
     2761 tggatcagtt gaaggcggcg ctcatggcgc cctatgcggc cataggagat tccttcttct
     2821 ggggtgctct gaggccgctc gcggctgcgg tcggcgtact gcttgcgtta aaaggatttt
     2881 ttatcgcgtt cctcgcaccg atccttattt tcaacccttt taatctctgg gttcgtctga
     2941 aaggtctggt cgaaggttac cgaaggggca aagagggtat cgaattcata cgttcgatga
     3001 acctccttgg gatggcgcgg cagctccggt gggtgtctgt cgtctttctc gccgccctgg
     3061 ctctggtcgt ctcgagccag acggaatggc cctttgtgca gggtctcgat atcctgaaga
     3121 gggtttttct tctggcggtg atccttgctt gcctctgggc tttaaaaaaa ggggtatccg
     3181 ccgtgaccat cctctacagt gtatctttcc tcctttttct tgtgatcggc ctgagatgac
     3241 gacgagcctg agaatcttca ccatcaccaa tgaactcggt atccatgccc gttcggccgc
     3301 cttgatcgtg gatgtcgcca gtaggttcag gtcggaaata ttgatcgaaa aagacggcag
     3361 ggaggtcaac gcgaagagca tcctcggcat cctaacgctc ggcgctccca aggggagcaa
     3421 gattacggtc agggcggagg ggcccgacgc tcaggaggcc gtcgacagtc ttgggcatct
     3481 catcgaaaat aaatttgggg aaagttgatg ggtgaaagag acagtcagag aaagacgcat
     3541 gttttgaagg ggataggtgt gtcaccggga gtggcgaccg gcaaggcgtt gattctcgac
     3601 tacagcattg ccaatgttcc ctgttatcag cttgggagtg aggcggaaac gctggtagag
     3661 atttccaggt ttgagaaggc attgaaggaa tcgaggcgtc agctagcggt gctccggaag
     3721 aaactctccg accgggaggg gccgaaaccg cttttcatca tggatgtgca catgatgatg
     3781 ctcaaagaca agacactgat aaacaacacg gtcaacgtca tcaaggagag gatgatcaac
     3841 gccgagtggg ccctgatgct cactatagat aagtaccgcg agatattcga gaagatcgac
     3901 gacgaatact tgagggagcg tttcagtgat gtggaatacg tgggccagag gatactcagg
     3961 aacttagtcg gcagggagca catcaggctt gcggacatcg aagaagcgtc gatcatcatc
     4021 tctaaggatc tgtcgccggc cgacacggtt cagatgaaag tcgaaaacat cctcggcttt
     4081 gcgaccgata tgggaggaaa gacctcgcac accgccatcg tcgcaaggtc gatcggtatc
     4141 ccggctgtcg tggggttaga gcgggtcacg cgggaagtca gacctggcga catcgtggtc
     4201 gtagatgggc tggacggttt cgtcatcgtc aaccccgacc cggaactcat caggcgctat
     4261 gaggaaaaga aaaggcattt tgagatactc gaggagggtc tgctgcggga ggcgaggctg
     4321 ccggcggtca caagggacaa ccgaaaggtc gatatatcgg cgaacataga attcgtagaa
     4381 gagatcccct ccgctgtggc ccatggagca gagggtgtag ggctttaccg taccgagttc
     4441 atatacataa accgggaaca actgccgacg gaggatgagc atttcgtcaa ttacaggagt
     4501 gtgatcgagg gccagggcat aaagtggtct acaatcagga ccttcgacct gggtggtgat
     4561 aaattcattt ccgacccaaa gctggccaaa gagatgaacc cggtcatggg attgcgggcg
     4621 atccggttct gcctgcaaga aatagaactt ttcaaggttc agctgagggc gatcctgagg
     4681 gccagcgctt acgggaagac acgggtgctg ttccccatga tttcctcgct ggaggagatt
     4741 atcgaaacca agaagatatt cggggaggtc aaggaagacc tcatcaggcg gggggttccc
     4801 gtcggcgagg acatcgaatt ggggatcatg atcgaggtac cgtccgcggt cataatcgcc
     4861 gacagccttg cccgtgaagt cgacttcttc agcatcggca ccaacgattt gactcagtac
     4921 acccttgcga tcgaccgggt caatgagcgg gtcacctatc tttacgaacc cttgcatccg
     4981 gccgtcctga aactgatcaa gatgatcgta gatgcaggcc accgggcggg gatcagagtt
     5041 tccatgtgtg gagaaatggc gggagagccg acctacaccc ttgtcctctt aggcttcgag
     5101 ttggacgagc tgagtatgaa ccccttggcc ataccgcggg tgaaaaagat catccgcaac
     5161 gcaaccttcg aggaatcgca ggccctgttg aacaaggtta tgacgttttc cacggctcgg
     5221 gagatagagg cctacgtaag ggactacatg acgggccgtt ttccggagga tttcagacag
     5281 gaagactgac aggcgcatca gaccatgacg acaaacgtcg catcagagaa aaaggccgga
     5341 aagaaagagt acggttgcta cggaggctcc ctttacgact cgagtggttt ttccctcagc
     5401 cgttttctgg aaaggattct ttcccgggtg ggattttccg atgaggccgc ggaggaattg
     5461 aggagccttc accaaagggg tgtcgtggtc tatgcccaaa agaatgcgag tcagcttgat
     5521 gccctgatcc tgagagaggt tctcaaaaaa aagggaatcc cgcccccgat ctattgccat
     5581 gccgtcaaca tggtggcctg gcaaccgtta tttacagccc ttaagtgcgc ttttacgcgc
     5641 atcttcaacc tggtgttcaa gaaagacagc ctcaatcctt acaagactga atacctgaag
     5701 gaaataacga aaaaaggcgc cagctccctc atctacctac gggactcgga ttttttggga
     5761 agtccctacc ccaaggatcc gctcatccag cttatccacg cccagaagga gatggaaacc
     5821 cccgtcttca tcgtgcccgt cggttttttc tacggcaaga ggagggagaa gaagaacaag
     5881 accatcatgg aaatactcct cggccatgta gagaaccccg acccgctacg gagggtcatc
     5941 acctttctcc ggtattcgaa gaaggcgacc gtggttcctg caatgcccgt caaccttacc
     6001 gaatacttag aatctgctaa cccgccgcgt tcctacgaaa gtatttccta caacctgcgc
     6061 agagaactga tcgaccggat caacgaggaa cgacgcgccg tcttcggccc tgtactcaag
     6121 tcgcgagagg agatcatcgt cacggtgctg cgggacgcct atcttgtcaa ggcgatggag
     6181 gagctggcga cggcgacgaa gaaggatttc aaagctgtct tcaaggaggc aaagaaatat
     6241 ctgaaggaga tcgccgcgga ttatcaggat atctacatcg acatctggta caagattctc
     6301 acctggctct ggaacaacat ctacgacggg gtggttgtag acagagaagg ccttgcgatg
     6361 attcgtgata tctcaaagcg catgcccttc gtcatcgtgc cgtgtcacag gagtcacatc
     6421 gactacctcc tgttgtccta tgtcttcgag gtcaataaca tccctctacc ctttgttgcg
     6481 gcggggacca accttatgtt ttggcccctc gggccgatct tccggaagtc cggcgccttc
     6541 ttcgtccggc gcggcttcaa ggggaacgtc ctgtaccgcc aggtgtttgc gaagtacatc
     6601 aaagttctcc tgaaagaggg ccttccgatc gagttcttca tcgaaggggg caggagccgg
     6661 accggaaaga tggttatgcc gagatacggc atgctgtcca tggtcatcca ggcatacaag
     6721 gaaggcatca gcagcgacct cgccgtcatc cccgtcttta tcggctatga ccgggtgatg
     6781 gaggaaaagg cctatcttga agagctcggc ggcgcgcaga agacaaggga aaaggcgacg
     6841 gacgtcatca agagcggagg tctcctgagg agacgttacg gtcgtgtgta tgtcaacatc
     6901 gggaaaccga tctttctcaa gagttacatg gcctccctgg agaaaacata cgaagacatg
     6961 actgtcgagg aacggcagag tttttacaga aagatcggct acgaggtcgt cggcgagatc
     7021 aacaaggttt cggtggttac tccctccgca ctcgttgcgg cggcgatgct gtgtcattac
     7081 cggcggggca tttcccacga cgaactcgtg ggcataatca atgagcttta tgaatatctc
     7141 gcctaccgga acgtgaagtt cgcctccacc ttcgccaacc gtgaacgggc ccttgcggat
     7201 gccctgagcc tcttcgagac cgcgggcttg atttccaaga tgggcatcga ggaagaggat
     7261 aggaatgagg aggtcgaaga gatcgtctat tctctggacg aggaaaagcg gctcaaccta
     7321 gagtattaca agaacaacat actccacttc ttcgtccccc tcagcttcgt cgcaacatcc
     7381 atactgtcct ccagtgagga cgcaattctt ttttatcgga tacttgagga ttacaagttt
     7441 ttcagaaggc tcttcaagaa tgagttcatt ttcgacgaca aagtagacga cgtcgaggag
     7501 gtgaatcagg tcctcaatta cagtcacgac cgcggcatga tcgagggaaa ggaacgggac
     7561 aacgaggcct ggatcgaggt caagggtaag gggagggtgg gcctaaggcc ctatgcgggc
     7621 cttatccata attacatcga gtcgtactgg gtggtgatac gcggatgttc gtacctgaaa
     7681 aagggtgctc ggacggagag ggaattcacc aaaaaaatac agcagctcgg caccaggatg
     7741 tataaaaaag gggagatcat gagggcggag gcgttgtcga gctcgaacta caagaacgcc
     7801 gtcaaatttc tctgcgacat ggagataatc tttctccgaa gggagaagga caaaaaggac
     7861 aggaaggaaa ccgttgtcta ttccctgacg gacgaccgca gcgaagtcga gtctctgagg
     7921 cagcggctgt tcaagttcct ctgagcgaac gcgtatgaat cttgtccgtc gtggtttcga
     7981 ccttgaatca ggaactacgg tgtagggatt ctaaacctca tccgggtacc tttggcgcgc
     8041 ctggcggatg gagtccaggc aggagaaaag gatgccgacc aactctgggt cgaactgcct
     8101 tcccgatgcc ttttcgattg cctccaatgc ctccgattcg ccccaggcat gtttgtagga
     8161 gcgtctcgca gtgagggcat cgaagacgtc ggcgatggca acgatccttc cgaaaagggg
     8221 gatctcttct cctttcttgc ccagcggtaa gccgtcgggg ccggcttttc cttgaagggg
     8281 gagg
//
LOCUS       DAOTMX010000031         8227 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_7793_length_8227_cov_3.609398, whole genome shotgun sequence.
ACCESSION   DAOTMX010000031 DAOTMX010000000
VERSION     DAOTMX010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 8227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8227
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7793_length_8227_cov_3.609398"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="P5573_01650"
     CDS             complement(<1..756)
                     /locus_tag="P5573_01650"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HRT26800.1"
                     /translation="MRLKYGLDERPPAKEMALFGLQWFAVTIPVIVTIGKITAGFHFA
                     APEAEVAYLQRLSFVMAVALFTQVVAGHRLPLIIGPSTVLLIGIISSREYGLGPVYTS
                     IAIGGLLLALLSITGLFARLKSLFTTRVVAVVLLLIAFTLTPTVLNLISSPRVGSGPS
                     VNLLFSAGLITAMFLFYRYSSGIWKSTFIIWTMAGGSLFYFMLFPGTFTSTQVVRSAP
                     LVSWALQDIPVGFTVDPGVLVSFIFCFLALAVND"
     gene            903..1337
                     /locus_tag="P5573_01655"
     CDS             903..1337
                     /locus_tag="P5573_01655"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HRT26801.1"
                     /translation="MEKSIKGTKTEKNLMVAFASESMAVIRYALFAEVAGREGYEKAA
                     SAFRETAKDEKTHARIFMRLFEGGMTEITAGFPAGPAGDTKKNLEAAIDGEGKSWRVR
                     YAEFAKTAREEGLEDIAVIFEKIAADERRHEENFHRIREGLE"
     gene            1463..1669
                     /locus_tag="P5573_01660"
     CDS             1463..1669
                     /locus_tag="P5573_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT26802.1"
                     /translation="MQGEELCEICGVKKTFTGCPGCERLMCDTCARFELFGWGCGCVW
                     PVYFCPDCALNPQINPNAPFRDRG"
     gene            1772..2209
                     /locus_tag="P5573_01665"
     CDS             1772..2209
                     /locus_tag="P5573_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT26803.1"
                     /translation="MKVFGAGGGVEEEKRALIERLKTVKDPFERERILWTLEGMKKGV
                     PETRSSREAGKEPPAAEEKVRLPVKLPKGFGSLTQYAAPVVFIFFGLAFILQAVLRGL
                     QKKDFASETGQFVGGAIFLLAGFASLQKAKKTGRVAGGPGERA"
     gene            complement(2214..4955)
                     /locus_tag="P5573_01670"
     CDS             complement(2214..4955)
                     /locus_tag="P5573_01670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRT26804.1"
                     /translation="MFEDISDGYFECDLQGNITFCNSVLSNLLGRSREELSGSSLHSY
                     LDRADAEIVMGGFRAFLEGTLAAPVITYEMARKDGARVPVETSLSLIRDREGRVEGFR
                     GIVRDITERVRREEAQKNELKRYIDEINDLYQNVPCGCHSLDENGVFLRVNDTELGWL
                     GYSREEVVGKKWTDFLTEEGVKTYHETFPVLKERGWIKDLEFDLVHKDGTTMPVLLSA
                     TTIRDEKGRFIATRSTLCDIAKRKQDEAELKEPRRRLSDIIEFLPNATLAVDQDGLVI
                     GWNRAMEALTGVKAEEMLGKGDYEYALPFYGERRPILVDLALHPELAHDHHYTSFQRV
                     GAVVFGEAYAPGLPQENTHISATAAVLRDSRGEVIAAVAFVRDNTEHRKIEEALAAEH
                     ERLTLILDGIPIPAFVIDPDGVVTLWNRNNEIFTGKSKDKVLGEKLDLSFLFEEKTQP
                     ALADLLIGLTDEEITQKFGPDRVRKSNLFPDAFECESQVLVRGERRLILTQASRIVDS
                     RGEVVGVIQTGQDITALVKAQEEGKKLQAQLIQAQKMEAVGTLAGGIAHDFNNLLMGI
                     QGYTSLMMMELDVFHPHYEKLKAVEEQVAAGANLTKQLLGFARRGKYEVKTLDLNDNL
                     YKSSTLFGRTKKEVVIHRKFQGDLWPVEADPGQIEQVFMNLLVNAWQAMPGGGHIYLE
                     TSNLAIREGDEWPPYIKAGRYVKVSVTDTGVGMDEWTRERIFDPFFTTKEMGRGTGLG
                     LAMVYGIVKGHDGYIDVSSEPGRGSTFTVYLPASDKQLVFEGGEDKALLRGTETILLV
                     DDEAVITEVMKDMLELLGYRVFIAGSGREALRIYEENKDVIDLVILDMIMHDMGGGET
                     FDRLREADPGVRVILSSGYSLDGEPAGIMLRGCQGFIQKPVTIAELSQKIREVLAGS"
     gene            complement(5136..5519)
                     /locus_tag="P5573_01675"
     CDS             complement(5136..5519)
                     /locus_tag="P5573_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HRT26805.1"
                     /translation="MKTNARFEMVDITRRVGAVVRESKVTSGVCHVFVPHTTAAVTIN
                     ENADPSVPRDILAELDKVIPLNDNYRHMEGNAAAHIKASLVGPSETVFVDNGELVLGT
                     WQSLFFCEFDGPRTRRVLVEVVGAE"
     gene            complement(5542..6597)
                     /locus_tag="P5573_01680"
                     /pseudo
     CDS             complement(5542..6597)
                     /locus_tag="P5573_01680"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
     gene            complement(6491..7237)
                     /locus_tag="P5573_01685"
     CDS             complement(6491..7237)
                     /locus_tag="P5573_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HRT26806.1"
                     /translation="MMKKAFLAALAVLMLWCSSVSAENYTVEGEMGSQIRFEIRKSVT
                     TVAGVTKLTLSFVVPPTFQSLTYNQEIQGFDIKLSPEPQEKKKTVNERGHEVVTATWV
                     KPPQGIDVRLSFTALNNTHLKPIDTGVPFPVRDVPEDVKYYLKATEQVQAEDPRIVKR
                     AKELTRGVTTEFDAVQRILTYVIDNVRYVTPPAKCDALYALEAKRQLPEFFPPCSRPD
                     AGRRDPDEGRERHHPQQALRPEAQGRSHHL"
     gene            complement(7331..>8227)
                     /locus_tag="P5573_01690"
     CDS             complement(7331..>8227)
                     /locus_tag="P5573_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HRT26807.1"
                     /translation="HAPESLTHLQDIDPKKIGKVLIARKPLRDLTEKREEKGLFGWTL
                     CLFPTAELARKAGLSLKRYADQVIRACYLDHDDPAREWKSIHRKVAIIKQWLSSLPVK
                     AFHIESDSTDLRVTPGEHRQWLGISGHNIPSFEIFLSPDWRGTEGVYYADQPSFRSGN
                     YIEKVRLTFKKGSVVNVEAEKGLEFAKKQIAMDVGAGRVGEFSLTDRRFSRINHFMAS
                     TLFDENFGGRYGNCHLAVGASYSDTYSGDPSKLTKTMKKALGFNDSALHWDLVNTEKK
                     TVTAHLKTGEKVVIYDGGIFRW"
BASE COUNT         1623 a         2353 c         2446 g         1805 t
ORIGIN      
        1 gtcgttcacc gcgagggcaa gaaaacagaa gataaaagaa accagtacac ccgggtcgac
       61 cgtgaagcct acgggtatgt cttggagggc ccaactgacg aggggagcac tgcggaccac
      121 ctgcgtcgag gtgaaggttc ccggaaagag catgaagtaa aacaggctcc ccccggccat
      181 ggtccagatg atgaaggtgg acttccagat acccgacgag taccgataga agaggaacat
      241 ggctgtgatc aggccggcgg agaagagaag gttgaccgac ggccccgagc cgacccgggg
      301 agagctgatg aggttgagga cggtgggggt cagggtgaag gcgatgagga ggaggaccac
      361 cgcgacgacc ctggtcgtga acaggctctt caaacgggcg aagagccccg tgatactcaa
      421 aagcgcgagc aacaacccgc cgatcgcgat ggaggtgtag acggggccga ggccgtattc
      481 ccgactcgat atgatgccga tgagaaggac ggtggagggt ccgatgatga gcgggaggcg
      541 gtgcccggcg acgacctggg tgaaaagggc gaccgccatc acgaaggaaa gcctctgcag
      601 gtaagctacc tcggcctccg gcgcggcaaa atggaacccc gccgtgatct tgccgatggt
      661 gacgatgacg gggatggtga cggcgaacca ctggaggccg aagagcgcca tctccttcgc
      721 cggcggccgt tcgtcaaggc cgtatttaag tcgcatgttc aggttccgtt gtcaggaagt
      781 gcggcggttg cgcccgtcct tctttaacca atccgtcatt tgttgtaaag gtctttaaaa
      841 aaccccatgg ataggaccga ccttttttgt tagactccga gcaagaaagg ggtgggggta
      901 agatggaaaa atccatcaag ggaacgaaga cggagaagaa cctgatggtt gcctttgcga
      961 gcgagtccat ggctgtgatc cgctacgcct tgtttgccga ggtcgcgggg agggagggtt
     1021 acgaaaaggc cgcttcggcc ttccgggaaa cggcgaaaga tgaaaaaacc catgcgagga
     1081 tattcatgcg gctcttcgag ggaggcatga ccgaaatcac ggccggcttc ccggccggac
     1141 ctgccggtga cacgaagaaa aacctcgagg ccgccatcga cggcgaaggc aaatcctgga
     1201 gggttcgcta cgccgagttc gcaaagacgg ccagggaaga gggcctcgaa gatatcgcgg
     1261 tcatcttcga aaagattgcc gccgacgaaa gacgccatga agaaaacttc caccgcatcc
     1321 gtgaaggtct ggagtaggaa ggcccggcgt caaacgcatt ttttgcattc cccggaaggg
     1381 ctctggtata atccgcggta atgtcagacg ttttatgaaa gcagaagatg gaatacggaa
     1441 aaagctcaga ggaggttgga ggatgcaggg agaggaactg tgcgaaatct gcggcgtcaa
     1501 gaagacattc accggatgtc cggggtgtga gagattgatg tgcgacacct gcgcacgctt
     1561 cgagctgttc ggctgggggt gcggatgcgt gtggcccgtc tatttctgcc ctgactgtgc
     1621 tctcaacccc cagataaacc ccaacgcccc tttccgggac cggggataag gcaggagaag
     1681 agagaaacgg cgctacttcc ttgtccttgt cgagagggcg ccgaggagtg ccggtcaaac
     1741 gatgtctggt tacaaccgaa ggaggtaaaa catgaaggta ttcggggccg ggggaggagt
     1801 ggaagaggag aaaagggccc tcatcgagag actgaagacc gtcaaggacc cttttgagag
     1861 agaaaggatc ctctggaccc tcgaaggcat gaagaagggt gtccctgaga caagaagctc
     1921 tcgcgaggcc gggaaagaac ctcctgcggc ggaggaaaaa gtgaggctgc cggtgaagtt
     1981 gccgaagggc ttcgggtctc tgacgcaata cgccgctccc gtcgttttca tcttcttcgg
     2041 tcttgctttc atcctccagg ccgttctcag ggggctgcag aagaaagatt tcgcctccga
     2101 aacgggtcag ttcgtcggcg gcgcgatatt tcttcttgcc ggttttgcct ctctacagaa
     2161 ggctaaaaag acggggagag tcgctggagg ccccggtgag agggcataag cgtctacgag
     2221 ccggcaagaa cttctctgat tttttgcgac agctcggcga tggtgactgg tttttggatg
     2281 aatccctgac agccccgcaa catgatgccc gcaggctcac cgtctaggct gtatccgctc
     2341 gagaggatta ccctcacccc ggggtcggcc tccctgagac ggtcgaaggt ctctcctccc
     2401 cccatgtcgt gcatgatcat gtccaggatg acaaggtcga taacgtcttt gttttcttcg
     2461 taaatcctca acgcctcccg gccgctgccg gcgatgaaga cccggtaacc gaggagctcc
     2521 agcatatcct tcatcacctc ggtgatgacc gcctcgtcat ccaccagcag tatcgtctcc
     2581 gtgccccgca gaagcgcctt gtcctctccg ccctcgaaaa ccagttgttt gtctgaggcg
     2641 ggcaggtaga cggtgaaagt cgacccgcgg cccggttcgc tcgagacgtc gatatagcca
     2701 tcatgtccct tgacgattcc gtagaccatg gcaaggccga gcccggtacc ccgtcccatt
     2761 tccttcgtgg tgaaaaaggg gtcgaagata cgctccctcg tccattcgtc catcccgacc
     2821 ccggtgtctg tgaccgagac cttcacgtac ctgccggcct ttatgtaagg cggccactca
     2881 tcgccctccc ggatagcaag gttcgaggtc tcgaggtaga tgtggccgcc acccggcatg
     2941 gcctgccagg cgttgaccaa aagattcatg aagacctgct ctatctggcc tgggtccgct
     3001 tccacgggcc acaggtcgcc ttggaacttg cgatggatta caacctcttt cttcgtcctg
     3061 ccgaagaggg tggaactttt atagaggttg tcattcaggt cgagtgtctt cacctcatat
     3121 ttgcctctcc ttgcgaaccc gagaagctgt ttggttaagt tggcaccggc cgcgacctgt
     3181 tcctcgaccg ccttgagctt ttcataatgg ggatggaaaa cgtcgagctc catcatcatc
     3241 agggaggtgt aaccctgtat ccccatcagg aggttgttga agtcgtgggc tattccccct
     3301 gccagcgttc ccactgcctc catcttctgc gcttgaatca actgggcctg cagctttttg
     3361 ccctcttcct gcgccttcac cagtgccgtg atgtcctgcc cggtctggat gacaccgacg
     3421 acctctccgc gtgagtccac gatccttgag gcctgggtca gaatcagccg tctttccccc
     3481 cgaaccagga cttggctctc gcactcaaag gcatcgggga ataggttact cttacggacc
     3541 ctgtcggggc cgaatttttg ggttatctcc tcatcggtca gcccgatgag gagatccgcc
     3601 agggccggct gcgtcttttc ctcgaaaaga aaactcaggt ccagcttctc tcctaacact
     3661 ttgtctttgg atttaccggt aaaaatctcg ttattcctgt tccagagcgt gacgacgccg
     3721 tccgggtcga tcacaaaggc gggtatcggg atgccgtcga ggatcaaggt caaccgttcg
     3781 tgttcggcgg ccagcgcttc ctctatcttt ctgtgctccg tgttgtcgcg gacgaatgcg
     3841 acggccgcga tgacctcgcc ccgggaatca cggaggaccg cggccgttgc cgaaatgtgg
     3901 gtattttctt gcgggagccc gggtgcataa gcctccccga aaacgacggc gccgacccgt
     3961 tgaaaactcg tgtaatggtg gtcatgtgcc agctccgggt ggagggccag gtcgaccagg
     4021 atcggccgcc gctcgccgta gaatggcagg gcgtattcat agtcgccctt tccgagcatc
     4081 tcttcggcct tgacgcccgt gagcgcttcc atcgcccggt tccaccctat caccaggccg
     4141 tcttggtcga cggctaaggt ggcgttgggc aggaattcga tgatgtcgga gagccttctc
     4201 cgcggttcct tgagttcggc ctcgtcctgt tttcgcttgg cgatgtcgca gagggtcgac
     4261 cgggtcgcga tgaaacgacc cttttcgtcc cggatggtcg tcgcactcag gaggacgggc
     4321 atcgtcgttc cgtccttgtg gaccaggtcg aattccaggt ctttgatcca gccccgctct
     4381 ttgaggacgg ggaaggtctc gtggtaggtc ttgacgcctt cctccgtcaa aaagtcggtc
     4441 cacttcttcc cgacaacctc ttcccgggaa taccccagcc accccagctc ggtgtcgttg
     4501 acccggagaa agacgccgtt ttcatccagg gaatggcagc cgcagggtac gttttggtaa
     4561 aggtcgttga tctcatcgat gtatcttttc agttcgttct tttgcgcctc ttccctccgc
     4621 acgcgctccg ttatgtccct gacgataccc cgaaaccctt ccacccggcc ttcccggtct
     4681 cggatcagcg acagggatgt ctcgacgggc acccgcgccc cgtcttttcg cgccatctcg
     4741 taggtgataa ccggcgccgc cagggtgcct tcaaggaagg ccctgaagcc tcccatgacg
     4801 atttcggcat cggcccggtc gaggtaagaa tgaaggctgc tccccgacaa ttcctcgcgg
     4861 gaacggccga ggaggttact caaaacggaa ttgcagaaag ttatgttccc ctggaggtcg
     4921 cactcgaaat atccgtcgct tatgtcctcg aacatactcc gattgtcttc gggcttccgc
     4981 tggtgcaggt ttttaggttt cgggcaagac atgttccggc tgtctgctta aatgtcggac
     5041 taaaagagaa aacagggttg taacgaaatc aaggcgctac acaggtcaga aaagctgata
     5101 cattcaaaac aaacccatgg tcgatgtcaa ggcctttatt cagcgcccac tacctcgacg
     5161 agcacccgcc tcgtgcgggg tccgtcgaac tcgcagaaaa agagggactg ccacgtaccc
     5221 agaaccagtt caccgttatc gacgaaaacg gtctcagagg gaccgacgag agaggccttg
     5281 atatgtgccg cggcgttccc ctccatgtgg cggtagttgt cgttgagcgg gatcaccttg
     5341 tccaactcgg ccaggatgtc ccgcggcacg ctggggtcgg cgttttcgtt gatcgtgacg
     5401 gccgccgtag tgtgggggac gaagacgtgg cagacgccgc tcgtgacctt gctctcccgg
     5461 acgacggcac cgacgcggcg ggtgatgtcg accatctcga aacgggcgtt cgttttcaca
     5521 gagaattgtt tcataggaca cctattttgt cgtcatccca atcacacggt agttgaactc
     5581 gtagctcaat gccccgctcc agtcttcttc atagacgccc ttgtaccggg tgccctctac
     5641 cggcccgacg ggcttaccgt aggtgtagaa ccagttcaga acggggctcc ccgtgaagcc
     5701 cagaccgatc cagtaagcgc cgggcatgag cgtgatttcg tccttgaaca caaaatcctg
     5761 ccagcggtaa ccgggccttt ccgagatgtc gtccaggttc accatttcgc ttgtcgtgat
     5821 ctgttcgccg ggtttgtcgt ttgcgtcctt gaagaggtcg acccagatct ggccgtcacc
     5881 gccgaattta tgcagggcaa ggccgacctt gtggagacgg accggtttca ccaggacgaa
     5941 aacctgggca tactgggtga gcttggtggt cacgtattcc gccgtctcga catagaagct
     6001 cctcgttttt tcgaatctcg tcttgccctt cgcgaccgtg ggcgtcggcg ggaaggcaaa
     6061 atcgatgttc tccgggaatt ccaggttccc gaacaggaaa ggcacggtaa agcgcagttc
     6121 ttttttttct tccgctgtga tcacgggcgg cggtggcggg ggcggtacct cgattttctt
     6181 gaaggccgcc ttcacgtgag ggacgagcag aaggttcttg ggcccgtaat cctggcgcct
     6241 gccctccaca ttcacccggt ccgcgttgaa atcgccgttg atcgtctcct gggcgttcgg
     6301 ctccccgtcg ctcccctttc tttgtgtcca gcgcatcagg ccgtcgttga cggtctcgtt
     6361 gttgtccgcc cccgtttcga cccggatgta gcggttggag acgaacatgg ccgtctgctg
     6421 cgggtcgaag gggacccagc cgaggtcggg aaaccagacc tcgatccacg aatggcggcc
     6481 ctgtcccatc ttaaaggtga tgactccgcc cttgcgcttc aggtcgaagg gcttgttgag
     6541 ggtgatgccg ttcacgaccc tcgtcgggat cccttcggcc cgcatcaggg cggctgcaag
     6601 gtgggaaaaa ttctggcagt tgcctcttcg cctcgagcgc gtacagggcg tcgcactttg
     6661 cgggcggcgt cacgtaacgg acgttgtcga tcacgtaagt gagtatccgc tgtacggcgt
     6721 cgaattcggt cgtcactccc cgggtcagtt ctttcgcccg tttcacgatg cgggggtctt
     6781 cggcctggac ttgttccgtc gctttcaggt agtacttgac gtcctccggt acatcccgga
     6841 caggaaaggg aacgcccgtg tcaatcggct tcaggtgggt gttgttcagg gcggtaaagg
     6901 aaaggcggac gtcaatgccc tgcggcggct ttacccatgt cgccgtaacg acttcatggc
     6961 cccgctcgtt gactgtcttc tttttctctt gcggttccgg agacaatttg atgtcgaacc
     7021 cctggatctc ctggttgtac gtcagggact gaaaggtcgg ggggacgacg aagctcaacg
     7081 tgagcttggt cacgcccgcg acggtcgtca cgctctttcg tatctcgaac cgtatctgcg
     7141 accccatctc gccttcaacg gtgtagtttt ccgccgaaac ggagctgcac cagagcatga
     7201 gtacggcaag agcggcgaga aacgcttttt tcatcattcg acgtctccta cgataccttc
     7261 atcccccgcg aaagtccttt cccataaaaa ggaagatggc gtgggcaaag gggatgaacc
     7321 gccgtgactg tcaccatctg aaaattcccc cgtcgtagat gacgaccttt tcccccgttt
     7381 tcaggtgtgc ggtgaccgtc ttcttttccg tatttacgag gtcccagtgg agggccgagt
     7441 cgttgaaccc gagggccttc ttcatcgtct tcgtaagctt cgaggggtcg ccgctgtaag
     7501 tgtcggagta cgaggccccg acggcgaggt ggcagttgcc gtaacggccc ccgaagtttt
     7561 cgtcgaaaag ggtgctcgcc atgaagtggt tgatccggga aaaccgcctg tccgtgagcg
     7621 agaactcgcc caccctcccc gcccccacgt ccatggcgat ctgttttttc gcgaattcca
     7681 gtcccttttc cgcctcgaca ttgacgacgg accctttctt gaaggtcagc cgcacctttt
     7741 ctatgtagtt gccgctccgg aaggacggct ggtctgcgta gtacacccct tccgtgccgc
     7801 gccagtccgg tgagaggaat atctcgaagc tggggatgtt gtggcctgag atccccagcc
     7861 actgccgatg ttcccccggc gtgacacgga ggtccgtgct gtccgactct atgtggaagg
     7921 ccttgaccgg caggctgctc aaccattgtt tgatgatggc caccttccgg tgaatcgact
     7981 tccattccct ggccgggtcg tcgtgatcca ggtagcaggc ccggatgacc tggtcggcat
     8041 accgcttaag ggagagtccc gccttccggg ctagttccgc cgtcgggaag aggcagagcg
     8101 tccacccgaa gaggcctttc tcttccctct tctcggtcag gtcccgcaag ggcttccggg
     8161 cgatgaggac tttgccgatt ttcttggggt cgatgtcctg aaggtgtgtg agcgattcgg
     8221 gggcatg
//
LOCUS       DAOTMX010000032         8120 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_7920_length_8120_cov_4.008308, whole genome shotgun sequence.
ACCESSION   DAOTMX010000032 DAOTMX010000000
VERSION     DAOTMX010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 8120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8120
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7920_length_8120_cov_4.008308"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..367)
                     /gene="groES"
                     /locus_tag="P5573_01695"
     CDS             complement(77..367)
                     /gene="groES"
                     /locus_tag="P5573_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HRT26808.1"
                     /translation="MKIRPLHDRIIVKRVEEEAKTKGGIIIPDTAKEKPIEGRVIAVG
                     KGRVADDGKVIPMEVKAGDHILFSKYAGTEVKIEGEEHLIMREEDVLGIIEK"
     gene            623..1243
                     /locus_tag="P5573_01700"
     CDS             623..1243
                     /locus_tag="P5573_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HRT26809.1"
                     /translation="MKFLPKEETFFDHFEELAVKIEEGAKLFIDILNNFEHSEAKVTT
                     LKDVEHEADTITHRIYEKMHKTFLTPFDREDIYALANKMDSILDMIEATAIRMYLYKV
                     DNPNNEIKEMARTLSNTVAKVKEIVYGLRDKKKSKMILEACVVINTLENEGDYIMRQA
                     MARLFDRETNAKELIKWKEIFERVEEAIDVCEDVSNIVEGIVLKHG"
     gene            1236..2237
                     /locus_tag="P5573_01705"
     CDS             1236..2237
                     /locus_tag="P5573_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HRT26810.1"
                     /translation="MGDSSLVAVICVVLIALVFDFINGFHDAANSIATVVSTRVLKPR
                     QAVLFAAFFNFIAVFFIGVAVAKTVGTGIIDPSIIDNRLILSALAGAIAWNLITWWVG
                     LPSSSSHALIGGLIGAAIIRGGTAALIWEGIIKTASFIVLSPSIGLILGLLMMVLVLN
                     FSGKTTVSKADRIFRRLQLFSAAVYSLGHGMNDAQKTMGIIAIVLFNNGFLGPTFHIP
                     YWVEVACYTVIALGTMTGGWRIVKTMGTKITKLQPMGGFCAETAAAVAIIGASVAGIP
                     VSTTHTITGAIMGVGSTKRLTAVRWGVAGNILWAWVLTIPVSAMMSAAIYFATKALF"
     gene            2410..2985
                     /locus_tag="P5573_01710"
     CDS             2410..2985
                     /locus_tag="P5573_01710"
                     /inference="COORDINATES: protein motif:HMM:NF028046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT26811.1"
                     /translation="MKRLTFLKVFASYLILILITAAVLDFFLTQQIKDIMAKGMEDEL
                     FSIARITAFIPGERIQSDIPEIAKQLNVRLTLIDTSGLVLADSQADASKMDNHLNRPE
                     IQQAVKEGKGKSSRFSVTIQESMLYVALPVKENSRIKGYIRLARPMTAVTKSIDILYR
                     AIHLTLYIIAIPSLILALIFSRYMAARLTDD"
     gene            complement(3125..4180)
                     /locus_tag="P5573_01715"
     CDS             complement(3125..4180)
                     /locus_tag="P5573_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT26812.1"
                     /translation="MINYIFLITLTLLFALLFGWCFKHLPEERWQILASVPVKKISPG
                     VWEGMNVTYYGLFNANAIVIAVSIFFILTGAARVPLSVTLATVFVILLLCLPSSRILA
                     RLIEKKAYTSTVGGASFVGLLVAPLVLWAMDGARRMIAADSFESVSILAAIAVAYAFG
                     EGLGRLACISFGCCYGKPLSRCGRVLQAVFRHCSFVFSGKTKKIAYESGLDGQKVIPI
                     QAVTAVIYVGVGLVGTCLFLQARFTSAFLIAVLGVQVWRVVSETLRADWRGRGNLLTA
                     YQVMSLGGAAYAVFIAAVMPVGHDLIVSVVDGLRSFLQPGTIIFLTLLWLFCFFYTGR
                     SRVTGSQVTVYVHSSRI"
     gene            complement(4173..5006)
                     /locus_tag="P5573_01720"
     CDS             complement(4173..5006)
                     /locus_tag="P5573_01720"
                     /inference="COORDINATES: protein motif:HMM:NF014696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HRT26813.1"
                     /translation="MNACGIDMTESLECPDRLDTLEKIFQRKIRYWECRPMPEEPGTV
                     VSPADARVLLGSFCETSSLFVKGKFFNYEELLGRDKTDWLAAFWDGDFAVFRLTPDKY
                     HYNHTPVAGRAVDFYEIQGEYHSCNPTAVISVVTPYSKNKRVVTVIDTDIPGGTGVGL
                     VAMVEVAALMVGQIVQCYSKERYDAPVPTCRGLFMEKGMPKSLFRPGSSTDILIFQKD
                     RMRFSEDLLCNLRCPNAESRFDVGFGQHLVETDVKVRSLIGRAVNILSSQNRNTINCH
                     D"
     gene            complement(5182..7656)
                     /locus_tag="P5573_01725"
     CDS             complement(5182..7656)
                     /locus_tag="P5573_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT26814.1"
                     /translation="MSRLHTVTECLERRAASPSNDPEFLKKIIELMGIDTRSVGALVE
                     TLPFSGSDATAGTETELQAAVIGRRDNVDLPIMIEQSNYYANIMRRTAAGDTPRKVIT
                     DLERWLAANRDNVWENSWVRFPLAVLSSFAKDVFRNDLLSDKQSRNNTLRSDAHKFVV
                     SAEGEEYLRVPLSYLLKLALADVVSAGADVPRPVHRAGCELMEHFLSDNTSPETHSFW
                     LVFSGKGSDAGKGIAGETLIRFLLTQLLIMYANRKFCLAGNGQKAVIFSSPHPPVRQK
                     WLNDCVSDAFYRELFMNPCLSGWDRGELKLEYMHLCHGVLSRSQLNAVAKLKEAGILT
                     RNLVVLPNLSNISLANNGTHISLGSRRLSKLLSDPSSGFTRIHEKYVGDLVIKITEHF
                     LPLFVGTYSAAPYRFDFEDFHPEKALGFLPHELDYTHLRMFWRRWKKKAHVGVMGRPL
                     TPFGIPRLDRIISGLFGLRGDYVPDLRLIDYPVMLMSTEKSPALDGKIGNSDRLKKDL
                     SDLGIFDAHMSLYILYKLREYEVMGFSGFEGRHYSLFENYEEDMGRAAQLQILITALA
                     YKYIAMGLCTHAQIPDDPFVESERRQVIFGTAAGIPTFFVRSDTRNLFMQKILLRVYR
                     MRRSRRYPGYLRVHNEEYQRALVRIIEEDAADLIDILCMKETIKDLVSRLDHPDEFST
                     AGRLTRGIVEEANTNAPMKLSAKEFNSAAERYYRERLRKRHLTEALQCLEKGLRKIER
                     CSDDADSASILALRATLGNRRLQDFLRPIEEDVVEDRVSLGDLVKLIHLSLIHTYAER
                     RKAESLSKSICDDEDRTSPIPGSGIR"
     gene            complement(7713..>8120)
                     /locus_tag="P5573_01730"
     CDS             complement(7713..>8120)
                     /locus_tag="P5573_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT26815.1"
                     /translation="VDELKQEGFSKLTKAFPQSIIQVPIDCLVPLILSVPGEALALLD
                     KFRSWGVNRFSMSWKSSNIHRVLDKLDKWGFEANIYNVPDLESFLQAVLLLPTSVTSD
                     FNFPKWHYYGRGSGENQKRHSYEIVKTTQSLSL"
BASE COUNT         2017 a         2200 c         1950 g         1953 t
ORIGIN      
        1 aatagacttt cttgcctctt catcgtactg aattattttg ggtgccatct atttcatcct
       61 ccttttgctc gtctgtttac ttttcgatta tgccgagaac atcctcttcc ctcatgataa
      121 gatgctcttc gccctcgatc ttgacctccg tcccggcata cttactgaaa agtatgtggt
      181 cccccgcctt tacctccatc gggatgacct tgccgtcatc ggccactctt cccttgccca
      241 cagcgataac cctgccctcg atgggctttt ccttggccgt atcggggatt atgatccctc
      301 cttttgtctt cgcctcttcc tccacgcgct taacgatgat tctgtcgtgc aacggtctga
      361 ttttcattcg ttatctcctc ctcctttcaa ctatgctgag ccttaaaaat tccttatcct
      421 tcccggaaaa atcaggcata aaataaacat caatttttca gtgtcaaggg ttttctgaaa
      481 tttttattga aattgggctt aataaaaggt agaagcccaa accaaacaag gcgcaccttc
      541 tgcttcatcc tcaaagacat cgacccgggg gacacgaccg gggtgtcgaa tatctcgccg
      601 atagagcaag gagagccaac ccatgaaatt tcttccgaaa gaagagacgt ttttcgatca
      661 cttcgaggaa ttggccgtca agatcgaaga gggcgcaaag cttttcattg atatactgaa
      721 caattttgag cactcggaag ccaaggtgac cacgctcaag gacgtcgagc acgaagcgga
      781 taccataacc caccgcatat atgaaaaaat gcacaagacg ttcctcactc ccttcgaccg
      841 ggaagacatc tacgccctgg ccaacaagat ggacagcatc ctcgacatga tcgaagccac
      901 cgccatcagg atgtaccttt acaaggtaga taatcccaac aatgaaatca aggaaatggc
      961 acgcaccctg agtaataccg tcgccaaggt caaggaaatc gtttatggct taagggacaa
     1021 gaaaaaatcg aagatgatcc tggaggcctg cgtggtaatc aacaccttgg agaacgaagg
     1081 ggattacatc atgcggcagg ccatggcacg ccttttcgac agagagacga acgccaaaga
     1141 acttataaaa tggaaagaaa tctttgagcg ggtcgaggaa gccatcgatg tctgcgagga
     1201 tgtctcgaac atcgtagagg gaatagtcct gaagcatggg tgattcctcc ttagtcgctg
     1261 ttatttgcgt cgttcttatt gcccttgttt tcgatttcat caatggattc catgacgcag
     1321 cgaattcgat cgctaccgtc gtatccaccc gcgttctcaa gcccaggcag gccgttctct
     1381 tcgcagcctt tttcaacttt atcgccgtgt ttttcatagg tgtcgcggtc gccaagacag
     1441 tcggaaccgg catcattgac ccctccatca tagacaaccg actaatccta tccgccctcg
     1501 ccggcgcgat tgcatggaat ctgatcacat ggtgggtggg cctgccgagc agttcttccc
     1561 acgccctgat cggcggcctg atcggagccg ccataataag gggcggcaca gccgccctca
     1621 tctgggaggg catcatcaag acggcgagct tcatcgtcct ttcgccgtcc atcggcctca
     1681 tcctcgggtt actcatgatg gtcctcgtcc tgaatttttc aggtaaaaca acggtgagca
     1741 aggcagaccg gatattccgg cgattgcagc tcttttccgc cgccgtgtac agtctgggcc
     1801 acgggatgaa cgacgcccag aagaccatgg ggattatcgc gatcgtcctc ttcaataacg
     1861 gttttttggg accgactttt cacattcctt actgggttga ggttgcatgc tacacggtca
     1921 tagccttagg aaccatgacg gggggatggc gcatcgtaaa aaccatgggc acaaaaatca
     1981 cgaaacttca gcccatgggc ggcttctgcg cggagacagc cgcagcggtc gccatcatag
     2041 gggcttccgt tgcaggcatc ccggtgagca ctacccacac cataaccggc gctatcatgg
     2101 gcgtcgggtc cacaaaacgc ctgacggccg tgcgttgggg cgttgccggg aatatactct
     2161 gggcctgggt cctgacgata ccggtctcgg ctatgatgtc ggcggccatc tattttgcca
     2221 caaaggctct attttaatca cagtgcatct gatcacgact ttccccgaaa agtactttct
     2281 tttttcccat attttgattt atctgtcctt ccgagacatg ttgttatata ctttctaccc
     2341 gcttcgcaaa tagcacaatg caaaatcatt gcgtgcggcg agcctgatta ttgaggaggt
     2401 cgttcaacca tgaaacgtct taccttctta aaagtgttcg cttcctacct gatattgatc
     2461 ctcattactg cagccgttct ggattttttc ctcacgcagc agatcaagga cattatggca
     2521 aagggtatgg aggacgagtt attcagtatc gcccgaatca cggccttcat tcccggggaa
     2581 cgcattcaaa gtgacattcc ggagatagcc aaacagttga atgttcggtt gactctcata
     2641 gatacttccg gactggttct ggccgattcc caggccgatg cgtcaaaaat ggacaatcat
     2701 ctaaaccggc ccgaaatcca gcaggccgtc aaggaaggaa aaggcaagtc gtcgcgcttc
     2761 agtgtgacga tccaagaaag tatgctgtat gtagctctcc cggttaaaga aaattcccgc
     2821 atcaagggtt atattcgatt ggcacgtccg atgaccgcag tgacgaaatc gatagacatt
     2881 ctatatcggg cgatacatct aacgttgtac atcatcgcga tcccctccct tatactcgcg
     2941 cttatctttt caagatacat ggcggcccga ttgacagacg attgactttc agatcacctt
     3001 cactgaagaa ctgagggcaa aaaacgttct ggtttcttca aggatcagcc atctttttgc
     3061 acatctatca tgattgcccg accgggaagg aacggaaatt cacctgccca acaggccgtc
     3121 gctttcagat ccggctagaa tgcacgtaaa ctgtcacctg ggatcccgtc acccggctgc
     3181 gccccgtata gaaaaaacag aataaccaca ggagagtcag aaaaataatc gtgccgggtt
     3241 gcaggaaaga tcgcagcccg tctactacgc tcacgatgag gtcgtgtccc acaggcataa
     3301 ccgctgcgat gaaaacggca tacgcggcgc ccccgaggct cataacctga taagctgtca
     3361 acaaattccc tctaccccgc caatccgctc tcagcgtctc ggatacaacc cgccagacct
     3421 gcacccccag aaccgctatc aggaaggcag atgtgaaacg agcctgcagg aaaagacagg
     3481 ttccgacaag tcctacccct acataaataa cggctgtaac agcctgaata ggaataactt
     3541 tctgaccgtc taggccgctt tcgtaggcaa ttttcttcgt tttgccagag aagacaaaac
     3601 tgcaatgtct gaacaccgct tgaagtacac ggccgcatcg agaaaggggt tttccgtaac
     3661 agcaaccgaa acttatgcag gccagccgcc ccaacccttc tccaaacgca tacgcaaccg
     3721 caatcgccgc caggatcgac acggattcaa acgaatccgc cgcaatcatt ctccgggcgc
     3781 cgtccatggc ccatagaacc aagggggcaa ccagaaggcc aacgaacgat gcgccgccga
     3841 cagtcgatgt gtaagccttt ttctcgatca gccgggcaag aatcctcgat gacggcaagc
     3901 ataaaaggag gatgacgaaa acagtcgcta aagtcacgga gagcggcacc cgggcagctc
     3961 cggtcagaat aaagaagatg gatacggcga taacaatcgc atttgcgtta aacaaaccgt
     4021 aataagtcac attcatgcct tcccatacgc cgggcgaaat tttcttaacc ggaacggatg
     4081 ccaggatctg ccatcgctcc tcgggcaaat gtttaaaaca ccaaccaaag agtaaggcaa
     4141 acagaagcgt gagtgtaatc agaaaaatgt agttaatcat ggcagttaat tgtgttacgg
     4201 ttttgcgagg atagaatgtt taccgctcgt ccgatcaatg acctgacctt tacgtccgtt
     4261 tccacaagat gttgaccgaa tcctacgtca aaacggctct cggcattggg acagcgcaag
     4321 ttgcaaagga ggtcttcaga aaaacgcatg cggtccttct ggaatatcag aatgtccgtg
     4381 ctgctgcctg gacgaaaaag gcttttcggc atccctttct ccataaaaag gccccggcaa
     4441 gtcggcaccg gggcatcata tctttctttg ctgtagcact ggacgatctg gccgaccatc
     4501 agggccgcga cctctaccat ggccacaagc cccacaccgg ttccacccgg aatgtcggta
     4561 tcgatgaccg tgaccactcg tttgttctta gagtaaggtg taaccacact gatgactgca
     4621 gtgggattgc aagagtgata ctctccttgg atttcataga aatcaacggc tctaccggcc
     4681 acgggcgtat ggttgtaatg atatttgtcg ggcgtcagcc tgaagacggc aaaatctcca
     4741 tcccaaaagg cagccaacca gtccgtttta tcccggccca ataattcctc ataattgaag
     4801 aactttcctt tgacgaagag gctcgacgtc tcgcaaaaag aacccaataa aacccttgcg
     4861 tccgctgggg agaccaccgt accgggctct tccggcatcg ggcgacactc ccaatagcga
     4921 atctttcttt gaaagatttt ctcgagggta tcgagacggt cgggacactc cagtgactca
     4981 gtcatatcga ttccacaggc attcatcaat tcccggcggc ctgagatcct ttccgacaag
     5041 aaattattaa agtttacgaa cgccagaaaa ttggatatcc gagcactcgt cacggcgcga
     5101 aacaaggcgg gagcgctttc cctgagcgag gaatacagaa agcgtaccgc ccggtccccg
     5161 tagagttttt ccgtaagaac cttaccggat tcccgatcca ggtattggtg atgtcctatc
     5221 ttcatcgtcg caaatacttt ttgataacga ctccgcctta cgtctttccg cgtacgtgtg
     5281 gattagagaa aggtgtatca gtttgacgag atctcccagg gaaacccgat cctctacgac
     5341 atcttcctcg atcggtctga gaaagtcctg gaggcgccgg ttgccgagcg ttgccctgag
     5401 ggcaaggatt gatgcagaat cggcgtcgtc cgaacacctt tcgatctttc tgaggccttt
     5461 ttccagacac tggagggcct ccgtcagatg tcgtttcctc agccgctcac ggtaatacct
     5521 ttcggccgcg ctgttaaatt cttttgcact cagcttcatg ggtgcgttcg tgttcgcttc
     5581 ttcgacaatc ccgcgagtca atctaccagc cgttgaaaac tcgtcgggat ggtccaggcg
     5641 gctcacgaga tccttaatag tttccttcat gcacaggata tcgatgaggt cggcggcgtc
     5701 ttcttctatg atacggacca atgccctctg gtattcttca ttgtgcaccc gtaagtatcc
     5761 gggataccgc ctgctcctac gcatgcggta tacccgcagg agaatttttt gcataaataa
     5821 gtttctcgtg tcgctcctaa cgaaaaaagt cggtatcccg gcagccgttc cgaagattac
     5881 ctgtcggcgt tcgctttcca caaaaggatc gtcgggaatc tgggcgtgtg tgcacagtcc
     5941 catggcaatg tatttatagg caagcgcggt aatcaggatc tgcagttgag cagcccttcc
     6001 catatcctct tcgtaattct cgaagagact gtaatggcgt ccctcaaaac ccgaaaatcc
     6061 cataacctcg tattcacgaa gcttgtatag tatataaagg gacatgtgcg catcgaagat
     6121 gccgagatcg gaaaggtctt ttttcagtcg gtcggagttt ccaatctttc cgtccagcgc
     6181 cgggctcttt tccgtgctca tgagcatgac cggataatcg ataaggcgta aatcgggaac
     6241 gtaatcaccc cgcaggccga aaagtccgct tattatccta tcaagccggg gaataccgaa
     6301 gggagtcaag ggtcgtccca tgaccccgac atgcgccttc ttcttccatc gccgccagaa
     6361 catccgcaaa tgggtgtaat cgagttcatg gggaagaaat cccagggcct tttcagggtg
     6421 aaaatcttcg aaatcgaaac gatacggcgc tgcactgtaa gtcccgacaa aaagcggcag
     6481 gaagtgttcc gttatcttaa tcaccaggtc acccacatat ttttcatgaa tgcgagtgaa
     6541 tcccgatgac ggatcggaca gcagctttga tagtcttcgg cttcccaggc tgatatgagt
     6601 cccattattg gcaaggctga tattcgaaag atttggtagg actaccagat tccgggtgag
     6661 tatgcccgct tccttgagct tggccacggc attgagctga ctcctgctca acaccccgtg
     6721 gcacagatgc atgtattcca gctttagttc tccccggtcc caacccgaca gacagggatt
     6781 catgaacagt tctctgtaaa aggcgtcaga aacgcaatcg ttaagccatt tctgccggac
     6841 aggaggatgg ggcgaagaga atatgacggc cttctgtccg ttcccggcca ggcaaaactt
     6901 ccggttggca tacatgatca gaagttgcgt aagaaggaat cgtatcaaag tttctccggc
     6961 aatccccttt cccgcgtcgc tgcccttgcc ggaaaagacc agccaaaagg aatgcgtctc
     7021 cggagatgtg ttgtcgctga gaaaatgttc catcaattcg cagcccgccc tgtgaaccgg
     7081 tcggggcaca tcagcgcccg cactaaccac atcggccaga gccagcttga gcaggtaact
     7141 gagcgggacc cggaggtatt cctcaccctc tgcggaaaca acgaacttat gggcatcgct
     7201 tctcaatgtg ttgttccggc tttgtttatc ggatagcagg tcattccgaa acacatcttt
     7261 agcaaaggag ctcaacacgg ccaggggaaa gcggacccaa ctgttttccc aaacgttgtc
     7321 ccgattagcc gccagccacc tttcgaggtc ggtaatgacc tttcggggtg tgtctccagc
     7381 cgctgtccgc ctcataatgt tcgcgtaata attggactgt tcaatcatga tgggcaggtc
     7441 aacattgtct cgtctgccga tgacggcggc ctggagttcc gtctctgtgc cggccgttgc
     7501 atcactgccg gagaagggaa gcgtctcaac cagcgcccca acgcttcggg tgtcgattcc
     7561 catcagttcg atgatctttt tcagaaattc gggatcgttg gacggcgatg cagcccttcg
     7621 ctcaagacac tcggtaacgg tgtgaagccg actcatcgtt catcctccct ttcatattag
     7681 aaagtatctt cattccgacc ttctcggtca atttacaggg acaaactctg cgtggttttg
     7741 acgatttcat aagaatgcct tttctggttt tcgcctgatc ccctgccgta atagtgccat
     7801 ttcggaaaat tgaaatccga ggtcacggat gtcggcaaaa ggagcacggc ctgcagaaag
     7861 gactcaaggt ccgggacatt gtaaatgttg gcttcaaacc cccatttgtc cagcttgtcg
     7921 agaacgcgat ggatattcga tgatttccag ctcatggaaa aacgatttac gccccaactt
     7981 ctaaacttgt cgagaagggc cagggcttct ccggggacac tcaagatcag gggaacgagg
     8041 caatcgatag ggacctgaat aatggattgt gggaaggcct tggtcagttt gctaaaccct
     8101 tcctgtttca attcgtcgac
//
LOCUS       DAOTMX010000033         7961 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_8087_length_7961_cov_3.793322, whole genome shotgun sequence.
ACCESSION   DAOTMX010000033 DAOTMX010000000
VERSION     DAOTMX010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 7961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7961
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8087_length_7961_cov_3.793322"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1045
                     /locus_tag="P5573_01735"
     CDS             <1..1045
                     /locus_tag="P5573_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRT26816.1"
                     /translation="IFFAFTFAIYTGATALPETLHKAPIAFVDEDRSPLSARIIDAFY
                     PPYFMPPKMITRAEMDARMDAGLDTFAVNIPPDFQRDVLAGRSPAIQLNVDATRMSQA
                     FTGSGYIQTIIDGEVSSFLQGYRAYQAPPVDLALRARFNPELNRIWFGAVMKIIDNVT
                     LLSIILTGAALLREREHGTVEHLLVMPVTPFEIMTGKVWAMALVVLAATAFSLLFIVQ
                     GLLRVPIEGSVALFLVGTTLHLFATTSLGIFLGTFARSMPQFGLLMMLVLLPLQILSG
                     STTPRESMPEFIQVVMLGAPNTHFVMFAQAILYRGAGISVVWPQFLFLALIGSILFGL
                     SLARFRKTLATMA"
     gene            complement(1123..1698)
                     /locus_tag="P5573_01740"
     CDS             complement(1123..1698)
                     /locus_tag="P5573_01740"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HRT26817.1"
                     /translation="MKRKIIVCVLLLVAVISAAYAAEGKKALMVIASQDFRDEELLKP
                     KEILENKGVKVTIASSSLDTATGMLGAKVKPDVLIKDVNVNDFDAVIFVGGTGAAEYF
                     NNPVALKIAQDAYNRGKLVGAICIAPRVLSEAGVLKGKKATVYSTEAKALKAKGAIYT
                     GKDVEVDGKVVTASGPHAAEQFGKTIVKLLK"
     gene            complement(1777..3138)
                     /gene="mnmE"
                     /locus_tag="P5573_01745"
     CDS             complement(1777..3138)
                     /gene="mnmE"
                     /locus_tag="P5573_01745"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF003661.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HRT26818.1"
                     /translation="MAAVATPPGAGGIGVIRVSGPKAEEVGRLLFRPGKRPCPFATHH
                     FYHGDVLARESGAVLDEALVVLMRAPHSYTGEDTLEIHCHGSPAVLRAVLEEVLKAGA
                     RPAEPGEFTRRAFLNGRMDLSQAEAVADLVAARAAKAREVAIAQLKGGLAEKVKEMRA
                     VLEEQLAMLEAAVDFPEENIETEPPSEAAGKIGAVAAEIARLLATYDEGRLYREGIHV
                     VIAGRPNVGKSSLLNRLLGEERAIVTPIPGTTRDFIEEEIQVGGVAVRLADTAGIREA
                     GDVIEEEGIGRVWERVARADAVVFLLDGSEGLSSDDIRILEGLKGKKVIVAVNKSDLP
                     QRLGRADVEGVFPGPRPLMISAKFGSGIEDLKKRILETVLDGEEMGRGEEVLCNVRHK
                     DAFERTAEFLAQAGESLASGLSPEFAAQDIRDALRCLGEITGETTSEDILDRIFASFC
                     VGK"
     gene            complement(3177..3884)
                     /gene="jag"
                     /locus_tag="P5573_01750"
     CDS             complement(3177..3884)
                     /gene="jag"
                     /locus_tag="P5573_01750"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HRT26819.1"
                     /translation="MNALEVEGKNIDEAIQNACREFNVPREKLNIEILSEGSSGLLGL
                     VGTKKARIRASLLTFEMGTEAAKETAEATPEKPSEKTTETGDTAEKAREILTGILSRM
                     GMDFPVTVEETDEAVTLNIKGDGSGLLIGKGGQTLDAIQYILNKAVGKPGRDRRMIVL
                     DTEDYRDRRDEYLVALAQKLGQKVKKSKKPITVNHLSARDRRVIHIALKDDEALTTKS
                     RGEGMYRKIIILPKKKE"
     gene            complement(3898..5514)
                     /gene="yidC"
                     /locus_tag="P5573_01755"
     CDS             complement(3898..5514)
                     /gene="yidC"
                     /locus_tag="P5573_01755"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HRT26820.1"
                     /translation="MDKKTVLAIVLSFAVVLVFQYFFVKPAPQQPTTSSSKEGQVAPQ
                     QTQAKSQPVAAITPAPAKAPRHAVVSMPAREVRVETPLYVAIFTTKGAGLKSFKLKSY
                     RKTLDENSDLVDLVEVKEGMAPPLAVSFVDSSVNIAPDSVYRADADSVVLAQAGESGR
                     LNFSLATPEGVQVEKTFIFYADRYHVDLEVRVFNPTAANLNEKAQLSWNQYVDPKAVE
                     DSYGHAGPVYSIKDDVETVTVSKMEARKVFPPPVSWGGFESKYFIAALIPKQPSLTGL
                     ITAKDASNQVTVALEGPANIVPTGQAAVFGYSLYLGPKEHKALAAEGVGLENAIDFGS
                     WIKWLALPLLIALNFLYKFVNNYGVAILILTAVIKVIFWPLGNKSYKSMKDMQKLQPL
                     INELRTKYKDDKQRLNQEVMQLYKTYKINPLGGCLPILIQIPVFFGLYKALLYSIELR
                     HQPFVWWIQDLSAKDPYYITPIIMGATMFWQQKMTPTAGDPMQQKVMLIMPIIFTFLF
                     LNFPSGLVIYWLFNNILSIGQQYYINKKLI"
     gene            complement(5546..5764)
                     /gene="yidD"
                     /locus_tag="P5573_01760"
     CDS             complement(5546..5764)
                     /gene="yidD"
                     /locus_tag="P5573_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002474682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HRT26821.1"
                     /translation="MKMLAKITIFLIKGYQLVLSPLFSPCCRFYPSCSEYTIQAVARY
                     GFLKGLYRGLTRLLRCHPLSQGGYDPVR"
     gene            complement(5770..6117)
                     /gene="rnpA"
                     /locus_tag="P5573_01765"
     CDS             complement(5770..6117)
                     /gene="rnpA"
                     /locus_tag="P5573_01765"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HRT26822.1"
                     /translation="MKRHSFRKSERILKRKDFSAAYDRGKRLSSANFIVFLHPNESGI
                     PRLGVTVSKKVGEAVKRNRIKRLLREFFRLNKDRLPPGQDVVIVARKDTSTKKYDEVR
                     RELETLLMAGAAD"
     gene            complement(6192..6329)
                     /gene="rpmH"
                     /locus_tag="P5573_01770"
     CDS             complement(6192..6329)
                     /gene="rpmH"
                     /locus_tag="P5573_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HRT26823.1"
                     /translation="MKKTLRNTSNIKRKRTHGFLVRMATRGGRLVLSRRRAKGRKRLT
                     V"
     gene            6665..7660
                     /locus_tag="P5573_01775"
     CDS             6665..7660
                     /locus_tag="P5573_01775"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HRT26824.1"
                     /translation="MFLKEKCDLCGDCLVNCKYLTYDKESAGAEIRRLINGEKAEILS
                     SCITCCACNEYCPTGANPFDLINELQERYFAFFVPKTSWDFYNSVIASPGRVTEGDPD
                     KPALSLCVMPPVLPKDLISGRMFEGLTLAQGGDYFCWFGYVHFGTETPVRKNAQKFVD
                     AIASLNRREVVFLHDDCYAMIKSKAPEYGIPVPFKPIHILEYIADYLKDHESEIKKLS
                     MRVAYQRPCASRYTPEKEKLVQEIFDLVGVKWVDQKYKGVDSLCCGVLTLNADPERGR
                     AIMNRNLSDMEEHGAEGVVFLCPVCQYGLSEQAAQRGMRPIHIIDLAKLALGEIL"
BASE COUNT         1586 a         2363 c         2088 g         1924 t
ORIGIN      
        1 gattttcttc gccttcacct tcgcgatcta taccggcgcg acggcccttc ccgagacact
       61 ccacaaggcc cccatcgcgt tcgtggacga agaccgttcg ccgctctcgg cacgcatcat
      121 cgatgccttc tatccgccct acttcatgcc ccccaagatg atcacacggg cggaaatgga
      181 tgcccggatg gacgccggcc tcgacacctt tgcggtcaac atcccccccg acttccagcg
      241 cgacgtcctt gccggccgct caccggcgat ccagctaaac gtcgatgcca cccggatgag
      301 ccaggccttc accgggagcg gttacatcca gacgataatc gacggggagg tctccagctt
      361 cctccagggg tatcgcgcct accaggcccc tcctgtggac ctggccctgc gggcgcgatt
      421 caacccagag ctgaatagga tatggtttgg ggcggtcatg aagatcatcg ataacgtgac
      481 cctactctcg atcatcctca ccggcgccgc gttgctccgg gagcgggagc acgggaccgt
      541 ggagcatctg ctggtcatgc cggtgacccc cttcgagata atgaccggca aggtatgggc
      601 tatggcgctc gtcgtgctcg ctgccacggc cttctcgctg ctcttcatcg tccaggggct
      661 gctcagggtt ccgatcgaag gctcggttgc cctgttcctg gtcgggacga cgctccacct
      721 atttgcgacg acgtccctgg gcatcttcct gggcaccttc gcccgctcca tgccccagtt
      781 cggcctcttg atgatgctcg tgctcctgcc cctgcagatc ctctcgggaa gcaccacacc
      841 ccgggagagt atgccggagt tcatccaggt cgtgatgctg ggggcgccga acacccattt
      901 cgtcatgttc gcccaggcga tcctgtaccg cggggcagga atttcggtcg tctggccgca
      961 gttcctcttc ctggccctca tcggctccat cctctttggc ctgtcgctcg cccgcttccg
     1021 caagaccctg gcgacgatgg cctgagggct atacgcggcg acggccttgg ggacggcgtc
     1081 gaggccggga caaggcacgg cccgccgtta aaatctctcg tattatttca gcagcttaac
     1141 gatagttttg ccgaactgct ccgccgcatg ggggcccgat gccgtcacga ccttcccgtc
     1201 gacctcaacg tccttccccg tgtagatagc cccttttgcc ttcaatgcct tcgcctcggt
     1261 gctatagacc gtcgctttct ttcccttgag gacccccgct tcggacagaa cccgcggcgc
     1321 gatgcatatc gcccccacga gcttcccccg gttatacgca tcctgggcga tcttcaacgc
     1381 gacgggattg ttgaagtatt ctgccgcccc ggtaccgccg acgaagatga ccgcgtcgaa
     1441 atcattcacg tttacgtcct ttatgaggac atccggcttg accttcgcgc cgagcatccc
     1501 ggtagcggtg tcgagggaag aagaggcgat cgtcaccttg acccctttgt tctccagtat
     1561 ttctttcggc ttcaggagct cttcgtccct gaagtcctga gaggcaatga ccatcaatgc
     1621 ctttttccct tccgccgcat aggccgcgga gatcacggct acgagcagta aaacgcagac
     1681 gatgattttt ctcttcattt ccgatcaacc tccttatgct ttattttcgc gcttaaatcg
     1741 tgacaatccc cgtcaaaggc tgcgatgcca aaaaaatcac ttgccgacgc agaaagacgc
     1801 gaagatccgg tctaagatgt cttcgcttgt tgtctctccg gttatttcac ctaggcaccg
     1861 caaggcatcc ctgatgtcct gggcggcgaa ctccggcgag aggcccgaag ccaggctctc
     1921 cccggcttgt gcgaggaatt cggctgtcct ttcgaatgcg tccttgtggc gcacgttaca
     1981 gaggacctct tccccgcgtc ccatctcttc tccatcgagg acggtctcta atatcctttt
     2041 tttcaggtct tctattccgc tgccgaactt cgccgagatc atgagaggtc ttggccccgg
     2101 aaagacccct tcgacgtccg cccggccgag gcgctgcggc aggtcgctct tgttgaccgc
     2161 tacaatgact tttttcccct tcaacccctc cagtatccgg atgtcgtcgg aggaaagacc
     2221 ctcgctcccg tccaggagga agacgacggc gtccgcccgc gcgacccttt cccagacccg
     2281 gcctatgcct tcctcttcga tcacgtctcc ggcctccctg atgcccgccg tgtctgccag
     2341 gcgaacggcc acgccgccga cctgtatctc ctcttcgatg aaatccctcg tggtgccggg
     2401 gatgggtgtc acgatagccc tttcctcccc gagaagccga ttcaggaggc tcgacttccc
     2461 cacattgggc cttccggcga tcacgacgtg aatgccctca cggtagagac gcccttcgtc
     2521 ataagtagcg agcaatcttg cgatttccgc tgctacggct cctatctttc ccgccgcctc
     2581 ggacggcggc tccgtctcta tgttctcctc agggaaatcc acggccgctt ccagcatggc
     2641 cagttgctcc tccagtaccg cccgcatctc tttgaccttt tcggcaaggc cgcccttgag
     2701 ctgggcgatg gcgacttccc gggccttcgc cgccctcgcg gcgacgaggt cggcaacggc
     2761 ttccgcctgg gagaggtcca tccggccgtt gagaaaagcc cggcgggtga actctcccgg
     2821 ttccgcgggc ctggccccgg ccttcaacac ctcctcgagc accgcccgca gaacggcggg
     2881 gctgccatga caatgtattt ccagggtatc ttcgccggtg taggaatggg gcgcccgcat
     2941 caggacgacc agggcctcgt cgaggacggc cccgctctct cgggccagga catcgccgtg
     3001 atagaaatgg tgggtagcaa agggacaggg ccgttttccg ggccggaaga gcagacgccc
     3061 gacttcttcc gctttcgggc cgctcacccg gatgacgccg ataccccccg cccccggcgg
     3121 cgtggcgacg gccgcgatcg tgtcttcttt cccggtcgaa accgcgcgga tgaggcttac
     3181 tccttctttt tcggcaggat tatgatcttc ctgtacatcc cctcgcccct gctctttgtg
     3241 gtaagggctt cgtcgtcctt cagggcaata tggatgaccc tgcggtcgcg ggcgctcagg
     3301 tggttgaccg ttatcggttt cttcgacttt ttgaccttct gccccagctt ctgcgccagc
     3361 gctaccaggt attcgtccct tcggtcccgg tagtcttccg tgtcgaggac tatcatcctc
     3421 ctgtccctgc ccggttttcc gaccgcttta ttgaggatgt actgtatggc gtcgagggtc
     3481 tggccaccct tgccgatgag cagcccggag ccgtctccct tgatgttcaa cgtgacggcc
     3541 tcgtccgttt cttcgacggt cacagggaaa tccatcccca tccgcgaaag aatccctgtc
     3601 aggatctccc gcgccttttc cgccgtatcc cccgtttccg ttgtcttttc agagggtttc
     3661 tccggcgtag cctccgccgt ttcttttgcc gcctccgtcc ccatctcgaa ggtcaggagg
     3721 ctcgcccgga tgcgggcctt tttcgttccc acgaggccca gcagcccgga agacccctcc
     3781 gacaggattt cgatgttcag cttctcgcgg ggcacgttga attccctgca ggcattctgt
     3841 atcgcctcgt caatgttctt tccttcgacc tcaagggcat tcatctgaaa cacctcgtta
     3901 gatcaatttc ttgttgatgt aatactgctg gccgatactc aggatgttgt tgaacaacca
     3961 gtagatgacc aacccggagg gaaaattcag gaacaggaag gtgaagatga tcggcatgat
     4021 caacatgacc ttctgttgca tcgggtcgcc tgcggtgggg gtcatcttct gctgccaaaa
     4081 catggtcgcc cccatgatga tcggtgtgat gtaatagggg tccttcgccg aaaggtcttg
     4141 aatccaccag acgaagggtt gatgacggag ctcgatggaa tagagtagcg ccttgtagag
     4201 cccgaaaaat actgggattt ggatgagtat cggcagacac ccgcccagag ggttgatctt
     4261 gtatgtcttg tagagctgca tgacctcctg gttgaggcgc tgcttgtcgt ccttgtattt
     4321 cgtcctcagc tcattgatca ggggctgcag tttctgcatg tccttcatcg atttgtagct
     4381 cttgttgccc aggggccaga aaatgacctt gatcacggcc gtcaggatga ggatggccac
     4441 tccgtaatta ttgacgaact tgtaaagaaa gttaagggca atcaggaggg gcaatgccaa
     4501 ccatttgatc caggagccga agtcgatggc gttttcgagg cctacgcctt ccgctgccag
     4561 ggccttgtgt tctttagggc cgaggtagag agaatagccg aagacggcag cctgacccgt
     4621 cgggacgatg ttggccggac cttccagggc gacggtcacc tggttggaag cgtcctttgc
     4681 tgttatgagg cctgtcagcg acggttgctt cgggatcaac gccgcgatga aatacttgct
     4741 ctcgaagcct ccccaggaaa caggcggagg gaagaccttc cgggcctcca tcttactgac
     4801 ggtcacggtc tccacgtcgt ccttgatgct gtaaaccgga cccgcatggc cgtaactatc
     4861 ctcaacagcc ttggggtcga cgtattggtt ccaggacaat tgcgcctttt cgttcaggtt
     4921 ggcggccgtg ggattgaaga cccggacctc aaggtcgaca tgataccggt ctgcgtagaa
     4981 gatgaatgtc ttttcgacct gcaccccctc cggcgtggca agggagaagt tcagcctgcc
     5041 actttcgccc gcctgcgcga gcacgacgga gtcggcatcc gccctgtaca cgctgtcggg
     5101 ggcgatgttt acgctcgaat cgacgaagga aacagcaagc ggcggggcca tcccttcttt
     5161 gacctccaca aggtctacta ggtccgagtt ctcgtccagg gtcttgcggt aggatttcag
     5221 cttgaaggat ttcaggccgg ccccttttgt cgtgaaaatt gccacataca gaggcgtttc
     5281 aaccctgact tcccgggcag gcattgagac gacggcatgg cgaggggcct tcgccggggc
     5341 gggggttatc gccgcgaccg gctgagactt tgcctgcgtc tgctgagggg cgacctgccc
     5401 ttccttcgag gaggacgtag tcggttgttg cggcgccggc ttgacaaaaa agtactgaaa
     5461 aaccaggaca acggcgaagg atagaacgat ggcaaggact gtctttttat ccattaagct
     5521 gcctccagat tccccgctgc atggactacc ttacggggtc atacccgccc tggctgagag
     5581 gatgacaccg gagcaacctc gtcaggccgc ggtagagacc cttcagaaaa ccgtatcgcg
     5641 cgacggcctg aatcgtgtac tccgaacagg atggataaaa tcggcagcac ggtgaaaaaa
     5701 ggggagataa aacgagttga tagcctttta tcagaaaaat ggtgatcttc gcgagcatct
     5761 tcatctgtat caatcggccg ctcctgccat gaggagggtc tccagctccc gccttacttc
     5821 gtcgtacttt ttcgtcgagg tgtctttcct tgcaacgatg acgacatcct gtccgggcgg
     5881 gagcctgtct ttgttcaacc taaaaaattc cctcaaaagg cgcttgatgc ggtttctctt
     5941 caccgcttcg ccgacctttt tgctgaccgt taccccgagc ctcgggatgc cggactcatt
     6001 ggggtgcaaa aaaacgatga aatttgcaga ggaaagccgc tttcctctgt cgtaggcggc
     6061 ggaaaagtct tttctcttca gtatcctttc gctcttcctg aaagagtgtc ttttcatctt
     6121 ccccgcagac cgggctttct taaaatcacc atgccctacc tgccggcttc cgcccgtccc
     6181 cggaacgcgc cctacacggt caatcgcttg cgcccctttg cccgccttct actcaggacc
     6241 aatcttcccc ccctggtcgc catcctcacg agaaaaccat gggtcctctt tctctttatg
     6301 ttactggtgt tccttaaagt cttcttcata acgtcgatcc tgtcgatcct attgaaagtt
     6361 gaagcagtct tctaaaccat agtcgccgga gtttgtcaag gaattattga aaatttcgtt
     6421 atcgcacccg tcacctccgg cattccggca accgaactgc cgcctttctt tttatcagga
     6481 cacatcctcg tcttctatgt ctccgcacta tcctgattcg gcgtccaaga aaaactctat
     6541 accaatataa ttacttcttg acataagttt aatatataga ttaaaaaagc atacattgag
     6601 tataaaaaca cttttatgat ttttttaaga accttgagcg ggtgaagaaa aggagggtga
     6661 gattatgttt ttgaaagaga aatgcgacct atgcggtgat tgtcttgtca attgtaaata
     6721 tctaacttat gacaaggaat ccgccggtgc ggagataaga cgcctgatta atggggagaa
     6781 agcagagatt ctttcttcct gtattacctg ctgcgcctgc aatgaatatt gtcctacagg
     6841 ggcaaatcct ttcgatctga taaacgaact ccaggaaagg tacttcgctt tttttgttcc
     6901 caaaacctcc tgggattttt acaacagtgt tatcgcctca ccgggccgag taacagaggg
     6961 ggatcccgac aaaccggcat tgtcactttg cgttatgccc ccggttcttc caaaagatct
     7021 gataagtggt cgtatgtttg agggtttaac ccttgcacaa ggcggggatt atttttgctg
     7081 gtttggttac gtccatttcg gaacggaaac cccggttaga aaaaatgctc aaaaattcgt
     7141 tgatgcgatt gcgagcctta atcgtcgcga agttgtcttc cttcatgatg actgctatgc
     7201 catgatcaag agcaaagcgc cggaatacgg aattcccgta cccttcaagc caatccatat
     7261 tctcgaatat atcgccgact acctaaaaga ccatgaatcg gaaatcaaaa agcttagtat
     7321 gcgggtagct taccagcggc cctgcgcatc aagatatact cctgaaaagg aaaagttggt
     7381 tcaagagata ttcgatttag tcggtgtaaa atgggttgac caaaagtata aaggggtcga
     7441 ctcgctatgc tgcggtgtcc tgactttaaa tgcagacccg gaaagaggac gggctatcat
     7501 gaacaggaat ttgtcagaca tggaggagca tggagcggag ggggtggtct tcctgtgccc
     7561 tgtttgccag tacggactct ccgaacaggc tgcgcaacga gggatgaggc cgatccacat
     7621 catcgacctc gccaaattgg cattagggga aattctttag ggctcattgc atcgaagctt
     7681 cacccgtttt cgtctgacag aaaaaattgt gggtcctctt cggtaccggc tacgaacata
     7741 gacgtagccg accaaccaaa accatggtat aaaaactcgt ggacatactc cccccttcgt
     7801 atccggaaat ttttatgctt ttcctttttg gggtgcatgt ccatcttttt caaccgccaa
     7861 gcgaacgcac taaccggatg aaccgcctca ttttggacga ttatggttga tggactcgta
     7921 aaaagtcgag aattacgcgt ttcgtcattc cggcgaacgc c
//
LOCUS       DAOTMX010000034         7941 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_8108_length_7941_cov_4.242962, whole genome shotgun sequence.
ACCESSION   DAOTMX010000034 DAOTMX010000000
VERSION     DAOTMX010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 7941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7941
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8108_length_7941_cov_4.242962"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..1276)
                     /locus_tag="P5573_01780"
     CDS             complement(89..1276)
                     /locus_tag="P5573_01780"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HRT26825.1"
                     /translation="MPGALEGLKVLDFTTLLPGPYATMCLADMGADVLRVVSGSRPDI
                     VDFFPPLIPGMKFSAASAYLGRNKRVMALNLKDQRGIKIVHQLLGEYDVVVEQFRPGV
                     MAKLKLDYESLRAVNPAVIYCSISGYGQTGPMTDRAGHDINYIARSGIASYSGKKETG
                     PCLMGMQIADVASGSYNAVIGILAAAFYRKATGKGQYIDISMTDGMIAFNAMFGAGFL
                     VDGNEPEFESILLNGGSLYDYYRTKDGRFISFGGLEPQFFTNFFNTINRPDLIPGGVM
                     PENVAQVKQEVRDIILTKTRDEWVEIFRKTDACVEPVLTMTEAFSDPLVKEREDVVEL
                     PLPGGGKVKQLANPIKFSETKPEYRSVGVSVATGGHTKEVLRRLGYSEGEIEEFERTG
                     LFS"
     gene            1500..2180
                     /locus_tag="P5573_01785"
     CDS             1500..2180
                     /locus_tag="P5573_01785"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HRT26826.1"
                     /translation="MVLRIGHRGAMGYAPENTLLSFRKTLELRCDMIEFDVRLCRSGE
                     LIVFHDDTLERTTNGAGQVSEQTLEFLRTLDAGCGEKIPLFQEALEVIAGKARVDVEL
                     KGPGTALQTASLIDRFLTQGGWSRGHFLVSSFDCQELSAFRRLDPYTPVGILCENIPV
                     GFFGMARSLGAYSLNISYNGVTPELVDHIHREGMKVLVWTVDSPDGIARAKGMGVDGI
                     ISNFPDRV"
     gene            2331..2618
                     /locus_tag="P5573_01790"
     CDS             2331..2618
                     /locus_tag="P5573_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT26827.1"
                     /translation="MVRTGEEVHPCVGCGFCCIRQTCTFGCQRHPESLGKVCPELEWN
                     GNRYVCRLLKTPGPMVFFYKEELQVGGGCRSYLNPWRKDIRPRTKEDLTDL"
     gene            complement(2605..4056)
                     /locus_tag="P5573_01795"
     CDS             complement(2605..4056)
                     /locus_tag="P5573_01795"
                     /inference="COORDINATES: protein motif:HMM:NF023222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HRT26828.1"
                     /translation="MKTAQEYKDSIRAMKQVVYAFGEKVEQVADHPTFAPALNAIALT
                     YELAGEKENEGLMTVKSPFTGKLVNRFLHICLSPDDLIKRCELGKFLTPFHGACVGAR
                     CAGTAALNALYATTYDMDEKLGTDYHQRFLRFLTHAQDEDLTCSGMVTDAKGDRSLSP
                     AQQADRDVYLRVKEVREDGIVVRGAKAHQSGAPLAHFNIVAPTTSMRENEKEFAVAFA
                     VPANAPGVIHIAEAPAPNARRLFSRDVMDFGNHEYGVHGSTLTVFDDVFIPREMVFLC
                     GECEFTGPLASTFGAFQRLATSGCKSGHCDLTCGAAAVAAEYNGCEKMGHIRDKVVEM
                     SFQSALAFGTAVAAGYKARVTSSGVCVPNDLLVNAAKLQAVEAVWSASKLATEIVGGV
                     ICTAPSQKDFDNPEIAGYVDKYFRGKADVSTADRVRITRLVEYLVGQGSIIPTESTHG
                     GGPAAVQRLMIRQTNNLNYFKTRAKRMAGIKDR"
     gene            4306..4869
                     /locus_tag="P5573_01800"
     CDS             4306..4869
                     /locus_tag="P5573_01800"
                     /inference="COORDINATES: protein motif:HMM:NF027619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRT26829.1"
                     /translation="MDKKDEILQAALDLFVDQGFHGAPMADIAKRANVATGTIYRHFP
                     SKNDLINELFHNLEREIKTVIKVGNPAEGSIKEKFMFLMRETIRYFIDHPRHFLYMEQ
                     YFNSPYSVSIHRDIVLGKGREDVWWNVFERGMSEGVIKDLPRTMLFALAFGPMIFLLR
                     DHVRGFICLMDEEITRFAEICWDVIKK"
     gene            4898..6097
                     /locus_tag="P5573_01805"
     CDS             4898..6097
                     /locus_tag="P5573_01805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRT26830.1"
                     /translation="MSMMFCGKRIAFTKVWVLTILLMAVAGLFGCGQSKGGPVSQTPE
                     VAVVTLQSEPVALTTELAGRTVAYRVAEVRPQVNGIVLKRLFTEGSDVRIGQVLYQID
                     PAPFQAALDNARAALARSEANLPTVRLKEERFRNLLADKAVSQQEYDDAAAALNQTEA
                     DIKYWKAMVEQAKINLGYTAVTAPISGRIGRSTVTEGALVTAHQAVPLATIQQLDPIY
                     VDVPRSTTEVLQFRRELEEKKVIHRGESLKKVRLFLEDGSLYPLTGTLQFQEVTVDQS
                     TGSVMLRIVFPNPRWTLLPGMFVRASVETGVNPQAILCPQQAVMHNPKGEAYVYVVND
                     KNEVEMRTIVVERTIGDKWLVSSGLAAGERVVMEGLQRIKPGAVVKAVPFSSGNQPQP
                     PAGTKEG"
     gene            6104..>7941
                     /locus_tag="P5573_01810"
     CDS             6104..>7941
                     /locus_tag="P5573_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRT26831.1"
                     /translation="MLSRFFLDRPVFAWVIAIFIMVLGALAIYFLPISQYPNIAPPSI
                     YVRAYYPGASAETVENSVTQIIEQKMTGLDKMIYLSARSDSAGNSRLELTFAPGTDPD
                     IAWAKVQNKLQLAMASLPDVVQRGGVAVGKATRNYLLIVSLISEDGSMSSIDLRDYAQ
                     SNLEMSLSRVPGVGEIEAFGTQYAMRIWLDPDRLTDYRLTVEDVTQALRNYNVEVSAG
                     KFGGPPAVKGQRLNVNITIQSMLKTPEEFGRVPIRINPDGSIIRIRDVGRVELGTEKY
                     EVDNHFNGMPASGLAIRLEPGANALDTADRVKARLVELSRYFPHGLKVVYPYDTTPFI
                     RVAIKEVVKTLFEAIVLVFIIMWLFLGSVRATLIPTIAVPVVLLGTFAVIWIFGFSIN
                     MLTMFGMVLAIGLLVDDAIVVVENVERIMSEEGLSPREATAKSMDQITPALIGIGLVL
                     AAVFGPMAFFAGSTGILYRQFSVTVATAMLLSVLVALILSPVLCASFLEPVSKGHDAA
                     EKATPFLRPFLLWFDRWFYKIRDYYVRFTGSVLIRRVRYVVVFLVIVVALGYLFMRMP
                     TSYIPAEDQGTLLAQVILPAGSTLEQTQVVADEIQNHFMEKEKEAV"
BASE COUNT         1691 a         2288 c         2230 g         1732 t
ORIGIN      
        1 cgcccactcc ctgagttgac ccgtgtattc cgtcgcctta tccttctcga cgttggcgat
       61 gatcccgatc cttctgatct gcatcaactc aactaaagag tccggtcctt tcgaattcct
      121 cgatctcgcc ctccgaatac cccagacggc ggaggacttc tttggtgtgg ccgccggttg
      181 caacggaaac gccgacgctc cggtattccg gcttcgtctc cgaaaacttg atcgggtttg
      241 cgagttgctt cacttttccc ccgccgggca acgggagctc caccacatcc tcacgttcct
      301 tcaccagtgg gtcgctaaag gcctctgtca tggtcaggac cggttctacg caggcgtcgg
      361 tcttgcggaa gatctccacc cattcatcac gggtcttcgt caggatgata tccctgactt
      421 cttgtttgac ctgggccacg ttttccggca tgacaccgcc cggtatcagg tcggggcggt
      481 tgatggtgtt gaaaaagtta gtgaaaaact gaggttccaa ccccccgaag ctgatgaacc
      541 gaccgtcctt cgtccggtag taatcgtaga gcgaaccgcc gttcagaagg atgctctcaa
      601 actcgggttc attgccgtcg acgagaaaac cggcgccgaa catggcgttg aaggcgatca
      661 tgccgtccgt catcgatatg tcgatatact ggcctttccc cgttgccttt ctgtaaaagg
      721 ccgccgccag gataccgatc accgcgttat aagagccgga cgcgacgtcg gcgatctgca
      781 tccccatgag acagggtcct gtctcttttt tacccgaata agaggcaatg cctgaacggg
      841 ctatgtagtt gatgtcgtga cccgcccggt ccgtcatcgg gcctgtttga ccgtacccag
      901 agatggaaca gtatatcacc gcgggattca ccgcccttag gctctcgtaa tccagtttta
      961 gtttcgccat gacgccgggg cgaaactgct cgacgactac gtcatattcg ccaaggagtt
     1021 gatggacgat cttgatccct ctctgatcct tgaggttcag ggccatcacc cgtttgttac
     1081 ggccgaggta agcggaggcc gccgagaact tcatgcccgg aatcagcggc gggaaaaaat
     1141 ctacgatgtc cggccgcgag ccggagacga cccgcagtac gtcggccccc atatcggcca
     1201 ggcacatggt cgcatagggt ccgggtaaca acgtcgtgaa atccaaaacc ttcaatcctt
     1261 caagcgcacc aggcataatc cctcctttat aaggtaataa agtgatttaa agaaccgaag
     1321 cccctgccgt acttttgatt ttcgggtcct atatttgaac aatgttgttc gtctgtcaat
     1381 cgaaaacctt caagaaaaag agttgatgag gcccgctctc ttgtcttaga atctcgccgt
     1441 tcaccgaatg ttcttgcccg ccgcatttct ttgggacgtc ttacgacagg aggtatcggt
     1501 tggtcctgag aatcggacat cgcggggcga tgggttatgc cccggaaaac actctgcttt
     1561 cgttccggaa gaccctcgag ctccggtgcg atatgatcga attcgacgta cggttgtgca
     1621 gaagcggtga gctcatcgtc ttccatgacg acaccctgga gaggaccacg aacggcgccg
     1681 gtcaggtttc ggaacaaact cttgaattcc tcaggacgct ggacgccggc tgcggagaaa
     1741 agatacctct ttttcaggag gcgctggagg tcattgccgg caaggcgagg gtcgatgtcg
     1801 agctcaaagg accggggacg gccctacaga cagcaagcct catcgatcgc ttcctgacgc
     1861 agggcggatg gtccaggggg cattttctgg tctcctcttt cgactgtcag gaactcagcg
     1921 ccttcagaag gctcgaccct tacacgcccg tgggaatcct ctgcgaaaac attcccgtcg
     1981 gctttttcgg catggcccgg agcctggggg cttactccct caacatctct tacaacgggg
     2041 taacgccgga actggtcgat cacatccacc gggaaggcat gaaggtcctg gtctggaccg
     2101 tcgactcgcc cgacggaata gcccgggcaa agggaatggg cgtagacggc atcatcagca
     2161 atttccccga ccgggtttga aggaacggcg gcaggcggaa ggaaccgcag cggacacccg
     2221 tgcctttgca aaacttttcg caaaactttt cattgatagg ccggtcgcca actgttatgt
     2281 accggcgtca gttccccgtt aacgaacctt ggcagagaga cggagatgat atggtgagaa
     2341 ccggcgagga agttcacccc tgcgtgggat gcggattctg ctgcatccgc caaacctgta
     2401 ccttcggttg ccagcgccat ccggagtcgt tgggaaaagt ctgcccagaa ctggaatgga
     2461 acggcaacag gtacgtctgc cgcctgctga agacgcccgg accgatggtc ttcttctaca
     2521 aggaggagct ccaggtcggc gggggctgcc gctcttacct caacccctgg agaaaagata
     2581 tccgtccccg aacgaaagaa gacctcaccg atctttgatg cccgccatcc tcttcgcccg
     2641 tgttttgaaa tagttcaggt tgttcgtctg acggatcatc aacctctgaa cggccgccgg
     2701 ccctccgccg tgcgtggact cggtggggat gatcgacccc tggccgacaa ggtactccac
     2761 caggcgcgtg atgcgcaccc tgtccgccgt cgatacgtcc gcctttcctc tgaagtactt
     2821 gtccacgtac ccggctattt cagggttatc aaaatctttc tgactagggg cagtgcagat
     2881 cacgccgccg acaatctcag tggccagttt cgacgccgac cacaccgctt ccacggcctg
     2941 cagtttggcc gcattcacga gtaagtcgtt aggcacgcac acccctgacg acgtaacccg
     3001 cgccttataa ccggcggcaa ctgccgtccc gaaggccagg gccgactgga aggacatctc
     3061 tacgactttg tcgcggatat ggcccatctt ttcacaaccg ttgtactcgg cggcaacggc
     3121 tgccgcgccg caggttaggt cgcagtggcc cgatttgcat cccgaagttg ccaggcgctg
     3181 gaaggcgccg aaggtggacg ccaggggccc ggtgaattca cactcgccgc agaggaaaac
     3241 catctccctg gggatgaaga cgtcgtcgaa caccgtcaac gtagaaccat gcacgccgta
     3301 ttcatgattg ccgaaatcca tcacgtcccg gctgaataac ctgcgggcgt tcggggcagg
     3361 cgcttcggcg atatggatga ccccgggggc attggcgggg acggcgaagg ccacggcaaa
     3421 ctccttctcg ttttccctca tggacgtcgt cggcgccaca atgttgaaat gggcaagggg
     3481 agcccccgac tgatgcgcct tggcgccccg aacaacgatg ccgtcctcgc gcacctcctt
     3541 cacacgcaga tagacatccc tgtccgcctg ctgggcgggg ctgagagacc gatcgccttt
     3601 tgcatcggtc accatcccgc tgcaggtcag atcctcgtcc tgcgcatgag tcaggaatct
     3661 taaaaacctc tggtggtaat cggtccccaa tttctcatcc atgtcatagg tcgttgcgta
     3721 gagcgcgttc agcgccgcgg tgcccgcaca acgagcgccg acgcaggcgc cgtggaatgg
     3781 cgtgaggaat ttgcccaatt cgcatcgctt tatcaagtcg tcgggagaga gacagatgtg
     3841 caaaaatcgg tttacaagtt ttccggtaaa cggcgacttg acggtcatca aaccctcgtt
     3901 ttctttttcc ccggccagtt cgtaagtcaa ggcgatggcg ttcagtgccg gggcgaaggt
     3961 agggtgatct gccacctgct ccaccttttc cccgaaggca tacaccacct gcttcatggc
     4021 tctgatgctg tctttgtact cctgagctgt cttcatgatg ccatcctttc tgcctgaaaa
     4081 aagagttcac taccaccacg gctgcggctg attcctgctc cagccgagac ccggccggaa
     4141 atcatccccg ggcgattacc ttacagtggt ctatatacgc atccgggcct acgtttcaag
     4201 gcaagttatc gaagccaaca cacataattt gtccttgaca aggactggtg aataaaatat
     4261 aagcgcgcga atgaacgttc attcgcgttc ttttggtgaa gaggaatgga taaaaaggac
     4321 gagatactcc aagcggcgtt agacctcttc gtggatcagg gattccacgg ggccccgatg
     4381 gccgacatcg ccaaacgcgc gaacgttgct acaggaacca tctaccgcca ctttccgagc
     4441 aaaaacgacc tgataaacga gctttttcat aacctggaac gagaaatcaa gacagtcatc
     4501 aaagtgggga accccgctga ggggtcaatc aaggaaaagt ttatgttcct catgcgggaa
     4561 actatccgct atttcatcga ccatccccgg cactttctct acatggagca gtacttcaac
     4621 tcaccgtaca gtgtttccat ccatcgggac atcgtcctgg gaaaaggtcg ggaagacgtc
     4681 tggtggaatg tgtttgaacg agggatgtca gaaggcgtga taaaagacct tccccggaca
     4741 atgttgtttg ccctcgcttt tggaccgatg atctttctgc tgcgggacca tgtccgtggc
     4801 tttatctgtc ttatggacga ggagataacc cgctttgccg agatatgctg ggacgttatt
     4861 aaaaagtgaa tcgttacaga acgagggtag ggagacgatg tcaatgatgt tttgtggaaa
     4921 gaggattgct tttacgaagg tctgggtcct cacgatattg ctcatggcgg tggcgggtct
     4981 tttcggctgc gggcagtcga aaggcgggcc tgtatcgcaa acgccggaag ttgcggtcgt
     5041 aacccttcaa tcggagccgg ttgctcttac gacggaactc gccggacgaa cggtggctta
     5101 tcgggtggca gaggtacgtc ctcaggtaaa cggcatcgtg ttaaagaggc tctttaccga
     5161 gggaagcgac gtacgaattg gtcaggtcct ttaccagatc gaccccgccc cctttcaggc
     5221 ggccctcgac aacgcccgag cagcgttagc ccgttcggag gcgaacctcc cgacggtcag
     5281 attgaaggaa gagcgtttcc gtaacctctt ggccgacaag gcggtaagcc agcaagagta
     5341 cgacgacgcg gcggcggccc tcaatcaaac ggaggcggac atcaaatact ggaaggcgat
     5401 ggtggagcag gcgaaaataa acctcggata cacggcggtt acggccccta tttcgggtcg
     5461 tatcgggagg tccaccgtaa cggagggagc gctggtcacg gcccaccagg cggtaccgct
     5521 cgccaccatc cagcaactgg acccgattta cgtcgatgta ccccggtcca cgacggaggt
     5581 tctccagttt aggcgggagt tagaagagaa gaaggtgatc caccggggcg aaagcttaaa
     5641 gaaggtccgt ctctttcttg aagacggcag tctctatcct ttgacgggga cgctccagtt
     5701 tcaggaagtg acggtcgacc aatcgacggg ttccgtcatg ctgcgcatcg ttttccccaa
     5761 tccacgctgg acgctcctgc cggggatgtt cgtgcgggcc tcggtcgaga cgggcgtcaa
     5821 cccgcaggcg atcctctgcc ctcaacaggc cgtgatgcac aacccaaagg gggaggctta
     5881 tgtatacgta gtaaacgata aaaacgaagt cgagatgcgc accatcgtcg tcgagcggac
     5941 cataggagat aagtggctcg tgagctccgg gcttgccgcc ggtgaacggg tcgtcatgga
     6001 aggcctgcag cggataaagc cgggcgcggt cgtcaaggcc gtgccttttt cttccggcaa
     6061 tcaaccccag ccgccggccg gcactaagga ggggtaataa agaatgctct cgcgcttttt
     6121 cctcgaccga cccgtatttg cctgggttat tgctatcttc attatggtgt taggggccct
     6181 cgccatctac ttcctgccga tctcccagta cccgaacatc gcccccccct ccatctacgt
     6241 gcgggcttac tatcccgggg cctcggcaga gacggtggag aacagcgtca cccagatcat
     6301 cgagcagaag atgacgggac tggataagat gatctacctt tccgcgagga gcgactccgc
     6361 cggcaattcc cgcctcgagc tcaccttcgc cccggggaca gacccggaca tcgcctgggc
     6421 gaaggtccag aacaagcttc agcttgccat ggcgtctctc ccggacgtgg ttcagcgcgg
     6481 gggtgtagcg gtggggaagg ccacgaggaa ctacctcctc atcgtaagcc tgatctctga
     6541 ggacgggagc atgagttcca tcgacctgcg cgattacgcc cagtcgaacc tcgagatgag
     6601 tctctcccgg gttcccgggg tgggtgagat agaggcattc ggcacccagt atgccatgcg
     6661 catctggctc gaccccgacc ggctgacgga ctaccgcctc acggtggaag acgtcacgca
     6721 ggccttgaga aattacaatg tggaagtgtc agccggaaag ttcggaggtc cgcctgccgt
     6781 aaaggggcag cgactgaacg tgaacatcac catccagagc atgctgaaga ctcccgaaga
     6841 attcgggcgg gtgccgattc ggataaatcc cgacggttcc atcatacgaa tccgtgatgt
     6901 gggccgggtg gaactaggta cggaaaaata tgaagtcgac aatcatttca atggaatgcc
     6961 ggcatcaggg cttgctatta ggttggaacc gggggccaat gccctcgaca cggccgatcg
     7021 tgtaaaggca cgccttgtcg agctctcccg ctatttcccc cacggcctca aagtggttta
     7081 tccctacgac accacccctt tcatccgtgt ggctataaag gaggtggtaa agaccctatt
     7141 cgaggcgatt gtcctcgtct ttatcatcat gtggctattc ctcggtagcg ttcgggcgac
     7201 cctcatcccc acgatagccg tgcccgtcgt cctcctgggc accttcgccg tgatatggat
     7261 atttggcttt tccatcaaca tgctgacgat gttcgggatg gtgctcgcga tcgggcttct
     7321 cgtcgacgac gccatcgtag tcgtcgagaa tgtggagagg ataatgagcg aggagggcct
     7381 tagcccgcgg gaggcgacgg caaagtccat ggaccagatc acgcccgccc tgatcgggat
     7441 cggtctcgtg ctcgccgccg tcttcggccc catggcattc tttgccggtt caacggggat
     7501 cctttaccgc cagttctccg tcaccgtagc tacggctatg ctcttgtcgg tccttgtggc
     7561 cttgatcctc tcccccgtct tatgcgccag tttcctggaa cccgtgtcta aaggacacga
     7621 tgcggcggag aaggcaacac cttttttgcg tccctttctc ttgtggttcg accgatggtt
     7681 ctacaaaata agggactatt atgttcgttt caccggctcc gtattgattc gtagggtgcg
     7741 gtacgtagtt gttttcttgg tgatcgtcgt tgctctcggg tatctcttca tgagaatgcc
     7801 gacgagctac atccctgccg aggaccaggg aacgctcctc gcccaggtga tcctgccggc
     7861 cggctccacc ctggagcaga cacaggtggt ggccgatgaa atccagaacc acttcatgga
     7921 aaaggaaaaa gaggccgtcg a
//
LOCUS       DAOTMX010000035         7930 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_8120_length_7930_cov_4.239365, whole genome shotgun sequence.
ACCESSION   DAOTMX010000035 DAOTMX010000000
VERSION     DAOTMX010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 7930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7930
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8120_length_7930_cov_4.239365"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..1803
                     /locus_tag="P5573_01815"
     CDS             112..1803
                     /locus_tag="P5573_01815"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRT26832.1"
                     /translation="MRRVSVFSAVVVGMVLLLVVRQGITLYMSDPATHLIRDRIKGID
                     AATLVEGEPLHNPDLVAGFYSEEGFRPVWSSEKHLTSKAEAFLAVLRGADDEGLNPKD
                     YHLARVESLVRQWRNGERQVEKRIDLDLLLTDAFVAFGRHVRSGRVDPRMVYPDWYNF
                     PRRENGDGEILKVLKQDELGKALKVIQPADSGYERLREALFRYRNIERRGGWPSVPGG
                     PKLKAGDRDDRVLLLRKRLSLSGDLDDGAEGGSLFDEKLDRALRRFQTRHGLVADGVL
                     GASTVEELNIPVEKRVRQLKLNLERLRWLPRVLGDRYIFVNLADFSLEVVEKDETVMT
                     MRIIVGRDEDDQRTLVFTSNITYLEINPFWNVPESIAVNEIIPKVKDDPHYLSSRGIR
                     IIDGWSEPAKEVDPESIDWESLDPGQFRYRLRQDPGPRNPLGRVKFMFPNEFNIYLHD
                     TPDRQLFERERRAFSHGCIRIEKPLDLAAYLLKGYSDWKREDILEAVASRQRQVVTLA
                     EPVPVYIFYLTVWVDRNGLVHFCRDVYNGDRMLEQAMNYKPVRRAHLPAPAKFPG"
     gene            1887..3134
                     /locus_tag="P5573_01820"
     CDS             1887..3134
                     /locus_tag="P5573_01820"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRT26833.1"
                     /translation="MKNTRSYQSPSFNKYPSSYIYLKHSHLKFAAIGFAVLLLSNAAL
                     VYLLIRGEMKNRALMDLVAHTGQRLEAIDKSLGEKDLQKAFGELQEVKKQVAVVLKPQ
                     PQVEKPVVPVAAVEAPAQKKVLPPQASVSTPDLSQETPYPFVYAGEGENALICEKETK
                     ILYVFRKSGGKWTLVKAYPCLTGANQQDKRKNGDLATPLGTYLFLRFIPGKDLAENYG
                     FGAYVLNYPNFTDRREGRDGTGIWLHGHSAGKSLGDQIVNTKGCIVVSNDALKEMSQY
                     LKPRGTAIAVVNKLSYTKAASQEALSKELRGFIESWRRAWESRDVQKYMSHYAAEFVS
                     ADGMSYQAFKRHKERVNSGKKFIRVTTEDLMIMLPQEREGKVAIARFIQKYQSSNFKT
                     ESNKVLYLKKGQGGWQIIGESSF"
     gene            3104..4033
                     /locus_tag="P5573_01825"
     CDS             3104..4033
                     /locus_tag="P5573_01825"
                     /inference="COORDINATES: protein motif:HMM:NF028343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M99 family carboxypeptidase catalytic
                     domain-containing protein"
                     /protein_id="HRT26834.1"
                     /translation="MANHRRILILTLLVFAFIFLILFIADTLSREKNITVDKGSPGVQ
                     VKRFFAGTPQEVTVYFLRGREKGPTLLIFSGIHGDEKGGYLAADRYTNLNVRKGTLVV
                     VPRLNLPAIRKERREGFDGDMNRLFHLPDDSQTLQDSKVVNLAKSLIRDADYVLNVHQ
                     GDGFYSPRWVSHKRNPLKWGQCNVIDASSFDLPNGQKLELERFAKAVAARANAKIEDC
                     RYHFLVNNTNTAAEESRHKEQRKSLTYYALSRHNKVALGLEATKSCSLPEAVAFLTAV
                     INSVIEEVGISVVQLPSESPDHIRAEWQMAKGE"
     gene            4100..5029
                     /locus_tag="P5573_01830"
     CDS             4100..5029
                     /locus_tag="P5573_01830"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRT26835.1"
                     /translation="MTDEKYRLAEKLMQEKRYDEAVALYEELVRLNPGEDSHRLALAW
                     AYKDSGRLDEAVALFEELLEKELSRKLFTGFAFDELVRILREEKQYDRMIQICERAVA
                     AQPDDVSLLFTLGDAYLAGGFPVKAIDAFKKIIMMDPDASSFMSLGNAQVAAGDFEGA
                     EEAYEQAGSFDPSSSDVYYNRLGMAYLRAGYFDRAEIAFRKAIDCNATQPIYYCNVGD
                     ALAMEGRLDEAEAVYEEAAAINPGSRGVYLNRLGNTLHREKRYLQAIRVFKKAIEEEP
                     KNPFYHMHLARSYEAAGFPEEARRENEKAQSLK"
     gene            complement(5034..5549)
                     /locus_tag="P5573_01835"
     CDS             complement(5034..5549)
                     /locus_tag="P5573_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT26836.1"
                     /translation="MDEEKSLLEEVEAGGYDSADRPFDFIEENAETALLCCTVPEVRE
                     KVSAALNSLGFHISEASDARDALKKMRFHVYDVVVLDEYFDTGNPETNDVLAYLQGLS
                     MSTRRRMFVTLISSKYRTMDNMAAFYRSVNLVINPKNIDSAGVILKRGINDHVAFYRV
                     FHEMLKKTGKA"
     gene            complement(5561..6733)
                     /locus_tag="P5573_01840"
     CDS             complement(5561..6733)
                     /locus_tag="P5573_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HRT26837.1"
                     /translation="MRKPEIDYILSKMLDSSKNVSDLNITVGKPFQVESSGQLTPVQL
                     DPPVTRLTPFQTEIFALNLINGDHRLTETLLNTGSCDSSYELPGKARFRVNIFSQRGN
                     YSIVLRKLESKVPTCQELNLPPIFQQIAREKNGIVLVTGATGSGKSTSLAAILDEINE
                     EQAVHIITLEDPVEFSHTHKKATFNQREMGTDFDTFASGLRAALRQAPKVILVGEMRD
                     RETVEIGLNAAETGHLVFSTLHTIDAGSTINRIIGMFTTEEENQIRIRLADTVRWIVC
                     QRLLPKEGGGRVAAFEIMGTNLRVKDAILHGESEGKTFYEIIQAGKPFGMITFDDYIV
                     DLYKDGLISEETARAYASNKGIVGRGIDAVKSSRGQATTEIGKLTVDKDYGKKKSF"
     gene            complement(6747..7829)
                     /locus_tag="P5573_01845"
     CDS             complement(6747..7829)
                     /locus_tag="P5573_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HRT26838.1"
                     /translation="MAKIDAFFQLMNEQGASDLHLVAGQQPVLRIRGEMERVKYNVLD
                     NDELKAMLYEIAPEHKVKQFEETGDIDFAYEIPGLARYRANFFQQKYGVGAVFREIPT
                     KILTCAQLGLPAVVSKLASLPRGLVLVTGPTGSGKSTTLAAIIDEANRTRKDHIITIE
                     DPIEFVHQSQSCVVNHREVGLHTKSFSAALRGALREDPDIILVGEMRDIETISLAVEA
                     ASTGHLVFGTLHTMNAAKTVDRIIEVFPANEQGQIRSNLADGIRAVVSQVLFKRADKK
                     GRVAALEILIANSAVRNLIRESKTFQIPSMIQTGKKFGMQMLDDAIQDLLQRGMITPD
                     DAYLKCNDKQRFRPFLKTPPADFTEI"
BASE COUNT         1745 a         2112 c         2310 g         1763 t
ORIGIN      
        1 cataatcgct gacggctatt tagaatggcc aaaacatgct aatggaaaat ctgggataaa
       61 taaccattta aaatagttaa agtacgaaaa agggacggcg aaaagcaagt catgcgcagg
      121 gtttcggtct tctcagcggt tgttgtgggt atggtgctcc tcttggtcgt caggcagggg
      181 atcacattgt atatgtccga cccggcaact cacctgattc gcgacagaat caagggcatc
      241 gacgctgcga ccctcgtcga gggtgagcct ctccacaatc ctgacctggt tgccggattc
      301 tattcagaag agggttttcg gcctgtctgg tcttcggaaa agcacctgac gtcgaaggcg
      361 gaggcgtttc tcgctgtcct ccgcggggca gacgatgagg gactgaatcc gaaggattat
      421 catctggcga gggttgagtc cctggtcagg caatggcgca atggggaacg gcaggtcgag
      481 aagcgcatcg acctcgacct ccttctgacg gacgccttcg tcgcttttgg acgccatgtc
      541 aggagcggcc gcgtagatcc ccgaatggtc tacccagact ggtataactt tcctcgacga
      601 gaaaacggcg acggagaaat cctcaaggtc ttaaaacagg atgagctggg aaaggcgttg
      661 aaggtcattc agccggccga ttcggggtac gaaagactcc gggaagctct ctttcggtac
      721 cggaacatag aaagacgggg tggatggccg tccgtgcccg gcggacctaa gcttaaggct
      781 ggtgaccggg acgaccgggt ccttttgctc cggaagcgtc tgtccctttc cggtgacctt
      841 gatgatggag cggagggagg aagtctcttt gacgaaaagc ttgaccgggc gcttcggcgg
      901 ttccagacga ggcatggcct cgttgcggac ggtgtcctcg gcgcgtcaac tgtagaagag
      961 ctcaatatcc ctgtcgagaa gagggtcagg cagctcaagc tcaaccttga acggctgcgg
     1021 tggctgcccc gggtgctcgg cgaccgatac attttcgtga atttggccga tttcagcctg
     1081 gaagtcgtag aaaaggatga gaccgttatg acaatgcgca tcatcgtggg ccgggacgaa
     1141 gacgaccaac gtaccttagt cttcacgagt aacatcactt acctggaaat caaccccttc
     1201 tggaatgtgc cggagagcat cgccgtgaat gaaatcatcc cgaaggtgaa ggatgatccg
     1261 cattacctgt cttcccgggg catccggatt atcgacggtt ggagcgaacc ggcgaaggag
     1321 gttgacccgg aaagcataga ctgggaatcc ctcgaccccg gacaattccg ttaccggtta
     1381 cgtcaggatc ccgggccgcg gaaccctctt gggcgggtca aattcatgtt ccccaatgaa
     1441 ttcaacatct atcttcacga tacccccgac cggcaacttt tcgagaggga aaggagggcc
     1501 ttcagccacg gttgcatacg tatagaaaag cccctcgacc ttgctgccta tctcctgaaa
     1561 ggttactccg actggaaaag ggaagacatc cttgaggcgg tagcttcgcg gcaaaggcag
     1621 gtggtcaccc tggccgagcc ggtcccggtg tacattttct acctaacggt atgggtggac
     1681 cggaacggcc tcgtccattt ttgccgggat gtttacaacg gcgacaggat gctggagcag
     1741 gcaatgaact ataaaccggt gcggagagcg catctgcccg cccccgcaaa atttccaggc
     1801 tgagggtgtc gttagttttc ttgacaaaat cgtaaaatta cggtttaaag cggcaaagtt
     1861 tctattgagg ttctgtcggt ccgaagatga agaatacgag atcatatcag tccccttcct
     1921 tcaacaaata cccgtccagc tacatttacc tgaaacattc tcacctgaaa ttcgccgcca
     1981 tcggctttgc agtgttgctg ctcagcaatg cagccctcgt ttatttgttg attcggggag
     2041 agatgaagaa tcgggcgttg atggatctgg tcgcccacac aggtcaacgg ttggaagcga
     2101 tcgacaaatc cttgggagaa aaggacctgc agaaggcctt cggtgaactc caggaggtta
     2161 aaaagcaggt cgccgtggtg ttgaagccac agccacaggt cgagaagccc gtcgtgcccg
     2221 tcgccgcggt ggaagcgccg gcacagaaaa aggtccttcc tccacaggct tcggtttcca
     2281 caccggacct ttctcaggag accccctatc cattcgttta tgcgggagag ggggaaaatg
     2341 cgctcatctg tgaaaaggaa acgaagatcc tctatgtttt ccgaaaaagc gggggtaagt
     2401 ggacccttgt caaggcttat ccctgcctga ccggcgccaa ccagcaggac aagagaaaaa
     2461 acggcgactt agcgaccccc ctgggcacgt atctctttct tcggttcatt cccgggaagg
     2521 acctcgcgga gaattatggg ttcggggctt atgtcctcaa ctaccccaat ttcaccgacc
     2581 gaagagaggg acgggacggg acgggaatct ggcttcacgg tcacagcgca gggaagagcc
     2641 tcggcgatca aatcgtgaat acgaagggat gcatcgtggt gagtaacgac gccctgaaag
     2701 aaatgagcca gtacctcaag ccgaggggaa cggccatagc ggtcgtcaat aagctgtcct
     2761 ataccaaggc ggcctcccag gaggctcttt ctaaagaact tcgtggtttt atcgagtcct
     2821 ggagacgggc ctgggaaagc cgggacgttc agaagtacat gagccattat gcggcggagt
     2881 ttgtgagcgc cgacgggatg agctaccagg ccttcaaaag gcacaaggaa agggtgaaca
     2941 gcgggaagaa gttcatccgc gtgacgacgg aagacctcat gattatgctg ccgcaggagc
     3001 gagagggaaa ggttgcgatt gcacggttca ttcagaaata ccagtcgagc aactttaaga
     3061 cggaatcgaa caaggttctt tatctgaaaa aagggcaggg gggatggcaa atcatcggcg
     3121 aatcctcatt ctaacccttt tagtctttgc gtttatattt ctcatcctct tcattgccga
     3181 taccctgagc agggaaaaga atataacggt ggacaaaggg tcgcccggcg tccaggtaaa
     3241 gcgttttttt gcgggaactc cacaggaagt gacggtgtac tttttgcgtg ggagggaaaa
     3301 ggggccgaca ctgttgatct tcagcggaat tcacggtgat gagaagggag gatatctcgc
     3361 ggctgaccgt tacacaaacc tgaacgtccg gaaagggacc ctggtggtag ttccccgttt
     3421 aaacctcccg gcgatcagaa aagaaagacg ggagggtttt gacggcgaca tgaaccgact
     3481 gtttcatctg cccgacgaca gtcaaaccct gcaagattcg aaggtcgtca acctggccaa
     3541 gtccctgatc agggacgccg actacgtcct caacgtgcac cagggcgacg ggttttattc
     3601 ccctcgatgg gtttcccata agcgtaatcc cctgaagtgg ggacagtgca acgtcatcga
     3661 cgcgtcgagt ttcgacctgc cgaacgggca gaaactcgag ctggagcggt ttgccaaggc
     3721 cgttgcagcg agggcgaacg caaagataga agactgtagg tatcactttc tcgttaacaa
     3781 cactaacacg gcggcggagg agtcacgcca caaggaacag cgcaaatctc tgacttatta
     3841 cgcattatcg cggcataaca aagtcgccct cgggctggag gcgacgaaaa gctgctccct
     3901 gccggaagcg gtcgcttttc ttaccgccgt catcaattcc gtgatagaag aggtgggcat
     3961 ttctgttgtg caacttccct cggagtcgcc agaccacata cgcgctgagt ggcagatggc
     4021 gaagggtgaa taaacaatgt gttacggcac ataaccactc cggccgaaac ggccgacagt
     4081 aagcgggatg catatgacga tgacagatga gaaatacagg ctggccgaaa agttgatgca
     4141 ggaaaagcgt tacgacgaag cggtcgctct ttacgaggag ctggtcagac tcaatccggg
     4201 agaggattct caccgtctgg ccctggcctg ggcctataag gacagcggac gcctcgacga
     4261 ggccgtcgcc cttttcgagg aacttctgga aaaagagctt tcgagaaagc tcttcaccgg
     4321 tttcgccttc gacgagctcg tgaggatcct ccgggaagag aagcaatacg accggatgat
     4381 acaaatctgc gaaagggccg ttgccgcgca gccggacgac gtgagtctcc tctttacctt
     4441 gggggacgcc tatctcgctg gcggcttccc cgtcaaggcc atcgacgcct tcaagaagat
     4501 catcatgatg gacccggacg catcttcatt catgagcctt gggaatgccc aggtcgcagc
     4561 gggcgacttt gaaggggcgg aagaggccta cgagcaggcc gggagcttcg acccttcttc
     4621 cagcgacgtt tactacaacc ggttgggtat ggcctatctc agggcgggct atttcgaccg
     4681 ggccgagata gccttccgga aggccatcga ctgcaacgcc acgcagccga tctactattg
     4741 caacgtgggc gacgccttgg ccatggaagg gcggctagac gaagccgagg cggtgtatga
     4801 ggaggctgcg gcgatcaacc ccgggtcgcg gggtgtctac ctcaaccgtc tcggaaacac
     4861 cctccaccgt gaaaaacgct acctgcaggc gatccgggtt ttcaaaaaag ccatcgaaga
     4921 agaaccgaaa aaccccttct accatatgca ccttgcccgt tcttacgagg cggccggttt
     4981 cccggaggaa gcacggaggg aaaacgaaaa ggctcaatcg ttgaagtagg ttgtcaggcc
     5041 ttgcccgttt ttttgagcat ctcgtgaaag acgcggtaaa aggcaacatg gtcgttgatg
     5101 ccgcgtttca gtatcacacc ggcgctatcg atgttcttcg gattgatgac cagattgacg
     5161 ctcctgtaaa aggcggccat gttgtccatg gtccggtatt tgctgctgat aagtgtaacg
     5221 aacatgcgac ggcgggtgga catggacagt ccctgtaggt aggcgaggac gtcgtttgtt
     5281 tctgggtttc ctgtatcgaa gtattcatcg agaacaacga cgtcgtagac gtgaaaacgc
     5341 atcttcttga gggcgtcacg ggcatccgac gcctcggaga tatgaaagcc aaggctgttc
     5401 agtgccgcac tcaccttctc cctgacctcg ggaaccgtac agcagagaag ggcggtttcc
     5461 gcgttttcct cgatgaaatc gaaaggtctg tccgcggagt catacccgcc cgcttccact
     5521 tcctcgagaa ggctcttttc ctcgtccatg tcgcctcgcc tcagaatgat ttcttcttgc
     5581 cgtaatcctt atcgacagtg agttttccga tttccgtcgt cgcctgtccc ctgctgctct
     5641 tgacggcgtc gataccccga ccgacgatcc ccttgttcga ggcgtacgcc cgggccgtct
     5701 cttcgctgat caggccgtct ttgtacaggt caactatgta gtcgtcgaag gttatcatgc
     5761 cgaagggttt cccggcctgg atgatctcgt agaacgtctt tccctccgat tcgccgtgga
     5821 ggatcgcgtc cttgacgcgc aggttcgtgc ccattatctc gaaggcggcg acgcgccccc
     5881 cgccttcttt tgggagcaac ctctggcaca cgatccaccg gacggtgtcg gccagccgga
     5941 tgcgtatctg gttctcttcc tccgtggtga acatgccgat gatgcggttg atcgtcgagc
     6001 cggcgtcgat ggtgtggagg gtgctgaaga ctaggtgccc cgtttcggcc gcgttcaaac
     6061 ctatctctac cgtttcccgg tcccgcatct ctccgacgag gatgaccttg ggagcctgcc
     6121 ggagggcggc ccgcaacccg ctcgcaaagg tgtcgaagtc ggtccccatc tccctctggt
     6181 tgaaggtcgc cttcttgtgt gtgtgggaga actcgacggg gtcttcgagg gttatgatgt
     6241 ggacggcctg ctcttcgtta atttcgtcga ggatggcggc gagcgacgtg gatttgccgc
     6301 tccctgttgc gcccgtcacc agcacgatgc cgttcttttc cctggctatc tgttgaaaaa
     6361 tcgggggtag gttcagctcc tggcatgtcg ggaccttcga ttcgagtttc ctcaagacta
     6421 tcgagtagtt gccccgctgg gagaagatgt tcacccggaa acgggccttt cccggcagct
     6481 cgtaggagga gtcgcaggaa ccggtgttca gcagggtttc cgttagacga tggtcgccgt
     6541 ttatcaggtt gagcgcgaag atctccgtct gaaacggcgt cagtctggtg acaggagggt
     6601 cgagctgaac gggcgtcaac tgtcccgaag actccacctg gaacggcttg cccacggtga
     6661 tgttcaggtc ggagacattc ttgctcgaat cgagcatctt cgagaggatg tagtcgattt
     6721 ccggttttct cacaggacct cctcgttcat atctccgtga agtcagcagg cggcgtcttc
     6781 aggaaaggcc tgaatcgctg tttgtcgttg cacttcaagt aagcgtcgtc gggagtgatc
     6841 atgcctcgct gcagcaggtc ctggatggcg tcgtcgagca tctgcatccc gaattttttc
     6901 cccgtctgga tcatcgaggg tatctggaag gtcttcgatt ccctgatgag attgcggacg
     6961 gccgagttgg cgatcagtat ctcgagtgcc gccacacggc ccttcttatc ggccctcttg
     7021 aaaagcacct gcgatacaac ggcacggata ccgtcggcca ggttggaacg gatttgcccc
     7081 tgttcatttg ccgggaagac ttcgattatg cggtcgaccg tctttgcggc gttcatcgta
     7141 tgcagtgttc cgaagacgag atgtcccgtc gaagccgctt caaccgccag cgaaatcgtc
     7201 tctatgtcac gcatttctcc taccaggatt atgtccgggt cctcacgcag ggcgccgcgg
     7261 agggcggcgc tgaagctctt ggtgtggagt cccacttcac ggtggttcac gacacaactt
     7321 tgactttgat gtacgaattc gatggggtct tctatcgtga tgatatggtc ttttctggtg
     7381 cggtttgcct cgtcgatgat ggccgcgagg gtcgtcgact ttccgctgcc cgtagggccc
     7441 gtcaccagga cgagccccct cgggagggac gcaagtttcg agacgaccgc cggaagaccc
     7501 agctgggcac aggtcaagat cttggtcggg atctcccgga agacggcgcc gacgccgtac
     7561 ttttgctgga agaaattggc ccggtatcgt gcaagtccgg ggatctcgta agcaaagtcg
     7621 atgtcccccg tctcttcgaa ctgcttgacc ttgtgttccg gcgctatctc atagagcatc
     7681 gccttcaatt cgtcgttgtc gaggacattg tacttcaccc tctccatttc cccgcggatt
     7741 ctcaacacgg gttgctggcc ggcgacgagg tggaggtccg atgccccctg ctcgttcatc
     7801 agttggaaaa aggcatcgat cttggccatg gactttctcc ttcggtaagg tccaccactt
     7861 gttatcaccg tgagcgggta ttttcaaggg gtaatggagc ggcgacactc acaaagaatc
     7921 tttttgcttg
//
LOCUS       DAOTMX010000036         7607 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_8509_length_7607_cov_3.842293, whole genome shotgun sequence.
ACCESSION   DAOTMX010000036 DAOTMX010000000
VERSION     DAOTMX010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 7607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7607
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8509_length_7607_cov_3.842293"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..929
                     /locus_tag="P5573_01850"
     CDS             42..929
                     /locus_tag="P5573_01850"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HRT26839.1"
                     /translation="MTVKEAVEKFVHDGDYLCIGGFGANRIPAAVCHEILRQGRKNMA
                     FAGHTSTHDFQILCAGEVFNRLDVAYIVGLEARGLSVGARRYMESGKVEVSEWTNYSL
                     SVRFKAAAAGVSFYPARNMMGTDTFKYSGAKIIECPFTGTKYTAMPAIYPDVSALHVH
                     EADIYGNCRIRGITVSDLDVARASKHLIITCERLISNEEIRREPSLTVIPYWCVDAVC
                     EVPYGSYPGNMYGEYFSDEDHLREWLKVEENPEEFKKFLDKNIYSCKNHFEYIEKNGG
                     LERLQVLRQKEMMFTGARS"
     gene            948..1718
                     /locus_tag="P5573_01855"
     CDS             948..1718
                     /locus_tag="P5573_01855"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HRT26840.1"
                     /translation="MADYNVMELMICTAARELEDGASVGVGTGAPCAAAMLAQKLYSP
                     SLTILFEAGGAAPLIPEMPISVGDSRTTWKAIMASGMCEIMETACRGLLDYTFLGGAQ
                     IDMYGNLNSTRIGPDHQKPKVRFPGSGGANDFASFCWRMMVMTPQDPKRFAEKCQFIT
                     TPGWLEGGDSRAKSGLPLGAGPYKIITNMAVMDFEPESKRMRIIAINPGYSEKDVQDN
                     CGFELLKAKNIVENKPPTVEELTILREQVDPYRYVIGR"
     gene            complement(1873..2073)
                     /locus_tag="P5573_01860"
     CDS             complement(1873..2073)
                     /locus_tag="P5573_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HRT26841.1"
                     /translation="MKKSTYRDFEATELYCPKCRRAVPVRKRLLLILPEGDKYDYSCV
                     YCGTSVGDKTVRQKDNLRIITN"
     gene            complement(2070..4130)
                     /locus_tag="P5573_01865"
     CDS             complement(2070..4130)
                     /locus_tag="P5573_01865"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HRT26842.1"
                     /translation="MKNSYFFNIYRQGFIRTAVCIPEVRVSDTKFNAEKTVSLAKDAA
                     AGNAILALFPELGISAYSNEDLFHQDALLGGVTEALSYILDETKGLNLIMVVGAPLQV
                     DLGLYNCGVVLYQGKILGVAAKSYLPNYREFYEGRQFKAAAEALSTKVSLCGQEDIPF
                     GANLLFEGRNIRNFSFYVEICEDVWVPIPPSSFAAMAGATVLANLSASNITIGKDEYR
                     HSLAANQSARCIAAYLYAAAGPGESTTDLAWDGHAFIYENGNLLSESERFSRKPQLIL
                     GDIDLDRLAQDRMRLTSFSQNAGTHRERLLDFRRVSFSLDVPEGRILLQREYQRFPYI
                     PADPSKRDHRCYETYNIQVQGLAKRMEATGIRNAVIGVSGGLDSTHALIVTARTMDVL
                     GLPRSNIKAYTMPGFATTSKTYSNALKLMDALSVEANEVDIRPSCRQMFKDIGHPFAE
                     GKEVYDVTFENVQAGERTSHLFRIANFRQGLVIGTGDLSELALGWCTYGVGDHMSHYN
                     VNVSVPKTLIQYVIRWVAQTKLLGDAASEVLMEILETEISPELVPGMNKDQPAQSTEA
                     VIGPYELQDFNNFYATRFGYLPSKIAFLAWHAWRDKETGSWPDVPIHKRKSYSLAEIK
                     HWLKVYLYRFFQTSQFKRSCIPNGPKVGSGGSLSPRGDYRAPSDSEATVWMRNAEEIP
                     DEDS"
     gene            complement(4134..4751)
                     /locus_tag="P5573_01870"
     CDS             complement(4134..4751)
                     /locus_tag="P5573_01870"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HRT26843.1"
                     /translation="MNRPFHATKFRTHFNARTIPRHPKIDELKKWCADFHRSGLTPLH
                     KHGACGNLSFRLSDGEIPFVITASGASFHETPQDDFFVKVDHCDMENMVVYAEGTREP
                     SSESLFHFAVYRERKDVNAVFHGHHKLLLLKGPQIGLKETTREEPYGTVELVDSILEV
                     LNQENFLLLKNHGFISLGRTMEEAGELALLMLKRVSPGISEAGKS"
     gene            4902..6443
                     /locus_tag="P5573_01875"
     CDS             4902..6443
                     /locus_tag="P5573_01875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HRT26844.1"
                     /translation="MTDKDTHRKSFQPRQRECPLFIAPALDILRDAVENFRRNGDTNQ
                     AAAIAFYAILSIIPLYILTLLAAGLVFGGSQVETQERLLRLIQEFHPYFSEKLVSQFG
                     QAEQISQKSKVLGWVGVISLIWFSAMVFNALETALDIIFRSKKGRNYFLSKLLAIAMI
                     PAGWVVGIVSVTITYVSTIVAKHPLVMEGGLFAVVTVHNLLFRYVVPYLILVFFSAFV
                     YKVIPTGQVKLSSAVAGGALFSAMMEAAKHLFTWYVENYTRYDVVYGSLGTVVVVVIW
                     IFYVAFMLLFCAELISSYQRRDLILLEKAFVEEKRRKSVNTDERLFKKFGKIYPRGSY
                     IFQEGDADGEMFYILSGCVHVEKRVGQGQKLLAELGPGSYFGEMSPLLEEPRTASVRA
                     FEDSSIALITKDNFRNLIRESVDVSLVMLQEFSRRVRNTNEKLERLAEAWSRMVIVFY
                     FLKEWPIDSGRDPVAELAGLTGKERPEIQELLQNMAKQGIFSLEKDRVVDFNRSRAWE
                     LSLKD"
     gene            6483..>7607
                     /locus_tag="P5573_01880"
     CDS             6483..>7607
                     /locus_tag="P5573_01880"
                     /inference="COORDINATES: protein motif:HMM:NF025499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HRT26845.1"
                     /translation="MMIEAMGMTKDKVLEQIRLLQVGTIPVRLNRPCTVNDGIKVLTE
                     KEQEECVRAHDEAVLSGKVMKFVPASGAASRMFQEWCLAFQQGGFRSRKQAVEFAIAL
                     KKFAFFKDLQSALHRNGMDFDGLVKEGRYVEILAHILTDKGLNYASLPKALLKFHVYG
                     DKSRTALEEHLVEAVLYTRDGHNNCRMHVTVSPEHERLVKVHLSDVLPAYQAQYGVRL
                     IVEVSVQQASTNTITVDLDNRPVRDQRGNLVFRPGGHGALLYNLYATDGDVIMIKNID
                     NVVPDRLKEKTVYYKKVLGGCLILIQKNAFRYAEALSGTGAGGVIPGAERFLSETFHA
                     APPPGYSTLAEREKIDYLFDKLNRPIRVCGMVRNEGEPGGG"
BASE COUNT         1777 a         1861 c         2142 g         1827 t
ORIGIN      
        1 ccttttttcg caaaaaaaat cgtaaattgg taagcaaact gatgaccgtc aaggaagccg
       61 tggaaaagtt cgttcacgac ggggattatc tttgcatcgg cggtttcggt gctaacagga
      121 ttccggctgc cgtgtgtcac gagattctcc gccagggcag gaagaacatg gcctttgcag
      181 gccacacatc aacccatgac tttcagattt tgtgcgccgg tgaagtcttc aacaggcttg
      241 acgttgcgta tatcgtcggc ttggaagcga gagggttgtc agtgggtgcg agaaggtaca
      301 tggagagcgg caaggtagag gtatccgaat ggactaacta cagcttgtcg gtgcgcttta
      361 aggctgctgc ggcaggtgtg tctttctatc ccgccagaaa catgatgggg acggatacct
      421 ttaagtacag cggggcaaag atcatcgaat gtccgttcac ggggacgaag tacacggcaa
      481 tgccggccat ctatcctgat gtgtcggcgc tccatgttca tgaggcggac atctacggga
      541 actgccgcat aaggggtatc actgtgtccg acctcgatgt cgcccgggcg tctaaacacc
      601 taatcatcac ctgtgagagg ttgatttcga acgaagagat ccgcagagaa cccagtctga
      661 ccgtcattcc gtactggtgc gtcgacgcag tctgtgaggt tccttacggg agttacccgg
      721 ggaatatgta tggcgaatac ttctctgacg aagaccatct gcgggaatgg ctcaaggtgg
      781 aagagaatcc ggaagaattc aagaaattcc tggataagaa catctacagc tgcaagaacc
      841 actttgagta catcgagaag aacggtggcc tggagaggtt acaggtgctg cgacaaaaag
      901 aaatgatgtt taccggtgcc cggtcttaga aaggaggacg taagaacatg gctgattata
      961 acgtaatgga attgatgatc tgcaccgccg caagggaact tgaggatggt gcgtctgtcg
     1021 gtgtcgggac gggggcgccc tgtgcggcgg cgatgcttgc acagaagctc tattcaccga
     1081 gcctgaccat tttatttgaa gcgggtggtg ccgcccccct gattcctgag atgccgatct
     1141 ccgtgggaga ctccaggaca acctggaagg cgatcatggc gagcgggatg tgtgagatca
     1201 tggaaacggc ctgcaggggg cttttggact acacattcct tggcggtgcc cagatcgata
     1261 tgtacggaaa cctgaactcg accagaatcg gtcctgacca tcagaaaccg aaggtccgtt
     1321 tcccgggaag cggcggcgcg aatgacttcg cctcattctg ctggaggatg atggtcatga
     1381 caccacagga tccgaagaga tttgcggaaa aatgtcagtt cataaccacg ccgggatggc
     1441 tcgaaggcgg agattcccgg gcgaaatctg gactgccact cggagccggg ccctacaaga
     1501 tcatcaccaa catggccgtc atggattttg aacccgagtc gaagagaatg aggatcatcg
     1561 ccatcaatcc cggctactcg gaaaaagacg ttcaggacaa ctgcggattt gagcttctca
     1621 aggccaagaa tatcgtcgag aataaaccgc cgacggtgga agagctgacg atcctccgtg
     1681 aacaggttga cccctatcgt tacgtcatcg gaagataact gaaatatggc gcatgagaaa
     1741 gccgcggtcg tcaggccgcg gcttttttgt gtgactgaac ctaagatgac accccaggtt
     1801 tagagaagag ctcgaagtaa catctgtcgg agtgtgtcat tgctcgacgg ttttgccgag
     1861 atgccgatat cgtcagttcg tgatgatccg gaggttgtcc ttttgtctga ctgttttgtc
     1921 ccccaccgaa gtgccgcagt agacacaaga gtaatcgtac ttatccccct ccggcaggat
     1981 gagaaggagg cgtttcctca cgggaacggc ccgtctgcac ttggggcagt ataattccgt
     2041 cgcttcaaag tctctgtagg tcgacttttt catgaatcct catcgggtat ttcttcggca
     2101 tttctcatcc agacggtcgc ctcactgtcg ctcggtgccc tgtagtctcc acggggcgac
     2161 aacgaccccc ccgaccccac cttggggccg ttagggatgc aggaccgctt gaactgactg
     2221 gtctggaaaa aacggtagag gtacacttta agccagtgtt ttatctctgc taaactataa
     2281 gattttctct tgtggatagg aacgtcgggc caggacccgg tttctttgtc ccgccaggcg
     2341 tgccaggcca ggaaggcgat cttagaaggc agatagccga accgagttgc gtagaagttg
     2401 ttgaagtcct gcaattcgta aggaccgatc accgcctccg tagactgtgc cggttggtct
     2461 ttgttcatgc cgggtacgag ctccgggctt atctccgttt ccagtatttc catgaggacc
     2521 tcagacgcag catcgcccag caattttgtt tgtgcgaccc agcggatgac gtactggata
     2581 agagtcttgg gaacgcttac gttaacgttg taatgagaca tatggtctcc tacaccatag
     2641 gtgcaccagc cgagggccag ttcgctcaag tcgcccgtcc cgatgacgag tccctgccga
     2701 aaattggcta tccgaaatag gtgggaagtc cgctcaccgg cctggacatt ctcaaaagtg
     2761 acgtcgtaaa cttccttgcc ttcggcgaag ggatgtccga tgtccttgaa catctgccta
     2821 caactcggcc ggatgtcgac ttcgttcgct tcgacgctca gggcgtccat cagctttaat
     2881 gcgttactgt aagtcttgga cgtagtggcg aatccgggca tcgtgtaagc cttgatgttt
     2941 gaacggggaa gtcccaagac atccatcgtg cgagcagtta cgatgagggc gtgagtggaa
     3001 tcgaggccgc cggaaacgcc gataacggca ttgcgtatac cggtggcttc catgcgcttg
     3061 gctaacccct ggacctggat gttgtaagtc tcataacagc gatggtccct cttggacggg
     3121 tcggcgggga tgtaggggaa tctctggtat tcccgctgaa ggagaattct tccctcggga
     3181 acgtctagag agaaggatac cctacggaaa tcgagcagcc tctccctgtg agttccggca
     3241 ttttgactga agctcgtgag acgcatccgg tcctgggcaa gacggtcaag gtcgatgtcg
     3301 ccgaggataa gctgcggttt gcgcgagaat cgctccgact cggaaaggag gttaccgttc
     3361 tcatagataa aggcgtggcc gtcccaggcg aggtcagtcg tcgactctcc ggggccggct
     3421 gcggcgtaaa gataggcggc gatacagcgg gcggactggt tggcggcaag actgtggcgg
     3481 tactcgtctt tcccgatagt gatgttcgac gctgacaggt tcgccaagac cgtcgctcct
     3541 gccatggcgg cgaaactcga aggggggatc gggacccaga cgtcttcaca gatctctaca
     3601 tagaacgaaa aattacggat gttgcgccct tcgaacagga ggttcgcgcc gaaggggatg
     3661 tcttcttgac cgcaaaggct cactttcgtc gataaagcct ccgccgccgc tttgaactgc
     3721 cggccttcgt agaattccct gtaattagga aggtacgact ttgccgcgac acccaagatt
     3781 ttgccttggt acaggacaac cccgcaatta tagagtccca ggtcgacctg gagaggcgca
     3841 ccgacgacca tgattaagtt gaggcctttg gtctcatcca ggatgtagct cagggcctca
     3901 gtcacgcctc caagaagggc atcctggtgg aagaggtctt cgttggaata ggcggatatt
     3961 cccaattcag ggaagagggc gaggatggcg ttcccggccg ctgcatcttt cgcgaggctg
     4021 accgtctttt cggcgttgaa tttcgtgtcg gagacccgga cctccggtat gcacacggcc
     4081 gttcggatga acccctggcg gtagatattg aaaaaatagc tgtttttcat gtcttagctc
     4141 ttccctgcct cagatatgcc gggtgaaact ctcttcaaca tcagaagcgc cagctcacct
     4201 gcttcctcca tcgttcggcc gagcgaaatg aaaccgtgat ttttgaggag caaaaagttt
     4261 tcctgattca ggacctcgag gatactgtca acgagttcaa cagttccgta aggttcttcc
     4321 ctcgtcgttt ccttcaagcc tatttgcgga cctttcagta gaagaagttt gtgatgacca
     4381 tgaaaaacgg cgttgacatc ttttctttcg cggtaaaccg caaagtgaaa cagactctcc
     4441 gaggatggtt ctcttgtgcc ctcggcgtaa acaaccatgt tctccatgtc gcagtggtcc
     4501 accttcacaa aaaaatcatc ttggggcgtc tcatggaaag aggccccgga ggcagttatc
     4561 acgaacggga tttcaccgtc tgataaccta aagctcaagt tgccacaggc gccgtgcttg
     4621 tgcaaaggtg taaggccaga acggtggaaa tccgcgcacc acttcttcaa ctcatcgatt
     4681 ttcggatgtc ttggtatggt ccttgcgttg aaatgggttc gaaattttgt cgcgtgaaaa
     4741 ggacggttca tggtttcttt gaagtattta cttaaaggag cattctttgt caaagaaaaa
     4801 aaggtgtcga accaatatcc ttgacaccgg cgggttactt ccctacactc gtcaatcatc
     4861 gagccctcat cactgtatga aaaacttggc cgctgccacc catgactgat aaggataccc
     4921 atcggaaaag ctttcagcca cggcagagag aatgcccgtt attcatcgcc cccgccctgg
     4981 atatccttag ggatgcggtc gaaaacttcc gcaggaacgg cgacaccaat caggcggcgg
     5041 cgatagcctt ctacgccatt ctttccataa ttcccttgta catcctgaca ctccttgcag
     5101 ccggtttggt tttcggcggt tcgcaggtgg agacccagga gagactcctc cgtctcatcc
     5161 aggaatttca cccctatttt tcggaaaaac tcgtctccca attcggtcag gcggaacaga
     5221 tatcacagaa aagcaaggtc ctcggctggg tgggagtcat ttctctcatc tggttttcgg
     5281 ctatggtctt taacgccctt gagacggccc tggacatcat ctttcgttcc aagaagggca
     5341 ggaattactt tctttccaaa ctgctggcca tagccatgat tcctgcgggc tgggttgtgg
     5401 gcatagtcag cgtcacgatc acctacgttt cgactattgt agccaaacat ccgctggtta
     5461 tggaaggggg cctttttgca gtcgttaccg ttcataactt gctgttccgg tacgtcgttc
     5521 cctatctgat actcgtgttt ttctctgctt ttgtttacaa ggtcataccc acaggtcagg
     5581 tcaaactttc cagtgccgtt gcagggggtg ctctgttttc agctatgatg gaagcggcga
     5641 agcatctttt cacctggtat gtggagaatt acacgaggta tgacgtcgtc tatgggtccc
     5701 ttggtacggt ggtggttgtg gtgatctgga ttttctacgt tgccttcatg ctgcttttct
     5761 gtgcagaact catttcttcc tatcagagac gtgacctgat acttctcgaa aaggcgtttg
     5821 tagaggagaa gaggagaaaa agcgtcaata cagacgaacg gcttttcaaa aagttcggga
     5881 agatctatcc tcgcggttct tacatctttc aagaggggga tgccgacggg gagatgttct
     5941 atatcttatc cgggtgtgtt cacgtggaga aacgggtcgg tcaggggcaa aaactcctcg
     6001 ctgaactcgg accgggatcc tatttcgggg agatgtctcc gctcctcgag gagccaagaa
     6061 ctgcgtccgt cagggcgttc gaagacagca gtatcgcctt gataacaaaa gataattttc
     6121 gtaacttgat cagggaaagc gtcgatgtat ctctggtcat gctccaagaa ttttcccgtc
     6181 gagtaagaaa tacgaacgaa aaactcgagc ggttggctga agcctggagc aggatggtca
     6241 ttgtgttcta ttttctcaaa gaatggccga tagactccgg ccgagacccg gtggctgaac
     6301 tggccgggct gacgggcaag gagaggccgg agatccaaga actcttgcag aacatggcga
     6361 agcagggcat ctttagcctg gaaaaagacc gtgtcgttga tttcaaccgc agtcgggcct
     6421 gggagctttc cttgaaggat tagaagagta tggaagggat taccttatca gaccgggatg
     6481 ttatgatgat tgaggcaatg ggtatgacga aggacaaggt tctcgaacag attcgcttgc
     6541 tccaggtcgg gaccatccct gttcgtctga acaggccatg cactgtaaat gacggaatca
     6601 aggtcctcac tgaaaaggaa caggaagaat gtgtaagggc ccacgatgag gccgtccttt
     6661 cgggcaaagt catgaagttt gtgcccgcct caggggccgc cagcaggatg tttcaggaat
     6721 ggtgtttggc attccagcag ggcggtttta ggtcgcggaa acaagcagtg gaatttgcga
     6781 tagccttgaa gaagttcgcc ttttttaaag atttgcagtc cgctcttcat agaaatggca
     6841 tggatttcga cgggttggtg aaggagggga ggtatgtcga gatccttgcc catatcctga
     6901 ccgataaggg actgaattac gcgtctcttc ccaaggctct ccttaaattc catgtgtacg
     6961 gagacaaaag tcgcactgcg ctcgaggagc atcttgttga agcggtactc tatacacggg
     7021 acggccataa caactgtcgt atgcatgtga cggtgtcgcc ggagcacgaa agactggtta
     7081 aagttcatct ttcagatgtg ctcccggcct atcaggctca gtacggagtg agattgatag
     7141 ttgaagtgtc ggtgcagcag gcatcaacga acactataac ggttgacctg gacaaccgtc
     7201 cggtgaggga tcaaaggggg aaccttgtgt tccgccccgg cggccatggc gcgcttttgt
     7261 acaatctcta cgccacggac ggtgatgtga tcatgatcaa gaatatcgac aacgttgttc
     7321 ccgaccggtt gaaagagaag acggtctatt acaagaaagt tctgggaggg tgccttatct
     7381 tgattcagaa gaatgctttt cgatatgccg aagccctgtc cggtactgga gccggcgggg
     7441 tgattccggg tgcggaaagg tttttgagcg agacattcca tgcagctcct cctcccgggt
     7501 acagtaccct ggcagaaagg gaaaagatcg attacctttt cgataaattg aatcgtccga
     7561 tccgtgtctg cggtatggtc aggaatgaag gggagccggg aggaggt
//
LOCUS       DAOTMX010000037         7590 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_8530_length_7590_cov_3.604778, whole genome shotgun sequence.
ACCESSION   DAOTMX010000037 DAOTMX010000000
VERSION     DAOTMX010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 7590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7590
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8530_length_7590_cov_3.604778"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /gene="aroC"
                     /locus_tag="P5573_01885"
     CDS             complement(<1..537)
                     /gene="aroC"
                     /locus_tag="P5573_01885"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HRT26846.1"
                     /translation="MSGNTIGTVFRVTTWGESHGPSVGAVVDGCPPGVDLDESDIQQE
                     LDRRKPGARAFASPRKEEDRVEILSGTFEGKTTGTPIALLIRNRDSDSAAYDELRNLF
                     RPGHGDFTYFKKYGIRDHRGGGRASGRETAARVAAGAVANKVISREGISVLAFAKEVG
                     GVAAKKAPPGDMAENPLLC"
     gene            complement(627..1184)
                     /locus_tag="P5573_01890"
     CDS             complement(627..1184)
                     /locus_tag="P5573_01890"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HRT26847.1"
                     /translation="MNEQTDGANRNIILIGYRSAGKTAVGGRLAERLGLPFYDTDDLI
                     CQRKGKSVSEIVAEMGWAGFRKEERIVIKSLTFVTGCIIALGGGAVMDPLNLEVLKAH
                     GVFIWLDAAPTAIVERMEKDAATAAQRPPLSGTDSLGETAAMLKEREPIYRLLANYRI
                     DTTGRGVDEVVDEISGLIQEKNLLF"
     gene            complement(1190..2470)
                     /gene="aroA"
                     /locus_tag="P5573_01895"
     CDS             complement(1190..2470)
                     /gene="aroA"
                     /locus_tag="P5573_01895"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HRT26848.1"
                     /translation="MKEMKEIEIKPVSRVDAAVKIPGSKSYTQRALAIAALAEGTSFL
                     SNALFSGDTERLMSALRDLGANILVGDDGITVTGTGGNILNPDREIHLGNNGTALRLL
                     ISLVALGRGNYVLTGDNRLCERPVKPLLDALASMGVRARSDGGFPPVTIDAEGLLGGT
                     VALRDIESSQYVSSLLISAPYAAKDTTVELRGRTPSQPYIDMTLETMEAFGVPVAREA
                     PGRYGVKAGRHYRGRRYRVEGDASSASYFFLAAAVSGGRVRVEEVNPRTLQGDIAFLG
                     ILEQLGCAVLRGNRLVEVKGGILRAGDFVFDLGDMPDMVPTLAVLGAVRPGKTVVKNV
                     SHLRFKESNRLEALVTELNRVGIRADETEDGLVIEGGNPRGAAIETYNDHRIAMSFAV
                     LGLVVPGMRIKDGDCVKKSFPGFWAELSKLTSYS"
     gene            complement(2481..3317)
                     /locus_tag="P5573_01900"
     CDS             complement(2481..3317)
                     /locus_tag="P5573_01900"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF001319.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HRT26849.1"
                     /translation="MNRKEEATRVFGLLGNPVGHSLSPLMHNAAYREMQLDAVYAPFC
                     VNDLEGAIRGIKALDIRGVSVTIPFKTAVLPLLDDIEESARTIGAVNTIVNDCGHLTG
                     FNTDWTGLVRSLEELTNIRGKTFAVLGAGGTARAAVFGIRERGGVPVVVNRSEERGRR
                     LALEFGCRFFRLDEIEKVEADCLINTTPVGMAPQEEAMPVDRRNISRFRWVVDVIYRP
                     WRTRLLEEAEKSGAGTMNGAGMLVYQGAEQIHLWTGQEPPVALMRRVVREQLKAEKTG
                     DL"
     gene            complement(3327..4010)
                     /gene="aroD"
                     /locus_tag="P5573_01905"
     CDS             complement(3327..4010)
                     /gene="aroD"
                     /locus_tag="P5573_01905"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF013640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HRT26850.1"
                     /translation="MRICVSIMAGSSDEALAKMETALTRADLVELRVDRIKNPDLGRL
                     LAGRNKKVVVTNRRREEGGSFQGTENERMELLREAVCLGAGYVDVEAATGPLHIRELA
                     EMIGDRGVTKLIISHHNQTGTPGEGVLQKTIGVCRSLGADIVKVVTTARTPEDNLKVL
                     RLIPYARRKGLEIIAFCMGDKGRISRVASPILGSFLSFASLERGEESAPGQMTVDEMR
                     LVLSLLYTA"
     gene            complement(4007..5086)
                     /gene="pheA"
                     /locus_tag="P5573_01910"
     CDS             complement(4007..5086)
                     /gene="pheA"
                     /locus_tag="P5573_01910"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HRT26851.1"
                     /translation="MRKKTLEELRDELRRKDLEIVELLNERARISVEVGRVKGAEGCE
                     VYDPAQESRVYEQIKKVNQGPLTDYALKSIFREILSSSRALQKKEVVVYLGPEASFSH
                     LAALSHFGKSADLVPAVSIPDVFKAVEGEKADCGVVPVENSVEGSVKQTLDRLVVTPL
                     TVRAEIFQRISHCLLSPGVDLSAVRRVYSHPQALAQCQGWLRAHLPDRPLMEMPSTAA
                     AARRVLEEKEGAAIGSALAAEVYGLNVLVQGIEDEPLNTTRFLVVGRGRNTATGMDKT
                     SIVFGTPHVPGALYSVLEPIARAKLNMLRIESYPLRDRMWEYLFFVDLAGHDEDEKIV
                     DCIKEMEKRATFIKVLGSYPRGNGQ"
     gene            complement(5083..6174)
                     /gene="aroB"
                     /locus_tag="P5573_01915"
     CDS             complement(5083..6174)
                     /gene="aroB"
                     /locus_tag="P5573_01915"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HRT26852.1"
                     /translation="MNRIRVNLDKKVSKSYDICIGRAIMDRTALILAKNNWASRYVII
                     TDSTVEELHGRRVLKIFDDANLKADMISFPAGESAKTLETGLRIAEKLLDLGVDRGSL
                     VIALGGGVVGDLAGFVASVYMRGIPYVQIPTTLLAQVDSSIGGKTGVDLPAGKNFLGT
                     FYQPKGVFIDLTFLETLPAREFGNGLAEIMKYGVIDDPELLEALEGGVDALRTDDTLL
                     ERVVARSCHIKKGIVEIDETEKGLRRVLNFGHTVGHAVEAESGYRMSHGESVAVGMAA
                     AASISEKLKYLPAEDRGRIAALIRTIGLPDRLPQDMSTEGILSRIKNDKKKDGDTVHF
                     VLLKKLGLPFMNGGVPEKLVREVIEEMKG"
     gene            complement(6171..7199)
                     /gene="aroF"
                     /locus_tag="P5573_01920"
     CDS             complement(6171..7199)
                     /gene="aroF"
                     /locus_tag="P5573_01920"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HRT26853.1"
                     /translation="MVIVMKRNATEKQVEQVIAWIESVGYTAHPSQGVERTIIGAVGD
                     SRDKAQLKHIEHLPGVEKVVPILKPYKLASREAREGNTIVRVGDAAVGGPELVVMAGP
                     CSIESEEQLMESAYVVKKGGAQILRGGAYKPRTSPYSFQGMEEEGLKLLKLVRERTGM
                     PVVTEVMNTTDVDLVEAHADVLQVGARNVQNFALLKKVGQARKPVLLKRGMMTTIEEL
                     LMSAEYILSAGNHQVILCERGIRTFETATRNTLDLSAVPVLKEATHLPVIVDPSHAAG
                     HWRLVAPLARAAVAAGADGLLIEVHPEPEKAFSDGLQSLKPEKFYQLMDDLRTLERVM
                     KTELGAQS"
BASE COUNT         1423 a         2331 c         2069 g         1767 t
ORIGIN      
        1 gcagagcagg gggttttccg ccatgtcccc cggaggggcc ttctttgcgg caactccgcc
       61 cacctctttc gcgaaggcga gcacagagat tccctcgcgg gagatgacct tattagctac
      121 cgctcctgcg gccacgcgcg ccgcggtttc ccgtcccgag gcccggcccc ctccccgatg
      181 atcccggatg ccgtatttct tgaaataggt gaaatctcca tgaccgggcc tgaaaaggtt
      241 gcgtaattca tcgtaagcgg cgctgtcgct gtcgcggttg cggatgagga gagctatagg
      301 cgtcccggtt gtctttcctt cgaaggtccc ggaaagtatc tcgacgcggt cctcttcctt
      361 cctcggcgag gcgaaggccc tcgcgccggg tttgcggcgg tccagctcct gttggatgtc
      421 gctctcgtcg aggtcgaccc cgggaggaca gccgtcgacc acggccccga cggacggtcc
      481 gtgggattcc ccccaggttg tgacgcggaa aacggttccg atcgtgttac cggacataga
      541 ctttcctctt ttttaatttt tcgaccgtgt ccttaatcct tcgggatcgg ctcgaccccc
      601 catcattttt aagcaagcaa aggcttctaa aaaagtaggt ttttctcttg gatcagtccg
      661 cttatctcgt caactacctc gtcgaccccg cggccggtcg tgtctatccg gtagttggcc
      721 aatagacggt agatcggttc ccgctctttg agcatagcgg ccgtctcgcc cagcgaatcc
      781 gtacccgata gaggggggcg ctgggcggcc gtcgcggcgt ctttctccat tctttcgacg
      841 attgccgtgg gggctgcgtc cagccagatg aaaaccccat gtgccttcag gacttccagg
      901 tttaaggggt ccatcacggc gcccccaccg agggcgatga tgcagccggt gacgaaggtc
      961 agactcttga tgacgatcct ttcttccttg cggaagccag cccaccccat ctcggcgacg
     1021 atctcgctga cacttttccc ctttcgctga cagattaggt cgtccgtgtc gtagaaagga
     1081 agtccaagcc tctccgcaag acggccgccg actgcggttt tccccgccga ccggtaaccg
     1141 atcaggatga tattcctgtt tgcgccatca gtttgttcat tcatctcgat cagctataag
     1201 aggtcagctt gctcaattcc gcccagaacc cggggaacga cttcttgacg cagtccccgt
     1261 ctttgatccg catccccgga acgacaagcc ccagtacggc aaagctcatg gcgatccggt
     1321 ggtcgttgta cgtctctata gcagcgcccc gtgggtttcc gccttcaatc acgaggccgt
     1381 cctctgtctc gtcggcccgg ataccgaccc ggttcaactc tgtaacgagc gcctccagac
     1441 ggttgctttc tttgaacctc aggtgagaca catttttgac gaccgtcttt cccggccgca
     1501 cggccccgag aactgcgagg gtagggacca tgtcgggcat atcgccaagg tcgaagacga
     1561 aatccccagc cctcaatatt ccgcctttta cctctacgag gcggtttccc ctcaggacgg
     1621 cacagccgag ctgttccagg atacccagaa aagcgatgtc tccctggagt gtcctgggat
     1681 tcacttcttc cacacggacc ctacccccgc tgacggcagc cgccaagaag aagtaagaag
     1741 cgctagaggc gtctccctcc acccggtaac gcctgcccct ataatgcctt cccgctttta
     1801 ccccgtatcg gccgggcgcc tctctggcga ccgggacgcc gaaggcctcc atcgtctcga
     1861 gggtcatgtc gatgtaaggt tgagaaggcg tgcgcccccg gagttccacg gtcgtgtcct
     1921 ttgctgcgta aggggcgctg atcaaaagcg acgagacgta ctgactgctt tcgatgtcgc
     1981 gaagagcgac tgttccgccc agcaggcctt cggcgtcgat ggtgaccggg ggaaaaccgc
     2041 cgtcgctccg ggcacggaca cccatcgacg ccagggcgtc gagcaggggc ttgacgggcc
     2101 tctcgcacag gcggttgtct ccagtgagga catagttgcc tcggccgagc gcgacgagag
     2161 aaattaagag tcgcaaggcc gttccgttgt taccgagatg gatttccctg tcggggttga
     2221 gaatattgcc gcccgtgccg gtgactgtga tgccgtcatc tccgacgagg atattcgcgc
     2281 cgagatcacg cagggccgac atcagccgct ccgtgtcgcc ggagaagagg gcattactca
     2341 ggaacgaggt cccctccgcc agggcggcaa ttgcaagcgc gcgctgggta taacttttcg
     2401 acccggggat cttcacggcg gcgtcgacac ggctcacagg ttttatttcg atctctttca
     2461 tttccttcat acgacgatgg ttaaaggtct cccgtcttct cggccttgag ctgttcccgg
     2521 acgacccggc gcatcagcgc caccggcggt tcctgccccg tccagaggtg gatttgctcc
     2581 gccccctggt acacgagcat ccccgcgccg ttcatcgtcc cggcgccgga tttttcggcc
     2641 tcttcgagaa gcctcgttcg ccagggtcgg tagatgacgt cgacaaccca cctgaagcgg
     2701 gagatgttgc gcctgtcgac cggcatcgcc tcctcctggg gtgccatccc caccggtgtc
     2761 gtgttgatga ggcagtctgc ctccaccttt tctatctcgt cgagacggaa gaatcggcaa
     2821 ccgaattcaa gggccaatcg acgtccccgt tcttccgaac gattcacaac gacgggaacg
     2881 ccgcccctct cccggatacc gaagacggcg gccctggcgg tcccgcctgc tccgagtacg
     2941 gcgaaggttt tgcccctaat gttcgtcaat tcctccaaac tccggacgag cccggtccag
     3001 tcggtgttga agccggttag gtgtccgcag tcgttgacga tagtgttcac ggcgcctata
     3061 gtacgggcgg attcttctat gtcgtcgagg agcggtagga ccgccgtctt gaaggggatc
     3121 gtaacactca cgcctcttat gtcaagcgcc tttatcccgc ggatcgcccc ctccaggtca
     3181 ttcacacaga agggggcgta gacggcatcg agttgcatct ccctgtaagc ggcgttgtgc
     3241 atcaggggcg acaagctatg tcctacaggg ttacccaaca acccgaacac cctggtggcc
     3301 tcttctttcc tgttcaaggt gcgcctctaa gcggtgtaca gcaaactaag gaccagcctc
     3361 atttcatcca ccgtcatctg tcctggcgcc gattcctcgc ccctttcgag tgaggcaaag
     3421 gaaaggaaag agcccaagat gggcgaggct acgcggctga tacgtccttt gtcgcccatg
     3481 cagaaagcga tgatttccag tcctttcctc cgggcatagg ggatgagtct gagtaccttc
     3541 aggttgtcct ccggtgtacg ggccgttgtt acgaccttga cgatgtctgc tccgagggaa
     3601 cggcagacac cgatcgtttt ttgaaggaca ccctcgccgg gtgtacccgt ttggttgtga
     3661 tgcgaaatga tcagttttgt cacaccacga tctccgatca tctccgcaag ttcccgtatg
     3721 tgaagaggcc ctgtagccgc ctcgacgtct acgtaccctg cgcccaggca gacggcttcc
     3781 ctcaggagtt ccatccgctc attttccgta ccctgaaagg agcctccttc ctcccgccgc
     3841 cggtttgtca cgacgacctt tttgtttctt ccggcaagca gtctcccgag gtccgggttc
     3901 ttgatcctgt cgaccctcaa ctccacgagg tccgcccgag taagggccgt ctccatcttc
     3961 gcgagagctt cgtcggagct cccggccatg atggatacgc agattctcat tgaccgttac
     4021 ccctcggata ggaccctaag accttgatga aggtggccct cttttccatc tctttgatgc
     4081 agtcgacgat tttttcgtcc tcgtcgtgcc cggcaaggtc gacgaagaat aggtactccc
     4141 acatacggtc ccgcagcggg taggattcga tccgcagcat gttgagcttc gcccttgcaa
     4201 tgggttcgag gacgctataa agggcgcctg gcacatgcgg tgtgccgaac actatggatg
     4261 tcttatccat gccggttgcc gtgtttctgc cccttccgac aacaagaaac cgcgtcgtgt
     4321 ttagcggttc atcctcgatc ccctgaacaa ggacgttgag gccgtaaacc tcagcggcga
     4381 gggcgcttcc gatagcggca ccttcttttt cttcgaggac acgccgggcg gcggcggccg
     4441 tgctcggcat ttccatcaag ggacggtctg gcagatgggc tctgagccag ccctgacact
     4501 gggcgagggc ctgaggatgg gaatagaccc gtctgacggc actcaggtct actccgggag
     4561 agagaagaca atgggagatc ctctggaaga tttcggccct gacggtaagc ggtgttacca
     4621 ccaggcggtc tagggtctgc ttgacggagc cctcgaccga attctccacg gggacgacgc
     4681 cgcaatcggc tttctccccc tctacagcct tgaatacgtc agggatggaa accgccggca
     4741 ctaaatcggc gctctttccg aagtgggaga gtgcggcaag gtgggaaaaa gacgcctcag
     4801 gcccgagata gacgacgact tccttcttct ggagtgcccg ggaagaagaa aggatttctc
     4861 ggaagatgct cttcagggca tagtcagtca aaggcccctg attcaccttt tttatttgct
     4921 cgtagaccct gctttcttgc gccgggtcgt acacctcgca accttcagca ccctttaccc
     4981 gtccgacttc aacggagatg cgcgccctct cattcaacag ctcaactatc tcgaggtctt
     5041 tcctgcggag ctcgtccctc aactcttcca gagtcttttt cctcaaccct tcatctcctc
     5101 gattacctct cggaccagtt tttctgggac accgccgttc atgaacggca gccccagttt
     5161 tttcagaagg acaaaatgga ccgtgtcgcc gtcctttttc ttatcgttct tgatccgcga
     5221 tagtattcct tccgtgctca tatcctgcgg cagccggtcc gggaggccga ttgtcctgat
     5281 cagcgcggcg attcgccccc ggtcctctgc gggcaggtac ttgagttttt ccgagatcga
     5341 agcggcggcc gccatcccta cagcgacact ctccccatgg gacatcctgt acccggactc
     5401 cgcttccacg gcgtgaccga cggtgtggcc gaaattaagg acgcggcgga gccctttttc
     5461 ggtctcgtcg atttccacga tccccttctt gatatggcag gacctggcga cgactcgttc
     5521 gaggagcgta tcatccgtgc ggagggcgtc tactccgcct tcgagtgctt ccagtagctc
     5581 ggggtcatcg atgacgccgt atttcataat ctccgccaag ccgtttccga attccctggc
     5641 cggaagggtc tccaagaatg tcaggtcgat gaagacacct ttgggctggt agaaggtgcc
     5701 gaggaaattc tttcccgccg gcaggtcaac acccgtcttg ccgcctatac tgctgtctac
     5761 ctgtgccagg agcgtagtcg gtatctgtac ataggggatc cccctcatgt agacggaggc
     5821 cacgaatccg gcgaggtctc ccacgacgcc cccgcccagg gcgatgacca gtgagccccg
     5881 gtcgacgccg aggtccagga gcttctccgc gatgcgcaat cccgtttcca gcgtcttggc
     5941 actctcgccg gccgggaagg agatcatgtc cgctttgagg ttcgcatcgt cgaagatttt
     6001 cagcaccctc cggccgtgaa gttcctccac ggttgaatcg gtgatgatca cgtaacggga
     6061 tgcccagttg ttcttggcga gaatcagggc cgtcctgtcc atgatggccc gccctatgca
     6121 aatatcgtaa gacttcgaga ccttcttgtc gagattgact cttatccggt tcatgattgc
     6181 gcccccaact cagttttcat gactctttcc agtgtccgca ggtcgtccat gagctggtag
     6241 aatttttccg gcttcaggga ctgcaacccg tcggaaaagg ccttctctgg ttccggatgg
     6301 acttcgataa gaaggccgtc ggcgcctgcc gcaacagcag cacgggcgag cggtgcaacc
     6361 agtcgccaat ggcctgcggc gtgactcggg tcgacgatca cggggagatg ggtcgcttcc
     6421 tttagcaccg ggacggcact taggtcaagg gtgttgcgtg tagccgtctc gaaggtcctg
     6481 attccccgct cgcagaggat gacctggtga tttcccgccg agaggatgta ttccgccgac
     6541 atcagaagct cttcgatggt ggtcatcatc ccgcgcttta gtaagacggg ctttcgcgcc
     6601 tgtccaactt tcttcaggag agcgaaattc tgaacattgc gggcgccgac ctgcaggacg
     6661 tccgcatggg cttcgacgag gtcgacgtct gttgtgttca tcacttcggt gactaccggc
     6721 atgccggttc gctcgcggac cagtttcagg agtttcaggc cttcttcttc catcccctgg
     6781 aagctgtacg gcgacgtccg cggtttatag gcccctcctc tcagtatctg ggcgccccct
     6841 ttcttgacta cgtaggcgct ttccatcaac tgttcctcgc tttcgatcga acagggtccc
     6901 gccataacga ctaattccgg cccgccgacg gcggcatcac ctacccggac tatcgtgttt
     6961 ccttcccgcg cctcacggct ggctagcttg taaggtttca gtatgggcac gaccttttcg
     7021 acaccgggca ggtgctcgat gtgcttgagc tgagccttgt ccctgctgtc tccgacggcg
     7081 ccgatgatgg tccgctcaac accctgggac ggatgggcgg tataacctac ggattcgatc
     7141 catgcgatca cttgctctac ttgtttttcg gtcgcgtttc ttttcattac gataaccatt
     7201 tctctcctcc aaaaagaaag gccatggtga tgctgctctc accatggccc gaaataaaaa
     7261 aagccatggg cagcaacttc ggtctgtcca tggctttttt actattttat cgaggtttaa
     7321 gtcctcctga gcgatgggca gaccgatgtg ccgaaataat aatagccgaa ccagaagcgc
     7381 aacatgccat cgaccttctt ttgtctaccc attcagaaat atccaaccta taaacaatgt
     7441 tgacgccata taaacactag accctgcgct tgtcaaggag aaaatttttg cctatcgttc
     7501 attattgcat tacctctaaa aaccgtgtat gtcttacaca atccatcatt ttgacctgtg
     7561 gggcagtaat ataatggaaa aattcgaggt
//
LOCUS       DAOTMX010000038         7580 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_8537_length_7580_cov_3.598272, whole genome shotgun sequence.
ACCESSION   DAOTMX010000038 DAOTMX010000000
VERSION     DAOTMX010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 7580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7580
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8537_length_7580_cov_3.598272"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(188..853)
                     /locus_tag="P5573_01925"
     CDS             complement(188..853)
                     /locus_tag="P5573_01925"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRT26854.1"
                     /translation="MRISEEIYVYEWTDYFDNNCNSFYIGGDAKVLVDPGLKRYVPNL
                     LESMEEDGVAREDIRYIINTHSHPDHYEGSVMFDGGGVGIGLHEEEIKFLDAGGKYLY
                     ELFGLTFPEVDVNLVLREGDITLGKEHFQVLLVPGHSPGSLALYWPSRKALFSGDVIF
                     AQNVGRTDFPGGNSVLLKRSILRLSQLDVDTLLPGHMGIIDGKERVKMNFDVVIEHIF
                     PYV"
     gene            complement(877..1680)
                     /locus_tag="P5573_01930"
     CDS             complement(877..1680)
                     /locus_tag="P5573_01930"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRT26855.1"
                     /translation="MASLLIGCTGPVVKKSENPRGVYHRVKKGETLWSISHAYHKDIL
                     DIVVANKIDNPDLIEIDSVIFIPGADHPEETAAAPAKAETTIKKEEAKPEKKLPPPPV
                     AREEKKAPPMKQAVPAVESPPKDRSQQRPPEKAESKKIADEVRFIWPVKGTVIARFGI
                     QPNGLKHNGIRIAAKEGTPVVASAAGEVMFSSSIKYYGETVIIKHDSSYSTVYTFLKN
                     RRVKVGDRVRRGECIAFVGRPENGDGKPCLNFEIRQNNKPRNPLMLLPQ"
     gene            complement(1752..2408)
                     /locus_tag="P5573_01935"
     CDS             complement(1752..2408)
                     /locus_tag="P5573_01935"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HRT26856.1"
                     /translation="MIDRFQKQRMRMVDTQLRSRGIKDTRVLKAMEKIPRHLFVDEAL
                     QDQAYNDCPLPIAENQTISQPYMVALMTEALGLKGKEKVLELGTGSGYQTAVLAELAD
                     RVFSVERIASLASRARKILDSLNYFNVAIRVGDGTYGWREEAPFDGIIVTAGAPEVPK
                     TLVEQLAVGGRLVIPVGGRYSQNLMRITRMSEDVDDIETENLGGCRFVDLIGEHGWKE
                     "
     gene            complement(2405..2821)
                     /locus_tag="P5573_01940"
     CDS             complement(2405..2821)
                     /locus_tag="P5573_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT26857.1"
                     /translation="MKTQLISKIATDNAEKTDLEILQERAEVLGRAGERLSAALESLK
                     NIGDTIEKKMASFYERTHNVQGPFPFKEVFDKHSQVEFLLEINSDIKRYNEAREYAKL
                     RFYYLIVTREAIGFRRHKSVEEQYPIPPKKKVLKRL"
     gene            2973..4553
                     /locus_tag="P5573_01945"
     CDS             2973..4553
                     /locus_tag="P5573_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRT26858.1"
                     /translation="MRTKSTRAMSAAVRQAYRRGEEEETLEPLVLFGGEGSPVGRIKK
                     GDSVIFYNIRGEREVELTRSLVEDGFEEFPVVEGMGLHFVTMVEYQRGLPVEVAFPPE
                     GVLMDTLSDVLSMLGLRQAKVTEAEKAAHLTYFLNGKKGEPLPGEERVIVPTRKDVVL
                     FDEAPEMSISRVTEAVVDKINDRACDVVFVNFPNVDVVGHIENERAVIRAVEAVDDHT
                     GRVVGEALRAGMQVIVTADHGTVEKWLYPDGVVDTGHTDSPVPFILAGADVGLTLRRE
                     GELADVAPTVLEIMGIPSPSAMTGRSLIEACGNMPPVRRRLLFLILDGWGINEDRAGN
                     LIARAGTPFMDGLWERFPHSKLKAAGEAVGLPEGTVGNSEVGHLHLGAGRRVYSDRSR
                     IDRAVRDGSFFRNTAFLNAMRAAKRNGRSLHLMGIVSFFSSHGSVDHLVALMEMAKRE
                     GLEDVFVHAMLGRRGEQPESGANYVEMIEKKAEEIGVGRVVSVIGRYWSMDREENWDR
                     VEKTYRMLIKGEGTPVYG"
     gene            4546..5112
                     /locus_tag="P5573_01950"
     CDS             4546..5112
                     /locus_tag="P5573_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HRT26859.1"
                     /translation="MDEKEGAMEIKVNGSRVVLVEGDITNEETDAIVNAANSRLGGGG
                     GVDGAIHRAGGPVIMAECRKIGGCPTGKAVLTTGGNLKARYVIHAVGPVYRDGKSGEA
                     ALLESAYRESLKLASAHGLKSVAFPSLSTGAYGYPLEEAARIALRTTIDYLKEHQGVE
                     LVRFVLFGRQAYDVFAGVLKELVAESKG"
     gene            5349..6506
                     /locus_tag="P5573_01955"
     CDS             5349..6506
                     /locus_tag="P5573_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRT26860.1"
                     /translation="MSTNRSFELGPIRPPSEARSLLIRVTRNCPWNQCTFCHTYRGEK
                     FEMRSVEEVKQDIRAMREIADEIIALSWKLGEGGKIGNATVDCVFNNELFYDECFRIV
                     AAWLYFGGESVFLQDSNSLVMKTDDLAEVIRFIREKFPDVRRITSYCRSLTAAKKTVE
                     ELKLLREAGLTRIHVGMESGYDPLLKFIRKGATAEQHIEGGRRIVAAGISLSEYVMPG
                     LGGVRWTKEHAVESARVLNQINPAFIRLRSLQVRKGTPLYDSMKNGLFEPLDEDGVVR
                     EIRTFLEHLEGIESRVVSDHILNLLEEVEGKLPEDRGKMVAVIDRYLGMPELDRLVFR
                     LGRRKGLYRRLDDLNADPLLYHRLREIVTRYLEEGPATMERDLDGVKQHFI"
     gene            6621..7127
                     /locus_tag="P5573_01960"
     CDS             6621..7127
                     /locus_tag="P5573_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HRT26861.1"
                     /translation="MFKVGDLAVYPAQGVGVIEAIEERDLMGNIQSFYIMKIFGSNMK
                     IMIPLDSVKSAGLRSVISAGEIPRIYEILKEKDVTIDKQTWNKRYRDYLEKIKTGSVY
                     EVARVLRDLCILKLDKDLSFGERKMMDTAKNLLIKEISVVSKSEETRVEEDINALFPM
                     RGMMKGIA"
     gene            7256..>7580
                     /locus_tag="P5573_01965"
     CDS             7256..>7580
                     /locus_tag="P5573_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HRT26862.1"
                     /translation="MTQLVIRFILIIACSLSGYFIAFNYTSFPVSLLGLLAGFLVAIF
                     VIQLEQAIRKVSLRVIFGGVIGTIIGLLIAFLFAYGLSFVSILERQDIAPWIYVLLTC
                     ILGYLG"
BASE COUNT         1663 a         1863 c         2236 g         1818 t
ORIGIN      
        1 cgggggcaga ccgagcactc ttcgagcagg gaaaaggccc tgtctatccg ttccgggaaa
       61 agtcccttct cgtacgtcgt caggtaagcc gctttgaagt cggcgcgctt cataggtttt
      121 gtgtatcgac cgaaaaaagg cggtgccgat tccatgcctt gcatcgttcc agtttgagaa
      181 gcacggctca gacgtaaggg aagatatgct cgatgacgac atcgaaattc atcttcaccc
      241 tttcctttcc gtcgattatc cccatgtggc ccggcagcag ggtgtctacg tccagttgag
      301 agagtctcaa gatgctcctt ttcaggagga cgctgttccc accggggaaa tccgtccttc
      361 ccacattctg ggcaaaaatc acgtctcccg agaagagggc cttcctcgaa ggccagtaaa
      421 gggcgagcga gccgggcgaa tgcccgggta cgaggaggac ctggaagtgt tctttgccga
      481 gcgtgatgtc tccctcgcgg aggacaaggt tgacgtctac ctccgggaag gtgagcccga
      541 agagctcata tagatacttt ccgccggcat cgagaaattt gatctcttct tcgtggaggc
      601 cgattcccac accgccgccg tcgaacataa cggaaccttc gtagtggtcg ggatgggaat
      661 gggtatttat gatgtaccgg atgtcttctc tcgcgacgcc gtcttcttcc atgctctcga
      721 gaaggttggg gacgtacctt ttcagccccg ggtcgacaag gaccttggcg tcaccgccga
      781 tgtaaaagct gttgcagttg ttgtcgaaat aatctgtcca ttcgtaaaca tagatttctt
      841 cgctgattct catgaaattt cctttcgggc gcttttttat tggggcagca acataagcgg
      901 gtttcgcggt ttgttgttct gtcgtatttc aaagttcagg cagggcttgc cgtcgccgtt
      961 ttcagggcgt ccgacgaagg cgatgcattc acccctcctg acgcggtctc ccaccttgac
     1021 cctcctgttc ttgaggaagg tgtagaccgt agaataactg gagtcatgct tgatgatgac
     1081 cgtttccccg tagtacttga tcgaagagga gaacatcacc tcgccggcgg cggaagcgac
     1141 gacgggggtg ccttcttttg cggctatccg tatcccgttg tgcttgagcc cgttgggttg
     1201 gatgccgaac cgggcgatga cagtgccctt gaccggccag ataaagcgga cctcgtcggc
     1261 gattttcttg gattccgctt tttccggcgg gcgttgttgc gacctgtcct taggcggaga
     1321 ctctaccgcg ggaacggcct gtttcattgg tggtgccttc ttttcttctc ttgccacagg
     1381 aggtgggggc aacttttttt ccggtttggc ctcttccttc tttatcgttg tctcggcctt
     1441 tgccggcgcc gccgccgtct cttccgggtg atccgccccg gggatgaaga tcacgctgtc
     1501 gatttcgatc aagtcggggt tgtctatctt attggcgacg acgatgtcca ggatgtcttt
     1561 gtggtaagcg tgagatatac tccagagggt ttcacccttt ttgaccctat ggtaaacccc
     1621 tcgggggttt tccgattttt ttacgacggg ccctgtgcaa ccgataagaa gagaagccat
     1681 cagtgccaga atgaacagtt ttccgtaagt gggcatggac aatcttcaac ccgtcgtcgc
     1741 caggtgcctc atcattcttt ccatccgtgc tcaccgatca ggtccacaaa ccggcatccc
     1801 ccgaggttct ccgtttcgat gtcgtcgaca tcttcagaca tacgggtgat tctcatcaga
     1861 ttctgagagt atcgaccgcc gacgggaatg acgagcctgc cgcccacggc gagttgctct
     1921 accaaggtct tcgggacctc cggggcccct gcagtcacga tgatgccgtc gaagggtgct
     1981 tcctcccgcc acccgtacgt tccatctccg acccggatgg cgacattgaa gtaattgagt
     2041 gagtcgagaa tctttctcgc cctcgacgcc agcgaggcga tccgctcgac ggaaaaaacg
     2101 cggtcggcca gttcagcaag gacggccgtc tggtaaccgg accccgtccc gagttcgagg
     2161 accttctcct tccccttgag tccaagggct tccgtcatca gggcgaccat gtaaggttgt
     2221 gatatagtct ggttctccgc gatggggagg ggacagtcgt tgtaggcctg atcctggagg
     2281 gcttcgtcga caaaaagatg ccgtgggatt ttttccatgg ccttcagtac cctcgtgtct
     2341 tttatgcccc tcgaccgcag ctgcgtgtcg accatcctca ttctttgttt ttggaatcgg
     2401 tctatcaaag tctcttcagc accttctttt tcggtggtat cgggtattgc tcctctacgg
     2461 atttgtgacg gcggaatccg atggcctctc tggtcacgat gaggtagtag aagcgcagtt
     2521 ttgcgtattc cctggcctca ttgtaccttt ttatatcact gtttatttcg agaagaaatt
     2581 caacctgtga atgcttgtcg aacacttcct tgaagggaaa aggaccctgg acattatggg
     2641 ttctttcata aaaagacgcc atttttttct cgattgtgtc gccgatattc tttaggcttt
     2701 ccagggctgc cgaaagacgt tccccggccc gtccgaggac ttcggcccgt tcctgcaaga
     2761 tctcgaggtc ggtcttttcg gcgttgtctg tggctatttt tgagatcaac tgcgttttca
     2821 tcccgatttt ttgtaacatt aagaaagcgg attttcaaca ggaaaggggt gactaaagtt
     2881 tcctttgcat ttgaaaaccc tttcatttag aaaaaacttt tgctcgtttt ccgacgatgt
     2941 ccggtgaatc tgagagagat ttggagaagg caatgaggac taaaagcacg cgtgccatgt
     3001 cggcagccgt caggcaggcc taccgcaggg gtgaagagga ggagaccttg gaacccctcg
     3061 tccttttcgg cggtgaaggt agtccggtcg gaaggatcaa gaagggcgac tccgtcattt
     3121 tttacaacat caggggcgag cgggaggtcg aattgacccg cagccttgtg gaagacgggt
     3181 ttgaggagtt ccccgtggtg gaagggatgg ggcttcactt tgtcaccatg gtcgaatatc
     3241 agcggggcct gccggtggaa gttgccttcc cgccggaggg ggttttgatg gataccttga
     3301 gcgatgtgct ttcaatgctg gggctccggc aggcgaaagt cacagaggcc gagaaggccg
     3361 ctcacctcac ttattttttg aacggcaaga aaggcgagcc tctgccgggt gaggagaggg
     3421 tcatcgtgcc cacgcgcaag gacgttgtcc ttttcgatga agcccccgaa atgtcgatct
     3481 cccgggtcac agaagcggtc gtggataaga taaacgaccg ggcctgtgac gtcgtcttcg
     3541 ttaattttcc gaacgtggat gtagttggtc acatcgagaa cgagcgggcg gtcatccggg
     3601 ccgtggaggc cgttgatgac cacaccgggc gggtcgtagg agaggcccta cgggcaggta
     3661 tgcaggtcat cgtgacggcg gatcacggga ccgtggaaaa atggctctac cccgacggcg
     3721 tcgtggacac gggccatacg gacagccccg tcccgttcat cttagcggga gcagacgtgg
     3781 gccttacctt aaggagggag ggcgagttgg cggatgtcgc cccgaccgtc ctcgaaatca
     3841 tgggtattcc cagcccctcg gctatgactg ggcgttcact tatcgaggcg tgcgggaaca
     3901 tgccgcctgt caggcggagg ttgttgttcc taatccttga cggttgggga ataaatgagg
     3961 accgggcggg aaatctgatc gcccgtgccg gaaccccgtt catggacggc ctctgggaac
     4021 gtttccccca ttcgaagctg aaggcggcgg gcgaagccgt gggcctgccg gaagggacgg
     4081 tgggcaattc cgaggtcggc catctccatc tcggggccgg taggcgggtt tattcggaca
     4141 ggtcaaggat cgaccgggcg gtcagggacg ggtctttttt caggaatacc gccttcctca
     4201 acgcaatgag ggcggcaaag agaaacggtc gctctctcca tctgatgggg atagtttcct
     4261 tcttcagctc tcacgggtcg gtcgaccatc tggtcgcgct catggagatg gcgaaacggg
     4321 aaggtctcga ggatgtcttc gttcatgcca tgctcggcag acggggtgaa caaccggaga
     4381 gcggggcgaa ttacgtcgag atgatcgaga agaaggccga ggaaatcggt gtcggccgtg
     4441 ttgtttcggt gatcggacgg tactggtcga tggaccggga ggaaaactgg gacagggtcg
     4501 aaaagaccta tcggatgctg ataaaagggg agggtacccc ggtgtatgga tgaaaaggag
     4561 ggagcgatgg aaatcaaggt aaacggttcg cgggtcgttc tggtagaggg ggacatcacg
     4621 aatgaagaga cagacgcgat cgtcaacgcc gccaactcgc ggcttggcgg cggcggcgga
     4681 gtagacggag ccatccacag ggcgggcggc ccggtcatca tggcggagtg caggaagatc
     4741 ggtggttgcc ctacggggaa ggccgtcctg accacgggcg gtaacctcaa ggcgcggtac
     4801 gtgatccatg ccgtcggccc tgtctatcgc gacgggaaga gcggcgaggc ggcgctcctt
     4861 gagagcgctt atcgggagag cctgaagctc gcctctgctc acggactcaa gagtgtcgcc
     4921 tttccgtcgc tcagcaccgg tgcctacggg tatccgctcg aagaggcagc aaggatagcg
     4981 ctgagaacga cgatcgatta cctcaaggaa catcagggtg tcgaacttgt ccgtttcgta
     5041 ctcttcggcc ggcaggccta cgacgtcttc gccggagttc ttaaggaact tgtcgccgaa
     5101 agtaaagggt gaaggtgtgg aacaatcggc atccggtgga atggaagcga ggcagtgggt
     5161 ggagagaagt agttgactgc ggagggcgtc tgacatagta ggcaggcttg acagaaggca
     5221 tagctgcccg taaaatttag agaaaagaca cataagtcga cagtggaggg gccttaacga
     5281 gttcgacgtt ttggcctttt tgcttttcgc tgtctgtttt ttacttcatc gcaggagaat
     5341 cgtaaattat gagtacgaac cgttcttttg aactcggccc catccgcccc ccgagcgagg
     5401 cgaggagtct gctcatccgc gtgacccgca actgcccctg gaaccagtgc accttttgcc
     5461 acacttacag gggagagaag ttcgagatgc gctccgtgga agaggtcaag caagatatcc
     5521 gggccatgag ggaaatcgcc gacgaaataa tcgccctttc ctggaaactc ggagaaggcg
     5581 gaaagatagg taatgcgacg gtagactgtg ttttcaacaa tgagctcttt tacgacgaat
     5641 gtttcaggat tgtcgcggcc tggctttatt tcggcggaga gtcggtcttt ttgcaggact
     5701 cgaactctct ggtcatgaag acggacgacc tcgccgaagt catccgcttt attcgcgaga
     5761 agtttccaga tgtcaggaga ataacgtcct actgtcgttc actgacggcg gcgaagaaga
     5821 ccgttgaaga gttgaaacta ctccgtgaag cggggttgac gagaatccat gtgggcatgg
     5881 aaagcgggta cgacccactt ctcaaattca tcaggaaggg ggccacggca gagcaacata
     5941 ttgaaggagg tcgcaggatc gtggcggccg gaatctcgct ttccgagtac gtcatgcctg
     6001 gcctcggcgg ggtacggtgg acgaaagagc atgccgtcga gtccgcccgg gtgttgaatc
     6061 agatcaaccc tgccttcatc aggttgcggt ccttgcaggt gaggaaagga actcctctgt
     6121 atgacagcat gaaaaatgga ttatttgaac ctctggatga ggatggggta gtccgggaaa
     6181 tcaggacatt cctcgagcac cttgaaggca tcgagagtag ggttgtgagc gatcatatac
     6241 tgaatctcct cgaggaagtg gagggaaagc tccctgaaga ccggggaaaa atggtcgccg
     6301 ttatcgaccg ttatctcggg atgccggaat tggatcggct ggtattcagg ctcggcaggc
     6361 gcaagggcct gtaccgaagg ctcgatgacc tgaatgccga ccctctcctc taccaccgtc
     6421 tccgtgaaat agtgacacgg tacctcgaag aaggtcccgc tacgatggaa agggacctcg
     6481 acggcgtcaa gcagcatttt atttgatgtg aaccgtttca gggcctataa ttcggctgaa
     6541 tcaaggattg aattgacctt gggcggggaa tatgttatgt aaaaaacctc agaaattccc
     6601 aaggaggtaa aaaggaaaag atgttcaagg taggggattt ggcggtatat cccgctcaag
     6661 gtgtgggtgt tatcgaggcg atagaggaac gcgacttgat gggaaatata cagtctttct
     6721 acatcatgaa gatcttcggc agcaacatga agatcatgat ccctcttgat agcgtaaaat
     6781 ccgcagggct gaggagcgta atctccgccg gcgaaattcc caggatttat gaaatcctca
     6841 aagaaaagga cgtcaccatc gataaacaga cctggaacaa acgctaccgc gactatttag
     6901 agaagataaa gacggggtcg gtttacgaag tggcaagggt gctgcgtgac ctatgcatcc
     6961 tgaaactcga caaggacctt tctttcggtg aaagaaagat gatggatacg gcgaaaaatc
     7021 tgttgatcaa ggaaatctct gtcgtcagca aatccgaaga aacgagagtc gaagaagaca
     7081 taaatgcctt gttcccgatg cgaggcatga tgaaaggcat tgcctaagaa ggtacttctt
     7141 ccagggtaca tggggagcca agaggcggcc atgtaccgtt ttttttctct catgggttct
     7201 ttgacatatt cataactacg tttaattaga aggataatct tagaaaggag gtagcatgac
     7261 acaactggtc atcagattca ttttgatcat agcctgttct ctgagcggtt attttatagc
     7321 ttttaattac acttcattcc cagtttcttt attaggttta ttggccggct ttttagtagc
     7381 aatatttgtg attcagctcg aacaggctat caggaaagta tccctcaggg tgatctttgg
     7441 aggcgtgatc ggaacgatta tcggactcct gatcgccttt ctttttgctt acgggcttag
     7501 tttcgtcagc atactggaaa ggcaggacat cgccccgtgg atatacgttc ttttgacctg
     7561 tatcttagga tatctgggtc
//
LOCUS       DAOTMX010000039         7490 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_8665_length_7490_cov_3.257431, whole genome shotgun sequence.
ACCESSION   DAOTMX010000039 DAOTMX010000000
VERSION     DAOTMX010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 7490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7490
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8665_length_7490_cov_3.257431"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2730)
                     /locus_tag="P5573_01970"
     CDS             complement(<1..2730)
                     /locus_tag="P5573_01970"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRT26863.1"
                     /translation="MISRFFIDRPVFASVISIVIVVAGLVALFNLPIAQFPEITPTQI
                     TVTALYPGANSEVVSQNVAAPIELQVNGADNMIYMMSTSSNTGNMSLSVYFDIGTDPE
                     MAQVDVQNRVNLALPQLPQAVTQQGVQVQKRSSTFMMIIGVYSPDERYDETYVANYTN
                     LYILDALKRIPGASQTSILGMPDYAMRIWLKPDRMAQLGITASDVADAIRKQNQQFAV
                     GQIGQSPTEGRVEQTFTVSTRGRFLEPEEFENIILRTSADGTAIVRLKDVGRAQLGSR
                     DYSVRCRINGKKTTLIGVYQQAGANALDVSNQVTKTLAEMKKSFPDGIDYTIAIDATK
                     FVHASIKEVVITFFEACLLVVLVVFLFLQTVRATVIPILAVPVSIIGTAVGMTLFGFS
                     INMLTLFGMVLAIGIVVDDAIVVIENVERNMTQLGLPPKEAAKKAMDEVTGPVIAIVL
                     VLCAVFIPVGFLGGMTGQLYKQFAITIAISVVISGIVALTLSPALAAILLSPHRGPKN
                     RFFRWFEEIFERITARYAEGVRWVMMNRLIALGCYIAVIGAVVFLFRFIPTSFVPSED
                     QCSILGVSFLPDTASLDRTGDVNRTAGQILSQFPAVKDVVEVDGFSLIDSQVQTNAGT
                     IFVSLKDYEARKEETMKVPALIEGAMAGFAGIREGLVIPLNPPSIPGLGTAGGFEFWI
                     QSRGEGSVAKLGAVTHDFVARANRQPELYGVQSSMRPFSQQMRVDVDREKAETLGVPV
                     EEIYDTLQILFGSVYVSQFNKYSRLWQVIVQAEPHYRSRPDDIGEVYVRSHKGQMIPL
                     KAVITMKYASGADLVTRFNNFPAARVMGNAAPGYSSGQAIEAMERVAREVLPADYSFA
                     WSGEAFEAKKAGVASSMVFAFGLLMVFLVLAAQYEKWSLPFGVLMAVPFALLGA"
     gene            complement(2744..4021)
                     /locus_tag="P5573_01975"
     CDS             complement(2744..4021)
                     /locus_tag="P5573_01975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRT26864.1"
                     /translation="MAENMTPLNRCNPRRLSIPWIPSLLITICLCLSFAALTGCKKEQ
                     KAEGLHGPVEVTAVKITPRDIPVEFEFIGQTESSHQVEIRSRVDGFLEKRLYEEGEIV
                     KAGQVMFQMDRRPFEASLQEAKGELAQQEARLANAKANLARIKPLAEKNAVSKKDLDD
                     AIGNERASRAAVISAKGKVRQAELNLSYTTIRSPVTGLSSRAKKQEGSYIAVGPESLL
                     TYVAKIDPIWVTFSVSENEFLKLKEGVKLGLVKLPPGDNYSVKVVLADGTVFPQKGVL
                     TFAEPTFNQETGTFLVRASLSNPRSISQLKPGQFVRVYLIGAVRPKAITVPRRAVQQG
                     AKGHFVWVVNKEGQAELRNVVVGEWYGDECFVTEGLKPDEMLVVDGIIKLAPGVPVKI
                     AEPTPAPPQTSEGAEPPGDRAEKQEAPSAPVKR"
     gene            complement(4171..4665)
                     /locus_tag="P5573_01980"
     CDS             complement(4171..4665)
                     /locus_tag="P5573_01980"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRT26865.1"
                     /translation="MRLFVVLLAGVLVFLPPKGNLSNLGLPPAEGATRDGVAPDFVLE
                     DLQGRQYRMSDYRGRPMFIVFGTTWCPMCRQEIPVYKEIYSRYGPKGLVVLYINVMES
                     QRKAAAYATKHSLPYPVLVDTQGKVTADYGVRGVPAKILVDRNGKIICWNCRSLDALL
                     SKQF"
     gene            complement(4688..5410)
                     /locus_tag="P5573_01985"
     CDS             complement(4688..5410)
                     /locus_tag="P5573_01985"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HRT26866.1"
                     /translation="MVSNVLEEVIGNLELYLQGSSALAYAAAYAGGLLVSFTPCIYPI
                     IPILAAYTGARGGASRARGFVLSVAYVLGIAVTYTALGAFAALTGRMFGEIQSNPLTF
                     FFVGNLCIFMGLSMLGVFSLSWQPGFAVRASMKERKGVVGSAVLGALSGIVMGPCTAP
                     VCAVLLGYVASKQNILFGISLFFVFSLGVGTLLVLVGAFTGLLVSLPRSGEWTVRVKK
                     IFGWVLIGAGEYFLITAGKLWF"
     gene            complement(5423..6271)
                     /locus_tag="P5573_01990"
     CDS             complement(5423..6271)
                     /locus_tag="P5573_01990"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRT26867.1"
                     /translation="MKIRYGKRHPERVPYVNIVLLLMTAATTLVAGALQQGVDPIAEP
                     SRLWEGLPFSVTLLFILGSHEFGHYFVSKRHGVEVTLPYFIPAPSFIGTFGAFIKMKS
                     PIMDRRVLLDIGAAGPIAGMIAAVPVVVAGLIVSEVRPGAAEGGIELGTSLLFAFLNW
                     VVHGYIPDEANIILHPVAFSGWIGLLVTSLNLLPVGQLDGGHVAYAILGRRQRKVAAA
                     VIALLFVLGVIGWSGWLIWVALLVLLGIYHPPVVYEWVTLDRKRRVVGWMTLALFVAT
                     FTPVPF"
     gene            complement(6370..6648)
                     /locus_tag="P5573_01995"
     CDS             complement(6370..6648)
                     /locus_tag="P5573_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRT26868.1"
                     /translation="MNKSELIEALSKKENLTEKKAMDVVNLLFNGFTEELKKGGRIEI
                     RGFGSFVVRSYDSYTGRNPKTGSNIDVAPKRLPFFKVGKELKEKINAS"
     gene            complement(6911..>7490)
                     /locus_tag="P5573_02000"
     CDS             complement(6911..>7490)
                     /locus_tag="P5573_02000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRT26869.1"
                     /translation="TKVYLVLNKPAGFVTTLSDPEGRPTVVDLLQGVTERVYPVGRLD
                     YDSEGLLLLTNDGEFALRVQHPRYGIPRTYLVKVRGVLKQSEVKKIQDGVMLEDGVFV
                     PLDVKVEKQNPLSTWLRMTIADGRNRVIRRLFDILGHPVARLVRVSIGGVELGGLKER
                     ECRHLKKREIEQLLRYVSRERKTGRKRRPVDG"
BASE COUNT         1607 a         2215 c         2114 g         1554 t
ORIGIN      
        1 cgccccaaga agggcaaaag gcaccgccat caagacgccg aaggggagcg accacttctc
       61 gtactgggcg gcaaggacca gaaagaccat gaggaggccg aaggcgaaaa ccatggacga
      121 ggcaacgcca gcctttttcg cctcgaaggc ctcaccgctc caggcgaagg agtagtccgc
      181 gggtagaacc tcgcgagcca cccgttccat tgcctcgatc gcctgccctg agctgtaacc
      241 cggggcggcg ttccccatca cccttgcggc ggggaagttg ttgaagcgcg tgaccagatc
      301 ggcccctgat gcgtatttca ttgtgatgac tgcctttaga gggatcatct ggcccttgtg
      361 cgatcgtacg tatacctcgc cgatatcatc cggtctcgaa cggtagtgcg gctcggcctg
      421 cacgatgacc tgccacaggc ggctgtactt gttgaattgg ctgacgtata cggacccaaa
      481 cagaatctgc agtgtgtcgt agatctcctc cacgggaacg ccgagcgtct ccgccttctc
      541 ccggtccacg tccacgcgca tctgctgcga gaaggggcgc atggacgact gcacgccgta
      601 cagctcgggc tgcctgttcg cgcgggcgac gaagtcgtga gtcacggccc ccagtttggc
      661 aacgctgcct tcaccacggc tctgtatcca gaattcaaat cctcccgctg ttcccaggcc
      721 ggggatggaa ggcgggttca gggggataac cagcccctcg cgtatcccgg cgaaccccgc
      781 catggcccct tcgatcaggg cgggcacttt catggtttct tccttacggg cctcgtaatc
      841 cttcaaagaa acgaagatgg tcccggcatt ggtctggact tgagagtcga taagactgaa
      901 gccgtccacc tcgacgacgt ccttcacggc gggaaactgg gagagaatct gcccggccgt
      961 ccggttcaca tccccggtcc ggtcgaggct tgccgtgtcg ggcaggaaac tcacgcccaa
     1021 gatgctgcac tggtcctccg agggtacgaa gctcgtagga atgaaccgga acaagaaaac
     1081 gaccgcccct atcacggcga tgtagcaccc cagggcgatg agcctgttca tcatcaccca
     1141 gcggacgcct tcggcgtagc gggccgttat cctttcgaag atttcttcga accacctgaa
     1201 aaaacggttc ttcgggcccc gatgagggga gagtaggatt gccgccagcg cgggcgacag
     1261 ggtcagtgcg acgattcccg agatcaccac ggagatggcg atggttatgg cgaactgctt
     1321 atagagctgt ccggtcatgc cgcccagaaa acctacaggg atgaagacgg cgcagaggac
     1381 gagaacgatg gcgatcacgg gccccgtgac ttcatccatc gccttcttcg ccgcctcctt
     1441 cggcgggagg ccgagctggg tcatgttgcg ctcaacgttt tcaatcacca cgatggcgtc
     1501 gtccacgacg atcccgatcg ccagaaccat cccgaacagg gtcaacatgt ttatggagaa
     1561 cccgaacagg gtcatcccga cggcggtacc gatgattgaa acgggaacgg ccaggatcgg
     1621 gatcacggtc gcccggacgg tctgaaggaa gaggaagacc acgagaacga cgagtagaca
     1681 ggcctcgaag aaggtaatca ccacctcctt gatggaggcg tggacaaact tcgtggcgtc
     1741 tatcgcgatc gtataatcga tcccgtcggg aaaacttttc ttcatctcgg ccaaggtctt
     1801 cgtgacttgg ttggagacgt ccagggcatt cgcgccggcc tgctggtaaa cgcctatgag
     1861 tgtcgtcttt ttcccgttta tccggcagcg cacggagtaa tcccgcgaac cgagctgggc
     1921 gcgacccaca tccttgagcc ttacgatggc cgtgccgtcg gcgctcgtcc tcaggatgat
     1981 attctcgaac tcttcgggct ccaggaaccg tcctctggtc gaaaccgtga aggtctgttc
     2041 tacccggcct tcagtggggg actgtccgat ctgtcccacg gcaaactgct ggttctgctt
     2101 cctgattgcg tcggccacgt cgcttgccgt gatgccgagt tgggccatgc ggtccggttt
     2161 cagccagatg cgcatcgcgt agtcaggcat gccgaggata gaagtctggc tcgccccggg
     2221 gatcctcttc agggcgtcca ggatgtatag gttcgtgtag ttggccacat aggtctcgtc
     2281 gtaacgttca tcgggggagt agacgccgat gatcatcatg aaggtggatg atcttttctg
     2341 cacctgtacg ccctgctggg tgacggcctg cgggagctgc ggcagggcga gattcacacg
     2401 gttctggacg tccacctgcg ccatctcggg atctgtgccg atatcgaaat acacgctgag
     2461 tgacatgttg ccggtgttgg aactcgtgga catcatgtag atcatgttgt cggcgccgtt
     2521 gacctgcagt tctatgggag cggcgacatt ctgcgagacc acctcggagt tggctcccgg
     2581 gtacagggct gtaaccgtga tctgggtagg cgtgatttcc ggaaactgcg caataggcag
     2641 gttgaagagg gcgacgagac cggcgacgac gatgacgatg gaaatgacgg aggcgaagac
     2701 cggccggtcg atgaagaagc gggagatcat gcgggagatc ctgtcatctc ttcaccggtg
     2761 cggatggggc ttcctgcttc tcggcccggt cccccggcgg ttctgcgccc tcagaggtct
     2821 gcggcggcgc aggggtcggt tcggcgatct tgacggggac gcccggtgca agttttatga
     2881 tgccgtcgac gaccagcatt tcatcgggtt tcaggccttc ggtgacgaaa cactcgtcgc
     2941 cgtaccattc cccgacgaca acgtttcgaa gttccgcctg cccctccttg ttgacgaccc
     3001 agacgaaatg gcccttcgct ccctgttgga cggcacggcg cggcaccgtg atagccttgg
     3061 gacggacggc gccgatgaga tacacccgca cgaactgccc gggcttcaac tgcgatatac
     3121 tccggggatt ggaaagggag gctcgcacga gaaaagtgcc cgtttcctga ttgaaagtcg
     3181 gttcggcgaa ggtgaggacg cccttttgcg ggaagacggt cccgtcggca agaaccacct
     3241 tcaccgagta gttatctccg gggggcaact tgaccaagcc caatttgacc ccctccttca
     3301 gcttcaggaa ctcgttttcc gagacactga aggtgaccca gatcggatcg atcttggcca
     3361 cgtaggtcaa cagactttcc ggccccacgg ctatatagct gccttcctgt ttcttcgccc
     3421 ggctcgatag gccggtcaca ggggaacgga tcgttgtgta gctcaggttc agctccgcct
     3481 ggcgcacctt ccctttggcg gatatgaccg ccgcccggga ggcccgttca tttccgatcg
     3541 cgtcgtcgag gtctttcttg cttacggcat ttttttccgc caggggcttg atgcgggcga
     3601 ggttagcctt ggcgttggca agccgggctt cctgctgggc caactcaccc ttagcctctt
     3661 gcagggacgc ctcgaaagga cggcggtcca tctggaacat gacctgtccc gccttgacga
     3721 tctcgccctc ttcgtacaat cgcttttcca ggaaaccatc cacacgggag cggatctcca
     3781 cctgatggga actttccgtc tggcctatga attcaaattc taccggaatg tcgcggggcg
     3841 tgatcttgac agccgtcact tcaacggggc cgtggaggcc ttcggccttc tgctctttct
     3901 tgcaccccgt cagagccgcg aacgaaagac agagacatat ggtaatgagg agagaaggta
     3961 tccatggaat cgagaggcgt cgaggattgc atctattcaa cggggtcata ttctctgcca
     4021 ttccgggtta ggtttcttgt ttgtccgtag tagaaagaga ttccggctaa atcgtttcct
     4081 tcctattagg aaataagcat aggagtgtca agagataaaa cagattgcac cgagttatcc
     4141 aaagtaaaag ggtgatcggt ttaatagaaa tcaaaactgt ttcgacagca aggcgtccaa
     4201 agagcggcag ttccagcaga tgatcttacc attcctgtcc acgaggatct tcgctggcac
     4261 tccgcgcacg ccgtaatcgg ccgtgacttt tccctgtgtg tcgaccagta cgggataggg
     4321 cagtgaatgc ttcgttgcgt aagcggcggc ctttctttgc gactccatga cgttgatgta
     4381 gaggacaacc aaccccttcg ggccgtaccg ggagtagatt tccttgtaaa caggtatctc
     4441 ctggcggcac atagggcacc atgtcgtacc gaagacgatg aacatcggcc gccccctgta
     4501 gtcgctcatc cggtattgcc ggccttgcag gtcttccagg acaaaatcag gggcaacgcc
     4561 gtcacgcgtt gccccttccg ccggtggaag cccaaggttc gacaggttgc ccttcggggg
     4621 aagaaagacc aggacgcccg ccaaaagtac cacgaacaat ctcaccaatc cgttcaggac
     4681 atccctctca aaaccagagt ttcccggctg tgatcagaaa gtattcccct gccccgataa
     4741 ggacccagcc gaagattttc ttgaccctga ccgtccattc cccgctcctc ggaaggctca
     4801 caagaagtcc cgtgaaagca ccgaccagga cgaggagcgt gccgacgccg agggaaaaga
     4861 cgaagaagag acttatgccg aagagtatgt tttgtttcga ggccacgtac ccgaggagaa
     4921 ccgcacaaac cggcgccgtg cagggaccca tcactatgcc cgaaagggcc cccaggacgg
     4981 cgctgccgac aacccccttc cgttctttca tcgatgccct cacggcaaaa ccaggctgcc
     5041 aggacaggga aaagaccccc agcatagaaa ggcccatgaa aatacagagg ttgccgacga
     5101 aaaaaaaggt caacgggttg gactggatct ccccgaacat acggccggtc agcgccgcaa
     5161 aggccccgag agcggtatac gtgacggcaa tccccaagac ataggcaacc gagagcacaa
     5221 agccccttgc cctcgaggcg ccgccccgcg cgcccgtgta agcagcaagg atagggatta
     5281 tggggtagat gcagggagtg aagctcacaa gcagaccgcc cgcgtaggcg gccgcgtagg
     5341 cgagggcaga agacccctga aggtacaact caagattgcc gataacctct tcgagcacgt
     5401 tcgacaccat ggaccgatcg catcaaaacg gtacgggagt aaaggttgcg acgaagagag
     5461 caagggtcat ccagcctaca acccttctct tcctgtcgag cgtcacccac tcgtaaacca
     5521 ccggcgggtg gtaaatcccc aggaggacga gaagagcgac ccagatcaac catccgctcc
     5581 atccgatgac acccagcaca aacaatagtg cgatgacggc ggcggcaact ttgcgctgcc
     5641 tgcggccgag tattgcgtag gcaacgtgtc cgccgtcgag ctggccgacg ggcaggaggt
     5701 tgagggaggt taccagcagg cctatccacc ccgaaaaggc cacgggatgg aggatgatgt
     5761 tcgcctcgtc ggggatatag ccgtgaacaa cccagttcag gaacgcaaaa agcaatgatg
     5821 tgccgagctc tatcccgcct tccgccgccc ccggccttac ctcggaaacg atcaatccgg
     5881 caacgaccac cggtacggcc gcgatcattc ctgctatcgg gcctgcggcc ccgatgtcga
     5941 gaaggaccct cctgtccatg atcggcgact tcatcttgat gaaggccccg aaggttccga
     6001 tgaaggaagg cgcggggatg aaataaggga gcgtgacctc gaccccatgc cttttggaaa
     6061 cgaagtaatg cccgaattca tgggacccga ggataaaaag cagggtgacc gaaaagggaa
     6121 gtccctccca cagccggctg ggttccgcga tggggtcgac tccctgttgc aaggcgccgg
     6181 cgacgagggt cgtcgccgcg gtcatgagca agagaacgat gtttacgtat gggacccgct
     6241 cagggtgtct tttgccgtac ctgatcttca tgattgatga cgcaaagaaa aagggaccat
     6301 tacgtcatga tcccttctct gatgtgtata gacggttcaa aaaatccggc tcgaagcgaa
     6361 aagccttgtt caagaggcgt tgatcttttc tttgagctct tttcctacct tgaagaacgg
     6421 cagcctcttc ggtgccacat cgatattgct gcccgtcttc ggattcctgc ctgtatagga
     6481 atcataagac cttacgacaa aactcccgaa ccccctgatc tcgatacggc cgcccttttt
     6541 cagctcttcg gtaaaaccgt tgaacaacag attgacgacg tccatggctt tcttttccgt
     6601 caggttctct ttcttgctca gggcttctat caattccgac ttgttcatga gatctctcct
     6661 ttgcgatcat tcataatagt ttcccgacgc acaccacgac tatcgtcgca ttcattaatg
     6721 acaatttgac cgtatatcca cgatgttact gatgacagcc gtcgtcacta ttattcactt
     6781 tctctcccaa agtcaagcta attttgactc tttttcgctc gatgtctaat aaaatcaacg
     6841 gataccgtgt ctccctcgcg gccgggggcg gattttcgct gatttttcgc ctcccgaccc
     6901 atcggcgtcc tcaaccatca acaggtcggc gtttcctgcc cgtttttctc tcccgggaaa
     6961 cataacggag caactgctcg atttcccgct tcttcagatg cctgcattcc ctctccttca
     7021 ggcctccgag ctcgacgccg ccgatagaga cgcggacgag gcgcgccacc ggatgtccca
     7081 ggatgtcgaa aagcctccgt atcacccggt tccggccgtc cgcgatggtc atgcgcagcc
     7141 aggtgctcag ggggttctgt ttttcgacct tcacgtcgag ggggacaaac accccgtcct
     7201 ccagcatcac gccgtcctgt atcttcttga cttcactctg cttcaagacc cctctaacct
     7261 ttaccaggta agtcctgggt atcccgtatc gcgggtgttg caccctaagg gcaaattcgc
     7321 cgtcgttcgt caggagcaga agtccctccg aatcgtaatc cagcctaccg acggggtaaa
     7381 cgcgctccgt gacgccctgg agaaggtcca ccactgtggg ccgcccctcc gggtcgctca
     7441 acgtcgtcac aaaacccgcc ggtttgttga gcacgaggta gaccttagta
//
LOCUS       DAOTMX010000040         7400 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_8786_length_7400_cov_3.592784, whole genome shotgun sequence.
ACCESSION   DAOTMX010000040 DAOTMX010000000
VERSION     DAOTMX010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 7400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7400
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8786_length_7400_cov_3.592784"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="P5573_02005"
     CDS             <1..518
                     /locus_tag="P5573_02005"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HRT26870.1"
                     /translation="LDFEVIPAGIDESFRDGETPYEHVLRLSREKARAVAGAHPEAWV
                     LGADTIVLVNGEVLGKPGTKREAKAMLGKLSGREHRVVTGFTLLKDGGATAATEAVES
                     VVVFKKLTPDEIEWYAGTDEPYDKAGGYAAQGKGASLIKEIRGSYTNVVGLPLCEVME
                     ALKTVGAAKVC"
     gene            521..1219
                     /locus_tag="P5573_02010"
     CDS             521..1219
                     /locus_tag="P5573_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HRT26871.1"
                     /translation="MSSVAENVRRVRDVIEDAAVKAGRNPSKVRLMAVTKTVEDDRIL
                     EAIEAGVDIIGENYVQEGKRKVEKLGRRGVEWHLIGYLQSNKAKYALKIFDMIHSVDR
                     LSLAQEIDRRAGAAGSVAKILVEVNLSGEATKGGVKKDEALALVRDIAKLENLSVRGL
                     MTMPPWFDDPEEARPYFAALRELKDRVIDEKIGNVLMEELSMGMTGDYRVAVEEGSTI
                     VRVGRGIFGERPSR"
     gene            complement(1260..2219)
                     /locus_tag="P5573_02015"
     CDS             complement(1260..2219)
                     /locus_tag="P5573_02015"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRT26872.1"
                     /translation="MKKIYVKDIKAGDKVTDTFLAVEKNLAFSQKGSPYLNVRLRDRT
                     GDIEGRVWERATDINKLFKKGDVVQVNSRAASYKSALQLSILDIRKTDGEEVDLRDYA
                     PVSRFAVEDMLGELFHYIEGVKNPHLRGLLDAFFQDEHVVERFKLAPAAKGFHHVYLG
                     GLLEHTVSVLKLLDLVSTHYDNIDRDLLIAGGILHDIGKIAELSSGPVFEYTDTGRLI
                     GHIVLGVEMLDAKVATLDNFPEQLAMELRHIILSHHGILEYGSPKRPKTLEALVIHYI
                     DDLDAKVNAFQGFIDNADEDSDWTPFHRLFERFIYKGKQQNEG"
     gene            complement(2241..2999)
                     /gene="fdhD"
                     /locus_tag="P5573_02020"
     CDS             complement(2241..2999)
                     /gene="fdhD"
                     /locus_tag="P5573_02020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HRT26873.1"
                     /translation="MRAAMEKGAVKFFNTGELENLPADIVCEVPLSLYLNGGRISTIA
                     CTGLHLRELAVGFLRSEGLLVSRDEIKDILVVEGPEPAVYVYTFERMPPGGETNYIGS
                     GGARGRNGTGTGLKPFEGVSLRLSPQNILDLMEELQQASVIHEATRGTHCSGLAASLG
                     MILTREDIGRHNTIDMIGGHMLLEGMEGSDMVIVTTGRVSSEIVTKVRAMGVPVIVSH
                     SAPTTRAIVICRELAMTLVGCVRGGTFRVYTGFE"
     gene            complement(3007..4926)
                     /gene="selB"
                     /locus_tag="P5573_02025"
     CDS             complement(3007..4926)
                     /gene="selB"
                     /locus_tag="P5573_02025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HRT26874.1"
                     /translation="MKNIVLGTAGHVDHGKTALIKALTGVDTDRLKEEKERGITIELG
                     FAALTLPNGRRIGIVDVPGHERFVKNMVAGAGGIDMVLLVIAADEGVMPQTREHLDIC
                     TLLGVKEGLVALTKVDLVDDEWLSMVEEDVREFLGGTFLHDAPIVPVSTVANKGLAEL
                     LSAIEERASRVEESADTDIFRLPIDRVFTMKGFGTVVTGTIMSGKVRVGEAVEVYPGR
                     KKARVRGLQVHNAAVEEATSGQRTAVNIQGIDRDAIERGDVLGQPDTLEPSTRFDVFF
                     EYLPSAPRRLKNRTLVRFHAGTSETIARVILLDRDDVGPGEKCFAQVVPESPVIAMAR
                     DRFVARSYSPVRTVGGGEILDPSPRKHKRGQPGVLEEMRTLLEGSEYERTANIIRRAG
                     LQGITVPSLSVRTGISPAGIEKILREMYSRKEAVLWDKETQRIVSFPLYSKLQEKVVA
                     ETGSYHKRSPLRAGVSKEELRMTVGGFIDPKLFNKALQDLEKTGKITVEKESVRLPTH
                     RVSLQGDLAEARDVISEICLQAGLAPPTGKEILERMAGRFPDADKVLEVMLDEGILVK
                     VGDDLYFHRDTVGKLREDYRHLLLKEGKSTPSSFKDLTGLSRKFIIPLMEYFDATKLT
                     IRVGDHRVLRDRQGK"
     gene            5145..5600
                     /gene="ruvX"
                     /locus_tag="P5573_02030"
     CDS             5145..5600
                     /gene="ruvX"
                     /locus_tag="P5573_02030"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HRT26875.1"
                     /translation="MRILALDYGERRIGVAVSDELGITAQGLTTLIRKNLQQDMGEIT
                     KVVEAYNVDRVVVGYPLRLDGTRGIQCEKVDRFIGVLEKTLAVPVERWDEGLSTWEAE
                     EVLTEALVKGRKRKAVVDKIAACVILRDYLSHLRRQNTMNEEVEDPSWK"
     gene            5591..6607
                     /gene="mltG"
                     /locus_tag="P5573_02035"
     CDS             5591..6607
                     /gene="mltG"
                     /locus_tag="P5573_02035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HRT26876.1"
                     /translation="MEMIKAFNERKFPIMMSFVAAAMFLVAAFLVCIRSPIDHNDVRV
                     TVEIPKGASFTAAASILSDAGLIKHRTLFHLLARLKNAQYRVRAGEYELSTAMSPANI
                     LDKLIMGEVVEYTVSIPEGYDLRKIADRLAEDKLVKKDVFLRLAKDPEFLASLGITAG
                     SLEGYLFPDTYVFNRKMGEEEIIKMMVRRFEEKVTPEMKKRAMSLGLTPNQLLTLASL
                     IEKEAKLKSERALISAVFHNRLQKGMKLQCDPTAVYGLADFEGPIKKSHLRRKSPYNT
                     YVINGLPPGPIASPGLESVMAALYPAPVDYIYFVSRNDGSHQFSSDLRSHNRAVSKYI
                     TRKD"
     gene            6696..7142
                     /gene="mraZ"
                     /locus_tag="P5573_02040"
     CDS             6696..7142
                     /gene="mraZ"
                     /locus_tag="P5573_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HRT26877.1"
                     /translation="MEVFRGHYHHTIDSKGRIIFPAKLREVFAEKYDNRLVITNWDGY
                     LMVFPYDEWRILEEKVIHQSLLRKEVRAFQRFFMSGAVDCTFDSQGRILIPPPLREFA
                     KLEKDVVLAGMGKVIEIWDKERFEAATDPSKIDMDKFGEYMADLGI"
     gene            7176..>7400
                     /gene="mraW"
                     /locus_tag="P5573_02045"
     CDS             7176..>7400
                     /gene="mraW"
                     /locus_tag="P5573_02045"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HRT26878.1"
                     /translation="MFSSSYHQPVMVAGVLRFLACRPGGVYVDGTVGGGGHAAAILEA
                     TAPDGRLIGIDLDDEALAEAHRVLRRGGRFF"
BASE COUNT         1598 a         2052 c         2120 g         1630 t
ORIGIN      
        1 ggctggactt tgaagtgatc cccgccggca tcgacgagtc cttccgggac ggagaaacgc
       61 cctatgaaca cgtcttgcgg ctgtcaaggg aaaaggcccg ggcagtcgcg ggggcgcatc
      121 ccgaggcatg ggtcctagga gccgacacca tcgtcctcgt aaacggggaa gtcttgggaa
      181 agccggggac gaagcgggaa gccaaggcca tgctcggcaa gttaagcggc cgggaacacc
      241 gggtcgtcac ggggttcacg ttgctgaaag atggcggcgc gacagccgcc acggaggccg
      301 ttgaatccgt cgtcgtcttc aagaaactga cgcccgacga gatcgagtgg tacgccggca
      361 cggacgagcc ctacgacaag gccggcggtt atgccgccca ggggaaggga gcctctctca
      421 tcaaggagat cagggggtca tacacaaacg tcgtcggtct cccgctctgt gaagtcatgg
      481 aggcgctgaa gacagtggga gcggcgaagg tctgttagac atgtcttcgg tcgcagaaaa
      541 cgtacgcagg gttcgtgacg tcatcgagga tgcagccgta aaggccggac ggaatccctc
      601 caaggtcaga ttgatggccg ttaccaagac cgtggaggac gaccgcatac tggaggctat
      661 cgaagcgggc gtagacatca tcggcgaaaa ttacgtccag gaaggaaaga ggaaggtaga
      721 gaagctcggt aggaggggag tcgaatggca tctcatcggt tacctccagt ccaataaggc
      781 taagtacgcc ctaaaaatct tcgacatgat tcattcggtc gaccggttaa gcctggccca
      841 ggaaatagac aggcgggctg gggcggccgg ttcggtcgcg aagatcctgg tcgaggtgaa
      901 tctgagcggg gaggcgacaa agggcggtgt aaaaaaagac gaggccctcg ccctcgtcag
      961 ggatatagcg aaacttgaga acctctcagt gcgcggtttg atgaccatgc cgccctggtt
     1021 tgatgacccg gaagaggcga ggccttactt cgccgcccta cgggagctca aggatagggt
     1081 tatcgacgaa aaaatcggga acgtcctgat ggaagagctt tccatgggga tgacgggcga
     1141 ttatcgggtc gccgtagagg aaggctctac catcgtcagg gtcggacggg gcatcttcgg
     1201 agaacgccct tcccggtaac gcagtcgtca attccggcct gcatttcttc tctatcattc
     1261 tacccttcat tctgttgttt gcccttgtat ataaaacgct cgaagagacg gtggaacggc
     1321 gtccagtcgg agtcctcgtc ggcattgtcg atgaatccct gaaaggcgtt gaccttggcg
     1381 tcgaggtcat cgatgtaatg gatgacaagg gcctcaagag tcttcggcct cttcggtgac
     1441 ccgtactcca ggatgccgtg gtgactcagg attatgtgcc gcagttccat ggcgagctgc
     1501 tcggggaagt tgtcgagggt tgcgaccttg gcgtccaaca tctccacgcc gaggacgata
     1561 tggccgatca ggcggcctgt gtcggtgtac tcaaagaccg gacccgacga gagttcggcg
     1621 atcttgccga tgtcatgcag gataccgccg gcgatgagga ggtcgcggtc gatgttgtcg
     1681 tagtgggtcg acaccaggtc gagcagtttc agcacggaga ccgtgtgttc gagaagaccg
     1741 ccgagataga catgatgaaa cccctttgca gcgggggcaa gcttgaacct ctcgaccaca
     1801 tgctcgtcct gaaaaaaagc gtcgaggagt cccctgaggt ggggattctt gaccccttcg
     1861 atgtagtgga aaagttcacc gagcatgtcc tcgacggcga agcgcgagac gggggcataa
     1921 tcccgcaggt caacttcctc cccgtccgtc ttacggatgt ccaggatgga aagctgcagg
     1981 gcgctcttat agctagccgc cctggaattg acctgaacga cgtcgccctt cttgaacaac
     2041 ttgttaatgt cggtagccct ctcccagacc cgcccttcga tgtctcctgt cctgtccctc
     2101 agacgtacat tgagataagg agatcctttc tgggaaaagg ccaggttctt ttcgacggcg
     2161 aggaaggtgt cggtgacctt gtccccggcc tttatgtctt tgacgtatat ttttttcatt
     2221 ccgggctcct tgaaacctcc ttattcaaac cccgtataaa ccctgaacgt tccgcccctc
     2281 acgcagccga cgagggtcat ggcgagttct ctgcaaatga cgatcgccct tgttgtgggc
     2341 gccgaatgtg agacgatgac cgggacaccc atcgccctca ccttggttac gatctcggag
     2401 gaaacgcggc ccgttgtgac gatgaccatg tctgagcctt ccatgccttc caggagcata
     2461 tggccgccta tcatatcgat ggtgttgtgc cggccgatat cctctctcgt gagaatcata
     2521 cccagcgaag cggccaaccc agagcagtgc gtaccccgcg tcgcctcgtg gatgaccgag
     2581 gcctgttgga gctcttccat gaggtcgagg atgttttggg ggctgagtct caaggaaacc
     2641 ccttcgaagg gcttgaggcc ggttccggtc ccgttccttc ccctggcgcc gcccgagccg
     2701 atgtaattcg tttctcctcc tggtggcatt ctttcaaagg tgtatacgta aacagcgggc
     2761 tcgggccctt caacgactag gatatccttt atctcgtccc gtgagacgag gagaccctcg
     2821 gaacggagga aacccacggc caactcgcgc aggtgcagac ccgtgcaggc gatagtggag
     2881 attctccctc cgttgagata caggctgagc ggcacctcac acacgatatc tgcgggaagg
     2941 ttctccagtt ccccggtgtt gaaaaatttg accgccccct tttccattgc cgcccgcata
     3001 ttcgcctcac ttgccttgcc gatcccgcag gacccggtga tctccgaccc tgatggtgag
     3061 tttcgttgcg tcgaaatact ccatcagtgg gatgatgaac tttcgcgaca gacccgtcag
     3121 gtctttgaag gacgagggcg tcgatttgcc ttccttgagt aggagatgcc tgtagtcctc
     3181 ccttagtttc ccgacggtgt cgcggtgaaa gtagaggtca tcccctacct tgaccagtat
     3241 cccttcatcg agcatgactt ccagaacctt gtctgcatcg gggaatcggc cggccattct
     3301 ttccaggatc tcctttcccg tcgggggcgc tagtccggcc tgaaggcata tttcgctgat
     3361 cacatcccgg gcctctgcaa ggtctccctg gaggctgacc cgatgggtgg gaagacggac
     3421 gctctctttc tcgacagtta tctttcctgt tttttccagg tcttggaggg ccttgttgaa
     3481 cagcttaggg tcgataaaac caccgacggt catccgtagc tcttcctttg aaacccccgc
     3541 ccttagggga gaccgtttat ggtagcttcc ggtttccgcg acaacctttt cctgaagctt
     3601 cgagtacaag gggaatgaaa cgatgcgttg cgtctcctta tcccagagaa cggcttcctt
     3661 ccttgaatac atctcccgca ggattttttc aatacctgcc gggctgatac ccgttctgac
     3721 cgaaagggag gggacggtta tgccttgcag gccggcccgc cggatgatgt tcgccgttct
     3781 ctcgtattca cttccctcga gaagcgttct catctcctcc agaacacctg gttggcctcg
     3841 tttgtgcttg cggggcgagg ggtcgaggat ttctcctccg ccgacagtcc ttacggggga
     3901 ataactcctc gcaacaaatc ggtctctcgc catggcaatg acgggagatt caggcacgac
     3961 ctgggcgaaa cacttctctc cgggcccgac gtcgtcccgg tcgaggagta tgacacgggc
     4021 gatcgtctcg ctcgtccccg cgtgaaacct cacaagggtc ctgtttttca ggcgcctcgg
     4081 cgccgaggga aggtattcga agaagacgtc gaaccgggtg gagggttcaa gggtgtcggg
     4141 ctggcctagg acatcccccc tctcgatcgc gtctctgtcg attccctgta tgtttacagc
     4201 tgtacgctgt cccgatgtcg cttcctccac tgccgcattg tggacctgga gaccccggac
     4261 ccgcgccttt tttctcccgg gatagacctc tactgcctct ccgaccctca ctttcccgga
     4321 catgatcgtc cccgtgacca cagttccgaa gcccttcatc gtgaagaccc tgtcgatggg
     4381 gagtctaaaa atatccgtgt cggcggattc ttcgacccgg gaggcccttt cctctatggc
     4441 ggacaaaagt tcggcgagtc ccttgtttgc cacagtggac accgggacga tgggggcatc
     4501 gtgaagaaaa gtacccccca agaattcacg gacgtcctcc tcgaccatgg atagccattc
     4561 gtcgtcgact aggtcgacct tggtcagggc aacgaggccc tctttcacac ccaagagagt
     4621 gcagatatcc aggtgctccc tggtctgggg catgacgcct tcgtcggctg ctatgacgag
     4681 aaggaccatg tcgatacccc cggcgccagc caccatgttc ttgacgaacc gttcgtggcc
     4741 cggtacgtcc actatgccga tgcgccgtcc attcggcagc gtgagggcgg cgaaaccgag
     4801 ttcgatcgtt atcccccgct ccttctcctc tttcagcctg tccgtgtcga ccccggtcaa
     4861 ggccttgata agggcggtct ttccgtgatc cacgtgaccg gcggtgccga gaacgatatt
     4921 tttcacctcc tgcttcctcg acctgcttcc ttgctcgtaa ccctgaattc ggtcccaaaa
     4981 acgggaaaag ccgccttttt ggttccttta gcggacctct tttgtgtcac ggtgaggata
     5041 gcaaaatcat taaattcaca caagaaaaaa cagacaacgg gctctctgtg gcgaaaaatc
     5101 gttgtcaagg gcgtttcata cgtgttactc tttaacatca tcttatgaga atactggccc
     5161 ttgactacgg cgaaaggagg atcggggttg cggtctccga cgaactgggg attacggctc
     5221 aaggtctcac tacgctgatc cgcaagaacc tgcagcagga catgggggag ataacgaaag
     5281 tagtcgaggc ctacaatgtg gaccgggtcg tcgtcggcta cccgctacgg ctcgacggga
     5341 cgaggggaat ccagtgtgag aaagtggaca ggtttatagg tgtcctcgag aagacccttg
     5401 ccgttccggt ggagcggtgg gatgagggac tttcgacatg ggaagcggaa gaggtactga
     5461 cagaagccct cgtgaaggga agaaagagga aagcggtggt ggataaaatc gccgcctgcg
     5521 tcattctgcg ggattatctg agtcacctaa gaaggcaaaa tacgatgaac gaggaggttg
     5581 aagacccgtc atggaaatga tcaaggcgtt caatgaaagg aaatttccga ttatgatgag
     5641 ctttgtcgct gcggcgatgt tcctggtcgc cgctttcctt gtctgcatac gcagtccgat
     5701 agaccataat gatgtccggg ttaccgtcga aatcccgaaa ggagcaagtt tcacagcagc
     5761 agcctccata ctcagcgacg ccggcctcat aaagcaccga acccttttcc acctcttggc
     5821 caggctgaag aacgcccagt atcgtgttcg tgccggggag tatgaactca gcacggccat
     5881 gtcccccgcg aacatcctgg acaagctcat tatgggcgag gtcgtcgaat acaccgtatc
     5941 cattcctgag ggttacgacc tccgaaagat cgcggaccgc ctggctgaag acaagcttgt
     6001 gaagaaggat gtcttcctga gactcgccaa ggacccggaa ttccttgcct ctctcgggat
     6061 aaccgcaggg agccttgaag gttacctttt tcccgatact tacgtattta accggaagat
     6121 gggcgaagag gagatcatca agatgatggt acgccgtttc gaagagaagg taaccccaga
     6181 aatgaaaaag cgggcgatgt ccctcggcct gacaccgaac cagttgctga cattggcatc
     6241 actgatcgaa aaagaggcca agctgaagag cgaaagggca ttgatatcgg ccgtttttca
     6301 caatcgcctg caaaagggca tgaaactgca gtgcgaccca acggccgttt acgggctcgc
     6361 tgactttgag ggcccgatca agaaaagcca cttgaggcga aagtcaccct acaatactta
     6421 tgttatcaat ggattgccgc cgggacccat cgccagcccc gggctcgagt ccgtgatggc
     6481 agccctctac cctgcgcccg tcgattacat ctatttcgtt tcgcgtaacg acgggtctca
     6541 ccaattttcc tcagacctga ggagccacaa tcgagccgtc tcaaaataca taaccaggaa
     6601 agattaaaag caattttttt cttgacaaag gatacgcctt tctctatggt tcattcaaat
     6661 ggtggggcaa agtggataat tgtgggggat aataggtgga agtgttccgg ggacattatc
     6721 accataccat cgactccaag ggtaggatca tcttcccggc aaagctccgt gaggtcttcg
     6781 cagaaaagta tgacaacagg ctggtgatta ccaactggga cggctacctt atggttttcc
     6841 cctatgacga gtggcggatt ctcgaagaaa aagtcatcca tcagtcgctc ctgcgaaaag
     6901 aagtcagggc cttccagcgt tttttcatgt ccggtgccgt cgactgcact ttcgacagcc
     6961 agggaaggat cctcatcccc ccgcccctca gggaattcgc caaactcgag aaagacgtcg
     7021 ttcttgccgg gatgggcaag gtaatcgaaa tctgggacaa agaacgtttt gaggcggcga
     7081 cagatccttc aaaaatagac atggacaagt tcggcgagta catggctgac cttggcatct
     7141 gatcggccgc tcgctgaata gagccggggt cttccatgtt ttcttcctcc tatcatcagc
     7201 ccgtcatggt cgcaggggtc cttcgtttcc tcgcctgcag gcccggcggt gtctatgtgg
     7261 atggtaccgt cggcggcggc ggacatgcag cggctatcct ggaggcaacg gcccccgacg
     7321 gaaggctcat cggcatcgac ttggatgacg aagccctggc cgaagcgcac cgcgtgctgc
     7381 ggcgcggggg gcggttcttc
//
LOCUS       DAOTMX010000041         7322 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_8900_length_7322_cov_3.906839, whole genome shotgun sequence.
ACCESSION   DAOTMX010000041 DAOTMX010000000
VERSION     DAOTMX010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 7322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7322
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8900_length_7322_cov_3.906839"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /locus_tag="P5573_02050"
     CDS             <1..830
                     /locus_tag="P5573_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HRT26879.1"
                     /translation="FAAKAFLDEGVRPENTVGLAFVSDEETGSNKGLKYLLETQRSLF
                     RHTDLIVVPDSGSSDGSKVEVAEKSMLWVRFRTVGKQCHASRPELGRNAFLAASHLVV
                     KLSHLTDSFNFSDPLYEPPVSTFQPTRKDANVPNINTIPGDDVFYLDCRVLPRYPLSE
                     VLEAMRKAADDVERTFDVRIEISPVEQVQAPPPTPTDAPVVKALERAIKDIYGVSAEP
                     VGIGGGTVAAPFRQVGFPVAVWSRIDLTAHQPNESCFIENMLGDAKVYAHLFLQGSD"
     gene            912..1928
                     /locus_tag="P5573_02055"
     CDS             912..1928
                     /locus_tag="P5573_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRT26880.1"
                     /translation="MKPETEDISVKKISFPDNNLLRDLFGERERNLKLIERLGHVKVQ
                     ARGNALTLSGGGIAVNLIENLLLQLYRLLERGYPIYSTDVEYAYRILCENNAVQIEHI
                     FLDTVFISSKKRTISPKSIAQKTYIDAMRKYDMVFCIGPAGTGKTYLAMAMAVAELLE
                     KKVNRIVLARPAVEAGEKLGFLPGDLAEKVNPYLRPLYDALFDMLDFDRASALIHKGI
                     IEVAPLAFMRGRTLNDSFVILDEAQNTTSEQMKMFLTRLGFGSKAVITGDVTQTDLPP
                     DKPSGLIEVEGILSKKEGIKFVYFSEIDVVRHPLVQQIIRAYNHLDRGKKKQKEEPPF
                     TIQK"
     gene            1925..4282
                     /locus_tag="P5573_02060"
     CDS             1925..4282
                     /locus_tag="P5573_02060"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HRT26881.1"
                     /translation="MNRNNVLLKPRNHAGGPIHIPDFLKRVSFQRWLILIVFSLSLSL
                     VLFSDRVVSVPDFFAGAIAGKDLRADRDFPVIDRSATNQKKAEALRTVEPVFDRDEDI
                     ASLLTVRISAAFDGARKSLQQEKKAASPHTNGERESLRSIKQDFELTSGIVLTDREFA
                     VLEKRGFSEEIMGGISRLLNDVFKNHLISRSDFERVESTKGIVIRNIGTGEEEIFRNT
                     YPVIMVEDILPLLKKRAEAILRQEEPTIRAVIVSIVQKAVKPNLVFNREATESRRQLV
                     MKNLKPVYIMVHKNELIVREGDRISPQQMDTIEAYYQARSGSAVMRLSLFLGIFFTVA
                     VFTIVLYCLSKGFLKNLENSNGDLLFLGCIALLQVLVVRIGILVSEAVNHAFPAIPLD
                     ACYYALPFAMGPMIAVVFLNRNIALIFSVFLSFLITFQFDNKMPMFLFSFLGSAVSVY
                     NMDYVNQRSATYRSGLFAGLVNVSVILCITLLSGTPTFPDTFIKLLGGLIGGILSGVI
                     VAGITPLLESAFHYTTNIKLLELSNLNQPILQRMIVEAPGTYHHSMIVASMVEAAAEA
                     INANSLLAKVSAYYHDIGKLTKPLYFIENQIGCKNKHDYLSPKMSSLVIISHVKDGCD
                     LARRYKLGKAITDVIRQHHGTSVVSYFYEKAQKDKDPTIRSIPESEFRYPGPKPQTKE
                     AGLVLLGDVVEASSRSLTEPTPSRIKSLVQTRIRQVLNDGQLDDCELTLRDLNKIAEV
                     FERTLNAIFHHRIDYPNQGVKETDGRRASNGVYDFKPPEKAKGRH"
     gene            4287..4703
                     /gene="ybeY"
                     /locus_tag="P5573_02065"
     CDS             4287..4703
                     /gene="ybeY"
                     /locus_tag="P5573_02065"
                     /inference="COORDINATES: protein motif:HMM:NF014216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HRT26882.1"
                     /translation="MKTAFERLLEHIDCKGREVNVLLLDDEGIRVYNKEYLGRDRPTN
                     VIAFAMREGDFGDLNPDILGDIMISVETAQREAAYAEIPFTEMIDYLMIHGLLHLLGY
                     DHESDRSAGRKMKRKEEELFFSLYGRSIGSGGDSYR"
     gene            complement(4773..6551)
                     /locus_tag="P5573_02070"
     CDS             complement(4773..6551)
                     /locus_tag="P5573_02070"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRT26883.1"
                     /translation="MVRFGLFGFALYLIFSLSCPCPLEAAVTSQQADRVNLAVLFQRE
                     NATLEKTAATEAGKSDAVKSKVVRVSAKEKEAPGVRLITETDAKENAPPSRGAKSNAG
                     KEGKGEALREASGQETLDVALDLLSQSRIFWEKGDVDNALKSLDEAYALILEVNGDPD
                     LSRQKDDLRLMISKMIVQVCASRHTVTTGKQSEIPMIMNADVEKEIRSFQTVERGFFL
                     RSYHRAGMYLPSIQKNLKEAGLPKELAWLPLVESGFQVSVLSRARALGLWQFIPSTGY
                     KYALKRDQWVDERMDVEKSTKAAIAYMKELHDIFGDWLTVLAAYNCGEGKVLRVISKQ
                     HINYLDRFWDLYRQLPQETARYVPRFLATLHIIRDPKKYGMDLSENPEKPIPYEIVKT
                     NKCMRIEDIADKMNVQVETLQMLNSELRLKMTPDSVYNLKVPSGTGVQFAKVADEIPR
                     WEPPPPRRLASKYIKHRVRAGETVGSIAKRYHLSKQDILAVNKVSAKRPLKVGQRIKI
                     PVEKDQDVPVKLAKKRGGKPVVRAAAAEEFVKYRVKRGDTLVSIAAKHNTTVSELKKI
                     NGLTRSKVQTDQIVIIKSNKMARKGS"
     gene            complement(6669..>7322)
                     /gene="prfB"
                     /locus_tag="P5573_02075"
     CDS             complement(6669..>7322)
                     /gene="prfB"
                     /locus_tag="P5573_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139198277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HRT26884.1"
                     /translation="RMYLRWAERRGFKASIIDYQPGEEAGLKSVTVTLEGDYAYGYAK
                     AENGIHRLVRISPFDAGGRRHTSFAAVFVYPEIDEKIQVDIDEKDLRVDTYRSTGAGG
                     QHVNKTDSAVRITHIPTGIVVQCQNERSQHKNKAMAMKVLRSRLYELKEREKNEKLSE
                     IEKTKKEIAWGSQIRSYVLHPYKMIKDHRTNLEVGNVTAVLDGDIDGFIEAYLLKKE"
BASE COUNT         1632 a         2148 c         1868 g         1674 t
ORIGIN      
        1 ttttcgcagc caaagcgttt ctcgacgagg gcgtccgccc tgagaacacg gtcggactcg
       61 ccttcgtttc ggacgaggag acgggcagca acaaaggact caagtacctc ctcgagacac
      121 aacggtcctt gttcaggcat acggacctca tcgtcgtccc cgactcgggg agcagcgacg
      181 ggtctaaggt cgaagtcgcc gagaagagca tgctctgggt aaggttcaga acggtgggga
      241 aacaatgcca cgccagcagg cccgaactgg ggcgaaacgc cttcctggcc gcttcacatc
      301 tggtggtaaa gttgagtcat ctcactgaca gttttaactt ctccgacccg ctgtatgagc
      361 cgccggtcag taccttccag cctacacgta aagacgccaa tgtcccgaac atcaacacga
      421 tccccggaga tgatgtcttc tacttagact gcagggtgct cccccggtac cccctctcgg
      481 aagtcctcga ggccatgagg aaggctgccg acgatgtcga aagaaccttc gacgtcagaa
      541 tcgagatttc cccggtcgaa caggtccagg caccgccgcc gacgccgacg gacgcccccg
      601 tcgtcaaggc ccttgaaagg gccataaagg acatctacgg cgtcagcgcc gaacccgttg
      661 gcatcggggg aggcaccgtg gcagctccct tccgccaggt cggcttcccg gtcgctgtgt
      721 ggtccaggat cgacctcacg gcgcatcaac caaatgaatc ctgtttcatc gagaacatgc
      781 tgggtgacgc caaggtctat gcccacctct tcctccaggg ttcagattag gttggcctca
      841 atcagggtca ttgcgctcaa ccctgtcgag tggtatcatt cgacgcagga cagacagagg
      901 cagaacggaa tttgaagccg gaaactgaag acatctcggt aaaaaagatt tccttccccg
      961 acaacaacct tctccgggac cttttcggcg agagggagag gaatctcaag ctgatcgaac
     1021 gcctggggca cgtgaaggta caggcgcggg gtaacgcctt gactctgagc ggcggtggaa
     1081 tagccgtcaa cctcatcgaa aacctcttgc tccagctcta caggcttctc gagcggggct
     1141 atcccatcta ttccaccgat gtcgaatacg cctacaggat actatgtgaa aacaacgccg
     1201 ttcagatcga gcacatcttt ctcgacacgg tcttcatctc ctcaaaaaaa aggactatct
     1261 ccccgaagag catcgcgcag aagacgtata tcgacgccat gaggaagtac gatatggtgt
     1321 tctgcatcgg gcccgcgggc accggcaaga cctacctcgc catggcaatg gccgtggccg
     1381 aactactcga gaaaaaagtg aacaggatcg tcctggcaag gccggccgta gaagccggcg
     1441 agaaactcgg ctttctgccg ggtgacctcg cagaaaaggt aaacccatac ctcaggccgc
     1501 tttacgacgc ccttttcgac atgctcgact tcgaccgggc gtccgccctg atccacaaag
     1561 ggataataga ggtagcaccc cttgccttca tgcgcggccg gacgctgaac gattccttcg
     1621 tcatcctcga cgaggcgcaa aacaccactt ccgaacagat gaagatgttc ctgacccgac
     1681 tcgggttcgg ttcgaaggcg gtgatcaccg gcgacgtgac acagacggac cttccgccgg
     1741 ataaaccctc cggcttgatc gaggtcgagg gtatcctcag caaaaaagaa ggcatcaagt
     1801 ttgtttattt ttccgaaata gacgttgtca gacaccctct cgtccagcag atcatccgcg
     1861 cttacaacca cctggacagg gggaagaaaa aacagaagga agagcctccc ttcaccattc
     1921 aaaaatgaac agaaacaacg tccttttgaa gccgaggaac cacgcaggcg gcccgatcca
     1981 cattccggac ttcttgaagc gggtgtcctt ccagagatgg cttatcctca tcgtcttcag
     2041 cctgtcgctt tccctcgttc tgttttccga ccgcgtggtc tcggtccccg atttcttcgc
     2101 cggcgcgatt gccgggaaag accttcgagc cgaccgtgat ttcccggtca tagaccgaag
     2161 cgcgactaac caaaagaagg ccgaggcact gaggacggta gaacccgttt tcgacagaga
     2221 cgaagacata gcatctcttc tgactgtaag gatctcggcc gccttcgacg gagcgcgcaa
     2281 atcccttcag caggagaaaa aagcagcgtc gccccacact aacggtgagc gtgagtcatt
     2341 acggagtatc aagcaggatt tcgagttgac ttccggcatt gtcttgacgg accgggaatt
     2401 cgccgttttg gaaaagaggg ggttctcgga agagatcatg ggcggcatct cgaggcttct
     2461 gaacgacgtc ttcaagaatc atctcatcag ccgcagtgat ttcgagagag tcgaatccac
     2521 gaaaggtatc gtcatcagga atatcggcac tggcgaggaa gaaatattca gaaacaccta
     2581 cccagtcatc atggtcgaag acatcctccc cttgctgaaa aaacgagcgg aagcgatcct
     2641 ccgacaggag gaaccgacga ttcgtgccgt gatcgtttcc atcgtccaaa aagccgtgaa
     2701 gcccaatctg gtattcaaca gagaagccac cgagtcgcgg cgacaactag tgatgaagaa
     2761 cctcaaaccg gtctacatca tggtccataa gaacgaattg atagtcaggg aaggcgaccg
     2821 gatctctcct cagcagatgg ataccatcga ggcctactac caggcgcgga gcggaagcgc
     2881 cgtcatgagg ctgtcccttt ttctcggcat cttctttacg gtcgcggtct tcaccattgt
     2941 cctttactgc ctttcaaagg gcttcttgaa gaatctcgaa aacagcaacg gtgaccttct
     3001 tttccttggc tgcattgccc tcctgcaggt cctcgtggtc aggataggta tcttggtctc
     3061 cgaggcagtc aaccatgcct tcccggctat ccccctcgac gcttgctatt acgcgctgcc
     3121 tttcgccatg ggaccgatga tcgccgtagt gttcctcaac cgaaacatcg cactcatctt
     3181 ctccgtcttc ctctcattcc tcattacctt ccagttcgac aacaagatgc ccatgtttct
     3241 cttttccttc ctgggaagtg ccgtctcggt ctacaacatg gattatgtca accagcgctc
     3301 cgccacttac aggtccgggc ttttcgccgg cctggtcaat gtgtctgtca tcctgtgcat
     3361 caccctcctg tcggggacgc cgactttccc cgacaccttc atcaaactcc tcggcggtct
     3421 catcggcggc atcctgtcgg gggtcatcgt tgccgggata acgccgctcc tggaatcggc
     3481 cttccactac accacaaaca tcaaactcct ggagctgtcc aacctgaacc agccgatctt
     3541 gcaacgcatg atcgtagagg ctccgggcac ttaccatcac agtatgatag tcgcgtctat
     3601 ggtagaggcc gcagcggagg cgatcaacgc aaattcactc ctcgcaaagg tgagcgccta
     3661 ctaccacgac atcgggaaac tgacgaagcc gctgtatttc atcgaaaatc agataggctg
     3721 caagaacaaa cacgactacc tatcgcccaa gatgagcagt ctcgtgatca tctctcatgt
     3781 caaagacggg tgcgacctcg ccaggcgcta caagctcgga aaggccatca ccgacgtgat
     3841 ccgccagcac cacgggacga gtgtcgtcag ctacttctac gaaaaagcgc agaaggacaa
     3901 ggaccccaca atccgttcga ttcccgagag tgaattccgt tacccgggcc cgaaaccaca
     3961 gacaaaggag gcggggctgg tactgctcgg cgacgtcgtg gaggcatctt ctcggtccct
     4021 gacggaacct actccttcga ggatcaaaag tctcgtccag acgcggatca ggcaggtgtt
     4081 gaacgacggc cagctcgacg attgcgaact gacactccga gacctaaaca agatcgccga
     4141 agtcttcgaa aggacgctga acgccatatt ccatcaccgt atcgactatc ccaaccaggg
     4201 agtcaaggaa accgacgggc gaagggcgtc aaacggtgtt tatgatttta aaccgccaga
     4261 aaaagctaaa ggtagacatt aagcgggtga agaccgcttt cgaacgcctt ctcgaacata
     4321 tcgactgcaa gggtcgggaa gtgaacgttc tactcctcga cgacgaggga atccgggtgt
     4381 acaacaaaga atacctcggg cgcgacaggc cgacgaacgt catcgccttc gccatgcggg
     4441 agggagactt cggagacttg aacccggaca tcctggggga catcatgatc tccgtggaga
     4501 cggcgcaacg agaggcggcc tacgccgaga tccccttcac tgagatgatc gactacctga
     4561 tgattcacgg cttactccat ctcctcggct acgaccatga gtcggaccgt tcggcgggcc
     4621 gaaagatgaa aaggaaagaa gaagaacttt ttttctctct ctacgggcgt tcgatcggtt
     4681 ccggcggcga ctcataccga taaaaaaagg cggatatcct ccgccttcct tcggctcata
     4741 gccccattat ccatcctctt accccagacg tctcaggaac ccttcctcgc catcttgttg
     4801 ctcttgatta tcacgatttg atcggtctgt actttacttc tcgtaaggcc gttgatcttc
     4861 ttgagttcgg aaacggtcgt gttgtgcttc gccgctatcg aaacaagggt atctcccctc
     4921 tttaccctgt atttgacgaa ctcttcggcg gcagccgctc ggacgaccgg tttaccccct
     4981 ctcttcttcg ccaacttgac gggcacgtct tgatcctttt ccactgggat cttgattctc
     5041 tgcccaacct tgagaggtct tttcgcggaa actttattga ctgccaggat atcctgcttc
     5101 gacaggtgat acctcttagc tatactgcct acggtttctc cggccctgac acggtgcttg
     5161 atgtacttgc ttgccaatcg gcgtggaggc ggcggttccc accgcgggat ctcgtccgcc
     5221 accttggcaa attgcacacc cgttcccgat gggaccttca ggttataaac gctgtcaggc
     5281 gtcatcttca gcctaagctc ggagttcagc atctgaaggg tttcgacctg aacgttcatc
     5341 ttgtcggcaa tatcctcgat cctcatgcat ttattggtct tgacgatttc atagggaatc
     5401 ggcttctcgg gattttccga caggtccatc ccatattttt tggggtctct gatgatgtgc
     5461 agggtagcaa ggaatctcgg cacataacgc gccgtctctt gggggagctg acggtataga
     5521 tcccagaaac ggtctagata attgatgtgt tgcttcgaga tgactctcag gaccttacct
     5581 tctccacaat tgtaggcggc gagcacggtc aaccaatcgc cgaaaatgtc gtggagttcc
     5641 ttcatgtagg caatggcggc cttcgtcgat ttttcgacgt ccatcctctc atcgacccac
     5701 tggtccctct tcagagcgta tttgtaaccc gtggaaggga tgaattgcca cagtcccaac
     5761 gcccttgccc tcgagagcac actgacctga aaaccgctct cgacgagcgg aagccaggcg
     5821 agctctttcg gcaaccccgc ctccttcagg tttttttgta tggaaggaag atacatcccc
     5881 gcccgatggt aagacctgag aaaaaacccc cgctcgacgg tctggaaaga gcggatctct
     5941 ttttccacat cggcattcat gatcatcggg atttcgcttt gtttgccggt tgtgacggtg
     6001 tggcgggagg cacagacttg aacgatcatc ttggagatca tcaacctcag gtcgtccttc
     6061 tgtcgggaca ggtcgggatc cccgtttact tcgaggatga gtgcgtaagc ctcgtccaga
     6121 gactttaggg cattatcgac atcgcccttc tcccagaaaa tccgcgactg actcaagagg
     6181 tcgagcgcca cgtccagcgt ctcctgaccg cttgcctctc gcagggcttc tccctttccc
     6241 tctttgcctg cgttggattt cgcaccccgt gacggtgggg cgttctcttt tgcgtccgtc
     6301 tccgtgatca gcctgacccc cggcgcctcc ttctctttgg cggagacccg gacgacttta
     6361 gacttcaccg catcgctttt gccggcttcg gtcgctgctg tcttttcgag tgttgcattc
     6421 tctctttgaa aaaggacggc gagattcacc cggtctgcct gctgcgatgt aacagccgct
     6481 tcgagaggac acgggcagga cagagaaaaa atcaagtaca gcgcaaaacc gaagagtccg
     6541 aatcgcacca tcgcacacct cctggtaaaa gactgcttcg ctcaaaggcc gcagagacga
     6601 taatacattt ttacccttat gaaaaggaaa atatgcacaa ctttttccct cgtcttttcc
     6661 cgtaagggtc attctttttt caaaaggtag gcttcgatga agccgtctat gtccccgtcg
     6721 agcacggccg tcacgtttcc gacctcgagg tttgtgcggt ggtcttttat catcttgtaa
     6781 ggatggagga catacgaacg tatctgactt ccccaggcga tctccttttt cgtcttttcg
     6841 atctcgctca gtttttcgtt cttctccctt tctttgagct cgtagagccg tgaccgtagt
     6901 accttcatgg ccatggcctt gttcttgtgc tgcgacctct cgttctggca ttggacgact
     6961 atgcctgtgg ggatgtgcgt gatcctgacg gccgagtccg tcttgtttac atgctggcct
     7021 ccggcgcccg tcgaccggta ggtgtcgacc cgcagatcct tctcgtcgat gtcgacctgg
     7081 atcttttcgt ctatctccgg gtagacgaaa acggcggcga aggacgtgtg tctcctgccg
     7141 cccgcgtcga aaggtgatat tctgaccaag cggtgtatgc cgttttccgc cttggcatac
     7201 ccgtatgcat aatccccctc gagggtcacc gtgacgcttt tcagccctgc ttcctcgccg
     7261 ggttgataat cgatgatgct tgccttgaaa ccccttcttt ccgcccatct caggtacatg
     7321 cg
//
LOCUS       DAOTMX010000042         7165 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_9115_length_7165_cov_3.961463, whole genome shotgun sequence.
ACCESSION   DAOTMX010000042 DAOTMX010000000
VERSION     DAOTMX010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 7165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7165
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9115_length_7165_cov_3.961463"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..>977
                     /locus_tag="P5573_02080"
     CDS             54..>977
                     /locus_tag="P5573_02080"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HRT26885.1"
                     /translation="MKDGKEILALFPHKDRKRWVLWTPSGYYDAAPGAEELIGWHVNN
                     GKDAAADFFPASRFRAAYYRPDVVAKVIETLDEREAVRLADEESGRRKQAVTVREMLP
                     PVVTIVSPADGAEVSIPEVTVKFSVRTPSGEPVTAVKALVDGRPAAAERGVKIVEREG
                     DVQELRVTIPERDVEIAVIAENRYTASEPATVRVRWKGAPREEFVVKPRLYVLAVGVS
                     NYADKSLRLSFAAKDARDFVTVMDVQKGRLYRDVVVKVMTDEGATKDEILDGLDWLQK
                     ETTSKDVAMVFLAGHGVNDPSGVYYYLPVNAD"
     assembly_gap    978..987
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <988..1500
                     /locus_tag="P5573_02085"
     CDS             <988..1500
                     /locus_tag="P5573_02085"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HRT26886.1"
                     /translation="MRFILAHAQKSLVGALEMLVAPVNADTDRLKRTGVAFSDIKNTV
                     ASLAGKAVVFVDTCHAGSVMGARRGVADITAVVNELASAENGAVVFASSTGRQYALED
                     AAWGNGAFTRALVEGIGGKADYTGKGKITVNMLDLYLSERVKELTKGRQTPTTTKPHT
                     VPDFPIAVTR"
     gene            complement(1561..2673)
                     /locus_tag="P5573_02090"
     CDS             complement(1561..2673)
                     /locus_tag="P5573_02090"
                     /inference="COORDINATES: protein motif:HMM:NF017197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HRT26887.1"
                     /translation="MGKGRRFKKVAVILAVFSAIVFVAGVLLLHNANRIIKAELESAL
                     GRAFSVQEVGLSWGEVKASHIELKNKAGKTVFTTAGLTIQADFLGLLKRDFVISHLTI
                     NEPYVFMEIDHRGNLVNPSLPAGEGGKGGKEKKPLPPFLIKKVLITKGSLDYLDRQPR
                     AGSAFIKVRDIDVELQGVALPPGGGESQYTLGAVITGIKSSGTIKSKGKVRLKDEKIE
                     DAEAKVEVRNVDIIPFRPYFQKRGVNITRGLLDLDMDVKVRARKLHAPGRALLRDLDF
                     KSGPGIGNKFRGVPLSIAVATLKNDKNEITVNFVLEGDLNNPKFSLREGVADKLSLAV
                     ANKLGISVKDIGKSVIKTGTEGVKKGVEGIKGVFNN"
     gene            2817..4139
                     /locus_tag="P5573_02095"
     CDS             2817..4139
                     /locus_tag="P5573_02095"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HRT26888.1"
                     /translation="MSRIRYKGGMNKHRIFIADSRSMKDVQDRSVHLVVTSPPYWQLK
                     DYDSARQIGFHDTYEEYINNLNLVWKECHRVLHDGCRLCVNIGDQFARSVYYGRYKVI
                     PIRTEIIKFCETVGFDYMGAIIWQKVTTCNTTGGATVMGSYPYPRNGILKIDYEFILL
                     FKKPGNPPPVDRTAKEQSRLSAEEWNEYFAGHWHFPGEKQDKHLAMFPEELPRRLIRM
                     FSFVGDTVLDPFLGSGTTSLAARKLERNSVGYEINESFLPVIRKKIGADQPDIFSETA
                     VEIIKPASQPIDYMQEIKKLPYVFHDPVRFDKKVDPRKMTFGSKIEADGNGNEQYLRV
                     KRVITPHTMELTNDVVIRLIGVGAKAGKEREAVEWLWDRLKGQRLCLKFDQTKYDEDN
                     RLLSYLYLKNKTFINLHYLRSNLVRLDETFPFKYRDRFRAILKEEIEP"
     gene            complement(4173..4913)
                     /locus_tag="P5573_02100"
     CDS             complement(4173..4913)
                     /locus_tag="P5573_02100"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRT26889.1"
                     /translation="MDNIAGKVVVITGASSGLGEAAARLLSAQGASVVLGARRADRLR
                     SLAEELTANGGKALAVVTDVTQVDQVKALVDAAVQTFGRIDVMINNAGLMPSSLLERL
                     RVEDWDRMVDVNLKGVLYGIAAALPYMKQQKSGHFINVSSVAGHKVRPGGAVYAATKH
                     AVRALSEGLRQEVKPYNIRTTVISPGAVATELPNSITDPDFAAASRRLYDVAIPAQSF
                     ARAVAFAISQPEDVDVNEILFRPTRQEF"
     gene            complement(5159..5671)
                     /locus_tag="P5573_02105"
     CDS             complement(5159..5671)
                     /locus_tag="P5573_02105"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRT26890.1"
                     /translation="MTDELMSALAKTGLFQGLTADQVRTFLTIAGKVAFRKDDIIMEE
                     GKTGDTMYIILEGSVEVVKRLTMKGFDDDSDQKSKVFTKLEAENHSIFGEVALLEESE
                     RTATVRAISDCLLYEIRRDAFLRLAESNPELGFKVILNLAKIMGSRLRKADEDVVKLT
                     TVLSLVLKET"
     gene            complement(5740..6909)
                     /locus_tag="P5573_02110"
     CDS             complement(5740..6909)
                     /locus_tag="P5573_02110"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HRT26891.1"
                     /translation="MKLVAKTRTVTKALAKERFFFVAPITLAVIIIASVGVFYAERHM
                     DAATIHSIWDGVWWAFVTICTVGYGDKVPLSFAGKIIGIFLMISGIGLLSMLTATVAS
                     VLVEQKIKEGKGLETIKDKGHIVVCGWNNNTEEVIRGLTAGTSGSLPIVLINELPIDE
                     MDILRAKYGKQNVIFLRGDFVHEEVLLRANITRAGFALIMADFSGTHPRDRADDRTIL
                     GALAVKSLAPKIKVIAELLDGESKPHLKRANVDEIIVRGEHIGSLLAGAVRSPGLSKV
                     YAGLLSFEGAGMLKRDEIPKQYVGHTFGELSAYYRGKRKAILIGLLKESQGVKVEDVL
                     SDDTSAIDLFIREKLKASNKGVLQREEATKVIINPDDHHVITADDHAVILSSPES"
BASE COUNT         1569 a         2042 c         1946 g         1598 t
ORIGIN      
        1 acgggaaggt ggctgttgct gctattgctg acggaacgat caggtggtac cggatgaagg
       61 acgggaagga gattctcgcg ctctttcccc ataaagacag gaaacgctgg gtcttatgga
      121 ctccctccgg ttactacgac gccgcccccg gcgccgagga gctcatcggc tggcacgtga
      181 ataacggcaa agacgccgcc gccgacttct ttcccgcgtc ccggttcagg gccgcctact
      241 accgccccga cgtggtggcg aaagtcatcg agacgttaga cgagcgcgag gcggtaaggc
      301 tggccgatga ggagtcgggg aggagaaaac aggcggtcac cgtccgggaa atgcttcccc
      361 ctgtcgtaac gatcgtgagc cctgccgacg gggccgaggt ctccatcccg gaggtgacgg
      421 tcaagttcag cgtccgtacc ccctccggtg agcccgtgac ggccgtaaag gcgctggtgg
      481 acgggaggcc ggcggccgcc gagcgcggcg tgaagatagt agagagggaa ggggatgtgc
      541 aggaactccg ggtcacgatc ccggagaggg acgtggagat cgccgtgatc gccgagaata
      601 ggtataccgc gagcgaaccg gcgaccgtta gggttaggtg gaagggtgcg ccgagggaag
      661 aattcgtcgt caaacccagg ctctatgtgc tcgccgtcgg cgtcagtaat tacgcggaca
      721 agagcctgcg gctttccttc gccgccaagg acgcccggga ctttgtgacg gtcatggatg
      781 tgcaaaaggg caggctgtac cgggacgttg tggtaaaggt catgaccgat gagggtgcga
      841 ccaaggacga gatactggac ggcctcgatt ggctccagaa agagacgacg agcaaggatg
      901 tggcgatggt atttcttgcc ggccacgggg tgaatgaccc gagcggggtg tattactatc
      961 ttcccgtcaa tgcggacnnn nnnnnnnatg cgattcattc tcgcgcatgc tcagaaaagc
     1021 ctcgtaggag ccctcgaaat gctcgtagct cccgtcaatg cggacacgga ccggctgaag
     1081 aggaccggcg tcgccttctc ggacatcaag aacaccgtcg cctcccttgc cggtaaggcc
     1141 gttgtctttg tcgatacctg ccatgcgggc agtgtcatgg gtgcgcggag gggagtggcc
     1201 gacataacgg ctgtcgtcaa tgagctggcg agcgccgaaa acggcgccgt ggtcttcgcc
     1261 tcctctacgg gcaggcagta cgccctcgag gatgcggcat ggggtaatgg ggcgttcacc
     1321 agggccctcg tcgagggcat aggcggaaag gcggactaca cgggcaaggg caagatcact
     1381 gtgaatatgc tcgacctcta tctctcggag cgggtgaagg agctcacaaa gggaagacag
     1441 acgccgacga cgaccaagcc ccatacggtc cccgacttcc ccatcgccgt gacgaggtga
     1501 tcgaggcctc cgcgaaatac gctgaagggt gcttttgaag gagcggccgc ccggctacgg
     1561 tcagttattg aatacgcctt taatgccctc tacgcccttt ttgacaccct cggtgcccgt
     1621 cttgatgacg gattttccta tgtctttcac cgaaatgccg agtttgttgg ctacggcaag
     1681 ggatagcttg tcggcgacac cttccctgag gctgaacttc gggttgttta ggtcaccttc
     1741 gaggacaaaa ttcaccgtaa tctcattttt atcgttcttg agggtggcga cggcaatcga
     1801 gagcggcacg ccgcggaatt tgttcccaat gccggggccg ctcttgaaat cgaggtcccg
     1861 cagcagggcc cgccccgggg catggagttt ccgggcacgg accttcacgt ccatgtcgag
     1921 gtcgagaagt cccctggtta tattcacccc tctcttctgg aagtaaggcc taaaggggat
     1981 gatgtcgaca ttgcggacct cgaccttcgc ttcggcatct tcgattttct cgtctttgag
     2041 tctgaccttc cccttgctct ttatcgtccc tgaactcttg atgcccgtga taacggcgcc
     2101 cagggtgtac tgcgattcgc ccccgcccgg cgggagggcg acaccttgaa gctctacgtc
     2161 gatatcccgc accttgatga aagcggatcc cgcccgcggt tgccggtcga gataatcgag
     2221 agaacccttt gttatcagga ccttcttgat aagaaacgga ggcagaggtt tcttttcctt
     2281 tcctcccttt ccaccttctc ccgccggcag tgaaggattt accagattac cccggtggtc
     2341 tatttccatg aatacatagg gttcgttgat ggtcaggtgt gagatgacga aatccctttt
     2401 cagcaggcct aaaaagtctg cctggatggt caggccggcg gtcgtgaaca ccgttttccc
     2461 cgccttgttt ttgagctcga tatgggaggc cttaacttct ccccacgaaa gtccgacctc
     2521 ctgaacggaa aaagccctcc caagggccga ttcgagctct gccttgataa tcctgttggc
     2581 attatgaagg agcagaaccc ccgccacaaa aacgattgcg ctgaaaacag cgaggataac
     2641 cgctaccttc ttaaaccgtc ttcccttccc catcgagctt ttccttttag gtttcagata
     2701 gacaaacctg acccctcttc ctccagtgaa aggccttata tccctttttt atccctattt
     2761 cagtcagttt ctgcatcatg cgcaagcctg aattgtcacc cttcgatatt gaatagatga
     2821 gtaggatcag gtataaaggc ggcatgaaca agcaccggat ttttatcgcc gattcccgct
     2881 ccatgaagga tgtgcaggat cggtccgtcc acctggtggt cacgtcgccg ccttactggc
     2941 agctcaagga ctacgacagc gcccgtcaga ttggctttca cgacacctac gaagaataca
     3001 tcaacaacct caacctcgta tggaaggagt gccatcgcgt actgcacgac ggttgtcgcc
     3061 tctgtgtcaa catcggagac cagtttgccc ggtccgttta ttacggtcga tacaaggtaa
     3121 taccgatcag gaccgagatc attaaatttt gcgaaaccgt cgggttcgat tatatggggg
     3181 ccatcatatg gcagaaggtg acgacctgca acacgacagg cggcgccacg gtcatggggt
     3241 cgtacccgta tccgagaaat gggatcctca agatcgatta cgaattcatc ctgctcttca
     3301 aaaagcccgg gaatccccca cctgtcgaca gaacggccaa agaacagtcc aggttgtcag
     3361 cagaagagtg gaatgaatac ttcgccggcc actggcattt tcccggggag aaacaggata
     3421 agcacctggc catgtttccg gaagagcttc ccaggaggtt gatcaggatg ttcagctttg
     3481 tcggggacac ggttctcgac ccctttttgg ggagcggcac aacctctctt gcggcaagga
     3541 agctggagag gaactctgtc gggtatgaga tcaacgaatc atttctaccg gtaatcagga
     3601 aaaagatagg ggccgaccag ccggatattt tcagcgagac ggctgtcgaa atcatcaaac
     3661 cggcaagcca acctatcgat tacatgcagg aaataaagaa actgccctat gtatttcacg
     3721 acccggtgcg cttcgataag aaggtagatc ctcggaaaat gaccttcggt tccaaaatcg
     3781 aagccgacgg aaacggcaac gaacagtatc tacgcgtgaa acgcgtaata accccccaca
     3841 cgatggaact caccaacgat gtagtcatca ggttgatcgg tgtcggggcc aaggcaggca
     3901 aagaacggga ggccgtggag tggttgtggg atagactgaa ggggcaaagg ctttgcctga
     3961 aattcgacca gaccaagtat gacgaagaca accgtcttct ctcttatctg tacctcaaaa
     4021 acaagacctt catcaatctt cactaccttc gctccaatct tgtccggttg gacgagacgt
     4081 tccccttcaa gtatcgcgac aggttcaggg cgatccttaa ggaggaaatc gagccgtgaa
     4141 aaagctcagg atgcagaggc cttctagcga gactaaaact cctggcgcgt tggccgaaac
     4201 aggatctcgt tcacatccac gtcctccggt tggctgatgg cgaaggcgac cgcccgggca
     4261 aacgactggg caggaatcgc aacgtcatag agtcgacgcg aggccgcggc gaagtcaggg
     4321 tcggttatgc tgttaggcag ttccgtggct actgcgcccg gcgaaatcac ggtggtgcgg
     4381 atgttgtatg gtttgacttc ctgccgtagt ccctccgata gggcccgcac ggcgtgcttg
     4441 gtcgctgcat agacggcgcc gccggggcgt accttatggc ccgccacaga ggaaacattg
     4501 atgaagtgtc ccgatttttg ctgcttcatg tacggcaggg cggcggcaat gccgtacagc
     4561 acgcccttta ggttcacgtc gaccatccgg tcccagtcct cgaccctgag gcgttcaagg
     4621 agcgacgacg gcatcagccc ggcgttgttg atcatgacat cgatgcggcc gaacgtctgc
     4681 acggccgcgt ccaccaatgc cttaacctgg tcgacctggg taacgtcagt cactacggcg
     4741 agcgccttac ccccgttggc agtcagctct tctgccaatg accggagtcg gtcggcacgc
     4801 cgagcgccga gcacgacgct cgcaccttga gcggagagca accgggcagc cgcctcgccc
     4861 aacccgctgc tcgcgcctgt gatcacaacg acttttcctg cgatgttatc catggaacct
     4921 ccttctgcat ctcttctcga tcctgatcaa tcttgacgtc cccgtaaaaa gtccaaaaac
     4981 gtcatgccgg acttgattaa gcctgccccg tactcgatac gggggcatcc agaacatatt
     5041 gaatatactg gattccggcg ttcgccggaa tgacgaaacg cgtaattctc gactttttac
     5101 gagtccatca atctttattc ggtgtctaaa aaacccttga cccgtatgaa cgacctcgtc
     5161 atgtctcctt caagaccagg ctcaaaacgg tggtcagctt gaccacgtcc tcatccgctt
     5221 ttcgcagccg cgaccccatg atcttcgcca gattcaggat taccttgaat cccagctcag
     5281 ggttgctctc tgccagcctc aaaaaggcgt ctctccgaat ctcgtatagg aggcaatccg
     5341 aaatggcccg gaccgtcgcc gttcgctccg actcttccag cagagcgacc tctccgaaga
     5401 tagaatggtt ttcagcttcc agctttgtga agactttgct tttttggtca gaatcgtcat
     5461 cgaagccttt catggtcaac ctcttgacca cctccaccga cccctcgagg atgatataca
     5521 tggtgtcgcc tgtcttgcct tcttccatga tgatgtcatc cttgcggaag gcgacttttc
     5581 cggcgatggt caagaacgtt cgaacctgat cggccgttaa accctggaac aggccggttt
     5641 ttgcaagggc gctcatcaat tcgtccgtca ttttttcccc gaccttcctt gtgaccggcg
     5701 atcctctgtc tctcccatat gctgtcgtcg gctctaagac tacgattccg gggacgacag
     5761 aatcacggca tggtcatcgg ctgtgatgac atggtggtca tcgggattga tgatgacctt
     5821 cgtcgcctcc tcgcgttgca acacgccctt gttggacgcc ttgagttttt cccgtatgaa
     5881 gaggtcgata gccgaggtgt cgtcgctcag tacgtcctca accttgaccc cctgcgattc
     5941 cttcaggagg ccgatcaaaa tcgccttccg cttgccgcgg taatacgccg aaagttctcc
     6001 gaaggtatgg ccgacgtatt gtttcggtat ctcgtcccgc ttcaacattc ccgcaccttc
     6061 aaaggataaa agaccggcat acaccttgga gagacccggc gaccgcacgg caccggcaag
     6121 aagagaccct atgtgttccc ccctgacgat tatttcatct acattggcgc gcttaaggtg
     6181 aggcttgctt tccccgtcga gaagctcggc gatgaccttg atcttcggcg ccagggactt
     6241 gacggccaag gcacccagga tcgtcctgtc gtccgcccta tccctgggat gggtcccgga
     6301 aaagtcggcc atgatgagcg caaatccggc cctcgtgata ttcgccctca agaggacctc
     6361 ctcatgaacg aagtcgcccc tcaagaagat gacgttctgt tttccatact ttgcccgcag
     6421 aatgtccatc tcgtcgatgg gaagctcatt gatcaagacg atgggcaagc tacccgacgt
     6481 cccggcagtc agcccccgta tgacttcttc ggtgttgttg ttccagccgc agacgacgat
     6541 gtgcccctta tccttgatgg tttccaatcc cttcccctcc tttatctttt gctccacaag
     6601 gacggacgca acggtcgccg tcaacatgga cagcagtcca atgcccgaaa tcatcaagaa
     6661 gatcccgata atctttcccg caaacgacag ggggacttta tcgccgtacc ccacggtgca
     6721 gatggtcacg aaggcccacc acaccccgtc ccagatagaa tgtatggtgg cggcatccat
     6781 atggcgttcc gcgtaaaaaa cgccgaccga ggcaatgatg atgacggcga gcgttatggg
     6841 cgcgacgaag aagaaacgct cttttgctag agccttcgtg accgtcctgg tcttcgcaac
     6901 aagtttcatg ggtcaatccg gtgggaacgg tgccgatttg ccgtcagtat acaaatcttc
     6961 ttgcgtatga cacccattca attcaggaca tgttgatttc accagttacc tattttttac
     7021 ggcgaaagta acgacgagtc gagtcccgtg ccttcaagcc cgggactcga acgacttgtc
     7081 cggctcttac ttgactttgc agcttttgca ttcagatcca aaaacttctt atttcgtctt
     7141 tccaacaaag gttgcattca cttta
//
LOCUS       DAOTMX010000043         7129 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_9174_length_7129_cov_3.715437, whole genome shotgun sequence.
ACCESSION   DAOTMX010000043 DAOTMX010000000
VERSION     DAOTMX010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 7129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7129
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9174_length_7129_cov_3.715437"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="P5573_02115"
     CDS             complement(<1..315)
                     /locus_tag="P5573_02115"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HRT26892.1"
                     /translation="MTDRLDYRSDVTDMRLTTPQSRRDRIIAGLILAALSWAVLLILY
                     FGNFLESYELRTYDQLCRFDARDAGKPRDTVLVAVDQGSLEAAGGQGINWPWPRQMYA
                     PIV"
     gene            complement(328..1026)
                     /locus_tag="P5573_02120"
     CDS             complement(328..1026)
                     /locus_tag="P5573_02120"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HRT26893.1"
                     /translation="MTKTIPEEDFEKYLRPTWFLDSDEPSVVEFARQAVGNAKDDREK
                     GVRLFYAVRDGIRYNPYDIDLTRRGLKASVILARREGYCVAKAIVLAAAARAVAVPSR
                     LGFADVRNHLTTERLRSLMNTDVFFYHGYTELFLNGRWVKATPTFNISLCERFGVKPL
                     EFDGLSDTLFHEFDSKGNRHMEYVNDRGAFADLPYETIVESFRTHYPGFFKEDGTMVT
                     GNFEKEALEDHGKA"
     gene            complement(1071..3773)
                     /locus_tag="P5573_02125"
     CDS             complement(1071..3773)
                     /locus_tag="P5573_02125"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HRT26894.1"
                     /translation="MPLLTLYFQLHQPFRLHPDREEFLWDQLNKDVFLKVSEKCYLPA
                     TRLFIELIKQYPDFKITLSMSGTFLEQAELYRPEVIKALRDLREAGEARHQVEFLEET
                     YYHSLASLFADPEKREFREQVVMHREIMKRLFGVLPASFRNTELMYNNEIASVVADMG
                     YKAMLCEKRDDMFLSGHRPISPNAVFRAKGTQLIVIPRNRELSDDVAYRFPHHPITAD
                     EYARNIALIDGEAVLLGYDFEHIGEHIWEDKGIFEFWRRLPEALAKHPNVIPANPTEV
                     AERFRNAECPVADIHGLSTSSWADAGRDTFGWLGTMTQYDIFKDIEGMEKEVKKAGGA
                     ILRKWRHLTTSDHIYFLHERVGEDYAVHSYFNPYGGSITQATHVLTRKIDMLQVALRQ
                     FPTLKKKEKTAVLIVTPETGRLPEDMGVLAKYISGKSGGQGEVISALCEGLIDRGIEV
                     HLATLNLKKRFQREAQLSESQWREIRYKIDPDKIHLVSSAVFANLLSAYAGNTLLTAA
                     EFQRELTNQIIKEVSAKSEGKLIVHSHDWMAGGVVTAHAKYAGIPILHTVHNVFTGHV
                     PLEMLMGVELDHFTEHLYFSEEYGKICVDCQATAIKSATLINFVGHRFLQEVVEDYFM
                     DRPIVPHSVRKEVKEKYYAGAALSIMNAPSLAMYPERCGYCVRNYGPDDDIFEAKRIN
                     RVEFQKRTGLIVNPDAILFYWPSRLDPSQKGVEILEDIAFKFCIEHGDVQIAIVGSGV
                     GNDRTHEEILGRIAWASGGKIVYYPFSEELSMLGYAAASDVFGCSLYEPCGQIDQIGN
                     LFGATATNRDTGGYHDKIRELRLATDGFPQDVGNGFLFRDYDSGGLWYGMEKSVQFHR
                     RPAEVREPQIRRIMKEAREKYSLDSMVNSYIAVYERLNGGKPLA"
     gene            4176..4856
                     /locus_tag="P5573_02130"
     CDS             4176..4856
                     /locus_tag="P5573_02130"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HRT26895.1"
                     /translation="MCGRFVMVSPSYVISEVFCLKEEPQDPLPSYNISPGQSVYAVVR
                     DERDETNRLARFRWGLVPSWAKDPSVGNRMINARAESVAERPSFKEAFRDRRCLVVAD
                     GFYEWQKTGRAKVPFFIRLKSGRPFGMAGLYETWVSPAGKILRTCTIITTRANDLLSP
                     IHDRMPVIVHREKQALWLDRSVRDKKHLIDILKPFPPEEMDAYRVSGAVNSPLNDNPA
                     LILPIPDS"
     gene            complement(4857..6299)
                     /locus_tag="P5573_02135"
     CDS             complement(4857..6299)
                     /locus_tag="P5573_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT26896.1"
                     /translation="MLTLMPALRHQIIVFAIVFMLLPVKHASAEYYAVPGVIDTRTTF
                     SDGAYTPQELADLAMQKGLRILVINDHDRVAMEYGLPPLRHILKYRIEHNSINLKGAD
                     HYLKAIEEVEKKHPDMVIIPGSETTPFYYWSGSLLNGNLTANNPERRLLTVGLSKAPD
                     YENLPILHNGGKAGYATVLVLLVAALVLSLLMVRRKGMFRVAGILLGLSAVLLADRLL
                     STDSSPYDPYHGDQGMAPYQLVIDYVTARSGLTFWNYPETKSGIRKLGPIMVNTPPYP
                     QVLTETKGYTGFSALYGDNITLTEPGNIWDLALKEYCSGFREHPPWGIATSDYHREGE
                     SGEKLGNYQTVFLLKEFTKKEVMDAMKSGRMYASRGNFPHVPKLLEFSVSSLDGRSTA
                     ISGQEILLTEKPTVKVSISLEGKASRPVKVRVIRSGQVIKAVESPLPVSLDFQDDCFQ
                     PGEKVYYRLDMHGDGAIVSNPIFVRFKSPE"
     gene            6480..6758
                     /locus_tag="P5573_02140"
     CDS             6480..6758
                     /locus_tag="P5573_02140"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRT26897.1"
                     /translation="MKELAKELRRKYKVSEIIVFGSYARGDLNEGSDIDLLIVGDFKE
                     KFHKRIASILGLTDLPIEALCYTEEEFREMITNNRGFISEVLKEGVAI"
     gene            6794..>7129
                     /locus_tag="P5573_02145"
     CDS             6794..>7129
                     /locus_tag="P5573_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRT26898.1"
                     /translation="MSNSRSYEIVIEPKGTWKALQWGELWQYRELLRFLTVRDIKVRY
                     KQTVLGGLWAILQPFMSMVVFTVFFGMLAKVPSDGLPYPIFVYTALLPWQFFSNGISN
                     AGNSLVASSN"
BASE COUNT         1542 a         1901 c         2007 g         1679 t
ORIGIN      
        1 gacgatcggc gcgtacatct gacgcggcca gggccagttt atcccctgtc cgccggccgc
       61 ctcgaggctg ccctggtcca ctgccacaag aacggtgtcc cggggttttc cggcatcacg
      121 ggcatcaaac cggcagagct ggtcgtaggt cctcagttca taggattcga gaaaatttcc
      181 gaaatacagg ataaggagaa cagcccagga aagggcagcg aggatcaacc cggcgattat
      241 cctgtcccgt cggctctggg gagtcgtgag tcgcatgtcc gtcacgtcac ttctgtaatc
      301 taaacggtcg gtcaatcgac accgcggtca ggcctttccg tggtcctcca gggcctcttt
      361 ctcgaaattg ccggtgacca tcgtcccgtc ttctttgaaa aaaccgggat agtgcgtcct
      421 gaaggattcc acgatcgttt catagggaag gtcggcaaaa gctcccctgt cgttcacgta
      481 ctccatatgc cggttgccct tgctgtcgaa ttcgtgaaaa agggtgtcgc tcaggccgtc
      541 gaattcgagc ggcttcacac cgaagcgctc gcagagcgag atgttgaagg tcggggtcgc
      601 cttgacccag cgaccgttca ggaaaagctc cgtgtagccg tggtagaaga agacatccgt
      661 gttcatcaga ctcctgaggc gctccgtggt caggtgattc cggacatcgg caaaaccgag
      721 acgtgaaggg acggcgacag cccttgcggc agcggccagg acgatagcct tcgccacaca
      781 gtacccctcg cgcctcgcga gaatgacgct cgctttgaga cccctgcggg tcaggtcgat
      841 gtcatagggg ttgtaacgta ttccgtctct caccgcatag aagagtctta cacccttttc
      901 acggtcgtcc tttgcgttgc cgacggcctg cctcgcgaac tcgacgacgg agggttcatc
      961 gctgtcgaga aaccaggtcg gcctgaggta tttctcgaag tcttcttcgg gtatagtctt
     1021 ggtcatcgtg ctcatccttg ccggtacaga tagaattttt tgcttcaaac tcaagcgagc
     1081 ggttttccgc cgttcaacct ttcataaacc gcgatgtaag aattgaccat gctgtcgaga
     1141 ctgtatttct cgcgggcctc tttcatgatc ctccgtatct ggggttcccg gacctccgct
     1201 ggcctgcggt ggaactgaac gctcttctcc atgccgtacc agagcccccc cgaatcgtaa
     1261 tctctgaaca gaaacccgtt tcccacgtcc tgggggaatc cgtccgtcgc caacctcaac
     1321 tcgcggattt tatcatggta gccgcctgtg tcacggttcg tcgccgttgc gccgaagagg
     1381 ttcccgatct ggtcgatctg tccgcaaggc tcgtagaggg agcagccgaa gacatcgctc
     1441 gccgccgcgt agccgagcat ggagagttct tcagagaaag gatagtatac gatcttgccc
     1501 cccgacgccc aggcgatccg ccccaggatc tcttcatggg tacggtcgtt tccgacgccg
     1561 ctccccacga tggcgatctg cacgtcgccg tgctcgatgc agaacttaaa ggcgatgtct
     1621 tcgagtatct cgacgccctt ctgagacggg tccagccggg aaggccagta gaacaggatg
     1681 gcgtcggggt tgacaatgag tcccgtccgc ttctggaact ccacgcggtt tatcctcttc
     1741 gcctcgaaga tgtcgtcgtc ggggccgtag ttcctgacac aataaccgca tcgctccggg
     1801 tacattgcga gcgacggtgc gttcatgatg gaaagggcgg caccggcgta atatttttcc
     1861 ttcacctcct tgcggacact gtgtgggacg atcggcctgt ccataaaata atcctccacg
     1921 acctcttgga ggaacctgtg gccgacaaag ttgatcaagg ttgcgctctt gatcgccgtc
     1981 gcctggcagt cgacacagat ctttccatac tcctcggaga agtaaaggtg ttcggtgaaa
     2041 tggtcaagtt ctacccccat gagcatctca agagggacat ggccggtaaa gacattgtga
     2101 accgtgtgga gaatgggaat gcctgcatat tttgcgtggg ccgtgacgac gccgcccgcc
     2161 atccagtcgt ggctgtggac gatcagcttc ccctcgcttt tggcgcttac ctctttgatg
     2221 atttgattcg tgagttctct ctggaactct gcggccgtca gcaaagtatt cccggcgtaa
     2281 gcgctcagga ggttggcgaa gacggcggag ctcacgaggt gtatcttgtc agggtctatc
     2341 ttgtaacgga tctcacgcca ctggctttcg ctcagttggg cctcccgttg gaaccttttt
     2401 ttcaggttta gcgtcgccag atgcacctct atcccgcgat cgatgagtcc ttcacagagg
     2461 gctgatatga cttctccctg cccgccgctc ttccccgaaa tgtattttgc cagcaccccc
     2521 atgtcctccg gcaaccttcc agtttccggt gtgacgatga gcacggccgt cttttccttt
     2581 ttcttcagag ttggaaattg ccgcaaggca acctgcaaca tatcgatttt cctcgtgaga
     2641 acgtgagtgg cctgtgttat cgagcccccg taagggttga aataggaatg aacggcgtaa
     2701 tcctccccga cccgctcatg gagaaaatag atgtggtcgg aggtcgtaag atgacgccat
     2761 ttccgcaaaa tggccccgcc ggcctttttg acttccttct ccattccctc gatgtctttg
     2821 aatatgtcgt actgggtcat cgttcccaac cacccgaagg tgtcccggcc cgcgtcggcc
     2881 caggatgaag tggaaagtcc gtggatgtcg gcaaccgggc attcggcgtt cctgaaccgc
     2941 tctgcaactt ccgttgggtt tgcggggatg acgttgggat gcttcgcgag cgcctcgggg
     3001 agccgtctcc agaactcaaa aattcccttg tcctcccaga tgtgctctcc gatatgttcg
     3061 aagtcgtaac ccagaagaac ggcctcgccg tcgatgagcg ctatgtttcg ggcatattcg
     3121 tccgccgtga tcggatggtg ggggaaacgg taggcgacgt cgtcgctcag ttccctgttt
     3181 ctcggtatga ctatgagctg tgtgcccttg gcacggaaca cggcgttagg tgagatcggg
     3241 cggtgccccg agagaaacat atcgtccctc ttttcgcaaa gcattgcctt gtaacccata
     3301 tccgccacga cactggcaat ctcgttattg tacatcagct cggtattccg gaaggacgcc
     3361 ggaaggacac cgaagagccg cttcatgatc tcccggtgca tgacaacctg ctccctgaat
     3421 tccctctttt cagggtcggc gaaaaggctt gccagcgagt ggtaatacgt ctcctcgagg
     3481 aattccacct ggtgtcgcgc ctctcctgcc tcgcggagat cccgaagggc cttgatgacc
     3541 tcaggccggt agagttccgc ctgctcgagg aaggtccccg acatgctgag ggttatcttg
     3601 aaatcggggt attgtttgat gagttcgatg aaaagacgcg tcgccggaag atagcatttc
     3661 tcggatacct tgaggaagac gtccttgttc aattgatccc agaggaactc ctccctgtcc
     3721 ggatgaagcc ggaagggctg gtgcagctgg aaataaagcg tcaaaagagg catgatggaa
     3781 tatcctggtt tggagactga agtaatgcgg cggtgaggta ggtgatgcct gcccgccgcg
     3841 acaatgtcat taataatttc atattaggga acataagcgg tggtgtcaac gggaaaagtc
     3901 gggaaagtct ggaaatgtcg tgaaagtcgg tcacgggaga attaggtcac cgagccttac
     3961 atggccggcc aggctgccgg ttaaaaccga gcggggatat tttgaccagg ccttgccgac
     4021 cgcttccatc aacacgcggg tgtcacgatt aacaaacaag gtgcacaatc tcaataattt
     4081 tgctatcctc tcccaagttt gcgatgttct ttctgtttgg agttttgcgg tgccgacact
     4141 gaactttaaa ctggaaatta cgagcatatt ccatcatgtg tggccgcttc gttatggtta
     4201 gtccctccta tgtgatctct gaggtgttct gcctcaagga ggaaccacag gacccacttc
     4261 caagttacaa catctcgccg gggcagtccg tttatgccgt cgttcgcgac gaacgggacg
     4321 aaacgaaccg cctggcccga ttccgctggg ggctcgtccc gtcctgggca aaggaccctt
     4381 ccgtcggaaa ccggatgatc aacgcgaggg ctgagtccgt cgccgaaagg ccgagcttca
     4441 aagaggcctt cagggacagg cggtgcctgg tcgtggcgga cggcttctac gagtggcaaa
     4501 aaaccggcag ggcaaaggtc cctttcttca tccgcctgaa atcggggcgg cccttcggca
     4561 tggccggcct ttacgagacc tgggtgtcac cggccgggaa aatactcagg acctgcacca
     4621 taatcacgac ccgtgccaat gacctcttat ctccgattca tgaccggatg ccggtcatcg
     4681 tccataggga gaagcaggcg ctctggctgg accggtcagt tcgtgacaaa aagcatctca
     4741 tcgacatcct gaagcccttt cccccggaag aaatggacgc ctaccgagtt tccggggccg
     4801 tcaattcacc gctcaatgat aaccctgctc ttatcctccc tatcccggat tcctgatcat
     4861 tccggcgatt tgaaccggac aaaaatgggg ttggagacga tggcgccgtc gccgtgcatg
     4921 tccaagcggt agtatacctt ttcccccggc tgaaaacaat cgtcctggaa atcgagactt
     4981 acgggtaacg gggattctac agctttgata acctgacccg aacggatgac cctgaccttt
     5041 acgggcctgg aggcttttcc ctcgagagat atggaaacct ttaccgtcgg tttctcggtc
     5101 aagaggattt cctggccgga aatggctgtc gacctgccgt caagagacga gaccgaaaat
     5161 tcgaggagtt tcgggacatg ggggaaatta ccccgagagg cgtacatcct gccgctcttc
     5221 atggcatcca tgacctcttt cttggtaaat tctttgagta aaaacacggt ctgataattc
     5281 ccgagcttct cgcccgattc gccttctctg tgataatccg aggttgcgat gccccaggga
     5341 ggatgctccc tgaaccctga acagtactcc ttcaatgcca ggtcccagat gtttcccggc
     5401 tccgtgaggg tgatgttgtc gccatagagg gcggaaaatc ccgtatagcc ctttgtctcc
     5461 gtgagaacct gggggtaggg cggggtattg accatgatgg gccccagctt ccttatgccg
     5521 gatttcgttt ccggataatt ccagaaagtc aagccgctgc gggcggttac ataatcgatg
     5581 acgagctggt atggggccat accctggtcc ccgtggtaag ggtcataggg ggaggagtcc
     5641 gtggagagca gcctgtcggc taaaaggacg gcagaaagtc cgagcaggat acccgcaacc
     5701 cgaaacatcc ctttccgacg gaccatcagc aatgaaagga caagcgccgc gacgagcaaa
     5761 acaaggacgg ttgcgtaccc cgcttttccg ccgttatgca gaatcggcag gttttcataa
     5821 tcgggggcct tgctcagacc gacggtcaaa aggcgcctct cgggattgtt tgccgtaagg
     5881 ttcccgttga ggagactgcc cgaccagtaa taaaagggcg tcgtctccga accggggatt
     5941 atgaccatat cgggatgttt cttttcgacc tcctcgatag ccttcagata atggtcggcg
     6001 cccttaaggt ttatggaatt gtgttcgatc ctatacttta agatgtgcct gaggggcggc
     6061 aggccgtatt ccatcgccac gcggtcgtgg tcgttgatca cgagaatacg gaggcccttc
     6121 tgcattgcga ggtcggcaag ctcttgcggg gtgtaggccc cgtcgctgaa ggtcgtgcgg
     6181 gtgtcgatca cgccggggac agcgtagtat tctgctgagg catgctttac cgggagaagc
     6241 atgaagacaa tggcaaaaac gatgatttga tgtcgcaagg caggcatgag ggtaagcatg
     6301 gctgaagttc tcggactatg agtcataaga ggccctgaaa tgaaccgatg acgcgggggt
     6361 tgctaacctt aaatcgtttc cagggggcga gtcaagtttt tcccaagtcg ggggaatttt
     6421 tggtaattga ttgaattttg gcgtctgtgg tataaattta tgaagaaata gccgataaat
     6481 tgaaggaatt ggcgaaggag cttagaagaa aatataaagt gagtgaaata attgtgtttg
     6541 gctcctacgc caggggggac ttaaatgaag gaagcgatat cgacctactg atagtagggg
     6601 atttcaaaga gaagtttcat aaaagaattg cgagcatttt gggtttgacg gatctgccga
     6661 tagaagcttt gtgttacaca gaagaggagt ttagagagat gatcacgaat aacagaggct
     6721 tcatatcaga ggtattgaag gagggtgtgg cgatatgaag acgctttttt agaaaggaaa
     6781 attttttggt cagatgagta attccaggag ctacgaaatc gtcattgaac caaagggtac
     6841 gtggaaggcc cttcaatggg gagaactctg gcaataccgg gagctcctcc gtttcctcac
     6901 ggttcgggac atcaaggtgc gttacaaaca gacggttctg ggcgggttgt gggccatcct
     6961 gcagcctttc atgagcatgg tggtatttac cgtcttcttc gggatgctcg cgaaagtccc
     7021 ctcggacggc cttccttacc ccattttcgt gtataccgcg ctcctgccct ggcagttttt
     7081 cagcaacggc atcagcaatg cgggtaacag cctcgttgcg agcagcaat
//
LOCUS       DAOTMX010000044         7100 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_9220_length_7100_cov_3.667282, whole genome shotgun sequence.
ACCESSION   DAOTMX010000044 DAOTMX010000000
VERSION     DAOTMX010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 7100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7100
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9220_length_7100_cov_3.667282"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="P5573_02150"
     CDS             complement(<1..342)
                     /locus_tag="P5573_02150"
                     /inference="COORDINATES: protein motif:HMM:NF016494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxD N-terminal domain-containing protein"
                     /protein_id="HRT26899.1"
                     /translation="MEKSVSEIAEILNGTVIGDGRLTIRDVKGIDEAGEGDLTFIANQ
                     KYRKHLEDTGASAVLVSPDVKQSKKTLIVVRDPYASMARVLSVFYPPDREVAGISPLA
                     FVAKDAFVSEDA"
     gene            complement(355..921)
                     /locus_tag="P5573_02155"
     CDS             complement(355..921)
                     /locus_tag="P5573_02155"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HRT26900.1"
                     /translation="MKRFYLSAVGLVILLSLLVPATVRAEKIGFVDIREVIARSEAGK
                     KAQAEFKKAVEKKSQLVKQKEADLKKDKSEYDKVRPTLSDTAAKEREMELQKKYRDYQ
                     RLVNDAGEAMKRKDQELSRRLLPEIYKVITAVGARGGYTAILDISNPVVVYSSGENNL
                     TDQVISEFNKVLIKAQGKKKTSEKGKTK"
     gene            complement(1024..3612)
                     /gene="bamA"
                     /locus_tag="P5573_02160"
     CDS             complement(1024..3612)
                     /gene="bamA"
                     /locus_tag="P5573_02160"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HRT26901.1"
                     /translation="MPAAHGDELKKVALFPFDVYAEKDRASLQKQVGESIQNNLLRSR
                     AIQIIPQDVLMKSLNGRQIDEKTALQVGREVGADVAIIGSVSQLGKVVSIDAKLIDVK
                     SGQAPQGVFVQGTLAEGIGNLSARLTDDLILKIAAEKRIASIVVMGNRKIETSAIMNV
                     LKESPGKLFSPADLSADIKAIYKTGYFSDVKADVKDTPGGKVITFTVQERPMISNVVV
                     KGNDAIETKEIEAVLTVRLRQFLDVDKIKEDVEKIRTLYHNKGYFNVEIKYDIEMKSE
                     KEAILTYTIVEGRRLYIKSISFTGVQAFSPKELKNMMDTSEYGLFHFFTDSGILKEDK
                     LKEDVKKLNAFYLNNGYINAKVGDPEITHDKRWIYITIPVIEGKRFKVGNVTISGDTI
                     SVPREKLLSELQIKKKEFYDREAIMRDIDYLTQAYNDEGYAYADVTPITVPDEKEQKV
                     NIEYQVVKGEKVHFNRISIAGNTKTRDKVIRRQLAVVEGDLFSRQKMKESYANLAQLR
                     YFEEVDIQTEKGPDKNLMDVNIRVKEKPTGMVSFGAGYSALDSMVMSGQIVQRNLFGK
                     GQSLGLTAAIGGRSRNYDLYFIEPWLFDMPLWSKTNLWNWERSYDTFNVESFGFGQML
                     GYTIFPKWRTTGYLGYRLSNDKIKDVLPTAAHIIKTQEGTTLLSGVEAMLVRDTTNDN
                     LFPTKGSKNAVSVEYTGGLMQGDVDYTKYQASSAWFIPLPGETVFSARGRIGYIQERG
                     DKEIPVFYRFWLGGIDSLRGLRDVGPRDPETGDFIGGLTFLNFNVEYIFNLVKNAGMK
                     GVVFFDTGNTWNSGYHFDDMRMTAGGGIRWFSPIGPIRIEYGFVLDRKTGEPTGRWEF
                     TIGTSM"
     gene            complement(3672..4340)
                     /locus_tag="P5573_02165"
     CDS             complement(3672..4340)
                     /locus_tag="P5573_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRT26902.1"
                     /translation="MIKVSKLNKTFIKDGKRIEVLKDLDLDVQRGESLSIVGASGAGK
                     STLIHILGTLDHPTSGEVIIGGSDVFRWDGGKLAAFRNRMIGFVFQFHHLLPEFSSLE
                     NTMMPALIGGMAGRKAKEKAETVLKEVGLGDRLAHKPGELSGGEQQRVAVARALVMEP
                     EIILADEPTGNLDTETGRRIEDMLISLNRERGITLVVVTHNASLAQRMSRSIGLQDGK
                     IRNQ"
     gene            complement(4352..5599)
                     /locus_tag="P5573_02170"
     CDS             complement(4352..5599)
                     /locus_tag="P5573_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HRT26903.1"
                     /translation="MGYELFISLRYLRAKRKQIFLSIITVISICGIFLGVAALIIVLA
                     VMNGFEKELRDKILGMNSHVVVMGYTGPVKDFERVREKVDSVKGVVASTPFIYSQVML
                     KSGSHVSGVVLRGLEPEDALKVINVGKMVEGSLSRLSERGRTGPGIVVGKELAKNLGL
                     SLGDPVDIVAPMGITTPMGMVPKMRRFEVVGIFDSGFYEYDSSLACISLKESQDFLGM
                     QGMVTGLEVKVDDIFRAKEIARAIELELGFPFWVRDWMQMNKNLFSALKLEKRVMFII
                     LTLIVLVAAFNIVTALIMLVMEKTRDIAILKSMGATSKSIMKIFIFQGVVIGTIGTIL
                     GCAGGLAVALNLERISLFVEKTFGFKILPGDVYYLNALPSQVNYGDVAVIVVGTMVIS
                     FLATIYPSRRAARLDPAEALRYE"
     gene            complement(5611..>7100)
                     /gene="lysS"
                     /locus_tag="P5573_02175"
     CDS             complement(5611..>7100)
                     /gene="lysS"
                     /locus_tag="P5573_02175"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HRT26904.1"
                     /translation="EKIESLKAEGIELYPNDVKVSDTAAGILARLGGLDNDALSRISD
                     RFSLAGRMMAIRDFGKGAFVSLQDRTGRIQVFLRKDRLGEKTFSLFKKLDVGDIVYVA
                     GKAFRTRTNELTIDADEFRLLSKAVRALPEKWHGLTDTEVRYRQRYLDLIVNPKVADV
                     FKKRSRIIEIIRQFMKERDFLEVETPMMQYMAGGAAARPFKTYHNTLDMEFYLRIAPE
                     LYLKRLLVGGMERVYEINRNFRNEGVSSSHNPEFTMMEFYLAYATYEDLMVMTEELIT
                     RICLELFGSLKITYQGQELDFSRPWRRFTVKEALVEFGGVEPTVLEDRNKAVAYAKSL
                     KLDVDDMPLGKVIMALFDETVEPNLVQPTFITGYPVEVSPLARRNASDPSLVDRFELY
                     IAGKEIANAFSELNDPVDQRERFLMQLKEREAGDEEAHGMDYDYIRALEYGMPPAAGE
                     GIGIDRLVMILTDSASIRDVILFPHMRVKDEARKEEDDEPGVIEG"
BASE COUNT         1382 a         2086 c         1843 g         1789 t
ORIGIN      
        1 cgcatcctcc gagacgaacg cgtcctttgc tacgaacgca agtggactta ttccggccac
       61 ttccctatcg ggggggtaga agaccgagag gaccctcgcc atagaagcat agggatccct
      121 cacgacgatc agcgttttct ttgattgctt gacatccggc gagacgagaa cggcggaggc
      181 gcctgtatcc tccagatgtt tgcggtactt ctggttggcg atgaacgtca aatcgccctc
      241 ccccgcttcg tctatcccct tgacgtcgcg gatcgtcaac cttccgtccc cgatgaccgt
      301 cccgttcagg atttccgcta tctcagagac acttttttcc atgggacgca acctttactt
      361 tgtcttgccc ttctcagagg ttttcttctt tccctgggcc ttgatgagga ccttgttgaa
      421 ttcgctgatc acctggtcgg tcaagttatt ctctcccgag gagtaaacca cgacgggatt
      481 gctgatgtcg aggatcgcgg tatagcctcc cctggcaccg acggcggtta tgaccttgta
      541 gatttcgggg agcagcctac gggaaagttc ttggtctttg cgcttcatcg cttcgccggc
      601 gtcgttaacg aggcgttgat aatcgcggta tttcttctgc agctccattt ctctttcttt
      661 cgccgcggta tccgaaagag tgggtcgcac tttgtcgtac tcgcttttat ctttcttgag
      721 gtcggcttcc ttctgcttga caagctgact ctttttctct acggctttct tgaactccgc
      781 ctgcgccttc tttcccgcct cagaacgggc gattacctcc cggatgtcga cgaaaccgat
      841 tttttcggcc ctgacagtcg ccggtacgag gagagacagc aagatcacaa gccctactgc
      901 cgatagataa aaccttttca tgatgatccg tcctttaatt tgtcagggtg gagagaggac
      961 aacagcctcg ccttcaaccc cgtattttcc ttttacccga tcccgggagc cccacggtcg
     1021 cccttacata gaagttccga tcgtgaattc ccacctgccc gtgggttcgc ccgtcttcct
     1081 gtcgaggacg aagccgtatt cgatgcggat gggcccgatc ggagaaaacc agcggatacc
     1141 gccgcccgcc gtcatcctca tgtcgtcgaa atgataaccg ctgttccacg tgttgcctgt
     1201 gtcgaagaag acaacgcctt tcatccccgc gttcttgacg agattgaaga tgtattcgac
     1261 gttgaagttc aagaaggtca accctccgat gaagtcgccg gtctccggat cacgcggtcc
     1321 gacgtcgcgg agccccctga gcgaatcgat acctcccagc cagaagcggt aaaagaccgg
     1381 tatttctttg tccccgcgct cctgtatgta cccgatccga ccgcgggcgc tgaagacagt
     1441 ctcaccgggg agcgggataa accaggcgga acttgcctgg tatttagtgt agtccacgtc
     1501 cccctgcata agaccgccag tgtactcgac ggaaaccgca tttttcgagc ccttcgtcgg
     1561 gaacagattg tcattcgtcg tatccctaac aagcatggcc tcgacgccgc tcaacagggt
     1621 cgtgccctcc tgcgttttga tgatgtgtgc cgccgtcggc agaacgtcct ttatcttgtc
     1681 gttcgacagc cggtatccta agtagcctgt tgtacgccac ttgggaaata tagtgtaacc
     1741 taacatctgg ccgaaaccga atgactccac gttgaaagta tcgtaacttc gctcccaatt
     1801 ccaaaggttg gttttgctcc acagaggcat gtcgaacaac cagggctcga tgaagtagag
     1861 gtcgtagttt ctcgacctgc cgcctatcgc cgccgtgagt cccaggctct ggcccttgcc
     1921 gaaaagattg cgctggacga tctgacccga catgaccatg gaatcgaggg cgctgtaccc
     1981 ggccccaaag ctcaccatcc ccgtgggttt ttctttgaca cggatgttca cgtccatgag
     2041 gttcttgtcg ggtccttttt ccgtctggat gtccacttcc tcgaagtacc gcaactgggc
     2101 aaggttcgcg taactctctt tcatcttctg ccgactgaat aggtcgcctt ccactaccgc
     2161 cagttgccga cggatgacct tgtcccgcgt cttggtgttt cccgctatgg aaatgcggtt
     2221 gaaatggacc ttttccccct tgaccacttg atattctatg ttgactttct gctctttctc
     2281 gtccgggacg gtaatgggcg tgacgtcggc ataggcataa ccctcgtcgt tgtacgcctg
     2341 ggtcaggtaa tcgatatccc tcatgatcgc ttcacgatca tagaattctt tcttcttgat
     2401 ctggagctcc gacagaagtt tctcccgggg aacggagatt gtatcccccg agatggtcac
     2461 attacctacc ttgaaccgtt tcccttctat gaccgggatc gtgatgtaga tccatcgctt
     2521 gtcgtgggtg atttccgggt ccccgacctt agcgttgatg taaccgttat tcaggtaaaa
     2581 ggcgttcaac ttcttcacat cttctttgag tttgtcttct ttcaggattc ccgagtcggt
     2641 gaagaaatgg aaaaggccgt attcgctggt atccatcata ttcttcagct ctttggggct
     2701 gaaggcctgc acacccgtga aggagatgct ctttatgtac agcctgcgtc cttcgacgat
     2761 cgtataagtc aggatggcct ctttctccga cttcatttct atgtcgtatt tgatctcgac
     2821 gttgaagtag cccttgttgt ggtaaagggt cctgattttc tccacgtcct ctttgatctt
     2881 gtctacatcc aggaactgcc gcagcctgac ggtgagcacg gcctcgattt ctttggtctc
     2941 tatagcatcg tttcccttta cgaccacatt ggagatcatg ggtctttcct ggaccgtgaa
     3001 cgtgatcacc ttccctcccg gggtgtcttt gacgtccgcc tttacgtcgc tgaagtagcc
     3061 tgtcttgtag atggccttga tgtcggcgga gaggtcggcg ggggaaaaga gtttccccgg
     3121 ggattccttg agaacgttca tgatggcact cgtttcgatc ttgcggtttc ccatgacgac
     3181 gatcgaggcg atgcgctttt ccgcggcgat cttcaggatg aggtcgtctg tcagtcgggc
     3241 cgagaggttg ccgatgcctt ccgccaaggt gccctgaacg aacaccccct ggggtgcctg
     3301 gccggacttt acgtcgatca acttggcgtc tatgctgacg accttcccga gttgcgacac
     3361 gcttccgatg atcgcgacgt cggccccgac ctcccgtccg acctgcaggg ccgttttttc
     3421 gtcaatctgc cttccgttca gcgacttcat gagtacatcc tgggggatta tctggatcgc
     3481 cctacttctg agcaggttgt tctggatcga ctcacccacc tgcttctgca aggaggcgcg
     3541 atctttttcg gcatacacat cgaaggggaa gagggcgacc tttttcagct catccccatg
     3601 agctgccggc atagagcaaa taaggaggac cgaaaagaaa caaatcgtcg cggtgaggaa
     3661 gccgttcttt ttcactggtt tcgaatcttt ccgtcctgca gcccgatgct gcgtgacata
     3721 cgttgagcta aggatgcgtt atgagttacc acaaccagtg tgatccctct ttccctgttc
     3781 agggagatca acatatcctc tattcttctc cccgtttccg tgtcgaggtt ccccgtgggc
     3841 tcatcggcca ggatgatctc cggttccatg acaagcgccc tagccaccgc taccctctgc
     3901 tgctcgcccc cagacagttc cccgggtttg tgcgccaacc tgtcgcccag acctacttct
     3961 ttgagtactg tttctgcttt ttccttcgct tttcggccgg ccataccgcc tatcaaggcc
     4021 ggcatcattg tgttctccag actgctgaac tccgggagca ggtgatgaaa ctggaaaacg
     4081 aagcctatca tcctgtttcg gaaggccgcc aactttccgc cgtcccatcg gaaaacatcg
     4141 ctccccccga ttatcacctc tcccgatgta ggatggtcca gtgtccccag gatatggatg
     4201 agggtgctct tgccggcccc tgaggcaccg acgatggaca gggactctcc cctttgtaca
     4261 tccagatcta ggtctttgag gacctctatc ctcttgccgt ctttgatgaa tgtcttgttc
     4321 agcttgctga ctttgatcac gacgagcttt cttattcgta tctcagcgcc tcggccgggt
     4381 cgagccgggc tgcccgcctg gaggggtaga tcgttgccag gaagctgatc accatcgttc
     4441 caacgacgat cacggcgacg tctccgtagt tcacctgcga gggcaaggca ttgagataat
     4501 agacgtcccc gggaaggatc ttgaacccga aggtcttctc gacgaacagg gatattctct
     4561 ccagattaag ggcaacggcc agtccgccgg cgcaacccaa gatcgtcccg atggtgccga
     4621 taacaacccc ctgaaagatg aatatcttca tgatgctttt ggacgtcgct cccatggatt
     4681 tcaggatagc gatgtccctc gtcttctcca tgacgagcat gatcagcgcg gtgactatgt
     4741 tgaaggcggc gacgaggacg atcaacgtca agatgatgaa catcacccgt ttctcgagtt
     4801 tgagcgccga gaaaaggttc ttgttcatct gcatccagtc ccgcacccag aagggaaagc
     4861 cgagctcgag ctcgatcgcc cgggcaatct ccttagccct gaagatgtcg tccaccttga
     4921 cttccaaccc tgtcaccatg ccttgcatcc cgagaaagtc ctgactttcc ttgagggaga
     4981 tgcaggcaag ggaagagtcg tactcgtaaa agcccgaatc gaagataccg actacctcga
     5041 aacgtctcat cttgggtacc atgcccatgg gtgtcgtgat ccccatcggg gcgacgatgt
     5101 ccacagggtc accgagtgaa agcccgaggt tttttgccaa ctccttcccg acgacgatac
     5161 cgggtccggt ccgccctctc tccgaaaggc ggctgaggct gccttcgacc atcttcccca
     5221 cgtttatcac cttgagggcg tcctccggtt ccagtcccct cagcaccaca ccgctgacat
     5281 ggcttccgct cttcagcatc acctggctgt agatgaaagg cgtggaggcg accacccctt
     5341 tgaccgaatc gaccttttcc ctgacccttt cgaagtcctt gaccggcccg gtgtacccca
     5401 tcacgacgac gtgggagttc atgccgagga tcttgtcccg cagctccttc tcgaagccgt
     5461 tcatgacggc gagcacgatg atcagcgccg ccacccccag gaatataccg caaatcgaga
     5521 tgacggtgat gatcgagaga aatatctgct tccttttggc gcggagatac ctgaggctga
     5581 tgaatagttc gtatcccata cggcttccgg tcaaccctcg atcactccgg gttcgtcgtc
     5641 ttcctccttt cttgcctcgt ccttaaccct catgtggggg aagaggatca cgtctctgat
     5701 cgaggccgag tcggtcagga tcatcaccag ccggtcgatc ccgattccct ctcccgccgc
     5761 gggcggcatc ccgtactcga gggcgcggat gtaatcgtag tccatcccat gggcctcttc
     5821 atcccccgcc tcgcgctctt tcagttgcat cagaaaacgt tcccgctgat ctacggggtc
     5881 gttgagttca gagaaggcgt tggcgatctc cttcccggcg atgtaaagct cgaagcggtc
     5941 gacgagggaa gggtccgagg cgtttctcct cgccagggga gaaacctcta cggggtaacc
     6001 ggtgatgaag gtcggctgca cgaggttggg ttcgacggtt tcgtcgaaaa gggccatgat
     6061 gactttcccg aggggcatgt catcgacgtc cagtttcagg ctcttagcgt aagccacggc
     6121 cttgttccgg tcttcgagga cggtaggctc gacgccgccg aactccacga gggcctcctt
     6181 gactgtaaac cgtctccagg gacggctgaa gtccagctcc tgcccctggt atgtgatctt
     6241 gagggaaccg aaaagctcaa ggcagatgcg cgttatcaac tcttccgtca taaccatgag
     6301 gtcttcgtaa gtggcgtagg ccaggtagaa ttccatcatc gtgaactcgg ggttatggga
     6361 ggaagaaacc ccttcattgc ggaaattgcg gttgatttcg taaacccgct ccatcccgcc
     6421 gacaagaaga cgtttcaggt aaagttccgg ggctatccgc agataaaact ccatgtcgag
     6481 ggtgttgtgg taggtcttga aaggacgggc ggccgcccca ccggccatgt actgcatcat
     6541 aggcgtctct acttcaagaa aatcgcgctc tttcatgaac tggcggatta tctcgatgat
     6601 gcggcttcgc ttcttgaaga cgtcggcaac cttcgggttg actatcaggt cgagataacg
     6661 ctgccggtat ctcacctccg tgtccgtcag tccatgccac ttctcgggca acgcccttac
     6721 cgccttggaa agaagcctga actcatccgc gtcgatcgtc aactcgtttg tcctggtccg
     6781 gaaggcctta ccggcgacgt agacgatgtc cccgacatcc agtttcttga agagggaaaa
     6841 ggtcttttcg ccgaggcggt ccttccgcaa gaagacctgt atccggcctg tccggtcctg
     6901 gaggctgaca aatgccccct ttccgaagtc tctgatcgcc atcatacggc cggcaagcga
     6961 gaaacggtcg gatattctgc tcagggcgtc gttgtccagg ccgccgagtc tggccaggat
     7021 accggcggcg gtatcggaaa ccttgacgtc gttcgggtag agctcgatgc cctcggcctt
     7081 gaggctctct atcttttccc
//
LOCUS       DAOTMX010000045         7030 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_9318_length_7030_cov_4.529462, whole genome shotgun sequence.
ACCESSION   DAOTMX010000045 DAOTMX010000000
VERSION     DAOTMX010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 7030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7030
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9318_length_7030_cov_4.529462"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1081)
                     /locus_tag="P5573_02180"
     CDS             complement(<1..1081)
                     /locus_tag="P5573_02180"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HRT26905.1"
                     /translation="MKMQGLRAKLMVWGIILSVIPVVILGLMTYFWTARSMEEEVNNK
                     TLMQAKSSAQMVDAIISAEAVNMAIHARSKEVLEAVKEANGGAPGEKAARLTNTMVQW
                     QAVAGDRYELVVVADRNGVIFADSGGGGAKGVNMADREYFKLAMQGKPSLDNVVMNKK
                     TGNPTNVVSQPVVGEDGSAIGAIAATLKIDPIAQKLNEIKLGKTGYVYAMNKQGLFIM
                     HPDSKRILKTNLATEKGMEAFAQISLSGKEGVGEYTLDGVRKLAGFIPVKSTGWVVVA
                     AVNKNEVMSIAYTIRNMILGCIIIVVLAAGCFAFFGARAIAKPIQVAVEKTTASSEQI
                     ASASAQVASASQALAEGTSEQAAAVE"
     gene            complement(1595..1726)
                     /locus_tag="P5573_02185"
     CDS             complement(1595..1726)
                     /locus_tag="P5573_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT26906.1"
                     /translation="MPVAASTDAFKARFTQIIRNGIDAIFRQMAFNIFRRGKVLGTV"
     gene            1839..2234
                     /locus_tag="P5573_02190"
     CDS             1839..2234
                     /locus_tag="P5573_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2779 family protein"
                     /protein_id="HRT26907.1"
                     /translation="MLDKFKIPVVALFMATYLVFFVSFPAMAGLISSMPSSIEMAGKG
                     KEAEIDKVKKVLEMQIVADKLQAYGLSVEEVKAKLSSMSDEQIQVVAQASDRVLAGGD
                     GAGFVIAVLVIIILVIVILKLLDKRIIIK"
     gene            2244..2735
                     /locus_tag="P5573_02195"
     CDS             2244..2735
                     /locus_tag="P5573_02195"
                     /inference="COORDINATES: protein motif:HMM:NF015377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HRT26908.1"
                     /translation="MNILIIFFLVLAEGAGTTYIIDGMPFVRQETQYCGPAALSSVLS
                     YYGDNVDQKTIGKEVYSPGLKGALITDLENYGRKRGFQTKIGRASIADIKEILLNNKP
                     VIVLVDLGFWVVSRMHYLVVYGYSDRGFLVHDGLKPAQEYPYEEFEKIWQKTGNSFLL
                     VYR"
     gene            2789..3175
                     /locus_tag="P5573_02200"
     CDS             2789..3175
                     /locus_tag="P5573_02200"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRT26909.1"
                     /translation="MIEDSLTAEQHNDLGYVYEQKKAYELAEKEYLLAVKKREDWPIP
                     YFNLGNLACKTGNYKKSEDFFRKVLTLDPNNTDAMNNLAVALLMQDRLQEAKLLVEKA
                     LKTRHKDEYEDTLKEIQKKEVLSHRR"
     gene            complement(3508..3762)
                     /locus_tag="P5573_02205"
     CDS             complement(3508..3762)
                     /locus_tag="P5573_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT26910.1"
                     /translation="MAVEHFARARAATLFGLPASADIAPAFAVSIPVSALVFEVYNPG
                     HSRLFSSPNSDSLAKSSSFFEVHGVEHIGGSKDGHANYGT"
     gene            3968..4408
                     /locus_tag="P5573_02210"
     CDS             3968..4408
                     /locus_tag="P5573_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT26911.1"
                     /translation="MKALRMAVMVFMLFLFASPAVAGDFDWMNDFNAQASLDPSGFRA
                     RLAARFNIGDAQISAVMSNIHDPAGVYMAFRLGEMSHQPVERVVDVYKKNRGKGWGVI
                     AKSLGIKPGSPEFHALKRGDDLYTHKEKGKGKGGKGKGKGKEKG"
     gene            4549..6936
                     /locus_tag="P5573_02215"
     CDS             4549..6936
                     /locus_tag="P5573_02215"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HRT26912.1"
                     /translation="MSVKKNLQKKRDRVKRIRQDRARQSQLIDAQWNLDLAVDALRLR
                     DYEKAGLHVEKALHLKPDWHHALRLKSMIALEIPKPDIATACSCLEKIVQKDPNDVIT
                     MFSLADCYFRLQRYADEISLLEGFLSRYKDRRSKDIDRYKKQAREWIRESKEYQEAER
                     FRAMLKASDTGTASRAARFPDKKQVPTATAAPSPPETAGPVLPSLDLSPFQEARKFRV
                     EPTIRFRSGDGDLVTDVAAAVPEDLTDYRLRLAYHRLRLLKDFEELLCLDQLQGVTHY
                     WYQVETVASVLKRFRGRVLLADEVGLGKTIEAGMVIKEYLLRGLARKVLILTPPSLVS
                     QWQEEMAVKFGLSFVTPDHEAFQGDSERFWREQDLVIASINTAKSQRHSDVVTNIDYD
                     LVVVDEAHYLRNKKTLNHRLVGALRKRFLLLLSATPVQNNLIELYNLITLLQPGLLST
                     EAAFKRDYVKRGNLRQPNNPEQLRRLLKEVMIRNTRSMVDVKLPRRFATTLYVTPTEE
                     ERETYELLSTLTRLLHEKEGPTELLTIRNLQMAAGSSPFALRDALGHLQARLEGADHP
                     IRHLLRDLDARLARIDDSAKGRQFLALVEQNREKKLIFVRYQRTLAYLAGLLRERGLP
                     FAVFSGTMSTYEKDAAVEAFRREVPILLSTETGGEGRNIQFCNTMVNYDIPWNPMRLE
                     QRIGRIHRIGQERDVFIFNLCLAGSIEDYMMRILDDKIHMFELVIGEIDTILGNLETD
                     RDFSSLILDVWLRSGDEALRRENFARLEDEVIAAKKAYEKTKALDEMLFGDDYEV"
BASE COUNT         1686 a         1865 c         1881 g         1598 t
ORIGIN      
        1 cctccacggc ggccgcctgc tcggatgtgc cttccgctaa tgcttggctc gccgaggcaa
       61 cctgtgcgct cgccgaggcg atctgctcgg aactcgccgt cgtcttctcg accgcaacct
      121 ggatcggctt ggcgattgcc cgggcgccga agaaggcgaa gcaaccggcg gcgaggacga
      181 cgatgatgat acaccccagg atcatgttcc tgatcgtgta ggctatcgac atgacctcgt
      241 ttttattcac ggccgcgacc acgacccacc ccgtgctctt caccgggata aaaccggcca
      301 attttcttac cccgtcgagc gtgtattccc ctaccccttc tttcccggag agggagatct
      361 gggcgaaggc ctccatccct ttctccgtgg ctaaattggt cttcaagatc cgttttgagt
      421 cggggtgcat gatgaacaga ccctgtttgt tcatcgcata cacatacccc gtcttcccca
      481 gctttatctc gttgagcttt tgcgcgatgg ggtcaatctt tagggtggcg gcgatagcgc
      541 cgattgcgct gccgtcctcg ccgacgaccg gctgggagac gacgttggta gggttgcccg
      601 tttttttgtt catcaccacg ttgtccaagc tcggcttccc ctgcatggcc agcttgaagt
      661 actcacgatc ggccatgttt acgcccttgg cccctccccc cccgctgtcg gcgaagatga
      721 cgccgtttct gtccgctacg accactaatt catagcgatc cccggcgacg gcctgccatt
      781 gaaccattgt gttcgtcaga cgggccgcct tttcccccgg cgcaccgccg ttggcctctt
      841 tgacggcctc caacacctcc ttgctccgag catgtatagc catgttgacg gcttctgctg
      901 atatgatggc gtccaccatc tgggccgaac tcttggcctg catgagggtc ttgttgttca
      961 cctcctcttc catgctccgt gcggtccaaa aatacgtcat caaccccaag atgacgacgg
     1021 ggatcaccga cagaattatc ccccacacca tcagttttgc cctcagccct tgcatcttca
     1081 tatttaagta tcctccttga aatcatttac gccttttcgg agccgttttg cgcccggccc
     1141 cggctgttgc ggttgatatt tacatcggca taatggggga gaactttaaa aagacaccgc
     1201 agtgccttcc cggagggtct atctacccct gggaaatctc ttgacaccga ccgacgctaa
     1261 ccgtaaacat ggtttgtcat cggtgttctt tggctcggcc ctacgccccg atttcgacga
     1321 gttagaacca catcaaaccc cgggactttt cactcttgca cgtatggcga agtataaaat
     1381 aatcgagacc tttgaatttc tctaaatttc acgaacgtcc tatttctatc cccgatttgc
     1441 tcaaaaacgg ggatgcgata aagttgccta ttgctggatg acgataaccg tgactgctat
     1501 gtcatcacta ttcacctccc aaatgctgtt ttcacccacc aggcgggagt ccccctgtcc
     1561 atatctacaa ccctggacac acacctcttt ccatctatac tgtccccagt actttacctc
     1621 tacgaaagat attgaaggcc atctgcctga agatggcgtc gataccgttt ctgattatct
     1681 gggtgaaccg cgccttgaag gcatccgtgg atgccgctac aggcatgaaa attttgttgc
     1741 gtttcgacgc acgttatgct tcatttcgat gcgtatttct gttgatagac acttttgaca
     1801 cctcagtatc gataccgcaa aggaggagat ggtcgaatat gttggacaag tttaaaattc
     1861 cggttgtagc gctgtttatg gcaacttatc ttgtcttctt cgtgagtttt ccggccatgg
     1921 ccggcttgat ttcttctatg ccgtcatcaa ttgagatggc cggcaagggc aaagaagcag
     1981 agatcgacaa ggttaagaaa gtcctggaaa tgcaaattgt agccgataaa ctgcaggctt
     2041 acggcctgtc tgtcgaagag gtgaaggcta agctgagcag catgtccgat gagcagatcc
     2101 aggtagtggc ccaggcctcg gaccgggttc tggcaggtgg agacggggct ggatttgtca
     2161 tcgctgtctt ggtaatcata atcctggtca ttgttatttt gaagcttttg gataaaagga
     2221 tcatcataaa gtaaagtaac gcattgaata tccttatcat cttctttctc gtcctggcag
     2281 aaggtgccgg tacaacgtac atcatagatg gtatgccttt tgtcaggcag gaaacgcagt
     2341 attgcggtcc agcagccctc tcttcggtac tatcctacta tggcgataat gttgatcaga
     2401 aaacgatcgg gaaagaggta tattctcccg gattaaaagg tgccctgata acggacctgg
     2461 agaactacgg cagaaaaagg gggtttcaga caaagatagg acgtgcctcc attgccgaca
     2521 tcaaagaaat tctcctcaac aataaacccg tcatcgttct tgttgatttg ggattctggg
     2581 tggtatccag gatgcattac ctggtcgttt atggatattc cgataggggg tttttggtcc
     2641 atgatggtct taaaccggcc caggaatatc cttacgagga atttgagaag atatggcaaa
     2701 aaacaggcaa cagcttctta cttgtttacc ggtagcaatc tggctccttg taatttcact
     2761 gtctgcctgt acctttcccc agatagtgat gatagaggat tcacttactg cggaacaaca
     2821 taatgacctt ggttatgttt acgagcagaa aaaggcttac gaactcgcag agaaggagta
     2881 cctgctggcg gtcaagaaac gcgaagactg gccgataccg tacttcaatc tcgggaatct
     2941 tgcttgcaag acaggaaact acaagaaatc tgaagatttt ttccgtaaag tactgacact
     3001 cgaccctaac aacaccgacg cgatgaacaa tctggcagtt gctttactta tgcaggatcg
     3061 tcttcaagaa gcgaagctgc tggttgaaaa agctcttaag acaaggcaca aggacgagta
     3121 tgaggacaca ttgaaggaga ttcagaaaaa agaagtcctt tctcatagga ggtaacgaaa
     3181 tgagaaaact acttttcgga gcgataattt tggcagcgac gattttttca cccgtcgggg
     3241 caacggcaga tgttgatgtc cacattggaa taagtttgcc gcctcttatc tcgttttcgg
     3301 ggccgccggc tgttatcgta ttacccgaca cccctgatgt ctatgtagtc ccttatatcg
     3361 aagtggacct tttcttctgg ggcggttggt ggtggcgtcc atggggaggg cgatggtatc
     3421 gctctcagta ttatgaccgg gggtgggttt attacggcgg tgttccaaac ttctactttt
     3481 atgtggattc gggatggcgt gattgtttca ggtcccataa ttggcatggc catccttgga
     3541 accaccaata tgttccacac catgaacttc aaagaaactg gaagacttgg caagcgaatc
     3601 gctattggga gaagaaaaaa gtctggaatg tccagggtta taaacctcaa acaccaaagc
     3661 agagacagga attgagacgg caaaggcagg agcaatatcg gcagaagccg gcagtccaaa
     3721 gagagttgca gcacgagcac gggcgaagtg ttcaacagcc atctcagcca cgtcagccac
     3781 aggagcggta tcttgaacca aggggcgagc ggccccaggg acgggattta gagcccaggg
     3841 gggagaaagg tcatggggga tcacagcaac ggcaatctcc ggggagacat gaaggcggag
     3901 acaaaggaca tggaaggtgg taggacaaga agtgattcgt ttatctgtgc gtgaatagga
     3961 ggaaatgatg aaggctctga gaatggcggt tatggttttt atgttgtttc tctttgcatc
     4021 cccggctgtg gcgggggatt ttgactggat gaacgatttc aacgcccagg cctcgctgga
     4081 tccgtctgga ttccgggcaa ggcttgccgc gcgattcaac atcggggacg cgcagatcag
     4141 cgccgtcatg agcaacatac atgatccggc cggtgtatac atggcgttcc ggttagggga
     4201 gatgtcccac cagcctgtag agcgggtggt cgatgtgtac aagaagaaca gagggaaagg
     4261 gtggggtgtc atagctaaga gtctcggaat caaaccgggc tctccggaat tccatgcatt
     4321 gaagcggggc gacgatttat acacgcacaa ggaaaagggg aaaggaaaag gcgggaaagg
     4381 aaaaggaaag ggaaaagaga aaggctgagg ttgattttga cgcagggtcc aggtgccctg
     4441 cgacccccgg gcgactgcga gacaacaaaa cctctttagc cgtccgacgg aataccatcc
     4501 gacaaggagt agcatctacc caggaaaggt cacggtccaa ggaaaggtat gtccgtcaag
     4561 aagaatcttc agaaaaaacg ggatcgggta aagcgaatcc gccaagaccg ggcgagacaa
     4621 tcgcagctga tcgacgcaca gtggaacttg gacctggctg tggacgcttt acgtctacgt
     4681 gactatgaga aggccggact ccacgtcgag aaggcccttc acctgaagcc ggactggcac
     4741 cacgccctac gtctgaaatc catgattgcc cttgaaattc cgaagccgga catcgcgacc
     4801 gcttgctcct gcctggaaaa aatcgttcaa aaagacccca acgacgtcat aacgatgttc
     4861 tccctggccg attgctattt ccggctgcaa cgttacgccg acgaaatatc cctgttggaa
     4921 ggatttttgt cccgttacaa ggaccggcga agcaaggata tcgatcgata caagaaacag
     4981 gcacgagaat ggatccggga gtccaaggaa taccaggagg cagagagatt tcgtgccatg
     5041 ctcaaagcat ccgacaccgg cactgcctcc agagctgcca gatttccgga taagaagcag
     5101 gtacctacgg caacggccgc cccctctccg ccggagacag ccggtcctgt tttgcccagt
     5161 ctcgacctat caccgtttca agaggcccga aagttccggg tcgagccgac catccggttt
     5221 cggtcggggg acggagacct cgtgaccgac gtcgctgccg ccgtacccga ggatttgacg
     5281 gactacagac tgcgccttgc ataccaccgc ttgcgtctcc ttaaagactt cgaggaactc
     5341 ctgtgtcttg accaactcca gggggtgact cattattggt accaggtgga aacggtcgcc
     5401 agtgtcctca aacggttccg gggacgggtt cttctcgccg acgaggtggg gttgggtaag
     5461 acgatcgaag cgggtatggt catcaaagaa tatctccttc ggggactggc gaggaaggta
     5521 ctcatcctca ccccgcccag tctcgtttcc cagtggcagg aagagatggc agtcaagttc
     5581 gggttgtcct tcgtaacgcc ggatcacgaa gcattccaag gggatagcga acgtttctgg
     5641 agggagcagg acctggtgat cgcttccatc aacaccgcca agtcccagcg gcattctgac
     5701 gtcgtcacca atatcgatta cgacctcgtt gtcgtcgatg aggcacatta cctgcggaac
     5761 aagaagaccc tcaaccatcg cctcgtgggc gccctccgca agcgatttct ccttctcctg
     5821 agcgccaccc ctgtccagaa caacctgata gaactgtaca acctcatcac tctcctccag
     5881 cccggcctcc tcagtaccga agcggccttc aaaagggact acgtcaaacg ggggaatctc
     5941 cggcagccga ataaccccga acaactgcgc aggctcctaa aggaagtgat gatccggaac
     6001 acccgctcta tggtcgacgt caaactgccc aggcgcttcg caacgaccct gtacgtgaca
     6061 ccgacggaag aggagcggga aacgtatgaa cttctgagca ccttaacccg cttgctccac
     6121 gagaaggagg ggccgacgga gctcttgacg atccgaaacc tccagatggc ggccggaagt
     6181 tctcctttcg ccctgaggga tgcccttggt cacctgcagg cccgcctgga aggcgccgac
     6241 catccaatcc gccacctgtt gagggatctc gacgcccggt tggcccgtat agacgactcc
     6301 gccaaaggcc ggcagttcct tgccctggtg gagcagaatc gggagaagaa actgatcttt
     6361 gtccggtacc agaggacgct cgcatatctg gccggtctgc tcagggaaag aggcctaccc
     6421 tttgccgttt ttagcggaac tatgagcaca tacgagaaag acgccgccgt cgaggcattc
     6481 aggcgcgaag tccctattct gctcagcacc gaaaccggcg gtgaaggccg gaacatccag
     6541 ttctgtaaca cgatggtcaa ctatgacatt ccctggaatc ccatgcgcct tgaacagcga
     6601 atcggcagga ttcaccggat cggccaggaa cgggatgtct tcatcttcaa cctctgcctg
     6661 gcaggtagca tcgaggatta catgatgcgg atactggacg acaagatcca catgtttgag
     6721 ctcgtcatcg gcgagatcga caccatcctc ggcaacctgg agaccgaccg cgatttcagc
     6781 agtctcatcc tcgacgtctg gctccgctca ggagacgaag cgctgcggcg ggaaaacttc
     6841 gcccgcctcg aggacgaagt catcgcggcg aagaaggcct atgaaaaaac gaaggccctg
     6901 gacgaaatgc tcttcggcga tgattacgag gtgtaaggtc tgatggaact gcacaaattc
     6961 cttgcgcagt tgctcggccg tcacggctgc ctggtagagg agagtgatga tggtgcatta
     7021 caggtcctat
//
LOCUS       DAOTMX010000046         7005 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_9351_length_7005_cov_3.415827, whole genome shotgun sequence.
ACCESSION   DAOTMX010000046 DAOTMX010000000
VERSION     DAOTMX010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 7005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7005
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9351_length_7005_cov_3.415827"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="P5573_02220"
     CDS             <1..793
                     /locus_tag="P5573_02220"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRT26913.1"
                     /translation="ELFGYEQGAFTDAKKRKIGLLEYADGGTFLLDEIGDMSLPLQAK
                     FLRMLEEGSIRRLGGTDKIPIDVRFIFSTNKDLNRMVAEGSFREDLYYRIRVVPLVIP
                     PLRERQDDIVLLAQHYIDEYNKKFNKKVKGLSREAELILKRYPWPGNVRELKNIIERV
                     MIRQNVGKVLTPDNLPAEIKEDFSSVRIMGGMSRAFAVLPAGRIDYHATVNKVNLEFR
                     KKILEAALERSEGNKAAAARLLGISRYAFLRELKKASKDLPKDLH"
     gene            complement(1328..1717)
                     /locus_tag="P5573_02225"
     CDS             complement(1328..1717)
                     /locus_tag="P5573_02225"
                     /inference="COORDINATES: protein motif:HMM:NF017876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HRT26914.1"
                     /translation="MLFELSVIIFALAFLFFTIFSILYLVQLRQTAKNIESILNKLDQ
                     SLPSIISKIDAIVSDLSETTRMIKMQTSFLANVTRKIEGIVDDIADFEQSLRREIEVP
                     VSNALGTYNALIKGIRAFVFALFSSRP"
     gene            complement(1760..2140)
                     /locus_tag="P5573_02230"
     CDS             complement(1760..2140)
                     /locus_tag="P5573_02230"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HRT26915.1"
                     /translation="MSEGRDFDFLKGLLVGSLIGAAMGILFAPRSGKETREDIRRKSQ
                     EIYSKAKEEYEEAVERSKRAYEGAVERLKEFQAAAKRKAAEAEEKVEELADKGKDALD
                     DTKSRIKRAIDAGVEAYKEEKEKS"
     gene            complement(2224..3099)
                     /locus_tag="P5573_02235"
     CDS             complement(2224..3099)
                     /locus_tag="P5573_02235"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRT26916.1"
                     /translation="MRLLRAIVVISVAALSIISCVAREIPPPPKPTRVALVLGAGASR
                     GFAHIGVLKVLESNRIPVHMIVGTSAGSFVGCVYAFGYSAYQLQRMAIGLDKSDIVDL
                     AIPDNGFIKGEKLENYVNQLVSNTPLERLRIPFYAVATDIRRGEEVVFGMGNTGTAVR
                     ASCSIPGVFRPVSISGATYVDGGVVSPIAVEAARKYGADVVIAVDISAGIGNSVPQTT
                     IETILQSIDIMHSKISSAQLLKADVVITPRVDHIGSADFSKRHEAILEGERAANEALP
                     RIQSILNTFRSQGRL"
     gene            3662..4063
                     /locus_tag="P5573_02240"
     CDS             3662..4063
                     /locus_tag="P5573_02240"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HRT26917.1"
                     /translation="MSDDINRRTQETAARLFGKGIKMEPPYQMWRQFDKELANDLSRH
                     ITGNLYSRTVLTIQERQYAACAMLASLRATAEFKLHVNAALNVGCDPRKITEIIFQVS
                     TYAGMPAVNEALEAYREVLKERGEWPIAPIK"
     gene            complement(4094..4990)
                     /locus_tag="P5573_02245"
     CDS             complement(4094..4990)
                     /locus_tag="P5573_02245"
                     /inference="COORDINATES: protein motif:HMM:NF004283.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HRT26918.1"
                     /translation="MHIQEVVLRDGLQNEKQLVSTEEKLKLIDRLIACGLRQVEITSF
                     VNPHLVPQMADAEDLCNRLQQREGIMFSALILSEKGLYRAMRCGMPQVGVYVSASETH
                     SRRNSNRSIDEALEEARRLIRKARQAGIKVRAGVMNAFGCAYEGKVPIGKVAELSDAL
                     LDAQPNEICLADTSGLGNPLQIKEVLNRVRSVTGVTPLSLHFHNTRGLGLANVFSALE
                     EGIFSFDTSLGGLGGCPFIEGATGNIATEDTVSMLHSMGVETGIDLSALIGVSLDFEK
                     VLGRTFPSLISHLYASLKADGS"
     gene            complement(5094..5309)
                     /locus_tag="P5573_02250"
     CDS             complement(5094..5309)
                     /locus_tag="P5573_02250"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HRT26919.1"
                     /translation="MAVNVVAPMPGTISEILVEVGDQVKADEELIILEAMKMENPITA
                     PVAGTVKEIKVAEEDKVDTNQVLVVLE"
     gene            complement(5442..6632)
                     /locus_tag="P5573_02255"
     CDS             complement(5442..6632)
                     /locus_tag="P5573_02255"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT26920.1"
                     /translation="MATEYDLWKIFPRMPKKVTIGDITVRDGFQHEEKFISTAAKIYY
                     AQEMILAGAREIEITNLGNPAGIPQFRDADQVFAAMRSDSFKKRCERAGVKYDEVVMT
                     AVTIREPAVDRAIELKEKGVGPDRILMMVSTDPEHHFANSGTTLSVYWKEAERCIKKA
                     RDKGIKMCGTVSTIWGSPISGATKLEDAVEFTKRWFSIGAHDIEHADHDGSASAAQVY
                     RYFSMILDAMPNPEAHLCHLHETKRIASASVLAALQAGITRFEGTLGGLGGQPANFLD
                     DCPVRGTGEYYYEDPRYVGLICLEDLLVMIDELGIEHGYDVDRVLLLGRRMEKTCGRR
                     LRSEAVINGRTLKEGHPKFGRPGLKQLIEKLGEKPGQKIPSDWTPEAVLPAKWGPEDP
                     IWKK"
     gene            complement(6711..>7005)
                     /locus_tag="P5573_02260"
     CDS             complement(6711..>7005)
                     /locus_tag="P5573_02260"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRT26921.1"
                     /translation="TARRIDAKTALEIGLVSRVVEPDKLMDAALELAREIAKNGPIAV
                     AQAKFAINYGSEACLGVALPLESKAYEITIPTKDRLEALAAFAEKRKPVFKGE"
BASE COUNT         1676 a         1814 c         1739 g         1776 t
ORIGIN      
        1 cgaacttttc ggttatgagc aaggagcttt caccgacgcc aaaaaacgca aaataggact
       61 gctggaatac gccgacggcg gcactttcct gctcgatgaa atcggggata tgagccttcc
      121 gcttcaggct aagtttctca ggatgctcga agaaggttcc ataaggaggc tcggcggtac
      181 ggataagata ccgatcgacg tcagattcat cttttccacc aacaaggacc tgaacaggat
      241 ggttgccgag ggctctttcc gggaagacct gtactaccgg ataagggtcg tccccctagt
      301 catccctcct ctcagggaga gacaggatga cattgttctt ctcgctcagc attacataga
      361 cgagtacaat aagaaattta ataaaaaggt taaaggcctg agtcgggagg cggagcttat
      421 cctcaaacgc tatccatggc ccggaaatgt gagggagctc aaaaacataa tcgaaagagt
      481 catgatacgc cagaatgtgg gcaaggtcct cactccggat aacctaccgg ctgagattaa
      541 agaagatttt tcttctgttc gtattatggg cggcatgtcg cgggcctttg cagtgttgcc
      601 cgcaggtcgc atcgactacc atgcaactgt caacaaggtc aacttggagt tccgaaagaa
      661 gatcctcgaa gccgccctgg agagaagtga gggcaacaag gcggcagcgg cgcgattgtt
      721 gggaatttct cgttatgcct tcctccgaga actcaagaag gcgtcaaaag atctcccaaa
      781 agatctccac tgaagcaaaa agccgcacta tcagtgcgat aaatgcactg atagtgcggc
      841 atacgcacgt atggtcggca agattcttgt ctgcactgtt tttgtttggt gcgatctacg
      901 gtgcgatatc cgcactatac accaaaaatg gaatgataaa aacctgcaat tcacggcatg
      961 gaagcgtcgg cttcttttac cttcttgcga ttaacgaaat aaatgcattg ttatcaaggc
     1021 gttataattt gccccattag ttggggggaa aaaataagag gtcgcagtag gcacgtctta
     1081 tgcaaaggat agtaaacaga gacctatgat ggcaaacttt agaatgtata aggaaggaag
     1141 gatgatgaac ctgaaagatt gaatgtgccg atctttcagt ttgcatatcg gtggatggtt
     1201 ttagttccct cctttccatc caccgacccc cttcctgaaa ataattctgg agtggctgtg
     1261 agctcgcagg ctcccggtga caatatataa aaaggatggg cggattgaaa aaccgtccat
     1321 ccttttttta tggccgagag gaaaaaagag cgaagacaaa agcacgtatg cctttgatta
     1381 aggcgttata agtgccaagg gcatttgaaa ctggtacctc gatttctcga cgaagagatt
     1441 gttcgaagtc ggcgatatca tccactatgc cctcgatctt ccgcgtaaca tttgcaagaa
     1501 atgaagtctg catcttgatc atccgagttg tctcggaaag gtcgctgacg atagcatcta
     1561 ttttagaaat gatgctcggc agactttggt ctagcttatt taagatgctc tctatgtttt
     1621 tcgccgtctg gcgaagctga acgagataaa gtatcgaaaa gatcgtgaag aaaagaaagg
     1681 ctaaggcgaa aattatgacg ctcaattcaa aaagcatggc ctaagtcccc ttgatgtttt
     1741 gcagtctatc ccgcctctct cagctttttt ccttttcttc tttgtatgct tcgacgccgg
     1801 cgtctatggc cctcttgatt cggcttttcg tatcatcaag ggcatccttc cccttgtcag
     1861 ccaattcctc gaccttttct tcggcttcgg cggctttcct cttcgcagca gcctgaaatt
     1921 ccttcagacg ttctactgct ccttcgtaag ccctcttgct acgttcaacg gcttcttcgt
     1981 attcttcctt cgctttggaa tagatctcct gggattttct tcttatatct tcgcgggttt
     2041 ctttaccact cctgggtgca aacaggatac ccatagcggc gccgatcaag ctgccgacca
     2101 acaatccctt caagaaatcg aaatctctgc cttcggacat ggcttcctcc taaatgtgat
     2161 ttttcttacg caaaaatata atgacagtcg ttttgaaagt caagttaaat gcctggctga
     2221 gtcttaaagt ctgccttgtg agcggaacgt gttcaggatg gattggatgc ggggtagggc
     2281 ttcattggcc gccctttctc cttcaaggat cgcttcgtgg cgtttcgaaa aatcagcgga
     2341 accgatatgg tcgactctgg gagtgattac gacatccgcc ttcaagagct gggcggacga
     2401 tattttggaa tgcatgatgt cgatggattg gagaatagtc tcgatcgtcg tttgaggtac
     2461 ggaatttccg atgcccgctg aaatatcgac agctatgacc acatccgccc cgtactttct
     2521 tgcagcctca acggcaattg gactgactac gcctccatct acgtaagtgg cgccggatat
     2581 gcttaccggc ctgaagacgc cgggaataga acaactcgct ctgacggcag tccctgtgtt
     2641 ccccataccg aacacgactt cctcacccct ccgtatatcg gtggctacgg cataaaaggg
     2701 aatgcgcaac ctctccaatg gtgtattact cactaactga ttgacataat tctcgagttt
     2761 ttcgcctttg ataaatccgt tatccgggat agcaaggtcg acgatgtcgc ttttgtcgag
     2821 gccgatagcc atcctttgaa gctgataggc gctgtatccg aaggcataaa cacaccccac
     2881 aaagctgccc gcacttgtcc caacgatcat atgaaccggt atccggtttg actccaagac
     2941 cttcaggaca ccgatgtgag cgaaaccacg ggatgcccca gcgccgagaa caagcgcgac
     3001 ccttgtcggc ttcgggggag gtgggatttc ccgtgcaaca caggaaatga tcgataatgc
     3061 agcgacggaa atgacaacga tggctctaag aagtctcaca aaatacaacc tttttcggtc
     3121 atttcttgca ggaaggcttt gccgatgatt cgcgtaagag atgatatgtg atgacgaaat
     3181 aaacaaccgg acatcgaatt gtcaaatgcc cggttgttta ttctctcgta acggtcccaa
     3241 tagctacttc ttgggttctt cagccggttt tgccggagcc ggagccgcag gtgcgccagg
     3301 agccggagcc gcaggtgcgc caggagccgg agccggggcc ggagccggag cagtaaccgc
     3361 cggagcttct ttcttcggtt cctcagcctt cttgcaggcg agagtgaagg taaatgacac
     3421 tgcgagaacc agggaaaaca ataataccaa agcccttttc attacttaat cacctccttc
     3481 cgaatagtaa tttccccaaa atcggaggga aagctaaaat ataattacct tgttgtcaag
     3541 agatattttt cgtttccgtt tattgggtga caggtcgtgt cttgacatgg agacggcatt
     3601 taggctaact tcgcctttcc ttaagccatt ttcgaataaa cccacgaagg aggacgttac
     3661 catgagcgat gatatcaaca gaaggactca ggaaacagct gcaagacttt tcggcaaagg
     3721 gataaagatg gagccaccct accagatgtg gaggcagttc gacaaagaac tcgccaatga
     3781 cctttcgcgg catatcacgg gtaatctgta ttcccgtacc gttttgacga tacaggagag
     3841 gcaatacgca gcgtgcgcca tgctggcatc ccttagggcg actgcggagt ttaagctcca
     3901 tgtcaacgcc gcactgaacg tgggttgtga ccccagaaaa atcaccgaaa tcatcttcca
     3961 ggtttcaacc tacgctggaa tgccggccgt gaacgaagcc ctcgaagctt accgtgaggt
     4021 cttgaaagaa agaggagaat ggccgatagc accgataaaa taggtgacga gcagggaaag
     4081 aaagatatag aggtcaagac ccatcggcct ttagactcgc ataaagatga cttatgagtg
     4141 aaggaaaagt ccttcccaag accttttcga agtcgagact tactccgatt aaggcgctca
     4201 gatcgatgcc tgtttcgacc cccatgctgt gaagcatgga caccgtgtct tctgttgcga
     4261 tgttgcccgt cgctccttcg atgaagggac atccaccgag gccgccgagg gaggtgtcga
     4321 acgaaaaaat gccttcttca agggctgaaa aaacatttgc gagaccgagg cctctggtgt
     4381 tgtggaagtg aagtgacagc ggtgtaacgc cggtcaccga cctcactcga ttgaggacct
     4441 cttttatctg cagggggttt cccaatcctg acgtatcggc aaggcagatt tcgttgggtt
     4501 gggcgtcgag gagtgcatct gataattcag cgactttacc gatgggcact tttccctcgt
     4561 acgcacaacc gaaggcgttc ataaccccgg cccttacctt tatgcccgcc tgccgggcct
     4621 tccggatcaa acgtcgagcc tcttcgagtg cctcatcgat cgacctattg ctgtttcttc
     4681 tgctgtgggt ctcgcttgcc gagacgtaga caccgacctg aggcatcccg catcgcatgg
     4741 cccgatagag ccctttctca ctgaggatga gggcagaaaa cattatcccc tcccgttgtt
     4801 gcaaccgatt gcagagatct tctgcgtcgg ccatctgggg aacaagatgc gggtttacga
     4861 aagatgtgat ttcgacctga cgaagaccgc aggcgatcag ccgatctata agcttgagct
     4921 tttcttcagt gctaacgagc tgcttttcgt tctggaggcc gtctcttagg acgacttctt
     4981 gaatgtgcac cttttggggg agatagatca tcctctgcta ccaatgtcaa aaaagatttg
     5041 ccgaaccgcg acggttcggc aaatctctgc atatgacagt gttgctcggc atgctattcc
     5101 aggacaacca aaacctggtt ggtgtctacc ttgtcctcct cggcgacttt gatctccttg
     5161 acagtgccgg caacgggcgc cgtgatcgga ttttccatct tcatggcctc gaggatgatg
     5221 agctcctcat ccgctttcac ctgatcaccc acttctacta aaatctctga tatggttccc
     5281 ggcatgggag ccacgacgtt aacagccatc caacatacct ccttccatat ttatttttat
     5341 tggttagtta cagcctacac tctatgcggc tgtccatcat tcaaggcggg caaaatcgag
     5401 ggagtgggct gcctgcacca ctccctcgcg ataaattccc gctatttttt ccagatcggg
     5461 tcttccggac cccactttgc tggaaggaca gcttccggcg tccaatcgga cggaatcttc
     5521 tgacccggtt tttcaccgag cttttcgatc agttgcttaa gaccgggacg accgaatttt
     5581 ggatgtcctt ctttgagagt acggccgttg atgaccgctt cggaacgaag acgccggccg
     5641 caggtctttt ccatcctcct tccgagcaac aggacgcgat ccacgtcata gccgtgttcg
     5701 atcccgagct catcgatcat gacaagcagg tcttccaggc agatcaaccc gacatacctg
     5761 ggatcctcat agtaatactc acctgtaccc ctgacagggc agtcgtcgag gaagttggcc
     5821 ggctgaccgc ccaagccgcc gagggtcccc tcaaaacggg ttataccggc ctgaagggcc
     5881 gccaagaccg acgctgaggc gatgcgcttg gtctcgtgga gatggcagag atgtgcctcc
     5941 gggttcggca ttgcatccaa aatcattgag aaataacggt acacctgagc tgctgaggcg
     6001 gacccgtcgt ggtcagcgtg ttcgatgtcg tgggcgccga tggaaaacca ccgtttcgtg
     6061 aattccacgg catcctcgag cttcgtcgcg ccgctgatag ggcttcccca gatcgtgctg
     6121 accgttccgc acatcttgat acccttgtca cgcgccttct tgatgcaccg ttccgcctct
     6181 ttccagtaaa cggacagtgt cgttcccgag ttggcaaaat gatgttccgg gtcggtggaa
     6241 accatcatga gaatccggtc cggaccgacg cccttttcct tgagttcgat ggctcggtca
     6301 acagcaggct ccctgatcgt gaccgccgtc atgacgactt cgtcatattt gacccccgcc
     6361 ctttcacagc gtttcttgaa ggaatcgctc ctcatagcag cgaatacctg gtcggcatcc
     6421 ctgaactggg ggatgcctgc ggggtttccc aggttcgtta tttctatttc cctcgctcct
     6481 gcaagtatca tctcctgggc gtagtagatt ttcgccgccg tggagatgaa cttctcttca
     6541 tgctgaaaac cgtctcttac ggtgatatca ccgatcgtta cttttttggg cattcttggg
     6601 aatattttcc aaagatcgta ctccgtagcc atacaaaaac ctccatcata aattgttgaa
     6661 tgttttattc tccctctgaa cgtaacgtta aaagaaagaa cgccaatgtt ttattctcct
     6721 ttgaatacag gcttgcgctt ctctgcaaaa gcggcgaggg cttcaagtct gtcctttgtc
     6781 ggtattgtga tttcgtaagc tttcgattca aggggcaggg caacccccag acacgcctcg
     6841 gagccgtaat tgatcgcaaa cttggcctgt gcaaccgcga tgggcccgtt tttggcaatc
     6901 tcccttgcca gttcgagcgc cgcatccatt agtttatccg gttcgaccac cctgctcacg
     6961 aggccgattt cgagcgccgt ttttgcgtcg attctgcggg ccgtg
//
LOCUS       DAOTMX010000047         6809 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_9685_length_6809_cov_3.835801, whole genome shotgun sequence.
ACCESSION   DAOTMX010000047 DAOTMX010000000
VERSION     DAOTMX010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 6809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6809
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9685_length_6809_cov_3.835801"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /gene="rplV"
                     /locus_tag="P5573_02265"
     CDS             complement(<1..291)
                     /gene="rplV"
                     /locus_tag="P5573_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HRT26922.1"
                     /translation="MEARAVARYVRISPQKVRLVVDLIRGKKVEEADRVLAFTRKRAA
                     GLVRKVLKSARANAANNPNIDEKILYVKEIYVDQGASWRRWRARAMGRAAHIK"
     gene            complement(311..592)
                     /gene="rpsS"
                     /locus_tag="P5573_02270"
     CDS             complement(311..592)
                     /gene="rpsS"
                     /locus_tag="P5573_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HRT26923.1"
                     /translation="MARSIKKGPYVDAKLLEKVRRAEASGDRSIIKTWSRRSTIVPEL
                     IGFTFAVHNGKKFIPVFVTENMVGHKMGEFAPTRTFYSHAGDRKTKVKK"
     gene            complement(596..1417)
                     /gene="rplB"
                     /locus_tag="P5573_02275"
     CDS             complement(596..1417)
                     /gene="rplB"
                     /locus_tag="P5573_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HRT26924.1"
                     /translation="MAIKSYKPTSPGRRFQTGFAFDELTRTGPEKRLVRPLKRTGGRN
                     NRGRVTCWHMGGGHKRRYRVIDFRRDKKDVTAVVKSIEYDPNRSSRIALLCYRDGEKR
                     YILAPLHMAVGDVVVSSDYADIRPGNTMPLRNIPMGSLIHNLELKIGKGAQMIRSAGT
                     YGQIMAKEGAYAQVRLPSGEVRKILLDCKATVGQVGNIEHENVSIGKAGRNRWVGKRP
                     HTRGVAMNPIDHPMGGGEGKSSGGRHPCTPWGVPSKGYKTRKNKTSDKYIIKKRG"
     gene            complement(1430..1714)
                     /gene="rplW"
                     /locus_tag="P5573_02280"
     CDS             complement(1430..1714)
                     /gene="rplW"
                     /locus_tag="P5573_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HRT26925.1"
                     /translation="METYQIIKRPLVTEKTTLAKEANKYHFEVDRRANKIEIGRAVEK
                     LFKVKVVNVRTMNMAGKKKKVGRVLGRKRDWKKAVVTLAPGSSIEIFDKV"
     gene            complement(1714..2337)
                     /gene="rplD"
                     /locus_tag="P5573_02285"
     CDS             complement(1714..2337)
                     /gene="rplD"
                     /locus_tag="P5573_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HRT26926.1"
                     /translation="MPVLAVFDIDKNKVSEVELREDIFGADIRKDLIYEVVRRQLALR
                     RAGTHSTKRRGEVKGGGRKPWRQKGTGRARVGTIRSPLWRGGGIIFGPHPRDYAYPVP
                     KKMRKAALISALSMKASEEKIVILRDFPMAGIKTKAFKEVLDRFGLKKTLFVIDKADP
                     LLEKSSRNIKDVKMMRSEGINVYDLLKYDHLVLLEPSIKMIEGALSA"
     gene            complement(2366..2998)
                     /gene="rplC"
                     /locus_tag="P5573_02290"
     CDS             complement(2366..2998)
                     /gene="rplC"
                     /locus_tag="P5573_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HRT26927.1"
                     /translation="MKTGLIGKKLGMTQVFDDDGLAVPVTVIEAGPCVVIQKKTQSTD
                     GYDAVQLAFGRKKPKNVTKPLQGHQGKVDKGFFRVLREFRISDPDAFEPGQELTAGMF
                     EVGDFVDVTGTTKGRGFAGSVKRHGFRGGKASHGSMLHRAPGSIGASAFPSRVFKGKR
                     LPGHMGHERKTVQNLRVIGVRPESNVILVKGAVPGSKNGIILIRKAVKKS"
     gene            complement(3080..3388)
                     /gene="rpsJ"
                     /locus_tag="P5573_02295"
     CDS             complement(3080..3388)
                     /gene="rpsJ"
                     /locus_tag="P5573_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HRT26928.1"
                     /translation="MADQKIRIRLKAYDYKLLDRSAEDIVDTAKRTGARVAGPIPLPT
                     VINKFCVNRSPHVDKKSREQFEVRTHKRLIDIIEPTQATVDALMKLDLSAGVDVEIKL
                     "
     gene            complement(3412..4605)
                     /gene="tuf"
                     /locus_tag="P5573_02300"
     CDS             complement(3412..4605)
                     /gene="tuf"
                     /locus_tag="P5573_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HRT26929.1"
                     /translation="MAKKKFERTKPHVNIGTIGHVDHGKTTLTAAITKHLSKKGFAQF
                     YSFDMIDNAPEEKERGVTINVSHVEYETDKRHYAHVDCPGHADYIKNMISGAAHMDGA
                     ILVVAASEGPMPQTREHILLARQVQVPAIVVFLNKVDLVDDPELLDLVELELRELLSK
                     YEFPGDKIPIIRGSALKALETDDTKSADAKCVFDLMDAVDAFIPEPVRDLDKPFLMPI
                     GDVFSISGRGTVVTGRVDRGIIKVGEEVEVVGIRPTFKTVCTGVEMFRKTLDEGRAGD
                     DIGVLLRGTKREEVERGQVVAKPGSITPHTKFKAQVYILTKEEGGRHTPFFSGYRPQF
                     YFRTTDVTGVVTLPEGVEMVMPGDNVGMEVHLITPIAMEQGLRFAIREGGKTVGAGVV
                     GTIIE"
     gene            complement(4645..6747)
                     /gene="fusA"
                     /locus_tag="P5573_02305"
     CDS             complement(4645..6747)
                     /gene="fusA"
                     /locus_tag="P5573_02305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRT26930.1"
                     /translation="MAREIPLSHIRNIGIMAHIDAGKTTLTERILYYTGISYKMGEVH
                     DGTAVMDYMEQEQERGITITSAATTCSWKDHRINIIDTPGHVDFTIEVERSLRILDSA
                     VALFSAVEGVEPQSETVWRQADRYRVPRLAFINKMDRVGADFMRVVTVIRERLGANPI
                     PLQIPLGAEEHFRGVVDLVRMEAVTYGRDLQGAEYSVGPVPPEFLEEARQQRDKMIES
                     LSELDEDLMQKYLNEEALSQEEIRRAIRRLTRSMKVIPVLCGSAFKNKGIQLLLDGVL
                     HYLPSPLDMPPVVGKDIRKADRDIVREASDEAPFAALAFKILTDPFVGQLTFFRIYSG
                     VLKAGSYIYNASKDKRERVGRLLKMHANKREDIKEARTGDIVAAVGLNFTTTGDTLCD
                     QAHPLKLETLELPEPVIAIAIEPKTKADEEKMDAALQKIVREDPSFRVRVDRETGQTL
                     ISGMGELHLEIIVDRLSREFGLGANIGNPQVAYKETIRKPVRAEGKYVRQSGGRGQYG
                     HVWIEIEPNEPGRGLVFENKIIGGTVPREFVPAVQKGLHEAMEEGVLAGFPMVDLKVR
                     LVDGSFHEVDSSELAFKIAASVALKNGAKRGNPVLLEPVMSVEVVVPKEYLGDVIGNL
                     TARRGKIVNVESRPGSEAVIAEVPLAEMFGYATDLRSKTQGRATYSMHFLCYNAVSGE
                     LARQIIDTRTTDTGGKRK"
BASE COUNT         1156 a         2169 c         1923 g         1561 t
ORIGIN      
        1 cttgatgtgg gcggcccgcc ccatggcccg cgcccgccac cgtctccagg acgccccctg
       61 gtccacgtag atctccttta cgtacaggat tttctcgtcg atgttgggat tgttcgccgc
      121 gttggcccgc gccgacttca ggactttccg caccagcccc gcggcccgct tgcgcgtgaa
      181 cgccaggacg cgatccgcct cttcgacctt tttcccccgg atgaggtcga ccaccagcct
      241 gaccttctgg ggtgaaatac gaacatatct ggccacagct cttgcttcca tggttcctcg
      301 aaacccgcct ttacttcttc actttggtct tgcggtcccc cgcgtggctg tagaacgtcc
      361 tcgtgggggc gaattcgccc atcttgtgcc ccaccatgtt ctccgtaacg aaaacaggga
      421 tgaatttctt gccgttatgg acggcaaagg taaagccgat cagttccgga acgatcgtgg
      481 agcggcggga ccaggtcttg atgatgctgc gatcgcccga ggcctccgcc cgccggacct
      541 tttccagaag cttcgcatcc acgtaagggc cttttttaat cgaacgcgcc accgcttaac
      601 ccctcttctt gatgatgtat ttgtccgaag tcttgttctt tctcgtcttg tatcccttgc
      661 tgggcacacc ccagggggta cagggatggc ggccgcccga tgattttccc tcaccgccgc
      721 ccatgggatg atcgatcgga ttcatggcaa ccccgcgggt atgggggcgc ttgcccaccc
      781 agcggttccg tccggccttc ccgatgctga cgttttcatg ctcgatgttt cccacctgcc
      841 ctaccgtcgc cttgcagtcg agaaggatct tcctgacctc ccccgacggc aggcggacct
      901 gagcgtaggc gccctctttg gccatgatct gcccgtaggt tcccgccgag cggatcatct
      961 gggccccctt gccgatcttg agctccaggt tgtgaatcag ggatcccatc gggatattgc
     1021 gcagcggcat ggtgtttccc gggcggatat cggcatagtc gctgctgacg accacatcgc
     1081 ccacggccat atggagcggc gcgaggatgt atcgtttttc cccgtcccgg tagcagagaa
     1141 gggcgatccg ggatgagcgg ttcgggtcat attcgatgct cttgacgacg gctgtcacgt
     1201 ccttcttgtc ccgccggaag tcgatgaccc ggtacctccg cttgtgcccg ccccccatgt
     1261 gccagcaggt cacgcgaccg cggttgttcc ggccgccggt tctcttcaac gggcgcacca
     1321 gacgcttctc cggtcccgtc cgggtcagtt catcgaaggc gaacccggtc tgaaaacgcc
     1381 tgcccggtga tgtcggtttg taactcttga tcgccatgtt tccgttcctt tacactttat
     1441 cgaagatctc gatgctgctg cccggcgcca gggtgacaac ggcctttttc cagtcccgct
     1501 tccgacccag aacccgtccc actttcttct tcttcccggc catattcatg gtcctgacgt
     1561 tgaccacctt gaccttgaag agcttctcca ccgcccgccc gatctcgatc ttgttggccc
     1621 gtcggtcgac ttcaaagtgg tacttgttcg cttccttggc cagggtggtt ttttccgtca
     1681 cgagcggtct tttgatgatc tggtaggttt ccattacgcc gacaacgccc cttctatcat
     1741 tttgatggag ggttccagca ggaccaggtg gtcgtatttc agaagatcgt agacgttgat
     1801 cccctccgat ctcatcatct tcacgtcctt gatgtttctc gaggatttct cgagcagcgg
     1861 gtcggccttg tcgatcacaa aaagggtctt cttgaggccg aaacggtcga gaacctcttt
     1921 gaaggccttg gtcttgatgc cggccatcgg gaaatcccgg agaatgacaa tcttctcctc
     1981 gctcgccttc atgctgagtg ccgagataag cgccgctttt ctcatttttt tcgggaccgg
     2041 gtaggcgtag tcccggggat ggggaccgaa gatgatgccg ccgccccgcc acaggggaga
     2101 gcggatcgtg cccacacggg cccggcccgt ccctttctgt cgccagggct ttcgcccgcc
     2161 tcccttgacc tctccccgcc gcttcgtcga gtgggtcccg gccctgcgga gggccagttg
     2221 acgcctcacc acctcgtaaa tgaggtcttt cctgatgtcc gccccgaaga tgtcctcgcg
     2281 gagttccacc tcggatacct tgttcttatc gatatcgaat acggctaaaa ccggcatgtc
     2341 cgacacgctc cagttttttt gtccttcatg atttcttgac ggccttgcgg atcaggatga
     2401 tcccgttctt gctgcccggg acggcgccct tgaccagaat cacgttgctc tccggcctca
     2461 cgccgatcac ccggaggttc tggaccgttt tccgctcgtg acccatgtgg cccggcaggc
     2521 gcttcccctt gaaaacccgg gagggaaagg cgctcgcccc gatggaaccg ggcgcccggt
     2581 ggagcatgga accgtgggaa gccttgccgc cccggaatcc gtgccgcttg acgctgccgg
     2641 caaagccgcg gcccttggtg gtccccgtca catccacgaa gtcgccgacc tcgaacatcc
     2701 cggccgtcaa ttcctgaccc ggttcgaaag cgtcggggtc cgatatcctg aactcacgaa
     2761 gcacgcgaaa gaagcccttg tcgaccttgc cctggtgtcc ctgcaggggt ttggtcacgt
     2821 tcttcggctt cttgcgcccg aaggcgagct gaacggcgtc gtatccgtcg gtcgactgtg
     2881 ttttcttctg tatgaccaca cagggcccgg cctcgatcac cgtgacgggc acggcgagac
     2941 cgtcgtcgtc gaagacctgt gtcattccca gtttcttgcc gatcagtcct gttttcatac
     3001 gcccttctat ctcatgggaa atcataaaaa acaggagacg gattcgaaca tcaaatccat
     3061 ctccatgtct gcggttccgt tacagcttga tttcgacatc cacgccggcg gagaggtcca
     3121 gtttcatgag ggcatcaacg gtggcctgcg tgggttcgat gatgtcgatg agccgcttgt
     3181 gggttcgaac ctcgaactgc tcccgggatt tcttgtccac gtggggagac cggttcacgc
     3241 agaacttgtt gatgaccgtc ggaagcggaa tgggacccgc gacgcgtgct cctgtcctct
     3301 tcgcggtgtc cacgatgtct tccgcggacc gatcgagcaa cttgtagtca tacgccttca
     3361 gtcgaattct tatcttttga tcagccatat cccgcttact cgaagatgaa tctactcgat
     3421 aatcgtaccc accacgccgg cgccgacggt tttcccgcct tcccggatgg cgaaccggag
     3481 tccctgctcc atggcgatgg gggtgatgag atgaacctcc atgcccacgt tgtcaccggg
     3541 catcaccatc tcgacgccct ccggaagggt gaccacgccc gtcacgtcgg ttgtcctgaa
     3601 ataaaactgg ggcctgtaac cgctgaagaa cggcgtgtga cgcccccctt cctccttcgt
     3661 caggatgtag acctgggcct tgaacttcgt gtgaggcgtg atggaaccgg gcttggccac
     3721 cacctggcct ctttccacct cttcccgctt ggttccccgg aggagaacgc cgatgtcgtc
     3781 gcccgcacgg ccctcgtcga gggtcttgcg gaacatctcc acaccggtgc agacggtctt
     3841 gaaggtcggc cggatgccga cgacttccac ttcctcgccg accttgatga tccctcgatc
     3901 cacccggccg gttacgacgg tcccgcggcc ggaaatgctg aacacatcgc cgatgggcat
     3961 gaggaagggc ttgtccaggt cacggaccgg ttccggaatg aaggcgtcca cggcatccat
     4021 cagatcaaag acgcacttcg cgtcggccga tttggtgtcg tccgtttcga gggccttcag
     4081 ggccgaaccc cggatgatcg ggatcttgtc cccggggaat tcgtacttgc tcagaagctc
     4141 ccggagctca agctcgacga gatcgagcag ctcggggtcg tcgacgagat cgaccttgtt
     4201 gaggaaaaca acgatcgcgg ggacctgcac ctgtcttgca aggaggatgt gctctctcgt
     4261 ctgcggcatg gggccttccg atgcggcgac gaccaggatg gcgccgtcca tgtgggctgc
     4321 tccggagatc atgttcttga tgtagtcggc gtggccgggg caatccacgt gggcatagtg
     4381 tcttttatcg gtctcatact ccacgtgaga gacgttgatc gtcacgcccc gttctttttc
     4441 ctccggtgcg ttgtcaatca tgtcgaagga atagaactgc gcgaaacctt tcttggagag
     4501 gtgcttcgta atggcagcgg tcaacgtggt tttcccatga tcgacatgcc cgatcgtccc
     4561 gatgttcacg tgcggcttcg tcctttcaaa ttttttctta gccatctcca tctacctcct
     4621 taccctgata aggtgaatcc gttgttattt cctctttccc ccggtgtcgg ttgttctcgt
     4681 gtcgatgatc tgcctggcca gttctcccga tacggcgttg tagcacagaa agtgcatgga
     4741 gtaggtggcc cgcccctgcg tcttggatct caggtccgtc gcgtaaccga acatctcggc
     4801 cagcggtact tccgcaatga ccgcttccga tcccggccgg gactcgacgt tgacgatctt
     4861 gccccgccgg gccgtcaggt tcccgatcac gtcccccagg tattccttgg ggaccacgac
     4921 ctctacggac atgaccggtt ccaggagcac cgggttgcct ctcttggcac cgttcttgag
     4981 cgccacggag gcggcgatct tgaaagccag ctccgaagag tccacctcgt ggaacgagcc
     5041 gtcgacaagc cggaccttca gatccaccat cgggaagccc gcaagcaccc cctcttccat
     5101 cgcctcgtga aggccctttt gcacggccgg gacgaattct ctcgggacag tgcccccgat
     5161 gatcttgttc tcgaagacca gtccccggcc cggctcgttg ggctctattt cgatccagac
     5221 gtggccgtac tgcccgcggc cgccggactg cctgacgtac tttccctccg cccgcaccgg
     5281 cttgcggatc gtctccttgt aggccacctg ggggtttccg atgttggctc cgaggccgaa
     5341 ctctctggag agccggtcca cgatgatctc caggtgcagc tcccccatgc ccgagatgag
     5401 cgtctgaccc gtttcccgat ccacccggac cctgaaggag gggtcctccc ggacgatctt
     5461 ctggagcgcc gcatccatct tctcctcgtc cgccttcgtc ttgggctcga tggcgatcgc
     5521 gatgacgggc tccggcaact cgagggtttc gagcttcagg gggtgggcct gatcgcagag
     5581 cgtatccccc gtggtcgtga aattcagccc cacggctgcg acgatgtccc cggtccgggc
     5641 ctccttgatg tcctcgcgct tgttggcgtg catcttcaga aggcgtccga ccctttcccg
     5701 cttgtccttg gaagcgttgt agatgtagga acccgctttc aggacaccgg agtaaatccg
     5761 gaaaaatgtc agctgcccga cgaagggatc cgtcaggatc ttgaaggcca gggcggcaaa
     5821 gggtgcttcg tcgctcgcct ccctcacgat gtcgcggtcg gccttccgga tgtcctttcc
     5881 aacgaccggc ggcatgtcca gaggtgaggg aaggtagtgc aatactccgt ccagaagcag
     5941 ctggatgccc ttgtttttga aggccgagcc gcagagcacg ggaatgacct tcatggagcg
     6001 ggtcagacgg cggatcgccc gccggatttc ttcctgggaa agagcttctt cgttcaggta
     6061 tttctgcatc aagtcctcat ccagctcgga gagggactcg atcatcttgt cgcgctgctg
     6121 ccgggcctct tccagaaact ccggagggac aggacccacc gagtactcgg caccctgaag
     6181 gtcccggcca taggtgacgg cctccattcg aacgaggtcc acgacacccc ggaaatgctc
     6241 ctcggcgccg agcgggatct gaagggggat cggattggcc cccaggcgct cccggatcac
     6301 cgtcacgacc cgcatgaagt ccgcaccgac gcggtccatc ttgttgatga aggccaaccg
     6361 cggcacacgg tatcggtccg cctggcgcca gaccgtctcg gactggggct ccacgccctc
     6421 cacggcgctg aagagagcca cggcgctgtc cagaatccgg agcgaccgtt ccacctcgat
     6481 cgtgaagtcc acatgacccg gcgtgtcgat gatattgatc cggtggtctt tccacgagca
     6541 ggtcgtggcc gcggaggtga tggtgatgcc ccgctcctgt tcctgctcca tgtaatccat
     6601 gacggcggtg ccgtcgtgaa cttcgcccat cttgtaggag atccccgtgt agtacaggat
     6661 ccgctcggtc agggtggtct tcccggcatc gatatgggcc ataatgccga tattccggat
     6721 gtgcgatagg ggaatctccc gagccacgtc tcaacgtcca gcagctttta aaagagcgtt
     6781 agaatcgata atgggcaaag gccttgttg
//
LOCUS       DAOTMX010000048         6747 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_9792_length_6747_cov_3.753586, whole genome shotgun sequence.
ACCESSION   DAOTMX010000048 DAOTMX010000000
VERSION     DAOTMX010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 6747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6747
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9792_length_6747_cov_3.753586"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="P5573_02310"
     CDS             <1..340
                     /locus_tag="P5573_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT26931.1"
                     /translation="VLMRFVFHEMSPTLRETVWTELMRVIRPGGLLIVIDFTVPEKPG
                     LLPRLGFELIHFLEHRMDSIYPPHYLHYRDFIDTGGLTAWIAGQGISAAETRRYFGGN
                     LGLVAVSVAA"
     gene            421..903
                     /locus_tag="P5573_02315"
     CDS             421..903
                     /locus_tag="P5573_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRT26932.1"
                     /translation="MQVLIMYYSRTGNTKKLAEAIARGVEEVEGVRSVVKPVSEVTRD
                     DFVESDGIIAGSPVYFGGMVAEMKALFDRFVGIRRKMENKVGAAFATSGDPSGGKETT
                     MMGIIQALLIYGMIIVGDPIDATGHYGVSCVGAPDKQTEANGAKLGRRVATLVKKLRG
                     "
     gene            914..1264
                     /gene="cutA"
                     /locus_tag="P5573_02320"
     CDS             914..1264
                     /gene="cutA"
                     /locus_tag="P5573_02320"
                     /inference="COORDINATES: protein motif:HMM:NF015077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HRT26933.1"
                     /translation="MNDAPIQVLTTTDRKEDAERIARALVDRRLAGCVQILGPVVSTY
                     RWQGKVERAEEWLCFVKTRQDLYGDVEEAIKANHPYEVPEIIVVPVSGGSGDYLKWLD
                     GELKKGPDGKAGQP"
     gene            1261..2517
                     /locus_tag="P5573_02325"
     CDS             1261..2517
                     /locus_tag="P5573_02325"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRT26934.1"
                     /translation="MMVKSIHISNFKSFKDARIENLGLFNVVIGTNAAGKSNFIQVFR
                     FLRDVADHGLRNAVSLQGGRDYVVNSRLATAKPLRLRISYGANLEMKRKGISVLVDEV
                     VYEFAVKFDKKGVGFVILEDSLTSHLRFFRPAGKKKTGRGRPLGEGKGFLFNRQGRID
                     YRLELPAEVPLTSEDLVPQFLKDERLPTDALLLETPFFEVIHNFEKFFSRIAIYDFDP
                     RLPKKAAAMTGKRELEEDAGNLVLVLRDIFSDREKRRKFDNLLKGCLPFVEDIDVEKL
                     GDGSLIFKLKEVYARDSFIPATFISDGTINLTALIVALYFEDKSLLIIEEPEKNVHPY
                     LIGKVVDMMKDAAKRKQIIATTHNAEIVKCAELANILLISRDRNGFSVISRPAERKEV
                     QVFLKNEVGIEDLFAQNLFGQNDDAS"
     gene            2507..3067
                     /locus_tag="P5573_02330"
     CDS             2507..3067
                     /locus_tag="P5573_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT26935.1"
                     /translation="MPRKLFVWVEGASDARFFEAVLRPFLEKRYNSVEVRTYATLKKT
                     KVVNILRGIREAGNDYILVADIDQERCVTAKKEVLVKRFCNLNPGCIRVVVKEIESWY
                     LAGLDDDASSFLGLPVFSATDTVTKEDFNELIPEKFDSRIDFMMEMLKYFLPGVAVGK
                     NRSFAYFIRKHHLDKSILKSDQRRGS"
     gene            3064..4638
                     /locus_tag="P5573_02335"
     CDS             3064..4638
                     /locus_tag="P5573_02335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HRT26936.1"
                     /translation="MKIAGVQEIRDLDREAMEKFCIPEDILMENAGEAAYRVLVKEVG
                     VRGKRFVVLCGTGNNGGDGLVVARKLLSEGGLVKVFIVGDPGKFQGAAKKNFAVASLL
                     PFVAMRQIKTADSIRSDVAHADAAIDALFGTGLSRDVSGLFREVILLLNEKRRPVLSL
                     DIPSGICGDRGAVLGAAVKARWTVTFGVPKRGNLLYPGYEYCGKLYVSAISYPPSVQE
                     RGDIMVSVNDIVPLPPRSREGHKGDFGEVLFVAGAAGYLGAPYFAAHSFLKAGGGYSR
                     LAAPASIAPYIAAKGSEIVLIPLAETKSGSLASRNRDTLLGLAERMDMVVVGPGLSLD
                     REAQDLARDLAANVEKPLIIDGDGLTAVSAKPEILKGRKGPVILTPHPGEMSRLTGKT
                     VGEINEDKIGLLQKTAGELRAVVVLKGAHTLIGYPDGQVFINLSGNPGMAKAGTGDVL
                     TGTIAAMNGLGLPVPEAARMGVFVHGFAGDLAAEARGEDGISAADIMAFLPIALKGVR
                     EGLPDSLREDYEVLRV"
     gene            complement(4661..5635)
                     /locus_tag="P5573_02340"
     CDS             complement(4661..5635)
                     /locus_tag="P5573_02340"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRT26937.1"
                     /translation="MSFRDVLSQYEHEIRMMEDSLKGYLRSEVSLVPEISGHLIGSGG
                     KRFRPLLLLISADLCGYRGDRRYPLSAVIEFIHTATLLHDDVIDHAETRRGKPAANRV
                     WGNTASVLVGDFLYSKSFQLMSGDGDLTVIDLISNTTNTMSEGEILQLQKCGDLDITE
                     KEYLLVIEKKTAVLISAACAVGALIAGAPGAKVDAMAGFGMKLGTAFQLTDDTLDYVA
                     DEEEFGKAVGMDLREGKVTLPLIHTLRCCSPEEKRLIREVVRRSEGDGNAVGEITALI
                     TKYKGVDYALEKARAYVNEAKPFLEAFEDSPPKRALLAVSDYIIDRKL"
     gene            5806..6210
                     /locus_tag="P5573_02345"
     CDS             5806..6210
                     /locus_tag="P5573_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope biogenesis protein OmpA"
                     /protein_id="HRT26938.1"
                     /translation="MKRISLILVVFFLAGCAGQRPVLYPNNHLQQVGEERAQRDISEC
                     MQKAEAYVKANPGAEIIGGAAGGGIMGTIAGSAAGAVDNDLGRGAAIGAASGAAVGLV
                     RGIVKASQPSPVFKAFVNRCLKEKGYEPLGWE"
     gene            complement(6221..>6747)
                     /locus_tag="P5573_02350"
     CDS             complement(6221..>6747)
                     /locus_tag="P5573_02350"
                     /inference="COORDINATES: protein motif:HMM:NF037386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HRT26939.1"
                     /translation="ILRAMKRKGGSAEVRDAVGRIRRMVPEAVLRTSVIVGFPGETAA
                     RFNRLLEFVEEARFENLGAFTYSREEGTEAAGLAYQVSERVKEKRRDLLMERQAAISF
                     EVNRRRIGRVEEVLLEGETGDPVFPLLGRTRTQAPEVDGFTYVRAKKGEIGDIVFCRV
                     VDADTYDLYAEETS"
BASE COUNT         1414 a         2025 c         1976 g         1332 t
ORIGIN      
        1 cgtcctgatg agattcgtct ttcacgaaat gagtcccacc ttacgtgaga cggtctggac
       61 cgaactcatg cgggtcatcc gtcccggcgg gcttttgatc gtcatcgatt ttaccgtgcc
      121 ggaaaaaccg ggacttcttc cccgtctggg gttcgagctg attcatttct tggaacatcg
      181 gatggacagc atctatccgc ctcactacct tcattacaga gatttcatcg atacgggcgg
      241 cctcacggcc tggatcgccg gacaggggat atccgctgcg gagacaaggc gttatttcgg
      301 aggcaaccta gggcttgtcg ctgtttcggt cgcggcatga acaaacattt cccttttaaa
      361 aaaaaatggg atataaattt caagctctta atattgatat tggggggagg aaccgaccgt
      421 atgcaggtat tgatcatgta ttattcccga acgggaaaca cgaagaaact cgccgaggcg
      481 atcgcgaggg gtgtcgagga ggtggaaggc gtccgctctg tcgtaaaacc cgtgtccgag
      541 gtcaccagag atgacttcgt cgagtcggac ggcatcatcg ccggttctcc cgtctatttt
      601 ggcgggatgg ttgcggagat gaaggccctc ttcgacaggt tcgttgggat ccgccggaag
      661 atggaaaaca aggtcggtgc ggcctttgcg acctcgggcg acccctcggg aggcaaggaa
      721 accaccatga tggggatcat ccaggcgctc ttgatttacg gcatgataat cgtcggcgac
      781 cccatcgacg ccaccggcca ctacggcgtt tcctgcgtcg gtgcccccga caaacagacg
      841 gaggcaaacg gcgccaagct cggcaggcgt gtggcgacac tggtcaagaa actcaggggg
      901 tgaaccgggc cccatgaatg acgctcccat ccaggtcttg acgactacgg acaggaaaga
      961 ggatgccgaa aggatagccc gggcgctcgt cgacagaaga ctcgccggct gcgtgcagat
     1021 cctcggcccg gtggtgagta cctaccgctg gcagggaaag gtcgagaggg cagaggaatg
     1081 gctctgcttc gtcaagacgc ggcaggacct ttacggcgac gttgaggagg ccatcaaggc
     1141 caaccaccct tacgaagtcc cggaaatcat cgtcgtccct gtctcggggg gaagcgggga
     1201 ttatctcaag tggctcgacg gagagttgaa aaaaggtcct gacgggaagg ccggtcagcc
     1261 atgatggtaa aaagcatcca catctcgaac ttcaagagct tcaaggatgc ccggatagaa
     1321 aacctgggtc tcttcaacgt cgtcatcggc accaacgcag cgggaaaatc caatttcatc
     1381 caggtcttcc gcttcctgag ggatgtggcc gaccacggcc tgcgtaacgc cgtctccctc
     1441 caggggggac gcgattacgt cgtcaattcg cgcctggcca ctgcaaaacc cctccgcctg
     1501 aggatctctt acggggcaaa cctggagatg aaaagaaagg gcatttccgt cctggtcgat
     1561 gaagtcgtct acgaattcgc cgttaaattc gacaagaaag gcgtcggctt tgtcatcctt
     1621 gaggactcgc tgacgagcca tcttagattt tttcgacccg ccgggaagaa aaaaaccggc
     1681 aggggaaggc ccctcgggga ggggaagggc ttcctcttca accggcaagg ccgaatcgat
     1741 taccgactgg agcttccggc cgaggtcccc ctcacatcgg aagaccttgt ccctcagttc
     1801 ctgaaggatg aacggctacc gaccgacgcc cttctcctcg agacgccttt cttcgaagtc
     1861 atccacaact tcgagaagtt tttcagccgg atagcgatct atgacttcga cccccggttg
     1921 ccgaaaaagg ccgcggccat gacggggaag agggaactcg aggaggacgc gggcaatctc
     1981 gtcttagtgc tccgggacat cttctcggac agggaaaaga ggcgcaagtt cgacaacctc
     2041 ctgaaagggt gcctcccttt cgtggaggac atcgacgtcg aaaagctcgg cgacggctcc
     2101 ctgattttca agctcaaaga ggtttacgcc cgggattcgt tcatcccggc gaccttcatc
     2161 tccgacggga cgataaacct cacggcgctg atcgtggccc tttacttcga agacaagtcc
     2221 ctgctcatca tcgaggagcc ggagaagaac gtccatccct acctcatcgg caaggtcgtc
     2281 gacatgatga aagacgccgc gaaaaggaag cagatcatcg caacgaccca caacgccgag
     2341 atcgtcaagt gcgccgaact ggcaaacatc ctcctcatct cccgcgaccg gaacggcttt
     2401 tccgtcatct ccaggccggc ggagcggaag gaggtacagg tcttcctgaa aaacgaggtc
     2461 gggatcgagg acctcttcgc ccaaaatctt ttcggtcaga acgacgatgc ctcgtaaact
     2521 attcgtctgg gtggaaggcg ccagcgacgc acggttcttc gaggccgttc tcaggccgtt
     2581 cctggaaaag aggtacaaca gcgtggaggt caggacgtac gcaaccctga aaaagacgaa
     2641 ggtggtgaac atattgcggg gaatcaggga ggccgggaac gattacatcc tcgtcgcaga
     2701 catcgaccag gaaaggtgtg tgacggcgaa aaaggaggtg ctggtaaaga ggttctgcaa
     2761 cctcaacccg gggtgcatcc gggtcgtggt caaggagatc gagagctggt atctcgccgg
     2821 gttggacgac gacgcatcgt cattcctcgg cctgccggtt ttttccgcta ccgacaccgt
     2881 caccaaagag gatttcaacg agctcatccc ggagaaattc gattcccgca tcgacttcat
     2941 gatggagatg ctgaaatact ttcttcccgg cgtcgccgtc gggaagaaca ggtctttcgc
     3001 ctatttcatc cgtaagcatc atctggacaa atcgatcctg aaatcagacc aacggagagg
     3061 atcatgaaga ttgccggtgt acaggaaatc agggacctcg accgcgaggc catggagaaa
     3121 ttctgtatcc ccgaagatat acttatggaa aacgctggcg aggcggccta tcgcgtactg
     3181 gtgaaggagg tcggtgtacg ggggaaaagg ttcgtcgtcc tctgcggcac ggggaataac
     3241 ggcggagacg gcctcgtcgt cgccagaaag ctcctatcgg agggcggcct cgtcaaggtg
     3301 ttcatcgtcg gtgaccccgg gaagttccaa ggcgccgcaa agaagaactt cgccgtcgcc
     3361 tccctgctcc ccttcgtcgc gatgaggcag ataaaaacgg ccgattcgat caggagcgac
     3421 gtggcccacg ccgatgccgc catcgatgcc ctgttcggaa cggggctctc ccgggacgtc
     3481 tccgggctct tccgcgaggt cattcttctg ctcaacgaaa agaggcggcc ggtcttgagc
     3541 ctggacatcc cctcgggcat ctgcggtgac aggggagccg tcctgggcgc ggccgtcaag
     3601 gcccgctgga cagttacctt cggcgtgccg aagcggggta acctcctata tccgggctat
     3661 gaatactgcg gtaaactata cgtctccgct atctcctacc ccccgtccgt gcaggagcgc
     3721 ggcgacatca tggtctccgt gaacgacatc gtccccctcc cgccccgaag cagggaaggc
     3781 cataaggggg acttcgggga agtcctcttc gtcgccgggg ccgccggtta tctcggcgca
     3841 ccctattttg cggcccactc ttttctgaag gccggcggcg gctattcacg tctcgccgct
     3901 cccgcctcca tcgcacctta catcgccgcg aaggggagcg aaatagtcct catccccctg
     3961 gcggagacga aatcgggaag ccttgcgagc agaaaccggg acaccctgct gggcctcgcc
     4021 gaaaggatgg acatggtggt cgtcggaccg ggtctctccc tcgaccggga ggcgcaggac
     4081 ctcgccagag acctggcggc caacgtcgag aaacccctca tcatcgacgg tgacggcctg
     4141 acggcggtgt cggcgaaacc ggagatcctc aagggaagga aagggcccgt gatcctgacg
     4201 ccccatccgg gggagatgtc gcggctgacg ggtaagacgg tcggcgagat caacgaggac
     4261 aagatcggcc tccttcagaa aacggctggc gagctcaggg cggtcgtcgt actcaagggc
     4321 gcccataccc tcatcggcta tcccgacggg caagtcttta tcaacctcag cggcaacccg
     4381 ggtatggcca aggcgggaac gggcgacgtg ctcaccggca cgatcgccgc catgaacggc
     4441 cttgggctgc ccgtcccgga agcggcccgt atgggagtct tcgtccacgg attcgcgggt
     4501 gaccttgcgg cagaagcgag gggcgaagac ggcatcagcg ccgccgacat catggccttc
     4561 cttcccattg ctctgaaagg ggtcagggaa gggcttccgg acagcctccg ggaggattat
     4621 gaggttctcc gggtgtgaat gtccataagg aggatgaccg ctacaatttg cggtctatga
     4681 tgtaatccga aaccgccagg agggcacgtt tgggggggga atcttcgaag gcctccagga
     4741 agggcttggc ctcgttgacg taagcccggg ccttctccag ggcataatcg acacctttgt
     4801 acttcgtgat cagggcggtg atctctccga cggcgttccc gtctccttca ctcctccgca
     4861 cgacctccct gatcagcctc ttttcttcgg ggctgcaaca gcgcagtgta tggatcaggg
     4921 gcagggtgac ctttccttcc ctgaggtcca tccccacggc cttgccgaac tcctcttcgt
     4981 cggccacgta gtcgagcgtg tcgtccgtca gctgaaaggc cgtcccgagt ttcatcccga
     5041 accccgccat cgcatctacc tttgctccgg gggcgccggc gatcaaggcg cccacggcgc
     5101 aggcggccga gatcagcacc gccgtctttt tttcgatcac cagcaggtac tccttctccg
     5161 ttatgtcgag gtcgccgcac ttttgcagtt gcaggatctc tccctccgac atcgtgttcg
     5221 tcgtgttcga aatcaggtcg atcaccgtga ggtcgccgtc tccggacatc aactggaagg
     5281 acttcgagta cagaaagtca cccaccagaa cgctcgccgt gtttccccag acgcggtttg
     5341 ccgccggctt cccccgtctc gtctctgcgt ggtcgatgac gtcgtcgtgg aggagcgtcg
     5401 cagtatggat gaattcgatg acggccgaaa gggggtatct cctgtccccc cggtatccgc
     5461 acaggtcggc cgagatgagg agaaggagcg gcctgaaccg cttaccgccg ctgccgatga
     5521 ggtgtcctga tatctccggc acgagggaca cctcggagcg aagatacccc ttcaaggaat
     5581 cctccatcat ccttatctcg tgctcatact gactcaaaac gtcacgaaac gacatgaaat
     5641 cgttactccg tccctaaagc taaccgcttc tatactttaa gcgccgtcgg atgtcaaaat
     5701 tttccttggg cgggcgactt tcgccttgtc cctcggcccc acctgtgcta cgtttctccg
     5761 gtaacaccat ctgtgtcccc ccccgaatta ccgaagaggt gagacatgaa acgtatatcc
     5821 ctcattctgg tcgtcttctt cctggcagga tgtgcgggcc agcgaccggt cctttatccg
     5881 aacaaccatc tgcaacaagt tggcgaagag cgggctcagc gggacatctc ggagtgcatg
     5941 caaaaagcag aggcctacgt caaggccaac ccgggggcgg agatcatagg tggggcagca
     6001 ggcggcggca tcatgggcac gatcgccggc agcgccgcgg gcgccgttga caacgattta
     6061 ggcagaggag cggccatcgg cgcggcgtcc ggggccgcgg tcggcctcgt ccgcggcatc
     6121 gtcaaggcgt ctcagccttc gcccgtgttc aaggcattcg tcaaccgctg cctgaaagag
     6181 aaggggtatg aacccctcgg atgggaatga ctgacggccg tcagcttgtt tcctccgcgt
     6241 acaggtcgta ggtgtcggcg tctacgacgc gacagaacac gatgtctccg atttctcctt
     6301 tttttgccct cacgtaggtg aacccgtcga cctcaggcgc ctgggtgcgc gtcctgccca
     6361 ggaggggaaa cacggggtct ccggtttccc cttccaacag aacttcctcg acgcggccta
     6421 tccgtcttcg gtttacctcg aaggaaatcg ccgcctgtct ctccatcaag agatccctcc
     6481 tcttctcctt gaccctctcc gagacctggt acgccaggcc ggccgcctcc gtcccctctt
     6541 cccgggagta ggtaaaagcc ccgaggtttt cgaaccgtgc ctcttcgacg aactctaaca
     6601 gccggttgaa cctcgccgcc gtttcaccgg gaaacccgac gatgaccgac gtgcgcaaaa
     6661 ccgcctccgg gaccatcctc cggatacggc caaccgcatc ccggacctcg gcgctcccgc
     6721 ccttcctttt catggccctg agaatcc
//
LOCUS       DAOTMX010000049         6739 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_9808_length_6739_cov_3.849042, whole genome shotgun sequence.
ACCESSION   DAOTMX010000049 DAOTMX010000000
VERSION     DAOTMX010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 6739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6739
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9808_length_6739_cov_3.849042"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1161
                     /locus_tag="P5573_02355"
     CDS             <1..1161
                     /locus_tag="P5573_02355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRT26940.1"
                     /translation="AMTELVLYLDKGLNVIWANKAMHEAFHLTPGQLNGKPCYRALHD
                     RGKVCPVCPAEKTLVTGEPHEVFDFSSYGKKWVLRSYPVKNDEGVLTGVVEIVTDITE
                     RREAEEALRRSEQKYRELFENANDMIFILDLEGKIQSYNALVWKTYGYDDKELKGLNI
                     EELLDRAYLPVVRDLIRKKVSDVEVQNPLEFLTYTKDGEAVWVEVNARVIKEKGRPVS
                     IHGIARNITERKRMEEALKKRERELEEQSRNLGDANTALRVLLRRREEDRSELEEKVI
                     CNIRELILPYVENLKMTSIDSRQLNQLKILENNLSEIVSPFLRTLSSKYPNLTPMEIK
                     VINFIKEGRTTKEISGLLNVSPRTVEVHRNNIRRKLRLSNRKANLRSHLLSL"
     gene            1328..1636
                     /locus_tag="P5573_02360"
     CDS             1328..1636
                     /locus_tag="P5573_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT26941.1"
                     /translation="MKYDTDEIIRIKVGRDLSRDYKGFKGTFAALAYNCLHKKALPGF
                     CYEEGLVVHAERDGGTLSLTISGGRPEDSLIEIMDIALSNLSAFPVGPGEAQFSVYVE
                     "
     gene            1691..3136
                     /locus_tag="P5573_02365"
     CDS             1691..3136
                     /locus_tag="P5573_02365"
                     /inference="COORDINATES: protein motif:HMM:NF023222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HRT26942.1"
                     /translation="MALKTAKEYLESIEKMVPRVYIGGKWVKNLLEHPVTRSMVMANS
                     MIYGLAEDPSHKEVMVATSHLTGEPINRNLNVARNIHDLDMRQEMALLTSQTVGTCNY
                     RCVGCDALNSLASTTWEMDQALGTNYNERFNKWLAYAQANDLAVSGAITDAKGDRKKR
                     PSQQDEPDTFVRLVEKRSDGIVVRGIKVSQSGAIGSHETLVLPGGALREGEEAFALAF
                     AVQNSAPGLTYICQYNAYSAEREMYPEEELGNPIYGQRETSTMYFDNVFIPWERVFHC
                     GETKYSGRMVTRFAKAHRMNCGGACKVGFADLIIGGALLAAESIGVEKVPHIQEKIID
                     MLRYSETSHACAIAAAMRGREEPKGSGVFLPDDLFGNAAKLNIAHGFYEIIKNAGDIG
                     GGLVVTMPRQKDLQDPDVGPMLKKAFAAVAPAEKRLKIAKFLQHWSAGLHGPGTWHGA
                     GAPQTQRFMFTVLTDFEAKKKMAKKLMGLKD"
     gene            3380..5068
                     /locus_tag="P5573_02370"
     CDS             3380..5068
                     /locus_tag="P5573_02370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRT26943.1"
                     /translation="MYFAKELLEQSSTLRKKWEDEVRKVIAKNPDQKPERLWTTVSDL
                     PIKRLYTPEDMQDMDFANDIGYPGQFPFLRGNQVTGYRGKYWTFRMFSGMGDARTTNQ
                     RWHLLLKEGQTGLSTAFDFPTLMGYDTDSPKARGECGKCGVAIDTLEDFLVLVDGIPL
                     DKVTTSMTINPPATALWAMYCAAAELKGIPLDKIGGTIQNDMLKEFIAQKTLMCPPEP
                     SVKLISDTVEFGTKYVPRWNTISISGYHIREAGSTAVQELAFTLRDGMEYVEDVMKRK
                     GLDVDDFAPRLSFFFNSHIDFFEEIAKMRAARRIWAKAMRDRYKAKDPRSWWMRFHTQ
                     TAGCSLTAQQPYNNVVRTATEALAAVLGGTQSLHTNSLDEVLCLPSDHAVQIALRTQQ
                     ILAEETGVANTIDPLAGSYFIEALTNEMEQQAWDYIHKIDEMGGMVAAIEKGFPQLEI
                     SDAAYKFQQQIDAGEKIMIGINKYVSEKVEPIPFVEIDDRVEIEQIERLNAVRRKRDN
                     KAVKKCLDDLKNACKKGENVMPYCIEAVKNLASVQEICDVYREVYGEYRDPGLY"
     gene            5102..5506
                     /locus_tag="P5573_02375"
     CDS             5102..5506
                     /locus_tag="P5573_02375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HRT26944.1"
                     /translation="MAKRKLRVMVAKPGLDGHDRGARVLARCFRDAGYEVIYTGCHQT
                     PEQIAAAAIQEDVDMVGLSCLSGAHRYLFPAVKKLLVEKGADDIVVVGGGIIPDQDFQ
                     YLYDNGIKEIFTPGATLESIVKWVNENVKPRN"
     gene            5510..6475
                     /gene="meaB"
                     /locus_tag="P5573_02380"
     CDS             5510..6475
                     /gene="meaB"
                     /locus_tag="P5573_02380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HRT26945.1"
                     /translation="MEKTDKIRAGDVREASRLIRNIEDSVPEAKSAIKHIYPFTGKAQ
                     VIGITGSPGAGKSTLVDGLIECFRKKGKTVGVLAVDPTSPFTGGAILGDRIRMQRHAE
                     DAGVFVRSLATRGALGGLSKAVGDAIHVMDAMGKDVILVETVGSGQQEVEIINHAHTV
                     LVVLIPGMGDEIQAIKAGIMEIADVFVINKADREGSGKLQREIMTMLDMSPIPAGGWR
                     PPIVKIGNAFDPAEFEKGLDELASKILEHREYLINHDLLGDRIRRKRTHELNEALREA
                     ILEPVLNVLVSSGELDRMLDRLQKKEVDPYTLAEEVAKKYLTGCV"
BASE COUNT         1666 a         1729 c         2019 g         1325 t
ORIGIN      
        1 gccatgaccg agctcgtcct ttatctcgac aagggtctca acgtaatctg ggccaacaag
       61 gctatgcacg aggcctttca cttgaccccc ggtcaactta acggaaagcc ctgttacagg
      121 gcgctgcatg accggggaaa ggtctgtccg gtctgccctg ccgaaaagac gctcgtgacc
      181 ggtgagcccc acgaggtctt tgatttttcc tcctacggga agaaatgggt cctccgcagc
      241 tacccggtta aaaatgacga gggcgttctg acgggggttg tcgagatcgt cacggacatc
      301 accgagagga gggaagcaga agaagccctg cgtcgctcgg agcagaaata ccgggagctg
      361 ttcgagaacg ccaacgacat gatcttcatc ctcgacctcg agggaaagat tcagtcctac
      421 aacgccctcg tctggaaaac ttacggctac gacgataaag aattgaaggg gctcaacata
      481 gaggaactgc tggaccgggc ctatcttccg gtggttcggg acctcatcag gaaaaaggtc
      541 agcgatgtcg aggtccagaa tcccctggaa tttctcacct acacgaagga cggggaggcc
      601 gtctgggtgg aggtgaatgc ccgggtcatc aaggagaagg gtaggccggt ctccatccac
      661 ggcatcgccc gcaacatcac agagcgcaag aggatggaag aggccctgaa aaaaagagag
      721 cgggagctgg aagaacagtc ccggaacctg ggagacgcca atacggcgct cagggtgctg
      781 ctgaggcgta gggaggaaga caggtccgag ctggaagaaa aggtaatctg caatatcagg
      841 gagttgatcc tgccgtacgt cgagaacctc aagatgacga gcatcgacag ccgtcagttg
      901 aatcagttga agatcctcga aaacaatctg agcgagatcg tctcgccgtt cctgcggacc
      961 ctctcatcga agtatccgaa cctgacgccc atggagatca aggtaatcaa tttcatcaag
     1021 gaagggcgga cgacgaagga gatctccggc ctcttgaatg tatcgcccag gacggtcgag
     1081 gtgcaccgga acaacatccg cagaaaactg agattaagca acaggaaggc caacctgagg
     1141 tcccatctcc tgtccctgta agacttcatt gtcaggaaac tcgaccgtct ataatactta
     1201 tattatctaa gtatatgatg cgtaattttt ccgtattgat atttcctttg attaatggtg
     1261 ctatctatcg gcccgtgacg gattttaaga accgggcggt taaagagggc agtcctggag
     1321 gtaagagatg aaatacgaca cggacgagat aatcaggata aaagtgggga gggatctgag
     1381 ccgggattac aagggtttca aaggaacctt cgccgcgttg gcttataact gcttacacaa
     1441 aaaggccctt cccggatttt gttatgaaga aggcttggtg gtgcatgccg agagagacgg
     1501 aggaaccttg tctcttacca tctccggcgg tcgcccggag gactctttga tagagatcat
     1561 ggatattgca ctgagcaatt taagcgcctt cccagtgggt ccgggggagg ctcagttttc
     1621 tgtttatgtt gagtaggggg gcggacatac cgaagggtta tcaatcaaac aaagtaaagg
     1681 aggaacggtc atggctttaa aaacggcgaa ggaatacctg gaaagtatcg aaaagatggt
     1741 tccccgcgtt tacattggcg gcaaatgggt aaagaacctc ctggagcatc cggtgacgcg
     1801 gtccatggtc atggcaaatt cgatgatcta cggccttgcc gaagacccga gccacaagga
     1861 ggtcatggta gccacgtccc atctgacggg cgagcccata aaccgcaacc tcaacgtagc
     1921 tcgtaacatc cacgacctgg acatgaggca ggagatggct ctcctcacca gtcagacggt
     1981 gggcacgtgc aattatcgat gcgtcggttg tgatgccctc aacagccttg cctcgacgac
     2041 ctgggaaatg gaccaggcgc tcggcacgaa ttacaatgag aggttcaaca agtggctggc
     2101 ctatgcccag gcaaacgacc tcgcagtatc gggtgcgatc acggacgcca agggcgaccg
     2161 aaaaaagagg ccgtcccagc aggacgagcc cgacaccttc gttcgtctgg tcgaaaagcg
     2221 gtccgacggc atcgtcgtga gggggatcaa ggtgagccag agcggcgcta tcggctccca
     2281 tgagaccctc gtcctcccgg gtggtgccct cagagagggg gaagaggcct tcgccctcgc
     2341 ctttgcggtg cagaacagcg cccccgggct tacttacatc tgccagtaca acgcttattc
     2401 ggcggagcgt gagatgtacc ccgaggaaga gctgggcaac ccgatttacg ggcagcggga
     2461 gacgtcgacg atgtacttcg acaacgtctt catcccctgg gagcgcgtgt tccactgcgg
     2521 tgagactaag tacagcgggc gcatggtgac tcggtttgcc aaggcccacc gcatgaattg
     2581 cggcggtgcc tgtaaggtgg gcttcgccga cctgatcatc gggggcgcgt tgctcgccgc
     2641 tgaaagcata ggtgtggaga aggtacccca tatccaggaa aagatcatcg acatgcttcg
     2701 ttacagcgaa acgtctcatg cctgtgcgat cgcggctgcg atgaggggaa gagaggaacc
     2761 gaagggttcg ggcgtgttcc tgccggacga ccttttcggc aatgccgcca agctgaacat
     2821 agcccacggt ttctatgaaa tcatcaagaa cgcaggggat atcggaggcg gccttgtggt
     2881 aacgatgccg agacagaagg atctgcagga tccggatgtc ggcccgatgc tgaagaaggc
     2941 cttcgcggcg gtggctcctg ctgagaagcg gctcaagata gccaagttcc tgcagcattg
     3001 gtctgccggc ctgcacggtc cgggcacttg gcacggcgcc ggtgcgcctc agacccagag
     3061 gtttatgttc acagtcctca cggatttcga ggccaagaag aagatggcca agaagctcat
     3121 ggggcttaaa gattaagaag gcttcggttc ggtaatgata agagggcaag gcggcagcct
     3181 tgccctttct cgtctccgtc aggacggaga cctaaaaagc atgttttgcc gcggactctt
     3241 atcgtctttt actcttgaca aagattacgt taagttataa caaatatctt agggatggtt
     3301 taagttcgtg ccgttcgagg tgccaaaaaa cactgtcttt atagacagtt tttctaaacc
     3361 aaccaaagga gggagtaaag tgtattttgc aaaagaactg ctggaacagt ccagcaccct
     3421 gcggaagaag tgggaagatg aggtcaggaa ggtcatagcc aagaaccccg accagaagcc
     3481 ggagcggctt tggacgacgg tttctgacct tcccatcaag agactctaca cgcccgaaga
     3541 catgcaggac atggattttg ccaatgatat cggctaccca gggcagtttc ctttcctgcg
     3601 cggcaaccag gtgacgggat accgtgggaa gtactggacc ttcaggatgt tttccgggat
     3661 gggcgacgcc cggacgacaa atcagagatg gcacctcctg ttgaaggaag ggcagaccgg
     3721 tcttagtacg gccttcgact ttccgaccct gatgggctac gacaccgatt ctccgaaggc
     3781 ccggggcgaa tgtgggaagt gcggtgtggc catcgacacg ctggaagact ttctggtcct
     3841 cgtcgatggg attcccctgg acaaggtgac gacctctatg acgatcaacc cgccggcgac
     3901 ggccctgtgg gcgatgtact gcgccgccgc tgaactcaag ggtattcctt tggacaagat
     3961 cggcggcact atccagaacg acatgctcaa ggaatttatc gcccagaaaa ccttgatgtg
     4021 tccgcccgag ccctcggtga aactgatcag cgacacggtg gaattcggca cgaagtatgt
     4081 tccccggtgg aacacgattt ctatcagcgg ctaccatatc cgggaggcag gttccacggc
     4141 agtccaggaa ttggccttca cgctccgtga cgggatggag tacgtggaag acgtcatgaa
     4201 gcgaaaaggg ttggatgtcg atgactttgc tccgcgcctc tctttcttct tcaactccca
     4261 catcgatttc ttcgaagaga tcgccaagat gcgcgctgcg aggagaatct gggcgaaggc
     4321 catgagagac cgttacaagg ccaaagaccc aaggtcctgg tggatgaggt tccacaccca
     4381 gacggcgggt tgttcgctca cggcccagca gccttacaac aacgtcgtcc ggacagcgac
     4441 ggaggccctg gccgccgtac tcggcgggac acaatccctg cacaccaact ccctcgacga
     4501 ggtcctctgc ctgccctctg accatgccgt ccagattgca ctccgaaccc agcagatcct
     4561 agctgaggag acgggtgtcg ccaacacgat cgacccgctg gctggttctt atttcatcga
     4621 ggcgctgacc aacgagatgg agcagcaggc ctgggactac atccacaaga tcgacgagat
     4681 gggcggcatg gtggcagcga tcgagaaggg attcccccag ctcgagatct ccgacgccgc
     4741 atacaagttc cagcagcaga tagacgcggg cgagaagatc atgatcggta tcaacaagta
     4801 cgtctccgaa aaggtcgaac ccattccatt tgtcgagatc gacgacaggg tggagataga
     4861 acagatcgag cgcctcaacg ccgtccgccg taaacgggac aataaggctg tgaagaagtg
     4921 cctcgacgac ctcaagaacg cctgcaagaa gggcgagaat gtcatgcctt actgcatcga
     4981 ggccgtgaag aaccttgcca gcgtccagga gatctgcgac gtctaccgcg aagtctacgg
     5041 cgaataccgt gacccgggac tctattaata attagggatg attcaggagg gagatcagac
     5101 tatggcaaag agaaaacttc gtgtgatggt tgctaaaccg ggtcttgacg gccatgaccg
     5161 cggcgcaagg gtgttggcca ggtgcttccg cgatgcgggc tatgaagtga tttacaccgg
     5221 gtgtcatcag acacccgagc agatagcagc ggcggcgatt caggaagacg tggatatggt
     5281 ggggttgagt tgcctttccg gcgcccatcg ttatctcttc ccggcggtta agaagctcct
     5341 tgtggaaaag ggggcggatg acatcgtggt agtgggcggg ggcatcatcc cggaccagga
     5401 ttttcagtat ctctacgaca acggaatcaa ggagatcttc acgcccggtg cgacgctcga
     5461 aagcattgtt aagtgggtta acgaaaatgt gaaaccaagg aattgataaa tggaaaaaac
     5521 cgataaaatc agggcgggag acgttcgaga ggcatcccgc ctcatccgaa acatcgaaga
     5581 cagtgttcct gaagcgaagt cggctatcaa gcacatttac ccttttacgg gaaaggccca
     5641 agtcatcggt ataactgggt cgcccggagc gggaaaaagc accctggttg acgggttgat
     5701 cgagtgtttc cggaagaagg gaaagacggt cggtgtgctc gccgtcgacc ctactagccc
     5761 tttcaccggt ggtgccatcc tgggtgacag gatccgtatg caacgccatg cggaggatgc
     5821 gggtgtgttc gtgaggagcc ttgccacgcg gggcgccctc ggcggcctgt ctaaggccgt
     5881 gggggatgcg atccacgtca tggatgccat gggtaaggac gtcatcctgg tggaaaccgt
     5941 cggttcggga cagcaggagg tcgagatcat aaaccacgcc cacacggtgc tcgtggtttt
     6001 gataccaggc atgggcgatg aaatccaggc cataaaggcg ggaataatgg aaatcgccga
     6061 cgtcttcgtg atcaataaag cggacaggga agggagcgga aaactgcaaa gggagatcat
     6121 gactatgctc gacatgtcgc ctattcccgc cggaggttgg cggccgccga tcgtgaagat
     6181 cggcaacgcc ttcgacccgg cggaattcga gaaggggctt gacgagctgg cctcaaagat
     6241 ccttgagcat cgagaatatc ttattaacca cgacctcctc ggcgaccgca tccgccgcaa
     6301 aaggacccac gaactgaacg aggcgctccg cgaggcgatc ctcgaaccgg tgctcaatgt
     6361 tcttgtcagc tcgggtgagc tggaccgtat gctcgacagg ctccagaaaa aagaggttga
     6421 cccctatacg ctcgcagaag aagtcgcgaa gaagtatctt acggggtgcg tctgagcgtt
     6481 cagatgttcc aatagtgaca gaaaaaaccc gggatgactg cagcgtatgt tgtagctccc
     6541 gggtttttat aaaaggatgg ggtggccgca gtgactgaag atatcatcca agacacgttg
     6601 tcgaaattca gcagcggctt ggtaatggtc tatacaggcg acgggaaggg taagacgacc
     6661 gccgctatgg gacaggcgct ccgcgccctg ggacacggcc gcaaggcctt tgtcgtgcag
     6721 tttatgaaag ggagaaagt
//
LOCUS       DAOTMX010000050         6719 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_9841_length_6719_cov_3.179772, whole genome shotgun sequence.
ACCESSION   DAOTMX010000050 DAOTMX010000000
VERSION     DAOTMX010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 6719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6719
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9841_length_6719_cov_3.179772"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="P5573_02385"
     CDS             complement(<1..219)
                     /locus_tag="P5573_02385"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRT26946.1"
                     /translation="MKGVVLAGGLGSRLHPLTKVTNKHLLPVYKEPMIYYPIRTLVNA
                     GIDEILIVTGGNNAGDFLRLLGNGSEFGL"
     gene            442..1557
                     /gene="ribD"
                     /locus_tag="P5573_02390"
     CDS             442..1557
                     /gene="ribD"
                     /locus_tag="P5573_02390"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HRT26947.1"
                     /translation="MQKSKDRKPDEYYMKRALRLAARGAGRVSPNPMVGAVVVRDGRV
                     VGEGFHERFGGKHAEINALERASEAARGSTVYITLEPCTHYGKTPPCVDRLIEARPAR
                     VVIGTTDPNPLVSGKGVKTLEKQGIETVVGVLEESCRALNEAYFHFMHTGRPFVTVKF
                     AQSLDGRVATVTGNSRWISSGPSLRLAHRERSLHDAVLVGVGTVLQDDPELTVRLVRG
                     RNPLRVILDSRLRTPLGAKVLKDQDRAKTLVVTTRLGSREKRDELHRVGLETLSLPRG
                     RDGGVSLGRLLDELGKRGVSSLLVEGGSGVITTFLREKRAQRLLAVIAPKIIGKGVET
                     VGDLGIEQVSNALPLYFRKVSRVGDDVVIDARVGEQD"
     gene            complement(1576..3165)
                     /locus_tag="P5573_02395"
     CDS             complement(1576..3165)
                     /locus_tag="P5573_02395"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRT26948.1"
                     /translation="MVRLFDVVLNLGEDESLLPRRAAELLNVPVEHVRVERLVRKSLD
                     ARRNRPPRFVYVLDVSFPDEEGVVERVGGKLKLKPMNESTAPPVVRKAPAPERRPVVV
                     GSGPAGLFASLTLASRGIPVILLERGRPLQQRIADTTSFWEHGILDPESNVNFGEGGA
                     GTFSDGKLTTRLRDSRIGMVKRVLVDFGAPPEILTDGKPHVGTDRLRQVVAEFRRELI
                     RLGCEVRFGAKVSDILVRRGEIAGVVVNGSEEIRTGHLVLAIGQSAADTYRRLMERGM
                     QLEAKPFAMGLRVEHPQELINRIQYGRWCTRPELPPADYFLAVGVPDMNRSVYTFCMC
                     PGGQIIGCSSEKGGVVTNGMSLYSRSSPFANSAVVVNVRKEDFAGPSPLSGISFRRQW
                     EENAFVLGGRDYHAPAQRLTDFVYEKESRDVGPTSFRPGVAVAPLREVLPSFVYGALR
                     KGLVLFEQKMPGFLTSEAVLVGVETRTSSPVRILRGEDGQSLAVRGVYPCGEGAGYAG
                     GIMSSAVDGMKAAERIVNSLA"
     gene            complement(3179..4177)
                     /locus_tag="P5573_02400"
     CDS             complement(3179..4177)
                     /locus_tag="P5573_02400"
                     /inference="COORDINATES: protein motif:HMM:NF002558.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRT26949.1"
                     /translation="MRAAMFNAYLVNDPFGDPGVYVELKFRGEALMFDLGDVHTLPSR
                     KLLKVKSIFVSHTHMDHFIGFDHLLRVCLGRDKRLALFGPPGFMRNVEGKLAAYTWNL
                     VENYTNDFVLSVAEVHPGHVMTGEYRCNEAFRPVVPMAKKDFDGVLLDDPFFLVRAVF
                     LHHKVPCLAFALEEKTRVNIKKNVLEEMGLATGPWLLDLKRRILRNDPDDTPIRAPSA
                     VGGKVFPLGVLRDRVAKLTPGKKIAYVADVLYSEEDARRIEDLARGAEILFIEGGFLE
                     ADAERAAEKHHLTALQSGELAKRAGVKRMVLFHFSPKYQGQGRLLAEEAQRAFEGS"
     gene            4476..4892
                     /locus_tag="P5573_02405"
     CDS             4476..4892
                     /locus_tag="P5573_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HRT26950.1"
                     /translation="MSSPTITPAKERALQERMKRLGISEDDIRETFIRSSGPGGQKVN
                     KTSSGVYLVHLPTGLSVKCQQSRSQAVNRFLARRLLADRIERIQKGVVEAERMRVEKI
                     RRQKKRRSRRAKEKIRQEKHLKSEKKTLRSKVELDE"
     gene            complement(5102..5479)
                     /locus_tag="P5573_02410"
     CDS             complement(5102..5479)
                     /locus_tag="P5573_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HRT26951.1"
                     /translation="MRKVRILAASLLSLFGVLVFATFAMAAQGAAATEGSINYTKAIV
                     VGCSLIAAGLSIGLGANGTGHGMGQATSGSASAVGRNPEAQGKILLTMMVGLAMAESV
                     AIYALVVSLIILCANPLLKVIAG"
     gene            5740..6207
                     /locus_tag="P5573_02415"
     CDS             5740..6207
                     /locus_tag="P5573_02415"
                     /inference="COORDINATES: protein motif:HMM:NF037131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT26952.1"
                     /translation="MMEEEKVKKTGKALQGEIPVEKRPFQMIAEKIGTIEEEVIAAVK
                     KMRDDGRIRKFSAILRHQKAGYTKNAMVLWSVKAEDVEEKGKAFSVSRQVTHCYERTP
                     AFEGRYNIFTMVHAREGEDLLQVVKGLSRATGIDDFKVLESIEEFKKSSMEYF"
     gene            6288..>6719
                     /locus_tag="P5573_02420"
     CDS             6288..>6719
                     /locus_tag="P5573_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRT26953.1"
                     /translation="MGKKNELLFEEAKKYIPGGVNSPVRAFRAVDSTPFFVARAAGSK
                     VYDTEGREYIDYVSSWGPMILGHAYPHVLEAVIKAAERGTSYGAPTEAETVMARMIVD
                     AFPSIDMVRMVSSGTEAAMSAVRLARGFTGKEKVVKFDGCYH"
BASE COUNT         1535 a         1983 c         1858 g         1343 t
ORIGIN      
        1 caacccgaac tccgacccgt tgccgagcag ccgcaggaag tcaccagcgt tgttgccccc
       61 cgtgactatc aggatctcgt cgatgcccgc gttcacgagc gttcggatgg gatagtagat
      121 catcggctct ttgtacaccg ggagcagatg cttgttcgtc accttggtaa gaggatgaag
      181 cctgcttccc aggccgcctg cgagtacaac acctttcatg ttttcccgta ccccttttaa
      241 attctatctc ttacaagccg aaaaatttga aagtttatac catattttct cagctcggcc
      301 aatcgaaaca tgcccttgca gcacatcatc cgagctgtca cgctcccttc atcattgttt
      361 atgaccacgg ggatgtgcta tgaagggcga aaccgccggg ggttagcaga gggcatagag
      421 ctctctgtac accaaggtac gatgcaaaaa tcaaaagacc gaaaaccgga cgaatattac
      481 atgaaacgcg ccctgcgcct cgccgcgaga ggggcgggac gggtcagccc gaaccccatg
      541 gtgggggcag tcgtcgtcag agacgggagg gtcgtcggcg aaggcttcca cgagaggttc
      601 gggggaaaac atgccgagat caacgcactc gaaagggcat ccgaagcggc aaggggttcc
      661 acggtctaca tcaccctgga gccgtgtact cattacggca agacacctcc ctgcgtggac
      721 cgcctcatag aagcgaggcc ggcgcgggta gtcatcggga cgacggaccc caacccgctg
      781 gtatcaggca agggtgtgaa gaccctggaa aaacaaggca tcgagaccgt cgtgggagtc
      841 ctcgaagaaa gttgtcgcgc cctgaacgag gcctactttc actttatgca tacggggcgg
      901 cctttcgtga ccgttaaatt cgcccaatcc ctcgacggga gagtcgccac cgtgacgggt
      961 aattcccgct ggatcagttc cgggccgtcg ctccgcctcg cccacaggga gaggagcctt
     1021 cacgacgctg tcctcgtggg agtggggacc gtcctgcagg acgacccaga gttgacagta
     1081 aggctcgtgc gggggagaaa ccccctgcgt gtgatcctcg attcgcggtt gaggaccccg
     1141 ctcggggcga aggtacttaa agaccaggac agggcgaaga cgcttgtcgt gacgacacgg
     1201 ctgggcagcc gtgagaaacg ggatgaactc caccgggtcg gcctagaaac cctgtctctt
     1261 cccaggggcc gtgacggcgg cgtcagcctc gggaggctcc tcgatgagct gggtaaacga
     1321 ggggtatctt ctcttcttgt agaagggggc tccggggtca tcactacttt cctccgcgag
     1381 aagagggccc agagactcct tgccgtcatc gccccaaaaa taatcgggaa gggcgtcgag
     1441 acggtgggcg acctcggcat agagcaggtg agcaacgccc tccctctgta ctttcggaag
     1501 gtttcccgtg tcggcgacga cgtggtcatc gacgcccgtg tcggcgaaca agactgagag
     1561 cacaagcccg gcatttcatg ccagagagtt gacgattctt tctgcggcct tcatcccgtc
     1621 gaccgcagag ctcatgatgc cgccggcata ccccgcaccc tctccgcagg ggtaaacccc
     1681 ccgaaccgca aggctttggc cgtcctcgcc gcgcaatatc ctcacgggag aggatgtcct
     1741 tgtctccacc cccacgagaa cggcctccga ggtcagaaaa ccgggcattt tttgctcgaa
     1801 cagcaccaaa cctttccgca gcgccccgta gacgaaggac ggcaggacct ccctgagcgg
     1861 ggcgacggcg acgccgggcc taaagcttgt cggccctacg tcacgacttt ctttctcgta
     1921 gacgaagtcc gtcagccgct gcgccggcgc gtggtagtcg cgccccccaa gaacaaaggc
     1981 gttctcttcc cattgtctgc ggaaggagat cccgctcaga ggggaggggc cggcgaagtc
     2041 ctccttacgg acattcacca cgacggcgct gttcgcgaag ggactactcc ggctgtacag
     2101 gctcatgccg ttcgtgacca cccccccttt ttcggaactg caaccgatga tctgaccgcc
     2161 cggacacatg caaaaggtat agaccgagcg gttcatgtcc gggacgccga cggccaggaa
     2221 gtaatccgcc ggaggaagct ccgggcgggt acaccatctt ccgtactgta tccggtttat
     2281 gagttcctgc gggtgctcga ccctcaaccc catggcaaaa ggcttggctt cgagctgcat
     2341 cccccgctcc ataagcctcc tgtaagtgtc cgcggcactc tggcctattg caaggacaag
     2401 atggcccgtc cggatctctt cgcttccgtt gacaacgact cctgctattt ctccccttcg
     2461 gactaagatg tccgaaacct ttgccccgaa acggacttcg caaccaagcc ggatcaactc
     2521 ccgccgaaat tccgcaacca cctgcctcaa ccggtcggtt cccacatggg gtttcccgtc
     2581 ggtcagtatc tcaggcgggg cgccgaagtc gacaagcacc ctcttgacca tgccgattct
     2641 actgtccctg agtctcgtcg tcagtttgcc gtcggaaaaa gtccctgcgc cgccctcacc
     2701 gaaattgacg ttgctctcgg ggtctagtat cccgtgttcc cagaaagagg tcgtgtcggc
     2761 tattcgctgt tgcaggggcc tgccccgctc gaggagaatc acgggtattc cccgggacgc
     2821 caaggtcaga gaagcaaaaa gacctgcggg accgctcccg acgaccaccg gccgcctttc
     2881 aggtgccgga gccttgcgaa cgacaggcgg tgcagtcgat tcgttcatgg gcttgagctt
     2941 aagcttaccc cccacccttt ctaccactcc ttcctcgtcg gggaaggaga catccaagac
     3001 gtagacaaag cggggaggtc tgtttctcct tgcgtcgagc gacttcctga ccaacctttc
     3061 cacccggacg tgttccacag ggacattgag aagctcggcc gcccgccggg gaaggaggga
     3121 ctcgtcctcg ccaagattca aaacgacatc gaataaacgt accatttctt cagaacctct
     3181 atgaaccctc gaacgcccgt tgtgcctcct cggcaaggag ccgcccctgc ccctggtatt
     3241 tgggcgaaaa atggaacaga accatccgtt tcacgccggc cctctttgcc agctccccgg
     3301 attgcagcgc cgtcaggtga tgcttttccg cggccctctc ggcgtcggcc tcgaggaacc
     3361 caccttcgat gaagagaatt tccgccccac gagcaaggtc ctctatcctc cgggcatctt
     3421 cttcgctgta caggacatcg gcaacatagg cgatcttctt ccctggggtc agtttcgcga
     3481 cccgatcccg aagcactccc aaggggaaaa ccttcccgcc caccgccgac ggtgccctga
     3541 tcggtgtgtc atcggggtca tttctcagga ttcgtctctt caggtccaag agccagggcc
     3601 cggtggccaa ccccatctcc tcgagcacgt tcttcttgat gttaaccctc gtcttctcct
     3661 ccagggcaaa ggccagacag gggaccttgt ggtggaggaa gacggccctg accaagaaga
     3721 acgggtcgtc cagcaacacc ccgtcgaaat ccttcttcgc catgggcacg accggcctaa
     3781 aggcctcatt gcaccggtac tccccggtca tcacgtggcc ggggtggact tcggcgacgg
     3841 aaaggacaaa atcattcgtg taattctcca cgagattcca cgtatatgcg gcgagtttgc
     3901 cctctacgtt cctcatgaat cccggtggcc cgaagagggc gaggcgctta tctctgccca
     3961 aacagaccct cagtaggtgg tcgaaaccga taaagtggtc catgtgggtg tgggaaacaa
     4021 aaatgctttt aactttgagg agcttccggg aaggcagggt atggacgtca ccaaggtcga
     4081 acatcagggc ctcccctctg aatttaagct cgacataaac ccccgggtca ccgaaggggt
     4141 cgttaaccag ataggcattg aacatggcgg ctctcatcgg agagcgctat ccaagcggct
     4201 ccgaatctcc tttccctctt acctgacgct ttcttagaga ttttaattcg ttctctcttt
     4261 gcgggcaacc gtatatttcc cgccgcgact taaaaagggt ttgacaatac cgataactca
     4321 agctattcgc aactcagttg ttttgggctt ccggcgtaat cctttgaaaa atcggcctct
     4381 gatacgaaag gttgtataga ggcgaagctc cgcacgacaa gccgcagtgg gttctacgaa
     4441 ggaccccggc gatatccccg ggacgaaatg cgcacatgtc ttctcccacc atcaccccgg
     4501 caaaagaaag ggcgctccag gaacgaatga agcgcctcgg catttccgag gacgacatcc
     4561 gggaaacctt catcaggtcc tcggggcccg gtggccagaa ggtgaataaa acgtcgtcgg
     4621 gcgtttacct cgtccacctg cccacgggct tgtccgtgaa atgtcaacag tcccgttcac
     4681 aggcagtcaa tcgattcctg gcccgccgcc tcctcgcgga caggatagag cgcatacaaa
     4741 aaggcgtggt cgaggcggaa agaatgcggg tcgaaaaaat caggcgccag aagaaaagac
     4801 gctcgcggcg cgccaaagaa aagatacgcc aggaaaaaca cctcaaatcg gaaaagaaaa
     4861 cgctgcgttc gaaggtcgaa ctcgacgaat gatcggcagc cgcttgcccg tctttaaaag
     4921 caacttgata tttgcaaggc gaggaagctg ctccatcgct ctcgcccttg tcggcggaaa
     4981 ggcgctatga gcggatggtc actctcctaa atgcctttcc cggacagaca aaaaagacaa
     5041 aaaaaaggga gaagaagtcc gttttgtttg cttcctctcc cttcgcaaat gaggccgtat
     5101 cttaaccggc gattaccttc aacaacgggt tggcgcaaag aatgatgaga gaaacgacga
     5161 gcgcgtaaat cgcgaccgat tccgccatcg cgagaccgac catcatggtc agcaggatct
     5221 ttccctgtgc ctccgggttt cttcccacgg cgcttgcact gccgcttgtc gcctgcccca
     5281 tgccgtggcc ggttccgtta gcacccaacc caatcgagag tccggcggcg atgagagagc
     5341 atcccacgac gattgccttg gtgtagttga tgcttccctc ggtggccgcc gctccctgtg
     5401 ctgccatcgc aaaagtcgca aacacgagca ctccgaagag actcaaaaga gaagctgcaa
     5461 gtatccttac tttcctcatt tttaccatcc tttcgtttaa gttcgactga aatgccattg
     5521 ccgcttttta ttcctgcttt ttatcccgct ctccttcacc ccccttctac actctttctc
     5581 ctactcaagg ggagctttta gcttcagcaa aaaaaattgt caagaattat ttggttatgt
     5641 tattgtcccc ttcggctttt tctgcctctg aaacaggaaa tgagttgcta gggaagagaa
     5701 aaaaacggca gaaacaaacc tcccggaccg agattgcgga tgatggaaga agaaaaggtc
     5761 aaaaaaactg gaaaagcact ccagggagaa atccccgtag agaaaagacc gttccagatg
     5821 atcgccgaaa aaatcgggac tatcgaggaa gaggtcatcg ccgccgtcaa aaagatgcgt
     5881 gatgacggga ggatccgaaa gttcagcgcg atcctgaggc accagaaggc cggctatacg
     5941 aaaaacgcga tggtcctgtg gtcggtaaag gcggaggatg tcgaagaaaa gggaaaggcc
     6001 ttttctgtgt caagacaagt aacccactgc tacgagagga caccggcctt cgagggaagg
     6061 tacaacatct tcaccatggt ccacgctcgg gagggcgaag acctcctgca agtagtgaaa
     6121 ggcttgtcca gggcgacggg catcgacgac tttaaggtcc tcgaaagcat agaggagttc
     6181 aagaagagca gcatggagta cttttagaat acgatgggtg ataaactgtg aagggtgaag
     6241 accactcctt gtacttcgtg attcacgctc acggtcaggg cgaatagatg ggaaaaaaga
     6301 acgagctctt attcgaggag gcgaagaaat acatccccgg aggcgtgaac agccctgtga
     6361 gggcgttccg ggcggtggac tcgacgcctt tttttgtggc ccgcgctgcg gggtcgaagg
     6421 tttacgacac cgaagggagg gaatacatcg attacgtctc ctcctggggg ccgatgatcc
     6481 tcggtcacgc atacccccat gtcctggaag ccgtgataaa agccgccgaa agaggcacga
     6541 gctacggcgc accgacggag gcggagaccg tgatggcccg gatgatcgtc gacgccttcc
     6601 cgtccatcga catggtgcgg atggtgagct cggggacaga agccgccatg agcgccgtcc
     6661 gcctcgccag ggggtttacc gggaaggaaa aagtcgttaa attcgacggt tgctaccac
//
LOCUS       DAOTMX010000051         6695 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_9881_length_6695_cov_3.542470, whole genome shotgun sequence.
ACCESSION   DAOTMX010000051 DAOTMX010000000
VERSION     DAOTMX010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 6695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6695
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9881_length_6695_cov_3.542470"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="P5573_02425"
     CDS             complement(<1..544)
                     /locus_tag="P5573_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT26954.1"
                     /translation="MKSLVAGGVPPIVSSCVTCAACNQVCPTKANPFDLLNQRQEETG
                     ALGIPAASFENFEKLSSLPTVIKQGEPGKAVMNLCIVGDMVREQLNSPLFKGVTTVEG
                     ADYFCTVGYVHLGKPSPVKREAKRFVEKLSALGVEEIVCFHDDCYALLTTLAEEFGIK
                     VPFRPVHLFEYLYRTLSAMPG"
     gene            complement(807..1868)
                     /gene="dnaJ"
                     /locus_tag="P5573_02430"
     CDS             complement(807..1868)
                     /gene="dnaJ"
                     /locus_tag="P5573_02430"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HRT26955.1"
                     /translation="MAKKDYYEILGVSRGAGEDEVKKGYRRLAMRYHPDKNPGDKSAE
                     EKFKEAAEAYEVLRDPEKRQVYDRFGHEGLNGSGFRGFSGFEDIFSSFGDIFEDVFGF
                     TGARRSKTAARPGADVRYDLKISFLDAVNGVEKDITIERHENCKKCGGSGAAPGTAPE
                     VCPRCRGMGQVTQSSGFFSISTTCPDCRGEGRVIRNACPDCLGVGKVKVNRNVHIKVP
                     AGVDTGSRLRLRGEGQEGKYGGPSGDLYVFIHVEPHEFFQRDGSDVYCRIPISITQAA
                     LGATIEIPTLQGSEKLKIPKGTQSGKMFRLKGKGVPHLRGFGRGDEIVQITVSTPTNL
                     NKRQEELLREFAKAGGESL"
     gene            complement(1987..2880)
                     /locus_tag="P5573_02435"
     CDS             complement(1987..2880)
                     /locus_tag="P5573_02435"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HRT26956.1"
                     /translation="MENGLYSTDFENLKLFSRGKVRDTYEVDDVLLMVATDRISAFDV
                     VMPDPIPGKGKVLTGMSAFWFENTKDIVPNHLITTDVRRFPEPSRLYRETLDGRTMMV
                     KKASALPVECIVRGYISGSAWKDYCESKVVSGIRLPDGLKESSKLESPIFTPSTKARE
                     GDHDTPITRTEMEALVGGTMTEKVIDISIRIYEKASAMAEARGIIIADTKMEFGLTED
                     GNLILIDELLTPDSSRFWPEDEYEEGRPQKSFDKQFLRDYLISIGWDRKPPAPQLPPH
                     IIEKTREKYEEAMTRLVGRPA"
     gene            complement(2906..3535)
                     /locus_tag="P5573_02440"
     CDS             complement(2906..3535)
                     /locus_tag="P5573_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRT26957.1"
                     /translation="MFLKQMQVGHMAVFAYIVGDPETGEGLVIDPAADVDGILREADK
                     KGIRVKYIVNTHGHVDHISGNQEMKKKTGALIVVHEADADMLVSTPAMVLRMFGAKPS
                     PPADITVSDGDTIAVGSVVLEVLHTPGHTPGGICLYTDGYVFTGDTLFVGAVGRTDLP
                     GGSWTVMYRSIKEKILTLPDDTIVLPGHNYGSSPTSTVGREKRTNPFLR"
     gene            complement(3663..5051)
                     /gene="rsmB"
                     /locus_tag="P5573_02445"
     CDS             complement(3663..5051)
                     /gene="rsmB"
                     /locus_tag="P5573_02445"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HRT26958.1"
                     /translation="MKRRRPSRCAKVNPRSLAVEILNRVEEKGAYAEPLLDEVLTKTA
                     MGVQDRRLLTHLVYGTIRARGRYDWIIGRLYERGADALDTGIRNVLRVALHQLLTMDR
                     IPDYAVIDEAVETTKAAQNRAGAGLVNALLRRYMREGKGVSYPDPEKEPTRHLAVLHS
                     HPEWLVEMWVRLLGLEETAELCRANNEIPPLTVRSNRLKVEREVLARRLSEDGCEARY
                     TVYSPDGLILSGLPGSVRRLRAFAEGCFVVQDEASQLVSRLFAPLPGETVIDVCAGSG
                     IKTTHLAALMQNEGRILAMDINARKLALLEELAGRLQAKIIEPVEADALEKPKADLQR
                     AFDRVLVDVPCSGLGTLRRNPEIKWRLTPEEMKKFPPLQKRILANAAQCVKKGGVLVY
                     STCTISDEENEGVVRAFLADNDDFTMVEPSQDIDASLTDGGYFKTYPHRHGTDGFFGA
                     ILRRHEGGGQKN"
     gene            complement(5048..5995)
                     /gene="fmt"
                     /locus_tag="P5573_02450"
     CDS             complement(5048..5995)
                     /gene="fmt"
                     /locus_tag="P5573_02450"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HRT26959.1"
                     /translation="MAAEEKPRILFMGTPEFAVPSLRKLIDERYPVSAVVTQPDRPRG
                     RGRKSEASPVKRTADEYGIPVLQPDKVRDESFLELFREIAPDMVVLVAFGQILPGEVI
                     DFPRLGCLNVHPSLLPLYRGAAPINWALIRGEEKTGVTVIVMDRGVDSGDILLQEEFP
                     IGPEDTYDTLHDRLAEEGARLLVEAVEGVVKGTAKRTPQDHTIATYAPRLKKEDGHID
                     WNAGVKDIINLIRGLSSSPGAYAYLRDKQLKIFFARGEEAQGTGSPGAVVQIGSQELR
                     VAARDGFVYPLDVQIEGRKRMGIQDFLRGYRVMPGEILR"
     gene            complement(6062..>6695)
                     /locus_tag="P5573_02455"
     CDS             complement(6062..>6695)
                     /locus_tag="P5573_02455"
                     /inference="COORDINATES: protein motif:HMM:NF037182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT26960.1"
                     /translation="KTLLALDRGEIDILIGTQMVAKGHDYPNVVLVGVIMADASLNLP
                     DFRAAEYTFQLLTQVSGRGGRGDETGRVIIQTFNPEHYAVRFACGHDYRGFYEEEIVL
                     RKALGYPPFLRLVNVRISGLKKENVEECAREVAEMTRALAKSLAPGESVDIIGPAEAP
                     IPKLKGRYRWQLILKGKSVQSLHGVAQNILSQRRRRGVDVRVDVDPLNFM"
BASE COUNT         1341 a         2048 c         1740 g         1566 t
ORIGIN      
        1 cgccgggcat ggcagaaagg gttctgtaca gatattcaaa aagatggacg gggcggaagg
       61 gaactttgat gccgaactct tccgcgagtg tcgtcaggag ggcgtaacag tcgtcgtgga
      121 aacaaacgat ctcctcgacg ccgagggcgg agagcttctc gacgaaccgt ttcgcctctc
      181 ttttcaccgg gctgggtttt cccaggtgga cataaccgac agtgcaaaaa tagtccgccc
      241 cctccacggt cgtcacccct ttgaaaaggg gactgttcaa ctgctcacgc accatgtcgc
      301 cgactataca aaggttcatc acggccttcc ctggctctcc ttgtttgatg accgtcggaa
      361 ggcttgacaa cttctcgaaa ttttcgaagg acgccgcagg gattcccagg gcgccggtct
      421 cctcctggcg ttgattcaat aggtcaaaag ggttggcttt ggtgggacag acctggttac
      481 aagccgcgca ggtcacgcaa gagctcacga tcggggggac gcccccggcc acaagacttt
      541 tcatctcctc ctgagcgcgt ttcttgtcat attctacata gggacaaaat acaaagcatt
      601 cgccacaaaa atcacatcgt tctggataaa acatcatcaa cctccttaag tattttaaag
      661 agccctgtcc gaaagacgga ctcggaagac gggttccaaa gctcgcgctt cgtaccacat
      721 gcgagctgaa atatcaagcc ggaatgatga ggtttttgga gggatctatc gaaggaaatt
      781 caaacggcgg gtaggggcgg acggggtcat aaagactccc cgcctgcctt cgcaaattct
      841 ctcaggagtt cctcctgacg cttgttaagg ttagtcgggg ttgacacggt tatctggacg
      901 atttcgtcgc cccttccgaa gccccttagg tgaggaacgc ccttgccctt cagtcggaac
      961 atcttgccgc tctgcgtgcc cttgggaatc ttcagcttct cagacccctg gagggtgggg
     1021 atctctatgg tcgccccgag ggccgcttgg gtgatcgaga ttggtatccg gcaataaaca
     1081 tcacttccgt ccctctggaa gaactcatgg ggttcgacat ggataaagac atagaggtcg
     1141 ccgctcggac caccgtattt tccctcctgc ccttcccctc gcaaccgcag ccgtgaccct
     1201 gtgtcgacac cggcaggaac cttgatgtgc acgttcctgt tgactttcac tttcccgact
     1261 cccagacagt cggggcaggc gttcctgatg acccttcctt caccccgaca atccgggcag
     1321 gtcgtgctga tgctgaagaa gccgctcgac tgcgtcactt gtcccatacc gcggcaccgc
     1381 gggcagactt cgggggccgt accaggagcc gcgccgctcc caccgcattt cttgcagttt
     1441 tcgtgtcttt cgatggtgat gtctttttcg acgccgttta cagcatcaag aaaggaaatc
     1501 ttcaggtcat agcgtacgtc ggcacccggg cgggccgccg tcttcgacct ccttgccccg
     1561 gtaaagccga aaacgtcttc aaagatatcg ccgaaactcg aaaaaatgtc ttcgaaaccc
     1621 gagaaaccgc ggaaaccgga cccgttcagg ccttcatgtc cgaatcggtc atatacctgt
     1681 ctcttctccg ggtccctgag gacttcgtac gcctcggcgg cctccttgaa cttttcttct
     1741 gccgatttat ctccggggtt cttgtcgggg tgataacgca tggcgaggcg gcggtaaccc
     1801 ttctttacct cgtcctctcc ggcgcccctt gaaaccccca agatttcgta ataatctttc
     1861 tttgccattc ttaatacaag actctcaagc accgtggccg atcagtttcg ccgccgttcg
     1921 ggtgcattaa cggtgaatat tcgtcgacat cactatatat taataatccc cgactcgatg
     1981 tcaaggtcag gctggacgcc cgacgaggcg ggtcatagct tcttcgtatt tttccctcgt
     2041 tttttcgatt atatgggggg gaagttgcgg ggctgggggt tttcggtccc atccgatcga
     2101 gatgagatag tctctgagaa attgcttgtc aaaactcttc tgcggccttc cttcttcgta
     2161 ttcatcttcc ggccagaacc ttgaagaatc aggggtaaga agttcatcta tcaaaatcag
     2221 gttgccgtcc tcggtgagac cgaattccat cttcgtgtcg gctatgatta tgccccttgc
     2281 ctccgccata gctgaggcct tttcgtaaat cctgatgctt atgtcgatca ccttctccgt
     2341 catggttccg ccgacgagcg cctccatctc cgtcctggtg atcggtgtgt cgtggtcgcc
     2401 ttcccttgcc ttcgtcgaag gagtgaagat cggactctcc agtttcgacg actctttgag
     2461 gccgtcggga agcctgatgc ctgaaacgac cttcgattcg cagtagtctt tccaggcgga
     2521 gccgctgatg taacccctga cgatgcattc gacgggaagc gcagaggctt tcttcaccat
     2581 catcgtccgg ccgtcaagcg tctcccggta aaggcggctg ggctcgggga accggcggac
     2641 gtccgttgtt atcaggtggt tcggtactat gtccttcgtg ttctcgaacc agaaagcaga
     2701 cataccagtc aggaccttgc ctttgcccgg gatcggatcg ggcatcacca catcaaaagc
     2761 tgaaatcctg tccgtcgcca ccatcagcag gacgtcgtcc acctcgtagg tatcccggac
     2821 ctttcccctg ctgaaaagct tgaggttctc aaagtccgta ctgtacagcc cattttccac
     2881 agcttatcct ttttctgacg gttttttatc tcaaaaaggg attggtcctc ttctcacggc
     2941 cgacagtcga tgtaggcgac gacccgtagt tatgtccggg aagcacgatc gtgtcgtcgg
     3001 ggagggtcag gatcttttcc tttatcgagc ggtacatgac tgtccatgat cctcccggaa
     3061 ggtccgtcct gccgacggct ccgacaaaaa gggtgtcgcc cgtgaatacg tacccatccg
     3121 tatagaggca tatccccccg ggtgtatggc cgggcgtgtg taggacctcg agcaccacgc
     3181 tccccactgc gatcgtatca ccgtcactca ccgtgatatc cgccggcggc gacggtttgg
     3241 ccccgaacat cctgagtacc atcgcgggag tcgacaccag catgtccgcg tcggcttcat
     3301 ggacaacgat caaggcgccc gtctttttct tcatctcctg gttgcccgat atgtggtcca
     3361 cgtgaccgtg ggtgtttacg atgtacttga cccgaatccc ctttttatcg gcttccctta
     3421 gtatgccgtc gacgtcggcc gccgggtcta tgaccagccc ctcaccggtc tccgggtcac
     3481 ccactatgta agcgaatacg gccatgtggc cgacctgcat ctgctttaga aacatataaa
     3541 cccctgttta gagtccagat tcaaagttca aattccgacg tttggaaacg cctgactgcc
     3601 gaagaccaag cgttgacagc ctctcgacga ggggcgtttt tagcattaag agcaccggta
     3661 agtcaatttt tttgcccgcc accttcatgc ctcctgagaa tggcgccgaa gaatccgtct
     3721 gtgccatgtc tgtgcggata ggtcttgaaa taaccgccgt cagtgagact tgcgtcgatg
     3781 tcctgcgagg gctccaccat agtgaaatca tcgttatccg caagaaaagc ccgaacgact
     3841 ccttcgtttt cttcgtcgga aatcgtgcag gtgctgtaaa caaggacccc tcccttcttc
     3901 acgcactgcg ccgcgtttgc caggatgcgt ttctgcaggg gaggaaattt tttcatctct
     3961 tccggcgtaa gccgccactt gatctccggg ttccgccgca gagtgcctag gcccgagcag
     4021 ggcacgtcga caaggacccg gtcaaaggca cgctgtagat cagccttagg cttttccaag
     4081 gcatccgcct cgacgggctc gatgatcttt gcctgaagcc tgccagccag ctcctcgagc
     4141 aaggctagct tacgggcatt gatgtccatg gctaaaatcc ttccctcgtt ctgcatcagc
     4201 gcggcaagat gggtcgtttt tatacccgac cccgcacaga catcgatgac cgtctccccc
     4261 ggcaacggcg caaacagacg agaaaccaac tgagacgcct catcttgaac cacgaagcaa
     4321 ccttcagcga aggcacgcaa ccttctgaca gatccgggaa ggccggagag tatgagtccg
     4381 tcgggggaat aaacggtgta acgggcctcg cagccatctt cggaaagcct acgggcgagg
     4441 acttccctct cgactttgag ccgattgctc ctcactgtga gcggcggtat ctcattgttt
     4501 gccctgcaca attcggcagt ctcttcaagg cccagaagtc ggacccacat ctccaccaac
     4561 cattccggat gagaatggag caccgcaaga tgccgcgtcg gttctttctc ggggtcggga
     4621 tacgagacgc ccttcccttc cctcatatac cttcggagaa gggcgttcac aaggccggcg
     4681 cctgcccgat tctgcgccgc tttcgtcgtt tcaacggcct cgtcgatcac ggcataatct
     4741 ggtatccggt ccatcgtaag gagctggtga agggccaccc tgaggacgtt tctgataccg
     4801 gtgtccaggg cgtccgcgcc tctttcgtaa agccgtccga ttatccagtc ataccggcct
     4861 cttgcacgga tggtcccgta aacaagatgg gtgaggagcc tgcggtcctg gacacccatg
     4921 gccgttttcg tcaacacctc gtcgagcagg ggctccgcat aggcgccctt ttcttcaacg
     4981 cggttgagta tctcaacggc gagactcctc ggattcactt ttgcacagcg actgggcctt
     5041 cttctcttca ccgaagaatc tcccccggca tcaccctgta tcccctgagg aaatcctgga
     5101 ttcccattct cttcctgccc tctatttgca cgtccagagg atagacgaag ccgtcccgcg
     5161 ccgccacgcg tagttcctgc gacccaattt ggacaacggc ccccggcgac ccggtccctt
     5221 gcgcttcttc tccgcgggca aaaaatatct tgagctgttt gtctctcagg tatgcatagg
     5281 cacccggcga tgacgacagg ccgcggatta gattgatgat gtccttcacg ccagcgttcc
     5341 agtcgatgtg gccatcttcc tttttcagac gcggcgcata ggtcgcaatc gtatgatcct
     5401 gtggtgtcct cttcgccgtc cctttcacca caccctcgac cgcctcgacg agcaggcgtg
     5461 ccccttcttc cgccagccgg tcatggagtg tgtcgtatgt gtcttccggc ccgatcggaa
     5521 actcttcctg gagcaggatg tcgcccgaat ctacgcccct atccatgacg attactgtca
     5581 caccggtctt ctcctcgccc cggatcaggg cccagttgat gggtgcagct cccctgtaga
     5641 ggggcaaaag tgacggatgt acgttgagac agcccagccg cggaaaatcg atgacctccc
     5701 caggcaggat ctggccgaag gcaaccagaa ctaccatgtc gggggcgatc tctcggaata
     5761 actcgagaaa cgattcgtcc cgcaccttgt ccggttggag caccgggata ccgtattcgt
     5821 cggccgtcct cttcacgggg gacgcctcgc tcttgcggcc cctcccgcgg ggacggtccg
     5881 gctgggtgac gacggccgac acgggatagc gttcatcgat caactttcta agcgacggga
     5941 ctgcgaattc cggcgtaccc ataaaaagaa tcctcggctt ctcctcagcg gccataagcg
     6001 acctcctctc gacctccccg gcctcggtca catccgcagt ccggggcttt cccttgaatc
     6061 atcacatgaa attcaaaggg tcaacatcga cccgaacgtc gacccctctc cggcgacgtt
     6121 ggctcaaaat gttttgcgcc accccatgga gcgactgtac actcttgccc ttcaggatca
     6181 gttgccaccg gtacctaccc ttgagcttag ggatcggcgc ctccgccggc cctattatgt
     6241 ccacactctc tcccggcgcc agcgacttcg ccaacgccct agtcatttcg gcaacctctc
     6301 gggcgcattc ttctacgttt tcctttttca gacccgatat tcggacattg accagccgca
     6361 aaaaaggcgg atagcccagg gccttccgga ggacgatctc ctcttcgtaa aaaccgcggt
     6421 agtcgtgccc acaggcgaaa cgaacggcgt agtgctccgg gttgaaggtc tgaatgatca
     6481 cccgcccggt ctcgtctccc ctcccccccc ggccggagac ctgagtcaag agctggaagg
     6541 tgtactcggc cgccctgaag tcggggaggt tgagcgaggc atcggccatg atgaccccga
     6601 ccagcacgac attgggatag tcgtgtcctt tcgctaccat ctgtgtccca ataaggatat
     6661 cgatctcgcc cctgtcgagg gcaagcaggg tcttt
//
LOCUS       DAOTMX010000052         6683 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_9909_length_6683_cov_4.214243, whole genome shotgun sequence.
ACCESSION   DAOTMX010000052 DAOTMX010000000
VERSION     DAOTMX010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 6683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6683
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9909_length_6683_cov_4.214243"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2257
                     /gene="fdnG"
                     /locus_tag="P5573_02460"
     CDS             <1..2257
                     /gene="fdnG"
                     /locus_tag="P5573_02460"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HRT26961.1"
                     /translation="DYHVPLRSGTDIAFLGGMIHYILTNHKYFRDYVVNYTNASFIVS
                     KDFSFNDGLFSGYDPAKRKYNKDTWVFEKDDKGVPKRDMTLNDPQCVFQLLKKHFSRY
                     TLDKVSSITGVSKENLTKVYAEYSATGVPDKAGTECYALGWTHHTTGSQIIRTMSIIQ
                     LLLGNMGIAGGGVNALRGEPNVQGSTDHAILYNILPGYLKMPPASLDTLEKYLEKNTP
                     KSNDPQSANYYQNYPKFTVSFLKSMFGDKAVKENDFGYSWLPKVDDGVAYSLMHLFDK
                     MYEGKVKGLFVVGTDPAVSSPNTAKVRKALEKLDWMVGENIFDNETYSFWKGPGVDPK
                     KVKTEVFLLPASATMEKEGSQSNSGRWVQWKYKAAESPEDAIPVGEITIKIMAAVKAL
                     YAKEGGACPAPILNLKWDYTDAKGRYDALKVSKQINGYFLKDTVVDDKGVKTLYKKGQ
                     TVPTFGMLQADGSTSSGNWIMAGSFDAKGLNKMTLRGKDDPTGLGLYPNWSYAWPLNR
                     RIIYNRASCDINGKPYNPKRRLLEWTGDKWVGDVPDGPWPPMADKAKGKYPFIMKPDG
                     LGALFGPGMVEGPFPEHYEPLEGPLTKNPLSGQLINPAIEIFKSDMDKVANADAKFPY
                     VCTTYSCTEHWCSGALTRWQAWLLEMQPELYVEIGEKLAQEKGIQNGSRVKVQSVRGQ
                     VECVAMVTKRFQPFTVEGKTVHQVGLPFNYGWLFPKDGGDSTNFLTPTVGDANTFCPE
                     YKAFMVNVVKL"
     gene            2269..3075
                     /locus_tag="P5573_02465"
     CDS             2269..3075
                     /locus_tag="P5573_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRT26962.1"
                     /translation="MSTSEVIKLIDTTRCTACRGCQVACKQWNELPGLRTKNRGSYQN
                     PPDLGWNTWTLIRFQEYVNEKGTFKWLFRKDGCMHCTDAGCVKVCPSGALYHTEYGTV
                     GIKKDLCIGCKECTAACPFEIPRYNRRTDKMTKCDLCLTRLQANQQPACVKSCPTGAL
                     SIGPKDKMLTAAYDRVKVLGGDANVYGDKFLGGTHVLYVLSEKPEVYDKLPKSPSIPL
                     SVTVWKDWLKPLTWIAGGGVIAGSLLHYILHGPKRPYPEVDQGDHAERGK"
     gene            3079..3747
                     /locus_tag="P5573_02470"
     CDS             3079..3747
                     /locus_tag="P5573_02470"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HRT26963.1"
                     /translation="MPEKGMIKATDLFERVVHWMLAGSCILLFITGLGMMFRELNFIG
                     NFMGGLVGLKYVHNFTGIFFIVSLVLAARMWWREAGKFEFPDDIEWIKQAGGYLWRVD
                     HVAEAGKYNAGQKMFFLTVVGCGALMGVSGLIMWFPMGLPMGLVRWMYPLHAFGFVVL
                     FAFFFVHLYLGTIGSPGSLPAMLDGWVTRAWAKTQHPKWLKEMEAAGKLEVYGEEKKE
                     AVSH"
     gene            3932..4687
                     /gene="modA"
                     /locus_tag="P5573_02475"
     CDS             3932..4687
                     /gene="modA"
                     /locus_tag="P5573_02475"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HRT26964.1"
                     /translation="MKRILTKVFGSILVALFLSCPLPSQASENLKAAVAANFMAPFKE
                     ITAAFEAETKVKVEATFSSTGSLYAQIINGAPYDVFLAADEVRPRILFGKKLSGEPFV
                     YATGRLVLWGAGKNFCGRADWKDALSAEDIRKIALANPETAPYGAAARQALRQVGLWK
                     KLEGRVVTAQNLGQAFQYALMGGVDASFCALSSALTPEGEKGCYYVVEEAPPIRQAAC
                     LLKNSARQKEAGEFLRFLSSPKAEAIKKRYGYH"
     gene            4687..5337
                     /gene="modB"
                     /locus_tag="P5573_02480"
     CDS             4687..5337
                     /gene="modB"
                     /locus_tag="P5573_02480"
                     /inference="COORDINATES: protein motif:HMM:TIGR02141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HRT26965.1"
                     /translation="MDFLPLYLSAKLAVITTSVLILISAPLAYALVYAKWPGKFLFEA
                     LVNLPIALPPTVTGLYLLYLLGPQGYVGAIWKEVFGGTLLFTFAGIVIATAVYNLPFA
                     VQPIKATFEKIDPRLRENAYVLGISPVATFFRVMIPNCINGIAAAAILVFLHSLGAFG
                     VVLMVGGSISGETRVASIAIYEAVETMNFSEAWILSLAFLPVSYFLLLLVNRLTRR"
     gene            5342..6055
                     /locus_tag="P5573_02485"
     CDS             5342..6055
                     /locus_tag="P5573_02485"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRT26966.1"
                     /translation="MGLRAILRKKTEYFSIDVDFFCPEGKTLVLIGPSGAGKTTIIRM
                     LAGLDRPDDGRITFRGKVIFDADRDICLPPQKRDLGYVFQDFTLFPHLTIHGNASFAA
                     KDKNDVDHLINYFGLAHLRDRKPHQVSGGERQRCAICQALARRPDLLLLDEPFSALDV
                     VTRRGLRAELKKIKEERGLSVVFVTHDIHEALFIANSILPIVDGKVDRDWLEHITSPS
                     SPRDAQPRAVREPKLALVY"
     gene            6071..6646
                     /locus_tag="P5573_02490"
     CDS             6071..6646
                     /locus_tag="P5573_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit E
                     family protein"
                     /protein_id="HRT26967.1"
                     /translation="MKIGTYSYEEYIHLIKSFHGNPAPGLLIGGFMVDEALRRLPSGE
                     FFDAICETYTCLPDAVQILTPCTVGNGWLKVFDYGRFALALYEKYSGEGIRVYLDPAK
                     LDPWPEIKGWFLKLTPKANQDSQKLFDEIKEAGTNILSFHKVRIRTENLKKKKMGPVG
                     VCPSCGEAYPLRDGSVCRPCRGESPYVKEES"
BASE COUNT         1511 a         1864 c         1912 g         1396 t
ORIGIN      
        1 cgattaccat gtccccctcc gctccgggac ggacatcgcc ttcctgggcg gcatgatcca
       61 ttacatactg accaaccata agtatttcag agactacgtc gtaaactaca ccaacgcgtc
      121 cttcatcgtc agcaaggact tctctttcaa tgacggcctc ttctccgggt acgacccggc
      181 gaaacggaag tacaacaagg acacctgggt cttcgaaaag gacgacaagg gcgtaccgaa
      241 gcgagatatg acgcttaacg atccccagtg cgtcttccag ctgctgaaga aacacttctc
      301 ccgttatacc cttgataagg tttccagcat cacgggtgtc tccaaggaaa atctgacgaa
      361 agtctatgcc gagtacagcg ccacgggtgt cccggacaag gccggcaccg aatgttacgc
      421 cctcggttgg acgcaccata cgacgggaag ccagatcatc cgtacgatgt caatcataca
      481 gctcctcctg ggcaacatgg gtattgccgg tggcggggtg aacgccctac gcggcgagcc
      541 gaacgtccag gggtcgacgg accatgctat cctttacaat atcctgcccg gatacttgaa
      601 gatgcctccg gcgtccctgg atacgctgga gaaatacctg gaaaaaaaca ccccgaagtc
      661 gaacgacccc caatcggcga actattatca gaattacccg aagttcaccg tcagcttcct
      721 gaaatcgatg ttcggggaca aggccgttaa agaaaacgat ttcggttatt cctggttgcc
      781 caaggtcgac gacggcgtcg cctattccct gatgcacctc ttcgacaaga tgtacgaggg
      841 caaggtaaag ggtcttttcg tcgtcgggac ggacccggcc gtcagttctc cgaatacggc
      901 caaggtgcgc aaggccctcg agaagctcga ctggatggtc ggcgaaaaca tcttcgacaa
      961 cgagacctat tccttctgga aagggcccgg tgtcgacccc aagaaagtca agacagaggt
     1021 cttcctcctt ccggcctccg ccaccatgga aaaagaggga agtcagagca acagcggccg
     1081 ctgggttcag tggaagtaca aggccgcgga atcgccggaa gacgccatac ccgtagggga
     1141 gatcacgata aagatcatgg cggccgtcaa ggccctctat gccaaagagg gcggtgcttg
     1201 tccggctccc atcctgaacc tcaagtggga ttacaccgat gccaagggta ggtatgacgc
     1261 cctgaaggtg tccaagcaga taaacggcta ctttctcaag gatacggtcg tcgatgacaa
     1321 gggagtcaag acgctgtaca agaagggaca aacggtcccg accttcggca tgctgcaggc
     1381 cgacggaagc acctccagcg gcaactggat catggccggc agttttgacg ccaaggggtt
     1441 gaacaaaatg acccttcggg gaaaggacga ccccacggga ctcggccttt atcccaactg
     1501 gtcctacgcc tggcccctca acaggaggat catttacaac cgtgcctcct gcgacatcaa
     1561 cgggaaaccg tacaatccaa aacgcaggct cctggaatgg acgggcgaca agtgggtagg
     1621 agacgtacct gacggcccat ggccacctat ggcggacaag gcaaagggaa agtacccatt
     1681 catcatgaag ccggacggcc tgggtgccct cttcgggcct ggtatggtag aaggaccatt
     1741 cccggaacat tacgaacccc tggaaggacc cctgacgaaa aacccccttt cgggtcagct
     1801 gatcaatccc gccattgaga tattcaagag cgatatggat aaggtagcca acgccgacgc
     1861 gaaatttcct tacgtctgca ccacctattc ctgcacggaa cactggtgct ccggtgccct
     1921 cacccggtgg caggcctggt tgctcgagat gcagcccgag ctgtacgtgg agatcgggga
     1981 aaaactggca caggaaaagg gaatccagaa cgggtcgcgg gtgaaggtgc aatctgtccg
     2041 cggccaggtc gaatgcgtcg ccatggtgac gaagcggttc caaccattca cggtcgaggg
     2101 gaagacggtc caccaggtcg gcctgccttt caattacggc tggctcttcc ccaaagacgg
     2161 aggcgacagc acaaacttcc tcacgccgac cgtcggtgac gccaatacgt tctgccccga
     2221 gtacaaggcc tttatggtga acgtggtcaa gttataggag aagagatgat gagcacttcg
     2281 gaagtgataa aactgatcga tacgacacgg tgcaccgcct gccgcggctg ccaggttgcc
     2341 tgcaagcagt ggaatgagct gccgggtctg aggacgaaaa acagggggag ctatcagaac
     2401 ccccccgacc tgggatggaa cacctggacc ttgatccgtt ttcaggaata cgtgaatgaa
     2461 aaggggacct tcaaatggct gttccgcaag gacggttgta tgcactgcac tgacgcgggc
     2521 tgtgtgaagg tttgccccag cggtgccctc tatcatacgg aatacggaac tgtggggata
     2581 aagaaggatc tctgcatcgg ttgcaaggaa tgcaccgcgg cctgcccctt cgaaatcccc
     2641 cggtacaacc gccggacgga taaaatgacg aagtgcgacc tctgcctgac gcggcttcag
     2701 gccaatcaac agccggcctg tgtaaagtcc tgtccgacgg gggcgctcag tatcggtccc
     2761 aaggacaaga tgctcacggc ggcttacgac cgggtcaaag tgctaggcgg agacgccaat
     2821 gtttacgggg ataagttcct gggcggaacc cacgtgctct acgtcctgag cgagaaaccc
     2881 gaggtctacg ataaactgcc taagagtccg tcgattccgc tttctgtgac ggtctggaag
     2941 gactggttga agcccttgac ttggattgcg ggcggcggcg tcatcgccgg gtcgcttctt
     3001 cattatatac tgcacggtcc aaagcggccc taccctgaag tcgatcaggg tgatcatgcg
     3061 gaaaggggga agtgagagat gcctgagaaa gggatgatca aagcgacgga tctttttgag
     3121 agggtcgttc actggatgct tgccgggtcc tgcatactcc tcttcatcac cggtctaggc
     3181 atgatgttcc gggagctcaa cttcatcggc aactttatgg gtggcctggt gggcctaaaa
     3241 tatgtccaca acttcacagg tatatttttt atcgtttccc ttgtcctcgc cgcccggatg
     3301 tggtggcgtg aggcgggcaa gtttgaattt cctgatgata tagagtggat aaaacaggcc
     3361 gggggctatc tctggcgtgt ggaccatgtg gcggaggccg ggaagtacaa cgccggtcag
     3421 aagatgtttt ttctcactgt ggtgggctgc ggcgccctta tgggtgtgtc ggggctgatc
     3481 atgtggtttc ccatgggcct tcccatgggc cttgtgcggt ggatgtatcc gcttcacgcc
     3541 ttcgggttcg tggtcctttt cgccttcttt ttcgtccacc tctacctggg tacgatcggc
     3601 tcgcccggtt cgctaccggc catgctggac ggctgggtga cgcgcgcctg ggcgaagacc
     3661 cagcacccca agtggttgaa ggagatggag gccgcaggga agctcgaggt ttacggggaa
     3721 gaaaagaagg aagcagtgtc tcactgacga cagaaaaccc gcaggcgacc caaaatctgt
     3781 ctgcgggaga atcctttcac gaacgtaaca agcgattcta cagttgacgg attcaggcag
     3841 atgagtgagt gctcgctcat ttagtagagg atttttgttg ttttttatgc taaatataac
     3901 agaagtaaac ttccgtacac aggggatgac catgaagcgc atccttacta aggtgtttgg
     3961 ctcgatcctt gtcgcgcttt tcctgtcatg cccgcttccg tcccaggcct cggaaaacct
     4021 gaaggccgcg gttgcggcca acttcatggc gcccttcaag gagattacag cggccttcga
     4081 ggcggaaact aaggttaaag tggaggcgac gttctcctcg acgggcagtc tctacgccca
     4141 gatcataaac ggagcgccct acgacgtgtt tctggccgcc gacgaggtga gaccgaggat
     4201 actctttggg aagaagttga gcggtgagcc tttcgtctat gcaacgggga ggcttgtcct
     4261 gtggggagcg ggaaagaatt tctgcggccg agcggattgg aaggatgcct tatccgcgga
     4321 ggacatcagg aaaatcgcct tggccaaccc ggaaacggca ccgtacggtg cggccgcacg
     4381 acaggccctg aggcaggtcg gcctctggaa gaaattagag ggccgggttg tcacggcgca
     4441 gaacttgggt caggcgttcc agtacgcctt gatgggtgga gtcgatgcct ctttctgcgc
     4501 gctctcatcg gcgctgactc ccgaggggga aaagggttgt tattacgtcg tggaagaggc
     4561 accgccgatc aggcaggcgg cttgccttct taaaaattct gccaggcaga aggaagcagg
     4621 agagtttttg cgatttcttt cttctcccaa ggccgaggcg atcaagaaaa gatacggtta
     4681 tcattgatgg attttctccc cctctatctg tcggcgaaac ttgcggtgat cacaacgtct
     4741 gttctgatcc ttatatcagc accgcttgca tacgcgctcg tttacgcgaa atggccggga
     4801 aagttccttt tcgaggccct cgtaaacctt cccatcgccc taccgccgac ggtcaccggg
     4861 ctttatctcc tctatctgtt ggggccgcaa gggtatgtgg gagcgatatg gaaggaagtc
     4921 ttcgggggga ctctcctctt cacctttgcc ggtatcgtca tcgcaaccgc cgtttacaac
     4981 cttcctttcg cggttcaacc catcaaggcg acattcgaaa aaatcgaccc ccgtctacgg
     5041 gaaaacgcct atgtgctcgg catctccccc gtggcgactt tcttccgggt catgatcccc
     5101 aactgtatca acgggatcgc ggcggcggcg atccttgtct ttctccatag cctgggcgcc
     5161 ttcggagtcg tactgatggt gggcggaagc atatctggtg aaacgagagt ggcctccatc
     5221 gcgatctatg aagcggtcga gacgatgaac ttcagcgagg cgtggatctt gtccctcgcc
     5281 ttcctgccgg tcagttattt ccttctcctc ctggttaacc gattaacgag gcgttgagtc
     5341 gatgggttta agggcgattt tgcggaaaaa aacggagtat ttctctatcg acgtggactt
     5401 cttctgcccg gaagggaaga ccctcgtcct gatcggcccc tcgggggcgg gcaagacgac
     5461 cataatccgc atgctcgcgg gattggatag gcccgatgac ggaaggatca ccttcagggg
     5521 caaggtgatc ttcgacgcgg accgggatat ctgtctcccc ccccagaagc gggacctggg
     5581 ctatgtcttt caggatttca ccctctttcc tcacctgacg atacacggaa acgcctcttt
     5641 cgcggccaaa gacaaaaatg atgtcgacca tctcataaat tatttcggtc tcgcgcatct
     5701 ccgcgaccgg aaaccccatc aggtatcggg gggtgaaagg cagcgctgcg caatctgcca
     5761 ggccttggca aggcgcccgg acttactcct cctcgacgaa cctttttctg cccttgacgt
     5821 ggtgactcgc cgcggcctac gcgccgaact caaaaaaatc aaagaagaac gcgggctttc
     5881 cgtcgttttt gtaacccacg acatccacga ggccctgttc atcgccaact ccattcttcc
     5941 catcgtcgac ggcaaggtcg accgggattg gcttgagcat atcacgtcgc cgtcgtcgcc
     6001 ccgcgacgca caaccaaggg ccgtccgcga gccgaagctc gccctggttt attgaaaggt
     6061 gagaggtaag atgaagatcg ggacttactc atacgaggaa tacatacacc tgataaaatc
     6121 cttccacggg aacccggctc ccgggctgct catcggcggc ttcatggtcg atgaagccct
     6181 gcgcaggctt cccagcgggg agtttttcga cgccatctgc gaaacctaca cctgcctccc
     6241 cgatgcggtg cagatactca cgccctgcac ggtaggaaac gggtggctca aggtcttcga
     6301 ctacggtcgt ttcgccctgg cgctgtatga aaagtacagc ggtgaaggga ttcgggtcta
     6361 cctcgacccg gccaagctgg acccgtggcc ggagataaag ggatggttcc tcaaactgac
     6421 gcccaaggcg aaccaggaca gtcaaaaact gttcgacgag atcaaagagg cgggcacgaa
     6481 tatcctcagt ttccacaagg tgcggattag gaccgaaaat ctcaaaaaga aaaagatggg
     6541 ccccgtaggc gtctgtccct cctgcggaga ggcctatccg cttcgcgacg gcagcgtctg
     6601 ccgtccttgc cgcggtgaga gcccttatgt caaagaggag tcctgaccgg ccgatcgcct
     6661 ctactcctct cgcccgcact cac
//
LOCUS       DAOTMX010000053         6680 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_9911_length_6680_cov_3.726642, whole genome shotgun sequence.
ACCESSION   DAOTMX010000053 DAOTMX010000000
VERSION     DAOTMX010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 6680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6680
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9911_length_6680_cov_3.726642"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /locus_tag="P5573_02495"
     CDS             complement(<1..805)
                     /locus_tag="P5573_02495"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HRT26968.1"
                     /translation="MLCRTTDDVKKLVEERNVSFVQYWFVDVLGFLKSFSITPSELGE
                     GLTDGMGFDGSSIEGFARIEESDMIAKPDPTTLQFLPWRSGERPSVRMFCDILNPDGT
                     PYDGDARYVLKRMLKRAADMGYTFYVGPEPEYFYFESSDAPRIIDRAGYFDTRPLDKG
                     GDLRRDTIFALQDMGIQIEYSHHEVAPSQHEIDMRYDEGLKMADKLVTLRTVVKEVAR
                     QHGYYATFMPKPIFGENGSGLHCHQSLFKGETNAFYDPKDKYSLSEVGKC"
     gene            1164..2048
                     /gene="pdxS"
                     /locus_tag="P5573_02500"
     CDS             1164..2048
                     /gene="pdxS"
                     /locus_tag="P5573_02500"
                     /inference="COORDINATES: protein motif:HMM:NF003215.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HRT26969.1"
                     /translation="MELFGTFTVRSGLAEMLKGGVIMDVTTPEQAKIAEDAGAVSVMA
                     LERVPADIRAEGGVARMTDPAIIEKIMDAVSIPVMAKVRIGHFVEAQILEAIGVDYID
                     ESEVLTPADDKYHINKWNFKVPFVCGAINLGEALRRIGEGAAMIRTKGEAGTGNVVEA
                     VRHMRTINDEIAGLASLPQEELMTAAKNLGAPYELVKWVAKEKKLPVVNFSAGGIATP
                     ADAAMMMQLGADGVFVGSGIFKSENPAKRAQAIVQAVTHYNDPAILAKVSKSLGGAMP
                     GLEISSIAEGNLLQFRGN"
     gene            2095..3171
                     /locus_tag="P5573_02505"
     CDS             2095..3171
                     /locus_tag="P5573_02505"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF009897.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HRT26970.1"
                     /translation="MKVKITKAAPDKRKPKPTDESKLGFGKIFTDHFFTMKYTTDRGW
                     HDAQIEPYRQLMLDPAAMALHYGQEIFEGLKAYRGADGEIYLFRPADNIRRLNASARR
                     LCMPEVDVDETLEAMKQLIILEKDWIPRGRGTSLYIRPTMIATEPALGVRPSDEYLFY
                     IIVSPVGAYYPQGFSPTKIYVTTDYVRSVVGGIGACKAAANYAASLLAGKEAGEMGYT
                     QVLWLDAIERKYAEEVGTSNIFFLVKDELVTPPLTGSILPGITRDSVIKLARKWGMKV
                     SERQISIDEILSSIDSGAMKEVFASGTAAIVSPVGFIHYKLKEYPINGGAVGPVTEKL
                     YNEILKIQYGEQDDPFGWRLKIAD"
     gene            3433..4143
                     /locus_tag="P5573_02510"
     CDS             3433..4143
                     /locus_tag="P5573_02510"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HRT26971.1"
                     /translation="MALSAIECLKKRRLPKLRETFFIFFLTALILAPHSLRAEVPTLE
                     DLVSRIQENYEKAGDLKAGFIQETTIKTLKKTEREEGVVYLKKPKKMLWDYTRPKAKK
                     LVINPKKAWLYVPDDNIVYVQDAKKVLNSRLLIKFLTGVGRLKEDFQVRYTHPRETDE
                     RGNYLLELVPRQAGLGIDRLLLTIDRDDYRIVSCSLTDLYGNLTKIAFRDLKVNNNLP
                     DSLFTFSPPPGANVQAVP"
     gene            4224..5180
                     /locus_tag="P5573_02515"
     CDS             4224..5180
                     /locus_tag="P5573_02515"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HRT26972.1"
                     /translation="MAVQTRTNDITSRGEAVKGWVQLSRPPFQLAGVLPFVLGAVLAW
                     RMYGTFNWPVFGLGTLAVILIMLSTYYAGEYYDLKGDKLSAERERNRFSGGSQAILKG
                     LVPQKQVKTASYLTLILAAVIGLILQFYYETGPWTIPLGTAGIVAGFFYSTPPLRWVR
                     RGVGELLIGLCYGWLPVAAAFYLQAGTIESIVHWVSIPIGCTIFNVILINEFPDYPAD
                     LTEGKENLVVRLGKNTAAFLYIAMTVTAWVAFGLAMSQGLPVVTVLFYLPFFLISLIL
                     AVLMYKKVYLDRKRLELICGLTIVVNFGTSLSYTLAVWPGGA"
     gene            5187..6410
                     /locus_tag="P5573_02520"
     CDS             5187..6410
                     /locus_tag="P5573_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRT26973.1"
                     /translation="MLKLLFNQPLRGVFWPPTLIAKQLTSVLYFNDLVEWNRFPDWLL
                     RHSFSSLPVAAGSVGMGCIGFPAHPAWEVTGACNLRCIHCHAASNKPAEGELTTDEAK
                     RFIDDLAGVDEFRMLVYTGGEPLVRPDLLELLHHSKKAGLTNVIATNGTLITEEVAFR
                     LKEAGVVGAAVSLDSSQGTVHNYIRNNDNAFDLAMRGIRAVKKAGILLQINVTAMEYN
                     RAGLEELIELADREGSGIMLMYQLVPVGRGSAIEEAALDVTENERLLKSLACKQKNVS
                     TIIEPVAGPQYWPYLMERRGRSNGAWLKLAKRVFHGCSAGRGFVYIKANGDVWPCPFV
                     EMNAGNVREKSFKEIWRRSDVFENLRNREETLKGKCGECKYRTICGGCRGRALAYHGD
                     YLAEDPSCFLHRNRC"
     gene            complement(6482..>6680)
                     /locus_tag="P5573_02525"
     CDS             complement(6482..>6680)
                     /locus_tag="P5573_02525"
                     /inference="COORDINATES: protein motif:HMM:NF022596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HRT26974.1"
                     /translation="ILVAVGVILAVVGLLFMLGGKIPWLGRLPGDFLVKGKNFTFYFP
                     LTTCIIVSIVLSILMYLFFRK"
BASE COUNT         1600 a         1823 c         1839 g         1418 t
ORIGIN      
        1 aacacttccc gacctcggag agactgtatt tatccttcgg gtcgtagaag gcgttggttt
       61 ctcctttgaa cagggactgg tggcaatgga ggccgctgcc gttctcgccg aagatgggct
      121 tcggcatgaa ggtcgcgtaa tagccgtgct ggcgggcgac ttccttgacc acggtcctga
      181 gggtgacgag tttatcagcc atcttcagac cctcgtcgta gcgcatgtcg atctcgtgct
      241 gactcggggc gacctcatga tgactgtatt cgatctggat gcccatgtcc tggagggcaa
      301 atatcgtgtc ccgccgcaga tcacctccct tgtctaaggg acgggtatcg aaataccccg
      361 cccggtcgat gatcctcggg gcatcgctgc tctcgaagta aaaatactcc ggctccggcc
      421 cgacgtaaaa agtgtaaccc atgtcggcgg cccgcttcag catccttttc aggacataac
      481 gggcgtctcc gtcgtagggc gtcccgtcgg ggttgaggat atcacaaaac atgcgaaccg
      541 acggccgctc gccgctcctc cagggcagga attggagtgt cgtcgggtcc ggcttggcga
      601 tcatgtcgct ctcctcgatc cgtgcaaacc cttcgatcga agaaccgtca aaccccatac
      661 cgtcggtcaa cccctcaccc agttcggaag gggtgatgga aaagctcttc aagaaaccca
      721 ggacatccac aaaccagtat tgaacgaaac tgacattcct ttcctccacc aatttcttca
      781 catcatctgt cgttcgacaa agcatctcga cctcctcata atttataggg ataaagccca
      841 agccctggtt tcagacgaac caggcaaagc tctcttttcc gttacccccg gcatgtcatt
      901 atggcttaca attttcgggc cataaataaa ttcctcataa catcaccagg ttatcattct
      961 cttccacttt ccgtctctat gccctcgcac aattccgtac cttttttggt gtgcttccta
     1021 caagtttgta cctgccgggc aaccgccctc gtttttcgtt aattctttac aaatcccgcc
     1081 ccctttgcaa taataatttt aatttgcaat ttaaacgaaa ttgtgaatat tttgtagcag
     1141 gtattcacag gaggtggata aaaatggaac tattcgggac atttaccgtc aggtcgggcc
     1201 tggccgagat gctcaagggt ggcgtgataa tggacgtcac aacgcccgag caggccaaga
     1261 tagccgaaga tgcgggcgct gtctcggtca tggcactgga aagagtcccc gccgatatca
     1321 gggccgaggg cggcgttgcc cggatgaccg accctgcgat catagaaaag atcatggatg
     1381 cggtgagtat cccggtgatg gccaaggtca ggataggtca tttcgtagag gcccagatcc
     1441 tcgaggccat cggggtcgac tacatcgacg aaagcgaggt cctcacaccg gcggacgaca
     1501 aataccacat aaataagtgg aacttcaagg tgccttttgt ttgcggcgcc atcaatctcg
     1561 gcgaggcatt gaggaggatc ggcgaagggg ccgccatgat caggaccaag ggagaagcag
     1621 gtaccggcaa cgtcgtcgag gccgtcagac atatgcggac catcaacgac gagatcgcag
     1681 gactggcgtc ccttccccag gaagagctga tgacggcggc aaaaaacctc ggcgcccctt
     1741 acgaactggt caaatgggtg gccaaggaaa agaagcttcc tgtggtcaat ttttcagccg
     1801 gcggcatcgc gacgccggcg gacgcggcga tgatgatgca gcttggggcg gacggtgttt
     1861 ttgtcggttc cggtatcttc aagtcggaaa accccgctaa gcgtgcccag gctatcgttc
     1921 aggcggtcac ccattacaac gaccccgcta tcctggcaaa ggtctcgaaa tccctgggag
     1981 gtgcgatgcc cggcctggaa atatcgagta tcgccgaggg aaacctgctt cagttcaggg
     2041 ggaattagaa ttcgtcggac gacccgacta tggtggaggg aggaaaatca acagatgaag
     2101 gtcaagatta caaaggcggc cccggacaaa aggaaaccaa aacccactga tgagtccaag
     2161 ctgggtttcg gcaagatatt caccgaccat ttcttcacga tgaaatacac aaccgacaga
     2221 gggtggcacg acgcccagat agaaccctac cggcagctca tgctggaccc ggcggcgatg
     2281 gccctccatt acggccagga gattttcgaa gggctgaagg cctaccgggg ggcggacggg
     2341 gagatctatc tcttccggcc cgccgacaac atcaggcggt tgaacgcctc ggcaaggcgt
     2401 ctctgtatgc ccgaggtgga cgtggacgaa acgttggaag cgatgaaaca actcattatc
     2461 ctcgaaaaag attggatccc ccgcggaagg gggacctcct tgtatatacg tccgaccatg
     2521 atcgccaccg aacctgcctt gggcgtaagg ccttccgatg aatatctttt ttacatcatc
     2581 gtcagccccg tgggggccta ctaccctcag ggcttcagcc ccacgaagat ttacgtgacg
     2641 acggactatg tccgctctgt cgtgggaggc atcggggcct gtaaggccgc cgcaaactac
     2701 gcagcaagcc tgctcgcggg caaggaagcg ggcgagatgg gttataccca ggtcctgtgg
     2761 ctcgacgcga tcgagagaaa gtacgccgag gaggtcggca cgagcaatat cttcttcttg
     2821 gtcaaagacg agctcgtcac tccgcctctc acggggagca tcctgccggg tataacacgc
     2881 gactccgtca tcaaattggc cagaaaatgg gggatgaagg tttcggaacg acagatttcc
     2941 atcgacgaaa tcctcagctc catcgactca ggggccatga aagaggtctt cgcttccggg
     3001 accgcggcca tcgtgtcacc cgtggggttt atccactaca agctcaagga atatccaata
     3061 aatgggggtg ccgtcggccc ggtgacggag aaattataca atgaaatcct gaagatccag
     3121 tatggcgagc aggacgaccc cttcggttgg aggctaaaga tagccgattg agtcccccgc
     3181 aaccgacttt atgagcatct tcgctatctt ccccgctgac atcaacaatg ttgtggatag
     3241 aactgtggat aaaactgtgg atagactgac caaatagtgg attgtagcag acttgcacct
     3301 ttttgcataa cgtttaagca ttgcatatat tgaatgatat catatacata aacaatttaa
     3361 ccctgatctc ggcctgttat cctagtatat cgcccacata cgtgatttat gcgaaaaaca
     3421 taccatcaac ccatggcctt gtcggccata gagtgcctta agaagaggcg gctgcccaag
     3481 ttacgggaaa cctttttcat attttttttg acggccctga tactggcgcc ccattcactc
     3541 cgcgccgagg tcccgacgct cgaagacctc gtctcaagga tacaggaaaa ttacgaaaaa
     3601 gcaggggacc tgaaggccgg tttcatccag gagacgacca tcaagacgct caaaaaaaca
     3661 gaacgggagg aaggggtcgt ctacctcaag aaaccgaaaa agatgctttg ggactacacc
     3721 aggccaaaag caaaaaagct cgtgatcaac ccgaaaaagg cgtggctcta tgtccccgat
     3781 gacaacatag tgtacgtcca ggacgcaaag aaggtcctca attcacgact gctcatcaag
     3841 ttcttgactg gtgtgggccg gttgaaagag gacttccagg tacgttacac ccatccgcgg
     3901 gaaacggacg aaaggggcaa ctaccttctg gagctcgtcc cccgtcaggc tgggctgggg
     3961 attgacagat tgttgctgac gatcgacagg gacgactacc ggatcgtctc ctgctcgttg
     4021 accgacctct acggaaacct gacgaaaatc gccttccggg acctaaaggt caacaacaat
     4081 cttcccgata gcctctttac cttcagtccg cccccgggag caaacgtgca ggctgtcccg
     4141 tgaggtcgtg ctcgatgttt tttaatctgc ttcagtagcc gattgcggta agtttttcat
     4201 atgccacaga ggtaaacccg gaaatggccg tgcaaacaag aaccaatgac atcacgtcga
     4261 ggggcgaagc cgtcaaggga tgggtccagt tgtcgcgacc cccgttccaa ctggccggtg
     4321 tcttgccgtt cgtcttaggt gcggtcttag cctggcgcat gtacgggacg ttcaattggc
     4381 cggtcttcgg cctggggacc ctggccgtga ttcttatcat gctctccacc tattatgccg
     4441 gggaatatta cgacctcaaa ggcgacaaac tctctgccga aagagagagg aaccgctttt
     4501 ccggcggttc ccaggccata ctaaaaggcc tggtaccgca gaagcaggtc aagacggcga
     4561 gttacttgac gctcatcctg gcggcggtaa tcggactcat ccttcaattt tattacgaaa
     4621 ccggcccttg gacgatcccc ctgggaactg ccggcatagt cgccggcttc ttttactcta
     4681 caccgccgct ccgttgggtg agaagaggcg tcggcgaatt gctgatcgga ctatgttacg
     4741 ggtggctccc ggtggccgct gccttctatc tacaggcggg cacgatagaa agcatcgtgc
     4801 actgggtctc catccccatc ggctgcacca tcttcaacgt catcctcata aacgaattcc
     4861 ctgactaccc cgccgacctg accgagggga aggaaaattt agtggtaaga ctggggaaaa
     4921 acactgcagc ttttctctac atcgcgatga cggtcaccgc ctgggttgcc ttcggtctgg
     4981 cgatgagtca gggattgcct gtcgtgacgg tcctcttcta cctcccgttt ttcttgatca
     5041 gcttgatact ggccgtcttg atgtataaga aggtgtacct cgacagaaag aggctggagt
     5101 tgatctgtgg gctgaccata gtagtcaact ttgggacttc actatcttac accctggccg
     5161 tctggccggg aggcgcttga accaagatgc tcaaacttct attcaatcag cctctgagag
     5221 gcgtgttctg gcctccgact ttgatcgcga aacagttgac gagcgtgctc tatttcaatg
     5281 acctggtcga gtggaacagg tttcccgact ggctcctaag gcatagtttc agctccttgc
     5341 ctgtagctgc gggttctgtc ggcatgggtt gcatcggttt tcctgcacat ccggcctggg
     5401 aggtgaccgg cgcctgcaac ctgcgctgta tccactgtca tgccgcgagc aacaaaccgg
     5461 cggaaggcga attgacgaca gacgaggcca agaggttcat agacgacctg gcaggggtgg
     5521 acgaattccg tatgctggtc tataccggcg gtgagcccct ggtgcgcccc gacctgctcg
     5581 agctgctgca ccattccaaa aaggcaggcc ttaccaatgt gatcgccact aacggcacgc
     5641 tgataaccga agaggtcgcc ttcaggctca aagaagccgg cgtcgtcggg gcggcggtca
     5701 gcctcgattc ttcacagggc acagtccaca attacatccg caataacgac aacgccttcg
     5761 acttggccat gcggggcatt cgggcggtaa agaaggcggg cattctgttg cagataaacg
     5821 ttactgccat ggagtacaac cgcgccggcc tggaggagct gatagagtta gccgacagag
     5881 aggggtcggg gatcatgctg atgtatcagt tggtgccggt cggccgaggg agtgcaatcg
     5941 aagaagccgc cctcgacgtc actgagaacg agagattgct gaaatccctg gcctgcaagc
     6001 aaaagaacgt ctccaccatc atcgaaccgg tggccggccc tcaatactgg ccttatctga
     6061 tggagcggcg cggcagatcc aacggcgcct ggctaaaact ggcaaaacgg gtgtttcacg
     6121 gctgttcggc gggcaggggg ttcgtctaca tcaaggcaaa cggcgatgtc tggccctgtc
     6181 cttttgtaga gatgaacgcc ggcaacgtga gagagaaatc tttcaaagag atatggcggc
     6241 ggagcgacgt attcgaaaac ctgaggaaca gagaagagac cctgaaaggc aaatgcgggg
     6301 aatgcaaata ccgaacgata tgcggcgggt gccggggacg cgccctggcc tatcacgggg
     6361 actacctggc cgaagaccca tcctgttttc tccaccgcaa tcgctgttag cctgagggga
     6421 acatcgaacg aggaacagcg ccgtcaggcc ccgactgtta acgatcgcaa gacaatcggg
     6481 actacttgcg gaagaaaagg tacatgagga tgctcaggac gatgctgacg attatgcacg
     6541 tcgttaacgg gaaatagaag gtgaaattct tccctttgac cagaaagtca ccgggcaacc
     6601 ggcccaacca gggtatcttc cctcccagca tgaaaagaag gcccacgacg gcaaggatca
     6661 caccgacagc gaccaggatt
//
LOCUS       DAOTMX010000054         6580 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_10078_length_6580_cov_3.598621, whole genome shotgun sequence.
ACCESSION   DAOTMX010000054 DAOTMX010000000
VERSION     DAOTMX010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 6580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6580
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10078_length_6580_cov_3.598621"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..533
                     /locus_tag="P5573_02530"
     CDS             45..533
                     /locus_tag="P5573_02530"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HRT26975.1"
                     /translation="MAKEEKDEKIVEEGLQPPKKKPLVKWIIIGLSVLILAAGGTVGA
                     MYFTKKNGSKQASAGQSAETALWAMEPFIVNLVDNQGDRYLKVVIELEVSDKNRLVEL
                     NKIKPKLRDNVLDLLTAKSYRELVDIAGKQRLKDEIGIRLNNFLTTGKIVKVYFTEFV
                     VQ"
     gene            573..1556
                     /gene="fliM"
                     /locus_tag="P5573_02535"
     CDS             573..1556
                     /gene="fliM"
                     /locus_tag="P5573_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HRT26976.1"
                     /translation="MDKILSQEEVDALLRGISDGQIETEKPETRDPSGVMVYDLTNQD
                     RIIRGRMPALEMANERFARTFRTSLSALLRKVVGVSCISVDTLKFGEFMKTLPLPTSL
                     HLFRMDPLRGNAFVVIESKLIFVLVDILCGGTGKDPYRVEGREFTAIENRLIKKVVLT
                     ALADMERAWQALIAVNFTYQRSEINPQFAQVVPPADVVVVVSFEVEMEFSSGILSLCI
                     PYATLEPIREKLECGLLSEQKETDREWSRRIFQALNEARVDAVVELGRTQVKTRKVME
                     LKKGDVIVLDNSSADPLDMFVEGILKFKAHPGTYKGNMAVQITKLIGRKEV"
     gene            1561..1995
                     /gene="fliN"
                     /locus_tag="P5573_02540"
     CDS             1561..1995
                     /gene="fliN"
                     /locus_tag="P5573_02540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HRT26977.1"
                     /translation="MEQDELKEKENQGTSGGEDITSDDPWREVLDSMPSAAKTEVGVA
                     AFDEVSKADRKGNETLDIDFILDIPLELSVELGRSRILIGELLQLGQGSVLELSKVAG
                     EQMDVYVNHRLIARGDVVVVNEKFGVRLTDIVSPAERINKLA"
     gene            2006..2404
                     /gene="fliO"
                     /locus_tag="P5573_02545"
     CDS             2006..2404
                     /gene="fliO"
                     /locus_tag="P5573_02545"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HRT26978.1"
                     /translation="MGNVDVLASVVKMISSLAVVLGLMIGAAYVLKRGFQRAGAVPAD
                     EGLIRVISSHYLGPKNSIMVMEVLGSIMVIGISNNQMSLLTTISGSEAVEKLRESRKG
                     SPPSAVTDHLLNYRSKLAALRLLGKEKKNG"
     gene            2397..3179
                     /gene="fliP"
                     /locus_tag="P5573_02550"
     CDS             2397..3179
                     /gene="fliP"
                     /locus_tag="P5573_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417243.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HRT26979.1"
                     /translation="MVRVSRRRIAGLLCVVLIFGIILVVGDAGAESFPIPSVSLQVSN
                     TAKGSDQTAVVIQLLLLLTVLSFAPAILIMLTSFTRIVVVLSLLRHALGTQGMPPNQV
                     IIGLSLFLTFFIMGPVWNQVNTQALQPYFNKQISTEKAIEMATPPLRDFMLRQTREKD
                     LALFIKVSNSNRPEKPSDVGLSVLVPAFVISELRTAFQIGFLIYLPFLVLDMVVASVL
                     LSMGMLMLPPIMVSLPFKLLLFVMVDGWYLIVGSLVQSFSNV"
     gene            3193..3462
                     /gene="fliQ"
                     /locus_tag="P5573_02555"
     CDS             3193..3462
                     /gene="fliQ"
                     /locus_tag="P5573_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HRT26980.1"
                     /translation="MSPDLIVGIAGETIKVTLLVAAPMLLVGLVIGLVVSIFQAVTQI
                     QEMTLVFVPKILAVLIAMLIVLPWMMNILVTYTQNLFSNIPMYIK"
     gene            3480..4256
                     /gene="fliR"
                     /locus_tag="P5573_02560"
     CDS             3480..4256
                     /gene="fliR"
                     /locus_tag="P5573_02560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HRT26981.1"
                     /translation="MPNITVEQAQLFILIFLRVSTIIVLMPILGDASVPVRVKGGLSI
                     LITALLFPIVKIDAAVMGFDVATLFLRMAGEVMVGAVIGFTARLIFAGIQLAGQLVGF
                     QMGFAIVNVIDPANNAQVSIIAEFLYLIAVLLFLSLNGHHMFISAIAESFRLAPPPGF
                     HLTGPLLQGLMTFMRDMFEVALKIGAPVIAMMFFMSVGLGLVARTVPQINVFIVGFPL
                     QIAVGLIGIGLTMPFFVTIVGRCFGNLERGIYGLLRLMSV"
     gene            4290..5366
                     /gene="flhB"
                     /locus_tag="P5573_02565"
     CDS             4290..5366
                     /gene="flhB"
                     /locus_tag="P5573_02565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HRT26982.1"
                     /translation="MAQTEQDQERTEQASPRRREEARKRGQVARSHEVASFAILMACL
                     VYFYFGSAGVLTRMTGMLKRTLGGAGSKSLDADTVQGLLSGLVVESVLIVLPLFLVVV
                     LAGLLANYLQVGFLLSAESIKPAFSKIDPVKGFKRLFSLRAMTELVKNILKITVIGTV
                     VYFTVKGEMEGTFLLPDQSIRGIMVYVGEITFKIILRTSWILVVLAMFDYLYQRWEYE
                     KGLRMTKQEVKDEYRQTEGEPLVKARIRRLQREMARKRMMANVPKADVVITNPDHIAV
                     ALQYEASRMSAPVVVAKGAGFVAEKIREIARKSGVPIVENKPLARVIYKTVDIGKAIP
                     EKLYRAVAEVLAYVYGLTGRARTG"
     gene            5373..>6580
                     /locus_tag="P5573_02570"
     CDS             5373..>6580
                     /locus_tag="P5573_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HRT26983.1"
                     /translation="MAESYAKTEMLPDWTKNTTIVMAGCVIGILLVMMIPIPTIVLDI
                     LLSLNITFSIIVLLLSMYVLKPLDFSIFPSVLLILTLFRLSLNVASTRLVLLHGNEGT
                     SAAGQVIKAFGTFVVGGNYVVGMIVFLVLVLINFIVITKGATRIAEVAARFTLDAMPG
                     KQMSIDADLNAGLITESMARQRRRDIEREADFYGAMDGASKFVRGDAIAGVIIVFVNI
                     VGGLVVGVLQKGMRIDDAAVNYTLLTIGDGLVTQVPALIVSTAAGMLVTRSTSSTNLG
                     AEVAGQVFLQPKAVATAALMLFVFGLIPGMPKISFFTISAVAGAFAFKLFKSVSAAKE
                     EEEEEQPPTTPKESVEALLPLDLMELEVGYGLIHLVDTAQGGELLERIRALRRQLGVE
                     MGIIVPAIHIR"
BASE COUNT         1358 a         1749 c         2045 g         1428 t
ORIGIN      
        1 ggtcgaggtc acattgacat accatgaagg ataagggtgc agagatggcc aaagaagaga
       61 aagacgaaaa aatcgtagaa gagggtctgc agccgccgaa gaaaaagcct ctggtaaagt
      121 ggattatcat cgggttgtcc gtcctgattc tggctgccgg cggtaccgtc ggggcaatgt
      181 attttacgaa aaagaacggt tccaagcagg cgtcggccgg acaatcggcg gagaccgccc
      241 tgtgggcgat ggagcccttc atcgtgaacc tggtcgacaa ccagggggac aggtatctga
      301 aggtcgtgat cgaactcgag gtgtcggaca agaaccgcct cgtggagctc aacaagataa
      361 aacccaagtt gagggacaac gtgctcgacc tcctgacggc gaagtcttac cgggaactcg
      421 tggatattgc ggggaagcag cgtcttaaag acgagatcgg gatacggttg aacaatttcc
      481 tgacgacggg aaagatcgtg aaggtgtatt tcaccgagtt cgtggtccag tagacgagtg
      541 agcgccggaa gctgaggcgc ggttggggtt cgatggacaa gatactttcg caggaagagg
      601 tggatgccct gttgaggggc atctcggacg gacagatcga gacggaaaag ccggaaaccc
      661 gtgacccgtc gggggtcatg gtatacgact tgaccaacca ggacaggatc atccggggca
      721 ggatgccggc cctcgagatg gcaaacgagc ggttcgcccg gactttccgg accagcctat
      781 cggctcttct ccgcaaggtg gtcggcgtca gttgcatctc cgtcgacacc cttaagttcg
      841 gggaattcat gaagaccctt cccctgccga cgagcctgca cctcttcagg atggacccgc
      901 tgcgggggaa cgccttcgtc gtgatcgagt cgaagctcat tttcgttctc gtggacatcc
      961 tttgcggggg tacaggcaag gatccttacc gcgttgaggg cagggagttc acggcgatcg
     1021 agaaccgcct gatcaagaag gttgtgctga cggccctcgc ggatatggaa cgggcctggc
     1081 aggcgctgat agcggtcaat ttcacctatc aacggtcgga aatcaatccg cagttcgcac
     1141 aggtcgttcc gccggccgac gtcgttgtgg tagtcagttt tgaggtagag atggagttct
     1201 cgtccggcat attgtctttg tgtatcccct atgcgaccct ggagccgatc cgcgagaagt
     1261 tggagtgcgg gttgctcagc gaacagaagg agacggacag agaatggtcg agaaggatat
     1321 ttcaagctct caacgaagcg cgggtggatg ccgtcgttga gctcggacgg acacaagtca
     1381 aaacgcgaaa ggtcatggag ttgaaaaagg gggacgtcat cgtccttgat aacagttcgg
     1441 cggatcccct tgacatgttc gttgagggga ttttgaagtt caaggcccat cccggcacat
     1501 acaaggggaa catggccgtc cagataacca aacttatcgg cagaaaagag gtctgacttt
     1561 atggagcagg atgaattgaa ggaaaaggaa aatcaaggga cttcgggagg cgaagacatc
     1621 acctcggacg acccgtggcg ggaggtgctc gactctatgc cgtcagccgc gaagaccgag
     1681 gtcggcgtcg ctgcctttga tgaagtctct aaggcggaca ggaaagggaa cgagactctg
     1741 gacatcgatt ttatcctcga catcccgttg gaactgagcg tggagttggg caggagtagg
     1801 atactgatcg gcgaactcct ccagcttggg caggggtccg tcctcgagtt gtccaaggtc
     1861 gccggagagc agatggacgt atacgtgaac catcgcctta tcgcccgggg ggatgtcgtc
     1921 gtggtgaacg agaaattcgg ggtccgcctg accgacatcg tctcgccggc ggagaggatc
     1981 aacaagcttg cctgaggagg tcgtgatggg caatgtcgac gttcttgcgt cggtcgtcaa
     2041 gatgatttct tcccttgccg tcgtcctggg actgatgatc ggcgccgcct atgtcttgaa
     2101 gaggggattc caacgggcgg gggcggtacc ggccgacgag gggttgatcc gcgtcatttc
     2161 gtcccactac ctcggcccga agaacagcat catggtgatg gaggtactcg gcagcataat
     2221 ggtcatcggc atctctaaca accagatgtc tctactcacc acgatctccg gctccgaggc
     2281 ggtcgagaaa ctgagggagt cgagaaaggg aagcccgccc tccgccgtga cggaccatct
     2341 gctgaattac cggtctaaac tggcggccct gcggctgttg gggaaagaga agaagaatgg
     2401 ttagggtatc ccggcgccgt atcgccggcc tcctatgcgt cgtcctgatt ttcggcatca
     2461 tccttgttgt cggggatgca ggggcggaat cttttccgat cccgtcggtg agcctgcagg
     2521 tatcgaacac ggcaaagggg tcggaccaga cagccgtcgt cattcaactg ctcctgcttc
     2581 ttacggtgct ctcgttcgcg ccggccatcc tgatcatgct tacctctttc acccgcatcg
     2641 tggtcgtgct ctccctcctc agacatgccc tggggacaca gggaatgccg cccaatcagg
     2701 tcatcatcgg cctgtcgctc tttctcacct ttttcataat ggggccggtc tggaatcagg
     2761 tcaacaccca ggcccttcag ccctacttca acaaacagat atccacggag aaggcgatcg
     2821 agatggcgac cccgccgctc agggatttca tgctccggca gacgcgggag aaggacctcg
     2881 ccctcttcat caaggtgtcg aattccaatc ggccggaaaa gccctcggat gtcggcctgt
     2941 cggtcctcgt gccggctttc gtcatcagcg aactgaggac cgccttccag atagggttct
     3001 tgatctacct gccctttctg gtgctggata tggtggtggc gagcgtccta ctttccatgg
     3061 ggatgctgat gttgccgccc atcatggtca gcctgccgtt caagctgctc ctcttcgtca
     3121 tggtggacgg ttggtacttg atagtgggct cccttgtgca gagcttctct aacgtatgag
     3181 agaggttagt ccatgtcccc ggatttgata gtcggcatcg caggagagac gatcaaggtg
     3241 acgctcctag tcgcggcccc gatgctgctc gtggggttgg tgatcggact cgtggtcagt
     3301 atctttcagg ccgtgacgca gatccaggag atgaccctgg tttttgtacc caagatcctt
     3361 gcggttctga tcgccatgct cattgtgttg ccctggatga tgaatatcct ggtcacgtac
     3421 acgcagaacc tgttttccaa cataccgatg tacataaagt aggccaaggg cgagggacca
     3481 tgccgaatat cactgtagag caggcacaat tattcatcct gatctttctg cgggtgagca
     3541 ccataatcgt cctgatgccg atcctcggcg acgcctcggt gcccgtgcgg gtcaagggag
     3601 gtttgtccat actgataacg gcgttgctct ttcccatcgt gaagatagac gcagccgtca
     3661 tggggttcga cgtggcgacc cttttcttga ggatggccgg tgaggtaatg gtgggggcgg
     3721 tgatcgggtt cacggctcgc ctgatcttcg cgggcatcca gcttgccggg cagctcgtgg
     3781 ggtttcagat ggggtttgcg atcgtcaacg ttatcgaccc tgcaaacaac gctcaggttt
     3841 ctatcatcgc ggaatttctc tacctgatcg ccgtcctcct tttcctgagt ctgaacggac
     3901 accacatgtt tatcagcgcc atagccgaga gcttccgtct ggccccccct ccgggttttc
     3961 atctgacggg tccgttgttg cagggcttga tgaccttcat gcgagacatg ttcgaggtgg
     4021 ccttgaagat aggggcaccg gtgatcgcca tgatgttttt tatgagcgtc ggcctcggcc
     4081 tcgtggccag gacggtgccg cagatcaatg tcttcatcgt tgggtttcct ctccagatcg
     4141 ccgtcggcct gatcggtatc ggcctgacaa tgcctttttt cgttacgatc gtcggacgct
     4201 gtttcggtaa cctggagcgg ggtatttacg ggctcttgcg tctgatgagt gtgtgaacgt
     4261 gaacgtagca tcggcttcga gaatgtagaa tggcacagac ggaacaagac caggagagaa
     4321 ccgaacaagc ctcgccgcga cgcagagaag aggcgaggaa aaggggccag gtggcgagga
     4381 gccacgaggt cgcttccttc gccatcctga tggcctgcct cgtctatttc tacttcgggt
     4441 cggcgggggt gctgacgcgg atgaccggga tgctgaagag gaccttgggt ggagcggggt
     4501 cgaagtccct cgacgctgac acggttcagg gattgctttc cgggctggtg gtcgagtccg
     4561 tgctgatcgt tctccccctt ttcctcgtcg tcgtccttgc ggggttactc gcaaactacc
     4621 tccaggtcgg ttttcttctc tccgcggaat cgatcaagcc tgcattttcg aagatcgacc
     4681 ctgtgaaagg attcaaaagg ttgttttccc tgagggcgat gacggaactg gtgaagaaca
     4741 tcttgaagat aaccgtcatc gggacggtcg tctactttac cgtgaagggc gagatggaag
     4801 gtacattcct gctcccggac cagagcatac ggggtatcat ggtttacgtc ggggagataa
     4861 cgttcaagat tatcctcagg acaagctgga ttctcgtcgt cctggcgatg ttcgactatc
     4921 tctatcagcg gtgggaatac gaaaaggggc tccggatgac gaagcaggag gtcaaggacg
     4981 aataccggca gacggagggc gagcccctgg tgaaggcgag gatccgacgc ctgcagaggg
     5041 agatggcgag gaagcggatg atggcgaacg tcccgaaggc cgacgtggtg atcaccaacc
     5101 cggaccatat cgccgtcgcc ctgcagtacg aggcgtcccg gatgtcggca cctgtcgttg
     5161 tcgccaaggg ggctgggttt gtcgccgaga agatcaggga gatcgcgcgg aaaagcggcg
     5221 tccccatcgt ggaaaacaag cccctggcgc gggtcatcta taagaccgtc gacatcggca
     5281 aggccatacc ggaaaagctc taccgggccg tcgccgaagt cctggcttac gtgtacgggt
     5341 tgacgggaag ggcacggacg ggatagtcgc tcatggccga atcatacgca aagactgaga
     5401 tgctcccgga ctggacgaaa aacacgacca tcgtgatggc ggggtgtgtg atcgggattc
     5461 tcctggtcat gatgatcccc atacccacga tcgtactgga catcctgctg tccctgaaca
     5521 tcactttttc gatcatcgtg ttactcctct ctatgtatgt cctgaagccg ctcgactttt
     5581 ccatctttcc ctccgtcctg cttatcctga cgttgttccg tctttcgctg aacgtcgcct
     5641 cgacgcgctt ggtcctgctc catggcaacg aggggacctc cgcggccgga caggtgatca
     5701 aggccttcgg gaccttcgtg gtggggggaa actatgtggt gggaatgatc gtcttcctcg
     5761 tcctcgtgct gatcaatttc atcgtgatca cgaagggcgc cacccggatc gccgaggtgg
     5821 cggccaggtt caccctcgac gccatgccgg gaaaacagat gagcatcgac gcagacctga
     5881 acgccggcct gataacggag tccatggcca ggcagcggcg gcgcgatata gaacgggagg
     5941 ccgactttta cggggccatg gacggggcca gcaaattcgt gaggggtgac gccatcgccg
     6001 gggtcatcat cgtctttgtc aacatcgtgg gcgggttggt cgtcggcgtc cttcagaagg
     6061 ggatgaggat agacgacgcg gcagtgaatt acacgctgct cacgatcggc gacggcctcg
     6121 tgacccaggt cccggcgctt atcgtctcga cggccgcggg aatgctggtc accaggtcga
     6181 cgtcctcgac gaaccttggg gcagaggtag ccggacaggt tttccttcag cccaaggccg
     6241 tcgcgacggc tgcgctcatg ctgttcgtct tcggtttgat cccgggaatg ccgaagatat
     6301 ccttcttcac gatctcagcg gtcgccgggg cctttgcctt caaattgttc aagtctgtga
     6361 gcgccgcgaa agaagaggag gaagaggagc agcccccgac gaccccgaag gagtcggtgg
     6421 aggcgcttct accgctcgac ctgatggagc tcgaggtcgg ttacggcctg atccacctcg
     6481 tcgatacggc gcagggcggc gagctcctgg aaagaataag ggccctgcgt agacagcttg
     6541 gcgtcgagat gggtatcatc gtcccggcaa tccatatccg
//
LOCUS       DAOTMX010000055         6526 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_10190_length_6526_cov_4.556946, whole genome shotgun sequence.
ACCESSION   DAOTMX010000055 DAOTMX010000000
VERSION     DAOTMX010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 6526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6526
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10190_length_6526_cov_4.556946"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="P5573_02575"
     CDS             <1..249
                     /locus_tag="P5573_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT26984.1"
                     /translation="EAACRDVPVIISRQSGVSEVLNHALKVDYWDVQELTDKIIATLL
                     YPALTREMRERCREEARNISWVRTAEKIHAVYRQVCCK"
     gene            459..1037
                     /locus_tag="P5573_02580"
     CDS             459..1037
                     /locus_tag="P5573_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT26985.1"
                     /translation="MPTLKGKSLGFLLVLILLVCLITPSGCAGKKEAVKGPREVLSFY
                     LDALIGGRVEEARQNLASGGKNSARIAAFTPGQSEEENFIRGQLAKKIHYVVKDVILI
                     ENTAEAVVEITAPDYEKVIKDLHANLSWKELPVGSLEAHAFITSAIGKIIKKYQEKGI
                     PMKSGVETFYLVNEAGGWKVDIDGKSRRRGPS"
     gene            1183..1710
                     /locus_tag="P5573_02585"
     CDS             1183..1710
                     /locus_tag="P5573_02585"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HRT26986.1"
                     /translation="MNEDKNRLSTLEVALRNELWERDFYLINAEKTANPAGKAMFRQI
                     ADDELEHYERLKQLHEHWAGTKKWPETVPLKVRNTTVRDILKDFVTKAENMPKGDADD
                     LKAIRTALDFEAKGEKHYAELRDSVQDEKEKAFFDLLSRMEREHYLSLADLEELMTDP
                     VSWLRRKEHHGMDGA"
     gene            1799..2260
                     /locus_tag="P5573_02590"
     CDS             1799..2260
                     /locus_tag="P5573_02590"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRT26987.1"
                     /translation="MKRTVISNILVAVDGSRVSRKAAIYAVELAKGLNASVTFISVID
                     NTAYTGRTAIPAAFTPTRITEPVEDYLRRAAEAFTSRLEKACQERGVTARSVIRVGSP
                     VEEILKEGRRSKADLIVAGSHGRSAAGAALLGSVAFGIVHRSQKTPVLIVR"
     gene            2306..2884
                     /locus_tag="P5573_02595"
     CDS             2306..2884
                     /locus_tag="P5573_02595"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HRT26988.1"
                     /translation="MLESFVSTYGYLAVLIGTFLEGETILMLGGFAAKQGYLSLPWVI
                     AAAFAGSTCGDQFFFFLGRRHKEFMLTRRPYWKVQIDKAHRLFERYHALLILASRFLY
                     GFRIVIPYVIGMSHVPVRYYVSLNLVGALVWAVLGGVLGYLFGNVLEILLGDVKEYEV
                     LVMVSVIALWFLLWCSYLIYRRCKGNRPDGRA"
     gene            2959..4776
                     /locus_tag="P5573_02600"
     CDS             2959..4776
                     /locus_tag="P5573_02600"
                     /inference="COORDINATES: protein motif:HMM:NF014731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HRT26989.1"
                     /translation="MKPDQSDSFWGGIIKAMGLVFGDIGTSPIYTLTVVFALTKPTPD
                     NVFGVLSLVVWTMTILVTVEYVWLAMSLDHKGQGGAIVLREILFRVAKSNRVLSFGTF
                     ITFLDVSLLMGDGVITPAITILSAVEGITLIPGLESVSQSTLIVTAITIAVALFVFQP
                     KGADRIAWTFGPIMFVWFSTLALSGAVSVLKMPELLKAINPYYAIKFFHDNGVAAFFV
                     LSEVILCATGSEALYADMGHLGKKPIIRAWYLVFTALLINYIGQGVFAIHNPETKNIL
                     FGMVQHQVPFLYIPFLILTVMATIIASQAMISGVFSVVYQGITTRLMPLMKVKYTSSK
                     IRSQIYIGAVNWFLMVAVILMLLIFKSSDNLAAAYGMAVTGSMTVTGIMMVMIFHHTT
                     RWKVPFAVIVTCIDFLYLFSTFSKFPQGVYWSLIIASIPLAVMLIWTNGQRFLFYRMR
                     PLPIESFLISYEQIYAKGNNIAGTAIFFTRQWDMVPPYVIHCIIGSQIIYEQNVFISI
                     IITDEPFGVATSYRKGIGTGLDVLEVKVGYMEVIDLEAILKQYDIQEKIIFYGVEDIT
                     TSNPVWRIFSVIKKLIPNFVQFYKLPARKLHGVITRIEM"
     gene            4839..6191
                     /locus_tag="P5573_02605"
     CDS             4839..6191
                     /locus_tag="P5573_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT26990.1"
                     /translation="MTDNMLGRHDGSKESEPRKGAQIIDYGLAKSRGVLSDLHFRISE
                     YATSDRFYEEYELALYEYFEQRSVCTGDEEFFPDEFFRDEEEFERFMSWYTLYFITDE
                     QSRTFPSLYLRENRYQLSPFEEEILKSYDNSYLSLYEVQWVEPGRDFLLKDIFSGRTF
                     LVQDPMFARVLCKWDVLYAGLVSSRRSTFLGGFDPVVIPPQLRNFLQKEILDRFDDVR
                     PQYGVLAEFLRINSAEMSALVDKALEAYYEEPFLNNDGDMLCLATLHYRITDPDAFSR
                     KLGASPLFSLKPPPSTVKKTYIWVHPAKDGQRVCDASPLGTLVIEKNRLRAECNSRER
                     AEKLRALLDESFLPHLQYLTTICEDPGRNLTLPAGANDSAYGLQDGLEYDAWLDRENP
                     ALGGMTPREAAVVPGVRERLVDFLKEIENEEDRMARLGLRKDRHPAFPVEKIMKELGL
                     "
     gene            6195..>6526
                     /locus_tag="P5573_02610"
     CDS             6195..>6526
                     /locus_tag="P5573_02610"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT26991.1"
                     /translation="MSLSLFRKKGVQEVVSHYENNTVKEKKTYVDGRLHGDYVSYYDN
                     GQVMLKLHYEKGLKEGEAVSYYKDGRIREKGTFHKDKLEGEYLSWYENGQLKEREFYK
                     DGKPDGPY"
BASE COUNT         1569 a         1778 c         1771 g         1408 t
ORIGIN      
        1 gaggcggctt gccgtgacgt ccccgtgatc atctcccgcc aatccggggt gtcggaggtg
       61 ctgaatcacg cccttaaggt ggactactgg gatgtacagg aactcaccga caagatcatc
      121 gctaccctcc tctaccccgc cctgacaagg gagatgagag agcgctgccg cgaggaggcg
      181 agaaacatca gttgggtgag gacggcggaa aaaatccacg cagtctatcg ccaggtctgc
      241 tgtaagtgac aagacatccc taatgctgaa acatacatct tgcggcattg catggtcata
      301 gcaacaggag accaaaatcg acgacctttt gccgtatttt ctactttcct tgactgcatt
      361 cgcctttctg atgtatacgt tcacaggttt tctttctcga caaagctcac gggaagaaaa
      421 aggggaagaa taattcactg gcgacacccg cttccggtat gccgacactt aaaggaaaat
      481 ctctcggttt tctcttagtc ctcattctgt tagtgtgcct tattacaccg tcaggatgcg
      541 cgggaaagaa agaagccgtt aaaggccccc gggaagtcct ttctttttat cttgatgccc
      601 ttatcggcgg cagggtcgag gaagcccgtc agaaccttgc ctccgggggt aagaattccg
      661 caaggattgc ggcctttact cccggacagt cggaggaaga aaacttcatc cgcggtcagc
      721 tcgcaaaaaa aatccactac gtcgtcaaag acgtcattct catagaaaac accgcggagg
      781 cggttgttga gatcacggct cccgactatg aaaaggtgat caaagacctc cacgcaaacc
      841 tgtcctggaa agagcttcct gtgggaagcc tggaggcaca cgccttcata acctctgcta
      901 taggaaagat cataaagaaa taccaggaga aagggattcc gatgaagagc ggggtcgaga
      961 ccttctatct cgtcaatgag gcgggagggt ggaaggtcga tatcgacggg aaatcgagaa
     1021 ggcgggggcc gagttgaaag ttgcgggacc tcgggtgaca agaaggcaga aagacgtctc
     1081 caggcttttg agagaaaaga gacgggtaca ggcagacaag gtaaaacccg acagaaaaaa
     1141 gctgcgtaga ggccgggcgg gactaccaac gaggagggga tcatgaatga agacaagaac
     1201 cgattgagca cactggaagt tgccctcagg aacgaattgt gggaacgaga cttctatctg
     1261 atcaatgccg aaaagacggc taatccggcg gggaaggcca tgtttcgcca gatcgccgac
     1321 gacgaacttg aacattacga aaggctgaaa caacttcacg agcactgggc agggacgaag
     1381 aagtggcctg agacagttcc gctgaaagta aggaacacga cggtccgcga tatactgaag
     1441 gattttgtca caaaggcgga aaatatgccg aagggcgacg cggatgacct gaaagcaatc
     1501 cgtacggcgc tcgatttcga ggcgaaggga gaaaaacact atgccgaact ccgggatagc
     1561 gtccaggacg agaaggaaaa ggccttcttc gacctccttt ccaggatgga gcgggagcat
     1621 tacctttccc tcgcggacct cgaagaactc atgacggacc ctgtttcctg gctcaggcgg
     1681 aaggaacacc acggcatgga tggggcgtaa gttaggttca agatccggga tacagagttt
     1741 cggattaaac aagaaaagtt taccctgacg gacagagcgg taggggaggg aaaacaagat
     1801 gaaaaggaca gtgatcagca acatcctcgt tgccgtggac ggctcccggg tttcgcggaa
     1861 ggccgcaatc tatgccgttg aactcgcgaa ggggctgaac gcctccgtta cctttatctc
     1921 cgtgatcgac aatacggctt acaccggacg cacggcgatc cccgccgctt tcacaccgac
     1981 acgcatcaca gaacccgtcg aagattacct ccgccgtgcg gcggaggcct tcacatcacg
     2041 gctggagaaa gcctgccagg aaaggggagt caccgcaaga agcgtcatca gggtcggctc
     2101 ccccgtcgaa gagatcctca aggaaggtcg caggtcgaag gccgacctca tcgtggcggg
     2161 ctctcacggc aggagcgccg ccggggccgc cctcctgggc agcgtcgctt ttgggatcgt
     2221 tcacagaagc cagaagaccc ccgttctcat cgtcaggtag ctcgggttta acgagagaaa
     2281 agcaaaacaa tggtcttctg attctatgct tgaatcattc gtctctactt acggatacct
     2341 tgccgtcctc atcggcacct ttttggaagg cgagactatc ctgatgctgg ggggattcgc
     2401 cgccaaacaa ggttaccttt cactgccctg ggtcatcgcc gcggcctttg ccggaagtac
     2461 gtgcggcgat caattcttct tcttcttagg ccgtcgtcac aaagaattca tgttgacccg
     2521 acggccttac tggaaagtac agatcgacaa ggcccatcga cttttcgaac gttatcatgc
     2581 cctgctcatc ctggcgtcga gattcctgta tggattccgc atcgtcatcc cctacgttat
     2641 cggcatgagc catgtgcccg ttcgctatta tgtctcgttg aacctggtcg gggccttggt
     2701 ctgggccgtc ttagggggcg ttctggggta tcttttcgga aatgtcctcg aaatcctctt
     2761 gggcgacgtg aaggaatacg aggtgctggt catggtctcg gtgatcgccc tctggttcct
     2821 cctctggtgt tcctacctta tctatcgccg gtgtaaggga aacagaccgg acggtcgggc
     2881 ttgattttcc ttattcgata ctggtataag cgtcctcccg gtggtgattg acagcatgag
     2941 gtccgctgga gataaagctt gaagcccgat cagagcgatt ccttttgggg cggtatcatc
     3001 aaggcgatgg ggttggtctt cggagacatc gggacgagcc ccatctatac gcttaccgtc
     3061 gtcttcgccc tcacgaaacc cactcccgac aatgtcttcg gcgtcctgtc gctcgtggtc
     3121 tggaccatga ccatcctcgt gaccgtcgaa tatgtctggc tggcgatgag cctcgaccac
     3181 aaaggccagg gcggcgccat cgtgctgcgc gaaatcctct tccgcgtcgc caaatcgaac
     3241 cgggtgctct ccttcggcac cttcatcacc ttcctcgacg tgtccctgct gatgggcgac
     3301 ggagtcatca ccccggccat caccatcctg tcggccgtcg aaggaatcac cctgatcccg
     3361 gggctggagt cggtcagtca gagtacgctc atcgtgaccg ccatcacgat tgccgtcgcc
     3421 ctgttcgtct ttcaaccgaa gggggccgac aggatcgcct ggaccttcgg tccgatcatg
     3481 ttcgtatggt tctcgacgct ggccctgtcg ggtgccgtct ccgtgctgaa gatgccggag
     3541 ttgctcaaag cgatcaaccc ttactatgcg ataaagttct tccatgacaa cggcgtcgcc
     3601 gcctttttcg ttctctccga ggtcatcctc tgcgcaacgg gaagtgaagc cctctacgcc
     3661 gacatgggac acctgggcaa aaaaccgatc atccgtgcct ggtacttggt cttcaccgcc
     3721 cttctgataa actacatcgg acaaggagtt tttgccatcc acaatcccga aacgaagaac
     3781 atccttttcg gcatggtaca acaccaagtg ccctttctgt acatcccctt cttgatcctg
     3841 acggtcatgg ccaccataat cgcctctcag gcgatgatca gcggggtttt ttccgtcgtc
     3901 tatcagggaa tcaccacgcg gctcatgccc ctcatgaagg tcaagtacac gtcgagcaag
     3961 atccggtcgc agatttacat cggcgccgtg aactggtttc ttatggtagc cgtcatcctt
     4021 atgctgctca tcttcaagag ttcggacaac ctcgcggcgg cttacggcat ggccgtgacc
     4081 ggctcaatga cggtcaccgg gatcatgatg gtcatgatat tccaccacac aaccagatgg
     4141 aaggttcctt ttgcggtcat cgtgacctgt atcgactttt tgtacctctt ttccaccttc
     4201 agcaagtttc cgcagggcgt atactggtcg ctcatcatcg cctccattcc tttagccgtc
     4261 atgttgatct ggacgaacgg gcaacgtttc ctgttctatc ggatgcggcc gttgcccatc
     4321 gagtcgttcc tcataagtta tgagcagatt tacgcaaagg ggaacaatat cgccggtacc
     4381 gccatcttct ttacccgtca gtgggatatg gtgccgccct atgtcatcca ttgcatcatc
     4441 ggcagtcaga tcatctatga gcagaacgtg ttcatttcca tcatcatcac cgacgagccc
     4501 ttcggggtcg ccacttccta ccgaaaagga atcggcacgg gactggacgt actggaagtc
     4561 aaggtcggtt acatggaggt gatcgacctc gaggcgatcc tcaaacagta cgacatccag
     4621 gaaaaaataa tcttctacgg ggtggaggac atcacgactt cgaacccggt atggcgcatc
     4681 ttcagtgtca tcaagaagct gatcccgaac ttcgtccagt tctacaaact ccccgcgcgc
     4741 aaacttcacg gagtcatcac gagaattgag atgtaaggcc tcgcgttttt tctcgacttg
     4801 cccgacgaaa tgggatactc tttctttaac aggagggaat gacagacaat atgctaggcc
     4861 gacatgacgg cagtaaggaa agcgaacccc ggaagggggc gcagatcatc gattatggcc
     4921 tcgccaagag caggggggtt ctctcggacc tccacttcag gatatccgag tacgccacgt
     4981 cggaccgatt ctacgaagag tatgagcttg ccctctacga gtatttcgag caacggagcg
     5041 tctgcaccgg cgacgaagag ttcttccccg acgaattctt ccgggatgag gaagaattcg
     5101 aacgtttcat gtcctggtac accctctatt tcatcacgga cgaacagagc aggacctttc
     5161 cgtcgctcta tctgagggaa aacaggtacc aactctcgcc gtttgaagag gagattctca
     5221 agagttacga caattcttat ctctccctgt acgaagttca atgggttgag ccgggcaggg
     5281 attttctgtt gaaggacatt ttctccgggc ggaccttcct cgtccaggac ccaatgtttg
     5341 ccagggtact ctgcaaatgg gacgtcctct acgccggcct cgtaagcagt agacggtcga
     5401 ccttcctggg ggggttcgac cccgtcgtca tcccgccaca gcttcgcaat ttcctccaga
     5461 aagaaatcct cgaccgcttc gatgatgtgc ggccgcagta cggcgtcctc gcggagttcc
     5521 tgaggatcaa ttcagccgag atgagcgcct tggtcgataa ggcactggaa gcctactatg
     5581 aagagccgtt tctcaacaat gacggtgaca tgctgtgtct ggcgacgctc cattaccgaa
     5641 tcaccgaccc cgatgccttt tcaagaaagc tgggcgcatc tccgctcttc agcctgaagc
     5701 cacctccatc gacggtgaaa aaaacataca tatgggtcca cccggcgaaa gatgggcaaa
     5761 gagtttgcga tgcgtcgccc ctgggaacct tggtgatcga gaagaacagg ctcagggccg
     5821 agtgcaactc gcgggaacgg gcggaaaagc tgagggcgct cctggacgaa tcgttccttc
     5881 ctcacctaca gtacctgacg acgatctgcg aggaccccgg caggaaccta actctcccgg
     5941 caggcgccaa tgacagcgcg tacggattac aagacggttt agagtatgac gcctggttgg
     6001 acagggaaaa tcccgccctc ggcggtatga caccccgcga agcggccgtg gtccctgggg
     6061 tacgggaacg tcttgtcgac ttcctcaagg aaatagagaa cgaagaagac agaatggccc
     6121 gtctcggcct caggaaagac cggcaccccg ccttcccagt cgagaaaatc atgaaggaac
     6181 tgggattgta aaaaatgtcc ctttcgctct tcagaaaaaa aggtgtccag gaggtcgtca
     6241 gccactacga aaacaacacc gtcaaggaaa agaaaaccta tgtggacgga agactacacg
     6301 gcgattacgt gtcttactac gataacggcc aggtgatgct taagttgcat tacgagaaag
     6361 gcttgaaaga aggggaagcg gtcagctact acaaggacgg ccggatacgg gaaaaaggga
     6421 ccttccacaa ggacaagctg gagggcgaat acctttcctg gtatgagaac ggtcaactca
     6481 aggaacgcga attttacaaa gacgggaaac cggacggtcc ttacat
//
LOCUS       DAOTMX010000056         6462 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_10319_length_6462_cov_4.324489, whole genome shotgun sequence.
ACCESSION   DAOTMX010000056 DAOTMX010000000
VERSION     DAOTMX010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 6462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6462
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10319_length_6462_cov_4.324489"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1713)
                     /gene="gyrB"
                     /locus_tag="P5573_02615"
     CDS             complement(<1..1713)
                     /gene="gyrB"
                     /locus_tag="P5573_02615"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HRT26992.1"
                     /translation="MTENSYSADSIKVLEGLEAVRKRPAMYIGSTGKEGLHHLVYEVV
                     DNSVDEATAGFCDTIDVRIRADNSIMVDDNGRGIPVDIHKDEGISAATVALTKLHAGA
                     KFSNESYKISGGLHGVGVSVVNALSEYLELEVRRDGKVYRQSFKRGVPTSDLEVVGKT
                     RGRGTKVVFKPDYEIFEDLDFSFDILSNRLRELAFLNRGIRITLTDDRDGKKSEFFYK
                     GGIVSFVEYINRNKGVLHKKPIYFSGAREDCEVEVALQYNDTYTESIFSFANSINTTE
                     GGTHLSGFKSALTRAINNYAVSSNLIKNGKDSIRGEDVREGLSCVISVKLKSPQFEGQ
                     TKTKLGNSEVKGLVENIVYEKLGSYLEENPTVARQIIGKALDAARARDAARRAKELTR
                     RKGALEGGSLPGKLADCQERDPARSEVYLVEGDSAGGSAKQGRDRRNQAILPLRGKVL
                     NVEKARFDKMLQNEEIKTVVTALGTGIGRDDFDVSKLRYHKVIIMTDADIDGLHIRTL
                     LLTFFFRQMRELIERGHLYIAQPPLFKVVDKKKEMYIHTEEEMKNYILENGVGKMSLV
                     TGNGT"
     gene            complement(1868..2992)
                     /gene="dnaN"
                     /locus_tag="P5573_02620"
     CDS             complement(1868..2992)
                     /gene="dnaN"
                     /locus_tag="P5573_02620"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HRT26993.1"
                     /translation="MEFNVDREVLLDGIQKTLSIVERKTTMPILNNVLLRAGDRGLGI
                     IATNREIGLIAHYDAQVVTEGEITLSARKLFEMIREIQEKVVNFKMNDANWVNVSCGK
                     VSYRIPGISAAEYPKVEEGEGLMFVNMKSSVLGDMISKSAFAMSTDEIRTSLNGIFFE
                     LIEEEDGTRKFRMVATDGSRMSLVDKDTGGQELLSLGKGIIIPRRGVSEIRRLLDEGD
                     EDVAVGVDKGMLIMKKKNTILKVGLIDAEFPNYRRVIDSIEKGTAVTLNREEFLHALR
                     RMAVMASDKYNGVRIKISNGKMLLDSTNPDVGEANEELDVPGSETELDVVYNVRYLVD
                     AVDVVDDENVVFEIREGIKPGTISPARGGNYLSIIMPLCM"
     gene            complement(3144..4508)
                     /gene="dnaA"
                     /locus_tag="P5573_02625"
     CDS             complement(3144..4508)
                     /gene="dnaA"
                     /locus_tag="P5573_02625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HRT26994.1"
                     /translation="MREIWNKSIDIIKKNVTQQNFETWIQPLEIVSLQENNAYLSVPN
                     KFFKDWLSENYTPVIKDALSSVTGFPVQVVFVVDKDSDGAHSHTTTQRERVEHSTKKQ
                     TPKKTLGLKVYTSLNLNYSFERFVVGPCNQFAHAACMAVAEQPAQTYNPLFIYGGVGL
                     GKTHLLNAIGLQALKINPERHVLYVSAEEFMNELIGCIRYDKMPMFREKFRNIEYLLI
                     DDIQFIAGKDRTQEEFFHTFNTLHDSGKQIVVTSDKFPKDIPNLEGRLRSRFEWGLVA
                     DIQPPEIETKMAILIKKAEENQLNLPNNVAYFIASKSESSIRELEGYLIRIAAYASLS
                     GKEITMDLAKEILNDILKQGAPVEVSVDEIIKTVAGKFAIKINDLKSEKKNKNLVIPR
                     QITMYLARKLTSDSFPDIGAKMGGKDHSTVIYANNKIKKLVDTDMKIKKIVEELEDIL
                     LKKG"
     gene            complement(4681..5553)
                     /locus_tag="P5573_02630"
     CDS             complement(4681..5553)
                     /locus_tag="P5573_02630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRT26995.1"
                     /translation="MMPPKTTLGKGLGAIFPDILENIEERKPFILCGIEELIPNRFQA
                     RKVFDSKDLEELAVSIRESGIIQPIVARKAEGGYEIIAGERRWRAAQAAGLKEVPIIV
                     REATDQDVALVSVVENLQRADLNPIEEGTAYYNLMNSFGLSQEEVAEKVGKDRSTVAN
                     MVRLLRLPQDIKDALATKKLSAGHARVLLSLNTESEQVEVYNLVRKKGLTVRETEVLV
                     RRFKSSPRPATVKAKKNEILDLEEKLSKALMTRVLVKPAKKGGSIEIRFHSSKDLDRL
                     VGLILEKGEALNHL"
     gene            complement(5550..6320)
                     /locus_tag="P5573_02635"
     CDS             complement(5550..6320)
                     /locus_tag="P5573_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRT26996.1"
                     /translation="MRKTICIANQKGGVGKTTTAINLAASLAAAEKKTLLVDCDPQAN
                     ASSGLGVDRELAGKTNLYHALIGEVPLDDVIVGAPIPTLEIAPSSQDLIGVEVEFVSL
                     EDREKRLRNALRAMNRHYDFVIIDCPPSLGLLTVNALVAADDVIVPLQCEYFAMEGLG
                     HLLNTIRLVRARLNPPLSLSGILLTMFDTRNRLSHLVAEDVRKHFGDKVFKTIIQRNV
                     RLSESPSHGLPVMLYDIKSRGAVAYLELAKEILSNGRA"
BASE COUNT         1326 a         1773 c         1627 g         1736 t
ORIGIN      
        1 cgtcccatta cccgtcacga ggctcatctt gccaacgccg ttctccagga tgtagttctt
       61 catttcttcc tcggtgtgga tgtacatttc cttcttctta tccaccacct tgaagagggg
      121 cggctgggcg atgtagaggt gaccgcgctc gatcagctcc cgcatctggc ggaagaagaa
      181 ggtgaggaga agcgtgcgga tgtggaggcc gtcgatgtcc gcatcggtca tgatgatcac
      241 cttgtggtac cggagcttgc tcacgtcgaa atcatctctg ccgataccgg ttccgagcgc
      301 tgtgacaacg gtctttatct cctcgttctg cagcatctta tcgaagcgtg ccttctcgac
      361 gttgaggacc ttgcccctga gcggcagtat cgcctggttt ctccggtcac gcccctgctt
      421 ggccgagccg ccggcgctgt cgccctccac caggtacacc tcgctgcggg ccgggtcgcg
      481 ctcctgacaa tcggcgagct ttcccggaag cgagccccct tcgagggcgc ctttccggcg
      541 cgtcagctcc ttagcccttc gggcggcgtc ccttgccctt gcagcgtcga gggcctttcc
      601 gattatctgt ctcgccaccg tcgggttttc ctcgaggtaa ctgccaagct tctcatagac
      661 tatgttctcg acaaggccct tgacctcgct gtttccgagc ttggtcttcg tctgaccctc
      721 gaactggggg gacttgagtt tcacgctgat gacgcaggag agcccctcac gaacgtcctc
      781 accgcggatc gagtccttgc cgtttttgat gaggttgcta ctcacggcgt aattattgat
      841 ggcgcgggtc aatgccgatt tgaacccgct caggtgcgtc cctccttccg tcgtgttgat
      901 gctgttggcg aaggaaaaga tgctttccgt gtacgtgtcg ttgtactgca aggccacctc
      961 tacctcgcag tcctcccgcg ccccgctgaa gtagatgggc ttcttgtgga ggacgccttt
     1021 gttgcggttt atgtactcga caaaagagac gatcccgcct ttgtagaaga attcgctctt
     1081 ctttccgtcc cggtcgtcgg taagcgtgat cctgatacct ctattcaaga aggcgagttc
     1141 ccgcaggcgg ttggaaagga tgtcgaaact gaagtccaga tcctcaaata tctcatagtc
     1201 cggcttgaat acgacctttg tccccctccc ccgggttttc ccgaccacct caaggtcgga
     1261 tgtcgggaca cccctcttga aggactgtct gtacaccttg ccgtccctgc ggacctccag
     1321 ttcgaggtac tccgaaaggg cgttgacaac agaaacgcct acgccgtgca atcctccgga
     1381 aatcttataa ctttcgttgg aaaacttggc gcccgcatga agcttggtca gggccaccgt
     1441 ggcggcagag atgccctcgt ctttgtggat gtctaccggt atccccctgc cgttatcgtc
     1501 aaccatgatg ctgttgtcgg cccggatgcg cacatcgatg gtgtcacaga agccggcagt
     1561 cgcctcgtcg acactgttgt ccacgacctc atagacgagg tgatggaggc cttctttccc
     1621 cgtactgccg atgtacatgg ccggtcgctt gcggacggcc tcgagaccct cgagaacttt
     1681 tatgctgtca gccgaataac tgttttctgt cataatgcct ttccaaatat ccacacacca
     1741 gtaatttatc catacaaaac ccgagtaagt aacaccgggg tccaaatcaa ttataccata
     1801 tcatacctga cggcatgggg gtaaaaatag acttttctca gcgtaatatc gatgatgatc
     1861 aacaatttca catgcatagc ggcatgatga tagagaggta attcccacct cgcgccggac
     1921 tgatcgtccc cggttttatc ccctctcgta tctcgaagac gacattttcg tcatcaacta
     1981 cgtcgacggc gtcgaccaga taccggacat tgtaaactac atccaattcc gtctccgaac
     2041 cggggacatc caactcttcg ttggcctccc ccacatcggg gttggtcgag tctaagagca
     2101 tctttccgtt ggatatcttt atccggaccc cgttgtactt atcgcttgcc atgaccgcca
     2161 tcctcctcag ggcatggagg aactcttcac gattcaaggt aacggccgtc cctttctcga
     2221 tactgtcgat tactctcctg tagttgggaa attctgcatc gatcaggccg accttgagaa
     2281 tcgtgttttt ctttttcatg atcagcatac ccttatccac gccgaccgcc acatcttcat
     2341 ctccctcgtc caacaacctc ctgatctcac tcacacccct cctggggatg attattccct
     2401 tcccgagtga gagcaactcc tgccccccgg tgtccttatc gaccagcgac atacgactac
     2461 catccgtggc caccatccgg aatttcctcg tcccatcttc ctcttctatg agctcaaaaa
     2521 aaatgccgtt gaggctggtt ctgatttcgt cggtggacat ggcaaaagcg gacttactta
     2581 tcatgtcccc gagtacggaa ctcttcatat tcacaaacat gagaccctcg ccctcctcaa
     2641 ctttaggata ttcagcggcg gagatacccg ggatacgata actgaccttc ccacagctta
     2701 cgttcaccca gttcgcatcg ttcatcttga agtttacgac cttttcttga atttctctta
     2761 tcatttcaaa tagtttacgg gctgacaggg tgatctcacc ttctgtgacc acctgggcat
     2821 cgtaatgggc aatcagccct atctccctgt tcgttgcgat gatgcccagc cccctatcac
     2881 ccgcccggag cagcacattg ttcaggatgg gcatcgttgt tttcctttcc acgatactga
     2941 gggtcttttg gatgccgtcc aacaatacct ctcggtcaac attgaattcc atctctactc
     3001 cttacatttc ttagattaga ttaaattaaa tcagtatgga taataatagt acctgtggaa
     3061 ttgttgataa cttgctacgc cgcgaatagc aataaagatt ccgtagaacg cgcctgttga
     3121 tgggttgtcg ttttttgtgg gtatcagccc ttttttaaga ggatatcttc cagttcctcg
     3181 actatttttt ttatcttcat gtcggtatca acaagttttt taatcttgtt gttggcatat
     3241 atgactgtcg agtggtcttt cccccccatc ttggcgccga tatcgggaaa ggaatcgctt
     3301 gtcagttttc gtgccaggta catcgttatc tgcctgggaa tgacgaggtt tttatttttc
     3361 ttttccgatt ttaagtcgtt gattttaatc gcgaattttc ctgccaccgt ctttatgatt
     3421 tcatcgaccg atacctcgac aggcgctccc tgcttgagaa tgtcgttgag gatttctttt
     3481 gcaaggtcca tagttatttc cttgccggac agtgaggcat aggcggcgat acggatcagg
     3541 tatccctcca gttcacggat actcgactca gatttagagg caataaaata agcgacatta
     3601 ttcggcaggt taagctgatt ttcttcggct tttttgatga ggatcgccat cttggtttct
     3661 atctccggtg gttggatgtc cgccaccaaa ccccattcga accgggaccg gagcctcccc
     3721 tcgaggttcg ggatgtcttt aggaaatttg tcgctcgtca ccacgatctg ttttcccgaa
     3781 tcgtggagcg tgttgaaggt atggaagaat tcttcctgag tccggtcctt cccggcgata
     3841 aactggatgt cgtctatgag caagtattcg atatttctga atttttctct aaacatgggc
     3901 atcttgtcat accttatgca cccgatgagc tcattcatga attcctccgc cgagacgtac
     3961 aggacgtggc gttcagggtt gatcttcagg gcctggaggc cgatggcgtt aagcagatgg
     4021 gtcttaccga ggccgacgcc gccgtagatg aagagggggt tataagtctg ggccggctgt
     4081 tcggcaaccg ccatgcaggc ggcgtgggca aactggttgc acggtccgac gacaaacctc
     4141 tcgaagctgt aattaaggtt tagagaagtg tacaccttca acccaagggt cttcttcgga
     4201 gtctgttttt ttgtgctgtg ctctaccctt tcgcgttgtg ttgtagtgtg ggaatgggcg
     4261 ccgtcggaat ccttatctac tacaaaaaca acctgtacgg ggaaaccggt cacggacgac
     4321 agggcgtcct tgatgacagg tgtgtagttc tcggagagcc aatccttaaa gaacttattc
     4381 ggtacgctca ggtaggcgtt attctcctgc aaggagacta tttcgagcgg ttgaatccac
     4441 gtctcgaagt tctgctgcgt tacatttttc ttaattatat caatgctttt attccaaatt
     4501 tctctcacga tcggctacct atgaacaatg ttatcaacag ctgttgacaa atagagggat
     4561 atcgtcccaa gaagtaaaag actctaacct ataaataagt ttaatatctc tgattgaacg
     4621 gtgaggatac gggtattcct gccaagacgg ggagaaacat aatacaaact acggcaaatt
     4681 tcaaagatga tttaatgcct ctcccttttc cagaatcaac cctaccaacc ggtccaggtc
     4741 cttggaagag tggaaccgga tctcgatact cccccctttt tttgccggct tgacgaggac
     4801 cctggtcatc aaggccttcg aaagtttctc ctcaaggtca agtatctcat tcttctttgc
     4861 ttttacagta gccggtcgtg gtgagctttt gaatctacgg acgaggacct ccgtttcccg
     4921 tacggtgagc cctttttttc gtaccagatt gtacacctcg acctgctctg actccgtatt
     4981 cagggacagg aggacccgtg catggccggc ggagagcttc tttgtggcaa gggcgtcttt
     5041 gatgtcctgc ggtagtctca ggagcctcac catgtttgcc accgtcgagc ggtccttccc
     5101 taccttctcc gcaacctctt cctgggaaag gccgaagctg ttcattaggt tatagtaagc
     5161 cgtgccctct tctatggggt tcaggtccgc ccgctggagg ttttcgacga cggagacgag
     5221 ggcgacatct tggtccgtcg cctccctgac gatgatgggc acttccttga ggcctgctgc
     5281 ctgggcggcc cgccagcggc gctctcccgc gatgatctcg tacccccctt cagccttccg
     5341 ggccacgatt ggctggatga tgccgctttc tctgatggaa acggcgagct cttccaagtc
     5401 cttagagtcg aacaccttcc tggcctggaa tcggttgggg attagttctt ctataccgca
     5461 gaggatgaaa ggttttcttt cttcgatgtt ttcgaggatg tcggggaata tagcacccag
     5521 acccttgccg agagtcgttt tcggaggcat catgccctcc cgttggaaag gatttctttt
     5581 gccagttcga ggtacgcgac tgcccccctc gattttatgt cgtacagcat gaccggcagg
     5641 ccgtggctcg ggctttcgga aagccggacg ttgcgctgga ttatggtctt gaagacctta
     5701 tcgccgaagt gttttctcac gtcctcggcg acgagatggg acaggcggtt ccgcgtgtcg
     5761 aacatggtca agaggatgcc cgacagagag agcgggggat tgagccgcgc cctgaccaag
     5821 cggatcgtgt tcagcaggtg gccgaggcct tccatggcga aatactcgca ctggagcggg
     5881 acgatgacgt cgtccgccgc tacgagggcg ttgaccgtga ggaggcctaa agagggcggg
     5941 cagtcgatga tcacaaagtc ataatgccta ttcatcgcac gcagggcgtt gcgcaacctt
     6001 ttctccctgt cttcgaggga gacgaactcg acctccacgc cgatgaggtc ctggctcgac
     6061 ggggctatct cgagggtcgg gatcggcgcg ccgacgataa cgtcgtcgag cggtacttca
     6121 ccgatcaacg catggtaaag attcgtctta ccggccagtt cgcggtctac gccgagcccg
     6181 ctcgatgcgt ttgcctgggg gtcgcagtct acgagaaggg tctttttttc cgccgccgcg
     6241 agggaggcgg cgaggttgat cgccgtcgtc gtcttcccga cgccaccttt ctgattcgct
     6301 atgcagatgg ttttgcgcat cccatggata aatactaaac acaaaattcc gaatgctaaa
     6361 cagacttcgg taaaaaagct aagaagaaga ggagcgacag gcacctggat tgtttggggt
     6421 ttggaaccaa gtttcaggat tcgttctgcg atttttagca ca
//
LOCUS       DAOTMX010000057         6438 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_10365_length_6438_cov_3.288579, whole genome shotgun sequence.
ACCESSION   DAOTMX010000057 DAOTMX010000000
VERSION     DAOTMX010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 6438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6438
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10365_length_6438_cov_3.288579"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1015)
                     /locus_tag="P5573_02640"
     CDS             complement(<1..1015)
                     /locus_tag="P5573_02640"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRT26997.1"
                     /translation="MTKEVFPTFYELIKKRPFEKVKRFVNASDFKNRVEELRLRVRAA
                     RGEVVPDLVLKGGRVVDVFAGEILDADVAVLGGHIVGVGNDYSGPCVIDCRGKFITPG
                     FIDGHIHIESTLLSPRELATALVPHGTTAVVCDPHEIANVLGAGGVEYILDEGAGLPL
                     DVFVMLPSCVPATHLETAGAELSTEELLKLAGRERVLGLAEMMNYPGVLSGREEVLEK
                     IAAFCELPKDGHAPLLSGKDLNAYIGAGIHSDHECTRESEAREKLRLGMHVMIREGTQ
                     AKNLRALLPVVTPRTVARCSLITDDLHPHDILERGHLNYLMERAVEEGLDPVLAVTMT
                     TLSP"
     gene            complement(1121..1669)
                     /locus_tag="P5573_02645"
     CDS             complement(1121..1669)
                     /locus_tag="P5573_02645"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRT26998.1"
                     /translation="MPKPWELVSSAIDRSYRVFGLRTDKARSPRTGEVHEFFVLEAPS
                     WVNVIPLTADDRVVLVRQYRHGIRAVTLEIPGGLIDDGDTPEEAARRELREETGYEGT
                     ELTLLGAVHPNPAIQNNLCYTYLAEDVRPVGDLRQDDGEDIEVVLHPLSDIPALIRKK
                     EITHALVIAAFYHYFAQRMTPS"
     gene            complement(1686..2276)
                     /locus_tag="P5573_02650"
     CDS             complement(1686..2276)
                     /locus_tag="P5573_02650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HRT26999.1"
                     /translation="MSGDYGELREGQRRGALLLILSVAILYAGKTSGLGDALSPMTFL
                     HHNSPSWGETVVELTGDMPGRGIYFLPRGSKVADLLRIAGVTEDELQNMEVPGRVLKA
                     GASVTLKEGADGGKQIKVGRMANARRYALGMPIGLNSATAADLMMVQGIGEKTAERIL
                     ETRAELGRFKAVDDLLEVKGIGEKKLEKFRRSFYVD"
     gene            complement(2283..3503)
                     /locus_tag="P5573_02655"
     CDS             complement(2283..3503)
                     /locus_tag="P5573_02655"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRT27000.1"
                     /translation="MALVVQKYGGTSVADIERIENVARKVIKAREAKNDLVVVVSAMA
                     GETDRLIRLAHSAAPDPIEREYDVLIASGEQVSVALLSIVLNRMGCKAKSYLAYQVKI
                     ITDGDFTRARILKIDRSLIQEDLRQGYVAVVAGFQGIDRKNNVTTLGRGGSDTSAVAL
                     AAALGADVCEIYTDVDGVYTADPNICDNARLLKKICYEEMLELASAGAKVLHPRSVEL
                     AKKYEVPVYVRSSFNDGAGTLVTKEDKDMEKGVVSGVTYDRDQAKITVIHVPDRPGVA
                     ARLFTPLSDENIPVDMIIQNASIDGFTDLTFTVSRKDIGAVRRLLEISAKEVGAQGLQ
                     FDEHIAKVSIVGVGMRSHAGVASKMFATLAKEGINILMISTSEIKISCAIEAKYTELA
                     VRVLHDAFGLEQES"
     gene            complement(3503..3994)
                     /gene="tsaE"
                     /locus_tag="P5573_02660"
     CDS             complement(3503..3994)
                     /gene="tsaE"
                     /locus_tag="P5573_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HRT27001.1"
                     /translation="MGEDRRSFSLVSKSPDETLRIGKVIGARLGPGDVVALVGELGSG
                     KTVLTQGIAQGIPVDEGYVIASPTFTLINEYPGRYRLYHLDIYRLEGSEDLLPLGYED
                     YFYGDGIVVIEWAEKILDILPEETLFVRLRYLDEERREIEIEGFRRRVDGIEDALKEG
                     GFF"
     gene            complement(4001..4387)
                     /gene="acpS"
                     /locus_tag="P5573_02665"
     CDS             complement(4001..4387)
                     /gene="acpS"
                     /locus_tag="P5573_02665"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HRT27002.1"
                     /translation="MIVGVGIDLVDVGRVEKAIIRWDDRFLVKVFLPGEVEYCKRRPF
                     PPRHFASRFAAKEAFLKALGYGLFAGIALTDVEVFVREGGRPELRFYGRAAEVVEERR
                     VGAANLSLSHDGNYAAALVVLEGRPV"
     gene            complement(4384..5592)
                     /gene="folP"
                     /locus_tag="P5573_02670"
     CDS             complement(4384..5592)
                     /gene="folP"
                     /locus_tag="P5573_02670"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HRT27003.1"
                     /translation="MGPIRCLVLNTEAEAAEVLESIGADAYGVRAMAPKMGHLNLLIK
                     DLPCPTANIMKQEMLSLGGDVAVARGTVACSIEKTDALVMGTRKQVGRFAEKIEKQPF
                     GLSAIAKEIARVLENIGKDAFRLETPRRTIELGKRTLVMGIINVTPDSFSDGGKLVDA
                     GAAVRRGVEMEEEGADILDVGGESSRPGAMTVSEEEELRRVIPVVEGLAARVGIPISV
                     DTAKAKVAKAALEAGAELVNDITALNGDDRMAEVAAELKASVVLMHMRGTPRTMQEGD
                     LSYGDLFSEVIDYLAGSIRLALDAGIDPERIVVDPGIGFGKGLRDNIRLLRGLGEFKV
                     LGRPILVGTSRKAFIGAVTGKDAAERLEGTAATVTAAILNGAHIVRVHDVGFMKRVAA
                     MADAIARGSV"
     gene            complement(5611..>6438)
                     /locus_tag="P5573_02675"
     CDS             complement(5611..>6438)
                     /locus_tag="P5573_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HRT27004.1"
                     /translation="EKIFEVHTRKIPLADDVDFAVLARGTPGFSGADIENLVNEAALA
                     AARANQERVRMCDFEFAKDKVLMGTERKSMIISEEEKRTTAYHESGHAFVARLLPGTD
                     PIHKVTIIPRGRALGITQQLPVDERHTYPKQYLMNALTILLGGRAAEELVLNDFTTGS
                     GNDIERATNLARKMVCEWGMSENMGPLSYGKKEEQIFLGREFATHKDYSEETAEKIDR
                     EISRLVSESYEKAKKLLADNLDKLHRLAAALLEKEVLTAEEIDRIIGDGKVADGQVH"
BASE COUNT         1111 a         2173 c         1762 g         1392 t
ORIGIN      
        1 ccgggctgag ggtggtcatc gtgacggcga gcaccgggtc gagaccttct tccacggccc
       61 tttccatcag atagtttagg tgccctctct ccaggatgtc gtggggatgg aggtcgtcgg
      121 tgatgaggga acagcgggcg accgtccggg gcgtcacgac gggcaggagc gcccgcagat
      181 tcttcgcctg cgtcccttcc cggatcatga cgtgcatccc caggcggagc ttctccctcg
      241 cctcgctctc ccgcgtgcac tcgtggtcgg aatgtatccc ggcgccgatg taggcattga
      301 ggtccttgcc cgacagaaga ggtgcatggc cgtctttcgg cagttcgcag aaggccgcta
      361 tcttctccag gacctcttcc ctgccggaaa ggacgccggg ataattcatc atctcggcga
      421 gccccaggac gcgttcccga ccggctaatt ttaagagttc ttccgtcgaa agctcggcgc
      481 ccgccgtctc gaggtgggtc gccgggacgc aggaaggcag cattacgaag acgtcgagcg
      541 gcaggccggc gccctcgtcg aggatgtact cgacgccccc ggcgccgagg acgttcgcaa
      601 tctcgtgggg gtcgcagacg acggcggtcg tgccgtgggg aacgagggcc gtcgccagct
      661 cccgcggcga aagcagcgtg ctttcgatgt ggatgtggcc gtcgatgaag ccgggcgtga
      721 tgaactttcc gcgacagtcg ataacgcatg gcccggagta gtcattcccc acgccgacga
      781 tatggccgcc taaaacggct acatcggcat caagaatctc acccgcgaag acgtcgacga
      841 cccggcctcc tttcagcacc aggtcgggga ctacctcgcc gcgggcggcg cggacccgca
      901 gtcgcagttc ttccacccgg ttcttgaagt cgcttgcgtt cacaaacctt ttcacttttt
      961 cgaaaggtct tttcttgatt agttcgtaaa aagtcggaaa tacctctttc gtcattccgt
     1021 gcttgacata agcctgcccc gtactccgat acgggggaat ccagtttttt caatatgttc
     1081 tggatgccgg atcgagtccg gcatgacgtt tttggacttt ttacgagggc gtcattcttt
     1141 gcgcgaagta atggtaaaag gcggcgatga cgagggcgtg ggtgatctct ttttttcgga
     1201 tgagcgcggg gatgtccgaa agggggtgca acacgacctc tatgtcttcc ccgtcgtcct
     1261 gtctcaggtc accgacggga cggacgtcct ccgcaaggta ggtgtagcag aggttgttct
     1321 ggatcgccgg gttgggatgg acggcgccaa gtaaggtcaa ctccgtacct tcataacccg
     1381 tctcttccct caattcgcga cgggcggctt cttccggcgt atcgccgtcg tcgatgaggc
     1441 ccccggggat ttcgagagtc accgcgcgga tgccgtggcg gtactgcctg acgaggacca
     1501 ccctgtcgtc cgccgtcaaa gggatgacgt tcacccagga cggcgcctcc agcacgaaga
     1561 attcatggac ctccccggta cggggcgacc gggccttgtc cgtccgcagg ccgaaaacgc
     1621 ggtaagacct gtctattgcg ctcgacacca actcccaggg cttgggcata aagacggccc
     1681 ccttctcaat cgacgtaaaa gctacgcctg aacttctcga gtttcttctc gcctatcccc
     1741 ttgacctcca agaggtcgtc cacggccttg aaacgcccca actcggccct cgtttccagt
     1801 atcctttccg ccgtcttctc acctatcccc tgcaccatca tcaggtcggc ggcggtcgcc
     1861 gaattaaggc ctatcggcat acctagggcg tacctgcggg cattcgccat cctgcctact
     1921 ttgatctgtt tccccccgtc ggccccctct ttcaaggtaa cggaggcgcc cgccttcagg
     1981 acacggccgg ggacctccat gttctgcagc tcatcttccg tcacccccgc tatcctcagg
     2041 aggtcggcaa ccttcgaccc ccgggggagg aaatagatac cccggcccgg catgtctccc
     2101 gtcaattcga ctacggtctc gccccaggag gggctgttgt ggtgcaagaa ggtcatcgga
     2161 gagagggcgt cccccaggcc cgacgtcttc ccagcataga gtatggcgac cgaaaggatg
     2221 agaagcagcg caccccggcg ctgtccttct ctcagttccc cgtagtcacc cgacatgttt
     2281 ccctaacttt cctgttcgag cccgaaggca tcatggagga ccctgacggc caactcggtg
     2341 tacttcgcct cgatggcgca ggaaatcttg atctccgacg tgctgatcat caggatgttg
     2401 atcccttctt tcgccagggt ggcgaacatc ttcgaggcga cgccggcgtg actcctcatc
     2461 ccgacaccga cgatcgacac cttggcgatg tgctcgtcga actggagccc ttgggctccc
     2521 acttctttcg ccgatatttc gagcagcctc ctcacggcac cgatgtcttt tcgcgagacg
     2581 gtgaaggtga ggtctgtgaa gccgtctata ctcgcattct ggatgatcat atctacaggt
     2641 atgttctcgt cggacagcgg cgtgaacagg cgggcggcga caccgggacg gtccggaacg
     2701 tgaatgaccg tgatcttcgc ctgatctcta tcgtaagtga cgcccgaaac gactcctttc
     2761 tccatatcct tatcctcctt cgtaaccaaa gtcccggctc cgtcgttgaa cgaggaccgc
     2821 acatagacgg ggacctcgta ttttttcgcc aattccaccg aacgtgggtg caacaccttc
     2881 gccccggcgc ttgcgagctc gagcatctcc tcgtagcaga tttttttcag cagtctcgcg
     2941 ttgtcgcaga tgttcgggtc cgccgtgtag acgccgtcta cgtccgtata tatctcgcaa
     3001 acgtccgccc cgagggccgc cgccagcgcg acggcgctcg tgtccgaccc cccccgcccc
     3061 agcgtagtca cgttgttctt ccggtcgata ccctggaagc cggcgacgac ggcaacgtat
     3121 ccctgtctga ggtcctcttg aatcagggac cggtcgatct taagtatccg cgcccgcgtg
     3181 aaatccccgt ccgtgatgat cttcacctgg taggccaggt aggatttggc cttgcatccc
     3241 atcctgttca ggacgataga aaggagagca acgctgacct gttcacctga ggctatgagg
     3301 acgtcgtatt cgcgttcgat tgggtcgggg gccgcgctgt gggccagccg gatcaacctg
     3361 tctgtctcac ccgccatggc cgacaccacg acaacgaggt cgttcttcgc ctccctcgcc
     3421 ttgatgacct tcctggcgac gttttctatc ctttctatgt cggcgacaga ggtgccgccg
     3481 tatttttgaa ccacaagcgc catcagaaaa atcctccttc tttcagtgca tcctcgatcc
     3541 cgtcgaccct tcgccggaac ccctctatct ctatttcccg cctctcttca tcaagatagc
     3601 gcaagcggac gaagagggtt tcttccggca ggatgtcgag tatcttttcg gcccattcga
     3661 tgaccacgat accgtcgccg tagaaataat cctcgtatcc cagcggcaag aggtcctccg
     3721 acccctccaa cctgtatatg tctaagtggt aaagcctgta cctccccggg tactcgttga
     3781 tgagcgtgaa ggtgggactt gcgatgacgt aaccctcgtc gacggggatt ccctgcgcga
     3841 ttccctgcgt aagcaccgtc ttccccgacc ccagttcccc caccagcgca actacgtctc
     3901 ccggtccgag ccgcgccccg atgaccttcc ctatccggag cgtctcgtcg ggactctttg
     3961 ataccagact gaacgaacgc ctatcttctc ccatcgcccc ttacaccggc cgtccctcca
     4021 gaaccaccaa tgccgcggcg tagttgccgt cgtgcgacag gctcaggttc gccgcgccga
     4081 cccgtctttc ctcgacaacc tcggcggccc ttccgtagaa tctcagttct gggcgaccgc
     4141 cttcgcggac gaagacctcg acatccgtca gggctatccc ggcgaaaagg ccgtacccca
     4201 aggccttcag gaaggcctcc ttcgccgcga accgcgacgc gaagtgcctg ggcgggaaag
     4261 gacgtcgttt acagtactcg acctccccgg gcaggaagac cttcaccagg aatctgtcat
     4321 cccacctgat tatcgccttt tcgacccgcc ccacatcgac gaggtcgatc cccacgccga
     4381 cgatcatacc gatccccggg cgattgcgtc cgccatcgcc gcaacccgtt tcatgaaacc
     4441 gacgtcgtgc accctgacta tgtgggcgcc gttaaggatc gccgccgtca ccgtggccgc
     4501 tgttccctcc agcctctccg ccgcatcttt cccggtgacc gccccgatga acgccttgcg
     4561 ggacgtacct accaaaatgg gcctacccag caccttgaat tcgccgaggc ccctgaggag
     4621 gcggatgtta tcccgcaggc cctttccgaa cccgatgccc gggtccacta cgattctttc
     4681 ggggtctatc cccgcgtcaa gggcaagccg gatgctgccc gcgagataat cgatgacctc
     4741 ggagaaaagg tcgccgtaag aaaggtcgcc ttcctgcatc gtgcggggcg tgcccctcat
     4801 gtgcatcagg acgacggacg ccttgagctc cgccgcgacc tcggccatcc ggtcgtcgcc
     4861 gtttagggcc gtgatgtcgt tgacgagctc cgccccggcc tccagcgcgg ccttcgcgac
     4921 tttcgccttc gccgtatcga ccgagatggg aataccgacc ctggccgcga gcccctcgac
     4981 gaccgggatt acccgcctga gctcctcttc ctcggacacc gtcatggcgc cgggacgcga
     5041 cgactctccc cccacatcga ggatgtcggc cccttcttcc tccatctcga caccccgcct
     5101 taccgccgcc ccggcgtcga ccaattttcc tccgtcagag aaagaatcgg gcgtcacgtt
     5161 gataatcccc atgaccaggg tgcgcttgcc cagctcaata gtcctccgcg gcgtctccag
     5221 gcggaaagcg tccttcccta tgttttcgag aaccctcgcg atctccttcg ctatggcgct
     5281 caacccgaag ggctgtttct ctatcttctc cgcgaaccgc ccgacctgct tcctcgtccc
     5341 catcacgagg gcgtcggtct tttcgatgct gcaggctaca gtcccccttg ccacggccac
     5401 gtcgccgccg agggacagca tctcctgttt cattatgttc gccgtcgggc aaggcaggtc
     5461 tttgatgagg aggttcagat ggcccatctt cggcgccatc gcccggacac cgtaggcatc
     5521 cgccccgatc gactcgagga cttctgcggc ctccgcttcc gtgttgagca ccaagcacct
     5581 tatcggtccc acgatccgcc cggcatacac ctaatgaacc tggccgtctg cgaccttgcc
     5641 gtcgccgatg atcctgtcga tctcctccgc cgtcagcact tccttttcca gcagggcggc
     5701 ggcgagcctg tggagtttat ccaggttgtc ggcgaggagc ttcttcgcct tctcgtaact
     5761 ctcggacacg agccgcgata tctcccggtc gatcttttcc gccgtctcct cgctgtagtc
     5821 tttatgggtg gcgaactctc ggccgaggaa tatctgctcc tccttcttgc cgtaactcag
     5881 cgggcccatg ttctcgctca tcccccactc gcagaccatc tttctcgcca ggttcgtcgc
     5941 cctctcgatg tcgttacccg aacctgtcgt aaagtcgttg aggaccagct cttccgccgc
     6001 ccgtccgccg aggaggatcg tgagggcgtt catcaggtac tgcttcggat aggtatggcg
     6061 ctcgtccacc ggcaactgct gtgttatccc gagcgcccgg ccgcggggga tgatcgtcac
     6121 cttgtggatc gggtccgtcc cgggcaggag ccgcgccacg aaggcatgcc ccgattcgtg
     6181 gtaggccgtc gtcctcttct cttcctcgct tatgatcatg ctcttccgct cggtgcccat
     6241 gagcaccttg tccttcgcaa actcgaagtc gcacatccga acgcgctcct ggttcgcccg
     6301 cgccgccgcc agggccgcct cgttcacgag gttctcgata tcggcccccg aaaaccccgg
     6361 agtcccccgg gcaaggacgg cgaagtccac gtcgtcggcg agggggatct tccgcgtgtg
     6421 gacctcgaag atcttctc
//
LOCUS       DAOTMX010000058         6430 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_10376_length_6430_cov_4.629490, whole genome shotgun sequence.
ACCESSION   DAOTMX010000058 DAOTMX010000000
VERSION     DAOTMX010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 6430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6430
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10376_length_6430_cov_4.629490"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="P5573_02680"
     CDS             <1..384
                     /locus_tag="P5573_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27005.1"
                     /translation="GYDSDKKDIFEATLSFLLRNKVPAAYFNMLTPHKGSPLYDRLEA
                     EGRIVDVDGIGRWPGMTCHIKLKNFSATELIQQIKGLHRRFYSYRSMLVRLPLPLTEA
                     AVASWTINMAERKSFKEEAENFDEF"
     gene            374..1126
                     /locus_tag="P5573_02685"
     CDS             374..1126
                     /locus_tag="P5573_02685"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HRT27006.1"
                     /translation="MSSERTDERHALIIAAGRGSRLRMSTANRPKPMVEVGGTPLIRR
                     VIEAAGAAGIARFTVVTGYLGDVLEDYLARETPAGVKVSCIRNPEWERPNGVSVWKAR
                     GFVPREFALLMSDHLFDPRILRLLYSTPLEDGFCRLAADFNPGGVPDLEDATKVSAVG
                     GRLMDIGKEIRDYNAVDTGVFLCTEGIFSALEAAISRGEESLSAAVLELARRGKMEVT
                     DIGGLFWRDVDDEAGLLEAERAIVGKAVTFSY"
     gene            1191..2237
                     /gene="hpnH"
                     /locus_tag="P5573_02690"
     CDS             1191..2237
                     /gene="hpnH"
                     /locus_tag="P5573_02690"
                     /inference="COORDINATES: protein motif:HMM:TIGR03470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HRT27007.1"
                     /translation="MGIPFIQKYRIAHYIYRQRKNGRRHFPLVLMLEPLFRCNLSCAG
                     CGKVGYPEEILNKRLSVGECLSAAEESGAPVVSIAGGESLIHDEMPQIVAGLTGMKRF
                     VYLCTNGILLKKHITSYNPSPYLTFSVHLDGRRELHDRITGREGVFDRAVEAMDTLKA
                     GGFRFTVNCTIYSGASAEETAEFFDFVLHRGVEGITVSPGYRYEEAPDKDLFLERAES
                     KRLFREVLRIGRGRGWRFNHSTLFVDFLAGNRGYTCSPWGTVTRNIFGWQRPCYLFAD
                     GYVESIRSLLEETDWKRYGPGRNPRCECCMMHSGFEFSAISDMIAHPLRAFSVYLRGP
                     ATDGPLAGDPAESR"
     gene            2319..3371
                     /locus_tag="P5573_02695"
     CDS             2319..3371
                     /locus_tag="P5573_02695"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HRT27008.1"
                     /translation="MEGIRAIGTCDPDFRYESEILKKVSRSFALTIPELPDGLERAVT
                     NAYLICRIVDTIEDDEELPLDKKADFLEQFISVVNARESAEQFVAGVSPLLSDSIPEA
                     ERELVRHTPVVISGTFSLNPRQQKAIRDCTAVLARGMLDFQRKKTAWGLKDMAEFDQY
                     CYFVAGVVGEMLTELFCDYSPKIAERREKLLSLAASFGQGLQMTNIIKDLWEDRSRGV
                     CWMPRSVFAKAGYDLKDLTRICDSDSFGKGINEMIGVARHHLKNALTYTTLIPPEESG
                     IRKFCLWAIGMAVFTLRNVSKKLDYRSGKDVKISRRTLKSIIFVSDVAGHSNFLLERI
                     FELATRTLPGEKPLSH"
     gene            3465..4109
                     /locus_tag="P5573_02700"
     CDS             3465..4109
                     /locus_tag="P5573_02700"
                     /inference="COORDINATES: protein motif:HMM:NF017319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRT27009.1"
                     /translation="MKTKIIAIIMLCCFFLLAGTGSSLAQETTPTEAVRTMLERVMTV
                     QTDPLLQGQAHRNERRIAIKKIIEQNFYFDGMSRLALGPYWDKLGKTERAEFERIFKD
                     LFQDSYTKLVLDFLKREKIVYGQKEDGAQGNAVVKTRILRMNEEIPVDYSLVLVEGKW
                     LVQDVAIDGVSIIENYRRSFTRVIQRESFKELLRKMQIQQKAIEKSSGKEKAGR"
     gene            4123..5127
                     /locus_tag="P5573_02705"
     CDS             4123..5127
                     /locus_tag="P5573_02705"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HRT27010.1"
                     /translation="MRKFHITAIILGVTLLLFLVWKIGPGALWREFTVMGWGLLPFVL
                     IEGIVKVFHTVALRHCLSPPYSALPLGRLYKIYLAGSSINFFTPTATIGGEVVKGALL
                     TAEHPGSGAATGVIVGKLAYAFSQFFMVLLGLVVVLWKIPLPASAYAGLIVGSVLLGS
                     GIIGFLVVQRKGKLGAIIRWVADRSRSETLNRWAQYITKVDDDLRLFYRENPTGLPVA
                     ISWHMVGYLVSMLKTWYFLSLFTHEPFMTAAGIWLLSTWLDLMVFFIPLEIGLQESIR
                     VVVFSFLGFTMALGLTYGIALRVEQLFWGVAGLLIYMAILPSRQVVDVLPEVVVGDDR
                     "
     gene            5166..>6430
                     /locus_tag="P5573_02710"
     CDS             5166..>6430
                     /locus_tag="P5573_02710"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRT27011.1"
                     /translation="MARSYRFILSASALLGLVCMMAGAAVGQQPPKPAVLNLQQVVEK
                     AVSYSPEVAEAQNDVVSARSDLAQAESAYYPQIETMAIAGPVRDAKEPLVVDGRITDP
                     SPGLSFSSIGIFGKLDIIATQPLYTFGKISNRQEAASHGVRAKEHAIADRKGQIALRA
                     KQLYYGLVLARAGLDYADESKDFFDEAAQRIRNLLDVGSTNVKESDLYQVDSYRAEAT
                     RFRAEAEKGMRTAYFALKSMMGMPAGVDFEPADRNLPLRQEELRELDHYVREARTNRP
                     EFKQLSEALAAQEYMVKGAVSDRYPSFFAAAVGSFAGAPGRDHLSNPYISDDFNHAYG
                     GIVAGAKWSLDFGIAKAKIDKAKAEYNKLLNTKASAEMNIPIQVAKIYQDNVEALTAV
                     KTYKDAAVATRKWVVTAMADFDMGVGTAD"
BASE COUNT         1442 a         1699 c         1932 g         1357 t
ORIGIN      
        1 ggctacgatt ccgacaagaa ggatatattt gaggcgaccc tctcgtttct gctccgcaac
       61 aaggtgccgg cggcttactt caacatgttg acgccccata aggggtcacc cctctatgac
      121 cgattggagg ctgagggtag gatcgtcgat gtggacggaa taggccggtg gccgggcatg
      181 acgtgccaca tcaagctgaa gaatttttcg gctaccgaac tgatacagca gataaagggt
      241 ttgcaccgaa ggttctacag ctaccggtcg atgttggtca ggctgcccct gcctttgacc
      301 gaggcggccg tcgcgtcctg gacgatcaac atggccgaga ggaagagttt taaggaagag
      361 gctgagaatt tcgatgagtt ctgagcgcac ggatgagagg catgccctca tcatagccgc
      421 aggacgtggg agtcggttga ggatgagtac ggcaaaccgc cccaagccga tggtcgaagt
      481 cggcggcact ccgctgatca ggcgtgtgat cgaagccgcc ggtgccgccg ggatagcccg
      541 gtttacggta gtgaccggtt acctgggaga cgtgctcgag gattatcttg cccgggagac
      601 accggcgggt gtgaaagtaa gctgtatccg caaccccgag tgggagcgtc cgaacggtgt
      661 atccgtatgg aaggccaggg gtttcgtgcc gcgggaattc gctttgctga tgtccgacca
      721 tctttttgac ccccgcatcc tccggcttct ctactcaacg ccgctggaag acggcttttg
      781 ccgcctggcg gcggacttca acccgggagg tgtcccggac ctggaagatg cgacgaaggt
      841 ctctgccgta ggtggacggt tgatggacat cgggaaagaa ataagggatt acaacgcggt
      901 tgacacgggg gtgtttctct gcacggaggg gatcttttcc gccttggagg ccgccatctc
      961 ccggggcgaa gagagccttt cggctgccgt cctcgaactc gcccgcaggg gaaaaatgga
     1021 ggtaacggac atcggcggtc ttttctggcg ggacgtcgac gacgaggccg gcctgctgga
     1081 agcggaacgt gcgatcgtcg gcaaggccgt cacttttagc tattgagcaa aggtcctcct
     1141 tatacgaggt cgcaccttgg tgaaacacaa aaccctcggg attttgagat atgggcattc
     1201 cgtttattca gaaataccgc atcgcgcatt atatataccg tcagaggaaa aacggcaggc
     1261 ggcattttcc cctggtgctc atgctggagc cgcttttccg ctgcaacctc tcctgcgccg
     1321 gctgcggcaa ggtcggttac ccggaagaaa tcctgaataa acggctcagt gtgggggagt
     1381 gcctgtccgc cgcggaggaa tctggcgctc cggtcgtgtc gatcgcggga ggggaatcgc
     1441 ttattcatga tgaaatgccg cagatcgttg ccggcctgac cgggatgaag aggtttgttt
     1501 acctctgtac aaacggcatc ctcctgaaaa aacacataac atcttacaac ccttcgcctt
     1561 acttgacctt ttccgtccac ctggacggcc gccgggaact gcacgacagg atcaccggcc
     1621 gggagggggt cttcgaccgt gcagtcgaag cgatggatac actgaaggcc gggggtttcc
     1681 ggttcaccgt aaactgcacc atctacagcg gagcgagcgc agaggaaacg gcggaatttt
     1741 tcgattttgt tttgcaccgg ggggttgagg gaataaccgt atcgcccggc taccgttatg
     1801 aagaagcccc ggataaagac ctcttcctgg aacgcgccga gagcaaaaga ttattccggg
     1861 aggtattgag gatcggcagg gggcgggggt ggagattcaa tcattcgacc ctcttcgtcg
     1921 acttcctggc cggcaaccgt gggtatactt gcagcccctg gggcaccgtg actcgtaaca
     1981 ttttcggctg gcaaagaccc tgttatctct ttgcggacgg atatgtcgaa agtattcgga
     2041 gcttgctgga ggaaaccgat tggaagcggt acggcccggg gcgtaaccca cgttgcgaat
     2101 gctgtatgat gcacagcggg tttgaatttt cagcgatcag cgatatgatt gcccaccccc
     2161 tacgggcctt ttccgtctac ttgcggggac cggcaacgga cggtcccctc gcgggagacc
     2221 cggcggaaag ccgatgaaat ttttacggcc caaggtcttc cttaaatcgt caaaatgatg
     2281 taaaagggca aatcaagtcc aaatccggga ttgggtcgat ggaaggtatt agggccatag
     2341 gaacatgtga ccccgatttc agatacgaga gcgaaatact caagaaggtg tcacgctctt
     2401 ttgcgttaac gatccccgag cttccggacg gattggagag ggcggtgacg aacgcctacc
     2461 tgatttgccg gatcgtcgac acgatcgaag acgatgaaga actgccgctg gacaagaagg
     2521 ctgactttct cgaacaattc atcagtgtag tcaacgcgag ggaatcggcc gagcagtttg
     2581 tcgcaggcgt gtcgccgctc ttgtcggaca gtattcccga ggcggaaagg gaactcgtcc
     2641 gacacacccc ggtcgtgatc agcgggacgt tcagtctcaa cccgcggcaa cagaaggcaa
     2701 tcagggactg cactgcggtc ctggcccggg ggatgctcga tttccagaga aagaaaacgg
     2761 cctggggtct gaaagacatg gccgaattcg atcaatactg ctacttcgtg gccggtgtgg
     2821 tcggggagat gctgacggag ctcttttgtg attactcgcc taagatagca gaacggaggg
     2881 agaaactttt atcccttgcg gcgtcgttcg gtcaaggtct ccagatgacc aatatcatca
     2941 aagacttatg ggaagatagg agccgtgggg tatgttggat gccccgttcg gttttcgcaa
     3001 aggcagggta tgacctgaaa gaccttaccc ggatttgcga cagcgactcc ttcggaaagg
     3061 gtatcaacga gatgatcggg gtcgcccgac atcaccttaa aaacgcattg acttacacaa
     3121 ccctgattcc acctgaggaa tcggggatca gaaaattctg cctctgggcg atcggcatgg
     3181 ccgtcttcac tctcagaaac gtaagcaaaa agctggatta cagaagcggc aaggatgtca
     3241 agatatcccg ccggacgttg aagtccatca tatttgtttc tgatgtggcc gggcacagca
     3301 atttcctgct cgaaagaata ttcgaattgg ctacgcgcac tctgcccgga gaaaagcccc
     3361 tcagccattg acaggatctc acgtttctcc ccggattgac cttcatcagg ggaactatcg
     3421 gggcggcgga caagcccaat ctgaaaaaac tgaaggaagt atcgatgaaa accaagataa
     3481 tcgcaatcat catgttgtgt tgcttttttt tgctggcagg cacgggcagc tcgctcgcgc
     3541 aggagacaac gcctacggag gcggtcagga cgatgctcga gagagtcatg acggttcaga
     3601 ccgaccctct tcttcaggga caggcacacc gcaacgagcg tcgcattgcg ataaagaaga
     3661 taatagagca gaatttttat tttgacggca tgtcccgcct ggccctcggt ccgtactggg
     3721 acaagctcgg caagaccgag cgggcggaat tcgaaagaat cttcaaggat cttttccaag
     3781 attcttacac caagctggtg ctcgattttc tcaagcgcga aaaaatcgtc tacggccaga
     3841 aggaggacgg ggcgcagggt aacgccgttg tcaaaacaag gatcctccgg atgaacgagg
     3901 agataccggt cgattattct ctggtactgg tcgaggggaa atggctcgtc caagatgtgg
     3961 cgatagacgg ggtgagcata atagagaact accggcgctc ttttacccgc gtcatccagc
     4021 gtgaatcatt caaggagctc ctccggaaga tgcagattca gcagaaggcg atcgaaaagt
     4081 cttccgggaa agaaaaggcc ggacgataac tcatcagaac ccatgcgtaa attccatata
     4141 acggcaatca ttctcggggt taccctgctt cttttcctgg tctggaaaat aggccccggt
     4201 gccctgtggc gcgagttcac tgtgatggga tgggggctgc ttcccttcgt cctgatcgaa
     4261 ggcatcgtaa aggtattcca caccgtcgcc ctgcgacatt gcctttcgcc gccctactca
     4321 gccttaccgc tcggccgcct gtacaagata tacctggccg gcagctcgat caacttcttt
     4381 acacctaccg cgacgatagg gggggaggtg gtcaaaggcg cccttctcac ggccgaacat
     4441 cccgggtcgg gcgcggcgac aggcgttatc gtcggaaaac tcgcctatgc attctcccaa
     4501 tttttcatgg ttctcctggg ccttgtcgtc gtcctctgga aaattccgtt gccggcctcc
     4561 gcttatgcgg ggctgatcgt gggtagcgtc ttgctgggct cggggatcat cggcttcctg
     4621 gtcgtgcagc gcaagggaaa gttgggcgct atcatccggt gggttgccga ccgcagccgt
     4681 agcgagacct tgaatcgatg ggcacagtac atcacgaagg tcgacgacga cctcaggctc
     4741 ttttaccggg agaaccctac ggggcttccg gttgccatat cctggcacat ggtcggatac
     4801 ctggtcagca tgctgaaaac ctggtatttt ctttcgctgt tcacacatga gccgttcatg
     4861 acggccgcgg gcatttggct cctttcgact tggctcgacc tcatggtgtt ctttattccc
     4921 ctcgagatcg ggcttcagga aagtataagg gtggtggttt tcagtttttt ggggttcacc
     4981 atggcgctgg gactcactta cgggatcgca ttgagggtcg agcagctgtt ctggggcgtc
     5041 gcgggtctcc tgatttacat ggccatcttg ccgtcccggc aggtagtaga tgtgcttccc
     5101 gaggtagtcg tcggggacga ccgttagaag aagaagtatg gtgcaaagta tcgggggaaa
     5161 ttgaaatggc aaggtcatac cgtttcatcc tgtcggcgtc cgctcttctg gggttggtct
     5221 gtatgatggc gggcgcggcc gtaggccaac aaccgccgaa gccggcggtc ttgaaccttc
     5281 aacaggtggt ggagaaggcg gtctcctaca gcccggaggt ggcagaggcc cagaacgatg
     5341 tggtttccgc caggagcgac ctggcgcagg ccgagtcggc ctactatcct cagatcgaga
     5401 ctatggcaat agccggcccg gtgcgtgacg ccaaggaacc gctggtcgtg gacggcagga
     5461 taacggaccc ttctcccggc ctgagctttt cgagcatcgg tattttcgga aaactggaca
     5521 taatcgccac tcagccgctc tacacgttcg gcaagatatc caacaggcag gaggccgcct
     5581 cccatggggt cagggcgaag gaacatgcaa tagccgacag gaaaggccag atcgccctcc
     5641 gggcgaaaca actgtactac ggcctggtgt tggcccgcgc cgggttggac tacgccgacg
     5701 agtcgaagga ttttttcgac gaagccgccc aacgcatccg caatctcctg gacgtggggt
     5761 cgacgaatgt aaaggaaagt gacctctacc aagtcgattc ctatcgcgcc gaggcaaccc
     5821 gcttccgagc cgaggcggag aaggggatga ggaccgccta tttcgccctt aagtcgatga
     5881 tgggtatgcc cgcgggtgta gacttcgagc cggcggacag gaacctgccg ctcaggcagg
     5941 aagaattgcg cgaactcgac cattacgtgc gggaggcgag gaccaaccga ccggaattca
     6001 agcagctctc cgaggccctc gcagcccagg aatacatggt caaaggtgcc gtcagcgacc
     6061 gctacccctc cttctttgcg gcagccgtcg ggtcatttgc gggtgccccc gggcgggacc
     6121 atctgagcaa tccttacatc tccgacgatt tcaaccatgc atacggcggt atcgtggcgg
     6181 gggccaagtg gagcctggac tttgggatag ccaaggcgaa gatcgataag gccaaggccg
     6241 agtacaacaa actgctcaac acaaaggcct ccgcggaaat gaacatcccc atacaggtgg
     6301 ccaagattta ccaggataat gtagaggcat tgacggccgt gaaaacatac aaagatgccg
     6361 ccgtagctac tcgaaaatgg gtcgtcacgg ccatggccga tttcgacatg ggggttggga
     6421 ccgccgacga
//
LOCUS       DAOTMX010000059         6427 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_10381_length_6427_cov_4.530917, whole genome shotgun sequence.
ACCESSION   DAOTMX010000059 DAOTMX010000000
VERSION     DAOTMX010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 6427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6427
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10381_length_6427_cov_4.530917"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(351..1292)
                     /locus_tag="P5573_02715"
     CDS             complement(351..1292)
                     /locus_tag="P5573_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27012.1"
                     /translation="MSSDEILRKSVLLGLRNGWNGFVWMMKIIIPISFCTELLAWSGW
                     LQKADVFFRPFMKLLGLPAMAAYPLLVGATTGIYGGIAVMVALPFTKEEMTLMALFLL
                     VAHNLVQEGIVQGKSGINPWKATFIRLFAAALTVAVLAPLLVGGGSHVAETPVVLSSH
                     VKPLGETVLSWALTMLRLAIRILLIILTIMTFLEVMKVKGWINGLVRFLSPVLQVMGL
                     SREVGFVWLTGAIFGLAYGGSVIIQESRAGHLSREDIETLHLSLGINHAMVEDPALFL
                     SLGLSPFWLWVPRIIVAILMIRLFTLWRKVFPRKIAA"
     gene            1547..2059
                     /locus_tag="P5573_02720"
     CDS             1547..2059
                     /locus_tag="P5573_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27013.1"
                     /translation="MAEIKSTLDLVMERTKNFTLTDGEKRELRRRELEGRLRGLAQKS
                     LDGRLTVEETKGEFNSIVAGREEEARELMKEILADHIDPEGDHGRILEILRVVLGIET
                     GPLEGALRGLQEEIAAAREEYMERMKEDLARRQVFGTAVVPNLSRSPEWQEFRKKAIS
                     TLKGRLLSRP"
     gene            complement(2087..4216)
                     /locus_tag="P5573_02725"
     CDS             complement(2087..4216)
                     /locus_tag="P5573_02725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRT27014.1"
                     /translation="MHSLLRRQIQRHFGGLEGIPDKLDVFIKLIDDTYHEFDKDRELL
                     ERSLELTSQEMLQVNAEMRALLRAMPDLLITLDSDGTVLHPPVGRLDDLVIPREDIVG
                     KKIYAIPNKEVRKRFRDALEKIKETKAGVTFEYSLLVNGSMNVYEARLFPINSHRNFV
                     IIRNITEWMEAEASLLESKRQLQKRNRELRRTKNFLQGILDSSADCIVTADLAGTLMY
                     VTPSVKKMTGYEPAEVTGKKVYDFYEKGIEDARTIIEKVREKGELRDHSLRVRGKSGE
                     LIDVSVSVSPMVDENGAPLGTMGIVRDVRERKKLEAQLMQAQKMEAIGTLAGGIAHDF
                     NNLLMGIQGYASLVLLDLDEGHPHFYKLKCIQELVKSGAALTGQLLGFARGGRYEVVP
                     ADLNEIAKLSVSLFGRTRKQVRFHERYGRDLWAVEVDVGQMRQVFLNLFVNAFDAMPE
                     GGDVYVETENVLLDEKYAEYCGVRPGPFVKLSVTDTGPGMDESTIGRIFEPFFTTKEM
                     GRGTGLGLATVYGIVKGHGGIIDVHSRVGYGTTFNIYIPMSEKERKREVRPHQKVAHG
                     SETILLVDDEEVILEVSREMLESLGYKIFWADNGRDAVEIYAANKGKIDLVILDMIMP
                     GMSGSETFDKLKEINPHVKVILSSGYSMNGMAANIMEKGCRGFIQKPFNFEQLSSSVR
                     QALAADRQTRRLVRLNMPGKMTSRQGG"
     gene            complement(4229..5374)
                     /locus_tag="P5573_02730"
     CDS             complement(4229..5374)
                     /locus_tag="P5573_02730"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HRT27015.1"
                     /translation="MKIEQREWTHGEGWVPDGPSPLKESAQLVLVFGATPLLKDDGCL
                     YRIRKAYPRADFIGCSTAGEICGPRVFDDSLVTTAVHFESTTVNVVSMEIMDREESFE
                     VGKKLAQSVGHEGLVHLFVVSDGLNVNGSALVQGLSACLPEGVTISGGLAGDGERFAE
                     TLVVGNGPPARNTVAVAAFYGEGLRVCCCSFGGWDPFGPERLVTRSRGNVLYELDGRS
                     ALEIYKQYLGTYARGLPATALLFPLSIRFAEDQKGVVRCILGIDEDEKSMTFAGDIPK
                     GAYARFMKANADRLVDGAVKTARSCIQGCRDYDQEPDLAVLISCVGRKMVLKQRVEEE
                     IEGVQEVLGSRTAFAGFYSYGEIAPSLPGGKSELHNQTMTVTTFLEK"
     gene            complement(5409..6044)
                     /locus_tag="P5573_02735"
     CDS             complement(5409..6044)
                     /locus_tag="P5573_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRT27016.1"
                     /translation="MEITTGLHAFIWQNPRANNANTYLIAGKKNILIDPGHLHLFDHV
                     INGLGRLGITPAQVDVVLVTHAHADHMEAVALFGESTLVAMGRESFQFIEGNGGKTRE
                     PEFFLREGNLTVGDVSLDVLMTPGHCPGSLSFYWPDRKALFTGDVIFSQGIGRTDLPG
                     GDGRSLKESIERLSVLDVEYLLPGHGGAVVGKEKVKDNFRMVAEYWFGYLG"
     gene            6199..>6427
                     /locus_tag="P5573_02740"
     CDS             6199..>6427
                     /locus_tag="P5573_02740"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HRT27017.1"
                     /translation="MAVKILIKRKVPREKGMALLNLIGELRVRAVAQPGYISGETMRS
                     IDRPDEYLVISTWQSIDDWKAWAASKERAEIQ"
BASE COUNT         1420 a         1896 c         1668 g         1443 t
ORIGIN      
        1 tcttcatgac cgccgagagg gttggggtct tttttccact catttaccca atcccaattc
       61 actttgccgt tactcatctc tcgtacctcc ttctacaatc tcaaaagaat atcgcttggt
      121 tatacttttg acaagactcg acccgctttt tccatcacct cccttccctt atcgggcacc
      181 atacacaact tttgaatatc acatccgttt ttacaagata ctataaacgc ttaataagcg
      241 ctcatatact caacttttta ccgataatac aatatatgat gtatttgtca agaaatatcc
      301 tcggccccgg ggacgtctcc gctaccctcg cgcctaagga aaaccctttt ctatgccgct
      361 atcttccggg gaaaaacttt acgccagagc gtgaagaggc gtatcatgag gatggccacg
      421 attatcctcg ggacccagag ccaaaagggg ctcaggccca aggaaaggaa aagcgcgggg
      481 tcttccacca tggcgtggtt gatcccgagt gaaaggtgga gcgtctcgat gtcctcccgg
      541 cttaggtgtc ccgcccgcga ttcctggatg atcacgctcc cgccgtaggc caggccgaag
      601 atagcgccgg tcagccagac gaaccctacc tcccggctca gccccatgac ctgcaagaca
      661 ggagagagga atcgcacgag gccgttgatc cagcccttga ccttcatgac ctccaggaat
      721 gtcatgatcg tgagtatgat caggagaatc ctgatagcca gtctcagcat ggtcaaggcc
      781 caggacagga ccgtttcacc gaggggtttc acatgggaag agaggacaac cggggtctcc
      841 gccacgtgag aacccccgcc gacgagcagg ggagcgagaa ccgcgactgt cagcgcggcc
      901 gcaaagagtc ggatgaaggt ggccttccag ggattgatac cggacttgcc ctggacgatc
      961 ccttcctgaa ccaggttgtg ggcaacgagg aggaaaaggg ccatgagggt catttcctct
     1021 ttcgtgaagg ggagggccac catcacagcg atccccccgt agatgccggt ggtcgcgccg
     1081 acgagcagcg ggtaggcggc catggccggc aggcccagca atttcatgaa gggccgaaag
     1141 aaaacatccg ccttctgaag ccacccgctc caggcaagaa gctccgtgca gaacgagatc
     1201 ggaatgatga ttttcatcat ccagacaaag ccgttccacc cgttgcgaag cccaaggagc
     1261 acgctcttac gaagaatctc gtcggaactc acgccgtctc tctaactgtg atgtcgtcct
     1321 ggggctcaca tgcagtcgct cacgcccccc tgttgctcgg ccccatccta attcggcatc
     1381 caagaaaact ccataccaat atactgcgaa accgggcttt tctattcgct ctcgacgcaa
     1441 ttattagcat ccaccggata actgttgaaa atatcgcgtg attacgctat gttttgccgt
     1501 gacctcgtct tgcgagagaa aacgggtcgg agaaaggaaa acccttatgg cggagataaa
     1561 aagtacgctg gacctcgtga tggaaaggac gaagaacttt accctgacgg acggggaaaa
     1621 acgggagctc cggcgcaggg aattggaagg aaggctcaga ggactggcgc aaaagtccct
     1681 cgacggacgg cttactgtcg aggaaaccaa aggagaattc aacagcatcg tagcaggccg
     1741 ggaggaggag gcacgggaac tcatgaagga gattctagcc gaccacattg acccggaagg
     1801 agatcatggc cgtatcctgg agatcctccg ggtcgtcctg gggatagaga cggggcctct
     1861 ggagggggca ttacggggcc tccaggaaga gattgccgcg gcaagggagg aatacatgga
     1921 aaggatgaaa gaagacctgg cgcgccgcca ggtctttgga accgccgtcg ttcccaacct
     1981 ttcccgcagt cccgagtggc aggagttccg caaaaaagcg atctctaccc tcaaaggccg
     2041 ccttctttct cgaccatagc aaccgcctcg cgttgcccgc tcctcttcaa cccccttgac
     2101 gcgatgtcat tttcccgggc atattcagac ggaccagccg tcgtgtctgc ctgtcggcag
     2161 ccagcgcttg ccgtaccgac gaagaaagct gctcgaagtt gaaaggtttc tgaataaaac
     2221 cccggcaccc cttttccatg atgttcgcgg ccatcccatt catgctgtat ccgctcgaca
     2281 gaataacctt cacatggggg tttatctcct tgagcttgtc gaaggtctcg ctgccgctca
     2341 tgccgggcat gatcatgtcc agtatcacaa ggtcgatctt ccctttgttc gccgcataga
     2401 tctcgacggc gtcccggccg ttatcagccc agaaaatttt atagccgagc gactccagca
     2461 tctccctgct cacctcgagg atcacctctt catcatcaac gagaaggatc gtctcgcttc
     2521 cgtgcgctac cttttggtgc ggtcggactt ctcgctttcg ttctttctcc gacattggga
     2581 tgtaaatatt gaatgtcgtg ccgtatccta ctcgactgtg aacgtctatg ataccaccgt
     2641 gccccttcac gattccgtag acggtcgcaa ggccgagtcc ggttcccctt cccatttcct
     2701 tggtcgtgaa aaagggctca aatatcctgc cgatagtgct ttcgtccatc cccgggccgg
     2761 tatcggtaac cgacaacttg acgaacgggc ctggccttac cccgcagtat tcggcatact
     2821 tttcatcgag aaggacgttt tccgtctcga cgtaaacatc ccctccttcc ggcatggcgt
     2881 cgaaggcatt aacaaaaaga ttgaggaaga cctgccgcat ctgaccgaca tcgacctcga
     2941 cagcccagag gtccctgcca tacctctcgt ggaatctcac ctgttttcgc gtccggccga
     3001 acaaggaaac ggagagtttg gctatctcgt tgaggtcggc gggcaccacc tcgtacctcc
     3061 cacccctggc aaagccgagg agctgccccg tcaacgcggc cccgctcttt accaactctt
     3121 ggatgcattt cagcttgtag aaatgggggt gcccttcgtc taaatcgagg aggacaaggg
     3181 atgcataccc ctgaatgccc atgagcaggt tgttgaaatc atgagcgata ccgccggcca
     3241 gcgtgccgat cgcctccatc ttctgggctt gcatgagctg tgcctccagc ttctttcttt
     3301 ccctcacatc ccttactatt cccatggtac ccagcggggc cccgttttcg tcgaccatcg
     3361 gggatacaga cacgctgacg tcgataagct ctccactctt tcccctcact ctgagcgaat
     3421 ggtccctcag ttcgcccttc tctctgacct tctcaatgat ggtgcgggcg tcctcgatcc
     3481 ccttttcgta aaagtcatag accttcttgc ctgtcacttc cgcaggttca tagcctgtca
     3541 ttttcttcac gctcggtgta acgtacataa gggtgccggc aaggtcggcc gtaacgatac
     3601 aatcggcgga gctgtcgagg ataccctgga ggaaattctt cgttctcctc agttccctgt
     3661 ttcttttttg aagttgccgt ttgctctcca gaagggaggc ctccgcctcc atccattcgg
     3721 tgatgttccg gatgatgacg aagttccggt gcgagttgat ggggaagagc cgggcctcat
     3781 atacgttcat agacccattc acaaggaggg aatattcaaa ggttacaccg gctttcgtct
     3841 ccttgatttt ttcaagggcg tcccggaatc ttttccttac ttctttgttc ggtattgcgt
     3901 agattttttt acctacgata tcttcccggg gtataacgag gtcatcgaga cgaccgacgg
     3961 ggggatgcag aaccgtcccg tcggagtcga gcgttattag gaggtcgggc attgcacgca
     4021 gaagggcccg catctcggcg ttgacctgga gcatctcttg cgaggtcagt tcgagggacc
     4081 gctcgaggag ttctctgtcc ttgtcgaact catgataggt gtcgtcgatc aattttatga
     4141 agacatcgag tttgtcgggg atcccttcga gcccaccgaa atgtctttgt atctggcgtc
     4201 gcaataaact gtgcatcgct catctaacct atttctctaa aaaagtcgtg accgtcatgg
     4261 tctgattgtg caactcactc ttcccccccg gtaaagaagg tgcgatctcc ccgtaggagt
     4321 agaacccggc gaacgccgtc cgcgaaccca gcacctcctg cacgccctct atctcttctt
     4381 ccacccgctg tttcagcacc atcttcctcc ccacacaact tatgagtacg gccaggtccg
     4441 gctcctggtc atagtccctg cacccttgaa tgcagcttct tgccgtcttc acggcgccgt
     4501 cgacgagccg atcggcattc gccttcatga agcgggcata cgctcctttc gggatatccc
     4561 ccgcaaaggt catgctcttt tcgtcttcgt cgatgcctag gatacaccga acaacgcctt
     4621 tttgatcttc agcgaagcga atgcttaagg ggaaaagtag cgctgtcgcc ggcaagccgc
     4681 gggcataagt acccaggtat tgtttgtaga tttcaagggc ggaccttccg tccagttcat
     4741 agaggacatt tccccgcgac cgcgtcacca gcctctcggg gccgaagggg tcccatccgc
     4801 cgaaggaaca acaacaaaca cgcagtccct ctccgtaaaa cgccgcgacg gcgaccgtat
     4861 ttctggccgg aggcccattg ccgacgacaa gcgtctccgc gaaccgttca ccgtcaccgg
     4921 ccaaccctcc cgaaatcgtt accccttccg gcaaacatgc cgacaggcct tgtacgagtg
     4981 cgcttccgtt cacgttgagg ccgtccgaga cgacaaacag gtgaacgagg ccctcatgtc
     5041 cgacggactg ggccagcttt ttccccactt caaaactctc ttctctgtcc atgatctcca
     5101 tcgaaaccac gttgaccgtc gtggattcga agtgtaccgc cgtggtgacg agggagtcat
     5161 caaagacccg agggccacat atctccccgg cggtcgagca accgatgaag tcggccctgg
     5221 ggtaggcctt tctgatacgg tataagcagc cgtcatcctt caacagcggc gtcgcaccga
     5281 acacaagcac cagttgggcc gattctttca agggggaagg gccatcggga acccacccct
     5341 ctccatgggt ccattcacgc tgctcaattt tcatgtcagg gctcaccttt catgaatatg
     5401 ctcatctgtc accccaggta accgaaccag tactccgcaa ccatgcggaa gttgtctttg
     5461 accttctctt taccgaccac ggcccctccg tggccgggaa gaaggtattc gacgtccaag
     5521 actgaaagtc gctctatgct ttccttgagc gatcttccgt ccccgccggg gaggtctgtg
     5581 cgcccgatac cctggctgaa gatgacgtct cccgtgaaga gggccttccg gtcgggccag
     5641 taaaagctga gggaaccggg acaatggccc ggggtcatca ggacgtcgag agaaacgtct
     5701 ccgactgtga ggttgccttc ccgcaaaaag aattccggct ccctggtctt gccgccgtta
     5761 ccttcgataa actgaaagga ctcccggccc atggcaacga gagtcgactc gccgaaaagg
     5821 gcgacggcct ccatatggtc ggcgtgggcg tgcgttacca ggactacgtc gacctgcgcc
     5881 ggggttatgc cgagacggcc gaggccgttt atgacgtgat cgaagagatg aagatggcca
     5941 gggtcgatga ggatgttttt cttgcccgct atgaggtagg tgtttgcgtt gttcgccctg
     6001 gggttttgcc agataaaggc atggagaccc gtcgttattt ccatggtggt tgctctttct
     6061 gcttgaaaac tgcggcggaa gtttatatca taaatccaca aaaagtcacg caatcagttg
     6121 attgcatctc gagtctacac cttaacccac atcccctgtc ggggtccgca tgcaaattct
     6181 caatcaggag gtcgaacgat ggcggtcaaa atcctcatca aaagaaaagt cccccgggaa
     6241 aaaggcatgg cgctcctcaa tctgatcggt gaactccggg tacgggccgt agcccaaccg
     6301 ggatacatat ccggggaaac catgaggagc atcgaccggc ccgacgagta tctggtcatc
     6361 agcacctggc aaagcatcga cgactggaag gcctgggcgg caagcaagga acgggccgaa
     6421 atccagt
//
LOCUS       DAOTMX010000060         6349 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_10547_length_6349_cov_3.536702, whole genome shotgun sequence.
ACCESSION   DAOTMX010000060 DAOTMX010000000
VERSION     DAOTMX010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 6349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6349
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10547_length_6349_cov_3.536702"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1759)
                     /gene="secA"
                     /locus_tag="P5573_02745"
     CDS             complement(<1..1759)
                     /gene="secA"
                     /locus_tag="P5573_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HRT27018.1"
                     /translation="MIGYVLKKIIGSKNDRELKRLSVILDEINGLEESIKRLTDAQLQ
                     AKTGFFKERLEQGAELDDILPEAFAVAREAARRTLGMRPFDVQVIGGLVLHEGKIAEM
                     KTGEGKTLAATMPVYLNALKGRGVHVVTVNDYLAKRDAQWMGPVYRFLGLSVGVIVNG
                     IEDDERRAAYNADITYGTNNEFGFDYLRDNMKFSLDDYVQREFYYAIVDEVDSILIDE
                     ARTPLIISGSSEESTDKYYRINQIIPRLRKDRDYTVDEKTRTVVLTEEGVAHAEKLLK
                     VANLYDPRNIEILHHVNQGLKAHTLFKRDVDYLVKDGQVIIVDEFTGRVMPGRRYSDG
                     LHQALEAKEHVKIERENQTLATITFQNYFRMYEKLAGMTGTADTEAQEFKEIYGLDVV
                     VVPTNMPMIRVDHPDLIYKTEKEKFKAVVEEIKELNKLGRPVLVGTISIEKSELLSKM
                     LTREGIKHHVLNAKHHEREAEIIAQAGQRGAVTISTNMAGRGTDIKLGPGVAELGGLH
                     VLGTERHESRRIDNQLRGRSGRQGDKGSSRFYLSLEDDLLRIFGADRISSLMDRVGFE
                     EGQPIEHNLISKAVENAQKR"
     gene            complement(1853..2899)
                     /locus_tag="P5573_02750"
     CDS             complement(1853..2899)
                     /locus_tag="P5573_02750"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRT27019.1"
                     /translation="MTGRTRDKALHYPRVIFSHLQIISIKLRDLVVLLLSKVVECGFR
                     LKKRIQTSMDHYTVLIVPQKKSTVKKISATSTHVLWAVSSVAVLIMASCYFTYSYLSF
                     KMDYSELIALKQLSEAQKKQLDTLASKVGYFEKRMESLREYENRIRVMAELVKPKGQY
                     LGVGGPSREDTLGRHEAERETRSLSRIHQSMDQLIAEADQKERSFKEIIEFLEKKKSI
                     LSRTPSIWPVQGWVTSEFGSRPSPFSGAREFHAGVDIASRSGKEVVAPADGIVSEAER
                     RADLGNAVVVDHGNGLSTLYGHLLQCAVTKGKFVRRGEVLGYVGSTGKSTGSHLHYSV
                     YLNNIPVNPRKYLP"
     gene            complement(3074..3886)
                     /gene="mutM"
                     /locus_tag="P5573_02755"
     CDS             complement(3074..3886)
                     /gene="mutM"
                     /locus_tag="P5573_02755"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HRT27020.1"
                     /translation="MPELPEVETLCRQLDKVIPGSLVRSVTIQDAKLGEQRGLEEKKV
                     VRVRRSGKKIVVEFADCTRLLIHLRMTGRLLWAVNEEKIPHTRMTVSLDKGNLYLVDP
                     RRFATVSTDSCGGSAEGDGGTKIVGLGAEDLMTKARGKRRSVKTFLMDQRVVEGIGNI
                     YACEILHSSCINPSKKAGDLTIREWTKVVRSMNVILKKAIDCRGTTISDWRDLYGLPG
                     SFQHNLRVYGREGLPCFRCGSAVVRTVLNGRGTFYCPSCQGGPGICGDSSDA"
     gene            complement(3920..5863)
                     /locus_tag="P5573_02760"
     CDS             complement(3920..5863)
                     /locus_tag="P5573_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HRT27021.1"
                     /translation="MKIPQEIIDERITPSEFREKGVSHVFERLRKARGFYVTNTERDL
                     SQSDFKNRIMPHTQILVAEERNAAGFFSLEISGGASVHVDMLRKQISPLEKLEILSKS
                     MPDTMFQTLCRGVNLFGYRPYPENVIRLTVKSFARYIQVWRVFDFLNYVPNMIPVFEE
                     VKAAGCILEPSICFSTGPEHTNEFYVRKVGEIIEAAGEDIILCIKNHGGLGTQRRIGE
                     LVNAILQKFPDLIIHYHGHNTDGNDIGRTVEAVRNGAKIVDGGDHSITGFFGPPPLLT
                     ILDTLEDYGHHAMGIDRQAIIDTSNRLRAEREYYRDFESQFLGFDPTVQTHKLPGGAT
                     GSSFEQAVKGEFLHLMPRILQDELPKVQVELGNWWSVTPGSQILWTTAVNNVLKKDRY
                     KDVNDDLKNLMLERYGKLPFYKPADWIYEKVFGKDWKAFVEREGGYQKIEDIDIDIER
                     RVLEHRLGRKATEDELVLYLQHPNDAVGFFKFQEKFGKTWALPPKIWFHRGGFNLGDR
                     FEFADGYGKLHTIEIGPQRRTKSGDVITYIIIDHHTEPILTVFEEEAAREKAKPTLMA
                     KEIAALAKSGDVRSPIKGTVNEVPVSSGADVSAGEVLVVLEAMKMLTNVLAEVSGRIS
                     EVFVQPGDSVEVGDKLVAISLPE"
     gene            complement(5921..>6349)
                     /locus_tag="P5573_02765"
     CDS             complement(5921..>6349)
                     /locus_tag="P5573_02765"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417474.1"
                     /note="catalyzes the ATP-dependent carboxylation of a
                     covalently attached biotin and the transfer of the
                     carboxyl group to pyruvate forming oxaloacetate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HRT27022.1"
                     /translation="VRINAEDPKNNFMPEGGKRVEVYQSPGGPGIRLDGIVYQGYNIP
                     MDYDSLLVKLTVRGYQWEAVVQRLKRALNDFLIVGPKTTIPFYLAICDEPDFRRGVFD
                     TSYLDTHPEIFDYPEQEREIARLARLIAEIHARKHNPYAA"
BASE COUNT         1232 a         1885 c         1638 g         1594 t
ORIGIN      
        1 cgcgcttctg ggcgttttcg accgccttgg agatcaggtt gtgctcgatc ggttgtcctt
       61 cttcgaaccc aaccctgtcc atgagggaag aaatcctgtc ggcgccgaag atgcgcaaga
      121 ggtcgtcttc cagtgacagg taaaatctcg acgagccctt gtctccctgc cggcccgaac
      181 gccccctgag ctgattgtcg atccgtcggc tctcatgacg ctccgttccc aggacgtgaa
      241 gcccaccgag ttcggcaacg ccgggaccga gctttatgtc cgtgccgcgg cccgccatgt
      301 tagtggaaat cgtcacggcg cccctctgac ccgcctgggc gatgatctcc gcttcccgtt
      361 cgtgatgctt tgcgttcaag acgtggtgtt tgatcccctc ccgggtgagc atcttactga
      421 ggagctccga cttctcgatg gatatagtgc ccaccaggac aggtctcccc agtttgttca
      481 actcctttat ctcctcgacc acggccttga atttttcctt ttccgtcttg tagatgagat
      541 cgggatggtc gacgcggatc atcggcatgt tcgtgggtac gacgacaacg tcgagaccgt
      601 atatctcctt gaactcctgc gcctccgtgt ccgccgtccc ggtcataccg gccagcttct
      661 cgtacatacg gaagtagttc tgaaaggtga tcgtggcgag cgtttgattt tccctctcga
      721 tctttacgtg ctccttcgcc tccagcgcct gatgcagtcc gtcgctgtac cgcctgcccg
      781 gcatgacacg gcccgtgaac tcgtcgacga tgatgacttg accgtccttc acgaggtagt
      841 ccacgtccct cttgaaaagg gtgtgggcct tcaatccctg attgacatga tggagtatct
      901 cgatgttcct ggggtcgtag agattcgcta cctttagcag tttctccgca tgggccacac
      961 cttcctcggt caggacgacc gtccgagttt tctcatcgac ggtgtaatcc ctatccttcc
     1021 gaagacgggg aatgatctgg tttatccggt aatacttatc cgtcgattcc tctgaggatc
     1081 ctgatatgat gaggggggtc ctggcctcat cgatgaggat gctgtcgacc tcatccacga
     1141 ttgcatagta gaattccctc tggacataat cgtcgaggct gaatttcatg ttgtcgcgca
     1201 gatagtcgaa gccgaactcg ttattcgtgc cgtaggtgat gtcggcgttg tacgcggccc
     1261 ttctttcgtc gtcttcgatg ccgttgacga tgacgcccac ggaaaggccg aggaacctat
     1321 atacggggcc catccactgg gcgtcccgct tcgccagata atcgttgacc gtgacgacgt
     1381 gcacgcccct gcctttcaag gcattgaggt agaccggcat cgtcgccgcg agcgtcttac
     1441 cttcaccggt cttcatctcc gcgatcttac cctcatgaag gacgaggccg ccgatcacct
     1501 ggacgtcgaa tggtctcatt ccgagggtgc gtctcgccgc ctcccgggcg actgcgaagg
     1561 cctcgggcag tatgtcgtcc aactctgcac cctgctccag cctttccttg aagaacccgg
     1621 tcttggcctg aagctgagcg tccgtcaatc tcttgatcga ttcttccaag ccgtttattt
     1681 catcgagaat gacggagagc cgcttaagct cgcggtcgtt cttgctgccg attatctttt
     1741 tgaggacata tccgatcatt tcgtcaccac ataaaaaaac cggtaatatt ttaccggaat
     1801 ctcgccgaat tacaactgaa cgtccctacc gacgttttgc agtcagccga tgtcagggca
     1861 aatactttcg tgggtttaca ggaatgttgt tgagatagac gctgtaatga agatgggagc
     1921 ctgtactttt tcccgtactg ccgacgtaac ccaggacctc tccccgcctg acgaattttc
     1981 cctttgtgac ggcacactgg agaaggtgcc cgtataacgt gcttagcccg tttccatggt
     2041 caacaacgac ggcgttaccg agatcggccc ttcgctccgc ttcggatacg atcccgtcgg
     2101 cgggagcgac gacctccttg ccgctcctcg acgcaatgtc gaccccggcg tgaaattctc
     2161 tcgcgccgct gaagggcgac ggccggctcc cgaattccga tgtcacccat ccttggaccg
     2221 gccatatcga gggcgtacgg gagagtatcg attttttctt ctccaggaat tcgatgattt
     2281 ccttgaagct gcgttctttc tggtcggctt ccgcgatgag ctggtccatg ctctggtgga
     2341 ttcgactgag cgagcgtgtc tcccgctcgg cctcgtgcct tccgagtgtg tcttcccggg
     2401 atggaccgcc gacgccgagg tattgtcctt ttggcttcac gagttcggcc atgaccctta
     2461 tcctgttctc atactcgcga aggctttcca ttctcttctc gaaatatccg accttcgacg
     2521 ccagggtgtc aagctgcttt ttctgtgcct cggaaagttg cttcaaggcg atcagttccg
     2581 agtagtccat cttgaaactc aggtaactgt aagtgaagta gcaagaggcc atgatgagga
     2641 ctgcgacgga agaaaccgcc cataagacat gagtgcttgt cgccgatatc tttttcaccg
     2701 tgcttttttt ctgcggaacg atgagcacgg tgtaatggtc catcgacgtt tggatccgtt
     2761 ttttgaggcg gaacccgcac tccacgacct tcgacaacag aaggaccacc aaatcacgca
     2821 gtttgattga gatgatctga aggtgagaga agatgactct cggatagtgc aaggccttgt
     2881 ctcgcgttct tcctgtcact tcattccttc cagagggaat ttgtctaccg gaacaacctc
     2941 atacaaagcg cgctaaacct accagcaaac gaccgacttg tcaagaaatg attgaccgat
     3001 cgggctggca ataaaatatt acgcaatgat tacagcatga tgcctgaatg ggcccccctc
     3061 ccgtcgaggg agttcaggca tccgatgaat ccccgcatat tcccggcccg ccctggcagg
     3121 agggacaata gaaagttccc ctaccgttca acacggttct gactacagcc gaaccgcacc
     3181 ggaagcaggg cagaccctct cgcccgtaaa ccctcaggtt gtgctgaaat gacccgggca
     3241 ggccgtagag gtctctccag tcggaaatcg tggtaccgcg gcagtcgata gcttttttca
     3301 ggatcacgtt catcgaccgg acgaccttcg tccattccct gatggtcaaa tcacctgcct
     3361 tcttggacgg gtttatacag gagctgtgga gtatctcgca ggcatagatg tttccgattc
     3421 cctcgacgac ccgttggtcc atgaggaagg tctttaccga ccttctttta ccccgggcct
     3481 tcgtcataag gtcttccgcg ccgagaccga cgattttcgt accgccatcc ccttcagcac
     3541 tccccccaca cgaatccgta gacaccgtcg caaacctcct tgggtccaca agatagagat
     3601 tgcccttgtc gagagagacc gtcatgcggg tgtggggaat tttttcttcg tttacggccc
     3661 agaggagtct gcccgtcatc cgcaggtgaa tcagcagacg ggtacagtcc gcgaattcta
     3721 caacgatctt ctttcccgac cgccggacgc ggacgacctt cttctcctcc aggcctcgct
     3781 gttcgccgag tttggcgtcc tgaatagtca cggacctgac cagcgacccg ggaatcacct
     3841 tgtcgagttg gcggcagagg gtctctactt cgggaagctc aggcatcggt atcccggact
     3901 atccgtccca ccccatcctt tactcgggca agcttatggc caccagcttg tccccgactt
     3961 caacagaatc gcccggctgg acaaacactt ccgagattcg gccgctcacc tccgccaaga
     4021 cgttcgtcag catcttcatc gcctcgagca caaccaacac ttctcctgcc gagacatcgg
     4081 ccccgctgct taccggaacc tcgttgaccg ttccctttat aggagacctg acgtcgccgg
     4141 attttgcgag ggccgcgatc tccttggcca tcagtgtcgg tttcgccttc tcacgggcag
     4201 cttcttcttc gaacaccgtg aggatgggct ccgtatggtg gtcgatgatg atataggtta
     4261 tcacgtcgcc gctcttggtc ctgcgctgcg ggccgatctc tatcgtatgt agttttccgt
     4321 atccgtcggc aaactcaaag cggtcaccga gattgaagcc cccccgatgg aaccatatct
     4381 tcggcggcaa cgcccaggtc ttcccgaatt tctcctggaa cttgaaaaaa cccactgcat
     4441 cgttggggtg ctgcaagtaa aggacgagct cgtcttccgt cgcctttctt cccagtcggt
     4501 gctccaagac ccttctttcg atgtcgatgt cgatgtcttc gatcttttgg taccccccct
     4561 cccgctcgac gaaggccttc cagtccttgc cgaagacctt ctcgtatatc caatccgccg
     4621 gcttgtagaa gggaagcttc ccgtaacgtt ccagcatcag gtttttgagg tcgtcgttga
     4681 cgtctttgta ccggtctttc ttaaggacat tgttcacggc cgtcgtccag aggatctgcg
     4741 accctggagt cacgctccac cagttgccca gctccacctg gaccttcggc agttcatcct
     4801 gaaggatgcg cggcatcagg tgcaggaatt ctcccttaac ggcctgctca aaactcgacc
     4861 ccgtcgcccc gccaggcagt ttatgggtct ggaccgtggg gtcgaaaccg agaaactggg
     4921 attcaaaatc gcggtagtat tcccgctccg cccgtagtct gttggaagtg tcgatgatcg
     4981 cctggcggtc tatacccatt gcatggtgcc cgtagtcctc gagggtgtcg aggatcgtca
     5041 gaagcggcgg ggggccgaaa aaccccgtga tcgaatggtc accgccgtcg acgatctttg
     5101 caccgtttct aaccgcctcc acggtccggc cgatgtcgtt gccgtccgtg ttatgcccgt
     5161 gataatggat gattaggtcc gggaatttct gcagaatcgc gttcaccagc tctcctatcc
     5221 gcctttgcgt gccgagtccc ccgtgattct tgatacaaag gatgatgtcc tccccagctg
     5281 cttcgattat ctcgcctact ttacggacat agaattcgtt cgtatgttcc gggccggtgg
     5341 agaaacagat agaaggttcg aggatgcatc ccgctgcctt gacctcctcg aaaaccggga
     5401 tcatgttggg gacgtaattc aaaaaatcga agacacgcca tacctggatg taacgggcga
     5461 agctcttgac ggtcaatctg atgacgttct ccggatacgg cctgtaaccg aagaggttga
     5521 ctcccctgca cagggtctga aacatcgtgt ccggcatgct cttgctcaat atctccagtt
     5581 tctccagggg actgatctgt ttcctgagca tgtccacgtg gacggaggcg ccgccggata
     5641 tctcgagcga gaaaaatccc gccgcgttcc tttcctccgc aacgagaatc tgggtgtggg
     5701 gcatgatgcg gttcttgaaa tcggattgag aaaggtccct ctccgtgttg gtaacataaa
     5761 aaccccttgc cttcctgagc cgctcgaaaa catggctaac tcctttttcc cggaactcgg
     5821 aaggtgtgat ccgttcgtcg attatttctt gcggtatctt catagcctta aactccctag
     5881 gctgaaactc ttatttgctc cgatgtaatc tttcttgtcg ctaagccgcg tatgggttgt
     5941 gtttcctggc gtgtatctcc gctatcagtc gtgcgagcct cgcgatttcc ctttcctgct
     6001 ccgggtagtc gaatatctcc ggatgggtat ccaaatagct agtgtcgaag acgccgcgcc
     6061 tgaagtccgg ttcgtcacag atggccaggt agaaggggat tgtcgtcttc ggtcccacga
     6121 tcaggaagtc attgagcgcc cgtttgagcc tctgcacgac cgcttcccac tgataccccc
     6181 gaacggtcag cttcaccagc agggaatcgt agtccatggg aatattgtag ccctggtaca
     6241 cgatgccgtc gagccgtatt cccggtccgc ccggagactg ataaacctcg accctttttc
     6301 ctccctcggg catgaagttg tttttcgggt cttcggcgtt aatgcgcac
//
LOCUS       DAOTMX010000061         6269 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_10710_length_6269_cov_3.179755, whole genome shotgun sequence.
ACCESSION   DAOTMX010000061 DAOTMX010000000
VERSION     DAOTMX010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 6269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6269
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10710_length_6269_cov_3.179755"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1004
                     /locus_tag="P5573_02770"
     CDS             <1..1004
                     /locus_tag="P5573_02770"
                     /inference="COORDINATES: protein motif:HMM:NF024732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HRT27023.1"
                     /translation="EIRDDYAWCEAGRCMYLPIDDVDYFIEVDTSQEKYQWGYLAEKN
                     IKPRDVETAIANNCLSIMRDGDCIQVGIGALPTAVVIALRDSNLRHLGVHSEMIGEYA
                     FTLTEAGVVDNSRKTIDRGRCGWAYIMPVDTPRYYEWLHRNPYFAGYDIGYTNNILTL
                     SRIDNMVAINNFAQIDLRGHDAASNVGGRPISSAGGQFSFTLGAAMARGGRAIIAATA
                     RDAKGRSRFVPMLDQGSTVDVPDWLVTWVCTEFGIVNIQGCTDAEKARRLISIAHPDD
                     REGLERAAVEKLNIKSNHWMFNVAPDRRFPTPEEQKDHKYGYMDVEVRPGQIAKEND"
     gene            1886..2482
                     /locus_tag="P5573_02775"
     CDS             1886..2482
                     /locus_tag="P5573_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPR1/FUN34/YaaH family transporter"
                     /protein_id="HRT27024.1"
                     /translation="MAHEHSFANPGPSALGALAVACFGFGAVFLGKVAVEGLPLLAAW
                     LFGGCLVQYTAGVIELKDHQIAGGNVFVFFSAFFMLAAGVSTLSKFFMIKFGMKPAVH
                     VEGWMWMAGALFLTLVTPAYAKSTSILFLVVILVDIALWCIVGLDSGWYGDPASFKQI
                     VAWCLIISGFLGCYIAGAVVCNTVYGKPVFWLPKPLVS"
     gene            complement(2645..3829)
                     /locus_tag="P5573_02780"
     CDS             complement(2645..3829)
                     /locus_tag="P5573_02780"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HRT27025.1"
                     /translation="MRDAVIVEAVRSPGGRYRRGGLAATRAEEIGIQVVKGLMSRVPV
                     LKPEDIDDLIVGCAFPEAEQGMNLGRVIALGARLPLRTSGMTINRFCSSGLQSIADAT
                     AKIRAGWSDVVIAGGCETMSHIPMGGSVFRPHPDWGDRPNTYVSMGITAENVAARYGM
                     AREDLDRFGVESNRRAYEAIKAGKFKDEIIPIEAWAYKKNNGKRVREKVVFDMDDGVR
                     WPTSMEEMARLKPPFKQGGVVTAGNSSQMTDGAAFSLLMTDEKAKELGLKPLARLAYY
                     AVEGCAPEEMGVGPAVAVPKVLRLAGLKTDDIDLFEFNEAFAAQALYCIRALGLEERY
                     KAGDVNPNGGAIALGHPLGATGAKLTAQIIYEARRRKAKRCIVTMCIGGGMGAAGIFE
                     IL"
     gene            complement(3849..>6269)
                     /locus_tag="P5573_02785"
     CDS             complement(3849..>6269)
                     /locus_tag="P5573_02785"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HRT27026.1"
                     /translation="MPFEIKKAAVLGAGVMGAAIAAHLTNAGVECVLLDIIPPEFTEE
                     DKKKGLTEKSPAWRNRFAQNGLAAILKAKPAAFYTKKNASMVKTGNFEDHLEWLADCD
                     WVIEAVVENLEIKRGLLARVEKVVNDRCIVSTNTSGIPIRDIAANLNPKLKERFLGTH
                     FFNPPRYMKLLELIPGEQTKKEVIEFMVKFCEDVLGKGVVICKDVPNFIANRIGVFDI
                     SNAIHIMLEKNLRVEELDAIIGKSLGRPGSAIFGTLDLVGLDTGYHVMRNLYEAVPDD
                     EGRSYFMPSEFMQKMMERKWLGNKTRQGFYKKTTDAAGNKVKLVLDYRTMEYVPPTSP
                     KYESLASAKKKEGTFADKLKVIFYGDDIAAEVAREYLINNFIYAANRIPEICDNIVAI
                     DNAMKWGYNHKLGPFEMWDAVGLEESVALMKKMNKHVPKKVEDMLKAGFTSFYQKRAD
                     GLYYFDFTTMGYVRLPENPKVILLPSLKERNRLVKRNAGASLLDIGDGVACLEFHTKM
                     NAVDQDVIQMIFDACDVVENDDFVGLVVGNHAQNFSVGANIFMVLVAVQKGDWDVLEK
                     LVADFQNANMRMKYLAKPVVSAPAGLTLGGGCEIAMHAARCQPCGETYIGLVEVGVGV
                     IPSGGGTKELMVRMTEGIPDGAVEQGLNLQQYYSKVFETIGMAKVATSAAEAMELGYV
                     RKTDGISLNRDHQLWDAKQVVLGLSRFYKKPRPAMVPVMGENLRGMIDVILSNMRQGN
                     FISDYDVHVGKKLAYVISGGDCPEGTPVSEQEILDLEREAFLSLLGEAKTQDRIMHML
                     TTGKPLRN"
BASE COUNT         1274 a         1717 c         1807 g         1471 t
ORIGIN      
        1 gtgaaatccg cgacgattac gcctggtgcg aggcgggaag atgcatgtac ctccccatcg
       61 acgacgtaga ctacttcatc gaagtggaca caagtcaaga gaaataccag tgggggtatc
      121 tcgccgaaaa gaacatcaag ccgagggacg tcgagacggc tatcgcgaat aactgcctct
      181 cgataatgag ggacggcgat tgcatccagg tcgggatcgg cgccctcccc acagccgtgg
      241 tcatcgccct gagagactcg aacctcaggc atctcggtgt ccacagcgag atgatcggcg
      301 agtacgcctt cacgctcacc gaggccggcg tcgtagacaa ctctcggaag accatcgaca
      361 gagggagatg cggctgggca tacatcatgc ctgtcgacac gccccgttac tacgaatggt
      421 tgcatcggaa cccctacttc gccggatacg acataggcta cacgaacaac atccttacgt
      481 tatcccggat cgacaacatg gtcgccatca ataacttcgc ccagatcgac ctccggggac
      541 acgacgcggc gagtaacgtc ggcggacgtc ccatctcctc ggcgggcggg cagttctcgt
      601 tcaccctcgg cgccgcgatg gccaggggcg ggagggccat catcgcagca acagctcggg
      661 atgccaaggg taggtcccgt ttcgttccga tgttggatca agggagcaca gtggatgtcc
      721 ccgactggct cgtgacctgg gtctgcacgg aattcggcat cgtcaacatc caaggctgca
      781 cggacgccga gaaggcacgg cggctcatct cgatcgccca tcccgacgac agggagggcc
      841 tggagagggc ggccgtcgag aaactgaaca tcaagtcaaa tcactggatg ttcaatgttg
      901 cccccgaccg tcggttccct acccctgaag agcagaagga tcacaagtac ggctacatgg
      961 atgtcgaggt gcgcccgggg caaatcgcca aagaaaacga ttgattgtta ggtcgttaga
     1021 gtgatttggg aaattggaga ggcaaggtaa tatcaccttg cctctttcca ttggggcgct
     1081 cggcatgggc gtgcagctcg gaatgaaagt tcccgcgggt cctgcatgct cgacataagg
     1141 gcaaatgaca aggccggttg aacacgaagg ccttttacgt tgacaagcgt cgtcagcctg
     1201 aatataatca caatgctttt catgactgcg attttcttgg ggatctctta tggcgagggg
     1261 aaagaaaaaa aagacggcgc ccttaaggca gaatatcgaa atgctccctc cgccacggtc
     1321 ctctggcagg tcaaggtacc agtggtgggc cttcgtcata tgggtcgtgg gtgtcatctt
     1381 gatactgata aaatggggca atttttgagg attcagtcgc atgagaagcg ggaagttatt
     1441 ttccccttga caaggggaag tcgattttgc tattcggtta aaaagtttgt tgcagtaaag
     1501 gttctttgaa aaaaaggaga tagcgccata gcacgagaaa gggccctgac aactgaatct
     1561 ttcaggtcca aagtctgacg cgctcgtgaa acaccctaaa aggtagggta ggagaaacta
     1621 aagaaagctg ctcgattcgg gtgagatcca aagcacataa taaactggat tcgacccggg
     1681 ggtgactggt tggaatgggg gaaaagattt tccgactttt tacgaggtcg tcatagttcg
     1741 atggagagcg ctcgagcaat cgggtgcacg ataccagaga agggggggat gccgggcgta
     1801 gtgaacggaa aatagagggg gtcaaatagt ctattaatca agttctttat catttccgtt
     1861 ttttaggaaa aaaggaggag tcattatggc gcatgagcac agtttcgcaa atccggggcc
     1921 ttccgcattg ggagcgctcg cagttgcttg cttcggtttt ggtgcagtat ttcttggtaa
     1981 agtagcggta gaaggtctgc cgctcctcgc ggcctggttg tttggtggat gccttgttca
     2041 gtacacggcc ggcgttatcg agctcaaaga tcatcagatc gccggcggta acgtatttgt
     2101 tttcttctct gccttcttta tgctcgccgc gggagtgagc accctgagca agttcttcat
     2161 gatcaagttc gggatgaagc ctgcggtaca cgtcgagggg tggatgtgga tggccggtgc
     2221 tttgttcctg accctggtta ctcctgcgta cgcgaagagc acttcgatcc ttttcctggt
     2281 agttatactc gtcgacatcg cactctggtg catcgtcggt ttggacagcg gttggtatgg
     2341 tgacccagcg tcattcaaac agatcgtcgc ctggtgcttg atcatcagcg gattcctcgg
     2401 ttgttatatt gcgggggcgg tggtgtgcaa cacagtttac gggaaacccg tcttctggct
     2461 tcccaaaccc ctggtcagct agattgaatt gagttgatgt tatggtcgca agggcaggct
     2521 gttgaaagac agcctgccct tttttatgga gttctgtccg gggacggaaa ataaatgaga
     2581 gggccgtgat ccaaaatcgg tttcacggcc ctctcattgt tgaagaaggg gtaagggctg
     2641 ctttttacag gatttcgaag atgccggcgg cacccatgcc gccgccgatg cacatggtga
     2701 cgatacatcg cttcgccttg cggcgtcggg cctcatagat tatctgggcc gtgagtttgg
     2761 cgcccgtagc accgagggga tggccgaggg cgatagcgcc gccgttcggg ttgacgtctc
     2821 cggccttgta gcgttcttca agtcccagcg cccgaatgca gtagagggct tgggcggcga
     2881 aggcctcgtt gaactcgaag aggtcgatgt cgtcggtctt gagccccgca agtcggagga
     2941 ccttgggtac ggcgacggca gggcctacgc ccatctcttc cggcgcgcag ccttcgacgg
     3001 cgtaataggc aagcctagcc aggggcttca acccgagttc cttcgccttc tcgtctgtca
     3061 tcaacagaga gaaagcagcg ccgtccgtca tctgcgaaga gtttcccgcc gtgacgacac
     3121 ctccttgctt gaacggtggt ttgagccttg ccatctcctc catggatgta ggccagcgga
     3181 caccgtcgtc catgtcgaag acgacttttt cgcggacccg cttcccatta ttctttttgt
     3241 aagcccaggc ctcgataggg atgatctcgt ccttgaattt tcctgccttt atggcctcgt
     3301 aagcccgacg gttgctctcc acgccgaacc tgtccaggtc ttctcgtgcc atcccgtacc
     3361 tcgccgctac gttctccgca gtgataccca tggacacgta ggtgttgggg cggtcacccc
     3421 agtcgggatg gggacggaag acgctcccgc ccatggggat atggctcatc gtctcgcagc
     3481 cgccggcgat gaccacgtcc gaccacccag cccgtatctt ggccgtcgcg tcggcgatcg
     3541 actggagccc cgacgagcag aaacggttta tggtcatgcc ggaggtacgg agcggcaacc
     3601 tggctccaag ggcgatgacc ctcccgagat tcattccctg ttccgcttcg ggaaaggcac
     3661 agccaacgat gaggtcgtcg atgtcttccg gcttgaggac ggggaccctc gacataagcc
     3721 ccttcacaac ctggatgccg atctcctcgg ccctcgttgc ggcgagacct cctctcctgt
     3781 aacgtcctcc cgggcttctt actgcttcaa cgataacagc atcgcgcatg ataatcctcc
     3841 ttattagatc aattacgcaa gggtttgcct gtggtaagca tgtgcatgat acggtcctgg
     3901 gtcttagctt cgcctagcag actcaggaag gcctctctct ccaggtccag tatttcctgc
     3961 tcggagaccg gcgttccctc ggggcaatcg ccgccggaaa tcacgtaagc cagtttcttc
     4021 ccgacgtgga cgtcgtaatc ggagatgaag tttccctgcc tcatgttgga gaggatgacg
     4081 tctatcatgc ccctgaggtt ctcgcccata acgggaacca tcgccggtct cggtttcttg
     4141 tagaatcggg agaggcccag aacgacctgc tttgcgtccc agagctgatg gtctcggttc
     4201 aggcttatcc cgtcggtctt cctcacataa ccgagttcca tagcctccgc ggcgctcgtc
     4261 gccaccttgg ccatcccgat ggtctcgaag accttggagt agtactgctg taaattgagt
     4321 ccctgctcga cggctccgtc ggggatgccc tccgtcatcc tgaccatcag ttccttggtg
     4381 ccgccgccgg agggaatcac gcccactccg acctcgacga gtccgatgta ggtctcgcca
     4441 cagggctggc atctcgcggc gtgcatggcg atctcgcatc cgccgccgag cgtcagcccg
     4501 gcgggggcgg agacgacggg tttggcaagg tatttcatcc gcatgttggc gttctggaag
     4561 tccgctacga gcttttccag gacatcccag tcacccttct gcaccgccac cagcaccatg
     4621 aatatgttgg cgccgacgga gaagttctgg gcgtggttgc cgacgaccag cccgacgaag
     4681 tcgtcattct ccacgacgtc gcaggcgtcg aatatcatct gaatgacatc ctggtccacg
     4741 gcgttcatct tggtgtgaaa ttccaggcag gccacgccgt cgccgatgtc gaggaggctt
     4801 gccccggcgt tccgcttgac caaccggttc cgttctttca gagagggcag gaggatgacc
     4861 ttgggattct ccgggagcct cacgtatccc atcgtcgtaa aatcgaaata gtagaggccg
     4921 tcggccctct tttggtagaa ggaggtgaag ccggccttca gcatatcttc gaccttcttg
     4981 gggacgtgct tgttcatctt tttcatcagg gcgacggatt cttccaaccc cacggcgtcc
     5041 cacatctcga agggacccaa cttgtggttg taaccccatt tcatggcgtt atcgatcgcc
     5101 actatgttgt cgcagatttc ggggatgcgg ttggcggcat agataaagtt gttgatgagg
     5161 tactcccggg cgacctccgc ggcgatgtcg tcaccgtaga agatgacttt gagcttgtcg
     5221 gcgaaagtgc cttctttttt ctttgccgag gcgagggatt cgtatttcgg cgatgtcggg
     5281 ggtacatact ccatcgtgcg ataatccagg acgagcttta ctttgtttcc cgcagcgtcg
     5341 gtcgtttttt tgtaaaagcc ctgcctcgtc ttgtttccga gccatttcct ttccatcatc
     5401 ttctgcatga attcactggg cataaaatag cttcgccctt cgtcgtccgg tacggcctca
     5461 tagaggttcc tcatgacgtg ataccccgtg tcgagcccga ccaggtcgag agtgccgaaa
     5521 atggcggatc cgggccttcc cagcgattta ccgatgatgg cgtcgagctc ttcgacccgg
     5581 aggttctttt ccagcatgat gtgaatggca ttcgagatgt cgaagacccc gatccggttg
     5641 gcgatgaagt tgggaacgtc cttgcagata acgacgccct tgccgaggac gtcctcgcag
     5701 aacttgacca tgaattcgat gacttccttc ttcgtttgct cgccggggat gagttcgagg
     5761 agcttcatgt agcggggcgg gttgaagaag tgggtgccga ggaatcgctc tttgagcttg
     5821 gggtttaagt ttgcagcaat atcccggatc gggataccgg aggtgttggt gctgacgatg
     5881 cagcggtcgt tgacgacctt ctcgaccctg gctaaaagac cccgtttaat ctccaggttt
     5941 tctacgaccg cctctatcac ccagtcgcaa tcggcaagcc attcgagatg atcctcgaag
     6001 ttgcctgtct tgaccatcga ggcgttcttc ttcgtgtaga aagcggcggg tttggctttc
     6061 aggatcgctg ccagcccgtt ctgggcgaag cgattccgcc aggcaggact cttttccgtc
     6121 agcccctttt tcttgtcttc ttctgtaaac tcagggggga tgatatccaa caggacacat
     6181 tcgaccccgg cgttagtcag gtgcgccgcg atggcggccc ccatgacacc ggctcccagt
     6241 acggcggctt tcttgatttc gaaaggcat
//
LOCUS       DAOTMX010000062         6188 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_10876_length_6188_cov_3.795858, whole genome shotgun sequence.
ACCESSION   DAOTMX010000062 DAOTMX010000000
VERSION     DAOTMX010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 6188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6188
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10876_length_6188_cov_3.795858"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..950)
                     /locus_tag="P5573_02790"
     CDS             complement(<1..950)
                     /locus_tag="P5573_02790"
                     /inference="COORDINATES: protein motif:HMM:NF023222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HRT27027.1"
                     /translation="MALKTKEEYFESIRALRLPVYLLGERVKDTVEHPIIRPSLNSVG
                     MTYEIAQDPLYEDLATATSHLSGNKINRFCHIHQSIDDLQKKPRLLRLMGQKTGSCFQ
                     RCAGLDAINTLNAISYEMEQELGTEYHQRFLNFLRIMQDEDLVCDAALTDPKGDRSLP
                     PSKQADPDLYLRIVKKDAQGITVRGCKAHQTGAVNSHWVAVTPTSAMREEDKDYAVTF
                     VAPTNSKGISYVYGRQSCDTRKLEGGNIDVGNAGFGGHEAYMIFDDVFVPWENVLMCG
                     EYQFSGRLVEIFANYHRASYGGCKTGVGDVLIGATALIAD"
     gene            complement(1413..1488)
                     /locus_tag="P5573_02795"
     tRNA            complement(1413..1488)
                     /locus_tag="P5573_02795"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1453..1455),aa:Ala,seq:ggc)
     gene            1592..1679
                     /locus_tag="P5573_02800"
     tRNA            1592..1679
                     /locus_tag="P5573_02800"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1626..1628,aa:Leu,seq:caa)
     gene            2090..2653
                     /locus_tag="P5573_02805"
     CDS             2090..2653
                     /locus_tag="P5573_02805"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HRT27028.1"
                     /translation="MSAGKRAFSRLGVPLVHLGVSIMIIGALLGAMLGFDADIHIVEG
                     ETVDEVPLVGKEKTINLGFSVRLDRFYMDFYDDGTPKTYRSDLTFMKEGRVIFQGPLF
                     VNHPISLEGLRFCQSGYMTTGGDSGLTSARGGEDRYVSELRVTRDHGAGIVAAGAAVM
                     MVGFVLVLFHILSMARKEKASHEDIGR"
     gene            2650..3492
                     /gene="ccsB"
                     /locus_tag="P5573_02810"
     CDS             2650..3492
                     /gene="ccsB"
                     /locus_tag="P5573_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HRT27029.1"
                     /translation="MTISTTLLGIVAFIYLLAFLLYVLDMVRRAGFWGDAAITTAAVG
                     FTIQTIAIALRWEESYSLGFGHAPLANLYESLVFFAWTVVLFHFLVEWRTRSRSPGVF
                     TMPVSFFLIAYASFHPGVDSSIRPVVPALQSNWLVCHVVACFFGYAAFTIACGVSLML
                     ILKERVGQDDGDSFLSLLPGPGFLDETIYRSAFLGFILFGLGIVSGSVWAYNAWGSYW
                     SWEPKEVWSLITWLIYATMLHLRFVHGWKGKRTAWVALVGFASVLFTYLGVNYLPGLH
                     SYLH"
     gene            complement(3506..5104)
                     /locus_tag="P5573_02815"
     CDS             complement(3506..5104)
                     /locus_tag="P5573_02815"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HRT27030.1"
                     /translation="MLQFMRKHAKNWLMKVLLILIIIVFIFYFGSSTGRQKAGDIATV
                     GDKTISFVEFRKEYENLLELYRRQYGENLTEEQLKRLNLKQVAVDNLINQAVILQKAM
                     DLNIEVSDEEVRNFIFSIPTFQRNGVFDNRVYQQVLRSLKMTPEEFEVSQKMGMIAAK
                     IEGLIRESVMVSDDEAFDIYRIQNEKTDLAFLKIPCEGYRSRVKASTEDLEKFLAEQG
                     DSFRVPERLQVKYLFLRAEDFEDSVSVSDEEVRDYYESHRESFKKKGGSAAPLAEVKG
                     KIIAALRRDKAMDLAYREAKKARNTIYQEENFEEYARKHNITIHSGEVTAKANIPPEL
                     AAIEGLDQYLSALKPDQITPVLSTPSGYAIIKLVSRESSRTPKLAEIRGIVERRYVEE
                     EARKLCRKEAEDTLRRLKAGVGMDEIAREKGLRIGDTGYFSPGVNIPGIGRSLEIAQA
                     VYLLSEKKPYPDQPFLVDDSYVIVRFKGRKMEAKDFDANKAAIKQFLYRHKENIYLQS
                     WIAETKEALIKAGRIKFHEDVSKL"
     gene            5339..>6188
                     /locus_tag="P5573_02820"
     CDS             5339..>6188
                     /locus_tag="P5573_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRT27031.1"
                     /translation="MLFDFILGKFSNDLAIDLGTANTLVYVKGKGIVLSEPSVVAVHR
                     DSRGVQKVLAVGADAKRMLGRTPGNIVAIRPMREGVIADFEITEAMLRHFIMRVHNRR
                     TLVRPRIIISIPSGITQVERRAVKETAESAGAREVYLIEEPMAAAIGAGLPITEPLSS
                     MVVDIGGGTTEVAVISLAGIVYSKSVRIAGDRMDDFIVQHMKRKYSLLIGERTGEMIK
                     TAVGCAYPDGEIRTVEVKGRDLISGIPKTIEIDSEEVREAIAEPVSIIVDAIKDALEQ
                     CPPELAA"
BASE COUNT         1335 a         1519 c         1691 g         1643 t
ORIGIN      
        1 taatcggcaa tcagggcagt tgctcctatc aggacatctc caacaccggt cttgcaaccg
       61 ccgtagctgg cccggtgata attggcgaag atttcgacaa gcctcccgga aaactggtac
      121 tcaccgcaca tgaggacgtt ctcccaagga acgaagacat cgtcaaaaat catgtaggct
      181 tcgtggccgc cgaaaccggc gttccctaca tcgatgtttc cgccttcaag ctttctcgtg
      241 tcgcaggact gacgaccgta gacgtaagag atacccttgc tgttcgttgg ggccacgaaa
      301 gttacggcgt aatccttgtc ctcctcgcgc atagccgacg tcggggtaac cgctacccag
      361 tgagaattga ccgcaccggt ctgatgggcc ttgcatccac ggacggttat cccttgggcg
      421 tcctttttca caattcgcaa atagaggtcg gggtccgctt gcttgctggg cggcaaggat
      481 cgatcaccct ttgggtccgt gagggcggcg tcacagacaa ggtcttcatc ctgcatgatt
      541 cgtaagaaat tgaggaacct ctgatggtat tccgtgccca gctcctgttc catctcatac
      601 gagattgcgt tgagggtatt gatggcatcc agtccggcac aacgctggaa acaggagccg
      661 gtcttctgcc ccatcaaccg cagcagccgc ggcttcttct gaaggtcatc tatactctgg
      721 tgaatgtgac aaaaccgatt gatcttgttt ccgctcagat gggatgtagc ggtagccaaa
      781 tcctcataga gggggtcctg ggcaatttca taagtcattc caacggaatt cagcgaagga
      841 cgtattatcg gatgctctac agtgtctttc actcgttcgc ccaatagata taccggcaac
      901 ctcaacgccc ttatgctctc aaaatattct tcctttgtct tcagagccat acttaatcct
      961 ccttttaggt tgctgatgaa cgcactgtaa aatctccagt gcattgtctg tttatccaga
     1021 agcaaatagc aggccaccgg acgaccggcg cgcctgggaa tggataatac attgattatc
     1081 ttaccaatac ggcgattgaa gtcttgtttt atggttcgat agaaaatgac tattcggtaa
     1141 gcggggaggc gtcgctgcta tgagtcaagt ttacaacagc ttggcggtga aagacaaagg
     1201 atgtaacgat cgctataaac agtagttaaa tcaatatatt gctccgaatg ggtgtcagcc
     1261 gccctttttt tgtgctgcct gtgcagaaac gcacagaatt gtgcgtaagt gcacagtata
     1321 taggcagaga gtttttgtgg actgcgaatc atgagagaat gatagcgtga gaaaagaaaa
     1381 ggacctaacg aaaaagttaa gcccttatgc tatggtggag ctgacgagga tcgaactcgt
     1441 gacctcttga atgccattca agcgctctcc cagctgagct acagccccac gtaaaatgcc
     1501 tgaaaggtga cgaagtttaa tcagagactc cacaaattgt caatacattt ttcttgacag
     1561 gaaaggtgcc cgaatataat ctcggaccca tgccggagtg gtgaaattgg tagacgcagg
     1621 ggactcaaaa tcccccgacc cttgcggtca tgacggttca agtccgtcct ccggcaccat
     1681 gaaatcgaaa ggttacgagt cttgatccgg gctcgtaacc tttttttatc cgatcggagt
     1741 tatgagattc gaatgttcct ttacgaaaga gagactgatt tatggtagat tctcttttgt
     1801 tgatttcatg tactgagcgg gagtgctgtg aggatgaggc gtcgcgttga aggatgagcc
     1861 ggataagaat agggtggtag ggcggaacgc gctttggttt ttcttgacct ccgtcaggcc
     1921 ggccgttgcc ttgatctgtt tggtcgccct ttgttcaggt ttcggggcgg tcgtgccgtc
     1981 tgtcaatgtc tatcattctt gggggttttt tcttcttctc ggactccttt ccttgaatct
     2041 tttgatttgc tcgtggcgcg ggttttcggg tttcgggggg tgttttcgca tgagcgccgg
     2101 gaagagggcc ttttcccggc ttggagtccc tctcgttcat ctgggcgtct ccatcatgat
     2161 catcggcgct ctgctcgggg caatgcttgg ttttgatgcc gacatccaca tcgtcgaagg
     2221 ggagacggtc gatgaagtgc cgctcgtggg caaggagaag acaataaatc tcggcttttc
     2281 ggtccgcctc gaccgctttt acatggattt ttatgatgac ggaacgccta agacgtaccg
     2341 ctcggacctc accttcatga aagaaggccg tgttattttt caaggcccgc ttttcgtgaa
     2401 tcatcccatc tcgttggaag gactgcggtt ctgccagtca ggttatatga cgacaggggg
     2461 agactcaggt cttacatccg caaggggagg agaggatcgg tatgtctccg agctgcgggt
     2521 cactcgtgac cacggtgcgg ggatagttgc cgcaggggca gccgtcatga tggtggggtt
     2581 tgtgctggtc ttgttccata ttttgagtat ggccaggaaa gagaaggcat ctcatgagga
     2641 tattggacgg tgacgatcag tacgacgctt ctgggcatag tcgcattcat ttatctcctg
     2701 gcttttctgc tgtatgtcct cgatatggtg cggagggcgg gcttctgggg tgatgcggcg
     2761 attacaacgg cggccgtggg attcaccatc cagacgattg ccatagccct gagatgggaa
     2821 gagtcttata gtctcggttt cggacacgcc ccactcgcca acctttatga gtcactcgtt
     2881 tttttcgcct ggacggtcgt tctcttccat ttcctcgtag agtggagaac gagaagcagg
     2941 agtccggggg tgttcaccat gccggtgagt ttctttctca tcgcatacgc atcctttcac
     3001 cctggagttg atagcagcat tcggcctgtg gttcctgcgc ttcagagcaa ctggcttgta
     3061 tgtcatgtcg tggcctgctt tttcgggtat gccgctttca ccatcgcctg cggcgtcagc
     3121 ctcatgttaa ttttgaagga gagggtaggg caggacgacg gggattcttt cttgagtcta
     3181 ttgcccgggc cgggctttct cgacgagacg atctatcgga gcgcgtttct tgggttcatt
     3241 cttttcggcc ttgggatcgt ctcaggctca gtctgggctt acaacgcctg gggctcttac
     3301 tggagttggg aaccgaagga agtctggtca ctcatcacat ggcttatcta cgccacgatg
     3361 ctacatctgc gatttgtgca tggctggaag ggaaagcgga cggcgtgggt ggcgctggtc
     3421 gggtttgcct cagtgctttt tacctatctg ggagtgaact atctgcctgg ccttcacagc
     3481 tatctgcatt agccctttgc ccggtttaca gtttggagac gtcttcgtgg aacttgatcc
     3541 gtcctgcctt tataagggcc tctttggtct cggcgatcca cgattggagg tatatgtttt
     3601 ctttgtgccg gtaaaggaac tgtttgatgg ccgccttatt ggcgtcgaag tccttcgctt
     3661 ccattttgcg gcccttgaat ctgacgatta cgtatgagtc gtccacgagg aagggctggt
     3721 ctgggtaagg ttttttctcc gaaagcaggt acacggcctg tgcaatttca agggacctac
     3781 ctatcccggg gatgttgact cccggcgaga agtacccggt gtcgcctatt cttaggccct
     3841 tttcacgagc aatctcgtcc atgccgacgc ccgctttcag gcgcctcagg gtatcttccg
     3901 cctctttccg gcagagtttc cgcgcttctt cctcgacata acgcctttcg acgatgcccc
     3961 ttatctctgc gagtttcggg gtccgggaac tctctcggga gaccagtttg atgatcgcgt
     4021 aaccagatgg agtggagagg accggtgtga tctggtccgg tttgagggct gagaggtatt
     4081 ggtccagtcc ctcgatggcg gctaattccg ggggaatgtt tgcttttgcc gtgacctctc
     4141 ccgaatggat cgtgatgtta tgcttcctcg catactcctc gaaattctct tcctgataga
     4201 tagtgttgcg ggccttttta gcctccctgt aggcaaggtc catcgccttg tcgcggcgta
     4261 aggcggcaat gatttttccc ttcacttcgg cgagtggggc cgcgctgccg ccttttttct
     4321 taaaggattc cctatgagat tcgtagtaat ctctgacctc ctcgtcggaa acgctaacgg
     4381 aatcctcgaa gtcctcggcg cgaaggaaca agtacttgac ctgcaagcgc tcgggtacac
     4441 ggaaagagtc accctgttcg gcaaggaact tttctaggtc ttcggtcgat gctttgacgc
     4501 gggaacggta cccctcacag ggaattttta ggaacgccag gtctgtcttt tcattctgga
     4561 tacggtagat gtcgaaggcc tcgtcgtcgg aaaccatcac ggattcccgg ataagacctt
     4621 caatcttcgc cgcgatcatt cccattttct gtgaaacttc gaattcttcc ggcgtcatct
     4681 tcaggctgcg caggacctgc tgataaacac ggttgtcaaa tacaccgttt ctctgaaatg
     4741 tcggtatgga aaaaatgaaa ttacggacct cctcgtcgct gacctcgatg ttcaagtcca
     4801 ttgccttctg gaggataacg gcctggttga tgaggttatc aaccgccacc tgcttgaggt
     4861 ttaatctttt aagctgctct tcggtcaggt tttcaccata ctgccggcgg tatagctcga
     4921 gaaggttctc gtattccttc cggaattcga caaaggatat cgttttatcc cctaccgtcg
     4981 cgatgtcccc cgctttctgt cggccggtac tcgaaccgaa atagaagatg aagacgatga
     5041 tgataaggat cagaagaacc ttcatcagcc agtttttcgc gtgttttctc atgaattgca
     5101 gcatgaatgg accgccgtcg ttcctttttg ttaactgtag aaaatcacca tctattttcc
     5161 ccgtctaccg gcagaattaa gtagtatatc gcccggcaaa aagcaatgtt tttttaaagg
     5221 caaatgattt tttgtactta aaaaatgaat tgattagccc gagtaaataa gttataaaaa
     5281 tcagctttca ataccttgag ttattataag cctaagttgt aaaaataagg aggcaatcgt
     5341 gctttttgac tttattctgg gtaaattttc caacgacctg gcgatcgacc tcggaacggc
     5401 aaacaccctt gtctatgtca agggcaaggg catcgtgttg agcgaaccct ccgtagtcgc
     5461 cgttcatcgt gattcgaggg gtgtccagaa ggtgctggct gtcggggccg acgccaagcg
     5521 catgctcggc cgaacccctg gaaacatcgt cgccataagg cccatgaggg aaggggtcat
     5581 cgccgacttt gagataacag aggccatgtt gcgtcatttc atcatgcggg tccacaaccg
     5641 gagaaccctg gtccgtccga ggatcatcat ctcgatacct tccgggatca cgcaggtgga
     5701 gagaagggcg gtgaaggaga cggccgaatc ggcaggcgct agagaggttt acctgataga
     5761 agagcctatg gcggcggcga tcggggcggg tctacccatc acggagccgc tcagttcgat
     5821 ggtagtggac atcggcggag gaacgacgga agtggccgtc atatcccttg caggtattgt
     5881 gtattcgaaa tcggtgcgca ttgctggtga caggatggac gacttcatcg ttcagcacat
     5941 gaagagaaag tacagcctcc ttatcggtga gagaacaggt gagatgataa aaacggcggt
     6001 gggctgcgca taccccgacg gtgaaatccg cacagtggaa gtcaagggtc gggacctcat
     6061 atcaggcatc cctaagacta ttgagatcga ttcagaggag gtcagggagg ctatcgctga
     6121 gcccgttagc atcatcgtgg acgcgataaa ggacgcctta gaacagtgcc ccccggaatt
     6181 ggcggcgg
//
LOCUS       DAOTMX010000063         6170 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_10908_length_6170_cov_3.692723, whole genome shotgun sequence.
ACCESSION   DAOTMX010000063 DAOTMX010000000
VERSION     DAOTMX010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 6170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6170
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10908_length_6170_cov_3.692723"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="P5573_02825"
     CDS             complement(<1..656)
                     /locus_tag="P5573_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HRT27032.1"
                     /translation="MIYNEEAETLPREALKALQLKRLQQVVHRVYHTVGFYRRAFDQA
                     GVDPDDIKSLDDLQKLPFTTREDLRNNYPFGLFAVPMSNVVRLHASSGTTGKATVVGY
                     TRRDIENWSELVARCFTAAGVTKNDIIHNAYGYGLFTGGLGAHYGAEKLGASVIPMSG
                     GSTKRQITILQDFGPTAICCTPSYALTIADAGTELGVDMRSLKLRVGVFGAEPWSEKM
                     "
     gene            complement(671..1780)
                     /locus_tag="P5573_02830"
     CDS             complement(671..1780)
                     /locus_tag="P5573_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HRT27033.1"
                     /translation="MGQDIRIAIVGIGNCASALVQGIEYYRDQKGQEDTTGLMHWDIG
                     GFKPFNIEVVAAFDVDERKVGKDLSEAIFQLPNCTTVFHRNIPRKGVTVRMGRILDSI
                     APHLSDYEEKLRFCVAREPEPSREDVVRILKDSGAEILLNYLPVGSEKATGFYAECAL
                     EAGIALINNIPVFIASDPAWSDRFAKKGLPVIGDDIKSQLGATIVHRVLTNLFKNRGV
                     KLERTYQLNIGGNTDFLNMLDRSRLASKKESKTEALQSQVNRRLSNENIHIGPSDWVP
                     WQKDNKIAYLRMEGRLFGDVPMNLELRLSVEDSPNSGGVVIDAIRCCKLALLRGQGGV
                     LYSPSAYFMKHPPKQSSDDEAYRMTEEFIKGTRAN"
     gene            complement(1962..3026)
                     /gene="rfbB"
                     /locus_tag="P5573_02835"
     CDS             complement(1962..3026)
                     /gene="rfbB"
                     /locus_tag="P5573_02835"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HRT27034.1"
                     /translation="MSRSFENLLVTGGCGFIGSNFIRHLLLKRSDFRGRVINIDKLTY
                     AGNRENLKEIEQICGDRYAFIRADICDLDTMNRIISEYAVDGICHFAAESHVDRSIKR
                     PADFILTNIVGTFNLLEAARLRKDRFLLFHHISTDEVYGSLGPEGMFTEKTPYRPNSP
                     YSASKASSDHLVRAYHETYGLPVTLSNCSNNYGPYQFPEKLIPLTIINALDFKPLPVY
                     GDGRNVRDWLYVEDHCEAIWAIMKSGPAGETYNVGGGTEMENIAVVRMICDLVDEISA
                     SPGGKSRRDLITFVKDRPGHDRRYAMDFSKLNRELGWRPKESFRSGLQKTIHWYLDNR
                     DWIQRVRTGDYYSWLKEQYG"
     gene            3173..6157
                     /locus_tag="P5573_02840"
     CDS             3173..6157
                     /locus_tag="P5573_02840"
                     /inference="COORDINATES: protein motif:HMM:NF019966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HRT27035.1"
                     /translation="MVAEGCPAPLFKAGSKVRGFRVIRVEEIPDVRATAYLIEHEATG
                     ARVIHLHTDDRENLFSVGFRTPPRDSTGVAHILEHSVLAGSERYPVKDAFNELVRGTL
                     QTFINAFTYPDKTVYPVASRVRKDFFNLARVYTDLVLRPRLLRETFLQEGHHLEFLDS
                     DNLESDLTISGIVYNEMKGVHSSADALMWKSLQEALLPDTVYAFDSGGNPEVIPYLTY
                     EQLVEFHRTYYSPSNAYFFICGDIPTDEHLSFLEEMLDGFGRTDVDSGIEKQPRWERP
                     VRVSSTYPIAREEDTAGKTSVNVAWLLADNTDSETILLLRILSEALVGGAAGPLRKAL
                     IESGLGEDMTPVTGLETDLRQAVFAVGLRGTDPDKADAIEEVVLGTLRGIVADGFERD
                     IIEGALHQVEFEGKEIVRRQYPYAIKLMRKVYQTWLYGGDPFSNLKFGTAIDSIRDRL
                     TREKDLFERLLKEWFLENPHRVLSVLEPSKTHNEEWDRTFNAMMRHCKENLSQAEKED
                     IRRETEMMKVRQVEADSEEALGTLPKLRTEDIPREVEMIPSEETLVDGVPVVVHDVYS
                     NGIAYINVAFDVSDIPDELQVLLPLLGKLTTDMGAAGLSYTEMAKRIALKTGGIDCRL
                     EAGLTADGRGDWQKMVFSARCLDRNIRETLAILADLLCAGDLSDTGRIRDLIFEKRNR
                     LYASVIPSGHLFARRTAAAPISVPAYREEQWYGRTQLQVLNGLVDNFDDDPRRIFDRM
                     CELREWVFKKSRLQVDLTADEKTLKDLLERVPGLIDRLADGRGVVALPVPQRQALNKG
                     ISIPAKVCFVARVFPAPPYKDRLAPSLMVLSRELSNNHLYKRVRLQGGAYGGMSVYDA
                     MAGYLAFLSYRDPNLEETLKVYDDAIKIAARGDFKESDIEKAKVGTIGLIDRPMDPAT
                     KGYTAMVRRFAGITDKDRQAYRDAVFESSLASVKEAAERHLSAAVEGSSTAVYAPLER
                     LQSANEKLEETPLEVEPLTSPA"
BASE COUNT         1438 a         1593 c         1721 g         1418 t
ORIGIN      
        1 ctcatttttt cactccaggg ttcggcgccg aagacgccga ctcgaagctt gagggacctc
       61 atatcgacgc ccaattcagt tcctgcatcc gcgattgtca aggcatacga cggggtgcaa
      121 cagatcgccg tcggtccgaa atcctggagg atggtgatct gtcttttcgt gctgccgccg
      181 gacatgggta tgacgctggc cccgagtttt tcggcgccgt agtgggcgcc aaggccgccc
      241 gtgaagaggc cgtacccgta agcattgtga attatgtcgt ttttggtgac accggcggcg
      301 gtaaagcagc gcgcaactaa ctcggaccag ttttcgatgt cccgtctcgt ataacctacg
      361 accgtggctt tgccggtcgt gccggatgag gcatggagcc tgacgacgtt gctcatcggg
      421 acggcgaaga ggccgaaagg atagttgttc ctcaagtcct ctctggtcgt gaaggggagt
      481 ttctggaggt catcaagaga cttgatgtcg tcagggtcga cacccgcctg gtcgaaagcc
      541 ctgcggtaaa aacccaccgt gtgataaacc cggtgcacga cttgctggag tcttttgagt
      601 tgaagggcct tcagggcttc tctgggcagg gtttctgctt cttcattata tatcatgtgt
      661 tctccttgaa tcagttagcg cgtgtgcctt tgatgaattc ctccgtcatc cggtaagcct
      721 catcatcgct ggactgtttt gggggatgtt tcataaaata ggcagacgga gagtaaagaa
      781 cgcctccctg tcccctcaga agggcaagct tgcagcagcg aatggcgtca atgacgacac
      841 cgcctgaatt aggtgagtcc tcgacggaca agcgtaactc aaggttcatc ggaacatcgc
      901 caaaaagcct tccttccata cgcaagtaag ctattttgtt gtctttttgc catggcaccc
      961 agtcgctcgg cccgatgtgg atgttttcgt tcgacagccg gcggttgacc tgcgattgga
     1021 gcgcttccgt cttcgattct ttcttcgaag cgaggcgtga ccggtcgagc atgttcaaaa
     1081 agtcagtgtt gccccctatg ttgagctgat acgtccgttc cagcttaacc cctctattct
     1141 tgaaaaggtt ggtgaggacg cggtggacga tagttgcgcc caactgggac ttgatatcgt
     1201 ctccgattac cggaaggcct tttttggcga agcggtcgga ccatgccggg tcgctggcga
     1261 tgaaaacagg gatgttgttg atcaaggcta taccggcctc gagggcgcat tccgcgtaaa
     1321 atccggtggc tttctcggaa ccgactggga gatagttgag aagaatctcg gctcccgaat
     1381 ctttgagtat ccgcacgacg tcttctcggg aaggttccgg ctcacgggcg acgcaaaatc
     1441 tcagtttctc ctcataatcc gaaagatgag gtgcgatgct gtccaagatg cgtcccattc
     1501 gcaccgtaac gccctttcgc gggatattcc gatgaaaaac cgtcgtacaa ttcgggagct
     1561 gaaagattgc ctcggaaagg tctttcccga cttttcgctc gtcgacgtcg aaagcggcaa
     1621 cgacttcgat gttgaaaggc ttgaaacctc cgatgtccca gtgcatcaac cccgttgtgt
     1681 cttcttggcc tttttggtcg cgatagtatt caatcccttg gacgagggcg gaagcacagt
     1741 tgcctattcc tacgatcgct atccgtatat cctgacccat agaaaatcaa ccccctgaaa
     1801 attattgtca tgccgattgt taggactgaa agcaggttgt gatggaaaat aaaaaagcaa
     1861 tgttaagtat atacatataa taagaatcgg ccgataattc aaatataatt tataatccat
     1921 gatggaccga cgaatgtcgg tcagactcgg gaccggccga gtcaaccgta ctgctctttc
     1981 agccaagaat aatagtcccc ggtccggact ctttgaatcc aatctcggtt gtccagatac
     2041 cagtggatcg tcttctgtag accggaacga aaggattctt tgggacgcca gccgagttct
     2101 cggttgagtt tagaaaagtc catggcgtat cttcggtcgt gacctggtcg gtccttcacg
     2161 aaggtgatca agtctctgcg agatttgccg ccgggcgatg ctgaaatttc gtcgaccagg
     2221 tcgcaaatca tcctgacgac ggcgatattc tccatctctg tgccgcctcc gacgttatac
     2281 gtctcccccg ccgggccgga cttcattatg gcccagattg cctcacaatg atcctcgaca
     2341 taaagccaat ccctcacatt ccgaccgtct ccgtaaacgg gaaggggttt gaaatcaagg
     2401 gcattgatga tggtcagggg aatcagcttt tccgggaact gataagggcc gtaattgttc
     2461 gaacagttcg aaagggtgac cggaaggccg tatgtctcat ggtacgccct gacgagatga
     2521 tcggaagagg cctttgaagc ggaatagggg ctgttgggac ggtaaggcgt cttttccgtg
     2581 aacattccct ccggtccgag acttccgtac acttcatcag tgctgatgtg atggaagagt
     2641 aaaaaacggt ctttccggag tctggcagcc tcgagaagat tgaaggtgcc gacgatgttg
     2701 gtaaggataa agtcggccgg tctcttgatg gaccggtcga catgggattc ggcggcaaaa
     2761 tggcatattc cgtcgacggc atactcggag attatccggt tcatcgtatc gaggtcgcag
     2821 atgtcggccc tgatgaatgc ataccgatca ccgcaaatct gctcaatttc cttcagattt
     2881 tctctgttcc cggcataggt cagcttatcg atgtttatca ccctgccccg aaaatcggac
     2941 ctcttgagga gaagatgacg gatgaaattg ctcccgatga aaccgcatcc ccctgtaacg
     3001 agaaggtttt cgaacgacct tgacaccgct tccattctat cccctgacag acgctaagat
     3061 cttgcaatga caagttaatt ctgtcaaact tatgcattca agaagctgac gttatttaac
     3121 aaataaaccg ataaaataat agtattattt tttcttggga gaggaaacga acatggttgc
     3181 agaaggatgt ccggcgccac ttttcaaagc cggctcaaag gttaggggtt tcagggtcat
     3241 aagggtagaa gagatcccgg atgtccgggc tacggcatac ttgatcgaac atgaagcaac
     3301 gggtgcccgg gtcatccatc tgcacaccga tgaccgggag aacctttttt ccgtgggatt
     3361 tcggacacct ccgcgagact cgacaggcgt cgcccatatt cttgagcatt cagtccttgc
     3421 cgggtccgaa cggtatcccg tcaaggacgc cttcaacgag ctggtaaggg ggacgctgca
     3481 aaccttcatc aatgccttta cctacccgga caagaccgtt tatcccgttg ccagccgggt
     3541 aagaaaagac ttcttcaatc ttgcccgtgt ttacaccgac ctggtgctcc gaccgaggct
     3601 gctgagggag acattcttgc aggaagggca ccacctcgag tttctcgata gcgataattt
     3661 ggaaagtgac ctcaccatat cgggtatcgt gtacaatgag atgaaagggg tccattcctc
     3721 cgccgatgcc ctgatgtgga aatcgctcca ggaagccctt ctgcccgaca cggtctatgc
     3781 cttcgactcg ggaggaaacc ccgaagtcat tccttacttg acctatgaac agcttgtcga
     3841 attccaccga acctactatt cgccgtcgaa cgcctatttt ttcatctgtg gtgacatccc
     3901 taccgacgaa cacctttcct tccttgagga gatgctcgac ggattcggga ggacggacgt
     3961 cgattcaggg atcgaaaaac aaccccggtg ggaaagaccg gtacgagtca gctctaccta
     4021 tccgatagcc cgggaggagg atacggcggg aaaaacctcg gtcaatgttg cctggttgct
     4081 cgcagataat accgacagcg agaccatcct ccttctcagg atcttgtcgg aggcgctcgt
     4141 cggaggcgct gccggaccgt tacgtaaggc actgatcgag tccggtctgg gtgaagatat
     4201 gacgcctgtg accggactcg aaaccgacct tcgtcaggcg gtgtttgccg tcgggttgag
     4261 gggcaccgac ccggacaaag ccgatgccat agaggaggta gttcttggca ccctgagggg
     4321 catcgtggct gatggattcg agcgtgacat catcgagggt gcgctccatc aggtcgaatt
     4381 cgaaggaaaa gaaatagtca ggcgtcaata tccctacgcg ataaaactga tgcggaaggt
     4441 ctatcagacg tggctctatg ggggcgaccc tttttcgaat ttgaagttcg ggactgcgat
     4501 cgacagcata cgcgatagat tgacccggga aaaggacctc ttcgagaggt tgctcaaaga
     4561 gtggtttctc gagaatccgc accgagtgct ctcggttctc gaaccgagca agactcacaa
     4621 tgaagaatgg gatcgaacgt tcaacgccat gatgaggcac tgcaaagaga acctgtcgca
     4681 ggcggaaaag gaggacatac gccgggagac ggagatgatg aaggtccgtc aagtcgaggc
     4741 ggactcggaa gaggccctcg gcacgcttcc caagttgcgg acagaagaca tcccgcggga
     4801 ggtcgaaatg ataccgagtg aagagaccct cgtggacggc gtaccggtcg tggttcatga
     4861 cgtatattcg aacggcatcg catacataaa cgtggctttt gacgtctccg acatccccga
     4921 cgaactgcaa gttctcctcc cgctcctcgg gaaactgacg acagatatgg gagccgccgg
     4981 cctttcctat acggagatgg ctaaacgcat cgccctcaaa acgggcggaa tcgattgtcg
     5041 gctcgaggcg ggactaacgg cagacggccg gggggactgg cagaagatgg tattcagcgc
     5101 ccgttgttta gacaggaaca tccgggaaac tctcgcgatt ctggcagacc tgctctgcgc
     5161 cggtgatttg agcgatacgg gtaggattcg ggatctgata ttcgaaaagc gcaaccgatt
     5221 gtacgcatcg gtaatcccct caggacatct gtttgcccga aggacggcgg ctgccccgat
     5281 atccgttcct gcctaccggg aagaacagtg gtacggccgc acgcaacttc aggttctgaa
     5341 cggccttgtc gataattttg acgatgaccc gcgccgaatc tttgacagga tgtgtgagct
     5401 gcgggaatgg gtgttcaaaa aaagtcgcct ccaggtcgac ctgaccgccg acgaaaagac
     5461 gttgaaggac ttgctggaac gtgtacccgg actgatcgac cgccttgccg acgggcgtgg
     5521 ggtcgttgcc ttgccggttc cgcagagaca ggcactcaac aaagggattt cgataccggc
     5581 gaaggtttgt ttcgtggccc gggtattccc ggctccgccg tacaaagacc gtcttgcgcc
     5641 gtctctgatg gtcctcagca gggagctatc gaacaatcat ctttacaaaa gagtgcggct
     5701 gcagggaggc gcttatgggg gcatgagtgt ttatgatgcg atggcgggat acttggcctt
     5761 cctttcttat agggacccta atctggaaga aacgctgaag gtttacgacg atgcgataaa
     5821 gatcgccgcc cgcggtgact ttaaggagag cgacatagaa aaagcgaagg tcggtactat
     5881 cggactcatc gacaggccca tggacccggc cacgaaaggc tatacggcca tggtaaggcg
     5941 cttcgcaggc atcacagaca aggatcgaca ggcgtaccgt gatgcggtct tcgaatcgtc
     6001 gcttgcatcc gtcaaggaag ccgccgaacg acacctgtcc gctgcagtgg agggttcatc
     6061 tacggccgtc tatgcgcccc tggagaggct tcaatcggca aacgaaaagc tggaagagac
     6121 gcccctggag gtcgaaccgt tgacctcacc agcgtgagaa tattcccttg
//
LOCUS       DAOTMX010000064         6127 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_11006_length_6127_cov_4.452404, whole genome shotgun sequence.
ACCESSION   DAOTMX010000064 DAOTMX010000000
VERSION     DAOTMX010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 6127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6127
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11006_length_6127_cov_4.452404"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1415)
                     /locus_tag="P5573_02845"
     CDS             complement(<1..1415)
                     /locus_tag="P5573_02845"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRT27036.1"
                     /translation="MVEEAVGRLKEDPRYSGRVVHVEVLPQKGPAWGTLARELPESLS
                     RFLEKKKIKLYSHQAEAVEAIREGRNVIITTPTASGKTLAYNLPVFEGLLQDPAATAL
                     YLYPTKALANDQLNTLREMDKAAGVDARPAVYDGDTPAAKRPGIRERSRVVLSNPHEL
                     HQVLPWHHKWRRFFSNLRFVVIDEAHRYRGVLGSNTAYLIRRLRRIAHAYGSRPQFVL
                     SSATLANPVEFGERLVGLPFALIDRDGAPKGRKFFIFYNPYFDGAGTLSAHQETRDLF
                     LFFTRRRLQTLCFTMSRRMAELIARWSKKGAHEADPDLAGRIAAYRAGYLPEERRELE
                     DRLKRGDLIGVTSTNALELGIDVGSLDCVILSGYPGTVISTRQQAGRSGRGTDDSAAV
                     LVAFENPLDQYLMKHPGVFFGGSPENAAVSLENPYILSGQLLCAAAEMPLRPEEDGTF
                     LGDDIGDILKALEEEGLLKETP"
     gene            complement(1409..2476)
                     /locus_tag="P5573_02850"
     CDS             complement(1409..2476)
                     /locus_tag="P5573_02850"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HRT27037.1"
                     /translation="MSSTLDRIKEKWGNRADRAEEQEIAEESGFESVFAAALTGEEYE
                     EARRLKERLLRAYRGVSLEEAVPGQALTTGRGACYHVRTEEPFDLRVIGREKAREKLI
                     SDLKLIYGIGWVTERALKEKGYSTLEDLAGHPRFGERAEALLKIVDGGNTDRLYKWIG
                     RWFSRSHPLLLHTSGLHEKEDFLILDIETLGLFNRPIILFGAAQVSGGGIRVDQYLVR
                     DIGEEPAALAALLAHVGEHCAFVTFNGRAFDVPFIKERLAYYRMSGDLDKVHFDVLPF
                     SRRAWRDRAPNCRLTTLEAYLFGIEREDDVPSALVPEFYESYRRTGNVGPLIPIVRHN
                     RADLVTLARMFSKLHEEWGGW"
     gene            2720..4213
                     /locus_tag="P5573_02855"
     CDS             2720..4213
                     /locus_tag="P5573_02855"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HRT27038.1"
                     /translation="MQVILAGFNLDHEVISDLRRNDPDAAALTPETISAAYARISRNP
                     LPVNELRRIARKEVDAARQSNRNIVFEMGHSSVAEHAVFNIDVIGVSRLLVEEVEKFR
                     LCSYTEKSQRYVLLKDDFVVPEEIRRSGLEESFVDTVRLQNSFYHELYEALKVYVFEK
                     NRDLASDPANHSILEGWAKEDARYVISLATETQLGMTLNARNLELMLRRLAAHPLEEA
                     RECGRRLFDSVKDVAPSLVRYTEATAFDSRTRTLLREKAQGFVEKWGKKKWEDKGTRV
                     LAKRGNVSLVYATPDADERTVAGLLHSSSGLPMERCMVIASLMEREEKEEMIGAACRY
                     MEAYDAPLREFEYADLYFELVVSATCFAQLKRHRMATVVAQDYDPGLGVVIPQSVQAV
                     NMEGSFRRVIARTEETYEKVRKVDPVAAAYILTNAHQKRVILKVNAREFYHMVRLRAD
                     RHAQWDIRDTVERMLSLGRRVMPLTLMLATGKDGFPALHRKCFPHNP"
     gene            4256..5524
                     /locus_tag="P5573_02860"
     CDS             4256..5524
                     /locus_tag="P5573_02860"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRT27039.1"
                     /translation="MVEYNPLLGIFLFIFVAAAVTRWVLNSLNIGHLRRHGYEVPEVF
                     RGELDEETLSRMTAYTVESSRLSSVESLVGDAVLLAVLLSGVLPWFASLIESLKLHPV
                     LSGLVFFGTLALGGVLLEVPFSLYGTFGIEKRYGFSTITPRLWLTDFAKNLIISAVLM
                     ALLLGPLLAMIHYFPQAWWFLSWLFFALFQLLLLWLYPVVIAPLFNKYEPVKDESLKG
                     RIVELMEKVGLKVKGVYQVDASRRSRHSNAFFTGLGKTKRIVFFDTLLTTHSPDEILS
                     VLAHEVGHWVKKHILRQLLLVGAVSFAFFYICYLLLDWPRLYEAFGFEARLPYVGLFL
                     AGVTLKAVFFFFTPAGAARSRSFEREADQYAYSLVGTARPLVEALKRLAKENLANIHP
                     HPFYAWFYYSHPPLTERIATLLTLEGEKGP"
     gene            5590..6084
                     /locus_tag="P5573_02865"
     CDS             5590..6084
                     /locus_tag="P5573_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRT27040.1"
                     /translation="MPIYEFYCKKCNTVFSFFSKTVNTEKVPYCPRCKQVKLERRMSL
                     FAKVTRGKGEEPPEDMPPIDEARMEKAMAMLANEADGMNEDDPRQAARLIRKLSDATG
                     LDLGPGMEEALSRMERGEDPEKVEEEMGDILESEDPFSFAGTKPKGGSRKPKPRVDET
                     LYDL"
BASE COUNT         1154 a         1887 c         1860 g         1226 t
ORIGIN      
        1 ggcgtttcct tcaggaggcc ctcctcctcg agagccttca agatgtcccc tatgtcgtcg
       61 ccgaggaagg tcccgtcctc ttccggccgg aggggcatct cagccgccgc gcagagaagc
      121 tgtccggaga ggatgtacgg gttttcgagg gagactgccg cattctcggg ggacccgccg
      181 aagaataccc ccgggtgctt catcaggtac tggtcgagcg ggttttcgaa ggccaccagg
      241 acggccgccg agtcatccgt cccccggccc gaccgccccg cctgctgccg ggtcgagatc
      301 accgtcccgg ggtaaccgga aaggatcacg cagtccaggg aacccacgtc gatgccgagt
      361 tccagggcgt tggtggaggt gacgccgatg aggtcgcccc gcttcaggcg gtcctccagt
      421 tcgcggcgct cttccgggag atagcccgcc cggtaagcgg cgatccggcc ggcgaggtca
      481 gggtccgcct cgtgcgcgcc tttcttcgac caccgggcga tgagctccgc catccgcctc
      541 gacatggtga agcagagggt ctgcaggcgg cggcgggtga agaagaggaa gaggtcgcgc
      601 gtctcctggt gggcggagag ggtcccggcc ccgtcgaagt agggattgta gaagataaag
      661 aattttctcc ccttcggggc gccgtcccgg tcgatcagcg cgaaagggag ccccacgaga
      721 cgttcgccga attccaccgg gtttgcgagg gtcgccgacg aaaggacgaa ttgcggtcga
      781 gagccatagg catgggcgat tctcctcagg cgcctgatga gataggccgt gttcgacccc
      841 aggacgccgc gataccgatg ggcctcgtcg atgacgacga accggaggtt gctgaaaaag
      901 cgccgccact tgtggtgcca ggggaggacc tggtggagct catgggggtt actcaggacg
      961 acccgcgagc gttccctgat gccgggcctc ttcgcggccg gcgtgtcgcc gtcgtagacg
     1021 gcgggcctcg cgtcgacccc cgcggcctta tccatctccc ggagggtatt caactggtcg
     1081 ttcgccagcg ccttcgtggg gtagagatag agggccgtgg ccgccgggtc ctgaagcagc
     1141 ccttcgaaga cggggaggtt gtaggcgagc gtcttccccg atgccgtggg ggttgtgatg
     1201 atgacattcc gaccctccct tatggcctcc acggcctcgg cctggtggct gtaaagcttg
     1261 atcttcttct tttcgaggaa ccgcgacaag gattcaggga gctctctcgc gagggttccc
     1321 caggcaggcc ctttctgcgg cagaacctct acgtgcacga cccgcccgct gtaccgcggg
     1381 tcttccttca gacggccgac ggcctcttct accatcctcc ccactcctca tggagcttag
     1441 aaaacatcct cgccagggtg acgaggtctg cccggttgtg gcgaacgatc gggatgagcg
     1501 gccccacgtt gcccgtccgg cggtagctct cgtagaactc gggcacgagc gcgctcggga
     1561 cgtcgtcctc gcgctcgatg ccgaagagat aggcctccag ggtcgtcaac cggcaattcg
     1621 gcgcccggtc ccgccaggcc ctccgcgaga aagggaggac gtcgaagtgg actttgtcca
     1681 ggtcgccgct catccggtag taggcgagtc tctccttgat gaagggtacg tcgaaggccc
     1741 ttccgttgaa ggtcacgaag gcgcagtgct cacccacgtg ggcgaggagg gcggcgaggg
     1801 cggcgggctc ctcgccaata tcgcggacca ggtactggtc cacgcggata ccgccgccgg
     1861 aaacctgcgc cgctccgaag aggatgatcg gccggttgaa gagccccagg gtttcgatgt
     1921 cgaggatcaa gaagtcttct ttttcgtgga ggccggatgt gtggagcagc agtggatggg
     1981 accgggagaa ccatcgtccg atccacttgt aaaggcggtc cgtattgccg ccgtcgacga
     2041 tcttcaagag cgcctccgct ctctcgccaa aacgggggtg gccggcaagg tcctcgaggg
     2101 tgctgtaccc cttctccttg agggcccttt cggtaaccca gccgatcccg tatatgagct
     2161 tcaggtcgga gatgagtttc tcccgcgcct tctcacgacc gatcaccctg aggtcgaagg
     2221 gctcttccgt cctcacgtgg taacaggcac ccctcccggt ggtaagggcc tgtcccggga
     2281 cggcctcttc caggcttact ccgcggtaag ccctgaggag tctttctttg agcctccgcg
     2341 cctcttcgta ttcttctccg gtcagcgcag cggcaaaaac ggactcgaaa ccgctttcct
     2401 cggcgatttc ctgttcttcc gcccggtcgg cacggttacc ccacttttct ttgatacggt
     2461 caagcgtaga gctcacggtt ccctcacttc cggccgacca tcataacatc cccgccggcc
     2521 tatcctgcaa tacgatgagg cgtgtttccg ttctaccact tttcacggca acggcaaagg
     2581 acttccgggc ttccaactgg acggcggggt ttttccccct cagtcacgag ggcgtcggcc
     2641 gcaagcggtt tgaccactgt gccgattcct gctatagaac agcttatcat gaattttcac
     2701 aaggtcgtga ggagttcttg tgcaggtaat actcgccggg ttcaacctgg accatgaagt
     2761 cataagcgac ctccgccgca acgaccccga tgcggcggcc ctcacgccgg agacgatttc
     2821 cgccgcttac gcccgcatca gccgaaaccc cctgccggtg aatgagctgc ggcggattgc
     2881 ccgcaaagaa gtggatgcgg cgagacagtc caaccgcaac atcgtcttcg agatgggtca
     2941 cagctccgta gccgagcatg cagtgttcaa catcgatgtc atcggcgtgt cgaggctcct
     3001 cgtcgaagag gtcgagaagt tccgtttgtg ttcctacacg gaaaagtccc agcgctacgt
     3061 gctcctgaag gacgatttcg tcgtccccga agagatccgc cggtctggcc tcgaagagtc
     3121 cttcgtcgac accgtccgcc tccaaaacag tttctatcac gaactttacg aggccctgaa
     3181 ggtctacgtc ttcgaaaaaa accgcgatct ggcgtcggac cccgccaatc actccatctt
     3241 ggagggatgg gccaaggaag acgcccgtta cgtcatctcg ctcgccactg agacccagct
     3301 cgggatgacg ctgaacgcca gaaacctcga gctgatgctg aggcggctcg cggcccatcc
     3361 cctggaagag gcgcgggaat gcggccgcag gctcttcgac tccgtgaagg atgtggcccc
     3421 ctctctggtg cggtacaccg aggcaacggc cttcgactcc cgaacgcgga cgctcctcag
     3481 ggaaaaggcg cagggttttg tcgaaaagtg gggcaagaag aaatgggagg ataagggcac
     3541 ccgggtcctg gcaaaacggg ggaacgtttc cctcgtctac gccacacccg acgccgatga
     3601 gaggacggtg gccggtctgc ttcactcctc gtcgggcctt cccatggagc ggtgcatggt
     3661 gatcgcgtcg ctcatggaga gggaggaaaa agaggagatg atcggggccg cctgccgtta
     3721 catggaggcc tacgacgcgc cgttgcggga gttcgagtat gcggacctct atttcgagct
     3781 cgttgtgagc gccacctgct tcgcccagtt gaagcggcac cggatggcca cggtcgtcgc
     3841 ccaggattat gacccgggcc ttggcgtggt gatcccgcag tcggtgcagg ccgtgaacat
     3901 ggaagggtcc ttccggcggg ttatcgcccg gacggaagag acctacgaaa aggtgagaaa
     3961 ggtcgatccc gttgccgcgg cctacattct gaccaatgcc catcagaagc gcgttatcct
     4021 gaaggtaaac gcaagagaat tctatcatat ggtacggctc cgggcggacc gccatgccca
     4081 gtgggacatc agggacaccg tggagaggat gctcagcctc ggcagaaggg tcatgccgct
     4141 taccctcatg ctggcgacgg gcaaggacgg tttccctgcc cttcaccgta agtgcttccc
     4201 ccataaccct tagtgcgatt tcccgaagat agagtgaccg accgaaggaa gacagatggt
     4261 cgaatacaac ccccttctgg ggatatttct ttttatattc gttgccgccg ccgtaacgag
     4321 atgggtcctg aacagtctca atatcggtca tctccgccgg cacggttacg aggtgccaga
     4381 ggtcttccgg ggagaactcg acgaagagac cctctcgcga atgacggcat acacggtcga
     4441 gtcctcccgg ctgagttccg tcgagtcctt ggtcggcgac gccgtcctgc tggcggtcct
     4501 cttgagcggt gtccttccct ggttcgcgtc cttgattgaa tccctaaagc tccaccctgt
     4561 gctgtccggt ctcgtgttct tcgggacgct cgccctggga ggggtgctgt tggaagtccc
     4621 cttcagcctg tacgggacat ttgggatcga gaagcgctac gggttcagca cgataacccc
     4681 gcgactctgg ctgaccgact tcgccaagaa cctcatcatc tccgccgtcc tgatggcgct
     4741 cctcctcggc ccgctgctcg cgatgatcca ttacttcccg caggcctggt ggttcctgag
     4801 ctggctcttt ttcgccctat tccagttgct cttgctatgg ctctatccgg tcgtcatcgc
     4861 ccctcttttc aacaagtatg aacccgtgaa ggatgaatcc ctgaaaggac ggattgtcga
     4921 gctcatggaa aaggtggggc tcaaggtcaa aggcgtatac caggtcgacg ccagccgcag
     4981 gagcaggcac tccaacgcct tcttcacggg gctcgggaag acgaaacgca tcgtcttctt
     5041 cgataccctt cttacgaccc acagccctga cgagatcctt tcggtgctcg cccacgaggt
     5101 ggggcactgg gtgaagaaac acatcctgcg gcagcttctc ttggtcggag cagtgtcatt
     5161 cgctttcttc tacatctgct atctccttct cgactggccc cggctctacg aggccttcgg
     5221 attcgaggcc cgcctcccct acgtgggcct tttcctggcc ggtgtgacct tgaaggccgt
     5281 ttttttcttc ttcacgccgg cgggcgccgc ccgttcccgc tccttcgagc gcgaggccga
     5341 ccagtatgcc tattccctcg tggggacggc gaggccgctc gtggaggccc tgaaaagact
     5401 ggcgaaggaa aacctggcaa acatccatcc ccatcctttc tacgcttggt tctactactc
     5461 ccacccgccg cttacggagc gcatcgccac gctcttaacc ctcgaggggg aaaaaggtcc
     5521 ttgatttttt ccgccgaatt tttttaaatg tttcgcactc gaaccttgaa tcttaaaatg
     5581 gttttaacca tgccgatcta cgagttttac tgcaaaaagt gcaacaccgt cttcagcttt
     5641 ttctccaaga ccgtgaacac ggaaaaggtc ccgtactgcc cccggtgcaa acaggtaaaa
     5701 ctggagcggc ggatgtccct tttcgccaag gttacccggg gcaaggggga ggagcccccg
     5761 gaagacatgc cccccatcga cgaggcgagg atggaaaagg ccatggccat gctcgcaaac
     5821 gaggccgacg gcatgaacga agacgacccc cgccaggccg ccaggctgat aaggaaactg
     5881 tccgacgcca cgggcctcga tcttggcccc gggatggagg aggccctgag ccggatggag
     5941 aggggggagg acccggaaaa ggtcgaggaa gagatgggcg acatactgga gtcggaagac
     6001 cccttttcct tcgccgggac gaagccgaag ggggggtcaa ggaagccaaa gccccgggtt
     6061 gacgagacgc tgtacgactt gtaaccggaa gcggttcttt ttttccatca accataacgc
     6121 cttggaa
//
LOCUS       DAOTMX010000065         6121 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_11016_length_6121_cov_3.663040, whole genome shotgun sequence.
ACCESSION   DAOTMX010000065 DAOTMX010000000
VERSION     DAOTMX010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 6121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6121
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11016_length_6121_cov_3.663040"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="P5573_02870"
     CDS             <1..768
                     /locus_tag="P5573_02870"
                     /inference="COORDINATES: protein motif:HMM:NF014792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HRT27041.1"
                     /translation="LPLPFVLVNKMMRAFENCGIPYFAPDGKGQVSVEYDDETGMPVR
                     TAKVLMSNAVDYRFAPKGGRKAIEGEAKKIAFESLGDWVDKKTQFLFNPTGEWRAVYS
                     CSSADSGVTGRKLVVQFYGGYPGAQVGGGSVVNKSPEKVDCSAAFGARYAAKNIVAAK
                     LARKCSIQLSYAIGVARPMSIYVNTFGTGALPDKKIAELVNNVFDLTPRGMIEHFDLL
                     NSEVYRRIPRTLFMDNYPWEKTDRVKELKNSAKALGR"
     gene            765..2021
                     /gene="ahcY"
                     /locus_tag="P5573_02875"
     CDS             765..2021
                     /gene="ahcY"
                     /locus_tag="P5573_02875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HRT27042.1"
                     /translation="MNYDIKDIKLAEEGRLSVEWAKKSMPVLETTRKRFEREKPLKGV
                     RLGACLHVTTETAALMETLKAGGAELALCASNPLSTQDHVAAYLVKDLGIPVFAVKGE
                     DTDTYYRHIHAVINIEPTLTMDDGADLVSTIHAERQDLTARIIGGTEETTTGVVRLRA
                     MAAKGVLRFPMVAVNDALTKHMFDNRYGTGQSTLDGIIRATNRLVAGSVFVVCGYGWC
                     SKGLAMRAEGMGANVIVTEVDPLKALEAVMDGFRVMTIDEAASLGDFFCTLTGNIHVI
                     RKEHFLKMKDGAIVANSGHFNVELDLEALGAMAVARRKVRSNIDEYDLGNGRKIYVLA
                     EGRLVNLAAAEGHPSSVMDMSFANQALAAEYLVKNGGKLENKVYPVPIDIDKEIARLK
                     LESMGVRIDKLTEEQERYLNSWEIGT"
     gene            complement(2052..3131)
                     /gene="lptG"
                     /locus_tag="P5573_02880"
     CDS             complement(2052..3131)
                     /gene="lptG"
                     /locus_tag="P5573_02880"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HRT27043.1"
                     /translation="MTILDRYVSKEFLKIFVLMMASFTLLYLIIDFFGRIRMFLSNHA
                     TAYQIAAYFIYMIPQIVSHTLPVAVLLATLLTFGMFSRSHEIVAMKANGLSLYRIALP
                     VFVIAALVSAVSFLFNEFVTPYANQRAKYIKFVEVQRTQKFGFFKQNQIWYRSRNAIY
                     NFGMFDPVADTLKGITIHYFDQNFSLVKRIDAREARWTDGHWSFKNVLTTSFPQGSIQ
                     VLERTPSAVIDIPEKPSDFKIVQKETDEMGYGELKNYIKKLRSEGYDVSRYLVDMHGK
                     ISFSVVSVILAVIGVAFSLRSERSGGPTASISVGIVIGFSYWIIFALALSLGRSLTLP
                     PLFAAWLPNILFAVAAVFMLSKVRT"
     gene            complement(3128..4300)
                     /gene="lptF"
                     /locus_tag="P5573_02885"
     CDS             complement(3128..4300)
                     /gene="lptF"
                     /locus_tag="P5573_02885"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HRT27044.1"
                     /translation="MRKILNRYILKEITLPFLMTLLVFTFVLLIGKILQIMDLMVNKG
                     VNILAVVKIVLFILPSFLTVTIPISLLIAVLIGLGRLSSDNEITAMKASGISLYQLLL
                     PIVTLCVVAMVATAVIGFLAPLSNSATKSLFFDILKEKASIGIKEKVFNDDFKGLVLY
                     ADRIPVEGNYMEGVMVFDNRLTKEPATIIAPQGYLSSNPESMTVTLRLLRGSIHSVDS
                     DLKTYKKTEFSTYDVNLDLQTALAEAKKASVKGSADMTVGELYRNIRKAEVKDDKLRD
                     MVIELNKKFAVPLSCLVFGIAAIPLGIVSRRSGKSRGFTVGLFVVAAYYTLLLAGEAF
                     GETGKIPPTVAVWSPNIIMGTLGIYLFVMAANEKPLPFSSPSDFTKNLYERLKGKK"
     gene            4430..5077
                     /locus_tag="P5573_02890"
     CDS             4430..5077
                     /locus_tag="P5573_02890"
                     /inference="COORDINATES: protein motif:HMM:NF004981.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase
                     domain-containing protein"
                     /protein_id="HRT27045.1"
                     /translation="MIDLHTHSIFSDGELIPAELARRAFVQGCRAIAITDHGDHSNVD
                     FIIPRIVKVCGKLSGAMPITVVPGIELTHIPPDVIGELAVECRRLGAKIVVVHGETIV
                     EPVAPGTNLAALNSSVDILAHPGLITEEEAKIAAKNGVYLEISTRKGHSLTNGHVLKT
                     ARLTGAELVLNTDSHAPQDIVGISMGRVIAAGAGMTDEEIERMFKNSAALLQKCV"
     gene            5077..5796
                     /locus_tag="P5573_02895"
     CDS             5077..5796
                     /locus_tag="P5573_02895"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HRT27046.1"
                     /translation="MKSVFLSDVHLRTERDRGYRRILNFLDGIRDEVERIFIVGDFFD
                     FWFCRNGSIYPPFREPVDKILELVERGVRISIFEGNHDFELAAYFGGKGIEVYPEWAD
                     FTLEGQRFLVSHGDTVDRSNYRYLALRKILRTGAFYRLQEMMPRPLLWQMASASSIVS
                     KGLTVESQKVLAEKMGAFAREKFEEGVDAVVLGHCHIPLLRQYEVKGRLKTFVTLGDW
                     VEHFSYLVFEEGRFELSFSES"
     gene            5920..>6121
                     /locus_tag="P5573_02900"
     CDS             5920..>6121
                     /locus_tag="P5573_02900"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HRT27047.1"
                     /translation="MNFGGIEARYAAFESAAFVVVPVPYDLTTTYQSGSRRGPQAILE
                     ASGNMELYDEELGGETYRAGIHT"
BASE COUNT         1403 a         1587 c         1831 g         1300 t
ORIGIN      
        1 ttgcccctcc cctttgtcct cgtcaacaag atgatgaggg ccttcgaaaa ttgcgggatt
       61 ccttacttcg cccccgacgg taagggccag gtttccgtcg agtatgacga cgaaaccggc
      121 atgcctgtgc ggacggccaa ggtcctgatg tccaacgcgg tcgattaccg tttcgcccct
      181 aaagggggtc gcaaggccat cgaaggggaa gccaaaaaga tcgccttcga gagtctcggc
      241 gactgggtgg acaagaagac ccaattcctt ttcaacccca cgggtgaatg gcgagccgtc
      301 tactcctgca gttcggccga ttccggagtg acgggccgca agctcgtcgt tcagttctac
      361 gggggttatc ctggtgccca ggtcgggggc ggatctgtag ttaacaagtc gccggaaaag
      421 gtggattgct ccgccgcttt cggcgcccgt tacgccgcga aaaacatcgt cgccgccaaa
      481 ctggccagga agtgttcgat tcaactttcc tacgcgatcg gggtcgcgcg gcccatgtcc
      541 atctatgtca acaccttcgg gacgggtgcc ttgcccgata aaaagatagc tgagctggtg
      601 aacaacgttt tcgacctgac cccgcgcggg atgatcgagc acttcgacct tctcaacagc
      661 gaggtctacc ggaggatccc gaggacgctg ttcatggaca attacccctg ggagaagacc
      721 gatcgcgtca aggaactgaa gaattcggca aaggcactgg gacgatgaac tacgacatca
      781 aagacataaa actcgcagag gagggcaggc tgagtgtcga atgggcgaag aaaagcatgc
      841 ccgtcctgga aacgaccaga aagaggtttg agagagaaaa acccctgaaa ggggtcaggc
      901 tcggggcgtg ccttcatgtg acgacggaga cggcggcgct catggagacg ctgaaggctg
      961 ggggcgccga gcttgccctt tgcgcctcca atccgctcag cacacaggat cacgtggcgg
     1021 cttacctggt gaaagacctg gggatccctg tctttgccgt aaaaggagag gacacggaca
     1081 cctattaccg tcacatccat gcagtcatca acatcgagcc tactctgacc atggacgacg
     1141 gcgccgacct tgtctccacg atccacgctg aacgacaaga cctgacagcg aggatcatcg
     1201 gcggtacgga ggaaaccacc acgggtgtag tccggcttag ggcgatggcg gccaagggcg
     1261 tgctgcgttt ccccatggtt gccgtgaacg acgccctgac gaaacacatg ttcgacaacc
     1321 gttatgggac agggcagagc accctcgacg gcatcatccg ggcgacaaac cgtctggtcg
     1381 cagggtcggt ttttgtggtc tgcggttacg ggtggtgttc gaaggggctc gcgatgcggg
     1441 ccgaggggat gggcgccaac gtcatcgtga cggaagtgga ccccctcaag gccctcgagg
     1501 ccgtgatgga cggcttcagg gtcatgacca tcgacgaggc cgcttctctg ggggactttt
     1561 tctgcaccct gacgggcaac atccatgtca tccgcaagga acattttttg aaaatgaaag
     1621 acggcgccat cgtggccaat tcgggacact tcaacgtcga actcgacctg gaagccctcg
     1681 gcgccatggc ggtggcccgc aggaaggtga ggagcaacat cgacgaatac gacctcggga
     1741 acggcagaaa gatatacgtc cttgccgagg ggcgattagt caatctcgcc gccgccgaag
     1801 ggcatccgtc gagcgtcatg gacatgagtt tcgccaacca ggcccttgcg gccgaatacc
     1861 tagtgaaaaa cggaggaaag ctggagaata aggtctatcc cgtgcctatc gatatcgaca
     1921 aggagatagc ccgtctcaag ctcgagagca tgggagtccg tatcgacaag ttgacggagg
     1981 agcaggaaag ataccttaac tcctgggaga tagggacgta ggggagggcg tccgttcttc
     2041 atgtgcctgc tttatgtcct caccttcgaa agcataaaga ccgctgcgac ggcgaagagg
     2101 atgttgggaa gccatgccgc gaatagcggc ggcaaggtaa gtgaacgacc gagggagagg
     2161 gcaagggcga atattatcca ataagaaaac ccgatgacga ttccgacact tatgcttgct
     2221 gttggccctc cgcttcgctc cgaccgcaac gagaatgcca caccgatgac ggcgaggatt
     2281 acgctgacga cggaaaaaga aattttccca tgcatatcga cgaggtaacg ggaaacgtcg
     2341 tatccctccg accgtagttt cttgatatag tttttcagtt ccccataccc catttcatcc
     2401 gtctccttct gtactatctt gaagtccgat ggcttttccg ggatgtcgat cacggcggaa
     2461 ggggtcctct ccagcacctg gatagagcct tgggggaagc tcgtcgtcag gacgttcttg
     2521 aaactccagt gcccatccgt ccaacgggcc tcccgggcgt cgatcctctt gacaagactg
     2581 aagttctggt cgaagtaatg gattgtgatc ccctttaggg tgtcggcgac ggggtcgaac
     2641 atcccgaagt tgtagatggc gttcctagac cggtaccata tctggttctg cttgaaaaag
     2701 ccgaattttt gagtcctctg cacctcgacg aattttatgt actttgccct ctggttggcg
     2761 tagggcgtca cgaattcatt gaaaagaaag gatacggccg agacgagggc ggcgatcacg
     2821 aacacgggga gggcgatccg gtagaggctc aatccgttgg ccttcatggc aacgatctcg
     2881 tggctccggg agaacatccc gaatgtcagc agcgtcgcga ggaggacagc aacgggcagg
     2941 gtgtgggaaa cgatctgggg gatcatgtag atgaaatatg cggctatctg gtaggccgtc
     3001 gcgtggttgc tcaggaacat ccggatcctg ccgaaaaagt cgatgatgag atagaggagg
     3061 gtgaaggagg ccatcatcag cacgaagatt ttcaggaact ccttcgaaac gtaacggtcg
     3121 aggatcgtca tttcttcccc ttgagacgct cgtagaggtt ttttgtgaaa tcggacggag
     3181 aggagaaggg aagcggtttt tcgtttgccg ccatgacaaa gaggtatatt cccagggttc
     3241 ccatgatgat gttcggagac cacacggcga ccgtgggtgg gattttcccc gtctctccga
     3301 aggcctcgcc ggcaaggagg agggtgtagt aggcggcgac aacgaaaagg ccaaccgtaa
     3361 accctctcga ttttcccgac ctcctggaaa cgatccccag gggaatggcg gcgatcccga
     3421 agacgaggca tgaaagaggg acggcgaatt ttttgttgag ttctatgacc atatccctga
     3481 gtttgtcgtc tttgacctcc gcttttcgga tgttccggta cagctctccg actgtcatgt
     3541 cggcgctccc cttcactgat gcttttttcg cttccgccag ggccgtttgg aggtcgagat
     3601 tcacgtcgta ggtggagaac tccgtcttct tgtaagtttt caagtcggaa tcgacggaat
     3661 ggatgctccc ccggagcagg cgtaacgtca cggtcatgga ctcggggtta gaactgagat
     3721 aaccttgcgg ggcgatgatg gtcgcgggtt cttttgtgag ccggttgtca aagaccatga
     3781 ccccttccat gtaattgccc tcgacaggta tcctgtcggc gtaaaggacc aaccccttga
     3841 aatcatcgtt gaacaccttc tccttgatac ctatgctcgc cttctccttc aggatgtcga
     3901 agaagaggct cttggtcgcc gagttactga ggggagccaa gaaaccgatg acggctgtcg
     3961 cgaccatcgc gacgacgcag agggtgacga tggggagcaa aagctggtag aggctgatgc
     4021 ccgaggcctt catcgccgtg atttcattgt cgctggacag tcttcccagg ccgatcagca
     4081 cggcgatcag gagcgatatg gggatcgtga cggtcaggaa tgagggcaag atgaacaaga
     4141 ctattttcac taccgcgagg atattgacac ccttgttgac catcaggtcc attatctgaa
     4201 ggatctttcc gatgagcagg acgaaagtaa agacgaggag cgtcatcagg aaagggaggg
     4261 tgatctcctt tagtatgtaa cggttcaaaa tcttacgcat aggcacttct tcgagttgcc
     4321 tctctatagc acactttaga agccggtgta aattatccta tctatacgtt gaatcgcact
     4381 gcaaattctg ttacataagc aaaaaaacac gattttcttt ggggcaccta tgatcgacct
     4441 ccacacccat tcgatattca gcgacggcga actgatcccg gccgaactgg ccaggagggc
     4501 cttcgtccaa ggatgcaggg cgatagcaat cacggaccac ggagaccact cgaacgtgga
     4561 tttcatcatc ccgaggatag tcaaggtctg cgggaagtta tcgggggcga tgccgatcac
     4621 ggtcgttccc ggaatcgaac tgacccatat ccctcccgat gtgatcgggg agctggccgt
     4681 cgagtgccgg cggctcgggg cgaaaatcgt cgtcgtgcac ggtgagacga tcgtggagcc
     4741 cgtagcaccg ggtacaaacc tcgccgctct gaattcgtcc gtcgacatcc tggcccaccc
     4801 gggactgata acagaggagg aggcgaaaat agcggcaaag aacggcgtct atctcgaaat
     4861 ttctacaagg aaagggcaca gcttgaccaa cgggcacgtc ctgaagacgg cccgtctcac
     4921 cggcgccgag ctggtgctga acaccgacag ccatgcgcct caggacatcg tcggcatctc
     4981 gatggggcgg gtgattgccg cgggggccgg gatgacggac gaggaaatcg agcggatgtt
     5041 caagaattcc gccgcccttt tgcaaaagtg cgtgtgatga agtccgtttt tttatccgac
     5101 gtccacttga ggacggaacg ggaccggggg tatcggcgga tactgaactt cctcgacgga
     5161 ataagagatg aggtggaacg gatttttatc gtgggagact ttttcgattt ctggttttgt
     5221 cgaaacggct ccatctatcc gcccttccga gaaccggtcg ataagatcct ggagctggtg
     5281 gaaagaggcg taagaatcag catcttcgag ggtaaccacg acttcgagct tgcggcgtat
     5341 ttcggcggca agggcatcga agtgtacccg gaatgggccg acttcacgtt ggaagggcag
     5401 cgttttttgg tctcccatgg agacacggtg gaccggtcca actatcggta cctcgctttg
     5461 cgtaagatcc tccggacggg agccttttac cgcctccagg aaatgatgcc cagaccgctc
     5521 ctgtggcaga tggcctcagc cagctccatc gtcagtaagg gtttgaccgt tgagtcgcag
     5581 aaggtcctgg cggagaagat gggtgccttt gcgagagaaa aatttgagga aggggtcgac
     5641 gcggtagtcc tcggtcactg ccacataccg ctcctgagac agtacgaagt gaagggaagg
     5701 ctgaagacct tcgttaccct gggtgactgg gtcgagcact tttcctacct tgttttcgag
     5761 gaggggagat tcgagctttc tttctccgag tcttgattgt tctcttgcat atacaatgag
     5821 ttaccgcgat acaggtcgta ccggccgttg tcctgaaaaa aattggacag gtatctgctg
     5881 atgtgttaga tagcccgata aatctcaagg agcggggtta tgaatttcgg ggggatcgag
     5941 gcccgttacg ccgctttcga atctgctgcc ttcgtcgtgg tgcccgtccc gtacgacctg
     6001 acaacgactt atcagagcgg gtcgaggcgg ggaccccagg ccattcttga agcgtcgggc
     6061 aacatggagt tgtacgacga agaattgggc ggcgagactt atcgggcggg tatccatacc
     6121 c
//
LOCUS       DAOTMX010000066         6119 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_11022_length_6119_cov_3.689149, whole genome shotgun sequence.
ACCESSION   DAOTMX010000066 DAOTMX010000000
VERSION     DAOTMX010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 6119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6119
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11022_length_6119_cov_3.689149"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /locus_tag="P5573_02905"
     CDS             complement(<1..935)
                     /locus_tag="P5573_02905"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRT27048.1"
                     /translation="MKQFVFYNPTRIVFGEGKTSDIGRYLKGQKCLFLYGSSSIKQNG
                     IYDKVVDSLKNHGITFVEKSGVKPNPVLSFVHEAIDLARKEQVDCILAVGGGSVIDTA
                     KAVAAGFYYDGDVWDFFVDKARITKALPIYVVLTLSATASEMNSGAVITNETTKQKFN
                     IQGESLFPKTSILDPTNTYTVPKRQVANGSVDAIVHLLEGYFTGKYPNTPIQDGFVET
                     LVKAMIASTKKILENPTDYDARANFMWSATLALNGLTTAGIGEYGFPNHMMGHSLSAL
                     YNVDHGASLSIVFPAWLKYNESTLSDRLAHFGKEV"
     gene            complement(973..2019)
                     /locus_tag="P5573_02910"
     CDS             complement(973..2019)
                     /locus_tag="P5573_02910"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRT27049.1"
                     /translation="MNGSAMHYRQLGKSGLLVSELCLGTMSFGGQGYWEKIGGLDQAS
                     AQRLVDIAFDAGVNFFDTADVYSYGQSEEILGKAIKGKRDKVVIATKVRGRMSPEIND
                     VGLSRRHIIQGCENSLKRLGTDYIDLYIVHSFDFLTPLEETLSALNDLVHQGKVRYLG
                     CSNYFAWQLMKALAVSEKHNWEKFVSLQAYYSLIARDVEIELVPLCQDQGLGVTPWSP
                     LTAGFLTGKYPRGGKGPRDARRSKEEQNFLQFDEDRAYAILGEVERIAHARGVSPAQV
                     SLNYLLRKPAVSSVIIGATKPEQLLDNLKTVEWELTAEEIAALDAVSEPPRIYPRWML
                     EFTRRDRDNPQMMI"
     gene            complement(2140..2886)
                     /locus_tag="P5573_02915"
     CDS             complement(2140..2886)
                     /locus_tag="P5573_02915"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRT27050.1"
                     /translation="MRLKGKTALITGASKGIGRAVAIGFAKEGANLFLTAKDDRQGLE
                     ATLEEVRSLGVKGEGGLYDATNLNDVEKLMDGVERTYGTLDILVNNAGIIKPTPFWEL
                     NPEQFEKTVRTHLFGTYYHTWTATKRFMMPKSSGKVINLAAPAAFRGFFGVVDYASAK
                     GGIVAFTKNVAHELAPYNIQVNAIVPVAETRMTDALSDFYADRFGAAEGKRLQNLPKA
                     ERLVGTFVYFASPDSDYVTGQVLAADGGMY"
     gene            complement(2911..3699)
                     /locus_tag="P5573_02920"
     CDS             complement(2911..3699)
                     /locus_tag="P5573_02920"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRT27051.1"
                     /translation="MKMENRLQGKRILITGASAGIGEACAARFAAAGAELVLCARRID
                     RLRELQAELVEQYHGQVDAFPVDVRDREAVQSFVSEIAERKLIPDILINNAGLAAGLN
                     RFQNGDWNDWDRMIDTNIKGLLNVSRLVIPLMIAAGGGHIVNVGSLAGYQVYPKGNVY
                     NATKFAVRALSEGMNIDLAGTGIRVSMVSPGAVRTEFSEVRFHGDREAAAKVYEGYTP
                     LSAADAAEAVFFIINAPPHVNIQNILITPTAQRNAYCVERDGKT"
     gene            complement(3709..4041)
                     /locus_tag="P5573_02925"
     CDS             complement(3709..4041)
                     /locus_tag="P5573_02925"
                     /inference="COORDINATES: protein motif:HMM:NF014939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HRT27052.1"
                     /translation="MDAEQLYETLKKTQEPKGFFFNRDEDWVLGILNGLLINKERYGY
                     MSCPCRLASGNRAQDQDIVCPCAYREADVKEYGSCYCNLYVSHAWNEGLIDHVYVPER
                     RPPEKMGI"
     gene            complement(4041..4292)
                     /locus_tag="P5573_02930"
     CDS             complement(4041..4292)
                     /locus_tag="P5573_02930"
                     /inference="COORDINATES: protein motif:HMM:NF012675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HRT27053.1"
                     /translation="MSAPVKLFSLSTCSHCKATKKFLTDNGVPYDYTDVDLLSGKERQ
                     QILDEVVKYNPSRTFPTILIGDKVVIGFDEKAIREALGL"
     gene            4611..>6119
                     /locus_tag="P5573_02935"
     CDS             4611..>6119
                     /locus_tag="P5573_02935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRT27054.1"
                     /translation="MKGKTPSKPEQYGRPVQRPAIVHSDSDDKQKNILNCLSDGAYCL
                     DREGRFTFVNEVLVEWSGVPAEEFYRLHYLDMLLPEDHEKARADFKRVMAGEEGIPYE
                     AVYVDAKGEKRAVELHANPVHREGMVVGLLGIARDVTARKQAEDALRESEERYRTILD
                     NILEGYHEVDLAGRFQSCNESFLKMLGYSREELIGTSYKKYTVDEENANKIFQAYNRL
                     YRTGEPVEGLECCMRRKDGERRILEASALLIRKGNADPTGFRAIVRDITERKRMEEAL
                     RESEERYRTILDEMEEGYHEVDLAGNFTFFNEAFLRIFGYSGEEMLGTNYSVYAADEE
                     IAKRVYRAYNEMYKTGAPLKRYEWDVIRKDGARRTHEFCASLLRDSDGRPTGFRGIFR
                     DVTEHRRAVKALKENEEKYRSILESMNDGYFEADLWGNMTFFNPILPKFLGYTREEMA
                     GMNHRVYLDAENAEVVERNCLRLLRGDIPSITVSHEVVKKDGVRAHVETLFSL"
BASE COUNT         1355 a         1621 c         1696 g         1447 t
ORIGIN      
        1 aatacctcct tgccgaaatg tgccaggcgg tcggatagag ttgattcatt gtacttgagc
       61 cacgccggga acactatcga caggctcgcc ccgtggtcga cgttgtacaa tgccgacagg
      121 gaatggccca tcatgtggtt ggggaatcca tactcgccga tgccggcggt ggtcagtccg
      181 ttgagggcaa gcgtcgccga ccacataaaa ttagcccttg catcataatc cgtggggttt
      241 tccaatattt tcttggtgga agcgatcatg gctttcacca gcgtttcgac aaacccgtcc
      301 tgtatggggg tgttcgggta ttttcccgtg aagtagccct ccaacagatg cacgatggca
      361 tccacgctgc cgtttgccac ctgcctcttc ggtacggtgt aggtgtttgt cgggtctaat
      421 atggatgtct tagggaacag gctctcgcct tggatgttga acttttgttt cgtggtctcg
      481 tttgttatca ccgcccccga attcatttcc gaggccgttg ccgacagcgt aagtaccaca
      541 taaatcggaa gggcttttgt gattctggct ttatctacga agaaatccca cacatcgccg
      601 tcgtagtaaa accccgcggc gaccgccttt gccgtatcga tcacgcttcc gcctcccacg
      661 gccaatatgc aatcaacttg ctctttcctt gcgaggtcga tcgcttcatg gacaaaactg
      721 agcacggggt tcggtttgac accgcttttt tcgacgaacg taatgccgtg gttcttcaaa
      781 gaatctacta ccttatcgta aataccgttt tgctttatac tggaggaccc atacaggaag
      841 aggcatttct ggccttttaa gtacctgccg atgtccgatg tcttaccttc accgaatacg
      901 atccgcgtgg gattgtaaaa tacaaactgt ttcatacagc cggctccttt ctcaaattta
      961 aaaaatcttt tatcatatca tcatctgcgg gttgtcccgg tctcgccgcg tgaattcgag
     1021 catccagcgg gggtagatcc gcggcggctc gctcacggca tcgagtgcgg cgatttcttc
     1081 tgcagtgagt tcccattcca cagtcttcag attgtccaaa agctgctccg gtttggtggc
     1141 cccgatgatt acggaactta ccgccggttt tctcaataga tagttgagcg agacctgggc
     1201 aggactcacg cctcttgcat gggcaatgcg ctcgacttca ccgaggatgg cataggcgcg
     1261 gtcttcgtcg aactggagga agttctgctc ttccttgcta cgcctggcgt cccttggtcc
     1321 cttgccgccc cgcggatact tgccggtgag gaacccggct gtcagaggcg accacggcgt
     1381 gacacccaac ccttgatcct ggcacagcgg cacaagctcg atttccacat cccgtgctat
     1441 cagcgagtag tacgcctgga gcgacacgaa cttctcccag ttgtgctttt ccgagacggc
     1501 aagcgccttc atcaactgcc aggcaaagta gttcgaacag ccgaggtatc gcaccttgcc
     1561 ttgatgaacc agatcattca gggccgaaag tgtctcttcg agcggagtca agaaatcaaa
     1621 actgtgcacg atgtaaaggt cgatgtaatc ggtacccagt ctcttcaagc tgttttcgca
     1681 accttggatg atgtggcgcc ggctgagccc cacgtcgttg atctccgggc tcatgcggcc
     1741 gcggaccttg gtggcgatca ccaccttgtc gcgtttgccc tttatagctt ttcctaagat
     1801 ttcctccgac tgtccgtagg agtaaacatc ggccgtgtcg aagaagttga cgccggcatc
     1861 gaaggcgatg tcgacgagcc gctgggcaga agcctggtcg agcccgccga tcttctccca
     1921 ataaccctgt ccgccgaagc tcatggttcc cagacacagc tcagatacca ggagaccgga
     1981 cttgcccaac tgacggtagt gcatcgcaga accattcatg agctgatcct ccttttctta
     2041 agattatctg atggtaccat ctttttcgtt ccaatcgagt gcgacgatcc tttgtccgtt
     2101 cactaccgct gcctcatctg aagcacggta gacagttgat caatacatac cgccgtcagc
     2161 ggcaagcacc tgaccggtga cgtaatcgga atcagggctt gcaaaataga caaaggtgcc
     2221 gactagccgc tcggccttcg gcagattctg aagcctcttt ccttcggcag cgccgaaacg
     2281 gtcggcgtaa aagtccgaaa gggcgtcggt catgcgtgtt tcggcgacag ggacaatggc
     2341 attgacctgg atgttatagg gtgcgagttc gtgggctaca ttcttcgtga atgcgacgat
     2401 accacccttg gctgaggcgt aatcgaccac cccaaagaac cctctgaaag ccgcaggagc
     2461 ggcgagattt atgacctttc cgcttgattt cggcatcata aaacgcttgg tagccgtcca
     2521 ggtgtggtag taggtgccga acagatgggt ccggacagtc ttctcaaact gttccggatt
     2581 aagttcccag aaaggtgtgg gcttgatgat gccggcattg ttgacaagga tgtcaagcgt
     2641 cccgtaggtc cgctcgacgc catccatcaa cttttcgacg tcgtttaagt tcgtggcatc
     2701 gtaaagtccg ccttctccct tcacccccag cgaacgaacc tcctcgagcg tcgcctccaa
     2761 gccttggcga tcatctttag cagtcaaaaa gaggttcgcc ccttctttgg caaacccgat
     2821 ggctaccgca cggccgatgc ctttacttgc gcctgttatc aaagccgttt tccctttgag
     2881 acgcataacc cctcccgttt ttcccgcaaa tcaggtcttg ccgtcgcgct ctacgcagta
     2941 agcgttccgc tgggccgtgg gggttatgag gatgttctgg atgttcacgt gcggcggggc
     3001 attgatgatg aagaaaacgg cttcagccgc gtctgcggcg gaaaggggag tgtaaccctc
     3061 gtagaccttg gcggcggctt cccggtcgcc gtggaagcgc acttcggaaa attccgtgcg
     3121 gacggcgccg ggggagacca tcgagacccg gatacctgtc cccgcgaggt cgatgttcat
     3181 gccctcggag agggctcgaa cggcgaattt ggtcgcgttg taaacgttgc ccttaggata
     3241 gacctgataa ccggcgagac tgcctacgtt gacgatgtgc ccccctccgg cggcgatcat
     3301 cagggggata acgagtcggg aaacgttgag gagccccttg atgttcgtat cgatcatccg
     3361 gtcccaatcg ttccagtcgc cgttttgaaa acgattcaac cccgcggcaa gccccgcgtt
     3421 gttgatgagg atgtcgggta tcagctttct ttcagctatt tcggagacaa agctttgcac
     3481 cgcctcacgg tcgcggacgt cgacggggaa ggcatctacc tgtccgtggt actgctcgac
     3541 cagctcggcc tgcaattcac ggaggcggtc tatgcgccgg gcgcacagta cgagttccgc
     3601 cccggcggcg gcgaaccgtg ccgcgcaggc ctcgccgatg ccggcgctgg ccccggttat
     3661 cagtatcctt ttaccctgga ggcgattttc catcttcatt cctaatgctc agatgcccat
     3721 cttttcgggg ggacgacgtt ccgggacgta gacgtgatcg atgaggccct cgttccacgc
     3781 atgggatacg tagagattac agtagcaact cccgtattcc ttgacgtccg cttcccggta
     3841 tgcgcagggg cagacgatgt cttggtcctg cgcccggttt cccgacgcca gccggcaggg
     3901 acaggacatg tagccgtacc gttccttgtt aatgaggaga ccgttcagga ttcccaacac
     3961 ccagtcctcg tcgcggttga agaagaaacc ctttggttcc tgggtctttt tgagcgtctc
     4021 gtaaagttgc tcggcgtcca tcacagtcct aaagcctccc ggatagcctt ttcatcgaag
     4081 ccgatgacga ccttatcccc gattaggatc gtagggaagg tgcgactggg attgtacttg
     4141 acgacctcgt cgaggatttg ttgcctctcc ttgccgctca gaaggtccac gtcagtatag
     4201 tcgtaaggaa cgccgttgtc ggtgagaaat ttcttcgtcg ccttgcagtg gctgcacgta
     4261 ctcagggaaa acaactttac cggtgcagac atgatttgtt acctccgaag cggatgatta
     4321 tggcgcaatc gggtaaaatg tgcctgtttt tactatctat gattttgaat ttatagtaca
     4381 atttccaaag cttttcaacg gaaatcgcct cggttttcat cgctgctgct gtcaacacct
     4441 ctgtatagaa ctttcccatt cggcaagttt gagcgaccgt tccagcctgt gggttcgtga
     4501 ctcagacacc cgggcgtcga gcaatgctgg aacaataatt gcaagtattt taaacttgga
     4561 aaagcttagt agtcggcctt gccgcacaat tcaaaatcat tgaggatgcc atgaaaggga
     4621 aaacaccgtc caagcccgag cagtacggtc gacctgtcca acggccggcc atcgtccatt
     4681 ccgacagcga tgacaaacaa aagaacatct tgaattgcct cagcgacgga gcgtattgtc
     4741 tggaccgcga aggacgtttt acctttgtta acgaggtact cgtggagtgg tctggagtcc
     4801 ccgcggagga attttatcgg ctccattatc tcgacatgct tcttcctgaa gaccacgaaa
     4861 aagccagggc agacttcaag cgggtgatgg caggggaaga ggggattcct tacgaagcgg
     4921 tttatgtgga tgcaaaaggt gagaagaggg ccgtggagtt acacgcaaac cccgttcata
     4981 gggaaggaat ggtcgtcggc ctattgggca tcgcccgtga cgtcaccgcg cgcaagcagg
     5041 cggaggatgc attgcgggaa agcgaagagc gataccggac catactcgat aatatactgg
     5101 agggctatca cgaggtggac cttgcaggac gtttccaatc gtgcaacgaa tcgttcctaa
     5161 agatgttggg gtacagtcgc gaagaactga tcggtacgag ctacaagaaa tataccgtcg
     5221 atgaagagaa cgcgaacaag atttttcagg cctacaaccg gctgtaccgg acgggcgagc
     5281 ccgtcgaggg gctcgagtgc tgcatgcgga gaaaggacgg ggaaagaagg attttggagg
     5341 cttctgcttt attgatcaga aaaggcaatg ccgaccctac aggtttcagg gcgatcgtcc
     5401 gggatattac cgagcgcaag cggatggagg aagccctccg ggagagcgag gagaggtacc
     5461 gtaccatcct cgatgaaatg gaagaaggtt accatgaagt ggaccttgcc ggtaatttta
     5521 cttttttcaa cgaggcattt ctgagaatct ttggttacag cggggaggaa atgttgggca
     5581 cgaattacag tgtttacgca gcggatgagg agattgcaaa aagagtctac cgtgcctaca
     5641 acgagatgta caagacgggg gctcccctca aaagatatga atgggatgtc atcaggaaag
     5701 acggggcaag acggacgcat gaattttgcg cttcgttgtt gagagattcg gatggtcgtc
     5761 ccaccggctt ccggggtatc ttccgcgacg tcaccgagca cagaagggca gtgaaggcgc
     5821 taaaggagaa cgaggagaaa taccggagca ttcttgagag catgaacgat ggctattttg
     5881 aggccgactt gtggggcaac atgaccttct tcaaccccat actgcccaag ttcctgggat
     5941 atacccggga agagatggcg gggatgaatc accgggtgta cttggacgcg gaaaatgcag
     6001 aggtcgtaga aagaaactgc ctgcgcctcc tccgggggga tataccttct ataaccgtca
     6061 gtcacgaggt cgtgaaaaag gatggagtga gggcgcatgt tgagacgctg ttttctctg
//
LOCUS       DAOTMX010000067         6105 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_11051_length_6105_cov_3.307107, whole genome shotgun sequence.
ACCESSION   DAOTMX010000067 DAOTMX010000000
VERSION     DAOTMX010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 6105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6105
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11051_length_6105_cov_3.307107"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /gene="ftcD"
                     /locus_tag="P5573_02940"
     CDS             complement(<1..696)
                     /gene="ftcD"
                     /locus_tag="P5573_02940"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HRT27055.1"
                     /translation="MKLLECIPNVSEGKDRGKIDAVAAVISAAEGVKLLDVCRDPDHH
                     RTVFTFIGPSGKLEEAALAALGRALELIDMREHRGVHPRIGAVDVVPFVPLAGSDMED
                     AVKAAHSFGRRFAETYRIPVYFYGEAALRPERRELPSVRRGGYEALGAKLKDPHWRPD
                     AGPAEFNPRSGAAAVGARAPLIAFNVNLRSGDLDAAKKIARAVRHSSGGLSHVRAIGV
                     LLKSRGIAQVSMNL"
     gene            complement(702..1343)
                     /locus_tag="P5573_02945"
     CDS             complement(702..1343)
                     /locus_tag="P5573_02945"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRT27056.1"
                     /translation="MGKIFATGDIHGRLDKLEDLMDVVEADHNEDTLVFLGDYIDRGP
                     DSAGVVEFILDLRETYRNVVCLRGNHEEMFLDYVLKKKSRDLYFYNGGRYTLRSYGLD
                     PDTPPSESDFPKRHMDFFKSLPYYWETEDYIFVHAGLRPGRPLERQDKDDMVWIRGEF
                     IFARCNFGKTVIFGHTPFSVPFIEDCRIGIDTGAAYSGPLTCLELPAKKFHQV"
     gene            complement(1351..2283)
                     /gene="ftsY"
                     /locus_tag="P5573_02950"
     CDS             complement(1351..2283)
                     /gene="ftsY"
                     /locus_tag="P5573_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HRT27057.1"
                     /translation="MFKKKEGGGFFDRLKTGLEKTRKTLASGIEAVIFGERVIDSNLY
                     EELEAVLVSADVGAVFASELIDEIKEEVKRKELNNPAVLKNILMERMADILKAVDKPL
                     ELPKNGPYAVMVVGVNGTGKTTTIGKLAHYFREEDRSVMLVAADTFRAAAIEQLEVWS
                     KRSGASFVKQKMGSDPSSVVFDAVKAALSRKTDVMLIDTAGRLHTKSNLMEELKKMKR
                     VMGRELPGSPHEVLLVLDATTGQNAVVQAKMFNEEVGVTGIVLTKLDGTSKGGIVVRI
                     AHELKIPVRFIGIGEGIDDLRPFDGEEFVKAIFE"
     gene            complement(2312..5866)
                     /gene="smc"
                     /locus_tag="P5573_02955"
     CDS             complement(2312..5866)
                     /gene="smc"
                     /locus_tag="P5573_02955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HRT27058.1"
                     /translation="MKLKKLEILGFKSFRDRTVLDFSSGISAIVGPNGCGKSNIVDAI
                     RWVMGEQSARLLRGKKMEDVIFNGSDEAPPVNMAEVSIVLADDGRGFPGDYSECTEVM
                     ITRRVFREGESEYFINKVPCRLLDVREFFMDTGVGARTYSLVEQNSISDLVEAKPEDR
                     RLFIEEAAGIRKYRSRKEAATRKMEATRENLNRLTDIIREVKSQLNAVSRQAKRAERY
                     KALKQEIKEADLTLSLHHRSLLTEKIEEISQTVETLNAQEVAIKTKLRQIEASTESLR
                     ADLAVSEKTLQELHEKTYVTKNSIGYKEQNLEFLRVKLGDLDKRKAKDLADTESLKER
                     LNKFEREASSLESQLVELSEKAAKVREGIVDKQEKAASMRKADRALQEEIEGKKNVHV
                     EILTENARLKNMVASYAKGVEDLKRRAEREEKDIEENLRRLGDIESSLSELRTKVKEK
                     ETYSEGLKDKEEALVSTLNEQRKALRESEENIVYLKEEKGKKAARLASLKEFHEGYLW
                     CSEATRSIMKDKDQILAFGAVHGLVADHVEVPQEYEVAVEAVLGEKLQYVVVRSQEDG
                     IQAIDYLKAHSTGRGSFIPLEVRGHEVTETPGYLAGAVKLIDRVKVKEDFQKIADYLL
                     GDVLLIPNLEMGLSLWRRNGFSGTFVTPEGDIITPHGVLTGGNGVGTESSLLRDKREI
                     SELEQEVERFEALLGEQNTRRDGLKESIRQGEEELEGIKSEQRSLELSLHNSLKDIEG
                     LEKEAGWVEKRLNVLRFNRENLVAEGAQAAEKISSLEIKIAANEEKCREADEAITALK
                     ERGEQSRKALENLERELTERMVLLKSLEEKEESCRSNLDRLHMEADGLEDEIAAKAAD
                     IEDCDREFLETTRKVEEEREALKSLYEAGRKMEQELAEQTASHRAKEDALRVGEAENK
                     ETRRELEKVVAQAGELQMELQGLRFQAEALKKNALEKYSVDLDGMAAGFQRPDDEQIA
                     ELTEKIVANTKAIEEFGEVNLLALSEHNVLKERYDFLTAQVADLNRSLESLQQTISRI
                     NRVSRQRFSETFEAVNARFKEVFPKLFPGGRGELRLTDESDLLETGIDIDIRTPGKKT
                     QNVMLLSGGEKSLAAVALIFGIILYRPTPFLILDEVDAALDDSNVSLFNRLLSEIAGD
                     SQIIIITHNKKTMEVANSLYGVTMEKSGISTLVSVNMN"
BASE COUNT         1042 a         1816 c         1704 g         1543 t
ORIGIN      
        1 caggttcatc gacacctggg cgatacccct gctcttgagc aggacgccta tggccctgac
       61 gtgactgagg ccgccgctcg aatggcggac cgcgcgggcg atcttttttg ccgcgtcgag
      121 gtcgccgctc ctgaggttca cgttgaaggc gatcagaggc gccctcgcgc cgaccgccgc
      181 ggcgccggag cgggggttga attcggccgg tccggcgtcg ggcctccagt gggggtcttt
      241 cagttttgct ccgagcgcct cgtagccgcc acgacgcacc gaaggcagct cacggcgctc
      301 cggcctgagg gccgcttctc cgtagaaata caccgggatg cggtacgtct cggcgaaacg
      361 gcgaccgaag gaatgggcag ccttcacggc gtcctccatg tccgaacccg cgagggggac
      421 gaaggggacg acgtccacgg cgccgatccg gggatggacg ccccggtgtt cccgcatgtc
      481 gatcagctcg agcgccctgc cgagcgccgc cagggctgcc tcttccagct tcccggacgg
      541 gccgatgaaa gtgaagaccg tccggtgatg gtcggggtcc cggcagacgt cgaggagctt
      601 gacgccttcg gcggcggaga tcaccgcggc gacggcgtct atcttgcctc tatcctttcc
      661 ttcgcttacg ttggggatgc actcgaggag cttcatcgtg ttcacacctg gtggaacttt
      721 tttgccggca gctccaggca ggtgaggggg ccgctgtatg ccgccccggt gtctatgccg
      781 atacggcagt cttcgatgaa gggcactgaa aagggggtgt ggccgaatat caccgtcttc
      841 ccgaagttac agcgcgcgaa aatgaactcg cctcggatcc agaccatgtc gtctttgtcc
      901 tgccgttcga gcggtcttcc gggccggagc cccgcgtgga cgaagatgta gtcttccgtc
      961 tcccagtagt agggcagaga cttgaagaaa tccatatgcc gtttggggaa gtccgattcg
     1021 ctcggcggtg tgtctgggtc gaggccgtat gacctcaggg tgtatctccc gccgttatag
     1081 aaatagaggt cccggctttt tttcttgagg acgtagtcga ggaacatctc ctcgtggttg
     1141 ccgcgcaggc agacgacgtt ccggtaagtc tcccggaggt ccaggatgaa ttccacgaca
     1201 cccgcggaat cggggccccg gtcgatgtag tcccccagaa agaccagagt atcctcgttg
     1261 tggtcagctt ctacgacatc catgaggtcc tccaacttgt cgaggcggcc gtggatatcg
     1321 cctgtggcga atatcttccc catggagcta tcattcgaag attgccttca cgaactcctc
     1381 gccgtcgaag ggccgcaggt cgtcgatgcc ttcgcctatc ccgatgaagc gaaccgggat
     1441 tttcagttca tgggcgatcc tcacaacgat gccccctttt gacgtcccgt cgagcttggt
     1501 gaggacgatg cccgtcaccc cgacctcttc gttgaacatc ttggcctgca cgacggcgtt
     1561 ctgccccgtc gtcgcgtcga ggacgagaag gacctcatgg ggagaaccgg gcagttcgcg
     1621 ccccatgacc ctcttcatct tcttcagttc ttccatcagg ttggacttgg tgtggagcct
     1681 ccccgccgta tcgatgagca tgacgtccgt cttgcgactt agggcggcct tgacggcgtc
     1741 gaaaacgacc gaagaagggt cggaccccat tttctgcttg acgaacgacg ccccggacct
     1801 cttcgaccag acctctaact gctcgatcgc ggcggcccgg aaggtgtcgg cggcgacgag
     1861 catgaccgag cggtcttcct ccctgaaata gtgggcaagc ttaccgatgg tcgtcgtctt
     1921 gccggttccg ttgaccccga cgaccattac cgcataaggg ccgtttttcg ggagttcgag
     1981 gggtttgtcg accgccttca ggatgtcggc catccgctcc atcaggatgt ttttcaggac
     2041 ggcggggttg ttgagttcct tcctcttgac ctcttctttt atctcatcga tgagctcgct
     2101 cgcgaagacc gccccgacat cggcgctgac caggacggct tcgagctctt catagagatt
     2161 gctgtcgatg accctttcgc cgaagatgac ggcctcgata cccgaagcga gggtctttct
     2221 cgtcttctcc agccccgttt tcaggcggtc aaaaaaaccg ccgccttctt ttttcttgaa
     2281 cacaggaatc cttttttcgc tccaatccgg tttaattcat gttgacggag acaagcgtgg
     2341 atatgccact cttctccatc gtgacgccgt aaaggctgtt cgcgacttcc atggtcttct
     2401 tgttgtgggt gatgatgatt atctgagagt ctccggcgat ctccgagaga agcctgttga
     2461 agagagagac gttcgagtcg tcgagggccg cgtccacctc gtcgaggatc aggaaggggg
     2521 tcggcctgta caggatgatg ccgaagatga gggctaccgc agcgagggac ttttcgcccc
     2581 ccgatagcag catgacgttc tgcgtctttt tccccggggt gcggatgtcg atatctatgc
     2641 ccgtctcgag caggtccgat tcatcggtca accttaactc gccccgtcct ccggggaaga
     2701 gtttagggaa tacctctttg aagcgggcat tgaccgcctc gaaggtctcg gagaaccgct
     2761 gtcgcgacac ccggtttatc cgagagatcg tctgctggag ggattcgagg gaacgattca
     2821 ggtcggcgac ctgggccgtg agaaaatcgt accgctcctt gaggacgttg tgttcgctca
     2881 atgccaggag gttgacctcc ccgaactcct ctatggcctt tgtattggcg actattttct
     2941 ccgtcagttc tgcgatctgc tcgtcgtcgg gcctctggaa cccggcggcc atcccatcaa
     3001 ggtcgacgct gtacttttcc aaggcgttct ttttcaatgc ctccgcctgg aagcgaagac
     3061 cttgcaactc catctggagt tcccccgctt gggcaaccac tttttcgagc tcccgccttg
     3121 tctccttgtt ttccgcctcc ccgactctca aggcgtcctc cttcgcccgg tgggaggccg
     3181 tctgttcggc cagttcctgc tccatctttc ggcccgcctc gtaaagggat ttcagggcct
     3241 cccgctcctc ttcgaccttg cgcgttgtct ccaaaaactc ccggtcgcag tcctcgatgt
     3301 cggcggcttt cgcggcgatt tcatcctcca ggccgtcggc ttccatgtgc aggcgatcga
     3361 ggtttgaacg gcaggactcc tccttttctt cgagtgactt gaggaggacc atcctctcgg
     3421 tcaattcacg ttccaggttt tcgagggcct ttctcgattg ttcacccctt tccttcaagg
     3481 cggtgatggc ctcgtccgcc tcccggcatt tctcttcgtt cgccgcgatc ttaatttcca
     3541 gggagctgat cttttccgca gcctgagctc cttcggcaac gaggttttcg cggttgaagc
     3601 gaaggacgtt tagcctcttt tctacccacc cggcttcctt ttcgaggccc tctatatcct
     3661 tgaggctgtt gtggagtgag agttcaaggc tgcgttgctc cgacttgatg ccctccaatt
     3721 cttcttctcc ttgccggata ctttccttca gaccgtccct gcgggtgttt tgttcgccta
     3781 aaagggcctc aaagcgctcg acctcctgtt ccaattcgga gatttccctt ttatctcgca
     3841 ggaggctgct ctcggtgccg acgccattac cgcccgtcag gacaccgtga ggggtgatga
     3901 tgtcgccttc cggcgtgacg aatgtcccgc tgaagccgtt cctccgccag agtgaaaggc
     3961 ccatctccag gttcgggatg agaaggacgt cgcccaggag ataatcggcg attttctgga
     4021 aatcttcttt gaccttcacc cggtcgatga gcttcaccgc gccagcgagg tatccggggg
     4081 tctctgtcac ctcgtggccc cgcacctcga gggggatgaa gctgcccctt cccgtcgagt
     4141 gggccttcag gtaatcgatg gcctggatgc catcttcctg gctcctgacg acgacatact
     4201 gaagcttttc gcccagcacg gcctcaacgg cgacttcata ttcctggggg acctcgacgt
     4261 ggtcggcaac taggccgtgg acggcgccga aggcgaggat ctggtccttg tctttcatga
     4321 tcgaccgggt ggcctcgctg caccataggt acccctcgtg aaattctttg agcgacgcaa
     4381 ggcgggccgc cttctttccc ttctcctctt tgaggtagac gatgttttct tcgctctccc
     4441 tgagggcctt cctctgctcg ttcagcgtcg aaacgagggc ttcttcttta tctttcaggc
     4501 cttcggaata agtctccttc tccttcacct tcgtgcgcag ctcggagaga gatgattcta
     4561 tgtcgccgag ccgcctcaag ttttcctcga tatccttttc ttcgcgctcc gcccgcctct
     4621 tgaggtcttc gacacccttt gcgtaggagg caaccatgtt ctttaggcgc gcgttttcag
     4681 tcaggatttc tacgtggacg tttttcttcc cctcgatttc ctcctgtaag gcacggtcag
     4741 cctttctcat ggaggccgcc ttttcctgct tgtcgacgat tccctccctg actttcgctg
     4801 ccttttcaga caattccacg agttgggact cgagggatga ggcctccctt tcaaatttgt
     4861 tcagtcgttc cttgagggat tcggtgtcgg ccaggtcctt cgcctttcgc ttgtcgaggt
     4921 cgccgagctt tactctaaga aattcgaggt tctgctcctt gtagccgata ctgtttttcg
     4981 tcacgtaggt cttttcatgc aattcctgca aggtcttttc gctgaccgcc agatcggccc
     5041 gcagcgactc ggtcgaggcc tctatttgac gtagttttgt cttgattgcg acttcttggg
     5101 cgttcaaggt ttcgacggtc tgactgattt cttctatctt ttcggtgaga agggaacgat
     5161 gatggaggct gagggtcagg tcggcttctt tgatctcttg tttcagggcc ttgtaacgct
     5221 cggctcgctt cgcctgacgg gaaacggcgt tcaactgaga ctttacttcc cggatgatat
     5281 cggtcaagcg attcaggttc tcgcgggtgg cctccatctt ccgcgttgcc gcctctttcc
     5341 ggcttctgta tttccgaatg ccagcggcct cctcgatgaa gaggcgcctg tcttcaggct
     5401 ttgcttccac gaggtcggag atactgttct gctcgacgag agaataggtg cgggcgccga
     5461 cgccggtatc cataaagaat tcacggacat cgaggaggcg gcagggaact ttgttgataa
     5521 aatactcgct ttccccctct cggaagacgc gccgggtgat catcacctcg gtgcattcgc
     5581 tgtagtcgcc ggggaacccg cggccgtcgt cggcgagaac gatagacacc tcggccatat
     5641 tcacgggggg tgcctcgtcg ctcccgttga agatgacgtc ctccatcttc ttcccccgca
     5701 agagcctggc gctctgctcc cccattaccc agcggatagc gtcgacgatg ttgctcttgc
     5761 cgcacccgtt gggaccgacg atggcgctga ttccactgga aaaatccagg accgtcctgt
     5821 cacggaagga cttgaacccc aggatttcca gcttctttag cttcattcgg ggaacctctt
     5881 ttcccttatc gtatcgtaga attttcggcc attgtcaatc atcaagacgg aaaaggtagt
     5941 tttgaacaat tttggttctt actggaatga catgagagaa ccgtttttcg tatggcttgc
     6001 gtcagggatt tagcagaata tagggtcttt gtaaaggaaa aatacaacaa aatgtattcc
     6061 ctgcaaagga gaccacaata tgttgtgtgt taccccgtca gcttt
//
LOCUS       DAOTMX010000068         5954 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_11376_length_5954_cov_4.024241, whole genome shotgun sequence.
ACCESSION   DAOTMX010000068 DAOTMX010000000
VERSION     DAOTMX010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5954
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11376_length_5954_cov_4.024241"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..615
                     /locus_tag="P5573_02960"
     CDS             85..615
                     /locus_tag="P5573_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27059.1"
                     /translation="MTDGVEGKKAFYIKAKEALESAGYQYFDEHEIKGIGRSHMSKPD
                     YIAVKKNVLVIGEIKSPAESPTSPSWRQIQKSDGEEFKRVRLEVANREREGKVSKEVG
                     GHEIVIRGQIPDYVEKLGKTYLLPFSEHSNMKTILGYSFPTEETFNVEHAFKNCKSKI
                     LKVIDIGNGITTYFFK"
     gene            665..1324
                     /locus_tag="P5573_02965"
     CDS             665..1324
                     /locus_tag="P5573_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRT27060.1"
                     /translation="MSSKATGKIDTNWFVDLLMTFAPKSVETDGKTPEQMTKEASWEA
                     FGISSVAALPPGPIGWATILPELAAVLKIQTNLIYRIATFYGKQGKVNHTLILLIFAN
                     EAGLAIGQTLAKRMGTKLVIRSLGSKALRPIAQKVAARIGARITQRAVGRWVPFVLAP
                     VFGAFSKSMTTKIGREADKLLRQDIEFVEMAECPNGHEVPADVKFCPECGALMKQEIL
                     V"
     gene            1670..2212
                     /locus_tag="P5573_02970"
     CDS             1670..2212
                     /locus_tag="P5573_02970"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HRT27061.1"
                     /translation="MERMQIGKAFTLMEPGPVVLVTTNDGKKNNIMTISWTMVVDFTP
                     MFAITTGPWNYSYAALQKYRECVISIPTVDLLDQVVGVGTCSGADTDKFEKFGLTPVE
                     GGHVRAPLIKECLANIECKVIDIVKKHNIVVLEGVAAYFDSSRKEKRTVHAIGDGTFV
                     VDGRKLNRRKMMLSKLPDGV"
     gene            2239..2448
                     /locus_tag="P5573_02975"
     CDS             2239..2448
                     /locus_tag="P5573_02975"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRT27062.1"
                     /translation="MPYDVLHIPLGALKGKLAEIPRDKDILTFCKISLRGYEAQRILN
                     AAGFDRVQFIEGGIVGWPFEVKVLK"
     gene            complement(2531..2606)
                     /locus_tag="P5573_02980"
     tRNA            complement(2531..2606)
                     /locus_tag="P5573_02980"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2570..2572),aa:Glu,seq:ttc)
     gene            complement(2791..4905)
                     /locus_tag="P5573_02985"
     CDS             complement(2791..4905)
                     /locus_tag="P5573_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRT27063.1"
                     /translation="MAHSSNRHERLKKAERLQHPRKSFPEKTHKRPHKPHLKQKGPEA
                     EPVLKGVFSRIGKPEEAPFQPDTFQLEALEAIKRTDCLVTAPTGSGKTWIAEQAIRSV
                     FAAGGRAWYASPLKALTNSKWVEFGLSFNDKNVGILTGDTKENTDAPIIVGTTEILRN
                     QLYDAMYHGEDLNCDLVILDEAHFLGDEDRGVVWEEIMIYLPRRVNLLLLSATIGNSH
                     EIASWLSSIRKKECVVVEETKRPVPLYPLFLHPSGRVMPFLNKKRLYGKVEALLKGED
                     SRNKRGNRFSAFGEIIETLRHFDLLPAIFFLKSRSDCDSALRMCRRRTEEWETDGFGY
                     YLEELLERFPYLKNHKQLPYLSLSRVASHHGGQLPAWKFLVEAMMKKGYLDAIFATTT
                     VAAGVNFPARTIVLFNSDQFNGHEFLPLTATGFHQMTGRSGRRGQDRIGFMMLVPGRF
                     MDMKHVRELLFSPPDRIESRIRSDFSMALNLLLSHTPEDVKEMFERSLAAHQHSGKKK
                     RDTEDTSLLLWKDFQKHLAFLKQEGFVDDNERLTEDGLWASKLRLDKPLLIAECLRKG
                     VFPEDDSALLAGIIAPFVQDGDSEIRLDRRDVPRKLRFLYKDVMSTIAPLAERMKTAG
                     FPVGRLPLWAAVLMYHWARGMEWERIIRLMGIAEGDLTMLVSRTADNLRQIASLKDTY
                     RQMAALALDARERILREPVVFE"
     gene            5131..>5954
                     /locus_tag="P5573_02990"
     CDS             5131..>5954
                     /locus_tag="P5573_02990"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRT27064.1"
                     /translation="MGKRRGIYWGWYVVVGAFLVLSVNYGARYCFGVFVNPLADQFKW
                     SRSVISLGASLNMFVYSLCSIFTGRIADRVAPRWIITVGALISSLGFVLTSFIRTPLE
                     FYVVYGLLIGISSSGMGVVVANTSVGKWFVRKRGLAIGFATMGISFGTIVLAPTAGYI
                     VKNMSWQAGFLFMGVVIFLIGVTLSQLLMRRSSPESYGLLPDGEVARDSVLDVGAETQ
                     VTKMSYRDMLRDGRFWVIGVSFGLALMTVMSAFVHQVAYAQDNGIERVAAASSLGAV"
BASE COUNT         1356 a         1453 c         1727 g         1418 t
ORIGIN      
        1 atcttttctt cgtgcccttc tgaggcccaa gggagcggac ccgccggagg gatggattta
       61 tctcgatgat atgatggagg aaaaatgacg gacggggttg aaggaaagaa agctttttat
      121 attaaagcga aggaagcatt ggagagtgct ggctatcaat actttgacga gcatgaaatc
      181 aagggcatag gacgttctca tatgagtaaa cccgattata ttgctgtcaa gaaaaatgtt
      241 cttgtgattg gtgagataaa atctccggcc gagagtccaa catcgccaag ttggagacag
      301 atccagaaaa gtgatggcga agaattcaaa agagtccggc ttgaagtggc taatagggaa
      361 cgagagggga aagtctcaaa ggaagtcggt ggtcatgaaa tcgtcatacg aggacagata
      421 ccggattatg ttgagaaact cggaaagact tatcttcttc ccttttcaga gcatagcaat
      481 atgaaaacga ttttgggtta ttctttcccg acagaggaaa cattcaacgt agaacatgcc
      541 tttaagaatt gtaaatcaaa gatactgaag gtaatagata ttggtaacgg aataactaca
      601 tattttttta agtaatcgtg aataggtttt tgatttcatt atgttaaaga aaggaggata
      661 agaaatgagt tcaaaagcca caggcaagat tgacacaaat tggttcgtag atttgctaat
      721 gacatttgcg ccaaagtcgg ttgaaactga cgggaagacg ccagagcaga tgacgaaaga
      781 agcatcatgg gaagcattcg gcattagctc tgttgcggcg ctgccacccg gtcctattgg
      841 ttgggcaaca attctgccgg agttggctgc ggttttgaag atccagacaa atctcattta
      901 ccgcatagct acgttttatg ggaaacaagg caaagtcaac cacaccttga tattgcttat
      961 tttcgccaac gaggcaggtc tcgctatcgg tcaaacacta gctaaaagaa tgggaacaaa
     1021 gctagtaatc cgctcccttg gatcaaaggc actccgccca atcgcccaga aagtcgcagc
     1081 aaggattggt gcccgtataa cacaaagagc tgtaggacgg tgggtaccct ttgtccttgc
     1141 tcctgtcttt ggcgcattct caaaaagcat gacaaccaag atcggccgtg aggcggacaa
     1201 gctccttcga caagacatag aattcgtcga gatggcggag tgccccaatg ggcatgaggt
     1261 tccagcggac gtgaagttct gtccagaatg tggtgctttg atgaagcaag aaatcttggt
     1321 gtagggtttt cttggacacc gaattaggat agatcagaga tacaaaaggc gtaaaaatca
     1381 atctcttccc ggatagttgc cgtctctccg gcaatgaatt tagctgcgag gcctttccgt
     1441 agcccttcgt tttttgaggg cctgttctaa gatgatgtcg aaaagagatg gtatccaaaa
     1501 gctatctatt taatgtcgct tggcttttgt gaatgtgggt ggagagacct ctctactctg
     1561 ccgtgggtaa aagtaatgtc cttagagcga ttcggtgaga aaaacagggt cttcgtgaaa
     1621 ttaggaaata taaaaaagcc tcatgaaatc cgccttggag tgcgcacgta tggaaaggat
     1681 gcagatcggt aaagccttca cgcttatgga acccggccct gttgtgcttg ttacgacgaa
     1741 tgacgggaaa aagaacaaca taatgaccat ctcatggacc atggtggtgg actttacgcc
     1801 gatgtttgcc ataaccaccg ggccctggaa ctattcctat gctgcgcttc agaaatacag
     1861 ggaatgcgtg atttcgattc caacggttga cctgcttgat caggttgtcg gcgtgggaac
     1921 gtgttcaggg gccgacacgg ataagtttga gaagttcgga ctgactcctg tggagggagg
     1981 gcatgtcagg gcgccgctga tcaaggaatg tctggcaaat atcgaatgta aggttatcga
     2041 tattgtcaaa aagcacaata tcgttgtcct cgaaggcgtc gctgcttatt tcgatagttc
     2101 acgaaaagag aagcggaccg tacacgccat cggtgatggg accttcgtgg tggatgggcg
     2161 taagttgaac cgaaggaaaa tgatgctgtc gaagcttccg gacggggtgt aaatcgttgg
     2221 tgggccttat attggtatat gccttacgat gtccttcaca tcccgctggg ggcgttgaaa
     2281 ggcaagcttg cagagatacc cagagataag gacatcctca cattttgcaa gatcagcttg
     2341 cgcggctacg aggcgcagag gatcctcaat gccgccgggt tcgacagggt gcaattcatc
     2401 gaaggcggta ttgttggctg gccctttgag gtgaaggtgc tgaaataggt gcggtcaata
     2461 ggagcaggga cgtcgctacc ggcgtccctg cgattcggcg gtagagaggg ttccggcctg
     2521 ctcagagtat tggcgtcccc acggagattc gaactccggc tgccggcgtg aaaggccggt
     2581 gtcctaggcc tctagacgat ggggacgcgc tgatcaatat gatgggtttc cgctgttacg
     2641 gaaacggcaa aattgcgtgc gcggcgggta agagaaaaaa aacaggccga ccgtttgaag
     2701 tgggtgcttt tataattgtt aagctcccgg atgtcaagag aaaagacgca cttactcttc
     2761 gctgaatgcg aacgagcttt aggctgcagt ctactcgaag acgacaggtt cgcggaggat
     2821 cctctcccgc gcatcgaggg cgagggcggc catctgccgg taagtgtctt tcaatgatgc
     2881 gatctgcctc aggttgtccg ccgtccggga aacgagcatc gtgaggtccc cctcggcgat
     2941 ccccatcagc cggatgatcc tttcccattc catcccccgc gcccagtggt acatcaggac
     3001 ggccgcccag agaggaagcc gtcccacggg gaaccccgcc gtcttcatcc tctctgccag
     3061 cggggcgatt gtcgacatca cgtctttgta aaggaagcgg agcttgcgcg ggacgtccct
     3121 ccggtccagt ctgatttctg aatccccgtc ttggacgaaa ggggcgatga ttccggcaag
     3181 aagcgccgaa tcgtcctccg ggaaaacccc cttccgcagg cactccgcga tgaggagcgg
     3241 cttgtcgagg cgtagtttcg aggcccagag cccgtcctcc gtcagcctct cgttgtcgtc
     3301 gacgaacccc tcttgcttca ggaaagccag gtgtttctgg aagtctttcc aaaggagcag
     3361 gctcgtgtcc tcggtgtctc gtttcttctt gccgctatgc tggtgagccg ccagggacct
     3421 ctcgaacatc tctttcacgt cctcgggggt atgggagagg aggagattca gggccatgga
     3481 aaagtcgctc cggatgcggc tctcgatcct gtcgggtggc gaaaatagca gctcccgcac
     3541 gtgcttcatg tccatgaagc ggcccggcac gagcatcatg aaaccgattc tgtcctgtcc
     3601 acgccttccg gaccggcccg tcatctgatg aaaccccgtc gccgtcaggg gcaggaattc
     3661 gtgaccgttg aactggtcgg agttgaagag cacaatcgtc cttgccggga aattcacgcc
     3721 ggcggcgacg gtcgtcgtcg caaagatcgc gtcgaggtaa cccttcttca tcattgcttc
     3781 caccaggaat ttccaggcgg ggagctgacc gccgtgatgg gatgccaccc gtgagaggct
     3841 gaggtaagga agctgcttat ggtttttcaa gtaggggaac cgctcaagga gttcctccag
     3901 gtagtagccg aacccgtctg tttcccattc ttccgtcctc ctgcgacaca tcctgagggc
     3961 gctgtcgcag tcggacctgg acttgaggaa gaagatggcc ggcaggaggt cgaagtgcct
     4021 gagggtctcg atgatctcgc cgaaggcgga gaaccggttg ccgcgtttgt tcctgctgtc
     4081 ttcccctttc aagagggctt cgaccttgcc gtacagccgt ttcttgttca agaagggcat
     4141 gacccggccg ctcgggtgca agaaaagggg atagagcggg acgggtcgct tcgtctcttc
     4201 gacgacgaca cactccttct tcctgataga gctcagccag gatgcgattt cgtgactgtt
     4261 gccgatggtg gccgaaagga ggagcaggtt tacccgccgg ggcaggtaga tcatgatttc
     4321 ttcccagaca acgccccggt cttcgtcacc taggaaatgg gcctcgtcga ggatgacgag
     4381 gtcgcagttc aggtcctcgc cgtggtacat ggcgtcgtag agctggttcc gcagtatctc
     4441 ggtggtcccg acgatgatgg gcgcatccgt gttctccttg gtgtcgcccg tgaggatccc
     4501 cacgttcttg tcgttgaagg agaggccaaa ctccacccac ttggagttgg tcagggcctt
     4561 aagaggcgag gcgtaccagg cccgcccccc cgcagcgaag acggaccgga tagcctgctc
     4621 cgcgatccag gtctttcccg accccgtcgg cgccgtcaca aggcaatccg tccgcttgat
     4681 tgcctccagc gcctcaagct gaaaggtatc cggctggaag ggcgcctctt ccggcttgcc
     4741 gatccgggag aaaacgccct tcagtaccgg ttcggcctcc ggcccttttt gctttaaatg
     4801 gggcttgtgg ggcctcttat gggttttttc ggggaaggat tttctcgggt gttgcagtct
     4861 ttccgctttc tttaaccgtt catgcctatt tgaggaatgg gccatttaaa tcgtgctcag
     4921 atttatcgct ttcgttaatg attatctcga tttattgatg ctgcatcata ccataaacgc
     4981 accgaaagta aaggccgaac caagttatgg tttgacaaag agaggagggc catattaaaa
     5041 accgcaattc cgatggaccc catagccgcc ggtatgaggg tgcaaagggc acggaaataa
     5101 aaagggagaa gaagtcccgc aaggccgtaa atgggaaaga gacgcggtat atactggggt
     5161 tggtacgtag tggttggggc ttttttggtg ctgagtgtca attacggtgc ccggtactgc
     5221 ttcggtgtct tcgtcaaccc cctggcagac cagttcaagt ggtcgaggtc ggtgatctcc
     5281 ctcggtgctt ctctcaacat gttcgtctat tccctttgct ctatctttac cgggaggata
     5341 gccgaccgtg tggcgccgcg gtggatcatc accgtcgggg cgttgatttc ctctctgggc
     5401 tttgtcctta cgagcttcat ccggacgccc cttgagttct acgtcgtgta tggtttactc
     5461 atagggatca gttcgtccgg gatgggggtc gttgtggcca atacgtcggt gggcaagtgg
     5521 ttcgtcagga agagggggct cgctatcggg ttcgctacca tgggcatcag cttcgggacg
     5581 atcgtccttg cacccacggc gggctatatc gtgaaaaaca tgagctggca ggccggcttc
     5641 ttgttcatgg gggttgtgat cttcttgatc ggtgtcaccc tctcccaact cctgatgaga
     5701 cgctcgagcc cggagtctta cgggctcctg cccgacggcg aggtcgcaag agacagtgtc
     5761 ttggatgtgg gggcggaaac acaggtcacg aagatgtcct accgggatat gttaagggac
     5821 ggtagattct gggtcatcgg cgtctccttc ggtcttgccc tgatgaccgt gatgtccgct
     5881 tttgtccacc aggtagccta tgctcaggat aacggtatcg aacgggtggc cgcagcctct
     5941 tcgctcggcg ccgt
//
LOCUS       DAOTMX010000069         5948 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_11387_length_5948_cov_3.261497, whole genome shotgun sequence.
ACCESSION   DAOTMX010000069 DAOTMX010000000
VERSION     DAOTMX010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5948
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11387_length_5948_cov_3.261497"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1521
                     /locus_tag="P5573_02995"
     CDS             <1..1521
                     /locus_tag="P5573_02995"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27065.1"
                     /translation="MTAWNHRGLVESRDSPYGRVTVTKLDGQVSVFENDAIAFETQGT
                     EAETFANLAALQHPGPESVLILGGGVEGTVKEILKQSPKRVDYVELNPVMVEAVLPHL
                     PADIRRSFEQPGVRLIVDDPRKFLRGSGRYDLILIGMPEPSSGQSNRFFTREFFSLCA
                     ERLNPLGVVGLRLRSAENFWTPQLRRRTASIYHALASVFPSVLVLPGATDIITASRSP
                     LPTAPEVMVERLERRKVEARLVSAPYICYLFTNDRFAEVNGTLRASSVQPNTDSRPVG
                     YQYALMTWLAKFFPQLALPDPVEYVEFYVAKGMGYWAGAVVLTIFLLARLRPSWRNAM
                     LVFTAGFLGMVLETVLILHYQVKSGVLYQDIGILLTLFMAGLALGSALFDRGVFRRDR
                     RRRSALWIGAGMMALFGLYCLLIMVQVKADNLAGLTTTAGLLGGLGVLVAGVFGYASL
                     HDRQDQDRVVSPLYAADLIGGCLGSILASLVMIPLLGLDVTLQGMTMLALFSIVLV"
     gene            1662..2375
                     /locus_tag="P5573_03000"
     CDS             1662..2375
                     /locus_tag="P5573_03000"
                     /inference="COORDINATES: protein motif:HMM:NF016269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HRT27066.1"
                     /translation="MEDMVSKKMLTRTLFLVVVLSFGLTLWTASVFADDKTEANQLVE
                     KAKMTFENCVKDQNMRVMVDLLKDAKGVFIAPQVLRGAFVVGASGGSGVFVARDKDNR
                     WSGPAFYTLGGVSVGFQVGGDASEVILVAMTERGVKAFQSTNFKLGADAGIAVGPAGA
                     GIAAQTANLSADIVSFSRAKGLYVGLSLDGAVVAARDDLNRAFYGKKVKPADILTKPG
                     FSNPKASGLLQEVARAAKK"
     gene            2390..3436
                     /locus_tag="P5573_03005"
                     /pseudo
     CDS             2390..3436
                     /locus_tag="P5573_03005"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
     gene            complement(3506..4546)
                     /locus_tag="P5573_03010"
     CDS             complement(3506..4546)
                     /locus_tag="P5573_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HRT27067.1"
                     /translation="MKAMLLKKISSVAENREPLEAADIPTPVPGKREILVRVAVCGVC
                     HTELDEIEGRTAPPRLPVVPGHQVVGTVAASGPGASRFSVGDRVGVPWIHSACGECAW
                     CRSGRENLCPDFQATGRDVNGGYAEYMTVREDFAHPIPSVFTDAEAAPLLCAGAVGYR
                     SLMLTGVENGQSLGLTGFGASGHLVIMTAKHLYPRVRVFVFARSAEERRFAKELGADW
                     AGETEAEPPERLDSIIDTTPAWKPVVEALKNLKPAGRLVINAIRKEEADKDRLLRLDY
                     STHLWLEKEVKSVANVTRSDVGEFLALAAEIPIRPEVQEYPLEEANRALVELKERKIR
                     GAKVLRIRQPSP"
     gene            4840..5172
                     /locus_tag="P5573_03015"
     CDS             4840..5172
                     /locus_tag="P5573_03015"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HRT27068.1"
                     /translation="MPETPREFMEFLQTRFKEKPEAGKDINAIYQFVLEGENGGDWWI
                     DLTESPGQVGEGRNPSPGCTITMAAPDFVAMMKKTANPVVLFMSKKLKVDGDLALSLK
                     LQGVLNLL"
     gene            complement(5330..>5948)
                     /locus_tag="P5573_03020"
     CDS             complement(5330..>5948)
                     /locus_tag="P5573_03020"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRT27069.1"
                     /translation="GTKAFVNCSVSLKRDSSGNPVGFYGILHDITERKRMEEEIRALA
                     IIDQLTGLYNRRGFITLAEQQMRNVERSKKGLFLIFADLDNLKKINDTLGHRAGDERL
                     VQLAGILRKTFRKADVIARIGGDEFAVLAEDNMLANPDTIRSRLQRQIDLYNAKAGKD
                     TEVLSLSIGIVRYNPEEPETVDNLMSRADTRMYEHKKGKAPDLER"
BASE COUNT         1114 a         1682 c         1861 g         1291 t
ORIGIN      
        1 atgacggcct ggaatcacag ggggctggtg gaaagccggg attcccctta tgggcgtgtg
       61 accgtcacaa aactcgacgg gcaggtgtcc gttttcgaga acgacgccat cgccttcgag
      121 acccagggga cggaggcgga gaccttcgcg aatctcgcgg ctctccagca tccgggcccg
      181 gagtccgttc tgatcctggg cgggggggtc gaggggacgg taaaggagat tctcaaacaa
      241 tccccgaagc gggtcgatta tgtcgaactc aaccccgtga tggtggaagc tgtcctgccg
      301 cacctccccg cggacatccg ccggtcgttc gaacagccgg gcgtgcggct gatcgtggac
      361 gacccgcgga agttcctccg gggaagcggc cggtacgatc tcatcctgat cggtatgccg
      421 gaaccctctt cgggacagtc caacaggttt ttcacgagag agttcttctc cctttgcgcg
      481 gagcggttga accctctggg ggtcgtcggc ctgaggctgc gctccgccga aaatttctgg
      541 acgcctcagt tgcggcgccg gacggcgagc atctaccacg ccctggcgtc cgtgttcccg
      601 tcggttctcg ttcttcccgg ggccaccgac atcatcacgg catcgaggtc tccgcttccc
      661 accgcgcccg aagttatggt cgaaaggctg gagcgacgga aggtcgaggc caggctcgtg
      721 tctgcgcctt atatttgtta tctcttcaca aacgaccggt tcgccgaggt caacggcacc
      781 ctcagggcgt cctcagtaca gccgaatacg gactcaaggc cggtgggcta tcagtacgcg
      841 ctgatgacct ggctcgcgaa atttttcccc cagctcgcac tgccggaccc ggtggagtac
      901 gtggaatttt acgttgcgaa agggatggga tactgggccg gcgccgtcgt actcaccatc
      961 tttctcctgg cgcggctccg cccttcgtgg cgcaacgcca tgcttgtttt tacggcgggg
     1021 ttcctgggga tggtgctcga gaccgttctg atcctccatt accaggtcaa gtccggtgtc
     1081 ctttatcagg atatcggcat tttgttgacc ctgttcatgg ccggtctcgc actgggttct
     1141 gctcttttcg acagaggggt gttccgaagg gacagacgca ggagaagcgc actatggatc
     1201 ggcgctggca tgatggcact cttcggcctg tactgcctgc tcatcatggt tcaggtcaag
     1261 gccgacaatc tggccgggtt gacgacgacg gcaggcctcc tgggaggatt aggagtcctg
     1321 gtcgcggggg tcttcgggta cgcaagctta cacgaccgtc aggaccagga cagggtggtc
     1381 tctcctctct acgcggccga cctgatcggc ggctgcctgg gttcgatcct tgcgagcctc
     1441 gtgatgatac ccttactcgg cctcgacgtg accttgcagg ggatgacgat gcttgccctg
     1501 ttttccatcg tcctcgtgtg agagcgatgc agggagcatt gaggtctgtg gaatcgggag
     1561 ccggcggctt ccttgtggga agaaaatcag tttgcctttt cccctgtgcg tatgcgatat
     1621 gaggccgaaa ataccgacag agttgcgaag gaggaaggaa gatggaagat atggtgtcga
     1681 aaaagatgtt gacgaggacc cttttcctgg tagttgtact ttcttttggg ctgacccttt
     1741 ggaccgcttc ggtcttcgcc gacgacaaga cggaggcaaa ccagcttgtg gaaaaggcga
     1801 aaatgacctt tgaaaactgc gtgaaggacc agaacatgag ggtcatggtt gacctgttga
     1861 aagatgccaa aggggtgttc atcgcgcccc aggtcctccg tggcgccttc gtagttgggg
     1921 cgtcgggtgg aagcggcgtc tttgtcgcaa gggataagga caacaggtgg tccggtcccg
     1981 ccttttacac gctgggcggc gtgagtgtgg gattccaggt cggcggtgac gcctcggaag
     2041 tgatccttgt cgccatgacg gaaaggggag tcaaggcctt tcagtcgacg aacttcaagc
     2101 ttggggcaga cgccggtata gcggtcggcc ccgcgggggc gggcatcgcc gctcagacgg
     2161 cgaacctgag cgcggacatc gtgagctttt cccgcgcgaa agggctttac gtaggcctct
     2221 cactcgacgg cgcggtagtt gctgcacggg acgacctcaa cagggctttt tacgggaaaa
     2281 aggtgaaacc tgccgatatc ctgaccaaac ccgggttcag caacccgaag gcgtcggggt
     2341 tgctccagga ggttgcccgg gcggcaaaga agtagaacaa cataaggaaa ggttttctat
     2401 gaagacgcgc ataacggagc ttttcgggat tgaataccct attctccttg cggggatgag
     2461 ctggatcagc gtgccgaaga tggtggcggc ggtctcgaac gccggggggt tgggtctcct
     2521 tgccaccggg ccgctgagtg cggaggagac ccgcaaggcc gtccgggaaa tcagggaatc
     2581 gaccgacaaa ccgttcgggg ccaacctggc gttgatgttt cccggagctg tcgagaacgc
     2641 gaaggttctt ctcgaggaaa aggtgccggt catcaacttt tctctcggca agggcgactg
     2701 gctcgttaag cgggcgcatg aatacggcgg caaggtcgtg gccaccgtcg tcggggcccg
     2761 ccatgccctg agggcgcagg attacggatg cgacgccgtg atcgccacga gccatgaagc
     2821 ggcggcccac ggcgaagcgc tcaccacctt catcctcata ccgaccctct gcgacgtcct
     2881 gaaaataccg gtcatcgcgg cgggcgggat cggcgacggc agagggcttg cggcagccct
     2941 cgccctgggt gccgagggtg tttctatggg cacccgtttc atgacaacga aggaaagtcc
     3001 gctccataac gtctacaaga gattatccat agaaaaggac gcgacggata cgctgttttc
     3061 cgaccgtttt gacggtttgt tctgccgtgt gatgaagacc gaagcggccg agaaggccat
     3121 caagcggggg ctcgacctgc cggcggcatt cttcaacgcc agggagatta cccgtcagct
     3181 caacgtcccg ttcttgaaag tccttgcggg ggtggcggcc tcgggttgga aaacggcgaa
     3241 acagatggcc ttcctggcca atgccttcaa ggcgatccgt gtggccaccg aggagggaga
     3301 cacgaaagag ggcgtcttgc ccgccggtca ggtcatggga ttgattcacg acgaaccgac
     3361 ggtggcggag gtcgtcgaac gcacagtggc ggaggcgaaa gaggtacagc gtaggctggc
     3421 cgaaaagctg ggttgaattc tacggcggga aaacaagaat tcggtcggcc tatgttacgg
     3481 ttatgggcct taaaccgcac cggttttaag gtgaaggctg tcggattcgg aggaccttcg
     3541 caccccggat ttttctctct ttaagctcta caagagcccg gttggcctct tccaggggat
     3601 attcctggac ctcgggcctg atcggtatct ctgcggcgag ggcgaggaac tcgccgacgt
     3661 cgctcctcgt cacgttagca acgctcttca cctccttttc cagccagaga tgggtggagt
     3721 aatcgagccg gagcaggcgg tccttgtcgg cttcctcctt acggatggcg ttgatgacca
     3781 gccttccggc gggtttcagg tttttcaagg cctcgacgac cggtttccag gctggggtag
     3841 tgtcgatgat actgtcgagc ctctccggag gttctgcctc cgtttcgccg gcccagtccg
     3901 ccccgagctc ctttgcgaat cgcctttcct ctgcgctccg ggcgaagacg aagacgcgga
     3961 cgcggggata aaggtgtttc gccgtcatga tgacgagatg gcccgaggcc ccgaaaccgg
     4021 tcagccccag gctctgcccg ttctcgaccc ccgtcagcat cagggaacgg taaccgacgg
     4081 ccccggcgca caggaggggt gccgcttcgg cgtcggtgaa gacggacggg atggggtggg
     4141 cgaagtcctc ccggacggtc atgtactcgg cgtagccgcc gttcacgtcc cggcccgtcg
     4201 cctgaaaatc gggacagagg ttttctcttc ccgaccggca ccaggcgcat tctccgcagg
     4261 ctgaatggat ccagggaacc ccgacgcggt ccccgacact gaaacggctg gcgccgggac
     4321 cgcttgccgc caccgtgcct acgacctggt ggcccggcac gacgggaagc ctgggaggcg
     4381 ccgttctgcc ttcgatttca tcgagctcag tgtggcagac accgcagaca gcgaccctga
     4441 ccaagatctc ccgctttccc ggtaccggcg tggggatgtc cgccgcctcc agcggctccc
     4501 ggttctcggc gacggacgat atttttttca agagcatggc cttcataggt ttctcctgtt
     4561 gtgcagtagg ccggttcacc ttgcagtaat tcccgcgaag tcgcggcgca accctctgac
     4621 ttctctaacc catttatata acagtcgatt caatagggca atcttgaatt ttttcccttc
     4681 cgaaaggcac gcttgactga agccccgcct cgtgtcttcc ctcagggacg ttccgccggg
     4741 ggtattcgca gaactcggtt cttctttgat gttcttgaca ggttgcggtg gtcgctggta
     4801 agtacagcca atattactta tgaaggagga tagcccctga tgccggagac acccagggag
     4861 ttcatggaat ttttgcagac tcggttcaag gagaaacccg aagcgggtaa agacataaac
     4921 gccatctacc agtttgtcct cgaaggcgag aacggcggtg actggtggat tgaccttacc
     4981 gagagccccg gccaggtagg cgaggggaga aacccgtcgc cgggatgcac catcaccatg
     5041 gccgcccccg attttgtggc catgatgaag aagacggcta accccgtcgt cctcttcatg
     5101 tccaagaagc tgaaggtcga cggtgacctc gcgctttccc tgaagctcca gggcgtcctc
     5161 aacctgctgt gagcgccgcc aaaaagaaga aggcccgtat cttaggcgcc ttcgactttg
     5221 ctcaggagac cctttctcga tagtcggttt gagggttgac gatgcggcag tgtcttacgc
     5281 ggttggtgtt ttgaatcttg ccgctttggg agtaagcggc cgcgggggtt catctttcca
     5341 ggtcgggcgc cttgcccttt ttgtgttcgt acatccttgt gtccgctcgt gacatcaggt
     5401 tgtccacggt ctccggttct tcgggattgt aacggacgat gccgatactc agggaaagaa
     5461 cctccgtgtc tttccccgcc ttggcgttgt ataggtcgat ctgccgttgc aagcgactcc
     5521 ggatcgtgtc ggggttggcc agcatgttgt cttcggcgag gaccgcgaat tcgtcacccc
     5581 cgatgcgggc gatgacgtcg gctttccgga atgttttcct aaggataccg gccagttgga
     5641 ccagtctttc atcgcccgcc ctgtggccga gggtgtcgtt gattttcttg aggttgtcga
     5701 ggtcggcgaa gatgaggaac aacccttttt tcgacctctc gacattccgc atctgctgtt
     5761 cggcgagggt tatgaatccc cttctgttgt ataagcccgt caactggtcg attatggcga
     5821 gggctcgaat ctcttcttcc atcctcttgc gctcggtgat gtcgtgcagt atcccgtaaa
     5881 acccaacggg gttgccggag gaatctctct ttaacgaaac cgagcagttc acaaaggcct
     5941 ttgtaccg
//
LOCUS       DAOTMX010000070         5946 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_11394_length_5946_cov_3.448141, whole genome shotgun sequence.
ACCESSION   DAOTMX010000070 DAOTMX010000000
VERSION     DAOTMX010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5946
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11394_length_5946_cov_3.448141"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="P5573_03025"
     CDS             complement(<1..845)
                     /locus_tag="P5573_03025"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HRT27070.1"
                     /translation="MAKGKTAAGGKNNGADLGYEARLWQMADALRGSMDAAEYKHVVL
                     GLIFLKYISDAFEELHARLESERAQGADPEDRDEYIAQNIFWVPPESRWSFLQAQAKQ
                     PAIGRLVDDAMEGIERDNPALKGVLPKDYARPTLDKARLGQLIDLVSNIQVGNEDARA
                     RDVLGRVYEYFLSQFASAEGKKGGEFYTPRCVVQTIVEMLEPGKGRVYDPCCGSGGMF
                     VQSERFIEDHQGRRSDIAIYGQERNHTTWRLCRMNLAVRGIDADIRWNNEGSFEDLLY
                     KEPKIR"
     gene            1155..1502
                     /locus_tag="P5573_03030"
     CDS             1155..1502
                     /locus_tag="P5573_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27071.1"
                     /translation="MNKGTRMDAFAIRVLRAFAWVNLFFFIAASILILQEYGFTFVTD
                     EELFYDYTEKLLNPFALLVSLSSFLFGIFGWAFCLVVAHMAENLSALRRSFTPSSVPP
                     SAPTPAEDQQNTT"
     gene            1499..2485
                     /locus_tag="P5573_03035"
     CDS             1499..2485
                     /locus_tag="P5573_03035"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRT27072.1"
                     /translation="MNRASLTEKIVLLAVAAGVLCGCVSVQRYGELKKELGECKKQSE
                     AVGNEAKLLKQKNEDLTRELQQLRQPVEYYFKTGMDHYNNKQYAKALEYFEKIVDRYP
                     TDGLAPEARNKITELAAISSANYEKIVKAAEHAHDPRNRLEIVDREREARFLTKEDDA
                     RLLRKRDEYYDEWKYLDGAAKNILVEDDPTKSLRVYRTTRSTLRRAGNDKSFYFEIYI
                     VQHYSGKKDFRLRSRYVGDKWISYDSLTLKGDNGTQADIYCKYPEKMSTMAEDRFFEW
                     SDNDIEDDKVLKLAKSQTVTVLFNGGYKYSFELSDEQMTSLREIVKKYQFLK"
     gene            complement(2489..3556)
                     /locus_tag="P5573_03040"
     CDS             complement(2489..3556)
                     /locus_tag="P5573_03040"
                     /inference="COORDINATES: protein motif:HMM:NF012688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HRT27073.1"
                     /translation="MAGERFNIQSPKQLQQILFEKLALPRGKRTKAGFSTDFEVLLSL
                     SQNHELPAEILAYRSIAKLKSTYVDALPGLINPETGRVHTSYNQTGTETGRLSSSNPN
                     LQNIPTKTPEGRRIRQAFIAPPGYKILSADYSQIDLRVLAHLSGDRNLIEAFHADEDV
                     HSRTAADVFGVFPEMVTPGMRRQAKVVNFGVIYGMSPFGLAKELGVSQSVARAYIDGY
                     FARYSGVREYIERTLAEARRKGYVTTLMNRRRYLPEINSSNPAVRQFAERTAINTPIQ
                     GTSADLIKLAMVRIYREMERRGFSSGMIMQVHDELVFEVAEDEIESAVELIKEGMEGV
                     ASLRVPLKVGVSWGDNWDEAH"
     gene            complement(3853..5115)
                     /locus_tag="P5573_03045"
                     /pseudo
     CDS             complement(3853..5115)
                     /locus_tag="P5573_03045"
                     /inference="COORDINATES: protein motif:HMM:NF014759.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
     gene            complement(5143..5589)
                     /locus_tag="P5573_03050"
     CDS             complement(5143..5589)
                     /locus_tag="P5573_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HRT27074.1"
                     /translation="MDGVDRKILNLIQKEFPVTAEPFKEIGASVGVSEDEALERVRRL
                     REEGIIRRIGAVFDPGKLGFVSALCAARVPEEKIRSFVETVNVLPGVTHNYRRNHEYN
                     VWFTVIAPSEESLEESLAGIKENSGVVDILTMRAVRTFKVDASFEL"
     gene            complement(5589..>5946)
                     /locus_tag="P5573_03055"
     CDS             complement(5589..>5946)
                     /locus_tag="P5573_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HRT27075.1"
                     /translation="REKGAGQSGNPFSAMTRGCMGGSSFCFISHTGRVQPCGYLEIDC
                     GSVRERPFREIWEGSEIFRDLRDLRRYKGKCGRCEFLRVCGGCRARAYETTGDYLAEE
                     PFCVYEPGEQQRAKAD"
BASE COUNT         1210 a         1831 c         1653 g         1252 t
ORIGIN      
        1 cggattttag gttctttgta aagaagatct tcaaaactgc cttcattgtt ccaacggatg
       61 tcagcgtcaa tcccgcggac cgctaagttc atccggcaga ggcgccaagt ggtgtggttg
      121 cgctcttggc catagatggc aatgtcggat cggcggccct gatggtcctc gatgaatcgc
      181 tcagactgaa cgaacatgcc gcccgacccg cagcacgggt cataaacgcg tcccttaccg
      241 ggttcgagca tctcgacgat ggtctggacc acgcagcggg gcgtgtagaa ctcgccgcct
      301 ttctttcctt cggcgctcgc aaactgggaa agaaagtatt cgtagacccg gccgagtaca
      361 tctcgggcgc gtgcatcctc gttgccgacc tggatgttgc tcaccaggtc tatgagttgg
      421 ccgagccgcg ccttgtccaa ggtcgggcga gcgtaatcct tcggcagcac gcccttgagg
      481 gcggggttgt cgcgctcgat gccttccatg gcgtcgtcca ccaggcggcc gatcgcgggc
      541 tgcttcgcct gcgcctggag aaaggaccag cgggactcgg gcggcaccca gaagatattc
      601 tgggcgatgt attcgtccct gtcctccggg tcggcaccct gcgcccgctc cgattcgagg
      661 cgggcgtgca gttcctcgaa ggcgtcggag atgtacttga gaaagatgag ccccaaaacg
      721 acgtgcttgt attccgccgc gtccatgctg ccgcgcaggg cgtcggccat ctgccagagc
      781 ctggcttcgt aaccgaggtc ggcgccgtta tttttcccgc ccgctgcggt ctttcctttt
      841 gccatctcaa ccctcttccc tgatattcgc tgccgaaata acaagtctac accaagccca
      901 ccccgggacg ccttccagga acaaacgtct gcagggcggg aaggccctct tcctggagtg
      961 gaaaggcacg ggacacagcg caaccggaaa ccaaccgtcc gttacaggtt tatggcatta
     1021 aacaccaaac cagtcaagat tccgctgaat ttgtttgtgt cttgttcaag agtatgataa
     1081 aggctgctcg attttataag ctgtgactct ggtcgcgaac cggaaataca cacatcgggg
     1141 ttggtttaaa cgccatgaat aaaggtacgc gaatggatgc tttcgccatc agggtgctca
     1201 gggccttcgc ctgggtgaac ctgtttttct tcatagcggc ttctattctt atcctccaag
     1261 aatacggctt caccttcgtg acggatgaag aattgttcta tgattacacg gagaaactcc
     1321 tgaatccctt cgccctcctg gtcagcctct cctcctttct cttcggaatc ttcggctggg
     1381 ccttctgcct tgtcgtcgcc catatggcgg aaaacctgtc ggccttgaga aggtcgttta
     1441 caccgtcgtc tgttccccct tctgcgccca cccctgccga ggatcagcag aatacaacat
     1501 gaacagggca tcgttgaccg aaaagatcgt cctccttgcc gtcgccgcgg gtgttctctg
     1561 cgggtgcgtc tcggtccaga ggtacggcga actcaaaaaa gagctcgggg agtgcaagaa
     1621 acagagcgag gccgtcggga acgaggccaa gctcctcaag caaaaaaacg aagacctcac
     1681 gcgggaactg caacagctga gacagcccgt agaatactac ttcaaaacgg gcatggatca
     1741 ttacaacaac aagcagtacg cgaaggcact ggaatacttc gaaaaaatcg tcgaccggta
     1801 tcccacagac ggccttgccc ccgaggcgcg gaacaagatc accgagctgg cggctatctc
     1861 ctcggccaac tatgaaaaga tcgtcaaggc cgcggagcac gcccacgacc cccggaacag
     1921 gctggagatc gtagaccggg aaagggaagc gagatttctg acgaaagaag acgacgcaag
     1981 gcttctcagg aaaagagacg agtattacga cgagtggaaa tacctcgacg gcgccgcgaa
     2041 gaacatcctc gtggaggatg acccgaccaa atccctgagg gtctaccgga cgacaaggtc
     2101 aaccctcaga agggcaggta acgacaagag cttctacttc gagatttaca tagtccagca
     2161 ttattccggc aagaaagact tcaggctcag gtcgagatac gtcggcgaca aatggatcag
     2221 ttacgacagc ctgaccctga agggagataa cggcacccag gcggacatct actgcaaata
     2281 cccggaaaag atgtccacta tggcggagga ccgattcttt gagtggtccg ataacgacat
     2341 cgaagacgac aaggtcctca aactggccaa gtcgcaaacg gttaccgtcc tcttcaacgg
     2401 gggttacaaa tacagcttcg agctttcgga cgaacagatg acgtccctgc gggaaatcgt
     2461 caagaaatac cagttcctga agtgaatatc agtgggcctc gtcccagttg tcgccccatg
     2521 acacgcccac cttgaggggt actctgagac tcgccacccc ttccatcccc tctttgatca
     2581 attccacggc gctttctatc tcatcctcgg ccacctcaaa gacgagctcg tcatggacct
     2641 gcatgatcat ccccgaggaa aacccccttc tctccatctc cctgtagatc cgaaccatcg
     2701 cgagcttgat gaggtccgcc gaggtgccct ggatcggggt gttgatggcc gttcgctccg
     2761 cgaattgccg gaccgcgggg ttggagctgt ttatctccgg gagataccgg cgccggttca
     2821 tcaacgtggt gacataaccc ttccgcctgg cctccgccaa agttctttca atgtactcac
     2881 gcacgccgct gtaacgggcg aaatagccgt cgatgtacgc ccgggcgacc gattggctga
     2941 ccccgagttc cttcgcaagc ccgaaggggc tcatcccgta gatcacgccg aagttgacga
     3001 ccttcgcctg ccgcctcatc ccgggcgtca ccatctccgg gaagacaccg aagacgtccg
     3061 ccgccgtccg gctgtggacg tcctcgtcgg catggaaggc ctcgatcagg ttcctgtcgc
     3121 cggagagatg ggccaacaca cgcaggtcga tctgcgagta atcggccgaa aggatcttgt
     3181 accccggcgg cgcgatgaag gcctgtctta tcctccgccc ctcgggggtc ttcgtcggga
     3241 tgttctggag gttcgggttg ctgctcgaga ggcgtcccgt ctcggttccc gtctggttgt
     3301 aagatgtatg gacccgcccc gtttctggat tgatcaaacc gggaagggcg tctacatagg
     3361 tggatttgag tttcgcgata ctccggtaag cgaggatctc cgcggggagc tcgtggtttt
     3421 gggagagcga gaggaggacc tcaaagtccg tggaaaaacc ggccttcgtc ctcttccccc
     3481 gggggagagc gagtttctcg aagagtatct gctgaagctg cttgggcgac tggatgttga
     3541 accgctcccc cgccaaccgg tatatctttt ccccgaacgt gaaagcagtt cctcgaactg
     3601 gcgggacatt tcccggagga tgcccacgtc cagcaggacg ccccgcctct ccatggaggc
     3661 gaggacttct atgagcggca tctccatccc gtgaaaaagg tccccgaacc cgtccttctc
     3721 gacctgatgg gacatcgtca gggacagccg ctgcacggcg tcggctcgcc ggcaggcatg
     3781 ggacagggcc tgttcaacgg gggcggaggc gggcggcgcc tgcagaacgg ggagctcatg
     3841 gtccagaccc tccctgacga gcgcttcgag gccgtagtcc ttcttcgagg gattgaggac
     3901 gtaggccgcg accatgacgt cgaagccgag gcccttcaga gagacgccct tgcgggagag
     3961 caggatcacg tcgtttttca ggtcatggcc gcgcttctcc aatcgttcgt ctgcaagaaa
     4021 gggcgcaagg gcccgcaata cccactcctg cggcaactgt tccggcgccc cggggtaggc
     4081 gtggcccatc gggatgtaga aggcctcccc ttccttgaac gagagggcca tcccgacgat
     4141 gtcggcctgc atggcggcat ccgacgtctt ctccaggacc agggacacct ggtcggcctc
     4201 tttcagcctc tcggcgaggg cgcggagttc ctcctccccg actacaagtc tgtagtcacc
     4261 ctccaggccc tcgtcctcga ccttcaattc cttgaggagg gacgcaaact cgagttcctt
     4321 gaagatgtcc ttgaggaggt gcgggtccgg cccccgatag cgcgcctttt ccaggtcgaa
     4381 ttccacgtcc gcatcggtca tgagcgtcac cagttgcctc cccagcctcg cctggtccgc
     4441 atatcgcccg atgctttcct tgagccgtgc gttctttatc ccgtcgagat gtcccattac
     4501 ttcttcgagg gagccgaact cggcgatcaa cctctgcgcc gtcttgtccc cgatgccggg
     4561 cacacccggg atgttgtcgg aggcgtctcc cgtgagcgcc agtacatcga cgaccttttc
     4621 cggcgccacg ccgaaacgcc ctttcacgcc gtcgatgtcg tagaccctat ccttcatcgt
     4681 gtcgacaacc gtgatgtctt ccgacaccat ctgcagaaaa tccttgtcac ccgagacgat
     4741 gaagacctta atcccctttt ctgcgtattt tttcgccaag gtcgctatta agtcgtccgc
     4801 ctcgatcccc tccttttcga tcacggggat agagaacccc ctgacaacat ccttgatgaa
     4861 ggggatctgc gggatgaggg cgtccggggt cggggcgcga ttggccttgt acgattcgta
     4921 gacttcgtcg cggaacgtcg gccctttgag gtcgaaggcg acggcgatgt agtcgggctt
     4981 ccgatcccgc aagagcttca tcaacatcgt cgcgaagccg taaacggcgt tcgtcggaaa
     5041 acctttagaa ttggagagat gcctgatggc atagaaggcc cggaagacgt aattgctccc
     5101 gtctaccagg tacattgtgg gtttcttctc ttcttcgctc atctacagct caaagctcgc
     5161 gtcgaccttg aaggtgcgca ccgcacgcat agtcaggatg tcgacgaccc cgctattttc
     5221 cttgatcccc gcgagggact cttccagtga ctcctctgag ggggcgatga ccgtaaacca
     5281 gacgttgtat tcgtgattcc ggcgatagtt gtgggtcacg cccggcagga cgttaaccgt
     5341 ctcgacgaaa gacctgatct tttcctccgg gacgcgggcg gcgcagagag cgctcacgaa
     5401 gccgagttta ccggggtcga agaccgcccc gatcctgcgg attattccct cttctctcag
     5461 tcttctcacc cgctcgagcg cctcgtcttc ggagaccccg accgaggcgc cgatctcctt
     5521 gaacggttcc gccgtcacgg ggaattcctt ctggataagg ttcaggattt ttctgtcaac
     5581 gccgtccatc aatcggcctt tgccctttgc tgctctcccg gttcatagac gcagaacggc
     5641 tcctccgcca gatagtcgcc cgtcgtctcg tacgcccgcg cccggcaacc cccgcagacc
     5701 ctgaggaatt cgcagcggcc gcacttgccc ttgtaacgcc tcaagtcacg caggtcccga
     5761 aagatttccg acccctccca tatctcccgg aaaggccttt ccctgacgct gccgcagtct
     5821 atctcgaggt acccgcaggg ctggacccgg cctgtatgcg aaataaagca gaaggaacta
     5881 ccgcccatac agcccctggt catcgcggaa aagggattcc cggattgccc cgcccctttc
     5941 tccctt
//
LOCUS       DAOTMX010000071         5942 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_11407_length_5942_cov_3.251401, whole genome shotgun sequence.
ACCESSION   DAOTMX010000071 DAOTMX010000000
VERSION     DAOTMX010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5942
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11407_length_5942_cov_3.251401"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..1961)
                     /locus_tag="P5573_03060"
     CDS             complement(96..1961)
                     /locus_tag="P5573_03060"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HRT27076.1"
                     /translation="MSNLLVNARDQKFVLYEQLDIERLLSTEKFADYSKDTVDMILTE
                     AEKMALEVLEPTYATGDKEGVKFEGGKVFVPSGFYEAYKKFIEAGWQCCMKSPEVGGQ
                     GMPITISTACFEYFDAANCPLIMYPMLTTGAASLIEHFGTEEQKNKYMYRMFSGEWGG
                     TMCLTEPGAGSDVGALKTSARRLPDGTYSITGTKIFISCGDHNMCENIVHPVLARIEG
                     DPPGTGGISIFIVPKYRVNKDGSLGEFNDVRTGNVEHKMGIKGSATCTLNFGDDGQCI
                     GELLGKEREGLRIMFHMMNEARLEVGMQGLALASVAYEHAVDYAKGRIQGTAIWDMKN
                     PDAKSVPIIQHPDIRRMLLWMKAHVEGLRALNYYAAYCIDMSQVAEDPEERERWHGMI
                     ELLIPICKAYSSEKAFQVCSRAIEVYGGYGYCSEYPVEQYLRDCKIASIYEGTNGIQA
                     LDLVGRKLGMRKGANVKNLSYDMMKDIAALRKVEELRSYNAFLEEAGNSLSDLTLYFS
                     QVGKGAGFLVPILYAQPYLEIFGDVVIGWLLLKQAAIAAGKLGAIYAEKGAVGSRAKQ
                     RALVHENADVAFYQGKIATAKFFALTALTTVKARCGAIKLGDKTPIEMADESFAS"
     gene            complement(1994..2674)
                     /locus_tag="P5573_03065"
     CDS             complement(1994..2674)
                     /locus_tag="P5573_03065"
                     /inference="COORDINATES: protein motif:HMM:NF019959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRT27077.1"
                     /translation="MGLEERRSREKEVRRNAILKAARKLAFEKGFKSITVASIAKKAE
                     LSKGAVYLYFDSKEEIYVQILLKDIEKFHASLLDIYECGGTAGDMLRRFADVYIDFFL
                     NDRELFRIFMTFMLHIEEMGFSEEIVSQLIGATNKTIDIIEKILKLGIENGEFPENFN
                     IRQGQNAVWGLLNGVIALHLFTGKEATREQRIRTTARFGLDLFLSGLGMRSERGVGGT
                     PAMGHSTQ"
     gene            2866..4992
                     /gene="recG"
                     /locus_tag="P5573_03070"
     CDS             2866..4992
                     /gene="recG"
                     /locus_tag="P5573_03070"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HRT27078.1"
                     /translation="MNAKDAQAGTDGVAMPVRYVKGVGPKTAALLEKKGVRTVEDLLY
                     FAPIRYEDRRRIDRVSEITETRRAVVVGVVRKASAVYYRRVRVFEAVVDDGSGQVVLK
                     WFRGNDSYLRWSFRPGRKIIFFGELKVSPFGKEMAHPDFETIDEGIDDENLHFGRSVP
                     VYSETEGLQQKQLRRVVRNALDTFSQCVGSPIPAEIRSRRGLADIPSALKDIHFPAID
                     ADVGLYNERKSLAHRSLVFDELFFFQLALALKKGVRDRAPGIAFRRRGKLVESFFGML
                     PFELTAAQRRVFDEVEKDMSDGRPMQRLLQGDVGCGKTVIAMAAVLRAVENGYQGAVM
                     APTEILAVQHHQNMRRWAEPLGLRIGLLTGSLSKKDRLDTLDRVASGRLDIVIGTHAL
                     IQDHVSFKGLGLVVIDEQHRFGVAQRQKFTEKGANPDVLVMTATPIPRTLAMTVYGDL
                     DVSVIDEMPRGRARVKTKVFSERQRDRVYEIVRSEVAKGGQAFVVYPLVAGSETLDLR
                     DATRMAGHLQRDVFPGFRVGLVHGRMKGEEKERIMSEFSGGGLDILVSTTVVEVGIDV
                     PQASLMVIEHAERFGLSQLHQLRGRVGRGNRRSYCILMASSTGTKVSSRRLRVMEETN
                     DGFLVAEEDLAIRGPGEFMGTRQSGLPEFRVANIFRDLGIMDEAREDAFSVIERDPGL
                     ARPENEGLYRELMRRWSDRIGYAKTG"
     gene            5061..>5942
                     /locus_tag="P5573_03075"
     CDS             5061..>5942
                     /locus_tag="P5573_03075"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HRT27079.1"
                     /translation="MKKIYAGLIGFGNIGTGVVKLLNMNRGLIEDRLGASIILKKIAD
                     IDIETPREVAVDRSILTTEAADILGDPEISIVIELVGGYQPALSFILEAVRAKKHVVT
                     ANKALLATYGNEVFRAAEKEDVDIGFEASVGGTIPVIKTLKEGLVANRIKSVLGIMNG
                     TSNYILTKMSDEGQAFADVLKEAQRLGFAEADPTFDIEGIDTAHKLAILLSLSYGRKV
                     EIESICREGVSGIAQKDIEFAKELGYKIKLLAIARERGEEVEARIHPTMIPFNHLLAN
                     VNGNFNAIHFVGDASDSV"
BASE COUNT         1263 a         1636 c         1732 g         1311 t
ORIGIN      
        1 ttgatttcga aaggcatcgt ttcctccttt ttgctgtgat tgcccgtcgg cggttcgcgc
       61 tcgcggcggg acgtcaaagg gtttgctgca cggcgctatg acgcgaacga ttcatcggcc
      121 atctcgatag gggtcttatc cccgagcttg atggccccgc agcgcgcctt gaccgtagtc
      181 aaggctgtca aggcgaagaa cttcgccgtg gctatcttgc cttggtaaaa ggcgacatcg
      241 gcgttttcat gtaccagtgc cctctgtttt gcccgggaac ctacggcacc cttttctgcg
      301 tagatggcgc cgagtttccc tgccgcaata gcggcctgtt tcagcaggag ccaaccaatc
      361 accacatcac cgaatatctc caggtacggc tgggcgtaaa ggatggggac gagaaagcct
      421 gcgcctttcc cgacctggct gaagtacagg gtaaggtccg atagggagtt gccggcctct
      481 tccaaaaagg cgttgtagga cctcagttcc tcgacctttc tcagcgccgc tatgtctttc
      541 atcatgtcat aggagaggtt cttcacgttg gcgcctttcc tcatgccgag cttccgaccg
      601 accaggtcca aggcctggat gccgttggtt ccctcgtaga tgctggcgat cttgcaatcg
      661 cggaggtact gctcgacggg atattcggag cagtaaccat agccgccgta aacctcgatc
      721 gccctcgaac agacttgaaa ggctttctct gaagagtagg ccttgcagat gggtatcaag
      781 agctctatca tcccgtgcca cctttcccgc tcttccggat cttcggcgac ctgcgacatg
      841 tcgatgcagt atgcagcgta gtaattgagc gcccgcaggc cttccacgtg ggccttcatc
      901 caaaggagca tccttcttat gtcggggtgc tggatgatcg gaacggactt cgcgtcgggg
      961 tttttcatgt cccagatggc ggtcccctgt attctgcctt tggcgtaatc gacggcgtgc
     1021 tcgtaagcga cagacgcgag agcgagcccc tgcattccca cctcgagccg ggcctcgttc
     1081 atcatgtgga acatgatcct caagccttcc cgttctttac ccagaagctc gccgatgcac
     1141 tgtccgtcgt cgccgaaatt cagtgtgcag gttgccgagc ccttgatgcc catcttgtgt
     1201 tcgacgtttc ctgtccgcac gtcgttgaat tcaccgaggc ttccgtcctt gttcaccctg
     1261 tacttaggga cgatgaagat ggatataccg cctgttcccg gggggtcgcc ttcgatgcgg
     1321 gcaagtacgg ggtggactat gttttcacac atgttgtggt ccccgcagga gatgaatatc
     1381 ttggtccccg tgatgctgta tgtgccgtca ggcaatcttc tggccgacgt cttcagcgcg
     1441 ccgacgtcgc tcccggcccc gggctcggtg aggcacatgg tgcctcccca ctcgccgctg
     1501 aacatcctgt acatgtactt attcttctgt tcctccgtcc cgaagtgctc gatgagggag
     1561 gcggctcccg tagtcagcat cgggtacatg atcagcgggc aattggcggc gtcgaaatac
     1621 tcgaaacagg cggtggagat ggttatcggc attccctgac cgccgacttc gggggacttc
     1681 atgcagcact gccagcccgc ttcaatgaac ttcttgtagg cctcgtaaaa cccggagggc
     1741 acgaacacct tcccgccctc gaacttaacc ccctccttgt cgcccgtcgc ataagtaggt
     1801 tcaagaacct ccagggccat cttttcggcc tccgtcagga tcatgtccac ggtgtccttc
     1861 gagtagtcgg cgaatttttc cgtgctgaga agccgttcga tgtcaagttg ttcgtacagg
     1921 acgaatttct ggtccctcgc gtttaccaac agattgctca taacctcatc ctttctctta
     1981 ttccctcact tattcattga gtcgaatgcc ccatcgccgg ggttccaccg actcctcttt
     2041 cgctcctcat tccgaggccg gagagaaaca aatcaaggcc gaaccgggcg gtcgtgcgga
     2101 tcctctgttc cctcgtcgct tcctttcccg tgaagaggtg gagtgcgatg accccgttca
     2161 aaagacccca gacggcattc tggccctgcc ttatgttgaa gttttccggg aactcgccgt
     2221 tttcgatccc aagttttaat attttttcga tgatatctat ggtcttattc gttgcgccga
     2281 tgagctggct tacgatctct tcggaaaaac ccatctcttc gatgtgcagc atgaaggtca
     2341 tgaatattcg gaagagctct ctgtcgttga ggaaaaaatc gatatagacg tcggcgaaac
     2401 gtctgagcat gtctccggct gtgccgccgc actcgtatat atccaacagc gaggcatgga
     2461 acttctcaat atccttcaag agtatctgaa cgtagatttc ttccttgctg tcgaagtaga
     2521 gatagacggc gcctttgctc aactccgctt tcttggctat acttgcgaca gtgattgact
     2581 tgaaaccttt ctcgaaggcg agtttccggg cagccttgag gatggcgttt ctccgcacct
     2641 ctttttcccg gctccttctc tcttcgagtc ccacatcgtc ctccggatat gactaagggt
     2701 cattgtttga ataatggtca cataatgacc gttggtcatt ctattaaaca tgatttttgg
     2761 tccatgtcaa gataaatcag gtatcatttt gaagagcccg aatcattgac aaaatcggcg
     2821 cgatacagga atatgactcg gtcatttaaa ccttgagggt aggggatgaa cgcgaaagat
     2881 gcgcaggccg gcactgacgg ggtcgccatg cccgtccgtt atgtcaaggg cgtagggccg
     2941 aagacggcgg cgctcctcga gaaaaagggg gtgcgcaccg tagaagacct gctctacttt
     3001 gcgccgataa ggtacgaaga ccgtcggcgt atagaccggg tctccgaaat cactgaaacg
     3061 cgcagggcag tggttgtggg cgtcgtccgt aaggcctcgg ctgtgtacta tcggcgggta
     3121 cgggtattcg aggccgtagt cgatgacggg agcgggcagg tcgttctcaa gtggttcagg
     3181 ggaaacgact catacctgcg gtggtctttc aggcccggcc gaaagatcat tttcttcggc
     3241 gagctgaaag tttctccctt cgggaaggag atggcgcacc ccgatttcga gacgattgac
     3301 gaaggaatcg acgacgaaaa cctccatttc ggcaggagcg tacccgtata ctcggagacg
     3361 gaaggccttc agcaaaagca gttgagaagg gtcgtccgta atgcccttga taccttttct
     3421 caatgtgtcg ggagtccaat cccagcggag atacgcagcc gccgtgggtt ggccgacatc
     3481 ccctccgcct tgaaggacat ccattttccg gctatcgacg ccgatgtggg cctttacaac
     3541 gaaaggaaat ctctcgccca ccggagtctc gtcttcgatg aactgttctt cttccagctc
     3601 gccctggcct tgaaaaaggg tgtccgcgat cgggcaccgg ggattgcctt ccggcggcgg
     3661 ggcaaacttg tcgagtcctt cttcgggatg ctcccttttg agctaacggc ggcacagagg
     3721 cgggtgttcg acgaggtcga gaaagacatg tccgacggga ggccgatgca gaggctcctc
     3781 cagggagacg tcggctgtgg gaagacggtc atcgccatgg cggctgtcct tagggccgtg
     3841 gaaaacggct atcagggagc cgtgatggcc ccgacagaga tactcgcggt gcagcatcac
     3901 caaaacatgc gaagatgggc cgaaccgctc ggcctgagga ttggtctcct gacaggaagc
     3961 ctatcgaaaa aagaccgcct cgacaccctc gacagggtcg cttccggcag gctcgacatc
     4021 gtcatcggaa cccacgccct gattcaagac cacgtctctt tcaagggcct tgggttggtc
     4081 gtcatcgacg agcagcatag gttcggcgtc gcccagcgcc aaaagttcac cgagaaaggg
     4141 gcgaacccgg acgttctcgt catgacagcg acgccgatac cgaggactct cgccatgacg
     4201 gtctacggcg acctcgacgt atcggtcatc gacgagatgc cccggggaag ggcacgggta
     4261 aagacgaagg tcttctccga gaggcagaga gacagggtgt acgaaatcgt gaggtcggag
     4321 gtggcgaaag gaggccaggc cttcgtggtc tatcccctcg ttgccggaag cgagaccctc
     4381 gacctcaggg atgccacacg gatggcgggg catctgcaac gcgacgtttt tccaggattc
     4441 agggtcgggt tggtgcacgg caggatgaag ggcgaagaaa aggagaggat catgtcggaa
     4501 ttctccggcg gcgggctcga catactcgta tcgacgacgg tagtcgaggt tggcatcgac
     4561 gtgccgcagg cctccttgat ggtcatagaa catgcagagc gtttcgggct ttctcagctc
     4621 catcaactgc ggggtagggt agggaggggc aataggaggt cctactgtat cctcatggcc
     4681 tcgtcgaccg gcacgaaggt gtcgtcacgg cggctgcggg tcatggagga aacgaacgac
     4741 ggcttcctgg tagccgagga agacctggcg atccgcggtc ccggcgagtt catggggacg
     4801 cgccagtcgg gactgccgga attcagggtg gcgaacatct ttcgggacct cggaatcatg
     4861 gatgaagcgc gggaagatgc cttttcggtc atcgaaaggg acccggggtt ggcgaggccg
     4921 gaaaacgaag ggctgtacag ggaactgatg agaagatgga gcgacaggat aggttacgca
     4981 aaaaccggtt gaatttgaag agataaaagg gtaaaaggca ggcacaaata tcggcaggtc
     5041 ggcgagtcag aggataaccc atgaagaaga tctatgcagg ccttatcggt ttcgggaaca
     5101 tcgggacagg tgtcgtgaag ctgctcaaca tgaacagggg cctcatcgaa gaccgcctcg
     5161 gggcgtcgat catcctcaag aagatcgccg acatcgacat cgagacaccg cgggaggtgg
     5221 ccgttgacag gtcgatcctc acaaccgaag ctgccgatat cctcggggac cctgagattt
     5281 cgatcgtgat agaactcgtg ggcggctatc agccggccct gtcctttatt ttagaggccg
     5341 tcagggcgaa gaaacacgtc gttacggcaa acaaggcgct cctggcgact tacggaaatg
     5401 aggtattccg ggccgccgaa aaggaagacg tcgacatcgg cttcgaagcg agcgtcggcg
     5461 gaaccatccc ggtaatcaag acgttgaaag aaggcctggt ggcgaatcgg atcaagtccg
     5521 tcctgggcat aatgaacgga acctccaact acatcctgac gaagatgtcg gacgaaggac
     5581 aggccttcgc cgatgtcctg aaggaggccc agcgcctcgg ttttgccgag gccgacccga
     5641 ccttcgacat cgaggggatc gacacggcgc acaagctcgc catcctcctc tccctctcct
     5701 acgggaggaa ggtcgagatc gaaagtattt gccgcgaggg agtatcggga atcgcccaga
     5761 aggacataga atttgcgaag gagttgggtt acaagatcaa gctcctggcg atcgcccgcg
     5821 aaagaggtga ggaggtcgag gcgaggatcc acccaacgat gatacctttc aaccatctgc
     5881 tcgccaacgt gaacgggaac ttcaacgcca tccacttcgt cggcgatgcc tccgactcgg
     5941 tc
//
LOCUS       DAOTMX010000072         5934 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_11423_length_5934_cov_3.846062, whole genome shotgun sequence.
ACCESSION   DAOTMX010000072 DAOTMX010000000
VERSION     DAOTMX010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5934
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11423_length_5934_cov_3.846062"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="P5573_03080"
     CDS             <1..282
                     /locus_tag="P5573_03080"
                     /inference="COORDINATES: protein motif:HMM:NF023390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HRT27080.1"
                     /translation="LVRAPGGLRRRQHGKARLLFAMEKGVKGLEDDSPWEPGRFAARH
                     GGEVEKLAVAGEGQWGEWAQRTGGLLGGCEAKAFAPGQWEMAWDWITDT"
     gene            complement(434..2077)
                     /locus_tag="P5573_03085"
     CDS             complement(434..2077)
                     /locus_tag="P5573_03085"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HRT27081.1"
                     /translation="MAGKVYQPGESYKYPLIIKKLLLTPIIYSPNKEIVYRDKARFTY
                     RTLYERINRLASGLEKLGVKQGDTVCVFDYDSNRYLECYFAVPMMGAVLHTMNWRLSP
                     EQILYTMNHAEDDVVLINSDFLPVLEEIWDKLTTVKKVVLLTDEDKKPATKLNIDIEY
                     EEMLRGASPTYDFPDLDENTKATTFYTTATTGLPKGVYFSHRQLVLHTMCTALMVGSY
                     ESIGRFRSRDVYMPLTPMFHAHAWGIPYVATLLAVKQVYPGKYEPEVLLKLILGEKVT
                     FSHCVPTIIHMLVTSPVAKKVDLSHWKVIIGGAALPKGLAKAAMDLGIEIYAGFGMSE
                     TCPVVTIANLKEHMLDWDADKKVDVLIKTGLPLPLAELKVVDSQGNELPHDGKSTGEL
                     VMRVPYLTESYFKDPEKTKELWRDGWLHGGDVAYIDEEGYVQITDRMKDVIKTGGEWI
                     SSLDLESLTSQFAGVSEAAAIGIPDEKWGERPMMVVVLKPEFKGKVSADDLKQFLKKF
                     ADEGRLPKYGIPDKYVFVDAIPKTSVGKINKIELRKMYI"
     gene            complement(2265..2672)
                     /locus_tag="P5573_03090"
     CDS             complement(2265..2672)
                     /locus_tag="P5573_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27082.1"
                     /translation="MKGDLFLHEAQVGFKDATSNIQREADAVPKQHSGNAIGVPAPSP
                     ASAHEKNTVRQEKAVLPESLQEPIVISCGVAPAKIEPSPNINALSRMQYAGAVKVAKE
                     AVCHMAGAAVTEWMRVSESRRAPFRDYHTPGPL"
     gene            3119..3808
                     /locus_tag="P5573_03095"
     CDS             3119..3808
                     /locus_tag="P5573_03095"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HRT27083.1"
                     /translation="MDYRLVKSARRKKTISLQVRSGGTVVVRAPLRTSQEEVESFLEE
                     KKAWLRKMVLEQKERERTCRGKTFKTGETFLFLGKSYQLQVIKKSDGLDGNGAPLTFS
                     GGRFILESGEAVKGKTHFESWYRQKAFDHIMQRSQALSLDLQCFPSRVRLSDAHRRWG
                     SCSADDRLSINWRLIMAPPKVIDYVILHELLHIKEKSHSRRFWKLMEILMPDYREHRY
                     WLKENGHRLIV"
     gene            4007..4858
                     /locus_tag="P5573_03100"
     CDS             4007..4858
                     /locus_tag="P5573_03100"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HRT27084.1"
                     /translation="MKIEKIGLSHRPIISRRFAALGTCISDFTFANVFLFRDVYNYSL
                     VENDYTVIDYRMDNDRPCWMPLDDLRTIPREGLIRLLAKHGVLFPVPEEWLDAFQGPE
                     FSLSYKAGEQDYLYRVEKMATFRGRRLNRKRNRLKQFLSLYRHEAGELTPDRLPDAIG
                     ILDAWQAETGLEEEETDYRPCLEALHLSEPLHLDGRVYYVEGEPAGFLVGESLNHSTY
                     AIHFAKGIKSYKGLYEFMFNDAAKRLLNRYEFLNLEEDLGRESLRVAKSAYDPDLLVK
                     KFRVSLK"
     gene            4869..>5934
                     /locus_tag="P5573_03105"
     CDS             4869..>5934
                     /locus_tag="P5573_03105"
                     /inference="COORDINATES: protein motif:HMM:NF014474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HRT27085.1"
                     /translation="MDRTAKDLVALPSLNEGEEGIIQEITGGHVLASRLAGLGIVPGA
                     RVKLLRGSGGPVIVEVAGTRVALGAGEASKIMLCRVEPVPVEEPAECKTLLVALAGQP
                     NVGKSTVFNILTGLSQHVGNWPGKTVEKKEGTHTCENVELRIVDLPGTYSLTAFSEEE
                     RVARDFIINVTPDVIVLLVNAAALERSLYLLSELLLLGPPVIVAVNMIDVAEAQGIRV
                     DVQALQKALGIPVVPMVATKNRGIRDLLDSIIALSRGEIAYNPRIPNVSADHQAILAA
                     LTDLIADYVKAPYTVRWTATKLMEGDPEISQMIEKLVPAPVWSEIQRILVKHEDALHA
                     VVGGRYDWVEEVTRAAISRPR"
BASE COUNT         1337 a         1692 c         1604 g         1301 t
ORIGIN      
        1 ctggtacgcg cacccggtgg ccttcgccgc cgccagcacg gcaaggcgcg cctcttgttc
       61 gccatggaaa agggcgtgaa aggattggaa gacgactctc cctgggagcc gggacggttt
      121 gcggcgaggc atggcggaga ggtcgagaag ctggccgtcg ccggagaagg gcaatggggg
      181 gaatgggcgc aaagaacggg agggcttctc ggaggctgtg aggcaaaggc ctttgcgccg
      241 gggcaatggg agatggcatg ggactggatc accgacacat agaaccgact gccgcgttcg
      301 gcacacttga cccctttacc cactttgcca acgatcaact aaaatcaccc gatcattgac
      361 ggtatcgaag aagagggggt cgtcccctca ccggcgtgag gggacgagtt tgctatttta
      421 gtgaatccgt gagtcagatg tacatcttcc ttagctcgat tttattaatc ttgccgacac
      481 tcgtcttggg gatcgcatcg acaaacacat acttgtctgg gatgccgtat ttcggcaacc
      541 taccctcatc ggcaaacttt ttcaagaact gcttcaggtc gtcggcactc accttaccct
      601 tgaattccgg cttcaggacg acgaccatca tcggcctttc gccccacttt tcgtcgggta
      661 tgccgatcgc cgccgcctcc gaaacacccg cgaactggct ggtcaggctt tccaggtcga
      721 gagaagaaat ccattcgccg ccggttttga tcacgtcttt catcctgtcg gtaatctgta
      781 cgtatccttc ttcatcgatg tacgcgacat ctccaccgtg cagccatccg tctctccata
      841 actccttcgt cttctccggg tccttgaaat agctttccgt aaggtacggc accctcatca
      901 ccagttcccc tgtcgacttg ccgtcatgtg gcaattcatt accttgcgaa tccactactt
      961 tcaactccgc gaggggcagg gggagccctg tctttatgag tacatccacc tttttgtcgg
     1021 catcccagtc gagcatatgt tctttgaggt ttgctatggt cacgacgggg caggtctccg
     1081 acatcccaaa accggcgtag atttctatgc cgagatccat cgcggccttg gccagaccct
     1141 tcggaagcgc cgccccacct atgatgacct tccagtggga caaatcgacc ttctttgcca
     1201 ccggactcgt cacaagcata tggatgatcg tcgggacaca atgggaaaag gtcacctttt
     1261 ctccaaggat taatttcaat aaaacttcag gttcatactt gccgggatag acctgcttga
     1321 cggccagaag cgtcgctacg taaggaatgc cccaggcatg ggcgtgaaac atcggcgtga
     1381 gaggcatata aacatcgcgt gacctgaatc tcccgataga ctcataactc ccaaccatca
     1441 gagccgtaca catagtgtgg agcacaagct gtcgatgcga aaaatatacg cccttgggaa
     1501 gacctgtggt tgcagtggta tagaaggtcg tcgccttggt gttttcgtct aaatcgggaa
     1561 agtcgtatgt cggtgaagcc ccccgcaaca tctcctcata ttcaatgtca atgttcagtt
     1621 tggtcgccgg ctttttatct tcgtcggtga ggagcacgac ctttttcacc gtcgtcagct
     1681 tatcccatat ctcctctaat acggggagga aatcggaatt gatcaagaca acgtcatcct
     1741 ctgcatggtt catcgtgtag agtatctgct ccggagacaa cctccagttc atggtgtgca
     1801 gcaccgcccc catcatggga acggcgaagt aacattcaag gtaacggtta ctgtcataat
     1861 cgaacacgca gacggtgtcg ccttgcttta cccccaattt ttcgagcccg cttgccagtc
     1921 tgttgatccg ctcatacaac gtcctgtagg tgaacctcgc cttgtctctg taaacaatct
     1981 ccttgttggg cgagtaaatg atgggcgtga ggagcaattt tttgatgatc aaaggatact
     2041 tgtaactttc cccgggttga tacactttac ctgccatgac ttatccctcc ctttttttgt
     2101 ttggcgaaca gaattactgg gcgccacgct caatcatgag tgaaaaactc cacctcgacg
     2161 gacatatccg ccaggcatat tcctccttga tcagggtgta tggcacaaat cactatcccg
     2221 agactctttc tcatcgcgaa cacccctttc atgaatacga ctgttcaaag tgggccgggc
     2281 gtgtgatagt cccggaaagg cgccctccgg gattcggaca cgcgcatcca ctccgtgacg
     2341 gcagcgccgg ccatgtgaca aacggcctct ttggcgacct tgacggcccc ggcatattgc
     2401 attcggctca aagcgttgat gtttggtgat ggttcgattt tcgctggggc aaccccacaa
     2461 gagatcacga tcggttcttg aagagattcg ggaaggacgg ccttttcctg tctcacggtg
     2521 tttttctcgt gcgcggaggc gggggagggt gccggaactc cgatcgcgtt ccctgaatgt
     2581 tgcttgggca cggcgtccgc ctctcgttgg atgttacttg tggcgtcttt gaacccaacc
     2641 tgagcttcgt ggagaaagag atcccctttc accgcctgtc gggtagtccg gtcataagtc
     2701 acgactcccc gagcaaaccc tcttcccggt cgaccttgtc ggtaaaaact ctcttggggt
     2761 atcttttccc ctgccgggcc atagcttgtt gacgatggag tcgcctttcg acaagtgctc
     2821 cttccgctcc tcccgtcgca acccgatttt accgtgtcga cagtatttgg cattttaaag
     2881 gaaaaaaagc gctcggtcaa caagacaggt ggtgcatgtc aagacagatg gtgcgtctaa
     2941 gattcctatt gacactctga ttgtttcctc ataagttcaa gctcgacgga acacctttca
     3001 caccaaccca caatgaaaca ggccgcaatg aaacaggttt tttgttcgtc gcacagaagc
     3061 gctgtgaata cgcctggtca ttgttacaga tgaatcaacg cacccgacgg acgccacgat
     3121 ggattatcgg cttgtgaaga gcgcaaggag aaaaaaaacg atctctctcc aggttagaag
     3181 tggcggcacg gtcgtcgtcc gggcacccct tcgcacgtcg caagaggagg tagaatcatt
     3241 cctcgaagaa aagaaggcct ggctgcgaaa aatggtcctg gagcagaagg aaagagaacg
     3301 gacctgcagg ggaaaaacct tcaagacggg cgagaccttc ctcttcctcg gcaaatccta
     3361 ccagcttcag gtcatcaaaa aaagcgacgg gctcgacgga aacggcgccc cgctgacatt
     3421 ttccgggggg cgttttatcc tggaaagcgg cgaggccgtg aagggaaaaa cccatttcga
     3481 aagctggtac cgacagaagg ccttcgacca catcatgcaa aggtcgcagg cgctcagcct
     3541 ggacctgcag tgtttcccct cccgtgtccg gctcagcgac gcgcaccgcc gctgggggtc
     3601 ctgctctgcc gacgaccgcc tctccataaa ctggcggctg atcatggctc cgccgaaagt
     3661 catcgactac gtcatcctgc acgaactcct tcacatcaaa gaaaaaagtc attcccgtcg
     3721 cttctggaag ctcatggaaa tcctcatgcc ggattaccgg gaacaccggt actggctcaa
     3781 agaaaacggc catcgactga tcgtataagg caaaaaataa ggcaataaaa tccttgactg
     3841 gaggcgaagg tttcgcgtat cttccacaaa tctttgcagg tccgttcgct gattcagtct
     3901 cacaaaggcc ctgaagggtt atcaaagatg cacggccaac cccgcttccg acagtcagag
     3961 cggggttttt cgtgtgatat actttgcttt atcgggtaca gtctgcatga aaatcgaaaa
     4021 aataggcctt tcccatcgcc ccatcataag ccgccgcttc gccgcgctgg ggacgtgcat
     4081 ttccgatttt acctttgcca acgtcttctt gttccgggat gtctataact acagtctcgt
     4141 cgagaacgac tacaccgtca tcgactaccg gatggataac gacaggccct gctggatgcc
     4201 gctcgacgac ctcaggacca tccccagaga gggcctaatc cggctcctgg cgaagcatgg
     4261 cgtcctcttc ccggtgccgg aagagtggct cgatgccttt cagggtcccg agttttccct
     4321 ttcatacaaa gcgggcgagc aggattatct ctacagggtc gaaaagatgg ccaccttccg
     4381 gggtaggagg ctgaaccgga aaagaaaccg cttgaagcaa ttcctctccc tttaccgaca
     4441 cgaggctgga gagctcaccc ctgacaggct tccggatgcc atcggaatcc tcgatgcctg
     4501 gcaggcggaa acaggccttg aagaggagga aacggattac cggccctgcc tcgaagcgct
     4561 gcacctgtcg gaacctctgc atctcgacgg gagggtctat tacgtggagg gggagccggc
     4621 cgggttcctt gtcggggaga gtttgaacca ctcaacatac gcgatccact tcgcaaaagg
     4681 gataaagtcg tacaagggcc tctatgaatt catgttcaac gacgcggcga aacgcctgct
     4741 gaaccggtat gagttcctca acctcgaaga ggacctggga agagagtccc tacgggtcgc
     4801 caagtcggcc tacgaccccg accttctggt gaagaaattc cgggtgagtc tgaaatagga
     4861 ccagccgtat ggatagaaca gctaaagacc tggtagcact cccgtctctc aacgaggggg
     4921 aagagggtat catccaggag atcacgggcg gccacgtctt ggcgagtcgc cttgccgggt
     4981 tgggtatcgt acccggcgcc agggtcaaac tgctccgcgg cagcggcggt cccgtcatcg
     5041 tcgaggtggc cgggacgcgg gttgcgctgg gcgcggggga ggcgtccaag atcatgttgt
     5101 gcagggtcga accggtgccg gtcgaagaac cggcagagtg caagaccctg ctggtggcgc
     5161 tggctggcca gcccaacgtc ggcaaatcca cggtcttcaa catcctgacc ggactctccc
     5221 agcacgtggg aaactggccc gggaaaactg tggagaaaaa agaagggaca cacacctgcg
     5281 aaaacgtaga gttgcggatc gtggacctgc ccggcaccta cagcctgacg gccttttccg
     5341 aagaggaacg ggttgccaga gattttatca tcaacgtgac gcccgacgtg atcgtccttc
     5401 tcgtcaacgc agcggccctg gagaggagcc tctatctcct gtcggaactg cttctcctcg
     5461 gcccgccggt catcgtggca gtcaacatga tcgatgtcgc tgaagcacag gggatacgtg
     5521 tcgacgtcca ggccctgcag aaagcattgg gcatccccgt agttcccatg gttgccacta
     5581 agaacagagg tatccgggac ctcttagact cgataatcgc cctctcgagg ggggagattg
     5641 cttacaaccc ccgtatccct aacgtctccg ccgaccacca ggccatcctc gccgccctga
     5701 cggacctgat cgccgactac gtcaaggcgc cttacacagt gcgctggacg gccaccaaac
     5761 tgatggaggg cgaccccgag atctcgcaga tgatagagaa actggtgccg gctccggtct
     5821 ggagcgagat ccagcggata ctggtcaagc acgaagacgc cctccacgcc gtcgtgggcg
     5881 gccggtacga ctgggtcgaa gaagtgaccc gggcggccat ttcccggccg agac
//
LOCUS       DAOTMX010000073         5926 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_11440_length_5926_cov_4.018396, whole genome shotgun sequence.
ACCESSION   DAOTMX010000073 DAOTMX010000000
VERSION     DAOTMX010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5926
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11440_length_5926_cov_4.018396"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /gene="trpD"
                     /locus_tag="P5573_03110"
     CDS             complement(<1..206)
                     /gene="trpD"
                     /locus_tag="P5573_03110"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417724.1"
                     /note="Catalyzes the conversion of
                     N-(5-phospho-D-ribosyl)-anthranilate and diphosphate to
                     anthranilate and 5-phospho-alpha-D-ribose 1-diphosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HRT27086.1"
                     /translation="MIREALAEVVNGRNLKEADMIEVMDEVMEGKATPAQIAAFITAL
                     RVKGETVDEVTGAARVMRQKATRI"
     gene            complement(260..850)
                     /locus_tag="P5573_03115"
     CDS             complement(260..850)
                     /locus_tag="P5573_03115"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HRT27087.1"
                     /translation="MILMIDNYDSFTYNLVQYLGQLGADVQVRRNDETTLEEVERLGP
                     RAIFISPGPCSPKEAGLAVDIVRHFYRTIPIMGVCLGHQAIGCAFGGEVVRAPRVMHG
                     KTSPVISDGRTIFKGLPNPFTAGRYHSLIVHRPTLPSCLEVSAETAEGEIMGIRHVEY
                     PVEGVQFHPESILTPQGKRIVKNFLELAGINGNGKN"
     gene            complement(847..2340)
                     /gene="trpE"
                     /locus_tag="P5573_03120"
     CDS             complement(847..2340)
                     /gene="trpE"
                     /locus_tag="P5573_03120"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HRT27088.1"
                     /translation="MYYPTFAEFEEMANRGNLIPVYREILADTETPVSALMKLRRKKR
                     LFLLESGEGGEKWGRYTFLGADPKTVYEVRGNEVVIEENGRTRRFDHGGDPLGYLEKL
                     IGDCRPISPEGLPRFYGGAVGFLGYEMVRFFERLPERDRHSGTADAAFLITDTLMVFD
                     NVRHTIKAVACVHTEGTKDLRSLYDDAVRRIEAMVDTLRGSVLDEKAYPEGTNEEPFA
                     ANMTPEEFKSMVRKAREYILAGDFIQVVVSQRFEGRNRLDPVNLYRALRFVNPSPYLF
                     FLKLDDVTLIGSSPEVMVRLEEGIAELKPIAGTRPRGKSEQEDRMLSDELLRDPKERA
                     EHVMLVDLGRNDLGRIARVGSVQVDQLMVVERYSHVMHLVSHIQAQLADGMDCFDVLK
                     ATFPAGTLTGAPKVRAMEIIDELEPTARGPYGGAVGYFSFTGNMDFCITIRTMMLKDG
                     KIYIQAGAGVVADSDPDAEYRETVNKAEGMRQAINLAASGFELRRIK"
     gene            2853..3095
                     /locus_tag="P5573_03125"
     CDS             2853..3095
                     /locus_tag="P5573_03125"
                     /inference="COORDINATES: protein motif:HMM:NF017786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapB"
                     /protein_id="HRT27089.1"
                     /translation="MELVKFSQLEEKIKGLIDQHALLKREKENLEGLLKKKEGELEEA
                     KDTLKKLNEERDAIRAKVDSLLDMLHDIGEPQLPLR"
     gene            3100..3378
                     /locus_tag="P5573_03130"
     CDS             3100..3378
                     /locus_tag="P5573_03130"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HRT27090.1"
                     /translation="MKKRFSIRILGQELSVLSDARDDHVAAVERYVNEKIEEVKKTTA
                     DQTTLNIIMLAALNIADEYLRLKGEKEDFYKELETKSEKLINFIEERQ"
     gene            3388..3576
                     /gene="ssrS"
                     /locus_tag="P5573_03135"
     ncRNA           3388..3576
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="P5573_03135"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            3700..5265
                     /gene="rny"
                     /locus_tag="P5573_03140"
     CDS             3700..5265
                     /gene="rny"
                     /locus_tag="P5573_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HRT27091.1"
                     /translation="MENLFYDIVLVCLSLAVGVVIGYFVRKKLSQKQLESAEALSARI
                     IEEARKESENIKKESILQAKDYLLKAKAEFEKDTQERKSDIEALERRLRSKEENLEKR
                     LDSLAQKEGNIESREKELLQKEALIAEKHTKMDQMLEEQRKTLEKIAGISAEEAKNYL
                     IRSMEAEARRESAAALRRIEEETKREGEKKAREILAYAMQRYAGDFVVENSVSVVNLP
                     NEEMKGRIIGREGRNIRAIEAATGIDLIIDDTPEAVVLSSFDPVRREIAKMALERLIS
                     DGRIHPGRVEDIVKKVKVEVENIIRETGERVSFDVGVHDLHPELVNILGKLKFRTSFS
                     QNVLQHSIEVAHLTAIMATELGMNVKEAKRAGLLHDIGKALDHKMEGTHAAIGASVAK
                     RFGENERIVQAIATHHDDGRTNTLLGVLVQTADTLSAARPGARREMLETYVKRLEELE
                     KIANSFTGVDKSYAIQAGREIRILVESEKVSDDDALLLCKDIAKKIESELTYPGQIKV
                     TVIRETRVSELAK"
     gene            5271..>5926
                     /locus_tag="P5573_03145"
     CDS             5271..>5926
                     /locus_tag="P5573_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HRT27092.1"
                     /translation="MKILFVGDIVGKPGRRVLRELMPGVVNRHGIDLTIANGENAAGG
                     FGITREVVDELFENNVQVLTTGNHVWDKKESEGFIDDYVNLLRPANYPPGVPGRGFTI
                     VADGLGNKVGVINLMGRVFMRPIDCPFRTAEAAIKKISAVAKVIVVDIHAEATSEKQA
                     VGWFLDGKVSAVIGTHTHVQTSDNRVLTNGTAYITDAGMTGPLDSVIGVQKEKVLGSF
                     L"
BASE COUNT         1430 a         1530 c         1670 g         1296 t
ORIGIN      
        1 tcgatcctcg tcgctttctg tctcatgacg cgcgccgccc cggtgacctc gtcgacggtc
       61 tcacctttga cccggagcgc cgtgatgaag gccgcaatct gggccggcgt cgccttgcct
      121 tccatcacct cgtccatgac ttctatcata tccgcttcct tcaggtttcg tccgttgacg
      181 acctctgcca atgcttccct tatcatcttc ccctcctcgg gattggtatt ctttgctggg
      241 ccatgctttc ttcttagtct caatttttcc cgtttccgtt gatacccgcc agttcgagaa
      301 aattcttgac gatccgcttt ccctgcggcg tgaggatcga ttccgggtgg aactggaccc
      361 cttcgaccgg gtactcgacg tgtcttatcc ccatgatttc gccttccgcc gtctcggcgc
      421 tgacctcgag acaggaagga agggtcggcc ggtggacaat caaggaatgg taccgccccg
      481 cggtgaaggg gttgggaaga cccttgaaga tggtacggcc gtcgctgatc acgggcgacg
      541 tcttaccgtg catgaccctc ggggcccgca caacctcccc gccgaaggca caaccgatgg
      601 cctgatgacc gaggcagacg cccatgatgg ggatcgtcct gtaaaaatgt cttactatgt
      661 cgaccgcgag tcccgcctcc ttcggcgaac aggggccggg ggagatgaaa atcgcccggg
      721 gccccagccg ttcgacctct tcgagggtcg tttcgtcatt acggcggacc tggacgtcgg
      781 cgccgagctg tcccaggtac tggacgagat tgtaagtaaa ggaatcataa ttgtcgatca
      841 tcagtatcat tttatcctcc ttagttcaaa acccgaggcc gccaggttga ttgcctgcct
      901 catcccctcg gccttgttga cggtttctct gtattccgcg tcggggtcgg agtcggccac
      961 gacacccgcg ccggcctgga tgtatatctt gccgtctttg agcatcatcg tccggatggt
     1021 gatgcagaag tccatgttgc cggtaaagct gaaataaccg accgccccgc cgtaaggacc
     1081 cctcgccgtc ggttccagct cgtcgatgat ctccatggcc cttaccttgg gtgcgcccgt
     1141 cagggtaccg gcggggaaag tggctttgag gacgtcgaag cagtccatac cgtcggcaag
     1201 ctgggcttgg atgtgagaca ccaggtgcat gacgtgggaa tacctttcca caaccatcaa
     1261 ctggtcgacc tggacgctcc cgacccgggc gatcctcccc aggtcgttgc ggccgaggtc
     1321 cacgagcatc acatgctccg ccctttcctt cgggtcccgg aggagctcgt cggagagcat
     1381 cctgtcttcc tgctcactct ttcccctcgg cctggtcccg gcgatcggct ttagttccgc
     1441 tatcccttct tcgaggcgga ccatcacctc gggcgacgac ccgatgaggg tcacatcgtc
     1501 gagttttagg aaaaagaggt acggtgaggg attaacgaac cggagggccc ggtacagatt
     1561 gaccgggtcc aacctgtttc tgccctcgaa gcgctgggag acgacgacct ggatgaaatc
     1621 gccggcaagg atgtattccc gggccttcct caccatggac ttaaactcct cgggagtcat
     1681 attggccgcg aaaggctcct cgttggtccc ctcagggtac gctttttcgt caaggacaga
     1741 cccgcggagc gtgtcgacca tcgcctcgat tctccgcacc gcgtcgtcgt acaggctccg
     1801 aaggtcctta gttccctctg tgtggacgca ggcgacggcc ttgatcgtgt ggcggacgtt
     1861 gtcgaacacc atcagcgtgt cggtgatcag gaaggcggcg tcggcagttc cactgtgacg
     1921 gtcgcgttcc ggtaagcgct cgaaaaacct aaccatctca tagccgagga atccgactgc
     1981 cccgccgtag aaccgcggaa ggccttccgg cgaaatgggg cgacagtcgc cgatgagctt
     2041 ctcaaggtat cccaggggat cgcccccgtg gtcgaaccgg cgggttcggc cgttttcctc
     2101 gatcaccacc tcattacccc ggacctcata gacggtcttc gggtcggcgc cgaggaaggt
     2161 gtagcgaccc catttttcac cgccttcacc gctttccagc aaaaagaggc gttttttccg
     2221 ccgtagcttc atgagggccg acacgggcgt ctccgtgtcg gctaggatct cccggtagac
     2281 ggggataagg tttcccctgt ttgccatctc ttcaaactct gcgaatgtcg gatagtacat
     2341 attgcttgcc ccaaaaaaag gccgtgaagc ttctgctcac ggccccggtt tttctgtgct
     2401 caaaaaacga aagggccgtg aagtcccgag aggttctcca cggcccgttg tcggttaagt
     2461 gctacgtttc agcggtggac aaacccctcc cctggaatct gccaccacca gttaatcatt
     2521 gctgtctttt taacctgcat attatgcttt ctatctaaga aatgtgcaga ttcctataac
     2581 agggttcatc ggagttgtca actcaatttt gacgccctcg taaaaagtcc aaaaacgtca
     2641 tgccggaccc gacccggcat ccagaacata ttgaatatac tggattccgg cgttcgccgg
     2701 aatgacgaaa gtggtaattc tcgccttttt acgggattgt caattttctc tctgcaaata
     2761 aagcgcttga cttttttagg ggggttgaaa taagttgtca ataaatccta cagttgaaaa
     2821 cagagcaaag agaaaggtaa atcggtgctt tcgtggagct cgtgaaattc agtcaacttg
     2881 aagaaaagat caaagggttg atagaccaac atgctttact gaaaagagag aaagagaatc
     2941 tggaggggtt acttaaaaaa aaggaagggg aattagaaga ggctaaggac acgctgaaaa
     3001 aattgaacga ggaaagggac gcgatacgag caaaggtgga ttcactgctt gacatgctcc
     3061 atgatattgg tgagccgcaa ttgcctctga ggtaaaacct tgaaaaagcg cttcagcatc
     3121 aggattctcg gacaggaact atccgtcctg agcgatgcac gggatgatca cgtggcggca
     3181 gtagagcggt atgttaacga aaaaatagaa gaagttaaaa agactacggc ggatcagacg
     3241 acattgaaca tcatcatgct tgccgcttta aacatcgccg atgagtatct aaggctgaag
     3301 ggtgaaaaag aagattttta caaggagttg gagaccaagt cggaaaaatt gataaatttt
     3361 atcgaagaaa gacagtaatt ggtacggtat cccctgcgat gttcgtgatt aagatgagtc
     3421 tttgagccaa cacattgaag cccgggggcc tttaattgcg tgtggtgtgc atgtctgaat
     3481 ctccctcgga gatccagaaa gcctgaagcc gcaacagggc gcccaccttt tataaaggtt
     3541 caaagggttt attaacacgg cataagcggg ggacttaatt ttgtcccccc tgaaggcccc
     3601 ttatcaggat tcgggggcaa gcgcttttaa gttttccctc cgtaagcccc ttccaggcaa
     3661 tcgttcttat acccctttgt ccaatgtata catgaggagg tggaaaattt gttttacgat
     3721 atagtgttag tctgcttatc gctcgccgtg ggcgtcgtca taggttattt cgttcgcaag
     3781 aaactttcac aaaaacaact ggagtctgcg gaagcccttt ccgcccgaat aatcgaggaa
     3841 gcgagaaaag aaagcgaaaa catcaagaag gagtcgatcc tccaggcaaa ggactatctt
     3901 ctcaaggcaa aggcggagtt cgaaaaagat acacaggaga gaaagtccga tatagaagcc
     3961 ctggaaagaa ggctgcgttc caaggaagag aatttagaga aaaggctaga cagcctggct
     4021 cagaaggaag gtaacatcga gagccgggaa aaggagcttt tacagaaaga agcccttata
     4081 gcggaaaagc acaccaagat ggaccagatg ctcgaagagc agaggaaaac cctcgagaag
     4141 atcgccggca tctccgcgga ggaggcaaag aactatctca tccggtctat ggaggcggaa
     4201 gccagacggg aatcagccgc tgctttgcgg aggatcgaag aagaaacgaa gcgggaggga
     4261 gagaaaaaag cacgtgaaat cctggcttac gccatgcaac gttatgccgg tgatttcgtg
     4321 gtggaaaatt ctgtttccgt tgttaatctt ccgaatgaag aaatgaaggg aaggatcatc
     4381 ggccgggaag gaaggaatat ccgggccatc gaagcggcta cgggcataga cctgatcatc
     4441 gatgatacgc ccgaggcggt cgttctttcc agtttcgacc cggttcgcag ggaaatcgct
     4501 aagatggccc tggagcggtt gatcagcgac gggaggatcc atcccggcag ggtggaagac
     4561 atagtcaaga aggtgaaggt tgaggtcgaa aacatcatcc gggaaacggg ggagagggtt
     4621 tctttcgatg tgggcgtcca cgacttacat cctgagctgg tcaacatcct gggcaagctc
     4681 aaattccgca ctagcttttc ccagaacgtc cttcaacatt ccatcgaggt ggcccatctg
     4741 acggccatca tggctacgga actggggatg aacgtgaagg aggccaaaag ggcgggcctc
     4801 ttgcacgaca tcggaaaggc cctcgaccac aagatggaag gcacccatgc cgccatcggt
     4861 gcaagcgtcg caaagcgctt cggtgaaaat gaaagaatcg tgcaagcgat tgcgacccac
     4921 cacgacgacg ggcggaccaa caccttgctt ggggttttgg tccagacagc ggacacgctc
     4981 tcggcggcga gaccgggggc gcgccgcgag atgctcgaaa cctacgtcaa acgcctcgaa
     5041 gaactggaaa agatagcgaa ttcctttacg ggtgtcgaca agagttatgc catccaggcc
     5101 ggccgggaga ttcgtatcct cgttgaaagc gaaaaggtgt cggacgatga cgccctgctt
     5161 ctctgtaagg acatcgccaa gaaaatagag tcggaactga cataccccgg acagatcaag
     5221 gtcacagtga tcagggaaac cagggtatcg gagttagcaa aatagaagac atgaaaattc
     5281 tttttgtcgg tgacattgtc ggaaagccgg gcaggagggt cctcagagag ttgatgccgg
     5341 gggtcgtgaa tcgacacggg atcgacctga cgattgctaa cggtgaaaac gccgcaggag
     5401 gcttcggcat aacccgggag gtggtggatg agcttttcga gaacaacgta caggtcctga
     5461 ctacgggcaa tcatgtgtgg gataagaagg aatctgaagg ttttatcgac gattacgtga
     5521 accttctgag accggctaat tatcctcccg gtgtacccgg ccggggattc accatcgtcg
     5581 cggacgggtt gggtaacaaa gtgggcgtga tcaatctgat gggacgggtt ttcatgcgac
     5641 ccatcgattg tcccttccgt acggccgagg cggcaatcaa gaaaatcagc gcagtggcaa
     5701 aagtgattgt tgtggatatc catgctgagg ctacatcgga gaaacaggct gtgggctggt
     5761 ttcttgacgg caaagtaagc gctgtcatcg ggacgcatac ccacgttcaa acctccgaca
     5821 atagggtgct tacgaatggt acggcctata tcaccgatgc cgggatgacg ggtcctctgg
     5881 actctgtcat cggggtccag aaggaaaagg tgctggggag cttcct
//
LOCUS       DAOTMX010000074         5912 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_11479_length_5912_cov_3.321666, whole genome shotgun sequence.
ACCESSION   DAOTMX010000074 DAOTMX010000000
VERSION     DAOTMX010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5912
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11479_length_5912_cov_3.321666"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..709)
                     /locus_tag="P5573_03150"
     CDS             complement(<1..709)
                     /locus_tag="P5573_03150"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HRT27093.1"
                     /translation="MKARRDVDDGRSRARGGVWLLTFNDMMTLLLTFFVLLLSLSKLD
                     VAKAKAASDAVGAAFGVPGVAEELSVRVFDPFVFSPGPFEFHGTQAGQDAALRDGAGR
                     FIEKRDELIRRFKGGGALSVEASPKGVIISADGAALFKAGLAEPAAQMQAILAPVCPL
                     LEEAGLLAKVEVYTDEVPADRQYFPSCWELAASRGAVVADYLASHGAEPDRVAVSGYG
                     IAKPAKTGQRGRGTSAGS"
     gene            complement(744..1412)
                     /locus_tag="P5573_03155"
     CDS             complement(744..1412)
                     /locus_tag="P5573_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27094.1"
                     /translation="MLRRHNEIAEDRRGESWQMTYCSTIAILVAVFLMVLSYSNFEGK
                     KVKGFVGRVEGKRLPGYATSGEGGPANPAEIKIEAAMDDVRRAIALRGKGDQYTMVRT
                     KKGFKVTFPADALFDAGTLKIRDDARPLLDDLASAVADSPFFLGIVSHVARATATENI
                     SRLSWEVTAARAGVIMRHLSERGGVPLKRLAAAGFSHHSRAAAVSQEISGRDERVELC
                     FELL"
     gene            complement(1412..2173)
                     /locus_tag="P5573_03160"
     CDS             complement(1412..2173)
                     /locus_tag="P5573_03160"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="motility protein A"
                     /protein_id="HRT27095.1"
                     /translation="MDLATILGIATGFGLVILAMATGGGLSWFLDAPSAMIVGGGLVG
                     ALLISYPLPTVLRVMAVAKNVFFQKVQPPEQVIRKIVEMSRVSRKEGILALQRMVPGL
                     KDPFFVKAVELMMDGMEPEKLTDILETELESVAERHRLGAEIFTSLGNFAPAMGMTGT
                     LIGLVKMLMQMNDPSSIGPSMSVAFVSTFYGVVAANLIFFPIAGKLRMLSAQELLVKQ
                     LIIGGIVSVLEGDNPRVVEQKLHSFISPEKRRSVF"
     gene            complement(2442..3992)
                     /locus_tag="P5573_03165"
     CDS             complement(2442..3992)
                     /locus_tag="P5573_03165"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HRT27096.1"
                     /translation="MGSALWAGISGLNASSKELDVIANNIANVNTVGFKASKTYFGDV
                     LSQSISGGSSGNMQVGRGVMVTDIATQFGAGSFETTGNATDVSIDGDGFFMVNDKNGA
                     TYYTRAGAFHMDNEGYLVDVNGYRVQGYNLTGSSSTTIDDIALANVQSAPKVTSTFSF
                     GLNLNAEEPAGGTFNCSQTVYDSLGAKHTLNVAYTKTEENGCWGFQVSLDGVEAAAQT
                     YSGMKFDAAGNLEKLYTASVEGLTITGAGTAEATVNKPGQLYKSTVIVNEEGNEEVVK
                     IKLTRGEGATSWTIDENGGYDNMSVTATADTVEIDLDGAGGADITFALAGAWAENNTI
                     EFAITCTEVDPVDLAVTFGNISNGATIGNSNVVTWDIEGSSAPPITGYASTSVIRSLS
                     NDGYASGILRSLSIDGEGKISGFFTNGQTSNIAQLVLADFENPWGLKKMGSNLFGETI
                     TSGPAIKNVPGASGMGEVKSNCLEMSNVDIATEFINMITAQKAYQANARVITTQDQVL
                     TELMNIKR"
     gene            complement(4044..4658)
                     /locus_tag="P5573_03170"
     CDS             complement(4044..4658)
                     /locus_tag="P5573_03170"
                     /inference="COORDINATES: protein motif:HMM:NF025230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HRT27097.1"
                     /translation="MSTGSVGSATNSVMGKDVFLKMLIAQLKNQDPLNPLSGADFAAQ
                     LAQFSSVEQLINVSSQLESLNTNIAAMANSQMSNLIGCEVTFEGNTVAADGTAKTLSY
                     SLPEDVAKGTVSIYDEKGKLVKTIDFGSQQAGVNSVTWNPGSLTGTYTFQVNAQNKSG
                     STVKGTTLATGTVSGITFKNGSSYLVVGGQEVAFSTVASVRKAG"
     gene            complement(4681..>5912)
                     /locus_tag="P5573_03175"
     CDS             complement(4681..>5912)
                     /locus_tag="P5573_03175"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HRT27098.1"
                     /translation="EAAIRSIAGPTGLIENRQGVRESATEGRLSSRTMVGGQRRESSR
                     EASNTATTADRPLSKTAGRLGHLLLESVEEGGNGEIKAADAAGLYRGLGATAKADSRS
                     TGMKKDAGARTMPGLKAGDREGPADVKMGALPAGNRATSSSKVSVKAEGLGRRDEPSG
                     DTSRVSASDGTSGGKEEQAASGKVKEGDAKGFKDQIASYREQVKTIEGSGGETPSKEL
                     RLQVDAKSAPQQKSYEIQVNHGLSVEAKEPSSAAAKETPSRPVSPQVVIDRIAENGHL
                     LLRAGSGRVRLTLTPPQLGTLDVDLRVSRDRLKMVLTAENGEVRQILQSNMDQLKSTL
                     QGQGWNLERMDVVVQDQYGKDLGQPDQGSRYFGGDDPGRGRGDGGRESGSFQERYQPG
                     CDDTPFLNGDGMLSVFV"
BASE COUNT         1132 a         1933 c         1541 g         1306 t
ORIGIN      
        1 ccgagcccgc agacgttcca cgcccccttt gtcccgtctt cgccggtttt gcgattccat
       61 accccgaaac cgccacccgg tccggttcgg cgccatggga cgccagatag tcagccacga
      121 cggccccccg cgatgctgca agttcccagc acgagggaaa gtattgcctg tccgccggca
      181 cctcatccgt gtacacctcg accttcgcga gcagccccgc ttcctccaac agagggcaga
      241 ccggggccag aatcgcctgc atctgcgccg ccggctcggc caaccccgcc ttaaaaaggg
      301 cggcgccgtc cgcgcttata attacaccct tcggggaagc ttcgaccgac agtgcccctc
      361 ctcccttgaa acgacggatg agttcgtccc ttttctcgat gaaccggccc gcaccgtccc
      421 tcaaagccgc gtcctgcccc gcctgcgttc cgtggaactc aaacggcccg ggagaaaaaa
      481 cgaaagggtc gaagacccgg acggaaagct cttccgcgac gccgggaacg ccgaaggccg
      541 caccgaccgc atcagaggcc gctttcgcct tcgccacgtc aagcttcgac aaagagagga
      601 gcagaacgaa gaatgtcagc aataacgtca tcatgtcgtt gaatgtcagc agccacacgc
      661 cgcccctggc gcgggacctt ccgtcgtcca catctcttct tgccttcatg accggccctt
      721 ttcccgacga gttgaaatcc cacctaaagg agttcgaaac ataactccac cctttcgtcg
      781 cgaccggaaa tttcctgact tacggccgct gcccggctgt ggtgcgagaa gccggccgcc
      841 gccaatcgct tcaggggaac ccctcctctt tccgacagat gcctcatgat gacccccgcc
      901 ctggccgccg tgacctccca ggacagcctg gagatatttt ccgttgccgt cgccctagcg
      961 acgtgactga cgatgcccag aaaaaaggga gagtcggcga ccgccgacgc gaggtcatct
     1021 aaaaggggcc gggcatcgtc ccggatcttc agtgtgccgg catcgaacag cgcgtccgcc
     1081 ggaaacgtga ccttgaaccc ctttttcgtc ctgaccatcg tgtattgatc tcctttaccc
     1141 ctgagggcga tagcccttct tacgtcgtcc atcgccgctt ctatcttgat ctccgccggg
     1201 ttcgccggcc cgccttcgcc ggaggtcgcg taaccgggga gccgcttccc ctcgaccctg
     1261 cccacgaacc ccttcacctt tttcccctcg aaattggaat aggacaggac catcaagaaa
     1321 acggcgacga ggatggcgat cgtcgagcaa taggtcatct gccagctctc accccttctg
     1381 tcttccgcta tttcgttatg ccgccgtagc atcagaatac cgacctcctc ttttccggcg
     1441 agatgaagga gtgcagcttt tgctccacga cccgcggatt gtcgccttcc aggacggaga
     1501 ctatcccacc gatgatcagt tgcttgacca gcagctcctg ggcgctcaac atccgcaact
     1561 ttcccgcaat ggggaagaag atgagattcg cggcgacgac gccgtagaaa gtagagacaa
     1621 aggccaccga catcgacggt ccgatgctgc tcgggtcatt catctgcatc aacatcttga
     1681 cgaggccgat cagcgtcccc gtcatcccca tggcgggcgc gaaattgccg agggacgtaa
     1741 agatctctgc gccgaggcga tgtctctcgg ccacggattc gagctccgtc tccaggatgt
     1801 cggtcaactt ttccggttcc atcccatcca tcatcagctc aacggccttc acaaaaaaag
     1861 ggtctttcag ccccggcacc atccgctgga gggcgaggat gccctctttc ctggataccc
     1921 tcgacatctc gacaatcttc ctgattacct gctcaggggg ctgcactttc tgaaaaaaga
     1981 cgttcttcgc aaccgccatc actctgagga ccgtcgggag tggatagctg atcaacagcg
     2041 cgccgacgag ccccccgccg acgatcatgg ccgagggggc gtccaaaaac caggagaggc
     2101 ccccgcccgt cgccatagcc aagattacga gaccgaatcc cgtcgcgatt cccaggatgg
     2161 tggccagatc cattcagtac gcccttctca cccggctccg ctgaaatctc taaagcacgc
     2221 agacataacc gtcgctcgaa ccgataccga gcaaataatg taccagggga aacaagagaa
     2281 aagagttgta tttctgaggc agtcctaacg agggaacagg acgtcttagg cggtcttttt
     2341 gacggggggc cgattatttg acagggcgcc tgcccttcgg tcaactcagg gagacgccct
     2401 gtccaccatt taccatattg tattcaacac ttacatggtt gctaccgttt gatgttcatc
     2461 aattccgtca atacctggtc ctgggtcgtg atcacccttg cgttcgcctg gtacgccttc
     2521 tgggcggtga tcatgttgat gaactcggtg gcgatgtcga cattcgacat ctcgaggcag
     2581 ttggatttga cctcccccat ccccgatgca ccggggacgt tcttgatcgc cggacctgac
     2641 gtgatggtct ctccgaagag gttcgatccc attttcttca gtccccaggg gttttcgaaa
     2701 tcggcgagca cgagctgggc gatgttggat gtctggccgt tggtgaagaa cccactgatc
     2761 tttccttcgc cgtcgatgga aagactcctc aggatgccgg aggcgtagcc gtcgttgctc
     2821 agtgacctga tgacggaagt gctcgcgtat ccggtgatgg gaggcgcaga cgatccctct
     2881 atgtcccagg tgaccacatt tgaattgccg atggtagcac cattggatat attaccgaag
     2941 gtaacggcga ggtcaaccgg gtcaacctcg gtacacgtga tggcaaattc aatggtgttg
     3001 ttctctgccc atgcccccgc aagagcaaag gtgatgtcgg cgccgcccgc cccgtcaagg
     3061 tcaatctcaa cggtatcggc ggtcgccgtc acggacatat tgtcgtaacc accgttctca
     3121 tcgatcgtcc aggacgttgc gccctcccct cttgtaagtt ttatcttaac tacctcctca
     3181 tttccctctt catttacaat tacagtgctt ttatagagct gacccggttt gttcaccgtg
     3241 gcctcggcgg tcccggcgcc ggtgatcgtc agcccctcga cgcttgccgt gtagagcttc
     3301 tccaggtttc cggcggcgtc gaatttcata cccgagtagg tttgtgcagc ggcttcgact
     3361 ccatcaagtg aaacctgaaa cccccagcag ccattctcct ctgtctttgt ataggccacg
     3421 ttgagggtgt gcttcgcacc cagggagtcg tacacggtct gggagcagtt gaaggtccca
     3481 cccgccggtt cttccgcatt caggttgagg ccgaaggaga aggtagacgt caccttcggt
     3541 gcagactgga cgtttgcgag agcgatatcg tcgatggtcg tcgaagacga ccctgtcagg
     3601 ttgtaaccct gcaccctgta gccgtttacg tcgacaaggt atccctcgtt gtccatatgg
     3661 aaggcccccg cccgcgtgta gtacgtcgcc ccgttcttgt cgttcaccat gaagaacccg
     3721 tcgccgtcaa tagagacgtc cgtcgcatta cccgtcgtct cgaaagagcc ggcgccgaac
     3781 tgcgttgcta tatccgtgac catgactccc cgtcccacct gcatgttccc cgacgaacca
     3841 ccggagatac tctggctcag gacgtcgccg aaataagtct tactggcctt gaaaccgacg
     3901 gtgttgacgt tggcgatgtt gttggcgatg acgtccaatt ccttagaact ggcattcagg
     3961 cccgaaatcc ccgcccacag tgcacttccc atactgtcct ccttttcgaa aaattttatt
     4021 cgatcaaatt acctatgcga ggctcatccc gcctttctga cggacgcaac ggtcgaaaac
     4081 gccacctcct gccccccgac caccagataa gaagagccgt tcttaaacgt gatgcccgac
     4141 acggtccctg tggccagcgt cgttcccttc accgtggacc cgcttttatt ctgggcgttg
     4201 acttgaaacg tgtacgtccc ggtaagggag ccggggttcc aggtgaccga gttcaccccc
     4261 gcctgctgac tcccgaaatc tatcgtcttg accagcttgc ccttctcgtc atagatcgag
     4321 acggttcctt tagccacgtc ctcggggagc gagtaagaca gggtcttcgc cgtaccgtcc
     4381 gccgcgaccg tgttcccctc gaaggtgacc tcgcagccga tcagattcga catctggctg
     4441 ttggccatcg ccgcgatatt agtgttcaga gactcgagct gactgctcac gttgatgagt
     4501 tgctcgacgc tggaaaactg cgccagttgg gcggcgaagt cggcgccgct gagcggattc
     4561 agcgggtcct ggttcttcag ttgggcgatc aacatcttca gaaagacgtc cttccccatc
     4621 accgagtttg ttgcgctgcc tacacttccc gtgctcatcc tgtcacctca ctttcatgat
     4681 ttatacaaag acactcagca tgccgtctcc gttcaggaac ggcgtatcat cacaccccgg
     4741 ctgataccgc tcttgaaaag acccggactc ccgcccgccg tctccccggc cccgtccggg
     4801 atcatctccc ccgaagtagc ggctcccttg gtccggctgc ccaaggtctt ttccgtattg
     4861 atcctgtacc acgacatcca tccgctcgag gttccacccc tgtccttgca gggtactttt
     4921 caactgatcc atgttcgatt gtaaaatctg gcggacttca ccgttttcgg ccgtcagcac
     4981 catcttgagc cggtcacggc tgacccggag gtccacgtcg agagtgccga gctggggcgg
     5041 ggtcaaggtc aaccttaccc ggccggaacc cgccctgaga aggagatgcc cgttttccgc
     5101 gatcctgtcg ataacgacct ggggtgacac cggcctggac ggtgtttcct tagcggctgc
     5161 ggaagacggt tccttagcct ctaccgaaag accatgattt acctgaatct cgtaggattt
     5221 ttgctggggg gccgactttg cgtctacctg aaggcgaagc tccttcgacg gcgtctcacc
     5281 cccggaccct tcgatcgttt ttacctgttc ccgataagat gcgatctgat ccttgaagcc
     5341 cttcgcatcg ccctctttta ctttcccgga cgcggcctgc tcctcttttc ctccagaagt
     5401 gccgtcggag gcagacaccc gggatgtgtc cccggagggt tcgtcccttc gtcccaatcc
     5461 ctccgccttg acgcttacct tgctcgagga cgtagccctg ttccccgcgg gaagggcgcc
     5521 catcttcacg tctgccggac cctcgcgatc ccccgctttc aaaccaggca tagtgcgggc
     5581 ccctgcatcc tttttcatcc cggtcgaccg tgaatccgcc tttgccgtgg cgccgaggcc
     5641 ccggtataag ccggctgcgt ccgccgcttt gatctcgccg ttcccgcctt cctcgacgga
     5701 ctcaaggagc agatgaccta aacgacccgc tgttttggaa agcgggcggt ccgccgtcgt
     5761 tgccgtgttt gacgcctccc gggaagattc ccggcgctga ccaccgacca tcgtcctgga
     5821 agacagccgc ccttcggtcg ccgactcccg cacgccctgc cgattctcta ttaagccggt
     5881 tgggcccgct atactccgga tcgcggcctc gg
//
LOCUS       DAOTMX010000075         5905 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_11489_length_5905_cov_3.523761, whole genome shotgun sequence.
ACCESSION   DAOTMX010000075 DAOTMX010000000
VERSION     DAOTMX010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5905
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11489_length_5905_cov_3.523761"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..860)
                     /gene="lsrF"
                     /locus_tag="P5573_03180"
     CDS             complement(57..860)
                     /gene="lsrF"
                     /locus_tag="P5573_03180"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: protein motif:HMM:NF006081.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HRT27099.1"
                     /translation="MLGTMDWGMKNRMSQLFKEDGRCFFLPIDHGYFQGPTSCLERPG
                     ETIAPLLPWADALFVTRGVLRSAIDPAKSKPIILRVSGGSSVIGRDLANEGITTSIEE
                     IIRLNATAVGVSIFVGSDYEYESLMNLADIVNDCEDFGIPVMAVTAVGKELQKQEARY
                     LSLSCRIAAEFGARVVKTYWCKNFDKVVEGCPVPVVMAGGPKCETELEVLEFVHDGMQ
                     RGAVGVNLGRNVWQNPQPVAMMRALHAIIHENASVKEAHKLFTSLKKST"
     gene            complement(880..1404)
                     /locus_tag="P5573_03185"
     CDS             complement(880..1404)
                     /locus_tag="P5573_03185"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HRT27100.1"
                     /translation="MKKFILIPCLIVFSVLLLSSVSASAAEKIGFINMREIIDRSDAG
                     KAIKAELEKAVEGKKAAIQQKEKELKKAKENLDKQRSVLTPQAMQEKEMQYQVEYREY
                     ERLVKDSAEELRAKDQFMANKLFPEIIKVVKAIGQREKYAAILDESAALYSSKENSLT
                     DKVIKELNKTYQGK"
     gene            complement(1544..2230)
                     /gene="queC"
                     /locus_tag="P5573_03190"
     CDS             complement(1544..2230)
                     /gene="queC"
                     /locus_tag="P5573_03190"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HRT27101.1"
                     /translation="MKRKAVVLTSGGIDSTTAMAVAREEGYELYSLTFGYGQRHAVEL
                     DAARRVAEAFGAKEHLLIRLDLRKIGGSALTADIPVPKSRTGGEMRQEIPVTYVPARN
                     TIFLSYALAWAEVLEAPDIFVGVNAIDYSGYPDCRPEYIRAFEEMANLAIKAAVEGRF
                     RVKIHAPLINLSKAQIILKGKALGVDYSLTHSCYDPSPEGKACGRCDSCLLRKKGFRE
                     AGVEDPTVYA"
     gene            complement(2240..2884)
                     /locus_tag="P5573_03195"
     CDS             complement(2240..2884)
                     /locus_tag="P5573_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRT27102.1"
                     /translation="MSLKVNEVFYSIQGESSYAGFPCVFVRLTGCNLRCSWCDTEYAR
                     EEGAAMDIGEVLERVKGYGCRLVEITGGEPLIQEETTELIRRLLDEGLAVLLETNGSM
                     DVGAVDRRCVKVVDLKCPSSGESGCNNFRNLERLTEKDEIKFVVGDRADYEYACKVLP
                     LIRERCPGSTVLFSPLSGRMEPRRLAGWILEDRLFVRLQLQLHRVLWPEKNRGV"
     gene            complement(2885..3265)
                     /gene="queD"
                     /locus_tag="P5573_03200"
     CDS             complement(2885..3265)
                     /gene="queD"
                     /locus_tag="P5573_03200"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HRT27103.1"
                     /translation="MAGFFEINVKTDFSAAHCLREYDGDCERLHGHNWTVELSVRCRG
                     LNEIGIGVDFRDAKKALKEVVGRLDHSHLNELPAFQEENPTSENIARFLYGELSRKLN
                     ADGVKVSQVKVYETAGAGVCYWEE"
     gene            complement(3291..4673)
                     /locus_tag="P5573_03205"
     CDS             complement(3291..4673)
                     /locus_tag="P5573_03205"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRT27104.1"
                     /translation="MPKIIKILSLFLLIAASSFSFVHAQERAFTAEYVYAAGKLDDRA
                     TARVLALEGVKDRLWRKLWDDLDGLVLVKQLNLTAEQMAAVVPSLAEVLVVDERWDGR
                     TYRVEAELFVGNLNSLQQSLLTLSRDEGRLKELAEGRKRREDALGRMAVLNAEATTAP
                     GGAKEGLIGRYRLLARQLSESDLMLKGSIAERSGRWREAKKAYDEALKLNPRNESALY
                     RRGIASAQMGMRNDALRDFNRVLKANPESDSALYMRGIVYARMGKYRQAVADLSRAIK
                     RNDRFAPAYYQRAMALDRLGKTGEAVEDLTRAIELNGENSQYYLTRGALLEKLGRRVE
                     ASQDLTRAVKLDQKHELTAAGPLDRSSKDNQAVKELAEAIAKNPTDSEAYYRRGMFYS
                     NIGDCLSAVSDFNKAIELNPKDAASYFMRGVTYGMIDLSYDSLKDIRVSARLGYKPAR
                     EYMSARGLKW"
     gene            4857..>5905
                     /gene="glmM"
                     /locus_tag="P5573_03210"
     CDS             4857..>5905
                     /gene="glmM"
                     /locus_tag="P5573_03210"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HRT27105.1"
                     /translation="MRKLFGTDGIRGLANVPPMTIETVMKIGRATARVFKNKGKRHRI
                     LVGRDTRLSGSMIESALVSGICSMGVDAMTVGPLPTPAVSFLTVNMRADAGMMISASH
                     NPFQDNGIKIFSGDGFKLPDEMEEEIERFVFSDVKEQDLPTGGDVGRAVAIGNAEGRY
                     IAYLKNTFPRKMTLDGMKIVVDCANGAAYKVAPLIFEELGAEVIPIGTHPDGRNINLD
                     CGAMHPEVVCAAVRKHRADAGIALDGDADRVIFSDEKGRKIDGDHIMAVCALDMKAKN
                     TLKKDTVVATIMSNMGFDIAMKREGIHVVKTQVGDRYILEEMLRGGFNLGGEQSGHII
                     FLDHSTTGDGILSAL"
BASE COUNT         1135 a         1780 c         1668 g         1322 t
ORIGIN      
        1 agtcgcacat cgttgttgtt gtaatacatg gcgacgcgca tcgccgtctc cggggatcag
       61 gtcgatttct tcaggctggt gaaaagttta tgagcttctt tcaccgaggc gttttcgtgg
      121 atgatggcgt ggagcgcccg catcatggcg acgggctgcg ggttctgcca gacgttcctc
      181 ccgaggttca ccccgacggc gccccgctgc atcccgtcgt ggacgaattc caggacctcc
      241 agttccgtct cgcacttcgg cccgccagcc atcacgaccg ggaccgggca tccctcgacg
      301 accttgtcga agtttttgca ccagtaggtc ttgacgaccc gggcgccgaa ttccgccgcg
      361 atgcggcagc tcagtgagag ataccgggcc tcctgtttct ggagttcctt gcccaccgcc
      421 gtcacggcca tcacagggat gccgaagtcc tcacagtcgt tgacgatatc ggccaggttc
      481 atcaacgact cgtattcata gtcgcttccc acgaagatcg ataccccgac ggcggtcgcg
      541 ttcaagcgga tgatctcctc gatggaggtc gtgatgccct cattcgccag gtccctgccg
      601 atcacgctcg acccgccgga gacccggagg atgatgggtt tactcttcgc agggtctatg
      661 gcggagcgaa gcacgcccct ggtcacgaag agtgcatcgg cccaggggag gagcggcgct
      721 atggtctcac cgggtctttc caggcagctt gtcggtccct ggaagtagcc gtggtcgatg
      781 gggaggaaga aacaacggcc gtcttctttg aaaagctgcg acatgcggtt cttcataccc
      841 cagtccatcg ttcccaacat ggtctccacc tccaccctcc tatttgccct gataggtctt
      901 gttgagttcc ttgatgacct tgtccgtgag ggagttttcc ttcgaggaat agagggcggc
      961 ggattcatcc aggatggccg cgtacttttc cctttgacct atggccttta cgactttaat
     1021 gatttcaggg aacagcttgt tcgccataaa ctggtccttt gcccggagct cctcagcgga
     1081 gtccttgacc agtctttcgt attccctgta ctcgacctgg tattgcatct ccttttcctg
     1141 catggcctga ggtgtcagca ccgagcgctg tttatcgagg ttttcttttg ccttcttgag
     1201 ttctttttcc ttttgctgga tggcggcctt ctttccctct acagcttttt ctaattccgc
     1261 ctttattgcc ttccccgcgt cggaacgatc tatgatctcc ctcatgttga tgaagccgat
     1321 cttttcggcg gccgaggcgg aaacactgct caggagcagg accgaaaaga cgatgaggca
     1381 tgggatcaga ataaattttt tcatcttatg tctccttttg gttgttttac ccccatgagt
     1441 gccgtccccg tcttttgccc ttcggtgggc ggcgagttga agagaacctt aatcgttttg
     1501 attaagtatg tcaagggcgg gtttgaatcg gccgctcttt ccctcaggcg taaaccgtcg
     1561 ggtcttcgac gccggcctcg cggaaacctt tcttcctgag aagacagctg tcgcatcggc
     1621 cacaggcctt gccttccggc gacgggtcgt agcaactgtg ggtcagtgag tagtccacgc
     1681 cgagggcttt gcccttcagt ataatctgcg cctttgagag gttgatgagg ggggcgtgga
     1741 tctttacccg gaaccggcct tccacggcag ccttgatagc taggttggcc atctcttcga
     1801 aggcccggat gtactcggga cggcagtcgg ggtagccgga atagtcgatg gcattgacgc
     1861 cgacgaagat atccggcgct tccaggacct cggcccaggc gagggcatag gaaagaaaga
     1921 tggtgttccg cgccggcacg taggtgacgg gtatctcctg acgcatctcc cctccggtcc
     1981 gggatttggg gaccgggatg tcggctgtca gggccgagcc tccgatcttg cgcaggtcga
     2041 gacggatcag gaggtgctct ttcgcgccga aggcctcggc gaccctgcgc gccgcgtcga
     2101 gctccacggc atggcgctgg ccgtagccga aggtaaggct gtagagctca tacccctcct
     2161 cgcgggcgac ggccatcgcc gtcgtcgagt cgatcccccc gctcgtgagc acgaccgcct
     2221 ttctcttcat ccggccaccc taaacaccgc ggtttttctc gggccataac accctgtgga
     2281 gctggagctg gagcctgacg aagagcctgt cttccagtat ccaacccgcg agcctgcggg
     2341 gctccatccg gcccgagagc ggcgagaaaa ggaccgtgct gcccggacac ctttcccgga
     2401 tcagcggcaa caccttgcag gcatactcat aatcggcgcg gtcgccgacg acgaacttga
     2461 tttcgtcttt ctccgtcagt cgctcaagat tgcggaagtt gttgcagccg ctctctccgc
     2521 tggaggggca cttcaggtcg acgaccttta cgcagcgcct gtccaccgcg cccacgtcca
     2581 tgctgccgtt cgtctccagg aggaccgcga gtccttcgtc gaggaggcgg cggatgagtt
     2641 cggtcgtctc ctcctgaatg aggggttcac cgcccgtgat ctcgacgaga cggcagccgt
     2701 aacccttcac ccgttcgagg acctccccga tgtccatggc ggcgccctct tcccgggcat
     2761 attccgtgtc gcaccaggag caacggaggt tgcaacctgt tagacgcacg aagacgcagg
     2821 ggaacccggc ataggaggac tcaccctgga tgctgtagaa gacctcgttg accttgaggg
     2881 acaatcattc ctcccagtaa cagacgcctg ctcccgcggt ctcgtaaacc ttgacctgcg
     2941 agaccttcac cccgtcggcg ttgagtttcc gcgagagttc gccgtagaga aaccgggcga
     3001 tgttctccga ggtgggattt tcctcctgga aggcaggcaa ctcgttgagg tggctgtggt
     3061 cgagtcggcc gacgacttcc ttcagtgcct tttttgcgtc ccggaagtcg acaccgatgc
     3121 cgatctcatt caaaccccga catctgacag atagttcgac ggtccagttg tggccgtgga
     3181 gccgttcgca gtcgccatcg tactccctca gacagtgggc cgccgagaag tccgtcttca
     3241 catttatctc aaaaaaaccg gccatagttc tctcccttca aacgctaagt tcaccacttg
     3301 agtccccggg cgctcatgta ctcacgggcc ggtttataac caagccttgc cgatacgcgg
     3361 atgtccttca gggaatcgta agataggtca atcataccat aggtgacgcc ccgcatgaaa
     3421 taggacgctg cgtccttcgg gttcagctcg atcgccttgt tgaagtcact gacggccgac
     3481 aggcaatcgc ctatattact gtagaacata ccccgccggt aataggcctc actgtcggtc
     3541 ggattcttgg cgatggcctc ggcgagctcc ttaaccgcct ggttgtcttt agaggaccta
     3601 tcgagcgggc cggcggcggt caattcatgc ttctggtcga gcttgacggc ccttgtgagg
     3661 tcctgcgagg cctctacgcg ccgtccgagt ttctcaagga gggcaccccg ggtcaggtaa
     3721 tactgggagt tttcgccgtt cagctcgata gcgcgtgtca ggtcctcgac ggcctccccg
     3781 gtcttaccga ggcggtccag tgccattgcc cgctggtagt aagcgggggc aaacctgtca
     3841 tttcttttga tggcgcgcga aagatcggcc actgcctgcc ggtatttacc catgcgggca
     3901 tagacgatgc ctcgcatgta cagggctgag tcgctttcgg ggttagcctt caaaaccctg
     3961 ttgaaatcgc ggagcgcatc gtttctcatc cccatttgcg ccgaggctat cccccgacgg
     4021 tagagggccg attcgttccg tgggttcagc ttcagcgctt cgtcataagc cttcttcgcc
     4081 tcccgccacc tgcccgacct ttcggcgatg gaacccttga gcatcaagtc cgactcgctg
     4141 agttgtcgcg ccaggaggcg gtacctcccg atgaggcctt ccttcgcccc tcccggtgct
     4201 gtcgttgcct ctgcgttgag aacggccatt ctcccgaggg catcttcccg tcttttcctg
     4261 ccctctgcga gctccttcag ccgtccttca tccctgctca aagtcagaag ggactgttga
     4321 aggctgttta ggttgcccac gaaaagttca gcctcgaccc tataagtcct cccgtcccat
     4381 ctttcatcca caaccaggac ctctgcgagg gacgggacga cggcggccat ctgctcggca
     4441 gtgaggttca actgcttgac aaggacaagc ccatcgaggt cgtcccacag ctttctccag
     4501 agacggtcct tcacgccctc cagggcaagc acccgcgcgg tcgccctgtc gtccagcttt
     4561 cccgccgcgt aaacgtactc ggccgtaaat gccctctcct gggcatgcac aaaagaaaag
     4621 gaggatgccg caatgaggag aaataacgac agtattttta tgattttcgg catcggatgt
     4681 accgggcaac ttcaaagcgg acattagaga cttatttttt cgcattgtcc ggcctcccaa
     4741 cccgcctgtc aaggggaaat gacgggactc caagaatccc ttttgcgcct tgttgacaaa
     4801 gaagaaacga aacgataata gatgcgaccg acggtttaaa aatacaggga tcgggtatga
     4861 ggaagctttt cggtacggac ggcataaggg gcttggcaaa cgtccctccc atgaccatcg
     4921 aaacagtcat gaagataggg cgggcgacgg cccgtgtttt caagaacaag ggcaaaaggc
     4981 acaggatact cgtggggaga gacacccgtc tttccggctc catgatcgaa agcgcccttg
     5041 tctcgggcat ctgttccatg ggtgtcgacg ccatgacggt aggtcccctt ccgacgcccg
     5101 cggtttcctt cctgaccgtc aacatgaggg ccgatgccgg aatgatgatc tcggcctccc
     5161 acaacccgtt tcaggacaac ggcatcaaga tcttttccgg cgacgggttc aagctgcctg
     5221 atgaaatgga agaggagatt gaacgctttg tcttctctga tgtgaaggaa caggacctcc
     5281 ccaccggagg tgatgtcggc cgggccgtgg cgataggtaa tgccgaaggc cggtacatcg
     5341 cctacctgaa gaataccttc ccccgcaaga tgaccctcga cgggatgaag atcgtggtcg
     5401 actgcgccaa cggcgctgcc tacaaggtcg cccccctcat cttcgaggaa ttgggcgcag
     5461 aggtgatccc catcgggacc caccccgatg ggcgtaacat caatcttgac tgtggcgcca
     5521 tgcaccccga agtcgtctgc gcagcggtca ggaagcacag agccgacgca gggatcgccc
     5581 tcgacggcga cgccgaccgg gtgattttct cggacgagaa gggcagaaag atcgacggcg
     5641 accacataat ggccgtctgt gccctcgaca tgaaggccaa gaataccctg aagaaagaca
     5701 ccgtcgtcgc gacgatcatg agcaacatgg gtttcgatat cgccatgaag agagagggga
     5761 ttcacgtcgt caagacccag gtcggcgacc gctatatcct ggaggaaatg ttacggggag
     5821 ggttcaatct cggcggcgag caatcaggcc acatcatttt tttagaccac tccacgacgg
     5881 gcgacggcat cctttcggcc ctcca
//
LOCUS       DAOTMX010000076         5904 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_11491_length_5904_cov_3.543341, whole genome shotgun sequence.
ACCESSION   DAOTMX010000076 DAOTMX010000000
VERSION     DAOTMX010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5904
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11491_length_5904_cov_3.543341"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(39..1094)
                     /gene="mraY"
                     /locus_tag="P5573_03215"
     CDS             complement(39..1094)
                     /gene="mraY"
                     /locus_tag="P5573_03215"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HRT27106.1"
                     /translation="MHDVFSAFNVFRYITFRTIYATITAMAIVFLLGPWLIRKLQSLN
                     ISQPIRDDGPDSHQAKQGTPTMGGILIIFSVVTSTLLWANLTVEYVWFALLVTVGYGI
                     IGFMDDYSKLVRKNSRGIPGKIRLAGEIAIALFVSTLLYLKPGFSSSITIPFFKTILP
                     DLGWGYIILATFIIVGAANAVNLTDGLDGLATGPAIICFLTYLFFAYFAGNVKIANYL
                     QIPYVAGTGELAVFCGAMAGAGLGFLWYNAYPAQVFMGDVGSLSLGGALGTLAVATKQ
                     EILLAVVGGIFVLETVSVIFQVGWFKISNGKRIFRMAPLHHHFELKGWAEPKVIVRFW
                     IISGILALLAISTLKIR"
     gene            complement(1125..2597)
                     /gene="murF"
                     /locus_tag="P5573_03220"
     CDS             complement(1125..2597)
                     /gene="murF"
                     /locus_tag="P5573_03220"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HRT27107.1"
                     /translation="MNEQVSLVLRVEDVLMATDGHLLKGEAGGVFYGLATDTRKLSGG
                     NLFIPLVGEKFDGHDFLKEAVEAGASGLLLQRGKEDRLSGVGDTVVILVEDTLRALGD
                     IAHFWRGLFTVPVVAITGSSGKTTTKEMIAGILGDGRTVLKSPGNFNNLVGLPLAIFE
                     MRRSHEAVVLEMGTNRKGEIGRLTEIAAPDVGVITNIGPAHLQGFGSLEAVREEKGDL
                     FRVMANKGMAVINCDDENVRVLARAWDGERITFGVGEEAYVKAGRVTSRGSEGVRFTL
                     RIGELEKDVRLPVVGGHNVRNALAAAAASWVLGESMDSICRGLESFEPLGGRMTVRRL
                     KNEAFLVDDSYNANPVSVSEALKTLRDLKGAHRGIAVLGDMLELGEEAENLHVEIGRI
                     AAETGVDTLILKGTYSRAVASGAMERGLPRERILFMEDPGEIVDKLKGSLQKCDWVLV
                     KGSRKMRLDEVGSFLVDAVGLHEAPPDREGVIFGEEVSKR"
     gene            complement(2604..4124)
                     /locus_tag="P5573_03225"
     CDS             complement(2604..4124)
                     /locus_tag="P5573_03225"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HRT27108.1"
                     /translation="MKLTELIRGLDIVAVKGDRGLDVVDICYDSRRCREGSLFVAVSG
                     LKHDGHDYIGQAVCNGAAAVVLEKEVAVPAGVAAVRVRDSRRTLGLLGRRFFRNPSGS
                     LCVVGVTGTSGKTTLTFLLESIFREAGSSVGVLGTVNYRFGGKTMPAPNTTLESLDLQ
                     RILREMADSGVQNVVMEVSSHALAQHRVDDCEFDIGVFTNLSSEHMDYHKTMEEYFLA
                     KRRLFDTLLDGRKGNRRWRMVVNVDDPWGKRLLGEVGAEAVTFGMDPAADVTCEDASF
                     SLEGIRAAAKTPAGRVMIRSGMIGRFNLSNILAAVAASYALDVPTEAVEAGIRKATGI
                     PGRLEKVGDDGDPSVFVDYAHKEAALRAVLENLSPFKEGRLITVFGCGGDRDRQKRPL
                     MGKAAVALSEITILTSDNPRSEDPLEIIREIEKGINSGAARKLEKDALGRNFDRGYLV
                     IPDRRDAIEFAVRLARRDDIVLIAGKGHETYQIIGDRTIPFDDREVVREALAARRR"
     gene            complement(4124..>5904)
                     /locus_tag="P5573_03230"
     CDS             complement(4124..>5904)
                     /locus_tag="P5573_03230"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRT27109.1"
                     /translation="ILMDSVYADPSKIADQERCAQQLAPLLGEDAKAIRNKLLNSKYF
                     CWLSRQVSPELAQRVRDAKIEGVYLVKEPKRFYPNRELAGPLIGFVGRDAEGLEGIEL
                     KYDQYLKCQSDKMVWARDAKGKRLYLKESLVSEKPDKGYNIWLTIDSRIQYLVELHLK
                     EAIKRTGAKGGMAIVMDPRTGAILALVSEPGFNPNTFFRGRNEVMKNKAIADCFDPGS
                     IFKPFVAAAALEEGVVKETDTFYCENGAYHIANRTIHEAQRKRYGHLTLREVIKYSSN
                     IGSVKVSQRLTKEKFYRYIREFGFGAKTGIDLPGEISGLLRPYERWTNVDAATIAFGQ
                     GVSVTAIQLITAMSAIANGGVLMKPYIVRAVTDPNGNAVEEYKPATVRRVISTSTAKR
                     MISILTDVVGADDGTGKRARLTDVTVAGKTGTSQKFDFSTGRYSSARVRTSFLGFLPA
                     EDARVTILVTLDEPQRDKWGGVAAAPVFKSIGEQILRCYDSRPGEGQIMIEEREEQLQ
                     GGGLKLVAAEAAVVTAATPATMSSEEALTPDFTGMTLKKALEVSRKRGIQLKVIGSGW
                     AVSQEPAPGTTLLENPVCTVRFSTGY"
BASE COUNT         1206 a         1883 c         1540 g         1275 t
ORIGIN      
        1 cttgaaccaa ccttttaaaa cctgtggtcg agccgtcctc accttatctt caacgtgctt
       61 atcgccaaca gagcgagtat cccggagata atccagaatc tcactatgac cttgggttcc
      121 gcccatccct tcagctcaaa atggtgatgg agaggggcca tccggaagat gcgttttccg
      181 ttcgatatct tgaaccaccc cacctgaaaa atcaccgaga ccgtctcaag aacaaaaatc
      241 ccgccgacaa cggcgagaag gatttcctgc ttggtcgcca cggccagggt acccagcgcc
      301 ccgccgagcg acagggagcc cacgtcgccc atgaacacct gagcgggata ggcgttgtac
      361 cagaggaaac cgaggcccgc ccccgccatg gcaccgcaaa agactgcgag ttcgcccgtc
      421 cccgccacgt aaggaatctg cagataattg gctatcttga cgtttccggc aaagtaagcg
      481 aagaacaggt aggtcaagaa gcagatgatg gcgggaccgg tggccaggcc gtccaggccg
      541 tcggtcaggt tgacggcatt cgcggcaccg acgatgatga aggtcgcaag gatgatatac
      601 ccccacccca ggtccggcag gatcgtcttg aagaagggga tcgtgatact cgaactgaat
      661 cccggcttta gataaaggag ggtgctcaca aaaagggcga tcgcgatctc gcccgccaac
      721 cttatcttcc cggggattcc cctgctgttt tttctgacga gcttactgta gtcgtccatg
      781 aacccgatga tgccgtaacc gacagttacg agaagggcga accaaacgta ctccaccgtc
      841 aggttcgccc acaggagcgt agaggtaact actgaaaaga tgatcaagat accgcccatg
      901 gtgggcgtcc cctgtttggc ctggtgggaa tcaggcccgt cgtcgcggat cggctgactg
      961 atgttcagag actggagttt tcgtatcagc cacggaccga ggaggaaaac gatcgccatg
     1021 gccgtgatag tcgcgtagat cgtccggaag gtgatgtacc ggaagacatt gaaggcggaa
     1081 aaaacatcat gcaacggata gagaagatga tagaccaatg cgtattacct cttcgatacc
     1141 tcttccccga aaatcacgcc ttcgcggtcg ggcggcgcct catgaagacc gaccgcgtcg
     1201 acgaggaagc tccccacttc gtcaagcctc attttcctcg aaccttttac caggacccag
     1261 tcacactttt gcagactgcc cttaagtttg tctactatct cccccgggtc ttccatgaaa
     1321 aggatacgct ccctcggcag tcccctttcc atcgccccgg aagccacggc ccgggaatag
     1381 gttcccttca ggatgagtgt gtccacaccg gtctcagcgg caatcctacc gatttctacg
     1441 tgcaggttct ccgcctcctc tccaagctcc agcatgtcgc cgagaacggc aatgccccga
     1501 tgcgcccctt tcaggtcccg aagcgttttc aaggcctcgc tcaccgacac cgggttggcg
     1561 ttgtagctgt cgtcgaccag gaaggcctcg ttcttcagcc tcctgaccgt catcctgccg
     1621 cccaggggct caaaagactc caacccccga cagatggaat ccatgctttc ccccaggacc
     1681 caagaggccg cggcggcggc aagggcgttc ctcacgttgt gcccgccgac gacggggagg
     1741 cggacatcct tctcgagttc gccgatccgt aaggtgaacc ggacgccttc actgccgcgg
     1801 ctggtgaccc ggccggcctt cacgtaggcc tcttcaccga cgccgaaagt gatccgttcg
     1861 ccgtcccatg cccgcgcaag aactctcacg ttttcgtcgt cacaattgat gacggccatg
     1921 cctttgtttg ccatcacccg gaaaagatca cccttctcct cccgcacggc ttcaagggaa
     1981 ccaaaacctt ggagatgcgc tggcccgatg ttggtgatca cgcccacatc tggcgcggcg
     2041 atctccgtca ggcgtccgat ctcccctttt ctgttggtgc ccatctccag gacgacggcc
     2101 tcgtgagaac gcctcatctc gaagattgcc agaggaagcc cgacgaggtt gttgaaattt
     2161 cccggactct tgagaacggt acgaccgtct ccaaggatac ccgcgatcat ctctttcgtc
     2221 gtcgttttcc ctgagctgcc cgtaatggct acgaccggca cggtgaagag ccccctccag
     2281 aaatgggcga tgtccccgag cgcccgcagt gtgtcctcga caaggataac cacggtgtcg
     2341 ccgaccccgc tgagcctgtc ttcctttccc ctctgtaaga gaagcccaga cgcaccggct
     2401 tcgacggcct ccttcaaaaa atcgtggccg tcaaactttt cgcccacgag ggggatgaag
     2461 aggtttcctc cggaaagttt ccgtgtatcc gtagcaaggc cataaaatac cccccccgcc
     2521 tcgcccttca gcaggtgccc gtccgtagcc atcaggacat cctcgaccct caggacgagg
     2581 gaaacctgct cgttcaaagc ctctcaccgc ctccttgcag ccaaagcttc cctcacgact
     2641 tctctgtcgt cgaagggaat cgtccggtct ccgattatct gataagtctc atgacccttg
     2701 ccggcgatga ggactatgtc gtcccgccgt gccagcctga cggcgaattc aatggcgtcc
     2761 ctgcggtcag gaatgaccag gtatcccctg tcgaagttcc tccctagggc gtccttttcc
     2821 agttttctgg cggcacccga gttgatcccc ttctctatct cccggatgat ctccaggggg
     2881 tcttccgatc gggggttatc ggacgtcagg atagtgatct cgctcaaagc aaccgccgcc
     2941 ttacccatga gcggcctctt ctgccggtcc cggtcgccgc cgcaaccgaa gaccgtgatg
     3001 agccgccctt ctttgaaggg ggagagattt tccaggaccg ccctcagtgc ggcctcctta
     3061 tgggcgtaat cgacaaacac ggaggggtca ccgtcatcac ccaccttctc gagcctgccg
     3121 gggattccgg tcgccttcct gatgccggcc tcaacggctt ccgtcggtac atccaaggca
     3181 taagaggcgg caaccgcggc gaggatattg gacaggttga accggccgat catccctgaa
     3241 cggatcatga cccggcccgc cggcgttttc gccgcagccc tgatcccctc aagggaaaat
     3301 gaggcatctt cacaggtaac atcggccgcc ggatccatgc cgaaggttac tgcctctgca
     3361 ccaacctccc cgaggagcct tttcccccag ggatcgtcca cattcacgac catcctccat
     3421 ctccggtttc ccttgcggcc gtcaagcagt gtgtcgaaca accttctctt cgccaggaaa
     3481 tattcttcca tcgtcttgtg gtaatccatg tgttcggaag agaggttcgt gaagacaccg
     3541 atgtcgaatt cgcagtcgtc tacccggtgc tgagcgaggg cgtgggagga gacctccatg
     3601 accacgttct gaacgccact gtccgccatc tcccggagaa tcctctggag gtccagggat
     3661 tccagcgttg tgttgggggc gggcatcgtc ttaccgccga agcggtaatt gacagtcccc
     3721 aagaccccga cgctggaacc ggcctccctg aatatcgatt ccagaagaaa ggtcaaggtc
     3781 gtcttaccgc tcgtcccggt aacccccact acgcagaggc tgcccgaggg gttgcgaaag
     3841 aatctccgtc cgagtaggcc caaggtacga cgactgtcgc ggacccggac cgcggccacc
     3901 cctgcgggaa ccgccacctc tttctccaaa acgacggccg ccgcaccatt acacacagcc
     3961 tgcccgatgt agtcatggcc gtcgtgtttg agtcccgaaa ccgccacgaa gagcgaacct
     4021 tcgcggcatc ttcgggaatc atagcagatg tcaacgacgt caagtccccg gtcgcctttc
     4081 acggccacga tgtccaggcc acgtatgagt tccgtcaact tcatcagtat cccgtgctga
     4141 accggacagt gcagaccggg ttctccagta gggtcgtacc cggcgccggc tcctgactca
     4201 ctgcccaacc gctcccgatc actttcagtt ggatccccct cttccgggaa acttccagtg
     4261 cctttttcag agtcatgccg gtaaaatcag gggtcagggc ttcttcagaa gacatcgtcg
     4321 ccggtgtcgc cgccgtaacg acggccgctt cagcggcaac gagtttcagg ccgccgccct
     4381 gaagttgctc ttccctctct tcaatcatga tctgtccttc tcctgggcga gagtcataac
     4441 atctcagaat ctgctcgccg atgctcttga atacaggagc cgccgcaacg ccaccccact
     4501 tatccctctg cggctcgtcg agggttacca ggatggtcac ccgtgcatct tccgccggga
     4561 ggaagccaag aaaagacgtc cttacccgcg ccgaggaata ccggcccgtg ctgaaatcga
     4621 atttctgcga tgtccccgtc tttccggcga cggtgacgtc cgtcaagcgc gcccgtttac
     4681 ccgtaccgtc atcggctccc acgacgtccg tcaggattga aatcatcctc ttggccgtgg
     4741 atgtcgagat gacccgcctc accgtagcag gtttatactc ctcgaccgca tttccgttcg
     4801 ggtccgtgac cgcgcggacg atgtaaggct tcataaggac cccgccgttg gcaatggccg
     4861 acatagcggt gatcaactgg atggccgtta cggagacacc ctgaccgaag gcgatggtgg
     4921 cggcatcgac gttagtccaa cgctcgtaag gcctcaaaag tcctgatatt tcaccgggaa
     4981 ggtcgattcc cgtcttggcg ccgaacccga attcccttat ataccggtag aatttctcct
     5041 tcgtcagcct ctggctgacc ttcaccgagc ctatgttgct cgaatacttg atgacttccc
     5101 tcagcgtaag gtgcccgtat cttttccgtt gcgcctcgtg gatcgttcgg tttgcgatat
     5161 ggtaagcgcc gttctcacag taaaaagtat ccgtctcctt tactacaccc tcctcgaggg
     5221 ccgctgccgc tacgaagggc ttgaatatcg aaccggggtc aaaacaatcg gcaatggcct
     5281 tgtttttcat cacctcgttg cggccccgga aaaaggtatt ggggttgaag ccgggctcac
     5341 tcaccagtgc caggattgcc cccgtccggg ggtccatcac gatcgccatt ccgcctttag
     5401 cgccggtcct tttgatcgcc tccttcagat gcaattccac aagatattgg attcgactgt
     5461 cgattgtcaa ccatatattg tatcccttgt ccggtttttc cgagacaagg ctttccttga
     5521 gatataggcg cttgcctttc gcgtcccgag cccagaccat cttgtcggac tggcacttga
     5581 ggtactgatc atacttcagt tctatgccct ccaggccttc tgcatccctt cctacaaagc
     5641 caatcagcgg tcctgcgagt tccctgttcg ggtagaaacg cttcggttcc ttgaccaggt
     5701 agaccccctc gattttcgcg tcccggactc tctgggcaag ctcgggcgac acctgccggg
     5761 acaaccagca gaagtattta gaattcaaaa gcttattgcg aatggctttc gcatcttcac
     5821 ccaggagtgg ggccagttgc tgggcgcatc tttcttggtc tgcgattttg gaggggtccg
     5881 catagaccga gtccatgaga atgc
//
LOCUS       DAOTMX010000077         5883 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_11535_length_5883_cov_3.756692, whole genome shotgun sequence.
ACCESSION   DAOTMX010000077 DAOTMX010000000
VERSION     DAOTMX010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5883
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11535_length_5883_cov_3.756692"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="P5573_03235"
     CDS             complement(<1..1001)
                     /locus_tag="P5573_03235"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HRT27110.1"
                     /translation="MKDVVVVSACRTAIGTFGGTLKDLNGPTLASVVMKEAVKRAGID
                     AGAVNDVRFGCCVEHHDNLNVTRVGALLAGIPETVPAVTVNRVCISGMEAAISGAAMI
                     WSGLADVILAGGVEHMSGVPYTAPAARWGCRLQDSVFVDALIHALYAGSHIIPGLEKG
                     PVKEGPIVDLFRGKPYIMGVTAELVAQMYNITREEMDAVALRSHNNAERATREGDFKE
                     EIVPVEVPQKKGKPPIIFDKDEHFIPGLTMDRLAALPPAFIPKVGKVTAGNASGINDG
                     ASAMVIMSAEKAKELGVKPIAKVKAVGYGGCHPSVMGLSPAPAVRDLLKKSGLRLSD"
     gene            1189..1794
                     /locus_tag="P5573_03240"
     CDS             1189..1794
                     /locus_tag="P5573_03240"
                     /inference="COORDINATES: protein motif:HMM:NF014078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HRT27111.1"
                     /translation="MPTRQRIPPYMKRGLKKIKFSLALFLYPVRPLLEKVGIRYDLVK
                     IGNDFYREEFCRIPAPGCAVILPHCLIHEECPAKFSKEEGVLCIKCRRCKCGEIKAFS
                     EERGLQFFITPSVGFTKRLAERKNIRAAVGATCIYEIERGLKRVRVSLNGVTYNRTRI
                     IPQMVMTAKYDCTDNDVDWDLLKEIILSRKDRVSVKDVRPR"
     gene            1939..2130
                     /locus_tag="P5573_03245"
     CDS             1939..2130
                     /locus_tag="P5573_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27112.1"
                     /translation="MTTIMPEGEEVQKAIKWISQNLEENPNQPLIPLVEKAVFKFDLS
                     PKDSEFLMTFFRKRKSGGQ"
     gene            2235..3248
                     /gene="amrS"
                     /locus_tag="P5573_03250"
     CDS             2235..3248
                     /gene="amrS"
                     /locus_tag="P5573_03250"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HRT27113.1"
                     /translation="MREALLYEKLEDGKVSCFLCSHRCLIGDGKYGICNVRENRGGTL
                     YTHSYGELIAQNIDPIEKKPLYHFLPGSASYSIATIGCNFQCSFCQNWEISQAREAER
                     LGLRGRKAEPSEIVNRAVQSGSKSISYTYTEPTIFFEYALDVSRLAKEKGLYNVFVTN
                     GYMTGEMLEAMHPLLDAANVDLKSFSKDFYRRFCKGNLDPVLRNIEKMKKLNIWVEVT
                     TLVVPGLNDSDAEFDGIAGFIAGIDKAIPWHISRFHPQYKMEDHASTPMQTMGRALAA
                     GRKAGLHYVYLGNVMSRWNHTYCHRCDECLVERTGFSIASYRIKDGKCPFCGSAVAGV
                     GLG"
     gene            complement(3271..4737)
                     /locus_tag="P5573_03255"
     CDS             complement(3271..4737)
                     /locus_tag="P5573_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HRT27114.1"
                     /translation="MALRAIKRSIKKILIANRGEVALRIMRTAMELGISSVVVYEKPD
                     SDAYFIRIADEAFLLGDGPRKDYLDIDKLIWAAKKTDADAIHPGYGFLSENADFADAC
                     QRAGIIFIGPPSKVIRNLGNKVKAREIMRSAGIPFIPGTYNLSAGEDGVREAMAFGEK
                     HGYPIMLKAAAGGGGRGIRKVDNKEELAVQLRRARVEAKNAFKDDSVYVEKCIENPRH
                     VEVQILADRYGNIMHLGSRDCSIQRRHQKLMEIAPADLPEDVLHAMYDAAVRAARAAD
                     YTNVGTVEFLVDPKTNEFWFMEVNTRLQVEHTVTEELTNVDIVREQILIAEGAALDVA
                     GEKITIAGRAIQVRINAEDPQNNFRPDGGKVVDFYLPPGGPGIRLDGIIYQGYRIPME
                     YDSLLVKMTVRGFDWEQTVARLRRALDLFVISGPQTTIPFYRAICREPDFRSGRFDTS
                     YISTHPQVFNYPEPTEEVKRLKAFITTVHPVVFFPYVY"
     gene            complement(4895..5113)
                     /locus_tag="P5573_03260"
     CDS             complement(4895..5113)
                     /locus_tag="P5573_03260"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HRT27115.1"
                     /translation="MSVELLSPIPGKVMKVLVKVGETVNEDDEVMRIESMKMENPIYA
                     PASGKIKEVLVQENDEVETDDLLLVIED"
     gene            complement(5142..>5883)
                     /locus_tag="P5573_03265"
     CDS             complement(5142..>5883)
                     /locus_tag="P5573_03265"
                     /inference="COORDINATES: protein motif:HMM:NF015910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HRT27116.1"
                     /translation="EAVSIGIIGACDGPTAIYVTSQYAPHLLGAVSVAAYSYMSLVPL
                     IQPPIMKILTTERERTTVMKAHKKTVSPTTKLLFPLVITIVGGLIAPKGLPLLGTIML
                     GNLMRESGAVERLTKASQNEIANVVTLLLGISIGATMDGRVFLQPQTLIVLGLGFVAI
                     CLDTACGVLLGKVLYVFTGGKVNPLIGAAGISAFPMSARVVMREGQKYNKKNYLLMHA
                     MGANAGGQIGSVIAAAVMLSVLKGMGII"
BASE COUNT         1287 a         1662 c         1590 g         1344 t
ORIGIN      
        1 aaatccgaga gtctcagtcc ggacttcttt aagagatcgc gaaccgccgg ggcagggctg
       61 agtcccatga cggagggatg acatcccccg tacccgacgg ctttgacctt ggcgatcggc
      121 tttacaccca attccttcgc cttttcggcc gacatgataa ccatggcgct tgcgccgtcg
      181 ttgatgcccg acgcattgcc cgcggttacc tttccgacct tcggaataaa cgccggcggc
      241 agcgccgcca gtctgtccat ggtcaggccg gggatgaagt gctcgtcttt atcgaagatg
      301 atgggaggct tgcccttctt ctgaggaact tcaaccggca cgatctcttc cttgaagtct
      361 ccttcccggg tggccctctc ggcgttgttg tggctccgga gcgccaccgc gtccatctct
      421 tccctcgtta tgttgtacat ctgggcgacc agttccgccg tgacgcccat gatgtaaggc
      481 ttgccccgga acagatccac gatgggtcct tccttgaccg gtcccttttc caggcctgga
      541 atgatgtgcg acccggcgta aagggcgtgg ataagggcat ccacaaagac gctgtcctgc
      601 aaacggcatc cccaacgtgc tgccggggcg gtataaggaa cgcctgacat gtgctcgacg
      661 ccgccggcca ggatgacatc ggccagacct gaccagatca tcgccgcgcc cgaaatggcg
      721 gcttccatac ccgaaataca gacccggttg accgttaccg ccggaaccgt ttccggtatg
      781 cccgcgagca gggctccgac cctggtgacg ttgaggttgt catgatgttc gacgcagcat
      841 ccgaacctaa catcgttgac cgcaccggca tcgatgccgg ccctcttcac agcctccttc
      901 atcaccacgc tcgcaagcgt tgggccgttc aaatccttta gggttcctcc gaaagtaccg
      961 atcgcggtac ggcaagcaga aacaaccaca acgtctttca tcttcgtcta tcctccttac
     1021 tgtagagtta tctttagatg gccggcaagc attccagtgc cggaaagttg ccgtttcacc
     1081 tcaatggaca actcttataa ttcaagtccg gtccggagta aagaaatctt ctttcttgac
     1141 aggaaatcct gccttcttat aacctcgaag cgaatctgtg tttacaccat gcccacacga
     1201 cagaggatcc caccatacat gaagcgaggg ctcaaaaaaa ttaaattttc actggccctc
     1261 ttcttgtatc ctgtaagacc gctgctggaa aaggtcggca tccgctatga cctggtgaag
     1321 atcgggaacg atttttatcg ggaagagttc tgtcgcattc ctgccccggg ttgtgccgtc
     1381 atcctgcctc actgcctgat tcacgaagag tgtccggcaa agttctccaa ggaagagggt
     1441 gtcctgtgta tcaaatgccg gcgctgcaag tgcggtgaga tcaaggcgtt cagcgaagag
     1501 agggggttgc agtttttcat cacgcccagc gtagggttca ctaaacgact cgcggagaga
     1561 aagaacattc gagccgcggt gggtgcgacc tgcatctacg aaatagaaag gggactcaag
     1621 agggtgaggg tgagcttgaa cggggtcacc tacaacagga ccaggatcat cccccagatg
     1681 gtcatgactg ctaaatacga ctgcaccgac aacgacgttg actgggacct cctgaaggaa
     1741 atcatcctct cccggaagga ccgggtttcg gtgaaagacg ttcgtccccg ctgaggtgtg
     1801 tcctggtcag aaacgcctca tcaccgctga ggcgacggca tgacgtcaaa gtttgctgtc
     1861 aaagtttgct tgaaatggag ggccttttcc ggtatagaaa aggagaccag aggaaatttt
     1921 tcataggagt ccgagaccat gacgacgatc atgcccgaag gagaagaagt acagaaggcc
     1981 atcaagtgga tttcccagaa cctcgaagag aaccctaatc agcctttgat tccgctggtt
     2041 gaaaaagccg ttttcaaatt cgacctgtcc cccaaggatt cagagtttct catgactttc
     2101 ttccgcaaga gaaagtctgg aggtcagtaa gcctccgttc cattccgctc cacactgcta
     2161 gtgtgaagtg tggcatacca catcgctttt tgggcatttg ctcttttcgg caaattccat
     2221 gaaggcggaa cgcattgaga gaagcgctcc tctacgaaaa acttgaagac gggaaggtat
     2281 cctgctttct gtgtagtcat cgctgcctga tcggcgacgg caagtacggc atctgtaacg
     2341 tgagagaaaa ccgcggtggg acgctgtaca cccattctta tggagagttg atcgctcaga
     2401 acatcgaccc catcgaaaaa aagcccctct accactttct tcccggcagt gcctcctact
     2461 ccatcgcaac catcgggtgc aattttcagt gctccttctg ccagaactgg gagatatccc
     2521 aggcccggga ggcggaaaga ctcggcctcc gcggccgaaa agcggaacct tctgaaatcg
     2581 taaaccgggc cgtccagtcc ggctcaaaga gcatttccta tacctacacg gaacctacca
     2641 tcttctttga gtatgccctc gacgtctcca ggctggcaaa ggagaagggt ttgtacaacg
     2701 tctttgtgac gaatggctat atgaccgggg agatgctgga ggcgatgcac ccacttctcg
     2761 acgccgccaa cgtagacctc aagtctttca gcaaagactt ttaccgccgg ttctgcaaag
     2821 gaaaccttga cccggtactc aggaacatag aaaaaatgaa gaaactgaac atctgggtcg
     2881 aggtgacaac tctggtcgtg ccgggtttga acgactcgga cgccgagttc gacggtatcg
     2941 ccggtttcat agccggcatc gacaaggcca tcccttggca tatcagccgc tttcacccgc
     3001 agtataaaat ggaggaccat gcgagtacac ccatgcagac tatgggtcgg gcattagcgg
     3061 ccggaaggaa ggccggcctc cattacgtct acctcggtaa cgtgatgagc cggtggaacc
     3121 acacttactg ccaccgatgt gacgagtgct tggtcgagcg tacgggtttc tccatcgcaa
     3181 gctaccggat caaggacgga aaatgtcctt tttgcggcag tgccgtagcc ggagtcggtc
     3241 tcggctgaaa gatcccgaat taagtttcaa tcaatagacg tacggaaaga ataccacggg
     3301 gtggacggtt gtgatgaatg ccttcagtct tttcacttct tcggtcggtt ccgggtagtt
     3361 gaaaacctgc ggatgggtcg agatgtaact cgtgtcgaac cgtcccgacc tgaaatccgg
     3421 ctccctgcag atggcgcggt agaaggggat ggtggtttgc ggcccggaaa tgacgaataa
     3481 gtcgagggcg cgcctcagcc ttgcaactgt ttgctcccag tcgaaccccc ggacggtcat
     3541 cttgacgagg agcgaatcgt actccatcgg gatccggtat ccctggtaga tgataccgtc
     3601 aagtcggatg ccgggcccgc cgggggggag gtagaaatca acgacctttc ccccgtcggg
     3661 cctgaagttg ttctggggat cctcggcgtt gatccggacc tggattgccc taccggcaat
     3721 cgtgatcttc tccccggcga cgtccagagc cgcaccttcg gcgatcagga tctgctcccg
     3781 tacgatatca acattggtca gctcttcggt tacggtatgc tccacctgaa gccgggtgtt
     3841 gacctccatg aaccagaact cgttcgtctt agggtcgaca aggaactcga cggtcccgac
     3901 gttggtgtag tcggcggcgc gggcggcgcg gacagcggca tcgtacatgg catgcaggac
     3961 atcttcggga aggtccgctg gcgctatctc cataagtttt tggtgccgcc tctggatgga
     4021 gcagtcccgg cttcccaggt gcatgatgtt accgtatcga tccgcaagaa tctgcacttc
     4081 aacatgtctt ggattctcga tgcacttttc cacatagacg ctgtcgtctt tgaaggcgtt
     4141 cttcgcctcg accctcgccc gccggagctg tactgcgagc tcttccttgt tatctacttt
     4201 ccggattccc cgccctccgc cccctgccgc ggccttgagc atgatgggat agccgtgctt
     4261 ctcgccgaag gccatcgcct cacggacgcc gtcttctccg gcggatagat tgtacgtacc
     4321 cgggatgaag gggataccgg cacttcgcat gatctccctt gcttttacct tgttgcccag
     4381 gttccggatc acctttgaag gaggtccaat gaagattatc cccgcccgtt ggcaggcgtc
     4441 ggcgaaatcg gcattttccg aaagaaaccc gtagccggga tggatggcgt cggcatccgt
     4501 cttttttgcc gcccagataa gtttatcgat gtcgaggtag tctttcctcg ggccgtctcc
     4561 gagcaggaag gcctcgtcgg caatgcggat gaagtaggcg tcgctgtcgg gcttttcgta
     4621 aacaacgacg gaggagatgc ccagctccat ggctgtccgc atgatgcgca gggcgacctc
     4681 accgcggttg gcgatgagaa tttttttaat acttcttttg attgccctta aagccatggg
     4741 gagttgaatt ctgtgaccgt cattgtgtct caatccgtga gagcgatacg aaccacggct
     4801 ccaataatct attgtcggga ccggaccccg gtgatcatac cgttacgcaa ggagcccgaa
     4861 aggccccgag ggttacgaat acgttccccc gtttctaatc ttcgattacc agaagcaagt
     4921 catcggtctc cacttcgtcg ttctcctgga caaggacctc ttttatcttc ccggaagcag
     4981 gtgcgtagat ggggttttcc attttcatcg actcgatccg catgacttca tcgtcctcgt
     5041 taaccgtctc acctacttta actagaactt tcatgacctt tcccggtata ggcgataaga
     5101 gctcaacact cacgtttttt gcctcctgta aaaatttcca tctatatgat tcccataccc
     5161 ttgagtaccg atagcatgac cgccgccgcg atcaccgagc cgatctgtcc gccggcattg
     5221 gcacccatgg cgtgcatcaa gagataattc ttcttgttgt atttctggcc ctctctcatg
     5281 actacccggg cggacattgg aaaggctgaa atccccgcgg ccccgatgag cgggttgacc
     5341 ttgcctcctg taaaaacgta gagaaccttt cccagaagaa ccccgcatgc cgtgtcgagg
     5401 cagatcgcga caaaaccaag accgaggacg atcagcgtct gaggctggag gaagacccga
     5461 ccgtccatcg tcgccccgat cgaaatcccc agaaggagag tgacgacgtt ggcgatctcg
     5521 ttctgcgagg ccttcgtcaa acgttcgacg gcacctgatt cacgcatcag attgccgagc
     5581 atgatcgtcc ccaggagggg caagccctta ggcgctatca gccctcccac gatagtgatg
     5641 acaaggggaa agagaagctt ggtcgtcggg gatacggttt tcttgtgagc cttcatgacc
     5701 gttgttcgct ctctctccgt tgtgaggatt ttcatgatcg gcggctggat cagggggacc
     5761 agggacatgt aggaataagc cgcgaccgaa acggccccca agagatgggg cgcgtactgg
     5821 gaggtgacgt agatcgccgt cggtccgtca caggctccga ttatgcctat ggagacagct
     5881 tcc
//
LOCUS       DAOTMX010000078         5882 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_11538_length_5882_cov_4.306333, whole genome shotgun sequence.
ACCESSION   DAOTMX010000078 DAOTMX010000000
VERSION     DAOTMX010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5882
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11538_length_5882_cov_4.306333"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1589)
                     /gene="gyrA"
                     /locus_tag="P5573_03270"
     CDS             complement(<1..1589)
                     /gene="gyrA"
                     /locus_tag="P5573_03270"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HRT27117.1"
                     /translation="MEMLYQRNVPINIEDEMRKSYMDYAMSVIIGRAIPDIRDGLKPV
                     HRRALYAMWEMGNVWNKPYKKSARIVGDIIGKYHPHGDAAAYDTIVRMAQDFSMRYPL
                     IDGQGNFGSIDGDPPAAMRYTEIRMARIAEELLADLEKDTVDFVPNYDSSLREPSVLP
                     ARFPNLLLNGSSGIAVGVATNIPPHNLNEVVDGTIALIRNPGITVEELMHYIQGPDFP
                     TAGFINGREGIVSAYKTGRGTIRVRARAVIERNARNDRESIIITEIPYLVNKANLIER
                     ISELVKEKKMEGIADLRDESDREGIRIVVDLKKDEPSGVILNQLYKHTQMETTFGIIM
                     LSIDKGQPRVLNLKDILQKFLDFRKEVIIRRTMFDLARAKERAHILEGLIVALGNIDA
                     VVALIKAAKNPQEAADGLCRRFGLTEIQARAILDMRLQRLTGLEREKIDAEFKELTEL
                     IRRLQGVLDDQQKVLDVIVGELEEIKKKYGDERRTEIVQSVDDIDIEDMIVEEDMVVT
                     VSHSGYIKRNPVSLYRSQHRGG"
     gene            complement(1772..2104)
                     /locus_tag="P5573_03275"
     CDS             complement(1772..2104)
                     /locus_tag="P5573_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27118.1"
                     /translation="MKAAATLFVALALVIASGVSGPAFGKEPDIPVPADILIVRPVSL
                     AATVVGTALFIVSLPFSIPSDSVETVAKKLVEEPFRYTFMRPIGGFDEDREIDAAKEK
                     SKDAKDVR"
     gene            2381..2521
                     /locus_tag="P5573_03280"
     CDS             2381..2521
                     /locus_tag="P5573_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27119.1"
                     /translation="MKEIDVRDENIRKETLGTVDFYDWLDTFEHKLRPKVEKSLPNVS
                     GK"
     gene            complement(2533..4032)
                     /locus_tag="P5573_03285"
     CDS             complement(2533..4032)
                     /locus_tag="P5573_03285"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF010094.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA];
                     GO_process: GO:0035999 - tetrahydrofolate interconversion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine hydroxymethyltransferase"
                     /protein_id="HRT27120.1"
                     /translation="MVASPVVEYLVGKKAEEIKPEFLAFLANLEVVSRVSPPVAKAVI
                     DELEAQRTNLKLIASENYSSLSTQFAMGNLLTDKYAEGFPGHRFYAGCENVDAVESLA
                     ATEACKLFGCDHAYVQPHSGADANLVAYWAILSARVEMPALEDLGETNLSKLTAQQWE
                     SLRVRMGNQRLLGMDYYSGGHLTHGYRNNVSARMFQAFTYTVDRDTGLLDYAAIEKQA
                     LEVRPLILLAGYSAYPRAINFRKMREIADRAGAVLMVDMAHFAGLVAGGVFTGDYSPF
                     PFAQVVTTTTHKTLRGPRGGVVLCSGEFADAVDRGCPLVLGGPLPHVMAAKAVAFVEA
                     NRPEFKAYAGKIVENSRAMVEECLRLGMRVATGGTDNHLFLIDVSPFGITGRQAESAL
                     RQCSITLNRNALPFDPNGPWYTSGLRVGTPAVTTLNMGREEMKEIASIIRLVLDNTNP
                     LTAGGAKSQAKYEVDPKAEAEAKGRVRAVMSQYPVYPEIDLDFLKKHFR"
     regulatory      complement(4060..4139)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01750"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ZMP/ZTP riboswitches appear to regulate genes
                     involved in one-carbon metabolism; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="5-aminoimidazole-4-carboxamide
                     ribonucleotide (ZMP)/
                     5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl
                     5'-triphosphate (ZMT)"
                     /db_xref="RFAM:RF01750"
     gene            complement(4217..5161)
                     /locus_tag="P5573_03290"
     CDS             complement(4217..5161)
                     /locus_tag="P5573_03290"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRT27121.1"
                     /translation="MKGRKAGGLGVVLIVIIVALVLFFILFPGTVFRAAQYVNRSLSG
                     LSEHSVQVDDSRIVYLEGGRGENILLLHGYTADKDNWTAFAKYLTPKYHVVIPDFPGF
                     GESSRHQEASYDIESQVERLHRFVQVMRLGKVNVAGNSMGGTIAAVYAAKHPQEVLSL
                     ALLAPGGIQGGVKSELMKMFEKGVNPLVIKSDDDFERMMRLAFVKVPAIPYPVRKAIV
                     ARAIKDGPFNEKVMADMLRKPISLESYLPLIKAKTLILWGDRDNILDVSCVPIIEKRI
                     KDHQTVIMKDCGHMPMLERPEETAGHYLKFLQGGAGRK"
     gene            complement(5215..>5882)
                     /locus_tag="P5573_03295"
     CDS             complement(5215..>5882)
                     /locus_tag="P5573_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002379278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HRT27122.1"
                     /translation="LQLVKRVMRIETVLDRFEKEEKNRTVMRILAGDPAFSPEDFQDE
                     AALLLVAKRVRMAVGERLVDYRTEPDPEHSGYKLILDYRMKGQVFTTVLDREIFKSPR
                     FLEVKDLLNQVSALGEPPYTVVDEDGEKKELAGMTALVEHVNALGQKGLTVQRYKGLG
                     EMNPEQLWETTMNPEKRTLLQVKVEDVVIADDIFTTLMGDQVEPRKDFIYKNALYAQN
                     LDV"
BASE COUNT         1113 a         1741 c         1656 g         1372 t
ORIGIN      
        1 ccgccgcggt gctggctgcg gtagaggctt acggggttac gcttgatgta gccggagtgg
       61 gaaaccgtca ccaccatgtc ctcctcgacg atcatgtcct cgatgtcgat gtcgtccacg
      121 ctctggacga tctccgtcct ccgttcatcg ccgtacttct ttttgatctc ctcgagttcc
      181 cccacgatga cgtctaggac cttctgctgg tcgtccagga caccctggag ccttcggatg
      241 agctcagtca actccttgaa ctcggcgtct atcttctcgc gttcaagccc ggtcaacctc
      301 tgcaatctca tgtccaggat ggccctcgcc tgaatctcgg tgagcccaaa cctcctgcag
      361 agcccgtcgg cggcctcctg cgggttcttg gctgccttga tcaaggccac gacggcgtcg
      421 atgttgccga gcgcgacgat gagaccctca aggatgtggg cgcgctcctt cgcccgggcg
      481 aggtcgaaca tcgtccggcg gatgatgact tccttacgga agtccaggaa cttctggagg
      541 atatccttga ggttgaggac acggggctga cccttgtcga tagagagcat gatgatcccg
      601 aaggtcgtct ccatctgggt gtgtttgtaa agctggttga ggatgacgcc ggagggttca
      661 tccttcttta ggtcgacgac gatgcggata ccctcccggt ccgactcgtc ccggaggtcg
      721 gcgatgcctt ccatcttctt ttccttgacg agctcggata tccgctcgat caggttggcc
      781 ttgttcacta ggtaagggat ctccgtgatt attatgctct cccggtcgtt cctggcgttc
      841 ctctcgatga cggcccgcgc cctgacacgg atcgtccctc tgccggtctt gtaggccgaa
      901 acgatccctt ccctgccgtt gatgaacccc gccgtgggaa agtccgggcc ttggatgtag
      961 tgcatcaatt cctcgacggt gatcccggga ttgcggatga gggcgatcgt cccgtcgacg
     1021 acctcgttca ggttgtgggg cgggatgttc gtggcgactc ccacggcgat accggacgag
     1081 ccgttcagca gcaggttggg gaaccttgcc ggcaggacgg aaggctcccg gagggaggag
     1141 tcgtaattgg ggacaaaatc gacggtgtct ttttcgaggt cggcgagcag ctcttcggcg
     1201 attctcgcca tccgtatctc cgtgtaacgc atcgcggccg gcggatcccc gtcgatgctg
     1261 ccgaagtttc cttgcccgtc gatcagcggg taacgcatcg aaaaatcctg ggccatccgc
     1321 acgatggtgt cgtaggccgc cgcgtctccg tgggggtggt acttaccgat gatatcaccg
     1381 actatcctcg ccgacttctt gtagggttta ttccagacat tccccatctc ccacatggca
     1441 taaagggcgc ggcgatgtac cggtttgagg ccgtcccgga tgtccgggat ggcacgtccg
     1501 atgatgacgc tcatcgcgta gtccatgtag gattttctca tctcgtcttc gatatttatg
     1561 ggtacgtttc tctggtaaag catctccatg tcgatacacc tttatcctgg gtcatcattt
     1621 caaaaaagcc tcgtggtatc aaccactaag ccaaactcac gttggaatta taccatcttt
     1681 tgccggagaa gtcgccctct ccggcagggg agcacaaggg attaccctgt ctgatcagag
     1741 tggacgcggg cgggattggg tatgaggcgg ttcaacggac gtctttggcg tctttactct
     1801 tttccttggc cgcgtctatc tcgcggtctt cgtcgaaccc cccgataggc ctcatgaagg
     1861 tgtaccggaa gggctcctcg acgagcttct tggccaccgt ctcgacgctg tcgctcggaa
     1921 tcgaaaaggg caacgagacg atgaacaggg ccgtaccgac gacggttgcc gccaggctca
     1981 ccggcctgac tatgaggatg tcagccggaa cgggtatatc gggctctttc ccaaaggccg
     2041 gcccggacac cccggacgcg atgacaagcg ctaacgccac gaacaacgtt gctgctgcct
     2101 tcatcgaaaa cctccttaca gtttctttgc agtaaatcta acagagacgg ccgaataaag
     2161 caaaaacgaa atggtcgacg cgccgggcgc tgacgtgccc cggcccggat cctgcggtct
     2221 tgagattcgg ttttgagacc tttgacattt gcttctgttt ctgctattaa gctttgccct
     2281 tttatgcagg gggatagggg ttcgtgatac gcacaaatga cacggaggga ttgtttttca
     2341 aaggcatctc atcgataaga aagggagaat cgttccgccc atgaaagaaa ttgacgtccg
     2401 tgatgaaaac atccgaaaag aaacgctggg tactgtcgat ttttacgact ggttagatac
     2461 tttcgagcat aaattacgcc ccaaggtgga gaaatccctg ccgaacgtca gcggcaaatg
     2521 acggtgtttc cctcagcgga aatgcttttt taggaagtcg aggtctattt cggggtagac
     2581 ggggtactgg gacatgaccg cccgcaccct tcccttcgcc tcggcctcgg ccttcggatc
     2641 gacctcatac ttcgcctggc tctttgcacc gcctgcggtc aacggatttg tgttgtccag
     2701 caccaggcgg atgatcgagg cgatctcctt catctcctct ctgcccatgt tcagtgtcgt
     2761 tacggcgggc gtgcctaccc tcaggccgct cgtgtaccag gggccgttcg ggtcgaaggg
     2821 cagggcgtta cgattcaacg ttatgctgca ctggcgtaac gcgctttccg cctgcctccc
     2881 ggtgatgccg aaggggctca cgtcgataag aaacaggtga ttgtccgttc cacccgtcgc
     2941 caccctcatc cccagacgca ggcattcttc gaccattgcg cgggagtttt cgacaatctt
     3001 tccggcgtag gccttgaact ccggacggtt cgcttcgaca aaagccacgg ccttggctgc
     3061 catcacgtgg ggaagcggcc cgccgagtac gaggggacag cccctatcca cagcatcggc
     3121 gaactccccg ctgcagagaa cgacgccgcc ccggggtccc cggagggtct tgtgcgtcgt
     3181 cgtcgtcacc acctgggcga aggggaacgg gctgtagtcg cccgtaaaaa cgccgcctgc
     3241 caccagtccc gcaaaatggg ccatatccac catcaggacc gcgcccgccc ggtctgctat
     3301 ctcccgcatc ttccgaaagt tgatggcccg tggataggcg ctgtagccgg caagcaggat
     3361 gagcggcctc acctccaggg cctgtttttc tatcgcggcg tagtcgagga gccccgtgtc
     3421 cctgtctacc gtgtaggtga aggcttggaa catccgggcc gagacgttgt tacggtatcc
     3481 gtgggtcagg tgcccgccgg aataataatc catgccgagg agccgctggt tgcccatcct
     3541 cacgcgcaga gattcccact gttgtgccgt caattttgag aggtttgtct cgcccaaatc
     3601 ttcgagggcc ggcatctcaa cccttgcgga gaggatcgcc cagtaggcta ccaggttcgc
     3661 gtcggcacca ctgtgcggct gcacgtaggc gtggtcgcag ccgaagagtt tgcaggcctc
     3721 ggttgcggcc aggctctcca cggcgtccac gttctcacag cccgcgtaga agcggtggcc
     3781 ggggaatccc tcggcgtact tgtccgtcag cagatttccc atggcgaact gggtcgaaag
     3841 ggacgagtag ttctcgctgg cgatcagctt caggttagtt ctttgggcct ctaattcatc
     3901 gatgacggcc ttcgccaccg ggggcgagac gcgggagacg acctccaggt ttgcgaggaa
     3961 ggcaagaaac tctggtttta tctcctcagc ttttttcccg acaaggtatt cgacaacggg
     4021 acttgcgacc ataggggtcc cctttctttg accgattctt cacacggccc aggcgcacga
     4081 ccgtgtgagt agagctcccc gatggttttc catcttgaac gccagttgct caagtaacga
     4141 cgggattaac ggtatcacac gaaaagaaca cgatgggccg gtttttacca gaagttcatc
     4201 tttttgcaag gacaatttac ttccgtccgg cgccgccctg aaggaacttc aagtaatgtc
     4261 cggccgtctc ctccggcctc tccagcatcg gcatgtgccc gcagtccttc atgatgactg
     4321 tctgatggtc cttgatgcgt ttttcgatta tcggcacaca cgacacgtca aggatgttat
     4381 ccctgtcgcc ccagaggatc agcgtcttcg ccttgatgag tggtagataa gactcgagag
     4441 aaataggctt cctgagcatg tctgccatga ccttctcgtt gaaaggcccg tctttgatag
     4501 cccgcgcgac tatggccttt ctcacgggat aggggatcgc cgggaccttc acgaaggcga
     4561 gtctcatcat ccgttcgaag tcgtcgtcgc ttttgatcac cagcgggttg acacccttct
     4621 cgaacatctt catcaattcg cttttcacac cgccctgtat ccctcccggc gcgaggagtg
     4681 ccaagctcaa cacctcctgc ggatgcttgg cggcgtaaac ggccgcgatc gtaccgccca
     4741 ttgaattgcc ggcgacgttc accttcccca gtctcatgac ctggacgaaa cgatgaagac
     4801 gctcgacctg actctcgatg tcgtaacttg cctcctggtg gcgagagctc tctccgaagc
     4861 cggggaagtc agggatgacg acgtgatatt taggggtgag gtactttgcg aaggccgtcc
     4921 agttgtcctt gtcggccgtg taaccatgga gtagaaggat gttttcccct ctaccgccct
     4981 ccagatagac gatacgactg tcgtccacct gtacggaatg ttcggatagg ccggaaagac
     5041 tacggtttac gtactgggcc gcccggaaga ccgtaccggg gaacagaata aagaacagga
     5101 ccagggcgac gattatgaca atcagtacga ccccgagccc cccggctttt cgtcctttca
     5161 tcgcctttcc tccgcacgct cggctgccgg ttgttttaag ggtttttatc ttcgctacac
     5221 atccaggttc tgggcgtaga gggcgttttt gtaaatgaag tccttacggg gttcgacctg
     5281 gtcccccatc aaagtggtga agatatcgtc ggcgatgacg acgtcttcga ccttcacctg
     5341 taagagagtg cgcttctccg gattcatcgt cgtttcccag agctgctccg ggttcatctc
     5401 gccgaggcct ttgtagcgct gcacggtgag ccctttctgg cccagggcat tcacatgctc
     5461 gacgagggcg gtcataccgg cgagttcctt cttctccccg tcttcgtcga ccaccgtgta
     5521 cggcggctcc cccagggcac taacttggtt gaggaggtcc ttgacctcca ggaaccgggg
     5581 ggatttgaat atctcccggt ccaggaccgt ggtgaagacc tgtcctttca tccggtagtc
     5641 gaggatcagc ttgtagccgc tgtgctccgg gtccggctcc gtccgatagt ctacgagcct
     5701 ttcaccgaca gccatcctta ccctcttcgc gaccagcaag agggccgcct cgtcctggaa
     5761 atcctccggc gaaaaggccg ggtccccggc aagtatccgc attacagtcc ggttcttttc
     5821 ctccttctca aaacggtcga ggacggtttc gatccgcatc acacgtttaa ccaactggag
     5881 ca
//
LOCUS       DAOTMX010000079         5876 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_11553_length_5876_cov_4.128844, whole genome shotgun sequence.
ACCESSION   DAOTMX010000079 DAOTMX010000000
VERSION     DAOTMX010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5876
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11553_length_5876_cov_4.128844"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="P5573_03300"
     CDS             complement(<1..220)
                     /locus_tag="P5573_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27123.1"
                     /translation="MKKKRSSSYFISVFLLVFLMTIVGNQAGCSSSSKKETEVIGYPQ
                     SFVELAERVKPAVVNISATKTVRVPGNPF"
     gene            complement(361..1305)
                     /gene="epmA"
                     /locus_tag="P5573_03305"
     CDS             complement(361..1305)
                     /gene="epmA"
                     /locus_tag="P5573_03305"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HRT27124.1"
                     /translation="MSRNDLLTPEDWRIARRRDFLRRRALVVNSVRDFFVGRGYLEVE
                     TPHRIPAPAPECFIEAVPAGDWFLHTSPELCMKRLLAAGHARIFQICRCFRQGELGSR
                     HLPEFTMLEWYRAGADYRELMSECEDLVTTLAQDLDMPGGLVRGGRTIDLRKPWERLT
                     LKEAFERYAPVPLDEALAVGTFDELMAFEVEPRLSMDRPVFIHDFPVSLGALARRKSG
                     NPEVAERFELYIGGLELVNAFSELTDAGEQRERFLKEEAKRRAMGKRPYPVPEKFLLD
                     LAKMPESAGAALGMDRLVMLFTGAEEIGDVVAFRPEEL"
     gene            complement(1292..1855)
                     /gene="efp"
                     /locus_tag="P5573_03310"
     CDS             complement(1292..1855)
                     /gene="efp"
                     /locus_tag="P5573_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HRT27125.1"
                     /translation="MYSAADLRKGLRIKLDGDPYTITEFNFVKPGKGQSLYRCKLRNM
                     ITGAQFERTFREVDTFEPADLQQKAMQYLYKEGDNYVFMDLESYEQVYLTDDQVGDAK
                     NYLIDSLEVEILLFEDRPIGVTLPNSVDLRVEHAEPWAKGDSVSGNYKPVVLETGYRI
                     LVPPFIEEGEKIRVDTRSGEYLTRVKE"
     gene            complement(1975..3201)
                     /locus_tag="P5573_03315"
     CDS             complement(1975..3201)
                     /locus_tag="P5573_03315"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRT27126.1"
                     /translation="MLVLTASVIIEVCLGLILAWPTILTERVTSAHLDFSVQLRWIHA
                     LTLVAFAFSTVAAGKLLDRVDPGLCVRAGGVALGVGLAAAGMAGKISVMWLCFAGIVA
                     GSGAGFAYLVPVILAVRLFPSEKGLASGIVAAGFLSGVIIWSRIAVYLVNVHLLSWSV
                     VHILLGLTVLVCCLAGSFFVTRSDTDRREEEWHALSDNFTRPKYMPGGLGDKLASYTF
                     YAIFFVFFVSAFSGHIASGIIQPYGIESLRARGLENKIAMSVSALAVFLFQLASVVGP
                     PAWGMISDRMDRRWVIAVLCGIQGVVLFLMAYTVGSPQSVILFACLTGFNYGGNLGLF
                     PAVTAENFDDRSLTWVYSLIFLAFIAAGVAGPETTDYFFGEAGTGEASLGKTPLVVLA
                     AACLGVSVLALFFKGR"
     gene            complement(3292..4620)
                     /locus_tag="P5573_03320"
     CDS             complement(3292..4620)
                     /locus_tag="P5573_03320"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HRT27127.1"
                     /translation="MAEQKTFNRWLVVVGALLIQVSLGAVYIYSVFKPALGARFPDWS
                     ATDLALPSQIILAFFALSTIFAGRIQDKIGPKKVATVGGILLGLGLVLSSFAQNLLVF
                     TLCFSVLGGIGIGTAYVCPIATCVKWFPDMRGLITGLAVAGFGAGALVFTPVAKALIA
                     SQGIMAAFLWLGVIFLVAVVLGAQLMVNPPAGYKPAGWNPPASVAGVSSQGSDFTWQE
                     MLKTSQFYLLWLTYFAGCTAGLMIIMNVLNIWQSVSILGLAGTGTTIPKDVFKGITDQ
                     GATAIMVVAILNALGRIVWGKVSDNLGRKNTLAVMFLFSGIVMLALNYMSSFTLYLVG
                     VSCIGFCFGGFLALYPAVTADYFGTKNVGANYGWMFTAYGAGGLFGPWLAPKLMTVVQ
                     KVPYEAVEKGTIVIKQYAAGNFLTSFIISGIMCLIAVAVVWAIKPPVKKA"
     gene            complement(4973..5530)
                     /locus_tag="P5573_03325"
     CDS             complement(4973..5530)
                     /locus_tag="P5573_03325"
                     /inference="COORDINATES: protein motif:HMM:NF013358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HRT27128.1"
                     /translation="MEGKLANVGPLGLMGFGMTTVLLNIHNAGFYPLGSMILAMGLCY
                     GGFAQIIAGILEFKKGNTFGVTAFTSYGLFWCSLVFILVFKELPATPVGYMGWYFFMW
                     GLFTFFMWFGTFGKNRAIQFVFLSLTVLFWLLAIRDWTGSAAIGILAGWEGIVCGLSA
                     IYLAMAEVLNESLGRVVLPIGEIKR"
BASE COUNT         1539 a         1696 c         1407 g         1234 t
ORIGIN      
        1 ggaaggggtt gccggggacc cgaacagtct tggtggcgct tatattcacg acggccggct
       61 tgaccctctc ggcaagctcc acaaaagatt gaggataacc gatgacctcg gtctctttct
      121 tgctggagct cgagcagccg gcttgattgc cgacgatcgt cattagaaac accaaaagaa
      181 agacggagat aaaatagctt gaacttctct tttttttcat atcctttctc ctccttttac
      241 tttcccggtc accatcataa ccgaattccc ctgaaaaaaa gcaaatctta gagtaccgga
      301 gcggatgtct tcaaatcccg ggcccattgt cagaaaaaca gatgggccct atcctcgagt
      361 tcacagttct tcgggcctga aagcaacgac gtcgcctatc tcttccgccc cggtgaaaag
      421 catcaccaac cggtccatac cgagcgccgc tccggcggat tcgggcattt tcgcgaggtc
      481 gagtaagaac ttctccggca ccggataagg cctcttgccc atggcgcgcc gcttcgcctc
      541 ttctttcaga aagcgctccc gctgctctcc ggcatccgtc aattccgaga aggcgttcac
      601 gagctccagg ccgccgatat aaagctcgaa acgttccgcg acttcgggat tgccggactt
      661 gcgcctcgca agggcgccca gcgaaaccgg gaagtcgtga ataaacaccg gcctatccat
      721 gctcaacctt ggctcgacct caaaggccat cagttcgtcg aaagtgccga cggcgagggc
      781 ttcatcgaga ggtaccggcg cgtacctctc gaaggcttct ttcagcgtca accgttccca
      841 gggcttgcgg aggtcgatcg ttcggccccc gcgtacgagg cctcccggca tgtcaaggtc
      901 ctgtgccagg gttgtcacca agtcctcaca ttccgacatc agctctcgat aatccgcccc
      961 cgctcggtac cattcgagca tggtgaattc gggaaggtgc cgactgccga gctcgccctg
     1021 ccggaaacac cggcaaatct ggaatatccg ggcatggccc gccgcgagga gtcttttcat
     1081 gcagagctcc ggtgaggtat ggagaaacca atcaccggca ggaacggctt cgatgaagca
     1141 ttctggtgca ggagctggta tgcggtgggg ggtctctacc tcgaggtacc cccggccgac
     1201 aaagaaatcg cgaacgctgt tgacgaccaa agcccgccgc ctgagaaaat ccctccgcct
     1261 cgctatgcgc caatcttccg gagtcagaag atcattcctt gacacgcgta aggtactcac
     1321 cgctccgggt gtcgacacgg attttctcgc cttcttcgat gaacggcggg accaatatcc
     1381 tgtaccctgt ctccaggacg acgggtttat aatttccgga gacgctatcc cctttggccc
     1441 agggctcggc atgttcgacg cggagatcga ccgaattagg gagcgtgacc ccgatgggcc
     1501 tgtcctcgaa gagaagaatc tccacctcga ggctgtcgat gagataattc ttggcatccc
     1561 ctacctgatc gtcggtgaga taaacctgct cataggactc aaggtccatg aacacataat
     1621 tatctccctc tttgtataag tactgcatcg ccttctgctg gaggtccgcc ggctcgaagg
     1681 tgtcgacctc gcggaaagtg cgctcgaact gcgcccccgt tatcatgttg cggagtttac
     1741 accggtaaag ggattggcct ttcccgggct ttacgaagtt gaactctgtg atcgtgtaag
     1801 ggtcaccgtc cagttttatt cgcagaccct tcctcaaatc ggcggcactg tacattcgtt
     1861 gaagacgctc cttcgataag actttagacg tggagacacg gaaaatattc tttaactacc
     1921 acactttcaa aaaagaaaaa ataagaattt gccgatcaat gctctgcaaa aatatcatct
     1981 gcccttgaaa aacagcgcaa gcacagagac gcccagacaa gcagccgcga ggacaacgag
     2041 cggcgtcttt ccgagcgacg cctcgcctgt tcccgcctcg ccgaaaaaat aatccgtcgt
     2101 ttcaggaccg gcgacccccg cggcaatgaa agcgagaaaa ataaggctgt aaacccaagt
     2161 aaggctgcgg tcgtcaaagt tttcggcggt cacggcggga aagagaccca aatttccccc
     2221 ataattgaac cccgtcaggc aggcaaagag gatgacggac tgtggtgaac ccaccgtgta
     2281 tgccatcaga aacagcacca cgccctgaat tccgcagagt acagcaataa cccatctccg
     2341 gtccatccgg tccgaaatca taccccaagc cggggggcca accacgctcg ccagttgaaa
     2401 gaggaacacg gccaaagcag aaacgctcat ggcaatcttg ttttcaagac ctctcgcccg
     2461 gagggattcg atgccgtaag gctgaattat tccagatgcg atatgccctg aaaaggctga
     2521 aacaaaaaaa acgaagaata ttgcatagaa agtatatgac gctaatttgt ctccaaggcc
     2581 tccgggcatg tacttaggcc tggtaaagtt atcggacagc gcgtgccatt cctcctccct
     2641 tcggtcagta tccgagcgag ttacaaaaaa gcttcccgca aggcaacaca ccaaaacagt
     2701 caatccgaga agtatatgga cgacactcca agataagaga tgtacgttga caaggtaaac
     2761 agctattcta ctccatataa taacgcccga taaaaaaccc gccgccacaa tccccgacgc
     2821 cagccctttt tcggagggaa acagcctgac ggcaaggata acgggaacta aatacgcaaa
     2881 acctgccccg ctcccggcaa caattcctgc gaaacagagc cacatcacgg agatttttcc
     2941 ggccatcccc gccgccgcaa ggccgacgcc gagggccacc cccccagcgc ggacacacag
     3001 cccgggatcg accctgtcaa ggagtttccc cgccgccacg gtggagaacg cgaatgcaac
     3061 cagggtgagg gcatgaatcc atctaagttg aacggagaag tcgaggtgag ctgaagtcac
     3121 cctttcagtc agtatggtcg gccaggcaag gatgagcccc agacatacct ctatgatcac
     3181 agaagccgtc aatactagca tccgggtcga atcagacctg cctgttctat gtgtcataag
     3241 gagtagataa aaagggggca acttctattg ccccctttcc tcgtcgagca gttacgcttt
     3301 cttgaccggt ggttttatag cccagaccac ggcgacggcg atgaggcaca tgataccgga
     3361 gatgatgaac gatgtcaaaa agttgcctgc ggcatactgt ttaatcacga tggttccttt
     3421 ctccaccgct tcatagggaa ctttctgcac aaccgtcata agtttggggg caagccaagg
     3481 gccgaaaagt ccgcctgccc cgtaggccgt gaacatccag ccgtaattag ctccgacgtt
     3541 tttcgtaccg aaataatccg ccgttaccgc cggatagagc gcaagaaatc ctccgaaaca
     3601 aaaaccgata caggacaccc cgaccaaata aagggtaaag gaggacatat aattcagcgc
     3661 aagcatcact ataccgctga ataggaacat gacggcgagt gtgttttttc gtccgaggtt
     3721 gtctgaaacc tttccccaga caatgcggcc cagtgcgttc aatatggcca caaccattat
     3781 agcggtcgcg ccctggtccg tgatgccctt gaagacgtcc ttcggtatcg tcgtacccgt
     3841 acccgccaga ccaaggatgg aaacggactg ccagatgttg agaacattca tgatgatcat
     3901 caacccggcc gtacagccgg cgaaataagt caaccacagc aagtaaaatt gagatgtctt
     3961 cagcatctcc tgccatgtga aatcacttcc ctgtgacgag acgccggcaa ccgatgcagg
     4021 cggattccat cccgccggtt tataaccggc cgggggattt accataagct gggccccaag
     4081 cacgactgct accaggaaaa tcactcccag ccagaggaat gcggccatta taccctgtga
     4141 ggcgatcagc gccttcgcca cgggtgtgaa gaccagggcg ccagctccga atcccgccac
     4201 agcaaggccg gtgatcaggc ctctcatgtc gggaaaccac ttcacacagg tcgcaatagg
     4261 acagacatag gccgtaccga ttccgattcc gccgagaaca ctgaagcaca gggtgaagac
     4321 aagtagattc tgtgcaaaag aagataggac caatcccagg cctagcaata taccccccac
     4381 cgttgcaacc ttcttcggcc ctatcttatc ctgtattctg cccgcgaaga tggtgctgag
     4441 ggcaaaaaag gcaaggatga tctgcgaggg aagggcgagg tccgtagcgc tccaatcggg
     4501 aaatctggcg ccaagggccg gcttaaaaac gctgtaaata taaacagcac cgagacttac
     4561 ctgaatcaag agagcgccga caacaacaag ccacctgtta aacgtcttct gttctgccat
     4621 aaacaatcgt tcctcctttt cgacatgatg attctataaa taattccaac gattttccgc
     4681 tctgaatcgg caggcaaaat ttttcgttcc ttatccaaat tcaaaccgat tgtcaaacaa
     4741 aaagcgcggg cgacaaaaag agggaaaatc aaaaacggag ggaaaatcaa gaaggtccag
     4801 tcagttcatc cggcgtgttg acatacgccc tatcgtatac cagccctatc ctacttcgat
     4861 gtccgaaaaa gctctctgcc gatatgccga ctttctgttt tttgcgagcg tctcgtcatc
     4921 ggttaaaaag aaggggggat ttcccccctt ctttcgatcg gtaaggccgg tactatcttt
     4981 ttatctcacc tatcggaagc acgacccttc ccagggactc gttcaacact tccgccatcg
     5041 ccaaatatat agccgaaagt ccacacacga taccttccca acctgccagt atccctatcg
     5101 ctgcactgcc tgtccaatcc cttatggcga ggagccagaa cagtaccgta aggcttagga
     5161 acacgaactg tatcgccctg tttttaccga aggtgccgaa ccacatgaaa aaagtaaaaa
     5221 gtccccacat aaagaagtac caccccatat atcccaccgg ggtagccggt aactccttaa
     5281 aaaccaggat aaatacaaga ctgcaccaga aaagcccgta cgaagtaaag gcggtcacgc
     5341 cgaaggtatt gccctttttg aactcaagga tgcctgcgat tatctgtgca aaacccccat
     5401 agcacaaccc catcgcaagt atcatcgacc ccaatgggta aaaccccgcg ttgtgaatgt
     5461 tcagaagcac cgttgtcatc ccgaatccca taagaccaag cggcccgaca ttagcaagct
     5521 ttccttccat atgtcctccc tttcatgatt aaattaacgc acttaatgta cccactaccc
     5581 acatatacgc agccactcat attggatttg ccaaccttat ggcggctgct ttctaacagg
     5641 gaagtatgat ttacgtcaag aaaaaaccgg ccccccactc tatcgacata gactgttatt
     5701 gcaacactgt ttatcgccgt gtaatcagga tagtccagtg aggtcatccg gcgtgtttat
     5761 gttaaggaac aggttcccgg ctgcgccaaa tttattgatc tcgtcttccc ggatgacgag
     5821 ggtacggcgg cggttgaaca gttcgctcac tttcaatgta cccctctcga tcatcc
//
LOCUS       DAOTMX010000080         5870 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_11567_length_5870_cov_3.664660, whole genome shotgun sequence.
ACCESSION   DAOTMX010000080 DAOTMX010000000
VERSION     DAOTMX010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5870
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11567_length_5870_cov_3.664660"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="P5573_03330"
     CDS             complement(<1..530)
                     /locus_tag="P5573_03330"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRT27129.1"
                     /translation="MADILLQEKTDEGILILTLNRPDAMNCFNFDLLAALDDVIREAN
                     FDMGIRCIIITGAKAGDDPKKWSFSTGADLKERRTLTPDQVRRFIFTIRNCFTAVEQV
                     RVPVIAAINGFAFGGGTELALACDLRIASSNVLMGLTETSLAIIPGAGGTQRLTRIIG
                     IAKAKDQTFRELFEER"
     gene            complement(573..2246)
                     /locus_tag="P5573_03335"
     CDS             complement(573..2246)
                     /locus_tag="P5573_03335"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRT27130.1"
                     /translation="MAEEKKRLTRAERLAMEREDNLKHWEEQDALLFKHREEAYNIGG
                     PEQVTRLAKQGKKPMRDLINMLIDPGTEFYELGIDAGYNIGKVKLYGGDVYESEKRGH
                     IPGGGLVTGVGVIHGKDCMIFANENRYAAGTYFPITLKKHMRAQAIAERLRLPCVYIA
                     DSGGINLPLQLGCFADDGHFGSMFYNMCRMSALGIKQYTLSTGGNTAGGAYIVYLASE
                     SIMIEKLAFAFLAGPPMVKSAIGETVSAEDLGGAYVHTQHSGGCDHFVRTQEEGIKKV
                     RDIIEFEPQQSLYCELKKPAEPKFDYKEMMRCTPINTYHAINIKDTLKCLADDSYFHE
                     YKPTYGLYRGETIVAGKMWMKGMPVGVIASSGNGVIYIEAARKATEWMIRCGNSNLPV
                     LFLQGSPGYMVGKSEEWGGIGKYGSDMVRTCSVLNVPKIQYVIGPDHGAANYGMCGRA
                     FRPVFCFTNMRGRTTVMSGETAGFIVETVRRKNIIDKGQQVDEEEMAKFRQMMRDRYD
                     AEGHPFLTGSLLFHDGVIAFSEARNKLARALELCYRVPAGHTDWGDLKV"
     gene            2507..3328
                     /gene="mazG"
                     /locus_tag="P5573_03340"
     CDS             2507..3328
                     /gene="mazG"
                     /locus_tag="P5573_03340"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HRT27131.1"
                     /translation="MGIKEEGAEGEFEVKHLFGSLVGIVARLRSPEGCPWDRKQTISD
                     AARYLLEETYEVLDAVDNGDPDGLKEELGDLLFQILFISRLAEEEKKFDMASVIKAVS
                     EKMVRRHPHVFGDKKVLSVEDVKRNWDDIKRHVENKEPGQRSCIDGIPLSMPALMRAQ
                     KIAEKAARVGFDWEDVRGVLQKVDEETEELKRAIAAGSREQVEEELGDLLFVLVNLGR
                     FSGVDAEAALRASIRKFEGRFSFIERKLASKGRSPAEASLAEMDALWNEAKKSDE"
     gene            3325..4368
                     /gene="queA"
                     /locus_tag="P5573_03345"
     CDS             3325..4368
                     /gene="queA"
                     /locus_tag="P5573_03345"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HRT27132.1"
                     /translation="MKTKLEEFDYHLPNDLIAQRPAESRDFSRMMVLDRKSGECRDCC
                     FAQIVDFLGKGDCLVINDSRVIPARLLAAKETGGRVEMLLLRKVDGSPHLWEVLLKPG
                     GRVSPGMRFRLGEDAWVEAVERTGEKKWVVDFHLKIEFTDFVERYGRVPLPPYIRRKR
                     NSEEPLELLDRMRYQTVYARVPGSVAAPTAGLHFSEDLMGRIAAAGVAIAPVTLHVGY
                     GTFRPIESDFVEDHVMEEEYFDLSDESAEKINRAVRVVAVGTTSTRVLESVSDEKGRV
                     KPTGGYTGLFIRPGYRFKRVNALLTNFHLPKSSLYVLASAFAGNDLIRKAYAKAIGDH
                     YRFYSYGDCMLIL"
     gene            4452..5588
                     /gene="tgt"
                     /locus_tag="P5573_03350"
     CDS             4452..5588
                     /gene="tgt"
                     /locus_tag="P5573_03350"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HRT27133.1"
                     /translation="MSFSFEVLRKDTASGARLGKMTTLHGDVETPAFMPVGTQGTVKG
                     LTPEQLERLGADIVLANTYHLYLRPGHESVRNLGGLHRLMGWSHPILTDSGGFQVYSL
                     GPLRKVTEEGVFFRSHIDGSQHFIDPEKAVAVQEFLGADIMMCLDECIPYPADYSYSE
                     KSLKLTIRWAERCKKAQSGTGQALFGILQGGMYPDLRIRGIEELVRIGFDGYAFGGLS
                     VGEPKDLMMAVIEETAPLFPVDKPRYLMGVGTPEDIVDCVHRGVDMFDCVLPTRCARN
                     GMLFTNNGKVVIKNARYRDDAEPLDGSCDCYTCRNFSRAYLRHLYVAREILAIVLNTI
                     HNIRHYMNLMEEIRRAIAAGDFYGFREHYKKRQAVVCGESVREG"
     gene            5642..>5870
                     /gene="yajC"
                     /locus_tag="P5573_03355"
     CDS             5642..>5870
                     /gene="yajC"
                     /locus_tag="P5573_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HRT27134.1"
                     /translation="MINLAYAMGAPGGGGGSQGGDIQFIIMMAIVFLIFYFLLIRPQQ
                     KKQKELREMINKVKHGDVVVTTGGIYGKVMSV"
BASE COUNT         1347 a         1421 c         1716 g         1386 t
ORIGIN      
        1 tgacgttctt caaacagttc gcggaaggtt tggtccttgg ccttggcaat gccgatgatc
       61 cgggtcaacc tctgtgttcc gccagcgccg gggatgattg cgagactggt ttccgtgagc
      121 cccatcagta cattcgacga agctatgcgg aggtcgcagg caagcgccaa ctctgtcccg
      181 cctccgaagg caaagccgtt gatcgcggcg atgacgggaa ccctaacctg ctcgacggcg
      241 gtgaagcagt tccggatcgt gaagatgaaa cgtcggacct ggtcaggtgt tagcgttctt
      301 cgctctttta ggtcggcgcc cgtggagaaa gaccatttct tcgggtcgtc tcctgctttg
      361 gcaccagtta tgatgatgca acgaataccc atatcaaaat tggcttcccg gatgacatcg
      421 tcaagcgcgg caagcaggtc gaaattgaaa caattcatgg catccggtcg attcaaagtt
      481 agaatcagga tgccctcatc tgttttttct tgaagcagta tatcagccat aaattttcct
      541 cctcttattt agctctttac ctctcgaacc ggttaaacct tgaggtctcc ccaatcagtg
      601 tgtccagcgg gcacacggta acagagttcc agagcccttg caagcttgtt tctagcttca
      661 ctgaaggcga tgacgccgtc gtgaaacagg agcgagcctg tgaggaaggg atgaccctcg
      721 gcatcatacc ggtctctcat catctggcgg aattttgcca tttcctcctc gtcgacctgt
      781 tgacccttat cgatgatatt ctttctcctt accgtctcga cgatgaaccc tgccgtctca
      841 ccggacatga ccgtcgtgcg acccctcatg ttggtgaagc agaaaacggg tctgaaggcc
      901 ctgccgcaca taccgtagtt ggccgccccg tgatcaggac cgattacgta ctgaatcttg
      961 ggcacgttga ggacgctgca agtgcggacc atgtcggacc cgtatttacc gatacctccc
     1021 cattcctcag acttgccgac catgtagccg ggggagccct ggaggaagag gacaggtagg
     1081 ttcgagttac cacagcgaat catccactcc gtcgccttcc tggcggcctc gatgtatatg
     1141 acgccgttgc cgctcgatgc gatgacgccg accggcatac ccttcatcca cattttgccg
     1201 gccacgatcg tttcgcccct gtagaggccg taggtcggtt tgtattcatg gaaatagctg
     1261 tcgtcggcga ggcacttcag cgtgtctttt atgttgatgg catggtaggt gttgatcggc
     1321 gtgcaccgca tcatttcttt gtaatcgaac tttggctccg cgggcttctt gagttcgcag
     1381 tagaggctct gttgcggctc gaactcgatg atatcacgaa cttttttaat accctcctcc
     1441 tgggttctca cgaagtggtc gcagcctccc gagtgctggg tgtggacata agcgccgccg
     1501 aggtcttcgg cgctgaccgt ttccccgatc gccgatttga ccatgggagg accggcaagg
     1561 aatgcgaaag ccagtttctc gatcatgatg gattccgagg cgagataaac gatgtacgca
     1621 ccgcctgcgg tgttgcctcc tgtggacagc gtgtactgtt tgatgcccag ggcggacata
     1681 cggcacatgt tgtagaacat gcttccgaag tgcccgtcgt cggcaaaaca gcccaactgg
     1741 aggggtagat tgattcctcc cgagtcggct atgtagacgc agggcagtct caggcgctcg
     1801 gcgatggcct gagctctcat gtgctttttg agggtgatgg ggaaataggt tcctgccgca
     1861 taacgatttt cgttggcgaa aatcatgcaa tcttttccgt gaatgacgcc cacgccggtc
     1921 acaagtcctc caccggggat atgtccccgt ttctccgact cgtaaacatc gccaccgtag
     1981 agcttgactt tcccgatgtt gtatcctgcg tcgattccga gttcgtaaaa ctccgtgcct
     2041 ggatcgatga gcatatttat gaggtcgcgc atgggcttct tgccctgctt ggcgaggcgt
     2101 gtgacctgct cgggaccgcc gatattgtaa gcttcttcac gatgtttgaa cagaagggca
     2161 tcctgctctt cccaatgctt aaggttgtcc tccctctcca ttgcgagcct ttcggcccgc
     2221 gtcaagcgct tcttctcttc tgccatggat atcgtcctcc tctctataaa aaaccaggtt
     2281 acgttaagga atgcaaaatc tcttcagaaa agatgacttt gcaactgaaa ccgtatggtg
     2341 tgcaacaaaa aggccggtcg ttcaaagcgc gaacgtctta tcaccgcgac aaaataaaat
     2401 caagccaaaa cacccatcag accccggcct ttcaggtaaa tcctaatttt gacaccttat
     2461 actaatcgtg ctaaaaatag caactttttt gcgaggaaaa gataggatgg gaatcaaaga
     2521 ggaaggcgca gaaggtgagt ttgaggtcaa gcatctgttc gggtcgcttg tgggcatcgt
     2581 tgcacgactg agatcacccg aaggttgccc gtgggacagg aagcagacca tatccgatgc
     2641 cgcaaggtac ctcctggaag agacatacga agtgctcgac gccgttgata acggagaccc
     2701 ggatggcctg aaggaagaat tgggggacct gctctttcag attctgttca tctcccggct
     2761 tgccgaagag gaaaagaaat tcgatatggc atcggtgata aaggccgttt cggagaagat
     2821 ggtacggagg cacccccatg tgttcggcga taaaaaggtg ttgagtgtcg aggatgtaaa
     2881 gagaaattgg gatgatatta aaaggcacgt ggaaaacaaa gagccaggac aacggtcgtg
     2941 cattgacgga atccccctgt ctatgcccgc cctaatgagg gctcagaaaa tcgcggaaaa
     3001 ggccgcccgg gtgggttttg attgggaaga tgtccggggt gtgttacaga aagtcgacga
     3061 agaaacggag gagttgaaaa gggccattgc cgccggcagt cgggaacagg tcgaggagga
     3121 gttaggcgac cttctttttg tgctcgttaa cctggggcgg ttttccggtg tagacgctga
     3181 ggcggctttg agggcatcga taagaaagtt tgaggggcgg ttttccttca tcgagcgaaa
     3241 attggcgtcg aaggggaggt cgcctgcgga ggcctcgctt gcagagatgg atgccctttg
     3301 gaatgaagca aagaaaagcg acgaatgaaa acaaaattgg aagagttcga ctatcacctg
     3361 ccgaatgacc ttatagcaca gagacctgcc gaatccaggg atttttcgcg gatgatggtt
     3421 ctggacagga aaagcggaga atgtcgggac tgttgttttg cgcagatagt cgatttcctg
     3481 gggaagggcg actgcctggt cataaacgac tcgcgagtca ttcctgccag gctgttggcg
     3541 gctaaggaaa cgggcggtcg tgtggagatg ctcttgctca ggaaggttga cggttcgccg
     3601 cacctatggg aggtattgtt gaagcctggc ggtcgggttt cgcctggtat gaggtttcgg
     3661 ctcggtgaag atgcctgggt ggaggcggta gaacggacag gtgaaaaaaa atgggtggtt
     3721 gattttcatt tgaagataga gtttaccgat ttcgtggaaa ggtatggccg ggtgcctcta
     3781 ccgccgtaca tccgcagaaa gagaaattcc gaagagccct tggaactcct ggacaggatg
     3841 aggtaccaga ccgtatacgc aagggtgccc gggtctgttg cggcaccgac cgcggggctt
     3901 catttttccg aagacctgat gggaaggatc gccgccgcgg gcgttgcgat tgcacccgta
     3961 acgctccatg taggttacgg aacgttccgg ccgattgagt cggattttgt cgaggaccat
     4021 gtcatggaag aagaatactt tgacctgagt gatgagtccg ccgaaaagat caacagggcc
     4081 gttcgggtgg tagccgtcgg gactacgtcg acaagggtac tggaatccgt atcggatgag
     4141 aagggtaggg tgaagccgac aggcgggtat acgggtcttt tcatccgacc gggctatcgt
     4201 ttcaaacgag taaacgcctt attgaccaat tttcacctgc caaagtcatc gctctacgtg
     4261 ctcgccagtg cctttgccgg caatgacctc attcgtaaag cctacgcaaa ggccatcggg
     4321 gaccactata ggttctacag ctacggagac tgtatgctga tactttaagg gtgtcgagta
     4381 tcgagcgtca agctttcgga ataacgtctt tttttaatgt tcgatgccca tagtggatgc
     4441 cgattgatat catgtcgttt tccttcgagg ttttgagaaa agataccgca agcggggcca
     4501 gactgggcaa gatgacgact ctccacggag atgtggagac gcccgccttt atgcctgtcg
     4561 gaacacaggg gacggttaag ggattgacgc cggaacagct tgagcgtctt ggtgcggaca
     4621 tcgtccttgc caatacatac cacctttatc tgcgtccggg ccatgaatcg gtccggaacc
     4681 tggggggact acaccgcctg atgggctgga gccaccccat cttgacggac agcggggggt
     4741 tccaggttta cagtcttggg ccgctcagga aggttacgga ggagggtgta ttctttcgat
     4801 ctcacataga cgggtcgcag catttcatcg accccgaaaa ggcggtcgcc gttcaggagt
     4861 ttctgggcgc cgacatcatg atgtgccttg acgagtgtat tccctatccc gcagattatt
     4921 cctattcgga aaagtcgttg aaactgacca ttagatgggc ggaaaggtgt aaaaaagcgc
     4981 agtcgggtac agggcaggcc ctgttcggga tactgcaagg agggatgtac cccgacttga
     5041 ggataagggg tatagaggaa ctggtgcgga taggtttcga cggttatgcc ttcggcggtt
     5101 tgagcgtggg ggaaccgaaa gacctgatga tggccgtcat agaagaaaca gcccctcttt
     5161 tcccggtaga caaacccagg tatctcatgg gcgtcgggac cccggaggat attgtggatt
     5221 gcgtccacag gggcgtagac atgttcgact gcgtgctgcc cactcggtgc gcaaggaatg
     5281 gaatgttgtt tacaaacaac ggaaaggttg ttataaaaaa cgcccgttat cgtgatgatg
     5341 cagagccgct tgacggctcg tgtgactgct acacgtgcag aaatttctca cgggcgtatt
     5401 tgcgccacct ctatgtggcg agagaaattc tggccatcgt cctgaacacg attcacaaca
     5461 tccgtcatta tatgaatctg atggaggaga tcaggcgggc gatcgcagcc ggggatttct
     5521 atgggttccg cgagcattac aaaaagagac aggcggttgt ttgcggcgaa tcggtgcggg
     5581 aggggtgacg cgatcaccat ttatataatt aatagagtat aggcggtagg aggtttcaag
     5641 catgataaat ctcgcgtacg cgatgggagc gccgggggga ggcgggggca gccagggtgg
     5701 cgacatccag ttcatcatca tgatggcgat cgtctttctg atcttctact ttctcctcat
     5761 caggccccag cagaagaagc aaaaagagtt gagagagatg atcaacaagg tgaagcacgg
     5821 agatgtcgtt gttaccaccg gcggtatcta tgggaaggtc atgagtgtgg
//
LOCUS       DAOTMX010000081         5833 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_11652_length_5833_cov_4.071478, whole genome shotgun sequence.
ACCESSION   DAOTMX010000081 DAOTMX010000000
VERSION     DAOTMX010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5833
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11652_length_5833_cov_4.071478"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="P5573_03360"
     CDS             <1..487
                     /locus_tag="P5573_03360"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF013845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA dimethylallyltransferase"
                     /protein_id="HRT27135.1"
                     /translation="RIHPNDAVRVMRALEYIETSGEPLSLRQRNHRFADRPYDCLKIG
                     MTDERAFLYRKIDSRCDRMVKNGLVGEVEGLLARGFAEDLRPMQSLGYRHIVRHLTEG
                     LPLAEALSLMKQDTRNYAKRQMTWFRADREVRWFGSADWDGIRKEVEAFLGSMVAVEI
                     S"
     gene            599..1774
                     /locus_tag="P5573_03365"
     CDS             599..1774
                     /locus_tag="P5573_03365"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HRT27136.1"
                     /translation="MNHLLKHWHMRYLLFAAVIGALLISCGPKVRVPTSQLDTAEHHV
                     ATGMNLLKEAKYVDAQREFDLAIQLAPKFSMAHTGLGLAMAYQGDLKGAFDAVDRGWK
                     YAKKDDEKVFAQVGYIRLYTMDRANQKKWLEKARNAFETAVKIDPKSSAAYYYMGVAY
                     KYGLEFNQAGQMFTKVLDLKADYLKEAEIEWNLVQKIQRAMPGTVTGKKIAIVESISR
                     ADAAALFMEELRIDILYQKRTPKTFDTSFKDPEKAKARKDSKITAADIANHPLKADIE
                     GILNLGVRGLENYPDNCFHPDELITRGQYAMMIEDILIKVTGDEKLATKFIGTTSPFP
                     DLRPDLPYFNAVMVVTSRGIMEPKDFTTGEFAPLAPVSGADALLIIKKIKEELRFDR"
     gene            1923..4595
                     /locus_tag="P5573_03370"
     CDS             1923..4595
                     /locus_tag="P5573_03370"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HRT27137.1"
                     /translation="MEDNTLSRVYDPHELEVRWYQYWLDHDYFRAEEESDKEPFCIVI
                     PPPNVTGMLHMGHALNNTLQDIMTRLRRMQGYNTLWMPGTDHAGIATQNVVEQELAKQ
                     GLTRHDLGREKFIEKVWEWKERYGNVITNQLKRLGCSCDWSRERFTMDEGLSRAVRQV
                     FVMLYRDGLIYQGDYIVNWCPRCHTAISDLEVEFKEEQGFLWHIRYPYAEGEGEIIVA
                     TTRPETMLGDTAVAVNPNDKRYADVIGKYVVLPLVNKKIPVIADEYVTMEFGTGAVKI
                     TPACDAADFAMAERHNLEIIRIMDGSAVINENGGPYQGQDRYTARKNIVGDLEKGGYL
                     VKTEPYKHNVSQCYRCKTDIEPAVSKQWFVKVKPLAKQAIAAVIKGKTRLIPKMWEGT
                     YFEWMDNIRDWCISRQIWWGHRIPVWYCECGEVIVDAKDPAKCPKCKGTALKQEEDVL
                     DTWFSSALWPFSTLGWPEKTKDLKTFYPTSLLITGFDILFFWVARMMMMGLYVMKDVP
                     FRDVYLHALVRDERGDKMSKSKGNIIDPLDMIEKYGTDAFRFTLAAFTAQGRDIRMSE
                     ERIEGYRNFVNKTWNAARFALMNLQDYVPAGEPKREDFSLCDLWILHRLNRTVADVTR
                     YLDEYRFNDAAGALYHFIWHELCDWYLELIKEVLYGKDNHAGRYAAQHTLAAVFRSTM
                     KLLHPFMPFITEEIWHRLTKDGTSVVVSEFPKADDKLEAPEAAARMELVMEVISSIRN
                     IRGEMGIQPAKKVKVLLSAPDHATVGLLKSCSGYIFTMARLESLVVEEQAAEPSGVAT
                     AVAGPVKVFVFLEGMVDVEGEKERLEKEILKLTKEFQGIQRKLANSDFRQKAAKEAIE
                     KEERKFQETQAKLNVLQSALERLKNVISPARIKE"
     gene            4743..5189
                     /locus_tag="P5573_03375"
     CDS             4743..5189
                     /locus_tag="P5573_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27138.1"
                     /translation="MEVQRDFRDLLALFNAHKVDYLIVGAYALAYHGAPRYTGDMDIL
                     VCPDGENAHRILKVLGEFGFGSVGLTADDFTVPDKAIQLGVAPVRIDIVTSITGVSWE
                     EAVAGRVQGSYGDIVVYFIGREEFIRNKQALGRKKDQADLEALGIG"
     gene            5186..>5833
                     /gene="nadC"
                     /locus_tag="P5573_03380"
     CDS             5186..>5833
                     /gene="nadC"
                     /locus_tag="P5573_03380"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HRT27139.1"
                     /translation="MKTEVRRIIETALREDIGPGDVTTGAVLTGLEVGKARAVAKTEL
                     VVAGLDVFKEVFITLDQEVRFVAKKKDGQKVRRGAVVAEVSGRLKSILQAERVALNIF
                     QRMCGVATITRRYTDLVKGTEAKILDTRKTAPGLRILDKYAVRVGGGVNHRFALYDGV
                     LIKNNHIDAVGGIKAALTRAKAKVGPLLKVEVEVRDFSEVKEAVAAGADMIMLDNM"
BASE COUNT         1483 a         1415 c         1767 g         1168 t
ORIGIN      
        1 gcgtatccat cctaacgacg cggtcagggt catgagggcc ctcgagtaca tcgaaacgag
       61 cggtgagccg ctttccctga gacagaggaa tcaccggttc gccgacaggc cgtacgattg
      121 tctgaagata gggatgacgg acgaaagggc gtttctttat cgtaaaattg acagtcgttg
      181 cgacaggatg gtaaaaaatg gccttgtcgg cgaggtcgaa gggctcctcg cccggggttt
      241 cgctgaagac ctgaggccca tgcagtcgct cggatacagg cacatcgtcc gtcatctgac
      301 ggagggactc ccattagccg aagcgctttc cctgatgaaa caggatacca ggaactacgc
      361 caagagacag atgacctggt ttcgtgccga cagggaggtc cgctggttcg gctctgcgga
      421 ttgggacgga atcaggaagg aagtcgaggc cttcctcggc agcatggtag ccgtcgaaat
      481 ttcttgactt gtgtatcgta taaatatata gaatagcgtg ttttttgagc cgatcgggag
      541 aggcgacgcg tgcccggtgg gattcattga agtcaaccgt tcacgaaagg agaaagtcat
      601 gaatcacctt ttgaagcact ggcacatgag atacctgctt ttcgctgcgg tcattggggc
      661 tttgctcata tcctgtgggc ctaaagtaag ggttccaacc agccagctcg acaccgccga
      721 gcatcatgtc gcaacaggga tgaatctgct gaaagaagcc aagtatgtgg atgcacaaag
      781 agagttcgac cttgccatcc aactggcacc gaagttctct atggctcata cgggcctagg
      841 gctcgccatg gcctaccagg gtgatttaaa aggggccttc gatgccgtgg acaggggatg
      901 gaagtacgcg aagaaagacg atgaaaaggt ctttgcccag gtgggataca taaggttgta
      961 taccatggac agggctaacc agaagaagtg gctcgaaaag gccaggaatg cctttgaaac
     1021 agcggttaag atcgacccga agtcttctgc ggcctactat tacatgggag tggcttataa
     1081 atatggcctc gaattcaacc aagcgggaca gatgttcacc aaggtgcttg acctcaaggc
     1141 ggattacctt aaagaagcag aaatcgagtg gaatctcgtc cagaagattc agcgggccat
     1201 gccggggact gtgacgggca agaagatcgc catcgtggag agcatttcac gggcagacgc
     1261 cgccgccctg ttcatggagg aattgaggat cgacatcctc taccagaaga gaacgcccaa
     1321 gacattcgat acttccttca aagacccgga gaaggccaag gcccggaagg acagcaagat
     1381 tacggcggcg gatatcgcga atcatccgct gaaggccgac atcgagggta tcctgaacct
     1441 cggtgtgcgc ggcctcgaaa actaccccga caactgtttt catcccgacg agttgatcac
     1501 ccgggggcag tatgcgatga tgatcgaaga tatcctgatc aaggtgacgg gcgacgaaaa
     1561 gctggcgaca aaattcatcg ggaccacgtc gcccttccct gacctgaggc ctgatttgcc
     1621 gtatttcaac gccgtgatgg tcgtgacgtc caggggcatt atggagccga aagatttcac
     1681 gacgggagag tttgccccgc tcgcccctgt ttccggtgcc gacgccctcc tgatcatcaa
     1741 gaagataaag gaagagctca gatttgacag atagagaaat ctactggtcg aagccggtgc
     1801 ccttagggat tgtttgatgt ataaagaggc aggggagcaa aagggtctcc tgccttaatg
     1861 tttcggcatg ccgtgttcca agccgacggg aagaacacca ttcaccaaga cagggagagg
     1921 acatggaaga taacacactg agcagggtgt acgaccccca tgagctggaa gtcagatggt
     1981 accaatattg gcttgaccat gattatttcc gcgcagagga agagagcgac aaagagccgt
     2041 tctgcatcgt cataccccct ccgaatgtga ccggtatgct ccacatgggg cacgccctga
     2101 acaataccct gcaggacatc atgacgcgct tgaggcgcat gcagggatac aacactctct
     2161 ggatgcccgg gacagaccac gccggaatcg ccacgcagaa cgtcgtcgaa caggagttgg
     2221 cgaagcaggg attgacccgc catgacctcg gccgggaaaa attcatcgag aaggtctggg
     2281 agtggaagga aaggtacggc aacgtcatca cgaaccagtt gaagcgtctc ggctgctctt
     2341 gtgactggtc gcgagagcga ttcaccatgg acgaagggtt gtcgagggcg gttcgccaag
     2401 tcttcgtcat gctctaccgc gatgggttga tctaccaggg agattacata gtcaactggt
     2461 gtccgcgctg ccacacggcc atatcggacc tcgaggtcga gtttaaggaa gaacagggtt
     2521 ttctctggca tatccggtat ccctatgccg aaggagaagg ggagatcatc gttgcgacga
     2581 cgaggccgga gaccatgctc ggcgacacgg cggtcgccgt gaacccgaac gacaagcgct
     2641 atgcggacgt gatcggcaag tacgttgtcc tgccgcttgt gaacaagaag ataccagtaa
     2701 tagccgatga gtacgtgacg atggaattcg gtacgggcgc ggtgaagatc acccccgcgt
     2761 gcgacgccgc ggacttcgcc atggccgagc gtcacaacct cgagatcatc cgcatcatgg
     2821 acggcagcgc cgtgatcaac gaaaacggag ggccctatca gggccaggac cgttatacgg
     2881 cacggaagaa catcgtcggc gacctggaaa aaggcggtta cttggtgaag accgagccgt
     2941 acaagcataa cgtcagtcaa tgttaccggt gtaagacgga catcgagccc gccgtgtcca
     3001 aacaatggtt tgtgaaggtc aaacccctcg ccaaacaggc aatcgcggca gtgatcaaag
     3061 gtaaaacgcg gctcatacca aagatgtggg aagggacata cttcgaatgg atggacaaca
     3121 tccgagattg gtgcatctcg aggcagatat ggtgggggca caggatcccg gtctggtact
     3181 gcgagtgcgg tgaagtcatc gtcgacgcga aagaccctgc caagtgccca aagtgcaaag
     3241 ggaccgcctt gaagcaggaa gaggacgtgt tggatacgtg gttcagctct gcgctttggc
     3301 ctttctcgac gctcggctgg cccgaaaaga cgaaggacct gaagacattt tatcccacgt
     3361 cgctgctgat caccggcttc gatatcctct tcttctgggt cgcgcggatg atgatgatgg
     3421 gcctttacgt gatgaaggac gtacccttcc gcgacgtcta cctccatgcc ctggtcaggg
     3481 acgagcgcgg cgacaagatg agcaagtcga agggcaacat catcgacccc ttggacatga
     3541 tcgagaagta cggcaccgac gccttccgct tcaccctcgc ggcctttacg gcccagggac
     3601 gggatatacg gatgtcggag gagcgcatcg agggataccg gaattttgtg aacaagactt
     3661 ggaatgcagc caggtttgcg ctgatgaacc tgcaagatta tgtcccggcg ggggaaccga
     3721 aaagagaaga tttttcgctc tgcgacctct ggattcttca ccgcctcaac cggacggtcg
     3781 ccgacgtgac aagatatctt gatgagtacc ggttcaacga cgccgccggt gcgctttacc
     3841 atttcatctg gcatgaactg tgcgactggt atctcgagct gataaaggag gtcctctacg
     3901 gaaaagacaa ccatgccggg cggtacgcgg cccagcacac actcgccgct gtcttcagat
     3961 cgaccatgaa actgctccat cccttcatgc ccttcatcac ggaagagata tggcatagat
     4021 tgacgaaaga cgggacgtcc gtcgtcgtga gcgagtttcc gaaggcggat gataagttag
     4081 aggccccgga ggccgcggcg cggatggagc tcgtgatgga ggttatcagc agcataagga
     4141 acatccgggg ggagatgggc atccagccgg cgaagaaggt gaaggtactg ctgtcggcgc
     4201 ccgaccacgc gaccgtcggc ctgttgaaaa gctgttccgg atatatattt accatggccc
     4261 ggctcgaatc ccttgtcgtg gaagaacagg cggcggaacc gagcggtgtt gccactgccg
     4321 ttgccggtcc cgtcaaggtc tttgtttttc tggaaggcat ggtcgatgtc gagggagaga
     4381 aagagaggct cgaaaaagag atcctgaaac tcacgaaaga gttccagggt attcagagga
     4441 aactggcgaa ctccgatttc agacagaagg ccgcgaaaga ggcgatcgaa aaggaagaga
     4501 ggaaatttca ggaaacacag gcgaagttga acgtgctgca gtcggctttg gagagattga
     4561 aaaatgtaat ttcgcccgcg agaatcaaag aataaggagc gccttcgata acgtgataga
     4621 aaaggtcgtt aagaaagcga atttacggga tttttcggag gtaaaggaaa acctttctta
     4681 ctggctgtca aaagacccgg aggagcgtgt ggaagccgtc gagtacttaa ggaggacgct
     4741 tcatggaagt tcagcgagac ttcagagatt tgctcgcgtt attcaacgct cacaaggttg
     4801 attatcttat agtgggcgca tatgccctcg cgtaccacgg agctccacgt tatacaggcg
     4861 acatggatat tcttgtgtgc cctgacggag agaatgccca ccggatttta aaagtgcttg
     4921 gtgaatttgg ttttggttct gttggtctga cggcagacga tttcacggtt cctgataaag
     4981 cgatacaact tggcgtggcg ccggtccgca tagatattgt cacttccatc accggagtat
     5041 cttgggagga agcggttgct ggtcgagtac aaggaagtta tggcgatatt gtagtgtact
     5101 tcatcggaag ggaagagttt atccgcaaca aacaagcatt gggtagaaag aaggatcaag
     5161 cggatctcga agctctcggc ataggatgaa aacagaagta agacggataa tcgaaacggc
     5221 gctccgtgag gacatcggac cgggggatgt gacgaccggg gctgttctga cggggctcga
     5281 ggtgggcaag gcccgtgccg tcgcgaagac ggaactcgtc gtagccgggt tggatgtttt
     5341 taaagaagta tttataacgt tggaccaaga ggttcgcttt gtcgcgaaga agaaagacgg
     5401 ccaaaaagta aggagaggag ccgtggtcgc ggaggtgtcg gggaggctta agagcatcct
     5461 ccaggcggaa cgcgttgccc tgaacatttt tcagaggatg tgcggggttg cgacgatcac
     5521 ccgccggtac accgaccttg tgaagggaac ggaggcgaaa attctggaca cacgaaaaac
     5581 ggctccggga ttgcgtatcc tcgacaaata tgccgtgagg gtaggcggcg gcgtcaatca
     5641 tcggttcgcc ctttacgatg gagtcctgat caaaaacaac cacatcgatg ccgtcggcgg
     5701 gatcaaggct gcgttaacga gggccaaggc gaaggtagga ccgttgctga aggtcgaggt
     5761 agaggtcagg gacttcagtg aggttaaaga ggccgtcgcc gccggtgccg atatgatcat
     5821 gctcgacaac atg
//
LOCUS       DAOTMX010000082         5825 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_11675_length_5825_cov_5.478683, whole genome shotgun sequence.
ACCESSION   DAOTMX010000082 DAOTMX010000000
VERSION     DAOTMX010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5825
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11675_length_5825_cov_5.478683"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="P5573_03385"
     CDS             <1..353
                     /locus_tag="P5573_03385"
                     /inference="COORDINATES: protein motif:HMM:NF013753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRT27140.1"
                     /translation="ELKHTRYLWLKNPERLSAQQRSILETLCLKWYFWATHSRLEPIE
                     QWAKTLKKHRQGILNGFQSLVNNGILEGINSMIQAARARARGYRTVKNSITMIYLISG
                     ERDFRLPTCNSEEP"
     gene            complement(731..1390)
                     /locus_tag="P5573_03390"
     CDS             complement(731..1390)
                     /locus_tag="P5573_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27141.1"
                     /translation="MSKARTLAALAVLICVFLTGNIVSAADHPHGKTAKKMVWTQSAV
                     DLNKTMRKLWAQHMEWTYAAVAAFATNSPIYDATAARLMKNQEDIGNSIRSFYGDAAA
                     EGLTRLLKDHIAGAIQVCKDAKANDKSALEKSIAAAYANAQEIADFLAKANRHWPKEI
                     VRDMLRGHIDTTLVYATALLAGKYAEGIAEYSKAEDHMMVLADALTEGLVAAFPRKFR
                     K"
     gene            complement(2077..2760)
                     /locus_tag="P5573_03395"
     CDS             complement(2077..2760)
                     /locus_tag="P5573_03395"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27142.1"
                     /translation="MDFRDDRAQREGIFPQFGELTEQSYRLAISKLIERDRGLAGVVA
                     KWGIPPFRTHPPGFQGIVLAILAQQVSLESARATFRKLENAISAVTLDAFLTLHTDTL
                     REIGFSRQKASYVRKIAEDLVAARIDLEALQWIDDTEAGTRLMQIRGVGKWTADTYLL
                     FSLRRSDVWPSGDLALEKAVSEIKGLPSKPRTEEVDRIAAAWKPLRAVAARILWHQYL
                     NQRGRFHSA"
     gene            3370..4038
                     /locus_tag="P5573_03400"
     CDS             3370..4038
                     /locus_tag="P5573_03400"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27143.1"
                     /translation="MQPAEKARTPINAPLGQALIKRGILTQAQLDKALEIQKQRKNLY
                     LGEILQNLGVNQNKINETLDFINKRKKIGDILIDLGLITPAGLERALKDQKLIQSKMG
                     TRKPLGILLFEMGLISYRDYMTALSKHFSIRIVTLEGNRVNPSLQDMLGRKYVCKHDV
                     LILEDDGRTVKIALGAPTPFLMQEIRKSIPSHREVIFYLAHPMEIAATYRMMYDPFSE
                     NRDR"
     gene            complement(4182..5087)
                     /locus_tag="P5573_03405"
     CDS             complement(4182..5087)
                     /locus_tag="P5573_03405"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HRT27144.1"
                     /translation="MPLGKLRIFFLATRPQFLLLAVTLGVLGACIAWYDGFFNVWGAA
                     LAGIGLVLAHASCNALNDYFDYKSGVDKVATVTPFSGGSGLIKRGLLRPEEVARLGTV
                     CLLLALLIGLYFCVTKGWLLLPLIVIATACVVLYSPVILKRRWPEWSPGLGLGILPVL
                     GMYFSQTGTYTLHALIASVPSGFLVHNLLLLNEFPDIEADKVGGRKTLPITMGRRGAA
                     AVYTAFSTLTYVWIVGAVIFKAMPVFTLLALFTLPLAIKAMGGAFRNEDMGILMPAMA
                     RNVVVVLLTQLFTAVGYVLSGLFRF"
BASE COUNT         1439 a         1575 c         1483 g         1328 t
ORIGIN      
        1 ccgaactcaa gcacacccgg tatctttggc tcaaaaaccc cgagcgtctg tcggctcaac
       61 aacgctcgat cctggagacg ctttgcctca aatggtactt ctgggcgacc cacagccgcc
      121 tggaacccat cgagcagtgg gcgaaaaccc ttaaaaaaca ccggcaagga atcctcaacg
      181 ggttccagtc tctcgtcaac aacggcatcc tcgaaggcat caacagcatg atccaggctg
      241 ccagagccag agcccggggt taccgaaccg ttaagaactc catcacgatg atctatctca
      301 tcagcggcga acgggacttc agattaccca cttgtaatag cgaggaacca tgacttctgc
      361 ctcatccgca cataagaaaa aaattcctgt tttctacaac cacgaattct caaggtatgg
      421 cgactgatca tccagaagtc ggctggaaaa aaaatcctgc gaaagaacag gagctgccag
      481 tataaagagt tcttgaccta cctccccctt gagctgtggt gaggtttgga aacatgctcg
      541 catcgccatt tctattaccg ctaagagaaa gtggctaaca ggggcagaaa atttaaaaaa
      601 tagaccccat tgccacaggc agaagcccct cagcaataga gttatttgat tccggacccg
      661 aaaactaatc agggggcatg cgggcgcacc cctgtaaccc atttatgtca tcagagaaac
      721 aagacgcgtc ttattttctg aattttcgag ggaacgctgc cacaaggcct tccgtcaacg
      781 catctgcaag aaccatcatg tggtcctctg ccttgctgta ttcagcaatg ccttcggcat
      841 atttcccggc caacaatgcc gtcgcgtaca ccagggtcgt atcgatgtga cccctcaaca
      901 tgtcgcgaac gatttctttc ggccagtgcc tgtttgcctt tgccagaaaa tcggcaattt
      961 cctgagcatt tgcatacgct gcagcgatcg atttttccag tgcggacttg tcatttgcct
     1021 tcgcatcttt gcaaacctga atcgctccgg caatgtgatc ctttaagagc ctggtaagcc
     1081 cttccgcagc tgcgtcgccg tagaaagaac ggatggagtt gccgatatct tcctgatttt
     1141 tcatgagccg ggcagccgtc gcgtcgtaga tcggggagtt tgttgcaaac gccgccaccg
     1201 cagcgtacgt ccactccatg tgctgagccc atagtttgcg catcgtttta ttcaagtcta
     1261 ccgcactctg ggtccatacc attttttttg cagtcttacc gtgaggatga tccgctgcag
     1321 acacgatgtt gcctgtcaga aaaacgcaga tcaatacggc tagggcggca agtgttcttg
     1381 ctttgctcat gttcgtcctc tcctttcttt cttcgatggt gacatgaata tgcgaacgcg
     1441 cgcgcggcat acatcgcgtt gccggcagat tcagtgtttc gtcgtttccc gctcgtcggt
     1501 tcccggggag ttgtcgtcga aacctgccgc ccggtcgacg agccgtgtgc atcgggatca
     1561 gatctttttt tgccttacag aatgaccggc cccgccctcg agggtgcgcc ggatcaatga
     1621 aggggttatc catttggaga caatcccgtt tttttgttgt acctcaaagg cggctgatga
     1681 actctccacc gtgcgatcac tttcgaaggt ccttcctccc gtggtgcagg tgacacatcc
     1741 atacggaggt atccacaaga accattttat tccacggccg ttctccggcg aggtattctt
     1801 tttaagctcc tttccgttcc tcccgatttt ctaagctcct tactgcgctc cacagcaatt
     1861 aacgcttcta atccaagctt ggcgagtgta gtttttttaa cacctgtcag ctttgaaact
     1921 ttcccaatga ggcctctccg atgttcgaga tcatcctcat attatcctcc tggaaatcta
     1981 tatgcatgat tcgtgcgtat tttgtcaata agctcttgat caagataacg acgaagctca
     2041 gtggcatgac gcccgccacg ctcactgcag gggatgtcag gctgaatgga atcgaccccg
     2101 ttgattgagg tattgatgcc ataatatcct cgctgcgacc gccctcaatg gtttccaagc
     2161 cgctgcgatt cgatcgactt cttccgtcct tggttttgac ggtagcccct tgatctcgga
     2221 aacagccttc tctagcgcga gatcgcccga aggccagacg tctgatcttc tcaacgaaaa
     2281 caacaaatac gtgtcagcag tccatttccc gacccctcga atttgcatga gccttgttcc
     2341 ggcttctgtg tcgtcgatcc actgaagggc ttcgagatcg atcctggccg caacaagatc
     2401 ttcggcgatc tttcgaacat aagaggcttt ctggcggcta aagcctattt ctcgtaaagt
     2461 gtctgtatga agagtaagga atgcatccag ggtaactgca gagatcgcat tctccagctt
     2521 acggaaagtc gcccgggcgg attcaagaga tacttgctga gcgagaatcg cgaggacaat
     2581 accctgaaag cccggtggat gagtccggaa aggaggaatc ccccacttgg cgacgacccc
     2641 tgcaagacct cgatctcgtt cgatcagttt ggagatcgca agacgataac tctgctccgt
     2701 cagttcaccg aactgaggaa atatcccctc tcgttgtgct ctatcgtccc gaaaatccat
     2761 aaccatcctt catttcagcc tgacgtgaaa gatcggcggc cgacgcgctt tttcgcacga
     2821 gtctgctgga gcgatctgtt cgtgggttat tcattttccg gttcctcttt caaagcttgt
     2881 ggaagttcag ttaccgattc gggaaacgct tctttggttc atcgtatgtc cgtaaggact
     2941 ttttagatgc gggggagatg cgagggtcag gtcttgcttt ttgcctttac gcagaataac
     3001 cggccccgtc atcgaggacg tgtcagaatc atccaggggg ttgcggcgac aaccgcaacc
     3061 ctcttttcaa acggccggac gcagccaggt atgcttggta tcaccctgct tcggagagcc
     3121 agattatgag gccaggggca gaaacaccag gagaataaca actgtgtctc ctttcaagga
     3181 taatttttag gtttcgcgcc ggcaggtatt gttctttagc atggcattga gggtgatcag
     3241 gatctttctc atacaggcca cgatggcccc ggccgtccca attcttttcc caatgcctgt
     3301 catttacggc ttaaatatat tctcataaaa accgatatta ttaccaagaa ccaccggagt
     3361 gctttcatca tgcagccagc ggaaaaagcc agaaccccga tcaatgcgcc cctgggccag
     3421 gccctgatca agagggggat actgactcaa gcccagctcg acaaggctct ggaaatacaa
     3481 aagcagagaa aaaatctata cttgggagag atattgcaga atctcggcgt taaccagaac
     3541 aagatcaacg agactctgga ttttatcaat aagaggaaga aaatcggcga catattgatc
     3601 gacctcgggt tgatcacccc cgcggggttg gaacgggccc tcaaggacca gaaactgatc
     3661 caatccaaaa tgggtaccag aaaacccctg ggtattctgt tgttcgagat ggggctgatc
     3721 agctacaggg attatatgac ggccctctcc aagcactttt ctattcggat cgtcacactc
     3781 gaaggcaaca gggtaaaccc atcgctgcag gacatgctgg gcaggaaata tgtctgcaaa
     3841 cacgatgtgc tgatcctgga agacgatggg cgcaccgtaa agatcgcgct gggagcacca
     3901 acgcccttcc ttatgcagga gatcagaaaa tcgatcccat cccatcggga agtcatattc
     3961 tatttagctc accccatgga aatagcggcc acttacagga tgatgtatga cccgttttcc
     4021 gagaaccgcg accgatgaac accgctgaac ggtcctgacg gcagccctct ataggacacc
     4081 atgatcatca aggggttgaa gcctttcgct tcaactcctt ttttatgctc tgtccgggag
     4141 ggaggccgag tccgtcaggg ggattttttt attcgttctg ctcagaacct gaacaggccg
     4201 gagagaacat aaccgacggc cgtaaacaac tgggtcagaa ggacgacgac aacgttacgg
     4261 gccatggccg gcatcagaat acccatgtct tcgttccgaa atgcaccccc catcgctttg
     4321 atggcgagcg gcagcgtaaa cagcgccagc aatgtgaata cgggcatcgc cttgaaaatg
     4381 accgcaccca cgatccacac atatgtgagg gtggaaaagg cagtataaac ggcagccgca
     4441 ccccggcggc ccatggtgat gggcaaggtc tttctgcccc ccactttatc cgcttcgata
     4501 tcgggaaact cgttcagaag aagcaggtta tggacaagaa aaccggacgg gaccgaggca
     4561 atgagggcat gcagggtata ggtgccggtc tgggaaaaat acatgccgag gaccggaagg
     4621 atccccagac cgagtcccgg tgaccactcc ggccagcggc gtttgaggat tacagggctg
     4681 tacaggacga cgcaggccgt ggcgatgaca atcagcggca atagaagcca gcccttcgtc
     4741 acgcagaagt agaggccgat caacaaggcc agcaggaggc acaccgtccc gagccgggcg
     4801 acctcctccg gcctcaggag cccccgtttg atgagtccgc tgccgccgct gaaaggcgtg
     4861 acggtcgcga ccttgtccac cccgctttta tagtcgaagt aatcgttcag ggcgttgcag
     4921 ctggcgtgcg ccaggacaag cccgatgccg gcgagagcag ccccccagac attaaaaaag
     4981 ccgtcgtacc aggcaatgca ggcaccgaga acaccgagcg tcacggcaag aagcagaaat
     5041 tgcggtctcg tggccagaaa gaatattctc aatttcccga gcggcatacg ggagggaaac
     5101 tacgcttttc caaggattat gtcaaatggg gactgaagct cggaatagag gcgttttctg
     5161 gcatttgagg acagacgatg aacgtccaag ggttacggaa aaagccgagg ccttttgtat
     5221 tccggctgag cggggcggat gggtctgact cgcccgcgtt tacgaaccct ttcatactct
     5281 ctcattagcc cacgcccgga gaaaatatgt aataaatgag acggttgtcg tatctatttc
     5341 ccgggagatc agccgtaaaa tgaatcattc ctgaacgcga aaccagcgaa gatccaggca
     5401 tggatagggg ccggggttgc acgatctttt gccggaaaaa cgagaggcgg atcgacagga
     5461 gcaacgccgg cgacggtgtc acgaccgttc atgacatcaa aaaacagatc gtcgaggccg
     5521 taagagcgat gctcagtcat cacggaggga gaggctcacc atcaaagaca aggatgggaa
     5581 ggtcaagatc aaagacacga tcaggacctc gacggggtct ccgggtccgg acactctcct
     5641 cttgtcggcg cgacgaaaat catatcgttt gcccgatcct gcatctcacc tgaaaaaata
     5701 acccgttaac ctcgtgaaag gaaaggggtt cagaccgaga acggttgatc cctttcccct
     5761 tttataccgc cctgttgcct cataaataga tgttaccgaa ggatggtcag gtagagcgat
     5821 cgttg
//
LOCUS       DAOTMX010000083         5767 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_11813_length_5767_cov_3.608718, whole genome shotgun sequence.
ACCESSION   DAOTMX010000083 DAOTMX010000000
VERSION     DAOTMX010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5767
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11813_length_5767_cov_3.608718"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="P5573_03410"
     CDS             <1..250
                     /locus_tag="P5573_03410"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HRT27145.1"
                     /translation="PKAADYFRRRKIFPTQEIRETKPDGSLIVSLKVGNYGEVWDILK
                     SWLPNIRIIGPETLLNGFSAEIKKWLSWQTSSNVNMQA"
     gene            296..2320
                     /locus_tag="P5573_03415"
     CDS             296..2320
                     /locus_tag="P5573_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27146.1"
                     /translation="MNTIIKLISYPVKWLWDLFRALVDFIWSVVRAILRSCLFIVVGF
                     LYLIPAFIFSFDGSRNKFVFLFYLIILILPISIKGLLTKHYLVKLFSGIFIMASGFMV
                     YQYYLSFLNIYLVTWLVIASGLLILRELLMDDDSNRILALSTSVFSAILILALSYNVF
                     FSGDFFLKDQNPTCARLVSINDLPGISASSRELIRKTDVLIVPERVCEVQQNEYAFAA
                     RPVKNKFFSQDVYYLKSKPEDFDELVAEINYVSNFQESYIKSYAFHGGQGFVRAALDT
                     FKFLFNILLHPIDTISSFFDFIVSIPDIVLNCFRDPSSIVASTLGALNSHMDETEQEI
                     ARQNNLKTEKIMLPETVAVIKHQRNIEFGGAVGFQAVDLYAGYRGASLVKYLPYFKNT
                     EKAVRLNKFIKHAPDSGTNKLVRETGKEAEAQIAKGEVKQITDLQDVQNSAISRERIT
                     LNQNSLEKHPVMNKVDDGQPRSQTHPQERNTAPGQKDIKTPKGKEEFYPKAQEDLKSA
                     GINTIPEEGLKKVGSHWKSRPDCVGYRENQLIVSEIKSGMEPPTSSSWRKAQKSDTTQ
                     FTAVRKQVKALEDAGEVSKEVGGWMIIAKGQVEDYARKMGRTWDVPNPSMKKGKKLYG
                     SLSVPPDQKENVLTAFAELKKRKKLDIDLNKIEIIESEGSVSFIWPLGTI"
     gene            complement(2335..4131)
                     /locus_tag="P5573_03420"
     CDS             complement(2335..4131)
                     /locus_tag="P5573_03420"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRT27147.1"
                     /translation="MRYVVYKDFRDSLSWLHERGGRYQKIAGKVFQVIGKSNIFEGKD
                     PFDGVPVTNHGEHRIKHCVKRDLGDWCRLVTIVNNGVTALCFAGTHDDCDKWLDRNRG
                     LELALNDKGEITEILTSTDIKVDDKRINTNQGLSSQRLVKKISARHIEKIAGGVSWSI
                     MQQFAELKSTATDEEILDLSCKIEDTEKQNVFFDVFSKLREDDVEGAIKSIELYVDSL
                     KRLEEVPKDKVNTLPLSDNFIDFEDLEPVLFDHYVKTANFKKWMLFMHPEQKKLVERN
                     FSGPAKLVGVSGSGKTAIVVKRAVHLAKQYLGKRILVLTLNRALASLIEDLIDEVCPD
                     EGCRGRIDVYSFWKFCQLKLQSFAPQNRRLYDELTWKTKEHVDEIWGEYFECKVNNDD
                     AKVLTPVNRSLLSRSVFPKDYIRQEFDWIRSACPADAREKYYDIERHGRTEPFIRDYR
                     AMILKGLKAWEDMMAFVGVTDYLGLSTALHRHIERIKPEYRCVLVDEVQDFGTIELDI
                     VRRLVPEKENNLFLCGDIAQQVCFKHHKLSDAGINVTGRSFSIKKNYRNSREILAAAF
                     GFTSMSTSRLRSSVNQMLALWERVRVGSRILT"
     gene            complement(4162..>5767)
                     /locus_tag="P5573_03425"
     CDS             complement(4162..>5767)
                     /locus_tag="P5573_03425"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRT27148.1"
                     /translation="EVGIAAFDVEENTPDADFGRKIDRVRRYRDEIIDLYCPRIQIRS
                     GLEPVTCGLIFPNTETKVLMQTAAKNRFVHPLYLPVSGKQEFERGEIAKIFPGAGRKG
                     SPAISDELASDLRSWLVEPDFQTLRRVPLELDKDQRRLVTTRTETGYRRIKGPAGSGK
                     SVVLAARAAQLASEGKSVLVITYNITLMNYLRGLALRWDTPAPVINKGVTWLNFHHWC
                     KRVMQESDSESEYNKMWQAHFDKDGDETSKNEVLKREMPALASKIIDGDVAGYIQRYD
                     AIIVDEGQDFHPLWWQTLRKVLNPGGEMLLAADMTQDVYDTGQQWTDQVMAGAGFRAP
                     WVRLRYSYRMPPNLVAKANQFAKRRLKRDNVDYPVSDQQPELNLYPCKMRWVQTSREK
                     LVDCCTAEILKMPIHADPDILAIPDVVCICDLRKTGLQVARRIAAMKIGVAHTFGEND
                     RRSKMSFMMDKAPVKITTLQSFKGWETRALVLCVERVRGYRSLAAIYAGITRVKRHDK
                     GSYLTVVCSDPQLREDGRDWPEYAES"
BASE COUNT         1349 a         1598 c         1390 g         1430 t
ORIGIN      
        1 cccgaaagcg gccgactact tcaggcgccg caagatcttc cccacgcagg agatcaggga
       61 aacaaaaccc gacggctcat tgattgtaag cttgaaggtg ggaaactatg gggaagtctg
      121 ggatattctg aaatcctggc tgccgaatat aagaatcatc gggccggaaa cgttactgaa
      181 tggcttcagt gctgaaataa aaaaatggtt gtcgtggcaa acatcttcaa atgtcaacat
      241 gcaagcctga ccgcatcttc acattgactt ttgtccagca aaagcaaggc tgtgaatgaa
      301 tacaataatt aaattgatat cctaccccgt caaatggctc tgggacttgt ttcgtgccct
      361 ggtggatttt atctggtctg tggtcagggc catcttgcgt tcatgtctct ttattgtcgt
      421 gggttttctg tacctcatac ccgcctttat tttttccttt gacggcagcc gcaacaaatt
      481 tgtttttctg ttttatctga tcattttaat cctgcctatc agcatcaagg gacttctgac
      541 caaacattat ctcgtcaagc tcttttccgg cattttcatc atggccagtg ggttcatggt
      601 ttatcagtat tatctatcct ttctgaatat ttatctcgtt acgtggcttg tgatagccag
      661 cgggcttctg attctcagag aacttctgat ggatgatgat tcaaacagaa tccttgcctt
      721 atcgacctcc gtcttctctg cgatattgat cctagccttg tcatataatg tatttttctc
      781 tggtgacttt ttcctgaaag atcaaaaccc gacttgtgca cgacttgtca gtataaacga
      841 cctgcccggt atatccgcat catcgcgaga actcatcagg aaaactgacg tactgatcgt
      901 acccgagcgg gtttgcgagg ttcagcaaaa cgaatacgca tttgcggcga ggcctgtaaa
      961 aaacaagttt ttttctcaag atgtttatta cctgaaatcg aaacccgagg atttcgatga
     1021 gcttgttgcc gaaattaatt acgtctcaaa ttttcaggaa agctacatca agagctatgc
     1081 ctttcacggg ggccaaggat tcgtcagggc tgctttagac accttcaagt tcttattcaa
     1141 tatcctgctg catccgattg ataccatttc atcgtttttt gattttatcg tctctatccc
     1201 ggatatcgta ctgaattgtt ttcgcgatcc ttcctccata gttgcatcaa ctctcggtgc
     1261 actgaacagt cacatggatg aaactgaaca ggaaattgcg cggcaaaata acctcaaaac
     1321 cgagaaaatc atgttgccag aaacggttgc agtcataaaa catcagcgga atatcgagtt
     1381 cggcggtgcg gttggcttcc aagccgttga tctttatgct ggctacaggg gtgcatcgct
     1441 tgtcaaatat ctgccatatt tcaagaatac cgaaaaagca gtgaggctta acaagttcat
     1501 aaagcacgct cccgatagcg gcacgaataa actggtacgt gagaccggca aagaggctga
     1561 agcccaaatt gcgaaaggtg aggtcaagca gataaccgac ctacaggacg tgcagaactc
     1621 ggcaatcagt cgtgaaagaa ttacattgaa tcagaatagc ctagaaaagc accctgtcat
     1681 gaataaagtc gacgatggcc agccccgaag ccagacacat ccccaggaaa gaaatacagc
     1741 gcccggacaa aaagatatca aaacaccaaa aggcaaagaa gaattctacc caaaggccca
     1801 agaagacctt aagagtgcag ggataaacac aatacctgag gagggcctga agaaagttgg
     1861 atctcactgg aagagtcggc cggactgcgt agggtacaga gagaatcaac tcatcgtcag
     1921 tgaaataaaa tcaggaatgg agcccccaac ctcgagttcc tggagaaaag cacaaaaaag
     1981 cgacacgacc caatttacgg ccgtgaggaa acaagttaag gcccttgaag acgcgggcga
     2041 agtgtccaaa gaagtaggcg gttggatgat catcgcaaaa ggtcaggttg aggattatgc
     2101 acgaaaaatg gggcgtacat gggatgttcc aaacccatcc atgaagaaag gaaagaaatt
     2161 atatggcagc ttgagcgtcc cgcctgatca aaaggaaaat gtcctgacgg cattcgcaga
     2221 attgaaaaaa cgaaagaagc tcgatatcga cctaaataaa attgaaatta tcgaaagcga
     2281 aggttccgtt agcttcatct ggccactggg cacgatataa atgtagcgag ccattcatgt
     2341 taaaatgcga gacccaaccc tcactctttc ccagagcgcc aacatctggt ttaccgagga
     2401 gcgcaacctg gaggtactca tcgaggtgaa cccgaaggcg gccgctagga tctccctgct
     2461 gttcctgtag ttcttcttga tcgagaagga acggccggtc acgttgatcc ctgcgtctga
     2521 cagcttgtgg tgtttgaaac agacctgctg cgcaatgtcg ccgcacagga atagattgtt
     2581 ttccttctca gggactagtc ttcgcacgat gtccagctca atagtcccga agtcctgaac
     2641 ctcgtcgacg agtacgcatc ggtactcggg tttgatcctt tctatgtgcc tgtgcagcgc
     2701 cgtcgacaac cccaggtagt ctgtgacccc gacgaacgcc atcatgtctt cccaagcctt
     2761 gagccccttg aggatcatcg ccctgtaatc cctgatgaac ggctccgtcc gcccatgtct
     2821 ctcaatatcg tagtactttt cccttgcatc cgccggacag gcgctccgga tccagtcgaa
     2881 ctcctgcctg atgtagtctt tagggaacac cgagcgcgac agcagcgacc gattgactgg
     2941 tgtcaacacc tttgcgtcgt cgttgttgac cttacactcg aagtactccc cccagatctc
     3001 gtcgacgtgc tctttcgtct tccacgttaa ctcgtcatac aaccggcgat tctgaggcgc
     3061 aaagctctgc agcttcaact ggcagaattt ccagaaggag tagacgtcga tcctccccct
     3121 gcacccctca tcggggcaga cctcgtctat caggtcctcg atcaagctcg ccagggctct
     3181 gttcagggtt aaaacaagga tcctctttcc taggtactgc ttggctaggt ggacggcgcg
     3241 cttgaccaca atggctgttt tccccgaacc gcttaccccg accagcttcg cggggccgct
     3301 gaagttcctc tcgacgagct tcttctgttc cgggtgcatg aagagcatcc acttcttgaa
     3361 gttcgccgtc ttgacatagt gatcaaacag cacaggttcg aggtcttcga aatcgatgaa
     3421 gttgtcgctc agcgggagag tgttcacctt gtcctttgga acctcctcta gccgcttcag
     3481 gctgtcaaca tacagctcga tgctcttgat ggccccttcg acgtcatcct cccgtaattt
     3541 gctgaagacg tcgaagaaaa cattctgctt ttcagtgtct tcaattttgc acgacagatc
     3601 caagatctcc tcgtccgtgg cagttgactt caactctgcg aactgctgca tgatcgacca
     3661 ggaaacaccg ccagctattt tttctatgtg ccgtgcagat atttttttga ccagccgctg
     3721 gctggacaac ccttggttcg tgtttattcg cttgtcatcg accttgatgt ccgtcgaggt
     3781 caggatttct gtgatctcac ccttgtcgtt cagcgcaagt tcaagccccc gatttcggtc
     3841 aagccacttg tcacagtcat cgtgcgttcc ggcaaagcac agtgccgtca ccccattgtt
     3901 gacgatggtc acgagacgac accaatcacc caaatctcgc ttcacacagt gcttgatccg
     3961 gtgttcacca tgatttgtca ccggcacgcc atcgaagggg tctttcccct cgaatatgtt
     4021 actcttccct ataacctgaa agacctttcc ggcgattttc tgataacgcc cgcccctttc
     4081 gtgcagccac gacaacgagt ccctgaagtc cttgtaaacg acgtacctca cgtgcgaacc
     4141 ccctccgtcc aattttgagt ttcagctttc tgcgtattca ggccagtccc ggccatcttc
     4201 ccgcaactgg ggatctgagc aaaccacggt caagtagctt cccttatcat gccgtttgac
     4261 ccgcgtgatt cccgcgtaaa tggcggcaag cgaccggtac ccccgcacac gttccacgca
     4321 aagcaccaag gccctcgtct cccatccttt gaagctttga agcgttgtga ttttcacagg
     4381 ggctttgtcc atcatgaatg acatttttga gcgccggtca ttttccccaa acgtgtgggc
     4441 caccccgatt ttcatggcgg caatgcgcct cgccacctgc agcccggtct ttctcaaatc
     4501 acagatgcag accacatccg gtatggccag gatgtcaggg tcggcgtgga tgggcatttt
     4561 caatatctct gccgtgcagc agtcgacaag cttttcccgg cttgtctgca cccacctcat
     4621 cttgcacggg tacagattca gctccggctg ctggtcagaa accgggtaat cgacgttgtc
     4681 cctcttcagg cgccgtttcg cgaactgatt ggccttggcc accaaattcg gcggcattcg
     4741 gtagctgtac cgcagccgga cccacggggc ccggaaaccc gcgccggcca tgacctggtc
     4801 cgtccactgc tgccccgtgt cgtaaacgtc ctgggtcatg tcagcggcga gcaacatctc
     4861 accgccaggg ttgagcactt tgcgcagcgt ctgccaccag agggggtgaa agtcctggcc
     4921 ttcatccacg atgatcgcgt catatcgctg gatgtaaccg gccacatccc cgtcgatgat
     4981 ttttgaagcg agcgctggca tttccctttt caaaacctca tttttgctag tctcgtcgcc
     5041 gtctttgtcg aaatgtgcct gccacatctt gttgtattcg ctttccgagt cgctctcctg
     5101 catcacccgc ttgcaccagt ggtggaagtt gagccacgtg acccccttgt tgatgaccgg
     5161 cgcaggagta tcccagcgca gggccagccc ccggaggtag ttcatcaggg ttatgttgta
     5221 tgtgatcacc aggacagact tgccttcgga ggccagctgc gccgcccgtg ccgccagcac
     5281 cacggacttc ccggagcccg caggcccttt gatccgccgg taaccggtct ccgtcctcgt
     5341 ggtcacgagc cggcgctggt ccttgtccag ctccagcggc acgcgccgca gggtttggaa
     5401 atccggctcg acaagccagc tccgcaagtc gctggccagc tcgtcggaga ttgcaggaga
     5461 gccttttcgg ccggcgcccg ggaagatctt cgcgatttcg cctctttcga actcttgctt
     5521 gcccgaaacc ggcaaatata gagggtgcac aaaccggttc ttggctgccg tttgcatcag
     5581 cacctttgtc tctgtgttgg ggaatatgag accgcacgtc accggctcga ggccggaacg
     5641 gatctgaatt cgggggcagt ataggtcaat gatctcgtcc cgatacctgc ggacccggtc
     5701 gatcttcctc ccgaagtcgg catcaggcgt attctcctcg acgtcgaagg ccgctatgcc
     5761 gacctcc
//
LOCUS       DAOTMX010000084         5747 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_11854_length_5747_cov_3.393008, whole genome shotgun sequence.
ACCESSION   DAOTMX010000084 DAOTMX010000000
VERSION     DAOTMX010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5747
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11854_length_5747_cov_3.393008"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /gene="gmd"
                     /locus_tag="P5573_03430"
     CDS             <1..856
                     /gene="gmd"
                     /locus_tag="P5573_03430"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRT27149.1"
                     /translation="NLHYGDLSVSGQMTDLLACIRPDEIYNLGAQSHVRVSFDMPEYT
                     GDVTGLGTLRLLEAIRKTGIKTKFYQASSSEMFGAAPPPQNELTPFQPQSPYAAAKVY
                     AHHMVQNYRKAYNIFACNGILFNHESPRRGETFVTRKVTRAATRIKLGLKDKLYLGNL
                     DARRDWGYAGDYVEAMWLMVQQDEPDDYVVATGETYSVREFVEKVFEKLDLDYRKHVI
                     IDKRYFRPTEVDVLLGDAAKARKKLGWSPKVGFDRLIDMMVKADMELAEKEKTLRDAG
                     YEWVEKRM"
     gene            861..1823
                     /locus_tag="P5573_03435"
     CDS             861..1823
                     /locus_tag="P5573_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HRT27150.1"
                     /translation="MVDLRQKRITVTGGRGFLGRYVVRELEKRGCRRIGTFSSRDYNL
                     VLAEDVKRMYDDLKPDIVIHLAARVGGIGYNRENPAVLFYDNLMMGAQLIHEGYTRKI
                     EKFVAVGTICAYPKFTPVPFREEDLWNGYPEETNAPYGLAKKMMLVQSQSYRSQYRFN
                     SIFLLPVNLYGPGDNFDPRSSHVIPALIKKCVDAVSEGKEEIVVWGTGRATREFLYVE
                     DAAEAIVLAAERYDGSEPVNCGAGFEISIRNLVDLIVELSGYRGRVLWDESQPDGQPR
                     RMLDTSRALAAFGFKAQTDFRTGLKETIEWYRRQVSVKRHPAEG"
     gene            1957..2244
                     /locus_tag="P5573_03440"
     CDS             1957..2244
                     /locus_tag="P5573_03440"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HRT27151.1"
                     /translation="MDKDTKNFMIQLTYASSVGFAMVLAVFGGLLLGSWLDRKLGTGH
                     KLTLLFFLVGIIAGLRNVYTMVKRNFPNEGEEIQRRIKSEPHRKRPSPKKA"
     gene            2246..2605
                     /locus_tag="P5573_03445"
     CDS             2246..2605
                     /locus_tag="P5573_03445"
                     /inference="COORDINATES: protein motif:HMM:NF015834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HRT27152.1"
                     /translation="MRNWIGLGVLLTLSLVFMPLNFTLGVLLGGLISIINFYWLYLSL
                     RKVFQNLTNKAKTALMVRYYIRFAVTGVVLYFVITGTPADVIGLLVGLSVVVMNIIVS
                     AVVEASKYNILKTREVK"
     gene            2611..3264
                     /gene="atpB"
                     /locus_tag="P5573_03450"
     CDS             2611..3264
                     /gene="atpB"
                     /locus_tag="P5573_03450"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HRT27153.1"
                     /translation="MHSFLYLEWIPFPAHVTYTWLTMILLALLSVAATRRMDIYPNKI
                     QNIMEVVVGAIENLLIETMGPHGKAYFPLIATLAIFILTSNLIGLIPGFESPTSNLNT
                     TVSMALCVFFMTHIVGIKIHGFKYIKQFLGPVWWLTPLMLPIEIISHLTRPLSLSVRL
                     FGNIQGGHIVLAVLFILVPFLVPLPILALKVFIAIIQTLVFALLSMMYIAGAMEGAH"
     gene            3696..>4419
                     /locus_tag="P5573_03455"
     CDS             3696..>4419
                     /locus_tag="P5573_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27154.1"
                     /translation="MTTPIQQKLRDKLITAKKWAEMVKSGDWINRGGPGSDTTVTMEA
                     LAARFGDGPGDLKNIELWNQAITLGYNFLDAADHEAKYHVVHESFLLGSLRKLAGNGY
                     KGLDWKHWGWAIGMDAEYARFYRKNRAERAMDWGVQACAVPEAGFVNASYGVNNFMIT
                     AKSCKKFVCEIRSDYAWCEGGRETMYLPIDDVDYFVEVDVDDPKYQWPYIAEKEIQPK
                     PEEKAIAQNILSIMRDGDCIQVG"
     assembly_gap    4420..4429
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4430..5214
                     /locus_tag="P5573_03460"
     CDS             <4430..5214
                     /locus_tag="P5573_03460"
                     /inference="COORDINATES: protein motif:HMM:NF024732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HRT27155.1"
                     /translation="ALPTAVVIALGESNLRHIGTHTEMIGEYAFTLTDAGVLDNSRKT
                     IDKGRCAWAYIMPVDTPRYYERVHRNPYFAGYDIGYTNNLAVISQLDNFIAVNNFAQM
                     DVRGQDAAGNVAGRPISSTGGQFQFIVGASMARGGRGVLAATSRDARGRSRFVPMLDP
                     GSVVAVPDQLVTYICTEYGIVNIQGCTDAEKATKIISVAHPDDREWLAKETVDKLGIK
                     LNHWMFTSAPDRRFPTPAQMKDHKYGHLDLELKPKPIAKRDD"
     gene            complement(5304..>5747)
                     /locus_tag="P5573_03465"
     CDS             complement(5304..>5747)
                     /locus_tag="P5573_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRT27156.1"
                     /translation="EMPARIEEVHHAEEEGVQFNFLTNPVEYIGDEDGWVKKMVCNRM
                     ELGEPDGSGRRRPIVIPGSEFTIDVDTVVVAIGTTANPVIPRTTPGLEVNKWGYIVTK
                     DGTGETTRKGVYAGGDIVTGSATVILAMGAGRQAAKAVDEYIRTL"
BASE COUNT         1325 a         1616 c         1584 g         1212 t
ORIGIN      
        1 gaacctccat tacggagacc tctccgtctc cgggcagatg acggacctgc tcgcctgcat
       61 caggcccgac gaaatctaca acctgggcgc ccagagccat gtccgcgtca gcttcgacat
      121 gcccgaatac acgggagacg tcaccggtct ggggacgttg aggcttctgg aagccatccg
      181 gaagacgggc atcaagacga agttctatca ggcctcttcg agtgagatgt tcggcgccgc
      241 tccgccgccg cagaacgaac tcaccccatt ccagccccag agcccttacg cagcggccaa
      301 ggtctatgcc catcacatgg tccagaacta ccggaaggct tacaacatct ttgcctgcaa
      361 cggaatcctt ttcaaccatg aatctccccg gaggggagaa acttttgtga ccagaaaggt
      421 gacccgggct gctacgcgca tcaaactggg gctaaaggac aagctctacc tcgggaacct
      481 cgacgccagg cgggattggg gttacgccgg agattacgtc gaagccatgt ggttgatggt
      541 gcaacaggac gagccggatg attacgtcgt cgccaccggt gaaacttatt cggtgagaga
      601 gtttgtcgag aaagtcttcg agaaactcga cctggattac cggaagcatg tcattataga
      661 caagagatat ttccggccga cggaagtcga cgtcctcctc ggtgacgccg ccaaggcaag
      721 gaaaaaactc ggatggagtc ctaaagtcgg tttcgaccgg cttatcgaca tgatggtcaa
      781 ggccgatatg gagctcgcgg aaaaggaaaa aacgctgcgg gacgccggtt acgaatgggt
      841 ggagaaaagg atgtgacggg atggtcgacc tgagacaaaa gcgcataaca gtcaccggcg
      901 gaagggggtt tctcggccgt tatgtggtaa gagaattgga gaagcgcggg tgtcggcgca
      961 taggcacctt cagctctcgc gactacaatc tcgtcttggc cgaggatgtg aagaggatgt
     1021 acgacgacct aaaaccggac atagtcatcc atctcgccgc aagggtcggt ggcatcggct
     1081 acaacaggga gaacccagcg gtactcttct atgacaacct catgatgggc gcccagttga
     1141 tacacgaggg ttacacccgg aaaatcgaaa aattcgtcgc cgtcgggacg atctgtgcct
     1201 atccgaagtt cacgcccgtt cccttccggg aggaagacct ctggaacggt tatcccgaag
     1261 aaacaaacgc cccttacggg ctggcgaaga agatgatgct ggtccagtcg cagtcatacc
     1321 gcagccagta taggttcaac tccatcttcc tcctgcccgt caacctctac ggaccgggtg
     1381 acaacttcga cccccggtcg tcgcacgtca tccccgcctt gatcaagaag tgcgtcgacg
     1441 ccgtctcgga aggcaaagaa gaaatagtgg tctggggaac ggggcgggcg acgagggaat
     1501 tcctttacgt ggaagacgct gccgaggcga tcgtccttgc cgcggaaaga tacgacggca
     1561 gtgaacccgt caattgcggc gccggctttg agatttccat acgtaatctc gtcgacctga
     1621 tcgtcgaatt atcgggttac cgcggacgtg tcttgtggga tgaatcgcag ccggacggcc
     1681 aaccccggag gatgctggac acgtcgaggg cactggcggc cttcggcttc aaggcgcaaa
     1741 cggatttcag gaccggcctg aaagagacga tcgaatggta cagaaggcag gtctcggtca
     1801 aaaggcaccc ggcagaagga tgatcgtcca cgttttaagc cgtcttacgg gtctgctgta
     1861 gcttccggct ccgcgttttt ggccttatac ccccataacc cgttgtccgt tttatcgttg
     1921 actttatcgg tcacgtatgt tacaaggcgc ccgctcatgg acaaagacac aaagaacttc
     1981 atgatccagc tgacctacgc cagtagcgtg ggttttgcga tggttcttgc agtcttcgga
     2041 ggtcttctgc tcggttcttg gctggaccgg aagctgggca cgggacataa gctgaccctc
     2101 ttgtttttcc tcgtcgggat catcgcgggg ctcaggaatg tctataccat ggtcaagcgc
     2161 aacttcccca atgaggggga ggagattcaa aggcggataa agagtgaacc ccaccgtaaa
     2221 agaccctctc caaaaaaagc ttgaagtgag gaactggata ggactcgggg tgttgcttac
     2281 gctgagtctc gtttttatgc ccttgaattt cactctcggg gtcctgttgg gcgggttgat
     2341 cagcatcata aacttttact ggctctattt aagcttaaga aaggtcttcc aaaacctgac
     2401 taacaaggcc aagacagccc tcatggtccg atattacatc cgcttcgctg tgacgggggt
     2461 tgtactgtat tttgtcatca ccggtacacc ggccgatgtg atcggccttc tcgtaggcct
     2521 gtcagtcgtc gtcatgaaca tcatcgtgtc ggctgtcgtc gaagcgtcga agtataacat
     2581 actaaaaacc agggaggtta agtaacaaag atgcattctt tcctgtacct tgagtggatc
     2641 ccctttccgg cccacgtgac gtacacgtgg ttgactatga tattattggc tctcctttct
     2701 gtcgccgcaa cccgccggat ggacatttac cccaacaaaa tacagaacat catggaagtt
     2761 gtcgtgggcg ccatagaaaa cctgctaatc gagactatgg gtccccatgg aaaggcttat
     2821 ttccccttga tcgcaacgct cgccatcttc atcttgacat cgaacctcat cggtctcata
     2881 ccagggtttg agtcgcccac atcgaacctc aacacgacgg tgtccatggc cctttgcgtg
     2941 ttcttcatga cccacatcgt gggcatcaaa atccacggct tcaaatacat caagcagttc
     3001 ctggggccgg tctggtggct caccccgttg atgctgccta tcgaaatcat aagccacctt
     3061 accaggccgc tatcgctttc ggtgcgacta ttcggaaaca tccagggcgg gcacatagtc
     3121 ctcgccgtgc tcttcatcct ggttcctttc ctggtgccgt tgccgatcct ggccctgaag
     3181 gtcttcatcg caatcatcca gacattggtc ttcgctctcc tctcgatgat gtacatcgcc
     3241 ggcgcgatgg agggggctca ttagtggaga cgggcttgag agaagcaccg gtctggccga
     3301 atgacagtac ctctgccgtc tttagagtca tcacggggtc aaaatcccca agctgtcgtg
     3361 ctgggccttg actaatcccg gctcggtact cgagccgggc atacccacaa catcttgaat
     3421 tcatggcgtt acaccactga ttttcagcgg agtagcgcgt caggtgtgta tgcccagtgt
     3481 ggcaccacgg ggaactgaaa ggtcccttcc tccggtcgga acagacttga caacgggatg
     3541 agcgaggacc tcaagataat gtcacttaga aaggggggat caaggcaatc aactgaaaat
     3601 ttttctgcgg taaataaatc tgacgaagcc ttaaccaaaa tttcaatgga gggagtatct
     3661 acatgcctca aaagaagatc gaaaacaaag taacgatgac gacaccgata cagcaaaaac
     3721 ttcgtgataa actgatcact gccaaaaagt gggcagagat ggtcaagtcc ggtgactgga
     3781 taaaccgcgg cggtcccggt tccgacacta ctgtcaccat ggaagccctc gctgcgagat
     3841 tcggtgacgg cccgggcgac ctgaaaaaca tcgagctctg gaaccaggcg atcacgctcg
     3901 gttacaattt cctcgacgcc gccgaccatg aggcgaagta ccatgtcgtc catgagtcat
     3961 tcctcttggg atcgctgcgc aagctggcag gcaacggcta caagggtctc gactggaaac
     4021 actggggatg ggcgatcggc atggacgccg aatacgcgcg gttctaccgc aagaacaggg
     4081 cggagcgtgc catggactgg ggcgtccagg cctgcgccgt tcctgaggca ggattcgtta
     4141 atgccagcta cggcgtcaac aactttatga tcaccgcaaa atcctgcaag aagttcgtct
     4201 gcgagattcg ttccgactat gcctggtgcg agggcggacg ggagaccatg tatctgccca
     4261 tcgacgacgt cgattacttc gtcgaggtgg acgtggacga cccaaaatac caatggccct
     4321 acatcgcaga gaaggaaatc cagcccaagc ccgaggaaaa ggcgatcgca cagaatattc
     4381 tctcgataat gcgggacgga gactgcatcc aggtcggcan nnnnnnnnng cgcccttccc
     4441 accgcggtgg tcatcgccct tggggaatca aacctgcgtc acatcggtac tcacaccgag
     4501 atgatcggtg aatatgcctt tacccttacg gatgcaggtg ttctcgataa ttcgcgcaag
     4561 accatcgaca agggacggtg cgcatgggct tacatcatgc ccgtggacac accccgttac
     4621 tacgaacgag tgcaccgcaa cccctacttc gccggctacg acatcggtta cacaaacaac
     4681 ctcgcggtca tatcccagct cgacaacttt atcgccgtga acaacttcgc ccagatggac
     4741 gtccgcggcc aggacgcggc gggtaatgtg gccggccgtc ccatctcctc cacgggcggt
     4801 caatttcagt ttatcgtagg cgcctctatg gccaggggag gcaggggtgt ccttgcggcg
     4861 acctcacggg atgcgagagg caggtcccgc ttcgtcccga tgctggatcc gggcagtgta
     4921 gttgccgttc ccgaccagct cgtcacttac atctgcaccg agtacggtat cgtaaacatc
     4981 cagggctgta cggacgccga gaaggcgacg aagatcatat ccgtcgccca cccggacgac
     5041 agggagtggc tggcgaagga gacggtggac aaactgggca ttaagctcaa ccactggatg
     5101 ttcacgagtg ccccagaccg ccggttcccc acgccggctc agatgaaaga ccacaaatac
     5161 ggtcacttgg acctggaact caaaccaaag cccatcgcca agcgcgacga ctgatcaggg
     5221 aagagggttt gtatatacaa gaaaggcagg gcggatgccc tgcctttcca cttttgacga
     5281 cccccgatcg gggtgctacg ccctcacaat gtccttatgt attcgtcgac ggccttcgcc
     5341 gcctgacgac cggcgcccat ggcgaggatg acggtagcgg agccggtgac gatgtctccg
     5401 cccgcgtata cgcccttgcg ggtcgtttct cccgtgccgt ccttcgtcac aatatagccc
     5461 catttattta cttcaagccc cggcgtagtc ctggggatca ccgggttggc cgtggtgccg
     5521 attgcgacga cgacggtgtc tacatcgatc gtgaattccg aacccggaat cacgatgggc
     5581 ctgcgcctgc ccgatccatc gggctcgccg agttccatcc ggttgcagac catttttttc
     5641 acccagccgt cttcgtcgcc gatgtactcg acggggttgg taagaaaatt aaactgcacg
     5701 ccttcctcct cggcatggtg aacctcctct atcctggcgg gcatctc
//
LOCUS       DAOTMX010000085         5733 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_11889_length_5733_cov_3.934836, whole genome shotgun sequence.
ACCESSION   DAOTMX010000085 DAOTMX010000000
VERSION     DAOTMX010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5733
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11889_length_5733_cov_3.934836"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            41..595
                     /locus_tag="P5573_03470"
     CDS             41..595
                     /locus_tag="P5573_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27157.1"
                     /translation="MKKLLVLICLGIVVIVAGQAMAQETTEQWRPIQLSLTPDIAIFD
                     RSTRIKGFTLGICSENPQDAFALGIVNGSSRSSAGLSIGVANYADSYAGVQISAFNYA
                     KENFTGWGVGVANVAKGAMKGLQTGAVNYAGNLSGLQLGAVNYAEKASGGVQIGAINI
                     IRENTWSDFPNGLGPGMIIFNWRF"
     gene            complement(694..1092)
                     /gene="gcvH"
                     /locus_tag="P5573_03475"
     CDS             complement(694..1092)
                     /gene="gcvH"
                     /locus_tag="P5573_03475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HRT27158.1"
                     /translation="MAKENPADRRYSREHEWAKDNGDGTITTGITDYAQEMLTDIVYV
                     ELPALGKKVRQGETIAVVESVKSVSDVYSPVSGEVIDVNGVLENKPELINQDAFGEGW
                     IAKVRMDDPGELKNLLDAAAYTSFLQEIGH"
     gene            complement(1157..2254)
                     /gene="gcvT"
                     /locus_tag="P5573_03480"
     CDS             complement(1157..2254)
                     /gene="gcvT"
                     /locus_tag="P5573_03480"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HRT27159.1"
                     /translation="MQRTPLYDRHLKLGARMIDFGGWEMPVQYTTVIDEHRATREAAG
                     LFDTCHMGEIDVTGPGSYAFLQRVLSRDLAGMTDGRMRLSLLTDHRGGIIDDLTVYRL
                     GDDRYRLVTNAVTKDKDLIWLRRVVQQEGFSGVEIMDVTDRTGKLDLQGPRSAEILQS
                     LVADSLSGLKFYDFIETEILGLGAVVSRSGYTGEDGFEVYVPAEAVGKVWDKILEAGT
                     PLGLKPAGLGARDTLRLEAGMMLYGNEMNESVTPFEVVYGWATDLKKDFIGSEALRKQ
                     KGQGIRRKLTGFELTGRGIARHGYAVFKNGRAIGEVTSGTFSPTLQKAIGMAFVPVEH
                     SEPGTQVDIRIRDNRAEARVVPLPFYKRKIV"
     gene            2772..3047
                     /locus_tag="P5573_03485"
     CDS             2772..3047
                     /locus_tag="P5573_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27160.1"
                     /translation="MKKLASTLLVAGFVVLSTATFVFAEYSAAGAAEFPFFHLGALIV
                     GGLIIVSLKHKYQKLYLSEAVGSFALYTVLIALFTSPVVELIKGLVG"
     gene            3092..3334
                     /locus_tag="P5573_03490"
     CDS             3092..3334
                     /locus_tag="P5573_03490"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HRT27161.1"
                     /translation="MVTAKRTGQGQTTMQGLSGILMLSAWGFAIVICSFAFMYAGYWI
                     DRALNTAPSFMLGLFLLALFLCVGRLYQEAWLKRKR"
     gene            complement(3394..3972)
                     /gene="def"
                     /locus_tag="P5573_03495"
     CDS             complement(3394..3972)
                     /gene="def"
                     /locus_tag="P5573_03495"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRT27162.1"
                     /translation="MAETKTWALWKDGKVNPEEAKVLRRRASEVPVPYNDAARKDIKA
                     VVDSFLSRDDALGLAAPQVGISKRIIVFKTKNFNERERIKSREEYEVLVNPRLVQVRG
                     AEEKMPEGCLSCPDVSVEVTRATEIKVRALDEEGRKVNKRYTGFLARIVQHELEHLDG
                     KLIIDHGGTVSFPKKKEAFFTSLFGGEPGKQE"
     gene            4098..5354
                     /locus_tag="P5573_03500"
     CDS             4098..5354
                     /locus_tag="P5573_03500"
                     /inference="COORDINATES: protein motif:HMM:NF006385.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase family protein"
                     /protein_id="HRT27163.1"
                     /translation="MDTLINRVITRVARRQINRSMTFSPQESPALPPADKKGKRLLYL
                     HVPFCERLCPYCSFNRVPFEEKLCRAYFSALRKEILLYRDKGYDFNGIYVGGGTPTVL
                     VDELESTLSLARECFSVGEISVETNPDHLTEGRLVPLERAGVKRLSVGIQSFDDALLK
                     AMDRYDKYGSGRRIAERMKSVMGRFDTLNADMMFNFPSQTGASIENDLDTLAEIAVDQ
                     VTYYPLMVSDSTRRQVEKVLGSVDEGREYGYYRLISRRILEEYRFSSAWCFSRKTAEA
                     VKIVDEYVVDYDDYAGLGSGSIGYLDGVCYANTFNIEDYIHCVGMGRIPLMASRTFGI
                     KERVHYDFLMKLFGGSLDLIALGKKYGVSLYRYLWLPIFFFRLVGGIERRGNRLFLTE
                     RGRYQWVVMMREFFTAVNNFRDFCRS"
     gene            5375..>5733
                     /locus_tag="P5573_03505"
     CDS             5375..>5733
                     /locus_tag="P5573_03505"
                     /inference="COORDINATES: protein motif:HMM:NF023807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HRT27164.1"
                     /translation="MDERRSKLLRGLPKIDEVLLLLKKGDTFAKAPKDLVVDACREIV
                     AAIRENILTERADTPPPSAEEVAERVRRRVESMQSAKLRRVINATGVILHTNLGRAPL
                     CKEAINRINEVAGGYSN"
BASE COUNT         1177 a         1607 c         1557 g         1392 t
ORIGIN      
        1 tacacttaat catcataatc tcagtgaaag gagataggcg atgaaaaagt tgctcgttct
       61 tatctgtctc ggaatcgtcg tgattgttgc cggccaggca atggctcaag aaactaccga
      121 acaatggcgg ccgatacaac tcagtctgac tcccgatatt gccattttcg acaggagcac
      181 gaggattaaa ggattcactc tcggcatatg tagcgaaaac ccccaggatg cctttgccct
      241 gggaatcgtc aacgggtctt cgaggtcgag cgccgggttg agcatcggtg tcgccaacta
      301 tgcggatagt tatgccggcg tgcagatttc ggcgttcaat tacgcgaagg aaaacttcac
      361 cgggtggggc gtcggcgtcg ccaacgtcgc gaagggggcc atgaaaggtc ttcagaccgg
      421 cgccgtgaat tacgcaggca acctctcggg cctccagttg ggtgccgtca actatgccga
      481 gaaggcatcc gggggcgttc agatcggggc gataaatatc attcgcgaga acacgtggtc
      541 cgacttcccc aacgggttgg ggcccggaat gatcatcttc aactggcgtt tctgaaagaa
      601 ggggcgtgcc agacaccctc ttcatccgta tacgacaata gcctcgtccc ggcgacaggg
      661 aaatccggca caatacgggt tttgctgtcg ccgtcaatgt cctatctctt gcaagaacga
      721 cgtgtatgcc gcggcgtcga ggaggttttt cagttcgccc gggtcgtcca tcctgacctt
      781 cgcgatccag ccctcgccga aggcgtcctg attgatcagc tctggtttgt tctcgagaac
      841 cccgttaacg tctatcacct cgccgctcac cggtgaatag acgtcggata cggacttcac
      901 cgattctacg accgctatgg tctcaccctg cctcaccttc ttcccgaggg cgggaagctc
      961 gacgtagact atgtcggtaa gcatctcctg ggcgtaatcg gtgattcccg tcgttatggt
     1021 accgtctccg ttatccttcg cccactcgtg ttcccgcgaa tacctccggt cagccggatt
     1081 ctcctttgcc atcccattcc tcccctcttc cgtcgcagct gaggctcatg catgaactct
     1141 caatggttga ccatatttaa acgattttcc tcttatagaa aggcaatggg actacacggg
     1201 cttcggcccg gttgtcccgg attcggatgt cgacctgcgt gcccggttcc gagtgctcga
     1261 cgggaacgaa ggccatgccg atggccttct ggagtgtggg cgagaacgtc cccgacgtca
     1321 cttcaccgat cgcccggccg ttcttaaaga cggcgtaacc gtgacgagcg atgccccgtc
     1381 cggtcagctc gaacccggtc agttttctcc ggattccctg tcctttctgt tttctgaggg
     1441 cctcgctccc gatgaagtcc tttttgaggt ccgtcgccca gccgtagacc acttcgaagg
     1501 gcgtgaccga ttcgttcatc tcgtttccgt aaagcatcat gccggcctcg aggcgtaagg
     1561 tgtcccgggc gccgagccct gccggcttca ggccgagcgg tgtcccggct tcgagtatct
     1621 tatcccagac cttcccgacc gcttccgcgg gtacgtatac ctcgaaaccg tcttctccgg
     1681 tgtagccgct ccgggatacg acggctccta aaccgaggat ttccgtttcg atgaaatcat
     1741 agaacttgag ccccgagagg gaatcagcga ccaatgactg aaggatttcc gcggagcggg
     1801 gaccctgcag gtccaatttc ccggttcggt cggtgacgtc catgatttcg acgccggaaa
     1861 atccttcctg ctgaaccacg cgccgcaacc agatgaggtc cttgtctttc gttacggcgt
     1921 ttgtcaccag ccggtatctg tcatcgccga gcctgtacac ggtaaggtcg tcgatgatcc
     1981 cgccccggtg gtcggtcagg aggcttagcc gcatccggcc gtcggtcatt cccgccaggt
     2041 cgcggctcag tactctctgc aggaacgcgt acgaacccgg tcctgtgacg tctatctcac
     2101 ccatgtgaca ggtgtcgaaa agtcccgccg cctcgcgtgt cgccctgtgt tcgtcgatca
     2161 cagtcgtgta ctggaccggc atctcccagc cgccgaaatc gatcatcctg gcaccaagtt
     2221 tcaggtgccg gtcatacagg ggggttctct gcatctctat atcctacggt tgggtcgacg
     2281 ccgcccgccc gatgcaaata caattctaag cgggtcattc gatacgcttc gagacctttg
     2341 aatttttgat taaatcacct tactgtcatt ccgtgcttga cacggaatcc agtgttttta
     2401 atctgttctg gattccgact tccgtcggaa tgacatgaag gggcgttttt caaaggtctc
     2461 gcttggcctg ttttcacgac gaatgccacg ttgagtacta cattcggccg ttgacggcaa
     2521 ggttaaaggg tctttttcga ccggcaccgt cgtttgaatg attcattcat cccgttgaac
     2581 gtttcattca gcggagaggg ggttttctcc ccggggagcg ggaccgaaga tgccgtctat
     2641 ttgtcaaata tgatgcaata tcaatatgat atacaatact ttcctgggcg taggggagct
     2701 ggcacgataa tttcatacat atagagtaaa aaaaccggaa tgattctaac atccaaagga
     2761 ggaaacagaa aatgaaaaag ttagcatcca cattgttagt tgcgggtttt gtggtcctgt
     2821 ctacggcgac tttcgtgttt gccgagtaca gcgcagcagg agcggccgaa ttccccttct
     2881 tccacctcgg cgccttgatc gtgggcgggc tgatcatcgt gtctctcaag cataagtacc
     2941 agaaacttta cctgagcgag gccgtcggca gctttgccct ctacaccgtc cttatcgcgc
     3001 tcttcacctc gccggtagtg gagctcatca aagggttggt cggctgatac gtttagaacg
     3061 aacggcatca cagcaagtaa aagggctcgc catggtgacg gcaaaaagaa caggacaggg
     3121 acagacgacg atgcaagggc tatcggggat tctgatgctg agcgcctggg gtttcgcgat
     3181 cgtcatctgt tcctttgcct tcatgtacgc gggctactgg atagacaggg ccctgaatac
     3241 ggcgccgtcg ttcatgctcg gcctctttct gcttgccctt tttctctgtg tcgggcgcct
     3301 ttaccaggag gcctggctga agcgaaagag atagccccaa aaggcctgac tgtaaaaaac
     3361 ccttttcccc cgaaaggggt tttttgtttt ggtttattcc tgtttgcccg gctcgccccc
     3421 gaaaaggctt gtgaaaaagg cctctttctt cttcgggaat gaaaccgtcc cgccgtggtc
     3481 gatgatcagc ttcccgtcga ggtgttccag ttcatgctgg acgatgcggg caagaaatcc
     3541 cgtgtaacgc ttgtttacct tgcggccttc ttcgtcgagg gcacggacct tgatctccgt
     3601 cgctctcgtt acctcgacgc tcacgtcggg gcatgagagg catccctcgg gcatcttttc
     3661 ctccgcaccg cggacctgga ccaacctggg gttgacaagg acttcgtatt cttctctgct
     3721 tttaatccgt tccctctcat tgaaattttt ggtcttgaag acgatgatcc tcttgctgat
     3781 gccgacctgg ggcgcggcga gaccgagggc gtcgtctctc gacaaaaacg aatcgacgac
     3841 cgccttgatg tccttccgtg ccgcatcgtt atacgggacg ggtacctccg aggcacgcct
     3901 cctcaggact ttcgcctcct ctgggttgac ctttccgtcc ttccagagtg cccatgtttt
     3961 cgtttctgcc atacctcagt ccttctgctc cgacatgaac cgagcacctc tataacaccg
     4021 gcacgtcttt ttttcaacac cgcccagttc ttgctaattc tccggtactt tgttataaat
     4081 ccggccggga ggaaatcatc gataccttga tcaaccgagt gataacccgg gttgcacggc
     4141 gccaaatcaa caggtcaatg accttttccc ctcaggaaag cccagccctt ccgccggctg
     4201 ataaaaaagg aaaaaggctg ctttacctcc atgtcccttt ttgcgagcgg ctctgtcctt
     4261 actgttcctt caatcgggta cccttcgaag aaaagctctg cagggcctat ttctcggccc
     4321 ttagaaaaga gatcttgctt tatagagata agggctacga ttttaacggc atctatgtgg
     4381 gcggagggac gcccactgtt ctcgtcgatg agctggaatc gaccctgtct cttgcccggg
     4441 aatgcttttc cgtcggggaa atatcggtcg agacaaaccc cgaccatctc accgaaggaa
     4501 gacttgtccc gctggaaagg gcgggtgtaa agcggctttc cgtgggtatc cagagcttcg
     4561 atgacgccct gttgaaggcg atggacaggt acgataagta cggaagcggt cgtcggattg
     4621 cggagcggat gaagtccgtg atgggacgtt tcgacacgtt gaacgccgac atgatgttca
     4681 attttccgtc ccagaccggg gcgagcatag agaacgacct cgacacgctt gccgaaatcg
     4741 ccgtcgacca ggttacctat tacccgctca tggtctccga ctcgacccgg agacaggtag
     4801 aaaaagtgct ggggagtgtc gacgaggggc gggaatacgg gtactaccgg ctgatttccc
     4861 gacggattct cgaggagtac cgcttttcct ccgcctggtg tttttcccgc aaaacggctg
     4921 aggccgtgaa gatcgtggat gaatacgtgg tggattacga cgactatgcg ggcctcggta
     4981 gcggttccat aggttacctg gatggcgtct gctatgccaa taccttcaac atcgaggatt
     5041 acatccactg cgtgggaatg ggcaggatcc ccttgatggc ctcgcggacc ttcggcatca
     5101 aagagcgggt ccactacgat tttctgatga aactcttcgg cggaagcctc gacctgattg
     5161 ccctgggaaa gaaatacggt gtgagccttt atcggtatct gtggcttcct atattctttt
     5221 ttcgtttggt cggcggtatc gaacgccggg gcaaccggct tttcctcacg gaacgcgggc
     5281 gttatcaatg ggtggtgatg atgcgggaat tcttcactgc tgtcaacaac ttccgtgatt
     5341 tttgcaggtc ctgactttta agacagaggg tgagatggac gagagacgat cgaagctgct
     5401 gcggggcctt cccaagatcg acgaagtttt gctccttcta aagaaagggg acaccttcgc
     5461 aaaagcaccg aaggacctgg ttgtcgatgc ctgccgtgag attgtcgcgg ccatcaggga
     5521 aaatatcctg acggaaaggg ccgacacccc ccctccctcg gccgaagaag tggctgaacg
     5581 ggtccgcagg cgggtcgaat cgatgcagtc ggcgaaattg cgccgggtca taaacgccac
     5641 cggcgtgatt ctccatacga acctaggtag ggcgcctctt tgcaaagagg ccatcaaccg
     5701 gataaacgag gtcgccgggg gttactctaa ctt
//
LOCUS       DAOTMX010000086         5722 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_11907_length_5722_cov_4.571378, whole genome shotgun sequence.
ACCESSION   DAOTMX010000086 DAOTMX010000000
VERSION     DAOTMX010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5722
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11907_length_5722_cov_4.571378"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="P5573_03510"
     CDS             complement(<1..434)
                     /locus_tag="P5573_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HRT27165.1"
                     /translation="MKIEAPHSESENLKVVKAAGVVGLATGLSRVFGYIRDMVVAHFF
                     GAGMATDAFFVAFRIPNLLRRLLAEGSLTVSFVPVFMEYLKRKSREDALELANVSFTL
                     LSIILVVISVAGVLLSPLIVTIMAPGFMKTPDKYALTVFLTRL"
     gene            complement(504..782)
                     /locus_tag="P5573_03515"
     CDS             complement(504..782)
                     /locus_tag="P5573_03515"
                     /inference="COORDINATES: protein motif:HMM:NF014633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HRT27166.1"
                     /translation="MKETERGLIFYVRVVPRSSRCEAAGIQDDALRIKITSPPVEGKA
                     NDECVRFLADLLGVKKSQVEIISGHKGRKKTVAVTGLKKADIDAFFRT"
     gene            complement(821..1123)
                     /locus_tag="P5573_03520"
     CDS             complement(821..1123)
                     /locus_tag="P5573_03520"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HRT27167.1"
                     /translation="MFVFGNLLKALAFVIDAALTIYIWVIVARALVSWVNPDPYNRIV
                     IFLYRVTEPALRPVRRLMPLRNMGIDISPVIVLLIIYFLKIFLVDTLSQTAYHFTP"
     gene            1349..2170
                     /gene="proC"
                     /locus_tag="P5573_03525"
     CDS             1349..2170
                     /gene="proC"
                     /locus_tag="P5573_03525"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HRT27168.1"
                     /translation="MLKGKKISIIGGGKMGEVLAKGMSTNDQVSAADVTVTDVIKERL
                     EYLQGKYGVSVTDDNLRAAKDADIVILAVKPQSMGEVLGPLKEVVDETKLVISIAAGI
                     PIGFIEGCLEKGPRVIRVMPNTPALVGEGAAALAPGKSATEADLNVARGIFDAVGMSV
                     VVKEESIDAVTGLSGSGPAYVFIMIEALSDGGVLMGLPRDVSLRLAAQTVLGSAKLYL
                     ETAGKHPGDLKDMVTSPGGTTIAGIKALEDGGFRAALINAVEAATRRSQELGKGK"
     gene            complement(2213..3025)
                     /locus_tag="P5573_03530"
     CDS             complement(2213..3025)
                     /locus_tag="P5573_03530"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HRT27169.1"
                     /translation="MGSIREAAVDIGGKKYVIASDDDYLKGMGSRFEPEMVKLFRTVA
                     NGSEVTLDIGANIGCTAILFAQLSKKVYAFEPSPTTFALLERNIRRSGLRNVFLQNIG
                     LGAEPGEFTLTFSPANRSGAFVSNQTQASAGHTVEKITIRRTDEAVISLGLPRVDFMK
                     IDVEGFEGHVLRGATQTLSAHRPAVVLELNHWCLNAFQRTSIPDFLDLLRSMFPILLA
                     VDGSNYLDLHDTSESYIVMYHHILHMRFPNILAAFDESQLGGFRSSYRHRFD"
     gene            3499..4491
                     /locus_tag="P5573_03535"
     CDS             3499..4491
                     /locus_tag="P5573_03535"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HRT27170.1"
                     /translation="MPNKLIALQEAVSRYTRDGMVYSSGGGLPVGSESVVFGKEMVRQ
                     GRKGIHYLTHCCSQQVNLFCAAGVCDRVEAAFSGLEVYGFANGVRRAVESGRVLWEDW
                     SNLSMTLRFLGGALRWPFVPATVNIGSDNQWRSAFKPDEYPCKSKIPDVTDPFTGKVY
                     GALPVARPELAVIHVTMSDVEGNAIFLGTEWSRFELARAADKVILQADFIVDTDCMRQ
                     YPNLVRIPDLLVEAVIPWQFGAWPQCSVGVYDSDEDHMRAMNKMLGTDEGTREFRAKY
                     IDSWHTHEEYLALIGKVAIEKIMNNATTHLMDPYRKWVKSDEEIKKLMKESKFS"
     gene            4506..5288
                     /locus_tag="P5573_03540"
     CDS             4506..5288
                     /locus_tag="P5573_03540"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HRT27171.1"
                     /translation="MEFTRQEMMAVATGREIRDGDLCIFGVGLSMLAGYFALAYHAPN
                     AKAMTEGGVYGSTPVGGLPWGIEDNRISANATCFTGALDALGFLVASGRCDLGIIGAA
                     QVDKFGNVNTTGFWEDPTSPHFGPPKTRLTGSGGANDIACGCKRVVIMLAHEKKRFAE
                     KVDYITSPGYLDGGNAREKYHFVGGGPVAIVTTLGILRPDPVTKEMLIDSYFPFSSVE
                     EMKANTAWDLKVSPGVKVTPEPTAEELKVLRAVDVTNVLKRT"
     gene            5330..>5722
                     /locus_tag="P5573_03545"
     CDS             5330..>5722
                     /locus_tag="P5573_03545"
                     /inference="COORDINATES: protein motif:HMM:NF023222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HRT27172.1"
                     /translation="MALKTGDQYVESIKKMRPNVYKWGELIRDVTTHPATRLHIQSVK
                     RSYDLSNDPAKASIYTAKSHLTGEVVHRWNSLMQSAEDVMGNSNMKREQYHQSGTCQG
                     ATCAGWTAMNVLWDATWQMDKDLGTDYHE"
BASE COUNT         1319 a         1513 c         1722 g         1168 t
ORIGIN      
        1 agccgcgtca ggaagaccgt cagggcatac ttatcggggg tcttcatgaa acccggcgcc
       61 atgatggtca cgatcagggg cgagaggaga acacccgcga cggagatcac gacgaggatg
      121 atggagagca gggtgaagga gacgttcgcc agttccaggg cgtcttccct cgactttctc
      181 ttcaggtatt ccataaagac ggggacgaag gagaccgtga gcgaaccttc ggccaaaagg
      241 cgccggagga gattgggaat ccggaaggcc acgaaaaagg cgtccgtcgc catcccggcg
      301 ccgaagaagt gggcgaccac catgtcgcgg atgtaaccga agacgcggct gagtccggtg
      361 gccaggccca ccacgccggc ggccttcaca accttgaggt tttccgattc gctgtggggt
      421 gcttctattt tcatcttttc aagcagcagg tgaacggtgc aaggtgatcg gtgagctacg
      481 atccgtcgca cactaccttc aggtcatgtc cggaaaaacg catcaatgtc cgccttcttc
      541 agcccggtga cggccaccgt cttttttcgt cccttgtgtc cggagatgat ttcgacctgc
      601 gattttttta ccccgagcag gtcggcaagg aagcggacgc actcatcgtt cgcctttcct
      661 tcgacgggcg gagaggtgat ttttattctc agggcgtcgt cctggatgcc cgccgcctcg
      721 cagcgggacg acctcggtac tacccgaaca tagaaaatca agcccctctc ggtttctttc
      781 aacggtagca tcggcgaaat tccctgtcgg gggtcctttt ttatggagtg aaatggtagg
      841 ccgtttggga tagggtatcc accagaaaga ttttcaggaa gtagatgatc aggaggacga
      901 tgaccggcga gatgtcgatg cccatattcc tcaagggcat caatctcctc acgggcctga
      961 gggccggttc ggtaaccctg tacaagaaaa tgacgatccg gttgtaaggg tctgggttca
     1021 cccaggagac cagggcgcgg gcaacgatga cccatatata gatggtcagc gctgcatcga
     1081 ttacaaaagc taacgccttg agcaggttcc cgaaaacgaa catcagtctc tacctctggg
     1141 ttcatcaaat tttcgtgaaa ataatgtttg agtatcgaaa tgtcaaggtc aatgttttcc
     1201 cgaggttttt gttgacagcc cttgaaaatt gcgcaatcgt ggcaggaaat ttccgagtta
     1261 gcttggttct cccgctacgg gagggcacac gaaaggggca tggttaggaa ctttttttga
     1321 cattttggat gaaggaggag aacaggccat gttgaagggg aaaaagataa gtatcatcgg
     1381 cggcggaaag atgggtgagg tcctggcgaa ggggatgtcg acgaacgatc aggtctccgc
     1441 ggcggacgtg acggtgacag atgtcatcaa ggagcgcctc gagtacctgc aggggaagta
     1501 cggcgtatcg gtaacggacg acaacctgag ggcggcgaag gatgccgaca tcgttattct
     1561 tgccgtgaag ccccagtcga tgggggaggt cctggggccg ctgaaagagg tggtcgacga
     1621 gacgaaactc gtgatctcga tcgcggcggg cattccgatc ggcttcatcg aaggatgctt
     1681 agagaaaggc cccagggtca tccgtgttat gcccaacacc ccggcactgg tcggtgaagg
     1741 ggctgccgca ctcgcgcccg gtaagagcgc gaccgaagca gacctcaacg tggcgcgggg
     1801 tatattcgat gccgtgggga tgtccgttgt cgtcaaggaa gaatcgatcg atgccgtgac
     1861 gggactgagc ggcagcgggc cggcctacgt cttcatcatg atcgaggccc tgtccgacgg
     1921 cggggttctg atgggacttc ccagggatgt ttctctcagg ctggcggcgc agacggtgct
     1981 cggctcggcc aagctctacc tcgaaacggc cggtaagcat cccggtgact tgaaggatat
     2041 ggtcacctcg ccgggcggga cgacgatcgc gggcatcaag gccctcgagg acggaggctt
     2101 cagggcggcg ctgatcaacg ccgtcgaggc cgcgacccgg cggtcacagg agttggggaa
     2161 gggtaaatag cgcctggaga agtaggtcgt catcctctac ccggctaacc gatcagtcga
     2221 atcgatgccg ataggaagac ctgaatcccc cgagctgact ttcgtcgaaa gctgcaagga
     2281 tgttcggaaa tctcatatgc agtatgtgat ggtacatcac gatgtagctc tcgctcgtgt
     2341 cgtgcaggtc caggtagttt gagccgtcca ccgcaaggag aatagggaac atcgaccgca
     2401 ggaggtcaag aaagtcgggg atagatgtcc gttggaaggc gttcaagcac cagtgattca
     2461 gctctaatac aacggccggc ctgtgagcgg aaagcgtctg cgtcgcgccc cgtagcacat
     2521 gcccctcgaa tccttctacg tctattttca tgaaatccac ccgcggtaga cccaaagata
     2581 tgaccgcttc gtcggtccgg cggatggtta ttttctccac tgtatgaccg gcgctggcct
     2641 gtgtttgatt tgagacaaaa gcccccgacc tgttcgcggg ggagaaggtt aacgtgaact
     2701 cgcccggttc cgcgcccaac ccgatatttt ggaggaacac gtttcgcagc cccgacctcc
     2761 ggatattcct ctcgagcaac gcgaaagtgg tcggagacgg ctcgaaggcg tagacctttt
     2821 ttgatagctg cgcgaacagt atggcggtac acccgatgtt ggctccgata tccagcgtga
     2881 cctcgctgcc gttcgccacc gttctgaaaa gcttcaccat ttctggctcg aaacggctgc
     2941 ccataccctt caagtagtca tcatcggagg caatgacata cttctttcct cctatgtcta
     3001 ccgccgcttc ccttatagat cccatgttga tcctcgttat tatgtaatcg atcgatagcc
     3061 agctaagagg ttccttgccg ctacatgaac cttcccgaca gcacgcacat tgacacaagt
     3121 atcggccgaa ggcaagttaa gagaaatatc gcttgcatta tcatttacac atgctataag
     3181 tgcgcgcatg aagtatgact gcggaggaag ctgatccgca gataacacgg cctaccgacc
     3241 tgtaaaacct gtataggtca tcgatttgtg tttttcttat tttaaacgag aaacccatgg
     3301 aagtagtctt caagtttttt tcttttccga tacccacgca agtaaaacat atccagcata
     3361 tcctgcagaa ggcgattttc ctgattcaaa gcggccacag ttccatagaa tcggcaaaag
     3421 tctgacgtat ttagattttt ctgcgttaat caacaaaaaa caacagagca gcctttcggg
     3481 caaaaaggag ggattgaaat gcctaacaaa ctgattgcgc ttcaggaggc cgtttcgagg
     3541 tacacgcggg acggcatggt ctatagcagc ggtggaggct tgccggtggg gtccgaatct
     3601 gtcgtcttcg ggaaagagat ggttcgccag ggacgcaagg gaattcacta tctgacgcac
     3661 tgctgcagtc agcaggtcaa cctgttctgc gccgctgggg tctgcgacag ggtggaagcc
     3721 gccttttccg ggctggaggt gtacggcttt gcgaacggcg tccggcgggc cgtagaatcc
     3781 ggcagggtgc tatgggagga ctggtccaac ctctccatga ctctccggtt cctgggaggg
     3841 gctctccgct ggccatttgt cccggctacc gtgaatattg ggagcgacaa ccagtggcgg
     3901 agtgccttca agcccgacga gtacccctgc aaatccaaga ttcccgatgt cacggatccc
     3961 ttcaccggga aggtgtacgg cgccctgccc gtggcaaggc ccgaactggc tgtcatccac
     4021 gtgacgatgt ccgacgtgga gggaaacgcg atcttcctcg ggacggagtg gagccggttc
     4081 gagctcgccc gggcagcgga taaggtcatc ctgcaggccg acttcatcgt ggacaccgac
     4141 tgcatgcggc aataccccaa cctggtgcgg atccccgacc tcctggtcga ggcggtcatt
     4201 ccctggcagt tcggcgcctg gccccaatgc tctgtcgggg tgtacgacag cgacgaagac
     4261 cacatgaggg cgatgaacaa gatgctgggg acggacgagg gaacgaggga attccgggcg
     4321 aaatacatcg acagttggca tacccatgaa gagtaccttg cactgatcgg gaaggtcgcg
     4381 atcgagaaaa tcatgaacaa cgcgacgacc catctgatgg acccttacag aaagtgggtc
     4441 aagagcgacg aagaaatcaa gaagctgatg aaagaatcga aattttcctg aagggaggga
     4501 atcacatgga atttacgaga caggaaatga tggccgttgc gacgggaagg gagatacgtg
     4561 acggcgacct ctgtattttc ggcgtggggc tcagcatgct ggccggttac ttcgccctcg
     4621 cgtaccatgc gcccaatgcg aaggccatga cggaaggagg cgtttacggt tcgactcccg
     4681 tcggggggtt accgtggggt atcgaggaca accggatatc cgcgaacgcg acgtgtttta
     4741 ccggcgccct ggacgccctc gggttcctgg tggcctctgg tagatgcgac cttggaataa
     4801 tcggcgccgc acaggtcgac aagttcggaa acgtcaacac caccggcttc tgggaagacc
     4861 cgacaagccc tcacttcggc ccgccgaaga ccaggcttac cgggagcggc ggggccaacg
     4921 acatcgcctg cggttgtaag cgcgtcgtca tcatgctggc gcacgagaaa aaaaggtttg
     4981 ccgaaaaggt ggattacatc acgagcccgg gatacctcga cgggggcaat gcccgcgaga
     5041 agtaccattt cgtgggcggc ggtcccgtag ccatcgtcac gacgctcgga atcctcaggc
     5101 cagacccggt gacgaaggaa atgctcatcg actcctattt ccccttttcc agcgtggagg
     5161 agatgaaggc gaacaccgcc tgggacctca aagtctcacc tggtgtgaag gtgactccgg
     5221 agccgacggc ggaagaattg aaggtcctgc gtgccgtgga cgtcacaaac gtacttaaga
     5281 gaacttagat tccggtggtc cggggtcgaa aacaaacagg aggtgttata tggctttgaa
     5341 gacaggcgac caatacgtgg aaagcataaa gaagatgcgg cccaacgttt acaaatgggg
     5401 cgagctcatc cgggatgtga cgacccatcc cgccacccgc ctccatatcc agtcggtgaa
     5461 acgctcttac gacctttcca acgacccggc gaaggcctcc atctatacgg cgaagtcaca
     5521 cctgacgggc gaggtggtcc atcgctggaa ttccctgatg cagtcggcgg aagacgtgat
     5581 gggtaactcc aacatgaagc gggagcagta tcaccagtcg ggaacctgtc agggtgcgac
     5641 ctgtgcgggg tggacggcga tgaacgtact ctgggacgcg acgtggcaga tggacaaaga
     5701 cctgggcacc gactaccacg ag
//
LOCUS       DAOTMX010000087         5580 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_12262_length_5580_cov_4.573756, whole genome shotgun sequence.
ACCESSION   DAOTMX010000087 DAOTMX010000000
VERSION     DAOTMX010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5580
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12262_length_5580_cov_4.573756"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="P5573_03550"
     CDS             complement(<1..227)
                     /locus_tag="P5573_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRT27173.1"
                     /translation="MPIYEYRCDKCGKEFEVFQGITEAPVKTCRFCQGPVKRLISLTT
                     FHLKGTGWYVTDYGGKKAPAAESAREVKASP"
     gene            complement(263..784)
                     /locus_tag="P5573_03555"
     CDS             complement(263..784)
                     /locus_tag="P5573_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27174.1"
                     /translation="MSQISGVNKLFGCAEELVGRFFRLSPDEVRANRYEVGTLATLQR
                     HELTDSAFAHLCKYYYQKAADEKDPSNFHFYRICLQDDRILDAVKRARSFIKLAPLML
                     YIATHELVHIIRFNRGYSEFDVSTEAKIVEEQRVDSITREILKPAADTDMTLVMDCFS
                     SKYKIDALYFNES"
     gene            complement(907..1434)
                     /locus_tag="P5573_03560"
     CDS             complement(907..1434)
                     /locus_tag="P5573_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27175.1"
                     /translation="MSSIEIDDALSSTRPSTTSSKSDFEDNWKFFGTLGAKGFYPFNE
                     IFGIGAFVQGTYFFRDADDNVSGTTGGAPYTMELRMTDLWDVNFGIGFQATLWRDTKL
                     YAGPYVYYAEVQVSPAPVISGIQLANGDTTLRNKTNVGGFMGIDVPIAKGFRLNVEGQ
                     YSERFSAGAAISLVY"
     gene            complement(1710..2669)
                     /locus_tag="P5573_03565"
     CDS             complement(1710..2669)
                     /locus_tag="P5573_03565"
                     /inference="COORDINATES: protein motif:HMM:NF016236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HRT27176.1"
                     /translation="MRAVKYLYSGEMMKRFYLLMFLIALMWAGYSHGADVAPTTHEAM
                     VSGQARSVIAASSSVQENKVGIGDVRASAVVGESPDEEAIDEEEEDEEEVMGEISIAD
                     PLEPFNRAMFQFNDRLYFWVVKPVAQGYKKVVPESGRTSVKNFFTNLGFPKRFVSCLF
                     QADFGGAATELGRFVVNTVWGVGGLLDPAANKELDLQRQDADIGQTLGIYGLGHGFYI
                     VWPVLGPSSLRDTVDIAGEYFQYPFTYADPWYVGPSVRAYEGVNSASLRIGDYEALKE
                     AAIDPYVALRNAYAQYRQNMVKKKEKREAPAPVEVKPAQRMFQ"
     gene            complement(2721..3296)
                     /locus_tag="P5573_03570"
     CDS             complement(2721..3296)
                     /locus_tag="P5573_03570"
                     /inference="COORDINATES: protein motif:HMM:NF017319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRT27177.1"
                     /translation="MKRCFVGFIALFVFVLPLTAHAGAPSDAINDTANKVIAILKNKS
                     LSAAAKKERLEVIYKRMFDEVELSRLSLGRNWNRLNPSQQQEFVQLFRKILEKAYGDK
                     ILEYKDEKVKITGERMISADRAEVQTKVIGSSKEIPVFYRLRLKDGEWKVYDVVIENV
                     SLVQNYRAQFTDILAKNSPSQLIEILRKKVQ"
     gene            complement(3293..3775)
                     /gene="mlaD"
                     /locus_tag="P5573_03575"
     CDS             complement(3293..3775)
                     /gene="mlaD"
                     /locus_tag="P5573_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HRT27178.1"
                     /translation="MKKYTMETTVGIFVVIGLLAIAYMTVKLGHVSFLVEDTYPLYAK
                     FTTVAGLRPGNYVEMYGIEVGRVERLTMDQENGLAVVELRINNGVKVYGDAVVSIKTE
                     GLIGDRYVHIDPGGAAEELKPGETITETQAPVDILDLIGKYVFGGVKTSEEPKKEDKP
                     "
     gene            complement(3775..5025)
                     /locus_tag="P5573_03580"
     CDS             complement(3775..5025)
                     /locus_tag="P5573_03580"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRT27179.1"
                     /translation="MDAPLIEFKDVTKRFGQLTVLDRVNAKIYEGDITTIIGLSGSGK
                     SVFLKHIIGLLKPEEGAVLFRGIPLDEMKKNEREASLAQISYMFQDNALFDSMTIYDN
                     VALPLRETTNLSRAQIDRRVMTRIEQMELGDAALKYPSELSGGMQKRAALARALVTDP
                     RIVLFDEPTTGQDPVRENAILSMVAENRKKFDFTAVLVSHAIPEVYFISNRILALYDR
                     KIVFQGPPEEFESFDHPLKEEIRRSLEELQERLSGLYAKRQFKVRYHTELKSGALQET
                     YAVLVFTLKDVDTLASSLGHDAVRKATDSMGSFIDSHFSTVGGFSARLGLDEFVTVLP
                     YSDVKEAEAILKGFADDFQERGIHDILYGKLEKVAAEMCFEFIVFAGIAEGRPIEGID
                     DIIESAKKEQREICRLRCRATGVG"
     gene            complement(5042..>5580)
                     /locus_tag="P5573_03585"
     CDS             complement(5042..>5580)
                     /locus_tag="P5573_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRT27180.1"
                     /translation="GAVGTLVALSLIRELGPVLTAIMITARAGSAITAEIGIQRISEQ
                     IDALDTMRIDPLRYLISPRIAASIVSFPLLTALFDLVGIVGGFISGVVLMGANAGTYF
                     YRVQASVDMKDVTDGFIKAVVFAFIVATVCCFQGYFVHTRTDSRGAKSVGLSTTSAVV
                     TSCVLILVADYVVTSLLI"
BASE COUNT         1258 a         1645 c         1357 g         1320 t
ORIGIN      
        1 tccggtgatg ctttcacttc cctggcactt tctgccgccg gtgccttctt tccaccgtaa
       61 tccgtcacat accaacctgt tcccttgaga tgaaaagtgg tcagggatat gagcctcttg
      121 acaggacctt ggcagaaccg gcatgtcttt accggcgcct cggttattcc ctggaagact
      181 tcgaattcct taccgcattt atcacacctg tattcgtata taggcatcgc acatacctcc
      241 caaaaatgat cggtaaactt ccttaagatt cattgaaata aagggcgtcg attttgtact
      301 tactactgaa acaatccatg accagcgtca tatcggtatc ggcggcgggc tttagaattt
      361 ctctcgtgat cgagtcgact ctttgctctt ccactatctt agcctccgtc gagacatcaa
      421 attcactgta tccccggttg aaacggatga tatggacgag ctcgtgggtg gcgatataaa
      481 gcatcagggg cgccaacttg atgaaagacc gtgcccgctt aaccgcgtcc agtatcctgt
      541 catcctgaag acatatccgg tagaaatgga agttagaagg atccttctcg tccgcggcct
      601 tctgataata atacttgcaa agatgcgcaa aggcgctgtc ggtcaattca tgcctctgaa
      661 gggtcgccag cgttcccact tcatagcggt tggcccttac ctcgtccgga ctcaacctaa
      721 aaaagcggcc gaccaactcc tctgcgcagc cgaacaactt gttaacgccg gatatctggg
      781 acatcgagaa aggcatcgcc aacctcatct caactatttc atagcccttc agtccatcaa
      841 aagtataatc atccttcagg gtttgtcaac gccaaacatc aaagtcaaag gcttttcggc
      901 atctgatcag tacactagcg atatcgccgc tccggcggaa aacctctccg aatactgtcc
      961 ttctacattc agacggaatc ccttggcaat gggcacgtcg attcccatga agccccccac
     1021 gtttgtcttg ttccgaagag tcgtatcccc gttagcaagc tggatcccgg aaatgaccgg
     1081 agctggagaa acctggacct cggcatagta gacgtaagga ccggcataca gttttgtgtc
     1141 ccgccacagc gtggcttgaa acccgatgcc gaaattgaca tcccataggt ccgtcatgcg
     1201 gagttccatg gtgtaaggag ccccgcccgt cgtcccggaa acgttgtcgt ctgcgtcacg
     1261 gaagaaataa gtcccttgca cgaaggcccc aatgccaaag atttcattga aaggataaaa
     1321 cccctttgcc cctaaggtac cgaaaaactt ccagttatct tcgaaatcgc tctttgaact
     1381 tgtcgtcgaa gggcgggtgg agctcaaagc atcatctatc tctatggatg acactccaat
     1441 ccggccgtac acctcccacg ttttccctgc tccataagcg acctcagaat agacttgatt
     1501 ctgtttgata acatggtccg tatcattact gaacttatcc tcgtggtacc agtagccaac
     1561 agctgtccga ataccgccgg agtcttttgt aagaggttgg ggtggcccga agggcccacc
     1621 tgcgtcagcc gtaccacaaa gaacgaccat catgaatatc aataagatta ttgcgccagg
     1681 atttattttc gccatgaacc cactcgattc tactggaaca tccgctgcgc tggttttacc
     1741 tcgaccggtg cgggagcttc tctcttttcc tttttcttca ccatattctg tcgatactga
     1801 gcataggcgt ttcggagcgc cacgtagggg tcgatggccg cctctttcaa cgcctcgtag
     1861 tcaccgatcc tcaaagaagc gctgtttacg ccctcgtatg ccctcaccga cggcccgaca
     1921 taccagggat cggcgtaagt aaaggggtac tggaagtatt caccggcgat gtcgaccgta
     1981 tcgcgcaaac tggacggccc taagacaggc cagacgatgt agaagccatg ccccaatcca
     2041 tagattccaa gggtttgacc gatgtcggca tcttgcctct gtaggtcaag ctccttgttc
     2101 gcggcagggt cgagcagtcc ccccacgccc catacggtgt tcaccacaaa gcggccgagt
     2161 tcagtcgctg caccgccaaa atccgcctgg aacaggcaac tgacgaagcg tttcgggaac
     2221 ccgaggttgg tgaagaaatt cttcacgctg gtccggccac tttcgggaac taccttctta
     2281 taaccttgcg ccactggttt cacgacccag aaatacagcc tgtcattgaa ctggaacatc
     2341 gcccggttga aaggctccaa ggggtcggcg atggagattt cgcccataac ctcctcttca
     2401 tcctcttcct cttcatcgat tgcctcctca tcggggcttt ccccaaccac tgcgctcgcg
     2461 cgcacatccc cgatacccac cttattttct tggacgctcg atgaagcagc aatcaccgaa
     2521 cgagcttgac cggacaccat ggcctcgtgc gttgtcgggg caacgtcggc gccgtgagag
     2581 taacctgccc acataagggc aatcagaaac atcagcaagt aaaaccgttt catcatctct
     2641 cccgagtaaa gatacttcac tgcccgcact caccgcaaca aaaatcgcgt cgattcctcg
     2701 gctcccttcc tccgaccccg ctactgtacc tttttccgca gtatctcgat cagttggcta
     2761 ggagaattct tcgccaggat atccgtgaac tgggcgcgat aattctgcac caggcttaca
     2821 ttctctataa cgacatcgta aaccttccac tctccatcct ttagtctgag ccggtagaaa
     2881 acggggattt ccttcgacga gccgatgacc ttggtctgca cctcagccct gtccgccgaa
     2941 atcatcctct ccccggtaat cttgaccttc tcgtccttgt actcgagtat cttgtcgccg
     3001 taagcctttt ccaatatctt ccggaaaagc tgcacgaact cctgttgctg ggaggggttc
     3061 agcctgttcc agtttcgccc gagggaaagt cgggaaagtt ctacctcgtc gaacattctc
     3121 ttgtatatga cctcgagccg ctctttcttc gcggcggcgc tgagagactt gttcttgagg
     3181 atggcgatca ccttgttggc ggtatcgttg atggcatccg acggtgcccc ggcatgagcc
     3241 gtaagaggaa gcacaaagac gaacaaggca ataaacccga caaaacacct tttcatggtt
     3301 tatcctcttt cttgggttct tcggacgtct tgacaccccc gaacacgtac ttcccgatga
     3361 gatccaggat atcaacgggc gcctgtgttt ctgtgatcgt ttctccgggt ttcagctctt
     3421 ccgcggctcc gccggggtcg atgtggacgt acctatcgcc gatcagaccc tccgtcttga
     3481 tggagacgac ggcgtcgccg tagaccttga caccgttatt gatcctcaac tccaccacgg
     3541 ccaacccgtt ttcctgatcc atagtgagac gctccacccg ccctacctcg atgccgtaca
     3601 tttcgacgta atttccgggt ctcaggccgg ccaccgtcgt gaatttggcg taaagagggt
     3661 atgtatcttc gacgagaaaa gagacatggc caagcttgac cgtcatgtac gctatggcaa
     3721 gcagtcctat caccacaaag atgcccaccg tcgtttccat ggtatatttt ttcatcaacc
     3781 cacccctgtc gcacggcagc gaagccggca tatttctctc tgctctttct ttgcagactc
     3841 tatgatgtcg tctatccctt cgatgggtct gccctcggcg attccggcga agacgatgaa
     3901 ctcgaaacac atctccgcag cgactttctc caatttcccg tataagatgt cgtggatacc
     3961 ccgttcctga aaatcatcgg cgaagccctt cagtatggcc tcggcctcct tcacatccga
     4021 atagggaagc actgtgacga attcatccaa gccgaggcga gcggaaaatc cgccgacggt
     4081 gctgaaatga ctgtcgataa aggagcccat gctgtctgtg gccttacgca ccgcatcatg
     4141 acccaggctg gatgcgagcg tatctacgtc ctttagagta aaaaccagga cggcgtaagt
     4201 ctcctgtagc gccccgcttt tcagttcggt gtgatagcgg accttgaact gccgcttggc
     4261 atatagtccc gaaagtcttt cctgcagttc ctccagactg cggcgtattt cctcctttaa
     4321 ggggtggtcg aaactttcaa attcctccgg gggaccctga aagacaatct tgcggtcgta
     4381 gagggcgagt atccggttgg agataaaata cacctcgggg atagcatgac tgaccagtac
     4441 cgccgtgaag tcgaattttt ttcggttttc ggcgaccata ctcaggatcg cattttccct
     4501 aacggggtcc tgaccggttg tgggctcatc gaaaagcacg atacggggat ccgtgaccaa
     4561 ggcccgcgcc agggcggccc gcttctgcat cccccccgac agctccgaag ggtacttcaa
     4621 tgccgcgtcg ccgagctcca tctgctcgat cctcgtcatc accctgcggt cgatctgtgc
     4681 cctgctcagg ttggtcgtct cccggagcgg caaggcgacg ttgtcataga tcgtcatgga
     4741 atcgaaaagg gcgttgtcct ggaacatata actgatctgc gcgagactgg cttcccgctc
     4801 attctttttc atctcgtcga gcggtattcc tcggaaaaga accgcccctt cttccggttt
     4861 gagaagcccg atgatgtgct taaggaagac actcttgccg gaaccgctga ggccgatgat
     4921 ggtcgttata tcgccctcgt aaatcttcgc gttcacccgg tcgaggacgg tcaactgtcc
     4981 gaagcgcttc gtcacatcct tgaattcgat caacggggca tccatcagtc cggcctattg
     5041 cctaaatcag gagagaagta acaacataat ccgctacgag gatcaacacg caggacgtta
     5101 caacggcgga ggtcgtcgaa agcccgaccg acttggcccc gcggctgtcc gtacgcgtgt
     5161 ggacgaaata tccctggaaa cagcaaacgg tcgccacgat gaaggcgaac accaccgcct
     5221 tgatgaaacc gtcggtgaca tccttcatat cgacgctcgc ttgaacacga taaaaatatg
     5281 tgccggcgtt agcacccata agaacgaccc cggagatgaa gcctccgacg atcccgacca
     5341 ggtcgaagag cgccgtcagc aaggggaagc tcacgatgga agctgctatc cgcgggctga
     5401 tcaggtaccg gagtgggtct atgcgcatcg tgtccaaggc gtcgatctgc tcggagatgc
     5461 gctggattcc tatttccgcc gtgatggcgg agcccgcccg ggccgtgatc attatggccg
     5521 tgaggacagg gcccagctcc cggatcaggg aaagagccac cagggtgccg accgcgccca
//
LOCUS       DAOTMX010000088         5516 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_12427_length_5516_cov_3.776598, whole genome shotgun sequence.
ACCESSION   DAOTMX010000088 DAOTMX010000000
VERSION     DAOTMX010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5516
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12427_length_5516_cov_3.776598"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="P5573_03590"
     CDS             complement(<1..735)
                     /locus_tag="P5573_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRT27181.1"
                     /translation="MAMEEKIFYVTTPIYYVNASPHIGHAYTTIVADVMARFYRLSGY
                     RTFFMTGTDEHGDKIAEAARKANTTPKSYADMVSAQFRELWPQLAITNDYFIRTTDEN
                     HVKTVQLILKKVYDAGDIYFGKYEGYYCVGCERFYMETELVDGKCPDHQVEPEFRQEN
                     NYFFRMSRYQDWLIDYIKGKPDFIRPERYRNEVLAFLREPLEDLCISRPKSRLEWGIT
                     LPFDDDYVTYVWFDALINYISGIGYPY"
     gene            complement(745..1566)
                     /locus_tag="P5573_03595"
     CDS             complement(745..1566)
                     /locus_tag="P5573_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HRT27182.1"
                     /translation="MKNVVGVRFNKQGKVYTFDATDQPLIKKGDRVIVETENGPAVGN
                     VVTAVRTVSEERLPTDLKKVIRRVSPEDQGIEERNRLVEKEAKAFCLERIRERNLPMK
                     LVEVECLFDRSKIVFYFTADNRVDFRELVKDLVQRFKTRIELRQIGVRNEARIFGGVG
                     ICGRELCCVGFLQNLDTVSVKMAKEQNMLLNPEKISGLCGRLMCCLAFEHEAYVSMKK
                     SVPKVGKSIDTPAGRGEVVRHNIVKGTVSVKVEDGKEVEVKVEDLQKGDSSRHPL"
     gene            complement(1634..2617)
                     /gene="holB"
                     /locus_tag="P5573_03600"
     CDS             complement(1634..2617)
                     /gene="holB"
                     /locus_tag="P5573_03600"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HRT27183.1"
                     /translation="MSFKDLRGQESQITLLRNAIEKDRLSHAYLFFGMKGVGKRTTAE
                     VFAKALNCAEGQIDACDRCISCLKIEKSNHPDVMVLKAAGQFIRIQEIREIRNQMGFR
                     PFEGRKRVFILVDAETMNIAAANALLKTLEEPSPSNILILLSSRPHRLPATIISRCQK
                     VRFRPLAREDVAGYLREKMGLGEEEADLLAASAGGSIGRAVEMQRDSELQARERLIAG
                     ISRCRKDDPMGLLLMAGALGKEREAILKSLDILRSWYRDVLVYSKTGGGENMIHRNHL
                     ETVKTFAEKLTTADVLKNVTAINRAVRAVERNANKHLTLETLLFKLVHPDG"
     gene            complement(2626..3279)
                     /gene="tmk"
                     /locus_tag="P5573_03605"
     CDS             complement(2626..3279)
                     /gene="tmk"
                     /locus_tag="P5573_03605"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HRT27184.1"
                     /translation="MGCFITFEGIEGCGKTTQIRMAGEFLEAKGIPVIVTEEPGGTAL
                     GAELRKLLLNRSDFEFCTESELFLFLSDRAQHVKELIMPALSRNMWVLCDRFTDATVA
                     YQCFGRGLDRDVVRGMVDFAAAGLRPRLTFLFDLPVETGLARAMDRGSKTGYASEADR
                     FESEKVEFHRRVREGYLVLAGEEPERFFVIDGRKEIPAVQREVSSRLTKLIDARNDG"
     gene            complement(3387..3560)
                     /locus_tag="P5573_03610"
     CDS             complement(3387..3560)
                     /locus_tag="P5573_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRT27185.1"
                     /translation="MAYTITDECIACGSCESECPVEAISEGDEKYVIDEKLCTDCGAC
                     ADICPVEAIMPGP"
     gene            complement(3609..3830)
                     /locus_tag="P5573_03615"
     CDS             complement(3609..3830)
                     /locus_tag="P5573_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HRT27186.1"
                     /translation="MKEKVQKAINDVRPNLQADGGDVQLVDVSDDGIVKVRLVGACAG
                     CPMSQMTLKAGIEKYLKQMIPEVKSVVSV"
     gene            complement(4119..4517)
                     /locus_tag="P5573_03620"
     CDS             complement(4119..4517)
                     /locus_tag="P5573_03620"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HRT27187.1"
                     /translation="MWTSTDLNDYVDFPYCGQVACIERHTEYLKSGKTRSEIAYLITS
                     LSPQKATPEQLLALNRGHWSIENKNHYVRDVTFDEDRSQIRTHTGPRIMATLRNLAIN
                     ILRTAGFTNIAQGIRSMAAKPYRALSLIGA"
     gene            complement(4558..>5516)
                     /locus_tag="P5573_03625"
     CDS             complement(4558..>5516)
                     /locus_tag="P5573_03625"
                     /inference="COORDINATES: protein motif:HMM:NF033564.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HRT27188.1"
                     /translation="ILSLNVKRLSDDWHRIHGHPVLVAETFVDTARFTGACYRAANWQ
                     YLGHTRGYGKHTRTYIYHGQPKAVFVFPLRKDALDHLRNPTPFKRKKPTMHVSQTQVD
                     DLINILKTLPDPRHKRGIRHRNLSLIALAVCAVVCGCRSYAAIAQWAAQCSQKMLERL
                     WCRKKEKRYIPPSEPTLRRQLQSIDADAVDRAISSWVSRLSSGNAIGVDGKTLRGAHR
                     PDGTRVHLLSAFVHQEGITIAQREVPAKTNEIPEAKPLLAPLELKGKVVTADAFHTHE
                     NLARFLVEDKHADYCFTVKNNQATLKDAIAYLGLNEDFPPSS"
BASE COUNT         1141 a         1572 c         1458 g         1345 t
ORIGIN      
        1 gtaggggtag ccgatcccgg agatgtagtt gatcagggcg tcgaaccaga cataagtcac
       61 gtagtcatcg tcgaagggga gcgtgatgcc ccattcgagc ctcgatttgg gccgggagat
      121 gcagagatcc tccaggggtt cccgcaggaa ggcaagtacc tcattgcggt aacgctcggg
      181 ccggatgaaa tcgggcttgc ccttgatgta gtcgattaac cagtcctggt agcggctcat
      241 ccggaaaaag tagttgtttt cctgacggaa ttccggttcc acttggtggt cggggcactt
      301 gccgtcgacg agctcagtct ccatgtagaa ccgctcacat ccgacgcagt aataaccctc
      361 atacttgccg aaatagatgt cgccggcgtc gtagactttt ttcaggatca actggaccgt
      421 cttcacgtgg ttctcgtcgg tcgtcctgat gaagtaatcg ttggtgatcg ccagctgcgg
      481 ccagagttcc cggaactggg cgctgaccat gtccgcgtag gacttggggg tggtgtttgc
      541 ctttctggcc gcttctgcta tcttgtcgcc gtgttcatcc gtccccgtca taaaaaacgt
      601 cctgtagccc gagaggcggt aaaatctggc catgacatcg gcaacgatcg tcgtgtatgc
      661 gtgaccgata tggggagagg cgttgacgta ataaataggt gtcgtgacgt aaaagatctt
      721 ttcttccatg gccatacctt tctcttagag cggatgccgc gacgaatccc ccttctgtaa
      781 gtcctcgacc ttgacctcga cttccttgcc gtcttcgacc ttcactgaaa cggtcccctt
      841 gacgatgttg tgtctgacga cctcaccgcg tccggcaggc gtgtcgatgc tcttgcccac
      901 cttcgggacc gactttttca tgctgacgta agcttcatgc tcgaaagcaa gacagcacat
      961 gagcctgccg caaagacccg atatcttttc ggggttgagg agcatattct gctccttcgc
     1021 catctttacc gacacagtgt ccaggttttg caggaatccg acgcaacaga gctctctacc
     1081 acatatgccc accccgccga agatcctcgc ctcgttcctg accccgatct gccgcagctc
     1141 tatccgggtc ttgaaccgct ggacaaggtc cttcacgagc tcccggaaat caacccggtt
     1201 gtctgccgtg aaatagaata cgatcttcga cctgtcgaat agacactcca cttccaccag
     1261 tttcatcggc aggtttctct cccggatgcg ttcaaggcag aatgccttgg cctccttctc
     1321 gaccagccgg ttcctttctt cgattccctg gtcctccggc gagaccctgc ggataacctt
     1381 cttcaggtct gtgggcagac gctcctcgga cacggtcctc accgccgtca ctacgtttcc
     1441 cactgccgga ccgttctccg tctcgacgat gaccctatcc ccctttttaa tcagaggctg
     1501 gtccgtcgca tcgaaggtgt atacttttcc ctgtttattg aaccttacgc cgacaacgtt
     1561 cttcatcttc tatcgcgatt cttcccgtgc gccttcgagg ccggcccacg gtgtctattc
     1621 aaactacgtc tgttcatccg tcgggatgca ccaatttaaa cagcagggtt tccaatgtca
     1681 aatgtttatt ggcattacgc tcgacggcgc ggacggcccg gttgatggcc gtgacattct
     1741 tcagaacatc cgctgtcgtg agtttttctg cgaaggtctt caccgtctcc agatggttcc
     1801 gatgaatcat gttctctccc ccacccgtct tgctgtaaac caacacatcc cggtaccagc
     1861 ttctcaggat gtcgagactc ttaaggatgg cttcccgctc cttacccaag gcgccggcca
     1921 tcaaaagcag tcccataggg tcgtccttac ggcaccggga aatgccggcg atcagccgct
     1981 ctcgcgcctg gagctccgaa tccctctgca tctctacagc cctcccgata cttccccccg
     2041 ccgatgccgc cagtaaatcc gcttcttcct ccccgagacc catcttctct ctgagataac
     2101 cggcgacatc ttcgcgggcg aggggacgga agcgaacctt ctggcaccgg gatatgatcg
     2161 ttgcgggaag ccggtgggga cgcgagctca ggagaatcag gatattcgac ggagacggtt
     2221 cttccagggt tttcaggagg gcgttagccg ctgcgatgtt catcgtctcc gcgtcgacga
     2281 ggataaaaac cctctttctc ccctcgaacg gtctgaaccc catctggttc cggatctccc
     2341 ggatctcctg tatccggatg aactgacccg cggctttgag aaccatcacg tcaggatgat
     2401 tgctcttttc gattttcaag caggagatgc acctgtcgca ggcgtctatc tgaccctcgg
     2461 cgcaattcag ggccttcgca aaaacctccg ccgtcgtcct cttgcccaca cccttcatcc
     2521 cgaagaagag gtaggcgtgg gaaaggcggt ctttttctat ggcattccga agaagggtta
     2581 tctgcgattc ctgcccccgg agatccttga aggacatggc tcgcctcaac cgtcatttcg
     2641 tgcatcgatg agctttgtga gacgggaaga aacctcacgc tgaacggccg gaatttcttt
     2701 tcgcccatcg atcacgaaaa acctttccgg ttcctccccg gcaaggacaa gatagccttc
     2761 cctgacccga cggtgaaact caaccttctc gctctcgaaa cgatctgcct ccgatgcgta
     2821 ccccgtcttt gagccccggt ccatggccct tgccagcccc gtttcgacgg ggaggtcgaa
     2881 aagaaaggtt agacggggcc tcagaccagc ggcggcaaaa tccaccatcc ccctgacgac
     2941 gtcccggtca aggcctctgc caaagcattg ataagccact gtggcgtcgg taaaacggtc
     3001 gcagaggacc cacatattcc ggctcagggc cggcattatc aactccttca catgctgggc
     3061 cctgtcggaa aggaaaagaa agagttccga ctcggtacaa aactcaaagt ccgaccggtt
     3121 caggagaagc ttcctgagtt cggcaccgag ggcggtaccg ccgggttcct ccgtcactat
     3181 caccgggatg cccttcgctt ccaggaactc tccggccatc cgtatctggg tcgtcttccc
     3241 gcagccctca atcccctcga aagtgatgaa acatcccatt tttccgcctt ctgaagtcta
     3301 aaaaaaccgg ccccggtgtg gggaaaaaga ttggggaacc accttttgga gggaagtgat
     3361 tccccaatct tttgcggcct caaaaactac ggaccgggca tgatcgcttc gacggggcag
     3421 atatcggcac aggcaccgca atcggtacac aatttttcat cgattacgta cttttcgtcc
     3481 ccctcactga tcgcttccac cggacattcg ctttcacaac tgccgcaggc gatacactca
     3541 tctgtgatcg tataagccat ttctgcaaaa acctccgata atcttttagc tgctgaaacc
     3601 gctcttcact acacggatac aaccgacttg acctcgggga tcatctgctt caggtatttc
     3661 tcgatccccg ctttcaacgt catctgggac atcgggcagc cggcacaggc cccgacgaga
     3721 cgcaccttca cgataccgtc atcgctcacg tccacgagct gaacatctcc gccgtcggcc
     3781 tgaagattcg gcctcacatc attgatcgct ttttgaacct tttccttcat gatttttcct
     3841 cccttttctc tccccgcaag ggaatccggt ctgaataaac gttgttctat agcacatctc
     3901 gacacctttc gccaccctct tccataaaac agcagggacg cgtcaaagtc acgggagtat
     3961 ttttgaggaa ggtaggtgga ttttcacatt ggactgagtg atgggggaga ataaggcgag
     4021 acggagcgag gaagggatgt tcgcatcagg ggatagtcag aggaagtcgt tgcgtcacgg
     4081 ctacgatgcg tgcgggagga tggcgagggg aatgatgtct atgcaccgat tagtgacagg
     4141 gcacggtaag gtttggcggc catagagcgg attccttggg cgatattggt gaatccggcc
     4201 gttcgcaaga tgttgatggc aaggttgcgc agggtagcca taatccttgg gccggtatgg
     4261 gttcgtattt gggaacggtc ttcatcgaag gtgacgtccc ggacataatg atttttattt
     4321 tcgatgctcc aatgtcctcg attgagggca aggagttgtt ccggggtggc cttttgggga
     4381 gataagctgg taataaggta agcgatttca gagcgcgttt tgcctgattt gaggtattcc
     4441 gtatggcgtt cgatgcaggc gacctgtccg caatagggga aatctacata gtcattgaga
     4501 tcggtagagg tccagatttt tctggtttcc aggcggccat ggcccttgtc caggatttca
     4561 tgatgagggg ggaaaatcct cgttcaggcc gaggtaggcg atggcatctt tcagcgttgc
     4621 ctgattgttc ttgacggtaa agcagtaatc ggcatgtttg tcctcaacga ggaagcgggc
     4681 gagattctca tgggtgtgga aagcgtctgc ggtgacgact tttcccttca actccagtgg
     4741 tgccaacaat ggttttgctt cagggatttc attcgtcttg gcagggactt ctctttgggc
     4801 aatcgtgatg ccttcctggt ggacaaaagc agaaagcagg tgcacccggg tcccatcagg
     4861 gcggtgtgct ccgcggaggg ttttcccgtc cacgccgatc gcattccccg aggacagacg
     4921 ggacacccag gaggaaatgg ctctatccac tgcgtctgcg tcgatgcttt gcaattgccg
     4981 gcgcagggtg ggttcgctgg gagggatata cctcttttct tttttcctgc accacagacg
     5041 ctccagcatc ttttgcgaac actgtgctgc ccactgggcg atcgccgcat aactgcggca
     5101 tccgcacacg acggcgcaca cggccaaggc aatgagggag aggtttctgt ggcgaatccc
     5161 tcgcttgtga cggggatcag gaagtgtttt caagatgttg atcaaatcat ctacctgtgt
     5221 ctgtgaaacg tgcatggtcg gcttctttct cttgaaaggg gttggatttc tgaggtggtc
     5281 aagggcatcc ttacggagag gaaacacaaa aaccgctttc ggttgcccat gatagatgta
     5341 cgtacgggta tgttttccgt accctcgcgt gtgccccaga tactgccagt tcgccgccct
     5401 gtaacacgca ccggtaaaac gggccgtgtc cacaaaggtt tctgccacga gcactgggtg
     5461 gccgtgaatg cgatgccaat cgtcagaaag acgctttacg ttgaggctca gaatct
//
LOCUS       DAOTMX010000089         5509 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_12447_length_5509_cov_3.758343, whole genome shotgun sequence.
ACCESSION   DAOTMX010000089 DAOTMX010000000
VERSION     DAOTMX010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5509
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12447_length_5509_cov_3.758343"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..178
                     /locus_tag="P5573_03630"
     CDS             8..178
                     /locus_tag="P5573_03630"
                     /inference="COORDINATES: protein motif:HMM:NF012991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27189.1"
                     /translation="MARAAVAAGVDGVFLEVHPDPDTALCDGPNSLRLDTLPRLLRLL
                     QEIDNVVKGARL"
     gene            175..717
                     /locus_tag="P5573_03635"
     CDS             175..717
                     /locus_tag="P5573_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HRT27190.1"
                     /translation="MRRGMSKDLKDRWKNISLLILDVDGVLTDGRIIMSDDGKEWKQF
                     DVKDGHGIKMLLRSGVGVVLLTGRSSRVVAHRARDLGVVDVFQGARDKVRVFDEVLWS
                     KGVEAEHVAYMGDDVVDVPLFKRVGIAIAVADACREARKAADYVTEREGGHGAVREVC
                     DLILRARGDWKKIIAQYEIS"
     gene            714..1391
                     /gene="lptC"
                     /locus_tag="P5573_03640"
     CDS             714..1391
                     /gene="lptC"
                     /locus_tag="P5573_03640"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HRT27191.1"
                     /translation="MTPDESVFYHFVYMVGLGCYTSADTMGNNPSKIVRWMFTGRRRT
                     FYVGIVFVVILAAALIYLLRDGRNGDATREVKLTIIPDQADVQIKDIHFTEVGDPELT
                     WEIHADTARYRKKENLVSFERVRIKLTRKNGKSLTLTGNEGVLHTDTKDAEIFGNVVA
                     SSSGGDVVETDRLYYSDKERKIFTDKKITLRNERMQVDGTGMELSFPEERVTLQSRVK
                     AVIKAGR"
     gene            1396..1971
                     /locus_tag="P5573_03645"
     CDS             1396..1971
                     /locus_tag="P5573_03645"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HRT27192.1"
                     /translation="MNISLLRRLSEVTVVVLLTAFFIQSAGAADRSGSKAVSLEGKGP
                     IEITSDRLDAYNEEKLVVFSGNVVATQADKVIRADQLFVYYKKKKGTKGQKSKDFGQE
                     AGELDRIEARGNVQVTQGKKVVTGEHGVFYNDEQKIVMTGNPVMREGDNILKGERIIV
                     LLDEDRGIVESSKEKRVTATIYPDESKNKKR"
     gene            1992..2714
                     /gene="lptB"
                     /locus_tag="P5573_03650"
     CDS             1992..2714
                     /gene="lptB"
                     /locus_tag="P5573_03650"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HRT27193.1"
                     /translation="MEKLLTHRLTKIYGKRRVVNEVDLEVRQGEVVGLLGPNGAGKTT
                     TFYMIVGLIKPDDGNIYLNGEDISLKPMYLRARKGINYLPQEPSIFRKLTVEENIMAI
                     LETLSIDRYEREQRLRELLGELDLTDLAKNKAYSLSGGERRRVEITRALVTSPKYIFL
                     DEPFAGIDPLAVADIQKIIGKLKTKGIGVVISDHNVRETLSVCDRAYIINEGSVLVEG
                     NPDSIAKSEIARRIYLGEDFSF"
     gene            2770..4224
                     /gene="rpoN"
                     /locus_tag="P5573_03655"
     CDS             2770..4224
                     /gene="rpoN"
                     /locus_tag="P5573_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HRT27194.1"
                     /translation="MAFELKQNLKLAQQLIMTPQLQQAIKLLQLSRVELVEAVQQELE
                     ENPMLEEVTGDDESEDDLQPEVEEIRAAERPAELTGEGDGKEEFDWDSYLEDYGPVGV
                     SYQREEVEGPTWENILSWKPSLIDHLSWQLRLSKLSELEVRVGEQILGNIDGNGYLTS
                     SVEEIAEAEKVDTALVEGVLKKIQEFDPPGVAARDLKECLLIQARVLGVETPLIEQIL
                     TNHIKDLETKNYNQIARKLKVQLSEVLDAVAVISQMDPKPGIAYSEEREEYIVPDVFV
                     LKVGDEYKIVLNEDGLPKLRISNFYKEILGGVGGAKKAGCKEYIKDRIQSATWLIKSI
                     QQRQRTIYKVSESIVKFQKDFFDRGIQYLKPMILRDVAEDVGMHESTISRVTTNKYMH
                     TPRGIFELKYFFNSRIGMTDGESIASKSVQEEIRKIIQAEDKAKPLSDSELVDILRKS
                     GIAIARRTVAKYREMMGILPSSKRKVFTAKKKNS"
     gene            4294..4842
                     /gene="raiA"
                     /locus_tag="P5573_03660"
     CDS             4294..4842
                     /gene="raiA"
                     /locus_tag="P5573_03660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HRT27195.1"
                     /translation="MQVSVTFRNTEAEEWFKDYVNERLAKLKKYIDKPFEAHVIMTVE
                     KFRHVAEINLKAKGVNIVGKEEAKDMQLAFDAVMDKVERQVKKHREILRDHKAATVRK
                     PELVGKVSPQDETMAEGEEERGKVIETRKIVVEPMSLDDALLALEGSKKPFFIYRDLS
                     SENVSILYRRDDGNYALIETSH"
     gene            4855..5319
                     /locus_tag="P5573_03665"
     CDS             4855..5319
                     /locus_tag="P5573_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRT27196.1"
                     /translation="MNISGMIAKKYILEDLKSSRKQDVLSELTAVFGLDNDLEGNNQT
                     VKVLLEREKLGSTGIGEGIAIPHGKVPGIEKIYIGFGRSKTGVDFNSLDGRPVHLFFV
                     LLAPENSSSTHLKALAKLSRMLMDQPFREDLMKAESTDVIYSAIVDMDRRVI"
BASE COUNT         1530 a         1257 c         1634 g         1088 t
ORIGIN      
        1 gccgtacctg gcgagagccg ctgtggccgc cggagtagac ggtgtgttcc tcgaagttca
       61 tcctgacccg gatacggccc tttgcgacgg tccgaactcc ctccggctgg atacccttcc
      121 ccgcctcctc cgcctgttgc aggaaataga caacgtcgtg aaaggagccc ggctatgaga
      181 agaggaatga gtaaggacct caaagacagg tggaaaaaca tctctctgct catcctggat
      241 gtcgacgggg tcttgacgga cggccgtatc ataatgagcg acgacggaaa agaatggaag
      301 cagttcgacg tgaaggacgg ccatggtatc aagatgttgc tgagaagcgg cgttggggtc
      361 gtcttactga cgggcaggag ctccagggtg gtcgcccatc gggcgcgcga cctgggtgta
      421 gtcgacgtat tccagggggc cagggataag gtgagggtgt tcgacgaagt gctatggtcg
      481 aaaggagtgg aggcggagca cgtcgcttac atgggcgacg atgtggtgga cgtcccgttg
      541 ttcaaaagag taggcatcgc tatagccgtg gcagatgcat gccgagaggc ccggaaggcc
      601 gcagattatg tcacggaaag ggaaggcggt catggtgcag tcagagaagt ctgcgacctc
      661 atcctccggg cccgcggtga ttggaaaaaa atcatagcac agtatgaaat ctcttgaccc
      721 cggacgaatc agtcttctat cacttcgttt acatggtcgg ccttgggtgc tatacgtcag
      781 cggacacgat ggggaataat ccttcgaaaa tagtaagatg gatgttcact ggtcggagaa
      841 gaaccttcta cgtgggtatt gtctttgtcg tcatcctcgc agcggcgttg atttacctct
      901 tgagggatgg aaggaatggg gatgcgacaa gggaggtaaa actgacgata atccctgacc
      961 aggcggatgt acagatcaag gatattcatt tcaccgaggt aggcgacccg gagttgacct
     1021 gggagataca tgccgatacg gcgagatacc ggaagaaaga gaacttggtg agctttgagc
     1081 gcgtccgcat aaagctcacc cgtaaaaacg gcaagtcgct gacgttgacg ggaaacgaag
     1141 gagtgctgca tacggatacc aaagatgccg agatattcgg caacgtcgtt gcttcgtcca
     1201 gcgggggaga tgtggtggaa acagacagac tttactactc cgacaaggag cgaaaaatct
     1261 tcacggacaa gaaaatcacc cttaggaacg aacggatgca ggtggacggc accggcatgg
     1321 aactttcgtt tcctgaggag agagtgacac ttcagtcgcg cgtcaaggcg gtcataaagg
     1381 cgggaaggtg agaagatgaa cataagcctt ttaagacgtt tgtccgaggt aacggttgtg
     1441 gtgctgctga cggcattctt tatccagtcg gcgggtgccg ccgacaggtc cggcagtaag
     1501 gccgtgtcac tggaaggaaa aggtccgata gagataacat ccgaccgcct ggatgcctac
     1561 aatgaagaaa aactcgtggt tttttccggc aatgtcgtgg cgacgcaggc cgacaaggta
     1621 atccgcgccg accagctttt tgtttattat aagaaaaaga aggggacaaa ggggcaaaag
     1681 tcaaaggact tcggtcaaga ggcgggcgaa ttagacagga tcgaagcgag gggtaacgta
     1741 caggttaccc agggtaagaa ggtcgtgacc ggtgaacacg gcgtctttta caacgacgag
     1801 cagaaaatag tcatgacggg aaacccggta atgcgggagg gagacaacat cctcaaaggg
     1861 gaaaggatca tcgtccttct cgatgaggac cggggcatag tggagagctc gaaggagaag
     1921 cgggttacgg ccacgatata ccccgatgaa tccaagaata aaaaacgttg atccgccaaa
     1981 gtgggcactc gatggaaaag cttttaacgc accgtttgac caagatatac ggtaagagga
     2041 gggtcgtcaa cgaagttgac ctcgaggtga ggcagggaga agtggtcgga ctcctcggac
     2101 cgaacggcgc gggcaagacg acgacctttt acatgatagt cggcctgatc aagcccgacg
     2161 acggcaatat ttatctgaac ggtgaggaca taagccttaa gcccatgtac ctgcgggcta
     2221 ggaaggggat caattaccta ccccaggagc cctctatttt caggaaactg acggtcgagg
     2281 aaaacatcat ggccatactt gaaacccttt ctatcgaccg gtatgaaagg gagcagaggt
     2341 tgagagaact tctcggcgaa ctggacctga ccgaccttgc aaagaacaag gcctattccc
     2401 tgtcgggggg ggaaagaaga agggtcgaga tcacgcgggc gctcgtcact tcaccgaaat
     2461 acatcttcct ggacgagcca ttcgccggta tcgacccctt ggcggtagcg gatatacaga
     2521 agatcatagg aaagttaaag actaaaggta tcggcgtcgt catttcggac cataatgtgc
     2581 gggagaccct ttcggtctgc gatagggcat acatcataaa tgaagggagc gtccttgtcg
     2641 aaggcaatcc ggactccata gcgaaaagtg agatcgcaag gaggatttat ctgggagaag
     2701 acttcagctt ctgaacgttg cctactcacc acaaatagtg tatatcttgt cgtaaggaag
     2761 taacggaaga tggcttttga attaaaacaa aacctgaaac tcgcccagca gttgatcatg
     2821 acaccacagc ttcagcaggc gatcaagctg ctgcagctgt cgagggtgga gcttgtggag
     2881 gccgtccagc aagaactcga ggagaacccg atgctggaag aggtgaccgg ggacgatgaa
     2941 tcggaagacg acctccagcc cgaggtggag gagatacgag cggccgaacg gccggcagag
     3001 ctgaccggcg aaggggacgg caaagaggaa ttcgactggg acagctactt agaggattac
     3061 gggcccgtcg gcgtttctta tcagcgtgag gaggtcgaag gaccgacgtg ggagaacatc
     3121 ctttcttgga agccttctct catagaccac ctttcttggc agttgcgact ttcgaaactg
     3181 tccgaactcg aagtaagggt gggcgaacag atactcggga atatcgacgg taacggctat
     3241 ctgacgtcat cggtcgaaga aatcgccgaa gcggagaagg tggatacggc cttagttgaa
     3301 ggggtcctga agaaaataca ggaattcgac ccgcccgggg tcgcggcaag ggacttgaaa
     3361 gagtgcctcc tgattcaggc gagagtcctc ggcgtcgaaa ccccgctgat cgagcagatc
     3421 ctcaccaatc acatcaaaga cctggaaacc aagaattaca accaaatagc caggaaactg
     3481 aaagtccagc tcagcgaggt tctcgatgcc gtagcggtga tcagtcagat ggaccccaag
     3541 ccgggtatcg cttacagcga agagagggaa gagtacatag tacccgacgt gttcgtcttg
     3601 aaggtaggag atgagtacaa gatcgtcctc aacgaagacg gcctgcctaa gctgaggatc
     3661 agcaattttt acaaagaaat cctcggcggg gtcggcgggg ccaaaaaggc agggtgcaag
     3721 gagtacataa aggacaggat tcagtctgct acctggctca tcaagagcat ccagcagcgc
     3781 cagaggacca tatacaaggt gtccgaaagc atcgtcaagt ttcagaaaga cttcttcgac
     3841 cgcggcatcc agtacctcaa gccgatgatt ctcagggacg ttgccgagga cgtcggtatg
     3901 catgaatcga ctatcagcag ggttacgacg aataaataca tgcacacgcc gcgcggtatc
     3961 ttcgagttga agtatttttt caacagcaga atcggcatga cggacggcga gtcgatagca
     4021 tcgaaaagtg tccaggaaga gatccgcaaa atcatccagg ccgaggacaa agcaaaaccg
     4081 ttgagcgaca gcgaactggt agacatcctg agaaaatccg gcatagcgat cgccaggcgt
     4141 accgtcgcaa agtaccggga gatgatgggc atcttgccgt cttcgaagag gaaagtcttc
     4201 acggcgaaga agaaaaacag ttgactttca aacgatactg cattatatgg ccaaatttta
     4261 attttaggta atcagtcgcc aggaggggaa tacatgcagg tttccgtaac ctttagaaat
     4321 acagaggcgg aagagtggtt caaggattat gtgaatgagc gtttggcgaa attaaaaaaa
     4381 tacatcgata aacccttcga ggcccatgtt atcatgacgg tcgaaaaatt caggcatgtc
     4441 gctgaaatca acctaaaggc caaaggagtc aatattgtcg gaaaagaaga agccaaggat
     4501 atgcagttgg ctttcgacgc cgttatggat aaggtcgaaa ggcaggtaaa gaaacacagg
     4561 gagattttgc gggaccacaa agccgcaact gtgagaaaac cagaactcgt cggtaaggtc
     4621 tccccccagg acgagacgat ggcggagggt gaggaagaga ggggaaaggt gatcgagacc
     4681 aggaaaatag ttgttgaacc tatgtctctt gacgacgcct tattggccct tgagggttcg
     4741 aagaaaccct tttttattta cagagacctt tcatcggaaa acgtcagcat tctgtatagg
     4801 agggatgacg gcaactacgc gcttatcgaa acgagtcatt gacagcaggt agagatgaac
     4861 ataagcggca tgattgccaa gaaatacatc ctcgaagact tgaagtccag cagaaagcag
     4921 gatgtcctga gtgaattgac ggcggttttc ggcctggata acgatctcga gggaaacaac
     4981 caaacggtga aggtccttct tgaacgagag aagctcggca gcactgggat cggagaaggc
     5041 atcgcgatac cccacggtaa ggtccccggg atcgaaaaga tatacattgg ttttggaagg
     5101 agtaagaccg gggtcgattt caactctctc gacggaaggc ctgtacacct tttctttgtc
     5161 cttctcgcac cggaaaactc ctcgagcacg cacctgaagg cgctcgctaa attgtcgcgt
     5221 atgctgatgg atcagccctt cagggaggac ctgatgaagg ccgagtcgac tgatgtcatc
     5281 tattccgcca tagtagatat ggaccggcgc gtgatttgat tccggcagag acccggcttc
     5341 atgccctcgg gaaacgcgag cctgcatggg ttgttcatcc gttgaagggc cggccgggga
     5401 gatgagcggc gacatcatcg agataccacc taccgaaaag ggttcaatcc tcccgtggaa
     5461 tcaagagaag tccggagatt cgaaattttc ttgcccgacc ttctcgcgg
//
LOCUS       DAOTMX010000090         5462 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_12575_length_5462_cov_4.012946, whole genome shotgun sequence.
ACCESSION   DAOTMX010000090 DAOTMX010000000
VERSION     DAOTMX010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5462
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12575_length_5462_cov_4.012946"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /gene="murB"
                     /locus_tag="P5573_03670"
     CDS             <1..371
                     /gene="murB"
                     /locus_tag="P5573_03670"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF014872.2"
                     /note="catalyzes the reduction of UDP-N-acetylglucosamine
                     enolpyruvate to form UDP-N-acetylmuramate in peptidoglycan
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HRT27197.1"
                     /translation="VIIGAEFALHRASRERVRARVAEILAIRKSRHPLQYRSAGSVFK
                     NPASVPAGKLIDEAGLKGTRIGGAMVSDEHGNFIVNTGAATAKDVIALVELVESRVFE
                     KTGVRLEREVKIIGEEEGRS"
     gene            368..1210
                     /locus_tag="P5573_03675"
     CDS             368..1210
                     /locus_tag="P5573_03675"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HRT27198.1"
                     /translation="MKSRTGAGWKKSVEIRKNRLKRRSNKIFREAAVVFLLWAVVLVM
                     GVLLIFSYNLVLTSPWFRLEKTVVKGCANTTEQDLLLVSGVKPATNILALNVDGIARK
                     IKMNPWVEEVSVVRELPNRLIIEIQERKPIALVKREDSLYFMDGQGVIFKKFEGEEAN
                     LPVLTGCGEGGKEDPVLIGKSIELINLLSASNSFPQIKNVSEIHGEEVLGFSIFLDSG
                     LCLRVGFGDYEGKLKRLKSVLADLARRNVNPGFLTIDLNDPGKVTVQKHDLTGGSRGK
                     EVRT"
     gene            1260..2480
                     /gene="ftsA"
                     /locus_tag="P5573_03680"
     CDS             1260..2480
                     /gene="ftsA"
                     /locus_tag="P5573_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HRT27199.1"
                     /translation="MMGRKRKTIAGLDIGTTKVCVVVGEVTPGGVEIIGIGSSPSEGL
                     RKGVVVNIDNTMDSIRKAVEEAEKLCDCDIRRVYAGVSGSHIKSFNNQGMVVVKGNEV
                     EDADVQKVIEAAKAIPMPADREVLHILPQYYVVDDQDGIKDPIGMSGVRLEVNVHIVT
                     GAVSPVQNVVRSVNGVGLDINGVVLEQLAASEAVLSADEKELGVALVDIGGGTTDIAV
                     FSEGSVKYTTILPLGGGYLTNDIAIGLRTPVTEAEKVKIRYGCACTPMIPQDEMIEVP
                     SVGGREPRRVSRQVLGSIIEPRMEEILNLVCREIVKSGFEDRLAAGIVLTGGTAILEG
                     ITELAEHIFNMPVRRGIPIGVAGPDEIKSPIYATGIGLVLYGAKQSSTNGRNEGGLFR
                     GVMKRGRKWFSSIF"
     gene            2496..3662
                     /gene="ftsZ"
                     /locus_tag="P5573_03685"
     CDS             2496..3662
                     /gene="ftsZ"
                     /locus_tag="P5573_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HRT27200.1"
                     /translation="MFELCEGKGDRSARIRVIGIGGGGCNAVNTMISSNLTGVDFIAA
                     NTDAQSLKTSLSPTKIQLGVGITKGLGAGADPETGRRAAEESVDLIRSVLEDQDMVFI
                     TAGLGGGTGTGGAPIVAQVAKELGALTVAVVTKPFQFEGKKRERHAEDGIAELRGIVD
                     TLIVIPNQRLLSMGGRNMTLLEAFKKADDILYQAVKGVSDLILVPGYINLDFADVRTI
                     MSGMGLALMGTGLARGENRAVEAAQRAISSPLLEDNSIQGARGVLLNITGGPDMSLFE
                     INEASSLIQAEAHEDANIIFGTVIDESMEDEIRITVIATGFDESMARQQAAANVSHLS
                     SYKREDLSTPAFLRKGGKAAGAENLNVIKVGVIDDLEVDNADFEVPSFLRKKAD"
     gene            complement(3747..4421)
                     /locus_tag="P5573_03690"
     CDS             complement(3747..4421)
                     /locus_tag="P5573_03690"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - oxidoreductase activity, acting
                     on NAD(P)H, nitrogenous group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HRT27201.1"
                     /translation="MDIIEAIRKRKSIRGYRQDAVPREVIRDILETAVRAPSAMNTQP
                     WEFAVLSGQPLRAIGEECAEKFRAAEEPHPDHEVIGWTNNSVYRTRQVELAKHIFQVM
                     NIPREDKAKRAAWMERGFRYFDAPAAIIIMVDKALSAAAPLLDLGAVMQTICLAALHY
                     GLGTCIHDQGVMYPDVVRRHAGISEAKRLIVAISLGYPDEGNIANTITTSREPVDNIT
                     SWVGFE"
     gene            complement(4643..5086)
                     /locus_tag="P5573_03695"
     CDS             complement(4643..5086)
                     /locus_tag="P5573_03695"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRT27202.1"
                     /translation="MAGDIKEQVINYMEKCRACTIATATTDGQPNIATVFFKNAGADI
                     YFNTGKDSQKVRNIQTNPRLAIAMQEEGPIPKEDREIKGIQYLGKAQILAEKDYAEVP
                     KAVIARHNAFNSVKPGNSVVVKVTPVKIYLIDYSRGFRHRDVIEF"
BASE COUNT         1221 a         1412 c         1677 g         1152 t
ORIGIN      
        1 ccgtcatcat cggtgccgag ttcgctctcc acagggcgtc ccgggagcgg gtgcgggcgc
       61 gggtcgcgga gatcctggcg atacggaagt ccaggcatcc gcttcagtac cgtagcgccg
      121 gttccgtttt caagaacccg gcgtcggtgc cggcgggaaa gttgatagac gaggccggcc
      181 tgaagggcac gaggatcggg ggcgccatgg tttcggacga acacggtaat ttcatcgtaa
      241 acacaggcgc ggctacggca aaggacgtca tcgccttggt cgagttggtc gagagccggg
      301 tgttcgagaa aacgggcgta cgtctggaaa gggaagtgaa aatcattggc gaagaggaag
      361 gacggtcatg aagtccagga ccggggcggg ctggaaaaag tcggtggaaa taagaaaaaa
      421 tcgactgaag cgacggtcga acaagatctt ccgtgaagcg gccgtcgttt tccttctctg
      481 ggcggtagtc ctcgtgatgg gtgttcttct gattttctcc tacaacctcg tccttacgtc
      541 gccctggttt aggctcgaaa agacggtagt gaagggctgt gcgaacacaa cggagcagga
      601 cctgcttctc gtctcgggcg tcaagccggc gacaaacatc ctggccctca atgtggatgg
      661 gatcgcccgc aagatcaaga tgaacccctg ggtggaggaa gtctctgtag tccgtgaatt
      721 gccgaaccgt cttataatcg agatccagga acggaagccc attgccctgg tgaagcggga
      781 agatagcctc tatttcatgg acggtcaagg ggtgattttc aaaaagttcg agggagaaga
      841 agccaacctg cccgtgttga cgggctgcgg ggaaggcgga aaagaagacc cggtgttgat
      901 cggtaagtcc atcgagctga tcaaccttct gtccgcgtcg aacagctttc cgcagatcaa
      961 aaacgtgtcc gagatacatg gcgaggaagt cctgggtttt tccatctttc tcgacagcgg
     1021 tctttgcctg agagtaggct tcggcgatta cgagggtaag ttgaagaggc tgaagtcggt
     1081 cttggccgac ctggcgagga ggaacgtaaa tccggggttt ttgacgatcg acctcaacga
     1141 cccgggcaag gtcaccgtcc agaagcatga tcttaccggc ggttcgaggg gcaaagaggt
     1201 taggacttaa gacaggtcaa agttgtgagc aaaaaatcct ttgagctttt atcgggggga
     1261 tgatgggaag aaagagaaaa acaatcgcgg gccttgatat cgggacgaca aaggtctgtg
     1321 tagtggttgg agaggtgacg cccggcggtg tggagatcat cggcatcggc tcgtctcctt
     1381 cggagggttt gaggaagggt gtcgtggtaa acatcgataa cactatggac tccatccgaa
     1441 aagccgtgga agaggcggag aaactgtgcg attgcgatat ccgacgggtg tacgcgggtg
     1501 tctcgggcag tcacatcaag agcttcaaca accagggaat ggtcgtcgtc aaaggcaatg
     1561 aggttgaaga cgcagacgtg cagaaggtaa tcgaggccgc gaaagccatt cccatgccgg
     1621 ccgaccgcga ggtcctccat atcctacccc agtattacgt ggtggacgac caagacggga
     1681 tcaaggaccc catcgggatg tctggcgtcc gactggaggt caacgttcac atcgtgacgg
     1741 gcgccgtttc ccccgtgcag aacgtggttc ggtcggtcaa cggagtgggc ctggacatca
     1801 acggcgtggt gctggagcag ctcgcggcga gcgaagcggt tttgagcgcc gacgaaaaag
     1861 agctcggagt agccctcgtg gacataggcg gcggaacgac ggacattgcc gtcttctccg
     1921 aaggcagcgt caaatatacg acgatcctgc ctctgggtgg tggttacctg accaacgata
     1981 tagcaatcgg gttgaggacg ccagtcacag aggcggaaaa agtcaagatt cggtacggct
     2041 gtgcctgcac gcccatgatc cctcaggacg agatgatcga ggtcccgagc gtaggcgggc
     2101 gcgaaccgag gagggtctcg cgccaggtct tgggcagcat catcgagcca cggatggaag
     2161 agatcctcaa cctggtatgc cgtgaaatcg taaaatcggg tttcgaagac cggcttgccg
     2221 cgggcatcgt gctgaccggc ggcacggcga tcctcgaggg aatcaccgag cttgcggagc
     2281 atatcttcaa tatgccggtt cggaggggga ttccgatcgg ggtggccggt cctgacgaga
     2341 tcaagagtcc tatttatgca acgggtattg ggttggtttt gtatggagcc aagcagtcat
     2401 caactaacgg gagaaacgag ggcggcttgt tcaggggtgt catgaaaaga ggaagaaaat
     2461 ggttctcgag cattttttag aaggaggtaa agtcgatgtt tgaattgtgt gaggggaagg
     2521 gagacaggtc ggcacgcatc agggtgatcg gaatcggggg cgggggctgc aatgccgtca
     2581 ataccatgat ttcgagcaac ttaactggtg tcgatttcat tgccgcgaac accgacgccc
     2641 aatcgctcaa gacgtcgctc tcaccgacga agatccagct tggcgtgggt atcacgaaag
     2701 gacttggcgc gggcgccgac ccggagacgg gaaggcgcgc cgccgaggag tcggtcgacc
     2761 tgatccgcag cgtccttgaa gaccaggaca tggtgttcat cacggccggc ctcggcggcg
     2821 ggaccggcac gggaggcgct cccattgtcg cgcaggtcgc gaaggagctc ggggcgttga
     2881 cggtggccgt cgtcacgaag ccttttcagt tcgaaggcaa gaagcgggag cggcatgccg
     2941 aagacgggat cgcggaactg cgggggatcg tcgacaccct gatcgtcatc cccaatcagc
     3001 ggctcctcag catgggcggc agaaacatga cgctcctcga ggccttcaag aaagccgacg
     3061 acatcctgta ccaggccgtg aagggcgtat cggacctgat cctcgtgccg ggatacatca
     3121 acctcgactt tgccgacgtg cggacgatca tgtcgggcat ggggctggcg ctcatgggga
     3181 caggcctggc gagaggagaa aacagggccg ttgaggcggc ccagagggcg atctcctcgc
     3241 cgcttctcga ggacaactcc atccaggggg cgcggggggt actgctcaac ataaccggtg
     3301 ggccggacat gagcctcttc gagatcaacg aggcgtcctc cctgatccag gccgaggccc
     3361 acgaggatgc caatatcatc ttcggaacgg tcatcgacga atccatggaa gacgagattc
     3421 gcatcaccgt catcgccacc ggcttcgacg aaagcatggc gaggcaacag gcggccgcga
     3481 atgtttcgca cctgagcagt tacaaacggg aagacctctc gaccccggct ttcctgagga
     3541 agggtgggaa ggcggcgggt gccgagaatc tgaatgtcat caaggtcggt gtgatcgacg
     3601 accttgaagt cgacaatgcc gactttgaag tgccgtcctt cctgagaaag aaggccgact
     3661 gagacgggcc gaagcctgcc ccgtactcga tacggggtcg gagtgcaggg cgggctttgt
     3721 cggtacggca atgcgtctct aaagggctat tcaaaaccga cccagcttgt gatgttatca
     3781 acgggttccc gcgaagtcgt gatcgtattg gcaatgtttc cctcgtccgg gtaaccgagg
     3841 gagatggcaa cgatgaggcg tttcgcttca cttatgccgg cgtgccttct gaccacgtcg
     3901 ggatacatga cgccctggtc gtggatgcag gtacccaggc cgtaatgcaa ggcggcaaga
     3961 cagatcgtct gcatgacggc ccccaaatcc agcagcggcg ctgccgctga aagtgctttg
     4021 tcaaccatga tgatgatcgc ggcaggggcg tcgaagtaac ggaaaccacg ctccatccag
     4081 gcggcccgct tggccttatc ttctctgggg atgttcatga cctgaaagat gtgttttgcc
     4141 aattccacct ggcgggttcg gtacacgctg ttgttcgtcc atccgatcac ttcatggtcc
     4201 gggtgcggct cttcggcggc ccgaaacttc tccgcgcact cctcgcctat cgcacgcaaa
     4261 ggttgccccg aaagcacggc aaactcccag ggctgggtgt tcatggcaga gggcgcgcga
     4321 acggcggtct ccagtatgtc ccggatgact tcccggggga cggcatcttg tctgtagccc
     4381 ctgatactct tacgtttcct gatggcttcg atgatgtcca ctttttcctc ctttctttct
     4441 gtcggttgat tcacccgcct gatgactgac ccaactcggc tctgtacagg aggtattctg
     4501 atgtaactta gcgattatcc aagaaaaatg aaaaaagggg ggtgactgcc gtgtttgtca
     4561 gtccatcctg aaagacaggg cctctcagga tggactgttc agattgctgc cgactttgga
     4621 ctgagtcccg aaagaccttt gactaaaatt cgataacgtc ccggtggcgg aatccccgag
     4681 agtagtcgat caggtatatc ttgaccgggg tcaccttgac gacgaccgag ttgccgggct
     4741 tgacactgtt gaaggcgtta tgccgcgcta tcaccgcctt cggcacttcg gcgtagtcct
     4801 tctcggccag tatctgcgct tttccaaggt attgaattcc tttgatttcc cggtcttctt
     4861 tgggaatggg tccttcttcc tgcattgcta tcgccaggcg agggttggtc tggatgtttc
     4921 tcaccttctg actatctttc cctgtgttga aataaatatc cgcccccgca ttcttgaaga
     4981 aaacagtcgc gatgttcggc tggccgtcag tagtggcagt ggcaatcgtg catgcacggc
     5041 acttctccat gtagttgatg acctgctctt taatatctcc tgccatttcc ctttacctcc
     5101 ttcgctgtct cgtcgtatcg tcgaagctat ccccggtcgt gatacctccc aactcttgaa
     5161 gaaattatca aatgacccct tgaaaccatc ggcttacgaa aacggaagaa gtggtacctc
     5221 ctcaaaaaaa ggactaaacg ctgcacaaga gatacgcttt cgaacttctt ccagggcgga
     5281 ataataccat caattccctc cccatagtca agtcaaaaaa acggctcgtg attttagctt
     5341 gacacaaaac gccttttggg catagtggtt aaccatagca aaaccttgca tggtcttcta
     5401 aggtgcctat ggaccaggcc ctgctgagat ctgaaaaagt tggtctcctg agaaatatct
     5461 cg
//
LOCUS       DAOTMX010000091         5434 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_12652_length_5434_cov_3.669827, whole genome shotgun sequence.
ACCESSION   DAOTMX010000091 DAOTMX010000000
VERSION     DAOTMX010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5434
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12652_length_5434_cov_3.669827"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="P5573_03700"
     CDS             <1..414
                     /locus_tag="P5573_03700"
                     /inference="COORDINATES: protein motif:HMM:NF014614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HRT27203.1"
                     /translation="VQFMKGRKYGEVLCAEKFLPDLTVRQCGLDSFVMKDNPAPVDIE
                     LAGQGLNLAKKVLSSGEYDMVILDEINVALEFKLIALDEVLEIIRNKPREVDLILTGR
                     YAPPEIIEAADMVSEVREIKHHYAKGVKERAGIEF"
     gene            580..1764
                     /locus_tag="P5573_03705"
     CDS             580..1764
                     /locus_tag="P5573_03705"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HRT27204.1"
                     /translation="MRRTPKEELENRITKLQGRMASDGIDGALVMQNADLLYFAGTVH
                     QSFLFVPSSGKPLLLVRKNFERARDESSIDDMVFINDIRQLPVVLRDHGHETSGKLGM
                     ELDVLPVNQYRRFQKLFNPGEVVDISPAVQAIRSIKSPYEVEMMEEAAELVDFMVGVA
                     RSSLKVGMTEVELAGKVEAAARERGHQGYVRMRSFNQEIYWGYLVSGPDSAEPSFIDT
                     TTGGRGVGVAFPSGAGLRTIGPNEPVIFDLVGVVEGYNSDETRTLSVGPLPGRLMEAY
                     RVALEILQSLGDMARPGVAAEKLFLKAEQIADRYGLAEHFMGYGKQKPVFCGHGIGIE
                     LDELPILTRRNAEPLLPGMVFTVEPKFVFPGLGAVGVEDDFVVTETGVRKLTASTYEV
                     EV"
     gene            1748..2398
                     /locus_tag="P5573_03710"
     CDS             1748..2398
                     /locus_tag="P5573_03710"
                     /inference="COORDINATES: protein motif:HMM:NF021561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2284 domain-containing protein"
                     /protein_id="HRT27205.1"
                     /translation="MRWRFDPKSNSGTAGTKKIEETVPAERKKKEPASEKTLEEIILL
                     LIEKGATRGKIIDIADIVVDERVRLKCRIPLCDSFDRNLMCPPRLPSLDEFRQALSRF
                     SGAILVQVSAEFMGPGEAFRHAKTLHELINLGEKEAFEAGFRFAAGLIGGCCRLCDVC
                     VAAEPRGAGEACRFPFKARPSMEAMGIDVIATLESVGWPSGFPVTDRVTWTGLILV"
     gene            2471..2665
                     /locus_tag="P5573_03715"
     CDS             2471..2665
                     /locus_tag="P5573_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27206.1"
                     /translation="MGLLKEAAIPIGRMVFIPKASGMTKDSLEVEANGQYQIIERPDR
                     FEIKNAECCRSILVKVKTKE"
     gene            2685..3653
                     /gene="sppA"
                     /locus_tag="P5573_03720"
     CDS             2685..3653
                     /gene="sppA"
                     /locus_tag="P5573_03720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HRT27207.1"
                     /translation="MNKRLLLVVLVVFFVSGCAFVNIPLYVTPQPLQEKVLEGEGKTK
                     ILLVGVSGLLSETAKDSGSLFREEVPLLSRLKEELQKAEKDRSVGGLIVRINSPGGTV
                     TASDAIHHEITEFKKRTGVRVVACIAGLGTSGAYYVASSADEIVALPTSVTGSIGVIA
                     MRFDVHDLLGKIGVSTETVKSGEKKDLWSPFRPSTPQETEIMQRMIDQLHSRFIDVVA
                     AGRKNVLTRAEVVRLADGRIYTAEQAREAKLVDRIGYLDDALSGIKRSLNVQDAKLIV
                     YHRPDTYRGTIYSDANRLPPVVNLLNVNGDSLSLLPEVRFLYLWNP"
     gene            3654..4643
                     /locus_tag="P5573_03725"
     CDS             3654..4643
                     /locus_tag="P5573_03725"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRT27208.1"
                     /translation="MEDHLLPGVKEVADDLFMITLPMPFRLEHVNVYALIEGDGLSLF
                     DTGGNFPGTFDILEEFLQKVGFSVTRVRRVFITHFHADHCGIAGLIKEKSGASVLMSE
                     TDFGGIRFLDQEGTHLRLLRTFCLEQGLDNGTAEMVAGIFRSFKEITRPFQVDAYLED
                     GSEVTAGDKTVRVVATPGHTRGHCSFFIPEGGILVAGDNVLPHITPNLSIDLLSPAFR
                     PLKSFIGSLEKVSRLPVDTVYPAHGLPFYDLQGRVEEIKAHHRERKALTLRALEGRPK
                     TAVEVSLVIFGSDLPEFDRLLALNETYVHLLELEHEGVIRRYRQGEKYVFTFA"
     gene            4673..5338
                     /locus_tag="P5573_03730"
     CDS             4673..5338
                     /locus_tag="P5573_03730"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRT27209.1"
                     /translation="MELGKAVRERRSIRQFKKEQVPEQVIREILEGARWAPSWGNTQP
                     WEFYVITGRPLETIKKTNRQRVMDGVAPAPDISMPQEWPEYMKARYMGIGKSVLTALN
                     VERDDKESRTRYSADMFYLFDAPCLIVAVQDKGVTSNYRLLDIGLILQTICLLAHDRG
                     LGTCIMACAIGYPDVLRDVAKIPESKTIVMGVTVGYPDLDAPINRFKRTRADVEDFVT
                     WIK"
BASE COUNT         1231 a         1404 c         1658 g         1141 t
ORIGIN      
        1 gttcagttta tgaaagggag aaagtacgga gaggtcctct gcgccgagaa gtttctcccc
       61 gacctcaccg tgcgccagtg cggtctcgat agcttcgtga tgaaagataa tcccgcgccc
      121 gtggacatcg agcttgccgg gcagggattg aatctggcga aaaaagtcct gtcttccggc
      181 gaatacgaca tggtgatcct cgacgaaatc aacgtagcac ttgagttcaa gctcatcgcc
      241 ctcgatgagg tcctggaaat catccgtaac aaaccacgag aagtcgacct gatcctgacg
      301 ggaaggtacg cacccccgga gataatcgag gcggcggata tggttagcga agtccgcgag
      361 atcaagcatc actatgcgaa aggggttaag gagcgggcgg ggatcgaatt ttaagaaaga
      421 ggttgtgagg tccgcgtttc aatcggaaaa acattccgtc ggaaaagcag ctcgcagccg
      481 ccgtccgcag cgaacgaccc tcgtcatcgc ccgtataccc gcctttggtg gaacgacggg
      541 gcggtctttt cccccgacgc aagagagaaa aacggtccga tgcgcaggac tccgaaagaa
      601 gaactcgaaa accgtataac gaaactgcag ggtcggatgg cctccgacgg aatcgacggc
      661 gccctcgtca tgcagaacgc cgacctccta tacttcgcgg ggacggtcca ccagtctttt
      721 ctttttgtac cttcttcggg aaaaccgctg ctcctggtgc gaaagaactt tgaacgggca
      781 agggatgaat cgtccatcga cgatatggta ttcataaatg acatccgcca gttacccgtt
      841 gttttgcgtg atcatgggca tgaaacttcg ggaaaactgg ggatggagtt agatgtcctt
      901 ccggtcaatc agtaccggcg gtttcagaaa cttttcaatc ccggggaggt cgtagacatc
      961 tctcctgcgg tccaggcgat acggagtata aagagtccgt acgaagtcga gatgatggag
     1021 gaggcggccg aactggtgga cttcatggtc ggtgtggcca ggagttccct gaaggtaggg
     1081 atgacggagg tggaactggc aggaaaggtg gaggcggctg caagggaacg ggggcaccag
     1141 gggtatgtcc ggatgaggtc cttcaatcag gaaatctact ggggatacct tgtctccggc
     1201 ccggattccg cggaaccttc tttcatcgat acgactacgg gcggaagggg cgtcggcgtc
     1261 gcctttccct ccggcgccgg cctccgtacc atagggccta acgagccggt gatattcgac
     1321 ctcgtgggcg ttgtagaggg gtacaacagc gatgaaacgc gcactctttc cgtcggtcct
     1381 cttcctggaa ggttgatgga ggcctaccgc gtggcgctgg agatcctcca atccctcgga
     1441 gacatggcca ggccgggtgt agccgccgaa aaacttttcc tcaaggcgga gcagatagca
     1501 gaccgctacg gcctggcgga gcacttcatg ggttacggga aacagaagcc ggtcttttgc
     1561 ggacacggca tcggtattga gttggatgaa ctgccaatcc tgacaaggag aaacgcggag
     1621 ccgctgctcc cggggatggt ttttacggtc gaaccgaagt ttgtttttcc cggcctgggg
     1681 gccgtaggtg tggaagatga tttcgtggtg acggagacgg gcgtgcggaa actcacggcg
     1741 tctacgtatg aggtggaggt ttgatccaaa gtcgaactcc ggaacggcag gaacgaaaaa
     1801 aatagaagag acagtgccgg cagagcgaaa gaagaaagaa ccggcgtcgg aaaagacgct
     1861 ggaagagatc atcctgctgc tgatagagaa aggcgcgacg cggggaaaga taatcgacat
     1921 agcggacatc gttgtggacg aacgggtgcg tctcaagtgc cggatacctc tctgcgacag
     1981 tttcgaccgt aatctcatgt gtcccccgcg actgccgtcc ttggatgaat tcaggcaggc
     2041 cctgagcagg ttttcgggtg ccatcttggt ccaggtttct gcggaattca tgggccccgg
     2101 cgaggccttt cggcatgcga agacactgca tgaactgata aatctgggag agaaggaggc
     2161 tttcgaggcg ggattccgtt ttgccgccgg tttgatcggc gggtgttgcc ggctttgtga
     2221 tgtatgcgtg gcggccgagc ctcggggtgc cggcgaggcc tgtcggtttc cttttaaggc
     2281 ccgtccttcc atggaagcga tggggataga tgttatcgct acgctggagt cggtaggatg
     2341 gccgtcggga tttccggtga ccgaccgggt cacctggacg gggctcatcc tcgtttgaag
     2401 taacggatgc gcggtgaaag gtgaaagact tttcccccgc ggtttttgat tcatcataag
     2461 gaggtttgtc atgggtttgc tcaaagaagc tgcgattccc atcgggcgca tggtgttcat
     2521 tcccaaggcg agtggtatga cgaaagacag cctggaggtc gaagcgaacg gacagtacca
     2581 gattatcgag aggcctgacc gtttcgagat caaaaacgcc gaatgctgcc ggagtatcct
     2641 agtaaaagtt aaaacaaagg aatgaacgtc tcgggagttc taccatgaac aagcggctac
     2701 tgcttgtagt gctggtggtt ttttttgtct cggggtgcgc cttcgtcaac atccccctct
     2761 acgttacacc gcaacccttg caggaaaaag tccttgaagg tgagggaaag accaagatcc
     2821 tactggtcgg cgtatcgggg ttactgtccg aaacggcaaa ggatagtggg tctctcttca
     2881 gagaagaggt tcccctgctc tcacggttga aagaggagct ccaaaaagcc gagaaggaca
     2941 gaagcgtagg cgggttgatc gttaggatca attcccccgg gggaacggtg acggcatcgg
     3001 acgccatcca ccatgaaatc acagagttca agaagcggac gggcgttcga gttgtcgcct
     3061 gcatcgccgg cttggggaca tcgggggcct actatgtcgc ttcctcggcc gatgaaatcg
     3121 tggcccttcc cacctctgtc acgggtagca tcggggtgat agcgatgagg ttcgacgtcc
     3181 acgacctcct cggcaagatc ggcgtcagca cggaaacggt aaagtcgggc gagaagaaag
     3241 atttatggtc ccccttccgt cccagcacac cgcaggagac cgagatcatg caacggatga
     3301 tcgatcagct ccacagccgc tttatcgatg tcgtcgcggc gggaaggaag aatgtgctga
     3361 cccgggcgga ggtcgtgaga ctcgccgacg gccggattta cacggccgaa caggcccggg
     3421 aagcgaagct cgtggaccgc atcggatacc tggacgatgc cctttccgga atcaagcggt
     3481 cgttgaatgt ccaagacgcc aagctgattg tgtaccaccg gccggatacg tacaggggga
     3541 cgatttattc ggacgccaac cgattgcccc cggtggtcaa cctactgaac gtcaacggag
     3601 atagtctgtc tcttctcccg gaagtaagat ttctctacct gtggaatccc tgaatggaag
     3661 accatctttt accaggcgtc aaagaggttg ccgacgacct cttcatgatt accctgccga
     3721 tgccctttcg cctcgaacac gtgaacgtct atgcccttat tgagggcgac ggtcttagcc
     3781 tattcgacac ggggggaaat ttcccgggga cgttcgacat cctcgaagag tttttgcaga
     3841 aagtaggttt ttcggtcacc cgggtgcggc gtgtattcat cacccacttt catgccgacc
     3901 actgcggaat cgccggcctg atcaaagaga agtccggggc ctccgttttg atgtcggaga
     3961 cggattttgg gggcattcgc ttcctcgatc aggaagggac tcacctccgg ctgcttcgga
     4021 ctttctgcct cgagcagggt ctagacaacg ggacggccga gatggtagcc gggattttta
     4081 ggtcctttaa ggagattacc cggccctttc aggtcgatgc gtacctggaa gacggctcag
     4141 aagtaaccgc cggggataaa acggtcagag tggttgcgac gccggggcac acgagggggc
     4201 attgttcgtt tttcattccc gagggcggca tcctggtcgc gggcgataac gtcctgcccc
     4261 acataacacc taacttgagc atcgaccttc tttcaccggc gttccggcct ttgaagagtt
     4321 tcatcggatc gctggaaaag gtcagccgcc tcccggtcga cacggtatac cccgcacacg
     4381 gcctgccctt ttatgatctg caagggcggg tggaagaaat caaggcacac caccgggaga
     4441 gaaaggcctt gacgctccgt gcactcgagg gccgcccgaa aacggccgtg gaggtctccc
     4501 tggtaatttt tgggagtgac cttcccgaat tcgacagact tctcgccctc aatgagacat
     4561 acgtacatct cctggagctg gagcatgaag gcgtcatcag gcggtatcga caaggagaaa
     4621 agtatgtttt cacctttgcg tgaaccattc ttatcgatgg aggggatttg acatggagct
     4681 cggaaaagca gtcagagaaa gaaggagcat caggcagttc aaaaaggaac aggttcccga
     4741 acaggtcatc cgggaaatac tcgaaggggc gagatgggcg ccgtcatggg gtaataccca
     4801 gccgtgggag ttctacgtca tcaccggccg gccgttggaa accatcaaga aaacaaaccg
     4861 gcagagggtg atggacggag ttgccccggc gccggacatc agtatgcctc aggaatggcc
     4921 ggagtatatg aaggcgaggt acatgggcat cggaaagagc gtcctcaccg ctttgaatgt
     4981 cgagcgcgac gacaaggaat cccgcactcg ctacagcgcc gacatgttct accttttcga
     5041 tgccccctgt ctgatcgtgg ccgttcagga taagggcgtt acgagcaact accgactcct
     5101 cgacatcggg ctcatcctgc agacgatctg tctcctcgcc cacgacaggg gcctcgggac
     5161 ttgcatcatg gcctgtgcca tcggctaccc tgacgtcctc agggatgtag cgaagattcc
     5221 agagtcgaag accatcgtga tgggtgtcac cgtcggttat cccgacctgg atgccccgat
     5281 aaaccggttc aaaagaaccc gggctgacgt cgaggatttc gtgacctgga taaagtaggt
     5341 gacgtccttc caggatgcaa aagtcggggg tagggccctc ttactgaatt tatttcctgc
     5401 ggtccttgcg cttgtgccgg tggcttctct cggc
//
LOCUS       DAOTMX010000092         5409 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_12716_length_5409_cov_2.843855, whole genome shotgun sequence.
ACCESSION   DAOTMX010000092 DAOTMX010000000
VERSION     DAOTMX010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5409
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12716_length_5409_cov_2.843855"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1075
                     /locus_tag="P5573_03735"
     CDS             <1..1075
                     /locus_tag="P5573_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27210.1"
                     /translation="NFYDTGDELYAVMTQMEKKLGVRSFFVMDENFLLHRRRALRLLK
                     LMEENRKSWSLYVFSSARVLRSYTMEQLVGLGISWVWMGLEGENSRYQKLHGVDTLSL
                     VRELQSHGIRVLGSSIIGTEDHTPENIADVIAYAVRHDTDFHQFMLYTPVPGTPLYEE
                     HRSKGTLYSPEEFSLADAHGQYRFNYRHEHIHDGREEQMLLDAFRTDFEVNGPSLYRL
                     IRTIMAGWKRYKDHPDKRIRNRFRREVRPLRGGYAGALWAMRRYYAGNDRVVGKVDEL
                     LEEIYEEFGWKTRFLAPVIGRYLYGAAKKEEARLAQGWTYEPQFFCQKNTMALALEGL
                     RRAGRTRVQELRWVTCKHLSPLP"
     gene            1274..2146
                     /locus_tag="P5573_03740"
     CDS             1274..2146
                     /locus_tag="P5573_03740"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRT27211.1"
                     /translation="MKAIDVQVQTFTKEARKFNPDPEGIELMARKVFKRESVFIAEEE
                     LIAGLRKAQVKAVLMSPMGWARAGRDMAQIREMHDYMGHLKKAYPDVVLGCWGDIDPR
                     LGYQGARELERCIKDVGMMGIYTGAILTGIPVNDKLYWPLYGVCAEAGAAVKLSVGMT
                     AIGQGTPGGGGIHLSRENPIPYVDEVAIRFPELTIIAAHCAWPFQNEMIAVMVHKANV
                     HNELHGWSPKYFPPEMKREINGRLQDRIMFGTDHPWFTYERIFNDWETEGYKPEVLEK
                     VYHKNARRILRLEV"
     gene            complement(2157..3536)
                     /locus_tag="P5573_03745"
     CDS             complement(2157..3536)
                     /locus_tag="P5573_03745"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HRT27212.1"
                     /translation="MVTVQATCRPTVYFETAARVVKTLKEAGHEAYFVGGSVRDILRG
                     LEPVEFDIVTSATPKEVRALFDHTVAVGEKFGVILVIEEGLSYEVATYRIDRAYEDGR
                     RPLRVEFAALAEEDVRRRDFTVNGLLMDPGTCLVFDYVGGVEDLKKRIIRTIGDPEER
                     FAEDHLRMLRAVRLAANLGFEVEPATFAAIKRNAKAIRRISAERIRNEITRTITRGGA
                     RRGMELLADSGLLAEVLPEVEALRGVTQPERFHPDGDVWEHTLRMLDMLPGRGKREAD
                     PRLAWGVLLHDVGKPLTRSEDARGIHFYGHSKKGEEIAEAVMQRLRFPRSDVEAVVAM
                     IRHHMLFINVMDMRPNRLKRFLRMPDFELHLELHRLDSLASHGNLENYEFCRKKLAEF
                     ASEDLRPPPLITGKDLIAMGFEPGPLFKEIKRCVEDAQLDGEISTPEEARRLVLEKWG
                     GRRKEEPRP"
     gene            complement(3926..5173)
                     /locus_tag="P5573_03750"
     CDS             complement(3926..5173)
                     /locus_tag="P5573_03750"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HRT27213.1"
                     /translation="MEKINPFDVVKQQVSKCAEILHLPPDVAAMLKTPMRELHVSLPV
                     RMDDGSVRVFPAFRVQYNDARGITKGGIRFHPEETIDTVRALAAWMTWKCALLDLPLG
                     GAKGGIVCNPKEMSAGELERLSRSYIRSVFQFIGPEKDVPAPDVYTTPQIMAWMMDEY
                     SVIAGKPQFGVITGKPLPLGGSPGRGDATARGGMFTIREAAKALGIDLSKATVAVHGY
                     GNAGYYAAQLSKELFGSKIVAVCDSKGGVHSKNGIDPEEAYTCKCRTSSVSNLPGFER
                     ISAEELLELDVDILIPAAIENVITDKNAPNIKAKIVAELANGPTTPEADDILHRNGVH
                     VIPDFLCNAGGVTVSYFEMVQNFYLYFWDEMEVRERLDKKMTSAYHSVLSTSREYNIN
                     MRQAAYVRAVERVVEAMKLRGWV"
BASE COUNT         1121 a         1550 c         1547 g         1191 t
ORIGIN      
        1 caatttctat gacacgggag acgagcttta cgcagtcatg acccagatgg agaagaaact
       61 cggcgtccgg tcattcttcg tcatggacga gaatttcctc ctccaccgga ggagggcact
      121 ccggctcctg aaactcatgg aagaaaaccg caagagctgg agcctctatg tcttcagctc
      181 cgccagggtt ctccgctctt acacgatgga gcagctcgtg gggctgggta tctcctgggt
      241 ttggatgggt ctggaggggg agaacagccg gtaccagaaa ttacacggcg tcgacacgct
      301 ctcgctagtc agggagttgc aatctcacgg catccgcgtc ctcgggtcgt cgatcatcgg
      361 cacggaggac cacacgcccg agaacatcgc tgatgtgatt gcctacgccg tcagacacga
      421 cacggacttc caccagttca tgctctacac gcccgtgccg ggcacacccc tttacgaaga
      481 acaccgcagt aagggaaccc tgtattcgcc ggaggagttc tcccttgccg acgcccacgg
      541 tcagtaccgc ttcaattacc gccacgaaca catccacgac ggtcgagaag agcagatgct
      601 cctcgacgcc ttccgcaccg acttcgaggt caacggcccc agcctctacc gtcttatccg
      661 gacaatcatg gcaggctgga agaggtacaa ggaccatccg gacaagagaa tccgcaaccg
      721 cttcaggaga gaggtgcggc ctctgcgggg tgggtatgcg ggtgccctct gggcgatgag
      781 aagatactat gccggcaacg acagggtcgt gggtaaggtc gacgaactct tggaagagat
      841 atacgaagag ttcggctgga agacccgttt cctcgccccc gtgatcgggc ggtacctgta
      901 cggcgccgcg aaaaaagagg aggcgcgact agcccaaggt tggacttacg agccgcagtt
      961 cttctgccag aagaacacca tggcccttgc cctcgagggt ctccgcagag ccggcaggac
     1021 ccgcgtgcag gaactccgct gggtaacctg caaacacctg tcccccctcc cttaaccagt
     1081 tctaatcact acgagaaaaa tggtctgccc tgagtattgc gatgtcgggt cagctcgggg
     1141 cctgacggca tccccgcttt ctctgaaaat cgcggcctct gaaaatcgcg gccttgacag
     1201 gaaccatcat cccttctatt gtttttccgt ccttatttgt cggtcaaagg aaaaatagaa
     1261 ggaggatgag gtcgtgaagg ctatcgatgt tcaggtccag acgttcacga aagaggccag
     1321 gaagttcaac ccggacccgg aagggatcga gcttatggcg cgcaaggtct tcaaacggga
     1381 atccgttttt atcgccgagg aggaactgat cgccggcctg agaaaggcgc aggtcaaggc
     1441 cgtcctgatg agtcccatgg gttgggcgag ggccggccgg gacatggctc agatccggga
     1501 gatgcacgac tacatgggtc acctcaagaa ggcctacccc gatgtagtcc ttggttgctg
     1561 gggcgacatc gaccccaggt tgggctacca gggggcgcgg gagctggagc ggtgcatcaa
     1621 ggacgtcggt atgatgggga tctacacggg cgccatcctg acgggcatac ccgtcaacga
     1681 caaactttac tggccgttgt atggggtctg cgccgaagcg ggcgcggcag tcaagctttc
     1741 cgtgggcatg accgccatcg ggcaggggac cccgggagga ggcgggattc acctgagccg
     1801 tgaaaacccg atcccctacg tggacgaggt ggcaatccgg tttcccgaac tgactatcat
     1861 tgccgcccat tgcgcctggc ccttccagaa cgagatgatc gccgtgatgg tccacaaggc
     1921 gaacgtccac aacgagctcc acggctggtc gccgaagtat tttccgccgg agatgaagcg
     1981 ggagataaac gggcgcctcc aagacaggat catgttcggc accgaccatc cctggttcac
     2041 gtatgagcgt attttcaacg actgggagac cgagggctac aagccggagg tcctcgagaa
     2101 ggtctaccac aagaacgccc ggagaatatt gaggttggag gtatgaagcc cggtcgttac
     2161 gggcgcggtt cctcttttct cctcccgccc catttctcca gtacgaggcg ccgggcctct
     2221 tccggtgtcg agatctcccc gtccagctgc gcgtcctcga cgcacctctt gatttccttg
     2281 aatagaggcc cgggctcgaa ccccatggct atcaggtcct taccggtgat gaggggtggg
     2341 gggcgtaagt cctcgctcgc gaattcggcg agtttcttcc tgcagaactc gtaattttcg
     2401 aggttgccgt ggctcgccag gctgtcgagg cgatggagct ccagatgtag ttcaaaatcc
     2461 ggcatcctca gaaacctttt gaggcggttc ggcctcatgt ccatcacgtt gatgaagagc
     2521 atgtggtggc gtatcatggc gaccacagcc tcgacgtccg agcggggaaa tcggaggcgc
     2581 tgcatgaccg cctcggcgat ttcttcgccc ttttttgagt ggccgtagaa atggattccc
     2641 cgtgcgtctt cggatcgcgt gaggggtttt ccgacgtcat ggaggaggac gccccaggca
     2701 aggcgtgggt cagcctccct ttttccccgg cccgggagca tgtcgagcat gcgtagtgtg
     2761 tgttcccaga cgtcaccatc ggggtggaag cgctccggct gggttacacc ccgcagggcc
     2821 tcgacctcgg gcaggacttc agcgagaagg cctgagtcgg cgaggagctc catcccccgc
     2881 cgtgccccac cgcgggtgat tgtccgtgtg atctcgttcc ggatgcgctc ggcgcttatg
     2941 cgccggatgg cctttgcgtt tctctttatg gccgcgaacg ttgcgggttc gacctcgaaa
     3001 ccgaggtttg ccgcgagccg gaccgcccgg agcatcctca ggtggtcctc ggcgaatcgc
     3061 tcttccgggt cgccgattgt gcggatgatt cgtttcttaa ggtcctcgac cccgcccacg
     3121 taatcgaaga cgagacaggt gccggggtcc atgaggaggc cgttgacggt aaaatcgcgg
     3181 cgcctgacgt cttcttcggc aagggcggcg aattcgacgc gcaaggggcg ccgcccgtct
     3241 tcgtaagccc tgtcgatgcg gtatgtggcg acctcgtaac taaggccctc ctcgataacc
     3301 aggatgacgc cgaatttttc gcccaccgcg accgtgtggt cgaaaagggc gcggacttcc
     3361 ttcggcgtcg ccgacgtgac gatgtcgaat tccaccggtt cgagaccccg gaggatgtcc
     3421 cgcacgcttc cgccgacgaa gtatgcctcg tgccccgcct ctttaagcgt ttttacgacc
     3481 cgggccgcgg tttcgaaata aacggttggt cggcaagtgg cctgtacagt gaccataggt
     3541 tgtccatccg gcgcatgatc ataacccctt cgccggtgag tcagtgagat ggggggcgct
     3601 gcatcgcaaa tagagataga gcccgttcaa tagttcaagt ttaggacaag gctttttatc
     3661 gtccttgaga tgtccatata ctatgtcctg agtgaggacg atatcatgtt caacgacgtg
     3721 gatggtaaca ttttcgataa tgtattgaaa gtattacgag atggcttgat gtcctgggtg
     3781 acgaaatatg gaagaaatcc cccgagccac cttgacaagt ccggccaccg accacgttga
     3841 tcgtctggtg gtcgatggcc ggcaaaatag ctatgggtaa tcgggcaaag atgaccctca
     3901 agtaccccgc tgagaggtca atacactaaa cccagccccg gagcttcatt gcctctacca
     3961 cccgctcaac ggccctaacg taagccgcct gtcgcatgtt tatattgtac tctcgagagg
     4021 tactcaacac ggaatgatag gccgaggtca tcttcttgtc cagtcgctct cgaacctcca
     4081 tttcatccca gaagtacagg tagaagttct gaaccatctc gaagtaagag acagtcacac
     4141 caccggcgtt gcacagaaag tcgggaatca cgtggacccc gtttctatgt aaaatatcgt
     4201 cggcttccgg cgtagttggg ccgttagcca gctcggctac aatcttggct ttgatgttcg
     4261 gcgcgttctt gtctgttatc acgttttcta tggcggccgg aatgagtata tctacgtcga
     4321 gctcaagaag ctcctctgcg gatatgcgtt cgaatccggg caggttggac accgaagaag
     4381 tacggcattt acacgtatag gcttcttccg ggtcgatacc gtttttgctg tgtacgcctc
     4441 ctttgctgtc acaaacggct acaatcttcg acccgaaaag ctctttgctc aactgagccg
     4501 cgtaatatcc ggcgtttccg taaccatgca cggcgacggt ggccttgcta aggtcgatgc
     4561 cgagtgcctt tgcagcttcg cggatggtaa acatcccacc gcgagctgta gcatcacccc
     4621 ttcccggaga accgccgagg ggcagcggtt tacccgtgat gacaccgaat tgcggtttcc
     4681 ccgcgatgac cgaatactcg tccatcatcc aggccatgat ctgcggggtg gtataaacgt
     4741 caggggccgg tacgtctttc tcgggtccga tgaactgaaa tacactacgt atgtatgacc
     4801 ggcttaggcg ttccagttcg cccgcagaca tctccttcgg attgcagact atacctcctt
     4861 tggcgccacc aaggggtaag tccaacaagg cgcacttcca tgtcatccaa gcagccagtg
     4921 ctcgaacggt atcgatcgtt tcctcgggat ggaacctgat acctcccttt gtgatgcccc
     4981 tggcgtcgtt gtattgaacc ctgaacgccg ggaataccct gacagaaccg tcatccatcc
     5041 gcaccggtag cgatacatga agctctctca tgggagtttt gagcattgcc gctacgtccg
     5101 gcggtaggtg taggatctct gcgcatttgc tgacctgttg tttcacgaca tcaaacggat
     5161 ttatcttttc cacgttcttc tatcctcctt ttcgacgtat ctcatgatta cgacagtctg
     5221 tctcattctt tcccggaagg cggcgccttc cttgaaaaag gaaaccgtta ataacacagc
     5281 tgacaattta gcgcaacacc ccattttttc tatggtttgc ctcagcttcc gtcgttgttt
     5341 ttcccttgtg gggaaaagtc ggggccgccg gagtggttga ttcttgaccc aaagtctccg
     5401 ttccatgaa
//
LOCUS       DAOTMX010000093         5404 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_12729_length_5404_cov_4.536362, whole genome shotgun sequence.
ACCESSION   DAOTMX010000093 DAOTMX010000000
VERSION     DAOTMX010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5404
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12729_length_5404_cov_4.536362"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="P5573_03755"
     CDS             <1..659
                     /locus_tag="P5573_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HRT27214.1"
                     /translation="YAGSGGFLRRYTADEVAVQESVNTRKGVERCIRFAFDFCRRRNR
                     RKKLTLCGKTNVLTYAWDLWERAFHEVGREYPDIRKDYAHVDAICMWMVKNPEFFDVI
                     VTDNMFGDIITDLGAMIQGGLGIAAGGNINPEGVSMFEPIGGSAPKYTGLNKINPLAA
                     ICAVQMMLEHLREEKAADILLEAVKKAVKNDIKSLDAGKMGYGTREVGDLIVKYINQG
                     "
     gene            747..1352
                     /locus_tag="P5573_03760"
     CDS             747..1352
                     /locus_tag="P5573_03760"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HRT27215.1"
                     /translation="MLLAINSDNPQTRLIRKTVDILRDGGIIVYPTDTVYGLGCDLFN
                     KRGIEKIYEIKRRSMKQPFSFICADLKDISNYAIVSNYAYKTMRRMLPGPYTFILEAT
                     RLVPKILLTKRKTVGIRVPDNEICLSIVRELGHPIISTSVTTGDEILSDPAEIEKKYG
                     RAVDLVIDGGILATEKSSVVSLVNDIPEIIRVGKGDVSLFQ"
     gene            1370..3163
                     /locus_tag="P5573_03765"
     CDS             1370..3163
                     /locus_tag="P5573_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HRT27216.1"
                     /translation="MKKIMLINAIDREEQRMAIVENGKLVEFNLQMAEHEPITGNIYK
                     GIVQKVERGLQAAFVDYGGKKNGFLPLHDVSPDYYKSEGADGEGHKRHGLKSGQELLV
                     QVVREEKDRKGAMLTTYISLPGRYLVYLPNREGAGVSRKIEDEAERKKLKEFVDQVLK
                     KDEKAGFIVRTAGQSRKKQELLRDYQHLHRLWKEITKKAAQVSAPALIYQESDFGVRS
                     LRDYFTPDISEILIDDPDTFKKVRDYCKIVQPRNVKMIKLYKEHTPLFEKYQLEKQTD
                     EIYQERVTLKSGGSIVITPTEAMITIDVNSGRGSNRKDVEETAFRTNLEAVEVIARQL
                     RLRDLGGLIVVDFIDMRERKHISEVEKAFKKALSLDRARIQMSRISKFGIMELSRQKK
                     QSAIQDISYTTCPQCKGSGLRPSLEYIALGIYRKIKAEVVRGDTASIKVTLPWEVSDY
                     LLNQKRAELTNIEKTYETNIHVSGSADMHWGESKFEKVRKPEAEKELQPEVVQPEEHT
                     ASPPEEETVKEAVPEGEEGQAVAAEAEEPTKKKPARRRPRRRRRKTAEQTAGQPSESA
                     AAPEGKADEEKEVVEEKKKDKSILEKLFFSL"
     gene            complement(3275..3350)
                     /locus_tag="P5573_03770"
     tRNA            complement(3275..3350)
                     /locus_tag="P5573_03770"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3315..3317),aa:Lys,seq:ctt)
     gene            complement(3381..3457)
                     /locus_tag="P5573_03775"
     tRNA            complement(3381..3457)
                     /locus_tag="P5573_03775"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3421..3423),aa:Arg,seq:tcg)
     gene            complement(3494..3567)
                     /locus_tag="P5573_03780"
     tRNA            complement(3494..3567)
                     /locus_tag="P5573_03780"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3531..3533),aa:His,seq:gtg)
     gene            complement(3580..3656)
                     /locus_tag="P5573_03785"
     tRNA            complement(3580..3656)
                     /locus_tag="P5573_03785"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3620..3622),aa:Arg,seq:tct)
     gene            complement(3772..>5404)
                     /locus_tag="P5573_03790"
     CDS             complement(3772..>5404)
                     /locus_tag="P5573_03790"
                     /inference="COORDINATES: protein motif:HMM:NF040132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27217.1"
                     /translation="KKIIVLFVVSVVLAFLPSGVTPAEDDSGAAVTLQVSPEVMDLGV
                     LAPAGEVKGIYFMHCPSSKTLAWSTDGPEGWIAVGDGKRLASRMTDSLGRLEVGLRFV
                     GGETADEMGRYLCRITLKSEGETLTLEKALPLGQHSEKINVHYEGGSRTLFLKFETAL
                     SEPGPKLSVEPFGLDFGTIEGSRTTLKKLRVRNVGKGTLTWKTGIGGASEGRTGKGLY
                     VSFLNENRREGEAYAVPGELETVLQLNGKWFTDRGYPAVKGEEGTMTFFFSGTGIAIV
                     VHPEKGEGTLKAFVDDLAPVEIDGDPEEGRTAAVVAENLSEGRHILKVMSERAFVMVE
                     GVRIFGGEVLTGVPGWLRVFPSTGTTTSETDYVTVSVDTENLKAGLYGDVVVVRSNGG
                     DTAVDVSLRVTRNIATKFIDVYRYVKGNDYLYTGHPEAEDADFLKGYRRQGLAFRLFW
                     EDTPGTKKFYRWYNKAKGDHYYTTDPDGDGKSRQGYVLEGSIGNIGTSKILGTRELYR
                     WQNPSTGCHFYTTDLRGEGMAKKGYRFDGIAGYVR"
BASE COUNT         1399 a         1489 c         1420 g         1096 t
ORIGIN      
        1 tttacgcagg aagcggcggt ttcctgaggc gttacacggc cgatgaagtc gccgtccagg
       61 aatccgtgaa cacccggaag ggcgtcgaga gatgcatccg ctttgccttc gatttttgcc
      121 gcaggcggaa ccggaggaag aagctcaccc tctgcggtaa gaccaacgtc cttacatacg
      181 cctgggacct ctgggagcgc gccttccacg aagtgggccg ggaatacccg gacatccgga
      241 aagattacgc tcatgtggac gccatctgta tgtggatggt gaagaacccg gaatttttcg
      301 acgtcatagt cacggacaac atgttcggtg acatcatcac agacctaggc gccatgatcc
      361 agggcggtct ggggatcgcc gccggcggca acataaaccc cgagggtgta tcgatgttcg
      421 aacccatcgg cgggtctgca ccgaaataca ccggtttgaa caagatcaat ccactggcgg
      481 cgatctgcgc cgtccagatg atgctcgaac atcttcgaga agaaaaggcg gcggacatcc
      541 ttctcgaggc cgtcaaaaag gctgtcaaga acgacatcaa gagcctcgac gcggggaaga
      601 tgggctacgg cacgcgcgaa gtgggcgacc tgatagttaa gtacataaac cagggttaga
      661 ttcttttatg ctcagtacaa tatcccgttc ttgctaaacc cacctgattc tgataaatta
      721 aatcagtttt caggaagcag gttatcatgc tgctcgcaat aaacagcgat aacccccaga
      781 cgaggctcat cagaaagacc gtagatatcc tgagagacgg cggcatcatc gtctatccga
      841 cggatacggt ctacggcctg ggatgtgacc tcttcaacaa aagggggatc gagaagattt
      901 acgaaatcaa gcggcggagt atgaagcagc ctttcagctt catctgcgcg gacctcaagg
      961 atatcagcaa ttacgccata gtttcgaact atgcctataa gaccatgcgg cggatgcttc
     1021 cgggaccgta taccttcatc ctggaggcaa cgcgtctcgt ccctaaaatc ctcctgacaa
     1081 aaagaaaaac cgtgggcatc agggttcctg acaacgaaat atgtttgtcc atcgtcaggg
     1141 agctggggca tcccatcatc agcaccagtg tgacaacggg ggatgaaatc ctgagcgacc
     1201 cggcggaaat agaaaaaaaa tacggccgcg ccgtggatct cgtgatcgac ggcggcatcc
     1261 tcgccacaga aaagtcgagc gtggtgagtc tcgtgaatga tattcctgag atcatcaggg
     1321 tcggcaaagg ggatgtaagc cttttccagt aaatagacaa ggaatcgtta tgaagaaaat
     1381 catgttgatc aacgcgatcg accgggaaga acagcgtatg gcgatcgtcg aaaacggcaa
     1441 gctggtcgaa ttcaacctcc agatggccga gcacgaaccc ataaccggca acatctacaa
     1501 aggcattgtc cagaaagtgg aacgggggct tcaggcggcc ttcgtggatt acggcgggaa
     1561 gaagaacggc tttctccctc tgcacgatgt cagtcctgac tattacaaga gcgaaggggc
     1621 ggatggtgag ggccacaaga ggcatggcct caaatctggc caggagttgc tcgtccaggt
     1681 agtgcgcgaa gaaaaagacc gcaaaggggc gatgcttacg acctacatct ccctccccgg
     1741 ccggtacctg gtgtacctgc cgaaccgtga aggagccggt gtctcaagga aaatcgaaga
     1801 cgaggccgag cgcaaaaagc tcaaggaatt cgtagaccaa gtcctcaaaa aggacgaaaa
     1861 ggcgggcttt atcgtccgca cggccggtca gagcaggaag aaacaagaac tcctgcggga
     1921 ctaccaacac ctccaccgcc tctggaagga aataacgaag aaagccgccc aggtctcggc
     1981 cccggcgctc atctaccagg aaagtgattt cggtgtaagg tcgcttcggg attacttcac
     2041 gccggacata agcgagatac tgatcgatga ccccgacaca ttcaagaagg tgcgcgatta
     2101 ctgcaagatc gtccagcccc ggaacgtcaa gatgatcaag ctctacaagg agcacacacc
     2161 ccttttcgag aaataccagc ttgaaaaaca gaccgacgaa atataccagg aacgggtgac
     2221 cctgaaatcg ggcgggtcga tagtgatcac tcccacagaa gccatgataa cgatcgacgt
     2281 aaattcggga aggggttcaa acaggaaaga cgtcgaagag acggccttca ggacgaacct
     2341 agaagcggtc gaagtcatcg cgcgacaact caggctgcgc gacctcggcg gtctcatcgt
     2401 ggtcgatttc atcgacatga gagaacgaaa acacatctcc gaggtagaga aggccttcaa
     2461 gaaggccctc agcctcgacc gggcaaggat ccaaatgtcc cgcatttcaa agttcggcat
     2521 catggagctt tcccgccaga agaaacagtc ggcgatccag gatatcagct acaccacctg
     2581 cccgcaatgc aaaggatccg gcctcaggcc gtcgctggaa tacatcgctc tcggcatcta
     2641 ccgtaagatc aaagccgagg tcgtacgagg ggatacggcg tcgatcaaag tcaccctgcc
     2701 ctgggaagtc tccgattacc tcctcaacca aaagcgcgcc gaattgacaa acatcgagaa
     2761 aacttacgaa acaaacatcc acgtttcggg aagtgccgac atgcactggg gagagtcgaa
     2821 gttcgaaaag gtgcggaaac cggaagcgga gaaggagctt cagcctgagg tggttcaacc
     2881 ggaagagcat acggcctcac cccctgagga agaaaccgta aaagaagccg ttccggaagg
     2941 ggaagagggt caggcggtgg cggctgaggc ggaggaaccg actaagaaga aacccgcgcg
     3001 aagacggccg cgccggagaa gacgaaaaac agccgagcaa acggcggggc aaccgtccga
     3061 atcggcagca gctcctgagg gaaaggcaga cgaagaaaaa gaagtggtgg aggaaaagaa
     3121 gaaggataag tcgatcctcg agaaactttt cttcagcttg tgaacaattt ctctttgaaa
     3181 gtcagttaaa aataggcctt cttagttatt tcatgaaggc tactggtatc cctgcccttc
     3241 cagtcctaat gaccggtcgg gcagtttaaa ttactggtgg gccgccgggg actcgaaccc
     3301 cgcgccaatt gattaagagt caactgctct accgactgag ctagcggccc aggctgatac
     3361 tgttcctcag cttcgatggc tggtacgccc ggtaggattc gaacctacga ccaacagatt
     3421 cgaagtctgc cactctatcc agctgagcta cgggcgctta tatcgttcag ggcatgtcgc
     3481 ccactttatc cggggtgagt gaagggactc gaaccctcaa ccttcggggc cacaacccga
     3541 cactctgacc agttgagcta cacccaccac gattttttat ggtacgcctg cagggattcg
     3601 aacccgggac ctacggatta gaagtccgtt gctctatcca gctgagctac aggcgcacaa
     3661 tctctacaga cgctactcaa taaacaaaag gagcgtttta ataaactctt tctaattatt
     3721 tgtcaagcct gaaagcaaaa tcagccgggg tataacgcga cactgcagca atcatcttac
     3781 gtacccggcg ataccgtcga atcggtaccc tttttttgcc atcccctcac ccctcaaatc
     3841 ggtcgtgtag aaatgacagc ctgtcgaggg gttttgccag cggtaaagtt cccgcgtccc
     3901 gagaatctta gaagtaccta tgtttccgat agacccttca aggacataac cctgacggct
     3961 ttttccgtcc ccgtccgggt cggtcgtgta gtagtgatcc ccttttgcct tattatacca
     4021 tcggtagaac ttcttcgtac ccggagtatc ttcccagaac agtctaaagg ctaacccttg
     4081 tctcctgtat cccttaagga aatcggcgtc ttccgcttcc gggtgccctg tgtacagata
     4141 atcgtttcct ttgacatagc ggtaaacatc gatgaatttt gtggcgatat tccgtgtcac
     4201 cctgagcgaa acatccacgg cggtgtcacc gccgttcgac ctgacgacca ccacgtcgcc
     4261 gtaaagaccc gctttcaggt tttccgtgtc gacggagaca gtgacgtaat cggtctcgct
     4321 cgtcgtcgtc ccggtggacg gaaagaccct aagccatcca gggacacccg tgaggacctc
     4381 accgccgaaa attcttacac cttcgaccat gacgaacgcc ctctcgctca tgaccttgag
     4441 aatatggcga ccctcagaga ggttttctgc aacaaccgct gccgtccttc cttcttccgg
     4501 gtctccatct atctcaacag gagcgaggtc atcgacaaaa gccttcaaag tgccttctcc
     4561 cttttcagga tgaacgacga tcgcgattcc tgtaccggaa aaaaagaagg tcatcgtccc
     4621 ttcttccccc ttgacggcgg gatatcccct atctgtgaac catttaccgt tcaattggag
     4681 aacggtttcc agctccccag ggacggcata ggcctcgcct tctcgtctgt tttcattcag
     4741 gaacgacacg taaagtcctt tcccggtgcg gccttccgac gcaccaccta tccccgtctt
     4801 ccaggtgagc gttcccttgc cgacattccg gacgcgcaat tttttcaggg ttgtacggct
     4861 cccctctatc gtcccaaagt cgaggccgaa aggttctaca gaaagtttcg ggcccggctc
     4921 actgagggct gtttcgaact tgagaaaaag cgtccttgaa ccgccctcat aatggacgtt
     4981 gattttttcg ctgtgctgtc ctaagggtag tgccttctca agggtaagag tctcaccttc
     5041 cgatttgagt gtaatccggc agaggtacct gcccatctca tcggcggttt cgcccccaac
     5101 gaatcttaat ccaacttcaa gacgaccgag ggaatcggtc atccggctcg cgagtctttt
     5161 gccgtcaccg accgcaatcc aaccctcggg cccgtctgtc gaccaggcga gggtcttcga
     5221 agaggggcag tgcataaaat agattccctt gacctcgccc gcgggagcaa gaacgcccag
     5281 gtccataacc tcgggactca cctgcagggt gaccgccgcg cctgaatcat cctcggccgg
     5341 ggtgacacct gaaggtaaaa aggcaaggac gaccgatacg acgaacaaga cgatgatctt
     5401 tttt
//
LOCUS       DAOTMX010000094         5392 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_12767_length_5392_cov_3.886078, whole genome shotgun sequence.
ACCESSION   DAOTMX010000094 DAOTMX010000000
VERSION     DAOTMX010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5392
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12767_length_5392_cov_3.886078"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(224..1366)
                     /gene="sfsA"
                     /locus_tag="P5573_03795"
     CDS             complement(224..1366)
                     /gene="sfsA"
                     /locus_tag="P5573_03795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HRT27218.1"
                     /translation="MPRVAGKGRFVERLNRFVIICLLGSRRVRVHLPNPGRLQELLLP
                     GRTVYLAQNRGGNSTDFTAVAVEREGAPVLLHTHLANAVVGRLIEEGRLPGFENAHVV
                     GAEVACGRSRFDLLLERDGRPFVLEVKSCTLFGNSIAMFPDAVTVRGRRHIEELAALA
                     VKGTAAGVVFLVHTPRVRYFLPDYHTDLDFARTFLDVKDKLIIKAFSVGWKKDLTLCS
                     DVRELEIPWQLLGRELSDGGSYILVLHLADGLSIDVGGLGRVRFRQGFYLYAGSAKRN
                     LRKRIDRHLRKRKALHWHIDYLRAHAEVTAAIPVRASDDLEHALATAIGGIADWTHPG
                     FGSSDCDCPTHLFGMKTNPFRSPAFIDLLLHFRIDRLEQLIAEEKT"
     gene            complement(1489..2328)
                     /locus_tag="P5573_03800"
     CDS             complement(1489..2328)
                     /locus_tag="P5573_03800"
                     /inference="COORDINATES: protein motif:HMM:NF014074.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HRT27219.1"
                     /translation="MGIMDIFWLFFIISALQPVLQRRFLESMRTRKIAQIERRRGTRV
                     ILLVHRQETMSLLGFPLMRYIDVNDSEEVMRAIQMTDEDVPLDIVLHTPGGLVLAALQ
                     IARVIKSHKGKVTVFVPHYAMSGGTLIALAADEIVMCRHSVLGPIDPQLGQLPAASLI
                     KVVEEKPIAEIDDQTLILADVGRKAIRQVEAAATELLRKHMEEEKAREVAAKLASGTW
                     THDYPISADEARSLGLPVSTDMPSEVLELMTLYPQPVRRQGGGVEYLPIPRYREGQKQ
                     GNN"
     gene            complement(2319..3083)
                     /locus_tag="P5573_03805"
     CDS             complement(2319..3083)
                     /locus_tag="P5573_03805"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRT27220.1"
                     /translation="MFPIQDGVATRYPAMGTWALIAVNVVVFLFQVSLPPHALERFLS
                     AFALIPARYFGDLGATVPPIGMVDYLPFVSSMFLHGGWLHLILNMWTLWIFGPAVEDR
                     LGTFRFLVFYLIAGVSASFAHAVVNANSVVPTLGASGAIAGVIGCYVRKFPAARLVIM
                     VPVLIFPFFFEIPAVLFAFFWLLSQIIPGIAALMMPADGGGIAWWAHIGGFVVGWALA
                     PLVSRPARVYRRYYADEGPYGFLPNGRRTRRGQPWV"
     gene            complement(3236..3448)
                     /locus_tag="P5573_03810"
     CDS             complement(3236..3448)
                     /locus_tag="P5573_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HRT27221.1"
                     /translation="MSEKEKGVVKWFDEKKGYGFIARESGGDVFVHYSDIVGQAGFRS
                     LKEGDNVEFTIKQNQKGQAAANVMKV"
     gene            complement(3709..3963)
                     /gene="rpmA"
                     /locus_tag="P5573_03815"
     CDS             complement(3709..3963)
                     /gene="rpmA"
                     /locus_tag="P5573_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015818156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HRT27222.1"
                     /translation="MAHKKGQGSTRNGRDSNAQRRGVKVFSGQVVNAGSIIIRQVGTK
                     IHPGNNVGMGKDYTLYAKVDGVVQFERKDRNRKKVSVYAV"
     gene            complement(3976..4284)
                     /gene="rplU"
                     /locus_tag="P5573_03820"
     CDS             complement(3976..4284)
                     /gene="rplU"
                     /locus_tag="P5573_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041584674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HRT27223.1"
                     /translation="MYAVIETGGKQYRVSEGDLIEVEKIDGEKGVIVSFGALMVAEEG
                     DIRIGRPIVEGAVVRGEIVDHPRGPKITVFKMKRRKGFRKKTGHRQNLTCLRIKEISI
                     "
     gene            complement(4367..>5392)
                     /locus_tag="P5573_03825"
     CDS             complement(4367..>5392)
                     /locus_tag="P5573_03825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HRT27224.1"
                     /translation="ERKRLREIIEKLRPEKQGFIARTVSEGRGEEELRADVDYLTRLW
                     ETIKGVKDKVGVPGLVHREIGITLRAIRDIYTRDVSRIVVDSKKEYQRIVEFMKTFMP
                     KAHYYLEYYDKKEPIFDFFGIELDISRALNKKIWLKSGGYIVVETTEALTAIDVNTGK
                     YVGKRNPEETILNTNLEAVKEIAYQIRLRNIGGIIIIDFIDMQNEAHREKVFNALKEE
                     MGKDKCKTNILKISELGLIEMTRQRNGKNVDRILCDTCPYCEGKGLIKSKTTVCYEIF
                     REIEREGIRTLASTLCVLANPEIADLLLEDEKNTIERLEEKLKKKILVKVDKNFHQEN
                     FEIIPLQ"
BASE COUNT         1081 a         1579 c         1463 g         1269 t
ORIGIN      
        1 tcatcacgcc cccgccgccg cccgtgagca gcacataacc cttctccgcg atcgccgcgc
       61 ccaggcgatg cgcctcttcg gtcgtcttgg ggtctgcacg atggcctccc atgacaccga
      121 tgacgacggg ctttctcctc accgagattc cagccatgac aaaggccctc cgtttcgccc
      181 gggtttttat ccattaatga caatcggaga cggttgtcaa ggattatgtc ttttcttctg
      241 cgatcaactg ctccaggcgg tcgatgcgga agtgcagcaa aaggtcgata aaggcaggcg
      301 aacggaaggg gttcgtcttc atgccgaaga ggtgggtcgg gcagtcgcag tcggacgaac
      361 cgaaaccagg gtgcgtccag tccgcgatcc cccctatcgc cgtcgcgagg gcgtgctcta
      421 ggtcgtcgct cgcacggacg gggatagccg ccgtcacttc cgcatgggcg cgcaagtagt
      481 cgatgtgcca gtgcagggcc ttcctttttc tgaggtgcct gtcgatgcgt ttccggagat
      541 ttctcttcgc cgagccggcg taaagataga agccctgccg gaagcgaacc cggccgaggc
      601 cgcccacgtc gatggacagg ccgtccgcaa gatggagcac gaggatgtaa ctgcccccgt
      661 ctgacagttc ccggcccagt aattgccagg ggatttcgag ttctcggacg tccgagcaca
      721 gggtgaggtc ttttttccat cccacggaaa acgctttgat gatgagcttg tctttgacat
      781 cgagaaaggt ccgggcgaaa tcgaggtccg tatggtaatc ggggagaaaa taccgaaccc
      841 gcggcgtgtg aacgaggaag acgacgccgg cggccgttcc cttgactgcg agtgccgcca
      901 gctcttcgat gtgcctgcgt ccccggacag tcactgcgtc ggggaacatg gcgatgctgt
      961 tgccgaagag cgtgcaggac ttcacttcaa ggacgaaggg tctcccgtcc cgctccaaaa
     1021 ggaggtcgaa ccgactgcgg ccgcaagcga cttccgcccc cacgacatgc gcattctcga
     1081 agccgggcag gcggccttct tcgatcagcc gcccgacgac ggcattggca aggtgggtgt
     1141 gaagaaggac cggggctcct tcccgttcca cggcgacggc ggtgaaatcg gtgctatttc
     1201 cgccccggtt ttgggccagg tacactgtcc gtccgggaag gaggagttcc tggaggcgcc
     1261 cggggttagg gagatggacc cggacccgtc tgcttccgag caggcagatg atgacgaatc
     1321 ggttgagtct ctcgacgaaa cgtcctttcc cggcaaccct gggcagtcgt tggtttggtc
     1381 cgcttccctc tatattcttt cctgtctttc tttgtcttta tccgtcgcaa acagggtaga
     1441 cggcattttt ggtcgacttc cactaccgtt ctcggcacgc aagcactttc agttattacc
     1501 ttgcttttga ccttcgcggt agcgtgggat cggcaggtac tcgacgccgc ctccctgccg
     1561 tcgcacgggc tgcgggtaga gcgtcatgag ttcgagcacc tcgctcggca tgtccgtgct
     1621 gacgggaagg ccgagcgacc gagcctcatc tgccgatata ggatagtcgt gcgtccaggt
     1681 gccgctggcc agttttgccg ccacctcccg tgccttctcc tcttccatgt gcttgcgcag
     1741 aagttccgta gccgcagctt cgacctgccg gatggccttg cgcccgacgt cggccaggat
     1801 cagggtctgg tcgtcgattt ctgctatcgg tttctcctcg acgaccttga tcagcgaggc
     1861 ggcgggtaac tgtcccagtt gcgggtcgat cggcccaagt acggaatgcc tgcacatcac
     1921 gatttcgtcg gcggcaagcg cgatcaaggt gccgcccgac atggcgtaat gggggacgaa
     1981 aaccgtgacc ttccccttgt gagacttaat tacacgggcg atctgaagcg cggcgaggac
     2041 aagccccccc ggcgtgtgca gcacgatgtc gagaggcacg tcttcatccg tcatctggat
     2101 ggcgcgcatc acctcctcgg aatcgttcac gtcgatgtac cgcatcagcg ggaagccgag
     2161 aaggctcatg gtctcctgcc ggtgcaccag gagaatgaca cgcgtgccgc gccttcgctc
     2221 tatctgggcg atcttgcgcg tgcgcatcga ttcgaggaaa cgcctttgca ggaccggttg
     2281 cagtgccgag atgatgaaaa aaagccagaa aatgtccatt atacccatgg ttgacccctc
     2341 cttgttctcc tgccgtttgg tagaaacccg tatggacctt cgtcggcata gtagcgtcga
     2401 tagacccggg ccgggcgact gacgagcggc gcaagcgccc atccgacgac gaaaccgccg
     2461 atatgtgccc accaggcgat gccgccgccg tcagccggca tcatcaaggc cgcaataccg
     2521 ggaatgatct gcgacaacag ccagaagaat gcgaataaga cagcaggtat ctcgaaaaaa
     2581 aagggaaaga tcaggacggg aaccataatg acgagacggg cggccggaaa tttcctcacg
     2641 taacatccaa tcacaccggc gatggcgccc gaagcgccga gagtcggcac aaccgaattc
     2701 gcgttgacga ctgcgtgggc aaaagacgcg gagacgcccg cgatgaggta gaaaaccaga
     2761 aaacggaacg tccccaggcg atcctcgaca gcagggccga atatccacag cgtccacatg
     2821 ttgaggatga gatgcaacca tccgccatgg aggaacatgc tggagacgaa gggcagatag
     2881 tcgaccattc ctattggagg cacggttgct ccaaggtcgc cgaagtaacg ggccggaatt
     2941 aaggcaaacg ccgagagaaa ccgctccaag gcatggggtg gaagcgacac ctggaacagg
     3001 aacacaacca cgttcacggc aatcagtgcc caggtcccca ttgccgggta gcgcgtagcc
     3061 acgccgtctt ggatcggaaa cataacctcc ctgctctcgc ttggcattaa attccacggg
     3121 gaggcgaggg cagcacctcg atcccgtgaa gcgcttcatg gtgacagtat cccctgccga
     3181 ctgcaagggc gggaacggcc cggggtggat atgccggtat tcgaagtgga aaccgttaaa
     3241 ccttcatgac gttcgcggct gcctgtcctt tttggttttg tttgatggtg aactcgacgt
     3301 tatccccctc tttcaagcta cggaagccgg cctggccgac gatatcggag taatggacga
     3361 agacatcgcc ccccgactca cgggcaataa agccataacc ttttttctca tcgaaccact
     3421 tcacgactcc cttctccttc tcgctcatca aaaaccctcc tttttgttgc tgcactaccc
     3481 atgataccga aaaaatttac atccccgaat aaaaaaaccg cttggccggt cacagctcaa
     3541 gcggtctatc cttcacgtcc ttgataccct gaaaaaactg ttcttcgaaa tcataatcat
     3601 tgaagcggtc aacccgctgc agtgactcta ttaaaaaagt taagcagcgt caagaaaata
     3661 aaacggccgc ttcaattgcg atagggagcc gacggggatg aatcgttcct acacagcgta
     3721 cacgctgact ttttttctgt ttctgtcttt tcgttcgaac tggacgacac cgtcgacttt
     3781 cgcgtaaagt gtgtagtcct ttcccatgcc cacgttgttt cccgggtgga tcttggtccc
     3841 cacctgtctt attatgatgg agcccgcgtt gacaacctgg ccgctgaaca ccttgacgcc
     3901 ccttctctgg gcattgctgt cccggccgtt gcgcgtgctt ccttgtcctt ttttatgtgc
     3961 catctcgccc tcccgctaaa ttgaaatctc cttgatcctg agacaggtaa gattttgccg
     4021 gtgccctgtt tttttccgga aaccctttct ccttttcatt ttaaaaaccg tgatcttagg
     4081 ccccctggga tggtccacga tctcgccgcg caccacggct ccttccacta tgggcctgcc
     4141 gatacggatg tcgccctctt cggccaccat tagggccccg aaagagacga tgacgccctt
     4201 ctcgccgtcg attttttcca cttcgatgag gtctccttcg gagaccctgt actgttttcc
     4261 gcctgtctct atgactgcat acatctttcg gacaaccttc ctggtagatt gtttaacata
     4321 aagtttacct taatacaaaa attcatatgt ttttgtcaat caaaattcac tgcaacggga
     4381 ttatctcgaa attctcctgg tggaagttct tgtccacctt gacgaggatc ttctttttca
     4441 gtttttcttc cagtctttct atcgtattct tttcgtcttc cagcaggagg tcggcaatct
     4501 ccgggtttgc gaggacgcag agcgtggagg ccagggtacg gatgccttcc ctttcgatct
     4561 cacggaagat ttcgtagcag accgtggtct tggatttgat caggcctttc ccctcgcagt
     4621 aagggcaagt gtcgcagagt atgcggtcta cgttcttccc gtttcgctgc cgggtcattt
     4681 ctatgagccc caactcggat atcttcagga tgtttgtctt gcacttgtct ttgcccattt
     4741 cttccttgag ggcgttgaag accttttccc ggtgggcctc gttctgcatg tcgataaaat
     4801 cgattattat gatcccgccg atattccgga ggcgtatctg gtaggcgatc tcttttacgg
     4861 cctcgaggtt ggtgttcagg atcgtttctt ccgggttcct ctttcccacg tactttccgg
     4921 tgttcacgtc gatcgcggtc aaggcctccg ttgtctctac gacgatgtaa ccccccgatt
     4981 tgagccatat ctttttgttc aaagcccgcg agatgtcgag ctctatgccg aaaaagtcga
     5041 agatgggctc ttttttgtcg taatactcga ggtaatagtg ggccttcggc atgaaggtct
     5101 tcatgaactc gactatgcgc tggtattctt tcttcgagtc cacgacgatc cggctcacgt
     5161 cccgggtgta gatgtcccgg atggctcgca gggttatccc gatctctcga tggacaaggc
     5221 cggggacgcc gaccttatcc ttcaccccct tgatcgtctc ccataggcgt gtcaggtagt
     5281 ccacgtcggc ccgaagttct tcctcgccgc gaccctcact caccgtcctt gcgatgaagc
     5341 cttgcttctc gggacggagt ttttctatta tctcccggag gcgcttcctt tc
//
LOCUS       DAOTMX010000095         5380 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_12797_length_5380_cov_4.035305, whole genome shotgun sequence.
ACCESSION   DAOTMX010000095 DAOTMX010000000
VERSION     DAOTMX010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5380
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12797_length_5380_cov_4.035305"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="P5573_03830"
     CDS             complement(<1..765)
                     /locus_tag="P5573_03830"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRT27225.1"
                     /translation="MAETDRSASLPLYGIKKVLLQLVDTRVLILFVAPLVLYIAFSHF
                     MPLIEPDESRYFEIADNMLDSGDYVTPRLSGVIYLEKPPLSYWASAFILHIFGENDFT
                     ARLYVGICAWACLLLVYSIGSVLYDRKTGLYASGVLGTTLFFFVFGNFNILDIPLTFY
                     TCSAIWAGYRYLTGTIGNRSWLYLLYLSSALAFLTKGLIGVVFPFAILSLWLAISGQG
                     KRILKLFSLLGIALFLAITCPWLILIQKEHTDFFRFF"
     gene            complement(1109..2509)
                     /locus_tag="P5573_03835"
     CDS             complement(1109..2509)
                     /locus_tag="P5573_03835"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRT27226.1"
                     /translation="MSLRKAFEIRRTLVFRLTFWYALVYIVSSLAAFAVFYIVIANEV
                     RERTDQEMLRELAEAASILKSKGEDSFRAEMDFEAESEGVGDIFFRLLDRAGGEVAET
                     DMTAWKGVGINLNALARVAAGGGPVFETVAVPGKPYEARIIYGLLESGRIIQIGCSLE
                     HDAAFLGTIQDMVMPGILVVVALSTLTGWFMAARALAGIKDVTRTALDISEGAFEKRV
                     NIQARGEEVKQLAVAFNRMLDRIHELVKGLREVTDDIAHDLRTPITRIRGFAEAELIA
                     GKSGEESNKLAADTVEECDNLLQMINTMLEISEIEAGISVLSKAEVDLVQLVGEALEL
                     FRPIADEREVRLVFHGPAHLSVQGNVHGLQRIVVNLLENAVKYTPGGGCVTVALRGDE
                     HAVSIIFRDTGVGIAEEDLPLVFNRLYRCDSSRSQPGFGLGLCLASAVARAHGGRIAA
                     ESKLGEGSTFTVTLPR"
     gene            complement(2487..3185)
                     /locus_tag="P5573_03840"
     CDS             complement(2487..3185)
                     /locus_tag="P5573_03840"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRT27227.1"
                     /translation="MARTRSTMRLLLVEDDLKIASFVLKGFREAGFTVQQAKDGETGL
                     HMALHEPFDAAIIDIMLPKLDGLSLIQRLRLKKVDMPVIILSARTSVDDRVKGLQSGG
                     DDYLTKPFSFSELLARVQALIRRATGAREATRLVVGDLSLDLLKREVYRAGKRIDLQP
                     REFSLLEYLMRNEGRVVSKTMIMQNVWNYDFVPMTNVVEARMCILREKIDKEFSPKLI
                     HTVRGAGYVFKEGV"
     regulatory      3358..3549
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3702..4424
                     /locus_tag="P5573_03845"
     CDS             3702..4424
                     /locus_tag="P5573_03845"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HRT27228.1"
                     /translation="MSVKESVGGSLRKNWTKCIAASLLIHGAALFIPFPVMVTRMAGP
                     IEVFMLEGPGSSAPSGGETKKQDSPRGKPRLEMRRPQAREEARLRPQETSTTVEQKVG
                     PAQTVTENITVHESSTRNAPASGIGLESGAAGGSGRGNGAGERNTGFGSPSGPKFLHR
                     EIPEYPFLARKRKKEGKVILAVVIDATGRLTGAEVIEASDRMFAEASLEALKKSTFLP
                     ARRNGRPVTSRAILPIRFSLTE"
     gene            4467..5138
                     /locus_tag="P5573_03850"
     CDS             4467..5138
                     /locus_tag="P5573_03850"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRT27229.1"
                     /translation="MWHILVKGGWMMIPIVLCSLVAVATIIDRFLFFRRIRKKNRGEE
                     VIELVREGQIKNARQIVESEKIQSGLPIMKVLYAGITHPVEPEGAMEAAAVVEVSAMK
                     RGLAALDTIITLSPLLGLLGTVIGMINAFNVMASSGLGQPHAVTGGVAEALICTAAGI
                     SVAVVALIPYNYFLARIERETDRIETMATRLELALGVSAGAQRRPLIESREADLHGSR
                     QGVSV"
     gene            5135..>5380
                     /locus_tag="P5573_03855"
     CDS             5135..>5380
                     /locus_tag="P5573_03855"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRT27230.1"
                     /translation="MKLKRHLTRKARIEIIPMIDVVFFLLVFSMLTSLAMATIGAPPV
                     NVPEVQTSEKGSQIRVFVTLTRDKGLYVNDKQIKATEL"
BASE COUNT         1302 a         1503 c         1493 g         1082 t
ORIGIN      
        1 aaagaacctg aaaaaatccg tatgttcttt ttgaatcaga atgagccacg ggcatgtgat
       61 ggccaagaac aacgctattc ccaaaaggga gaacaacttt aggattcgtt tcccctgccc
      121 gctgatggca agccataagc tgagaatcgc aaacgggaag accacaccga tcaacccttt
      181 ggtcaagaag gccagggcgc tggacagata aaggagatag agccatgacc tattaccgat
      241 agtgccggtc aaatagcggt atccagccca gatggccgag cacgtataga aagtcaaagg
      301 gatgtcgagg atgttgaagt ttccaaagac aaaaaagaag agagtggttc ccagcacccc
      361 cgatgcataa agcccggtct ttctgtcata aagcacagaa cctatggaat agaccaacaa
      421 cagacaggcc caggcgcaga tacctacata aagcctggct gtaaaatcgt tctcgccgaa
      481 gatatgtaga atgaaagcag aggcccaata cgaaagggga ggcttttcca agtatatcac
      541 accggacaga cgcggcgtga cataatctcc ggaatccaac atgttgtcag ctatctcaaa
      601 ataacggctt tcatcaggtt ctatcagcgg catgaaatgc gagaaggcaa tataaagaac
      661 gagcggcgct acgaatagaa tgagcacccg ggtatcgacc agttgcaata aaactttctt
      721 aatcccgtaa aggggaaggg acgcgctacg atcggtctct gccaaaatgt acccccttgg
      781 tagctaaaca accgttcatg aaaccactgc cgcctcatgc cgagccttgc ttttacccga
      841 gttcaatgcg cccgcctgag gcacagccat cgagagccct tgccgcgccg tgccagcaaa
      901 ggcgcggctc aatccaccac ctttgcctgc ttgtctgtcg cgtatccttc aacgtatcgc
      961 ctgaaggata aacaggtttc ccgctgggaa aggctctatt ttttcgttta tggcacagtc
     1021 agattatggg aagattatcg gaagattaag atttcgtaat gttggcatgg gaaatggcat
     1081 ttcttggatg gatttacggg gtacaatcct aacggggtaa cgtgacggtg aaagtgcttc
     1141 cttctcccaa cttgctttcc gccgcgatac ggcccccatg ggcgcgggca acggccgatg
     1201 caaggcagag gcccagtccg aatcccggct gagaacggct gctgtcgcac cggtaaagcc
     1261 ggttgaagac caggggcagg tcttcttcgg caatgccgac ccccgtgtca cggaagatga
     1321 tactgacagc atgctcgtcg ccgcgcaggg caacggttac gcatccccct ccgggcgtgt
     1381 acttaacggc attttccaag agattgacga cgatccgctg aaggccatgc acgttgccct
     1441 gaacagacag atgtgcgggg ccgtgaaaga caaggcggac ttccctctcg tcggcaatcg
     1501 gccggaagag ctccagggcc tcgccgacga gttgcactaa gtccacctcc gccttggaca
     1561 gtacactgat gcccgcctca atctcggaaa tctccagcat ggtgttgatc atctgcaata
     1621 ggttgtcgca ttcttccacg gtgtccgccg caagcttatt ggactcctcc cccgatttgc
     1681 cggcgataag ttccgcttcg gcaaaccccc gtatccgggt aatcggggtc ctcaggtcgt
     1741 gagctatgtc gtctgtcact tccctcagac ccttcaccag ttcgtggatg cggtccagca
     1801 tccggttgaa ggcaacggcg agctgtttga cctcttcacc cctggcctgg atattcaccc
     1861 gcttctcgaa ggccccctcc gagatgtcca gtgccgtccg cgtgacgtcc ttgataccgg
     1921 ccagggcacg ggccgccata aaccacccgg tcagcgtcga aagggctacc accaccagga
     1981 tacccggcat gaccatatcc tggattgtgc caagaaaagc ggcgtcatgc tccagggaac
     2041 agccgatctg gatgatccgg cccgactcaa gaagtccgta gatgattcgt gcctcgtacg
     2101 gcttcccagg aacggcaacc gtttcaaaca cggggccgcc cccggccgcc acgcgcgcca
     2161 gggcattcaa gttgatacct acccctttcc aggcagtcat gtccgtttcg gcgacctcgc
     2221 cgccggcccg gtcgagcagg cgaaagaaga tgtccccaac gccctcggat tccgcctcga
     2281 agtccatttc ggccctgaaa gaatcttcac ccttcgattt caggatggaa gccgcctcgg
     2341 cgagctctct cagcatctcc tgatccgttc tttccctgac ttcgttggca atgacgatgt
     2401 agaagacggc aaacgccgcg agagaggaaa cgatgtagac taacgcgtac cagaaggtaa
     2461 gccggaagac cagcgtccga cgtatctcaa acgccttcct taaagacata ccccgcacca
     2521 cggaccgtgt ggatgagctt cggactgaat tccttgtcga tcttttcccg caggatacac
     2581 atcctggcct ccacgacgtt cgtcatagga acgaagtcgt agttccacac attctgcatg
     2641 atcatcgtct tggaaacgac ccggccctcg ttgcgcatca ggtattcgag aagcgagaat
     2701 tccctcggtt gcaggtctat cctctttccc gcgcggtaca cctcccgttt gagtaaatcc
     2761 agggagaggt ccccgacgac aaggcgggtc gcttccctcg cccccgtcgc tctccggata
     2821 agcgcctgca cccgcgccag caactcggag aaggaaaacg gcttggtcag gtagtcgtcg
     2881 ccgccgcttt gcaggccctt gacgcggtca tccaccgaag tcctggcact taggatgatc
     2941 acgggcatgt ccaccttttt gagacgcaac cgctgaatga ggctcaggcc gtcgagcttg
     3001 ggcagcatga tgtcgatgat ggcggcatcg aaaggctcgt gcagcgccat gtggaggccg
     3061 gtctccccgt ctttggcctg ttgcacggtg aagcccgcct ctcggaaccc cttcaggacg
     3121 aaagaggcaa tcttcaggtc atcttcgacg agaagaaggc gcatggtact ccttgtacgg
     3181 gccatcagaa atacagatgg tttaaaggat taggattcca tcatggaacc cgctgatttc
     3241 catccgatat gtagcataaa caagaatcgg tgagaagaat aaaacgtgcg gcgaaccatt
     3301 tatcggcgat gaaaatcctc gaccaaaaaa aaacttgacg ccaactgcat atttgcttta
     3361 tgtaggttgc aaacaagagg ccgaacaggc cataaaaggg aagacggtga aaatccgtcg
     3421 cggacccgtc gccgtaaccg gggatgaaag ccgcaggtaa tgccactgtt ccttacaaac
     3481 ggaatgggaa ggcgcggcga gtaagatgat ccggaagccg gaagaccttc ttgttggttt
     3541 aatgtgaact gtcctccgtg gtgaagaggg tggttgcagt aacatactgg atggaaaacg
     3601 gcatccccgg acttgagcaa tatcaggtcc ggggattttt ttatgcgatt gcggatcaac
     3661 agggtatccg gacgaggctt taggtgaggg agggcgcgca gttgtccgtt aaagagagtg
     3721 tcgggggaag cttgagaaaa aattggacaa agtgcatcgc tgcctcgctg ctgatccatg
     3781 gagcggcgct cttcattcca tttccggtca tggtcacgcg aatggccggg ccgatcgagg
     3841 tttttatgct ggaggggccg ggctcctctg ctccttcagg cggcgaaaca aaaaaacagg
     3901 acagtccacg gggcaagccg cgcctggaga tgcgcaggcc gcaggcaaga gaagaggccc
     3961 gcctacggcc gcaggagaca tcaacaacgg tggagcagaa ggtgggaccg gctcagactg
     4021 tgacagagaa catcactgtt catgaatctt ctacccgtaa cgccccggca agcgggatag
     4081 ggctcgaatc gggcgccgca ggcggttccg gccgggggaa cggcgcaggt gaaaggaata
     4141 caggattcgg ctctcccagc ggaccaaagt ttctgcatcg agagataccg gaatatccct
     4201 ttctggcaag aaaaagaaaa aaagaaggaa aggttattct tgcggtagtc atcgacgcga
     4261 cgggaagact taccggcgcc gaggtcatcg aggcatccga caggatgttt gccgaagcct
     4321 ctctggaggc gttgaagaaa tcgacttttc taccggccag aagaaacggc cggccggtga
     4381 catccagagc aatcttgccg atacgctttt ccctcacgga gtgagtggcg tgacccttcg
     4441 atcgtttact gaaagaggag gacaagatgt ggcacattct cgtaaaaggc ggatggatga
     4501 tgatccccat cgttctctgt tcgctcgtag cggtggcaac cattatcgac cggtttcttt
     4561 tcttcaggcg catcagaaag aaaaatcggg gagaagaagt catagaactt gtccgcgaag
     4621 gacagattaa gaatgcccgg cagattgtcg agtcggaaaa aatacaaagc ggtctgccca
     4681 tcatgaaggt tctttacgcg ggcattactc atcccgtcga accggaaggc gccatggaag
     4741 ccgcagccgt cgtcgaggtg tcggccatga agcggggtct cgcggcgctc gacacgatca
     4801 tcacactctc gcctctactc ggcctgctgg gcactgtcat cggcatgatc aacgccttca
     4861 atgtgatggc ctcctccggt ctgggacagc cccacgccgt taccgggggt gttgccgagg
     4921 cgcttatctg cacggcggcg ggaatttccg tggcggtcgt ggccctcata ccctacaact
     4981 attttctggc ccgcatcgaa cgagaaacgg atcgcatcga aacaatggca acacgcctcg
     5041 aactggcgct tggcgtctcc gccggcgcac agcgccggcc gctcatagaa agccgagaag
     5101 cggacctgca cggctcaagg cagggggtat cggtatgaaa ctgaaacgcc acctcaccag
     5161 gaaggcccgc atcgagatca tcccgatgat tgacgtcgtc ttcttcctgc tcgtgttctc
     5221 catgttgacg tctctggcca tggccaccat tggcgcgccg ccggtgaatg taccggaggt
     5281 gcagacctcg gaaaagggca gccagatacg cgtgttcgtg acgctgaccc gcgacaaagg
     5341 cctctacgtt aacgacaagc agatcaaggc gaccgaactg
//
LOCUS       DAOTMX010000096         5369 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_12826_length_5369_cov_3.583365, whole genome shotgun sequence.
ACCESSION   DAOTMX010000096 DAOTMX010000000
VERSION     DAOTMX010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5369
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12826_length_5369_cov_3.583365"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(202..591)
                     /locus_tag="P5573_03860"
                     /pseudo
     CDS             complement(202..591)
                     /locus_tag="P5573_03860"
                     /inference="COORDINATES: protein motif:HMM:TIGR01909.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
     gene            complement(685..1071)
                     /locus_tag="P5573_03865"
     CDS             complement(685..1071)
                     /locus_tag="P5573_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27231.1"
                     /translation="MEGTCAVRTPRSFAVEIPGSARGKDGEKCCPTLVKENHPADRIG
                     GSREAHIGSLARASLERESIQAIEKAGFDHIRIIDETHIPVELAVNGPAVPTIMEKPN
                     QASENLRGLARTVVSIGIQALKPEAI"
     regulatory      1335..1427
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1733..2593
                     /locus_tag="P5573_03870"
     CDS             1733..2593
                     /locus_tag="P5573_03870"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRT27232.1"
                     /translation="MKRITASSRLVGVLAAGILIVLAVFSAMTLDYIFIKRDRAEVLS
                     LRELTEAQETRIATLSERIGQYEQTLAVLHDFDAKLRVLAREVNKKAPAALKPPSRVS
                     FSRLGIGGSTPEGDLGKLETENLNRQMDRLIEEAAIREQSFRDLIEFFKKQRSVLACV
                     PSIWPVRGWVSSEFGIRQSPFTGRREFHKGIDIAADPGREIVAPADGIVVLNGRDPNM
                     GLVVQLNHSQGFSTIYGHLLRSVVRKGQILRRGDVIGFIGNSGRSTGPHLHYTMLLNG
                     VPVNPRKYLN"
     gene            2993..3637
                     /locus_tag="P5573_03875"
     CDS             2993..3637
                     /locus_tag="P5573_03875"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HRT27233.1"
                     /translation="MKKGAVSLLFLILLAGLVAFPVTGSHALTLSVGDVITFQDGLGP
                     SPGGSFQVYKDGQFAYNSFCLETNEYISFNTQYRVVGISDTVYQGGSNTSSGDPLDPR
                     TAYLYYHYMIGDLSQLASSFDPTSSASLGDLQNAIWYIEQEYLGVNNYLVALADRSGW
                     TDIGPVRVLNIKTLNGGNAQDQLYYAAVPEPATLLLLGCGLAGLITFRRKNPRG"
     gene            3885..4388
                     /locus_tag="P5573_03880"
     CDS             3885..4388
                     /locus_tag="P5573_03880"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRT27234.1"
                     /translation="MVKITEKDKTILVIEDDAVTREMIKNTLAENGYEVSTAFDGIDA
                     VLCLTKRNFDLILCDIFMPNLNGFQLLEFLKRNELSIPVILVTASDNTEDEVRGLMLG
                     AEDYIRKPINKDTLLLRVGKILDHSLERIDHISFGRSTLESQEVSKKTTIGPGTEKKG
                     HNNPSRA"
     gene            5051..5200
                     /locus_tag="P5573_03885"
     CDS             5051..5200
                     /locus_tag="P5573_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27235.1"
                     /translation="MGYTKTCVVCGRATWMLNDEDKCEQCAKKEEKSAQTPTSSTGTT
                     PEQRR"
BASE COUNT         1245 a         1518 c         1373 g         1233 t
ORIGIN      
        1 gcggggctcg agccctgcca cgcctgcgat ccgttcgccg tccctgacca ccaggaaatg
       61 ctccagccgg gccgttttga tatcgccgtg gggaagcccc gcttcgtgaa gcagggcctt
      121 gatcttcggc cagtccccgt atgctgcaac gtcaatcttc accccgtaag actccaccgc
      181 ccgggtgaaa taatgccccg cctactcctt cgattccgcg gaatcgagca cctcccccat
      241 gatccgggcc gccttgccca caatttccac gcactgcaga aatttgtcac cctcctggta
      301 gtccctcacc tgcttcgggt ccgcccagtc cacgtccacg aaatcgcggc agaggatgct
      361 tccacctccg acctcctccc ggaaccgctt catgaaacgg aaggcgcagg cccacatctt
      421 cctatcggcc ctttcgtccc cgttcatccg gccgtaacga aggctcagcg acgcaagccc
      481 gccgatgacg gcaccgcaga cctccccgtg ggcccccagg cccccgccga atacatccat
      541 gaccttcatg aggtttgaat ccgcaagccc gagtttttct tgcccgaccg caagaacggc
      601 ctggcttcag tgatagttgt tgaagaacag cgtccgcgcc ttttcctgca tttcctgcgg
      661 tgtcatgtcg ctcctcctct gcgctcaaat ggcttcgggt tttaaagcct ggattccgat
      721 actcacaacc gtgcgggcaa gccctctcag attctctgaa gcctggtttg gcttttccat
      781 gatggtcggc acggcggggc cgttcacggc caattcaaca gggatatgtg tctcatcgat
      841 gatccggata tgatcgaacc cggccttctc aatggcttgg atggactccc tctcgaggct
      901 cgcccgggca aggctgccga tgtgggcctc gcgggaaccc ccgatccggt ccgccggatg
      961 attctctttc accagggtgg gacagcattt ctccccgtcc ttcccccggg ccgaccccgg
     1021 gatttccacg gcgaaagacc tcggcgtcct cacggcgcag gtcccctcca cggaagccgt
     1081 aaaacaggat ggccgcttct ttttctttat gccggaatcc tccgacgcgc cggattcaga
     1141 ctgattacca tattctgatt gttcagcata cgctgaactc tatgcatccg ggatgaaagg
     1201 tcaagtgaaa atacacgatg gcttccaacc tctcgcatcg acagcgtaat ttcatgaacc
     1261 gcacccgtgt gcagacagcc ttgccgactc aagttttccc ttgatcggac gatatatatg
     1321 atgaagaaaa cgtcgtttga aaaaggcaaa ccagccgaaa gactgggacg caaagccact
     1381 ggcctacatt ccggcggaaa ccgggacagg gcagcagggt tgccaggcga tgactttatt
     1441 ccgcctttgc cggcgggtgc aaggcatctc cccacgggtg cggctccgaa gccggcatca
     1501 cattccaccc cttccgtaac aaccccgctg ccgatctttg cgctgaagct tccccgcgga
     1561 gggtctgaga tgaaccacca ccctgtgagt cccccggccc atgaagacgg ggcgccgttc
     1621 ggcaacggcg ttttccgggc gggctgaacc ggaagtatcc tgtctgcgaa gcgatgcaat
     1681 aatgaagaat gattgttaca cctttttgat cgttccgcgg agaaggtcgt ccgtgaaacg
     1741 gatcaccgcg tcgagccgcc tcgtgggggt tcttgcggcc ggcatcttaa tcgttcttgc
     1801 cgtcttctcc gccatgaccc ttgattacat cttcatcaaa cgggacaggg ccgaggtgct
     1861 cagcctgaga gaactcaccg aggcccagga aacccggatt gcaacgctca gcgaaagaat
     1921 cggccagtat gagcagactc tggcggtact gcatgacttc gacgcgaagc tccgcgtatt
     1981 ggcccgtgag gtcaacaaaa aggcccccgc agcgctcaaa cccccatcgc gggtctcctt
     2041 ttccaggctg ggtatcggtg ggtccacccc ggaaggcgac ctcggaaagt tggaaaccga
     2101 gaacttgaac cgacaaatgg accgcctgat cgaggaggcg gctatccggg aacagagctt
     2161 ccgggacctc atcgaattct tcaaaaagca gaggtccgtc ctcgcctgcg tgccctccat
     2221 ctggcccgtt cgggggtggg tgagttccga attcgggata agacaatctc ctttcacggg
     2281 ccgccgcgag ttccacaagg ggatcgacat cgcggccgat cccggcaggg agatcgtcgc
     2341 tcctgccgac ggcatagtcg tgctcaacgg gcgggacccg aacatgggcc ttgttgtcca
     2401 gttgaaccat tcccagggat tctctaccat ctatggtcac ctcctcagaa gcgtcgtccg
     2461 caaaggccag atcctgcgca ggggcgacgt catcggtttt atcggcaact cggggcgaag
     2521 cacggggccc caccttcact acacgatgct gctaaacggc gtcccggtca acccgcgtaa
     2581 atacctgaat tgagggatgg ggctcaccat cattttcatc ttgaaatacc gcgtgagcac
     2641 gctgtcctga gggtatttat caacaggcag atcggttata tcgtttcaaa agcgtcggat
     2701 tgcccccttt ttttctactc gtcgtttctc ccatacactg agccgtaaac tgaacggtaa
     2761 tgacatgtga caattaattt aatgattaca aattattatc ttatattcct acgaaaaaag
     2821 tattagatat ccacagaaaa ttttaatact tttttatgac atgattattt ttgaaattag
     2881 atgcaaattc atacctttta catattgact ttgactattt tatctgtaat atggttgcat
     2941 atcgtacata tgaaaccaat gcatcatgac agttgatagg agggaacaaa ggatgaaaaa
     3001 aggagccgta agcttactgt tcttgatctt gcttgccggt ctggttgcat tccctgtcac
     3061 cgggtcgcac gctttgacac tgagcgtagg cgacgtcatc accttccagg acggtcttgg
     3121 tccctcaccc ggaggctctt tccaggtcta caaggatggg caattcgcat acaacagctt
     3181 ctgccttgaa acgaatgagt acatctcgtt caacacccag tatcgagtgg tcggcatatc
     3241 ggacacggta taccagggcg gttcaaacac gagtagcggc gatccccttg atcccaggac
     3301 ggcctatctg tactaccatt acatgatcgg agatctcagc cagctggcgt caagtttcga
     3361 tccgacatcg tcggcaagcc tgggggatct gcagaacgcc atctggtaca ttgaacagga
     3421 atacctgggg gtgaataact acctggttgc gcttgccgat cgctcaggct ggacggatat
     3481 cggccccgtt cgggtgctca acatcaagac gctgaacggc ggaaacgccc aggatcagct
     3541 ttattatgca gctgtcccag aacctgcgac actccttctt ctcggctgcg gcctggcagg
     3601 gctcattaca tttcgcagga agaatccacg cgggtaatgc acgatcctac aatcaccgaa
     3661 accggaagcc ccgtgatgtt gtaatcccgg ggctttttac ttgatcgccg ggatcaacaa
     3721 tccggattca gtcgccgtag atgcgttttt aaatcgttca cctcaactgt ggagacggcc
     3781 tggaaggacc gaaagcaatc tgcgcaaggt cggacaaagg gtcgtcagac ggcatccggg
     3841 tggagcagac ttgcagcggc ctttgatcgt gctgaacgct catcatggtt aaaataacgg
     3901 aaaaagataa aaccatactg gtgattgaag atgatgctgt cacccgggaa atgatcaaaa
     3961 atactctggc ggaaaacggc tatgaagtct caacggcttt cgatggaatt gatgccgttt
     4021 tgtgcctcac gaaaagaaat ttcgatctga tcctgtgcga catcttcatg cccaatctca
     4081 atggtttcca gctgctggaa tttctgaaaa gaaatgaatt gagtatacct gttatcctgg
     4141 tgaccgcgtc agacaacaca gaagatgaag tcaggggatt gatgctggga gcagaggatt
     4201 atatcaggaa acccatcaat aaggatactt tactgctccg cgtgggaaaa attcttgatc
     4261 attccttgga aagaatagat cacatctcgt tcggccgttc gactcttgaa tcacaagaag
     4321 tgagcaagaa aacgaccatc gggccgggca ctgaaaagaa aggacacaat aatcccagcc
     4381 gagcatgacg gggaccaggt cctccgctgt gagtaaggca ccgcggcttc ccgcagaaag
     4441 aatccggcga ggaatattct caatacagcg acagaagcta aaaagtcccg gggttacaca
     4501 ggttttcggg atctttttaa tttgttcgcc aaagaaaaga ctcggatcct gaggttttat
     4561 cacctgcctc tttgatgctc ttctctctgc gatttacccc tccggacgca aatgattcaa
     4621 gacttacccg cgaaacagtg ccgaacaaca gctcaactga agagcacccc tgccggtcct
     4681 gaactttaaa tatcgattaa tatggaaaat tacggatgga atgcagtccg gtctgcatga
     4741 acttgtcagg aagcgttccc gtttgcactt ttccgctttg ggttcttctg cagaaatgaa
     4801 ccctgcgggc acaatatgtt cccttttagc cgtgaagccc cttgactttc cctgccgggc
     4861 atttatactg ttaatagaat attgaaagcc cgcctacttt tggggttctt ctccgaatac
     4921 agatgtgatc ctgtttactc gccttctttc ctcagccgcc tgtttctaaa cgacctgaga
     4981 cactcgttgc ttttcggccg gttacccccg gcgggcaaat tgcccccaag gggatcgagg
     5041 aggtgtcgac atgggataca caaagacgtg cgtcgtatgc ggacgggcaa cgtggatgct
     5101 caacgacgag gacaagtgcg agcagtgtgc caagaaggaa gaaaagagcg cgcaaacccc
     5161 gacctcatca acgggaacta caccggaaca gcgccgctga acggattgaa cgcagtttga
     5221 ttgaagcggt ctcttcgtcg gtctggcctt tttaaaggct gaatggggaa gtgtgggctt
     5281 ttgtcccaaa atcgcagagt ttgttccttc gtcatcgaag aaggcagagc cgggcgactc
     5341 tgccttcttc atttctcatc attgtcgat
//
LOCUS       DAOTMX010000097         5360 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_12853_length_5360_cov_3.574364, whole genome shotgun sequence.
ACCESSION   DAOTMX010000097 DAOTMX010000000
VERSION     DAOTMX010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5360
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12853_length_5360_cov_3.574364"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="P5573_03890"
     CDS             complement(<1..206)
                     /locus_tag="P5573_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27236.1"
                     /translation="MLLICGTIPDEDFPLTIGPVAFDEESLFLDGKALSCTQGTAALV
                     GAACKVTAYLGTEAPRALLVGAAEG"
     gene            complement(208..744)
                     /locus_tag="P5573_03895"
     CDS             complement(208..744)
                     /locus_tag="P5573_03895"
                     /inference="COORDINATES: protein motif:HMM:NF023251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HRT27237.1"
                     /translation="MVFDFKKKGQSEGGIILFNNVEEAIAAERLLMAAGYEARLVAPP
                     PALRKGCDLAVEINLVEQTGIERLFAEKKAGYLEILPVKGKGALLGVVKVTDFGEAVM
                     VKAGNMKLTFEKESGRVVNVSGGGCPDIPYLHACLIDKKLTEARRPKEYGYTLCSLML
                     DRAFIESLDIWKGNGGRS"
     gene            complement(744..959)
                     /locus_tag="P5573_03900"
     CDS             complement(744..959)
                     /locus_tag="P5573_03900"
                     /inference="COORDINATES: protein motif:HMM:NF013379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HRT27238.1"
                     /translation="MSETIDTRGLSCPEPVILVKRAIDRGSFPIEVTADSQTAKENVT
                     RMAVHSGCTVTCETHGDTFLLRIDRGV"
     gene            complement(975..2087)
                     /gene="yedE"
                     /locus_tag="P5573_03905"
     CDS             complement(975..2087)
                     /gene="yedE"
                     /locus_tag="P5573_03905"
                     /inference="COORDINATES: protein motif:HMM:TIGR04112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YedE family putative selenium transporter"
                     /protein_id="HRT27239.1"
                     /translation="MKDFLSSSTGIVTAGGIIGAIAAYLVYLGNPPNMGVCVACFTRD
                     TAGAIGLHRAGAVQYIRPELVGFVFGSLLSALAFREFRPRGGSAPLIRFVLGFFAMVG
                     ALAFLGCPWRALARLAGGDLNAVVGIAGLAAGITVGVWFLKTGYSLGRSAPNRTAVGL
                     VMPAFMLFLFLLVALEVKFTSDGPVFFSSAGPGSMHAPLFLSLGAGLLVGVLAQRTRF
                     CTMGALRDVILIKDFHLLSGVIAFVVAAFAVNFLIGKVNIGFAQQPIAHSNHLWNFLG
                     MCLSGLCFALAGGCPGRQFFMSGEGDADATVFIFGMLMGAAFAHNLDFAAAPDKFDRA
                     AQLLVIGGPSAWGQSAVLIGLVITLAIGFSMRERIR"
     gene            2231..3205
                     /locus_tag="P5573_03910"
     CDS             2231..3205
                     /locus_tag="P5573_03910"
                     /inference="COORDINATES: protein motif:HMM:NF040786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism-associated LysR family
                     transcriptional regulator"
                     /protein_id="HRT27240.1"
                     /translation="MKYDGFKNITFQQMEALVHLVEERSFSRAAKKMLLTQPALTKHI
                     KNIEEVLGAKVVNRGGAGLSLTPEGNILHDYAGRILRLRDEVKEKIENARGNAGGVVT
                     VAASTIPATYILPRILKGFQEVCPGIQVRIESMDSEKALDSILNGEADIGFVGKSPPG
                     RRIYAEVFWPDRLILVVPAGHPWAKRKSVSAKKLVKEPFVTRERGSATREVVESCLKQ
                     NGGLNPADLNVAAELGSSEAVKEAVVAGVGASVISIHAVAREIRQGLLSGLTVKGCPF
                     ARSFYLIYRRRLDLMQYHRLFIDYARRQPIPADVDLLQQRSFVTWPIK"
     gene            3190..4581
                     /gene="thrC"
                     /locus_tag="P5573_03915"
     CDS             3190..4581
                     /gene="thrC"
                     /locus_tag="P5573_03915"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HRT27241.1"
                     /translation="MANKIRYYSTNRNLDSVPGVVPFKETVSFREALLQGQAPDEGLF
                     MPDPVPTLNLDDIYALKGKPYIEAAMLVSRAFLEAEVSGDVLEKVVVDSYPFGVPLER
                     VMDRKYIMRLDRGPTASFKDFAARMMARLMSHFRVEGSRLNVLVATSGDTGSAVGEAF
                     KGVEGIDVYILYPRAEVSGRQKKQLDTIGGNVLAISVDGKFDDCQNLVKQAFSDRALE
                     GLNLTSANSINFGRILPQIVYYVYAYAQLAGPGEEIVFSVPSGNFGNALGCEYARRMG
                     LPVRKLVMPTNENDEFPRFLKTGRYTKLSPSKACISNAMNVGHPSNLARFFDLYGGTV
                     DRNGTVHRYPDIEEMKRRIFSVSVTDEETRQTIKRVYASYGVVLEPHGAVGWKGLEDY
                     LAARGDFPLCVCLETAHPAKFPDEIVELIGVAPELPSGMMDLDMRKGEPIELPADYEE
                     FRYFLWKNLKTRE"
     gene            complement(4609..>5360)
                     /locus_tag="P5573_03920"
     CDS             complement(4609..>5360)
                     /locus_tag="P5573_03920"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: protein motif:HMM:NF003742.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HRT27242.1"
                     /translation="HFEHIKFDPVTVPFVDTVEKADETAAQIDADARDKGLPPIIFST
                     LLNKEVRTIIMKSEGVVFDLYDAFMGRLEDALHMRSTSGISRAHGLLDDSMYNIRIDA
                     VNYALRNDDGVSTKDYDKADVILIGVSRTGKTPTCLYLGLQFGIYAANYPLTEEDLYI
                     EYKKVPGVLQPYRDKLFGLTINPERLQKIRSERMPDSRYSSFVQCQRELAMAEYLFVS
                     KNIPFLNITSMSVEEIAATIMHKSRLPRRAL"
BASE COUNT         1195 a         1426 c         1612 g         1127 t
ORIGIN      
        1 ccttccgccg cgcccacgag gagcgcccgg ggtgcctccg ttcccagata ggctgtcacc
       61 ttgcaggcgg cgccgaccag ggccgccgtt ccctgcgtgc aggagagggc tttcccgtcg
      121 agaaacaacg actcttcgtc gaaagcgact ggtccgatgg tcaaggggaa atcctcatcc
      181 ggtatggtcc cgcagatgag cagcatatca acttctccct ccgttacctt tccagatgtc
      241 cagagactcg ataaaggccc tgtcgagcat gagagaacac agggtatagc cgtattcctt
      301 cggccgccgg gcttccgtca actttttgtc gatgagacag gcgtgcaggt aagggatgtc
      361 ggggcagccg cctcccgaga cgttcacgac cctgccggat tctttttcga aggtaagttt
      421 catgtttcct gccttgacca tcacggcctc gccgaaatcg gttaccttga cgacgccaag
      481 cagagcgcct ttgcctttca cgggtagtat ttccagatag ccggccttct tttcggcaaa
      541 caaccgctcg atacccgtct gctcgactaa gttgatctcc acggccaggt cgcaaccctt
      601 gcggagggcg ggcggcggcg cgacgagcct ggcctcgtaa ccggccgcca tcaagagcct
      661 ttctgctgcg atggcctctt cgacattgtt gaagaggatt atgccgcctt ccgactgccc
      721 tttctttttg aaatcaaaga ccattaaaca cccctatcga tcctgagcag gaacgtgtcg
      781 ccatgcgttt cacatgtgac ggtacagccg gaatggaccg ccatcctcgt cacattttct
      841 tttgccgttt gcgagtctgc ggttacttct atcggaaaac tgcccctgtc gatggccctc
      901 tttacaagga tgacgggctc cgggcaggaa agaccgcgtg tgtcgatcgt ctcggacatg
      961 gaatacctcc tattttaccg aatccgctct ctcatggaga aaccgatagc aagggtgatg
     1021 acaaggccga tgaggacggc cgattgaccc catgcggaag gaccgccgat cacgagaagc
     1081 tgcgccgccc tgtcaaactt gtccggggcg gccgcaaaat cgagattgtg ggcgaaggcc
     1141 gctcccatca gcattccgaa gatgaaaaca gtcgcgtcgg cgtctccttc gccggacatg
     1201 aagaattgtc gtcccgggca gccgccggca agggcgaaac acaagcccga taagcacatc
     1261 ccgaggaaat tccaaaggtg attagaatgt gcaatgggtt gctgtgcgaa accgatgttg
     1321 accttcccga tcagaaagtt gacggcgaag gcggccacaa cgaatgcaat gaccccgctc
     1381 aataagtgaa aatcttttat caggatgacg tcgcgaaggg cacccatcgt gcagaatctt
     1441 gtcctttggg caaggactcc tacgagcaat ccggcgccga gggaaaggaa gagtggcgcg
     1501 tgcatggagc cggggcctgc actactgaaa aagacaggcc cgtcggacgt gaatttgact
     1561 tcgagagcga cgagaaggaa aagaaaaagc atgaaggcag gcatgaccaa tcccacagcg
     1621 gtacggttcg gtgccgagcg gccgaggctg taaccggttt taagaaacca tacgccgacg
     1681 gtgattcccg ccgcaagacc cgcgataccg acgacggcgt tcaggtcccc gcctgccagc
     1741 cgggcaaggg cgcgccaggg acaaccgagg aacgcgagtg cccccaccat ggcgaagaag
     1801 ccgaggacga aacggatcag cggtgccgac cctcccctgg gcctgaattc ccggaaggcg
     1861 agggctgaca atagagaacc gaagacgaaa ccgaccagct cgggacggat gtactggacg
     1921 gcgccggccc ggtgaaggcc gatcgcgccc gccgtgtcac gggtgaaaca ggccacacag
     1981 acgcccatgt tcggtgggtt gcctagatag acgagataag ccgcgatcgc ccctatgatt
     2041 cctcccgccg tgacgattcc tgtgctggat gacagaaaat ctttcatcgc cttgccacct
     2101 cttttgatcg agatattttt ggcaaaattt gcttcgcacg ctttctatgt tgaataaaag
     2161 gaatatgtca aatcaataaa agttatcgac tgcgtttgtg tatagccggg acttatggta
     2221 tgaatcggcc gtgaaatacg acggattcaa gaacatcacc ttccagcaga tggaggccct
     2281 cgttcacctg gtcgaggagc ggagcttcag ccgggcggcg aagaagatgc tcctcaccca
     2341 gccggctttg acgaagcaca tcaaaaacat cgaggaggtg ctgggcgcga aggtcgtaaa
     2401 ccggggtggg gcggggctct ccctgacgcc ggaaggaaat atcctgcatg attatgcggg
     2461 gcggatcctc aggttgagag atgaggtgaa ggaaaagata gaaaatgcga ggggaaacgc
     2521 aggcggtgtt gtgactgtgg ctgcaagcac tatcccggcg acgtacatac tgccccgcat
     2581 cctgaagggt ttccaggagg tttgtcccgg tatccaggtg cgcatcgagt cgatggacag
     2641 cgaaaaggcc ctcgacagca tcttgaacgg cgaagcggac atagggttcg taggaaaatc
     2701 gccgcccggc cggaggatat acgccgaggt cttctggccc gaccggttga ttctcgtagt
     2761 ccccgccgga catccctggg caaagagaaa atcggtttcg gcgaagaaac ttgtgaagga
     2821 accttttgtg accagagaaa ggggttccgc aacgagagaa gtcgtggagt cctgtttgaa
     2881 gcaaaatggt gggttgaatc ctgcagactt aaacgttgcc gccgaactcg gcagctcgga
     2941 ggcggtgaag gaggcggtgg tggcgggcgt cggggcatcc gtcatctcga tccatgcggt
     3001 cgcaagggag atacggcagg gtctcctatc cggtctaacc gtcaaagggt gtccgttcgc
     3061 aaggtctttt tatttgattt acaggcgccg attggattta atgcagtatc accggttgtt
     3121 tatcgactac gcaaggcggc aaccgatacc ggcggacgta gatcttctac agcagaggag
     3181 ttttgtgaca tggccaataa aataaggtat tacagcacaa acaggaacct cgactccgtt
     3241 cccggggtcg ttccattcaa agagacggtt tctttccggg aggcgctgct tcaaggtcaa
     3301 gcgccggacg agggactttt catgcccgac cccgtcccga cgctcaacct ggacgacatt
     3361 tatgccttga agggaaagcc gtacattgag gctgccatgc tcgtttcgag ggcttttctc
     3421 gaggcagagg tgtccggtga tgttctggag aaggtcgttg tcgattcata ccctttcggg
     3481 gtgccgcttg agcgcgtcat ggacaggaag tacatcatgc gattggaccg ggggccgacc
     3541 gcttctttta aagatttcgc cgcccggatg atggcccgtc tgatgagtca ctttcgggtc
     3601 gaggggagca gattgaacgt ccttgtggcg acttccggtg atacggggag cgccgtgggc
     3661 gaggccttca aaggggtgga agggatcgat gtctacattc tttatccccg ggcggaggta
     3721 agcggaaggc agaaaaagca gctggacacc atcggcggca atgtccttgc gatctccgtt
     3781 gacggaaagt ttgacgattg ccaaaacctg gtaaaacagg ccttttctga ccgcgccctt
     3841 gaaggtctga acttgacctc ggcaaattcg ataaacttcg gaaggatcct tccccagatt
     3901 gtctattatg tctatgcata cgcccagctt gcggggccgg gggaggaaat cgtcttttcc
     3961 gtgccgtcgg ggaatttcgg caacgcgctc gggtgcgaat acgcccgccg tatgggacta
     4021 cccgtcagga aactcgtcat gcccactaac gagaacgacg aattcccccg ctttctcaag
     4081 acaggccgct acacgaaatt gtctccttcg aaagcttgca tctcgaatgc catgaacgtg
     4141 ggtcatccga gcaacctcgc acggttcttc gacctgtacg gcggcacggt cgaccgcaac
     4201 gggacggtac accgataccc cgacatcgag gagatgaaac ggcggatatt ttccgtgagc
     4261 gtgaccgatg aagagacgag gcagacgatc aagcgggttt atgcgagcta cggggtggtg
     4321 cttgaacctc atggtgcggt cggttggaag ggactggaag attacctcgc cgcccgcggt
     4381 gattttccgc tctgcgtttg tctcgagacg gcccatccgg ccaaatttcc cgacgaaata
     4441 gtcgaactta tcggggtcgc accggagttg ccgtccggta tgatggacct agacatgcgg
     4501 aaaggtgagc cgatcgagct cccggcggat tatgaggaat tcagatattt tctgtggaaa
     4561 aacttgaaga cccgtgaatg aggcctttgc aacggggctc gcgaatgttc atagcgcccg
     4621 ccgcggaagc ctcgatttgt gcatgatcgt cgcggcaatt tcctccaccg acatgctcgt
     4681 tatgttgagg aaggggatat tcttcgagac gaaaagatac tcggccatgg ccagctcccg
     4741 ttgacattgg acaaaagagg aatagcggct gtcgggcata cgctcggagc ggattttttg
     4801 cagtcgttcg gggttgatgg tgagtccgaa gagtttgtcg cggtaaggtt gcaggacgcc
     4861 ggggaccttc ttgtattcga tgtagaggtc ttcttcggtg agcgggtagt ttgccgcgta
     4921 gatgccgaat tgaagaccga ggtaaagaca ggtgggggtt ttccccgtgc gcgacacgcc
     4981 gatgaggatc acgtcggcct tgtcgtagtc tttcgtcgaa acgccgtcgt cgttacggag
     5041 ggcgtaattg actgcgtcga tgcggatgtt gtacatgctg tcgtcgagca gcccatgggc
     5101 gcgactgatt ccggaggtcg accgcatgtg gagcgcatcc tcgaggcggc ccatgaaggc
     5161 gtcgtaaagg tcgaacacaa ctccctcact cttcatgatg atcgttcgga cttccttgtt
     5221 gaggagtgta ctgaagatga tgggcggcaa ccccttatcc cgggcgtcgg cgtcgatctg
     5281 cgcggccgtt tcgtccgctt tttcaacggt gtccacgaaa gggacggtaa ccgggtcgaa
     5341 cttgatgtgc tcgaagtgaa
//
LOCUS       DAOTMX010000098         5268 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_13094_length_5268_cov_2.992902, whole genome shotgun sequence.
ACCESSION   DAOTMX010000098 DAOTMX010000000
VERSION     DAOTMX010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5268
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13094_length_5268_cov_2.992902"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="P5573_03925"
     CDS             <1..632
                     /locus_tag="P5573_03925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="integron integrase"
                     /protein_id="HRT27243.1"
                     /translation="VQRLLGFMEGKPALMAKLIYGSGMRLMECIRLRVQDIDFGQNLI
                     FVRGGKGGKDRTTILPRNLREEMFRQIAAVKSLHHRDLEEGFGEVYIPEALARKYPKA
                     SKQTGWQWVFPAKERSRDPRSGKVMRHHVLESVLQKAVKRAAHKAGIDKKVGCHTLRH
                     SFATHMLENGVNIRVLQELLGHADVKTTEIYTHVMSRDIRSLQSPLDRI"
     gene            852..2423
                     /locus_tag="P5573_03930"
     CDS             852..2423
                     /locus_tag="P5573_03930"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRT27244.1"
                     /translation="MKTKKTADNAAIDFNPQFQRALDIMEKTCKNVFITGRAGTGKST
                     LLNYFCDQTAKKAVILAPTGVAAVNVGGQTIHSFFGFKPDVTPSSIRRKVKDEKKNLY
                     KKLETIVIDEISMARADLLDCVGRFLRLNGPTEKLPFGGIQMIFIGDLYQLPPVVSGQ
                     EKEIFRGHYTTPYFFGAKVFDELDMEFVELEKIYRQKDGEFIRLLNAIRNRSVTDDDL
                     ALLNRRVDPEFSAPPDDFFICLTSTNEQANRINDEQIARLKGKTYTFHGLVEGDFGKE
                     YLPTQVDLKLKKGAQIMLLNNDTYGRWVNGTVGKMTGVKKDEDEGEVIIARLDNGMRV
                     EIAPYTWELFRLALKDGELTSEVVGSFTQYPVRLAFAVTIHKSQGKTFERAVIDVGRG
                     TFAHGQMYVALSRCTTLDGIVLKKPIRKSHILMDWQVVKFLTRFQYHQAERNLPREEK
                     IRLLREAIEKKQSMEIVYLKSKDEKSRRVIKPLVIGEMEYSGHLFFGLEAVCMTRRAK
                     RVFNVDKILEIAEVE"
     gene            2425..3333
                     /locus_tag="P5573_03935"
     CDS             2425..3333
                     /locus_tag="P5573_03935"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRT27245.1"
                     /translation="MEQNVTTVIDPEKCIGCGLCVVVCPLDTITMADGKAAVTGERSL
                     SCGHCAAACPTGAVRVGAVSDEALAFRTFRMSRDWLPYGDFDTAGLARLMASRRSCRN
                     YSDKPVDRALLEDLVGIGITAPSGTNCQYWTFTLLPERRHVRALTEEVARFFRKVNKM
                     AENGLLRGLLKTVGHRALDDYYRKYHAVVNQALDDWEKGGKDSLFYGAPAVIVIGSKP
                     GASCPAEDALLAAQNILLGAHSMGLGTCLIGFAVEAMRRDAAVKRSIGIPDEEKVYAV
                     VALGWPDEKYVRVAGRKEPLIRYCEP"
     gene            3591..4763
                     /locus_tag="P5573_03940"
     CDS             3591..4763
                     /locus_tag="P5573_03940"
                     /inference="COORDINATES: protein motif:HMM:NF037527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27246.1"
                     /translation="MEITPRTEILTKNELLNLIQKQKQPCISIYMPTHRVGADTQQNQ
                     IRFKNLLREAQEKTLAMGVRPGGEVETLFAPAFELHRNSLFWKKQSDGLAVFLAPGVF
                     RHYRLPLDFKESITVGERFYVKPVLPLFSSSGRFYVLALSQAEVRVLQGFRDHIMEVD
                     TEPLGMPKNLDQALRYDTPERETRAQPVAAGIGGGGMFHGHGGWTDYPKDNIMRFLLQ
                     VDRSLQKLLREEREPLVFAGVEYIFAMYREVTSYPYLMEEFIPGNPETVSSQELGKRA
                     WEISQRHHEKSLKEAAEKYRQSVGTGLASDNLLEIVPAAHHGRVGVLFVVKGEEKWGS
                     FNPGTGELYLHDRPERGDEDLLDLAALQTFLNRGDIFVVEKDRMPEGKPVAALFRY"
     gene            4894..>5268
                     /locus_tag="P5573_03945"
     CDS             4894..>5268
                     /locus_tag="P5573_03945"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRT27247.1"
                     /translation="MKDQSLDLTKGSLWQNIWQLSWPMFLMMIFNFFVGFTDVYVAGF
                     IGPEVQAAVGFVGILYFLVIIIANAISIGTLALVSRSIGAGDFRNAVEVGRQSLIFSV
                     IIAVILTGSGLLFYREIVLLAGI"
BASE COUNT         1250 a         1500 c         1464 g         1054 t
ORIGIN      
        1 aggttcaacg tcttcttggt tttatggagg gcaagcctgc gctgatggca aagttgatat
       61 acggaagcgg aatgagactc atggaatgta tccgcctgcg cgtccaggac atagatttcg
      121 gtcagaacct tatttttgtc aggggcggaa agggtggaaa ggacaggacg accattttac
      181 ccaggaatct acgtgaagaa atgttcagac aaatcgccgc ggtgaagtct ctacaccatc
      241 gggatcttga ggaaggcttc ggcgaggtct acattccgga agcgcttgcc agaaagtatc
      301 ctaaagcctc aaagcaaacc ggatggcaat gggttttccc tgccaaggaa cggtcgcgcg
      361 atccgcgctc cggtaaagtc atgcgtcacc acgttcttga atccgtcttg caaaaagcgg
      421 tgaaaagggc cgcgcacaag gccggaatcg ataaaaaagt cggttgccac acactgcgcc
      481 atagttttgc cacgcacatg ctggaaaacg gggtcaatat ccgcgtcctt caagaattgc
      541 tcggccacgc agatgtcaag accacggaaa tctataccca cgttatgtcc cgcgatattc
      601 gcagcttgca aagtcccctc gaccgcattt gacaagagcc gataagtttc catgtatcgc
      661 attgatccgg tcatcggccg acaatgcgcg agaaaacaaa cagaccccgg aatgcgctta
      721 aggtgctcat gtcgtggagc taagcggcga caatggggaa acaccgctta aaagggccga
      781 ttacctgttt gtcttgactt aaaccggata ttctgcgacg aatcagacag tcaacgaggg
      841 cctgctctgc gatgaaaacg aaaaaaaccg ccgataacgc cgccatcgac ttcaatcccc
      901 agttccagcg cgccctggat atcatggaga aaacgtgcaa gaacgtcttc atcacgggac
      961 gggcggggac ggggaaatcg accctcctca actatttctg tgaccaaacg gccaagaaag
     1021 ccgtcatcct cgccccgacg ggtgtggccg ccgtcaacgt gggcgggcag actatacatt
     1081 ccttcttcgg tttcaaaccc gacgtcactc cctcgtcgat ccgccggaag gtcaaggacg
     1141 agaaaaagaa tctctacaaa aagctggaga cgatcgtcat cgacgaaatc tctatggcgc
     1201 gggccgacct gctcgattgt gtgggcagat tccttcgcct caacggcccg acagaaaaac
     1261 tccccttcgg cgggatccag atgatcttca tcggcgacct ctaccagctt ccgcccgtag
     1321 tgtccggtca ggaaaaggaa atcttccgag gccattacac gacgccctat tttttcggtg
     1381 ccaaggtctt cgatgagctg gacatggaat tcgtggaact ggaaaagatc taccgccaga
     1441 aagacggtga attcatccgc ctcctgaacg ccatccgtaa ccgctcagtg accgatgacg
     1501 acctcgcgtt gctgaatcgg cgtgtcgacc ccgagttttc cgcgccgccg gacgacttct
     1561 tcatctgcct caccagcacc aatgaacagg cgaacaggat caacgacgag cagatcgccc
     1621 ggttgaaggg caagacttac acgtttcacg ggcttgtcga gggggatttc ggcaaggaat
     1681 acctgcccac gcaggtggac ttgaagctca aaaagggcgc acagatcatg cttctcaaca
     1741 acgataccta tgggcgatgg gtgaacggga ccgtggggaa gatgacgggc gtcaaaaaag
     1801 atgaagatga gggggaagtc atcattgcga ggctggataa cggcatgcgg gtggaaatag
     1861 cgccctacac atgggagtta ttccgcttgg ccctcaagga cggagaactg acctccgagg
     1921 tagtcggttc gtttacacaa tatcccgtcc ggcttgcctt tgccgttacc atccacaaga
     1981 gccagggcaa gaccttcgag agggccgtta tcgacgtggg aagaggcacc ttcgcccatg
     2041 gccagatgta cgtggccctc tcccggtgca cgacactgga cggcattgtg ctaaagaagc
     2101 cgatacggaa aagccacatt ctgatggact ggcaggtggt gaagtttctt acccggtttc
     2161 agtatcatca agcggagcgc aatctcccga gggaagaaaa gatacggttg ctcagggagg
     2221 ccatcgaaaa gaaacagagc atggaaattg tgtaccttaa atccaaggac gagaaatcac
     2281 gccgggtcat caaacccctt gtcatcggtg agatggaata tagcggccat ctcttcttcg
     2341 gcctggaagc cgtctgcatg acgcgccggg cgaaaagggt cttcaatgtg gacaagatcc
     2401 tcgaaatcgc ggaggtagaa tagtatggag cagaatgtca ccacagtcat cgacccggaa
     2461 aaatgcatcg gctgcggcct ctgcgtcgtt gtgtgtccgc tcgacactat taccatggcc
     2521 gacggcaagg ccgccgttac gggcgagagg tccctttcct gcgggcactg tgcagccgcc
     2581 tgccccaccg gggccgtccg cgtcggggcg gtgagcgacg aggccctcgc cttcaggacg
     2641 ttccggatgt ccagggattg gctgccttac ggagacttcg atacggccgg gcttgcgcgt
     2701 ctcatggcgt cgcgccgctc atgccggaat tacagcgaca aacccgtgga ccgcgccctt
     2761 ctggaagacc tcgtcggaat cggcatcacg gcgccgtcgg gcacaaactg tcagtactgg
     2821 accttcacgc tcctgccgga gaggaggcat gtccgcgcct tgactgaaga agtggcccgg
     2881 tttttccgca aagtgaataa gatggccgag aacggactcc ttcgcgggct cctgaagacg
     2941 gtcggacaca gggccctcga cgattactac cgcaaatatc acgccgtggt gaatcaggcg
     3001 ctcgacgact gggaaaaagg agggaaggac agtcttttct acggagcgcc cgccgtcatt
     3061 gtcatcggct caaagcccgg cgcgtcgtgc ccggcggagg acgccctcct tgccgctcag
     3121 aatattctcc tcggcgccca cagcatgggt ctcggcacct gcctcatcgg atttgcggta
     3181 gaagccatga gacgcgacgc agccgtcaag cgttcaatcg gcattcccga cgaggaaaag
     3241 gtctatgccg tcgtcgccct cgggtggccc gacgaaaagt acgtgcgggt ggcaggcaga
     3301 aaagagcccc tcatccgcta ctgtgaacct tgaaaggata gaaaggtgca aaatcaccac
     3361 attttgtaat gcattcaatc gttctcactt ttttttggta ccacgaactt gttgctattt
     3421 caatatcgat gttcgaatct cctcaataag agaaattgac aatgcaccat gatttcgcta
     3481 ctctcacatc atggtcatcc atcatggcga gcttctttgc aggctcaaga caggctctgt
     3541 caaccacgcc ttcccctctt tcacagacaa acaagaaaag gagcgaaaac atggagataa
     3601 cgcctcgcac tgaaatcctg acgaagaacg aactccttaa cctgatccaa aaacagaagc
     3661 aaccctgcat ttccatctac atgcctacgc accgggtcgg ggcggacacg cagcaaaacc
     3721 agatacgctt caagaatcta cttcgtgagg cccaggaaaa aaccctggcg atgggagtgc
     3781 gccccggcgg cgaagtggag acgctcttcg ccccggcctt cgagctccat cggaacagtc
     3841 tcttctggaa aaaacagagc gacggccttg ccgtctttct cgcccccggg gttttccggc
     3901 actaccggct gcccctcgac ttcaaagaat cgatcaccgt cggtgagagg ttttatgtca
     3961 agcccgtgct gccgctcttc agcagcagtg gacgatttta tgtcctcgcc ctgagccagg
     4021 ccgaggtcag agtccttcag ggattccgtg atcacatcat ggaggtcgac accgagcccc
     4081 tgggaatgcc gaagaacctc gatcaagcgt tgaggtacga caccccggag agggagacga
     4141 gggcgcaacc ggtcgcagcc gggatcggag ggggcgggat gttccacggt cacggcggct
     4201 ggaccgatta tcccaaggac aacatcatgc gcttcctcct tcaggtggac cgaagcctgc
     4261 aaaaactcct ccgggaagag cgcgaaccgc ttgtctttgc gggcgtggaa tacatcttcg
     4321 cgatgtaccg tgaagtgacc tcttaccctt acctgatgga agagttcatc ccgggaaacc
     4381 ccgaaacggt aagttcgcag gaactgggca aacgagcatg ggaaatctcc caacgtcacc
     4441 acgagaagtc cctcaaagaa gcggcggaaa agtacaggca atcggtgggc accggcctcg
     4501 cttccgacaa ccttctcgag atcgtccccg ccgcccatca cggccgggtg ggtgttctct
     4561 tcgttgtgaa aggagaggaa aagtgggggt cattcaaccc cgggacgggg gagctctatc
     4621 tgcacgacag gcctgaacga ggggatgaag accttcttga ccttgccgct ctccagacct
     4681 tcctgaacag gggcgacatc ttcgtcgtcg agaaagaccg gatgcccgaa ggcaaacccg
     4741 tcgccgccct tttccggtac tgatgcggga gataagcctt atttcttcaa gcagttgcag
     4801 aacggcggcg gttccccgtc gtcctgcaac cggccggtcc gtcaacataa acggcctgcc
     4861 gtggagggtc tggaataagg acaggctctc accgtgaaag accaatccct cgacctcaca
     4921 aaaggaagtc tctggcagaa catctggcag ctttcctggc ctatgttcct gatgatgatc
     4981 ttcaactttt tcgtggggtt caccgacgtg tacgtggcgg gcttcatcgg cccggaggtg
     5041 caggccgccg tcgggttcgt cgggatcctc tacttcctcg tcatcatcat cgccaatgcg
     5101 atcagtatcg gcacgcttgc cctggtctcg cggtccatag gtgcaggcga tttcaggaac
     5161 gccgtggaag tcgggaggca gtccctcatc ttcagcgtca tcatcgccgt catcctcacc
     5221 ggctcaggcc ttctcttcta ccgcgagatc gtccttctgg cgggaatc
//
LOCUS       DAOTMX010000099         5205 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_13285_length_5205_cov_3.331845, whole genome shotgun sequence.
ACCESSION   DAOTMX010000099 DAOTMX010000000
VERSION     DAOTMX010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5205
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13285_length_5205_cov_3.331845"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            311..679
                     /locus_tag="P5573_03950"
     CDS             311..679
                     /locus_tag="P5573_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27248.1"
                     /translation="MTIKKTVVSSVFVLLMLFVTGGSLYAAECSTVCKDGPPEYRGWS
                     TWTCDFSTGETRNLSVYAEPNPAKFSCALRKKGRFKNIDVSYGRYLNSKECLEDFCVP
                     ALIKICGVEDRCAELKNWIK"
     gene            735..1310
                     /locus_tag="P5573_03955"
     CDS             735..1310
                     /locus_tag="P5573_03955"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HRT27249.1"
                     /translation="MKKSLGGKTIIYPAPVLVVGTYDKEGRPNAMTAAWGGICCSSPP
                     CVAVALRKATYTYGNIVAQGGFTINIPSEKHVKYADYFGIVSGKKDDKFAATGLTPVK
                     SNIVNAPYIEEFPFALECRLIQKVELGLHTQFIGEILDVKADEDVLDGQGQLDVEKVK
                     PILYAPEANRYYGLGAFLGKAFSVGRDVKEK"
     gene            1335..2279
                     /locus_tag="P5573_03960"
     CDS             1335..2279
                     /locus_tag="P5573_03960"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HRT27250.1"
                     /translation="MKILFAAHEDAWGGVLGMLRKELPEHEFVPTGRFGFDSLRGFDV
                     LIPTMTVVSKAHLVEADRLRLIQQSGVGLEGVDIEAAKSLNIRVANVPADISGNADSV
                     AELGIYLMLGLSRNVRQIPESFANGLMGYPRGRALRGKTVGIIGLGGIGRALAKRLKP
                     FGVRLVGIKRQDPQKAKEELGLEWTGTPAELDELLNMSDYVVLCLPVTDESRNMLNRR
                     SFRAMKRDAFLINLSRGGLVDRDALEEALSTGTIAGAGLDVFWKEPVDPNDPIFKHNV
                     LATPHIGGATDVSIQGIMREVAENIRRLDRGQEPLYQM"
     gene            complement(2370..2445)
                     /locus_tag="P5573_03965"
     tRNA            complement(2370..2445)
                     /locus_tag="P5573_03965"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2410..2412),aa:Lys,seq:ttt)
     gene            complement(2503..3801)
                     /locus_tag="P5573_03970"
     CDS             complement(2503..3801)
                     /locus_tag="P5573_03970"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041584774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HRT27251.1"
                     /translation="MNSVVVVGTQWGDEGKGKIVDLYAEDADVIVRFQGGNNAGHTLV
                     VRGEQTILHLIPSGILHNRKVCIIGNGVVVDPAVLIGEIDNLTKRNLFPPDTKLYVSE
                     RAHIIMPYHKRLDAARENRNGAGKIGTTGRGIGPAYEDKVSRAGIRLCDLLDSDVFLD
                     RLRHNVEEKNFFLTKLFGEEPVDGDKIFSEYSAYAERLRPYAANTSVIINRAAKENKK
                     ILFEGAQGTHLDIDHGTYPFVTSSSTVAGSACCGSGVGPGCIRSVVGICKAYTTRVGG
                     GPFVTELADEVGTRLQQVGREFGATTGRKRRCGWVDMVQVRESIRLSGITGVAITKLD
                     VLTGIEKLKICVKYRSPQGEYTDSVPSNLKELSQCVPVFEEMDGWTEDMSKARRLQDM
                     PVKARKYLDRVEELFGVPIILVSVGADREETVVAQNPFSL"
     gene            complement(3822..>5205)
                     /gene="serA"
                     /locus_tag="P5573_03975"
     CDS             complement(3822..>5205)
                     /gene="serA"
                     /locus_tag="P5573_03975"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HRT27252.1"
                     /translation="VIGRAGIGLDNVDVIAASKKGIVVMNTPGGNTITTAEHTIAMMF
                     ALARKIPQATASMKAGKWEKNKFMGSEVYNKTLGIIGIGRVGSIVADRALGLKMNVIA
                     YDPFISHEAAEKLGITLVSLDELLQRSHFISVHTPMTKETRGMINASAFAKMRDGIFI
                     INCARGGIVSEKDLYDAVKSGKVAGAALDVFEEEPTKNTDLISLDRVICTPHLGASTE
                     EAQKNVAIAIAEQIVDYLTKGEIRNAVNFPSVTAEILSQIQPYLALAEKLGKFEAQIV
                     AGGIEEVHVEYSGEILNYNVAPVTISLLKGLLEPILNENINYINAPLVAKERGIKVIE
                     SRSSEVKDFTSLISLTVKTRKETSFIAGTIFGRQDPRIVRIGDFSMEVIPEGHMLVLY
                     NYDRPGVIGNIGTTLGNNNINIARFQLSREQVDGQALVVLSTDSVVPEEVLQKLLHLP
                     NVISVKQLEI"
BASE COUNT         1128 a         1347 c         1522 g         1208 t
ORIGIN      
        1 ggcgttcgcc ggaatgacga aacgcgtaat tctcgacttt ttacgagtcc atcatagatg
       61 agagtaaggt cgtcaaaaaa agaggtagga acatttcaac ctacccggcc tacctgcaat
      121 ccgatgagct cgggcaccac cagtcgggac cgtatcactg ctgtcgttga caatactata
      181 tctgacaaaa aaagaaaacg gtaatgagga atctggatat atcaaggcgg atatggtaaa
      241 aaaacccggc ggcatcagcg gtgtcgattc attttgcctg cttcggcagg tgccgatgga
      301 ggatggaaaa atgactataa agaagacagt tgtttcgagc gtgtttgtcc ttttgatgct
      361 tttcgtgacg ggcgggtcgc tttacgcagc ggagtgttcg acggtgtgca aggacggtcc
      421 gccggaatac aggggatggt cgacatggac ctgtgatttt tcaacaggag agacccgcaa
      481 tttaagcgta tatgccgaac cgaatccggc aaagttctct tgtgcgctgc ggaaaaaagg
      541 acggttcaag aacattgacg taagttacgg acggtacctg aactccaagg aatgccttga
      601 agacttctgt gtcccggcgt tgataaaaat atgcggcgta gaggatcgct gcgcagaact
      661 gaagaactgg atcaagtagt tccgacgttt tttcacttgg gcatccgagt ctattaacta
      721 accgggaggc gaaaatgaaa aaatcgctgg gcggaaagac gatcatctac cccgcccctg
      781 tgctggtggt ggggacctac gacaaggaag ggaggccgaa tgcgatgacg gctgcctggg
      841 gcggtatatg ctgctcgagt cctccgtgcg ttgccgtcgc gttgaggaaa gcgacttaca
      901 cctatggcaa catcgtcgct caaggaggtt ttacgatcaa catcccttcg gagaagcacg
      961 ttaaatatgc ggattatttc ggcatagtgt cgggtaagaa ggacgacaag tttgctgcca
     1021 ccggcctgac gccggtaaag agcaacatag tcaacgcccc gtacatcgaa gaattcccgt
     1081 tcgcgctgga atgccgtctg attcaaaagg ttgagcttgg gctgcatacc caattcatcg
     1141 gggaaatact ggacgtgaag gctgatgaag atgtccttga cggacaggga caattggacg
     1201 tcgagaaggt taaaccgatc ttgtacgccc ctgaagcgaa taggtattat ggtctcgggg
     1261 cgtttctggg caaggcgttt tccgtcggtc gggatgtgaa ggagaaatag gccagagact
     1321 tggagagaaa gagcatgaag atcctgtttg cggcgcatga agatgcttgg ggtggggtct
     1381 tgggaatgct caggaaagag ttgcctgagc atgaattcgt acccaccgga cgctttgggt
     1441 tcgacagctt gcgaggattt gacgttttga taccgacgat gaccgtcgtc agtaaagcgc
     1501 accttgtcga ggcggaccga ctccgtctta tccagcaaag cggggtcggc ctggaaggcg
     1561 tagatataga ggcggcaaag agcttgaata tccgggtggc gaatgtcccc gccgatattt
     1621 ccgggaacgc cgattctgtg gctgagttgg gtatatacct gatgctcggt ctgtcgagga
     1681 atgtccggca gattccggaa agttttgcga acgggcttat gggctatcct cgaggcaggg
     1741 ccttgcgcgg caagaccgtg ggaatcatcg gattgggagg catcggccgg gcgctggcaa
     1801 agaggctgaa acctttcggc gttcggttgg taggtatcaa gcgccaggat ccgcaaaagg
     1861 caaaagagga gcttggcctg gaatggacgg gaacgccagc cgagttggat gaattgttga
     1921 acatgtccga ctacgtcgtc ctctgcctac cggtgacgga tgagagcagg aatatgctga
     1981 acaggcggtc tttccgggct atgaagcggg atgccttttt gatcaatcta tccagaggcg
     2041 gtttggttga ccgtgatgcc ttggaagagg ccctctccac cgggacgatc gccggtgccg
     2101 gcctggatgt attctggaaa gagcccgtag atccgaacga cccgatattc aagcacaatg
     2161 ttctggcgac tcctcacata ggcggagcga cggatgtgtc catacagggg atcatgcggg
     2221 aggtggccga aaatattaga agactggacc gagggcaaga gccgctgtat cagatgtgat
     2281 gcctataccg cgatcacggc gcaggtgcga gtggagcaaa aaagcggtca acccgaaaga
     2341 gctaaccgct tgataccatg agcatttatt ggtgggccgt gcgagaatcg aactcgcgac
     2401 caacggatta aaagtccgct gctctaccgt ctgagctaac ggcccacaat tttaaaggtg
     2461 caacttttaa cagcacgctt cgttgctgtc aagaaggaac tgctaaagcg agaaggggtt
     2521 ctgagcgacg acggtttctt ccctgtccgc cccgactgat acgaggatga tgggtacgcc
     2581 gaatagttct tcgacacggt cgaggtactt tcgcgccttt accggcatgt cctgcaggcg
     2641 cctcgcctta ctcatgtctt ccgtccatcc atccatctcc tcgaagacgg gaacgcactg
     2701 ggaaagctcc ttcaggttgg aagggacaga atccgtgtac tccccctgag ggcttctgta
     2761 cttaacgcag attttcaact tttcaattcc ggtgaggacg tcgagctttg tgatggcgac
     2821 cccggtgatg ccggaaagcc ggatcgactc cctgacctga accatgtcga cccacccaca
     2881 acggcgcttc ctgcccgttg tggcgccgaa ctctcgtcct acctgctgaa gccgtgtacc
     2941 gacttcgtcg gcgagttccg tcacgaatgg acccccgcct acgcgggtcg tgtaggcctt
     3001 gcaaatgccc acaacggacc tgatgcatcc ggggccgacc cccgacccgc aacatgcgct
     3061 cccagcgacc gtgctcgatg aggtcacgaa aggataggtg ccgtgatcga tgtcgaggtg
     3121 cgttccctgt gcgccttcga agaggatctt cttgttttcc tttgccgccc tgttgatgat
     3181 gaccgatgtg ttagcggcat agggtctcag cctttccgcg taggcactgt attcggagaa
     3241 aatcttatcg ccgtcgacgg gttcttcgcc gaatagcttg gtaagaaaga aattcttctc
     3301 ttccacgttg tgtcggagtc ggtccaggaa aacgtcgctg tcgagtaagt cgcagagccg
     3361 tatacccgcg cgggatacct tgtcctcgta agccggaccg ataccccgac ccgtcgtccc
     3421 aatcttccca gccccattcc tgttttcccg ggcggcgtcg agacgtttgt ggtaaggcat
     3481 gatgatatga gccctttcgc tcacatacag tttcgtgtcc ggcggaaaga ggttcctctt
     3541 tgtcaggttg tcgatttctc cgatgagaac ggccgggtcg accacgacgc cgtttccgat
     3601 gatgcagacc ttacggttgt gcagtatccc ggaagggatc aggtgaagga tcgtctgctc
     3661 tcccctgact accagtgtgt gtccggcgtt gtttcctcct tgaaaacgga cgatcacgtc
     3721 ggcgtcttct gcgtagaggt ctacgatttt tcctttacct tcgtcgcccc actgggtccc
     3781 gacgacaact acactgttca tggcctctct ccccgaatac gtcagatttc aagttgcttg
     3841 acggagatta cgttcggcag gtgcaagagt ttttggagca cctcttcggg aactacgctg
     3901 tccgtgctca ggacaaccag ggcctggccg tcgacctgct cgcggctcaa ctgaaaccgc
     3961 gcgatgttga tgttgttgtt tcccagagtg gtcccgatgt tcccgatgac accgggcctg
     4021 tcgtaattgt acagaaccag catgtgacct tcagggatga cctccatgga gaagtcaccg
     4081 atcctcacga tccgcgggtc ctgcctgccg aagatggtac cggcgatgaa actcgtttcc
     4141 ttcctcgttt tcacggtcag cgagatcaga ctcgtgaaat ccttgacctc gctgctcctg
     4201 gattcgatga ccttgatgcc gcgctccttt gccaccagcg gggcgttgat gtagttaatg
     4261 ttctcgttga ggataggctc cagcagaccc ttgagaagag agatcgtgac tggcgcgacg
     4321 ttgtagttga ggatctcccc gctgtattcc acgtggactt cctcaatacc acccgccacg
     4381 atctgggcct cgaacttgcc gagcttctcg gcaagcgcca gatagggctg aatctgagaa
     4441 agaatttctg cggtgacgga ggggaaattc acggcattgc ggatctctcc tttggtcagg
     4501 tagtccacga tctgctcggc gatggcgatg gccacgttct tctgcgcctc ttccgtcgag
     4561 gccccgagat ggggcgtgca gatgacgcgg tcaagggaaa taagatccgt gtttttcgtc
     4621 ggttcttcct cgaagacgtc gagtgccgcg cccgccacct ttcccgactt gaccgcgtcg
     4681 taaaggtcct tttcgctgac gattccaccc cgtgcacagt tgatgataaa aatgccgtcc
     4741 ctcatcttgg cgaaggcgga ggcattgatc atgccccttg tttccttcgt cattggggtg
     4801 tggacggaaa taaagtggga tcgctgcaga agctcatcga gagaaacgag cgtgatgccg
     4861 agtttctccg ccgcctcgtg ggagatgaaa gggtcgtagg cgatgacgtt catcttcagg
     4921 ccgagggcac ggtcggcgac gatggaaccg acacggccga tgccgatgat gccgagggtc
     4981 ttgttgtaga cctcggagcc catgaatttg ttcttctccc attttccggc tttcatggaa
     5041 gccgtcgcct ggggtatctt ccgcgctagg gcgaacatca tggcgatggt gtgctcggcc
     5101 gttgtgatcg tgttcccgcc gggggtgttc atgaccacga tacctttctt gctcgcggcg
     5161 atgacgtcga cgttgtcgag gccgatcccg gcccgtccta tgact
//
LOCUS       DAOTMX010000100         5169 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_13377_length_5169_cov_4.398514, whole genome shotgun sequence.
ACCESSION   DAOTMX010000100 DAOTMX010000000
VERSION     DAOTMX010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5169
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13377_length_5169_cov_4.398514"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1811
                     /gene="leuS"
                     /locus_tag="P5573_03980"
     CDS             <1..1811
                     /gene="leuS"
                     /locus_tag="P5573_03980"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HRT27253.1"
                     /translation="FMLVAAEHPLVMKLAKGKPCEKAVLEFVEKVKKQDRMMRTSDYY
                     EKEGVFLDAYCLNPLTNQKMPIYAANFVLADYGTGCVMAVPTHDQRDFEFAKKYDLPL
                     IVVIQNPEMSLDPATMTEAYVGEGTLVNSGQFNGMDNLKALDAMADYLESIGRGKRTV
                     QYRLRDWGISRQRYWGAPIPIVYCDKCGTVPVPEKDLPVVLPLDVQITGEGESPLVRH
                     RAFVETTCPSCGGDARRETDTMDTFVESSWYFDRFCSPDYNEKPGLDRKRMDYWLPVD
                     QYIGGIEHAILHLLYSRFFTKVLRDFGVLGVDEPFANLLTQGMVCKETERCDVHGYLY
                     PSEVKDGKCVHCSREVKVGKAEKMSKSLKNVVDPDYLIEKYGADTARMFCLFTSPPEK
                     DLEWSDQGVEGSFRFLCRVWRIVTEYLDDVKDVEAFPNDELAGEIKDLRRKTHQTIKK
                     VTDDINDRFHINTAISAVMELVNAIYQVKRPDREDRKDLAVLKEALEAVVVLLAPIVP
                     HITEELWEMMGREKRLVDTPWPVYDEAVAAEEEITVVVQVNGKLRGKVVVPADESDER
                     IKELAVKDKKVGRFLEGKKPLKVIYVPKKLVNIVVN"
     gene            1957..2442
                     /locus_tag="P5573_03985"
     CDS             1957..2442
                     /locus_tag="P5573_03985"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HRT27254.1"
                     /translation="MVLMVGCGYRFSPGGEHIDEGIRTVYVDSFANRTSEVNVEVILR
                     NAFIERLRKSSRFRLADRREDADAILRGSVSNLSVTHLSYSTSDLAREDRVTLTMELV
                     FEEKKDRKVIWSNPSFSWYSDFAVVQADTLKTELNKKSALDKLAADLADRAYRMIMSG
                     F"
     gene            complement(2607..2870)
                     /gene="rpsT"
                     /locus_tag="P5573_03990"
     CDS             complement(2607..2870)
                     /gene="rpsT"
                     /locus_tag="P5573_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HRT27255.1"
                     /translation="MATHKSAEKRNRQSRKRRLRNMSAKSLVKTMTKKVLKAVGEKDQ
                     EGAKKRLLEAVVAIDKAASKRVFHKNTAARKISKLTRKVNSLA"
     gene            complement(2964..3701)
                     /locus_tag="P5573_03995"
     CDS             complement(2964..3701)
                     /locus_tag="P5573_03995"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRT27256.1"
                     /translation="MEGKTIRTTVEDRIGTITLSRPENRNAISIQMRREISACLDEWQ
                     CSEKVHAVVFTGSGAIFSAGFDLKEFGRPELFDELYSTSALYHREIWHFPKPVIAAVN
                     GAAVAGGLDLATMCDIRICDPSAVFGHPEIKFGVPPLFTPLRWIVGDGIARDLCLTGR
                     RINAEEALRIGLVSEVVRDESLIERAIRLAKEILEAPFPALQFVKGYLKDNANRGFEE
                     SFCVEHDKAFQEFLLKKAVEGLKKGSP"
     gene            complement(3739..4260)
                     /locus_tag="P5573_04000"
     CDS             complement(3739..4260)
                     /locus_tag="P5573_04000"
                     /inference="COORDINATES: protein motif:HMM:NF039354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6125 family protein"
                     /protein_id="HRT27257.1"
                     /translation="MDFSALEKMDRDELNSYLEFLLWHYRVIDGFWFLFVSDEFGQQC
                     AERINERVWAKAAELAAKDLLKRLKIEEKGLKGFVKAQKLFPWHIIMGYKIEEKDDEV
                     ILTVPVCAPQAARLRKGLPEFFCKAMHKGEFENFAKVVDDRIQVECLFAPPDPHPPEV
                     FCKWRFTLKKETP"
     gene            complement(4389..>5169)
                     /locus_tag="P5573_04005"
     CDS             complement(4389..>5169)
                     /locus_tag="P5573_04005"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HRT27258.1"
                     /translation="PQLAAALAFYTIFSLAPLLIFGTYAAGLIFGPEAGRGELIGQIQ
                     NLVGNEAAYLIKKLTAGKRFQAVAPATIIAFTAMFIGATAVFVELHGALNKIWNVKPA
                     AGVIVGIIKPRLLSFVMILGLGFLFLVSLLSTAVIGTVGSILKKFAPGVFYILTNLGT
                     GIISFIIITALFAMVFKLLTAAKISWRAVWVGAITTSVLFAIGKYLIGFYLTHSGTAS
                     IYEAAGSLVVFIFWVYYSAQVFYYGAEITYVYARHHEEADP"
BASE COUNT         1191 a         1357 c         1511 g         1110 t
ORIGIN      
        1 ccttcatgct ggtcgcggcg gaacatccgc tggtcatgaa actggcgaag gggaagccct
       61 gcgagaaagc ggtccttgaa tttgtggaaa aagtgaaaaa gcaagacagg atgatgcgca
      121 cctccgatta ttatgagaag gagggcgtgt tcctcgacgc ctactgcctg aatcccctca
      181 ccaaccagaa gatgccgatc tatgccgcca acttcgtcct tgccgattac ggcaccggct
      241 gcgtcatggc cgtgccgacc cacgatcagc gcgacttcga gtttgcgaag aagtatgacc
      301 tccccctcat cgtggtcatc cagaaccctg agatgagcct cgaccccgcg accatgacgg
      361 aggcctacgt gggggaaggg accctggtga actcggggca attcaacggc atggacaacc
      421 tgaaggccct cgatgccatg gcggattacc tcgaatctat cggacgcggc aagcggaccg
      481 tccagtatcg tctccgggac tggggtatat ctagacagcg ctactggggg gcaccgatcc
      541 ccatcgtcta ctgcgacaag tgcggcaccg ttccggtacc cgaaaaagac ctccccgtcg
      601 ttctacccct cgatgtgcaa atcacggggg aaggcgagtc tccattggtg cggcataggg
      661 cattcgtcga aacgacatgc ccgtcctgcg gcggggacgc gaggcgggag acggacacga
      721 tggacacatt cgtcgagtct tcatggtact ttgaccggtt ttgctccccc gattacaacg
      781 agaagcccgg gctcgaccgg aagcggatgg actactggct ccccgtcgac cagtatatcg
      841 gcggcatcga gcacgccatc cttcatcttc tttattcgag atttttcaca aaggtcctga
      901 gagacttcgg cgtcctggga gtcgatgaac cctttgcaaa cctcctgacc caggggatgg
      961 tctgtaaaga aaccgagcgc tgtgacgtcc atggttacct ctatccctcc gaagtaaaag
     1021 acggtaagtg cgtgcactgc tcccgggagg tgaaggtcgg gaaagcggag aagatgtcga
     1081 agtctttgaa gaatgtggtc gacccggact acttgatcga gaaatacggc gccgacacgg
     1141 cgaggatgtt ctgtcttttc acttcacccc ccgagaaaga cctggagtgg agtgatcagg
     1201 gcgtcgaagg ctccttccgt ttcctctgtc gggtgtggcg aatcgtcacg gagtacctcg
     1261 acgacgtcaa agatgtcgag gcctttccca acgatgagct cgccggcgag atcaaggact
     1321 tgagacggaa gacccaccag acaatcaaga aggttaccga tgatatcaac gaccggttcc
     1381 atatcaacac cgccatcagc gccgtgatgg aactggtcaa cgccatctat caggtgaagc
     1441 ggccggatag ggaagatcgc aaggaccttg cggttctcaa ggaggccctt gaagccgtcg
     1501 tcgtcctgct ggcaccgatc gttccccata tcaccgaaga gctgtgggag atgatgggcc
     1561 gcgaaaaaag gctcgtcgat accccttggc cggtgtatga cgaggccgtc gccgcggaag
     1621 aggaaatcac cgtggtcgtt caggtcaacg gtaagctccg gggaaaggtc gtcgttcccg
     1681 ccgatgaatc ggacgaacgg atcaaagagc tcgccgtgaa agacaagaag gtggggaggt
     1741 tcctggaggg aaagaagccg ctcaaggtta tctatgtccc caagaagctg gtcaacatag
     1801 tggtaaatta ggtatcgggc atcagacaca agggaaatgt ttaaaacgga gggaataggc
     1861 cggagggaaa gagtgcgaat gttcaagcgg agaaagccgg acggagtaag aatggggcaa
     1921 aatatatgtg cgtgggtcgc gacgtcatta ctcgcgatgg ttttgatggt tggttgcggc
     1981 taccgtttct cacctggcgg ggaacacatc gacgagggta tcaggacggt ttatgtggat
     2041 agtttcgcga accggacgag cgaggtcaat gtcgaggtca tcttacgaaa cgccttcatc
     2101 gagagattac gcaagagcag ccgcttcagg cttgccgaca ggcgggaaga tgccgatgcg
     2161 atcctgaggg gaagcgtcag caacctttcc gtgactcacc tttcgtactc gacgtcagac
     2221 ctggccaggg aagacagggt gacgttgaca atggagcttg tttttgagga gaagaaggac
     2281 aggaaggtca tctggtcgaa cccgtctttt tcctggtata gcgactttgc ggtcgttcag
     2341 gcggacactt taaaaacgga attgaacaaa aagagcgcct tggacaaact ggcggcagac
     2401 ttggcggaca gggcctacag aatgatcatg tcggggtttt agctgtaatc atcaacattg
     2461 tcggcgcgac gaatgcggca aagaacccga ctgtgcttcg ccggcacggg acagtaatgg
     2521 tagagaatag gccacacaat ctcggagcac gaaaagtaaa agcccctact ctgcaggggc
     2581 ttttactttt cggcctgaat agggcgttac gccaaagagt ttacctttct ggtcagcttg
     2641 gaaatcttgc gggcggccgt gttcttgtga aagaccctct tgctcgccgc cttgtcgatg
     2701 gcgacgacgg cttcaagaag cctcttcttc gctccttctt ggtccttctc cccgacggct
     2761 ttaagaacct tcttggtcat cgtcttgacg agcgacttgg cggacatgtt cctcaaccgc
     2821 cttttcctgc tctgccggtt tctcttctct gctgatttat gagtagccaa cggtctatct
     2881 cctcccggtg attttcaaag gccctttgtc tacaggagat ttctctttct gtcaatggga
     2941 aaaatcggca ggcaaaaaac aggtcatgga ctgccttttt tcagtccttc gacggccttt
     3001 ttgagcaaaa actcctggaa ggccttgtcg tgctcgacgc aaaaggactc ctcgaaaccg
     3061 cggttcgcat tgtccttgag atatcccttg acgaattgga gggctggaaa gggggcttcc
     3121 agtatttctt tcgcgagacg gatcgccctc tcaataaggg attcgtcccg gacgacctcg
     3181 ctcacaaggc cgatgcggag cgcctcttcg gcgttgatgc gccgtcccgt aaggcaaagg
     3241 tcgcgggcga tgccgtcgcc gactatccag cgcagaggcg tgaaaagtgg cggcacgccg
     3301 aacttgatct cagggtggcc gaagacggcg gaaggatcgc agatgcggat gtcgcacatc
     3361 gtggcgaggt cgagaccgcc agcaacggcc gcaccgttga cggctgctat caccggcttc
     3421 gggaaatgcc agatttcccg gtggtagagg gcggacgtgg aataaagttc atcgaacagc
     3481 tccggcctcc cgaattcctt gaggtcgaag ccggcgctga aaatcgcgcc cgagcccgtg
     3541 aagacgacgg catggacctt ttccgagcac tgccattcgt cgaggcacgc cgagatttcc
     3601 cgccgcatct ggatcgatat ggcattgcgg ttctccggtc ggctcaatgt tatcgttccg
     3661 atccggtcct cgactgtggt gcgtatcgtc ttgccttcca tgccgaccct tgccggtgcg
     3721 ggtaaagaaa gtaacagatt aaggcgtttc ctttttcagt gtgaatctcc acttgcagaa
     3781 gacttccggg ggatgggggt ccggaggggc aaagaggcat tccacctgaa tcctgtcgtc
     3841 caccaccttc gcgaagttct cgaattctcc tttatgcatg gccttacaga aaaactccgg
     3901 cagcccctta cgcaaccttg ccgcctgagg ggcacacacg ggcaccgtca ggataacttc
     3961 gtcgtctttc tcttctatct tgtagcccat gatgatgtgc cagggaaaaa gcttttgggc
     4021 cttgacgaag cctttcaggc ccttctcttc gatctttaag cgctttaaga ggtcctttgc
     4081 cgcgagctcc gcggccttcg cccagacccg ttcgttgatc ctttcggcgc actgttgacc
     4141 gaactcatcg gaaacgaaga ggaaccaaaa accgtctatc acccggtagt gccagagcaa
     4201 gaattcaaga tagctgttca attcatcccg gtccatcttt tcgagggctg aaaagtccat
     4261 ttcacctccc aaatgcgggt cttgaatgct gaaaagtgaa caacgccagt aattatgaaa
     4321 tgggtatact cgacaagtcg gagcggcttc attagagact ttttcaggtt cctttgcaag
     4381 gacaaaattt aaggatcggc ttcctcgtgg tgtcgggcat agacataggt gatctcagcg
     4441 ccgtaataga aaacctgggc cgagtagtag acccagaaga tgaagactac caaggagccc
     4501 gccgcctcgt agattgaggc cgtgccgctg tgagtcaggt agaagccgat gaggtatttg
     4561 ccgatggcga aaagtacaga cgtggtgatg gccccgaccc agaccgctcg ccaggatatt
     4621 tttgctgccg ttaggagttt gaagaccatg gcaaagagag cggtgatgat gataaacgag
     4681 attatgccgg tccccaggtt ggtgaggatg tagaatacac ccggcgcgaa ttttttcagg
     4741 atgctgccga ctgtcccgat gacagccgtc gagaggagcg agactaagaa gaggaagccc
     4801 aggccaagaa tcatgacgaa ggaaagaaga cgcggcttga tgatgccgac gatgacgcct
     4861 gcggcgggtt tgacgttcca tatcttgttc agggcgccgt gcaattcgac gaataccgcc
     4921 gtcgccccta tgaacatggc ggtgaaggcg atgatggtgg ccggtgcgac ggcttggaac
     4981 cttttcccgg cggtcaattt cttgatcaga taggccgctt cgtttcccac taggttctga
     5041 atctggccga tcaactcacc gcggcccgct tccggtccga agatcaggcc ggcggcgtaa
     5101 gtgccgaaga tgagcagggg ggcgagggaa aagattgtat aaaaggcaag ggcggcggca
     5161 agctggggt
//
LOCUS       DAOTMX010000101         5121 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_13529_length_5121_cov_4.496644, whole genome shotgun sequence.
ACCESSION   DAOTMX010000101 DAOTMX010000000
VERSION     DAOTMX010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5121
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13529_length_5121_cov_4.496644"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="P5573_04010"
     CDS             <1..636
                     /locus_tag="P5573_04010"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HRT27259.1"
                     /translation="LDLREAAGDADVVVEVVIEVMDVKKKVFAELEEIVKPDTFFFTN
                     TSGLSITEMAAFTKRPDKFIGTHFFNPVPVMRLLEIIKGYETSDETLEVAKGWGKKIG
                     KEVIVVNEAPAFAVNRVLCPMINEAFFALGEGVASAEDIDKGMVLGCNHPIGPLALAD
                     LVGLETLLRVMEGLHRELGDKYRPAPLLVKLVRAGRYGRKTGKGVYDYTKK"
     gene            689..2347
                     /locus_tag="P5573_04015"
     CDS             689..2347
                     /locus_tag="P5573_04015"
                     /inference="COORDINATES: protein motif:HMM:NF006182.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HRT27260.1"
                     /translation="MSGFLKPLTEEEKARYNVINKENIEFLMDRYNRVNRWVIADMIR
                     RSAYHYPDKKALIFQDSALTYAQLEEEINRTANALQDLGVKKYDRVAILAHNTTHHVL
                     TWLACCKIGAIYLAINYLLRGKDISYCINHSESVAFIVEDALYDLVKDVLDDMPTVKS
                     WVWSNQGAGKPCIGGKFVDFDEWYKKYPATEPEAILHIEDPCQMTYTSGTESLPKGVI
                     VSNQALLAEYMGCIVDGGYDEDDVSVNAMPIYHCAQRDVFLNPIFWVGGTNILIMPDI
                     GQILKTIAAHKATLFFAAPTVWIGILRHPDFSKYDLSSLKKCYYGASIMPVEVLKEMM
                     EKFPGARIWNYYGQTELAPYHTILKAKDAFTKIGSAGMGGLNMETRLEEGDHQPVTQP
                     GVPGEICGKGPHAMIMYFKDPEKTEEAFLCGWFHSGDVGVMDEDRYITVADRKKDMVK
                     TGGENVPTREVEEVIYLDKRVQEVAVIGLPHEKWVEAVTAVVVPRAGEKITEEELLEL
                     CKKELAPFKVPKGFIFLDALPKTPTGKILKREMRVTYKDFYKDK"
     gene            2436..3887
                     /locus_tag="P5573_04020"
     CDS             2436..3887
                     /locus_tag="P5573_04020"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRT27261.1"
                     /translation="MDILLVNPWHNGRAEIPPLGLQCLAGSLMNDGIEAVILDLDVWP
                     KEDVEGILEETLGNSRPRILGVGALSDSFTSAMETCVLAKSLNADILTVLGGMHATVL
                     DVQVLEEHPQVDIVVRGEGEKTFLELVRRCLDSGDCKGVDGISYRDGKDVVRNQDREL
                     EKNLDRFPLPERSAQDVSRYRTQSVSSSRGCRRNCSFCSIQSQYRHTVRVRSPESLMR
                     EIGELVSLGARRIMFTDDNFTFSLERVRGICKSIIERGFDRCVEFYAEGCLEDICKNP
                     LIAQILGQSGFRALYAGAESGSPKILARYGKNLSPVDVLKGASYCLEQNVMPVLNFIL
                     LGPWDTVDTIRQTIDLAKQSFENGAEIAYAEMLIPYPGTPVQEELVRDGKFRQRDNVF
                     YFESYEGISVEWFLRTCDLARDITRHTRGTERLFGMRKAYHELTCLQSLLEGDIPVGL
                     RDCSGVRETEYLERKIEDWFLLTKDLMEGIIPV"
     gene            3967..5109
                     /locus_tag="P5573_04025"
     CDS             3967..5109
                     /locus_tag="P5573_04025"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRT27262.1"
                     /translation="MDFDLTDEHKMLKDMAYKFAVNEIAPISLECDREERYTPEIRKK
                     AAEQGLVGAWVPEQYGGAGVGFLGNTIITEELSRVDMGIGLNVVAASFGCEAVFYFGT
                     EEQKHKYLTPVCRGEWVAAGAFTEPNAGTDVAGYKTRAVRDGSDFVINGNKMFITNGS
                     VCDFMVAQCITKPEEKKHNSFSQIIVPATAKGVTRNKLHGKLGIRSSDTAEITFEDVR
                     VPQENLVGKDGRGFHQLMHFFDVTRVMVSGQAIGLSQACLDQAIKYAKERTAFGAPIG
                     SFQATMAKLTEMAIKVESLRTLVYKAAWLVDVGRPDYHLAAMAKYLGGQTAVFCANAA
                     VEIHGGYGYMEEYSVQKWYRDAKILELYEGTKEAEIMTIGRVLQSK"
BASE COUNT         1297 a         1189 c         1554 g         1081 t
ORIGIN      
        1 ctcgacctga gagaggcggc gggtgatgct gacgttgtag ttgaagtagt gatagaagtc
       61 atggacgtga agaagaaggt ctttgcggag ttggaggaga tcgtaaagcc tgacacgttt
      121 tttttcacga acacctcggg gttgagtata acggagatgg ccgccttcac gaagcggccc
      181 gacaagttta tcgggaccca ttttttcaat cctgtgcctg tgatgcgttt acttgagatc
      241 ataaaggggt atgagacgtc ggacgagacg ttagaagtag cgaagggatg gggcaagaaa
      301 atcggtaaag aagtcatagt agtcaatgaa gctcccgcct ttgcggtgaa ccgagtttta
      361 tgtccgatga taaacgaggc ctttttcgcc cttggggagg gggtagccag tgcggaggac
      421 atcgacaagg gcatggtgct tgggtgtaac catcccatcg gtcccttggc gttagccgac
      481 ctggtaggat tggaaacgct attgcgggtc atggagggtt tgcacaggga gttgggtgac
      541 aagtaccggc cggcacctct cctcgtgaag ctggtgaggg caggccgtta cggcaggaag
      601 accggcaagg gcgtctacga ttacacaaag aagtagaagg gggagagaca gtaagctgac
      661 aaccttatta aaaaaggagg gagaacgaat gtcaggattt ctgaaaccgt taacagagga
      721 ggaaaaagca agatacaacg tcatcaacaa agaaaacatc gaattcctca tggaccgtta
      781 caacagggtg aaccgctggg tcatcgccga catgatccgg agaagcgcct atcattaccc
      841 agataagaag gcactcatct tccaggatag tgcgctcacg tacgcgcagt tagaggagga
      901 gatcaaccgt acggccaatg ccttgcagga cctgggcgtc aagaagtacg accgggtagc
      961 catcctggcg cataacacaa cccaccacgt gctgacgtgg ctagcctgct gtaagatcgg
     1021 ggcaatctac ctcgccatca actatttgtt gaggggtaaa gacatctctt actgcatcaa
     1081 ccactctgaa agcgtagcct tcatcgtcga agacgccttg tatgaccttg ttaaagacgt
     1141 tcttgatgat atgcccaccg tcaagagttg ggtctggtcg aatcaggggg caggaaagcc
     1201 ctgcatcggg ggaaaattcg tcgattttga tgaatggtac aaaaaatacc cggcgacgga
     1261 gccggaagcg atcctgcaca ttgaagaccc ctgccagatg acttacacaa gcgggacgga
     1321 atcgctcccg aagggtgtga tcgtcagcaa ccaggcgctg ttggcggagt acatgggatg
     1381 catcgtcgac ggcggttatg atgaggatga tgtatcggtg aatgccatgc cgatttatca
     1441 ttgtgcgcaa cgcgacgttt tcttgaatcc gatcttctgg gtcggaggca cgaatatcct
     1501 catcatgccg gatatagggc agattctcaa gactatcgcc gctcataagg cgaccctctt
     1561 cttcgcagcc ccgaccgtat ggatcggcat cctgaggcat cccgatttca gtaaatacga
     1621 cctctcaagc ctgaagaaat gttactacgg cgcctcgatc atgcccgtcg aggttttgaa
     1681 ggagatgatg gaaaagttcc cgggggcaag gatctggaat tattacggcc agacggagct
     1741 tgcgccctac cataccatcc tgaaagcaaa agacgccttc accaagatag gctcagcggg
     1801 tatgggcggg ttgaacatgg agacgcggct ggaggaaggc gaccatcagc cggttacaca
     1861 gccgggcgta cccggagaga tatgcggcaa agggccccac gccatgatca tgtatttcaa
     1921 ggacccggag aagaccgaag aggccttcct gtgcgggtgg ttccattccg gcgatgtcgg
     1981 ggtgatggat gaagaccgat acatcaccgt ggccgacagg aaaaaagaca tggtcaagac
     2041 cggcggtgag aacgttccga ctcgcgaggt agaggaggtc atttatctgg acaagcgggt
     2101 acaggaagtt gccgttatcg gcctgccgca cgaaaagtgg gtggaagcgg tcacagcggt
     2161 cgtggtccca cgggcaggcg agaagatcac cgaagaagag ctcctggaac tgtgtaagaa
     2221 agaattggcc cccttcaagg ttccgaaggg tttcatcttc ctcgacgccc ttcccaagac
     2281 gcctacggga aagatactca agcgtgagat gcgggtaact tataaagatt tctacaaaga
     2341 caaataagcg cttctcgagg aaaggcatcg tggataacgg atgcggtgcc tttcctgctt
     2401 tgattttttg atcatgatca ccgtatagat gagacatgga cattctcctg gtcaacccgt
     2461 ggcacaatgg aagagcggaa atacctcctt tgggacttca atgcctcgcc ggttcgctga
     2521 tgaatgatgg aattgaggcg gtcatcctcg acctcgacgt ctggccgaag gaagacgtcg
     2581 aaggcatcct ggaggagacg ctcggaaaca gccgaccccg aatcctgggg gtgggtgcgc
     2641 tttccgacag ctttacctcg gcgatggaaa cctgcgtctt ggccaagagt ctaaatgctg
     2701 acatcctgac tgtcctaggg ggaatgcatg caaccgtttt ggatgtacag gtcctcgaag
     2761 agcatcccca ggtcgacatc gtcgtgcgcg gcgaagggga aaagacgttt cttgagctcg
     2821 tccgtcgctg tttggattcc ggcgattgta aaggtgtcga cggaataagt tacagggatg
     2881 gaaaggatgt agttcggaat caagaccggg agttggagaa aaatctcgac cggttcccat
     2941 tgccggagcg cagtgcgcag gatgtctccc ggtaccgcac ccaaagtgta tcttcgagcc
     3001 gagggtgtcg ccgtaattgt tcgttctgct ccatccagtc gcagtatcgc cacaccgtga
     3061 gggtgagaag tccggagagc ctgatgcgcg agatcggtga gttggtttcc ctcggggcgc
     3121 gaaggattat gttcacagat gacaatttta cctttagcct ggaaagagta agaggcatat
     3181 gcaagtcgat aatagaaagg ggcttcgata gatgcgtcga attctatgcc gaaggttgtc
     3241 ttgaggacat ttgtaagaat cctctaatcg cacagattct cggtcagtcc ggttttcgtg
     3301 ccctctatgc gggagcggaa tcgggatcgc cgaaaattct tgcgcggtac ggcaaaaatc
     3361 tgtctccggt agatgtatta aagggggctt cctattgcct ggagcagaat gtcatgccag
     3421 tcttgaactt cattcttttg ggaccctggg atacggtcga tacgattcgg cagacgatag
     3481 acctcgctaa acagtccttc gaaaatggag cggaaattgc gtatgcagag atgctgattc
     3541 cttatccggg cacgcccgtc caggaagaat tggtcaggga cggtaagttc cggcagaggg
     3601 ataacgtatt ttactttgaa tcttacgagg ggatttcggt ggagtggttc ttgcggactt
     3661 gcgacctagc gagggatata acaagacaca caagaggtac ggaaagactt ttcgggatga
     3721 gaaaggccta tcatgaattg acgtgcctgc agtcgcttct cgaaggggac atacccgtcg
     3781 gattaaggga ttgttcaggc gtaagggaaa ctgaatatct tgagcggaaa atagaagact
     3841 ggtttttgct gacaaaagac ctgatggagg gcattatccc ggtttaactg cggcagtgga
     3901 tgagcggcat tgcctcatct gtgagaggag actgatatca agaaagaaag gggaggaagg
     3961 ggaatcatgg attttgatct gactgatgag cacaagatgt tgaaagacat ggcctacaag
     4021 tttgccgtga acgagatagc accgatatcg ctggaatgcg atagagagga gaggtacacg
     4081 ccggaaatcc gcaagaaggc ggccgagcag ggtctggtgg gtgcatgggt accggaacag
     4141 tacggcggcg ccggcgtggg attcctcggt aacacgatca tcacggaaga attatcccgt
     4201 gtggatatgg ggataggcct caacgtagta gcggcgagct tcggttgtga agcggtattc
     4261 tattttggca cggaagagca aaagcataag tatttgaccc ccgtctgcag gggcgagtgg
     4321 gtggccgccg gtgcctttac tgagcccaac gccggcacgg acgtcgccgg ttacaagact
     4381 cgggcggtgc gggacggcag cgactttgtg ataaacggca acaagatgtt catcaccaac
     4441 ggcagcgtct gtgacttcat ggtagcccaa tgtatcacta agcctgagga gaagaagcac
     4501 aacagcttca gccaaatcat cgtccccgct acggccaaag gtgtaacccg gaacaaactg
     4561 cacggcaagc tcggcatccg gtcgagcgat acagcggaga taaccttcga ggacgtccgg
     4621 gtcccgcagg agaaccttgt gggcaaggac ggccggggtt tccaccaact gatgcacttt
     4681 tttgacgtca caagggtcat ggtctcgggt caggcgatcg ggttatcgca ggcctgtctt
     4741 gaccaggcga taaaatacgc caaggagcgg acggccttcg gtgccccgat cggcagtttt
     4801 caggccacga tggccaagct aaccgagatg gcgatcaagg tagagtcctt gaggaccctc
     4861 gtctacaagg ccgcctggtt ggtggacgta ggccgtcccg actatcacct ggcggcgatg
     4921 gccaagtact taggcggtca gacggccgtc ttctgcgcga acgcggcggt ggagattcac
     4981 ggcggctacg gttacatgga agagtattcc gtccagaagt ggtaccgtga cgccaagata
     5041 ctggagctct acgaaggtac taaagaggcc gagatcatga ccataggacg ggtgctacag
     5101 agtaagtgaa agcacgaagc g
//
LOCUS       DAOTMX010000102         5064 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_13703_length_5064_cov_3.388900, whole genome shotgun sequence.
ACCESSION   DAOTMX010000102 DAOTMX010000000
VERSION     DAOTMX010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5064
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13703_length_5064_cov_3.388900"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="P5573_04030"
     CDS             complement(<1..342)
                     /locus_tag="P5573_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27263.1"
                     /translation="MEVKLSTKSLMAVFVILVFLAGAPVEVQAQQRQQQQQPSALQAI
                     PFRFQPYITVQEVYDSNLFLSSNNRTHDWITTVTPGLRVSQQDAFTGILLDVNGGYNW
                     YANNDDLDYWSA"
     gene            complement(355..969)
                     /locus_tag="P5573_04035"
     CDS             complement(355..969)
                     /locus_tag="P5573_04035"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HRT27264.1"
                     /translation="MKKRCVGSTVVVLLLALLFFVPFTHAQETKAAADKKDVKGEMPA
                     DTDSYVIGPEDSLYIHVWREELLTRTVPVRMDGKISLPVVNDVQAAGLTPLQLKEALT
                     EQLKEFVDNPEVSVIVMEANSYKVYVSGEVRTQGVLRLRTETTILEIIPMAGGFTEWA
                     DKSDITIIRKENGKEKRIRANYNRIVKGKDPAIKLKAGDVIVVN"
     gene            2313..3362
                     /locus_tag="P5573_04040"
     CDS             2313..3362
                     /locus_tag="P5573_04040"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HRT27265.1"
                     /translation="MLKRGATKKAQVMSLEEAVKTYIKDGCMIGFGGFTAMNRNPTAV
                     AWEMVRQGIKNVHCMDRHGSACSWILNAVGAIKIYETDWMGWGEMAGKLDVNLERNYK
                     AGKMILEDYSHGAMAMRFLAGAIGAPFIPYYAPQGSDLYNTKYDALGRAGLRGKDPKI
                     PKIKFQEMADPFYGAGNVMLLPAARLDVAVIHVAQCGEKGTARWRGIGTIDKEICFAA
                     DKVIVVCEELTPEADLRKNPENNQIPYFVVDAIVELPWGGYPSAVPFYYDYDAPFMRG
                     MDAASRNPEDLKKWLDDWVFGPKSWEEVMIKVGAKRMLELKADSTTGYSTRILRGKKP
                     APRMKMPLSVARSGY"
     gene            3382..4281
                     /locus_tag="P5573_04045"
     CDS             3382..4281
                     /locus_tag="P5573_04045"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HRT27266.1"
                     /translation="MAKTVEQLMELIDKIPDEAAKPGEFILPELMAIQIAHQVRDDDI
                     VFAGTGLPMVGIMTANMLNAPHALLIYEAGICDGKTMHVPMSVCDQRAANMSSSLGGL
                     VDTFGFFLQQGFVTLGFLGGAAIDKYGGVNVTSIGDYYKPSHRFTGSGGNSDIGTMAK
                     RTAFIILQEKRRFLERNEYTTTPGWWCWDFKTNEWKPKKEVWKGTPYADHGPVAVVTN
                     MGVYKFDNNGEIFLESFHPGVTVQQIQENCGFALNVKNVKGETKPPTYRELFVLREFV
                     DAELIFLPQKVEYPAAIKKLIES"
     gene            4583..>5064
                     /locus_tag="P5573_04050"
     CDS             4583..>5064
                     /locus_tag="P5573_04050"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRT27267.1"
                     /translation="MNLELTETQKMIQDTARNFAKSELEPVAAKLDQVGDRETFLKNL
                     RKLGELGLMAINVKSEYGGSEAGVVAFSLAVTEIARACASTAVTMSVNNMVCEVIQAV
                     GSEEQKRAYIPKIASGEYYAGGFGLTETSAGSDPAGMRTTAIDDGDSYVLNGNKIFIT
                     "
BASE COUNT         1293 a         1349 c         1320 g         1102 t
ORIGIN      
        1 ggcgctccag tagtcgagat catcgttgtt cgcgtaccag ttgtagccgc cgttgacatc
       61 cagaagtatc cccgtaaagg catcctgctg actgacgcga agaccggggg tgaccgtcgt
      121 tatccagtca tgggtccggt tattcgatga aaggaagagg ttgctgtcat agacctcctg
      181 cacggttatg taaggctgaa acctgaacgg gatcgcctgc aacgcagacg gctgctgctg
      241 ttgctgccgt tgttgcgcct ggacttccac cggggccccg gccagaaaca ccagaatgac
      301 gaaaaccgcc atgagactct tggtagataa tttcacctcc atgcccctcc tttttcagtt
      361 tacaacgatg acatcgcccg ccttcaattt gattgcggga tccttcccct tgacgatccg
      421 gttgtagttc gccctgatcc tcttttcctt cccgttttcc ttccggatga tggtgatgtc
      481 ggacttgtcc gcccactcgg tgaatccgcc cgccataggg ataatctcga ggatggtagt
      541 ctccgtcctg agacggagga cgccctgtgt cctgacttcg ccggagacgt agaccttgta
      601 gctgttcgcc tccataacaa tgacggaaac ctcggggtta tccacgaact ccttcaactg
      661 ctctgtcagg gcttctttca actgaagggg cgtcaaaccc gccgcctgga catcgttgac
      721 cacgggcagg gaaattttcc cgtccatcct cacggggacc gtcctggtca gcaactcctc
      781 ccgccacaca tggatgtaaa gagaatcctc cggcccgatg acgtagctgt ccgtgtcagc
      841 gggcatctcc cccttaacgt cctttttgtc tgccgcggcc ttcgtctcct gcgcgtgcgt
      901 gaaaggaaca aaaaacagga gcgcaagcaa taaaacaacc accgtgctac caacgcaacg
      961 ttttttcatg agtccaacct tccgatgttt tgtaacctgt cgcattattt attgattatt
     1021 aatctgaacg tacaccgctc atttaacttt ttcttgttga taaattttaa gtattcaccc
     1081 tcccgaaatc aatacgacga aagtatgttt ttacacccgg aattaattac gactttccta
     1141 cgaagaaagt attagatgtt tctaatacta ttcaaatata atcctttata atcaataact
     1201 tgcttggtac tcaaatagga gatcgggcaa cgacaagatg ccatcgagaa taaaaaaacg
     1261 gcattcgtct ccaacgctgc cccaccggct gctttgaaac cctattgtat tgaaatcgta
     1321 agattttctg ttttctttac tgtttattcc tcagtagcaa tggccttgcc acattgacaa
     1381 aaaaagatta gaatttcaat atatcgtatt tattcacttt tcgtcgtaat cgactgaatt
     1441 caaattctgc ttgatgacgt tttcacctcg atgccgttac aaaaacaggg cagcccggat
     1501 gcaacttctt ccaaaccgct gaatataaga atgaataaat aatgacctcc atgtcacggc
     1561 agtcgtgaca taaatgtcgc cggcagctta ttttccgatg gtcattctcc gaaaaaggaa
     1621 acgaaaaaac gactcagcgg gagatgtgaa tcggcttccc ctaaaaaaga caggtattaa
     1681 aatgatgtta aaatatacca gcggcagacg tcagagtaaa gcccaaattt tatttttcga
     1741 ttaatttccc cccgcataca cccgggcacg ccctggagac gccggcccga tcggcggtcc
     1801 atcacagcca ctaaaattcc aattttttct tgacagtcgc gacgggataa aatataaagc
     1861 ggctggtaca taaatgctct caaggagggc ggttttcacc acggcgaaac accccgccct
     1921 ctgcaaacaa acatacacgc catgaggcgg atacctctgc cgtctgaacg atcagaaagt
     1981 cgcaaaggct ttttgaaccg ctttcagttt tgatcgtcga cgactaacga tagcggacag
     2041 ggttcatcga gtaatcacaa cctcagaaga aaagaagcac aatccgatat acgtaagatt
     2101 tcgcccacag gccgccctgc acggcggttc agggaaagag cgctcgcgaa agcaccggaa
     2161 tcgcaattga gttgattcta cgcgcttttt attccaatca agaagggggg tgagtcctac
     2221 catctgaaac gtcgttttcc cgagtttcat gttttatcac atcgtgatca gtatcagagg
     2281 catttattca ccggtaaaaa aaggaggtaa caatgcttaa acgtggagca acaaaaaagg
     2341 cccaagtcat gtcactcgag gaggccgtca agacgtacat caaagatgga tgtatgatcg
     2401 gttttggcgg attcacggcg atgaacagga atccgacagc cgtcgcctgg gagatggttc
     2461 ggcagggcat caagaacgtc cactgtatgg accggcacgg gagcgcatgc tcctggatcc
     2521 tcaacgccgt aggggcaatc aaaatctacg agacggactg gatgggctgg ggcgagatgg
     2581 ccggcaaact ggacgtcaac ctggaaagaa actacaaggc tggaaaaatg atcctcgagg
     2641 attactccca tggcgccatg gccatgcggt tcctcgcagg cgcgatcggt gctccgttta
     2701 tcccctatta cgcgccccag ggatcggacc tctacaacac caaatacgac gccctcggta
     2761 gagccgggtt gaggggcaaa gaccccaaga ttccgaaaat caaattccag gaaatggcgg
     2821 acccattcta cggcgcgggc aacgtcatgc tgcttcccgc cgcgagactg gacgtggcgg
     2881 tcattcatgt ggcccagtgc ggtgagaaag ggacggcgag atggcgcggg atcgggacga
     2941 tcgacaagga gatctgcttc gccgccgaca aggtcatcgt cgtttgcgaa gagctcacgc
     3001 ccgaagccga cctgagaaag aaccctgaaa acaaccagat tccctacttc gtcgtggatg
     3061 ccatcgtgga gcttccctgg ggaggctacc cgagcgcggt gcccttctac tacgactacg
     3121 acgccccgtt catgagaggc atggacgccg catcccggaa tcccgaggac ttgaagaagt
     3181 ggctcgacga ttgggtcttc ggaccgaaga gctgggaaga ggtcatgatc aaggtcggcg
     3241 ccaagaggat gctcgaactg aaggcagaca gcacgaccgg ttacagcacc aggatcctca
     3301 gaggcaagaa gcctgccccc aggatgaaga tgcctctgtc cgtcgcaaga agcggttact
     3361 aaaaaaggga gggaataaac tatggctaaa acagtggaac aattgatgga gttgatcgac
     3421 aagattcccg atgaggctgc gaagccgggc gagttcatcc tgcctgagct gatggccatc
     3481 cagattgccc accaggtccg cgatgacgat atcgtcttcg ccggcacggg tcttcccatg
     3541 gtggggatca tgacggcgaa catgctgaac gcgccgcatg cgctgctcat ctatgaggcg
     3601 gggatctgcg acggaaagac gatgcacgtc cccatgtcgg tctgcgacca gagggcggcc
     3661 aatatgagct catcgctggg cggcctcgtg gacactttcg ggttcttcct ccagcagggc
     3721 ttcgttaccc tggggttcct cggcggcgcg gccattgaca agtacggcgg cgtcaacgtc
     3781 acatcgatcg gcgattacta caaaccctcc caccgcttca ccgggtccgg cggaaactcc
     3841 gacatcggaa cgatggcgaa gagaaccgcc ttcatcatcc tccaggagaa gagaagattc
     3901 ctcgagagga acgagtacac gacgaccccc ggctggtggt gctgggactt caagacgaac
     3961 gagtggaaac ccaagaagga agtatggaag ggtaccccgt acgccgacca cggacccgtg
     4021 gccgtcgtga ccaacatggg tgtctacaag ttcgacaaca acggtgagat cttcctcgaa
     4081 agcttccacc ccggtgtgac ggtgcagcag atccaggaga actgcggatt cgccctgaac
     4141 gtgaaaaacg tgaaaggtga aacgaagcct ccgacctaca gggaactctt cgtgctgagg
     4201 gagttcgtcg atgcagagct gatcttcctg ccccagaagg tggagtatcc cgcggccatc
     4261 aagaaactaa tcgagagcta acctgagaaa cgccggaaag gttggtgagg ggccccaaaa
     4321 gggcccttcc ccaacctttg ttttttgtcc attttcccgc ataaaaaccc ttgacccacc
     4381 tgtttgatgt gtgtatgaaa tcttgggcat taggcatagg gcattaggcg ttaggtcagg
     4441 catccaattg ttattgaacg tttctttttt tccatttgtc gcaacgcttg tcagaaacct
     4501 aaaaatgtca aaaagtgggc tttggaaaag gcttctgccc caaaggtctc acaaaatata
     4561 acccagggga ggtttggcat acatgaattt agaactcaca gaaacccaga aaatgattca
     4621 ggatacggcg agaaatttcg caaagtcgga gctagagccc gtcgccgcga agctcgatca
     4681 ggtgggggac cgggagacct ttctcaagaa cctgcggaaa ctcggcgaac tggggctcat
     4741 ggccattaac gtgaaaagcg agtatggtgg atcggaagcc ggcgtcgtcg cattcagcct
     4801 ggcggtcact gaaatcgccc gggcgtgcgc ctccacggct gtgaccatgt cggtgaacaa
     4861 catggtctgc gaggtcatcc aggccgtcgg ctcggaagag caaaaaaggg cctacatccc
     4921 gaagatagcc tccggcgagt attacgccgg cggcttcggt ctcacggaaa cgagcgccgg
     4981 ttcggatccc gcgggaatga gaacgacggc cattgacgac ggcgactcct acgtgctcaa
     5041 cggcaacaag atttttatca cgag
//
LOCUS       DAOTMX010000103         5062 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_13715_length_5062_cov_4.594767, whole genome shotgun sequence.
ACCESSION   DAOTMX010000103 DAOTMX010000000
VERSION     DAOTMX010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 5062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5062
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13715_length_5062_cov_4.594767"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..563
                     /gene="smpB"
                     /locus_tag="P5573_04055"
     CDS             93..563
                     /gene="smpB"
                     /locus_tag="P5573_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HRT27268.1"
                     /translation="MKHKQANEKLIARNKTANVNYFIEESLEAGISLLGTEVKALREG
                     KANLKDSYALVQDGEVFVHDLHIGQYSHANRMNHDPLRVRKLLLHAREIRRLYGKSRE
                     KGLALVPLRLYFKNGRVKLELGIGKGKKLYDKRESLKIKADKREMERGMRGKDW"
     gene            735..1088
                     /gene="ssrA"
                     /locus_tag="P5573_04060"
     tmRNA           735..1088
                     /gene="ssrA"
                     /locus_tag="P5573_04060"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1201..2070)
                     /locus_tag="P5573_04065"
     CDS             complement(1201..2070)
                     /locus_tag="P5573_04065"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRT27269.1"
                     /translation="MNYEMPLYRPPSEAYSLIFQVTIGCSHNRCAFCTMYKNKKFRVR
                     PWDDLKTDILEMALLNRATRRIFLADGDALVVDTDYMVKILNFLYDKFPYLERVSTYA
                     NPGNLLEKSAEELKTIRESGLQMLYFGIETGDDDLLVKVRKGATSQDLIASAGKAIDA
                     GFILSATVILGLAGKKRSEIHARETARVCSAINPHYLSALTLMLSDDNRYFFRCVGED
                     WEWMDKLEILRELRTMIEHFQLDSCIFRTNHASNYLPIKATLNQDKERLLKEIDNVLK
                     NPAWLRPEYLRAL"
     gene            complement(2144..2219)
                     /locus_tag="P5573_04070"
     tRNA            complement(2144..2219)
                     /locus_tag="P5573_04070"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2184..2186),aa:Phe,seq:gaa)
     gene            complement(2351..2824)
                     /locus_tag="P5573_04075"
     CDS             complement(2351..2824)
                     /locus_tag="P5573_04075"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HRT27270.1"
                     /translation="MTFFQYGGFGRRIMAFLIDNILLQTAATFVFLLGLSLVDSDMDL
                     ATLSSLPFLYYLIAVTMNLFYFTYFHSITGQTVGKRILGLRVITTAGEPLKARQAFLR
                     WVGYLVSAAFFYLGFIWVAFDSRKQGWHDKIAGTLVVRVDLHPEGVQPDLSAKIP"
     gene            complement(2821..3582)
                     /locus_tag="P5573_04080"
     CDS             complement(2821..3582)
                     /locus_tag="P5573_04080"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008692.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HRT27271.1"
                     /translation="MTPELTTPRLYLPEAMEVGSRLRLGADDRRYIGNVLRLRVGDRL
                     ILFDGSGKECDALIRELDAASAAVEIMGKRDVAPAGVSVTLAQALPKGSKMDIIVQKT
                     TELGVNRIIPFHSLRSIPRIPAEKSRLKTARWQKIAVEACRQCGRADIPEVTDVLSFE
                     EAIEAGGIGALKLLLWEEERERRVRDVLKDENVGQAEDIVVIVGPEGGFAPSEVDRAS
                     KLQYLSVGLGRRILKVETASIAILTIIQYERGQLG"
     gene            complement(3572..4501)
                     /gene="prmA"
                     /locus_tag="P5573_04085"
     CDS             complement(3572..4501)
                     /gene="prmA"
                     /locus_tag="P5573_04085"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HRT27272.1"
                     /translation="MPEKDRWIEIAISTPPQLVDALSNFLTESGAKGVFQEEVLEEAR
                     NVTDEPAETEELKAYFAANPANEKKIAAIHKYIDSLSEIFPDMKKPSVKTQLISDADW
                     GEQWKKYFKPIRLAENIVIKPTWERYTPLHREIIVDIDPGMAFGTGQHASTRMCLEAM
                     EDILLKDRKVQKWRVLDVGTGTGILAIAAAKLGAESVRGLDTDPKAVEVARKNVLING
                     VEDRVRIVNADVRKATGTFDLVVANLTAETLLKLKNHLVSLTGPGRYLIASGIIEQNA
                     KKVEESFARTLSIQRVLREKEWVCYVMKKGDDA"
     gene            4711..4929
                     /locus_tag="P5573_04090"
     CDS             4711..4929
                     /locus_tag="P5573_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HRT27273.1"
                     /translation="MKAEFTAIIEQAPEGGYWAICPEIPGANGQGETIEEAKDSLRQA
                     IELILEDRKADILRGLPEDAIREKVVIG"
BASE COUNT         1154 a         1306 c         1385 g         1217 t
ORIGIN      
        1 catcttcaag gccatgttcc atatcaaggt cgattatcta ccgggtctca aaaaataatg
       61 gtcgaagggc gggcagtgga ggtgcctccg gcatgaagca taaacaagcg aacgaaaagc
      121 tcatcgcccg gaacaagacg gcgaatgtca actactttat cgaagagtcg ctcgaggcgg
      181 gcatctctct tcttgggacc gaggtaaagg cgctcaggga agggaaggcg aacctaaagg
      241 acagctatgc cttggtgcag gacggggagg tcttcgtcca cgacctccat atcggccagt
      301 actcccacgc aaaccgcatg aaccacgacc cgctgcgcgt ccgtaagctt ctgctccacg
      361 caagagagat taggaggctt tacgggaaat ccagagagaa gggccttgcc ctcgtcccgc
      421 tgaggctcta tttcaagaac ggcagggtca agctcgaact cgggatcggc aaaggtaaga
      481 agctttacga caaacgcgag agcctcaaga taaaggccga caaacgcgag atggaaaggg
      541 gaatgcgcgg aaaagattgg tgatcgtgga gaatcttcgc tatcagaaga ccgcacgctt
      601 ctttgcttcc cgcgggcaat gatgtgagag ggtctctcgg gtctcatggc aaagaaaagt
      661 gagtacgggt tgactttgct gtctatgtgc tttataattt tcacattcat ccctttagaa
      721 tcgattcacc gaatgggggc gtaccggttt cgacggggat atggaagctg tagtggcata
      781 ccgaggttcc taccaggcct cgttaaacag gtaggaaaca tataagcgca accaaatacg
      841 attacgcttt agccgcatag acggctacgt tctcccggcc tcgcctatcg ggtcggatga
      901 gggcgtcaaa caggtaggat agccgaaccc gcctgcctga agcgggggaa gcgaaatcaa
      961 gacaggatag ctctcgataa atcctgcctt tgggagttgt cgagagtgaa tttagaacaa
     1021 aggctatgta tgtagaagct gcagcggaat gtttccggac gcgggttcga ctcccgccgc
     1081 ctccaccaat aaatatctgt agtatcaata agttggagaa aggttgttag cgggtaagaa
     1141 tggtgtggga agtgtctctt tgccacgtgc atcgaaaaga gtgcctgacc cgtcttctca
     1201 tcataaagca cgcaggtatt ccggcctcaa ccacgcaggg ttcttcaaga cattgtctat
     1261 ctctttcagg agcctttcct tatcctggtt tagggtggcc ttgataggga gatagttgga
     1321 ggcgtggttg gtgcgaaaga tacaggaatc tagctgaaag tgctcgatca tagtgcgaag
     1381 ttcgcggagt atttccagtt tatccatcca ctcccagtct tcccccacgc aacggaaaaa
     1441 gtaccgattg tcatcactga gcatgagggt taaagcggat agatagtgcg ggttgatagc
     1501 ggaacagacc cgtgcggttt ctcgtgcatg aatttcgctt ctcttcttcc cggcaagacc
     1561 gaggataacg gttgccgaaa gtataaagcc cgcatcgatg gccttccccg cgctcgcaat
     1621 caaatcttga gaagttgccc cttttcttac cttgacaagc aggtcgtcgt cgccggtctc
     1681 gatgccgaaa tacagcatct gaagaccgga ttcacgtatc gtttttagct cttcggccga
     1741 cttttccaag aggttgcccg ggttggcgta tgtgcttacc cgttcaaggt aagggaattt
     1801 gtcgtaaaga aagtttagga tcttgaccat gtaatcagtg tcaacaacca gtgcgtcgcc
     1861 gtcggcgagg aagatcctcc ttgtcgcgcg attcaaaagc gccatctcga ggatgtcggt
     1921 cttgaggtca tcccacggcc ggacacgaaa ttttttgttt ttgtacatgg tgcagaaggc
     1981 gcaccggttg tgagaacaac ctatggttac ctggaaaatg agtgaatacg cctcactcgg
     2041 cgggcggtat aacggcattt cgtagttcat ggtgatgtca tccttaactc agtgctacca
     2101 tgaaaaatgg gttagccact tagtggttaa cccatttaat taatggtggg tcagggcaga
     2161 attgaactgc cgacactcgg attttcagtc cgatgctcta ccgactgagc taccgaccca
     2221 tctctctcta ttctcaaaaa gcccgtcctt ccggcttacc cggtaatgac gaatctgccg
     2281 gattccacac cgattttcat cggagcgatt catcggtcgg agagacaaat attaaaaaac
     2341 ccctcttttg tcaaggtatt tttgcggaca ggtccggttg taccccctcg gggtgcaggt
     2401 cgaccctgac gacgagcgtc ccggcgatct tgtcgtgcca gccctgcttt ctgctgtcga
     2461 aggcgaccca gatgaagccg agatagaaaa aggcagcgga aacgagatac cccacccatc
     2521 tgaggaaggc ctggcgggcc ttcaacggct cccccgcggt cgtgatgacc ctcagaccga
     2581 gaatcctttt gcctacggtc tgcccggtta tgctgtgaaa gtaagtgaag taaaagaggt
     2641 tcatcgtcac ggcgataagg tagtacaaaa acggcaggga gctcaaggtt gcgaggtcca
     2701 tgtcggaatc gacgaggctg agcccaagca gaaaaacgaa cgtggccgcc gtctggagca
     2761 ggatgttgtc tatcaggaat gccattatcc tccggccgaa accaccgtat tgaaagaatg
     2821 tcatccgagt tggccccttt cgtattggat gattgtgagt atggcgatgg acgccgtctc
     2881 gaccttcagt atccgccggc caagaccaac ggaaaggtat tggagcttcg atgccctgtc
     2941 tacctcggag ggtgcgaatc caccttccgg ccccactatc acgacgatgt cctctgcctg
     3001 ccccacgttt tcatctttga ggacatcgcg taccctcctc tctctttctt cctcccagag
     3061 aaggagcttg agggcaccga taccgcccgc ctcgatggcc tcttcaaagg aaaggacgtc
     3121 cgtcacctcg gggatgtccg cccttccgca ttggcggcag gcttcgactg caatcttttg
     3181 ccaccgggcc gtctttagtc tcgatttctc cgccggaatt cttgggatcg accggaggga
     3241 atggaagggg ataatcctgt ttacgccgag ttccgtcgtt ttctggacta tgatgtccat
     3301 tttcgacccc ttgggaagtg cctgggcgag agttacactc accccggcgg gggctacgtc
     3361 cctctttccc atgatttcga cggctgccga agccgcatcc aattcccgga taagggcatc
     3421 gcattctttg ccgctgccgt cgaaaagaat cagtctgtcg cctaccctga gccttaatac
     3481 attaccgatg taacggcggt cgtcggcgcc gagtctgagc ctgctaccca cttccatcgc
     3541 ttccggtaga tacaacctcg gtgtggtcaa ttcaggcgtc atcccctttt ttcatgacgt
     3601 agcacaccca ctccttctcc ctaagaacac gctggatcga taaggtccta gcgaaggact
     3661 cctccacttt ctttgcgttc tgctcgatga tgcctgaagc gatcaggtat cttccggggc
     3721 ctgtcaacga gaccagatga tttttcagct tcaggagggt ttctgccgtc aggttggcga
     3781 cgacgaggtc gaaagtgccg gtagccttgc ggacatcggc gttgacgatc cttaccctgt
     3841 cttccacgcc attgataagg acgttcttac gggctacctc gacggctttt gggtcagtgt
     3901 ccaggccgcg gacgctctcc gctcccaatt tcgccgccgc gatggcaagt atgccggtcc
     3961 ccgttcccac gtcgaggacc cgccacttct gaaccttcct gtccttgagc aggatgtcct
     4021 ccatcgcctc caggcacatc ctggtcgatg cgtgctgtcc tgtcccgaag gccatcccgg
     4081 ggtctatgtc gacgatgatt tcccggtgaa ggggcgtgta tctttcccag gtcggcttga
     4141 tgacgatgtt ttccgccagg cggatgggct tgaaatactt tttccactgc tctccccagt
     4201 cggcatcgct gatgagctgg gtcttcacag acggtttttt catgtcgggg aagatctcgg
     4261 acaggctgtc gatgtattta tggatagccg cgattttctt ttcgttagcg ggattagccg
     4321 caaaataggc ctttaattct tccgtttccg ccggttcatc ggtgacgttc cgggcctctt
     4381 cgaggacctc ttcctggaag acgccctttg ctcccgattc ggtcagaaaa ttggagagcg
     4441 catcgacaag ttgaggcggt gtcgaaatgg cgatttcaat ccacctgtct ttttccggca
     4501 tctttttgcc cctttctgtg tcggcctgaa ggtgattccc cgagcataca ggggaaggac
     4561 cgattaatcg ttctataacc cgtttttctt attatttcaa ggaacttgaa attgtccggc
     4621 cttcgtggta gcgtaaaaag ccgcgttatc agggaagatt gtatcctatt cgggaggttt
     4681 gctgagagga gataaaagaa aggaggttca atgaaagctg aattcacagc aatcatagaa
     4741 caagcacccg agggtggata ctgggccatt tgtccggaga ttccgggtgc caacggacaa
     4801 ggagagacaa tcgaagaagc gaaggatagt ctgcggcagg ctattgaatt gatcctggaa
     4861 gaccgcaagg cggatatcct tcgcggactg cctgaagatg ccatccggga aaaggttgtg
     4921 attgggtgaa acgacgggat ttggagtgtg agcgggtgac tgagggttcc ggtagattga
     4981 gatactatcc cctctatctg aatatagtga acaaaaggtg caccgtcgtc ggcggaggag
     5041 aagtggcggc gaggaaggtg ga
//
LOCUS       DAOTMX010000104         4928 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_14147_length_4928_cov_3.686641, whole genome shotgun sequence.
ACCESSION   DAOTMX010000104 DAOTMX010000000
VERSION     DAOTMX010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4928
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14147_length_4928_cov_3.686641"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="P5573_04095"
     CDS             <1..639
                     /locus_tag="P5573_04095"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRT27274.1"
                     /translation="LVQTSVESMRRDGAPGGEGQDCDEIIDDLEFTIRELERVRDIIR
                     SLLGLSRQTTALVESVNLNRVVEDALMVLRNQYKGLDVVIARDYQEDLPEIEGNYANL
                     GQVVVNIIKNAIQALPKKEGKISVTTKILDNGKVCFECRDNGMGIPPENIRQIFDPFF
                     TTKEAGRGTGLGLYISNEIVRRHGGRIDVCSTVNEGCVFRVELPARQRRSPA"
     gene            887..1894
                     /locus_tag="P5573_04100"
     CDS             887..1894
                     /locus_tag="P5573_04100"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRT27275.1"
                     /translation="MVGLLVVDDEEGVRRSLKKVLEKDGYAVYLAEDGEKAIATVRDH
                     KDEIEVVVSDFRMPGLDGLETLAEIGKINPEITRIILTGYATMENAVEAVNSGIDGFV
                     LKPFGNLELREKIREYNLRKRLKQFVSEKVLAEMVKNGRSIVPRSQPVSIVFVDIRGF
                     SRLYEKLSPEEVSRLLSYSYFSPLDNIIHEYNGTLDKHIGDGIMGVFGAPVSYGDDAE
                     RAVLSAVRMRDEMVRTGQGVPFDGQGLSVGIGVSTGEAIAGIFGSNRKKEYTVYGFTV
                     NLAARLERLAGPNEVLICPDTYQRVRDIIRAEKIESVSIRGIDRKMDIFRVKGLTENT
                     H"
     gene            1951..2027
                     /locus_tag="P5573_04105"
     tRNA            1951..2027
                     /locus_tag="P5573_04105"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1985..1987,aa:Met,seq:cat)
     gene            2208..3548
                     /gene="trpD"
                     /locus_tag="P5573_04110"
     CDS             2208..3548
                     /gene="trpD"
                     /locus_tag="P5573_04110"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HRT27276.1"
                     /translation="MITAVVLFKVEKGKIPGLANQLGAIEDVVEVLSVTGEYDLMAKI
                     QVREYESLSDIVTDKMQNIDGVVSTRTMMAFKTYKFHELAVGAPSAMVVPPSAEEAEV
                     LESGKRTIKPILTKLTENFNLEKGEIDSLIDAINAGDLSDVQIAGFLVGLLSKGPSIK
                     EVAYIAQAMRRNCIPIKVSLKSNLTDTCGTGGGLTTFNVSTANAILTAAAGVPVAKHG
                     SRSISSPSGSADVLEALGVNIDQTPERAARMIEEIGISFIYAPNFHPVMLKVFGPENQ
                     LGIKTIFFTIIGPLINPAGARNHTIGVYKPELVHMMADVVAEMDFNHVIVAHGLDGLD
                     EISLIGRTSIAEVKGKKVKYYEVSPEDFGLKRCILAEIAGGPPAFNAEVIRNIFSGAE
                     KGPRRDFLTLNNAAALYVSGKAKSIQDGLALSRSLLDSGAAQRKLQELVERSNA"
     gene            3583..4287
                     /locus_tag="P5573_04115"
     CDS             3583..4287
                     /locus_tag="P5573_04115"
                     /inference="COORDINATES: protein motif:HMM:NF012444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol-phosphate synthase"
                     /protein_id="HRT27277.1"
                     /translation="MKLTLSESIRLRQQEGRFPVISEIKVRSEKEGDLLRGRDPAQLA
                     RQMAGQPVAGISVVTEPEHFGGSMDILRAVAGAVSVPILHKDFITTERQVEESAAYGA
                     SAVLLITAMLEADRMIRLIEKARGLGLESLVEAHNLEEIERVSRLQFDLMGINNRDIT
                     IFEVDDDDVGRTETLARACRSGRPLISESSIGSAADVRRAGKSGADAVLVGTALLKPG
                     SPVELLSEMIAVGWPV"
     gene            4284..>4928
                     /locus_tag="P5573_04120"
     CDS             4284..>4928
                     /locus_tag="P5573_04120"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HRT27278.1"
                     /translation="MTRIKICGVTGEEEIRVCVAAGAHALGFVVEYPIDVPWNLKRQR
                     AKDLMAAVPPFVSRVIVVGDDPQRIIECTELLKPHAVQLHGDEPIAVTAELVAAVNRL
                     GAQVLKPLRFSVETGKCRSECEDPVEAARLIERTGADAVVLDSVSEARPAGTGSSVDW
                     SLARKIRDSLRIPVILAGGLNAGNVAQAVRAVLPYGVDVISGVEDASGRKVPEKV"
BASE COUNT         1124 a         1310 c         1540 g          954 t
ORIGIN      
        1 ctcgtccaga ccagcgttga atccatgaga agggatggcg caccgggagg agaaggacaa
       61 gactgcgatg agatcatcga tgacctggaa ttcaccatca gagaactcga aagggtgcgt
      121 gacataatca gaagcctcct cggcctttcc cgtcagacga cggctttggt ggaatcggta
      181 aatctgaaca gagttgtcga ggatgccctg atggtccttc gtaatcagta caagggcctt
      241 gacgtcgtga tagccaggga ttaccaggaa gacctcccgg agatagaggg caattacgcc
      301 aacctggggc aggtcgtggt caacataata aagaatgcga tccaggcctt gccgaaaaag
      361 gaagggaaaa tcagcgttac gaccaagatc cttgataacg gaaaagtgtg ttttgagtgt
      421 cgtgacaacg ggatggggat acccccggaa aacattcggc agatcttcga cccctttttt
      481 acgacaaaag aggcgggcag aggaactggt ctcggccttt acatctccaa tgagatcgtg
      541 aggaggcacg ggggtcgcat agatgtctgc agcacggtga acgaaggatg cgtcttccga
      601 gtggagctgc ccgctcgaca gcgccgctcg cctgcatgac gtatcaagac ctttgaattt
      661 gtggttaaat cgccttacgg tcattccgtg cttgacttaa gcctgccccg tactcgatac
      721 gggggaatct ggtgttttta atctgttctg gattttgccc cgattttcac cggagtgacg
      781 agtcggaatg acatgaatgg gtgtttttca aaggtctcgt gtcctgattc atggggcgtg
      841 gtcgtttaag gacagtggga taggttggaa gtttttggag ggtgctatgg taggtttatt
      901 ggttgttgac gacgaggaag gggtcaggag gtcgctgaag aaggtcttgg agaaagacgg
      961 ctacgccgtt taccttgccg aagacggaga aaaggctatc gcaacagtcc gcgaccacaa
     1021 ggatgagata gaggttgtcg tgtccgactt caggatgccc ggcctcgacg gcctggagac
     1081 cctcgcggaa atagggaaaa tcaacccgga aatcacgaga atcatcctga cgggatatgc
     1141 caccatggaa aatgccgtcg aggccgtaaa ttcgggtata gacggcttcg tgctgaagcc
     1201 cttcgggaat ctcgagctga gagaaaagat ccgcgagtac aatctccgca aacgtttgaa
     1261 acagttcgtt tccgaaaagg tccttgcaga aatggtcaaa aacggtagga gcatcgttcc
     1321 gcggagtcaa ccggtcagca tcgtgttcgt cgacatcagg ggcttttcac gcctttatga
     1381 gaagctgagc ccggaggaag tgtcgcggct gttgagctac agctactttt cgccccttga
     1441 caacatcatt cacgagtaca acgggacgct ggacaaacac atcggcgacg ggataatggg
     1501 tgtcttcggc gcgccggtca gttacggcga cgacgcagaa agggccgtgc tgagcgccgt
     1561 ccgcatgagg gacgagatgg ttagaaccgg ccagggggtc cctttcgatg gacaggggct
     1621 ttccgtgggg atcggggtca gcaccggcga ggcgattgcc gggattttcg ggtctaatcg
     1681 gaagaaggag tacacggttt acggttttac cgtcaatctg gccgcccgac tggagcggct
     1741 cgccgggccg aacgaggtcc tgatatgccc ggatacgtat cagcgagtcc gggatattat
     1801 cagggcggag aagatcgagt cggtcagtat cagggggatc gacaggaaga tggacatttt
     1861 cagggtcaag ggattgacag aaaataccca ttgacatatc agggcttatc aattaattat
     1921 gtacgtcacg ttgaatgtga ttactgatga cgcggggtgg agcagtccgg tagctcgttg
     1981 ggctcataac ccaaaggtcg gaggttcaaa tcctcccccc gctaccaata aaaataaagg
     2041 ggttaggcga attgcctaac ccctttattt tttatgcgct cccccgtatg ctacatctta
     2101 cacagtccga ttgctatgac ctgtctcagc ggaggtaagc tcgatttcag gccggccaca
     2161 ggagacggcg aggaacataa aaaccaatat ggaaaggagc aaggcccatg attacagcgg
     2221 tcgttttgtt caaggtggaa aaggggaaga tccccggctt ggccaaccag ttaggggcga
     2281 tcgaagacgt cgtggaggtc ctgtcggtga ccggcgagta tgacctcatg gcgaagatcc
     2341 aggtccggga atacgagagc ctctcggata tcgttaccga caagatgcag aacatcgacg
     2401 gagtcgtatc cacccggacc atgatggcct tcaagactta taaatttcat gagctcgccg
     2461 tcggtgcccc ctcggccatg gttgtcccgc cgtcggcgga ggaggccgag gtcctggaaa
     2521 gtggcaaaag gacgatcaag ccgatcctca ccaaactgac ggaaaacttc aacctggaga
     2581 agggcgaaat cgacagcctc atcgacgcca tcaatgccgg cgacctctcc gatgtgcaga
     2641 tagcgggctt ccttgtcggc ctcctcagca aaggtcccag catcaaggaa gtagcctaca
     2701 tagcccaggc gatgcgaagg aactgcattc ctatcaaggt ctctctgaaa tcgaacctga
     2761 cagacacctg cggaaccggc ggaggtctga cgaccttcaa cgtcagtacc gccaacgcaa
     2821 tcctgacggc cgcggctggt gtcccggtgg ccaagcacgg cagtcgttcg atctcgtctc
     2881 cctcgggaag tgcggatgtc ctggaggccc tgggcgtcaa tatcgaccag acaccggagc
     2941 gagccgcccg gatgatcgaa gaaatcggca tcagctttat ctacgcgccg aatttccatc
     3001 ccgtcatgct caaggtcttt ggaccagaaa accagctcgg catcaagacg atcttcttca
     3061 cgatcatcgg cccgctcatc aaccccgccg gggccaggaa ccacacgatc ggcgtctaca
     3121 agcctgagtt ggtccatatg atggccgatg tcgtcgcgga gatggatttc aaccacgtaa
     3181 tcgtggccca cggcctggac ggcctcgacg aaatctccct gatcggcagg acatccatcg
     3241 cggaggtcaa ggggaagaag gtcaaatact acgaagtctc tcccgaggat ttcgggttga
     3301 agcgctgcat cctcgccgag atcgccggcg gtcctccggc attcaatgcg gaggtcatcc
     3361 gtaatatctt ctccggtgcc gaaaaagggc cccggcggga tttcctgaca ctcaacaatg
     3421 cagccgccct ttatgtgagc ggcaaggcca agagcattca ggacggactc gccctgtccc
     3481 gctcgcttct cgattcgggc gcagcgcagc gcaagctcca ggaactggtg gagaggtcga
     3541 atgcataaaa gttcagggtt ctgagtgatg aaagcacatg gagtgaagtt gacgctctca
     3601 gagtcgatca ggctacggca gcaagagggg cgcttcccgg tgatctccga gatcaaggtg
     3661 cgctcggaga aggagggcga cctgttgcgc ggccgcgacc cggcgcagct cgcgcgacaa
     3721 atggcggggc agccggtcgc ggggatcagc gtggttaccg aacccgagca tttcggcggc
     3781 agcatggaca tcctgagggc ggttgccggt gccgtttccg tcccgatcct ccacaaggac
     3841 ttcatcacga cggaacggca ggttgaggaa tcggccgcct acggcgcctc ggcggtcttg
     3901 ctgatcacgg ccatgctcga ggcggaccgg atgatccgcc tcatcgagaa ggccagaggg
     3961 ctcggactgg aaagcctcgt cgaggcgcac aaccttgagg agattgaaag ggtgagccgg
     4021 ctccagttcg acctgatggg gatcaacaac cgggacatca cgatattcga ggtcgacgat
     4081 gacgatgtgg gccggacgga aacactggct cgtgcctgcc ggagcgggcg gcccctcatc
     4141 agcgaaagct cgatcggctc cgccgcggat gtccggcgcg cgggcaagag cggcgcggac
     4201 gccgtcctcg tcgggaccgc tctcttaaaa ccgggcagtc cggtcgaact cttgagcgag
     4261 atgatcgcgg tggggtggcc cgtatgacgc gcatcaagat atgcggtgtg accggcgaag
     4321 aagaaatccg cgtctgcgtg gcggccgggg cccatgccct gggcttcgtg gtggagtacc
     4381 ccatcgatgt cccctggaac ctcaaaaggc aacgggcgaa agacctgatg gccgcggtcc
     4441 cgcccttcgt ctcgcgggtc atcgtggtcg gggacgatcc gcaaaggatc atcgagtgca
     4501 cggaactcct gaaaccacat gccgttcagt tgcacgggga cgaacccata gctgtgacgg
     4561 cggagctcgt agccgccgtc aataggctgg gtgcccaggt actcaaaccc ctgcgctttt
     4621 ccgtcgagac cggaaagtgc cggtccgaat gcgaagaccc ggtcgaggcc gctcggctga
     4681 tagaaaggac gggggcggat gccgtggtgc tcgattcggt cagcgaggcc cggcctgcgg
     4741 gaacgggcag cagcgtggac tggagtcttg cccggaagat ccgcgatagc ctgcggatac
     4801 cggtcatcct ggcggggggg ctcaatgccg gcaatgttgc gcaggccgtc agggcggtcc
     4861 tcccctacgg cgtcgacgtc atcagcggcg tggaggatgc ctccgggagg aaggtcccgg
     4921 aaaaggtc
//
LOCUS       DAOTMX010000105         4922 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_14168_length_4922_cov_3.848572, whole genome shotgun sequence.
ACCESSION   DAOTMX010000105 DAOTMX010000000
VERSION     DAOTMX010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4922
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14168_length_4922_cov_3.848572"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..1198
                     /gene="rodA"
                     /locus_tag="P5573_04125"
     CDS             89..1198
                     /gene="rodA"
                     /locus_tag="P5573_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HRT27279.1"
                     /translation="MKFDRRLLLNFDLVFLLIVMTICAIGLLNMYSAGFNVLSGKSGP
                     LCLRQSVWFLTGLVFMVVMFSIDYRSFITYAYVFYGISLALLVIVFIVGDVTRGTQRW
                     LALGWFSFQPSELMKLAAIVALARYFAMNEKEGAYGLRELVVPFLMVFVPFVLIMKQP
                     DLGTALILLIIFASMALFVGVKGKTVMIVVTTGILLLPFSWYFLKDYQRERLLAFFDP
                     EGDPLGSGYHILQSIIAVGSGGFFGKGFLKGTQSQLRFLPEHQTDFIFSVFAEEWGFL
                     GSVVVMILYLTLIVWGLKIARQSRDYLGTLIAFGVTMLLFWEVVINVGMVVGFFPVVG
                     IPLPFMSYGGSSMVVMMMGVGLLMSVSTRRFILQP"
     gene            1275..1715
                     /locus_tag="P5573_04130"
     CDS             1275..1715
                     /locus_tag="P5573_04130"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HRT27280.1"
                     /translation="MVGKKKIEEVKAFLGKGTEFEGKLLFTGSVRIEGKFKGQVVGGG
                     TLNVGEGARIEGEVFVDNVLIGGEVVGNLEIKKRTEIFSRGNLSGELKTMVLVIHEGA
                     VFEGHCQMKAEPALRAVERGRHPEEEVSWQKKESWSSDPGAITG"
     gene            1664..2017
                     /locus_tag="P5573_04135"
     CDS             1664..2017
                     /locus_tag="P5573_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannose isomerase type II C-terminal
                     cupin domain"
                     /protein_id="HRT27281.1"
                     /translation="MAEKGELEFRPWGHYRVIGEAENHKVKRVTVYPGARLSLQMHRL
                     REEHWFIVQGEGLVSREDVNLKVLKGESVDIPRGAFHRILNTGKEELVFIEVQTGDYF
                     GEDDIERTADDYGRA"
     gene            2256..2726
                     /locus_tag="P5573_04140"
     CDS             2256..2726
                     /locus_tag="P5573_04140"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HRT27282.1"
                     /translation="MKVVKKGKKSMVSIDYTLFIQIVNFLALIAILHVLLYKPILRIM
                     QERENRLKASEEEVKSLYQTIERKTAEYEEKIRLAKMEAMNQRNEIQKQGALEAQRII
                     EAAREEISRMTEEFKEKLAKEMEEARKILAAQSRNISFEIAERVLGRTFDEQRI"
     gene            2710..3315
                     /locus_tag="P5573_04145"
     CDS             2710..3315
                     /locus_tag="P5573_04145"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HRT27283.1"
                     /translation="MNKESKIGGRFKGRFIPILSVVIILIWSAVVFAAGGGEEGGHST
                     WADFAWRMFNFIVLVAILYMLTWKHIKNFFVGRRAAIKAALEEAVRAKEEAEAKFKEY
                     SERLEKASSEIEGISEMIKAQGVVEKEKIIESAKKTAEKMKEDSRARMDQELKKAINQ
                     LRLEASVLAVQTAEEILSKTIKKEDHERMVKETIDRMVKLN"
     gene            3312..3857
                     /gene="atpH"
                     /locus_tag="P5573_04150"
     CDS             3312..3857
                     /gene="atpH"
                     /locus_tag="P5573_04150"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004402.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HRT27284.1"
                     /translation="MSSGDLANRYSQALFEIAKETNSYEKYFEELKRFSAVLEENESF
                     REFLFNPIFDRDDKKAVVGEVLEKLGIAGVVANFLKLLVDKGRINILEDILNAYQYLM
                     DQALNKARVSVKTAYPLSDDLVAEVKKTLEGLTKKRVEMAIQEDPSLLGGIVVKIGDT
                     VYDGSVKAQLNKIRELLGEEI"
     gene            3861..>4922
                     /gene="atpA"
                     /locus_tag="P5573_04155"
     CDS             3861..>4922
                     /gene="atpA"
                     /locus_tag="P5573_04155"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004723422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HRT27285.1"
                     /translation="METIRAEEISKIISKQIKEYEKKLDVSETGTVLSVGDGIARVYG
                     VENAMAMELLEFPGGILGMVLNLEKDNVGVAILGEDIHIKEGDIVKRTGRITQVPVGD
                     ALLGRVIDATGRPIDGKGPIEATEFRRIEMVAPGVIARQPVNEPMYTGIKAIDAMTPI
                     GRGQRELIIGDRQIGKTAIGVDAIIRQKDTGVKCIYVAIGQKKSTVAQVVESLRKNDA
                     MSYTCVVAACASDPATLQYIAAYSGCSIGEYYRDREQDALIIYDDLSKQAVAYRQISL
                     LLRRPPGREAFPGDIFYNHSRLLERAARVNKDYGGGSLTALPIIETQAGDVSAYIPTN
                     VISITDGQVYLEPSLFYSGI"
BASE COUNT         1280 a         1049 c         1459 g         1134 t
ORIGIN      
        1 ttgcggccgc acccttggcg aggaagataa tcgacgccta tttcaagggt aaagacgagc
       61 agaagaaggc ggcacaggag ggagaagcgt gaagtttgac cggcggttac tgcttaattt
      121 tgacctggtt tttcttctca tcgtcatgac gatctgcgcc atcggccttt taaacatgta
      181 cagcgcaggc ttcaacgttc tcagcggcaa gtcggggcca ctttgtttga ggcagtcggt
      241 atggttttta acgggccttg tattcatggt ggttatgttc agcatagact accgttcttt
      301 tatcacgtac gcctatgttt tctacggaat ttctctggca cttcttgtca tcgtcttcat
      361 cgttggtgat gtgacacggg gaacgcagcg gtggttggcc ctgggttggt tttcctttca
      421 gccctcggaa ctgatgaaac ttgccgccat cgtcgccctt gccaggtact ttgccatgaa
      481 cgagaaagag ggggcatacg gcctgaggga gcttgtcgtg ccttttctta tggttttcgt
      541 gccctttgtc cttatcatga agcagcccga cctcggaacg gcgctgatcc tgttgatcat
      601 ttttgcctct atggcgctct tcgtcggggt caagggcaaa accgtcatga tcgtggtcac
      661 gactgggatt ctattgttgc ctttcagttg gtatttcctg aaagactatc agcgcgagcg
      721 tctacttgcc ttttttgacc cggaaggaga ccccctcggg tcgggatatc atatccttca
      781 gtccatcatt gccgtaggct caggaggttt ttttggaaaa ggatttctga aagggacgca
      841 aagccagttg cgtttcctcc ccgagcacca aacggatttt attttttcag tttttgccga
      901 ggagtggggt ttccttgggt ccgttgtggt catgatcctc tacctcacgc taatcgtctg
      961 gggtctgaaa atagcgcgcc aatcacgaga ttacttggga acgttgatag ccttcggcgt
     1021 tacgatgctc ttattctggg aagttgtgat caatgtggga atggtggtag ggttctttcc
     1081 tgtggtcggt attccgttgc ctttcatgag ttatggaggt tcttcgatgg tagtcatgat
     1141 gatgggtgtg gggttgctca tgagcgtcag tacgagacgg tttattctcc agccgtgatt
     1201 cgtgattttg aagccgagcg atcgcaaaaa ccggcctgct ttaagcagac gcaggtaaag
     1261 ggagggagtt gacgatggtg gggaagaaaa aaatcgaaga ggtgaaggcc tttcttggaa
     1321 aaggcactga attcgagggg aagctccttt ttaccggttc agtcaggatc gagggaaaat
     1381 tcaaaggcca ggtggtcgga ggtggcaccc tgaacgttgg tgaaggtgct cgcatcgaag
     1441 gggaagtctt cgtcgacaat gttctcatcg gtggggaagt agtcggaaac ctggagataa
     1501 aaaagcgaac ggagatattt tcacggggga atctgagcgg ggagcttaag acgatggttc
     1561 ttgtcattca cgagggcgca gtctttgaag gacattgtca gatgaaagcc gagcccgcat
     1621 tacgggccgt ggaaagaggc aggcaccctg aggaagaggt atcatggcag aaaaaggaga
     1681 gctggagttc cgaccctggg gccattaccg ggtgatagga gaggcggaaa accacaaggt
     1741 gaagagggtg accgtttacc cgggagcgcg actgagtctg cagatgcacc ggcttcgcga
     1801 agaacactgg tttattgtac agggtgaagg tctagtttcc cgggaggacg tgaacctgaa
     1861 ggtattaaaa ggagagtcgg tggacatccc taggggagcg tttcaccgga ttctgaacac
     1921 cgggaaggaa gagttggtct tcatagaggt tcaaacaggc gattatttcg gcgaggacga
     1981 tatcgaaagg acggcggatg attacggacg ggcctgaaaa aacactttat tttccgagac
     2041 agaatcctgc tgcaaaaggc aaaaaagact tgacattgaa tgaacgttat gattagtagt
     2101 gcccgcttta gcgatggggt gtgaaaggtt gacggggata atgaataacc tgtgtatata
     2161 tttcgtgaaa ggtgtcctgc gcctttctgc atatgtaggc acagcgtgga tggtctaaac
     2221 cgggtgaggt atttttcgct cggttttttt gttttttgaa ggttgttaaa aaggggaaaa
     2281 agagcatggt cagcatagat tatacgcttt tcatccagat tgtgaatttt cttgcgctta
     2341 tcgcgatatt acatgttctc ctgtataaac cgatcctcag aatcatgcag gaaagagaga
     2401 atcgcttaaa ggcatcggag gaggaggtca agtcgcttta ccagacaatc gaacgaaaaa
     2461 cggccgaata cgaggaaaaa atcaggcttg ccaagatgga agctatgaac cagagaaatg
     2521 aaatacagaa gcaaggagct ctggaagccc aacggatcat cgaagcagcc cgcgaggaaa
     2581 tctctcggat gacagaagaa tttaaagaaa agttggcgaa ggaaatggag gaagcccgaa
     2641 agatcttggc tgcccagtca aggaacatct cctttgagat cgccgaaagg gttctgggga
     2701 ggacgttcga tgaacaaaga atctaagata ggcggacggt ttaagggccg ctttatccct
     2761 attctgtctg tagtgattat cctgatatgg tcggcggtgg tctttgccgc cggaggagga
     2821 gaagagggcg ggcacagcac atgggccgat tttgcttgga ggatgttcaa tttcatcgtt
     2881 cttgttgcca ttctgtatat gctgacctgg aagcatatca agaacttctt cgtcgggagg
     2941 cgggcagcaa tcaaggccgc actcgaagaa gcggttagag ccaaagaaga ggcggaggcg
     3001 aagttcaagg agtactctga gaggctggaa aaggcgtcga gtgagatcga ggggatctcg
     3061 gagatgatca aggcccaggg agtcgtggaa aaggaaaaga tcatcgaaag cgccaagaag
     3121 acagcggaaa aaatgaagga ggacagccgg gcgagaatgg accaggaatt gaagaaagcc
     3181 atcaaccagt tgaggcttga ggcgtcggtc cttgccgttc agacggctga agagattctt
     3241 tccaagacca taaagaaaga agaccatgag aggatggtta aagaaaccat agataggatg
     3301 gtgaagctga attgagtagt ggcgatcttg caaaccggta cagtcaggcg ctcttcgaga
     3361 tagcgaagga aacgaacagc tacgaaaaat attttgaaga actaaagagg ttttctgccg
     3421 ttctggaaga aaatgagagt ttcagagaat ttctcttcaa cccgatcttc gacagggacg
     3481 ataaaaaggc agttgtcgga gaagtgctgg agaaattggg tatcgcggga gtcgttgcca
     3541 attttttgaa gctcttggtg gacaagggaa ggatcaatat attggaagac atccttaacg
     3601 cttaccagta cctgatggac caagccctga acaaggctcg ggtgagcgtg aagaccgcct
     3661 atcccctttc cgatgacctc gtcgccgaag tcaagaagac actcgagggt ctgacaaaga
     3721 agcgggtcga aatggcgatc caagaagacc cctcacttct cggcggcatc gtagtcaaga
     3781 taggtgacac agtttacgac ggcagtgtta aagcccaact gaataaaata agggaactct
     3841 taggggagga gatatagagg atggagacga tcagggcaga ggaaatcagt aagataattt
     3901 ccaagcagat taaggaatac gaaaagaaac tcgacgttag cgagaccggt acagtcctgt
     3961 ccgtcggtga cggtattgcc agggtatacg gtgtggaaaa cgcgatggca atggaacttt
     4021 tggagtttcc cggtggtatt cttgggatgg tgctgaacct ggaaaaggac aacgtcggtg
     4081 tagccattct cggcgaagac attcacatca aagaagggga tatcgtcaag cgaacgggcc
     4141 gcattactca ggtgccggtg ggcgacgccc tcctcggccg tgttatcgat gccacgggaa
     4201 gaccgatcga cggtaaggga ccgatagagg caacggagtt tcgccgtatc gagatggtgg
     4261 ccccgggcgt gatcgcaagg caacccgtca acgagcctat gtacaccggt ataaaggcca
     4321 tagatgccat gacacccatc ggacgcggcc aacgtgaact gatcatcggt gaccgccaga
     4381 tcgggaagac ggcgatcggt gtcgatgcga tcatcagaca gaaggacacg ggcgtcaagt
     4441 gcatctacgt agccatcggg cagaaaaaat cgaccgtggc gcaggtcgtg gagtcgttga
     4501 gaaagaacga tgccatgagt tatacctgcg tagtcgcagc gtgcgcaagc gacccggcga
     4561 ccctgcagta cattgccgcc tattcgggtt gcagcatcgg tgaatattat cgagaccgcg
     4621 agcaggacgc actcatcatc tatgacgacc tttccaaaca ggccgtggct taccggcaga
     4681 tatcgcttct cctccgaaga ccgccgggac gtgaggcctt tcccggagac atcttttaca
     4741 accattcaag gctcctggaa cgcgcggcgc gggtgaacaa agattacggc ggagggtcgt
     4801 tgacggccct accgatcatc gagactcagg ccggcgacgt ttcggcctac atcccaacga
     4861 acgtcatttc catcacggac ggtcaggtct atctcgaacc gagccttttt tactcaggta
     4921 ta
//
LOCUS       DAOTMX010000106         4903 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_14236_length_4903_cov_4.995050, whole genome shotgun sequence.
ACCESSION   DAOTMX010000106 DAOTMX010000000
VERSION     DAOTMX010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4903
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14236_length_4903_cov_4.995050"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..1364
                     /locus_tag="P5573_04160"
     CDS             135..1364
                     /locus_tag="P5573_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRT27286.1"
                     /translation="MISIPSTFKISFRALRVNKMRSGLTMLGIIIGVGAVITMVAIGT
                     GASLRISQQIASVGSNLIVILPGSTTSGGIRMGLGSQQTLTKGDADAILKECPAVAEV
                     APIMNGVAQVVYGNMNWSTGVIGTTPSILEVRDWPLFSGRNFTEQDVRSAAKVCLLGQ
                     TVVDNLFGAADPVGQIVRIKKLPFTVVGVLDRKGQSQHGQDQDDTIFIPVTTAQKKIF
                     GTAFPGMVRTIMVKARSTEDLDAAERQITALLRQRHRIGQKADNDFSIRNLTQMMQVA
                     EQSTRVMTMLLGAIASVSLLVGGIGIMNIMLVSVTERTREIGIRMAVGAKTWDIRLQF
                     IIEALSLSLIGGFVGIAAGVITAEMISFFTGWSTVVSPLAVLTAFGFSGLVGIFFGFY
                     PAYKASLLNPIEALHYE"
     gene            1564..2007
                     /locus_tag="P5573_04165"
     CDS             1564..2007
                     /locus_tag="P5573_04165"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRT27287.1"
                     /translation="MVWPSVVRYGRMVDPFDEIQRLQQEMNRLFSGFGPAVVYEFPPI
                     NAWVSEDDAVLTAELPGIDPEKLDISVAGDTLTLSGERAPASLGEGETYHRQERNFGK
                     FARTLRLPFQVDPTKVEAKYEKGILRITLPRSEQDKPKKIAIKTN"
     gene            2027..2416
                     /locus_tag="P5573_04170"
     CDS             2027..2416
                     /locus_tag="P5573_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRT27288.1"
                     /translation="MAREKTQARMPVEAERTKEKRVFVPRVDIFETKDGIVIAADMPG
                     VDDKSIDVTLEKNILTIKGRVEAKPVEGHSLAYAEYDEGDYERLFTLTDEVDREKIEA
                     KVKNGVLEVILPKAEPAKAKKIAIKAE"
     gene            2554..2964
                     /locus_tag="P5573_04175"
     CDS             2554..2964
                     /locus_tag="P5573_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRT27289.1"
                     /translation="MNRIFDDFYRSFDLAPFAGAEERLGFFTPAVDVVEGKKDITVKV
                     ELPGLDEKDVEVLLSDDTLTIRGEKKQEKEESTGDYYRMERTYGRFERVIPLPENVDP
                     AGIEAKFKKGVLSIQMPKRAEIEDKAKKIEIKAE"
     gene            3247..3831
                     /locus_tag="P5573_04180"
     CDS             3247..3831
                     /locus_tag="P5573_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27290.1"
                     /translation="MRTTLRSLCILRILCRADGPVEESFIQSGLFLLQEAMGETLDCN
                     FYLDMQGPRSREVLETLITLEREGKVAIGGSPRGLLVEATEKGKQFIHQGGDWGVFFD
                     VPPVRVPEAQIDAVLSLREKEAPLEMAALGTALFLALSAASPGNILEELDKAKSEGRL
                     AADLDDRSVVEGFRRLRRYGLGPARKGERTKPRR"
     gene            3847..4221
                     /locus_tag="P5573_04185"
     CDS             3847..4221
                     /locus_tag="P5573_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27291.1"
                     /translation="MKRYGWLAVGLCLLMVFSGCGYKSQKQGAMINEASVDKVIVDGQ
                     TTKQEVVLEFGPPTKTMEGERMFFYTWTETSKSSIPLYGGETSVTKDLIILFDDNGVV
                     KSHKITKTARESKAGFGGQKEK"
     gene            complement(4246..4467)
                     /locus_tag="P5573_04190"
     CDS             complement(4246..4467)
                     /locus_tag="P5573_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27292.1"
                     /translation="MYIFRSKAGTFTIGSDPTVSGCYELCIGGMWLGTYETADKAAKD
                     VFLRCTGWLDWDSRKDTDCPRELGEWEVL"
     gene            complement(4501..>4903)
                     /locus_tag="P5573_04195"
     CDS             complement(4501..>4903)
                     /locus_tag="P5573_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HRT27293.1"
                     /translation="HRKGATRSFPPGHPAVPAVYRRHGQPVLIPGDMGVGSYVLAGTE
                     KAFRETFGSTCHGAGRVLSRAQAVKASRGRSIPKEMKERGVIVMAAGKGTLSEEIPEA
                     YKNLDEVVNVVHEAGLSRKVARLRAVGCIKG"
BASE COUNT         1128 a         1359 c         1446 g          970 t
ORIGIN      
        1 gtccggctca acgacgagtc ggggatcacg atcctcctcg tgacccacga gcccgacatc
       61 gcggaataca gcaaacgcat catccgtttt cttgacggga ggattattag cgacgagaag
      121 aaggtagaaa aatcatgatc agcatccctt caacattcaa gatttctttc cgggccctcc
      181 gggtgaataa gatgcgttcc ggcctgacga tgctggggat cataatcggg gtgggtgccg
      241 tgatcacgat ggtcgccatc ggcacgggcg caagcctgag gatatcccag cagatcgcga
      301 gcgtgggaag caacctaatc gtcatcctgc ccgggagcac gacctcgggc ggcatccgca
      361 tgggcctcgg cagccagcag acgttgacga agggcgacgc cgacgccatc ttgaaggaat
      421 gtccggccgt cgccgaggtg gctcctatca tgaacggcgt cgcccaggtg gtctacggga
      481 acatgaactg gtccacgggg gtgatcggaa ccacgccgag catcctggag gtccgcgact
      541 ggccgctctt ttcggggagg aacttcacgg agcaggacgt ccgcagcgcc gcaaaggtct
      601 gccttctcgg ccagacggtg gtagacaacc tttttggggc ggcggacccc gtcggccaga
      661 tcgtccggat caagaagctc ccctttaccg tcgtcggcgt cctggacagg aaaggtcaat
      721 ctcaacacgg ccaggaccag gacgacacga tcttcatccc cgtcactacc gcccagaaaa
      781 agatcttcgg gacggccttc cccggtatgg tcaggaccat catggtcaag gcccggagca
      841 cggaggacct ggacgccgcc gaaagacaga tcacggcact cctcaggcag aggcatcgta
      901 tcggccaaaa ggcggacaac gatttcagca tcagaaatct tacccagatg atgcaggtcg
      961 ccgagcagtc cacccgggtg atgacaatgc tcctcggggc gatcgcttcc gtttctctcc
     1021 tcgtcggcgg catcggcatc atgaacatca tgctggtctc ggtcacagag aggacgcggg
     1081 agatcggcat ccgcatggcc gtgggggcca agacatggga catccgcctc caattcatca
     1141 tcgaggccct gtctctctcc ctgattgggg gtttcgtggg gatcgcagcg ggcgtcatca
     1201 cggccgagat gatctccttc tttacgggct ggtcgacggt cgtatcgccc ctcgctgtcc
     1261 tcacggcctt cgggttctcc ggcctcgtgg gcatcttttt cggcttctac ccggcctaca
     1321 aggcatccct cttgaatcct atcgaggccc tccactacga atgagtcccc atggggttgc
     1381 cggtgggccc agtttttttg ccctcctctg agataccgcc ggtaaacccg tcctacccac
     1441 ccgttccggc ctccagccca tcttgacaac cctaattttg tgaatacatt atcggcgaaa
     1501 atcagattca gccagtataa acacggcaaa agagattcaa acgataggaa aggaggtata
     1561 gttatggttt ggccatcggt agtaagatac ggcaggatgg tggatccctt cgacgagatc
     1621 cagaggcttc agcaggaaat gaaccgtctc ttttccggtt tcggcccggc cgttgtttac
     1681 gagtttccgc cgattaacgc atgggtcagc gaagacgatg cggtcctcac ggcggaactt
     1741 cccggcatcg acccggagaa gctggatatt tccgtggcag gagataccct cactctgagc
     1801 ggcgaaaggg caccggcaag cctcggtgaa ggggagactt atcacaggca ggaacgaaac
     1861 ttcggtaagt ttgctcgaac gttgaggctt cccttccagg tcgacccgac caaggtagag
     1921 gcaaaatacg aaaaagggat actcaggatt accctcccga ggtcggagca ggacaaaccc
     1981 aagaagatag ccataaagac aaactaaaaa gggggtgtag cgtactatgg caagggaaaa
     2041 gactcaggcg aggatgcctg ttgaagcgga aaggacgaag gaaaagaggg tctttgtgcc
     2101 ccgggtagac atttttgaaa caaaagacgg tatcgtcatc gccgccgata tgcccggcgt
     2161 cgacgataag tccatcgatg taacgctcga gaagaatatc ttgacgatca aaggacgggt
     2221 tgaagcaaaa cctgtcgaag gacacagttt ggcctacgca gagtacgatg agggcgatta
     2281 cgaacgttta ttcaccctaa ccgatgaggt tgaccgagaa aaaatcgagg cgaaggtgaa
     2341 aaacggggtt ctggaagtga tacttcccaa ggccgaaccg gcgaaggcga aaaagatcgc
     2401 catcaaagca gaatgacttt ctaaccgaat agaaaacgac gaaagggggt aggaaatatg
     2461 cctatcagga atttgttacc gtccgtttgg cggcgcagcg aggttcctgt cctgcgggaa
     2521 gagggagatc ctttctctgc cttgcgtcgc gagatgaaca gaatattcga cgatttttac
     2581 cgaagctttg acctcgctcc cttcgcgggt gccgaggaac gcctagggtt tttcacaccc
     2641 gccgttgacg tggtcgaagg gaaaaaggac ataacggtaa aagtggaact tcccggcctg
     2701 gatgaaaagg atgtggaggt gctcctctcg gacgacaccc tcacgataag gggtgaaaag
     2761 aagcaggaaa aagaagagag caccggagat tactatcgta tggaaaggac ttacggcagg
     2821 ttcgagaggg tcatcccgct gccggaaaac gtagacccgg ccggtatcga ggcgaagttc
     2881 aagaagggcg tcctcagcat ccagatgcct aaacgggccg aaatcgagga caaggcgaag
     2941 aagatcgaga taaaagcgga gtgatgcgga aaacccgcag gcgtgaagtc ccggcccttc
     3001 ccgtgcacga ctttctgctt ccgtcgtgcc ccggaggggc ttgctgttat tggtagtcta
     3061 ggcggaataa atttcttttt ccttgacagt tggtgtcggc ctgttatttt tgtgaaaatg
     3121 tttgcaggtt gctgattctt tcttttttga aaccggcctc gtatcctgaa ttctgtggcc
     3181 cttccggtgg gtttatgcta tgaatccgcg ggagagggat gcaaaaggcg ccgactgagg
     3241 ttcacgatga ggacaacgct tcgctctctc tgcatcctga gaatcctctg ccgtgccgac
     3301 ggtccggtgg aagaatcctt catccagagc gggctctttc tcctccagga agccatggga
     3361 gaaaccctgg attgcaattt ctacctggac atgcaaggtc cccgctcgag ggaggtcctc
     3421 gagaccctca tcaccctgga aagggaaggt aaggtggcta tcggcggttc accgcggggg
     3481 ctcctggtcg aggccactga aaagggaaaa cagttcatcc accagggggg tgattggggc
     3541 gtcttcttcg acgtcccccc tgtaagggta ccggaggccc agatcgacgc cgtcctttct
     3601 ctcagggaaa aggaggcgcc actcgagatg gccgccctgg ggacggccct gttcctggcg
     3661 ctgtccgccg caagcccggg taatatcctc gaggaattgg ataaggcaaa gtctgagggg
     3721 aggctcgccg ccgatctcga cgaccggtcg gtggtggagg ggtttcggcg tcttcggagg
     3781 tacggcctcg ggccggcaag aaagggcgag agaacaaagc caaggagatg aaaaggagac
     3841 cgaaagatga agcgttacgg ctggctggct gtcgggttgt gtctgctgat ggttttttcc
     3901 gggtgcggct ataagtcaca aaaacagggc gcgatgatca acgaggcaag cgtggacaag
     3961 gtgatcgtcg acggacagac gaccaagcag gaagtcgtcc tggaattcgg ccctcccacg
     4021 aagaccatgg agggcgagag gatgttcttc tacacctgga cagagacgag taagtcgagc
     4081 atcccgcttt acggcgggga gacctcggtg accaaggacc tgatcatcct cttcgatgac
     4141 aacggcgtag tgaagagtca caaaatcacc aaaaccgcgc gtgaatccaa ggccggcttc
     4201 ggcgggcaga aggaaaagta gccctcagcc ctgcaaaggg ctggcttaga gcacctccca
     4261 ttcgccaagc tccctgggac agtcggtatc cttacggctg tcccagtcga gccatccagt
     4321 gcaacgcagg aagacgtcct ttgcggcttt atccgccgtt tcataggtgc cgagccacat
     4381 accgccgatg cataattcgt aacaacccga gaccgtcggg tcgctcccga tcgtgaatgt
     4441 ccccgccttc gacctgaata tgtacatttt gccacccggt tttaagattg ataaagcttt
     4501 tcagcccttg atacagccga cggcccgcag cctggccacc ttacgtgata gacccgcctc
     4561 gtgaaccacg ttcaccacct cgtccaagtt cttgtaggcc tcaggaatct cttcggagag
     4621 cgtacctttg ccggcggcca tcacgatgac ccccctttcc ttcatctctt tcgggatgga
     4681 cctcccccga cttgccttca cggcctgggc cctgctcaac accctgccgg ccccgtgaca
     4741 ggtgctgccg aaggtctccc gaaaggcctt ctccgtcccc gcaaggacat aggagccgac
     4801 ccccatgtcg cccggtatca gcaccggctg tccgtggcgg cgatagacgg ccggcaccgc
     4861 gggatgtccc ggggggaagg agcgcgtcgc ccccttgcgg tgg
//
LOCUS       DAOTMX010000107         4876 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_14327_length_4876_cov_3.783862, whole genome shotgun sequence.
ACCESSION   DAOTMX010000107 DAOTMX010000000
VERSION     DAOTMX010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4876
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14327_length_4876_cov_3.783862"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="P5573_04200"
     CDS             <1..498
                     /locus_tag="P5573_04200"
                     /inference="COORDINATES: protein motif:HMM:NF024878.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HRT27294.1"
                     /translation="LSAFKGMFPVLQDVAYQHHERENGQGYPQGLRGSEINELAKIIG
                     LLDSYEAMTHSRPYRKALGPSFSAKELLKSKHAAYSPNVIKAFLREISLYPVGSYVRL
                     NNRAVAEVVETNQNHPLRPTVRILFDGEGNEVVDDVVIRLEHNPLFFIVEGLAKEEFL
                     PAIGR"
     gene            complement(495..1298)
                     /locus_tag="P5573_04205"
     CDS             complement(495..1298)
                     /locus_tag="P5573_04205"
                     /inference="COORDINATES: protein motif:HMM:NF017437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRT27295.1"
                     /translation="MYLEYWGLEEFPFENVPNPKFTYYSAEFEEALIRLLYAVERKKG
                     TVLLTGEVGCGKTTLSRVFIQRLSENRSDISLITNPSLDPYDFLKEILYQFGLNPGPI
                     SSKSDLLSLLNMRMLENVRADRNSILIIDEAQVISKETFEEVRLLLNFQMNDRFLLTL
                     ILIGQPELRDIIREIKQLDQRIAIKYHLSPLSAGDSARYILTRLKVAGASRKIFTASA
                     FDEIHRHSLGIPRKINNICDMSLMIGFVAKAKVIDTDVVQNAIKDLYQS"
     gene            complement(1289..1744)
                     /locus_tag="P5573_04210"
     CDS             complement(1289..1744)
                     /locus_tag="P5573_04210"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27296.1"
                     /translation="MKILFVCHANIARSFIAERILKGKLKARGRGDVVVTSAGLYDMK
                     NSEADPLAVGLLLKNGFDASAHRSRILTEDMINEADRVIVMEEAQRQKLVERFPEQED
                     KVCLLKPFSPSFNPKSAPDIRDCHNRSAYHYRLCFAEIHEAIEGLIECI"
     gene            1969..4260
                     /locus_tag="P5573_04215"
     CDS             1969..4260
                     /locus_tag="P5573_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HRT27297.1"
                     /translation="MSAELKKEEVFELEEQLALRNKLLNITNRIHAAQNVKQILVDLK
                     DGILSLMNAYAITIYVADDIKREVYSMVLSGTKLQEIRVPINNSSIAGYVANTKKAVN
                     ISDAYDEEELKHIDAQLCFDSSWDKKSGFRTKQVLAVPIVYEQKLMGVIQIINRKGGG
                     SFSDEERSFLEEIAGVLEIALYNQQRIARRRKTRFDLLLNRGLISEQELENSWQEARK
                     SGESMEEFLMKKYRISKEDLGQSLEDFYRAKFVEFDEKQPVPEGLLTNLRQEYLRREL
                     WVPLDKVDGKVRVLVDDPNNILKRDTIESLLKTKAIEYCVGLKDDILKYINQFYRPSG
                     SAEESIAEMLGRLEITEEKEEEEEEQVSEADSVIMQLVNRIINDANARRASDIHIEPN
                     VQRKTVGVRFRVDGDCVQYMTLPYSYRAATVSRLKIMSNLDITERRLPQDGKIRFKRP
                     GGDEIELRVATIPTQGGVEDVVMRILAKSETMTLEQMGMSPRNYSEFLKILAKPYGIV
                     LVVGPTGSGKTTTLHAALHHINTPDTKIWTAEDPVEITQEGLRQVQVNPKIGFDFSAA
                     MRSFLRADPDVIMVGEMRDFETAKTGVEASLTGHLVFSTLHTNSAPETVVRLLDMGID
                     PFNFADSLLGILAQRLVRTLCKNCKERYHPEEVEFEEIVHHYGPELFKELSIEYNNDF
                     FLYRPKGCSQCNNTGYRGRMGVHELLVASDDIKRLVARKESVEKLRDTAIAEGMRTLL
                     QDGIWKVLGGYTDLKQVLSVCMK"
     gene            complement(4265..>4876)
                     /locus_tag="P5573_04220"
     CDS             complement(4265..>4876)
                     /locus_tag="P5573_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HRT27298.1"
                     /translation="SRGESTFMVEMNEVASILNNATPRSLILLDEVGRGTSTFDGLSI
                     AWAVAEYIHDNPQIGARTLFATHYHQLTDLALTKEGVKNLSVAVKEWGDRIIFLRKIV
                     EGGSSRSYGIQVAGLAGVPPEVIARAREILENLERGEFDDVGMPRIARPRKATQKARP
                     VQPSLFAGEETVILDELRRLDIMNTTPLEALGMIHRWKKIVEK"
BASE COUNT         1224 a         1174 c         1358 g         1120 t
ORIGIN      
        1 ttgagtgcct ttaaaggtat gttccccgtt ttgcaagatg tggcctatca acaccatgaa
       61 agggaaaacg gtcaggggta tccgcaaggc cttcggggaa gcgaaataaa cgagctggcg
      121 aaaataatcg gcctgttaga cagctatgaa gccatgacgc acagtaggcc gtacagaaag
      181 gcgctggggc cgtcgttttc ggcgaaggaa ttgctcaagt caaaacacgc ggcgtattcg
      241 ccgaatgtga taaaggcctt tcttagagaa atttcccttt atcctgtagg aagttacgta
      301 agactgaata atcgggccgt cgccgaggtc gtagaaacca atcaaaatca tcccttgagg
      361 ccgacggtga ggatactctt cgacggtgaa gggaatgagg ttgtcgatga cgtcgttatc
      421 aggcttgagc acaacccact ttttttcata gtagaagggt tggcgaaaga ggagttccta
      481 ccggcaatcg gccgctaact ctggtaaagg tccttgatcg cgttctggac aacgtcggtg
      541 tcgatcacct tggccttggc gacgaaaccg atcatcaaac tcatgtcaca tatgttgttg
      601 atttttcggg gaataccgag ggaatgccga tggatttcgt cgaatgcgct ggcggtgaag
      661 atttttcgac tcgcgccggc gaccttgagc ctggtcagga tgtagcgggc cgagtcgccg
      721 gcactcaggg gcgaaaggtg gtacttgatc gcgattcttt ggtcaagttg ctttatttct
      781 cggatgatgt ccctcaattc cggttgaccg atcaagatga gggtgagaag gaaacggtcg
      841 ttcatctgaa aattcaagag tagacgcacc tcttcgaagg tctctttgga aataacctgg
      901 gcctcgtcga tgatcagtat ggagttcctg tcggccctca cgttttcgag catccgcatg
      961 ttcaacaagc tcagaaggtc cgatttcgat gaaataggac ccggattaag gccgaactgg
     1021 tagaggatct ccttgagaaa atcgtagggg tcgaggcttg ggttcgtgat gagactgata
     1081 tcggagcgat tctcggatag gcgctggata aacacgcggc tcagtgtcgt cttgccgcaa
     1141 cccacctcgc cggtcagaag gactgtgccc tttttccttt ccacggcgta gaggagcctt
     1201 atcagggcct cctcgaactc tgccgaatag tacgtgaatt tcgggttagg gacgttctcg
     1261 aagggaaatt cttccaggcc ccagtattct aaatacattc tatcaaccct tcgatggctt
     1321 cgtgtatctc agcgaagcaa agcctgtaat ggtacgccga cctgttatgg cagtctctga
     1381 tatcaggggc ggattttggg ttgaaggacg gcgagaacgg tttgagaaga cagaccttgt
     1441 cctcctgttc cgggaaccgt tcgacgagtt tttgacgctg tgcctcttcc atcacgatga
     1501 ccctgtctgc ctcgttgatc atgtcctctg taaggattcg tgaccgatgt gccgatgcat
     1561 cgaaaccgtt tttcaggagc agaccgacag caagcgggtc agcctcggaa tttttcatgt
     1621 cgtatagtcc ggccgaagtg acgactacat ccccccgccc cctcgccttt aacttgcctt
     1681 taaggatcct ctctgcgatg aaactacggg caatgtttgc atgacagacg aacaagattt
     1741 tcatcgtcgg ctaatataaa accaacttcc gagccggcga aggggagaac taatcgccgg
     1801 caagcagact atagatatcc actatttttg tggtaatata tactcgctat gactcatatt
     1861 caagttttta ttgattataa aaggattctt tgcttgcatg gagttgacct gccggtccgg
     1921 cgatgcataa gggccggaag agatgaatcg gttagaggat gagtatcgat gtcagccgag
     1981 ttaaaaaaag aagaggtttt cgagttagaa gaacagcttg cgctgcgcaa caaacttctg
     2041 aacatcacta acagaataca cgcagctcag aatgtcaagc aaatcctcgt ggacctcaaa
     2101 gacggtatcc taagcctgat gaacgcctat gccatcacca tctatgtcgc cgacgacata
     2161 aagagagaag tgtactccat ggtgctctcg gggactaaat tgcaagagat cagggtcccg
     2221 atcaacaatt cgagcatcgc cggttatgtg gcgaacacga agaaagcagt gaacatttcc
     2281 gacgcctacg acgaagagga actgaagcac atcgatgccc agctatgctt cgacagcagc
     2341 tgggacaaga aatctggttt taggacaaag caggtgttgg ctgttccgat cgtttatgag
     2401 caaaaactga tgggggtaat tcagatcatc aaccgcaaag gcggtgggtc gttctcagac
     2461 gaagagaggt cttttctcga agaaatcgcc ggggttttgg aaatagcgct gtacaaccag
     2521 cagcgtatcg cccgcaggag gaaaacgcgc ttcgatttgc tcttgaacag gggattgatt
     2581 tccgagcaag agctcgaaaa ttcgtggcag gaggcacgaa aatccgggga atctatggaa
     2641 gaatttctga tgaagaaata ccggatttcc aaggaagatt tggggcagtc ccttgaagat
     2701 ttttacaggg ccaagtttgt cgaattcgac gaaaaacaac cggtcccgga aggactttta
     2761 acgaaccttc ggcaggaata cctgcggagg gagctatggg taccgctcga taaggttgac
     2821 ggcaaggtca gggtgttggt ggacgacccg aacaacatct tgaaaaggga cacgatagag
     2881 agtctcttga agacgaaagc catagagtac tgcgtcggcc tgaaggacga catcttaaag
     2941 tacataaacc agttttatcg cccttccggt tctgcggaag aatcgattgc cgagatgctg
     3001 ggacgtctcg agatcaccga ggaaaaagaa gaagaggaag aagagcaggt gtccgaggcg
     3061 gatagcgtca tcatgcagct cgtgaacaga atcatcaacg acgcaaacgc caggagggcg
     3121 tcggatatcc acatcgagcc gaatgtgcaa agaaagacgg tgggtgtcag gttccgggtc
     3181 gacggcgatt gcgtccagta catgactctt ccctacagtt atcgtgctgc caccgtgtcg
     3241 aggctcaaga tcatgtcgaa cctcgacatc acggagcgaa ggttgcctca ggacgggaag
     3301 atcaggttca agcgaccggg tggcgacgag atagaattgc gagtggcgac catccccacc
     3361 caggggggtg tggaggatgt ggtgatgagg attctcgcaa agagtgagac catgaccctc
     3421 gagcagatgg ggatgtcacc gcggaactac agcgaatttt tgaaaattct tgcgaaaccc
     3481 tatggaatag tcctggtcgt cggtcccacc ggttcgggaa agacgacgac cctccacgca
     3541 gcactccatc acatcaatac gccggacacg aagatctgga ctgccgagga tccggtcgaa
     3601 atcacccagg aaggactacg acaggtacag gtaaacccta aaatcggctt cgatttcagc
     3661 gccgccatgc gctcttttct gagagcagac ccggacgtca tcatggtcgg tgagatgagg
     3721 gatttcgaga cagccaagac aggcgtcgag gcctctctca cgggccacct tgtcttcagt
     3781 actctccata ccaacagcgc cccggagacg gtcgttcgct tgctcgacat gggcatagac
     3841 cccttcaatt ttgccgattc gctgctcggg atactcgccc agcggctggt tcgaacgctt
     3901 tgcaagaact gcaaggaacg ctaccatccg gaagaggttg agtttgagga gatagtccat
     3961 cattacgggc cggaactgtt caaggaacta agcatcgaat acaacaacga ttttttcctt
     4021 tacaggccga agggatgcag ccagtgcaac aatacggggt accgaggccg gatgggcgtg
     4081 cacgagcttc ttgtggcgag tgacgacata aagagactcg tggccaggaa agaatcggtg
     4141 gagaagctca gagacaccgc catagcagaa gggatgagga ctcttcttca ggacggcatc
     4201 tggaaagtgc tgggcggtta cactgacttg aaacaggtct tgagtgtctg tatgaaataa
     4261 ccgtttattt ttcaacgatt tttttccatc gatggatcat gccgagggcc tctaaggggg
     4321 tcgtattcat gatgtcgagt cttctcaatt cgtcgaggat gacggtctct tcgcctgcaa
     4381 aaaggctcgg ttgaacgggc cttgcctttt gtgtggcctt tcggggacgg gcgattcgcg
     4441 gcataccgac atcatcgaac tccccccttt caaggttttc cagtatctct cgggccctgg
     4501 cgatgacctc aggcgggacg ccggccagtc ccgccacttg aataccgtaa ctacgactgg
     4561 aacctccctc cacaatcttc cgcaagaaga tgatccggtc gccccattct ttgacggcga
     4621 cgctgagatt cttgacgcct tccttggtaa gggcaaggtc tgtgagctgg tgatagtgag
     4681 ttgcaaacag ggtccgggcg ccgatttgcg ggttgtcgtg gatgtattct gccactgccc
     4741 aggctatact gagcccgtcg aaagtgctgg tgcctcgtcc gacctcgtcg aggagtatca
     4801 ggctcctggg tgttgcatta ttcaaaatgg aggcgacttc gttcatctcc accatgaaag
     4861 tgctttcccc ccggga
//
LOCUS       DAOTMX010000108         4871 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_14349_length_4871_cov_3.792151, whole genome shotgun sequence.
ACCESSION   DAOTMX010000108 DAOTMX010000000
VERSION     DAOTMX010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4871
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14349_length_4871_cov_3.792151"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1913)
                     /locus_tag="P5573_04225"
     CDS             complement(<1..1913)
                     /locus_tag="P5573_04225"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HRT27299.1"
                     /translation="MAQYDLDLREYWRIVKKRKFIVLFTTIALGFFSFIFAVIGSPTP
                     LYKASAAVKFERFSPASGLFLFSYSPGDNMQTQMAIIKSYPVIQATAQKLGLIPQDLT
                     AEEIRNNPEYLGIINKLKGMVETEQEAQSSIINVHVTADDPRFAQRLANTLASVYKEQ
                     RTAELNKRTTESKKFVEEQLKIAKERLKNSEEAVRDYREKQKLISVDSTAGSLMAQLG
                     SYKTAYEKAVVDYEKVQNALSQLNRAETRPFTSKTSFYLEEAPPVYKSMNDRLIQLLM
                     DRDSLLITYTENHPQVVEIKQRIHEVVQNMKAQLEAYSKSLRENIQFLKSKIDEAERQ
                     VKSLPEKGLELTRLERNVKVDTDVYSLLEKSYQEALIKEAEKVEDVQIIRPALEPTSP
                     VNPPKTTATAGIGTLLGLVLGLVFAFMLETFDTSIGTIEEVEKFLGVPVLGIIPQINL
                     KEIKSLLSEKAAGEVDEETAERASRLITHFVPKSTVAESYRALRTNLNFACRDRAIKA
                     IVFTSATPREGKTTTIANLAIAIGQSGQRVLLAEMDLRNPIIAHMFGIEKTPGLTDVI
                     LGNLGWDSAVKTIADMMMGRFSVDELLETPGIDNISIIPSGTVPPNPAEFMNSKSIGD
                     LIEWAKSNYDVIL"
     gene            complement(1947..3131)
                     /locus_tag="P5573_04230"
     CDS             complement(1947..3131)
                     /locus_tag="P5573_04230"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HRT27300.1"
                     /translation="MSGTKNRLLVVIVATFCLTACYGGFKATALPVHQYLDTEEVHAV
                     QKEKVMAYTLEEIKRMAQATENSVFTERRGYPEYIVGPGDVLMINFWEGPNPKPYETT
                     VRPDGKISYSFIDDLTVSGLTTSEIREALTRALEKYIRVPRIEVTVKEFKSKSALLSG
                     QINVLQQGISGPGKYNLKGKMTVLDLIILAGGPIVGRVPPSKSTSGYAGYGAVSTGDA
                     DMKHVELVRKGKKYTLNLYDAMFKGDMSQNVVLDDGDLVTVPELPIFGERIYVFGEVN
                     MEGVYRLKDAADFLSAISISNGLTRTAIKEDIRIIRGYKERNRKPLILSVNYWDIVKR
                     GDLSQNVLLMDGDVVYVPRTAIGDVNEFIINTTPLLDYLFYPSRYRDAYMNINWMRLN
                     NF"
     gene            complement(3246..4610)
                     /locus_tag="P5573_04235"
     CDS             complement(3246..4610)
                     /locus_tag="P5573_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HRT27301.1"
                     /translation="MNPKVFREYDVRGVVGEDLNDDFVYELGRAVGTYAARRGVKTMT
                     LGRDCRLSSESYQQAVKRGLMSTGLEVIDIGLCATPMLYYSIRRLQADGGVMVTGSHN
                     PPEFNGFKICVGPDTIYGEEIQDVRRLMEKEEYLFGKGSSWQRDITPDYQDYLVRSVE
                     VKPGMSVALDAGNGVGGYFALPILEKFGCKVMPLFCEVDGRFPNHFPDPTVPENIESL
                     VTLVKEQGADVGVAFDGDADRIGVVTDKGDILYGDEILLLFARAILSERPGAAVIGEV
                     KCSQKLFDDIERHGGRAIMWKSGHSLIKSKMKEEKALLAGEMSGHMFFADRYYGYDDA
                     IYAMVRLLEILSKTERPLSEILADVPKTYSTPEIRRDCPDDIKFLVVDRLKDHFKNEY
                     PVIDIDGVRIPFPDGWGLVRASNTQPALVLRFEAQTPERLLAVESTVEEALAKILREF
                     GVQH"
BASE COUNT          983 a         1271 c         1336 g         1281 t
ORIGIN      
        1 atcaagatga cgtcgtaatt ggactttgcc cattcgatga ggtcgcctat ggatttggaa
       61 ttcatgaact cggccgggtt gggcgggacc gtgccggacg ggatgatgct gatgttgtct
      121 atgccggggg tttcgagcaa ttcgtcgacg ctgaatctgc ccatcatcat gtctgctata
      181 gtcttgaccg cgctatccca tccgaggttt cccagtatga cgtcggtcag gccgggagtc
      241 ttttctatcc caaacatgtg ggcgatgatg ggatttctca ggtccatctc ggcaagaagg
      301 acccgttggc cggattggcc tattgcgatc gccaggttgg ctatcgtcgt tgttttgcct
      361 tctcgcggcg ttgcgcttgt gaagacgatc gccttgattg cgcggtcacg acaggcgaaa
      421 ttcaggtttg tcctcaaggc ccggtagctt tccgcgacgg tcgatttggg cacaaaatgg
      481 gttatcaggc gtgacgcccg ctcggccgtt tcttcatcta cctcgcccgc agctttttcc
      541 gagagtaatg atttgatttc tttgaggttt atttgaggaa tgatgcccag aaccgggacg
      601 ccgaggaatt tttccacctc ctcgatagtg ccgatggatg tatcgaaagt ctcgagcata
      661 aacgcgaaga ccaggccgag tacgagaccc aggagcgtgc cgattcccgc agttgccgtt
      721 gtctttggcg ggttcacggg gcttgtcggt tcgagggcgg gcctgatgat ctggacgtcc
      781 tcgaccttct ccgcctcttt gatgagtgct tcctgatagc tcttctcgag caatgagtag
      841 acgtccgtat cgacctttac gtttctttcc agccgtgtca gttcaaggcc cttttcggga
      901 aggctcttga cctgcctttc cgcctcgtcg atcttgctct tcagaaactg gatgttttcc
      961 cggagggatt tgctgtaggc ctccagttga gccttcatgt tctggacgac ttcgtggata
     1021 cgctgcttga tttccacgac ctgggggtga ttttcggtat aggtgatgag gagagaatcc
     1081 ctgtccatca ggagttgaat cagtctgtcg ttcatagatt tatagacggg cggggcttcc
     1141 tcgaggtaaa agctggtctt ggacgtgaag ggccgggtct cagccctgtt cagttgactc
     1201 aacgcgttct ggaccttttc gtaatctaca accgcctttt cgtaggctgt tttgtatgac
     1261 ccgagttggg ccatcaaaga cccggcggtg gagtctacgg agatgagctt ctgcttttcc
     1321 cggtagtcac ggacggcttc ctcggaattc ttcagccttt ccttggctat cttgagttgt
     1381 tcttcgacga atttttttga ttccgtagtt cgtttattga gttccgccgt tctttgttct
     1441 ttgtagacag aggccagggt gttcgccagg cgctgcgcga agcgggggtc atcggcagtg
     1501 acgtggacgt ttatgatcga gctctgggct tcctgctccg tctcgaccat gccttttaac
     1561 ttgtttatga tgccgaggta ctcggggttg tttctgatct cctcggccgt gaggtcctgc
     1621 gggatcaacc cgagcttttg ggctgtggcc tgtatcaccg ggtaactttt gatgatggcc
     1681 atctgggttt gcatattgtc tcccggagag taggagaaca aaaagaggcc ggaggcaggg
     1741 ctgaacctct cgaattttac ggcggcgctc gctttgtaga ggggtgtagg tgacccgatg
     1801 acggcaaaga tgaagctgaa gaatcccaag gcgattgtcg tgaagaggac gataaacttt
     1861 ctttttttga cgattcgcca gtattctctc aggtcaaggt cgtattgcgc cattacgtga
     1921 agttcatccg gggtagatgc ggaggcttag aagttgttga gcctcatcca gttaatgttc
     1981 atgtaggcat ctctataccg tgaggggtag aagaggtaat cgagaagggg tgtcgtattg
     2041 atgatgaatt cgttgacgtc gccgatcgcc gtcctcggta cgtagaccac gtcgccgtcc
     2101 atcaacagga cgttctgact caggtcgccc ctcttcacga tatcccagta gttgacggaa
     2161 aggatgagcg gtttgcggtt cctctccttg taacccctta tgatacgtat gtcttctttg
     2221 atcgccgttc gcgtaaggcc gttcgagatt gagatcgccg aaagaaaatc ggcggcgtct
     2281 ttcagccggt agacgccctc catgttgacc tctccgaaaa catagatacg ttcgccgaag
     2341 atgggcagct cgggaacagt cacgaggtcc ccgtcatcca ggacgacgtt ctgggacatg
     2401 tcgcccttga acatggcgtc gtagaggttg agggtatatt tcttgccttt cctgactaac
     2461 tcgacatgtt tcatgtccgc gtcgccggtg gaaacagctc cgtatccggc atacccagat
     2521 gttgactttg acggaggaac acggccgacg atgggtccgc cggcgaggat gatcaagtcg
     2581 aggaccgtca ttttcccttt aaggttgtac ttgccggggc ctgaaatccc ctgctggagt
     2641 acgttgatct gccccgaaag cagggcggac ttggacttga actcctttac cgtgacttcg
     2701 atgcgaggta cgcggatgta cttttccagg gcccttgtca aggcctcgcg tatctccgag
     2761 gtggtgaggc cgctaacggt taggtcgtcg atgaacgagt aagatatctt accgtcgggc
     2821 ctcaccgttg tctcgtaagg ctttgggtta ggtccttccc aaaagttgat cataagaaca
     2881 tcgcccggcc cgacgatgta ctcggggtaa ccgcgccgct ctgtaaacac ggagttctcc
     2941 gtggcctggg ccatcctctt gatttcctcc aaggtatagg ccatcacctt ttctttttgt
     3001 acggcatgta cctcttcggt gtcgaggtat tgatggacag gcaaggctgt ggccttgaaa
     3061 ccgccgtagc aggcggtaag gcaaaatgtg gcaacgatca cgacgaggag cctgttcttt
     3121 gtacctgaca tgataacctc tacgtgaaat ctaccattga taacgtttca attacaataa
     3181 aaactttcat aatgatatcg gccctaaagg ggatttcatc cacctgtcgg ccgcccatca
     3241 accggtcaat gctgcactcc gaactcccgt aagattttcg ccagggcctc ttcgacggtc
     3301 gactctactg caaggaggcg ctcaggggtc tgggcctcga aacgcaggac cagtgccggc
     3361 tgtgtatttg atgcccgtac cagaccccac ccgtccggaa aaggtatgcg aaccccgtcg
     3421 atatcgatta cagggtactc atttttgaag tgatccttca atctgtcgac gaccaagaat
     3481 ttgatgtcgt ccgggcaatc acgacggatt tcgggggtgc tgtatgtctt cggcacgtcg
     3541 gccaggatct cgctcaacgg cctctccgtt ttggacagta tctcgagcag gcgtaccatt
     3601 gcgtatatgg catcatcata gccgtagtac cggtcggcaa agaacatatg gccgctcatt
     3661 tctcctgcaa ggagggcctt ttcttccttc atcttacttt tgatcaaaga atggccggac
     3721 ttccacatga tcgcccgacc tccatgacgt tctatgtcgt caaaaagttt ttgcgagcat
     3781 ttgacctcgc cgatgaccgc ggcgcccggt ctttcgctca ggatggcacg ggcgaacagc
     3841 aacagtattt catcgccgta aaggatatca cccttgtctg tgactacgcc gatgcggtcc
     3901 gcgtcaccgt cgaaggcgac gccgacgtcg gcgccctgct ctttaacgag tgtgacgagg
     3961 ctctctatgt tctcgggaac ggtggggtcg gggaagtgat tgggaaacct gccgtcaact
     4021 tcgcagaata ggggcatgac cttgcagcca aatttctcaa ggattgggag ggcaaaataa
     4081 cctccgacgc cgtttcccgc gtcgagagcg acgctcatgc cgggtttaac ttctacgctt
     4141 cggacaagat aatcctggta atcaggggta atgtcccgct gccaagagga acctttcccg
     4201 aagaggtatt cttctttttc catcaatctc ctcacatcct ggatttcctc gccgtagata
     4261 gtatcaggcc cgacgcagat cttgaatccg ttgaattcag gcgggttgtg gcttccggtg
     4321 accatgacgc cgccgtccgc ctgtaatctt ctgatagagt agtacagcat gggtgtggcg
     4381 caaaggccga tatcgatcac ctccagccct gtagacatca accctcgttt tacagcctgt
     4441 tggtaggact ccgaacttag ccggcaatca cggccgaggg tcatagtctt tacaccgcgt
     4501 cgggccgcgt acgttccgac cgccctgccc agttcataga caaagtcgtc gttcaaatcc
     4561 tctccgacga caccccggac gtcgtattcc ctgaatactt taggattcat gaagggcccc
     4621 tttccttatt gtgcaggaaa tacttaacat atccgggagt caatcacaaa aaaattctgt
     4681 tttttccctc gccgaggccg catagttacg ttgggggcgg aaccggagcg atgcgccctt
     4741 aatcccccag gcccctcgga gcggcacatt tttccttgaa gtaatgccga ctttaagata
     4801 tacggtagcg acagatgggc ggttaactca gcgggagagt gctaccttca cacggtagaa
     4861 gtcactggtt c
//
LOCUS       DAOTMX010000109         4870 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_14353_length_4870_cov_3.835722, whole genome shotgun sequence.
ACCESSION   DAOTMX010000109 DAOTMX010000000
VERSION     DAOTMX010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14353_length_4870_cov_3.835722"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="P5573_04240"
     CDS             <1..363
                     /locus_tag="P5573_04240"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRT27302.1"
                     /translation="AYNNMGYAYYRKGDYQAALASFDEAFARDPNTLLTPLIEKNRGM
                     VLLAQGRVREAIDHLKKSLEIAPDFPETHYLLAKCHVEMKDLKEAEAEFRLVLKLAPD
                     SELGEKAKESLAAITGSH"
     gene            554..1183
                     /locus_tag="P5573_04245"
     CDS             554..1183
                     /locus_tag="P5573_04245"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HRT27303.1"
                     /translation="MGSKRKRRSEEVRAKKKRKGIRLFLLSVIVAVVLGFLVFLGVSL
                     YDFVGPSSEKGPLPKKENRQVVLYFSDVQERFLVPAKRMIPEATTAQGRAEELVKALI
                     EGPGAGLKELTRTIPEGTRLRGVRVEAEGTAFVDFDKSIVDRHPGGSASEVATVYSLV
                     NTLTQNVPEIKAVKILVEGKPRETLKGHLDLGYPLRFNGELIKEQSPTG"
     gene            1207..3243
                     /gene="fusA"
                     /locus_tag="P5573_04250"
     CDS             1207..3243
                     /gene="fusA"
                     /locus_tag="P5573_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRT27304.1"
                     /translation="MASRTKISRIRNIGIVAHIDAGKTTVTERILFYTGKTHKMGEVH
                     DGEAVMDWMPQEQERGITISSAVTTCHWRNHEINIIDTPGHVDFTIEVERSLRVLDGA
                     VVVFSAVEGVEPQSETVWLQADKYGVPKIAFINKMDRIGADYYNAVAMMKERFNSAPL
                     MVQIPWIKKEEGFGGVIDLIKKKRMTWETDSLGLSFSVSDIPDEAAMDAEIQRDRMIE
                     TLAEVDDVIADAYLEGSEVSENGIREAIRKATIDLKVVPVLCGSALRNTGVQPLLDAV
                     VDFMPSPQDLPPVKGFNPVTREEVSRKADDKEPLTALAFKVMMAEGRKMTYLRVYSGT
                     MEAGQEVYNATRGRTEKVARLLKVHANKRERITDAGAGDIIAAMGLKETSTGDTLCDE
                     KNPIVLEPIEFLEPVISRAIEAKTPGDQERLSEALAKLVDEDPTLRVKYDDETAQTVI
                     SGMGELHLEIVIDRLIREFNVRVNVGKPRVVYRETIEKTVEIEGRFDKELGERKHFGH
                     VKMRLEPSARGSGIEIIDEIQPEDIPVIYHKAIEEGVREATLSGVLSGYPVVDLKVHL
                     TGGTFRDGESSEVGYRIAASTAFKEGCRKASPILLEPIMSLNVLTPSEYMGEVIGDIN
                     VRRGEVKTISHKGPTCEISAKVPLKAMFGYATDLRSATQGRATFTMQFSEYDKV"
     gene            3424..>4870
                     /locus_tag="P5573_04255"
     CDS             3424..>4870
                     /locus_tag="P5573_04255"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRT27305.1"
                     /translation="MRREGTNITSRTQSTFVIDKDKLIRSMYRISALLTDPSSVDEIS
                     WQIAFDEFPYGFRQLSEVSYVDGIMSKILDEVTDNLGFDRGIICLLDEQKENLITRVV
                     KNYDPEEARRAFSVNLNLKKHDSLETRVAKTGQYITLEDSDRDPRLTSTDRKITEFYK
                     RGSTFYAPLKVEDEVMGIIAVWTKEKTRFTPEEIDILLTFANQVSIIIHLTRLFENNS
                     EKIHQLLILQKAVSELNASSAVDDIHEIILKYALKIGQSEKALIYFTDSKQGQCLVHD
                     GQKIVAVHAEEYAKRIDGSIIRQAMETNAIVVRQVAASRPAGTAALFEDYDSEIVFPL
                     RIKNDFKGALYLAKIEGNWPQDRINILDILIKNAGTSYDNAIMHSYLSLEAKSLKSEV
                     EKLKEREDALLGFHDIIGKSEQMQTIFRIIEEVAMHDTNVLIQGESGTGKELFARAIH
                     RQSRRSTKRFVEVNCAAIPETLLESELFGYEQ"
BASE COUNT         1364 a         1135 c         1378 g          993 t
ORIGIN      
        1 gcttacaaca atatgggata tgcttactac agaaaaggcg actaccaggc ggcgctcgcg
       61 agttttgatg aagccttcgc cagagacccg aacacgttgc tcacccctct catcgagaag
      121 aaccgcggga tggtcctgct tgcccagggt cgtgtaaggg aagccatcga ccatctaaag
      181 aaatcacttg aaatcgctcc tgattttcct gaaacccatt acttgctggc taaatgtcat
      241 gttgagatga aagacctcaa agaggcagaa gccgaattcc gtctggtttt gaaacttgca
      301 cccgattcag agcttggcga gaaagcaaaa gagagccttg cggcgatcac tggaagccac
      361 tgaaccgccg actcccctca cgatcagctt gacacccctt ttcctgctga atcatggagc
      421 atcagcttga cagcaagatg aaaatggcct aagttggcgc gtcttttatg ggctactggt
      481 aggatacggt cagaaaaacg gatttaaagc cgccggcaga ttcttcgatt gataacaaga
      541 aagaaagcgg aaaatgggga gtaagagaaa aagacgttcc gaagaggtaa gggcgaaaaa
      601 gaaacgcaaa ggcatccggt tgtttctgct atcagtcatc gtagcggtcg tactcgggtt
      661 tctggtcttt ctcggtgtca gtctgtatga tttcgtcgga ccgtcgagtg aaaagggccc
      721 cttgcccaaa aaggagaaca ggcaggttgt cttgtatttc tctgatgtcc aggaacgttt
      781 tttggtcccc gcaaaaagga tgatccctga ggcgacgact gcccagggac gggccgaaga
      841 attggtcaaa gccctcatag agggaccagg ggccgggctt aaggaattga cgcgcacgat
      901 acccgaaggc acgagattga gaggggtccg ggtcgaggca gagggtacgg cctttgttga
      961 tttcgacaag tctatcgtgg ataggcaccc gggaggaagc gcgagtgaag tggccactgt
     1021 gtattcgctg gtcaacacgc tgacgcagaa tgttccggaa atcaaggccg tcaagatctt
     1081 ggtggagggt aagccgcggg agaccctaaa gggacatctt gatctcgggt atcctttgag
     1141 attcaacgga gagctgatca aagagcagtc gccgactgga tgacttagtc agaaagtgcc
     1201 gggataatgg catcgagaac aaagatctca cgcatcagga acatcggcat agtggcgcac
     1261 atcgacgcgg gaaagacgac tgtaacggaa agaatactct tttataccgg caagacccac
     1321 aagatgggag aggtccatga tggagaagcc gtgatggact ggatgcccca ggagcaggaa
     1381 aggggcataa cgatatcgtc tgcagttacg acatgccact ggcggaatca tgagatcaac
     1441 atcatcgaca cccccggcca cgtcgacttt acgatagagg tggagcggag tctgcgcgtc
     1501 cttgacgggg ccgtcgtagt tttcagtgcc gtcgagggtg tagaaccaca gtcggagacg
     1561 gtatggttgc aagcagataa gtacggcgtc cctaaaatcg ctttcatcaa caagatggac
     1621 cggatagggg ccgattatta caacgccgtg gccatgatga aggaacgatt caactcggcg
     1681 ccgctgatgg tccagatacc ttggattaag aaggaagagg gctttggcgg tgtcatcgac
     1741 ctcattaaaa agaagcgaat gacctgggaa acggatagtc tgggcttgag cttttccgtc
     1801 tccgacatcc ccgatgaggc cgcgatggat gcggaaattc aacgggacag aatgatcgag
     1861 acacttgctg aggtcgacga cgtaatcgca gacgcctatc tcgagggaag tgaggtctct
     1921 gagaatggaa taagagaggc catccgaaag gcgaccatcg acctgaaggt cgtgccggtc
     1981 ctttgcggct ccgccttgag aaacacgggg gttcagcccc tcctggacgc agtcgtcgat
     2041 tttatgccct ctccccagga tctgccgccg gtcaaggggt ttaaccccgt cacgagggaa
     2101 gaagtaagta ggaaggccga cgacaaagaa cccctgacgg cccttgcgtt caaggttatg
     2161 atggccgaag gaaggaaaat gacctacctc cgagtatact cggggacgat ggaagctggg
     2221 caggaagtgt ataatgcgac caggggcagg acggaaaaag ttgcaagact cttgaaggtg
     2281 cacgccaaca aaagagagag gataaccgac gccggtgcgg gggacatcat cgcggcaatg
     2341 ggtttgaaag agacctcgac gggagataca ctgtgcgatg aaaagaatcc gatcgtgctc
     2401 gagcccatcg aattcttaga gccggtgatc agccgagcga tcgaagcaaa gacgccaggt
     2461 gaccaggaaa ggctgtccga agcgctcgcc aaactggtgg atgaggatcc gacgctgagg
     2521 gtgaaatatg acgacgaaac cgcacagaca gtcatatccg gtatggggga actccatctg
     2581 gagatagtca tcgaccggtt gatcagagag ttcaacgtaa gagtcaacgt cggaaagccg
     2641 agggttgtct acagagaaac aatagagaag acggtggaaa ttgaaggccg cttcgacaag
     2701 gaactgggag aaagaaaaca tttcgggcac gtaaaaatga ggctggaacc gtcagcccgc
     2761 ggctccggca tagagatcat cgacgagata cagccggagg acattcccgt catctatcac
     2821 aaggcgatag aagaaggtgt aagggaggcg actctcagcg gtgtactttc cggatacccg
     2881 gttgtcgacc tgaaggtcca tttgacaggc ggaacttttc gtgacggcga gtcctcggaa
     2941 gtaggttacc ggatcgcagc gtcgacggcc ttcaaggaag gatgtcgtaa agcaagtcct
     3001 attctactcg aacctataat gagtcttaac gttttgaccc cgagtgaata catgggagaa
     3061 gtcatagggg acatcaatgt gaggcgcggt gaggtcaaga ccatcagtca caaaggaccg
     3121 acctgtgaaa ttagcgcgaa ggttcctttg aaggcgatgt ttgggtatgc gacggacctc
     3181 aggtccgcta cccagggtag agcgactttt accatgcagt tttcagaata cgataaagta
     3241 tgacgccgtt gcatgatcac ctcaaaacgc ccgctacttt tctccgcaac cggaactgag
     3301 tagtccggaa taagaccgaa tgcaccagat ttaatttctg attttcatcg tgattacccg
     3361 ttggagggtg ttcagtctcg cctcgcggga aaaacgaaat aaaaataaag ggacaaatca
     3421 ttcgtgagaa gagaaggcac gaatattact tccagaacac agtcgacctt cgtgatcgac
     3481 aaggacaagc tcatccgtag catgtacaga atcagtgcat tgctgacaga cccctcctcc
     3541 gttgacgaga tttcatggca gatcgctttc gatgaatttc cctatggttt ccgacaattg
     3601 tcggaagtat cgtatgttga cgggatcatg tcaaaaatcc ttgatgaagt aactgacaac
     3661 ttgggtttcg accgcgggat catctgcctg ctggacgagc agaaagaaaa cttaataaca
     3721 agggtcgtca agaactatga ccccgaggag gccaggcggg cgttctctgt caacttgaat
     3781 ctcaagaaac acgacagcct cgagacgagg gtagcgaaaa ccggtcagta catcaccctt
     3841 gaagactcag atcgcgaccc aagattgacg agcacggacc gcaagatcac ggagttttac
     3901 aagaggggat cgacgttcta tgctcccttg aaggttgagg atgaggtgat ggggatcatc
     3961 gccgtctgga ctaaagagaa aacgagattc acccctgaag aaatagacat cctcctgacc
     4021 tttgccaacc aggtcagtat aataatacat ctgaccaggt tgttcgaaaa caacagcgag
     4081 aagatccacc agctcctcat ccttcagaag gccgtttccg agcttaacgc gagcagcgcc
     4141 gtcgacgaca ttcacgagat catactcaag tatgccctga aaatcggcca atcagagaag
     4201 gctctgatct atttcaccga ttcaaagcag ggtcagtgtc tggtccatga cggccaaaaa
     4261 atagttgccg tccacgccga agaatacgcg aaaaggatag acgggagcat catcagacag
     4321 gcgatggaaa ccaatgccat tgttgttcgt caggttgcgg cgagccgtcc ggcaggaacg
     4381 gccgcgttgt ttgaagatta tgactccgag atcgtctttc ctttgaggat aaagaatgat
     4441 ttcaagggtg ccctttatct ggcaaagata gagggcaact ggccgcagga tcggatcaat
     4501 atactggata tcctgataaa gaacgcggga acgtcttatg ataatgcgat aatgcattct
     4561 tatctgtccc tcgaggcgaa atcattgaag tcggaagtcg aaaaactgaa agagcgagaa
     4621 gacgccctcc tggggttcca cgatattatc ggcaaatcgg agcagatgca gaccatattc
     4681 cgtatcatcg aagaagtagc catgcatgac accaacgtcc ttattcaggg cgagagcggc
     4741 accgggaaag aactctttgc cagggctata cataggcaga gcagaaggag tacgaagcgt
     4801 tttgtggagg tcaattgcgc cgccataccg gaaaccctgc tggagagcga acttttcggt
     4861 tatgagcaag
//
LOCUS       DAOTMX010000110         4788 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_14629_length_4788_cov_4.374815, whole genome shotgun sequence.
ACCESSION   DAOTMX010000110 DAOTMX010000000
VERSION     DAOTMX010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14629_length_4788_cov_4.374815"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            511..957
                     /locus_tag="P5573_04260"
     CDS             511..957
                     /locus_tag="P5573_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27306.1"
                     /translation="MLSRLIPTRELSGIFSVFLQRLQRGQQLSRYRLLGGKYLVAIDG
                     SQYFSSDSISCPRCLKTESAKGDIHYHHQILQAVIVHPDLRQVLPLFPEPIQGSDCAE
                     RQDCEVNAGKRIIATIRTCPSSSPPTVSTPDSLSSMSLKATACPLS"
     gene            918..1541
                     /locus_tag="P5573_04265"
     CDS             918..1541
                     /locus_tag="P5573_04265"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27307.1"
                     /translation="MDVLKSNGMSFILTAKHSDHKILFEWVEELRAMGATDEWEVVDK
                     KGKRHRYQWIHDVPLNGNSNAPQVNFFQYWIISGEKTTYHNSWVTDIPVDHNNVMMLV
                     KAGRARWKIENETFNTLKNQGYHIEHNYGHGKQYISMNFFVLNLLAFYVHQILELCDL
                     SYQQCRRKFTSRKEFWNQLRCTFRIIPFRDYYHLLDYIFAPPTVRAP"
     gene            1889..2860
                     /locus_tag="P5573_04270"
     CDS             1889..2860
                     /locus_tag="P5573_04270"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRT27308.1"
                     /translation="MNTVLAYLLLVFEALILLIIYLPAPACAAGGVNVGELKSDPDRV
                     PYKGAADDASEPEVARKICVVGIAKEGYFPFILIKPDGTTNGFDRRILSLIAYELGFD
                     VKYRPVTWIDLMPNLKTHNIDVIASGMSITEERMAEVNFTNPYLWVSNVLVGREDKNL
                     NVGTALTHNRIGVEKGTAAAVWMENNLIKKGKKISLVYYESEAVLIDDVISGKIAVAA
                     LDDTAARHAKRTKPLKILGPYGMPDTPLGYAVRKDDTASLEALNSGLNRIMASLYWRA
                     NRLKYFGWSQSTKPKTARETLSRFTMTLPKNWTRQQDACYAPCHSSF"
     gene            3044..3598
                     /locus_tag="P5573_04275"
     CDS             3044..3598
                     /locus_tag="P5573_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27309.1"
                     /translation="MRRALIFCMSLLAITFFFIAAVGVYAETNEDLCKQWCSQHSDIC
                     KKCSPQVGCGIGYQSIVTFKINPAVWHACKGDETNKEACEAWCKEHKPQCEKCDDKVE
                     CGVGYKTIMRFTGGGGKGYFACEKTALKAASEDNKVLCEEYCNANKPQCAKCSKVAGC
                     GLGYEVIQSFGGHGVPWFACKKRK"
     gene            complement(3832..4605)
                     /locus_tag="P5573_04280"
     CDS             complement(3832..4605)
                     /locus_tag="P5573_04280"
                     /inference="COORDINATES: protein motif:HMM:NF014635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HRT27310.1"
                     /translation="MKTWQDLGKELSLYVKPETMPVAIKFLQDKKDIPVGTRTPLKNL
                     KVRMAHCQAQAIARKYGWTIALTKEDLGCAISGHTYGWEQADKDGAVRFLTKMNYASG
                     SETANTIFQSLRTLKPGRCEAVVYSPLERTKVEPDVILMYLNPAQLMRCLHGSTYHSG
                     QTIICSFSGRAASCTEGVIGAFLDQSPKVVVPGNGDRVWATVQDHEMAYVLPASHLKY
                     LVAGLAKTHEKGIRYPIPTFLRYQPEVGLTLPLTDIFKS"
BASE COUNT         1203 a         1239 c         1178 g         1168 t
ORIGIN      
        1 tagccgaagc ggtattgctc tttaacataa tcgcagggaa gagtcggttg gcttccagca
       61 attctcgctg aagatttttc gtgcacgggg cggggtacat atgtgtatcg gaatgatatc
      121 tttcgtggag tatgtaatcc ccgaaaatgt ataactactg aatatcacat cataaataat
      181 tctagacttt tcctttccga tagagtatat atctgtgtgg gttacgactt ggtagtagta
      241 ggaagagtct ggtgcacctc aagaagcatc tcaactttac tgccttaaga gctgccatgg
      301 cccagtgctt ccggcagata gaagaccgtc ggcaggtggg gaaagtgggc tattgcctgc
      361 atgacgtctt gatgagcggt tttgccatga tgtattttca ggacccttcg attcttagtt
      421 ttcagcgaag gttgcaggaa tgtactcagt tcaacaacct gaccaccgtc tttgacatcg
      481 gctctatccc caaagagacc cagatgcgcg atgttatcgc gtctcatccc cacccgggag
      541 ttgagcggca tattctcggt ctttcttcag cgattgcagc gtggtcagca gctttcccgc
      601 tatcggcttc ttggcggaaa gtatctcgtg gccatcgacg gttcccaata tttctcttcc
      661 gacagtatca gttgtccccg gtgtttgaag acagaatcgg ccaaggggga tatccactat
      721 catcaccaga ttctgcaggc ggttatcgtc catccggacc tacgccaggt gctgccgcta
      781 ttcccggaac ctatccaggg gagcgattgc gctgagaggc aggactgcga ggtcaatgcg
      841 ggaaaacgga tcatcgccac gatccgcacc tgcccatcat cgtcaccgcc gacggtctct
      901 actccagaca gcctttcatc gatgtcctta aaagcaacgg catgtccttt atcctgacgg
      961 ccaagcactc ggaccacaag attctcttcg agtgggtcga ggaactgagg gccatgggcg
     1021 caacagatga gtgggaagtg gtagataaaa agggaaagcg gcataggtat cagtggattc
     1081 atgatgtccc cctgaacggc aacagtaacg ccccgcaggt caatttcttc cagtattgga
     1141 tcataagcgg ggaaaagacc acctaccata acagttgggt tacggacatc cctgtcgatc
     1201 acaacaatgt catgatgctg gtgaaggccg gccgcgcccg ctggaagatc gaaaacgaga
     1261 ccttcaacac cttaaagaac caaggctacc acatcgagca taactacggg cacggcaagc
     1321 agtacatctc catgaacttt ttcgttctca atctcctggc cttctacgtc caccagatac
     1381 ttgagctgtg cgacctctcc tatcaacagt gccgccgtaa gttcacctcc cgaaaagaat
     1441 tctggaatca gttgcgctgc accttccgga tcatcccctt ccgagattat taccacctgc
     1501 tcgactacat cttcgctccc cctacggtca gggcacccta atgccacacg accgccttgc
     1561 cttcccctcg cttctgtctc ctgcgatgcc ccatcgccga ggctctcctt tgccttgctt
     1621 acaccaaaac cccaccctgc ccatactaaa ccactctcta tatgcctcat gccaccttcc
     1681 cacaccccca ctctacctct ctgcacccca ctgaaaacga ctctttactc tacgtacctc
     1741 ccacatctac ataaatacat ctcttcaacg agaattgcgc gggaggcctt ttatcttgac
     1801 atgccatgcc cgagtccgct aatttcaaat actcagtcat cagctatttt caccgtcctt
     1861 atcaaggtga tttccaatgc atcgctccat gaatacagta ctggcgtatc tcctcctcgt
     1921 ctttgaggcg ctcatcttat taataatata tctcccggcc cccgcctgtg cagccggagg
     1981 agtgaatgtc ggcgagttga aaagcgaccc ggatcgtgtc ccctataagg gggcagcgga
     2041 cgatgcaagc gagccggaag tggcccgaaa aatttgcgta gtcggtatag cgaaggaagg
     2101 atatttcccc ttcatactca taaaaccgga cgggacgacg aacggctttg atagacggat
     2161 attatccttg attgcgtacg aattggggtt tgatgtgaaa tatcggcctg taacgtggat
     2221 cgacctcatg ccgaacctga aaacacataa tatcgacgtg attgcatcag gaatgagtat
     2281 aacggaagag cggatggcgg aggtaaattt cactaacccg tacttgtggg tttccaacgt
     2341 cctcgtggga cgggaagaca agaatttaaa tgttggcacg gcgcttaccc acaacagaat
     2401 cggcgtcgaa aaaggtactg cggcggcggt ctggatggaa aacaatctta tcaagaaagg
     2461 gaaaaaaatc tcgctggttt attatgaatc ggaagccgtc ctaatcgacg acgtaatcag
     2521 cggcaagatc gccgttgctg ctcttgatga taccgccgca aggcacgcga aaagaacaaa
     2581 acctctcaag atattagggc cctatggtat gcctgacacc cctttagggt atgctgtccg
     2641 caaagatgac acggcatctc tggaagcctt gaattccggc ctgaatcgca tcatggcttc
     2701 actgtactgg agggcgaata gactcaagta tttcggctgg tcacagagca caaaacccaa
     2761 aacggcgagg gaaactctca gtcgctttac tatgactctg cccaaaaact ggacaaggca
     2821 acaagacgca tgttacgcgc catgccattc ctccttctga catcggtagg ctgacatgct
     2881 ttttccattt cccggtacga agtggtcctg ccaaccacta tattttgtta gacggtgggt
     2941 gcgttccagt agagatatga tgaagcgatg aagcccgtag gtttaataaa caatgtgacc
     3001 gagtcggttt tcgagagccc gtctttagga aaggagttgg aaaatgagaa gagcgcttat
     3061 tttttgcatg agccttttgg cgattacctt tttcttcatt gctgcggttg gagtgtatgc
     3121 ggaaaccaat gaggacctgt gtaaacagtg gtgcagtcag cattcggata tatgtaaaaa
     3181 atgttctccc caggtcggtt gcgggatcgg gtatcaatct atagtgacgt tcaaaatcaa
     3241 tccggctgtt tggcatgcct gcaagggcga cgaaacgaac aaagaagcct gtgaagcctg
     3301 gtgcaaagaa cataaacctc aatgcgagaa atgcgatgat aaggtcgaat gcggggtagg
     3361 atataaaacg attatgaggt tcacaggagg cggcggcaag ggttacttcg cctgcgagaa
     3421 aaccgcactc aaggccgcgt cagaagacaa caaagtacta tgcgaagagt attgcaatgc
     3481 caataagcct caatgtgcca agtgcagcaa ggttgccggc tgcggacttg gatatgaagt
     3541 gatccaatct tttggcggtc acggtgtccc ctggttcgca tgcaagaaga gaaaatagtc
     3601 tgctgacggg cttcatcttc tctaaaaaag ccttgcggaa tgtaaggaag cgcaggcagc
     3661 gtcttataac gaacaaaccc ctttttcggg atgaaacttt tatccatgat cgggcggtaa
     3721 agaatcgtaa tcacgagcga agcggggctg tttccctcgt tttgagaacc ccacgttcgg
     3781 aagcggcagc tgaacaaggt ggattttgtc cagcaaatat cggcacatag gttatgactt
     3841 aaagatatct gtcaggggaa gcgtgagccc tacttctggt tgatagcgca aaaaagtagg
     3901 tatgggataa cgaattcctt tctcatgggt cttggcaagg cccgcaacaa gatatttcag
     3961 gtgtgaagca ggtaacacat aggccatttc atggtcctgg accgtcgccc aaactcggtc
     4021 tccgttgccg ggtacaacga ctttgggaga ttgatccaaa aaagccccta taactccttc
     4081 cgtgcacgaa gcggcccgcc cggaaaacga acagataatc gtttgacccg agtggtatgt
     4141 gctgccgtgc aaacaacgca tgagttgtgc cgggttcaaa tacatcagaa tgacatccgg
     4201 ttcgaccttg gtccgctcca atggtgaata gacgacggct tcgcatcggc ccggtttcag
     4261 ggtcctgagg gattgaaaga tcgtatttgc agtctcactt cctgaggcat agttcatctt
     4321 tgttaggaat ctgaccgcac cgtctttatc ggcctgttcc caaccgtagg tatgcccgga
     4381 aatcgcgcat cccaaatcct ctttcgtcag cgcaattgtc caaccgtatt tccgggcaat
     4441 cgcctgggcc tggcagtggg ccatcctgac tttgagattc ttcaacggag tcctcgtacc
     4501 gaccggtatg tcttttttat cttgtaagaa ttttatcgcc acaggcatgg tttcaggttt
     4561 gacataaagg ctcagttcct tgccaagatc ttgccaagtc ttcattggaa atacctccta
     4621 attgcttccc gtatgcacga ggccggtctc ctctgatcgt ttcaggactt ctcgggcttc
     4681 ccccagggtg atgatttttg caataccacg ctccgccaga aaccgggcgg cgccgtcgaa
     4741 gcagaggcaa acatccaacg gcttttcgca gtttggactg cacccccc
//
LOCUS       DAOTMX010000111         4784 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_14648_length_4784_cov_3.772891, whole genome shotgun sequence.
ACCESSION   DAOTMX010000111 DAOTMX010000000
VERSION     DAOTMX010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4784
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14648_length_4784_cov_3.772891"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..102
                     /locus_tag="P5573_04285"
     CDS             <1..102
                     /locus_tag="P5573_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uL22 family ribosomal protein"
                     /protein_id="HRT27311.1"
                     /translation="QGTSWRRWRARAMGRAAQIKKRTSHITVVLAEQ"
     gene            122..769
                     /gene="rpsC"
                     /locus_tag="P5573_04290"
                     /pseudo
     CDS             122..769
                     /gene="rpsC"
                     /locus_tag="P5573_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416220.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
     gene            812..1234
                     /gene="rplP"
                     /locus_tag="P5573_04295"
     CDS             812..1234
                     /gene="rplP"
                     /locus_tag="P5573_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HRT27312.1"
                     /translation="MLTPKRVKYRKMQKGRMGGKAIKGCHVEFGAFGLQATEPGRITA
                     QQIEAARIAMTRHIKRGGRIWIRIFPHKSFTKKPAETRMGKGKGGPEGWIASVKPGTV
                     MYEMEGVTREVAREAFRLAAHKLPVGTKFVSRDFLDED"
     gene            1224..1418
                     /gene="rpmC"
                     /locus_tag="P5573_04300"
     CDS             1224..1418
                     /gene="rpmC"
                     /locus_tag="P5573_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HRT27313.1"
                     /translation="MKIKEMRDLSVDELKQKERELVDELFRLRVRHATGQLDSSAMMK
                     NVKRDIARIKTVLKEREIRG"
     gene            1421..1681
                     /gene="rpsQ"
                     /locus_tag="P5573_04305"
     CDS             1421..1681
                     /gene="rpsQ"
                     /locus_tag="P5573_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HRT27314.1"
                     /translation="MVEPRGNKRTVQGWVVSNKMDKTVVVRSERRVKHRTFQKYVRKI
                     KKYKAHDEGNACSIGDKVLIEESRPLSRDKRWRVREILEKAK"
     gene            1729..2097
                     /gene="rplN"
                     /locus_tag="P5573_04310"
     CDS             1729..2097
                     /gene="rplN"
                     /locus_tag="P5573_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HRT27315.1"
                     /translation="MIQMRTILNVADNSGAKRVSCIGVLGGSRKRYATVGDVITVSVR
                     EAIPNSKVKKGDVMRAVVVRTVKEVRRLDGSFLKFDDNSVVLINNQMEPVGTRIFGPV
                     ARELRAKQFMKIISLAPEVI"
     gene            2119..2439
                     /gene="rplX"
                     /locus_tag="P5573_04315"
     CDS             2119..2439
                     /gene="rplX"
                     /locus_tag="P5573_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HRT27316.1"
                     /translation="MQKIKLKKGDVVKVLAGKEQGKSGKILKFSKDRTRAFVEKLAMV
                     KRHKRPDAKGKGGIMEKEGAIDVSNLALICRKCERVVRIGYRVLEDDRKVRVCKKCNE
                     MLDE"
     gene            2455..2994
                     /gene="rplE"
                     /locus_tag="P5573_04320"
     CDS             2455..2994
                     /gene="rplE"
                     /locus_tag="P5573_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HRT27317.1"
                     /translation="MARLKEYYSKTVVPEMMKKFKYKNVMEVPRLEKIVLNMGVGEAI
                     QNIKLLDGAVQELAMISGQKPVITKAKKSIATFKLRKGMPIGCTVTLRKERMYEFYDR
                     LVNVALPRIRDFRGIPPGSFDGRGNFAMGIREQFIFPEIEYDKIEKVKGLNIVVVTTA
                     KTDDEARHLLSLMGMPFRS"
     gene            3025..3210
                     /locus_tag="P5573_04325"
     CDS             3025..3210
                     /locus_tag="P5573_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HRT27318.1"
                     /translation="MAKKSLIAKAKRTKKFKVREYNRCPLCGRPRAYYRKFDMCRLCL
                     RKYASQGQIPGMIKSSW"
     gene            3231..3632
                     /gene="rpsH"
                     /locus_tag="P5573_04330"
     CDS             3231..3632
                     /gene="rpsH"
                     /locus_tag="P5573_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HRT27319.1"
                     /translation="MGMTDPIADMLTRIRNANRKKFKSTDTGLSRMNVEIAKVLKKAG
                     YIQGFDIKKDPHKRDILRVYLKYSESKENILRGIERVSKPGRRIYVPSDSIPKVLNGY
                     GVAILSTSKGILTDREARELNVGGEILCNVW"
     gene            3673..4215
                     /gene="rplF"
                     /locus_tag="P5573_04335"
     CDS             3673..4215
                     /gene="rplF"
                     /locus_tag="P5573_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HRT27320.1"
                     /translation="MSRIGKKPIVLPANVKVTRKNAEIVVEGPKGKLSMKLPGEIDVI
                     QEAGSIILKKAVDNRRGRSLFGLTRTLVHNMVTGVSDGFQKNLEISGVGYRAELTGSD
                     LLKLHIGYSTPLEYRLPEGVSVKIEKQTSVTLTGIDKELVGRVAAEIRSLRKPEPYKG
                     KGIKYAGEKVRRKVGKSAGA"
     gene            4297..4632
                     /gene="rplR"
                     /locus_tag="P5573_04340"
     CDS             4297..4632
                     /gene="rplR"
                     /locus_tag="P5573_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017794725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HRT27321.1"
                     /translation="MKRKKRVRGKISGTADHPRISVFRSTTHIYAQAIDDAAGQTVAS
                     ASTLSKELKGKLGGMKKTQAAAEVGKLLAKQLKDRNIQKAVFDRGAFLYHGRVKALAE
                     AAKEAGLKI"
     gene            4667..>4784
                     /gene="rpsE"
                     /locus_tag="P5573_04345"
     CDS             4667..>4784
                     /gene="rpsE"
                     /locus_tag="P5573_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416232.1"
                     /note="located at the back of the 30S subunit body where
                     it stabilizes the conformation of the head with respect to
                     the body; contacts S4 and S8; with S4 and S12 plays a role
                     in translational accuracy; mutations in this gene result
                     in spectinomycin resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HRT27322.1"
                     /translation="MERREFEGTELLDRVVSINRTAKVVKGGRRFRFSAIVVV"
BASE COUNT         1210 a         1215 c         1585 g          774 t
ORIGIN      
        1 caggggacgt cctggcggcg gtggcgggcg agggccatgg gacgggcggc ccagatcaag
       61 aagaggacga gccacatcac ggtggttctg gcagagcagt agcgataagg gaggtacgga
      121 cttgggtcag aaggtcaatc cgatcggttt gaggctgggc atcgtcaaga cgtggggttc
      181 gaagtggttc tcggagaaga acttccggga actccttcac caggacatcc ggatccggcg
      241 gtaaatcaag gagaaactct accatgccgg tgtgtcgaag gtggagatcg aacgagccgc
      301 tgacaaagcg aagatcaaca tccacgccgc acgcccgggc atcatcatcg ggaaaaaagg
      361 ggcggaaatc gagaagctga agaaggacat cgaggccatc acgagggggg aagccatcat
      421 caatatcctg gaagtgcgca agcccgagat cgacgcccag ctcgtggcgg aaaatgtggc
      481 cctgcagctc gagaggcgtg tcgccttccg gcgggccatg aaaaaaggcg ttcagtccgc
      541 tctgaagttc ggtgccaagg gcattcggat cacctgtgcg gggcggctgg gcggcgccga
      601 gatgtccagg acggagtggt atcgagaagg caaagtgccc cttcacacgc tgagggcgga
      661 cattgattac ggtctggccg aggcgcggac gacctacggc gtgatcggcg tcaaggtgct
      721 catctatcac ggcgacatcc tgcccgacaa gaaaagcacg acggcctgag gcgcggaaga
      781 gggttttcat tgaagaggag ttcgtgacgg catgttgaca ccaaagcggg taaagtaccg
      841 caagatgcag aagggacgca tggggggcaa ggccatcaag gggtgccacg tggagttcgg
      901 cgccttcggt ctccaggcca cggaacccgg gcggatcacg gcgcagcaga tcgaggcggc
      961 ccgaatcgcc atgacccggc atatcaagcg cggcgggcgg atctggatcc ggatttttcc
     1021 ccacaaatcc ttcacgaaga aaccggcgga gacccgcatg ggcaagggca aaggcggccc
     1081 ggaagggtgg atcgcctccg tgaagcccgg aacggtgatg tacgagatgg agggcgtgac
     1141 ccgggaggtg gctcgcgaag ccttccggtt ggccgcccac aaactgccgg tgggcaccaa
     1201 attcgtctcg agggactttt tagatgaaga ttaaggagat gagggatctc agcgttgatg
     1261 agctgaaaca gaaagagcgc gaactggtcg acgagctctt ccggctcagg gtacggcacg
     1321 cgacggggca gctggactct tcggccatga tgaaaaacgt caagagggac atcgcccgta
     1381 tcaagaccgt tctgaaggaa agggagattc gaggttaggt atggtggaac cgcggggcaa
     1441 caagaggacc gtccagggct gggtcgtgag caacaagatg gacaagaccg ttgtggtccg
     1501 ttccgagagg cgggtcaagc acaggacctt tcagaagtac gtccgcaaga tcaagaaata
     1561 caaggcccat gacgagggga acgcctgcag catcggcgac aaggtcctca tcgaggaatc
     1621 ccggcccctg agccgggaca agcggtggcg ggtccgggag attcttgaaa aggcgaagta
     1681 aggcccgcct gcgaccgggg aaccggcggt gggatcggag tgttggttat gatacagatg
     1741 cgaaccatat tgaacgtggc ggacaattcc ggagcgaaac gggtcagctg tatcggcgtg
     1801 ctcggcggtt cgcgcaagcg ttatgcgacg gtgggcgatg tcattacggt gtcggtcagg
     1861 gaagccatcc ccaattcgaa ggtcaagaag ggggatgtca tgcgggcggt ggtggtgagg
     1921 accgtcaagg aagtgaggcg actcgacggg tcgttcctca agttcgacga caattccgtg
     1981 gtgctgatca acaaccagat ggaacccgtc ggaacccgca tcttcggccc ggtggcccgg
     2041 gagctgcgcg cgaaacagtt catgaaaatc atctccctgg cccccgaggt tatttaggaa
     2101 gggacccgag gaattcccat gcagaagatc aaattgaaaa aaggggatgt cgtgaaggtt
     2161 ctggccggca aggagcaggg aaaatccggc aagatcctga aattttccaa ggacaggacg
     2221 cgagccttcg tcgagaaact cgccatggtg aagcgccaca aacggcccga tgccaagggg
     2281 aaaggcggca tcatggagaa ggaaggggcc atcgacgtgt ccaacctggc cctcatctgc
     2341 cgcaaatgcg agcgcgtggt ccgcatcggg taccgtgtgc tggaggacga ccggaaagtc
     2401 cgcgtctgta aaaagtgcaa tgagatgctc gatgagtagg ccgaggtaag acccatggca
     2461 agattgaaag aatactacag caaaaccgtc gtcccagaga tgatgaagaa gttcaaatac
     2521 aagaatgtca tggaggtccc gcggctggag aagattgtcc tgaacatggg ggtcggcgag
     2581 gccattcaga acatcaagct tctcgacggc gccgtccagg aactcgccat gatctcgggg
     2641 cagaagccgg tgatcacgaa ggcgaagaag tccattgcga cgttcaagct tcgaaaggga
     2701 atgccgatcg gctgcaccgt gacgctccgg aaggagcgga tgtacgaatt ctacgaccgg
     2761 ctggtgaacg tggcgctgcc gagaatccgg gacttcaggg gtattccccc cggctccttc
     2821 gacgggcggg gaaatttcgc catggggatc cgggaacagt tcattttccc cgagatcgag
     2881 tacgacaaga tcgaaaaggt caaaggcttg aacatcgtcg tcgtgacgac ggcaaaaacg
     2941 gacgatgagg cgaggcacct gctctccctc atgggcatgc ccttcaggag ctaacccgta
     3001 aacggtgcgg atggaggaac cgacttggcg aagaaatccc tgattgcgaa agcgaagagg
     3061 acgaaaaaat tcaaggtcag agaatacaac cgctgccccc tctgcggcag gcccagggcc
     3121 tattaccgga aattcgacat gtgcaggctc tgcctgagaa aatacgcgtc ccaggggcag
     3181 atccccggga tgatcaaatc cagctggtga gggttttcag gagaagcact atgggaatga
     3241 cggatccaat cgcagacatg ttgacgagaa tccggaacgc caatcggaag aaattcaaga
     3301 gcaccgacac ggggctttcg agaatgaacg tggaaattgc caaggtcctg aagaaggccg
     3361 ggtatattca ggggttcgac atcaaaaaag acccgcacaa gcgggacatc ctgagggttt
     3421 atctcaaata ctccgagtcg aaggaaaaca tccttcgcgg gatcgaacgg gtgagcaagc
     3481 ccggacgccg tatctatgtg ccgagcgaca gcatccccaa ggtcctcaac ggttacggtg
     3541 tcgccatcct gtccacctcc aagggcatcc tgacggaccg ggaggcccgg gaactgaacg
     3601 tgggcggcga gatcctctgc aacgtctggt aagtccggcg acgcagcatt tgtttgagtg
     3661 ggagagccat acatgtcaag aatcggcaaa aaacctattg tcctgccggc caatgtgaag
     3721 gtcacccgga agaacgcgga aatcgtcgtc gaggggccga aggggaaact ctccatgaag
     3781 ctccccgggg aaatcgacgt gatccaggag gcggggagca tcatcctcaa gaaggctgtg
     3841 gacaaccgcc gcggacgttc cctcttcggg ttgacacgaa cgttggtcca caacatggtc
     3901 acgggggtca gtgacgggtt tcagaaaaac ctggagatct ccggtgtggg gtaccgggcg
     3961 gaactgaccg gcagcgacct cctgaaattg catatcggat attccacgcc cctggagtac
     4021 cggctccccg agggggtgtc cgtcaagatc gagaagcaga ccagcgtgac cctgaccggg
     4081 atcgataagg aactcgtcgg ccgcgtagcc gccgagatcc gcagcctcag gaaaccggaa
     4141 ccctacaaag ggaaagggat caaatatgcc ggcgaaaagg tccgcaggaa ggtcggcaag
     4201 tccgccggtg cctaaaaggt gaccggagcc ggcacgggat gacaaaggaa ggcgaaagcg
     4261 gtgatcggga aaacgaagaa gatcaagaag atcaggatga agagaaagaa gagggtgcgg
     4321 ggcaagatct ccggtacggc ggatcacccc cggatttcgg tcttcagaag cacgacgcat
     4381 atctatgccc aggcgatcga cgacgccgcc ggacagacgg tggcctcggc ctcgacactc
     4441 agcaaggagc tcaagggcaa actcggcggc atgaagaaaa cccaggcggc cgccgaagtg
     4501 gggaagcttc tggcgaagca gctgaaggac aggaacattc aaaaggccgt tttcgaccgt
     4561 ggcgcgttcc tctatcacgg cagggtcaag gcgctggccg aagcggcgaa agaagcaggc
     4621 ctgaagattt gagcccgggc tttcatttca ttgaggggtg ggatcattgg agagacgaga
     4681 attcgaagga acggaacttc tggacagggt ggtttccatc aaccggacgg ccaaggtcgt
     4741 aaaagggggc cggcggttcc ggttcagcgc catcgtggtc gtcg
//
LOCUS       DAOTMX010000112         4778 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_14670_length_4778_cov_3.635825, whole genome shotgun sequence.
ACCESSION   DAOTMX010000112 DAOTMX010000000
VERSION     DAOTMX010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4778
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14670_length_4778_cov_3.635825"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="P5573_04350"
     CDS             <1..478
                     /locus_tag="P5573_04350"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRT27323.1"
                     /translation="GGLALLFSGFLDTLDGSVARLTGRSNPAGALLDSTLDRYAELAV
                     FSGLLFYYRHGWMFAFVVLALVGSVMASYVKARAQSLGKVRTVGLMQRPERLALLIVG
                     SLANPLVELFLPEYPDVALSTAIVVLAVLSNGTALRRLVEGRKDLSPRISSLTKDS"
     gene            589..1071
                     /gene="greA"
                     /locus_tag="P5573_04355"
     CDS             589..1071
                     /gene="greA"
                     /locus_tag="P5573_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HRT27324.1"
                     /translation="MEKVPITRSGFEKLKKDLEYLKKVAVPENIRAIEEARGHGDISE
                     NAEYAAAKERQSFIQGKIQEIENNLAISKVIDIENLSEEKVVFGSTVTIEDTETGEIT
                     AYQLVGPFESDLQQNKISVTSPIGKALIGKVAGDEVRVKTPGGLRVLQVMDIRIGADG
                     "
     gene            complement(1094..1669)
                     /locus_tag="P5573_04360"
     CDS             complement(1094..1669)
                     /locus_tag="P5573_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27325.1"
                     /translation="MTNSPYVEVVLEGSIDLIKGFVIGFLEGRGIRGKAVFEEEGSAG
                     EGGSLAQLLRLVSLKEDRIHVIVQEGVHELLAEALQKCKTDLPIKVVSVRRIREASFD
                     FRFHTYSKSAGRELKSLFQNLPEGLCLKGYQPREVLNPDAKGIEAYAPEHDYEIQAEG
                     TVRGEPAKVTELYNRLKHFELVELGEIRMED"
     gene            complement(1730..3184)
                     /locus_tag="P5573_04365"
     CDS             complement(1730..3184)
                     /locus_tag="P5573_04365"
                     /inference="COORDINATES: protein motif:HMM:NF028290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HRT27326.1"
                     /translation="MAQMKRSNLFPFLVGAFLLLGAVEVCAAESSGGGIGAVLPMWAC
                     VPFVGILLSIALGPLLVPHFWHDHYGKVSAFWALAFALPFLIFFHGEALYHILHIYLI
                     DYIPFIILLWALFTISGGIVVGGTIRGTPPANTTMLLVGTLLASWVGTTGAAMVMIRP
                     VLRANRDRERKAHIVCFFIFLVANIGGALTPLGDPPLFLGFLHNVPFFWVTKAILPHM
                     LFTASTVLITFFVVDSIYYRKERPLGGERGNKKSIRINGAYNFFFLGGVVAMVLMSGY
                     WKAGYFSVWGVEVEIQNLLRDAGILAMGLLSLRFTPEPLRRANEFGWAPIVEVAKLFA
                     GIFMTIIPAIAILKAGSDGAMAPLLTLVNEPAHYFWATGVLSSFLDNAPTYLTFFNMA
                     LGKLGMTEAMVPAALDAAAATAHPVFAGYLKAISAGAVFMGANTYIGNAPNFMVKSIA
                     EEAGVAMPSFFGFMLKYSIPVLIPVFILITYIFF"
     gene            complement(3257..3667)
                     /locus_tag="P5573_04370"
     CDS             complement(3257..3667)
                     /locus_tag="P5573_04370"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HRT27327.1"
                     /translation="MADELMLEIVTPEKVAFSGKVEEVTIPGTEGEFGVLVGHASLLS
                     AVKFGELNYTKDNKKTYYAVNTGYAEVTSHKVTVLVETAEKADVIDVERARQAKEIAA
                     EKLAKMSKDDPDYEKIKNALDRAEIRLKIAERAS"
     gene            complement(3683..>4778)
                     /gene="atpD"
                     /locus_tag="P5573_04375"
     CDS             complement(3683..>4778)
                     /gene="atpD"
                     /locus_tag="P5573_04375"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HRT27328.1"
                     /translation="GPIDAKVKMPIHREAPSFTEQDTSVHVLETGVKVIDLLVPFPRG
                     GKMGMFGGAGVGKTVVMMEMIHNIAMHHGGISVFGGVGERTREGNDLYLEMKQSGVIS
                     KAALVYGQMTEPPGARARVALTALTAAEYFRDVEGQDVLLFIDNIFRFTQAGAEVSAL
                     LGRMPSAVGYQPTLATDLGELQERITSTTKGSITAVQCVYVPADDLTDPAPATTFSHL
                     DGTVVLSRPIAELGIYPAVDPLDSTSRILDPNVIGEDHYMVARNVQMILQKYKDLQDI
                     IAILGMDELSEEDKLTVSRARKIQRFLSQPFFVAAQFTGTDGKFVSVKDTVQGFKEIL
                     EGKHDDLPEQAFYMVGGIEEAVEKAKRLSE"
BASE COUNT         1116 a         1361 c         1254 g         1047 t
ORIGIN      
        1 tggagggctg gccctgctct tctccgggtt cctggacacc ctcgacgggt ccgtggccag
       61 attgaccggt cgctccaacc ccgcaggggc gttgctcgat tctacgctgg accgatatgc
      121 cgagcttgca gtcttctcgg ggctgctttt ttattaccgg cacgggtgga tgttcgcctt
      181 cgtcgtcctc gccctcgtgg gctcggttat ggcaagttac gtcaaggcac gggcacaatc
      241 tctcggcaag gtgcgtacag tcggcctcat gcaaaggccc gagaggctcg ccctcctcat
      301 cgtgggctcc ctcgcaaatc ctcttgtcga attgtttctg cctgaatacc cggacgtagc
      361 tctttcgacg gcaatcgtcg ttctggcggt cctttcgaac gggacggccc tccgacggct
      421 tgtcgagggg aggaaggacc tttcgccacg tatttccagt ttgacaaagg actcgtgatt
      481 aaagtatcct tacaataact caaaaatgaa ggtcgtcaaa aacacccctc ttcagcaaga
      541 ggggatgtgt ttttttatag caaaatcatc ggcctaacgg agaacaagat ggaaaaggtg
      601 cctatcaccc gctcgggatt cgaaaaactg aaaaaagact tggaatacct aaaaaaagtc
      661 gccgtaccgg aaaacatacg ggcgatcgaa gaggcccgcg gtcacggaga tatctccgag
      721 aacgcggaat atgcagcggc gaaagagcgc cagtccttca tccagggaaa gattcaagag
      781 atagaaaaca acctggccat ctccaaggtc atagacatcg aaaacttgag tgaagaaaag
      841 gtcgtcttcg gttcaaccgt cacgatagaa gatactgaaa cgggcgaaat aaccgcctac
      901 cagctcgtgg gaccgttcga atccgatctc caacagaaca agatctccgt cacatctccc
      961 atagggaaag ccctgatcgg aaaagtcgcc ggcgacgagg tccgcgtaaa aactcccggc
     1021 ggcctaaggg tcctgcaggt catggacatc cgcatcggcg cggatggata atcaacctac
     1081 cgaacacagg gagtcaatct tccatgcgaa tttccccgag ttccacgagt tcaaagtgct
     1141 taaggcggtt gtaaagttcg gtaactttgg ccggctcgcc ccttaccgtc ccctcggcct
     1201 ggatctcgta gtcgtgctcc ggggcgtaag cctcgattcc tttggcgtcg gggttcaaaa
     1261 cttcgcgggg ctgataacct ttgaggcata ggccctccgg cagattctgg aaaaggcttt
     1321 tcaactccct gcccgcagac tttgaatagg tatggaagcg gaagtcgaag ctcgcctccc
     1381 tgattctcct taccgagaca accttgatcg gcaaatccgt cttacacttc tgtagcgcct
     1441 ctgcgagcag ttcgtgaacc ccttcctgga cgattacatg gattctgtcc tctttcaaac
     1501 tgaccaaacg cagcagttgt gcgagagatc ccccttcacc tgcgctgccc tcttcctcga
     1561 aaacggcctt cccccgaata ccgcgccctt ccagaaaacc gatcacaaaa ccttttatga
     1621 gatcgatcga accttcgagt acaacctcaa catacggcga gttcgtcatg acccctcctt
     1681 ttttcgaagc ctgcacctcg ttatccccgc tttctatcgc tcgcactgct cagaaaaaaa
     1741 tataagtaat caggataaat acagggatca gcacaggtat agagtatttc agcatgaagc
     1801 cgaagaagct cggcatggcc acaccggcct cctccgcgat ggacttcacc atgaagttcg
     1861 gggcgttgcc gatatatgta ttggccccca taaaaacggc tcctgcggag attgccttca
     1921 ggtaaccggc aaagactggg tgcgcagttg ctgcggccgc gtcaagggcg gcgggcacca
     1981 tcgcctccgt catccccagc ttgcccaggg ccatgttgaa aaaagtcagg taagtagggg
     2041 cattgtcgag aaaagatgag agcacccctg tagcccagaa gtaatgagcc ggttcgttca
     2101 ctaaggtgag aaggggcgcc attgcaccat cgcttcccgc tttgaggatg gcgatggccg
     2161 gtattatcgt cataaaaatg cccgcaaata gtttcgccac ttcgacaatc ggcgcccagc
     2221 cgaactcgtt cgcccgccgg agcggctcgg gcgtaaaacg aagggaaaga agacccatgg
     2281 cgaggatacc ggcgtcccgt aagaggttct gaatctcgac ctcaactccc catacggaaa
     2341 aatatccagc cttccagtag ccgctcatca gaaccatcgc cactacgccg cccaggaaga
     2401 aaaaattgta cgcaccattt attcttattg atttcttgtt ccctctttct cccccaaggg
     2461 ggcgctcttt cctgtagtag atgctgtcga caacgaaaaa ggtaatgagc actgtggagg
     2521 cggtaaagag catatgagga aggattgcct tagtcaccca gaagaacggg acgttgtgaa
     2581 gaaaacccag gaaaagcggc ggatcaccga ggggcgtcaa ggcgccgccg atgttggcca
     2641 caaggaatat gaaaaaacag acgatatggg ccttgcgttc ccggtcccgg ttagccctga
     2701 gaaccggccg tatcatcacc atcgccgcgc ccgtcgtccc cacccaggaa gcaaggaggg
     2761 tgccgacaag aagcatagtt gtgttggcag gcggcgtgcc tcttatggtt ccgcccacca
     2821 cgatccctcc cgagatagta aagagggccc agagtaggat aatgaacggt atgtaatcga
     2881 tcagatagat atggaggatg tggtagaggg cttcaccatg aaagaagata aggaacggca
     2941 gggcaaaggc aagtgcccag aaggccgaga ccttcccgta atggtcatgc cagaaatgtg
     3001 gcaccaggag aggaccgagg gcgatggaaa gcaaaatgcc gacaaaaggt acacaggccc
     3061 acatcggcag caccgcgcca atcccgccgc ccgacgactc ggcagcgcag acttcaactg
     3121 caccgaggag aagaaacgcg ccgacgagaa acggaaagag attgctcctt ttcatttgtg
     3181 ccattccctc aaacgaaaag ccagtaagga tgaccttccc tggctttttg tttgaaattt
     3241 tctccctgtc gtcgcgctat gaagctcttt cggcgatctt caaccttatc tccgcccggt
     3301 cgagggcatt ctttattttt tcgtagtcag ggtcgtcctt cgacatcttt gctaactttt
     3361 ccgccgcgat ctcttttgcc tgtcgcgccc tttctacatc gatcacatcg gccttctccg
     3421 cagtctccac aagtaccgtc actttatgcg acgtcacttc cgcgtacccg gtgttcacag
     3481 cgtaataggt ctttttgtta tctttagtgt agttcaattc tccgaatttt acggcgctca
     3541 gcagcgaggc atggcctaca agaacaccga actcaccctc cgtcccggga atggtgactt
     3601 cttcgacctt accgctgaag gccacttttt ccggcgttac gatttccagc atcaactcat
     3661 ctgccatcta aaaatcctcc ggttactcgg acagtctctt cgctttttcc acagcctcct
     3721 cgattccacc gaccatgtaa aaggcctgct ccggaagatc gtcatgtttg ccttccagta
     3781 tctccttgaa cccctggact gtgtccttca cggagacgaa cttgccatcg gtaccggtaa
     3841 actgcgcggc gacaaagaac ggctgggaga ggaacctctg gatttttctg gctctgctga
     3901 ctgttagttt atcctcttcc gaaagttcgt ccattccgag aatggcgatg atgtcctgaa
     3961 ggtccttgta tttctgaagg atcatctgga cgttccgcgc caccatgtag tggtcctcgc
     4021 cgataacgtt ggggtcgagg attcgcgatg tagagtcgag ggggtccaca gcggggtaga
     4081 tgcccaactc ggcgatcggc cgcgagagaa cgaccgttcc gtcgaggtgc gagaaggtcg
     4141 tcgccggtgc ggggtccgta aggtcgtcgg cagggacgta gacgcactga acggctgtga
     4201 tggaaccttt cgtcgtcgat gtgatccgct cctgaagttc tccgaggtcc gtagccaagg
     4261 tcggctggta accgaccgct gaaggcatac ggccaagaag agccgagact tcagctccag
     4321 cttgggtgaa acgaaagata ttgtcgatga agaggagcac atcctgtccc tccacatcgc
     4381 ggaaatactc cgccgccgtg agggccgtaa gtgccacccg cgcgcgggct cccgggggtt
     4441 ccgtcatctg accgtagacg agggccgctt tcgagatgac tcccgactgt ttcatctcca
     4501 ggtaaaggtc attgccctct ctcgtccttt cacccacacc gccgaaaacg gagatacctc
     4561 cgtgatgcat ggcgatgtta tggatcatct ccatcatgac gacggtcttg ccgaccccgg
     4621 cgccgccgaa catccccatc ttgccgcctc tggggaaggg aacgaggagg tcgatgacct
     4681 tgactccagt ttcgagaacg tgcaccgaag tatcctgctc cgtaaagctg ggggcctctc
     4741 tgtggatcgg catcttgacc ttggcatcaa tagggccg
//
LOCUS       DAOTMX010000113         4762 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_14735_length_4762_cov_3.880816, whole genome shotgun sequence.
ACCESSION   DAOTMX010000113 DAOTMX010000000
VERSION     DAOTMX010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4762
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14735_length_4762_cov_3.880816"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="P5573_04380"
     CDS             <1..729
                     /locus_tag="P5573_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27329.1"
                     /translation="EDGRRLIRAALVRGADEVTLYCHPTDKEIKEEGIKNRFERRFEE
                     ALSKKHGTKRYDKVLEKVGRLKERFRRVAHRYEVSVDKDDGKDLATALRWELKEKAGQ
                     CGLYCLRSNRLDFKEQEMFDIFAMLTDIEDTFRSMKSELGLRPVYHQREDRCDGHLFI
                     TVLAYHVAHTIRTQLRKQGIADSWATIRKGLSSQVRITTTMKRDNGKVIHIRKSTRPE
                     PFHRLIYDALQVPHRPGKTVKTII"
     gene            complement(841..938)
                     /locus_tag="P5573_04385"
     tRNA            complement(841..938)
                     /locus_tag="P5573_04385"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(901..903),aa:Sec,seq:tca)
     gene            complement(1116..2357)
                     /locus_tag="P5573_04390"
     CDS             complement(1116..2357)
                     /locus_tag="P5573_04390"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HRT27330.1"
                     /translation="MITVKEALAQVLATVKPLPLEKVGLLDTLGRVLGEGIRSGRDIP
                     PKNNSAMDGYALRAVDTRGASIERPATLRVIEDVPAGYLWTKKIGAGEAVRIMTGAPI
                     PEGADAVVKVEDTENEGGDVRVFVSAVEGLNIRLAGGDVRVGELVIRKGSVVRPAEAG
                     MLAALGRSIVYVHQVPVVAIISTGDELADIDEPLSPAKITNSNGYALAAQVLECGAVP
                     LQVGIARDDRRELAEKFRMALRADAILSSGGVSVGDYDLVKEVMMALGTRMKFWRIAM
                     RPGRPLAFGTLKGKPLFGLPGNPVSSMVSFEQFVRPALRKMLGHRDLFRRAVRATLTE
                     SITKEKGLRYFVRGRVVYENGLYSVTTAGGQGSGILKSMVKANGLIVLPEDLTEAKKG
                     ETVSVQLIDRSLENAVSPEYL"
     gene            2587..3492
                     /gene="nadA"
                     /locus_tag="P5573_04395"
     CDS             2587..3492
                     /gene="nadA"
                     /locus_tag="P5573_04395"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HRT27331.1"
                     /translation="MNVAEKQDQIRGQLKEKKGVLLAHYYQRGEVQDVADFVGDSLAL
                     SIEAARTDAEVIVFAGVHFMAESASILSPEKTVLLPRLEAGCPLADTITPAELEKARA
                     EHPGCRVVTYVNSSAAVKALSDICCTSANAVKVVNSLAGEKILMVPDGNLARYTARFT
                     KKEVIPWEGYCPVHHFLTAEEVKEVMREHPGARLAVHPECRAEVIDMADFVGSTAGII
                     RYARETEAREVVIGTEVGVLHPLRKESPEKTFIMASEKMVCETMKSITLEDILSSLRE
                     MKHVVKVAEDTRVPAFRALDRMLKL"
     gene            3563..>4762
                     /locus_tag="P5573_04400"
     CDS             3563..>4762
                     /locus_tag="P5573_04400"
                     /inference="COORDINATES: protein motif:HMM:NF037626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27332.1"
                     /translation="MKRIVEDRTYLKDPVLRGFLEKVCAGDERVRVSGPDGSAKALLA
                     ALVRRFSGKTTLVVVPTLAEAERFCRDLSLFLRDGSILLYPPWDLLSQDFISSRGDVE
                     AGRVEVLSHLILGKSLLVVAPVDALMQKIRAGGAVRSYMQTVAIGDFLDREELLKKLD
                     EGGYRHVSLVEEKGEYSVRGHVVDVFPPSEPRPLRLIFIGDEVESIKTFDVDSQRTAE
                     ERVDFVLSPARELIVSEELRKVALRELKERADDINLPRLMRDRLQDMVGNGLPLNPRL
                     LPLFGGSGGEGCAGLFSFLPAGSVVILDDPYSLERAGNEYVEALDRAAAKAREEGRFY
                     LEHESYLFSGEELRRGLESFQEVVFDDLEVERREGGAWSVRFDVAKPSGFREEGVSFR
                     KSDRPLQP"
BASE COUNT         1033 a         1323 c         1415 g          991 t
ORIGIN      
        1 gaggatgggc gtagactgat cagggcggcc ctggtccgtg gtgccgatga agtcaccctc
       61 tattgccatc ccacagacaa agagatcaaa gaagagggga tcaagaatcg cttcgagcga
      121 cgcttcgagg aggccctgag caaaaaacac ggcaccaagc gctacgacaa ggtcttagag
      181 aaggtcggca gactgaagga gcggttccga cgggttgcgc accgctacga ggtaagtgtt
      241 gacaaagatg acggaaagga cctggcaacg gccttgcgat gggagctgaa ggagaaggcc
      301 ggtcagtgcg gcctgtactg cctgcggtcc aaccggttgg atttcaaaga gcaggaaatg
      361 ttcgacatct ttgccatgct cacggacatc gaggacacct tccggagcat gaagtcggag
      421 ttagggctgc gtcctgtgta ccaccaaagg gaagaccggt gcgatggaca tctcttcata
      481 accgtcctgg cgtaccacgt tgcacataca atccgcacac agctgcgaaa acaggggatc
      541 gccgacagtt gggctaccat ccgcaaaggc ctgtcctctc aggtacgcat aaccacaacg
      601 atgaaacggg acaacggcaa ggtgatccac atccgaaagt ccaccagacc tgaacccttc
      661 caccgcctga tctatgacgc cttacaggtg cctcatcgac caggcaaaac cgtaaagacg
      721 atcatctgag gtacgaaaat ctgtagtgcc taaacgatgc ctcctgaagc cataagcttt
      781 tgtttccata catgtatttt tttgatacca tgaactttgg ttaagaaaat caataaaaaa
      841 tggcggaagt gcatgggaat cgaacccacc tgagagagtt ttagtccctc acaccggatt
      901 tgaagtccgg gaggcccacc agtgaccttg gcacttccgc tgcgatttga atagcagaat
      961 ttatcgaacc gtgtcaatgg attatcgttt gtcgggaata agggcgtgaa gggggcttct
     1021 ttgaatttgt tcgaatccgc cgccacggca cgatgggagc caaaccaaag tctcgccatg
     1081 acggcataga cccggagtga aaacggcccc gcgtcttaaa ggtattctgg ggatactgcg
     1141 ttttcgaggg agcggtcgat gagctgaacg ctgacggtct cgcctttctt cgcctccgtc
     1201 agatcttccg gcagaacgat caacccgttc gccttgacca tagacttcag tattcctgac
     1261 ccctgcccgc ccgcggtggt cacggagtac agcccatttt cgtataccac ccgaccccgc
     1321 acgaaatagc ggaggccttt ttccttcgtt atactttctg tgagtgtagc gcgcaccgct
     1381 cgtctgaaaa ggtcgcggtg ccccagcatc ttgcggagtg ccggcctcac aaactgttcg
     1441 aaggagacca tcgaagaaac agggtttccc ggcagcccga aaaggggctt gcctttcagt
     1501 gtcccgaagg caaggggtct cccgggtctc atcgcgatcc tccagaattt catcctcgtt
     1561 cccaacgcca tcattacttc tttcacgagg tcgtaatctc ccacggatac gcctcccgac
     1621 gaaaggatcg catccgcccg gagggccatc cggaactttt cggccagttc tctcctgtcg
     1681 tctctggcga tgccgacctg caggggaacg gcgccgcatt ccaggacctg agccgctagg
     1741 gcatacccgt tgctgttcgt gatctttgcc ggggaaagcg gctcgtcgat gtcggcgagt
     1801 tcatcccccg tcgaaataat ggccacaacc ggtacttggt ggacgtaaac aatggaacgg
     1861 cccagggccg ccagcatgcc ggcttccgcc ggacggacga cgctgccttt cctgatgacc
     1921 agttcaccga ccctgacgtc ccccccggca aggcggatgt tcagcccttc cacggctgag
     1981 acaaagaccc tgacatcacc tccttcgttt tccgtgtctt ccaccttgac tacggcgtct
     2041 gctccttcag ggatcggtgc ccccgtcatg atgcggacgg cttcaccggc ccctatcttt
     2101 ttcgtccaca ggtaacccgc cgggacatcc tcgatgacgc ggagcgtggc cggcctctct
     2161 attgatgcgc cccgtgtatc gacggcacgg agggcgtagc cgtccatcgc ggaattgttt
     2221 ttcggcggga tgtcgcggcc ggagcggatg ccttctccca ggacccggcc gagagtatcg
     2281 agaaggccga ccttctccag cggaagcggt tttactgtcg caagtacctg cgcaagggct
     2341 tcttttacag taatcataag gaaacccgaa ggttgggatt tcgaaggaag aaagaagaac
     2401 tttcagtttg gaccggccgc tacatccttc aaaacgagaa ctatcctaaa attgatgact
     2461 tgtcaatgtc aggccatgtc cgttttcctt ttacgccttg aattattccc atcgatggag
     2521 taaagtttta cgttgatttc agctggttgg ggtctatcgg attaatcgtg attagagggc
     2581 ttgggaatga atgtcgcaga aaagcaggat cagatcaggg gtcagctcaa ggagaaaaag
     2641 ggggtgcttc tcgcccatta ctatcagcgt ggggaggtac aggatgtggc cgattttgtg
     2701 ggcgattccc tcgccctcag catagaagcg gccagaacgg atgccgaggt gattgttttt
     2761 gccggggtcc attttatggc ggagagcgcc tcgatcctgt ccccggaaaa gaccgtactt
     2821 cttccacggc tcgaggccgg ttgtcctctc gccgacacga tcacgcccgc cgaactcgag
     2881 aaagccaggg ccgaacatcc tggatgccgg gtggttacct acgtgaattc ctcggcggcc
     2941 gtcaaggccc tgagcgacat ctgctgtacc tcggcaaatg ccgtaaaagt ggtgaattcg
     3001 ctcgcgggcg aaaaaatcct catggttccg gacggcaacc ttgcgcggta cacggcgaga
     3061 ttcacaaaaa aagaagtcat tccctgggag gggtattgtc ccgttcatca ttttctcacg
     3121 gcggaagaag tgaaagaggt gatgagggaa catcccgggg cgaggctcgc cgtccacccc
     3181 gaatgcaggg ccgaggtgat cgacatggcc gacttcgtcg ggagcacggc aggcatcatc
     3241 cgttatgccc gtgagaccga ggccagggag gtcgtcatcg gaacggaggt cggggtcctg
     3301 caccccttga gaaaggaaag ccccgaaaag accttcatca tggcctcgga aaagatggtc
     3361 tgcgagacga tgaagtcgat cacgctggaa gacatcttga gttccctgag ggagatgaag
     3421 cacgtcgtca aggtggccga ggatacccgg gtgccggctt tcagggcctt ggaccggatg
     3481 ctaaaattat agcattgaag taataggtaa gggcgcaagg agtcgaggct gagaaccgaa
     3541 cggtgacggc cgacagttgc ggatgaaaag aatcgttgag gataggacgt acctgaaaga
     3601 cccggtgttg aggggtttcc tggagaaggt ctgcgccggc gacgaacggg tccgggtgag
     3661 cggcccggac ggctccgcta aggctctcct cgcagccctc gtccgccggt tctccggcaa
     3721 gacgaccctc gtcgtcgtgc cgacgttggc ggaagccgag cgcttctgcc gcgacctttc
     3781 cctcttccta cgtgacggaa gcatactcct ctatcctccc tgggacctcc tttcccagga
     3841 tttcatttcc tctcggggtg atgtggaggc gggccgggtc gaagtccttt ctcatctgat
     3901 cttgggaaag tccctgctcg tcgtcgcccc cgtagacgcc ctgatgcaga agataagggc
     3961 gggtggcgcg gtccgttctt acatgcagac cgtcgccata ggagacttcc tggatagaga
     4021 ggagctgctg aaaaagctcg acgagggcgg ataccggcat gtctctctcg tggaggagaa
     4081 gggtgagtac agcgtcaggg ggcatgtcgt cgacgtcttt ccgccgtcgg agcctcgacc
     4141 gctcaggctc atttttatcg gcgatgaagt ggagtcgatc aagaccttcg acgtcgattc
     4201 tcaacggacc gcggaagaaa gggtcgattt tgtcctttcc ccggcgaggg aactgatcgt
     4261 ctcggaggag ctgcggaagg ttgccctccg ggagttgaaa gagagggcag acgatatcaa
     4321 cctgccccgg ctgatgcgcg accgcctgca agatatggtc gggaacggcc ttcccctcaa
     4381 cccccggctt ctgccgcttt tcggcggttc gggcggtgag gggtgcgccg gcctcttttc
     4441 cttcctgccc gcggggagcg tcgtcatcct ggacgacccc tattcgctgg agcgggcggg
     4501 aaatgagtat gtcgaggccc tagacagggc ggcggcgaag gcaagggagg agggcaggtt
     4561 ctacctcgag cacgaatcat acctgttttc cggggaagag ctgcgccgcg gcctcgagtc
     4621 cttccaggag gtcgtcttcg atgacctgga ggtcgaaaga agggaaggag gcgcctggtc
     4681 ggtccggttt gacgtggcga agccctccgg tttcagggaa gagggtgtct ctttcagaaa
     4741 gagcgaccgg ccccttcagc cg
//
LOCUS       DAOTMX010000114         4761 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_14739_length_4761_cov_3.912665, whole genome shotgun sequence.
ACCESSION   DAOTMX010000114 DAOTMX010000000
VERSION     DAOTMX010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4761
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14739_length_4761_cov_3.912665"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..1004
                     /locus_tag="P5573_04405"
     CDS             150..1004
                     /locus_tag="P5573_04405"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRT27333.1"
                     /translation="MKTGFIGLGHLGKTMAKRLLSEGVDLVVWNRTIDKAGDLGCPVA
                     DSPAATARTAEVIFLNLFDSKAVQAVLSGPEGLLAGGLKGKIVVDTTTNHFDWVPHFH
                     KSVADQGGWYLESPVLGSVIPASQGNLTILVSGDEGAFEKVKGTLEKLGKTIFYLGEP
                     SVATKMKLANNLLLGVFMTGIAEAVILGEAAGVDREKVLDIFAAGGGNSMVLNTKRAK
                     IAKEDFSAHFSSALIYKDLHYLQDFAREIRRPLFMGSMAKELFALTFREGKEGLDFSV
                     VYDVLKKK"
     gene            complement(1084..1284)
                     /locus_tag="P5573_04410"
     CDS             complement(1084..1284)
                     /locus_tag="P5573_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HRT27334.1"
                     /translation="MPEGKVKWFNEQKGFGFIEKDEGGDVFVHFSAIQAPGFKTLYEG
                     QRVSFDITEGKKGPAADNVKPI"
     gene            1606..2865
                     /locus_tag="P5573_04415"
     CDS             1606..2865
                     /locus_tag="P5573_04415"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HRT27335.1"
                     /translation="MACKLYTTKDYYEISVETLGWELTVCNALYPEGSPLRRILKKNA
                     TYGRLLSAYLRRFIPMDEVKRVIEIGGGYGFLMKDLLEENRFARVVMLDVSPYLLQKQ
                     RELLRHPCVEFLQEDFLETDPAVLEGMDLAVLNENLGDFPVLTGVGRELFSAEEPLEE
                     PLPVVREFLWRYAIQPPGTFPFNLNIGALKAVEKLCLAKTPYIFLSEHSCEASVPEPF
                     RSFVRVSAPGMPERIRLHGHDEHTVKFSHIETVSRALGYTSVRGPIADFIPFDFDERI
                     RVIMKSPFAYKDEHEVVRHFVEDLYQYEYLLLIRDEKAASAAGTSCRRCGKCCLTDFT
                     AYVTAEDLARWRREGREDILGMIEREHAVWAGDRLVSSESGKHVRGCPFLTWEGDHST
                     CTIYETRPGVCRRFRPASSTLCSLSLR"
     gene            3117..3206
                     /locus_tag="P5573_04420"
     CDS             3117..3206
                     /locus_tag="P5573_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003402602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AURKAIP1/COX24 domain-containing protein"
                     /protein_id="HRT27336.1"
                     /translation="MGSVVKKRKKKIGKHKYRKRLKSMRHRRK"
     gene            complement(3245..4051)
                     /locus_tag="P5573_04425"
     CDS             complement(3245..4051)
                     /locus_tag="P5573_04425"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRT27337.1"
                     /translation="MAYRFIKVKVEDYIATLSLARTESLNALNLDFAREIVDSVREVG
                     NRDDVRVVILESSARIFCAGLDLKDFVALGLLGAGAKAAFFFKERVQVLFDSCTVLEE
                     CRKPVIAAVHGQCVGGGLDIICACDIRLCTKDASFSLREVAVGFVADMGVLQRLPHII
                     GQGFTREMAYTARFYSAKEAERMGLVNAVYPDREALLAGAKELAAQIAANAPLAVMAT
                     KEVLNYSRTATVEEGMDRAIQKNAVFLASKDIREAAMAFAEKRKPDFKGE"
     gene            4164..>4761
                     /locus_tag="P5573_04430"
     CDS             4164..>4761
                     /locus_tag="P5573_04430"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HRT27338.1"
                     /translation="MKIGNLTLEGDVFLAPMAGITHPVFRWMVRDVGGAPGPTEMVSA
                     EGLVRDAERVRKYIRPFPGEKPFTVQLFGADPEAVAEAARMAVGHGADIIDLNAGCPV
                     RKVVKAGAGAALMKEPGRLALILRRTRDAVPVPLTLKIRSGWHDITAPAVAKMAEDMG
                     VDAVSVHARTAVQGFAGRADWTVIAGVKEAIRIPVIGNG"
BASE COUNT         1061 a         1299 c         1414 g          987 t
ORIGIN      
        1 ttacttccgc ttcaaggact cggcggcagc cccggagaag atgaaatcag ccgccgcccg
       61 tgacaggatg gaggcgttat tcaagaagca atgaacgaca gtaggtctct ttcgacgtaa
      121 aatttaccat tcacgaaagg agtcgcaaca tgaagaccgg ttttatcgga ctcgggcatc
      181 ttggaaaaac catggcgaaa cgcctgctct ccgaaggagt ggacctcgtc gtctggaaca
      241 ggacgataga taaggcgggt gacctcggtt gtcccgttgc cgactccccg gcggcaacgg
      301 caaggacggc ggaagtcatt ttcctgaacc tcttcgacag caaggcggtc caggcagtcc
      361 tttccgggcc ggaagggctg ctcgcagggg gcctcaaagg aaagatcgtc gtagacacca
      421 cgaccaacca tttcgactgg gtcccccatt ttcacaagtc cgttgccgac cagggcggct
      481 ggtacctgga atcgcccgtc ctgggcagtg tgatccccgc gtcgcagggg aatttgacga
      541 ttctggtgag cggtgacgag ggggcgttcg aaaaagtgaa ggggacgctc gaaaaactcg
      601 ggaagacgat cttctacctg ggagaacctt ctgtggccac aaagatgaaa ctggcgaaca
      661 acctcctttt gggcgtcttc atgaccggta tcgccgaagc ggtgatattg ggagaggccg
      721 ccggtgtaga cagggaaaag gtcctcgaca tatttgccgc aggtggggga aattcaatgg
      781 tactcaacac gaagcgggca aagatcgcga aagaagactt ttccgcgcac ttctcctcgg
      841 cccttatcta caaggacctc cactacctcc aggatttcgc gagggagatc cgcagacccc
      901 ttttcatggg cagcatggca aaggagctgt tcgccctgac gttccgggaa ggaaaagagg
      961 ggcttgattt ctcggtggtc tatgacgtcc tgaaaaagaa ataaggttga aggcgatgaa
     1021 atggaaaggg cgccctctgt cggaagacga agggcgccct tatggctctc taaccgctga
     1081 ggtctatatc ggttttacgt tatcggcagc cgggcctttt ttgccttcgg tgatatcgaa
     1141 actcacgcgc tgaccttcat acagggtctt gaagccgggg gcttgaatgg cactgaaatg
     1201 aacaaaaacg tcgccgcctt catctttctc gatgaacccg aagccttttt gttcattgaa
     1261 ccacttcact tttccttctg gcatcaccca catcctcctt tctcaaattt ctgtaaagtc
     1321 ggtttgcagc tgtgacagga agaaaaaacc gccggtactc aaaagagcat ggcggttacc
     1381 ctctgcactt ctatacctgt acccaacttt tactgttccc gcgttcgcaa gctgtcgtcc
     1441 gcgggatatt ctaaaagaac gtagccctta taatgcgcct accaccgcca tgtcaaggtt
     1501 tatttgcata ttttttctgc cgccggtagg gcatctttag gggaatcaga tggacaacag
     1561 cccccggttt cagctataga ttccggcggg aagcgggagc aatccatggc gtgcaagcta
     1621 tacacgacaa aggactacta tgagatctcg gtggaaacgc tcgggtggga actcaccgta
     1681 tgcaatgccc tctacccgga aggaagtccc ctgaggagaa tacttaagaa aaacgccacg
     1741 tacggacggc ttctttccgc ctaccttcgc cgtttcatac ccatggatga ggtgaagaga
     1801 gtcatcgaaa taggcggcgg atatgggttt ctcatgaagg accttctgga ggagaaccgt
     1861 ttcgcaaggg ttgtcatgct cgacgtctcc ccttacctcc tgcaaaagca acgagagctc
     1921 cttcggcacc cctgcgtcga attcctgcag gaagattttc tggagacgga cccggcggtc
     1981 ttggagggaa tggacctcgc cgttcttaac gagaatctcg gagattttcc cgtgctcacc
     2041 ggggtcggcc gggagctttt ctcggcggaa gagccgctgg aagaacctct gccggtggtg
     2101 cgcgagttcc tatggcggta cgccatacag ccgcccggga cttttccctt caacctgaac
     2161 ataggagcgc tcaaggccgt cgagaaactc tgcctcgcaa aaacacctta catcttcctg
     2221 tcagagcaca gttgcgaggc atcggtaccc gagccattcc gctccttcgt ccgcgtttca
     2281 gcgccgggaa tgcctgaaag gataaggctt cacggccacg acgagcacac ggtcaagttc
     2341 tcccacatcg agaccgtctc tcgtgccctc ggatacacgt cggtgcgggg tccgatcgcc
     2401 gacttcatac cgttcgattt cgatgaacgg ataagagtca tcatgaagtc gccctttgcg
     2461 tacaaagacg aacatgaggt tgtccgccac ttcgtcgaag acctctacca gtacgaatac
     2521 ctgctgctca tcagggatga gaaagctgca agcgcggcgg gcacgtcatg ccgccgttgc
     2581 ggcaaatgct gtctgacgga ctttaccgcc tacgtaacgg cggaagacct ggcacgctgg
     2641 cgccgtgagg gtagagagga catactcggg atgatcgaac gggaacatgc cgtatgggcg
     2701 ggcgaccgcc tcgtgtcgtc ggagagcggg aagcacgtcc gggggtgccc cttcctgacc
     2761 tgggaggggg accacagcac ctgcacgatc tacgaaacga ggcccggggt ctgccggcgt
     2821 ttccggccgg cctcgtcgac gctctgcagc ctcagtctac gataaaattg ctgaaaaggt
     2881 cgtttaatgc gcccgataag agtcgcaaaa ctctcgaggc cgaagacatg agaggtatga
     2941 gaaggcgtcg gatagaaagg gccgaaaatg cttacggcgg ataaatttcc ctgcttgaca
     3001 gcaactgcca cctgaaccta tactcacacg ccttgcccgc tgtgagggag ggaggagccg
     3061 ggtgaagaaa taaactggaa ggttttgcga tataccatct atttcggagt gttcgtatgg
     3121 ggagtgtcgt aaaaaaacgc aaaaagaaaa tcggcaaaca taaataccgc aaaaggctga
     3181 agagcatgcg gcatcggagg aaataggcct ctggagattt tccatataac cggtatccgc
     3241 tttgctattc acccttgaaa tcaggctttc tcttttccgc gaaggccatg gcggcttcgc
     3301 ggatgtcctt cgatgcgagg aagacggcat ttttctggat ggccctgtcc attccctcct
     3361 cgacggtcgc cgtgcggctg tagttgagta cctccttggt cgccatgacg gcaagggggg
     3421 cgttggcggc gatttgagcg gccagttctt ttgcgcccgc caggagagcc tcccggtcgg
     3481 ggtaaacggc attcacgagg cccatccttt cggcttcctt ggcggagtaa aagcgtgccg
     3541 tgtaggccat ctcacgggtg aagccctgtc cgatgatgtg cggcagtctc tggaggacgc
     3601 ccatgtcggc gacgaacccg acggcgacct ctctcaggga aaaggatgcg tctttcgtgc
     3661 agagccggat gtcgcaagcg cagatgatgt cgagaccgcc gcccacgcac tgaccgtgca
     3721 cggcggcgat gaccggtttc cggcactctt ccagcaccgt acaggagtcg aagaggacct
     3781 gaaccctttc cttgaaaaag aaggcggcct ttgcgccggc gccgagaagg cccagggcca
     3841 cgaagtcctt gaggtcgagg ccggcacaga agatacgggc ggagctttcg aggatgacga
     3901 ccctgacgtc gtcgcgattg ccgacctctc tgaccgagtc cactatttcc cttgcaaaat
     3961 cgaggttgag ggcgttgagg ctttccgtcc tggcgaggga cagggttgcg atgtagtctt
     4021 cgaccttcac tttgatgaat cggtaggcca tctctttctc ctccttcttt gactcgaaaa
     4081 ccgcttcctg gcaggctcaa aaaacggatt acgccctgac ctattacaga cccggggcaa
     4141 tgtcaagaat tcaaaggtca tggatgaaga tagggaatct gacgctggaa ggcgatgtct
     4201 tcctcgcacc gatggcggga atcacccatc cggttttcag gtggatggtc cgtgatgtcg
     4261 gcggagcgcc ggggccgacc gagatggtga gtgccgaagg gttggtgagg gacgcggaga
     4321 gggtcaggaa gtacattcgg ccttttcccg gtgaaaagcc attcaccgtc cagctcttcg
     4381 gcgcggaccc ggaggccgtc gcggaggccg cccggatggc agtcggtcat ggggcagaca
     4441 tcatcgacct gaacgcaggc tgcccggtca gaaaagtggt caaggcgggg gcgggcgcgg
     4501 cgctgatgaa agaacccggc agactggccc tgattctgcg gaggacgagg gacgccgtcc
     4561 cggtgcctct gacgctcaag atccggtccg ggtggcacga cataaccgcc cccgcggtcg
     4621 cgaaaatggc agaggacatg ggggtcgacg ctgtctctgt gcatgcccgt accgccgttc
     4681 aggggtttgc gggcagggct gactggacgg tcattgccgg ggtaaaggag gcgatccgca
     4741 tccctgtcat cggtaacggc g
//
LOCUS       DAOTMX010000115         4738 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_14829_length_4738_cov_4.290199, whole genome shotgun sequence.
ACCESSION   DAOTMX010000115 DAOTMX010000000
VERSION     DAOTMX010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4738
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14829_length_4738_cov_4.290199"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1100)
                     /locus_tag="P5573_04435"
     CDS             complement(<1..1100)
                     /locus_tag="P5573_04435"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRT27339.1"
                     /translation="MKISRFFSNLSISKKLLLAPLVILLFLVLLSVGAYMGLSAQKVA
                     IEDIYNNRFKGYQNCARVMSEITNVHANVYKVISWAGANYEAQKIDNLAKGQIQVIER
                     NVNFLREILKSAALTPEEKRLYQAALDQLLEYQKPALGVLDVASADLNAATMFMGVAD
                     EKFQVLNKSLEDLLALENRLSKEKYDYSLASFNRTLQIFVALFVVAVLLSLISSIMIT
                     RLISRSIHKTIEVIQKVADGDLTGKIDVGSEDELGRLAASVNAMQSKMGGAVGQSLSI
                     SQVLSESASEQAASLEETSASLDEMASMTRQTAAHTAEANTLMNAAKQAIEKANSSMN
                     ELTDSMKVIAAASEQTQHIVKSIDEIAFQTNLL"
     gene            complement(1178..1663)
                     /locus_tag="P5573_04440"
     CDS             complement(1178..1663)
                     /locus_tag="P5573_04440"
                     /inference="COORDINATES: protein motif:HMM:NF028509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HRT27340.1"
                     /translation="MEKNNKKGGSRMTRKMLITALAVVLAAGVAWAAGKATAKEAKAM
                     VEKAVAYYKANGQEKAFAEFNNPGGKFVDRDLYVWVTTMQGKVIAHGANEKLIGKDLY
                     NLKDTDGKQFIKEIVDKAKVSNNGWVDYKWTNPVSKKVEQKSIYFEKFNNLVFVCGCY
                     K"
     gene            complement(1794..3320)
                     /locus_tag="P5573_04445"
     CDS             complement(1794..3320)
                     /locus_tag="P5573_04445"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRT27341.1"
                     /translation="MNVLFDVLPVGILNINRAGFTVDCNDSFERITGLSRREIVGRPP
                     FLGWAEDALEENAALLRILRAGGSHRSLRRFPGKKSADSHRTLDLRIEEVRENGIITG
                     AVIVVQEAASVTATDRDSEKAVTEVGGLEHMEMLNRVLDQAAHDFNNLLSPLKNYPEM
                     IRLNYRDKNKVFKYLEGIETATAKMAEMNRDILILGRRGRYKRDKLDVNGLIENVLNQ
                     QALPGTVSVRRSMSAELMPVYGGAEELKRLFAHLLKSAREVLTDGDVLTIRTENYYLD
                     HSAGRYRGVAGGEYVKVTVGGGSTGMNEGALCRAYGSADGGARGMNALPLGMKVVHTV
                     LKNHQGYMDVEVLGDKETFFYVYLPILREAVAEKTRPAGSKRKERILVVDDDPGQLNL
                     IQDILSFFGYQVVAVQNGDEALAACRPSRPGVENFDLLVMDLVLHGGGDGVAVYRRIK
                     DVCPKQKSVLLSGFAPEGKVEELRKMGVGPHLRKPLNIPLLLQVVRQELDTPARAALH
                     "
     gene            3646..4359
                     /locus_tag="P5573_04450"
     CDS             3646..4359
                     /locus_tag="P5573_04450"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRT27342.1"
                     /translation="MSSLGAMLRKLNECKKHLHVGRIYACLLGFCELLEKTMRTTLLP
                     TEERQLNEDIKAFQKELQDSKNFKDVFGPVTFQDSDKETTLAFIKQLIVVEEEEILAY
                     KESQNSGAGQKTDVDENEQMVKKIILLIDRGDFDKAREIYKDNETLRSLIIKQYNRSG
                     IGYRKDGRYEDALAEYRKALMVAPEDEGLYYNMSRVHIEGGDWAAAKEAILEALKINS
                     AFKEGRDLLNYIRRQNPTA"
BASE COUNT         1104 a         1394 c         1166 g         1074 t
ORIGIN      
        1 aggagattcg tctgaaaggc aatctcgtcg atgctcttca cgatatgctg cgtctgttcg
       61 ctcgcggcgg cgatcacctt catcgagtcg gtcagctcat tcatcgagga gttggccttt
      121 tctatggcct gcttggcggc gttcatcaag gtatttgcct cagcggtgtg cgccgccgtc
      181 tgccgggtca tagaagccat ctcatccagc gacgccgagg tctcctccag cgaagccgcc
      241 tgctccgacg ccgattccga caggacctgc gagatggaca gagactgtcc gaccgcccct
      301 cccatcttcg actgcatcgc gttgacggag gcggccaggc ggccgagctc atcctccgaa
      361 cccacgtcga tcttcccggt caggtcgcca tcggccacct tctggatcac ttcgatcgtc
      421 ttgtggattg acctcgaaat caaccgagtg atcatgatgc tgctgattag ggaaagcaga
      481 acggcgacca cgaacaatgc gacgaatatc tgcagagtcc tgttgaagct cgccagggaa
      541 tagtcgtatt tttccttgct caacctattc tccagggcga gcaggtcctc caggctcttg
      601 ttcagtacct ggaatttttc gtcggccaca cccatgaaca ttgtcgcggc gttgaggtcc
      661 gccgaagcaa cgtcgaggac cccgagagcc ggcttctggt attccaacag ttggtcgagt
      721 gcggcctgat agagtctttt ctcttccgga gtcagagcgg ctgatttcag tatctctctc
      781 agaaagttga cgtttcgctc tattacctgt atctgtcctt ttgccaggtt gtctattttc
      841 tgcgcctcgt aattggcgcc cgcccaactg ataaccttgt aaacgttagc gtggacattc
      901 gtgatctcgc tcatcacccg ggcgcagttc tggtatccct tgaagcgatt gttgtatata
      961 tcctcgatgg caaccttctg ggccgaaagc cccatgtagg cgccgacgga gaggagcacg
     1021 agaaacaaca ggataaccaa cggcgccagc agcagcttct tcgatatact gagattgctg
     1081 aaaaatcggc tgatcttcat cttgcccccc tcatgtcgga gtaacgccgg ggcgggcgac
     1141 taaacacccc atctcccccg cgacttgtct tagtggttta cttgtaacac ccgcagacaa
     1201 acaccagatt gttgaacttt tcgaaataga tggatttctg ctcgaccttc ttcgaaaccg
     1261 ggtttgtcca tttataatcc acccatccgt tgttcgacac cttggccttg tcgacgatct
     1321 ccttgataaa ctgtttccca tcggtgtctt tcaggttata aaggtctttc ccgatgagct
     1381 tctcgttcgc accatgagcg atgacctttc cctgcatggt cgtcacccag acgtaaagat
     1441 cccggtcgac gaatttcccc ccggggttgt tgaattcggc gaaggccttc tcctgaccgt
     1501 ttgccttgta ataggcgacc gccttctcca ccatcgcctt cgcttccttt gccgtggctt
     1561 ttcccgccgc ccaggctacg cccgcagcca aaacaacggc caatgctgtt atcaacatct
     1621 tcctcgtcat cctacttcct ccttttttat tgtttttttc catcaaacgc gaaaacctct
     1681 gcgatgaatg cgcgcataag gactgcacaa cgaccagacc ctgccgaacc accgcaccat
     1741 gactatcggc gggaaccggg aaaaacttaa aaacaatttt cctttcccga ccgtcagtgc
     1801 aatgccgccc tcgcaggtgt gtccagttcc tgcctcacaa cctgtagaag aagcggaata
     1861 ttcaatggtt ttctcaaatg gggccccacg cccatcttgc gcagttcttc gaccttcccc
     1921 tctggtgcaa aacctgaaag cagcaccgat ttctgcttgg gacaaacatc ttttattctc
     1981 ctgtaaacag cgactccgtc cccccctccg tggagcacca ggtccatcac gaggaggtcg
     2041 aaattctcca ctccgggccg agagggccga caggctgcca gcgcttcgtc accgttttgc
     2101 acggccacga cttgatagcc aaaaaaagaa aggatatcct gtatcagatt cagttgaccc
     2161 gggtcgtcgt ccacgaccag tatcctttct ttgcgcttcg acccggcggg ccgagtcttt
     2221 tccgctacgg cttcccgcaa gatggggagg taaacataga agaatgtctc cttatccccg
     2281 aggacttcga catccatata gccctgatgg tttttgagga ctgtgtgaac gaccttcatt
     2341 ccgaggggaa gggcgttcat cccccttgcc cctccgtcgg ctgaaccgta tgcccggcac
     2401 agggcgcctt cgttcatgcc cgtactgcct cctccgacgg ttaccttcac gtattcacct
     2461 ccggccacgc cgcggtacct ccccgcagag tggtccaggt agtaattctc cgtacggatg
     2521 gtcagcacgt ccccatcagt caatacctca cgggcgctct tcagcaggtg ggcgaacaac
     2581 cttttaagtt cctcggcgcc gccataaacc ggcatgagtt ccgccgacat gcttcgtcga
     2641 acggagactg tcccgggaag agcctgttga ttcaggacgt tttcgatgag gccgttgacg
     2701 tcgagcttat cccgcttgta ccgtccccgc ctgcccagta tcaggatgtc ccggttcatc
     2761 tcggccatct ttgccgtcgc ggtttcgatc ccttcaagat acttgaagac cttgttcttg
     2821 tcccggtagt ttaggcggat catttccgga taatttttca gcggggagag taggttgttg
     2881 aaatcatgcg ccgcctgatc gaggacgcga ttcagcatct ccatatgctc gagcccccct
     2941 acctcggtaa ccgccttttc cgaatcccta tccgtcgccg tgacacttgc cgcctcctgg
     3001 acgacgataa ctgcaccggt tatgatacca ttttcccgga cctcctcaat ccgcaaatcc
     3061 agagtccggt gcgagtccgc acttttcttt cccggaaaac gccttaatga tcgatgcgac
     3121 ccgccagctc gcagtatccg caacagggcc gcattctctt ccaaggcgtc ttccgcccac
     3181 cccaaaaagg gcggacgacc gacgatttcc cgccgcgaca ggcccgttat cctttcgaag
     3241 gagtcattgc agtcaacagt gaaccctgcc ctgttgatgt tgaggatgcc gaccggcaag
     3301 acatcgaaca agacattcat gttaggcccc tgccaccgtt cagggaggtg cgcaagtgta
     3361 cggctctctt gaggactggc gatcagttgt ttcgacatcg atgtacctcc ttcggaaaat
     3421 cgactctgat aaagaggaga catctttgag gcagcatttc ccatgccacc tcacggtggg
     3481 cttgcgaagg ccaatgcaaa aagtaagagg agggacagat gcgacggacc gcttgccgaa
     3541 agtggaagga ttacgagtaa ttgaaacccg gccggaacaa cgatggccgg cattttgcaa
     3601 gcataacaag aaaatccaga gcattctact gacggagcca caaagatgag cagtctcgga
     3661 gcaatgctga ggaaactaaa cgaatgcaaa aaacatctcc acgtcggtcg gatatacgcc
     3721 tgtctcctcg ggttctgcga actcctggaa aagactatga ggacaaccct ccttccgacc
     3781 gaggaaaggc aattaaacga agatatcaag gccttccaga aagagcttca ggatagcaag
     3841 aacttcaagg acgtcttcgg cccggtcacg ttccaggata gcgacaaaga aacgacgctg
     3901 gccttcatca agcagttgat cgtcgtcgaa gaagaggaga tcctcgctta caaagaaagt
     3961 caaaattctg gcgcaggcca aaaaaccgac gtcgatgaaa acgaacagat ggtcaagaaa
     4021 atcatactcc tcatcgatag aggcgacttc gacaaggcaa gggagatata caaggataac
     4081 gaaacactcc gctcgctgat cataaaacaa tacaaccggt cgggcatcgg gtaccgcaag
     4141 gacggcaggt acgaggacgc gctcgccgaa tacaggaaag ccctgatggt ggcgcccgaa
     4201 gacgagggcc tttattacaa catgtcgcgg gtccacatcg aggggggtga ctgggccgcc
     4261 gcaaaggaag ccatcctgga ggccctgaag ataaacagcg ctttcaagga aggccgggat
     4321 ctactcaact atatccggcg gcaaaatccg accgcatagg ttgatgagag tcggaaattc
     4381 tttccctatc tttcgagcac aaccgttacc ggccgagagc aaaccacgga ctaaatcaga
     4441 aatcaccgtc gcaccggtcg ctcggacctc catcccaatc taatagcgac aagcctcagc
     4501 actcaagacc tgccgctctc gtaacggcgc gcaaattgca tttccttacg ccaggcgaca
     4561 gaaacctcaa agagccaatc caatcaggat cgaatggaca gccttatccc ttgtaatgga
     4621 aggattttcg gacagatggc aaccatcttt gatcgtgacg gccgcgagac tgcaactatg
     4681 aggcacgccg atattcttga ggaatcctca tggcaaatga aatagaagaa ctgaaaaa
//
LOCUS       DAOTMX010000116         4707 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_14954_length_4707_cov_3.702494, whole genome shotgun sequence.
ACCESSION   DAOTMX010000116 DAOTMX010000000
VERSION     DAOTMX010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4707
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14954_length_4707_cov_3.702494"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="P5573_04455"
     CDS             <1..382
                     /locus_tag="P5573_04455"
                     /inference="COORDINATES: protein motif:HMM:NF024644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRT27343.1"
                     /translation="ACVVEHSVSKNPVKAFKGIKPRPVPRNYVDEGGPLSFSVNCRHC
                     DEPLCVAVCIAGAMTKDEASGVVKHNAEKCAGCWSCIMACPYGVVRVDPGKNRVVKCD
                     LCDGRDTPACVQACPNRALVFEDR"
     gene            387..1649
                     /locus_tag="P5573_04460"
     CDS             387..1649
                     /locus_tag="P5573_04460"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRT27344.1"
                     /translation="MNYVIIGNGAAAVGAVEGIRRTDKENPVTLVSHEPYRAYGRPLI
                     SNLLSGKIKEADIYLRAENFYEVRGVRPLLGKRATGIDLEKKRVFLEDGEAVAFDRLL
                     IATGGIPFVPPIKGKDGSGVYTFTTLDDANKLAARVDKMKKAVVVGGGLIGLKAAEGL
                     NDRGVKVTVVELADRILSSAFDRQAGDIIERRLREVGLEIILNDSVAEIVRVRGRVKG
                     VILKSGERVESSDVVLAIGVVPNKAIVEGTRIITNRGVLTDEYMETNVPGVYAAGDVV
                     EPTDLLLGQRRVTPIWPNAYIQGRCAGMNMAGERKSYGGSIPMNSIEFYGVPTVSIGV
                     SNPPDASYEVRTSGDPAENVYRKIVFKDDRLVGAVLVGRIEKAGVLTGLIADRVDVGA
                     FKDELLKGDFGFVRMPVETRKRILAAVR"
     gene            1683..2798
                     /locus_tag="P5573_04465"
     CDS             1683..2798
                     /locus_tag="P5573_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase family protein"
                     /protein_id="HRT27345.1"
                     /translation="MGSWDYQKDISGCGLSGLINKKGERVSGELIARSIATQHDRGNG
                     LGGGFAAYGIYPEFKDHYALHVMCDDRRALADTETFLKDSVYVTHEERIPTRPNRKII
                     NVPILKRYFVKPRAETEGTTFDKGLSEDDFMVQVVMRVNMDIAGAFVFSSGKNMGAFK
                     GVGYPEDIADFYRLDEYSGYLWTAHNRFPTNTPGWWGGAHPFTILDWSIVHNGEISSY
                     GINKRYLEMFGYHCTLLTDTEVVAYLLDLLVRKNGLPIDVACLALAPPFWQEIDRMDE
                     GRQRLCRAIRMVYGSALLNGPFAILFAHNGGLVGLNDRIKLRPLVAATKNDTVYMASE
                     ESAIREICPDPDRVWAPKAGEPVIAELENRDGGCYGD"
     gene            2788..3528
                     /locus_tag="P5573_04470"
     CDS             2788..3528
                     /locus_tag="P5573_04470"
                     /inference="COORDINATES: protein motif:HMM:NF013645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27346.1"
                     /translation="MEIDARGVHYRELNRMIREAVAEGERRFVLKNVNGQRYIADGIG
                     EKVKIDVYGTPGQDMAAFMKGPEIIVHGNAQDGVGNTMDDGTVAVEGMAGDIVGYGMR
                     GGSIYVLGDVGYRVGIHMKAYRDKTPVIVVGGKAGDFLGEYMAGGVIILLGMFGEDRN
                     GNPPAGHFLGTGMHGGAIFVRKGVDEHRLGRGLKSVEVSPDDMETIKPYLREFARTFG
                     LGLKEILGERFYKIVPETHRPYGNMYAY"
     gene            3573..4211
                     /gene="hisH"
                     /locus_tag="P5573_04475"
     CDS             3573..4211
                     /gene="hisH"
                     /locus_tag="P5573_04475"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HRT27347.1"
                     /translation="MIAVIDYRVGNLGSVRRAVERLGFPCKVTGETDEILSAERVIFP
                     GVGAAGKAMECLRETGLDEAVRESLRLGKPILGICLGAQVIFERSEENDTLCLGLIKG
                     KAARFPEPLYGAAEGLLKVPHIGWNGVIPQRQHPVLEGIRAADEFYFVHSYYPLPEDE
                     ENVITLTDYGVRFPSVIGCRNLVAVQFHPEKSGTAGLKLLRNFCTWDGRHAE"
     gene            4201..>4707
                     /locus_tag="P5573_04480"
     CDS             4201..>4707
                     /locus_tag="P5573_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HRT27348.1"
                     /translation="MLSKRIIPCLDVREGKTTKGIKFEENIDIGDPVEMARFYYEEGA
                     DEVVFYDITASSDRRDIMIDVVSRVAREIYIPFCVGGGIRTLEDMRAVLLAGAEKVSV
                     NSAAVLNPRIIAEGAEVFGSQCIVLGMDVKKVAPSGRIPSGYEVVIDGGRTRTGRDAL
                     EWAKEAESL"
BASE COUNT         1038 a         1211 c         1563 g          895 t
ORIGIN      
        1 cgcctgtgtc gtcgagcatt ccgtgtcgaa aaaccccgtc aaggccttca aggggataaa
       61 accgcggccg gtaccgagga actacgtaga tgagggcggc cccttgagtt tttccgtgaa
      121 ttgccgtcac tgcgatgagc cgctttgtgt tgcggtctgc atagcggggg cgatgacgaa
      181 ggatgaggcg agcggcgtcg tcaaacacaa cgcggaaaaa tgcgccggat gctggtcctg
      241 catcatggcc tgtccatacg gcgtcgtcag ggtggacccg ggaaagaaca gggtcgtcaa
      301 atgcgacctc tgtgacgggc gtgatacgcc cgcttgtgtc caagcctgtc ctaacagggc
      361 tctggtgttc gaggataggt gattcaatga actatgtcat catcggaaac ggcgcggccg
      421 ccgtgggggc agtggagggc atccggcgga cggataagga gaatcccgtg acactcgtct
      481 cccatgagcc ttaccgggct tacggccgtc cgctgatttc caacctgttg agcggaaaga
      541 tcaaagaggc ggacatctac ctccgggccg agaattttta cgaagtgcgt ggcgtgagac
      601 cccttctcgg caagagggcg accgggattg acctcgaaaa gaagagggtc ttcctcgagg
      661 acggcgaggc cgttgccttc gataggctgc tcatcgccac ggggggtatc cccttcgtcc
      721 cacccatcaa gggtaaagac gggtcaggag tatacacctt caccaccctc gacgacgcga
      781 ataagctggc ggcgcgcgtg gacaagatga agaaggccgt cgtggtcggc ggcggcttga
      841 tcggcctcaa ggcggcggag ggccttaacg acaggggagt gaaggttacg gtggtcgagc
      901 ttgcggaccg tatcctgagc tccgctttcg acagacaggc gggcgacatc atcgagagac
      961 gcctcagaga ggtcggcctg gagatcatcc tcaacgactc ggtcgcagag attgtcaggg
     1021 taagggggcg cgtaaaaggc gtaatcctga agagcggcga aagggtcgaa tcgagcgatg
     1081 tcgtcctcgc gatcggagtc gtgcccaaca aggcaatcgt ggaggggacc aggataataa
     1141 cgaatcgtgg tgtgctgacg gacgagtaca tggagacgaa cgtgcccggc gtgtatgcgg
     1201 cgggagacgt ggttgaaccg acggaccttc tgctcggaca gaggagggtg acgccgatct
     1261 ggccgaatgc ctacatccag ggaaggtgcg ccgggatgaa catggccggg gagcgaaaat
     1321 cctacggcgg gagcatcccg atgaattcga tcgaatttta cggtgtcccc accgtgtcga
     1381 taggggtgtc gaatcccccc gacgcaagtt acgaggtccg cacttcgggc gacccggcgg
     1441 agaatgttta ccggaagata gtcttcaagg atgatcggtt ggtgggagca gttctggtgg
     1501 gacgcatcga aaaggcgggc gttctcacag gccttattgc cgacagggtc gacgtggggg
     1561 ccttcaagga cgaactgctg aaaggggatt tcggttttgt ccgtatgccc gtcgagacaa
     1621 ggaaacggat cctggcggcg gtgagatagt catagaatac ggttgggcaa taggagtcgg
     1681 cggtgggttc ttgggattac cagaaagaca tatcaggttg cggactttcg ggtctgatca
     1741 acaaaaaggg agagagggtg agcggtgagt tgattgcccg ctccatcgcg acgcagcatg
     1801 accgcggcaa cggactcggc gggggttttg cggcttacgg gatatacccg gaattcaagg
     1861 atcactatgc cctccatgtg atgtgcgacg acaggcgggc ccttgcggac accgaaacgt
     1921 tcctcaagga ctcggtctac gtgacacacg aggagaggat tcccacgagg ccgaaccgga
     1981 agatcatcaa cgtgcctatc ctgaagcgct atttcgtcaa gccccgggcg gagacggaag
     2041 ggacgacttt cgacaaggga ttgagcgagg acgactttat ggtacaggtc gtgatgaggg
     2101 tcaacatgga tatagcgggg gcgttcgtct tctccagcgg gaagaacatg ggcgccttca
     2161 agggtgtcgg ttatccggag gacatcgccg atttctaccg cctcgatgaa tactcgggct
     2221 atctctggac ggcccacaac cgttttccca ccaacacacc gggctggtgg ggaggggcgc
     2281 acccctttac gatcctagac tggtcgatcg tccataacgg cgagatctca tcctacggga
     2341 taaacaagcg ttacctggag atgttcggct accactgcac gctccttacg gacacggagg
     2401 tggtggccta cctgctcgac ctcctggtca ggaagaacgg cctgcccatc gacgtggcct
     2461 gcctcgccct tgcgccgcct ttctggcagg aaatcgaccg aatggacgag gggaggcaga
     2521 ggttatgcag ggctatccgg atggtctacg gaagcgccct tctcaacggc ccttttgcca
     2581 tcctctttgc ccacaacggg gggcttgtgg ggctcaacga ccggataaag ctccgccccc
     2641 ttgtggcggc gacgaagaat gatacggttt acatggcaag cgaagagtcc gccatcaggg
     2701 agatctgccc ggacccggac cgtgtttggg cgccgaaggc aggcgagccc gtgatagcgg
     2761 aactggaaaa tagggatgga ggttgttatg gagattgacg ccagaggggt ccattacaga
     2821 gaactgaaca ggatgatccg cgaggccgtg gcggagggcg agcgtcgttt cgtcttgaaa
     2881 aacgtcaacg gccaacgtta catagccgac gggatcgggg aaaaggtcaa gatcgacgtc
     2941 tacggcacac ccggacagga tatggccgcc ttcatgaaag gcccggagat cattgttcac
     3001 ggaaacgccc aggacggcgt gggaaataca atggatgacg gcacggtcgc cgtcgaaggt
     3061 atggcgggcg acatcgtcgg ctacggcatg agagggggaa gtatttatgt cctgggcgac
     3121 gtgggttatc gtgtggggat ccacatgaag gcatacaggg acaaaacacc ggtaatcgtc
     3181 gtcgggggca aggcagggga cttcctgggt gagtacatgg ccggcggtgt tatcatcctc
     3241 ctcggtatgt tcggggaaga caggaacggt aatccaccgg cgggccattt tctcgggaca
     3301 gggatgcacg ggggtgcgat cttcgtccgc aagggagtgg atgagcacag gcttggaagg
     3361 ggccttaaat ccgtcgaagt atctcctgac gacatggaga ccatcaaacc ttacttgaga
     3421 gagtttgccc ggaccttcgg cctcgggctg aaggaaatcc tcggcgagcg attctacaag
     3481 atagttccgg aaacccacag gccgtacgga aatatgtatg cctattagcg gcggttattg
     3541 aatgtggcca agattgatga taacttctct ttatgattgc cgttatcgat tatagggtcg
     3601 gaaaccttgg cagcgtccgg agggccgtag aacggttggg tttcccctgc aaggttaccg
     3661 gggagacgga tgagattctc tctgcggaga gggttatttt ccccggtgtc ggcgcagcgg
     3721 gaaaggccat ggaatgtctc agggagacgg gccttgacga agccgtccgg gagagtctcc
     3781 ggctgggaaa gcccatactg ggaatatgcc tgggggccca ggtaattttc gaaaggagcg
     3841 aggaaaacga taccctttgt ctcggcctca tcaaggggaa ggccgcgcgg tttcccgagc
     3901 cgttgtacgg tgccgcagaa ggcctgctca aggtcccgca cataggttgg aacggggtga
     3961 ttccgcagag acagcacccc gtcctcgaag ggatccgggc cgcagacgaa ttctatttcg
     4021 tccactccta ctatcccctc cccgaagacg aagaaaacgt gattactctc acggactacg
     4081 gcgtccggtt tccctcggtc atcggttgcc ggaaccttgt cgccgtccag ttccaccccg
     4141 agaagagcgg aactgcgggg cttaagctcc tcaggaattt ctgcacatgg gacgggcgcc
     4201 atgctgagta agaggatcat cccctgtctc gacgtccggg aggggaagac gaccaagggg
     4261 atcaagttcg aggagaatat agacatcggt gatcccgtgg aaatggcccg gttttactac
     4321 gaggaagggg ccgatgaggt agtcttctac gacatcacgg cgtcgagcga caggcgggac
     4381 atcatgatag acgtggtgag ccgggtcgcc cgggaaatct acatcccctt ttgcgtcggc
     4441 ggaggcatcc ggacgcttga agacatgcgg gccgtcctcc tggcgggggc cgagaaggta
     4501 agcgtcaaca gcgccgcggt cctgaaccca cggatcatcg ccgaaggggc tgaggtcttc
     4561 ggaagccagt gcatcgtcct cgggatggat gtgaaaaaag tcgccccctc gggcaggatt
     4621 ccgtcgggct acgaggtagt catcgacggc ggccggactc gcacgggccg tgatgcgctg
     4681 gagtgggcca aagaggccga gagtctg
//
LOCUS       DAOTMX010000117         4690 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_15024_length_4690_cov_3.636893, whole genome shotgun sequence.
ACCESSION   DAOTMX010000117 DAOTMX010000000
VERSION     DAOTMX010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4690
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15024_length_4690_cov_3.636893"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1407)
                     /locus_tag="P5573_04485"
     CDS             complement(<1..1407)
                     /locus_tag="P5573_04485"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HRT27349.1"
                     /translation="MVNRIEVGFKRGVRDALGEKVRRRIVEHLHMDVEKVSTVDVFTV
                     EGDLSKGELKAIARGPLSDPVIQRYAVNRGLADDFDWLIEVGFRPGVTDNVGKTAREA
                     IELFLGESLGGRAIAVYTSRQYAVSGRVGREGAERIASGLLANELIERYEVVSGRDWD
                     REKGMPAYVPRVVGEGIICVGEIDLQVGDDELLAISSERLLALNLEEMKAVRRYFQDA
                     AVVEERRRFGLGEYATDVELEALAQTWSEHCKHKIFNSRITYLEEDGGEGVVIDSLFQ
                     TYIKRATQEIREKMGKDDWCLSVFVDNAGVIKFNDDYSLVFKVETHNSPSALDPYGGA
                     LTGIVGVNRDPFGTGKGAKLIFNTDVFCFAPPFYDKPLPKRILHPRRIYEGVREGVEH
                     GGNKSGIPTINGCIVFDERFLGKPLVYCGTGGIMPARLHGRPTHTKEVRPGDLIVMTG
                     GRIGKDGIHGATFSSEELH"
     gene            complement(1391..2773)
                     /locus_tag="P5573_04490"
     CDS             complement(1391..2773)
                     /locus_tag="P5573_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRT27350.1"
                     /translation="MEKILVVDDEANMRLVLSALLKKGGYEVVEAENGLEALRVLKAG
                     DVDVVVTDLRMPKLDGMGLLEEVVEDYPSTPVIMITAHGTVATAVDAMKKGAFDYITK
                     PFDQNELKRVIRKALKTSALNEEELVVGPGEIDRYGIIGSSKPMLEVYEAVKRVAPTT
                     TTVLLTGETGTGKDLIAQAIHRNSPRKDNPFIKINCAAIAETLIESELFGYEKGAFTG
                     AGTTKPGRFELAHKGTLFLDEAGDIPKDMQVKLLRVLEDQEFERVGGLKTIKVDVRLV
                     AATNRNLLQDVKEGRFREDLFYRLNVFPIRLPPLRERREDIVPLAEYFLEKFNGKHGR
                     SVRHIDNRVKELLVGYDWPGNVRELEHMMERVVLMAKDDTVTPEDVPEEVRFRQAVPP
                     DKTEKPFKEFIKSRTEEVEKQLIVRVLEECGGNVTRAAQQLGFSRKGLQLKMIKYNLR
                     KRQENHGQQD"
     gene            complement(2868..4439)
                     /locus_tag="P5573_04495"
     CDS             complement(2868..4439)
                     /locus_tag="P5573_04495"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRT27351.1"
                     /translation="MSRLRNREGVPTPKVDAVMETFALAAISFTIAVALLIRKKKNPL
                     QITFAFLCLALFAQEAGKFFSDLSPSAFFRGLYYLGAFSVPPLTVAFSRLFMGKGFLP
                     RRVVVLATAGSAAILAAFLFPRTAPYGDRLLFFYTALVLSGCYLALLLFIRGKAVGTE
                     RKRMTYAALACGAAAVIGMTDLLAYFGYGGPRLSEMATAGLLYFFLLTITHPDLPELY
                     ELMARAFIVLVMILFATAVFFVVDGLFGKGPMPEFAIVVMAALLIVIALDPLRQVLRK
                     AFEYFFPESKDFFTSIYGFDEELEREKAALLEEMATGLAHEIRNPLGSIKGAAQYLQS
                     EVEGTESRKLLDVIIEETDRLNRVVSQFLDYAKPFRLDLKKQDINPVIEKAVSLIRAN
                     HAAEGIVVETDLNPNLPEVEIDAEQMIQVILNISFNAIEAMPEGGTLLFRTSRIESEK
                     GDAVGIAIRDTGRGMDKEEMQKIFKPFFTTKERGIGLGLAICRRIVKNHGGYIRVKSI
                     PGQGTIFYIRLDVSA"
BASE COUNT          851 a         1514 c         1323 g         1002 t
ORIGIN      
        1 gtgcagctct tccgacgaaa aggtggcgcc gtggatcccg tctttcccga tcctccctcc
       61 cgtcatcacg atcaggtctc ccggccggac ttccttcgtg tgcgtgggcc tcccgtggag
      121 gcgggccggc atgatcccgc ccgtcccgca gtatacgagg ggcttcccga gaaacctttc
      181 gtcgaagacg atgcagccgt ttatcgtggg gatgccgctc ttgttgccgc cgtgctccac
      241 gccctccctt acgccttcgt agatgcgccg cggatggaga atcctcttcg ggagcggctt
      301 gtcgtagaaa ggcggggcga agcagaagac gtcggtgttg aagatcagtt ttgccccctt
      361 gccggtcccg aaggggtccc ggttgacccc gacgatcccc gtgagggcgc ccccgtaagg
      421 gtcgagggcg gagggagagt tatgggtctc caccttgaag acaaggctgt agtcgtcgtt
      481 gaacttgatg accccggcgt tgtcgacgaa cacggagagg caccagtcgt ctttccccat
      541 cttctcccgg atctcctgcg tcgctctctt gatgtacgtt tggaaaaggg agtcgatgac
      601 gacgccctcc cctccatctt cttccaggta ggtgatccgg ctgttgaata tcttgtgctt
      661 gcagtgctcc gaccaggtct gggccagggc ctccaactcg acatcggtcg cgtattcacc
      721 gaggccgaac cgccgccgct cctcgacgac cgcggcgtcc tggaaataac gccggacggc
      781 cttcatctct tcgaggttca gggcgagaag cctttcgctg ctgattgcga ggagctcgtc
      841 gtcgcccacc tggaggtcga tttccccgac gcagatgatg ccctcaccga cgaccctcgg
      901 gacataggcg ggcatccctt tctcccggtc ccagtccctc ccgctcacga cctcatagcg
      961 ttcgatgagt tcgttggcga ggagccccga ggcgatcctt tccgccccct ccctaccgac
     1021 ccggccgctc acggcatact gccgcgaggt gtagacggcg atggcccttc ccccgagact
     1081 ctctcccagg aaaagctcga ttgcctcccg ggcggtcttg cccacgttgt cggtcacgcc
     1141 cggccggaag ccgacctcga tcagccagtc gaaatcatcc gcgagccccc tgtttacggc
     1201 gtaacgctgg atgacggggt ccgaaagcgg gccgcgggct atcgccttca gctcaccctt
     1261 gctgaggtcg ccttcgaccg tgaagacgtc gaccgtggag accttctcga cgtccatgtg
     1321 gaggtgctcg acgatccgcc tgcggacctt ctcgcccaag gcgtctctga cccccctctt
     1381 gaacccgacc tcaatcctgt tgaccatggt tttcctgccg cttgcgaagg ttatacttga
     1441 tcatcttcag ttgaaggccc ttgcggctga agccgagctg ctgcgccgcc cgcgtcacgt
     1501 tgccgccgca ttcctccagc acccggacga tcagctgctt ctccacctct tccgtccggc
     1561 ttttgatgaa ttccttgaag ggtttctccg tcttatccgg ggggacggcc tgccgaaacc
     1621 gcacctcctc cgggacatcc tccggcgtca ctgtgtcgtc cttcgccatc aggacgaccc
     1681 gctccatcat gtgctcgagt tcccgcacgt tccccggcca gtcgtacccc acgaggagtt
     1741 ccttcacccg gttgtctatg tgacggaccg accgcccgtg cttgccgttg aacttctcga
     1801 gaaaatactc ggcgagcggg acaatgtcct cccgcctctc ccgcaggggg ggcagacgga
     1861 tgggaaagac gttcaaccgg taaaagaggt cctcccggaa cctcccttcc ttcacgtcct
     1921 gaaggaggtt tctgttggtg gccgccacca gccggacgtc gaccttgatc gtcttcagcc
     1981 cccctacccg ctcgaactcc tggtcttcaa ggacccgcaa cagcttgacc tgcatgtcct
     2041 tcggtatgtc ccccgcctcg tcgaggaaga gggtgccctt gtgggcaagt tcgaaccgtc
     2101 ccggtttggt cgtcccggcg cccgtgaaag cccccttctc gtaaccgaag agctcgcttt
     2161 cgatcaacgt ctcggcgatg gctgcacagt tgatcttgat gaaggggttg tctttcctcg
     2221 ggctgttcct gtggatagcc tgggcgatga ggtctttccc cgtacccgtc tcccccgtca
     2281 ggagtaccgt cgtcgtcgtc ggggccaccc gtttgacggc ctcgtagacc tccagcatgg
     2341 gtttgctcga cccgatgatc ccgtaccggt cgatctcgcc ggggccgacg accagttctt
     2401 cctcgttcag ggcgctcgtc ttcagggcct tccggatcac ccttttcagc tcattctggt
     2461 cgaagggctt ggtgatgtag tcgaaggcgc ccttcttcat agcgtccacg gccgtcgcca
     2521 cggtcccgtg ggccgtgatc atgatgacgg gcgtcgaggg ataatcttcg accacctctt
     2581 ccaggagacc catcccgtcg agtttgggca tccgcaggtc cgtcacgact acgtccacgt
     2641 cccccgcctt caatacccgg agggcctcca ggccgttctc ggcctccacg acctcgtaac
     2701 cccccttctt cagcagggcc gagaggacca gcctcatgtt cgcttcgtcg tcgacgacca
     2761 aaatcttttc catgggcgga aactacacga attccccttt cgatgcaacc atgacgaact
     2821 tatggggatg attgcagatt acacgacacc aatccccgcc tcaggcctca cgcggagaca
     2881 tcgagccgga tgtagaagat ggtgccctgc ccggggatcg attttacgcg gatgtagccg
     2941 ccgtgattct tcactatccg ccgacagatg gcgagcccca gcccgatccc cctttccttg
     3001 gtcgtgaaaa agggcttgaa tatcttctgc atctcttcct tgtccatgcc tctccccgtg
     3061 tcccggatgg cgatgccgac ggcatcgcct ttctcgctct cgatcctcga cgtcctgaag
     3121 aggagcgttc cgccctccgg catggcctcg atggcgttga aggagatgtt gaggatgacc
     3181 tgaatcatct gttccgcgtc gatttccacc tccggcaggt tggggttcag gtccgtctcc
     3241 acgacgatcc cctcagcggc gtgattcgcg cggatgagag agacggcctt ctcgatgacc
     3301 gggttgatgt cctgcttctt gaggtcgagc cggaagggtt tcgcatagtc gaggaactga
     3361 gaaaccaccc tgttcaggcg gtccgtctcc tcgatgatga catccaggag cttcctgctc
     3421 tccgtcccct cgacctcgga ttggaggtac tgcgccgccc ctttgatgga accgagggga
     3481 ttgcggatct catgggcgag gcccgtggcc atctcctcca agagggccgc cttctccctc
     3541 tccaactcct cgtcaaaccc gtagatggag gtgaaaaaat ccttgctctc ggggaaaaag
     3601 tactcaaatg ccttcctcag tacctgtctc agggggtcga gggcgatgac gatgaggagt
     3661 gccgccataa cgacgatcgc gaactcaggc atcggtcctt tcccgaacag gccgtcaacg
     3721 acgaagaaaa cggccgtcgc gaagaggatc atgaccaaga cgatgaaggc ccttgccatc
     3781 agttcgtaaa gctcgggcag gtccgggtgg gttatggtga gcaaaaagaa gtagagaagt
     3841 cccgccgtgg ccatctcgga caggcgcggc ccgccgtagc cgaaataggc gaggaggtcc
     3901 gtcatgccga tgaccgccgc tgcgccgcag gccagggccg cataggtcat ccgttttctc
     3961 tccgtcccga cggccttgcc tctgatgaaa agaaggaggg cgaggtagca gcccgaaaga
     4021 accagcgccg tgtagaagaa aaggaggcgg tctccgtaag gcgcggttcg gggaaacaga
     4081 aatgcggcca ggatcgcggc gctccccgcc gtcgccaaaa cgacgacccg ccggggcagg
     4141 aagcctttac ccatgaacaa ccggctgaag gcgactgtca gcggagggac cgaaaaagcg
     4201 ccgaggtaat acaggcccct gaaaaaggcc gagggggaga ggtccgaaaa gaacttgccc
     4261 gcctcctgcg caaagagggc gagacataag aaggcaaagg tgatctgcag ggggtttttc
     4321 ttcttgcgga tgagaagggc gacggcgatt gtaaagctga tcgccgccag ggcgaaagtt
     4381 tccatcacgg catcaacctt cggcgtcgga acgccttccc gatttctcag ccgggacata
     4441 ggggattatg cgcattttct cgtgtctccc ggaaaccgtc tttgccctga ccatgcaggt
     4501 gggttcgttt tcaaaaagtc agcctacaat accgtcaacc gccgtacttt tcaacgccat
     4561 ttcctctttc tcgccctgac gggcaccttt ccccagcacc ttaccctcct tttgatcccc
     4621 ccgaaaaaaa tcctcaaccc aagttcatga ttttcgtgtt ggtttttagg tagatttaca
     4681 tcatttaaag
//
LOCUS       DAOTMX010000118         4688 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_15033_length_4688_cov_3.303043, whole genome shotgun sequence.
ACCESSION   DAOTMX010000118 DAOTMX010000000
VERSION     DAOTMX010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4688
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15033_length_4688_cov_3.303043"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="P5573_04500"
     CDS             <1..769
                     /locus_tag="P5573_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRT27352.1"
                     /translation="AGLLDRKSSLVATRPFRAPHHTISDAGLVGGGQTPRPGEISLAH
                     NGVLFLDELPEFKKNVLEVLRQPLEDGRVTITRSSVTATFPSRFMLVAAMNPCPCGYY
                     TDPRRACRCTSQQIRQYRGRISGPLLDRIDIHIEVPAVRYREMVGKGHSEASARVRER
                     VNLARAIQAGRFSGQGTLCNARMSARQLKALCPIDEESQKLMEMAMERLGLSARAYTR
                     VLKVARTIADLAGEEDIRPPHVSEAIQYRSLDRGVGL"
     gene            825..1883
                     /locus_tag="P5573_04505"
     CDS             825..1883
                     /locus_tag="P5573_04505"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HRT27353.1"
                     /translation="MTAVDELAEGVVAALEAFERARRDYFPGIVPRLREAFAPVIDRL
                     KESITAHRSLSADPAGGIGKTLLDAADLCCEALRLSVEADWMEQSLVDFRKAGRRICR
                     VQERLYPLCPVLPPINRFFLEEKVRDRAETFIRDNGTTSSTAGIVHLGLDDDPYARGG
                     LSLYIPEYDDGTRPLPLVVALHGGFGHGRDFLWTWLREARSRCFIVAAPSSRGPTWTI
                     TGHDFDALLLQEVLETLCRGWNIDRKRILITGLSDGGTYALVRAMDEDPPFSAYAVVS
                     GILPPFDLRHVKGRRIYWVHGAKDWMFPVWRAKTGEKELATAGADVTLKIVPDLYHAY
                     PREQNDGILAWFDPALAL"
     gene            complement(1885..3252)
                     /locus_tag="P5573_04510"
     CDS             complement(1885..3252)
                     /locus_tag="P5573_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRT27354.1"
                     /translation="MKEKSKNTVLVVDDDLAHRTMLKTLLASWGYDVSLADDGAAAVE
                     EVRRRPFDLVLMDVRMVKVSGLEALEEIKGFNPAIPIVIMTAYASVETAVEALKKGAY
                     DYLTKPFDFDELRIVMEHAMEHSRLREENRLLRESLGKHFDKQNLIGRSRAMVSLLET
                     VAQVAPSEATVLITGESGTGKEMIAGAIHFNSPRKEGPFVKINCAAITETLLESELFG
                     HEKGAFTGADRRKEGKFRQADGGTLFLDEVSEMSLAMQVKLLRVLQEREVTRVGGSDV
                     IKVDVRVLAATNRDLIAEIRSGRFREDLYYRLNVVTLHVPPLRERREDIPLLAQHFLE
                     VFSAKNRKAIKGFTPQAMDRLLRHDWPGNIRELMNTIERAIVLARSDYLDETLIAPTS
                     REPGGDHGSKGDVVQEEGSLEEVEKATILRTLEQAGGNKSEAARRLRITRRTLHQKLK
                     KYGMM"
     gene            complement(3281..>4688)
                     /locus_tag="P5573_04515"
     CDS             complement(3281..>4688)
                     /locus_tag="P5573_04515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRT27355.1"
                     /translation="AEQPGVDYLILTDKKGVILADSDPSMIGESYGADIDLKNGPAKQ
                     TLWRRVPNPEGADTFEVYRPLASPEGEEWLIFVGLDMSPVLAAQRQDTRNIVLMAATL
                     LLIGFAGIFSLFLAQGYRSARISLSRVKAFSDGLVENLPIGLVAVDQRNHITAFNQTA
                     ETILGRDAKDVLGKSADEVLPDACKTVLRDLEAGGGVIEREMDCTVGAGAIPLEVIAA
                     TLYDDDGGHIGKVVLFRDMTELRRLKEEVERNRRLASVGSLAAGVAHEVRNPLSSIKG
                     FATYLKGKYDDEEDREVADIVVREVDRLNRVVGQLLELSRPAELKKKRQEVGAVILHS
                     LKVVEGQAKEKGIAVQADLPPGVPAVMMDPDGIEQVLLNLYLNALEAMGRGGVLSVSL
                     RKDSDRRVRVEVSDTGPGIGKDEVSRIFDPYFTTKPSGTGLGLAVVYKVVEAHGGELQ
                     VESVPGKGTRVSILLPAD"
BASE COUNT          824 a         1560 c         1387 g          917 t
ORIGIN      
        1 cgcggggcta ctcgacagga aaagttccct cgtcgcgacc aggcctttcc gggcgccgca
       61 ccacacgatc tccgacgccg gcctcgtggg cggagggcag acaccgagac cgggggagat
      121 aagcctcgcc cacaacgggg tcctctttct cgacgagctg cccgaattca aaaaaaacgt
      181 cctcgaggta ttgcggcaac cgctcgaaga cggacgggtt acgatcaccc gctcatcggt
      241 cacggcgacc ttcccgtccc ggttcatgct ggtggcggcc atgaacccct gtccctgcgg
      301 ctactacacg gaccctcgtc gggcctgccg ctgcacgtcg cagcagatcc ggcaatacag
      361 ggggcgcatc tcgggacctc tgctcgaccg gatcgacatc catatcgaag tcccggccgt
      421 ccgctaccgc gagatggtcg ggaaaggcca ttcggaggcg tccgccaggg tacgggaaag
      481 ggtcaatctg gcgcgggcca tccaggccgg acgattctcc gggcagggca ctctctgcaa
      541 tgcccggatg tcggcgcgcc agttgaaagc cctctgcccg atagacgaag agtcacaaaa
      601 gctgatggag atggccatgg aaaggctggg tctgagcgcc cgggcctaca cgagggtcct
      661 caaggtcgcc cggacgatcg ccgaccttgc gggcgaagaa gacatccgtc cccctcatgt
      721 ctccgaggcc atccagtacc gcagcctcga ccggggcgtg ggcctgtgac gcaatgcctt
      781 ccacagaccg aactatgtaa cctggagtct ggggtcgaag acagatgacc gccgtcgatg
      841 agctcgcaga aggcgtcgtc gcggcgctcg aagccttcga aagggcaagg cgcgattact
      901 tcccgggaat cgtcccccgc ctgcgcgagg cctttgcccc ggtcatcgac cgcttgaaag
      961 aatctatcac tgcacacagg tcgttgtcgg ctgaccctgc cggaggaata ggcaaaaccc
     1021 ttctcgacgc cgccgaccta tgctgcgagg ccctccggtt gtccgtcgag gccgactgga
     1081 tggagcagtc tctcgtcgat ttcaggaagg ccggacgcag aatctgccgg gtccaggaac
     1141 ggctctatcc cctatgcccg gtgctcccgc cgatcaaccg tttctttctc gaagagaagg
     1201 tccgcgacag ggccgaaacg ttcatccggg acaatgggac gacatcctca accgccggca
     1261 tcgttcatct cggcctggac gatgacccgt acgcccgggg cggcctttcc ctgtacatcc
     1321 ctgagtacga cgacggcacg aggccgctgc ctttggtagt cgcccttcac ggagggttcg
     1381 gccacggcag ggatttcctc tggacatggc tgcgggaggc gaggagccgc tgcttcatcg
     1441 tggccgcgcc cagctccagg ggaccgacct ggacgatcac cggtcacgac ttcgacgccc
     1501 tgctcctcca agaggtcctc gaaaccctct gccggggctg gaacatagac aggaaacgaa
     1561 tcctgataac cggattgtcg gacggcggga cttatgccct cgtccgcgcc atggacgaag
     1621 acccgccctt ttccgcttac gccgtcgtct ccggcatcct cccgcccttt gacctgcgcc
     1681 acgtgaaggg caggcgcatc tactgggtcc acggggcgaa ggactggatg ttccccgtct
     1741 ggcgggcaaa gacaggagag aaggagcttg ccaccgcagg ggccgacgtg accctgaaaa
     1801 tcgtccccga cctgtatcac gcctatccgc gggagcagaa cgacggtata ctcgcctggt
     1861 tcgacccggc gctagcgcta tgattcacat catgccgtat ttcttgagtt tttgatgtag
     1921 ggtgcggcgg gtgatccgga ggcgccgggc ggcctcgctc ttgtttccgc ccgcctgctc
     1981 aagggtccgc aggatggtcg ccttctcgac ttcctcgagc gacccctcct cctgaacaac
     2041 atcacccttc gacccgtggt ctcctcctgg ttctcgggag gtgggggcga taagcgtctc
     2101 gtccagataa tcggaccggg caagaacgat ggcccgttcg atggtattca tcagctcacg
     2161 gatgttccca ggccaatcat ggcggaggag gcggtccatc gcctggggag tgaacccctt
     2221 gatggccttc ctgttctttg cggaaaagac ctcgaggaaa tgctgggcaa ggagcgggat
     2281 gtcctcgcgt cgttcccgga ggggaggcac gtgaagggta accacgttca gccggtagta
     2341 caagtcctcc cggaagcgac cggaccgtat ctcggcgatg aggtcccggt tcgtcgcggc
     2401 aaggacccgc acgtcgacct ttatcacatc gctcccgccg acccgcgtga cctccctttc
     2461 ctgcaggacc ctcaacagct tgacctgcat ggcgagggac atctcgctca cctcatcaag
     2521 aaaaagggtg cccccgtccg cctggcggaa ctttccttcc ttgcggcggt cggcgccggt
     2581 gaaggcccct ttctcatggc cgaacagctc tgactcgagg agcgtctctg tgatcgcggc
     2641 gcagttgatc ttgacgaagg gaccttcctt ccgcgggctg ttgaaatgga tcgccccggc
     2701 gatcatctcc ttgcccgtcc ccgactctcc cgtgatcagg accgtggcct ccgagggcgc
     2761 cacctgggcg accgtttcca ggagactgac catcgcccgg ctccttccga tcaggttttg
     2821 cttgtcgaaa tgtttcccca ggctttcccg gaggaggcgg ttttcctccc ttagcctgct
     2881 gtgctccatc gcatgttcca tcacgatcct caactcatcg aagtcgaagg gcttggtgag
     2941 gtaatcatag gcgccttttt tcagggcctc cacggcggtc tccacggatg cgtaagcggt
     3001 catgatgacg atgggaatcg cggggttgaa gcccttgatc tcttccaacg cctcgagacc
     3061 ggaaaccttg accatccgga cgtccatgag gacgaggtcg aagggcctcc ggcggacctc
     3121 ttcgaccgcc gccgccccgt cgtcggccag ggaaacgtca tagccccaac tcgcgagcag
     3181 tgtcttgagc atcgtgcggt gggccaggtc atcgtccact acgagtaccg tgttcttgct
     3241 tttttctttc atccttggat ctcctcgcgt ttgcctcgcc tcagtcggcc gggagcagta
     3301 tcgacactct ggttcccttc cccggcacgc tctcgacctg caactctccc ccatgggcct
     3361 ccactacctt gtacacgacg gcaagcccca gccccgtacc ggacggtttc gtcgtgaaat
     3421 aagggtcgaa gatgcgcgag acttcatcct tgccgatgcc gggccccgtg tccgaaacct
     3481 cgacccggac tcgccggtcc gagtctttgc gcagcgagac ggaaaggact ccccctcgcc
     3541 ccatggcctc gagggcgttt aggtacaggt tgagcaggac ctgttcgatc ccgtcggggt
     3601 ccatcatgac cgccggcacc ccgggcggaa ggtcggcctg aacggctatg cctttctcct
     3661 tcgcctgtcc ctctacgacc ttcagactgt ggaggatgac cgcccccacc tcttgcctct
     3721 tcttcttcag ttccgccggc cgcgaaagtt cgaggagctg cccgacgacc cggttcaacc
     3781 tatccacctc ccggaccacg atgtctgcca cctccctatc ctcctcatcg tcgtactttc
     3841 ccttcaggta ggtggcgaac cccttgatgg aactgagggg attgcggacc tcatgggcca
     3901 cccccgccgc aaggctgccg accgacgcca gccgccggtt cctttcgacc tcttctttca
     3961 gacggcggag ctccgtcatg tcgcggaaga ggaccacctt cccgatgtgg cccccgtcgt
     4021 cgtcatacag ggtcgcggcg atcacttcca acgggatcgc gccggcgccg actgtgcaat
     4081 ccatttctct ttcgatgacg ccgccccccg cctcgaggtc ccggagcacc gtcttgcacg
     4141 catcggggag gacctcgtcc gcgcttttcc ccaggacatc ctttgcgtcc cttcccagga
     4201 tcgtctccgc cgtttgattg aaggcggtga tatgattccg ttggtccacc gcgacgaggc
     4261 cgatgggcag attctcgacc aggccgtcgg aaaaggcctt gacgcgggaa agggatatcc
     4321 gggccgagcg gtagccctgt gccaggaata gggagaagat gcccgcaaaa ccgatcagga
     4381 gaagggtcgc cgccatcagg acgatattcc gggtgtcctg cctctgagcg gccagaacgg
     4441 ggctcatgtc caggccgacg aagatcagcc actcctcccc ttcgggagat gcgaggggcc
     4501 gatacacctc gaaggtgtcc gctccttcgg ggttcggcac tctccgccag agggtctgct
     4561 ttgccgggcc gtttttcagg tcgatgtcgg caccgtagga ctctccgatc atggacgggt
     4621 cgctgtccgc caggatgacg ccctttttgt ccgttagaat gaggtagtca acccccggct
     4681 gctcggcc
//
LOCUS       DAOTMX010000119         4678 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_15072_length_4678_cov_3.818516, whole genome shotgun sequence.
ACCESSION   DAOTMX010000119 DAOTMX010000000
VERSION     DAOTMX010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4678
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15072_length_4678_cov_3.818516"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="P5573_04520"
     CDS             <1..683
                     /locus_tag="P5573_04520"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRT27356.1"
                     /translation="IKDTGGDYRGKKSTREEFRDSIRYFLGDGRLVSTALVDMATYFA
                     FGVFETYLPVYLSDNGFGAGMIGFIFSLQILSVAITKPFFGRLADRIDKRIQIAVGLT
                     VIGLSMLSLPFFTDEVPVVVVSLTFGLGMSLSTVATSAYTGDIADKTKLGASMGALSS
                     VMDVGHSSGPLITGFVISGFSMRAGFAISFILCLVVALLFVVFTVLKKHRRLDENRLW
                     HKIFGVHD"
     gene            774..1775
                     /locus_tag="P5573_04525"
     CDS             774..1775
                     /locus_tag="P5573_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27357.1"
                     /translation="MKKNKNEIIKRRTKARDKAKKKRQLRISRPQIVIERPPIIEMEA
                     PKGFITVSFANAMMEYAKPLMEDDAAGIDDLNRKMELASSLWNLSTSKQRNKPDEYAR
                     WMKRVTTSVRSVLNLEGEKMDRLIEEMVERHIHLFPEEVQPKPPSMFMYMRKDASYLI
                     RPFDYSRVGFKVEESILPDEEDIRFVRKVEELDDCMRREADYDAYEELAISVENESVS
                     LFKKWLIAKGFEDDPEKYIHCIGLYITFIYRYMHDDLVLLRFVPAEYLIEFFDDFLFR
                     KVFCEPFEYTYWPPALKLFYRFLGEKGYTSLPETAHMTGALDAIEPHFLEILQERYR"
     gene            1920..3830
                     /locus_tag="P5573_04530"
     CDS             1920..3830
                     /locus_tag="P5573_04530"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRT27358.1"
                     /translation="MLLVSFVLAGCAGVKVSSMSSSDYMALRRGDILTTNELSVYTGA
                     ALHVVGIDKKRCDKGGAVCRKALMETIGLNNESRLSALAELWLQEAIKLDKPPRDSGR
                     IEPILNAYLETARHAYAYLFLTERTPSMRALEDRQTQVRDYYNFAVQQMLTTLFEYYH
                     RQPPQGTDGERGTSLQSGLWRITFRNEDVRLPNLRKIPEDLIPAASLSFAGLRNHYRR
                     DGLGAELVAVTSSRVVDMKSAMVPFSETPFPPITAVVHFPGTGLEEVLNTREAVVTVY
                     DPYNRDDVELAGIRVPLAANFTSGYGLWLARSGFAAQSLLTLIGKGEVLETPRVYLMQ
                     PYNPKRHIVIMLHGLASSPEAWINVANEVLGDETLRRNFQVWQVYYPTNIPIAFNNHA
                     IRKAITQTLEHFDPEGSAEASRNIILIGHSMGGVLARLMVSSSEDRLWEAFLAKYPLE
                     GNRLANLREKLGKYVFFEPLPQVSRAIFIAAPHKGTPFAEGTLARWVAGVVTVPFSVL
                     GRLKDVAQLIFDPGSAEPVSLTRPFNSIYNLRSSDPFIRLAADLPISSRVRYHSIIGN
                     NTPDLPLVDSSDGVVPYASAHLQGAESELVIPYGHSVQEAPQAIIEIRRIMHIHLKEI
                     EKETPGAKDSVP"
BASE COUNT         1100 a         1193 c         1262 g         1123 t
ORIGIN      
        1 ccataaaaga cacgggaggg gattaccggg gtaagaaatc gacacgggag gagtttcgtg
       61 acagcatcag gtatttcctc ggggacgggc gcctggtctc gacggccctc gtggacatgg
      121 cgacgtattt cgccttcggc gtgttcgaga cgtaccttcc tgtctacctc tccgataacg
      181 gtttcggcgc cgggatgatc ggatttatct tctcactgca aatcctttcc gttgcgatca
      241 ccaaaccgtt cttcgggaga cttgccgacc ggatcgacaa gcgcatccag atcgccgtcg
      301 gattgacagt aatcggcctt tctatgctct ccttgccttt cttcactgac gaagtaccgg
      361 ttgtagtcgt ctcactcacc tttggccttg ggatgtcatt gtcgaccgtt gcgaccagcg
      421 catacaccgg cgatatagcc gacaagacga agctgggcgc ttctatggga gcgctttcat
      481 ccgtcatgga tgtggggcat tcatcgggac cgctgataac agggttcgta atatcagggt
      541 tttccatgag agcggggttt gcaataagtt tcatcctatg cctggttgtc gcccttctct
      601 tcgtggtgtt cactgttctg aaaaaacacc ggcgactcga tgaaaacagg ctgtggcata
      661 aaatattcgg tgttcacgat tgacaaacgc ccgcctatgt gagcatttta ctgcatggta
      721 tattgaatca ttagggcctg agaggatctc aagattaagt gcaggggggc catatgaaga
      781 aaaacaaaaa tgaaatcata aagcgacgga caaaagccag ggacaaggca aaaaagaaac
      841 ggcaactccg catctcgagg ccgcaaattg tcatcgaaag gccgccgatc atagaaatgg
      901 aggcgccgaa aggttttatc accgtctcct ttgcaaatgc catgatggag tatgcaaaac
      961 ctttgatgga agatgacgcg gccggtatcg acgacctgaa tagaaagatg gaattggcat
     1021 caagcttgtg gaatctatcc accagcaaac aaaggaacaa gccggacgaa tatgccagat
     1081 ggatgaaaag ggtaacgacc agcgttcgct cagtattgaa cttagagggt gagaaaatgg
     1141 atcgtttaat cgaagagatg gtcgaacggc acattcatct ttttccggag gaggttcagc
     1201 cgaagccgcc gtccatgttt atgtacatga gaaaagacgc cagttatctc atccggccct
     1261 tcgactacag ccgggttggg ttcaaggtgg aagagagcat tttgccggac gaagaggaca
     1321 ttcgtttcgt caggaaggtc gaggaacttg acgactgtat gcggcgggaa gccgactacg
     1381 acgcctatga ggaactggcg atatccgtcg aaaacgagag tgtgtcctta ttcaagaagt
     1441 ggctgatcgc aaagggattc gaagatgacc cggaaaagta catccactgt atcggtctct
     1501 acattacctt catctatcgc tatatgcacg acgacttagt cctgcttagg tttgtacctg
     1561 ccgaatacct tatcgagttt tttgatgatt ttcttttcag aaaggtcttc tgcgaaccat
     1621 tcgagtacac gtattggccg ccggccctga aacttttcta ccgcttcctc ggtgagaaag
     1681 gttatacgtc actgccggag acggctcata tgacaggggc attggacgcc atcgagccgc
     1741 actttctgga aatcctgcaa gaaaggtatc gttaagaaac ggtctcggtc gttcccactc
     1801 ctgaggcatc ttcggcgaaa cagccgggaa gcgtcggtac atctcgaacc ttaacatcag
     1861 gagagtcggt tttgttcgac aataggcgcg ttctatgctt tggacgaatt cacttgttgg
     1921 tgctgcttgt gagtttcgtg cttgccggct gtgcgggcgt caaggtgtcg tccatgagca
     1981 gcagcgatta tatggccctt cgtcgcggcg acatactcac gacgaatgaa ctcagtgtat
     2041 atacgggtgc tgcactgcat gtggtcggca tcgataaaaa gagatgcgac aaggggggcg
     2101 ccgtttgccg gaaagccctg atggaaacca tcgggctgaa caacgagagc cgcttgtcgg
     2161 cgctcgccga actctggcta caggaagcga taaaactgga caagccgccc cgcgatagcg
     2221 ggcgtatcga acccattctt aatgcctatc tggagaccgc gcggcacgcg tacgcctacc
     2281 tgtttctgac ggagcggacg cccagtatgc gcgccctcga ggatcgccag acacaggtcc
     2341 gcgactacta caactttgcc gtgcagcaga tgttgactac attgtttgaa tattatcacc
     2401 ggcagccgcc gcagggcaca gacggcgagc gcggcacttc tctgcaatcc ggtctctggc
     2461 gcatcacgtt ccgaaacgag gacgtacgat tgcccaatct tcgcaaaatc cccgaagatt
     2521 tgatccccgc cgcctcgctt tcctttgccg gtttgcgtaa ccactatcgc cgtgatgggt
     2581 tgggagccga actggtggcc gttacctcca gtcgggtggt ggatatgaaa agcgcgatgg
     2641 tgcccttcag tgaaacaccc ttcccgccga taacggccgt ggtacatttc cccgggacgg
     2701 gtctcgaaga ggtactgaat actcgtgagg cggtcgtcac cgtttatgac ccttacaacc
     2761 gtgatgacgt cgaactcgcc gggatacgag tccctctggc ggccaacttc acctccggtt
     2821 acggcctatg gcttgcgcgg tccgggtttg cggctcagtc cctgcttacc ctgatcggca
     2881 agggagaggt gttggagacg ccgcgcgtct atctcatgca gccgtacaat ccgaagcggc
     2941 acattgtcat catgctgcat ggtttggcca gcagtcccga agcctggatc aacgtggcca
     3001 atgaggtgtt gggtgatgaa accctgcgtc ggaatttcca ggtctggcaa gtttattatc
     3061 ctacaaacat acccattgcg ttcaacaacc atgcaatccg taaagccatc acgcaaaccc
     3121 tcgagcattt tgacccggag gggtcggcgg aggcatcccg taacatcatc ttgattgggc
     3181 atagtatggg tggcgtactg gcacggttga tggtctcttc ctctgaagac cgtttgtggg
     3241 aggcctttct tgcaaaatat ccgctggaag gaaaccgctt ggcgaatctg cgggaaaaat
     3301 tgggtaaata tgtttttttc gagccgctgc cgcaggtcag ccgggcgatt tttatcgccg
     3361 ccccgcataa aggcactccc tttgcggaag gcacgctggc gcggtgggtg gcgggggttg
     3421 tcaccgtgcc gttttcggtt ctgggccgtc tcaaggatgt ggcgcaactg attttcgacc
     3481 cgggttccgc cgagcctgtg tcattgacgc ggccattcaa tagtatttac aatttaagaa
     3541 gcagcgaccc tttcatccgc ttggccgccg acctgccgat ctcgtcccgt gtgcgctacc
     3601 actcgatcat cggtaacaac acccctgatt tgccgctggt tgattccagc gacggcgtcg
     3661 tgccgtacgc gagcgctcat cttcagggtg ccgagtcgga attggtgatt ccgtatgggc
     3721 atagcgtaca ggaagcacct caggccatca tcgaaatccg ccgcatcatg catattcatc
     3781 tcaaagagat cgaaaaagag acgcccgggg cgaaagattc tgtgccatga tgattctact
     3841 ctgaagctgt cagatcggtt aatatgccct tgccgcccgg ctatgcctga gcgcctgatt
     3901 attgataact cattaggtct tctacttttt atccgtttaa caggcagggg agtagaggcg
     3961 attatgaaac aggttccaca cgcggagcta taccaggttc atccgaatcg gattaccgag
     4021 tggaagaagc agctttttga gcatgccgca gacattagga gcgacaaatt tcccttgacc
     4081 cattaatgca agctgctcga cctgagcaga tccggcgtct accagactcc ggcaccaatg
     4141 agcgcaaaag acataaagtt tatgcgccag atcgacgaga tccacatggc ctatccgttt
     4201 tacggaagcc ggaaggttcg cgatgagctt tgggctcggt ctttcctgtt gcttcggaaa
     4261 ttacttattg agacttgcat aagtaacgat tcagtgctgt gtctgaaata tgcttaattc
     4321 gcaaggttta cgagatcacc ggctgtcaca ttacttacgc aataatcaag tagctcagct
     4381 taatcgccta agtaataatt gttcagatga tatcggaatt actgtttcgg cgtgctcaga
     4441 atgaaggtcc atccttcgac gacagatccc gttacaaaaa atcgaaactt atgaccgggc
     4501 attctgcatg aataacgctt ctgccggcaa cgagtcaccg ggttcggtgc aaggtgtgag
     4561 tcaccagtcg tgaaaaaata cgtctatatt aggcttttct cccgctcaat actcgtgcca
     4621 tggtctgcaa gattgtcaat aggctgtgga ctgcacccct ggagttagac agttttgg
//
LOCUS       DAOTMX010000120         4627 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_15258_length_4627_cov_3.395013, whole genome shotgun sequence.
ACCESSION   DAOTMX010000120 DAOTMX010000000
VERSION     DAOTMX010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4627
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15258_length_4627_cov_3.395013"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..1566
                     /locus_tag="P5573_04535"
     CDS             139..1566
                     /locus_tag="P5573_04535"
                     /inference="COORDINATES: protein motif:HMM:NF012592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HRT27359.1"
                     /translation="MGARKITIEPVTRIEGHARVTIHLNDQGGVEQTFFHVDEFRGLE
                     KFCEGRPYFEMPQITQRICGICPVSHHLAAAKACDGVARVEPPRPARLLRELMHMGQL
                     VQSHGMHFFHLAAPDLVLGFDADPAIRNVFGIIKANPDLALKAVNLRRYGQRVIERLG
                     GKRIHPNLSVPGGVNAPLNPTDRDEILAEMETMMDIAKVAVDIGKNWLGANKDLASTF
                     ASFPSNYMGLVDEEGGLQLYDGEIRVKNAEGKYVAQFKPEHYLAHVAEHVEPWSFLKY
                     PYYRKMGFPEGFYRVGPLGRLNVCDKIGTPLANEEFKLFRQLNGGKPVEGSLYYHYAR
                     LIELVYALERIVQLLDDPDILSKEVRAYPEARLPGQGVGVIEAPRGTLFHDYSTDENG
                     LLTRVNLIVATGNNNWAMHTAAGMVAKACVDGNKLTEGMLNRVEAAIRCYDPCLSCST
                     HAIGKMPLRIELVAVDGTVLDSISR"
     gene            1613..2161
                     /locus_tag="P5573_04540"
     CDS             1613..2161
                     /locus_tag="P5573_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HRT27360.1"
                     /translation="MGRTLVIGYGNLDRADDGVAYFVIEALRRSLGQGALKEDDTGLE
                     ELGGEIDSVFLQQLTPDLLEVMADYDEVVFVDAHVDGKLDALHCASVVPEDAALAFTH
                     HITPAMLMAWFQALYHRKPSARIVSIKGCDFDFHRGLSSFSAELVGPAVRLILKEDMT
                     FRKSLKQCKVISKKEGNRWRTS"
     gene            2146..2646
                     /locus_tag="P5573_04545"
     CDS             2146..2646
                     /locus_tag="P5573_04545"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRT27361.1"
                     /translation="MENVIDILRHSVIFSKLTDEQLKKLAAIASIETHPAGTVLYREG
                     DPAAKFYIVIDGKVALDMQCDMGPNFPPRQVIVDVVAKGESMGWSAVVEPFIFTLSCL
                     CLDRTELVAIDAVKLRGIIDEDCEMGLAVMKAVAKMIATRLTHTRVVLVGERALGIMT
                     EHTQYA"
     gene            2761..4140
                     /locus_tag="P5573_04550"
     CDS             2761..4140
                     /locus_tag="P5573_04550"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HRT27362.1"
                     /translation="MKKRWIYPVLVIELLMPFFALARQPVEPLHVGPRYPVKETAPSP
                     LPHVRPEFEDVNYWIGRLRDPDKVLMEAEEIEKVNRAALKNDLDSLDILSLPDEIDTG
                     ILKRRLARSWARYGGSRLYDSASRPLSTVYLARLFTLTNIQGVPDHVIPLYGVVVHAT
                     NLRVFPTDDLVMDRPFDYAFDRFQAGRLDVGTAVVILHYDVDCTWCFVDTGYAWGWVR
                     PNDVAVGDKEQVIKFAGGKPLIVSGDSVSFYHDHDFGEYAFELPMGAKLPLAESVHGG
                     YRVVMPCRDGYGRLFLTDGFVRADADVHEGYLPYTMGAVLRQAFKIKDGYYSWGGLNK
                     GRDCSRFIMDVFRCFGFRMPRDSNRQALFCKRNRLELEGISDGAKVEALMRIGQKPTI
                     LYMRGHVMLFLGVVDDRIYAVHSFWEYDDEHHGVVYHVGRTVVSDLTLGKGGKETLLG
                     HLKAALPLD"
     gene            complement(4270..4542)
                     /locus_tag="P5573_04555"
     CDS             complement(4270..4542)
                     /locus_tag="P5573_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27363.1"
                     /translation="MALAMGGISGVSAMKKTELIIAIKKAKGLPVKETREKPIETVVE
                     LKHKMREIREQRDSLRKRADKKKTLHLTRKISRLKKRTRRLAKKIA"
BASE COUNT          959 a         1201 c         1370 g         1097 t
ORIGIN      
        1 caaggtggac gtttttattc ccggctgccc gcccagcgcc gacgcgcttt tctttgcctt
       61 gtcggaattg ttggccggac gtacgcccgt cattttaccc cctgagtatt taaaatatga
      121 ttagaggaga tgcgagatat gggtgctcga aaaataacca tagaaccggt gacgcggatc
      181 gagggtcatg cgcgggtgac gattcatctg aacgatcagg gtggtgtgga gcagactttt
      241 ttccatgtgg acgaattccg cggtctggag aagttctgcg aaggccgtcc gtattttgag
      301 atgccgcaga tcacacagcg gatctgcggg atatgcccgg tcagtcacca tctggcggcc
      361 gccaaggcct gtgacggtgt cgcccgtgta gaaccgccgc gacctgcgag gttgttacgc
      421 gagctgatgc acatgggtca gttagtgcag tctcacggca tgcacttctt ccatcttgcg
      481 gcccccgacc tggttctcgg gttcgacgcc gacccggcga tccggaacgt cttcggcatc
      541 atcaaggcca acccggatct ggcgctcaag gcggttaacc tacggcgtta cggccagcgg
      601 gtcatcgaac ggctgggcgg gaagcggata caccccaatt taagcgtacc gggcggcgtg
      661 aatgccccgc tgaatcccac ggaccgggac gagattttag cggaaatgga gaccatgatg
      721 gacatcgcta aggttgcggt ggacatcggc aagaactggc tgggcgccaa caaagatcta
      781 gcgtcgacct tcgcatcctt tccttcgaac tacatggggc tggtggatga ggagggaggg
      841 cttcagctct atgacggaga gatcagggtc aagaacgccg agggcaagta tgtcgcccag
      901 ttcaagcccg aacattacct ggcccatgtc gccgagcatg tggagccctg gtcattcctc
      961 aaataccctt actatcgcaa gatgggtttc ccggaagggt tctaccgtgt cggtcccctg
     1021 gggcggctga atgtctgcga caagatcgga actcccctgg cgaacgaaga gttcaaactc
     1081 ttcagacagt tgaacggcgg taagcccgtc gaaggttccc tctattacca ctacgcgaga
     1141 ttgatcgaac tcgtctacgc cctcgagcgg atagtccagt tgctggacga ccccgatatc
     1201 ttgtccaaag aggttcgggc ctatccggag gcgagactac cggggcaggg agtcggtgtg
     1261 atcgaagccc ccaggggcac tctcttccac gattacagca cggacgagaa cggcctgctg
     1321 acgcgggtga acctcatcgt ggcgacaggc aacaacaact gggcgatgca cacggccgcc
     1381 gggatggtcg cgaaggcctg cgtagacggc aacaagctta ccgaagggat gttgaacagg
     1441 gtggaagctg cgatccggtg ctacgaccca tgcctgtctt gttccacgca cgctatcggg
     1501 aagatgccgt tgagaatcga gctagtggca gtggacggga cggtcctgga cagcatctcc
     1561 cgctgaccgg gagtgtcggt gtagaaaaac ggtgggaaac cgaggctcga cgatgggccg
     1621 aactctcgtt atcggttatg gaaatttaga ccgggcggat gacggtgtcg cctatttcgt
     1681 catcgaggcc ctgcggcgtt cactgggcca gggtgcactc aaagaagatg acacggggct
     1741 cgaagagctc ggcggtgaga tagattcggt gttcctgcaa caattgacgc cggatttgct
     1801 cgaagttatg gccgattatg acgaggttgt gtttgtcgat gcccatgtgg acggtaaact
     1861 tgatgcgtta cattgtgcct ccgttgtgcc ggaagatgcg gcgctggcgt ttactcacca
     1921 tataacaccc gccatgctga tggcatggtt ccaggccctt taccatagga agccgtccgc
     1981 ccggatcgtc tccataaaag gttgtgactt tgatttccac cgcggcctct cttctttctc
     2041 tgctgagtta gtggggccgg ccgtacggct tatcttgaaa gaagacatga cctttaggaa
     2101 gagtttgaaa cagtgtaaag tcatatcaaa gaaggagggg aaccgatgga gaacgtcata
     2161 gacattttaa ggcattctgt aattttctcg aagttgaccg atgagcagct gaaaaagctt
     2221 gcggccatcg cctcgataga aacccatccg gccggaacgg ttctctatcg tgagggggat
     2281 ccggccgcca agttttacat cgtcatcgac ggtaaggtcg ctttggacat gcaatgcgat
     2341 atggggccga attttcctcc ccggcaggtc atcgttgatg ttgttgcaaa gggcgagtct
     2401 atgggatggt ctgccgtcgt cgagcctttc atattcacgc tgagttgcct ttgtttggac
     2461 cggaccgaat tggtggcgat cgacgcagtg aagttgaggg gaatcatcga cgaggattgc
     2521 gaaatgggcc tcgcggtcat gaaggctgtc gctaagatga ttgcaactcg tctgactcac
     2581 acgagagtgg ttctggtggg cgaacgtgcc ctcggtatca tgaccgaaca tactcaatac
     2641 gcataaaaag aatcaagaag ataggacaaa gaggtttggt cggataggtc aaacctcttt
     2701 gtgtatccgg gggtgtcttt ctccgcatcg ttctttattg ctatttagag gtgctttaag
     2761 atgaagaaaa gatggattta tccggtcttg gtgatcgagc tgctcatgcc ttttttcgcc
     2821 ctggcccggc agccggtgga accactgcac gtcggacccc ggtacccggt gaaagagact
     2881 gcaccttccc cgttacccca tgtaaggcct gagttcgagg atgtaaacta ctggatcggg
     2941 cgcctacggg acccggataa ggtcctgatg gaggccgagg agatcgaaaa agtcaaccgc
     3001 gctgcgttga aaaacgacct ggactccctg gacatcttgt ctctccccga cgagatagat
     3061 acgggaatct tgaaaaggag actggcgagg tcttgggctc gatacggagg gtcgagactt
     3121 tacgactcgg caagtcggcc cctttccact gtctacctgg caaggctttt tacgctgacg
     3181 aacatccagg gtgttcctga ccatgtaata cccctctacg gggttgtcgt ccatgcgaca
     3241 aacctgcgtg tctttccgac ggacgacctt gtcatggaca ggccttttga ctacgccttc
     3301 gaccgtttcc aggcggggag gctggacgtc ggaaccgccg tcgtaatcct ccattacgat
     3361 gtggactgta cgtggtgttt tgtggacacg ggatacgcgt ggggatgggt caggccgaac
     3421 gacgtggctg taggagacaa agaacaagtc atcaaatttg cgggcgggaa accacttatc
     3481 gtatccggcg acagcgtgtc tttttaccat gaccatgatt tcggggaata cgcctttgaa
     3541 ctcccgatgg gagcaaaact gccccttgcc gagagcgttc atggcgggta tagagtcgtt
     3601 atgccctgcc gtgacggtta cggacgcctg tttctaaccg acggtttcgt gcgtgccgat
     3661 gccgatgtcc atgaaggcta tcttccttac acgatgggtg ctgtcttgag acaggccttc
     3721 aagatcaagg acgggtatta cagttggggc ggtctcaata agggcaggga ttgttcgcgg
     3781 ttcatcatgg acgtcttccg ctgtttcggg tttcggatgc cgagggacag taaccggcag
     3841 gcgctttttt gcaagaggaa tcgcttggag ctggagggga tttccgacgg cgcgaaagtc
     3901 gaggccctga tgcgcatcgg acagaagccg acgatcctgt acatgcgcgg acatgtcatg
     3961 cttttcttag gggtggtcga cgacaggata tatgccgtcc atagcttctg ggaatacgac
     4021 gacgagcacc acggcgttgt gtaccatgtt ggccgtaccg tagtgtctga tctgacgttg
     4081 gggaaaggcg gaaaagagac actgctggga cacctgaagg cggcacttcc gctggactga
     4141 actcccggtg accggccttt tctcgcttgg tgtgttcatg cccgagcttt ctttgcgatt
     4201 cccgtgaagg ggcatgacgt cgggaccgaa ccaggagaag cgccgcctac tttgtaggtg
     4261 cttttcggct caggcgattt tctttgccag cctgcgggtc ctttttttca gccggctgat
     4321 tttcctcgtc aggtggagcg tcttcttctt atccgctcgc tttctcaaag agtcccgctg
     4381 ttccctgatc tctcgcatct tgtgtttcag ctcgaccacc gtctcgatgg gtttctcgcg
     4441 ggtttccttc actgggaggc cttttgcctt cttgatggcg atgatcagct ccgtcttctt
     4501 catcgctgaa acgccgctga ttccacccat ggccagggcc atctccttct actcctttat
     4561 cgtcaaatct tccagcggtt tttcgtgtgc catctcttca ccctccattc gtggttgctt
     4621 ccgtcac
//
LOCUS       DAOTMX010000121         4617 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_15305_length_4617_cov_3.666374, whole genome shotgun sequence.
ACCESSION   DAOTMX010000121 DAOTMX010000000
VERSION     DAOTMX010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4617
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15305_length_4617_cov_3.666374"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..813
                     /locus_tag="P5573_04560"
     CDS             154..813
                     /locus_tag="P5573_04560"
                     /inference="COORDINATES: protein motif:HMM:NF015961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRT27364.1"
                     /translation="MLKLLTGPVYHFLNFYAKTIRLTFEGAEVPLNHLKNGGSVLIAC
                     WHQRFFGGFYFPGVYKLEPCIMISQSKDGDFIATVVQRMGWRPVRGSSTRGGKQAMKE
                     MIEAMARLRVGAHIVDGPLGPPHVIKPGLISIAQNAGAVICPAYITYEKPMIFNSWDR
                     FMIPKPFSRVHIRFGRLTAVPESLDVGEFEKLREELESEIINGYAEADRYWVEEKKRK
                     A"
     gene            complement(817..>1615)
                     /locus_tag="P5573_04565"
     CDS             complement(817..>1615)
                     /locus_tag="P5573_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HRT27365.1"
                     /translation="TSTGTPAHFIHPVEGMHGDIGIVTKDDILLAISNSGETHELNTL
                     IGNIRDRGVPLIAFTGNPESTLARRSDIVIDVGVDREACPFGLAPTSSTTAALAMGDA
                     LAVALVNRRQFNEKDFYRFHPGGSLGRRLMSRVKDVMVGGDQVPTVMPGTPAIAAVEE
                     IDRKNLGFVLIVDGERRLQGILTDGDIRRCVKRGEDFGNRTVDELMTRSPKTVDENLS
                     LAETLESMERDEITTLVVVDDERRVKGYIHLHDILGRGGTVRMSVSP"
     assembly_gap    1616..1625
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1626..1814)
                     /locus_tag="P5573_04570"
     CDS             complement(<1626..1814)
                     /locus_tag="P5573_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HRT27366.1"
                     /translation="MDKDTLEQAKEVLRVEADSILSLRERIDRNFTMAVDLIYNSKGR
                     VIVSGIGKSGLIGKKIVAT"
     gene            complement(1941..2630)
                     /locus_tag="P5573_04575"
     CDS             complement(1941..2630)
                     /locus_tag="P5573_04575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HRT27367.1"
                     /translation="MNIGALQKFSLIEYPGKICAIVFTQGCNFRCPYCHNRELVDPEA
                     YGPTITESDVFSFLEKRKKKLDAVTVTGGEPTLQPDIFSFLEKLKTMGYLTKVDSNGS
                     HPEVLKKLIDSRLVDYIALDVKAPLARYREITRSGIREEDIRESIECVMSSGVDYEFR
                     TTVVPCLLSPDDLTDIARTISGARRFALQRFVPSKTLDPAFLNERNYPEETFASIADV
                     IRPFVSELIVR"
     gene            complement(2635..>4617)
                     /locus_tag="P5573_04580"
     CDS             complement(2635..>4617)
                     /locus_tag="P5573_04580"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HRT27368.1"
                     /translation="AVARKLTIKVLAVAERLFDGSVSTVEEIQDIVEEVLLSSIYRKT
                     AKAYIIYRDQHARLRELANKMEVDLVDQYLKKTDWKINENSNMDYSLQGLNNYISSEL
                     SKVYWLNEIYPPEVKKAHVEGDFHIHDLSLLSVYCVGWDLSDLLVEGFKGASGKVESR
                     PAKHLRSALGQVVNFFYTLQGEAAGAQAFSNFDTLLAPFIRYDGLSYREIKQALQEFI
                     FNINVPTRVGFQTPFTNVTLDLTVPRYFADRDVIIGGKPQDAQYRDFQEEMDLFNKAF
                     LEVMSEGDAKGRVFTFPIPTYNITRDFNWDNRNMDHLWEMTAKYGVPYFSNFVNSDMH
                     PEDARSMCCRLRIDNRELVSRGGGLFGSNPQTGSIGVITINMPRIGYLSKDEGDFFNR
                     LDRLMLLAKESLEIKRKVLEKFTEGNLYPYTKYYLRSVKQRFDSYWKNHFSTVGLVGM
                     NEACMNLLGKTIGDPEGKAFAMKVLDHMKGRLLEFQEETGNNYNLEATPAEGTSYRLA
                     KIDREKYADMVFANGKGNGDGPPEVFYTNSTHLPVNFTDDIFEALDLQDDIQVKYTGG
                     TVFHGFIGERIPNAEAIKHFVRRICENYRLPYFTLTPTFSVCTTHGYLSGEHETCPNC
                     STPCEVFSRVVGYLRPVKQWNKGKQAEFRLRKVFTV"
BASE COUNT          979 a         1226 c         1281 g         1121 t
ORIGIN      
        1 agtgctacct tcacacggta gaagtcactg gttcaaatcc agtaccgcct accaagaaag
       61 atacgaaggg gctcacgatt tcgcgagccc ctttcctgtt ttttctcaga gattcgactc
      121 atggttacgg gaaaagatat caaatttttt ttgatgctga agctgttgac gggcccagtc
      181 tatcatttcc taaacttcta tgcgaagacg atcagactaa cctttgaagg ggcggaggtt
      241 ccgctgaacc accttaaaaa cggcgggtcc gtgttgattg cctgctggca tcagcggttc
      301 ttcggcggct tctattttcc cggggtatac aaacttgagc cctgcataat gatcagtcag
      361 agtaaggacg gggatttcat cgctactgta gttcaacgca tggggtggag gccggtccgg
      421 gggtcgagta caaggggcgg aaagcaggcg atgaaggaga tgatagaggc catggcgagg
      481 ctgagggtcg gcgcccacat cgttgacggg ccgctcggcc ccccccatgt gatcaaaccg
      541 gggttgatat cgatcgcgca aaatgcggga gcagtcatct gcccagccta cattacctat
      601 gagaaaccca tgattttcaa cagctgggac cggttcatga tccctaaacc attcagcagg
      661 gtccacatcc ggttcgggag gcttacggcg gttcccgaaa gcctcgacgt cggcgaattc
      721 gagaaactcc gggaagaact agaatcggag atcatcaacg ggtacgccga ggccgaccgt
      781 tactgggttg aagagaaaaa gaggaaggct tgagcttcag ggggacaccg acatcctgac
      841 cgtaccgccc cgcccgagga tgtcatggag gtggatgtag cccttcacgc gccgttcatc
      901 atcgacgacc acgagtgtag tgatttcgtc tctctccatc gactcaagcg tttctgcaag
      961 agaaagattt tcatcgaccg tcttcggcga tcgggtcatc agttcgtcga cggttctgtt
     1021 cccaaagtcc tctccccgtt tgacgcatcg ccgaatatcg ccgtccgtaa gtatcccctg
     1081 caatcgtctc tcgccgtcca cgatgaggac gaatccgagg ttttttctgt cgatttcttc
     1141 cacggcggcg atggccggag tcccgggcat aaccgtgggc acctggtccc cgccgaccat
     1201 gacgtctttg acccgggaca tcaggcgccg tccgagcgaa cccccgggat gaaaacggta
     1261 gaagtctttt tcgttgaatt gccgcctgtt gacgagggca acggcgaggg cgtctcccat
     1321 ggccagcgcc gccgtcgtgc tcgacgtagg tgcgagcccg aaaggacatg cctccctgtc
     1381 gacgccgaca tcgatgacga tatcgctccg acgggcaagt gtcgattccg ggttccccgt
     1441 gaacgcgatt agaggcacac ccctgtcgcg gatgttgccg ataagcgtgt ttaattcatg
     1501 ggtctccccg ctgttggaaa tggcgagcag gatgtcgtct ttggtgacga taccgatgtc
     1561 tccgtgcatg ccctcgacgg gatggatgaa gtgggctggc gtgcccgtac tggtcnnnnn
     1621 nnnnnggtgg ccacaatctt tttcccgatc agtcctgatt tcccgattcc cgagacgatg
     1681 acccgtccct tggagttgta tataaggtcg acggccatgg taaagttgcg gtcgatcctt
     1741 tccctgaggc tgaggatact atccgcctca acccttaaaa cctcttttgc ctgttccagg
     1801 gtgtctttgt ccatctacct tcgaatcaaa tcaacaatgg agcattataa gtagtcgcta
     1861 ttcatcgatg ggcgaggatt accagattcg ctccggcaat acaagtcgta tttaggctac
     1921 ggaatgctcg gcgaaggggt tcaccgtacg attaactcgg agacgaatgg gcggatgacg
     1981 tcggcaatcg aggcgaatgt ttcttccggg taatttcttt cgttcaggaa agccgggtcg
     2041 agcgtcttcg aggggacaaa acgctggagt gcaaaccgtc ttgctcccga gatggttcgg
     2101 gctatatcgg tcaggtcgtc gggggagaga aggcacggaa caactgttgt tcgaaattcg
     2161 taatccacgc cggaagacat cacacactcg atgctttccc tgatatcttc ctccctgatc
     2221 ccggacctcg ttatctcgcg gtacctggcg agaggagcct ttacgtcgag ggcgatgtaa
     2281 tcgacgagac gcgagtcgat cagtttcttc aggacttcgg gatgcgaacc gttggagtcg
     2341 accttcgtca ggtagcccat cgtcttcagt ttttcgagga aagaaaagat gtccggctgc
     2401 agtgttggtt cgccgccggt aaccgtcacc gcgtcgagct tctttttcct cttttccaaa
     2461 aaagagaaga catcggattc tgttatcgtc ggtccatacg cttccgggtc gacgagttcg
     2521 cggttgtgac agtaaggaca gcggaaattg cagccctgag taaaaactat cgcgcagatc
     2581 tttcccggat actctatcag agaaaacttc tgcaatgccc cgatattcat actctcaaac
     2641 ggtaaagacc ttcctcagcc tgaattcggc ctgcttccct ttgttccatt gtttgacggg
     2701 acgaagatac cctaccacac gggaaaagac ctcacaaggg gtagagcagt tcggacatgt
     2761 ctcgtgttcg ccgctcagat agccgtgggt cgtgcatacg ctgaaggttg gcgtaagcgt
     2821 gaaatagggg agccggtaat tttcgcatat ccgacggacg aaatgtttga tggcctcggc
     2881 gttcggtatc cgttcaccga tgaacccgtg aaagaccgtt ccccccgtgt acttaacctg
     2941 gatatcgtcc tggaggtcga gggcttcgaa tatgtcgtcg gtgaaattga ccggcagatg
     3001 ggtagagttt gtgtagaaga cctcgggagg tccgtcgccg tttccctttc cgttggcgaa
     3061 aaccatgtcg gcgtacttct cccggtctat ttttgcgagg cggtacgaag ttccctccgc
     3121 tggagtcgcc tccagattgt aattgttccc cgtctcttct tgaaattcga gcaaccggcc
     3181 cttcatgtgg tcgaggacct tcatcgcgaa ggcctttccc tcggggtcgc ctatcgtctt
     3241 gcccagcaga ttcatgcagg cctcgttcat ccctaccagg ccgacggtcg aaaaatgatt
     3301 cttccagtag ctgtcgaatc tctgctttac gctcctgagg tagtattttg tgtaggggta
     3361 gaggttccct tcggtgaact tctccaggac tttacgcttt atctccagac tctcttttgc
     3421 gagcagcatc agtctgtcaa ggcggttaaa aaaatcccct tcatccttgg atagataacc
     3481 tatccggggc atgttgatgg tgatgacgcc gatcgaccct gtctgagggt ttgagccgaa
     3541 aaggcccccg ccccgggaga cgagctcgcg gttgtcgatc cgcaggcggc aacacatgct
     3601 cctggcgtcc tcggggtgca tgtcggaatt gacgaagttc gaaaagtacg gcaccccgta
     3661 tttcgccgtc atctcccaca ggtgatccat gttgcggtta tcccagttga aatctctcgt
     3721 tatgttatac gtagggatgg ggaaggtgaa cacccgaccc ttggcgtccc cctcggacat
     3781 aacttccaaa aaggccttgt tgaagaggtc catctcttcc tggaaatccc ggtactgggc
     3841 atcctggggc tttccgccga tgatgacgtc cctgtcggca aaataacggg gcactgtcag
     3901 gtcgagcgtt acgtttgtga aaggggtctg gaaaccgacg cgggtcggca cgttgatgtt
     3961 aaagatgaat tcctgtaaag cctgcttgat ttccctgtag ctgaggccgt cgtaccggat
     4021 gaaaggggcg agcagggtgt cgaagttcga gaaggcctgg gccccggcgg cctccccctg
     4081 gagtgtgtag aagaaattga cgacctgacc aagggcgctg cgcaggtgtt tggcagggcg
     4141 gctttcgacc tttcccgacg cccccttgaa cccttccacg agaaggtccg acaaatccca
     4201 tcccacgcag tacaccgaca ggagactcag gtcgtggatg tgaaaatcgc cctcgacatg
     4261 agccttctta acctcgggag ggtagatctc attcaaccag tagaccttgc tgagctcgga
     4321 agagatgtag ttgttcaaac cctggaggga ataatccata ttgctgtttt cgtttatttt
     4381 ccagtctgtt ttctttaggt attggtcgac gaggtcgacc tccattttat tggccagttc
     4441 tctcaacctt gcgtgctggt cgcggtagat gatgtaggcc ttggcggtct tccggtagat
     4501 ggaggagagg aggacttctt ccacgatgtc ctggatttct tcgaccgtcg agacagaacc
     4561 gtcaaaaagt ctttccgcta cggcaaggac ctttatggtc agcttcctgg ccacggc
//
LOCUS       DAOTMX010000122         4614 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_15318_length_4614_cov_4.590919, whole genome shotgun sequence.
ACCESSION   DAOTMX010000122 DAOTMX010000000
VERSION     DAOTMX010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4614
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15318_length_4614_cov_4.590919"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="P5573_04585"
     CDS             <1..802
                     /locus_tag="P5573_04585"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HRT27369.1"
                     /translation="AGMERLRREILAAFIDVVRPAGIYERSDAGAREKEGLGSAEGVL
                     YGEIPEELTIEEGGVLFKVRLRESQKTGFYLDQRRNRGTVASYVGAGYRVLDVFSNTG
                     GFGLYAAAKGAGSVTLVDQSAAALEAARENVSLNGMKDVVSVKADAFAYLAREAKQGK
                     RYDLVVLDPPSFAKAKDAKGGALKGYRRLVLDGLRILKPEGYLAVFSCSHHISLDDIG
                     CACLNAAADADCRIEVLEHLFQDTDHPYILNIPQSLYLKGLLTRKTLL"
     gene            956..2437
                     /locus_tag="P5573_04590"
     CDS             956..2437
                     /locus_tag="P5573_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HRT27370.1"
                     /translation="MEFRHKSGKNFLIIWLVFLIFFFFVSLVGAVHFAFASPRTQQKY
                     RLASARDIGTAPASFADLVEKLKPAVVNISTTKIVRSEGRGQGAPFGDDFFERFFGGD
                     DFFRKFFNEAPRQYKQRSLGSGFIITRDGYILTNNHVVERADKIRVRLSSGKEYDARV
                     VGKDAKTDLALIKITPAGNLPVVEFGDSDKLRVGDWVIAIGNPFGLEQTITAGIVSAK
                     GRVIGAGPYDNFIQTDASINPGNSGGPLFSLDGKVVGINTAIVAQGHGIGFAIPINMA
                     REILPELKTKGKVTRGWLGVSVQDITEDMAQSLKIKEMEGALVAEVMPGDPADKAGIK
                     TGDVIVEVDGKKIKDTHELLRVVATIPIGKKVDVKVLRDGKAHVVPVVVTERKETMQL
                     AEKGGGTGGYFGMTVGEITPEIAQQLGLSEKRGVVVVRVKLGSPADEAGVRPRDVIVQ
                     VNKVKISSIKEYKREMSRKDARDKALLLVKRGKTNLYIALKKG"
     gene            2438..3115
                     /locus_tag="P5573_04595"
     CDS             2438..3115
                     /locus_tag="P5573_04595"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HRT27371.1"
                     /translation="MVREGSQTARLLREMYDRLERRFGDLCWWPAETPFEVIVGAILT
                     QNTNWQNVERAVANLRNENLLTEQGLLAVEEGRLAELLRPSGYYRVKAKRLKAFIHFL
                     FGEYRGDLGKMFAEDPRDLRVKLLGVKGIGEETADSILLYAGGMPVFVVDAYTRRILS
                     RHGIVDGDKSYGEIQRLFMDNLPADAPFYNQYHALLVQAGKLFCRKEPLCRDCPLREL
                     EKARSKS"
     gene            3133..3867
                     /locus_tag="P5573_04600"
     CDS             3133..3867
                     /locus_tag="P5573_04600"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRT27372.1"
                     /translation="MSLIIKELCVEIAGREVLHDIDMEINPGETHVLFGKNGSGKTSL
                     LMAIMGFSGYQITKGSITFKGRDITHLPINERANLGIGMAFQRPPTIRGLKTRDLVDL
                     LGKQRGNGGQTTKYAESLEFTEFLDREINYGLSGGEIKKSELVQLMAQNPDLILLDEP
                     ESGVDLENIALLGKVIKKLLQKNLRRLRVKSGLIITHTGLILNYVEADKGHLLLDGSL
                     KCSGNPREMFDTICKFGYEECARCQR"
     gene            3855..>4614
                     /locus_tag="P5573_04605"
     CDS             3855..>4614
                     /locus_tag="P5573_04605"
                     /inference="COORDINATES: protein motif:HMM:NF013614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HRT27373.1"
                     /translation="MSKIKEKAQAAKEKKALYGTDIDLAAFKRKAAEHAYQDDLEKIP
                     PDSQRGMTEAGIVLSEEGRAGSYIQMDHSPVHCSIREEGVEILPIVEALKRYDGLQDY
                     WWQAMAVDADKYTAHTELELHNGYFIRVSKGVKALFPVQACLYLGEEGIAQHVHNIII
                     AEEGSELHVITGCTTAPNLARALHIGVSEFYVKRGATLTFTMIHNWGEEVAVRPRTAV
                     WVEEGGTYLSHYVCMKPARSIQTDPTVRLLGEGAV"
BASE COUNT         1102 a         1275 c         1395 g          842 t
ORIGIN      
        1 ggcggggatg gagcgcctga ggagagagat ccttgccgcc ttcattgatg tggtgaggcc
       61 ggcaggtatc tacgagcggt cggatgccgg cgccagagag aaggaaggac tgggaagcgc
      121 cgagggggtc ctctacgggg agatccccga ggaactgacc atcgaagagg gcggcgtcct
      181 ctttaaggtc cggctgcgcg aaagccagaa gacgggattc tatctcgacc agaggagaaa
      241 tagagggacc gtggcgtctt acgtcggcgc agggtaccgg gtactcgacg tcttttccaa
      301 tacgggcggc ttcggcctct acgccgccgc taaaggtgcc gggtccgtta ccctcgtcga
      361 ccaatcggct gcggcccttg aggccgcccg cgagaacgtt agcctgaacg gcatgaagga
      421 cgtcgtctcc gtgaaggccg acgccttcgc ttaccttgcc cgcgaggcaa agcaaggcaa
      481 gcgctacgac ctcgtagtcc ttgacccgcc ttccttcgcg aaggcgaaag acgcgaaggg
      541 tggggcattg aaggggtata ggcgcctcgt cctcgacggt ctgcgtattc tcaagcccga
      601 ggggtatctc gcggtatttt cctgctccca ccatatatca ctcgacgaca taggatgtgc
      661 ctgcctgaac gcagcggccg acgcggactg ccggatcgag gtgctggaac acctcttcca
      721 ggataccgac cacccttaca tcctcaatat tccccagtca ctctacctga aaggactact
      781 gaccagaaaa acgcttctct gatgaatacc gataattgta ttgacttccc cgtgaaattg
      841 ataaatataa gttacatcaa gcaaagacca ctgaattggg tgcaggcggt ccccgtccag
      901 gaaaaaatcc cgacaaaaat accccctcct ggccgggacg gacagagagg atcgaatgga
      961 attcagacat aaatcaggaa aaaactttct tataatctgg ctcgtctttc tgatcttttt
     1021 cttcttcgtg tcgctggtcg gtgcggtgca ttttgccttc gcatcacccc ggacacagca
     1081 aaagtaccgg ctcgcgtcgg caagagacat cggcacggcg ccggcttcct tcgcggacct
     1141 ggtggagaaa ctgaagccgg cggtcgtcaa catctcgacc acaaagatcg tccggtccga
     1201 gggaagggga cagggcgctc ccttcgggga cgacttcttt gagcggtttt tcggcggcga
     1261 cgatttcttc cggaaatttt tcaacgaggc accgcgacag tacaagcaga ggagtctcgg
     1321 gtcgggcttt atcatcaccc gggacggtta tatccttact aacaaccacg tagtcgagcg
     1381 ggccgataaa atcagggtac gcctttccag cggtaaggaa tacgacgccc gggtcgtcgg
     1441 gaaagacgcc aagacggacc tcgccctcat caagataacg ccggccggca acctgccggt
     1501 cgttgaattc ggcgactccg acaagttgcg ggtaggggac tgggtgatcg ccatcggaaa
     1561 ccccttcggc ctcgagcaaa caatcacggc ggggatcgtc agcgccaaag gacgggtgat
     1621 cggtgccggg ccttacgaca acttcatcca aacggatgcc tcgataaacc ctggcaacag
     1681 cggtggtcct cttttttccc tggacggtaa ggtcgtgggg atcaacacgg cgatcgtcgc
     1741 ccagggacat ggtataggtt tcgccatccc gatcaacatg gcgagggaaa tccttccgga
     1801 actgaagacg aagggaaagg tgacccgcgg ttggctcggg gtctcggtcc aggacatcac
     1861 ggaggacatg gcccagagcc tgaagataaa agaaatggaa ggggccctgg tggcagaggt
     1921 gatgcccggc gacccggcag ataaggccgg gatcaagacg ggggacgtca tcgtcgaggt
     1981 ggacgggaaa aaaatcaagg acacccatga actgctccgg gtcgtcgcca cgatcccgat
     2041 agggaagaag gttgatgtca aggtcctccg ggacggaaag gcccacgtcg tccccgtcgt
     2101 cgtcacagag cgaaaagaga cgatgcaact tgccgagaaa ggcggcggca cgggcggcta
     2161 cttcgggatg acggtcgggg aaatcactcc ggagatcgcc caacaactcg gcctttccga
     2221 aaagaggggt gtcgtcgttg tgcgggtcaa gttgggaagc cccgccgatg aagctggagt
     2281 caggccccgg gacgtcatcg tccaggtgaa taaggtaaag atttcttcta tcaaggagta
     2341 taagagagag atgtcgagaa aggatgccag agacaaggcg ctgctcctcg tcaagagagg
     2401 caagacgaac ctttacatcg ctctcaagaa gggttgattg gtcagagaag ggtcgcaaac
     2461 cgcccgtctg ctgcgggaaa tgtacgacag gctggagcgc cgtttcggag acctctgctg
     2521 gtggccggcc gagacacctt ttgaagtcat cgtcggcgcg atcttgacgc agaacaccaa
     2581 ctggcagaac gtcgagaggg cggtcgcaaa cctcaggaac gagaacctcc tgaccgagca
     2641 aggcctactc gccgttgagg aaggacgcct cgcggaactc ctcaggcctt ccggctacta
     2701 ccgggttaag gcgaagaggc ttaaggcctt cattcatttc ctttttggtg agtaccgggg
     2761 tgacctcggg aagatgtttg cggaagaccc gcgggacctc agggtgaagt tgctgggcgt
     2821 caaggggatc ggcgaggaaa cggcggacag catccttctc tacgccggtg ggatgccggt
     2881 tttcgtcgtt gacgcctaca cacggcggat actctcacgc catggaatcg tcgacggaga
     2941 taaaagttac ggtgagatcc aacgcctctt catggacaat cttccggcgg acgcgccctt
     3001 ctacaatcag taccacgccc tgcttgtcca ggcgggtaag cttttctgcc gaaaagagcc
     3061 tctttgtcga gactgtccat tgcgggaact tgaaaaggcg aggtcaaaaa gctaaaaacc
     3121 gggagaagaa aaatgtctct gataatcaaa gagttgtgcg ttgaaatcgc ggggcgggaa
     3181 gtcctccacg atatcgatat ggagatcaac cccggggaaa cccacgtcct tttcgggaaa
     3241 aacggctccg ggaagacctc tctcctgatg gcgatcatgg gcttttcggg ttaccagatt
     3301 acgaagggca gcatcacctt caagggacgg gacatcaccc acctcccgat caacgaaagg
     3361 gcgaacctcg gaatcggcat ggccttccag cgaccgccca ccatcagggg actcaaaacg
     3421 agagacctgg tggacctctt gggaaagcaa agaggcaacg gcggacagac gacaaagtac
     3481 gcagaaagcc tggaattcac cgaatttctc gacagggaaa tcaattacgg cctctcggga
     3541 ggggagatca agaaatcgga gttggttcag ctcatggccc agaatcccga cctcatcctt
     3601 ctcgacgagc cggagtcggg cgtggacctc gagaacatcg cccttctcgg caaggtcata
     3661 aagaagcttc tccagaagaa cctccgccgg cttagggtca aatcgggcct catcatcacc
     3721 cacaccgggc tcatccttaa ctacgtggag gccgacaagg gtcatctcct cctcgacggt
     3781 agcctcaagt gctccgggaa cccccgggag atgttcgaca ccatctgtaa attcggctat
     3841 gaggaatgcg caagatgtca aagataaaag agaaggcaca ggcggcaaaa gaaaagaagg
     3901 ccctttacgg aaccgacatc gacctggcgg cgttcaagag aaaagcggct gaacacgcct
     3961 accaggacga cctggagaag atacccccgg acagccaacg ggggatgacc gaggcgggca
     4021 tagtcctctc ggaagaaggg cgggcgggaa gttatatcca gatggaccat tcgcccgttc
     4081 actgctctat ccgggaggag ggggtcgaga tcctgccgat cgtggaggcc ctgaagagat
     4141 acgacggctt gcaggattac tggtggcagg ccatggccgt ggatgccgac aagtatacgg
     4201 cacatacgga gctagaactc cacaacggtt acttcatccg tgtctcgaaa ggcgtgaagg
     4261 ccctcttccc tgttcaggcc tgcctctacc tcggcgagga ggggatcgcc cagcatgtcc
     4321 acaacatcat catcgccgag gagggttcgg aactccacgt catcacgggc tgcaccacgg
     4381 ccccgaacct ggcaagggca ctccacatcg gggtttcgga gttctacgtc aagcgcggtg
     4441 cgaccctcac cttcacgatg atccacaact ggggcgaaga agtggcagtc cgcccgagga
     4501 cggccgtctg ggtggaggaa ggagggacct atctctccca ttacgtctgc atgaagccgg
     4561 cgcgctctat acagacggac ccgacggtga ggctcctggg tgagggcgcc gtca
//
LOCUS       DAOTMX010000123         4605 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_15344_length_4605_cov_4.652747, whole genome shotgun sequence.
ACCESSION   DAOTMX010000123 DAOTMX010000000
VERSION     DAOTMX010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4605
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15344_length_4605_cov_4.652747"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="P5573_04610"
     CDS             <1..570
                     /locus_tag="P5573_04610"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR00524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HRT27374.1"
                     /translation="TGRHAATLLARGDRILTHCFAGPALLYMLRFAREQDMDIKVVCT
                     ETRPYLQGARLTAWSVSQLGVDTTLITDNMAAYTMSRGMVGKVFTAADRIALDGTVAN
                     KVGTLQLAVSARHFHIPFYILAYSGPDRRTPTGEDIPIEMRDPREVLMFRGIPISGKD
                     VKAIYPAFDLTPRELITGIITAQGVEVWE"
     gene            592..1215
                     /locus_tag="P5573_04615"
     CDS             592..1215
                     /locus_tag="P5573_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HRT27375.1"
                     /translation="MKILSIKETFSDQFEELAEKIQEGTNLLLEILDHYEHAEEKIPK
                     LKDIEREADVITHRVYNKLHKTFITPLDREDIYALANKLDSILDLVEEAGAKMSLYKI
                     KGPIPEIKELAQILGKAVFLIRKIIYDMRHHSKNFKTIMDTCVEINTLENEGDQILRK
                     SMVQLFEREDDVIELVKWKEILELVEEATDICEDISNVIEGILLKHG"
     gene            complement(1237..1449)
                     /locus_tag="P5573_04620"
     CDS             complement(1237..1449)
                     /locus_tag="P5573_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27376.1"
                     /translation="MELGDLVLVMDRRHPLLGCVGKVVGKRGEMRPGDPWLLVYVPSR
                     MRSFLIPQSMLKTDEKAAERYEPLYP"
     gene            1599..1901
                     /locus_tag="P5573_04625"
     CDS             1599..1901
                     /locus_tag="P5573_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27377.1"
                     /translation="MEEIKMPIKFHGNYVVIIRTGDAEDRERCQKLTVRRLSEEEKVA
                     SFKNQPPEAVPTHQVTFYDFGCKRIIEGVLKVNTEDRLVIGVKEKDYEFSPFKPRT"
     gene            complement(1924..3252)
                     /locus_tag="P5573_04630"
     CDS             complement(1924..3252)
                     /locus_tag="P5573_04630"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005334.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRT27378.1"
                     /translation="MRFDIAKTGNGMTYEIRNIVGVANRLMEYGKEIYWENIGDPVQK
                     GEKIPEWMKDVLKGILDQDSSYAYSPTKGMEETREFLAERVNKRNGVKIDKEDIIFFN
                     GLGDAIARSYSAIRLDARVIMPEPTYSTHFLAEVLHASFPPNTYKMDPYSNWHPDMRE
                     LEQKVKHRRAIVGILVINPDNPTGFVYPVETLKRIVKIAKENDIFLIFDEIYTNMIYN
                     GKKSAPLSDIIGDIPGMSMKGISKECPWPGARCGWIEFYNSEKDDVFARYVNTILTQK
                     MSEVCSTTLPQMAIPKLLTHPEYEKYLDERVRHYERLSNIAYDILRDVPYIAVNRPNG
                     AFYMSVVFKEGALNNRQTLPIEQPGVRDYIEKILDDKVEQDKRFVYYLLGSTGICVVP
                     LTSFFTSLPGFRMTLLEKDVSKFEHVVKILAEKIVEYVESTSPAVQPMLA"
     gene            3457..4233
                     /locus_tag="P5573_04635"
     CDS             3457..4233
                     /locus_tag="P5573_04635"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF006008.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HRT27379.1"
                     /translation="MAHLYVQFEIKEAVGTITLNHPEKRNALSLAMLKELDGLLDEIK
                     ERQDIKVIIIQGAGKVFSSGHDISEMVGKGMADYREIFEVCSRFMLKLQELPQPVIAK
                     VHGVATAAGCQLVAACDLAVAEEGTQFGTPGVRIGFFCSTPAIPLVRAVGRKRALEML
                     FTGRMVSAREAEQYGLINKAVPADRLDEEVRATAEKIAEASRLTLGIGKQAFYTQVNL
                     SDAQAYAHGNQVMVANLFAEDAAEGLKAFLEKRKPVWKNR"
     gene            complement(4265..>4605)
                     /locus_tag="P5573_04640"
     CDS             complement(4265..>4605)
                     /locus_tag="P5573_04640"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HRT27380.1"
                     /translation="KELLLGLKRDPGFGLVLVCGFGGIYTEVFRDIARRLVPVDRREA
                     EEMLRSLKCYPLLAGVRGETGVDIEGLLDVLERLSFLATRVPDIAELDINPLMADAAG
                     CVAVDARVIL"
BASE COUNT         1103 a         1282 c         1223 g          997 t
ORIGIN      
        1 acgggacgac acgcagcgac gctcctcgcc cggggggaca ggatactcac tcattgcttc
       61 gcaggaccgg ctctccttta catgctccgg tttgcccggg aacaggacat ggatatcaag
      121 gtcgtctgca cggaaacccg tccctacctg cagggtgccc gcctcacggc ctggtccgtg
      181 agtcaactcg gggttgacac cacgctcatc acggacaata tggcggctta taccatgtcc
      241 cgggggatgg tcgggaaggt gttcacggcg gcagaccgga tcgccctgga cggcaccgtg
      301 gccaacaagg tcgggacgct gcaactggcg gtatcagccc gccacttcca tattcccttt
      361 tatatcctcg cttacagcgg accggaccgc cgtaccccaa cgggagaaga catccccatc
      421 gagatgcgcg acccccggga agtgctgatg ttccgcggga tacccatcag cgggaaagac
      481 gtcaaggcta tctacccggc cttcgacctc acgccgcgcg agctcatcac aggcataatc
      541 accgctcagg gcgtcgaagt ctgggaataa acagagagaa agagcagttc cgtgaagata
      601 ctttcgataa aagagacgtt ctccgatcaa ttcgaagagc tggcggaaaa gattcaagag
      661 gggacgaact tactccttga gatactcgac cattatgaac acgctgaaga gaagattccc
      721 aagctgaaag acattgaacg tgaggccgac gtcataaccc atcgggtcta taacaagtta
      781 cacaagacct tcatcacccc cctcgacagg gaggatatct acgctttggc gaacaagctg
      841 gacagcatcc tggacttggt cgaggaggcg ggcgccaaga tgagccttta caaaatcaag
      901 ggccccatcc ccgagatcaa ggagcttgct cagatcttgg gcaaagcggt cttcctcatc
      961 aggaaaatca tctacgacat gcggcatcac agcaagaatt tcaagaccat catggacacc
     1021 tgcgtcgaga taaacaccct cgagaatgaa ggcgaccaga tactgcgaaa gtccatggta
     1081 cagctgttcg aacgggaaga cgacgtgatc gaactcgtca agtggaaaga gattctcgaa
     1141 ctcgtcgaag aggcgaccga catctgcgaa gacatctcga acgtaatcga aggaatcctg
     1201 ttgaaacacg gatagccggc ggtgtcggct ctaccgtcat ggatagagcg gttcgtaccg
     1261 ctccgccgct ttttcgtccg tcttcagcat ggattgagga atcagaaaac tcctcatgcg
     1321 agaaggtacg tagacgagca accaagggtc gcccggtctc atttccccgc gctttccgac
     1381 gaccttcccg acacacccta aaaggggatg ccttcggtcc atgaccaaaa caaggtctcc
     1441 caattccatc tcgttcccct ccccttttat gttctatatg gttttcttta tcatagggta
     1501 ccggcaaata cacggactct tttaccacca ttcttgatgg ataatccgac cgtgatgtgc
     1561 tatgagcaga aaatcaatct ttgccgaaga ggagagagat ggaagagata aagatgccta
     1621 tcaaatttca cggcaactat gtcgtcatca tccggaccgg agacgcggaa gaccgggaac
     1681 gctgtcaaaa attgactgtc cgtcggctct ccgaggaaga aaaggtggca tccttcaaaa
     1741 accagcctcc ggaagcggtg cccacccacc aggtcacttt ttacgatttc ggctgcaagc
     1801 ggatcatcga gggcgtgctc aaggtcaaca ccgaggacag gttggtcatc ggcgtcaagg
     1861 aaaaggatta cgaattttcg ccgttcaaac ccaggaccta aaggttcttg aaaagaaacc
     1921 ggtctacgcc agcatcggct gaacggcggg gctcgtggat tccacgtact ccacaatctt
     1981 ctcggccagg atttttacga catgctcgaa cttagataca tccttctcga gcagggtcat
     2041 cctgaaaccg ggcagggacg tgaagaaaga tgtgagcggc accacgcaaa tccccgtcga
     2101 gccgaggaga tagtacacaa accgcttgtc ctgctcaacc ttatcgtcca ggattttttc
     2161 gatgtaatcc ctcacccctg gctgctctat cggcagggtt tgcctgttgt ttaaagcacc
     2221 ctctttaaat accaccgaca tgtaaaaggc gccattcggc cggttcacgg cgatgtaagg
     2281 aacgtcgcgc aggatgtcgt aggctatatt ggacagcctc tcgtagtgac ggacacgctc
     2341 gtccaggtat ttttcgtact ccggatgagt caacagcttc gggatggcca tctggggcaa
     2401 ggtcgtcgag cacacctccg acatcttctg ggtcaggatt gtgttgacgt acctcgcgaa
     2461 gacatcatcc ttctcgctgt tgtagaactc gatccagccg caccgcgccc ccggccaggg
     2521 gcattctttc gagatgccct tcatgctcat cccggggatg tcgccgatga tgtccgacag
     2581 gggcgccgac ttcttcccgt tgtagatcat gttggtgtaa atctcgtcga aaatcaggaa
     2641 tatgtcgttt tctttggcga tcttgacaat ccgcttgagg gtctctacag ggtatacaaa
     2701 acccgtggga ttatcggggt ttataaccag gatgcccacg atggcgcgcc ggtgcttgac
     2761 cttttgctcg agctctcgca tatcgggatg ccagttactg taaggatcca tcttgtatgt
     2821 gttggggggg aaggaagcat ggagcacctc ggcaaggaag tgagtcgaat aagtcggctc
     2881 cggcatgatc acccgggcat cgagccggat ggcgctgtac gaacgggcga tggcgtcgcc
     2941 gaggccgttg aagaagatga tatcttcctt gtcgatcttt acgccgttcc ttttattgac
     3001 cctctcggcg agaaattccc gcgtctcttc catgcctttt gtcggggaat aggcgtaaga
     3061 gctatcctgg tccaggatgc ctttcaggac atctttcatc cattccggta tcttctcgcc
     3121 tttctggacg ggatccccta tgttctccca gtagatttcc ttgccgtatt ccatcaatct
     3181 attcgctaca cctacgatat tcctgatctc gtaggtcatc ccatttccgg tttttgctat
     3241 gtcaaacctc ataaactgaa gaactcctta agcattacct ctaaacttgg aggttttaat
     3301 acaatcgcaa aaaaaattct acaacttttt atttccgctc gtaagacacc gtttcttatt
     3361 cgatgttttt taggcttgac agtctgtcgg ctttttcttt aatattatca aataatttga
     3421 tattattcga gattacagag aaagaaggag gggaagatgg cacacctgta cgtccagttc
     3481 gaaattaaag aggctgtggg cacgatcact ctcaaccacc cggaaaagag gaacgccctc
     3541 tctcttgcga tgctgaaaga gttggacggc ctgctcgacg aaatcaaaga acgacaagat
     3601 atcaaggtca tcatcatcca gggggcgggc aaggtcttct catcgggcca cgatatttcc
     3661 gagatggtgg ggaaaggcat ggccgattat agagagatat tcgaggtttg cagccgtttc
     3721 atgctgaagc tccaggagct tccacagccg gtgatcgcga aagttcacgg ggtggccact
     3781 gcagccggtt gccagctcgt cgcagcctgc gacctcgccg tcgcggaaga gggaactcaa
     3841 ttcgggactc ccggcgtccg gatcggcttt ttctgctcca cacccgccat accgctggtg
     3901 cgcgccgtag gccggaagcg cgccctggag atgctcttca cggggcggat ggtatccgcc
     3961 cgtgaggccg aacagtacgg gctgataaac aaggcggtcc cggcggaccg actggacgag
     4021 gaagtgcggg cgacggcgga aaagatagca gaggccagtc gcctcactct cggcatcgga
     4081 aagcaggcct tctataccca ggtcaacctc tccgacgccc aggcttacgc ccacggcaat
     4141 caggtgatgg tcgcgaacct cttcgccgaa gacgccgcag aaggccttaa agccttcttg
     4201 gagaagagga agccggtatg gaagaacaga taggacctgt ggggcaaccg aggctgccgt
     4261 aacgtcataa tatgactcgg gcgtccaccg ccacgcaacc ggcggcgtcg gccatcaagg
     4321 ggtttatatc cagttcggcg atatccggca cccgtgtggc caggaaggaa agtctttcca
     4381 ggacatccaa aagcccctcg atgtcgacgc cggtctctcc tcgtaccccg gccaagagcg
     4441 ggtagcactt gagcgaccgt aacatctctt cggcctcccg cctgtcgacc ggcacaagcc
     4501 gacgggcgat gtccctgaag acctccgtgt agataccgcc gaaaccgcag acgagcacaa
     4561 ggccgaagcc ggggtcccgc ttcaacccaa ggagcagctc cttac
//
LOCUS       DAOTMX010000124         4540 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_15602_length_4540_cov_3.299889, whole genome shotgun sequence.
ACCESSION   DAOTMX010000124 DAOTMX010000000
VERSION     DAOTMX010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4540
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15602_length_4540_cov_3.299889"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..1097)
                     /gene="purM"
                     /locus_tag="P5573_04645"
     CDS             complement(54..1097)
                     /gene="purM"
                     /locus_tag="P5573_04645"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HRT27381.1"
                     /translation="MRDKYFYKQAGVDIDNANLFVQKIKPIVRGTFRKEVKGAIGDFA
                     GLFHLDTAKYKMPILVSSTDGVGTKLKIAQMMNRHDTIGVDLVAMSVNDIVVKGAEPL
                     FFLDYIATGKLSLDVGVAIVEGIAKGCSEAGCALIGGETAEMPGMYQDGEYDLAGFCV
                     GIVEADRLIDGSVISMGDRIIGLASSGLHSNGFSLARKIFFEDARLHIDDKVGNLDHS
                     IGHELLKPTKIYVKTILNIFKSFAIKGLVHITGGGFIDNIPRTLPEPCRALITKGSWV
                     VPPIFSIMQKMGNVDETEMFRVFNMGVGMMMIVAEKDAPEILERLTGLGEQAYVIGMI
                     DKRDKDQEPVSFI"
     gene            1344..1727
                     /locus_tag="P5573_04650"
     CDS             1344..1727
                     /locus_tag="P5573_04650"
                     /inference="COORDINATES: protein motif:HMM:NF018753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HRT27382.1"
                     /translation="MTLREVKEILEAEVIVGEDQLEKEVKTAFSADLMSDVLAFATAG
                     SLLLTGVTNPQVVRTAEILDIAAIVMVRGKWPSQETIQLARELRIPILSTRYILFETS
                     GKLHARGISGCIEKVGGKTDAGRTI"
     gene            1708..2142
                     /locus_tag="P5573_04655"
     CDS             1708..2142
                     /locus_tag="P5573_04655"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRT27383.1"
                     /translation="MPEERYDGRFDVEGGNFRNAGQVSSKIKNILKDIGCSSDIVRRA
                     AIVTYEAEINIVSYARKGQIHLLAEPDLVRICVDDEGPGIADIELAMQEGYSTAPAFV
                     REMGFGAGMGLANMKKYSNDFEISSVVDVGTQVKMIINRSEG"
     gene            2151..2492
                     /locus_tag="P5573_04660"
     CDS             2151..2492
                     /locus_tag="P5573_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27384.1"
                     /translation="MAVELEKIVEELNLGVRCCKDRLSRPVSGGYVGDLLSDVMANSR
                     EGDVWITRQIHPNTVAVASLKEHAAVIFVNGSEPTAETVSRAEQEGVPLLVSSMPGFE
                     VAGRIYKMMNP"
     gene            2601..>2816
                     /locus_tag="P5573_04665"
     CDS             2601..>2816
                     /locus_tag="P5573_04665"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRT27385.1"
                     /translation="MLREFRCDLHIHTCLSPCADLEMSPKAIVEKSLKENLDIIAICD
                     HNSSENVVYAMKAAAGKPLVVITGMEVT"
     assembly_gap    2817..2826
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2827..3359
                     /locus_tag="P5573_04670"
     CDS             <2827..3359
                     /locus_tag="P5573_04670"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HRT27386.1"
                     /translation="REEVHLIALFDKLEELEKLQVQVYRRLPGVNDERVFGCQAVVNE
                     LDEVEGFNDHLLIGATTFSIEEAVAAIHALGGLAVASHIDREGFGIISHLGFIPPDLK
                     LDALEVSAAMGIPEARREFAELYRMPFIESSDAHFIRDVGKGVTRMRLGEGSIKELKM
                     AFRKEQGRCIVEGPHA"
     assembly_gap    3432..3441
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3442..3895
                     /locus_tag="P5573_04675"
     CDS             <3442..3895
                     /locus_tag="P5573_04675"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRT27387.1"
                     /translation="DRLIIDINDDGEGMDEETLRKALDPFFTTKKVRRIGLGLPMLAE
                     AAERTGGRLILDSRKGRGTRVRVEFGYSHIDRQPLGDMPGTILALIAGNPEVNFTYCH
                     RRDGLFYRLDTSEIKEEIGDIPVNHVEVLKFIRESMEEGLKGIGFSWH"
     gene            3963..4448
                     /locus_tag="P5573_04680"
     CDS             3963..4448
                     /locus_tag="P5573_04680"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HRT27388.1"
                     /translation="MNDDLLKEFSPEQVEELEKIIEKYKGKPGSLIPVLEEAQVVLDY
                     LPIPVQKKIAKELNLPLAQVYGVVTFYSFFTMTPRGKHTVRVCLGTACYVRGGKKIVE
                     TIEKEYGVKEGETTSDRRFTFETVRCLGACGLGPVMVVDGNVHGKVKPAKLKEILANY
                     N"
BASE COUNT         1131 a         1077 c         1291 g         1021 t
ORIGIN      
        1 gacaagtatg cccacgttga ccattttctc gccccaaatt gctccggatg aaatcagata
       61 aaagatacgg gctcttggtc tttatcccgt ttatcgatca tgccgatgac gtacgcctgc
      121 tcacccaatc cggtcaaacg ttcgaggatt tcgggggcat ctttttccgc gactatcatc
      181 atcatgccga cccccatgtt gaagaccctg aacatttccg tttcatcgac atttcccatc
      241 ttttgcatga tggaaaatat aggagggaca acccaacttc cctttgtgat caaagcccgg
      301 cacggctccg gtaatgtcct cgggatgttg tcgatgaaac cgccgcccgt gatatggacc
      361 aggcccttta tcgcaaaact tttgaaaata ttcaatattg ttttcacata aatctttgtc
      421 ggtttgagaa gttcatggcc gatggaatgg tcgaggttgc cgactttgtc gtcgatatgc
      481 agccgggcat cctcgaagaa tatctttctg gccagggaga agccgttact gtgaaggccg
      541 ctcgatgcca agccgatgat cctatctccc atgctgatga cagaaccgtc tatcagacgg
      601 tctgcttcga cgatcccgac gcaaaatccg gcaaggtcat actcaccatc ctgatacatc
      661 ccgggcatct cggctgtttc gcccccgatc aatgcgcacc cggcctcgct gcagcctttt
      721 gctatgcctt ctacgatggc aacgccgacg tcaagactca acttgccggt cgcaatatag
      781 tcaagaaaaa aaagaggctc cgcccctttg acaacgatgt cgttgaccga catcgcgacc
      841 agatccacgc ctatcgtgtc atgcctgttc atcatctggg cgatcttcaa ctttgtaccg
      901 acgccgtccg tagaagatac caggatcggc atcttatatt ttgccgtgtc cagatgaaaa
      961 agtcccgcaa aatcgccgat agcccctttg acttccttac ggaaagtacc ccgcacgatc
     1021 ggctttattt tctggacgaa aagattggcg ttatctatat cgacgccggc ctgcttgtaa
     1081 aagtatttat ccctcatgca tgtcctgtac gtagatttcc aaggtgactg caacgttgta
     1141 ggggaatata gagcaacacc ggggaattgt caatcatcca catgccttgg cgcggtggat
     1201 ttaggttgac ataagaattt gcatatgtta cggaaaatcg catttacgtt aaggaccgct
     1261 gagcggggta ataaggccgc agcaatcaaa tcggaccctt ttatgggtca agaccgtaag
     1321 tactttgtgc aaaggaggtc gaagtgaccc ttcgcgaagt aaaagagatt cttgaagctg
     1381 aggtaatcgt aggtgaggat cagctggaaa aagaagtgaa gaccgccttc agcgccgatt
     1441 tgatgagcga tgttctcgcc ttcgcgaccg caggaagcct tcttttgacg ggagtgacga
     1501 acccgcaggt cgtccgcacg gccgagatcc tcgacatagc ggcgatcgtg atggtcaggg
     1561 ggaaatggcc ttcccaggaa acgatccaac ttgcccggga gttgaggata ccgattctct
     1621 cgacgagata cattcttttc gagacctccg gcaaactgca cgccagaggg attagcggct
     1681 gcatcgagaa ggttggtgga aagaccgatg ccggaagaac gatatgacgg tagatttgac
     1741 gtcgagggcg gtaatttccg gaatgccggt caggtctcaa gtaagataaa gaatatcctc
     1801 aaagacatcg gctgcagcag tgatattgtc aggagggccg ccatcgttac ctacgaggcg
     1861 gaaatcaaca tcgtctctta cgctagaaaa ggtcagatcc atcttcttgc ggaaccggat
     1921 cttgtaagaa tctgtgttga tgacgagggt ccgggcattg cagacatcga actcgccatg
     1981 caggaagggt attcgacggc cccggcgttc gtaagggaga tgggtttcgg tgccggaatg
     2041 gggctcgcca acatgaagaa atattcgaat gacttcgaaa tatcctccgt agtcgacgtc
     2101 gggacgcagg tcaagatgat catcaatcgg tcggaaggat agccggacag atggccgtcg
     2161 aactggaaaa aatcgtggag gaattgaatc tgggagtccg ttgctgcaag gacaggctgt
     2221 cgaggccggt ttccggcggc tatgtgggag accttttgag cgacgtcatg gcaaacagcc
     2281 gcgagggtga cgtctggata acaaggcaga tccacccaaa caccgtagcg gtcgcaagcc
     2341 tgaaagagca tgcggcggta atttttgtca acggaagtga acccacagcc gagacggtat
     2401 ccagggctga acaggagggg gttcccttgt tggtgtcgag tatgccgggc tttgaagtgg
     2461 ccggcaggat atacaagatg atgaatccgt aaatttgcat ggactaagga agcaggtctg
     2521 ctcattcaca cggcgatgaa tctcggtcag gataagtcag ctcgattggt tggcggatcg
     2581 gtgtttcagg aagcaatgtc atgctcaggg agtttcgttg cgacctccat attcatacat
     2641 gcctttcgcc ttgtgccgac ctggagatgt ctcccaaggc aatagttgaa aaatcactga
     2701 aagagaacct ggacatcatc gcaatctgtg accacaactc ttcggaaaac gtggtctatg
     2761 caatgaaggc agcagcgggg aagccgctcg tcgtcatcac agggatggaa gtgacannnn
     2821 nnnnnncgcg ggaggaggtt cacttgattg cgctctttga taagcttgaa gagctggaaa
     2881 aacttcaggt tcaggtgtac cgtcgtctgc cgggcgtgaa cgacgagagg gttttcggat
     2941 gtcaggccgt cgtcaatgaa ctcgatgagg ttgaaggttt taacgatcat ctgctcatcg
     3001 gtgcgacgac tttttcgatt gaagaagccg ttgccgccat ccatgctctg ggcgggcttg
     3061 cggtggcttc tcatatcgac cgggagggat tcgggatcat cagccatctg ggattcatac
     3121 ccccggacct gaagctcgac gcactggagg tttcggcggc gatggggatt ccggaagccc
     3181 gccgggaatt cgccgagcta taccggatgc cctttattga gtcgtcggac gcccacttca
     3241 tccgggatgt cggtaaagga gtaacccgga tgcgccttgg cgagggtagt atcaaggaat
     3301 tgaagatggc cttccgaaag gagcaagggc gctgcatcgt ggaaggaccc catgcttgag
     3361 ctttccctcc acatcctcga catcgtggag aactcgacga gggccggcgc ccgaaatgtc
     3421 caaatcgata tnnnnnnnnn nggacaggct catcatcgat atcaatgatg acggtgaggg
     3481 gatggacgaa gagacattgc gaaaagccct tgatccattt tttacgacga agaaagtcag
     3541 gcggatcggg ctgggattgc cgatgctcgc ggaggccgcc gaaaggaccg gggggcggct
     3601 catccttgat tcgcggaagg gaaggggaac cagggtccgc gtcgaattcg ggtacagtca
     3661 tatagacagg caaccactgg gggatatgcc gggaacgatt ctggcgttga tagccggcaa
     3721 cccggaggtt aacttcacct attgccacag gcgggacggc cttttttaca ggttggatac
     3781 ctccgagata aaggaagaga tcggggatat tccggtcaat cacgtggagg ttttgaagtt
     3841 catcagggaa tcgatggaag aagggctaaa gggtatcgga ttttcgtggc attgacatca
     3901 aaaagatgcc gggccatttc taactcagcg gtattactaa aaacggcggg aggagcagat
     3961 ccatgaatga tgacctgttg aaagagttca gccccgaaca ggttgaagag cttgagaaga
     4021 tcattgaaaa gtataagggt aagccggggt ccttgattcc ggtcctggaa gaagcccagg
     4081 ttgttcttga ttatcttccg ataccggttc aaaaaaagat tgccaaggag ttgaacctgc
     4141 cgttggctca agtttacggc gtggtcactt tttattcctt cttcaccatg acccccaggg
     4201 ggaagcacac cgttcgtgtc tgtctcggga cggcctgtta cgtccggggt gggaagaaga
     4261 tcgtggaaac catcgagaag gagtacgggg tgaaagaagg ggagacgacc tcggacagga
     4321 ggttcacctt tgaaaccgtg cggtgtctcg gtgcttgcgg gctggggccg gtcatggttg
     4381 tggacggcaa cgtccacggc aaggtcaagc cggcaaagct aaaagagatt cttgccaatt
     4441 ataattagca ggggggccat catggcgaaa atcaagatag aagacctgaa gaagattaaa
     4501 gaaaaggtac aagccgagac ctcgctaagg gaaggccagc
//
LOCUS       DAOTMX010000125         4510 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_15726_length_4510_cov_2.842873, whole genome shotgun sequence.
ACCESSION   DAOTMX010000125 DAOTMX010000000
VERSION     DAOTMX010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4510
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15726_length_4510_cov_2.842873"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="P5573_04685"
     CDS             complement(<1..863)
                     /locus_tag="P5573_04685"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRT27389.1"
                     /translation="MDIRAHGKDFDRFVNRAKNEYGIRYIRSIPSTIKELQQTKNLLV
                     KYVQEDGTLVEEEFEMVVLSVGLTPPHEARELATALGIGLEDHGFCATDLENPVKTTR
                     DGVFVCGAFGGPKDIPETVMEASGAAACASSIIAERRGTMIVEEELPMEKDLRGIGPR
                     IGVFVCHCGINIGGVVNVPEVRDYAKTLPNVVYVADNLFTCSQDTAVKMAEVIEEYKL
                     TRVVVASCSPRTHEPLFQENCEKAGLNPYLFEMANIRDQDSWVHMNEPEKATEKAKDL
                     VRMAVAKAQFL"
     gene            complement(1572..3122)
                     /locus_tag="P5573_04690"
                     /pseudo
     CDS             complement(1572..3122)
                     /locus_tag="P5573_04690"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            3633..4469
                     /locus_tag="P5573_04695"
     CDS             3633..4469
                     /locus_tag="P5573_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HRT27390.1"
                     /translation="MFKITEKTFLQENVVRMVLEAPEIARKRKAGQFVVLMIDEKGER
                     IPLTIVDSDNENGTLTIIFQVVGKTTAYLAELNPGDSILHVLGPLGHPTEVENFGTSV
                     CVGGGVGVGVVYPIAAALKKAGNKVISIIGARTRDLLILEDEMGRVSDRLLVATDDGS
                     YGFHGFVSAVLQSVIDLGEKIDRVFAIGPVPMMRVICDVTRPYGIKTIVSLNPIMVDA
                     TGMCGACRVSVGGTTKFGCVDGPEFDGHQVDFGLLMSRLRMYCDQERQSMDLFRCGRH
                     GK"
BASE COUNT          900 a         1317 c         1252 g         1041 t
ORIGIN      
        1 tgcaggaact gggccttggc caccgccatc cgtaccaggt cctttgcctt ttccgtcgcc
       61 ttttccggct cgttcatgtg aacccatgaa tcctggtcgc ggatgtttgc catctcgaac
      121 aggtagggat taagtccagc cttctcgcag ttttcctgga atagcggttc atgggtccgg
      181 ggagaacacg aagcgacgac tacccgcgtg agcttgtact cttcgatcac ctcggccatc
      241 ttcaccgccg tgtcctgtga gcaggtgaag agattatcgg ccacgtacac gacattcggt
      301 aatgtcttgg cgtaatcccg cacttccggg acattgacca cacccccgat gttgatcccg
      361 caatgacaga caaaaacgcc gatccgggga cctatccctc gcaggtcttt ctccatgggc
      421 agttcttctt ctacgatcat cgtgccccgg cgttcggcga tgatactcga ggcacaggcg
      481 gcggcgccgc tcgcctccat gaccgtctcg gggatgtctt ttggaccgcc gaaagcaccg
      541 cagacaaaca ccccatcccg tgtcgtcttt accggattct cgaggtcggt ggcacagaaa
      601 ccgtgatcct cgagcccgat ccccagggcc gtcgccaatt cccgcgcctc gtggggtggg
      661 gtgagaccga ccgacagcac caccatttcg aattcttctt ctaccagcgt accgtcctcc
      721 tgcacgtact tgacgagcag gttcttcgtc tgctgcaact ccttgatagt ggaggggatg
      781 gaacggatgt agcgtattcc gtactcgttc ttcgcccgat tgacgaaccg gtcgaagtcc
      841 ttaccgtgcg cccggatatc catgaagaag atagtcggtt ccagctcttt gacgtgttct
      901 ttcccgataa tcgcttcctt agtggcgtac atacagcata cggaagaaca ccagctgttt
      961 ccgcaggccg tgtccctcga acctacgcat tggatgaagg cgattttctt cggttccttc
     1021 ccgtcggaca atctttggac gtgcccgaaa tagggaccgg aggcggagag gatgcgctcg
     1081 aactggatac tcgtgacgac gttggcgtat acaccgtacc cgtactccga ccggagggct
     1141 gcatcgaagg tctcgaaccc cggtgcggcg atgatggctc ctacctcgat cgtttcttct
     1201 tcgaaggtat cttcatgatt gatcgcctgg gcgaggcagg cctcaacgca ctggtaacat
     1261 tccgagcaaa taccgcaact caggcagcgt tgcgcctccc gcaccgcctc ttcttcgctg
     1321 aagccgatcc ccacttcgcg gaaatcgact tttcgctctt ccggcttgac atgggggtac
     1381 ttcacccggc tgcttttggg aacacgggag gtatcgccgg ggtcggcaag tccctgcgtc
     1441 caatcccgct cccggcccgc cttgatgtct tccccgcgga gatagcgatc gatagacctg
     1501 gcggcctctt ttcccgcgta tacggcttta actacggtct gggggccggt caccacgtct
     1561 ccaccggaaa acaccccagg gccatcggta gcgaaggtta cggggtccac atcgatattg
     1621 ttccatttgt tgacggaaac cccgcttccc tcggtgagga aggacaaatc cgcttcctgc
     1681 ccgatggcgg ggatcacggc gtcgcatggg acgatgaatt ccgagccctt gatttcgatg
     1741 gggcgcctcc gcccgcttgc gtcaggttct cccagttcca tgcgggtaca ctctataccc
     1801 gtgaccttgc cgttctcacc gatgatgcgt ttcggcgcga cgaggaattc catccgcact
     1861 ccttcttcca aggcttcctc gatttcttcg ggcgacgccg gcatttcggc gcgggaccta
     1921 cggtacagga tgaagacatc cttcgacccc gtcctgatcg ccgtgcggac ggcgtccatg
     1981 gccacgttgc cgccgccgat cacggctact caatcgccga gatacacctt ctcccccagg
     2041 tttatccgtt ggagaaagtc tacgccgtgg accacgcctt gcaggtcttc ccccggcaca
     2101 ttcagtttgc tgctgcgcat cgtcccgcac ccgatgaaca cggcatcgta atctttcttt
     2161 agctgatcaa aggaaatatc cttcccgata cgggtgttga gatggatctt tacgcccagg
     2221 tcctcgatca cctttatctc tgcccgcagt acgtctttcg gcaagcggta ctcggggatt
     2281 cccacggcca accacccacc ggcgacgggc agggcttcga agacgtccac ttgataaccc
     2341 tcgatggcga ggtaataggc gcacgtcagc ccggcgggac ccgcccccac gacagctacc
     2401 ttcttcccat tgctttcctt cttctcgggg aggtagcggg tttcatcctt caagtcaagg
     2461 tcggccacga acctcttcag atgcattatg tctatcggct cgtcgacatc acccctcttg
     2521 cacaccgact cacagggatg gttgcagacc cggccgcata cgatcgggaa cgggttgtcc
     2581 tctttgatga gttttaaggc ctccttaaac ttacccgcgg cgatcagcgc cacgtatccc
     2641 tggacgctga tatgggtcgg acaggcggcc ttacacggcg acgtaccccg tttgtcgatg
     2701 gcgaagccgc tggggatggc ctgcggaaag tgacgataga tggcctgccg ttccgacagt
     2761 ccttcgttga agtccgacgg gatgtttatc gggcatacct tcgcacaatc gccgcacccc
     2821 gtgcacttgt ccagatccac ataacggggg gcccgcgtca tcttcacccg gaaatgaccg
     2881 gcttctcctt ccacggccgt tatcgtccgc cgggtgagga tttctacatt cggatggcgg
     2941 ccgacttcta ccagcttggg ggatagtata caggcagagc agtcatttgt gggaaaggtc
     3001 ttgtcgagct gagccataac cccgccgatg ctgatgccct tttctacgag gtacaccttc
     3061 atgcccgaat tggcgagatc caatgacgcc tggatgccgg cgatgccggc ccccgctacc
     3121 atcactgccc cgattttttc cttatctttt gtcacgtgtt gccacctcgc gatgtgttta
     3181 ccacccttct cggaagggcc atgttagacc tactgaaaca tcgcccctga gaaagaccgg
     3241 gcgagtcctg cgagaatcac ctgaatagac tggcaggcgg tatcacgatt gggttggctc
     3301 tgtcaagggc aaaatgcctc tcatgtctgt ttgttcttgg tgaacgacaa attttgtctt
     3361 ggcatttatg atgcgttttg ctatagttac acctatttgc aacatactga gatcactgag
     3421 tatcaggccg acgggaggac gaatataagc tgttgcctat gccgcgacat gtgctggcgc
     3481 gtaatctcga aagtaccccg ggcatcatca aaaaggtcgc ctcctttcgt ggcaaccttg
     3541 cgaatgcctt ggtagtacgg ttaaggacgg ccgtctatca tcacaagaag catattccat
     3601 taccttaacc ggtttcgaca gaggggaaga cgttgttcaa aatcactgaa aagacatttt
     3661 tacaggaaaa cgtggtccga atggtcttgg aggccccgga gattgctcgt aagcgaaagg
     3721 ccggccaatt cgttgttctg atgatcgacg aaaaagggga gaggatcccg cttaccatcg
     3781 tcgattccga caacgagaat gggacgctga ccatcatctt tcaggtcgtg ggcaagacga
     3841 cggcctacct tgcggagctc aatccggggg attctattct gcatgtgttg ggtcccctgg
     3901 gccatcctac ggaggtcgaa aacttcggaa cctccgtctg tgtgggtggc ggcgttgggg
     3961 tcggcgtcgt gtatcccata gccgcggcgc tcaagaaggc gggcaacaaa gttatctcga
     4021 tcatcggggc ccgaacgaga gacctcctta tcctcgaaga tgagatggga cgggtgagcg
     4081 accggctgct ggtggcaacc gatgacggca gttacggttt tcacggtttc gtgagcgcgg
     4141 tgcttcagag cgtcatcgat ttgggggaga agatcgacag ggtctttgcc atcggaccgg
     4201 ttccgatgat gcgggtgatt tgcgatgtta cgaggccgta tggcatcaag acgatcgtca
     4261 gcctgaaccc gatcatggtg gatgccacgg ggatgtgcgg cgcctgcagg gtgtccgtag
     4321 ggggaacgac taagttcggc tgcgtggatg gcccggaatt cgacggccat caggtggact
     4381 tcggcctctt gatgagcagg ctcaggatgt actgcgacca ggagaggcaa tctatggatt
     4441 tgttcaggtg cggtcggcat ggaaaataga gaacaggttg aaagaaaaga aaaaaaggag
     4501 aaggtcccca
//
LOCUS       DAOTMX010000126         4501 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_15769_length_4501_cov_3.645524, whole genome shotgun sequence.
ACCESSION   DAOTMX010000126 DAOTMX010000000
VERSION     DAOTMX010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4501
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15769_length_4501_cov_3.645524"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..230
                     /locus_tag="P5573_04700"
     tRNA            137..230
                     /locus_tag="P5573_04700"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:171..173,aa:Ser,seq:gga)
     gene            711..1172
                     /locus_tag="P5573_04705"
     CDS             711..1172
                     /locus_tag="P5573_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27391.1"
                     /translation="MGETTEWLARTGINVISIELSSDLHRLATERLGRYRNIMLLQGD
                     SGQLLPELIASFTEPVCFWLDAHFSGGETAKGVTDTPILQELEAILEHPIRGHVILVD
                     DARCFTGEDGYPFLDEVLVLVRKSGFYRAEVSTDIIRITPIKEWMSQPRNY"
     gene            1272..1370
                     /gene="ffs"
                     /locus_tag="P5573_04710"
     ncRNA           1272..1370
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="P5573_04710"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            1521..3176
                     /gene="dnaX"
                     /locus_tag="P5573_04715"
     CDS             1521..3176
                     /gene="dnaX"
                     /locus_tag="P5573_04715"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HRT27392.1"
                     /translation="MDYLVLARKWRPQVFEDVVGQDHVVKTLKNAIRRDRVAHALIFS
                     GPRGIGKTSVARILAKALNCEKGPAETPCNECTNCKEITEGVSMDVREIDGASNRGID
                     EVRELRENVKFLPLTSRYKVYIIDEVHMLTKEAFNALLKTIEEPPPHVIFMFATTEVH
                     RVPATILSRCQRHEFRRMSIKQISATLRKIATAEGIEVSDAGLSLIAEGAEGSLRDAE
                     SIFDQVISYAGTVVKDGDVETLLGFTDRKLLYDISRAVIGRDARAALGILDEGYYAGL
                     DMKQFYQMLQRHFMNLLLVKVADGAGRFIDLPDFEVTELKKQVGSASGETLKRLLDIL
                     MAEDEEVRKSLDPKLNLEFILVKMASLEPLIPVHELLDRVEGMERRLTGKAQPLKASP
                     IAPKEPEREKAAPTEMPVVEPTAGDLWEGFKETVKKRSNPLWSMIEPGSLVGYEKGYL
                     RIGFPKGYVFLDHLNDPFQKDRLAAIGRDFFGEEVTVKIEAGVGDGSITAPGNGLPAN
                     GGNNDIKKEALNHPMVQKVMDIFEGAEIKEIITRKQTGDPLRG"
     gene            3255..3566
                     /locus_tag="P5573_04720"
     CDS             3255..3566
                     /locus_tag="P5573_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HRT27393.1"
                     /translation="MANFQNIIKQAQQMQAKMVKLQEELASRTVEASAGGGMVTAVVN
                     GKHELVSLKVEKDVVNPDDVEMLQDLIVAAVNEAVRKAQEMAQAEMAKLTGGLQIPGL
                     T"
     gene            3587..4189
                     /gene="recR"
                     /locus_tag="P5573_04725"
     CDS             3587..4189
                     /gene="recR"
                     /locus_tag="P5573_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HRT27394.1"
                     /translation="MMTGYPAPMRRLIKELSRFPGVGEKTASRLAAFILHTPREEARR
                     LAESIMEVKEKIRLCATCFNLAEGEFCDVCKDTTREKEVICVVEEPDSLIAIEESGSF
                     RGTYHVLHGALSPLDGVGPERLRLQELRDRIELNGVREVIVATNPSVQGESTALLITR
                     MLKEKDVRVTRIALGVPVGGDLKYVDKMTLAKAIEFRRGM"
     gene            4198..>4501
                     /locus_tag="P5573_04730"
     CDS             4198..>4501
                     /locus_tag="P5573_04730"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF017493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HRT27395.1"
                     /translation="MEPIREVSCETVTETVRRLFIEANTELGEDILEAVNLACAAEEL
                     DIARLALEQICENARIAREELVALCQDTGLAVVFAEVGQDVHIVGGSFGASVQEGVR"
BASE COUNT         1082 a         1124 c         1395 g          900 t
ORIGIN      
        1 gatttggggg ccaaaagctc tcacgggggt tcgaatcccc ctctctccgc cagaatatca
       61 gactttcgat gtcggacggc gggaggcagg gaacggcgat aaagtgtcgc tgtgactcga
      121 tgtccaaaac ctgagtggag agatgcccga gaggccgaag gggcacgact ggaaatcgtg
      181 tgtacgccca caaaggtgta ccgcgggttc gaatcccgct ctctccgcca taagtcaggt
      241 gtcaggcatt ggacggtggg caggaggtaa ggcgcaggtc tttatacgat gaggcaaaca
      301 ttatgtttgc gtctaaagga gggcttacag gcttaggcgt ttctatcttc agagaagagc
      361 ctaagagggg ctgtgagggg tgatacttaa cttgtgtgat acatgggcaa gtttagtcaa
      421 agaagcggcg attgcccttt tttgtcgtta gcgtacaaag gcgggatacg tgttgagctt
      481 gaggcaagag ggagcaggta gctttaagaa tgacggcatc acccaagaca gcgcattgca
      541 ataaacccgg gccttcacat tttattgaac acattcgccg attacgggct ctctcaaagt
      601 gggtgctgtc gggatgtcca tcaccccccc cacacattgt gaaattatat gtgatttggg
      661 catacttaag agagctaagg ttagagacgg ttgttgagac gggcacgtat ttgggggaaa
      721 ccacggaatg gttggcccgc accggtatca acgttatcag tatcgagctt tccagtgacc
      781 tccaccgcct cgcgacagag cggctcgggc gctaccgtaa catcatgttg ttgcaaggcg
      841 acagtggtca gttgcttccg gaattgatcg caagtttcac cgaacctgtt tgtttttggc
      901 tcgatgcaca cttcagcggg ggcgaaaccg ccaaaggagt aacagataca cccatcctcc
      961 aggaactgga ggcgatcctg gaacatccta ttcggggcca cgtcattctg gtcgacgacg
     1021 cgcgttgttt tacgggtgag gacggatacc cgttcctgga tgaagttctg gttttggtgc
     1081 ggaagagcgg cttctaccgg gccgaagtct caacggacat tattcgtata acaccgatta
     1141 aagagtggat gtctcaaccg cgtaattact gaaggactgt actggtcggt cgtacaaata
     1201 agcgaggtct ttagcattca ttcgttcaaa aaagagaatc ttttgatgcc cggtgcctga
     1261 agaaaaatga tagccgggtc ccgtgcaacg taggcgtgcg aaccccgtca ggtccggaag
     1321 gaagcagcgg tagcaatgac ctccgggtgc tgcgggtaaa cctggctatc atctttttga
     1381 cccggtagaa gccgggcagt agttcaaggg tcgaagttaa gacacggacg caccccttca
     1441 ctcctttccc tcattactgc agtgcctgtc ttatcttttg aattgaagcc ttaagctggc
     1501 aaccctcagg ggggagtttt gtggattatc tcgttttggc ccgaaaatgg cgaccgcagg
     1561 tttttgagga tgtcgtcggg caggaccacg tcgtcaagac gctgaagaac gccatccgcc
     1621 gcgaccgtgt cgcccatgcc ctgatcttca gcggaccacg gggaataggg aagacttcgg
     1681 tagcccgtat ccttgccaag gcccttaact gcgagaaagg ccccgccgaa acaccctgta
     1741 atgagtgcac gaactgtaag gagatcacag aaggggtctc catggacgtg cgggagatcg
     1801 acggcgcctc gaaccgcggc atcgacgaag tgcgggagtt gagggaaaac gtcaaattct
     1861 tgccgctaac ctcgcggtac aaggtttaca taatcgatga ggtccacatg ttgaccaagg
     1921 aggccttcaa tgccctcctg aagacgatcg aggaacctcc gccccatgtc atcttcatgt
     1981 ttgcgacgac ggaggtccac cgggttccgg ccacgatcct ctcccggtgc cagcgccacg
     2041 agtttcgccg gatgtctatc aagcagatat ccgctacgct caggaagatc gcgacggctg
     2101 aggggatcga ggtgagcgac gccggccttt ccctgatagc cgaaggggcg gaaggtagcc
     2161 tgagggatgc ggagagcatc ttcgaccagg tcatctctta tgcagggacc gtggtcaagg
     2221 acggcgacgt cgaaacgctc ttgggtttta cggacaggaa gctcctctat gacatttccc
     2281 gggccgtcat cggtcgggac gcaagagccg ccctgggtat tctcgatgaa ggatactatg
     2341 cgggcctcga catgaagcaa ttttaccaaa tgttgcagag gcacttcatg aacctgcttc
     2401 tggtgaaagt tgccgatggc gccggacggt tcatcgacct gcccgatttt gaggtaacgg
     2461 agctgaaaaa acaggtcggc agcgcgtcgg gagaaacgct gaagcgactg ctcgacatcc
     2521 tgatggcgga agatgaagag gtacgaaaga gcctggaccc gaaactgaac ctcgagttta
     2581 tcctcgtaaa aatggcgtcg ctcgaaccgt tgataccggt gcacgaattg ctggatagag
     2641 tggagggtat ggaaaggcgg ttgacgggta aggcccagcc cctcaaggca tcaccgattg
     2701 cccccaaaga acctgaaagg gaaaaggcag cgccgacgga gatgccggtc gttgaaccga
     2761 cagcgggtga cctttgggaa ggcttcaagg agacggtgaa gaaaaggagc aacccgctgt
     2821 ggtccatgat agaaccggga agcttggtcg gctatgaaaa gggttatctg aggataggtt
     2881 tccctaaggg gtacgttttc ctggaccatc tgaatgaccc cttccagaaa gatcgcctgg
     2941 cggcgattgg gcgggacttt ttcggcgaag aggtgactgt caagatagaa gcgggcgttg
     3001 gagacggctc tataacggcc ccgggaaacg gccttccggc taacggcggt aacaatgaca
     3061 tcaagaaaga ggcgctgaac cacccgatgg tccaaaaggt catggatata ttcgagggag
     3121 cggagatcaa ggagattatc acgagaaaac aaaccggtga ccctttgcga gggtgatccg
     3181 ggcaagcctg gatgggaggg gtgtaaattc cgggtaggcg gagagccttt tcatccaagg
     3241 ggggaggaaa agcattggcg aattttcaaa acatcataaa acaggcccag cagatgcagg
     3301 ccaagatggt gaagctccag gaggaactcg cttcgaggac cgtggaggcg tcggccggcg
     3361 ggggtatggt gacggccgtc gtgaacggca agcacgagct cgtgtcgttg aaggttgaaa
     3421 aagacgtcgt caaccccgat gacgtggaaa tgctccagga cctgatcgtg gcggcggtga
     3481 acgaggccgt ccgcaaggcg caggaaatgg cccaggcgga gatggccaaa ctcaccggcg
     3541 gtctccagat acccggcctt acctgaagca ttcggaagag atgatcatga tgaccggtta
     3601 ccccgcaccc atgcgccgtt tgataaagga gctgagccgt tttcccggcg tcggcgaaaa
     3661 gacggcctcg cggctcgcgg ccttcatcct ccatacaccc agggaagaag cgcggaggct
     3721 tgcggagagc atcatggagg taaaggagaa gatccgtcta tgcgcaacct gtttcaactt
     3781 ggcggagggc gaattctgcg atgtatgtaa ggacacgaca cgggagaagg aagtaatctg
     3841 cgtcgtcgaa gaacctgatt ccctcatcgc catcgaagaa agcggcagtt tccggggaac
     3901 ctaccatgtc ctccacggcg ccctgtctcc gctagacggg gtcggcccgg agcggctgcg
     3961 gcttcaggaa ctccgggaca ggatagagct caacggggtg agagaagtaa tcgttgccac
     4021 caatcccagc gttcagggcg aatccacggc gcttctcatc acgaggatgc tgaaggagaa
     4081 agacgtgagg gtgacgagaa tcgcgctcgg cgtcccggtg ggcggcgacc ttaagtacgt
     4141 ggacaagatg acgctcgcca aggcaatcga attccgccgg gggatgtgac ccgcctcatg
     4201 gaacccataa gggaagtatc ctgcgaaacc gtgacagaga ccgtcagaag gctgttcatc
     4261 gaagccaaca ccgagcttgg agaagacatt ctggaggccg tcaacctggc ctgtgcagcc
     4321 gaggaattgg atatagcccg cctcgccctt gagcagatat gcgaaaatgc ccggatagcg
     4381 agggaagagc tcgtggccct atgtcaggat acaggcctcg ccgtggtctt cgccgaggtc
     4441 ggtcaggacg tccatatcgt gggtggctcc ttcggggcat cggttcagga gggggttcgg
     4501 a
//
LOCUS       DAOTMX010000127         4452 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_15964_length_4452_cov_3.640437, whole genome shotgun sequence.
ACCESSION   DAOTMX010000127 DAOTMX010000000
VERSION     DAOTMX010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4452
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15964_length_4452_cov_3.640437"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="P5573_04735"
     CDS             complement(<1..401)
                     /locus_tag="P5573_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27396.1"
                     /translation="MQEKILKRVQVNMPFHSLYRKYLPMVVEKGINPEICFSHDDIEN
                     FMVQDYVRVAEVLIDHGLTVTFHAPFMDLRPGAVDQKIRQASIERLMQVFDIAPFFRP
                     NCIVCHPSFDDRYYVSTEEAWVENSLDTWGK"
     gene            583..963
                     /gene="rsfS"
                     /locus_tag="P5573_04740"
     CDS             583..963
                     /gene="rsfS"
                     /locus_tag="P5573_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HRT27397.1"
                     /translation="MSTRKAVLLIVNTILKKKATNVVVLKVKKLSSIADYLVICSGSS
                     DRQVQAIASSIEEDMKKETKLPLGVEGDRIGKWILIDYGDVVIHVFLEPVREFYDLER
                     LWSDVPRMEIRDDVTEITSLDKGM"
     gene            977..1684
                     /locus_tag="P5573_04745"
     CDS             977..1684
                     /locus_tag="P5573_04745"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HRT27398.1"
                     /translation="MTGLADLIFPPACCSCGALLGRGMTTFCGACLSEIRFIRAPLCP
                     VCGIPYPNEEEINHRCADCIRSKESFSLARAVGVYDAVLLEVIHRFKYRGRINLGEVL
                     GRFMAEFRYEDLRIEDYSLIVPVPLHRRRLKERGFNQAVVLAREISRRHAVPMDFGVL
                     KRKIYTEPQTGLGRDDRLRNIRGAFEVVRPEAVKEKKVLLIDDVYTTGSTVRECARVL
                     GKCGASEVAALTLARAV"
     gene            1728..2210
                     /gene="rfaE2"
                     /locus_tag="P5573_04750"
     CDS             1728..2210
                     /gene="rfaE2"
                     /locus_tag="P5573_04750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HRT27399.1"
                     /translation="MVKEKIRSWEELKGEVDRLKARGKKIVFTNGCFDILHVGHTRYL
                     GEAKKLGDVLVVAVNSDSSVKAIKGEKRPLVPETERVEVVASLEAVDFVTIFAEETPV
                     TLIEELKPNVIVKGGDWDEKNVAGRESVRKWGGRVAIIPQVEGVSTTNVVEKIRKIYA
                     "
     gene            2311..2673
                     /gene="dksA"
                     /locus_tag="P5573_04755"
     CDS             2311..2673
                     /gene="dksA"
                     /locus_tag="P5573_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HRT27400.1"
                     /translation="MKPEKLEYFRGLLNQKINELLGGAEKTVSEMLDEKENFPDPNDR
                     ASLESDRNFELRIRDRERKLIKKMQEALERIDDGTFGICERCGGPISEKRLMARPVTT
                     LCIECKTKEEKIEKQRGE"
     gene            2859..>4452
                     /gene="priA"
                     /locus_tag="P5573_04760"
     CDS             2859..>4452
                     /gene="priA"
                     /locus_tag="P5573_04760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HRT27401.1"
                     /translation="MYVEVAVAVPSEKTFTYAVPENFAGDIALGKRVLVPFGRKRLTG
                     YIVGILGQAEREGLKEILEVLDETSLFKEDDYEFYRWAAAYYMYPLGKALEEILPGGI
                     NIDSGRWVCLVGDGGADGLTERERTILDILRKNPGGLSVASLQREADSRQLLRSLNRL
                     QQKGMIRIEERMARPAVTARREKFVSMAPGWCNGRLSEKAGAVVQYLSEAGETTFSEL
                     RRRFGVTADTVRRLGEKGAVVVTEREVYRKASAGPTIAKMDAVTLNSAQEGAAKEILK
                     GLLSGKFSPFLLHGVTGSGKTEVYFRALAEALDLGGSVIYLVPEIALTPQLLSRLRDR
                     FKEHEMAVLHSGVPAAVRYDQWRMVARGDIRLVVGARSALFAPVRDLRLIIVDEEHDN
                     SYKQDERMPYNARDLAVVRARQRGATVILGSATPSIQSYSNALRGKYRHLVLTDRPKG
                     LGLPEVEVLDLRREREEKGKTPLLSRPLVEAIGETLETGKQTLLFLNRRGFHTFLFCA
                     DCGYVLKCLNCSVSLTFHAASGI"
BASE COUNT         1068 a         1081 c         1358 g          945 t
ORIGIN      
        1 aacttgcccc acgtgtccag gctgttttcg acccaggcct cttccgtcga cacgtagtaa
       61 cgatcgtcga aagagggatg acagacgatg caattcggcc tgaagaacgg cgcgatatcg
      121 aagacctgca tgagcctttc gatggaggcc tgtctgattt tttggtccac ggcgcccggg
      181 cgcaggtcca tgaaaggcgc gtggaaggtg accgtcaggc cgtggtcgat aagcacttcc
      241 gccacacgga catagtcctg gaccatgaag ttttcgatgt catcgtggct gaagcagatt
      301 tccgggttga tgcccttttc cactaccatc ggcaggtact tgcggtagag ggaatgaaag
      361 ggcatgttta cctgaaccct ctttagaatt ttctcctgca tggcgacgtc ccgcaaaaaa
      421 tgaagtaaag gccttttatc acaggaggtc gccttcatca accctatcaa cccccatcga
      481 tgggcccgga tggagaacga cattgacagg tttttgtgat tgcgctaaca taacaacaag
      541 ttaataacac acctcaagga ggtagccctg gtcaagaaag aattgagcac gagaaaggcc
      601 gttcttctca tcgtcaacac cattctcaag aagaaggcga cgaatgtcgt cgttctgaag
      661 gtaaagaagc tttcatccat cgccgactat cttgtcatct gcagcggaag ctcggaccgg
      721 caggtccagg ctatcgcttc ttccatcgaa gaagacatga aaaaagagac gaaattaccc
      781 ctcggggtcg aaggggatcg tatcgggaaa tggattctca ttgattacgg cgacgtcgtc
      841 atccatgtct tcttagagcc ggtaagagaa ttttatgacc tggagcgcct gtggtcggat
      901 gtcccccgga tggagatcag ggatgatgtg acggagataa cgagcctcga caagggcatg
      961 tgaagtgaag tccttcatga ccggtcttgc ggacctcatc ttccctcccg catgttgctc
     1021 ctgcggcgct ttgctgggga ggggtatgac gaccttctgc ggcgcctgtc tttccgaaat
     1081 ccgctttatc cgtgcaccac tctgcccggt ctgcggcatt ccttatccga atgaggagga
     1141 aatcaaccac cggtgtgcgg attgcatccg gtcgaaggaa tcattttccc ttgcgagggc
     1201 cgtaggggtg tacgatgccg ttcttctcga ggtgatccac aggttcaaat accggggccg
     1261 gatcaacctc ggcgaggtcc tgggtcgctt catggcggag tttcgctatg aagacctgag
     1321 aattgaggat tattcgctga ttgtgcccgt tccactccac aggagaaggt taaaagaacg
     1381 gggtttcaac caggccgtgg tcctggcgag ggagatttca cggcggcacg ccgtcccgat
     1441 ggatttcggt gtcttgaaga gaaagattta cacggaacct cagaccggtc tgggtaggga
     1501 cgacaggctc cgcaacatcc ggggggcctt tgaagtcgtg aggccggagg cggtgaagga
     1561 gaaaaaggta ctgctcatag atgatgttta cacgacggga agcacggtga gggagtgcgc
     1621 gagggttctg gggaaatgcg gcgcctcgga ggtggcggcg ttgacgttgg cgagggcggt
     1681 atgagacctg aggccgaagg ttgtggggta aagaagacga ggttcgagtg gtaaaagaaa
     1741 aaatacgctc atgggaagaa ctcaaaggtg aggttgaccg gctaaaggcc agggggaaga
     1801 agatcgtctt cacgaacgga tgtttcgata tactccatgt cggccataca cggtacctgg
     1861 gagaggcgaa aaagctcgga gacgtgcttg tcgttgctgt caacagcgat tcgtcggtaa
     1921 aggcgatcaa gggcgagaaa aggcctctcg tccccgaaac ggaaagggtc gaagtcgtcg
     1981 catcgctgga agctgtggat ttcgtaacca tttttgccga ggaaacgccg gtcacgctga
     2041 ttgaggagct caaaccgaac gtcatcgtca aggggggcga ttgggacgag aagaacgtag
     2101 ccggtaggga gtcggtgaga aaatggggag gaagggtggc gatcataccc caggttgaag
     2161 gcgtatcgac caccaacgtg gtggaaaaga taaggaaaat ttacgcttag ggaagggcaa
     2221 tttttttctt tacaaaggaa aaatgcttat tgtataaaag caaatttcag aataccaaca
     2281 gtttagtcag actccgagag aaaagatgtc atgaagcctg aaaaacttga gtactttaga
     2341 ggacttctaa atcaaaagat caacgagctc ctgggaggcg cggaaaaaac cgtctcagag
     2401 atgcttgacg agaaggagaa ttttcccgac cccaacgaca gggcgtccct cgagtcggac
     2461 aggaatttcg agttgcgtat ccgcgaccga gagcggaagc tcatcaagaa aatgcaggaa
     2521 gcgctcgagc gcatcgacga cgggacattc gggatatgcg agagatgcgg ggggccgata
     2581 tccgaaaaga ggttgatggc gaggccggtg acgacgctct gtatcgagtg caagacgaaa
     2641 gaagagaaga tcgaaaaaca aaggggagag tagtatccca tcgcatcagt cggttgcgtc
     2701 gaaccccggg gtagaatccg gggtttttta ttgtgccccg caggcaatta acccttgctt
     2761 cgagcattgc ttatggcata ttttcagcat cgccgggtcg tgtttcttgt tttggcgttt
     2821 tcgccccggc acgacactat cgggagaacc tacagagcat gtatgtggaa gtcgccgtcg
     2881 ccgttccctc ggaaaaaacc ttcacctatg ccgttcctga gaattttgcg ggagacatcg
     2941 ccctgggcaa acgggttctc gttcctttcg gcagaaaaag attgacgggc tatatcgtgg
     3001 gaatcctggg gcaggccgag cgtgaaggat tgaaagaaat acttgaagtt ctcgatgaaa
     3061 cttccttatt taaagaggat gattacgaat tttaccgctg ggccgcggca tactacatgt
     3121 atccactcgg taaggccctg gaagagatat tgccgggcgg gataaacatc gacagcggcc
     3181 gttgggtatg cctcgtgggc gacggcggcg ccgacggatt gaccgagagg gagcgtacga
     3241 tccttgatat cctcaggaaa aatcccggcg gcctctccgt cgcttctttg caaagagaag
     3301 cggactctcg tcagcttctt cgatccctca atcgtcttca gcaaaaaggg atgatccgta
     3361 tcgaagaaag gatggcgagg ccggcagtga cggcccggag agaaaaattc gtttcgatgg
     3421 cgccgggttg gtgcaacggc cgattatccg agaaggccgg ggcggtggtc caatacctct
     3481 cagaggcggg tgagacgacg ttttcggagc tacgccggcg gttcggcgtg acggcggata
     3541 ccgtgaggcg gctgggggaa aaaggagccg tcgtcgtaac cgaacgggag gtttatcgaa
     3601 aggcgtcggc cggaccgacc atcgccaaaa tggatgccgt gacactcaac agcgcccagg
     3661 aaggggctgc aaaggaaatt ctgaaaggcc tcctttccgg gaagttttcc ccgtttcttc
     3721 tccacggcgt cacggggagc ggcaagactg aggtctattt ccgggccctc gcagaggccc
     3781 ttgaccttgg gggaagcgtc atctatctcg tgcccgagat cgcgctgacg ccgcaattgc
     3841 tgagtcgcct cagggataga ttcaaggaac acgaaatggc cgtattgcac agcggcgtgc
     3901 cggcagccgt gcgttatgac cagtggagga tggttgcgcg gggtgacatc cggcttgttg
     3961 ttggtgcccg ttccgccctt tttgcccccg tccgggatct gagactgatc atcgtcgatg
     4021 aggagcatga caattcatac aaacaggatg aacggatgcc ttacaacgcc agggacctcg
     4081 cggtcgtgag ggcgaggcag agaggggcga cggtcatctt agggtcggcc acgccgagca
     4141 tacaatctta ctctaacgcc ctccggggga aataccgtca tctggtgctg acggaccggc
     4201 ccaagggtct ggggcttccc gaggtggagg tcctggatct acgcagagag cgggaggaaa
     4261 agggaaaaac tcccctcctt tcccgtccgc tcgtggaggc tatcggcgag acactcgaaa
     4321 cgggaaagca gacgcttctc ttcctcaaca ggcgggggtt ccataccttt cttttctgcg
     4381 ccgattgcgg gtacgtcttg aaatgcctca actgctccgt gtcgctgact ttccatgccg
     4441 cgtcaggtat tc
//
LOCUS       DAOTMX010000128         4447 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_15991_length_4447_cov_4.130692, whole genome shotgun sequence.
ACCESSION   DAOTMX010000128 DAOTMX010000000
VERSION     DAOTMX010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4447
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15991_length_4447_cov_4.130692"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="P5573_04765"
     CDS             <1..724
                     /locus_tag="P5573_04765"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRT27402.1"
                     /translation="PVLVPALNNSFASTAASASGLVAGLQALDRSDITVVAMAGDGGT
                     FDMGIQALSGAAERETDFIYVCYDNEGYMNTGTQRSSATPLGALTTTTPILTKQQHKK
                     DFIKIVEAHDVPYLATVSPAYPIDLYDKFVKAKKIKGTRYIHIYAPCPPGWTYPPKDT
                     VKMGRLAVETGVAVLFEVEDGKFRLTGRSKTLAERGNRLPVINYVEKQGRFRKMNIEQ
                     LNQLQAWVNRKWDEYMKRTEQD"
     gene            complement(737..2038)
                     /locus_tag="P5573_04770"
     CDS             complement(737..2038)
                     /locus_tag="P5573_04770"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HRT27403.1"
                     /translation="MYAVYNILLLLGTVILAPYYTLKILFTGKYRKSIGPKLGFVPQS
                     LYAGMEGSPRVWVHAVSVGEVTAAAPVVASLRDLFPEACLVVSTSTETGQLMARRIIP
                     EATSHIYYPLDIPFVVRKVIGRIRPDVFVLVETELWPNFIRLCKEAGAKVVMVNGRIS
                     PRSFKRYWATRFFWKKVLAMIDGIGVISDTDAQRLNAIGVPRSRIRVLGNAKFDGLAA
                     RVDERLKGEISGELGLREGEGVFVAGSTHEGEEEVVLRVYKRLLERHPGFKLVIVPRH
                     VERGGEVVNLVRKHGFEDVLSMSDIKRGQMPRGERVIVVDVIGELFKVYSLAAVVFCG
                     GSLVPKGGQNILEAAAWGKVVFYGPSMDDFREEKALLEGVGAGITVEDEEGLYAGIVS
                     MLSSPEKLTKSGAAGKRVVAANKGASMRYAALIEDIIANRS"
     gene            2173..2841
                     /gene="rpe"
                     /locus_tag="P5573_04775"
     CDS             2173..2841
                     /gene="rpe"
                     /locus_tag="P5573_04775"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HRT27404.1"
                     /translation="MKKIAPSILSADFSILGDEIRAVEKAGADLIHIDVMDGHFVPNI
                     TIGPSVVQALRKVTVLPFDVHLMVENPGDYVAAFASAGSNIITVHVEAAHHLHRTVEM
                     IKDMGVKAGVSLNPATPLVQVKEILPYVDLLLVMTVNPGFGGQKFIPGMLKKIREAKH
                     LVDSISGETSVEVDGGITVQNIGAVSEAGADIFVAGASVFTSGDYAGTIGRMKDILNG
                     RASC"
     gene            complement(2833..3897)
                     /locus_tag="P5573_04780"
     CDS             complement(2833..3897)
                     /locus_tag="P5573_04780"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HRT27405.1"
                     /translation="MNGKNGGNGGGEGLLEKLAPIVRRKRKILIITHDNPDPDGLASA
                     YALKYLLYAKWRVGSLIAYGGLVGRAENAALIKHLKIEVRHISGVNVRDFSVVALVDT
                     QPGTGNNPLSRAVEPEIVIDHHVPIYSRTLQAKFHDIRTDYGSTSSILTEYLMESGIE
                     NPDRKVATALFYGIKSDTSDLGREAGPKDMAAHLFLHPHVLFRVLSKISHPRVSIDYV
                     RAFAKALNNALIEDDVIVSDMGFIDNPASLGEMADFLLRVEGVRWVLCLGGFRGSIYF
                     SLRTTRRQANAGLTARKMVQDIGYGGGHDTTAGGKAGNDTEEIERYKMIADLLITRFL
                     REIKRRDFKGERLLSRETSA"
     gene            complement(3919..>4447)
                     /locus_tag="P5573_04785"
     CDS             complement(3919..>4447)
                     /locus_tag="P5573_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27406.1"
                     /translation="DHRRWQDELPNRSYAWIEDWFEKGLEAYRKSTGREPSSFGAPAW
                     LIDERALRVAGRYPFSYLSCTRAQKPFIHDVSHLPEVPSDLPCLEETGVEQGMAEVRK
                     ILEAGGTHVLPVHAEVEGGIWSGHFIELLQVVRLLDFRVMTLGGIRERLDLETLPVRR
                     FRLELLPGRAVPCAV"
BASE COUNT          964 a         1382 c         1222 g          879 t
ORIGIN      
        1 gccggtgctg gtgcctgccc tcaacaattc cttcgccagc acggcggcct ctgcgtcggg
       61 cctggtggcg ggtctccagg ccctggaccg gagcgacata acggttgtgg cgatggcagg
      121 ggacggaggg accttcgaca tgggaataca agccctgagc ggcgcggcgg agagggagac
      181 cgatttcatc tacgtctgtt acgataacga aggttacatg aacacgggga cgcagcgttc
      241 cagcgccacc ccgctgggcg cactcactac cacgacgccc atcctcacga agcaacagca
      301 caaaaaagac ttcatcaaga tcgtggaggc ccacgacgtc ccctatctcg ccaccgtttc
      361 tcccgcttat cccatcgacc tgtacgacaa attcgtcaag gccaagaaga tcaaggggac
      421 gagatacatc cacatctacg cgccttgccc gcccggatgg acgtaccccc cgaaggatac
      481 agtgaagatg ggaaggctgg cggtggagac gggcgtcgcc gtgctcttcg aggtcgaaga
      541 cgggaaattc cgccttaccg gcaggagcaa gaccctcgcc gaaaggggaa accgcctgcc
      601 agtgatcaac tacgtagaaa agcagggacg gttcaggaag atgaacatcg aacagttgaa
      661 ccagttgcag gcctgggtca accgcaagtg ggacgaatac atgaaaagga cggaacagga
      721 ctgagtctac ctatagttac gaccggtttg cgatgatgtc ctcgatcagc gcggcatacc
      781 gcatagaagc gcctttattg gcggctacga cgcgcttccc cgccgcaccc gacttcgtca
      841 acttttccgg cgaactcaac atcgagacga tcccagcgta aagcccctct tcgtcctcca
      901 ctgtaatacc ggcgccgacc ccttccagca gggccttttc ttcccggaaa tcgtccatgg
      961 aaggaccgta aaaaaccact ttaccccagg cggcggcctc gaggatgttc tgtccgccct
     1021 tgggaacgag actacccccg cagaagacga cagcggcgag gctgtagact ttgaacagct
     1081 cgccgatgac atccacaacg ataactctct caccgcgggg catctgcccc ctcttgatat
     1141 cgctcataga gaggacgtct tcgaaaccgt gtttacggac gaggttcacc acttccccgc
     1201 cccgctccac atgccgcgga acgatgacaa gcttgaagcc ggggtgacgc tccaggagcc
     1261 tcttgtacac acggaggacg acctcctctt ccccttcgtg ggtgctaccg gcgacgaaaa
     1321 ccccctcacc ttcccgaagg ccgagctccc ccgaaatctc ccccttcaat ctctcatcca
     1381 cccttgccgc gagtccgtcg aacttggcgt tccccagtac ccgaatcctc gaacgaggaa
     1441 ccccgatggc attgagtctc tgcgcgtctg tatcagagat gacgccgatc ccgtcgatca
     1501 tagccaggac cttcttccag aagaacctcg tggcccagta tcttttgaaa gaacggggcg
     1561 agatcctccc gttgaccata acgaccttcg caccggcttc cttgcagaga cggataaaat
     1621 tgggccatag ctccgtctcg acgaggacga acacgtccgg ccttattctt ccgatgacct
     1681 tcctaacgac aaaagggatg tcgaggggat agtagatatg ggatgtagcc tccgggatga
     1741 tcctccgggc cattaactgc cccgtttcgg tactcgtgga aaccacgaga caggcctcgg
     1801 ggaaaaggtc tcggagggag gcgaccaccg gtgccgccgc agtcacttcg cccacggaga
     1861 cggcgtggac ccacacccgg ggacttccct ccattcccgc gtaaagggac tgtgggacaa
     1921 accccagctt cgggccgatg ctcttcctgt atttacccgt aaaaaggatc ttcaaggtgt
     1981 aataaggagc caggatgacg gtccccagca gcaataaaat gttgtaaacg gcatacataa
     2041 caagtcgggt gaaatcgggt gtcgatgagt tcctcagcta tcaactgccg tcgggcctgt
     2101 caagcccctt ttctgcgttg acaacgcccc cgcctttcta tagattgacg gacacaggag
     2161 ggaaaaatct tcttgaagaa aatcgcacca tccatcctct cggccgactt cagcatccta
     2221 ggcgatgaaa taagggccgt ggaaaaagcc ggggcagacc tgattcacat cgacgtgatg
     2281 gacggtcatt tcgtccccaa catcacgata ggcccctcgg tcgtccaagc gctcaggaaa
     2341 gtaacggtcc tacccttcga cgtccatctg atggtcgaga atcccgggga ctacgtagcc
     2401 gctttcgcgt cagcggggag caacatcatc accgtccacg tggaggccgc ccaccaccta
     2461 caccgcaccg tcgaaatgat caaagatatg ggcgtaaagg ccggtgtctc gctgaacccg
     2521 gctacccccc ttgtgcaggt gaaggagata ctgccctacg tcgacctatt gcttgtcatg
     2581 accgtcaacc ccggcttcgg gggccaaaaa ttcatcccgg gaatgctgaa gaagataagg
     2641 gaggcgaagc acctcgtgga tagtatttca ggggagacat cggtcgaagt cgacgggggc
     2701 atcactgtcc agaacatcgg ggctgtttcg gaggccggcg ccgacatctt cgtggcgggg
     2761 gcctcggtgt tcacgagcgg ggactacgcg ggaacgatcg gcaggatgaa agatatcctg
     2821 aacggacggg cctcatgctg aagtttcccg gctgagaagc ctttcccctt tgaagtctcg
     2881 cctctttatc tctctgagga acctcgtgat aaggaggtcg gcaatcatct tgtagcgctc
     2941 gatttcttcg gtgtcgttac cggcttttcc ccccgccgtc gtgtcgtgac cgccaccata
     3001 tccgatgtcc tggaccatct tccgggccgt caggcctgca ttcgcctgcc tcctggttgt
     3061 gcgcaaagaa aagtagatac tcccccgaaa tccgccgaga cagagcaccc atctgacgcc
     3121 ctcgacgcgt agtaaaaaat ccgccatctc gccgagacta gcggggttgt cgatgaagcc
     3181 catgtcggaa acaatgacgt cgtcctcgat caaggcattg tttagggcct ttgcgaaagc
     3241 cctcacgtaa tctatcgaca ccctgggatg cgagatcttc gacaggaccc ggaaaagaac
     3301 atggggatgg agaaagaggt gtgccgccat atccttcggg ccggcttctc tcccgaggtc
     3361 gctcgtatcc gattttatcc cgtagaaaag ggccgtcgcc accttccggt ccgggttttc
     3421 gatccccgat tccatcaggt actcagtcag gatggacgac gtgctcccat agtccgtccg
     3481 gatgtcgtgg aacttcgcct ggagggtccg ggaatagata gggacatggt ggtcgatgac
     3541 gatctccggt tcgaccgcgc gcgacagggg gttgttaccc gtgcccggct gggtgtcgac
     3601 aagggcgacc acgctgaaat cccgcacatt caccccgctt atatgacgga cctcgatctt
     3661 gaggtgcttg atcaaggcgg cgttctccgc ccgccctact aaaccgccgt acgcgatcaa
     3721 gctcccaacc ctccattttg cgtagaggag gtacttgagc gcataggcgg acgccagccc
     3781 gtcggggtcg gggttgtcgt gggtgatgat caggattttg cgcttccgcc tcacgatcgg
     3841 ggcgagcttt tccaggagac cctcaccgcc gccgttaccg ccgttcttgc cgttcatcgt
     3901 cattcctcgt ccttggcctc acaccgcgca gggaaccgcc ctccccggca gaagttccag
     3961 ccggaatctt ctcaccggca gcgtctccag gtcaagacgt tcccttatcc ccccgagggt
     4021 catgacccga aaatcgagca gccggacgac ctgcagaagt tcgatgaaat gcccgctcca
     4081 tatgccgccc tccacctcgg catgaaccgg caggacatgg gtcccaccgg cctctaaaat
     4141 cttgcgtacc tcggccatcc cctgctctac ccctgtctct tcgagacagg gaaggtcgga
     4201 cggaacctcg ggcaagtgag agacgtcgtg gatgaagggt ttctgcgccc tcgtacaact
     4261 cagatagcta aaggggtacc ttcccgcgac gcggagggcc ctttcgtcga tgagccaggc
     4321 gggggcgccg aaggacgaag gctcccgtcc cgtcgatttc ctgtaagcct ccaggccttt
     4381 ctcgaaccaa tcctctatcc aggcgtaact gcggttcggg agttcatctt gccatctcct
     4441 gtggtcc
//
LOCUS       DAOTMX010000129         4444 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_16006_length_4444_cov_3.166097, whole genome shotgun sequence.
ACCESSION   DAOTMX010000129 DAOTMX010000000
VERSION     DAOTMX010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4444
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16006_length_4444_cov_3.166097"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(200..1018)
                     /locus_tag="P5573_04790"
     CDS             complement(200..1018)
                     /locus_tag="P5573_04790"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HRT27407.1"
                     /translation="MRWALYAFFAVVILAQMPGEAEAVNFYDGARAPEGLYLLTYTSF
                     YTADDLTDSHGNTVKSDYGLSKAEELLRFCYYTPNLVLTALAPFDYLEIDSRHQKSSG
                     LGDISVGAGYFLPIKSVDILPMLFIKLPTGKYDSTSAANVGTGQYDIKPTVFLYKAFG
                     DFSIDAAVKYFFRLENHDNHVSPGDELHLQCLLGYNVTDRFKLGPSFNWMVSRDKKLN
                     GVKVPNSARETFSAGADFYYRFSKFSVTFTYLYDAYAENSTKGSFFQLKTVFRF"
     gene            complement(1195..1947)
                     /locus_tag="P5573_04795"
     CDS             complement(1195..1947)
                     /locus_tag="P5573_04795"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRT27408.1"
                     /translation="MSFTQIICEKEDGIFWITLNRPEAMNALTPEMLEELREAMISAE
                     KDEEVRVVVVTGAGKAYCAGLDLKVLGARKLEKGEVGPIVDIPAKALINTVKNLSKPV
                     IAMVNGACITGGLELSINFDLIVASEDARFGDTHARWGLRPSWGLSQTFPRRVGLMKA
                     KELSFTARLFSAREAEEVGLVNRVVPADRLRQEVKSLAEGIMKNSADAIAAIKTLYNK
                     GIALTEAEGWKLEEEMHFEIRDTNERLQDFRK"
     gene            2109..2453
                     /locus_tag="P5573_04800"
     CDS             2109..2453
                     /locus_tag="P5573_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRT27409.1"
                     /translation="MRDFIKVTKALSDPNRVKIIKMLQKKEMCVCELQAALDIAQPTV
                     SKHLRVLEDAGLVSFSKDGLWVNYRLSDGRESPYAATLLGNLRHWLEDDPHIVELVGK
                     LPDIRREEIKKR"
     gene            2518..3564
                     /locus_tag="P5573_04805"
     CDS             2518..3564
                     /locus_tag="P5573_04805"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HRT27410.1"
                     /translation="MIEDLVVAGLLAIKDYVATHVLTCLVPAFLLAGAMVAFINQEAI
                     LDHLGERANKLKSFSMAALASFFVAACSCTVIPVSSGLYYSGAAIGVAFIILWVAPSA
                     NILSLIYTGNILGGEMVIARVVAALFMAFIVGWVMTYAFKNEKREVADFGGSKTQGGV
                     IARRHLILMVLILLSLLLPNYVVQTGSYLYKVTVWLAATAVASVYAWKALTVEEIKSL
                     LSESWWFVKLIFPLLLVGVFLVGVLGKLLPADWIKGWLGGNGVAASFFATLIGAVSYF
                     ATMTEAPFVDTLMKLGMGRGPALALLLTGPGLSLPNWIAIARVFGIKKSFVYVITIIV
                     VGTFVGWFFGNYVL"
     gene            3581..3820
                     /locus_tag="P5573_04810"
     CDS             3581..3820
                     /locus_tag="P5573_04810"
                     /inference="COORDINATES: protein motif:HMM:NF024590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRT27411.1"
                     /translation="MKIQVCGPGCARCIATEKNVIDACAALNLAADVSHVFDVAEFRK
                     LGVRVTPAVLVDGKIVVSGKIPTVEELKKILSEAK"
     gene            3831..4103
                     /locus_tag="P5573_04815"
     CDS             3831..4103
                     /locus_tag="P5573_04815"
                     /inference="COORDINATES: protein motif:HMM:NF013346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HRT27412.1"
                     /translation="MDMKVLATVFFSVFIAELGDKTQLATLAFAADKEVSKLTVFFGA
                     SLALVVAAGIGVLAGGFVAQHVSEKYLHYAAGLIFTGIGIFTLIRA"
     gene            complement(4160..>4444)
                     /locus_tag="P5573_04820"
     CDS             complement(4160..>4444)
                     /locus_tag="P5573_04820"
                     /inference="COORDINATES: protein motif:HMM:NF012626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HRT27413.1"
                     /translation="KERGERLSKLAGIMTTLPQVLINTPVARKKDLDKIPSVKKVLDE
                     AEDELRGRGRILVRFSGTQRMCRVMVEGPSEKVISAMAKRIASTIEEALK"
BASE COUNT          955 a         1169 c         1218 g         1102 t
ORIGIN      
        1 gaaacgatgg ggacaaaaat actaaccagt tgaacttatt cacagattat tccggacagt
       61 gatggtttaa atattgaaga attccgccgg ttggactgta aaatgcccaa attctgtctg
      121 tcaaggagat tctgagaacc catttccaac gaggagtttc tattttttac ggggtacaac
      181 agaccggcaa acggtcaggt cagaaacgga agaccgtctt cagttggaag aagctgccct
      241 tggtactgtt ctccgcatag gcatcataaa gataggtgaa ggtcacactg aatttcgaga
      301 acctgtagta aaaatcggcc ccggcggaaa aggtctcccg cgcgctgttc gggaccttaa
      361 cgccgtttag cttcttgtcc cgactgacca tccagttaaa ggacggccct aacttgaacc
      421 tgtcggtcac gttgtacccg agcaggcact gcaggtgaag ctcgtccccc ggcgagacgt
      481 gattgtcgtg gttctcgagc cggaagaaat acttgaccgc cgcgtcgatg ctgaaatccc
      541 cgaaggcctt atacagaaaa accgtgggct ttatatcgta ctggcctgtg ccgacattgg
      601 cggccgaggt cgagtcatat ttccccgtgg ggagtttaat gaagagcatg ggcaggatat
      661 caacgctttt gataggcagg aagtagccgg cgccgacgct tatgtccccg aggccggaag
      721 atttctggtg ccgggaatcg atttccaagt aatcgaaagg cgcaagggcc gtgaggacca
      781 agttgggagt gtagtaacag aaccggagca actcctccgc cttggagagc ccgtagtcgc
      841 ttttgactgt gttcccgtgg gagtcggtca ggtcgtcggc tgtgtaaaag gaggtgtagg
      901 tcagcaaata aagcccctcg ggggcccgag ccccatcata gaagttaacc gcttcggcct
      961 cgccgggcat ttgcgcaagt atgactacgg caaagaaagc atataaagcc cacctcatga
     1021 ttgtcaacct ccttttaaaa tggggttgac aatgccacaa gcgggctgat tttacaagga
     1081 gtaaaagccg cttttcccca ataacacgaa accgtcgact aagaggctta tttcgacggc
     1141 agtcgaggcc tcctttgggg gtccggtttt cggtgcagcg ggcaaggcgc gaatctattt
     1201 acggaaatct tgcagccttt catttgtgtc tctgatctcg aaatgcatct cttcttcgag
     1261 tttccagccc tccgcttcgg tcagggcaat ccctttattg tacagggttt ttatagcggc
     1321 gattgcgtcg gcactgtttt tcatgatgcc ctcagccaag gatttgacct cttgtctcaa
     1381 tctatctgcg ggaacgactc tgttcacaag gccgacttcc tcggcttccc ttgccgagaa
     1441 gagccttgct gtgaacgaaa gctcctttgc cttcatcagc cctacccgcc gcgggaatgt
     1501 ttggcttaag ccccaactcg gccggagtcc ccaacgtgca tgggtatccc cgaaccgggc
     1561 atcttcggag gcgacgatga ggtcgaaatt gatgctcaac tccaaacccc ccgtgataca
     1621 ggccccgttg accatcgcga tcaccggttt tgagagattc ttcacggtgt ttatgagtgc
     1681 ctttgcggga atgtcgacga taggccccac ttcacccttt tcgagcttcc tggcccctaa
     1741 gaccttgagg tccaggcctg cacagtaagc cttgccggca cccgtgacta cgaccaccct
     1801 gacctcttcg tccttttcgg cggagatcat ggcctccctc aattcttcga gcatttcggg
     1861 cgtgagtgca ttcatcgcct cagggcgatt cagtgtaatc cagaaaatcc catcctcttt
     1921 ttcacagatg atctgcgtaa acgacatccc ttgcctcctt tcggaaagaa gttctttgac
     1981 gcagaggcat tacacatagc cagggggtat cggtcaaggg ttttttaggc aacgagggcg
     2041 attcagtttt tcttgacatc tacccctttc ggttatatat agccatatag caatatgagg
     2101 tgatgggaat gagggacttc atcaaagtga caaaggcctt gtccgacccc aaccgcgtca
     2161 agatcatcaa gatgctgcag aagaaggaaa tgtgtgtctg cgaactccag gcggcactgg
     2221 acatcgccca accgaccgtt tccaagcacc tgagggttct cgaggatgcg gggcttgtct
     2281 ccttcagcaa ggatggcctc tgggtcaact acaggctctc cgacggaagg gaaagtccgt
     2341 atgccgcgac tctcctgggg aatctgaggc actggcttga ggatgacccc catattgtgg
     2401 aattggtcgg caagctcccc gacatcaggc gggaggagat caaaaagagg tgatacattt
     2461 tttgtctcgg gtatattgcc aaatggcaca atatgaatcg gataaggttg ttctatcatg
     2521 attgaggatt tagtcgtcgc cggtcttctg gcaatcaagg attatgttgc gacccacgtc
     2581 ctgacctgtc ttgtccccgc ctttctgctc gccggcgcga tggtagcttt catcaaccag
     2641 gaagcaatac tggaccacct cggagaaagg gcgaacaagc ttaaatcttt ttccatggcg
     2701 gcgcttgcca gtttttttgt ggccgcctgt tcctgcacgg tcatccctgt ttcaagcggc
     2761 ctttattaca gcggcgcggc catcggcgtc gccttcataa tcctgtgggt tgcaccctcg
     2821 gcgaatatcc tatcgctcat atatacgggc aatatcctgg gcggagagat ggtaatcgcg
     2881 agggttgtcg ccgccctttt catggccttt atcgtcgggt gggtcatgac ctatgccttc
     2941 aagaacgaga aaagggaggt tgcggatttc ggaggaagta agacccaggg aggagtcatc
     3001 gcccggcggc acctcatcct catggtcctc atcctccttt ccctgctgtt gcccaattac
     3061 gtcgtgcaga cagggtcgta cctgtataaa gtcactgtgt ggctcgcggc gacggcggta
     3121 gcgtcggtgt atgcctggaa ggcattgacg gtagaggaaa tcaagagttt actgagcgag
     3181 tcctggtggt tcgtcaagct catcttcccg ctcctcctcg tcggggtctt ccttgtcggc
     3241 gttctcggga agctcctgcc cgcagactgg atcaagggct ggctgggggg taacggcgtg
     3301 gcggcttctt ttttcgccac cctcataggc gcagtcagtt atttcgccac catgacggag
     3361 gcgccctttg tcgatacgtt gatgaagctc gggatgggca ggggaccggc gctcgcgttg
     3421 ctcctgacgg ggcctggtct gagtctgccc aactggatcg ccatcgcccg ggtcttcggg
     3481 ataaaaaaat cttttgttta tgtcataacc atcatcgtgg tgggaacctt tgtgggatgg
     3541 ttcttcggaa attatgttct ttaagtaagg aggtaagacc atgaaaattc aagtatgcgg
     3601 acccggctgc gcaaggtgca ttgcgacgga aaagaacgtc atcgatgcct gcgccgccct
     3661 gaaccttgcc gccgatgtct cccatgtctt cgatgtcgcg gaatttcgta aactgggcgt
     3721 ccgggtcacc ccggctgtcc tcgtggatgg gaaaatcgtc gtctcgggaa agataccgac
     3781 ggttgaagag ctgaagaaga ttctgtccga agcaaagtag gggtacaaag atggacatga
     3841 aggtactggc tacagttttt ttctctgtat tcatcgccga gctgggtgac aagacacaac
     3901 ttgccacatt ggcgttcgcc gccgacaagg aagtgagtaa actcacggtg tttttcggcg
     3961 catcgctcgc attggtcgtc gcggcgggta tcggggtcct ggcaggcggc ttcgtcgccc
     4021 agcacgtgag cgaaaaatat cttcactacg ccgctggtct catttttacc ggcataggga
     4081 tttttactct gataagggct tagaaaaact cttggcttcc ggtccgcgat cgattgcctt
     4141 ccaggaaggc ggccggagct tatttcaatg cctcttcgat ggttgaggcg atccgtttcg
     4201 ccatggcgga aatgactttc tccgacgggc cttctaccat gacgcggcac atccgctggg
     4261 ttccggaaaa tctcacgagg attcttcccc gaccccggag ctcgtcctcg gcctcatcga
     4321 ggaccttctt taccgatggg atcttgtcga ggtctttctt cctcgcaact ggggtgttga
     4381 tgagtacctg cggcagggtc gtcattatac cggcgagctt ggacagccgt tcgccccttt
     4441 cctt
//
LOCUS       DAOTMX010000130         4404 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_16176_length_4404_cov_3.671189, whole genome shotgun sequence.
ACCESSION   DAOTMX010000130 DAOTMX010000000
VERSION     DAOTMX010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16176_length_4404_cov_3.671189"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1042
                     /locus_tag="P5573_04825"
     CDS             <1..1042
                     /locus_tag="P5573_04825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydrogenase small subunit"
                     /protein_id="HRT27414.1"
                     /translation="MTAALMLPPLFRPVVARAAEAFGRLPVVWLHFAECTGCSESFLR
                     CSYPNVDDILLETISLEYHETLMAAAGYQAEQNLEKCMQDFAGKFICVIEGALPQGLR
                     GQYLRLGPKGKTGIQVAKEVVSKAAAVVCIGSCSAFGNIQAARPNPTDAAGVGKVLGI
                     KTVNIAGCPPNGVNFTGTVLHYLMFGDLPALDSLGRPIWAYGKRIHDVCERRPHYDAG
                     EFVEEWGDAGAKKGWCLYKVGCKGPYTYGNCGKVRYNDAISWPVMAGHGCIGCYEPGF
                     WDTMAPFEKPIDESTIGGGDRTADNIGLVLTGVTAAGIAAHAVASAAVHRKKGEAPGE
                     DKVKAAQEEKHG"
     gene            1035..2711
                     /locus_tag="P5573_04830"
     CDS             1035..2711
                     /locus_tag="P5573_04830"
                     /inference="COORDINATES: protein motif:HMM:NF012592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HRT27415.1"
                     /translation="MAKRIIIDPITRIEGHLRIEVELDEKNVIRDAWSSITLWRGIET
                     ILKGRDPRDAGLIVQRFCGVCTMVHYEASILACEDAFGVKPPPNARIIRNLMAGAWYL
                     GDHIMHFYHLHGMDWIDVVSALSANPAKAVELAKSYHPNPYNCSETHYKAVQERLTKF
                     VKSGRLGPFANAYWGNPSYKLPPEANLIILSHYLDALSVSKICGQMQAIFGGKNPHPQ
                     SLVVGGVTSVMDSLDPQRLGAFLFRLKDARNFVETAYIPDVLLAATYYKGEGLAGVGG
                     GVQNYLCYGGFPLDDDWSTTLFPRGVVKGRNLAKPLQLDESKITEEVTHSWYKGTKPE
                     HPYEGTTEPEYTGYDGNGYLKGNEKYSWCKAPRFDGEPYEVGPLARMVVGYALGDKRI
                     KPLVEGTLKKTGLPAEALFSTLGRTAARAIETKLVGDQIEGWVNELVANIKKGDTRTW
                     TRCDVPSKAAQGRGMTEPPRGALGHWIRIENKVIANYQAVVPSTWNCSPRDKAGKRGP
                     YEESLIGTKLAKADQPLEILRTIHSFDPCMACAVHIVDPKTNDVRKYRVA"
     gene            2730..3404
                     /gene="cybH"
                     /locus_tag="P5573_04835"
     CDS             2730..3404
                     /gene="cybH"
                     /locus_tag="P5573_04835"
                     /inference="COORDINATES: protein motif:HMM:TIGR02125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase, b-type cytochrome subunit"
                     /protein_id="HRT27416.1"
                     /translation="MGKIEEKREWSVAIRINHWAMAAAIVVLIVTGFYIADPFTVYQG
                     ETTYKMFMGEVRFWHLLFGIFLLFLFVWRIYLAFFSRFHADWKDFFVWIDLKGLINQV
                     RFYLLIDKEEPERDERYLYGPTQSLTYLGLLAIILCMVVTGLILMGAGYHAGLSGFAY
                     KVLRPVETMLGGLAMVRYIHHILTWCFVLFIPVHVYMAFWHDALLKQGTVSSMISGVL
                     FKRAEK"
     gene            3483..3977
                     /locus_tag="P5573_04840"
     CDS             3483..3977
                     /locus_tag="P5573_04840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HRT27417.1"
                     /translation="MDEKRITVLGLGNVLLGDEGFGVHFVRLFAERYRFPEGVRVVDG
                     GTLGYMLLDDICSCEHLIVVDAIKTDAEPGSIYRFTMDEMEHYLPPPTSAHEVQFQDV
                     LVKAELIGQVPEVVFLCITPGSYEEMNMELTPRLRERLPLMETFVLKELARFNIVPER
                     IDHA"
     gene            3970..4314
                     /gene="hypA"
                     /locus_tag="P5573_04845"
     CDS             3970..4314
                     /gene="hypA"
                     /locus_tag="P5573_04845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HRT27418.1"
                     /translation="MHEISLASSLLDIINEQAAKHRFNRVNAVKLSLGCASSVEPKAL
                     EFAFSVQSKGTIADGASLHFEMQPVVHYCFSCRRDHSAEVFSPVCAFCGSEDVILTGG
                     TEELRLLELDVD"
BASE COUNT          940 a         1267 c         1319 g          878 t
ORIGIN      
        1 catgacggcg gcactgatgc ttcccccgtt gttccgcccg gtggttgcga gggcagcgga
       61 ggccttcggc cgcctgcccg ttgtctggct gcatttcgcc gagtgcacgg gctgcagtga
      121 atccttcctc cgttgttcct accccaacgt cgacgacatt cttttagaaa ccatttccct
      181 cgaataccac gaaacgctca tggccgccgc cggttaccag gcagagcaga accttgagaa
      241 gtgtatgcag gactttgccg gtaagttcat ctgcgtcatc gaaggagcgc ttccgcaagg
      301 gttgagaggt cagtacctga ggctcggccc gaagggaaag acgggaatcc aggtagccaa
      361 agaagtggta tcgaaggccg ccgccgtggt ctgtatcggg agttgctcgg ccttcggcaa
      421 tatccaggcc gccaggccga acccgaccga cgccgccggt gtcggcaagg ttctcgggat
      481 aaagacggtc aatatcgccg gttgtccgcc caacggcgtc aatttcacgg gtacggtcct
      541 tcattacctc atgttcggcg acctgcccgc cctcgactcc ctcggacggc ccatctgggc
      601 ttacgggaag agaatccacg atgtctgcga gagacggccc cattatgacg ccggcgagtt
      661 cgtcgaggaa tggggagatg caggggcaaa gaaaggttgg tgcctctaca aggtcggctg
      721 caagggtcct tatacatacg gcaactgcgg gaaggtgcgg tacaacgacg ctatatcgtg
      781 gccggtcatg gccggccacg gctgtatcgg ctgctacgag ccgggtttct gggacaccat
      841 ggcgcccttc gagaaaccca tcgatgagag caccatcggc ggcggggacc ggacggccga
      901 caacatcggc ctcgtcctga cgggcgtgac ggcggcgggc atcgccgccc atgcggtggc
      961 cagcgcggcc gttcatcgga agaagggaga ggcccccggc gaagacaaag tgaaggcagc
     1021 tcaggaggag aaacatggct aaacgaatca tcatagatcc catcacccgt atcgaaggtc
     1081 atttacggat cgaggtcgaa ctcgacgaga agaacgtcat ccgcgacgcc tggagcagca
     1141 tcacgctgtg gcgcgggatc gaaacgatcc tgaagggacg ggacccccgg gatgccgggc
     1201 ttatcgtcca gcgtttttgc ggtgtctgca ccatggtgca ctacgaggcg agcatcctgg
     1261 cctgtgagga cgcctttggt gtcaaaccgc cccccaacgc caggatcatc cgcaacctga
     1321 tggcaggcgc ctggtacctg ggcgaccaca tcatgcattt ctaccatctc cacggcatgg
     1381 actggatcga cgtcgtcagc gccctttcgg caaatcccgc aaaggcggtc gaactcgcaa
     1441 aaagctacca cccaaatcct tataattgtt ccgagaccca ttacaaggcc gtccaggaac
     1501 ggctgaccaa gttcgtcaag tccggccggc tcgggccgtt cgccaatgcg tactggggca
     1561 atccttctta caaactgccc ccggaggcga acctcatcat cctgtcccat tacctcgacg
     1621 ccctgtccgt gtccaaaatc tgcggccaga tgcaggccat cttcggcggg aagaaccccc
     1681 atccacagtc cctggtcgtc ggcggcgtaa catccgtgat ggattcccta gacccccagc
     1741 gcctcggcgc cttcctcttc cgcctgaagg acgccaggaa cttcgtcgaa acggcttaca
     1801 tacccgatgt gctcctcgcg gcgacctatt acaagggaga aggccttgcc ggagttggcg
     1861 gcggcgtgca gaattatctc tgctacggcg gattcccgct cgacgacgac tggagcacga
     1921 ccctcttccc caggggcgtc gtgaagggaa ggaatctggc aaagccgttg caattagacg
     1981 agagcaagat caccgaggag gtgactcact cctggtacaa gggcacgaag cccgaacatc
     2041 cctacgaagg gacgacggag cccgaataca cgggctatga cggcaacggc tacctcaagg
     2101 gtaacgagaa gtacagctgg tgcaaggccc cgcggttcga cggcgagcct tacgaggtcg
     2161 gaccgctggc gcggatggtc gtcggttatg ccctcggcga caagaggatc aagccccttg
     2221 tcgaggggac gctgaagaaa acgggattgc ctgccgaggc gttattctcg acgctgggga
     2281 ggaccgcagc aagggctatc gagaccaaac ttgtgggaga ccagatcgaa ggttgggtga
     2341 acgagctcgt cgccaacatc aagaaagggg ataccaggac atggactcgc tgcgatgtcc
     2401 cgagcaaagc tgctcagggg cggggtatga cagagccgcc gcggggcgca ctgggacact
     2461 ggataaggat cgaaaacaag gtcatcgcca actaccaggc cgtcgttccc tccacctgga
     2521 actgttcgcc ccgcgataag gcgggcaagc gaggccccta cgaagaatcg ttgatcggaa
     2581 caaagctcgc caaggccgac cagccgctgg agattctccg gacgatccat tccttcgatc
     2641 cctgtatggc ctgcgcggtt cacatcgtcg acccgaagac gaacgacgtc cgtaagtacc
     2701 gggtggcttg agggaaagga ggtgctaccg tggggaaaat agaagaaaaa cgtgaatggt
     2761 cggtggccat tcggataaac cattgggcca tggcggcggc gatcgtcgtc ctgatcgtga
     2821 cgggattcta catcgccgac ccgttcacgg tctaccaggg tgagactact tacaagatgt
     2881 tcatgggcga ggttcgattc tggcatctcc tcttcggaat cttccttctt ttcctcttcg
     2941 tctggagaat ctacctggcc ttcttttcgc ggttccacgc cgactggaag gattttttcg
     3001 tctggattga cttgaagggt ctcataaatc aggtccggtt ttatctgctc atcgacaaag
     3061 aagaaccgga gcgcgacgag aggtacctct atgggccgac gcagtcgctc acgtacctcg
     3121 gccttctcgc cataatcctg tgcatggtcg taaccggcct catcctgatg ggagcaggct
     3181 accatgcggg gctttcaggt tttgcttaca aggtactaag gcccgtcgaa acgatgctgg
     3241 gtggacttgc aatggtgcgg tacatccacc atatcctgac ctggtgtttc gtcctcttca
     3301 tcccggtcca tgtctacatg gccttctggc acgacgcgct cttgaagcag ggaacggtat
     3361 cgtctatgat cagcggggtg ctcttcaaga gggctgaaaa gtgagtttcc atcctctaaa
     3421 acggccctga aaaggggaga gatgggggga tcttttcccc ccattttttg ttgtggtagg
     3481 ctatggacga aaagagaatc accgtcctcg gactcgggaa cgtccttctc ggcgatgaag
     3541 gtttcggggt ccattttgtg cgcctcttcg cagagcgata ccggtttccc gagggcgttc
     3601 gggtggtcga cggcgggacg ctcggttaca tgctgttgga cgacatctgt agttgcgagc
     3661 atctgatcgt ggtcgatgcc atcaagacgg acgccgagcc ggggtcgata tatcgcttta
     3721 cgatggacga gatggagcat tacctgccgc ccccgacgtc ggcccatgag gtacaatttc
     3781 aggacgtcct ggtcaaggcg gaactgatag gtcaagtgcc ggaggtcgtc tttctctgca
     3841 tcacgcccgg gagctacgaa gagatgaaca tggaactgac ccctcggttg agggaaagac
     3901 tgcccctcat ggagactttc gtcttgaaag agctcgcgag gtttaatata gtgcccgaga
     3961 gaatcgacca tgcatgagat ttcccttgcc tcctccctcc tggacatcat caacgagcag
     4021 gcggcgaaac atcggttcaa ccgcgtaaac gccgtcaaac tctctctcgg atgcgcctcc
     4081 tccgtggagc cgaaggcgct ggaattcgcc ttctcggtcc agtcgaaggg gacgatagca
     4141 gacggggcct ctctgcactt tgagatgcag cccgtcgtcc actactgctt ctcctgccgg
     4201 agagaccaca gcgcggaggt tttttccccg gtctgtgcct tctgcgggag cgaggatgtg
     4261 atcctcacgg gcgggacaga ggagctaagg ctcctggagt tggacgtcga ttaggaggaa
     4321 cgaataagat gtgcatcgcc ttccccggaa agatcatttc catcgatgat gaccgcgtcg
     4381 ccgtcatcga cataagcgga acaa
//
LOCUS       DAOTMX010000131         4388 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_16247_length_4388_cov_4.029541, whole genome shotgun sequence.
ACCESSION   DAOTMX010000131 DAOTMX010000000
VERSION     DAOTMX010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4388
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16247_length_4388_cov_4.029541"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="P5573_04850"
     CDS             complement(<1..537)
                     /locus_tag="P5573_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27419.1"
                     /translation="MENKRIIPIWLATVIVILMVILFSMLINRAREKEIVERFSRQQM
                     AIAKGLAAGIEDLIADVGKSMMIHSLRLTSGRKAIVSLREDVRVIFDSLGAKVKFVAA
                     DGADGSPIVYPSDYRQAFLTKRPEMQALMREVKRTGKPHVGDFLRKEGNVGAERIGED
                     ETLVVVAVPRFGQDREVIG"
     gene            complement(557..703)
                     /locus_tag="P5573_04855"
     CDS             complement(557..703)
                     /locus_tag="P5573_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27420.1"
                     /translation="MAWLACEKVFHIESGLTKLLISLGAAVGVYVLIAVKESREKTRK
                     GRDD"
     gene            1204..1578
                     /gene="atpE"
                     /locus_tag="P5573_04860"
     CDS             1204..1578
                     /gene="atpE"
                     /locus_tag="P5573_04860"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HRT27421.1"
                     /translation="MLKKFGKTLLTLFSAVVTLFVCSPVVFAAEALPTGEPYTKVIFA
                     VGAMIAAGIAIGGGAIGAGAGIGNAASGACQAVGRNPGVQGKILTTMLVGMAMAESIT
                     IYALVVSLVLLYANPYMRYFLG"
     gene            1631..2950
                     /locus_tag="P5573_04865"
     CDS             1631..2950
                     /locus_tag="P5573_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HRT27422.1"
                     /translation="MPDTKKDKQLFTVARVFFLLIVIPLSLMAILIANGIFKLGITAK
                     ERAVTVLDQKSQEEIKIRTINTADEVASFLSERKKDLLVATIIPATEAAFKQFVNENK
                     RNIWVKSNDKIVQEPALLYTEMALIDRNGNEQIKISNGQVVPKSKLVNVSNPANTLYK
                     SEDYFNKAKALNKGDIYVGRVTGWYVNRQEFEQQNKRFTGVIRFATPLFSKEGFAGVV
                     VLALDYRHLAGFTDHIIPTQAQLVFEADASTGNYAYMVDNKGFMISHPNDYHIAGLYK
                     DGRPVPPLTEKTAEEQVKKGEEVLNLNLLGFMDPNLPEIAKDAAAGNSGIKTYKFGGH
                     TKFVAYAPIKFFTDDYPKPGGFGWVGMGVDVEKWNELAVATSQKIEKEAKAWSTTIIL
                     ILIISVVILFLISALLARGISRSIAAEVPEGSQEVVDYYEDEEEDNK"
     gene            3140..>4388
                     /locus_tag="P5573_04870"
     CDS             3140..>4388
                     /locus_tag="P5573_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HRT27423.1"
                     /translation="MKENKFVLWFDEIELKDIPQVGGKNASLGEMRRELTKKKVNIPD
                     GYAITAEAYRYLLESAGITEEIKNILADLDTHDIENLSERGEKIRSLIYNAELPLDLK
                     KDILDAYKQLCKEYSDSKVDVAVRSSATAEDLPDASFAGQQETYLNVRGETELIDACK
                     KCFASLFTDRAISYRVDKGFDHLSVALSIGVQKMVRSDLASAGVIFSIDTESGFRDAV
                     LITGSYGLGELVVQGTVNPDEFYVFKPTLRKGFRPILQKKLGTKEIKMVYATETHEEA
                     TVIVPVPEADRRRFCLTDDEILRLAEWTMIIEDHYSAKAGFFKPMDIEWGKDGATGEL
                     FILQARPETVQSQKDVNVLESYRLLETKTPLVTGTAVGSKIGAGPVNIIENVDAIKSF
                     QKGQVLVTDMTDPDWEPIMKIAAA"
BASE COUNT         1178 a         1127 c         1075 g         1008 t
ORIGIN      
        1 gccgataacc tccctgtctt gaccaaaccg cggaaccgcg accactacca gtgtttcgtc
       61 ctcgccgatt ctttcagcac cgacatttcc ttcttttctt agaaaatcgc cgacatgggg
      121 cttcccagtc cttttcacct ctctcatcag tgcctgcatc tcaggcctct tagtcaaaaa
      181 ggcctgccgg tagtcagagg gatagacaat agggctaccg tctgcgccgt ctgccgcgac
      241 gaacttcacc ttggcaccta agctgtcaaa gattaccctg acatcttctc ttaaggagac
      301 gatcgccttc cgcccgctgg tcagccgcag ggaatggatc atcatacttt ttcccacgtc
      361 ggcgataagg tcttcgatcc ccgcggccaa ccccttggct atggccattt gctgacggct
      421 gaaccgctcg acgatttcct tttcccgcgc acgatttatg agcatggaaa aaaggattac
      481 catcaagatg acgatgacgg tcgccaacca tataggaatt attcttttat tttccataga
      541 tggagtcatg caccgctcaa tcgtctcgtc ccttccgtgt cttctcgcgg ctttccttca
      601 cagctatcag gacatagacg ccaacggcgg caccaagaga gatgagaagt ttggtgaggc
      661 cgctttcgat gtgaaaaact ttttcgcatg ctaaccaggc cacaaggtat gttacagcgg
      721 tcaatatttc ctgcatcttc tgatccactc caacttgagc taaaggtgaa agacaggacg
      781 aaaaaaacat actccatagc tttgcacagc actaacactt ctgccatttt aaatgatcgt
      841 gtacaaaagg atactatcgc cccgactgtc atttttcaat aaaaaacatc cctccgtggg
      901 cctacgttct ttcatcgcct catgacgttt catttaacat ttcgttaaat ttccctctcc
      961 agtgtaacga aacgtcaaac gcagtccgta taaacgcact cactacactt cgccgacggt
     1021 aataattagc cctatgtttg tccaacctta gccacatctg acgacttccg aagaccgagg
     1081 cacattcatt gcatataggg cgggcaactg aagagctttt ccttttcttt ggatacagaa
     1141 ttatgttaca aggaaaaggt tggtcattca ggttacttta ccccatgaaa ggagattgga
     1201 cagatgttaa agaaattcgg aaagacttta ctaacccttt tctcagccgt ggtaactctg
     1261 tttgtgtgtt caccggtagt attcgcggcg gaagcccttc cgacgggtga accttacacg
     1321 aaggtgatct tcgctgtagg tgcgatgata gctgcaggta tagcgattgg tggcggtgca
     1381 atcggcgccg gtgcaggaat cggaaatgcc gcaagcggcg catgccaggc tgtcggaaga
     1441 aaccccggcg tccagggtaa gatcttgaca acgatgctcg tgggtatggc aatggcggaa
     1501 tcgattacca tttacgccct ggtcgtttcg ctcgttctcc tctacgcaaa cccctatatg
     1561 cgttattttt tgggttaatt taacggtcga acgattaatt caaaactaac aaagttaggg
     1621 ggagaaggcg atgccagaca caaaaaaaga caagcagctt ttcactgtag caagggtctt
     1681 cttcctgctt atagtaatcc ccttatcgtt gatggcgatt cttatcgcca atggaatatt
     1741 caaacttggg attacagcaa aagaacgtgc cgtcaccgtt ctcgatcaaa aatctcagga
     1801 agaaataaaa atacggacca tcaatacagc agatgaggtc gccagcttcc tcagtgagag
     1861 aaaaaaggac ctgctggtgg ccaccatcat cccagccacc gaggcggcgt tcaaacagtt
     1921 cgtcaatgaa aacaaaagaa atatctgggt gaagtcgaac gacaaaatcg tccaggaacc
     1981 tgctctcctc tacacggaga tggcgctcat tgacagaaac ggcaatgaac agatcaagat
     2041 atcaaacggc caggttgtgc cgaagtcaaa attggtcaat gtgagcaatc cggccaacac
     2101 cctttacaag tcggaagact acttcaacaa ggcgaaggct ctgaacaagg gcgatatata
     2161 cgtcggccgt gtcaccggat ggtacgtcaa tagacaggag ttcgagcaac aaaataaaag
     2221 attcacgggg gttatccgct ttgcaacccc gttgttcagc aaagaaggtt tcgccggagt
     2281 ggtcgttctg gctcttgatt acaggcacct cgccggcttc accgatcata tcatcccgac
     2341 acaggcacaa cttgtttttg aggccgacgc ttccacaggt aattatgcct atatggtcga
     2401 caacaagggg ttcatgatct cgcacccaaa cgactaccat atcgcgggcc tgtacaaaga
     2461 cgggagaccg gttcctcctc tgacggagaa gacagccgaa gagcaggtca aaaagggaga
     2521 ggaagtcctg aacctcaatc ttctcggctt catggacccg aacctacctg agatagcgaa
     2581 ggacgcagcc gccggaaact ccggcatcaa gacatacaaa ttcggtggcc atacgaagtt
     2641 cgtcgcctat gcaccgatca agttcttcac cgacgactat cctaaaccgg gcggttttgg
     2701 ttgggtcggg atgggtgtcg acgtagaaaa gtggaacgaa cttgccgtcg caacgtcgca
     2761 gaagatcgaa aaagaagcga aggcgtggtc gacaacgatc atcctgatcc tgatcatatc
     2821 tgtggtcatt cttttcctga tctccgcttt actggctcga ggaatttctc ggtccatcgc
     2881 agccgaggtg ccggaaggtt cgcaggaagt ggttgactat tacgaagatg aagaagaaga
     2941 caacaagtag gtgattgatt tcaggtgatt gatttcatct gagcatacaa aaaaggccgg
     3001 gttctccggc ctttttttgt tttccggccg tggcgaattt cgcgtccatg atttccattt
     3061 gactagaatt taccgcgctg gtataaatgc ccaacttatc ttcaagatgc cgtacaaacc
     3121 aagaaacctg gaggaacaga tgaaagaaaa caaattcgtc ctttggttcg atgaaataga
     3181 gttgaaagat ataccccaag tgggggggaa aaacgcctcc ctcggcgaga tgcgcaggga
     3241 gctcacgaag aagaaggtga acattcctga cggttatgcc ataacagccg aagcctatcg
     3301 ttacctttta gaaagtgccg gcatcacaga ggaaattaaa aatatcttgg cggacctgga
     3361 cacccacgat attgaaaatc tgtcggaaag aggggaaaaa atccggtcgc tcatctacaa
     3421 tgccgaactg ccgctggacc tgaagaaaga catactggat gcatacaaac agctttgtaa
     3481 agaatactcg gacagtaaag tcgatgttgc ggtaagaagc tcggcaactg cggaagacct
     3541 accggacgca agcttcgcag ggcagcagga gacttacctg aacgtcaggg gcgaaaccga
     3601 gcttatcgat gcctgcaaga aatgcttcgc ttcccttttt accgaccggg cgatttccta
     3661 ccgggtggac aaagggttcg accacctttc cgtggcgctc tctatcggcg tgcagaagat
     3721 ggtgcgctcc gacctcgcct ccgcaggcgt tattttttcc atcgatacgg aatcgggatt
     3781 ccgtgacgcc gtcctgataa ccggctctta cggcttaggt gaactggtgg tccagggcac
     3841 ggtcaaccca gatgaatttt atgtcttcaa gcctacgctc cggaagggat tccggccgat
     3901 cttacagaag aagctcggca ccaaagaaat aaagatggtc tatgccaccg aaacgcatga
     3961 agaagccacc gtcatcgtgc cggttcccga agccgaccgg aggcgtttct gcctgacgga
     4021 cgatgagatt ctccggcttg ccgagtggac catgatcatc gaagaccatt attctgcgaa
     4081 ggcaggcttc ttcaaaccta tggacataga atggggcaaa gacggcgcta ccggcgaact
     4141 gtttatcctc caggcaaggc ctgagaccgt ccagtcacag aaggatgtca acgtcctcga
     4201 aagctatcgg ctcctggaaa ccaaaacgcc cttggtcacg ggaaccgccg ttggctcaaa
     4261 gataggggcc gggccggtca acatcatcga aaacgtagat gccatcaaaa gtttccaaaa
     4321 agggcaggtc ctggtgacgg acatgacgga cccggactgg gagcccatca tgaagatcgc
     4381 cgccgcca
//
LOCUS       DAOTMX010000132         4369 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_16329_length_4369_cov_3.476124, whole genome shotgun sequence.
ACCESSION   DAOTMX010000132 DAOTMX010000000
VERSION     DAOTMX010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4369
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16329_length_4369_cov_3.476124"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1131
                     /locus_tag="P5573_04875"
     CDS             <1..1131
                     /locus_tag="P5573_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HRT27424.1"
                     /translation="NFAAFWIGFSIFVLMVIAYYTSTLGLSNFMDYSSVFAFGLVAFG
                     MLGMGPVTIAVDSYGPVTDNAQSIYELSLIETIPNVESEIQTQFGFKPRMDVAKHYLE
                     ENDSCGNTFKATAKPVLIGTAVVGATTMIFSLILLLKKALGIQPEMILNLLNPYTLLG
                     LLCGGAVIYWFTGASTQAVVTGAYRAVAYIKKNIRLDDSASLSASTESSKEVVRICTI
                     YAQKGMINIFIAIFCFALAFAFLSAPITGPQAASFFISYLISIAVFGLFQALFMANAG
                     GCWDNAKKVVEVDLKEKGTPLHAAAVIGDTVGDPFKDTSSVALNPIIKFTTLFGMLAM
                     EIAIVPAMKGSATLVGLVFLLISLVFVYRSFYSMRIPIEASK"
     gene            complement(1262..2251)
                     /gene="moaA"
                     /locus_tag="P5573_04880"
     CDS             complement(1262..2251)
                     /gene="moaA"
                     /locus_tag="P5573_04880"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HRT27425.1"
                     /translation="MIDRFGREINYLRVSVTDRCNLRCVYCMPEEGISVMNHDDILRY
                     EEVLRVVRSSVGLGIVKVRVTGGEPLVRRGVIGFLSSLASLPGLKDLSLTTNGALLGS
                     MADDIFDAGVRRINVSLDSLNSVKYTRITRGGFLGDVLGGLERARRVGFSPIKINVVT
                     IKGLNEDEIIDFARLTIEQPYQVRFIELMPLGQAADEHESRYISNDEVHRRISRYFEL
                     EPLERKNKTDGPARIYRLRNGTGEIGLIGSISSHVCRSCNRLRLTADGRLRACLFSES
                     EIDLKRPLRNGCRDDELAGLIKGAVEEKPEGMDRPTGGGRPRRCMREMSSIGG"
     gene            complement(2293..3369)
                     /locus_tag="P5573_04885"
     CDS             complement(2293..3369)
                     /locus_tag="P5573_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HRT27426.1"
                     /translation="MNVYAVIMAGGKGTRFWPRSRESHPKHLLDILSSKSILRETTDR
                     IRPLVPLEQIFVVTGRLHCEKVRHDLDCIPPRNILVEPEGKNTAPCIGLAALHVRKED
                     PEGVMVVLSADHFIGKEESFLKDISVAWEMAGRGPYLVALGVKPTRPETGYGYLEIGS
                     PVSTIKRKIIYKVKSFHEKPDAEKAREFVKNGRFFWNSGIFVWKASTVLTAIERHLPD
                     LHEGLKEIGRVLGTPKEGDVVKEVYGRLEPISIDHGVMEKSPDVLMLKGAFQWSDVGS
                     WDALWEIRGKDEWGNVFAGEVINVRSRNSFVHSPKKLVAIVDVEDLIVVEDEDALLVC
                     RRGSSQGVKEVVEELEKKDLKRYL"
     gene            3553..>4369
                     /locus_tag="P5573_04890"
     CDS             3553..>4369
                     /locus_tag="P5573_04890"
                     /inference="COORDINATES: protein motif:HMM:NF013383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27427.1"
                     /translation="MDTNKVAIIGPGRLGTALAYKFGNNGKKVTLYYHDVDLCKAINT
                     EHLNPKHLTQDLASKLGGIDRVPRLSDKVFATNDLEWVVKEHDFIFLSMTITGLPEIL
                     NYLKPLIAKKRGTTCLISPIKGLASDEKTSDLITPSQMIHIHLQYLKHKYSVVCIGGP
                     FFDTDIAIGNPVCVTVAGKKRISNIVRGELMKFNRRELSSYYNFDIIGVEACGALKNI
                     VANLKGVTDCLNLGPSLPGTLFARSGVEIRSLSLLLGGSFQAFHSQAGVGDLYV"
BASE COUNT          986 a         1293 c          998 g         1092 t
ORIGIN      
        1 aacttcgccg ccttctggat aggtttttcc atcttcgtct tgatggtcat cgcttactat
       61 acaagcacgt tggggctgtc caactttatg gactactcgt cggtcttcgc gttcggtctc
      121 gtggcgttcg gtatgctcgg catgggtcct gttacgatcg ccgtcgacag ctatggtccc
      181 gtcacggaca acgcccagtc catctacgag ctttctctca tcgaaaccat tcccaacgtc
      241 gagtcggaaa tccagacgca gtttgggttc aaaccccgta tggacgttgc caagcattac
      301 ctcgaagaga atgacagctg cggcaacacc ttcaaggcga cggcaaaacc ggttctgatc
      361 ggtacggcgg tcgtcggggc cacaacgatg atcttctctc tgatcctgct tctcaagaag
      421 gccctcggca tccaaccgga aatgatcctc aacctcctta atccttacac acttctgggt
      481 cttctgtgcg gtggtgcggt catttactgg ttcacgggtg catcgacaca agccgtagtg
      541 acgggcgctt atcgtgccgt cgcatacatc aagaagaata tcaggctaga tgatagcgcc
      601 agcctgagcg cctcgacgga aagttcaaaa gaagtggtaa gaatctgtac gatctacgca
      661 cagaaaggca tgataaacat cttcatcgcg atcttctgct tcgccctggc ctttgctttt
      721 ctgtctgcgc ccattaccgg tccccaggct gcttcgttct tcatcagcta cctgatttct
      781 atcgccgtat tcggtctctt tcaggccctc ttcatggcca atgcaggtgg ttgctgggat
      841 aatgcaaaga aagtggtcga agtcgaccta aaagaaaagg gaacacccct ccatgcagcg
      901 gccgtcatcg gtgataccgt cggcgacccc ttcaaggaca catcgtcggt cgcgttgaac
      961 ccgatcatca agttcacgac cctgttcgga atgctggcca tggaaatcgc gatcgtcccg
     1021 gcgatgaaag gttctgctac tttggtgggc cttgtcttcc tgctgatctc tctcgtgttc
     1081 gtgtaccgtt ccttctactc catgcgtatc cccatagagg cttcaaaata gcaacaaaaa
     1141 ctcactaatc aatgacagaa aagggaggcg gaagcctccc ttttctgttg gtttttttat
     1201 gtgattagac acgcaattcg tgagccagag gcatctcgaa cagccttgtc gctacggttc
     1261 gttatccccc gatagacgac atctctcgca tacaccttct tggccttcct ccgcctgtcg
     1321 gtctgtccat gccctcgggc ttttcttcaa cagcgccctt tatcagaccg gcaagttcat
     1381 cgtcgcggca tccgttcctg agcggccttt ttaaatctat ttcactttca gaaaacagac
     1441 atgccctgag cctcccgtcc gcggtcaacc tgagtctgtt gcatgatcta cagacatggc
     1501 tgctgatcga gccgatcagg ccgatttccc ctgtcccgtt cctcaatcga tagatgcggg
     1561 caggtccgtc cgtcttgttt tttctctcca gaggttccag ttcgaagtac cggcttatcc
     1621 gacgatgtac ctcgtcgtta gagatatagc gggattcgtg ttcgtcggcg gcctgcccga
     1681 gaggcatcaa ctcaataaac ctcacctggt aaggctgctc aatggtcaac cgggcgaagt
     1741 cgattatctc atcttcatta aggcctttga tagtcacgac gttgatcttt atcggcgaaa
     1801 atccgacccg gcgcgcccgc tcaagcccac cgagtacatc gccaagaaac ccgcctcgcg
     1861 ttatccgcgt atacttgaca ctgttcaacg aatcaaggct gacattgatt cgtcttaccc
     1921 cggcatcgaa gatgtcgtcg gccatagacc cgagcaacgc accgttcgtc gtcagtgata
     1981 ggtctttaag ccccggcagg gatgcaagcg aagagagaaa accgatgacc ccccttctga
     2041 ccaggggctc accccccgtc acccgtactt ttacgatccc cagccctacc gaagaccgaa
     2101 ccacgcgcag cacttcttcg taccgcagta tatcgtcatg attcatgacc gatattcctt
     2161 cttcgggcat acagtatacg caccgcagat tgcagcggtc cgtcacagaa acacgtaggt
     2221 agtttatctc tcttccgaac ctatcgatca ttgccgcgtc tcagaaaaac cctcctcaca
     2281 ccccggcgtt ctttacagat acctcttgag atccttcttt tcgagctcct cgacgacctc
     2341 tttaacccct tgcgaagacc cgcgcctgca taccagcaag gcgtcttcat cctcgacgac
     2401 gatgaggtct tctacatcaa cgatagccac gagctttttc gggctgtgaa cgaatgaatt
     2461 cctggaccgt acgttgatga cttcgccggc aaacacatta ccccactcat ctttaccccg
     2521 tatctcccat aaagcatccc agctcccgac gtcgctccac tgaaaagcac ccttcagcat
     2581 caggacgtcg ggactctttt ccatgacgcc gtggtcgatc gagataggtt cgagtctccc
     2641 gtagacttct ttaaccacgt cgccttcttt cggtgtcccc aggacacggc ctatttcttt
     2701 caggccttcg tgaaggtcag gcaaatgtcg ctcgatggcg gtgagtaccg tcgacgcttt
     2761 ccacacaaaa ataccgctgt tccagaaaaa acgcccgttc ttcacaaatt cccttgcctt
     2821 ctcagcgtcc ggtttttcat ggaacgactt gaccttgtaa attattttcc tcttgattgt
     2881 ggataccggg ctccctattt ctaagtaccc gtaaccagtt tccggtctcg tcggtttcac
     2941 gccaagcgcg acaagataag ggcctctacc ggccatttcc caagcaacgg atatatcctt
     3001 gagaaaagat tcctccttcc cgataaaatg gtccgcagaa agcacgacca tcaccccttc
     3061 cgggtcttct ttccgtacgt gaagcgccgc aagcccgata caaggcgccg tgtttttccc
     3121 ctccggttcg accaggatat tacggggcgg gatgcaatca aggtcatgac gaactttttc
     3181 gcagtgcaat cgaccggtaa ccacgaaaat ctgttccagc ggaaccagtg gccggattct
     3241 gtcggtcgtt tctctcagga tggacttcga actcaggata tcaagcaggt gtttgggatg
     3301 cgactccctg ctcctcggcc agaacctggt ccccttgccc cccgccatga ttactgcata
     3361 aacgttcatg ctcttccatc actcctttgc ccttcatcct caaccgcgac gcagattacc
     3421 cctaatacat cgcaatttca aactattaat gatagacata cccgccaaga acgaatcgat
     3481 tttgtttgac attctgcgga ccttatacct atattaacgg gcttcttcaa ggtatgattt
     3541 cgggggtcgt tggtggatac gaacaaggtt gcgataatag ggccgggaag attgggcacc
     3601 gcactggcat acaaattcgg caataacggc aagaaagtca ctctatacta ccatgatgtt
     3661 gacctttgca aagccatcaa taccgagcat ctcaacccga agcacttgac gcaagacctg
     3721 gcctcgaaac tgggcggcat tgaccgtgtt ccccgtcttt ccgataaggt ttttgccaca
     3781 aacgaccttg aatgggtcgt caaggaacac gatttcatct ttctttccat gacgataacc
     3841 ggtctgccgg aaattctgaa ctacttgaag cctttgatcg caaaaaaaag ggggaccacc
     3901 tgtctgatct cacccataaa aggccttgcc tcggacgaaa aaacgagcga ccttatcaca
     3961 ccgtcgcaga tgatccacat ccatctgcaa tacctgaagc ataaatacag cgtcgtttgt
     4021 atcgggggac catttttcga cacggacatc gcgatcggca accccgtatg cgtcacagtg
     4081 gccggaaaga agcgtatctc caacatcgtt cgcggagagc tgatgaaatt caaccgccga
     4141 gagctatcct cttactataa cttcgacatc atcggggtcg aggcctgcgg cgccctcaag
     4201 aatatcgtgg ccaatctcaa gggtgttacg gattgtctca acctgggacc gtcgctcccg
     4261 ggaacactgt tcgcccgctc cggggtcgaa atcaggtcgc tttccctgct tctgggcggt
     4321 agtttccagg ctttccacag ccaggccggt gtaggagacc tctatgtaa
//
LOCUS       DAOTMX010000133         4366 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_16341_length_4366_cov_3.340756, whole genome shotgun sequence.
ACCESSION   DAOTMX010000133 DAOTMX010000000
VERSION     DAOTMX010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4366
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16341_length_4366_cov_3.340756"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /gene="tuf"
                     /locus_tag="P5573_04895"
     CDS             complement(<1..906)
                     /gene="tuf"
                     /locus_tag="P5573_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HRT27428.1"
                     /translation="MAKKKFERTKPHLNIGTIGHVDHGKTTLTAAITKQLAKKGFAEF
                     RSFDSIDNAPEEKERGVTINITHVEYETPKRHYAHVDCPGHADYIKNMISGAAHMDGT
                     ILVVAASDGPMPQTREHILLARQVQVPSIVVFMNKVDLVDDPELLDLVELELRELLNQ
                     YEFPGDDIPIIRGSALKALEAEDPDSPDVKCIMELMDAVDSFIPEPVRDLDKPFLMPV
                     GDVFTISGRGTVVTGRVDRGIIKTGEEVEIVGIRPTIKTVCTGVEMFRKTLDEGRAGD
                     DVGLLLRGTKREEVERGQVLAKPGSI"
     gene            complement(1009..1479)
                     /gene="rpsG"
                     /locus_tag="P5573_04900"
     CDS             complement(1009..1479)
                     /gene="rpsG"
                     /locus_tag="P5573_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HRT27429.1"
                     /translation="MPRRREVAKREVQADPKYNNKLVAKFINNLMERGKKSTAESILY
                     GAFDIIEKKLKEAPLEVFEKAVNNVKPVIEVKSRRVGGSTYQVPTEVVPSRRVALGIR
                     WLITNSRERSEKTMREKLAAEFIDAANNRGASIKKREGVHKMAEANKAFAHYRF"
     gene            complement(1494..1865)
                     /gene="rpsL"
                     /locus_tag="P5573_04905"
     CDS             complement(1494..1865)
                     /gene="rpsL"
                     /locus_tag="P5573_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HRT27430.1"
                     /translation="MPTVNQLVRKGREKIAKKAATPALAGSPQRRGVCVRVYTTTPKK
                     PNSALRKVARVRLTSGYEVTSYIPGIGHNLQEHSVVLVRGGRVKDLPGVRYHIVRGTL
                     DSVGVQDRKQGRSKYGAKKPS"
     gene            complement(1918..>4366)
                     /locus_tag="P5573_04910"
     CDS             complement(1918..>4366)
                     /locus_tag="P5573_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRT27431.1"
                     /translation="KQIPFDAINKVMNKKELANLIDHCYRLCGDKTTVLLADRLKDLG
                     FKYATVSGASFAIHNLIIPSSKKDIVNDADRDVLKIQKRYMDGLITDGERYNMVIDIW
                     AQVTEKIAADMLDGIGTEEIESPDGKTVRSQSFNPVYMMADSGARGSNVQIRQLAGMR
                     GLMAKPSGEIIETPITANFREGLTVLQYFISTHGARKGLADTALKTANSGYLTRRLVD
                     VAQDCIITEQDCGTVDGIYVNALMEGGEIIEPAGERVLGRVAHEDIRDPFTGEILVHA
                     NQAIDEVLAEKIDGAGIESVNIRSVLTCKSKHGVCAMCYGRDLAHGHLVNIGEAVGII
                     AAQSIGEPGTQLTMRTFHIGGTAKFDEHSTLDARHDGVIRIVNLDQGRGDLALCKDGR
                     ELVVMRRNGEVHVLDEQGRSRGKYTVPYGAHLKLKEGEPVKKGDRIAEWDPFSIPILA
                     EVDGAVKFGDIIEGKTMQEQVDEVTGLSRKVIVEFKGGDLRPRISLKDDKGKTAKVPG
                     TNAVARHFLSVGVNIVVSEGDHVVAGDILAKIPRETQKTKDITGGLPRVAELVEARKP
                     KDYAVISEIDGVVTYGKDAKGKRKVLVLPDVGEEKEYLIPKGKHISVQEGDRVSAGEP
                     LMEGVQNPHDLLSIKGEKALARYLVDEIQEVYRLQGVKINDKHLEVIVRQMLRRVKVT
                     NPGDTSFITDEQVERYRFQEENRLVMERGGNPAIGEPMLLGITKASLSTQSFISAASF
                     QETTRVLTEASLAGKVDYLRGLKENVIMGRLIPAGTGLVMYRKLGIQAEEDVQYVEDL
                     PSDEKIDEKTLDNQGVN"
BASE COUNT          843 a         1355 c         1084 g         1084 t
ORIGIN      
        1 aatgctgccc ggcttcgcca gcacctgacc gcgctccacc tcttcacgct tcgttccccg
       61 aagcagcaga cccacatcgt cgccggctct tccttcgtcc aaggtcttcc ggaacatttc
      121 cacacccgtg cagaccgtct tgatggtcgg ccgaattccg acgatctcga cttcttcgcc
      181 ggtcttgatg attccgcggt caaccctgcc cgttacgacg gtaccgcgac cggaaatggt
      241 gaagacatca ccgaccggca tcaggaaggg cttgtccaga tccctcacgg gctcgggaat
      301 gaagctgtcc acggcatcca tgagttccat gatgcacttt acatccggag aatcgggatc
      361 ctccgcttcg agagccttca gagccgagcc acggatgatc ggaatatcgt cgccgggaaa
      421 ctcgtactga ttcagcagct cgcgaagttc cagctcaacc aggtccagca attcgggatc
      481 gtcgaccaga tcgaccttgt tcatgaagac aacgatggaa ggcacctgca cctgccgcgc
      541 caggaggata tgctcccggg tctggggcat ggggccatcg gaggcggcga cgacaagaat
      601 cgttccgtcc atgtgggcgg cgccggaaat catgttcttg atgtagtcgg cgtggccggg
      661 gcagtcgaca tgagcgtaat gccgcttcgg agtctcgtat tcaacatggg taatgttgat
      721 ggtaacgcct ctttccttct cctccggagc gttatcaatc gaatcgaaag acctgaattc
      781 cgcaaatccc tttttcgcca attgcttcgt aatggcggcg gtcaaggttg tcttgccgtg
      841 atcaacgtga ccaatggttc cgatattaag atgcggcttc gtcctttcaa actttttctt
      901 agccatttta atccccctcc ttcaggttta aatgattttt tctttaatga ttgatcgccg
      961 atttaatcgg tccgtacctg cttctaaaca ctcgaaatcc gcatgacgtc aaaaccggta
     1021 atgcgcgaaa gccttgttcg cctccgccat cttgtgcaca ccttcccgct tcttgataga
     1081 ggctccccgg ttgttggccg cgtcgataaa ctccgccgcc agtttttctc tcatcgtctt
     1141 ctcggaacgc tcccgcgagt tggtaatcag ccaccggatg cccaaggcaa ctcttctcga
     1201 gggaaccact tcggtcggca cctgataggt cgatccgccc actctcctcg atttgacctc
     1261 gatcaccggc ttgacattgt tgacggcctt ttcgaacacc tcaaggggcg cttccttcaa
     1321 tttcttttca atgatgtcga aggcaccgta caagatggac tcggcagtac ttttcttgcc
     1381 gcgctccatc agattattaa tgaatttggc gaccagcttg ttgttatatt tgggatccgc
     1441 ctggacctca cgcttggcta cttctcttct tctcggcatt ttacagtctc cgctcagctc
     1501 ggttttttcg caccatactt ggacctaccc tgtttccgat cctgaacccc gacggaatcc
     1561 agggttcctc ggacgatatg ataccggaca ccgggtaaat ccttcactct gccgcctctg
     1621 accagcacca cggagtgttc ctgcaaatta tgccctatac cgggtatata agaagtgact
     1681 tcataaccgc ttgtcaaacg caccctcgcc acttttcgca gagccgaatt gggcttcttg
     1741 ggcgtcgtcg tgtaaaccct cacacaaaca cccctgcgct ggggcgaacc ggcgagcgca
     1801 ggcgtcgccg ccttttttgc gattttctcc cgccccttcc tgaccaactg atttactgta
     1861 ggcattcttc ctcccgcaac ctaatctata cctgacgcaa tgaagctcgt gtcaatatca
     1921 attcacaccc tgattgtcaa gcgttttctc atctattttt tcatccgacg gcaggtcctc
     1981 aacgtactga acatcctcct ccgcctgaat cccgagcttc ctgtacatga ccaaacccgt
     2041 gccggcggga atcagccggc ccatgatcac gttttccttg agacctctca aatagtccac
     2101 tttgccggcc aagctcgcct ccgtgaggac cctcgtcgtc tcctggaatg aagccgccga
     2161 gataaaactc tgcgtggaaa gcgacgcctt ggtgattccc agcagcatcg gctcgccgat
     2221 ggcggggttt ccgccccgtt ccatgaccag cctgttctct tcctggaaac ggtaccgttc
     2281 cacctgttca tccgtgatga agctcgtatc cccgggattg gtcaccttga cccggcggag
     2341 catctgtctc acgatgacct caagatgctt gtcgttgatc ttgacgccct ggagccggta
     2401 gacttcctgg atttcatcaa ccaggtaccg ggcaagggcc ttttcaccct tgatgctcaa
     2461 aaggtcgtga ggattctgaa ctccctccat gagaggttcg cccgccgaaa cccggtctcc
     2521 ttcctggaca ctgatgtgct tccccttggg gatgagatat tccttttcct caccgacatc
     2581 cggcaggacc agaaccttcc gctttccctt tgcgtctttc ccgtaggtca caaccccgtc
     2641 gatttcgctg atcaccgcgt aatccttcgg ctttcttgcc tcaaccagct cggcgacccg
     2701 gggcagacca ccggtgatgt ccttggtttt ctgggtctcc cggggtatct tggcgagaat
     2761 gtctcccgcc acaacgtgat ctccttcgga cacgacaatg ttcacgccga cggacaggaa
     2821 atgccgggcc acggcattgg ttcccgggac cttggcagtt ttccccttgt catccttgag
     2881 ggaaatacgg ggacgcagat cgccgccctt gaactcgaca atgactttac gggacaggcc
     2941 ggtcacctcg tccacctgtt cctgcatggt ctttccttca atgatatcgc cgaacttaac
     3001 ggcaccgtcg acctcggcca gaatcggaat ggagaaggga tcccactcgg caatgcggtc
     3061 ccctttcttc accggctctc cctctttcag cttcagatgc gcgccatagg gaacggtata
     3121 ttttccgcgg ctccgtccct gttcatccag cacatggact tcaccgttcc gtcgcatcac
     3181 caccagttca cggccgtcct tgcacagggc cagatcgccc cttccctggt cgaggttgac
     3241 aatccggatc accccgtcat gacgggcgtc aagggtggaa tgctcgtcga atttcgccgt
     3301 accgccgata tggaacgttc tcatcgtcaa ctgggtgccc ggctcaccga tggactgggc
     3361 agcgataatc ccgacggcct caccgatatt gacgagatgt ccgtgggcca gatcccgtcc
     3421 gtaacacatg gcacagacgc cgtgtttgga cttgcaggtc agaaccgacc ggatgttgac
     3481 gctttcgatt cccgcaccgt cgatcttttc cgcaagcact tcatcgatgg cctggttggc
     3541 gtggaccagg atttccccgg taaagggatc ccggatatct tcatgggcca cccgtcccag
     3601 caccctttcg ccggcgggtt cgataatttc accgccctcc atgagggcat tgacataaat
     3661 gccgtcgacg gtaccgcaat cctgctccgt gatgatgcag tcctgtgcaa catcgacgag
     3721 cctacgggta agataacccg agttggccgt tttcagggcc gtatcggcga gaccttttcg
     3781 cgcaccgtgg gtggatatga agtattgaag gaccgtcaaa ccttcccgga aattggccgt
     3841 gatcggggtc tcgatgattt cacccgacgg cttggccatg agccctctca tccccgccag
     3901 ctgcctgatc tgaacgttgc tgcccctggc gccggaatcg gccatcatgt agacgggatt
     3961 gaagctctgg gatctgaccg tcttgccgtc ggggctttcg atctcctccg tcccgatgcc
     4021 gtcgagcatg tcggcggcga tcttttccgt cacctgggcc cagatgtcga tcaccatgtt
     4081 gtagcgctcc ccgtccgtga tcagaccgtc catgtatctt ttctggatct tcagaacgtc
     4141 ccggtcggca tcgttgacga tatccttctt gctcgatgga ataatgagat tatgaatggc
     4201 gaaggaggcg cctgaaaccg tcgcatactt gaagcccaga tccttcaggc gatccgccag
     4261 aagaaccgtc gtcttgtcgc cgcagaggcg gtagcagtgg tcgatcagat tggccagttc
     4321 cttcttgttc atgaccttgt tgatggcgtc gaagggaatc tgtttc
//
LOCUS       DAOTMX010000134         4360 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_16366_length_4360_cov_3.602323, whole genome shotgun sequence.
ACCESSION   DAOTMX010000134 DAOTMX010000000
VERSION     DAOTMX010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4360
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16366_length_4360_cov_3.602323"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1474
                     /locus_tag="P5573_04915"
     CDS             <1..1474
                     /locus_tag="P5573_04915"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRT27432.1"
                     /translation="LAVELASLYMRNGQFEEAAALCEKSLLHNQNYLDLHLLLGGIYF
                     EMKDFERAEKEFREAIRVDPKTLESYLYLSVIFAEEKKYGESIGVLKELLRVDPTNLM
                     GTYYLAKRYAEMKAYGEAEFWYKKTLNIKPDFESAVIDLGVVYEAQHKFNEAVELYKK
                     YIESHPGRIDMKLRLGKAYLQDRKYLESAKVMDEILRIDPTNRDVRLTMGMVVFFSGK
                     DYDRAAEEFRTLLKQDPFEHRARYFLALSYGEKKLYTEALVELDKIPEASDLYDDARI
                     QAALILRKIGKPGEAVAVIKDALQKKKGDPDLFNFLSVLYQEEGKLAEAEQALKEGLV
                     FSPRNADLRYRLGVIYEATNRFDESIKEMEEVLKLDPNHADALNFIGYSVADRGVKLE
                     EAEKMIKRALQLKPGSGYIIDSLGWVYFKQNKNDLALKYLKEAASLMPEDPLIQEHLG
                     DIYVRMGMIKEAIAAYRTALKLNPRQESAATKLDRLLKKK"
     gene            1564..2301
                     /locus_tag="P5573_04920"
     CDS             1564..2301
                     /locus_tag="P5573_04920"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HRT27433.1"
                     /translation="MKKSPPAPGRELPDPGEIIERLSGKHLVGMDLKGLARVTFRSPE
                     GVFASTCLIAARLPSSLRVETFPPIGPPNLFLSLNEKGIKVYVPDQGAFYIGGATKEN
                     VTRFLPFPLEVEEMIPLMMGMLPPSFCRERTVLKGRKADELYRIDADRGGGTLSCFVD
                     LARGELMRVESKDSGGRLIYAADLRNYRPVEGVALPQDVELHHEEGDLKEWSLRVSFS
                     DVRLSPGEDKDLYDLEVPSGVKLLYLD"
     gene            complement(2305..3069)
                     /gene="ybgF"
                     /locus_tag="P5573_04925"
     CDS             complement(2305..3069)
                     /gene="ybgF"
                     /locus_tag="P5573_04925"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HRT27434.1"
                     /translation="MFFLVGGCATSQDLRRMNQNLDQKITVLQEETVKLQKQLDERRE
                     AEKSLRKVQADTGADLIALRGDIQALRGTVEELRRDIDTMRADRKERDARLNDLALRI
                     SFIENFIGVGKKADGDDKQGVQHGRNNKNGRDTLYSSAYKDFKEGRYEEARKGFQKYI
                     EAYPNSDLSDNAQFWLGECYYLENNFEKAILEYEKVIKNYPNGDRVPHAILKQGLSFL
                     RLGDKESAKLLFQQVIKDYPNTNQANIARAKLSEMK"
     gene            complement(3124..3744)
                     /gene="pal"
                     /locus_tag="P5573_04930"
     CDS             complement(3124..3744)
                     /gene="pal"
                     /locus_tag="P5573_04930"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HRT27435.1"
                     /translation="MRRNVVLIGLSILILAFGFVMFSGCAKKVVKEAAEEKTTTVQKE
                     AAPATPAAPAETEEQRLAREKALREAALKEQQEKERMLAEQKAKEALAAAIAEIKDIH
                     FDFDKYDLKPEARDMLKKLADILLAEKTATVLIEGHCDERGTVEYNLALGEKRALSAM
                     KYLVSLGVEKERIKTISYGKERPLDPRHNEEAWAKNRRAHFVVTVK"
     gene            complement(3818..>4360)
                     /locus_tag="P5573_04935"
     CDS             complement(3818..>4360)
                     /locus_tag="P5573_04935"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HRT27436.1"
                     /translation="QGRKILLTLSKGGSHEIYTMDLNHGTATRVTSNYSINVSPVWSP
                     DGKRIAFVSDRGGSPQIYIMDADGSNQRRLTYQGNYNTSPAWAPKGGRIAFEGMVGGT
                     FQVFVIDEDGGNLRQLTSSGRNESPSWSPDSRYLVYTSRLNGRHRLCVMNANGSNVRI
                     LHEGMGEYMNASWSPHLKLY"
BASE COUNT          983 a         1226 c         1127 g         1024 t
ORIGIN      
        1 cctcgccgtg gaactcgctt ccctatacat gaggaacgga cagttcgaag aggccgcagc
       61 actctgtgaa aaatcacttc tccacaacca gaactacctc gacctgcatc ttctcctagg
      121 cggcatctat ttcgagatga aggatttcga gcgggcggaa aaggagtttc gcgaggccat
      181 ccgggtcgac ccaaaaacgc tggaatcgta tctctatctc agcgtaatct ttgccgaaga
      241 aaaaaaatac ggcgaatcga taggcgtgct gaaagaactc ttgcgggtag acccgacaaa
      301 ccttatgggg acttactatc ttgcaaagag atacgcggaa atgaaggcct acggggaggc
      361 cgagttctgg tacaaaaaga ccctgaacat caaaccagat tttgaatccg ccgtcataga
      421 cctcggggtg gtttacgagg ctcagcacaa gttcaacgaa gccgtcgaac tttacaagaa
      481 atacatcgaa tcccatccgg gtcggatcga tatgaaactg aggctcggta aggcctacct
      541 ccaggaccgg aagtacctgg aatcggcgaa ggtcatggat gagatactga ggatcgaccc
      601 cacgaaccgc gacgtacgcc tgactatggg catggttgtt tttttctccg gaaaagacta
      661 cgaccgggca gccgaagagt tcaggacgct gctgaagcag gacccctttg agcaccgcgc
      721 ccggtatttt ctcgctcttt cttacggaga aaaaaagcta tacaccgaag cccttgtcga
      781 gttggataag attcccgagg catcggacct ttacgacgac gcccggattc aggcggcctt
      841 gatcttgaga aagatcggta agcccggtga ggccgttgcc gtgataaagg acgctctgca
      901 gaaaaaaaaa ggtgaccccg accttttcaa ttttctgtcc gtcctctacc aggaggaagg
      961 gaagttagcg gaagccgagc aagccctgaa agaagggctt gtcttttcac cgaggaacgc
     1021 ggacctccgc taccggttgg gcgtgatata cgaagctacc aatcggttcg acgaaagcat
     1081 caaagaaatg gaagaggtcc tgaaattgga cccaaatcat gccgacgcct taaatttcat
     1141 cggatacagc gttgcggacc gcggggtcaa actcgaggaa gccgaaaaga tgatcaagag
     1201 ggcgcttcaa ttaaagccgg gaagcggata cataatagac agtctcggct gggtctattt
     1261 caaacagaac aaaaacgacc ttgccctcaa gtatctcaag gaagccgcgt cgctcatgcc
     1321 tgaagacccg ctcatccagg aacacctggg ggacatttac gtcaggatgg ggatgataaa
     1381 agaagccata gcggcttacc gtacggcatt gaagctcaac cccaggcagg aatccgccgc
     1441 tacgaagctc gatagactcc tgaagaaaaa gtgaataccg gtacccttct caatttctca
     1501 aattctactc tcatgttgtg tctcgcagcc ctctgcctcg tctccctctc aacgggttgt
     1561 acaatgaaga agtcgccgcc cgcgccgggc cgtgaactcc ccgaccccgg agaaatcatt
     1621 gaacgtctct ccgggaaaca tctcgtcggc atggacctga aagggctggc ccgtgtgaca
     1681 ttccgctcac cggagggcgt attcgcgtcg acgtgcctca tcgcggcaag gcttccctcc
     1741 tcgcttcggg tggagacatt cccccccata ggcccgccga acctttttct ttctctcaat
     1801 gagaagggaa tcaaagtata cgtgcccgac cagggcgcgt tctatatcgg aggggcgaca
     1861 aaggagaacg tcacccgttt tttacccttt cctttggaag tcgaggagat gattcctttg
     1921 atgatgggga tgttgccgcc gtccttttgc cgagaaagga cggtccttaa aggaagaaag
     1981 gccgatgagc tgtaccggat cgatgccgac agggggggtg gaacgctgtc ctgctttgtg
     2041 gaccttgccc gcggggagct gatgcgggtc gaatccaagg attcgggggg caggttgatt
     2101 tatgcggcag acttaagaaa ctacaggccc gtcgaaggag tggcccttcc gcaagacgtg
     2161 gaactccatc atgaggaagg ggatctgaaa gagtggtctc tccgggtcag cttttccgac
     2221 gtccggctct cgcccggaga ggacaaggac ctttatgacc tcgaagtccc ctcgggcgtc
     2281 aagctcctgt accttgattg aatactactt catttccgag agtttagccc gcgcaatgtt
     2341 cgcctggttt gtgttcggat agtctttgat cacctgttga aaaagaagct tggcgctctc
     2401 cttgtctccg agccggagaa aagacagccc ttgcttgagg atggcatggg gaaccctgtc
     2461 cccgttggga tagttcttga tgaccttctc atattcaaga atggccttct cgaagttatt
     2521 ctctaaataa tagcactctc ccaaccagaa ttgggcgttg tccgacaggt ccgaattggg
     2581 ataggcctcg atgtatttct ggaacccttt ccgggcctct tcgtacctgc cttccttgaa
     2641 gtccttgtaa gccgacgaat agagcgtatc ccggccgttc ttgttattcc ggccgtgctg
     2701 cacaccctgc ttgtcgtctc catcggcctt cttgcccacg ccgataaaat tttcgatgaa
     2761 gcttatcctc agggcgaggt cattgagccg cgcgtccctt tccttacggt cggcacgcat
     2821 cgtgtcgatg tcgcgcctca actcctccac cgtgcccctc agtgcctgga tgtcccccct
     2881 tagtgcaatc aagtcggcac ctgtgtccgc ctggactttg cgcagggatt tttccgcctc
     2941 cctgcgctcg tccagttgct tctggagttt caccgtttcc tcttgcagca ccgttatctt
     3001 ctggtcgagg ttctgattca tccgccgcag gtcttggctg gttgcacaac cgccgacaag
     3061 aaaaaacatc acagcaacaa cgggcaagta tcttttcact tttgagccca gtgtcaacgc
     3121 accttacttg acggtcacga cgaaatgagc gcgtctgttc tttgcccagg cttcctcatt
     3181 atgacgggga tcaagaggac gctctttgcc gtagctgatc gtcttgatcc gctccttctc
     3241 cacgccgagg ctcaccaggt atttcatggc actcagggcg cgcttttctc ctagggcgag
     3301 gttatactcg acggttcccc tttcatcgca atgaccctcg atcaggaccg tcgccgtctt
     3361 ttcagccaac agtatgtcgg caagtttctt caacatatcc cgcgcttccg gcttcaggtc
     3421 atacttgtcg aaatcaaaat gtatatcctt gatctcggcg attgctgcgg ccaaggcctc
     3481 tttggccttc tgttcggcga gcatcctctc cttttcctgc tgctccttga gggccgcctc
     3541 cctcagggcc ttctccctgg caagcctctg ctcctccgtc tcggctggag cggcaggagt
     3601 ggcaggagcc gcctctttct gaacggtcgt tgtcttctct tccgccgcct ccttcacgac
     3661 cttctttgca cacccggaaa acatcacgaa accgaacgct agaatcaaaa tactcaaccc
     3721 aataagcacg acatttcttc tcatacaccg tctcctttct attaaggttc acgacatctt
     3781 acaaacaagg tatttcataa cacatcaccg gaaaagttca atataatttt aagtgagggg
     3841 accaggaggc gttcatgtat tcacccatcc cctcgtgaag gatgcggacg ttcgaaccgt
     3901 tggcgttcat gacgcaaagg cggtgccggc cgttcaacct cgacgtatag actaagtacc
     3961 gggagtccgg ggaccaggaa ggggattcgt tgcgtcccga cgaggtcagt tgccggagat
     4021 tgccgccgtc ttcatcgatc acgaagacct ggaatgtacc gcccaccatc ccttcgaagg
     4081 cgatgcgtcc gcccttcggg gcccaggccg gcgatgtgtt atagttgccc tggtaagtca
     4141 gccttcgctg attgctgccg tcagcatcca ttatgtaaat ctggggagag cctcccctgt
     4201 cggagacgaa ggcgatcctc ttgccgtcgg gagaccagac cggggagacg ttgatggagt
     4261 aattgctggt caccctcgtc gccgtcccgt ggttcaggtc catcgtatag atttcatgac
     4321 tccccccttt gctcaaggtc aaaaggattt ttcttccttg
//
LOCUS       DAOTMX010000135         4338 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_16462_length_4338_cov_3.617091, whole genome shotgun sequence.
ACCESSION   DAOTMX010000135 DAOTMX010000000
VERSION     DAOTMX010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4338
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16462_length_4338_cov_3.617091"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="P5573_04940"
     CDS             <1..295
                     /locus_tag="P5573_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRT27437.1"
                     /translation="LRTYSSGMVTRLGFSVVAHLDPEILLVDEVLAVGDINFQKKCMD
                     KIMGFRANGVTMVLVSHSLEQVQRLCDRAMWIDHHEIRAIGEPAAVCQAYQGG"
     gene            367..1614
                     /locus_tag="P5573_04945"
     CDS             367..1614
                     /locus_tag="P5573_04945"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRT27438.1"
                     /translation="MLDQKLPVPTGKRLVLLTGARQTGKTTLAKAKYPAMRYINLDAP
                     ENREAVRTVLSSFWARDVGEAVLDEAQKEPVVFDKVKFAFDEGAIRFSVLLGSSQILL
                     LKKVRETLAGRVSIFELWPLMMEEIFNDSAEAPQVPPLIDRLLAEMSLEDVFGSEPGL
                     LLGREDALRRDAQERLLQWGGMPALLSLPPEERLRWLKDYEYTYLERDVTDLARLDDL
                     MPFRKFQRLAALRSGRLLNYSELARDTGVSVDTARRYLEYLKISYQTFLLPPYHVNLT
                     STAIKTPKVYWLDIGLLRQLTGIPTAVTGEIYETMVVGELVKWIKTTAAEGELYFYRT
                     RSGLEVDIIFESAGGLVAMEVKSRQVIVNKDVAGLREIASVLGDRWRGGLVVYTGNEL
                     KQIASPQIWAVPSHRLFTSASRV"
     gene            1674..3041
                     /locus_tag="P5573_04950"
     CDS             1674..3041
                     /locus_tag="P5573_04950"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRT27439.1"
                     /translation="MKNAFLKKLTLLAGDAVLIVAALYMAPALRFGSFFDLGRFSGPA
                     EAFSVVSYLFFFYVFDLYNLEERFSRGAFVIRFGASVVVASIFIAAMFFLFRVPLYSP
                     GIFFLDGVLIFFFCMAWRLVFEQVRERAGRPLRLAVLGAGEAGQEFYEVVKGKRDYEV
                     VGFLDDDEEKWGGTVGTVPVLGGSDLLPSLVREQQVDRVILAVTHAIPPGVYKKLIEA
                     KFSGVNVYEMPAFYEMVAEKIPVRHISDLRLTHSTMSGARRSVYTRRVKRVVDVSLSL
                     CGLVVSLPVMALLCIAVRLDSPGPALFVQKRIGRQERPFALYKFRTMRYGSENERAYA
                     GHKDDPRITRVGKVLRLFRLDELPQLWNVLKGEMSLIGPRALMEEEVREFNPAVPCFS
                     LRHTVRPGITGWAQVNYRHGATKEDALEKLQYDLYYLKNMSFLLDLDILLRTVRVVLF
                     GKGAR"
     gene            3046..4095
                     /locus_tag="P5573_04955"
     CDS             3046..4095
                     /locus_tag="P5573_04955"
                     /inference="COORDINATES: protein motif:HMM:NF021497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A deacylase LpxR family protein"
                     /protein_id="HRT27440.1"
                     /translation="MTKKRRLILVFIVLFLPSQSFAEGDAARKSDTITLYLENDLFGF
                     DNHDRYYTHGTKISWASRDLTEYRDIVRGPAWMLRLMERLPFVNNAGDVRTVSLSLGQ
                     NMYTPEDKERSDLIPDDRPYAGISYLGFGLHSRSRSRMDTVELDLGIVGRHSYAEDCQ
                     KEVHKWTDSPEPKGWSNQLHDEPVLNVYYERRWRTLQAGGLGGLGFDFIPHAGVAAGN
                     IYTGANMGGQVRFGWNVPSDFGTYLIRPGSGGNAPVDETDPRFSRISRRFGIHLFFAV
                     NGNAVARNIFLDGNTFRDSHSVSKKPFVADIIGGIGMIIHRFKITYSYVHRTKEFDTQ
                     REAQNFGAVSVSFTF"
     gene            complement(4118..>4338)
                     /locus_tag="P5573_04960"
     CDS             complement(4118..>4338)
                     /locus_tag="P5573_04960"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRT27441.1"
                     /translation="EHIDRIFDPYFTTKAESGKKGLGAGLGLSVCRSIIKNHDGYILV
                     ESEPGKGSTFHVFLPSSDEEAKPAVKEK"
BASE COUNT          885 a         1182 c         1329 g          942 t
ORIGIN      
        1 gctcaggacc tactcctcgg ggatggtgac gcggctgggg ttctctgtgg tcgctcatct
       61 cgacccggaa atactcctcg tggatgaggt actcgcggta ggggacataa actttcaaaa
      121 aaagtgcatg gacaagataa tgggtttcag ggcaaacggg gttacgatgg tcctcgtctc
      181 tcacagcctt gaacaggtac agagactctg cgacagggcg atgtggatag accaccatga
      241 gataagggcg ataggggaac cggcggcggt ttgtcaggcc taccagggag gatagaattt
      301 cgttgattat cgcgttaata tgctgtaatt ttaacgacga tgaacgataa taagcagcta
      361 tatcgcatgt tagaccagaa actacccgtc ccaacgggca agcgccttgt tctcttgacg
      421 ggtgcgcgtc aaacaggaaa gacgacgctc gcgaaggcga aatatccggc catgcgttac
      481 atcaacctcg acgccccgga gaatcgagaa gcggttcgca cggtgctgtc gagcttctgg
      541 gcgcgggatg tcggtgaggc cgttttagac gaggcgcaaa aagaaccagt cgtcttcgat
      601 aaggtgaaat tcgccttcga tgaaggggcg atacgttttt ccgtgctgct cggttccagc
      661 cagattctcc ttttaaaaaa ggtcagggaa acgctggcgg ggcgggtttc gattttcgag
      721 ctgtggcccc tgatgatgga agagattttc aatgattcgg cggaagcccc ccaagtaccg
      781 ccgctcatag acaggctact tgccgagatg agtttagaag acgtcttcgg aagcgagccg
      841 ggtctgctgc tgggcagaga agatgccttg cggagggatg cgcaggagcg attgctgcaa
      901 tggggaggga tgccggcatt gttgtccctg cctcctgaag agcgtctccg gtggctgaag
      961 gattatgagt acacgtacct cgagagggac gtgacagacc tcgcccgcct cgatgacctg
     1021 atgccgttca ggaaattcca gcgtctggcg gcgctccggt ccggccgtct attgaactat
     1081 tcggaattgg ccagagacac cggagtaagc gtggacacag cacggcgcta tctcgagtac
     1141 ctgaagattt cttaccagac ctttcttctg cccccttatc acgtcaacct gacaagtacg
     1201 gcaatcaaga cgccgaaggt ctactggctc gacatcggat tgctcagaca attgaccggc
     1261 ataccgacgg cggtgacggg cgagatatat gagacaatgg tggtgggtga gctggtcaag
     1321 tggattaaga cgacggccgc cgagggggaa ttgtattttt accggacccg ctcggggttg
     1381 gaggtggaca taatctttga gtcggcgggc ggtctcgtgg ccatggaggt taaatcccgt
     1441 caagttatcg tgaacaagga cgtagcaggg ttgagagaaa tcgcgtccgt cttaggcgac
     1501 agatggcgcg gcggattggt ggtatacacg ggtaacgaac tcaagcagat tgcctcgccg
     1561 cagatatggg cggtaccctc tcatagattg ttcacatccg cctcaagggt ataaaagggt
     1621 cgaggaaccg caaccggtga aagaggccct cataaaagcg gagagaatct tctttgaaga
     1681 acgccttcct caaaaagctg accctcctgg cgggcgacgc ggtgctgatc gtggcggccc
     1741 tgtatatggc ccctgccctc cggttcgggt ccttcttcga tttgggtaga ttctccggcc
     1801 cggcggaggc cttctccgta gtcagttatc tctttttctt ctacgtcttc gacctctaca
     1861 acctggagga acggttcagc cgtggcgcct tcgtcatccg gttcggcgct tccgtcgtgg
     1921 tcgcgagcat cttcatcgcg gcgatgtttt ttctcttccg ggttcctctc tacagtccgg
     1981 gcatcttttt cctcgacggc gtgctcatct ttttcttctg tatggcctgg cggctcgtgt
     2041 tcgagcaggt ccgggagagg gcggggcggc cgctgcggct ggccgtcctt ggcgccgggg
     2101 aagcagggca ggagttctac gaagtggtga aggggaaaag agactacgaa gtcgtcggtt
     2161 ttctggacga cgacgaggag aaatggggag gcaccgtggg gaccgtgccg gtccttggtg
     2221 ggagcgacct cttgccgtcc ctcgtccgtg aacagcaggt cgacagggtc atcctggcgg
     2281 tcacccacgc catcccgccg ggggtgtaca agaagctcat cgaggcgaag ttctccggcg
     2341 tcaatgtcta tgagatgccc gcgttctacg agatggtggc ggagaagatc ccggtccgtc
     2401 acataagcga cctccggctt acgcactcga ccatgtcagg tgcgcggcgg agcgtttaca
     2461 cgcgcagggt gaagcgggtt gtcgatgtct ccctctctct ctgcggcctg gttgtatcgc
     2521 tgcccgtgat ggccctcctc tgcatagcag tccgacttga ctcaccgggc cccgcgttgt
     2581 tcgtccagaa gcgcatcggc cggcaggaaa ggcctttcgc gttgtacaaa ttccggacca
     2641 tgcggtatgg ttcggagaac gagcgcgcgt acgccgggca taaagacgac cctcggatca
     2701 cgcgagtggg gaaggtgctg aggttgttcc ggctcgatga actgccccag ctctggaatg
     2761 tactgaaggg agagatgagc ttgatcgggc cacgggcgct catggaggag gaggtgcggg
     2821 aattcaaccc cgccgttccc tgcttcagcc tgcgacacac cgtccggccg gggatcacag
     2881 ggtgggcgca ggtgaactac cgccacggcg ccacaaagga agacgccctg gagaagctcc
     2941 agtatgacct gtactacctg aagaacatgt cctttctgct cgacctcgac atcttgcttc
     3001 ggacggtgcg ggtggtgctc ttcgggaagg gggcacgttg aaaaagtgac gaagaaacgg
     3061 cgccttatcc ttgtctttat cgttcttttt ctcccctcgc agtcctttgc agaaggcgac
     3121 gcggcgcgga agtcggacac catcaccctc tacctggaaa acgacctctt cggtttcgac
     3181 aaccacgacc gctactatac ccatgggacg aagatctcct gggcctcccg agacctcaca
     3241 gagtaccggg acatcgttcg tgggccggcg tggatgttgc ggctgatgga gaggctgccc
     3301 ttcgtcaaca atgcgggaga cgtgcgcacg gtttccttat cccttggtca gaacatgtac
     3361 actccggagg acaaggagcg gagcgacctg atcccggacg accgtcctta tgcggggatt
     3421 tcctacttgg ggttcgggct tcatagcagg agccggtctc ggatggatac cgtcgaattg
     3481 gacctcggca tcgttggccg tcactcctac gcagaggact gccagaagga ggttcacaag
     3541 tggacggatt cgcccgagcc gaaggggtgg tctaatcaac tccacgacga gcccgtgctg
     3601 aacgtctact atgagcgcag atggaggacg ttgcaggccg gcggccttgg tgggttgggg
     3661 ttcgacttca ttccccacgc gggcgttgcc gcggggaata tttacacggg ggcgaatatg
     3721 ggcggtcagg tccgcttcgg ctggaacgtc ccgagtgatt ttgggacgta tctgatacgg
     3781 ccggggtcgg gcggcaacgc ccccgtggac gaaaccgacc cccggttttc acggatttcg
     3841 cgccgtttcg gcatccatct cttcttcgcc gtcaacggaa acgccgtggc caggaacatc
     3901 ttcctcgacg ggaatacctt ccgggacagc cacagcgtaa gcaagaaacc cttcgtcgcc
     3961 gacatcatcg gcggcatcgg gatgatcatc caccggttca agataaccta ttcctacgtt
     4021 caccggacaa aggaattcga cacccaaagg gaggcgcaga atttcggggc cgtctccgtc
     4081 tcgttcacct tctgacccga ctcctcaaaa ccctttctta tttctccttc accgcgggtt
     4141 ttgcttcctc gtccgaggaa ggcagaaaga cgtggaaggt cgaccccttc cccggttccg
     4201 actccaccag gatatagccg tcgtggtttt tgatgatgga acgacacacg gaaagaccga
     4261 gccctgcccc taggcctttc ttgccggatt ccgctttcgt cgtaaaatag gggtcgaata
     4321 tcctgtcgat gtgctctt
//
LOCUS       DAOTMX010000136         4336 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_16476_length_4336_cov_3.337071, whole genome shotgun sequence.
ACCESSION   DAOTMX010000136 DAOTMX010000000
VERSION     DAOTMX010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4336
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16476_length_4336_cov_3.337071"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(184..648)
                     /locus_tag="P5573_04965"
     CDS             complement(184..648)
                     /locus_tag="P5573_04965"
                     /inference="COORDINATES: protein motif:HMM:NF013920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HRT27442.1"
                     /translation="MAKEYRTIRNDVALPYQWALGKTWVGFFDGLKEEKIRGTKCAGC
                     GKVYVPARSFCPECYTDMTEWVDVKPEGKIISWTLVKAKYYGQVRTPPYVIALIKLDG
                     ADCGFIHFVDGFDVSDVRQLKRKLKTGTKVKAVWRSEKKSDIHDISHFALIG"
     gene            complement(662..1129)
                     /locus_tag="P5573_04970"
     CDS             complement(662..1129)
                     /locus_tag="P5573_04970"
                     /inference="COORDINATES: protein motif:HMM:NF013920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HRT27443.1"
                     /translation="MSKEEYRIVKGQVMVPYELALGRTWSTFYDCLKEEKIMGKKCGK
                     CSRVLVPPRSFCPRCFEDMDEWVEVSQEGIIETWVYVNMPFFAQELKIPFISAQIRLD
                     GSDTGFTHQIAGIDLSDFDKLREIVKLGGRVKAVWSKEKKGNILDIAYFEPVK"
     gene            complement(1144..2310)
                     /locus_tag="P5573_04975"
     CDS             complement(1144..2310)
                     /locus_tag="P5573_04975"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27444.1"
                     /translation="MGKAIAIIGTGQTRHGRRSDVCYPDLVREAVTAALEDAGITLKD
                     VDGVVLGTMPSMMEGVSSTHFYFAEAMGSIGKPVMKVETCGSTGQSIAHCGFYWVASG
                     MADIVLVVGSEKMSEGDGQATMTTVFDPFFQRTFAAGAPGVFTMQSMEWMYRFKIPEE
                     KGRDAAALISVSNHDNAMDNPYAHIRTRVTMDDVKNARVITYPVRLLDVCPASDGACA
                     VIFATREKAKKICKKPAWIKGLGFRGEEFFYGESDKSIWMSATEAAKEAYRHAGIRNP
                     LKELDVAEVYNPFTYQEMIFYECFGFCPRGEAPDYVLKGTFMKDGKLPCCPSGGTLCT
                     NPIGATGLVRLAEAALQVTGKAGERQVEGARLALSHAMGGVDQFNAVTIVGSKL"
     gene            complement(2327..3463)
                     /locus_tag="P5573_04980"
     CDS             complement(2327..3463)
                     /locus_tag="P5573_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HRT27445.1"
                     /translation="MRKVAITHVAQSSGVESRESLMDFTYRVNKEILDKAGLKREDVG
                     CIIAGAADIFHSGLSCANAFDWDGIGAFLKEGSRAEESAMSFIYGCMRIMSGYYDTVL
                     VTALVKGSENPDNDPITSFYGDPFYLRPLGVNESSVAALQMRQYMERYRVTEEQCAKV
                     VVKNLGNALRNPCADRKRRISIEDVMRSERVYDPLKALECAPKSDGVVAVLLASESRA
                     KKLTKKPVWLAGYGCSMDHFNPGDRNLLNGHLRIAAEKAYRAAGIRDPRKRIHVAEVC
                     EPYAFQELLWCEQLGFCNEGKGGHMIDSGMTEMDGELPVNPSGGVLATNPYPARGLYR
                     IAEAALQVKGEAGEHQVGRKVKTALAHSTHGFGGQCHSVVILSA"
     gene            complement(3525..3986)
                     /locus_tag="P5573_04985"
     CDS             complement(3525..3986)
                     /locus_tag="P5573_04985"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HRT27446.1"
                     /translation="MAKEYEYLDDYAVKERFISPARTITEADIINFAGITGDWHPLHT
                     DVEYAAKTPFKERIAHGMLTLSIGMVLPFRLGPYSSFLPRSFIAFYGMDNVRFTAPTK
                     IGDTIHCEVEVTEITDKGKGRGVLTTQNQIKNQRGEVLVSCVIKVLCGKRP"
     gene            complement(4030..>4336)
                     /locus_tag="P5573_04990"
     CDS             complement(4030..>4336)
                     /locus_tag="P5573_04990"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRT27447.1"
                     /translation="ADMYTFIEAGRTLCYRAAVFADKAKGGGKGTEVHKLSSASILFN
                     AEMAARIADQAVQIHGGYGYMLEYPVQRFLRDAKLLEIGAGTSEIRRLIIAREILSM"
BASE COUNT          834 a         1335 c         1136 g         1031 t
ORIGIN      
        1 agcgtcctta cttcatccat tgaaaggctt cctctcgaac atttattcgc agtcgactcc
       61 tttcagtctt caggaagtca gaggtcacat ccagaatcat tttttaatca accgtcaaat
      121 tgcggacacc cttctaaacg ccctcgcgtc ccgcgatcct tcattaatcc ggtttgcgcg
      181 cggtcatcca atcagggcaa aatgcgaaat gtcgtggatg tccgactttt tctctgatct
      241 ccagacggcc ttgacctttg ttccggtttt caatttgcgt ttcaactgcc tgacgtccga
      301 aacatcgaag ccatcgacaa aatgaatgaa tccgcagtcc gcgccgtcca gcttaatcag
      361 ggcaatcaca taaggggggg ttctgacctg gccgtagtac ttcgccttca cgagcgtcca
      421 ggaaatgatc ttcccctccg gcttgacgtc gacccattct gtcatgtccg tgtaacactc
      481 ggggcagaag gacctcgccg ggacataaac cttcccgcag ccggcacatt tcgttccccg
      541 tattttttct tctttcaatc cgtcgaaaaa cccgacccat gttttgccaa gcgcccactg
      601 atagggcagg gcaacgtcgt tccggatggt tctgtattct tttgccatag tgatcacctc
      661 ctcacttcac aggctcaaaa taggcgatgt cgagaatgtt cccctttttc tccttgctcc
      721 agaccgcctt cacccgtccg ccaagcttca cgatctcccg gagcttgtca aagtcggaga
      781 ggtcaatccc ggcgatctgg tgagtaaagc cggtgtcgct tccgtccagg cggatctgcg
      841 cggagatgaa gggtatcttg agttcctggg cgaaaaaggg catgttgacg tagacccagg
      901 tctcaatgat cccctcctgg ctcacctcga cccattcgtc catatcctcg aagcacctgg
      961 gacagaagct ccggggggga accagcaccc tcgagcattt cccgcatttc ttgcccatga
     1021 tcttctcttc tttcagacaa tcgtaaaaag tgctccaggt ccttcccagg gccagttcgt
     1081 aaggaaccat gacctgccct ttgacgatcc ggtattcttc ttttgacatg tctcatcctc
     1141 ctctcacagc ttcgatccga caatagtcac ggcattgaac tggtccaccc cccccatggc
     1201 gtgggagagg gccagtctcg cgccctccac ctgccgctcc cccgccttgc ccgtcacctg
     1261 cagggccgcc tccgcgaggc ggacgagccc cgtggcgccg atggggttgg tgcagagcgt
     1321 gccgcccgag ggacagcagg gaagctttcc gtctttcatg aaggtgccct tcaaaacgta
     1381 gtcgggtgct tcaccccggg ggcagaaccc gaagcactcg tagaagatca tttcctggta
     1441 agtgaagggg ttgtacacct cggccacatc caactccttc agaggatttc tgatgcccgc
     1501 atgccgatag gcctctttcg cggcttcagt cgcgctcatc cagatggatt tgtcgctctc
     1561 gccgtaaaag aactcttccc cccggaaacc gagccccttg atccaggccg gctttttaca
     1621 aattttcttc gccttttccc gggtcgcgaa gatgacggcg caggcgccgt ccgaagccgg
     1681 gcagacatcg aggaggcgga ccggataggt gatgacacgg gcattcttga catcgtccat
     1741 ggtcacccgc gtccggatgt gggcgtacgg attgtccatg gcgttgtcgt ggttgctgac
     1801 ggatatcagg gccgccgcgt ctctcccttt ctcttccgga atcttgaaac gatacatcca
     1861 ctccatgctc tgcatggtga agacgcccgg ggcacccgcg gcaaaggtcc tctggaagaa
     1921 gggatcgaag accgtcgtca tcgttgcctg cccgtcgccc tccgacatct tttcggatcc
     1981 cacgaccaga acaatatcgg ccataccgga ggccacccag tagaaaccgc agtgagcgat
     2041 ggactgaccg gtgcttccgc aggtctccac cttcattacc ggttttccga tggaccccat
     2101 ggcttcggca aaatagaagt gggtcgatga aaccccctcc atcatggagg gcatggttcc
     2161 caggaccacg ccgtcgacat ccttgagtgt aatccccgcg tcttcgaggg ctgccgtgac
     2221 ggcctcccgg accaggtcgg gataacacac atcagaccgc cttccatgtc tggtctggcc
     2281 tgttcctata atcgcaatcg cttttcccat gtcgatgacc tcctgctcaa gctgataaga
     2341 tgacaacgga gtggcactga cccccgaagc cgtgtgtgct gtgggctaat gccgttttga
     2401 cttttctccc gacctggtgc tcacccgctt cccccttcac ctgaagggcg gcctcggcga
     2461 tccggtaaag ccctctggcc gggtaggggt ttgtggcgag cacccccccg gagggattga
     2521 cgggcaactc tccgtccatc tccgtcatgc cgctgtcgat catatgaccc ccttttcctt
     2581 cgttgcagaa accgagctgt tcgcaccaga ggagttcctg gaaggcatag ggttcacaaa
     2641 cctccgccac gtgaatccgc tttctcgggt ctcggattcc ggccgccctg taggccttct
     2701 ccgccgcgat tctgagatgg ccgttcagca ggtttctgtc gccgggattg aagtggtcca
     2761 tggaacaccc ataaccggcc agccagacgg gtttttttgt cagttttttc gccctgctct
     2821 ccgatgccag aagaacagcg acgacaccat ccgatttcgg agcgcactcc agcgccttca
     2881 aggggtcgta gacgcgttca gatctcatca catcctcgat gctgatcctt ctcttccggt
     2941 cggcacaggg attgcggagg gcgtttccca ggtttttcac aaccactttc gcgcactgct
     3001 cttccgtcac cctgtaacgc tccatgtact gcctcatctg caaggccgcc accgaacttt
     3061 cgttgacgcc cagaggacgc agatagaagg gatcaccgta gaaactcgtg atgggatcat
     3121 tgtccgggtt ctcggaccct ttcacgaggg ctgtcacgag taccgtgtcg taatagccgg
     3181 acatgatccg catgcaaccg taaataaatg acatcgcgga ctcctccgcc cgggaacctt
     3241 cctttaaaaa ggccccgatg ccgtcccagt cgaaggcgtt tgcgcaggaa aggccgctgt
     3301 gaaagatgtc cgccgcgccg gcaatgatgc agccgacgtc ctcccgcttg aggccggcct
     3361 tgtcgagaat ctccttgttg acccggtagg tgaaatccat caggctttcc ctggattcca
     3421 cgccggaact ctgtgccaca tgggtgatgg caactttccg catcgttttc acctcccagt
     3481 tttgttgtcc gatcttcgga tgcctcgttg aacgttccgt aaactcaggg tctcttaccg
     3541 cacaggacct tgatcacgca ggaaacgaga acctcgcccc tctggttctt gatctggttt
     3601 tgcgtcgtca ggacccctcg gcctttcccc ttgtccgtta tttccgtcac ttccacctca
     3661 cagtggatgg tgtctccgat tttcgtcggc gccgtgaagc gaacattgtc catgccgtag
     3721 aaggcgatga aggatcttgg caggaagctc gagtaggggc cgagcctgaa gggcaggacc
     3781 atgccgatgc tgagggtcag catgccgtgg gcgattcttt ccttgaaagg cgtcttcgcc
     3841 gcgtattcga catcggtgtg aagagggtgc cagtcgccgg tgatgccggc aaagttgatg
     3901 atgtcggcct ccgtgatcgt tctggccggt gatatgaaac gttccttgac ggcgtaatca
     3961 tccaggtatt cgtattcttt cgccatgatc ccctcctttt taaaaagcgt caggcggcat
     4021 tttaatgagt cacatgctca ggatctcccg ggcgatgatc agccgccgaa tctcggaagt
     4081 gcccgcgccg atttcgagca gtttggcgtc tctcaggaag cgctggacag ggtattcgag
     4141 catgtagccg tagccgccgt gtatctgaac ggcctggtcg gcgatacggg cggccatctc
     4201 ggcattgaac aggatcgacg ctgatgaaag cttgtgcacc tccgttcctt ttccgccccc
     4261 tttcgccttg tcggcgaaga cggcggctcg gtagcagagc gttcgccctg cctcgatgaa
     4321 cgtgtacatg tcggcg
//
LOCUS       DAOTMX010000137         4331 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_16491_length_4331_cov_3.804256, whole genome shotgun sequence.
ACCESSION   DAOTMX010000137 DAOTMX010000000
VERSION     DAOTMX010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4331
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16491_length_4331_cov_3.804256"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="P5573_04995"
     CDS             <1..191
                     /locus_tag="P5573_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRT27448.1"
                     /translation="YIACGISGAIQHLAGMSSSKYIVAINKDPEAPIFQKADYGIVDD
                     LFKVVPEVTKEVKKLLAE"
     gene            254..>1832
                     /locus_tag="P5573_05000"
     CDS             254..>1832
                     /locus_tag="P5573_05000"
                     /inference="COORDINATES: protein motif:HMM:NF014695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HRT27449.1"
                     /translation="MENLVFGPGNEGREVFWNTQSFQIPLDLFALVAIAIMAYGLYKR
                     WQMWTAMGKPEQRTDNRGERIKILLKNSVLQASVWRDFYPGLIHGLIFFAFFVLIWGA
                     AFDAFQFYSGVHFNGIPYLILSFIFDLFGLFAIIGVLMAIDRRYIKKPERLGYKGKID
                     NRPEDAIVLLLILGIMITGFLIEGMRIAYFEYPTFEMWSFVGYMTAQLFQGVHPDTLR
                     GAHKAMWWIHTFFALGFIAYIPYSRLLHIVTAPANQFMKNLKPAGSIEPIRDFETAES
                     FGVGKLEEFTWKQIFDSDACTRCGRCQDGCPAYNTGKHLSPKKLIQDLKDHWLERAQL
                     YVEAKKAAGQGGEAPAVPESEKNLINDCVGVAEIWDCTNCMHCMEHCPVFIEHVPKII
                     DMRRYKVLMEADFAPELQLSYRNMENNSNPWGIGAHLRADWAKDLDIKTMAEDPNAEY
                     LFYVGCAGSFDDRGKKITVAFAKILKEAGVSFAILGTEEGCCGDSAMRGGNEYLYQAL
                     AQYNIEIMNGYGVKKIIC"
     assembly_gap    1833..1932
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1933..2487
                     /locus_tag="P5573_05005"
     CDS             <1933..2487
                     /locus_tag="P5573_05005"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HRT27450.1"
                     /translation="AQYNIEIMNGYGVKKIICTCPHGYNALKKDYPLLGGNYEVYHHT
                     EILADLLAKGKIKLNKSLEGVFTLHDSCFLGRYNNIYQQPRNILGAVPGMKLVEMEKN
                     LDRSFCCGAGGARMWMEEHGEKIAYARTDMAIAVKADKIATCCPFCMTMVTDGLKDRG
                     KEEAMAALDVAEVVWQAMGVEEKK"
     gene            2642..>4331
                     /locus_tag="P5573_05010"
     CDS             2642..>4331
                     /locus_tag="P5573_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HRT27451.1"
                     /translation="MAEESVYLQKPWLRSYEEGVPEFIDYEEVTMPEILDRTASRFPD
                     RKALIFEGFSLTFRELKEMVDRFAACLSDFGVRQGDAVAILLPNLIPCVAAYYAAMKV
                     GAVTVMNNPLYSDRELEHQFNDSGSKVLITLDLLGNRMIDLRPKTKIKQIVCTSIGDY
                     LPFPKNILFPLVAKKKKMAADVKTAPDVYRWKECLAKYAPNPPKVKIDFEDVAMYQYT
                     GGTTGVSKGVMLTHANLSKQVQQIDAWFPKLKKGEELMLGALPFFHVFGLSTAMNLSI
                     HMGWGDVLVPRPQPGPLLEAIRKYRPTFAPLVPTMYIGMLNHPDVKKTDMTCLKGCFS
                     GSAPLPVEVIRDFEAATGAVIVEGFGLTETTPVTHVNPFAGGARKVGSIGVPISDTLA
                     RIVDLETGLEDMPVGEAGELIIKGPQVMKGYLNMPEETAHVLRDGWCYTGDIGKMDED
                     GYFYIVDRKKDMIISGGYNVYPRDIDEVFYENPKVQEACTIGIPDPKRGENIKVFVVL
                     KEGETATAEEMIAYCKKYLAVYKLPTEVEFRKELPKSTVGKVMRKQLREEELAKR"
BASE COUNT         1014 a         1123 c         1246 g          848 t
ORIGIN      
        1 tttacatcgc ctgcggcatt tccggtgcga ttcagcatct tgcggggatg agctcgtcga
       61 agtacatcgt ggcgatcaac aaggacccgg aggcgcctat cttccaaaaa gccgactacg
      121 gcatcgtgga tgacctgttc aaggtagttc ccgaggtcac caaagaagtg aaaaaattgc
      181 ttgcagagta agatacggga ggggggcgga ggtttcgccc ccctttttcg ggataattag
      241 gaaggaataa gctatggaaa atttggtttt tggcccggga aacgaaggga gggaagtctt
      301 ctggaatacc caaagcttcc agatccctct tgacttgttc gctttggtag ccattgcgat
      361 catggcatac ggtctctaca agcgctggca gatgtggacg gcgatgggaa agccggaaca
      421 gaggacggac aacaggggag agaggatcaa gatcctgctt aaaaacagcg tccttcaggc
      481 gtcggtgtgg cgggattttt atccgggact tattcatgga ctgattttct tcgccttttt
      541 cgtcctcatc tggggcgccg ccttcgacgc cttccagttc tattcgggcg tccacttcaa
      601 cggcataccg tatctcatct tgtcgttcat tttcgacctc ttcggcctct ttgcgatcat
      661 cggcgtcctc atggcgatcg acaggaggta cataaaaaag cccgaaaggc tcggctacaa
      721 gggtaagata gacaaccgtc cggaggatgc gatcgtcctc ttgctgatcc tgggcatcat
      781 gataacgggt ttcttgatcg agggtatgag gattgcctac ttcgagtacc cgaccttcga
      841 gatgtggtct ttcgtgggat acatgacggc gcagcttttc cagggggtgc acccggatac
      901 attgaggggg gcccataagg cgatgtggtg gatccacacg ttcttcgccc tcggtttcat
      961 cgcctacatc ccttactccc gcctgctcca catcgtgacc gcgccggcca accagttcat
     1021 gaagaacctg aagccggcag gctcgataga gccgatcagg gacttcgaga cagcggagag
     1081 cttcggagtc gggaagctcg aggagttcac ctggaaacag atattcgatt ccgatgcctg
     1141 cacgcggtgc gggcgctgcc aggacggctg ccccgcctac aacacgggga agcatctttc
     1201 cccgaagaag ctcatccaag acctgaagga ccattggctc gaaagggcac agctctatgt
     1261 cgaggctaag aaggccgccg gccagggcgg agaggcgccc gccgttcccg agtccgaaaa
     1321 gaacctcatc aacgattgcg tcggtgtggc cgagatatgg gattgcacca actgcatgca
     1381 ctgcatggag cactgccctg tcttcatcga gcacgtcccg aagatcatcg acatgaggcg
     1441 ttacaaggtc ctgatggagg cggattttgc gccggagtta caactttcgt acaggaacat
     1501 ggaaaacaat tccaacccct ggggtatagg cgctcacctg agggccgact gggccaaaga
     1561 cctggacatc aagaccatgg cggaagaccc gaatgcagag tacctgtttt acgtgggatg
     1621 cgccggttca ttcgatgacc ggggcaagaa gataacagtg gccttcgcga agatactgaa
     1681 agaagccggc gtgagctttg ccatcctggg gacggaggag ggttgctgcg gagattcggc
     1741 gatgaggggt gggaacgaat acctctacca ggcgctcgcc cagtacaaca tcgagatcat
     1801 gaacggctac ggagtcaaga agataatctg tannnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nngcccagta caacatcgag atcatgaacg gctacggagt caagaagata
     1981 atctgtactt gcccgcacgg ttacaacgcc ctgaagaagg actaccccct gctaggcggt
     2041 aactacgagg tctatcatca tacggagatc ctggcggacc tgttggcgaa gggcaagata
     2101 aaactgaaca aatccctgga gggggtgttc acgcttcacg attcctgctt cctcggccgt
     2161 tacaacaaca tctaccagca gccgcgcaac atcctcggcg ccgttcccgg tatgaagctc
     2221 gtcgagatgg aaaagaatct cgacaggagc ttctgctgcg gtgcgggtgg tgcgaggatg
     2281 tggatggaag agcacggcga gaaaatcgcc tacgcccgga cggacatggc catagccgtg
     2341 aaggccgaca agatagccac gtgctgtccc ttctgcatga caatggtcac cgacggcctg
     2401 aaagacaggg gaaaagaaga ggcgatggcc gccctggatg tcgcagaagt cgtgtggcag
     2461 gccatgggtg tggaagagaa gaaatagccg gaggaaaacc ggtcgcacaa gccccctgtc
     2521 ccacaggacc gggggctttt tttgtggcaa ttgtgccgtc attttgctat taaattgcgg
     2581 acgacttccg ttatttgtcc ttcacattta acccgcaaca aaaaaagggg gggatagaga
     2641 catggccgag gaatccgttt acctgcagaa accctggttg aggtcctacg aagaaggtgt
     2701 gcccgaattc atcgactatg aagaggtcac tatgcccgag atcctggaca ggacagcatc
     2761 ccgctttccc gaccgcaagg cactcatttt cgagggattt tcactgactt tcagggaact
     2821 gaaagaaatg gtggaccgct tcgccgcctg cctttccgac ttcggcgtcc gtcagggtga
     2881 cgccgtcgcg atcctcctgc cgaacctcat cccctgcgtg gccgcttact acgcggcgat
     2941 gaaggtcggc gccgtaacgg tcatgaacaa cccgctttat tccgaccggg aactggaaca
     3001 tcagttcaac gattccggct cgaaggttct gattacgctg gaccttctcg ggaaccgcat
     3061 gatagaccta cggcctaaga cgaagatcaa gcagatcgtc tgtacgtcca tcggcgatta
     3121 tctccccttc ccgaagaata tcctcttccc gctggtggca aagaagaaaa agatggcggc
     3181 cgacgtgaag acggcccccg atgtctaccg atggaaagag tgcttggcaa agtacgcgcc
     3241 gaatccgccg aaggtaaaaa tcgacttcga ggatgtagcc atgtaccagt acacgggcgg
     3301 gaccacaggc gtttctaaag gggtgatgct gacgcacgcg aacctcagca aacaggtcca
     3361 acagatcgat gcctggttcc ccaaactgaa aaaaggggag gaattgatgc tgggagcgct
     3421 ccccttcttc cacgttttcg gtctctccac ggcgatgaac ctttcgatcc acatgggttg
     3481 gggggacgtc ctggtgcccc gtcctcagcc ggggccgctt ctggaggcta tacgaaaata
     3541 caggcccacc ttcgccccgc ttgtaccgac gatgtacatc gggatgctca accaccctga
     3601 cgtcaagaag acggacatga cgtgcctcaa ggggtgtttt tccggcagcg cccccctgcc
     3661 ggtcgaggtc atccgagact ttgaggccgc aaccggtgcc gtcatcgtcg aggggttcgg
     3721 cctcacagag acgacgccgg tcacccacgt taaccccttt gcgggcggag cccggaaggt
     3781 gggcagtatc ggtgtgccga tttccgacac cctggcccgt atcgtggacc tggaaactgg
     3841 cctcgaggat atgccagtcg gcgaagcggg cgagctcata atcaagggcc cccaggtgat
     3901 gaagggctac ctgaacatgc cggaagagac ggcgcatgtg ctgagggacg gctggtgtta
     3961 cacgggcgac atcggcaaga tggacgagga cggctatttc tacatagtcg accgtaagaa
     4021 agatatgata atctccgggg gatacaacgt atacccgagg gacatagacg aggttttcta
     4081 cgagaacccg aaggtccaag aggcctgcac gatcggcatt cccgacccga aacgggggga
     4141 gaacatcaag gtcttcgtcg tcctgaagga aggtgaaacg gctacggcgg aggagatgat
     4201 tgcatactgc aagaaatacc ttgccgtata taagctcccg acggaggtcg agttccgcaa
     4261 ggaattgccg aagtccacgg tggggaaggt catgagaaaa cagttacgcg aggaagaact
     4321 ggcgaagaga a
//
LOCUS       DAOTMX010000138         4322 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_16537_length_4322_cov_3.906726, whole genome shotgun sequence.
ACCESSION   DAOTMX010000138 DAOTMX010000000
VERSION     DAOTMX010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4322
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16537_length_4322_cov_3.906726"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="P5573_05015"
     CDS             complement(<1..567)
                     /locus_tag="P5573_05015"
                     /inference="COORDINATES: protein motif:HMM:NF040068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27452.1"
                     /translation="MKDDRPISDRLEDILPFVERPGRYLGGEVNSRTGDRTACRLSFA
                     LCFPDVYEVAMSHLGIQILYAVLNDHRGVCAERFFAPWPDMEEAMRGKGIPLCSLESR
                     TPLSSFDVIGFSLQYELAYTNVLNMLDLGGIPLYARDRKEGCPLVIAGGPCAFNPLPV
                     APFFDAFVIGEGEEVVTEVADALMKAGEK"
     gene            complement(557..1492)
                     /gene="mdh"
                     /locus_tag="P5573_05020"
     CDS             complement(557..1492)
                     /gene="mdh"
                     /locus_tag="P5573_05020"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF004863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HRT27453.1"
                     /translation="MNNKVSVIGAGFVGTMTAQRIVEKDLADVVLVDIVEGVPQGKAL
                     DIAQSAPIEGFTAKVVGTNDFSLIRDSRVVVVTAGFPRAPGMTREELALKNGGVVKSV
                     AEKIRVFAPGAIILMVTNPLDLMAHLAFKVSGFPAGRVIGMGGVLDSARYRYLIAEEL
                     DVPTGDVQALVLGSHGDAMIPLERYAAVGGIPVADLMKKDKLDRIIERTRNSGAEIVG
                     LLKTGSAFHAPSAAVVYMLEAILLGSPRIMPASVYLDGQYGIRDVFLGVPVRLGPSGV
                     EEILTVDLSEGELSALKRSAEEAKKAFKNLTSYER"
     gene            complement(1583..1975)
                     /locus_tag="P5573_05025"
     CDS             complement(1583..1975)
                     /locus_tag="P5573_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27454.1"
                     /translation="MKMVEEIQLPNGLVAEVWDRSRAIAADTTKVALLVRVKVIFKEE
                     YFSNEEQLRLATKVFGPDVYYELKKERTFVPNREVGHVFRTFMEEFKRDALPYISKEK
                     FPAGFARSKYLDIQKHPYKYGLPVGKGS"
     gene            2140..3009
                     /gene="purU"
                     /locus_tag="P5573_05030"
     CDS             2140..3009
                     /gene="purU"
                     /locus_tag="P5573_05030"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF004684.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HRT27455.1"
                     /translation="MSDRLSEANAILLLSCPDQRGIVASVSNFVFQHNGNIVHAAQHT
                     TQKSKIFFMRIEWELEGFDIRREDIGKAFSPIADRFGMTWWLHFTDYVPRVAIFVSRH
                     VYCFHDLILRQNMGEIKCEIPIVISNHMDLKPLSEHFGLNYRYYPVTPENKEAQEERE
                     IEELRQHRVDLIVLARYMQILTGRFVDLYRDRIINIHHSFLPAFAGGKPYEQAYDRGV
                     KIIGATSHYVTESLDDGPIIVQDVIRISHRDSVEDMKMKGKDLERTVLSRAVKLHLEH
                     RVIVHGSKTIVFE"
     gene            complement(3026..3703)
                     /locus_tag="P5573_05035"
     CDS             complement(3026..3703)
                     /locus_tag="P5573_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27456.1"
                     /translation="MKRRNFRVIFAIFLSSSLLLLSPAVCPAQEAGKATEKVKPGSSC
                     EEDLWEADRRCLSGRCEDAVYLYDRFLEKDGACHGDLRGEAFFRRGRAKETLNDFRGA
                     VSDYDLALQAKPDSTKFREALGRGYYKLANQKERLGDFRGALADYLKALELVPGFPEA
                     ADGVAGIYLERGNKKQERGELKSAMEDYEKALQYSPNHPAVREAKKNLDERLKYKAAE
                     RDAMFTF"
     gene            3790..>4322
                     /locus_tag="P5573_05040"
     CDS             3790..>4322
                     /locus_tag="P5573_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27457.1"
                     /translation="MTLRLLDPVREILKVVEEMTDRKVDFVEKTDLPVSAELKVAEKA
                     EQAHRLFYRPTHTDEINYLVAHQCGHVLRLFGAEKGKRFIPVANRKTMMSYLLEMDDD
                     LKRLSMVFGPEKVKKLVFLWYEGVVFQVTRMPPDIMIDKWLYDEYPELRAIQLASLER
                     QRRAAILGLSEDMRKITP"
BASE COUNT          915 a         1264 c         1201 g          942 t
ORIGIN      
        1 cttttccccc gctttcatca aggcgtcggc cacctccgtc acgacctcct ctccctcgcc
       61 gatgacgaag gcgtcgaaaa aaggcgctac gggcaatggg ttgaaggcgc aaggacctcc
      121 cgctataacc agggggcacc cttccttgcg gtctcgggca taaaggggga tgccgccgag
      181 gtcgagcatg ttcaggacat tcgtgtaggc caattcatac tggagggaga agcctatgac
      241 atcgaaagac gaaaggggcg tccggctctc cagcgagcag aggggaattc ctttcccgcg
      301 catggcctct tccatgtccg gccagggcgc gaagaagcgc tcggcgcata cccctcgatg
      361 gtcgttcagg acggcgtaaa gtatctggat ccccaggtgg gacatggcga cttcatagac
      421 gtcggggaag cagagggcga agctcagtct gcaggcggtc ctgtccccgg tgcgggaatt
      481 cacttcgccg ccgaggtagc gccccggtct ttcgacgaaa ggcaggatgt cttccagtct
      541 gtcgctgatg ggtctgtcat ctttcatagg acgtcagatt cttgaatgct ttcttcgcct
      601 cctccgccga gcgcttcaag gcgctcaatt cgccctccga gaggtccacc gtcagtatct
      661 cctcgacgcc cgaagggccg aggcggacag gcaccccgag gaacacgtcc ctgatcccat
      721 actgtccgtc caggtagacc gaggcgggca tgattcgggg tgatccgaga agtattgcct
      781 cgagcatgta gacgacagcc gccgaggggg catggaaagc gctgcccgtc ttcaggaggc
      841 cgacgatttc cgcaccgctg tttctggttc tttcgatgat ccggtcgagc ttatcctttt
      901 tcatcaagtc cgctaccggg atgcccccga ccgccgcgta acgttccagg gggatcatgg
      961 catccccgtg gctccccagg acgagggcct gaacgtctcc ggtagggaca tcgagctcct
     1021 cggcaatgag gtagcggtag cgcgccgagt cgagaacccc acccatcccg atgacccgtc
     1081 cggccgggaa gccggagacc ttgaaggcaa ggtgggccat gaggtccaag gggttggtca
     1141 ccatgaggat gatcgccccg ggggcgaaca cccggatttt ctcggccact gacttcacga
     1201 cacccccgtt cttcaaggcc agctcttcgc gggtcatacc cggcgcccgg ggaaaaccgg
     1261 cagtcacgac gacgacacgg gaatcccgga tcagagagaa gtcgttggtt ccgacgacct
     1321 ttgccgtgaa tccttcgatg ggggccgact gggcgatgtc gagggccttt ccttgaggga
     1381 cgccctcgac gatatccaca aggaccacgt cggcgaggtc cttctcgacg attctctgcg
     1441 cggtcatggt gcccacgaaa cccgcaccga tgacggatac cttgttgttc atgatttact
     1501 ccagcttccc cgtttttttg agtcgtaaag gtgaggcctt ataccataag aacagacgag
     1561 aaaaaaggag cttggggaca tttcagcttc ccttccccac cggcagccca tacttgtacg
     1621 ggtgtttttg gatgtccagg tatttggaac gggcgaagcc ggcggggaat ttctccttcg
     1681 agatgtaagg tagggcgtcg cgcttgaatt cctccatgaa ggtccggaag acgtgtccca
     1741 cctcccgatt ggggacgaag gtcctttctt tcttcagctc gtaataaacg tcgggcccga
     1801 atacctttgt cgcgagcctc agttgctctt cattcgagaa atactcctcc ttgaagatta
     1861 ctttaacacg aacgaggagg gcgaccttcg tcgtatcggc ggcgatggcc cgggagcggt
     1921 cccagacctc cgctacgagg ccgttgggca gctgtatctc ttcaaccatc ttcatgcttc
     1981 ttcaccgggg gctcaacttt ttcagccata cgagcgctct cacttttacc aagattattt
     2041 tttgctgccc gcgaacttgc catttctctt cactttctgt tgacctccgc ctccgttgcc
     2101 cctatactta gcacttaatt aacacaactg ccgaacgagg tgtcagatag attgagcgag
     2161 gccaacgcca tcctgcttct ttcctgtccc gaccagcgcg ggatcgtcgc atcggtctcc
     2221 aatttcgttt tccagcacaa cggcaacatc gtccacgcgg cccagcacac gacccagaag
     2281 tcaaagatat ttttcatgcg gatagagtgg gagctggaag ggttcgacat ccgccgggag
     2341 gatatcggga aggccttttc gcccatagcc gaccgcttcg gcatgacctg gtggctccat
     2401 ttcaccgatt atgtcccgag agtggcgatc ttcgtctccc gccacgtcta ctgcttccat
     2461 gacctcattc tgcgtcagaa catgggggaa atcaagtgtg agatacccat cgtcatcagc
     2521 aatcacatgg acctaaagcc cttatccgag catttcgggc tgaactaccg ctattacccc
     2581 gtcacgccgg aaaacaagga ggcgcaggaa gagcgggaaa tagaggagct ccgacagcac
     2641 cgggtggacc tgatagtcct tgcccgttac atgcagatac tcacgggtag gttcgtcgac
     2701 ctataccgcg accgcatcat caatatccac cattccttcc ttcccgcctt tgctggcggc
     2761 aagccctatg agcaggccta tgaccggggc gtcaagatca tcggtgccac gagtcattac
     2821 gtgacggaga gtctcgacga cggaccgatc atcgttcaag acgtgatcag gataagtcac
     2881 cgggattccg tggaagacat gaagatgaag gggaaggacc tggaaaggac cgtcctgtca
     2941 cgggcggtta aactccacct ggaacatcgg gtcatcgttc acgggtctaa gaccatcgtt
     3001 ttcgagtgat ccgccgagag cagactcaga aggtgaacat ggcgtcgcgc tccgcagcct
     3061 tgtacttcaa cctctcgtcc agatttttct tggcctccct cacagcgggg tggttcgggc
     3121 tgtactggag cgccttctcg taatcctcca tggcgctctt caattcgccc cgctcttgct
     3181 tcttgttgcc tctctccaag tagatgccgg cgaccccgtc ggccgcttcc ggaaaccccg
     3241 gtacgagttc gagggccttc aggtaatcgg caagggcacc ccggaaatcg cccaaccgtt
     3301 ctttctgatt ggccagcttg tagtatcccc tacccagggc ttctctgaat ttcgtcgagt
     3361 ccggtttcgc ctggagggcg aggtcgtagt ctgagacagc tccccggaaa tcattcaggg
     3421 tctccttcgc ccgtccccgt ctgaaaaaag cctcgcctct taaatcaccg tgacaggcgc
     3481 cgtctttttc caggaaacgg tcgtagaggt acacggcatc ttcgcatcgc ccgctgagac
     3541 aacggcggtc cgcctcccag aggtcctcct cacaggagct gccgggtttt actttctcgg
     3601 tcgccttccc ggcttcctgg gcagggcaga ctgccggact taggagaaga agcgaggaag
     3661 acaagaatat tgcaaagata acgcgaaaat tcctcctttt catggtatcc tacctacgga
     3721 agttcttttt tgtataccaa aaacagttca aaaaaggaag ccgccaagct gaaggcgtgg
     3781 aggggatcca tgacacttag gttgctggac ccggtcaggg agatactgaa agtcgttgaa
     3841 gagatgaccg acaggaaggt cgatttcgtt gaaaagaccg acctacccgt ttccgccgag
     3901 ttgaaggtgg ccgagaaggc tgagcaggcc catcgtctct tctaccggcc gacacatacc
     3961 gacgagatca actaccttgt agcccaccag tgcgggcatg ttctcaggct cttcggggcg
     4021 gagaagggaa aacgattcat ccctgtcgcc aatcgcaaga cgatgatgag ctatcttctg
     4081 gaaatggacg atgacctgaa acgtctttca atggtattcg gtcccgagaa ggtgaagaag
     4141 ctcgtttttc tctggtacga gggagtcgtc ttccaggtga ccaggatgcc ccccgacatc
     4201 atgatcgaca aatggctcta cgacgaatat cctgagttgc gggcgataca gctcgcctcg
     4261 cttgagaggc aaagaagggc ggcgatttta gggctttccg aggacatgag gaagataacg
     4321 cc
//
LOCUS       DAOTMX010000139         4310 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_16603_length_4310_cov_3.282726, whole genome shotgun sequence.
ACCESSION   DAOTMX010000139 DAOTMX010000000
VERSION     DAOTMX010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16603_length_4310_cov_3.282726"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="P5573_05045"
     CDS             complement(<1..300)
                     /locus_tag="P5573_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HRT27458.1"
                     /translation="MSNVGKPWGGRFNEPTNKLVEAFTASLPYDRRLYVYDIEGSMAH
                     CRMLSRVGIISKQEEKKILDGLKEVRKDFEEGRLDFSVDLEDIHMNIEAALIERAG"
     gene            complement(337..1563)
                     /locus_tag="P5573_05050"
     CDS             complement(337..1563)
                     /locus_tag="P5573_05050"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HRT27459.1"
                     /translation="MSDIKKVVVAYSGGLDTSVILTWLKETYGCEVIAFAADVGQKEE
                     LTGLEKKALDTGASKVYIDDLREEFVRDFVFPMLRADAVYEGTYLMGTSIARPLIAKR
                     QIEIAEAEKADAVCHGATGKGNDQVRFELTYMALNPSIKIIAAWRDPKWPFDSRQSMF
                     DYAEKHGIPLPLTKEKPYSSDRNLLHISHEGGILEDPWNEPGEEMFVLTVSPESAPDK
                     PTYVEIDFEQGNPVAVDGVKMSPAVLLDHLNDLAGRNGIGRVDMVENRFVGMKSRGVY
                     ETPGGTVLWAAHRAVESITMDREVMFLRDSLIPKYAQLVYYGFWFSPERLALQKMVDE
                     GQQAVTGTARLKLYKGNCIVVGRKSPLSLYSEDLASFEKSGLYDQKDATGFIRLNALR
                     LKMEALLRRKHGRQGT"
     gene            complement(1564..2493)
                     /gene="argF"
                     /locus_tag="P5573_05055"
     CDS             complement(1564..2493)
                     /gene="argF"
                     /locus_tag="P5573_05055"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HRT27460.1"
                     /translation="MMRKDLISFAGLDRRDFDRIFERAFAIKRMQKEGVVYTPLKGKT
                     LGMTFFKSSTRTRLSFEVGMYQLGGIAIFLSARDIQVGRGESIEDTARIMSRYLDGVV
                     VRTFSQESVEEFARAASIPVVNGLTDLLHPCQILSDLFTISEKKGTYEGVKVAYVGDG
                     NNIANSLIEAATRLPFELALACPEEYEPNPEILRAGVQSSGGRVRLFRQPSEAVEGAD
                     VVYTDVWASMGQEGEAEKRKQALKDYQINAALMKRAKRDAVVMHCLPAHRGEEITADV
                     IDGPQSVVFDQAENRLHVQKAILEILMGGGKET"
     gene            complement(2490..3689)
                     /locus_tag="P5573_05060"
     CDS             complement(2490..3689)
                     /locus_tag="P5573_05060"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HRT27461.1"
                     /translation="MSTEELIAQSKRYIMNTYRRFPIVLVRGEGIRVWDSDGKEYLDF
                     VAGIAVCSLGHAHPKVVAAIKRQAEILCHVSNLYHIEPQVRMAKLLVECSFADKVFFC
                     NSGAEANEGAIKLARKYGREKLGKDRYELITMKGSFHGRTLATLTATGQERFHAGFEP
                     LPAGFRYVPFDDLRSLEEAVTEKTVGVLVEPIQGEGGVRVPTEDYLRGVRKICDEKKI
                     LLILDEVQVGMGRTGPLFAHEDYGIKPDIMTLAKAMGNGFPVGALLATEEVADAFVPG
                     THASTFGGNPLAMAAAVATLQTILHDGVLENCRAVGTYFFQELNSMKERFPFIEEVRG
                     KGLILGVELNIDGQEIVDRCLENGLLINCTAGNILRFVPPLTITKAEVDKGLGIIEGV
                     LHEVSGR"
     gene            complement(3710..>4310)
                     /gene="argB"
                     /locus_tag="P5573_05065"
     CDS             complement(3710..>4310)
                     /gene="argB"
                     /locus_tag="P5573_05065"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HRT27462.1"
                     /translation="IVEMVLVGKVNKEIVGLINHHGGKAVGLSGKDGSLIQAEKYYLN
                     EEKAKDTPPEIIDIGLVGKVAAIDANLIVSLAKDGFIPVIAPTGVGAKGETYNINADI
                     VAGEVAAALKAEKLVLLTDVEGVMDEKKDLINTMDGGRASNMIENGTIAGGMYPKVKC
                     CLKALRQGVRKTHIIDGRLKHAVLLEIFTDRGIGTEIVL"
BASE COUNT          841 a         1351 c         1047 g         1071 t
ORIGIN      
        1 gccggcccgc tcgatgaggg cggcctcgat attcatgtga atgtcttcga ggtcaacaga
       61 aaagtccagt ctaccttctt caaagtcttt ccggacctcc ttaagcccgt cgaggatttt
      121 cttttcttcc tgtttcgaga ttatacccac cctggagagc attctgcagt gggccatgct
      181 cccttctatg tcgtaaacgt aaaggcgccg gtcatagggt agagacgccg tgaaagcctc
      241 cacaagtttg ttcgtcggtt cgttgaaccg cccgccccat ggttttccga cgttgctcat
      301 ctcttttctc cggtcgaatc catctgcgga cggaccttat gttccctgcc gaccatgttt
      361 ccttcgcaaa agtgcctcca tcttcagcct cagggcatta agacggatga acccggtggc
      421 gtctttctgg tcgtaaaggc cgctcttctc gaagctcgcc aagtcttcgc tgtaaaggga
      481 taacggcgac ttccgaccga cgactatgca gttgccctta tacaacttga ggcgagcggt
      541 tcccgtcaca gcctgttgcc cttcgtcaac catcttctgt agcgccaacc tttccggtga
      601 gaaccagaac ccgtagtaga caagctgcgc atatttcggg atgagggaat cccgcaaaaa
      661 catcacttcc cggtccatag ttatggactc gaccgcccgg tgggccgccc acagcacggt
      721 gcctcccggc gtctcgtaaa caccgcggga cttcatgccc acaaacctgt tttccaccat
      781 gtcgacccgg ccgatgccgt tcctgcccgc aagatcgttc agatggtcca ggaggacggc
      841 ggggctcatc tttaccccgt ctacggctac cggatttccc tgctcaaaat cgatctccac
      901 gtaggtcggc ttgtccggtg ccgactcggg cgacaccgtc aggacgaaca tttcctctcc
      961 cggctcattc caaggatcct cgaggatgcc gccctcatga gagatgtgga gtaggttcct
     1021 gtcgctgctg tatggcttct ctttcgtgag cggaaggggt atcccgtgtt tttctgcata
     1081 atcgaacatg gactgccggg aatcgaaggg ccatttgggg tcccgccacg ccgcgatgat
     1141 tttgatggac ggattaaggg ccatgtacgt cagctcaaac ctcacctgat cgttaccctt
     1201 gccggttgcc ccgtggcaga cagcgtcagc tttctcggcc tcagcgatct ctatctgtct
     1261 cttggcgatg agaggccgcg cgatcgacgt tcccataagg taggtgcctt cgtagacggc
     1321 atcggccctc agcatgggga acacgaagtc gcggacaaac tcctctctaa ggtcatcgat
     1381 gtacacttta gacgcgccgg tgtcgagggc cttcttctcg aggcccgtca gctcttcttt
     1441 ctgtcccaca tccgccgcaa aggcaatcac ctcacatcca taggtctcct taagccacgt
     1501 caggatgacc gacgtatcca gtccccccga ataggccaca actacctttt taatgtcact
     1561 catctatgtt tcctttcctc ctcccatgag aatctccagg atcgccttct gaacatggag
     1621 gcgattttca gcttgatcga agacgaccga ttgcggaccg tctatcacgt cggccgttat
     1681 ctcttcaccc ctgtgggccg gcagacaatg catgacgacg gcatccctct ttgccctttt
     1741 catcaatgcc gcatttatct gatagtcttt gagggcctgc ttgcgctttt ctgcttcacc
     1801 ttcttgcccc atgctcgccc agacgtctgt gtagaccaca tccgcccctt ccacggcctc
     1861 ggacggctgg cggaataatc taacccggcc gcccgacgat tgtacgcccg ccctcaggat
     1921 ctccggattc ggttcatact cttccgggca agcaagcgcc agctcgaagg gaagtctcgt
     1981 cgccgcttct atcagagaat tggcgatatt gttcccgtct cccacgtatg ccactttaac
     2041 gccttcgtag gtgccctttt tttcgctgat tgtaaacaga tcgctcagta tctggcatgg
     2101 atgcaggagg tccgtcagtc cgtttacgac cgggatcgac gcagcccttg cgaactcctc
     2161 taccgattcc tgtgaaaagg tcctgaccac caccccgtcc agataccggg acatgatccg
     2221 tgccgtgtcc tcgatcgatt cccccctgcc gacctggatg tcacgggcgc ttaaaaagat
     2281 agcgataccg ccgagctgat acatcccaac ttcgaaagaa agcctcgttc tcgtggatga
     2341 cttgaaaaag gtcatcccga gtgtctttcc cttcagagga gtgtaaacga ctccctcctt
     2401 ctgcatcctt ttgatcgcga aggcccgctc aaagatgcgg tcgaaatcac gtctgtctaa
     2461 gcctgcaaaa ctaatcaggt ctttcctcat catcgccctg acacctcgtg caaaacgccc
     2521 tcgattatcc ccaacccctt gtcgacttcg gccttagtga tggtcagggg cgggacgaac
     2581 ctcaggatgt tgcccgcagt acagttgatg agcaaaccat tttccaggca ccgatcgacg
     2641 atttcctggc cgtcgatgtt cagctccacg cccaggatca accctttacc cctcacttct
     2701 tcgatgaagg gaaaacgttc tttcatagag ttgagctctt gaaaaaaata ggtcccgacg
     2761 gccctgcagt tttccagtac gccgtcatga aggatggtct gcagtgtggc aacggcggcg
     2821 gccatcgcca gcgggttccc gccgaaggtc gaggcatggg tccccggcac aaaagcatca
     2881 gccacctctt ctgtggcgag aagcgccccc acaggaaaac cgtttcccat tgcctttgcc
     2941 agcgtcatta tgtccggttt tatcccgtaa tcttcatggg cgaaaagggg tccggtcctg
     3001 cccatcccga cctggacctc gtcgaggatg aggaggattt tcttctcgtc acagattttg
     3061 cgcacccccc gcagataatc ttcggtcggt accctgaccc ccccctctcc ctggatgggc
     3121 tccaccaaaa ctccgaccgt cttctcagtc accgcttctt caagggatcg aaggtcgtcg
     3181 aaaggcacgt acctgaatcc cgcggggagc ggctcgaagc ctgcgtgaaa tctttcctga
     3241 ccggtcgcgg taagcgtcgc cagggtcctt ccgtgaaagg accctttcat ggtgatcagc
     3301 tcgtatctgt ctttccccaa tttttcacgg ccgtactttc gcgctagttt gatcgcaccc
     3361 tcgttggctt ctgcaccgct gttgcagaaa aacaccttgt ctgcgaaaga gcactcaacc
     3421 agcagctttg ccatgcggac ctgcggctcg atgtgataga ggttggagac atgacacaga
     3481 atctccgcct gccttttgat ggccgccacg accttgggat gagcatgtcc gaggctgcac
     3541 acggcgatcc cggcgacgaa gtcgaggtat tccttaccgt cactgtccca tacccggatg
     3601 ccctcacctc tcacgaggac gatcgggaat cgtctgtagg tgttcattat atatcttttg
     3661 gactgggcta tcagttcttc cgtgctcatc ctcagaaacc ttccttttcc tacagaacta
     3721 tctcggtccc gatgcccctg tcggtgaaaa tttcgagcaa tacggcatgc ttcaatcgcc
     3781 cgtcgatgat gtgcgttttc ctaacaccct gtctcaaggc cttgagacag catttcacct
     3841 tgggatacat gccgccggcg atcgtcccgt tctctatcat gtttgaagcc cgcccgccgt
     3901 ccatggtgtt gatgagatct ttcttctcat ccatcacccc ctccacatcc gtcaggagaa
     3961 cgagcttttc cgccttcagt gccgcggcca cttcacccgc cactatgtcg gcgttgatgt
     4021 tgtatgtttc gcccttcgcg ccgacgccgg tcggggcgat gaccgggatg aaaccgtctt
     4081 tcgcgagaga gacgatgagg ttcgcgtcga tggccgccac cttacccaca aggccgatgt
     4141 cgatgatctc aggcggtgtg tcttttgctt tttcctcatt caggtaatac ttttcggcct
     4201 ggatcagact tccgtcctta ccgctcaacc cgaccgcttt cccgccgtgg tgattgatca
     4261 agcccacgat ctctttgttg accttcccca ccaataccat ttctacgatg
//
LOCUS       DAOTMX010000140         4285 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_16719_length_4285_cov_4.260284, whole genome shotgun sequence.
ACCESSION   DAOTMX010000140 DAOTMX010000000
VERSION     DAOTMX010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4285
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16719_length_4285_cov_4.260284"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(245..1081)
                     /locus_tag="P5573_05070"
     CDS             complement(245..1081)
                     /locus_tag="P5573_05070"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HRT27463.1"
                     /translation="MGEENEKRSADEKGEADVAGKIWTEGHKVRSYEADPHGNITVLS
                     LYNYLQEAAANHARALGVSIHEIISDNITWVLSRLALRVDAYLSWGDRINVRTWPSGV
                     RGPFALRDFLVTGEDGSSVAAAVSAWLLIDAKTRMPLRRVRSVLERLHPPAVNPPLDN
                     PLGKLERIDGCDRQEIFTVQYDDLDINRHANSACYIRWIMGSLPPEIPEGAVLADLEV
                     NYLTEAFLGEEIVARSGKAAGDPLSFFHLLLRKRDGQELLRARTSWKPRKERTARSSG
                     MS"
     gene            1275..2453
                     /locus_tag="P5573_05075"
     CDS             1275..2453
                     /locus_tag="P5573_05075"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HRT27464.1"
                     /translation="MKRRGRIKVLQGKMREAGLYGVLLFYSRDIFYYTGTAQPSYLAV
                     STVDYRLFVRSGFDFALNDVFIDPEKMKEERRLENIRREFFPDIREGTKVATETDVLT
                     VEQWHQFRKAFPGCEFVNVSPLVLGQRMAKEPGEVECIRKACDAVHRGHEAVLANLRE
                     GMTELELSAIVENAHRLAGHEGIFFMRQPDFFMSRGPISSGSNLFKVSGVVYTITGVG
                     LSASVPAGPSRRKIKRRDLIMVDIPTHVDGYHADQTRTYCLGKAPEAVHAMYDDLRAI
                     ADFLIQEMRAGMRCDEIYRLALAKAEELGRSGQFQGFGSGKRGRLIGHGIGLELNEPP
                     IPSEYDRSEIAENYVIALDIHMMDEALGVVKLEDMILIKKEGNEILTKSPRELFEVGY
                     "
     gene            2557..3459
                     /locus_tag="P5573_05080"
     CDS             2557..3459
                     /locus_tag="P5573_05080"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRT27465.1"
                     /translation="MVNNTVKMMVAALLVLFVSAGAASAADEVLARIGNRVVTQKDIE
                     YLIALYPENEQALLRGSVEYREVLLKNMVTVIVVSDIAKKKNFDKKGDIGKQVELIMN
                     EYLARAYVEQEILGGVTVSDEEIEKYYRVNPSAFESPERLRVRHILITVKPGASEGEK
                     NAAKKKTTDILERAKRGADFAKLAEEYSEDPGSRQKGGDLGYITRKTTMPELEDAAFA
                     LEVGEVGGPIETAFGFHLIKVEEKKKASVAPFDQVKEEARAKTLQEAKQKKVNSFIDQ
                     AFKDAGVKFVAPVGEKKNSLSLTP"
     gene            complement(3473..3838)
                     /locus_tag="P5573_05085"
     CDS             complement(3473..3838)
                     /locus_tag="P5573_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27466.1"
                     /translation="MAEEKSTKTKGAFIGRSPEAAGEQAARRWREDLTRFFDENDVGL
                     DALWLGKDLDDFDYCYLMSGYEVYSLFPGLPEAGVSVMMDASLTRFLRSEEGLVSFAH
                     LYGGSGSFRYALPHESDLA"
     gene            complement(4098..>4285)
                     /locus_tag="P5573_05090"
     CDS             complement(4098..>4285)
                     /locus_tag="P5573_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27467.1"
                     /translation="LSHHDLIDKLKDIVVTATQEYKTSAVDYNIVFTLEFLFEDGVDP
                     DMKVSFIRELQEFFGKF"
BASE COUNT         1063 a         1132 c         1245 g          845 t
ORIGIN      
        1 ggcgagcttc ccttacgcca ccaagacctc catgcagctt gactacgaaa aggggcggcc
       61 gacggaaatc gatatcttca tggaatacat catgaatgcc ggaaaggaat tggggatcgc
      121 cactcccatc cacgaggaag tctacagggg gttgatggaa agaaggtgag acagccccgg
      181 ccccaccgtg ctgaataaaa aaaccggcgt attcggtacg gtacggtggg aaaagatagg
      241 gtcgtcaaga cattccagag gaccgggccg tcctttcttt tctcggcttc caggatgtcc
      301 tcgcccgcag aagctcctga ccgtcccgct tccgcaagag gagatgaaag aaggaaaggg
      361 gatctcccgc cgccttcccg gaccttgcta cgatttcctc gcccaaaaag gcctcagtga
      421 ggtagttgac ctccaggtct gccaacaccg ccccttccgg tatctccggc ggcaggctcc
      481 ccataatcca gcggatgtag caggcgctgt tggcgtgacg gttgatgtcg aggtcgtcgt
      541 actgtacggt gaaaatctct tgccggtcac agccgtcgat tctttcgagt ttgcccaggg
      601 gattgtcgag gggagggttg acggcagggg gatgaagccg ctcgaggacc gaacggacgc
      661 gccgcaaggg catccttgtc tttgcatcga tcagaagcca ggcgcttacc gcggcggcga
      721 cggaggagcc gtcctcgcct gtcacgagga agtccctgag agcgaagggc ccgcggacgc
      781 cggaaggcca ggttctgacg ttgatacggt ctccccaact cagataggca tctaccctca
      841 acgcgaggcg ggaaaggacc caggtgatgt tatcagagat aatctcatgt atcgaaacgc
      901 caagggcacg ggcgtgattc gccgccgcct cctgaagata attgtagagg gaaagaacgg
      961 tgatattccc gtgagggtcg gcctcgtaag agcggacctt gtgaccttca gtccagattt
     1021 tcccggcgac gtctgcctct cctttttcgt ccgccgatcg tttttcgttt tcttctccca
     1081 tgattgaatc cagagggaat agctcagact gttgcagcgg gattatattc aaagcccgcg
     1141 ggcgagtcaa tgcccaagga gtcgtggaaa aggttgcacg agaaggcgat ccatggttaa
     1201 ctctgtggag atgctcaaac tacacagtga tagatgatga gtgataaccg gttgtttagg
     1261 gatgggaaca ggcgatgaaa cgcaggggac ggataaaggt cttacagggc aagatgcgag
     1321 aagctggcct ctacggcgtc cttctcttct attcccggga catcttctat tacacgggga
     1381 cggcgcagcc ttcctacctc gcggtctcga cggtggacta ccgcctgttc gtgaggagcg
     1441 gctttgactt tgctttgaac gatgttttca tcgacccgga gaaaatgaaa gaggagcggc
     1501 ggctcgaaaa catccgccgg gagttcttcc ccgatatccg ggaaggaacg aaggtcgcga
     1561 cggaaacgga cgtcttgacc gtagaacaat ggcaccagtt caggaaggcc tttcccggat
     1621 gcgagtttgt caacgtctcg ccgctggtcc tcgggcagag aatggcaaaa gaaccggggg
     1681 aggtcgaatg catacggaag gcatgcgatg cggtccatag aggccacgag gccgtcctcg
     1741 cgaacctgcg cgagggcatg acggagttgg agctttcggc aatcgtcgag aacgcacacc
     1801 ggttggccgg tcacgaaggg atctttttca tgagacaacc ggatttcttc atgagccgtg
     1861 ggcctatctc ttcggggtcc aatcttttca aggtgagcgg cgtcgtgtac accataaccg
     1921 gggtaggatt gagcgcctcc gttccggccg gaccttctcg aagaaagatc aaacgcaggg
     1981 acctgataat ggtcgacatc cccacccacg ttgatggata ccacgcggac cagacgagga
     2041 cgtactgtct cgggaaggcg cccgaggccg tccatgccat gtacgacgac ctgagggcga
     2101 tcgccgattt cctgatccag gagatgaggg cgggtatgag atgcgacgag atataccggc
     2161 ttgccctcgc aaaagcagaa gaactcggac gctccggtca attccagggc ttcggctccg
     2221 ggaaaagagg gcgtcttatc ggccacggca taggcctgga actgaatgaa ccaccgatcc
     2281 catccgaata cgaccgatcc gagatcgcgg aaaactacgt catcgccctc gacatccaca
     2341 tgatggacga agccctcggt gtcgtcaaac tcgaagacat gatcctgatc aaaaaagaag
     2401 gaaacgaaat cctgacgaaa agcccccggg agcttttcga ggtcggatat taacgacggc
     2461 gggagagcga tcgtggggaa gatgcctttg gagaatcggg acatccctct cacggttctg
     2521 acggcttttg ttcgtagtca ataaaggaga ggtgcgatgg taaacaacac cgtaaagatg
     2581 atggttgcag cgctgcttgt cctcttcgtc tcggccggcg ccgcttccgc ggcggacgaa
     2641 gtcctcgcgc gcatcggaaa cagagtggtc acccagaaag acatcgaata tctcatagcg
     2701 ctgtaccctg aaaacgagca ggccctactc aggggaagcg tggagtaccg tgaggtgctc
     2761 ttgaagaaca tggtcaccgt catcgtcgtc tccgacatag ccaagaagaa gaacttcgat
     2821 aaaaagggag acataggaaa gcaggtcgag ctcatcatga acgaatacct cgcccgggct
     2881 tacgtcgaac aagaaatcct gggcggagtg acggtgagcg atgaggaaat cgaaaaatac
     2941 tacagagtca acccgtcggc cttcgaaagt ccggaacgac ttcgcgtcag gcacatactg
     3001 atcaccgtca agccgggggc gtccgaaggg gagaagaacg cagccaagaa aaagacaacg
     3061 gacatcctgg agcgggccaa aaggggagcg gattttgcca aactggccga agagtattcc
     3121 gaagatcccg gttcaaggca gaagggcggc gacctgggtt acatcacaag gaagactacg
     3181 atgccggaac tcgaagatgc cgctttcgcg ctggaagtcg gggaagtcgg cggtcctatc
     3241 gagactgcct tcggctttca tctcatcaag gtggaagaga aaaagaaggc ctctgttgcg
     3301 ccctttgacc aggtaaaaga agaagcccgg gccaaaacgc tccaggaggc aaaacaaaag
     3361 aaggtaaaca gcttcatcga ccaggccttc aaggacgccg gcgtgaaatt cgtcgcacca
     3421 gtaggggaaa agaaaaactc cctgtctttg accccttagc ccgtgccttc tcctatgcca
     3481 ggtcgctttc gtgcggaagg gcataccgaa aacttccact ccctccatag agatgggcaa
     3541 agctgaccag accctcttcc gaacggagaa aacgggtaag acttgcgtcc atcataaccg
     3601 atacgcccgc ttccggaagg ccgggaaaca gactgtagac ctcataaccg ctcatgaggt
     3661 aacaatagtc aaagtcatcg agatcttttc caagccagag ggcatccagt cccacgtcgt
     3721 tttcgtcgaa aaacctggta aggtcctccc gccaccggcg agccgcttgc tccccggctg
     3781 cttcgggact ccgcccaatg aaagctcctt tggttttcgt tgacttttcc tctgccatga
     3841 catcctccag tttattttag gataacggcc tgagactgag gtaatcataa tccgtgccag
     3901 aggaagcaca cgggttaatc tattccaagt actgaacttt actgataatg agagatcgga
     3961 cagacgtcaa gggtccccga ggccggcttg tgaccgcgta acggtcttcg cagtgggaag
     4021 atttttgcgc atacccaaca tgtgaggtgg tggataccgt gccgaaggat caagtcagga
     4081 aaacaactgg tggggggtca gaacttaccg aaaaattcct ggagctcccg gatgaacgag
     4141 actttcatgt ccgggtcaac accatcctcg aacaggaatt ccagggtaaa gacgatgttg
     4201 tagtccacgg cggatgtctt atactcttga gtcgccgtga cgacgatgtc cttcagctta
     4261 tcgatgaggt cgtgatgaga caggt
//
LOCUS       DAOTMX010000141         4253 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_16868_length_4253_cov_3.229157, whole genome shotgun sequence.
ACCESSION   DAOTMX010000141 DAOTMX010000000
VERSION     DAOTMX010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4253
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16868_length_4253_cov_3.229157"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /locus_tag="P5573_05095"
     CDS             <1..966
                     /locus_tag="P5573_05095"
                     /inference="COORDINATES: protein motif:HMM:NF025099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA 5TM domain-containing
                     protein"
                     /protein_id="HRT27468.1"
                     /translation="FTFRGVEIPMYSEFSPILIGGKPTPFWPAVPLIIACGSLSGFHS
                     LVASGTSSKQIAKESDGLFIGYGAMFTEGFLSTIIICAIAGYGFTAMKNAAAAANVPL
                     ALNLDNWGALFTKVSEQFKLSQANLVVQTYADMVAASFLSFIPTKLVKVICGLWISAF
                     ALTTLDTTNRIARYCLVEMMAPVKDAMTGVYNVLTNRWIASIIPAAIGIYLAWSKQFT
                     IVWPSFGAANQLIASIALMTGAAFVAKKLKSKFAIVAVVPAWFLWVTVTCAIVWFMIV
                     VMPANIAKAPGQGWTVQAIMAIMLILNFIFIWDFIKSRKKTEEGT"
     gene            1047..1328
                     /locus_tag="P5573_05100"
     CDS             1047..1328
                     /locus_tag="P5573_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27469.1"
                     /translation="MAASIIDRIGRFLSVFRAAHREKVIALTCVELNELENIFALLLL
                     GSFVGFPAPPTFLSVELLPFMEREMSVIHQRAEDACDMLAQMVGTLGIE"
     gene            1328..2293
                     /locus_tag="P5573_05105"
     CDS             1328..2293
                     /locus_tag="P5573_05105"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HRT27470.1"
                     /translation="MKILFYTGKGGVGKSTMAAATAWQLSRHHRVLAVSLDPAHNLGD
                     IFRVSLKDGEKRLTENLHLREVDLKKLTRDYLEREVTVLTDTYKYLQVLNLDRYFSIL
                     KYSPGIEEYALLTSIEETVREEKGFDYVIFDTPPTGLTLRFLALPQVTIAWIDRLTHI
                     RRQILEKRYTIQKVRGGLPEGGKNPEIRLRFDEDEDGVIRRLKALKQNYGALTGILQG
                     GDCGVILVFNPDLLSLRESVRLIEGLKELKLPLRLLIDNKVTEENREMASQVEQEFKK
                     IAGGIPMQRIALSEALYRGAEKNLFDIREDLLAHLQWGVRKDVRA"
     gene            2280..2951
                     /locus_tag="P5573_05110"
     CDS             2280..2951
                     /locus_tag="P5573_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27471.1"
                     /translation="MFEHEYIATQPLFYLFIAFSVLLAYGYFWGKRMNHRLFLSAFND
                     LVDVIQPADQTFTNIGGLIGYHANFLTKKSSIAKVDATITFLPRQSWLYFPVSKLIRR
                     WDRLFITLYLRQAPSEEGHLIEAGYAAFRGPKITNADRLNREKIKWGEQDFYLYYQGM
                     KMRDALLGFMEKNGDPGIIRHIAIVPEQQRCFVFMIPRKGEVGRYFGPIYRWVPSLVR
                     ETGCS"
     gene            3135..3656
                     /locus_tag="P5573_05115"
     CDS             3135..3656
                     /locus_tag="P5573_05115"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HRT27472.1"
                     /translation="MGTSQQMDVFGYALNAERDGMNFYLKAARKFKGEEDLKDVFSRL
                     AKEEAKHMETFLGLRAKMEGKDIGECFRIPEVGGYLESVIQEGLFPKGENATKRLEKI
                     KSVGEACAVAMQAEKNSILLYSELSKLTKDKEQKKILDKIAKEEKAHLVMVRGLRADF
                     DPMYAALKFGRFF"
     gene            complement(3675..3947)
                     /locus_tag="P5573_05120"
     CDS             complement(3675..3947)
                     /locus_tag="P5573_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27473.1"
                     /translation="MKPEMIQKIDAVLDLVKDPESNLPVAQLGVVKRVRYNEELKTLY
                     VFTEFYSHLPKCVTCAAIASAIASTILQDLREEFQAEFPDLSIEFV"
     gene            4033..>4253
                     /locus_tag="P5573_05125"
     CDS             4033..>4253
                     /locus_tag="P5573_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HRT27474.1"
                     /translation="MRETRVSRIIKSEDLNHHGTLYAGRMAEWLVEACLITTINLTRK
                     PEDVVCVRIHGFDFKKPAMSGDVIDIRARV"
BASE COUNT          937 a         1162 c         1215 g          939 t
ORIGIN      
        1 ttcaccttcc ggggggtcga gattcccatg tattcggagt tctcgccaat cctgataggc
       61 gggaagccca cacccttctg gcccgctgtg cccctgatca tcgcctgcgg ctcgcttagc
      121 ggttttcatt ctctcgtggc ctcggggacg tcgtcgaagc agatcgccaa ggagtcggac
      181 ggtctcttta tcggttacgg cgccatgttc accgaggggt ttctctccac gatcatcatc
      241 tgcgctattg ccgggtacgg cttcacggcc atgaagaacg cagcggcggc ggccaacgtc
      301 cccctggccc tgaacctcga caactggggg gccctcttca ccaaggtgtc agagcagttc
      361 aagctctccc aggccaacct ggtcgtccag acctacgccg acatggtcgc ggcgagcttc
      421 ctctctttca ttccgacgaa gctcgtcaag gtcatctgcg gtctctggat ttcggccttc
      481 gcgctcacga cgctcgacac gacgaaccgg atcgcccggt actgcctcgt ggagatgatg
      541 gccccggtga aggatgcgat gacgggggtt tacaatgttc tgaccaaccg ctggatagcc
      601 tccatcattc cggctgccat aggcatctac cttgcctgga gtaagcagtt cacgatcgtc
      661 tggccctcct tcggcgcggc caaccagctg atcgcctcca tcgccctcat gacaggcgcg
      721 gccttcgtag cgaagaagct caagtcaaag ttcgccatag tggcggttgt tccggcttgg
      781 ttcctgtggg tcaccgtcac ctgcgcgatc gtctggttca tgatcgtcgt catgccggcc
      841 aatattgcca aagcccccgg ccagggctgg acggttcagg cgatcatggc gatcatgctg
      901 atcttgaatt tcatctttat ctgggacttc atcaagtcga gaaagaagac cgaggaaggg
      961 acataagtta gtacttgacc atgcgaaccg gtgtgtgctg aaattcgggt gcacaccggt
     1021 ttttttgatc aaaggggacc tgttcaatgg cggcatctat catcgaccga atcggccggt
     1081 tcctttctgt tttccgcgcg gctcaccgcg agaaggtcat cgcccttacc tgcgtggaat
     1141 tgaatgaact ggagaatata ttcgccctcc ttctcctggg ctccttcgtc gggttcccgg
     1201 cgcctccaac cttcctttcc gtggaacttc tccccttcat ggaacgggag atgtcagtca
     1261 ttcatcagcg ggccgaggat gcctgcgaca tgctggcgca gatggtgggg accctgggaa
     1321 tcgagtgatg aagattctct tttacacggg aaaggggggg gtgggcaagt ccaccatggc
     1381 ggcagccacg gcctggcagc tttcacggca ccaccgtgtc cttgccgttt ccctggaccc
     1441 ggctcacaac ctcggggaca tcttccgcgt ttcgttgaaa gacggcgaga agcgactgac
     1501 ggagaacctt cacctcaggg aggtggatct caagaaactc acccgcgact atctggaaag
     1561 ggaggtcacg gtcctaaccg acacgtacaa ataccttcag gtcctgaacc tcgaccggta
     1621 tttctccatc ctgaaatact cgccggggat cgaggagtat gccctcctca cgagcatcga
     1681 ggagacggtc cgggaagaga agggctttga ttacgttatc ttcgacacgc ccccgacagg
     1741 attgaccttg cggtttctcg ccctgcccca ggtgacgatc gcctggatcg accggctgac
     1801 gcatatccgg aggcagatcc tggagaagcg ctacacgatc cagaaggtcc ggggggggct
     1861 gccggagggt gggaaaaacc cggagatacg cctcagattc gacgaagatg aagatggggt
     1921 catcaggcgg ctcaaggcgc tgaagcaaaa ttatggagcg ctgaccggaa tccttcaggg
     1981 tggggactgc ggggtgatcc tcgtcttcaa ccccgacttg ctctcgctcc gggaatcggt
     2041 tcgcctgatc gaaggattga aggaattgaa gctacccttg cgccttctca tcgataacaa
     2101 agtgactgaa gagaaccggg agatggcctc gcaggtggag caggagttca agaaaatagc
     2161 gggaggaatt cctatgcaaa ggatcgcgct ttccgaggcc ctctaccgcg gcgcggagaa
     2221 aaatctcttc gatatccgtg aggaccttct tgcccatctg cagtggggag tgagaaaaga
     2281 tgttcgagca tgaatatata gcgacgcagc cccttttcta tctcttcatc gccttttccg
     2341 ttctgttggc ctacggctat ttttggggga agcgcatgaa tcaccggctc ttcctctccg
     2401 ccttcaacga tctcgtggac gtgatccagc ctgccgacca gacattcacc aatatcgggg
     2461 ggctcatcgg ctatcatgcc aacttcctca cgaagaagag ctccattgcc aaagtggacg
     2521 cgaccatcac gtttcttcca cggcaatcct ggctctattt tcctgtctcc aagttgattc
     2581 ggagatggga ccgtctcttc atcaccctct acctgcgcca ggccccgtcg gaagagggac
     2641 acctgataga ggccgggtac gccgccttcc gcggaccaaa aatcacgaat gccgaccgtc
     2701 tgaaccggga aaagataaag tggggcgaac aggacttcta tctctattac cagggcatga
     2761 agatgcggga tgccttgctc ggattcatgg agaagaacgg tgaccccggg atcatccgcc
     2821 acatcgctat cgtccccgag cagcagcgat gctttgtttt catgatcccc cggaagggcg
     2881 aggtgggtcg gtacttcggg ccgatctatc gatgggtgcc ttccctcgtc cgggaaaccg
     2941 gctgctcgta agggcgtttg aaccctcgaa ccggcgcatg cgtcgtccac cccggcggtc
     3001 ggctttatat aacgatcggt aagtgttttt tcttgacaga caggggtccc tataggacat
     3061 taattttcat ggcgacatga caggtgagtc tggaaagacc attaacccaa ccaacggaga
     3121 gaggaggtaa gaccatggga acgtcacagc aaatggatgt ctttggttat gccctcaacg
     3181 cggaacggga tggcatgaat ttctacctga aagcagccag gaagttcaaa ggggaagaag
     3241 atctgaagga tgtcttttcc cgattggcta aagaagaagc caagcatatg gagacgttcc
     3301 tcggtctaag ggcgaagatg gaaggaaagg acatcggcga atgtttccgc attccggagg
     3361 tcggcggtta cctggaatcg gtcatccagg agggcctttt ccccaagggt gagaacgcga
     3421 ccaagcggct cgaaaagatc aaatccgtcg gggaggcctg cgccgtggcc atgcaggcgg
     3481 aaaagaattc catcctcctc tattccgagc tgtcaaaact gaccaaggac aaggaacaga
     3541 aaaagatcct cgacaagatc gccaaagagg agaaggccca cctggtcatg gtcagaggtc
     3601 tgagggcgga ttttgatccc atgtatgcgg ccctcaagtt cggacggttt ttctaacggc
     3661 tttaccggag atcgtcaaac aaactcgatg gagagatcgg ggaactcggc ctggaattcc
     3721 tcccggagat cctgtaggat cgtggatgcg atggcggagg cgattgccgc gcatgtgacg
     3781 catttcggga ggtggctgta gaattccgtg aatacgtaaa gggtcttcag ttcttcgttg
     3841 taacgaaccc tcttgaccac gccgagctgt gccacgggaa ggttgctctc cgggtccttg
     3901 accaggtcga ggacggcgtc gatcttttga atcatctccg gtttcatgtg aataacgtac
     3961 agttaatatc ctacgttgtc aaaagattat cggcgacggt cctgttgacc ggacgggaga
     4021 aaggtttctg aaatgagaga aacacgagtt tcaagaatca tcaagtcaga agacctgaat
     4081 catcatggca cactttatgc cggccggatg gcggaatggc tcgttgaggc ctgccttatc
     4141 accacaatta acctgactcg gaagccggag gatgtcgtct gcgtccgcat ccatggtttc
     4201 gatttcaaaa aaccggcgat gagcggggat gtgattgata tccgggcaag ggt
//
LOCUS       DAOTMX010000142         4228 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_16992_length_4228_cov_3.586149, whole genome shotgun sequence.
ACCESSION   DAOTMX010000142 DAOTMX010000000
VERSION     DAOTMX010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4228
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16992_length_4228_cov_3.586149"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            210..623
                     /locus_tag="P5573_05130"
     CDS             210..623
                     /locus_tag="P5573_05130"
                     /inference="COORDINATES: protein motif:HMM:NF018391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HRT27475.1"
                     /translation="MIIYDLKCEKNHRFEGWFDDRAAFEAQKSGKFISCPVCGSTDIE
                     IIPSTVAVLGKDSRNDAGDRREVYPLKAAQLLQEYLNTNFDDVGDRFAEVALKIHKGE
                     EEKRNIKGTTTKEEEETLRETGVQFFKVTVPKLDS"
     gene            689..2998
                     /gene="cysS"
                     /locus_tag="P5573_05135"
     CDS             689..2998
                     /gene="cysS"
                     /locus_tag="P5573_05135"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HRT27476.1"
                     /translation="MEKRDSDILGMIGNTPLVELRKLNFNKRVKVLAKLESFNPGGSI
                     KDRPALFMIVKAEERGELTKGKTILEATSGNTGIGLAMIAAVKDYKLLLMMPESASEE
                     RKKILKALGAELRLTPAALGTDGAIEAAYNLAREQPDKYFLVDQFNNEDNIAAHYHGT
                     AEEIWRQTEGKVTLVVCALGTSGTAMGISRRLKEYNPDVKVVGVEPYLRHKIQGLKNM
                     KESYVPGIFDKNRLDEKVNILDEDAFTMARRLLREEGILAGMSSGAAAHVALEKAKEM
                     DGGVVVVIFPDGAERYLSTELFADREKTTLRIWNTPTRKKDFFVPVDPEEIRVYSCGP
                     TVDLVYHVGSYRRFVVSDMVVRYLESKGYRVKHVIDFIDLADRAIKGAEQAGRDLKDF
                     TEHCFQEFLKGTETLNIQKGALFPKASDNIDEMVDLVEKLTEKGYAYEKLRSVYFDIS
                     KLDDYGSLSNIDLGKVRVGKTVDLDEYEKDSPMDFTLLKRSTLAELKRGIFYRTRWGS
                     VRPSWHLECAALAIKYLGETVDIHSSGSDFIFPHCENVRALGKALTGRQIANYWIHTE
                     MVMMEGKKMSLSLNNYFTLDDLEKKGYRGKDIRYFLLSSHYRQPLNFSFGALDTARNT
                     VRHLNEFIQKLLRFRTGQGYPDVDQYIYDLKKGFTEAMDDDFGISAALAGLFEFVNKV
                     NPVLSKGFLSEAERDKVLDLMRKIDSVLGVMDFEEERIGEEARSLIAQREGARRRRDY
                     GESDRLRDRLAEMGILVQDTPGGTIWWKK"
     gene            complement(3029..3685)
                     /gene="nth"
                     /locus_tag="P5573_05140"
     CDS             complement(3029..3685)
                     /gene="nth"
                     /locus_tag="P5573_05140"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HRT27477.1"
                     /translation="MNDEDIMLVIRTLKGQLRKWDTPVVTKIAEEERSPFAILIATIL
                     SSRTKDETTAAAAERLFRLASTPGAMSRLTEEEVAKAIYPVGFYRTKARNVVQACKKL
                     EESFSSAVPDNLEDLMTLPGVGRKTANLVIALGFGGPGLCVDTHVHRISNRLGYVKTK
                     TPGETERKLREKLPRRYWSIYNTVMVAHGQNICRPVSPFCGKCPVSRYCDRVGVTRSR
                     "
     gene            complement(3706..>4228)
                     /locus_tag="P5573_05145"
     CDS             complement(3706..>4228)
                     /locus_tag="P5573_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HRT27478.1"
                     /translation="DFREMVRESFYPIYPEEEEVAAEDEEYEEDAFDLISFFVEESLR
                     PILEILADKYGDLIKKSGWSEGYCPVCGKEPKIGEIRDDAGHRVLYCTQCGVEWDFTS
                     IKCPFCGNEEQKTLAYFTVEGDERYRVEVCDDCKRYIKTVDFRDTKEKADLEVEDIAT
                     IHLDMLANEEGYH"
BASE COUNT          983 a         1163 c         1230 g          852 t
ORIGIN      
        1 gcgactcctg cgtcccgaac gcagtgccct accaagctgg gccacatccc gactgtgctg
       61 attaatataa aatttgcggc aaaaagtcca ttatttttat cgtccgttcc tccgcttctt
      121 cctctggagc gaaggcggca gttgttgaat tctcggcaga gaggtggtat aaactaacaa
      181 actcttgtaa atacagaggg gtatcttttg tgatcatcta cgacctaaaa tgtgaaaaaa
      241 accaccgatt cgaaggatgg ttcgatgaca gggctgcctt cgaggcgcag aaatcgggga
      301 aattcatatc ctgtccggtt tgcggcagca cggatatcga aataatccct tccacggtcg
      361 ccgtcctcgg aaaggattct agaaatgacg cgggtgacag gagagaggtc tatccgttga
      421 aagccgccca gctcctccaa gagtacctca acaccaactt cgacgatgtg ggtgacaggt
      481 tcgccgaagt ggcgctgaag atccacaaag gagaagagga gaagcggaac atcaagggga
      541 cgacgaccaa agaggaggaa gagacgctca gggagacagg cgtccagttt ttcaaggtca
      601 ccgttcctaa attggacagt tgaaggacgg agcagcatga cagtatcggg ggttgagata
      661 gtacccccgt tttttttgac ggcctcttat ggaaaagcgc gacagtgaca tcctgggcat
      721 gatcggcaac accccgctgg tggagctccg taaactgaac ttcaataaga gagtcaaggt
      781 tcttgcaaaa ctggagagct tcaaccccgg tgggtcgata aaggaccgcc ctgccctgtt
      841 catgatcgtc aaggccgaag agcgcggcga gctgacgaag ggaaaaacga tcttggaggc
      901 aacaagcgga aacaccggga tcggcctcgc catgatagcc gccgtgaagg attacaaact
      961 gctcctgatg atgcccgaat cggcgagcga agagcggaaa aagatcctca aggccttggg
     1021 cgcggaactc cgcttgacgc cggcggcgct cgggaccgat ggcgccatcg aggcggccta
     1081 caatctcgcc cgtgagcaac ctgacaagta ttttctcgtg gaccagttca acaacgagga
     1141 caacattgcc gcccactatc acggcacggc cgaggaaata tggcgccaga cggaggggaa
     1201 ggtgacgctg gtggtttgcg ccctcggcac ttcggggaca gccatgggga tttccagacg
     1261 tctgaaagag tacaaccccg acgtcaaggt cgtgggggtc gagccttacc tccgtcacaa
     1321 gatacagggt ctgaagaaca tgaaggagtc ctacgtcccc ggcatcttcg acaagaacag
     1381 gctcgacgaa aaggtaaata tcctggacga agatgccttc acgatggcgc gaagactctt
     1441 gcgggaagag ggaatcctgg ccgggatgag ttccggggcc gcggcccatg tcgccctgga
     1501 gaaggcgaag gaaatggacg ggggggttgt cgtcgtgatc ttcccggacg gcgcggaacg
     1561 ctacctgagc acggagctct tcgccgaccg tgagaaaacg accctccgca tctggaacac
     1621 cccaacgaga aagaaagatt tcttcgttcc cgtcgaccct gaggaaatac gcgtgtattc
     1681 ctgcgggccg acagtcgacc tcgtctatca cgtgggaagc taccggcgct tcgtggtctc
     1741 cgacatggtc gtccgctacc tggaatcaaa aggataccgc gtcaagcacg tcatcgattt
     1801 catcgacctt gcagaccggg ccatcaaagg ggcggagcag gcaggcaggg acctgaagga
     1861 ttttaccgaa cactgcttcc aggagtttct caagggcacc gagacattga acatccagaa
     1921 gggggcccta tttccaaagg cgagcgacaa catcgacgag atggtcgacc tcgtcgaaaa
     1981 actgacggaa aaggggtacg cctacgagaa gttgcggagc gtttatttcg acatctccaa
     2041 gctggacgac tacggcagtc tctccaacat agacctgggg aaggtacgcg tcgggaagac
     2101 cgtcgacctc gacgagtacg aaaaggacag ccccatggac tttacactcc tcaagcggtc
     2161 caccctggcc gagcttaagc ggggaatctt ttacaggacc agatggggga gtgtgaggcc
     2221 gagctggcat ctcgagtgcg ccgcccttgc catcaaatac ctcggggaaa cagtggacat
     2281 ccattccagc gggtccgatt ttatttttcc ccattgcgaa aacgtcaggg cgctcggcaa
     2341 ggccctcacg gggaggcaaa tcgccaatta ctggatccac acggagatgg tgatgatgga
     2401 agggaaaaag atgtcccttt ctctgaacaa ctactttaca ctggatgacc tggagaagaa
     2461 gggctaccgg ggaaaagaca tccggtactt cctcttgagc tcccactaca ggcagccgtt
     2521 gaatttttcc ttcggcgccc tggatacggc gaggaacacc gtccggcatc tgaatgaatt
     2581 catccagaag ctgctccggt tcaggacagg tcagggttat ccggacgtcg accagtatat
     2641 ctacgacctc aaaaaaggct tcaccgaggc catggacgac gacttcggca tatcggccgc
     2701 ccttgccgga ctcttcgagt ttgtcaacaa ggtcaacccc gtcctttcga aagggttcct
     2761 gagcgaggcc gaacgggaca aggttctcga cctcatgcga aagatcgact ccgtcctcgg
     2821 ggtgatggat tttgaggagg agaggatcgg cgaggaggcg aggtccctca tcgcgcagcg
     2881 ggaaggggcg agaaggcgga gagactacgg ggagtccgac cggctgcggg accggctggc
     2941 ggagatggga atcctcgtcc aggacacgcc gggcggcact atctggtgga aaaagtaaaa
     3001 cagggctcga tggcgtgaat cttccgcgtc agcgactcct ggtgacaccc acccggtcac
     3061 agtaccgaga gaccggacac ttgccacaga agggcgacac gggacggcag atgttctggc
     3121 cgtgggcgac catgaccgtg ttgtagatag accagtacct ccggggaagt ttctcccgga
     3181 gctttctctc cgtttctccg ggggttttcg tctttacgta acccagccgg ttcgaaatcc
     3241 ggtgcacgtg ggtatccacg caaagcccgg ggccgccgaa gccgagggca atgacgaggt
     3301 tcgccgtttt cctccccacc cccggcaggg tcatgaggtc ttcgagattg tccggtacgg
     3361 cggacgaaaa actctcctca agctttttgc aggcttggac gacgttcctt gccttagtcc
     3421 ggtagaaccc tacgggatag atcgcctttg cgacctcctc ttcggtgagc cggctcatcg
     3481 ccccgggtgt agaggcaagc cggaagaggc gctccgcggc agcagcggtc gtttcgtctt
     3541 tcgtccgcga actcaggatc gtggcgatga ggatcgcgaa agggcttctc tcttcttccg
     3601 caatcttggt gacgacgggc gtatcccact tccgcaactg ccctttgagg gtacggatga
     3661 ccagcatgat gtcttcatca ttcatagaag ggtaaaggtg aagggtcagt ggtatccttc
     3721 ttcgttcgcc agcatgtcga ggtggatcgt cgcgatgtcc tctacctcaa ggtccgcctt
     3781 ttccttcgtg tccctgaagt ccacggtctt gatgtagcgc ttgcaatcgt cgcagacctc
     3841 cacgcggtac cgctcgtcgc cttccacggt gaagtaggcg agcgtcttct gctcctcgtt
     3901 cccacagaag ggacacttga tgctcgtgaa atcccattcg acgccgcatt gcgtgcagta
     3961 caggacccgg tgtcctgcat cgtctctgat ctcgccgatc ttcggctctt tgccgcagac
     4021 cgggcagtat ccctcagacc atcccgattt cttgatcagg tctccgtact tatcggcgag
     4081 gatttccaag atcggccgca ggctttcctc gacgaagaaa gaaatcaggt caaaggcgtc
     4141 ttcttcatac tcttcgtctt ccgccgcaac ctcttcttcc tccggataga tagggtaaaa
     4201 ggattcgcgg accatctccc ggaagtcg
//
LOCUS       DAOTMX010000143         4202 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_17118_length_4202_cov_3.855799, whole genome shotgun sequence.
ACCESSION   DAOTMX010000143 DAOTMX010000000
VERSION     DAOTMX010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4202
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17118_length_4202_cov_3.855799"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="P5573_05150"
     CDS             <1..778
                     /locus_tag="P5573_05150"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HRT27479.1"
                     /translation="MAYLEAIESGQFQFLPERIYAEGFIGAYARELGIDSSLILSHYR
                     DYLRNLTGAGHEKETRKTSPPAPKRPADAARRADIGRPLLRWVKGHRQMLGRSLVVLF
                     AAFAVFYFLYTDERPEPGVVQEAPATIAEKAVPADRVGPGEPAKEPGKEDAGTVAAPG
                     QSGVPAGTEAKPATQREPLNLVVTAKEKTWVRVTADEEPPFDVLLRPGDRIERQAQDV
                     FHIDIGNAGGVDIQFQGKQLDRVGKRGEVVHLVLPEPADR"
     gene            843..1031
                     /locus_tag="P5573_05155"
     CDS             843..1031
                     /locus_tag="P5573_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HRT27480.1"
                     /translation="MFGIGMPELIVILVIVLIIFGAGKLPEIGGALGKGIKNFKKSVH
                     DKDEAGVTPDSGKVDEKK"
     gene            complement(1045..1452)
                     /locus_tag="P5573_05160"
     CDS             complement(1045..1452)
                     /locus_tag="P5573_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRT27481.1"
                     /translation="MVNKVILIGRLGADPEVRFTQDGTMVANLRLATNEYRKDKNGER
                     VERTEWHRVVTFGRLAEVCQSYLTKGKLVYIEGRLQTRSWEDKDGNKRATTEIVAATL
                     QMLDGKGQEKGRDAWAEPTAHPESDQIPDDVPF"
     gene            1627..2376
                     /locus_tag="P5573_05165"
     CDS             1627..2376
                     /locus_tag="P5573_05165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRT27482.1"
                     /translation="MIALLRSAVLVMLAGLWTGLVSSFVILGYPFFPEGENTVHRVAR
                     KWARGILWLSGVEVEVRGTENILPDRPQIFMVNHQSFFDIFIVLAFIPAQFRFIAKKE
                     LFRVPIFGSALRRYGTIEIDRQHRESAVKSIGEAARKIREGKSVVTFPEGTRSPDGEV
                     KDFKKGVFHLAQMAGVPVVPVSIVGSREIMPKHSLKVSPGKIVMVIDKPVETAAYSEE
                     RRDKLIAKVRDVIVKNFCDVKKARPSRASQG"
     gene            2392..>4202
                     /locus_tag="P5573_05170"
     CDS             2392..>4202
                     /locus_tag="P5573_05170"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HRT27483.1"
                     /translation="MANDGKRMNRGSSSSPGRLREVVATFCLALALFLLLCFFSYNPY
                     DPSFSHSPMSGGRVVDNLCGVVGSYSADALIRFLGASAFWLPVFLAVVAVRLFRKEYD
                     AFTLPMVCGFLALVFSTSGLLSIAAGQVRFRGAELSAGGVLGSASVEFLKSYLNTTGT
                     LVFLVLLLIVALMVVADLSLVVLARRFADTVGGIRSRLWSFVLGKMERREEERLEKNV
                     RPPEREAGETAWEEEKDAKPVPIILEEKPLPAAERTKGRKGGQAAFEFIKTKEAFQLP
                     PLTLLDDLPRRDTKVKKEGLIANARLLEQKLADFGVEGRVVEVKPGPLITTYELEPAP
                     GVKLNRITNLSEDLAMALRAPSIRIAPITGKAVVGIEIPNEEREPVHLRDVLDHPAFL
                     ESRFRLPIALGEDIVGMPVVADLARMPHLLIAGTTGSGKSVSLNAMICSILFRSAPDE
                     VKFLMIDPKRLELSGYEGIPHLLHPVVVDPKDAALVLKWAVVEMERRYRVIGESGVKS
                     IEQYNRLVEKEGLTGWRPSPPGKPEAPGEEGEVTDVSSPADADVKSPRERLPYIVVVI
                     DELADLMMVAQRNVEESLTRLAQMARAAGIHLILATQ"
BASE COUNT          843 a         1203 c         1295 g          861 t
ORIGIN      
        1 gatggcctat ctcgaagcga tcgagagcgg tcaattccaa ttccttcccg agcggatata
       61 cgcggagggc tttatcgggg catatgcccg agaactcggt atcgacagct cgttgatcct
      121 ttcccattat agagattacc tgcgtaatct caccggcgcc gggcacgaaa aggagacgag
      181 aaaaacttca ccccctgcgc cgaaaagacc ggccgacgcc gcacggcggg ccgacatcgg
      241 ccgacccctg ctccgatggg tgaaaggcca ccgacagatg ctcggccgct ctcttgtcgt
      301 cctcttcgcc gcttttgccg ttttttattt tttgtacacc gacgagaggc ctgaacccgg
      361 tgttgtgcag gaggcacccg cgacgattgc agaaaaggcg gtgccggcgg accgggtcgg
      421 ccccggggaa cccgcgaagg aacccggaaa ggaggatgca ggcactgtag ccgctcccgg
      481 ccagtccggt gtccccgccg ggaccgaagc gaagcccgcg acgcaacggg agcccctgaa
      541 cctggtcgtc acggcgaaag agaagacatg ggttagggtc acggcggacg aggaaccgcc
      601 tttcgatgtc ctgcttaggc cgggagacag gatagagcgt caggcgcaag atgtctttca
      661 tatcgacatt gggaacgccg gcggagtaga tatccagttc caaggaaaac aactggacag
      721 agtcgggaag cgcggcgaag tagttcacct ggttttgccc gagccggcag accgatgagc
      781 agcgttgcag cggcttatcg atgaattaga ataggacctc actttaagga gaaaatagag
      841 tcatgtttgg gataggaatg ccggagctta tcgtcatcct ggtcatcgtt ctgatcattt
      901 tcggtgcggg caaactaccg gagataggag gagcactcgg caagggcatc aagaacttca
      961 agaaatccgt gcacgataag gacgaggccg gcgtgacgcc ggactccggg aaagtcgacg
     1021 agaaaaaata gcgtcccctt cccctcagaa gggcacgtcg tcgggtatct ggtcggattc
     1081 ggggtgggct gtcggctcgg cccaggcatc ccgtcccttt tcctgacctt tcccgtcgag
     1141 catctgcaag gttgcggcga cgatttccgt cgtcgcgcgt ttgttcccgt ccttgtcttc
     1201 ccacgacctg gtctgcagcc gaccttcgat atagaccagc ttccccttcg tgaggtagct
     1261 ctgacatacc tccgccagcc tgccgaaggt gacgaccctg tgccactctg tccgctcgac
     1321 tcgctcgccg tttttgtcct tgcggtattc attcgtcgcg agcctgagat tggccaccat
     1381 ggtcccgtcc tgcgtaaaac ggacctcggg gtcggcgccg agccgtccga tcagtatcac
     1441 tttattcacc atgcccttcc ctccagtgag tcgttgcggc gggagcccga atcgaagctc
     1501 tgttaagctc atagcccgat tgcgaggcgt tcgtcaagaa gattaaaagg gtgagatgtt
     1561 gacttcgatg ggatgttccg ctacaaatgt gggaaaagtt ttcgatttta cttggcaggt
     1621 ttccggatga ttgccctgtt gcggtccgct gttctggtga tgctggccgg cctgtggacc
     1681 ggccttgtgt cctccttcgt catcctgggg tatccttttt tcccggaggg tgagaatact
     1741 gttcacaggg tcgcgcggaa gtgggcgaga ggcatccttt ggctttcagg agtggaggtg
     1801 gaagtgaggg gtacggaaaa catccttccg gaccgaccgc agatttttat ggtcaaccac
     1861 cagagctttt tcgacatctt catcgtcctc gccttcattc ccgcccagtt ccgctttatc
     1921 gccaagaagg agctcttccg ggtgccgatt ttcgggagtg ccttgagaag atacgggacc
     1981 atcgaaatcg accgccagca tcgcgaaagc gccgtcaaga gtatcgggga ggcggcgagg
     2041 aagatcagag aaggcaaatc ggtggttact ttcccggaag ggacgaggag cccggacggc
     2101 gaggtcaagg atttcaagaa gggcgttttc cacctcgccc agatggccgg cgtccctgtc
     2161 gtccccgttt cgatcgtcgg gagccgggag atcatgccca agcactctct caaggtcagc
     2221 ccggggaaaa tcgtcatggt catcgacaaa ccggtggaga cggcggccta ttccgaggaa
     2281 agaagggata aactcatcgc gaaagtccgg gatgtgatcg tgaaaaattt ttgcgacgtc
     2341 aagaaggctc ggccgtctcg ggcttctcag gggtgagagg caggcttctg aatggcaaac
     2401 gacggtaaaa gaatgaacag gggctcatca tcatctcctg gccgattgcg cgaggtcgtg
     2461 gcgaccttct gtctggccct tgccctgttt cttctcctct gctttttttc ctacaaccct
     2521 tacgaccctt ccttctctca ttctccgatg tcaggcggta gagttgtcga taacctctgc
     2581 ggcgtcgtag ggtcatacag tgccgacgcc ctcatccgct tcctgggggc gagtgccttc
     2641 tggctgcctg tttttttggc ggttgtcgcc gtcaggctct tcaggaaaga gtacgatgcc
     2701 tttaccttgc ccatggtctg cggcttcctc gctctagtct tttcgacttc gggcctcctg
     2761 tctatcgccg ccgggcaggt ccggttccgg ggagcagaat tgtccgccgg cggggtgctg
     2821 ggaagcgcct ctgtcgagtt tttgaaatca tacctgaaca ccacgggtac gttggtcttc
     2881 ctcgttctcc tattgatcgt cgccctcatg gttgtcgccg acctctccct ggtcgtgctg
     2941 gccaggaggt tcgccgacac cgtgggggga atccggtcaa ggctgtggtc ctttgtctta
     3001 ggtaagatgg agcgccggga agaggagcgg ctggaaaaga atgtgcgtcc gccggagcgg
     3061 gaggcggggg aaacagcctg ggaagaagaa aaagacgcaa agcccgtgcc catcatcttg
     3121 gaagaaaaac ccctgccggc ggcggaacgg acaaaaggaa ggaagggcgg gcaggcggcc
     3181 ttcgagttca tcaagacgaa ggaagccttt cagctcccgc cgttgacgct cctagacgac
     3241 ctgccgcgga gggacacgaa ggtcaagaag gagggtctga tcgcgaacgc ccggcttctg
     3301 gagcagaagc tcgccgattt cggcgtggag gggcgggtcg tggaagtgaa accggggccg
     3361 ttgatcacga cgtacgaact ggaaccggct cccggcgtga aactcaacag gattaccaac
     3421 ctgtcggaag acctggcgat ggcgctccgc gcaccgagta tccggatagc cccgataacg
     3481 ggcaaggccg tagtcggcat cgagatcccg aacgaggaac gcgagccggt ccacctgagg
     3541 gacgtcctcg accacccggc cttcctggag tcccggttta ggctccccat cgccctgggc
     3601 gaagacattg tcgggatgcc ggtagttgcc gaccttgcga ggatgcccca tctcctcatc
     3661 gccggcacca ccgggtccgg gaagagcgtc tccctgaacg cgatgatctg cagcatttta
     3721 ttcaggtctg cccccgatga agtcaagttc ctgatgatcg accccaagcg ccttgagctt
     3781 tccggctacg agggcatccc ccacctcctc caccccgtgg tcgtagaccc aaaggacgcc
     3841 gccctcgtcc tcaagtgggc cgtcgtcgag atggagcggc ggtaccgggt tatcggcgaa
     3901 agcggggtga aaagcatcga gcagtacaac aggcttgtcg agaaggaggg cctgacgggc
     3961 tggaggcctt caccgccagg gaagccggaa gcccctggcg aggaggggga ggtaaccgat
     4021 gtctcatccc cggcggacgc ggatgttaag tctccccggg agaggctccc ctacatcgtc
     4081 gtcgtcatcg acgaactcgc cgacctcatg atggtggcgc agaggaacgt cgaagaatcc
     4141 ctgacgcggc tcgcccagat ggcgcgggcg gcgggcatcc atctcatcct ggcaacccag
     4201 ag
//
LOCUS       DAOTMX010000144         4152 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_17361_length_4152_cov_3.585062, whole genome shotgun sequence.
ACCESSION   DAOTMX010000144 DAOTMX010000000
VERSION     DAOTMX010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4152
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17361_length_4152_cov_3.585062"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="P5573_05175"
     CDS             <1..746
                     /locus_tag="P5573_05175"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HRT27484.1"
                     /translation="LSFFALSGEGAEKFPRPVGAVNDFANVIPNAYRQRMDALAREVL
                     AKTGTSIVVATVPTIGDNDTADYANRLYQSWGIGKKGEDKGVLIFLALKERQVRIETG
                     YGVEGILPDGRVGAILDRDVVPFLRKGEYGRGLLNAMLDVAAVVAEDAKASIGEKRVV
                     PQPHRGMEGLPREKGISWAGTLVSLLILVVLFGLLLATPQGRSLLPLILLMFLGGGRG
                     GGGFGAFGGGGFGGFGGGSSGGGGAGRGF"
     gene            762..1493
                     /locus_tag="P5573_05180"
     CDS             762..1493
                     /locus_tag="P5573_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27485.1"
                     /translation="MVKIPEKPEYIFQEFTSDFLSVFGPDLISIILYGSGARNDYVPG
                     KSDINFLIVLSEQGIDNLGSVFGVLPKWRKRRVALPLVMTKAFINSSLDCYPVEFLNM
                     KRCYVLVFGEDVLKDLTFDKPALRLQVERELKGKLLLLRTRYLETEGKSRRVEDLIKE
                     SVTAFIAIFNALLYLRDVEVPLGRGEVVKAVAKAFAAETGTLLQCVDIKEGRSRLAAA
                     EAERVFQGYAKELALLTDLVDKIKV"
     gene            1513..2091
                     /locus_tag="P5573_05185"
     CDS             1513..2091
                     /locus_tag="P5573_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HRT27486.1"
                     /translation="MTPGKKKWIVVLVIVALVAFFTYSFVRGTYNQFVRLDEAVKASW
                     SQVENQLQRRYDLIPNLVETVKGYAAHEKEVLTEVTNARARVGGAGTVDEKIKANNEL
                     TGALSRLLVVVERYPDLKASQNFIRLQDELAGTENRIAVERRRYNEAVQAYNVAIRSF
                     PANLLAGLFGFKAATFFEAPAAAKTAPEVKFR"
     gene            complement(2211..2408)
                     /locus_tag="P5573_05190"
     CDS             complement(2211..2408)
                     /locus_tag="P5573_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HRT27487.1"
                     /translation="MRTVKIEGMSCQHCVKAVTKALEAIPGVTDVKVDLARGEASFEE
                     AQPVDDQTLRERIRKAGYRLG"
     gene            complement(2468..3259)
                     /locus_tag="P5573_05195"
     CDS             complement(2468..3259)
                     /locus_tag="P5573_05195"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF006070.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HRT27488.1"
                     /translation="MKKLSELFSLKGKTALITGGGRGIGKFIATGLAEAGADLIITSR
                     KMKNLEATAEEVSATYGVQVLPVACDMARAEDIESMVKAATEKFPRIDILVNNAGATW
                     GAPTLEFPLDRWDQLFNVNVRGVWILTQKIANLMKDQGGGNIINISSIMAFRGSEELA
                     HPAVPYNSTKAAINLLTMNLAVKLAPYNIRVNAIAPGFFRTDMMTYIEKPEFKKAYDL
                     ILSQIPMRRVGDLDDMRGVAVFLASDASAYMTGHVLVNDGGYLAK"
     gene            complement(3283..>4152)
                     /locus_tag="P5573_05200"
     CDS             complement(3283..>4152)
                     /locus_tag="P5573_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27489.1"
                     /translation="RLGGCLRKAVIIGYERRPTYVKPKDASTVILLRRRPNAVKGGIE
                     VLMVLRHPGSRFVADAYVYPGGAIDEEDCAPAMEVLCRGMTREKAVGLMDDIPPEKAL
                     GAWVAGIRETFEEVGILLAYIKDGGLITLSDPKEAERFSNYRDALGKGADDFRSMLER
                     EGLTLAADRLHYFSHWVTPEPLPIRYDVRFFVAEAPTGQEALHDGHELVGHLWVTPQE
                     ALDAYHRGDFNLVLPTIMTLKELCAFKTVEDVILATKGKGIPRILTKMERKGGRNVEI
                     MPDGSVFAPSPCK"
BASE COUNT          816 a         1157 c         1246 g          933 t
ORIGIN      
        1 gtctttcgtt cttcgcgctc tccggtgagg gggctgaaaa atttccgcga ccggtggggg
       61 ccgtcaatga tttcgcgaac gtaataccca acgcataccg ccagaggatg gatgccctgg
      121 cgagagaggt tctggcgaag accgggacgt ccatcgtcgt ggcgacggtg ccgaccatcg
      181 gagataacga cacggcggat tacgccaaca ggctttacca atcctggggt atcggcaaga
      241 aaggggagga caagggagtc ctaatctttc ttgccctcaa ggaaagacag gtcaggattg
      301 agacgggata cggcgtggag ggaattctcc ccgacggacg cgtgggcgct atcctggacc
      361 gggatgtggt gccctttctc aggaaaggcg aatacggacg gggactcttg aatgccatgc
      421 tcgacgtggc tgccgtcgtt gccgaagatg caaaggcgtc catcggtgaa aaacgcgtcg
      481 tcccgcagcc acaccggggg atggaagggc taccccggga gaaagggatc tcctgggccg
      541 gcaccctcgt ctcgttgctc atcctggtcg tcctcttcgg tctgctcctg gcgactcctc
      601 agggtcggtc tctcctgccc ttgatcctgc tcatgttcct cggcggcggc cgcggcggtg
      661 gggggttcgg ggctttcggc ggcggcggtt tcggtggttt cggcggcggg tcgagcggcg
      721 gcggcggagc cggacggggt ttctgaaaaa aggaggcgta aatggtgaag ataccggaaa
      781 agccggagta catctttcag gaattcacct ccgattttct gtctgttttc ggtcccgacc
      841 tgatttccat aattctttac ggaagcgggg caaggaacga ttatgtgccg gggaaatcgg
      901 atatcaattt tttgatcgtc ctttcggaac agggcatcga caacctgggc agtgttttcg
      961 gcgtcctgcc gaaatggagg aagaggaggg tcgccctgcc cctagtcatg acaaaggcct
     1021 tcatcaactc ttccctcgac tgctaccccg tcgagtttct caacatgaag cgctgttacg
     1081 tgctcgtctt cggcgaggac gttctcaaag atttgacttt cgataaaccg gcgctccggt
     1141 tgcaggtcga gagagagctg aaggggaagc tcctccttct caggacgagg tacctggaaa
     1201 cggagggtaa aagtcgccgg gtcgaagacc ttatcaagga atcggtcacg gctttcatag
     1261 ccattttcaa cgcccttctc tatctaagag acgttgaggt tcccctgggg cggggagagg
     1321 tcgtaaaggc cgtggcgaag gcatttgcgg ccgagacagg gaccttgcta cagtgcgtgg
     1381 atataaagga aggacggagc cggctcgcgg cagcggaggc tgagcgtgtc tttcagggtt
     1441 acgcgaaaga gctggccctg ctgacagacc tggtggataa gataaaagta taagaggaat
     1501 gtggaggtgg agatgacccc ggggaagaaa aagtggatag ttgtactcgt tatcgtggct
     1561 ttggtcgcct tttttaccta ctcttttgtt cgggggacgt acaatcagtt cgtcaggctc
     1621 gacgaagcgg tgaaggcgtc ctggtcacag gtggaaaacc aactccagcg gcgctatgac
     1681 ctcatcccca acctcgtcga gactgtgaag gggtacgccg cccacgagaa agaggttctc
     1741 accgaggtca ccaacgcccg tgccagggta ggcggggcgg ggacggtcga cgaaaagatc
     1801 aaggccaaca acgagttgac aggcgccttg agccgcttgc ttgtcgtcgt cgagcggtac
     1861 ccggacctca aggccagcca gaatttcatc aggctccagg atgagctcgc cggcacggaa
     1921 aaccgcatcg ccgtggaaag aaggcgttac aacgaagcgg tccaggctta caacgtggcg
     1981 atcaggagct tcccggcgaa tctccttgcg ggattgttcg gcttcaaggc ggcgaccttc
     2041 ttcgaagcgc cggcggccgc gaagaccgcc cctgaagtga agttcaggtg agcatagtcg
     2101 gtctgtgtct aaagatcgag gcctttgaag aacgttgcct gatagataac ggtacggcga
     2161 ttcgacagca gattcaaagg cctcgattcg ggaggctgaa gaggtactcc ctatccaagt
     2221 ctatagccgg ccttccggat acgttccctt aaagtctggt cgtcgaccgg ctgcgcttcc
     2281 tcgaagcttg cctctccccg ggccaggtca accttgacgt cggttacgcc cggtatcgcc
     2341 tcgagggcct tagtcactgc cttcacacaa tgttgacacg acatcccttc gatcttcact
     2401 gtcctcatct ttcattctcc cctactgacc gtcgtcagtt cggcatctct cgcatgccct
     2461 tcttacctca ttttgccagg taaccgccgt cgttgaccag gacgtggccg gtcatgtaag
     2521 ccgaggcgtc tgaggcgaga aagacggcga cgcctctcat gtcgtcgagg tcgccgaccc
     2581 tgcgcatcgg gatctggctg agaatcaggt cataggcctt tttgaactcc ggtttctcga
     2641 tgtaagtcat catgtcggtg cggaagaagc cgggagcgat ggcgttcacg cggatgttgt
     2701 agggtgcaag tttcaccgcc aggttcatgg tcagcaggtt gatcgccgct ttagtggaat
     2761 tgtaagggac cgcggggtgg gccagttctt ccgaccccct gaaggccatg atcgacgaga
     2821 tgttgatgat gtttccgccg ccctgatcct tcatgaggtt ggctatcttc tgtgtcagta
     2881 tccagacgcc gcgcacgttg acgttgaaga gctgatccca tctgtcgagg ggaaattcca
     2941 gcgtaggcgc accccaggtg gccccggcgt tgttgacgag gatgtcgatc cgggggaatt
     3001 tttccgtcgc cgccttcacc atgctttcaa tgtcctcggc cctggccatg tcacaggcta
     3061 caggcaggac ttggacaccg taagttgcgg agacctcctc ggccgtcgct tcgaggtttt
     3121 tcatcttccg tgaagtgatg atcaggtcgg cgcccgcctc ggcgagtccc gttgcgataa
     3181 acttgcctat tccccttcct cctccggtga taagtgccgt ttttcctttc agactgaaca
     3241 actcgctaag ttttttcatc taaacctcct catacgcctt ttttacttgc atggggatgg
     3301 ggcgaagacg gagccgtcgg gcatgatttc cacgtttctt cccccttttc gctccatttt
     3361 tgtcagtatt cgggggatgc ctttcccctt cgtcgcaagg ataacgtcct cgacggtctt
     3421 aaaggcacag agttccttaa gggtcatgat cgtcgggaga acgagattga agtcgccgcg
     3481 gtgataggcg tcgagggcct cctgcggcgt gacccagaga tgtccgacca gttcatgacc
     3541 gtcgtggagc gcttcctgcc ccgtcggtgc ctcggccacg aaaaacctga cgtcatacct
     3601 gatcgggagc ggttccggtg tgacccagtg ggaaaagtaa tggaggcggt cggctgcgag
     3661 ggtgagccct tctctttcaa gcatcgacct gaaatcgtcg gcccctttgc ccagggcgtc
     3721 tcggtagtta gagaaccttt cggcctcctt tgggtcgctt aaagtgatca acccaccgtc
     3781 cttgatgtag gcaagcaata tgccgacctc ctcgaaggtc tcgcggatac cggccaccca
     3841 tgccccaagg gccttctccg gcgggatgtc gtccatcagg ccgaccgctt tctcccgggt
     3901 cattccccgg cagaggactt ccatggccgg ggcgcaatct tcctcatcga tggcgccgcc
     3961 ggggtatacg taggcatcgg ctacgaacct gcttcccggg tgtcggagca ccatcaggac
     4021 ttcgatgccg cccttcacag cattcggccg cctgcggagc aagatgacgg tcgaggcgtc
     4081 cttcggcttc acgtaagtcg gtcttctctc ataacctata atcacggctt tcctcaaaca
     4141 tcctccgagc cg
//
LOCUS       DAOTMX010000145         4145 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_17385_length_4145_cov_4.103178, whole genome shotgun sequence.
ACCESSION   DAOTMX010000145 DAOTMX010000000
VERSION     DAOTMX010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4145
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17385_length_4145_cov_4.103178"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="P5573_05205"
     CDS             <1..449
                     /locus_tag="P5573_05205"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HRT27490.1"
                     /translation="RLLSLRSRSKKEMESRLRDKGFDESIVGKVIDNLSGLRYLDDDA
                     FAREWARSLAVNRLYGNRRIERSLGEKGIPRETIKRAIAGVREEFSEEEALATLLKKK
                     LKSRPVAELDRKEKNRLARSLMGKGLPAGKIFEVLRNKEEEIDDGE"
     gene            436..3288
                     /gene="alaS"
                     /locus_tag="P5573_05210"
     CDS             436..3288
                     /gene="alaS"
                     /locus_tag="P5573_05210"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HRT27491.1"
                     /translation="MTGSEIRKQFLKFFEDRGHTIVPSSGLIPKDDPTLLFTNAGMVQ
                     FKNLFLGLEDRGYTRAASSQKCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK
                     KESIAWGWEFLTVTMGLPKDRMWVTIYEDDDEAFEIWNKQVGVPAERIVRMGMDSNFW
                     MMGETGPCGPCSEIIYDQGPEVGCGRPECSVFCECDRHLELWNHVFTQFDRDEKGNLN
                     PLPRPNIDTGMGLERLAAVIQGVKSNYECDLLFPIIRFVEKMSGKRYGESENDNVSMR
                     VIADHSRAVTFLIGDGVLPSNEGRGYVLRRILRRACRHGKLLGFDKPFLHDVVIAVVD
                     VMKDAYPDIAEKQSFIQKVIFNEEQRFLETLDTGLRILKDEVDDLKKAGKTVIPGDVV
                     FKLYDTYGFPVDLTADIVRKDNFTVDMEGFEKLMEEQRERARESWKGSGETAVADSYK
                     KLALKGVRSEMVGYEEVTEATSFVTAILKKDEEVEEVGEGENAEIFTDQTPFYGETGG
                     QVGDTGVIEGDDFLFEVWDTQRPDEHLITHIGKVRKGRIRRNDTVRLKVEVKTRRAIE
                     ANHSGTHLLNAALRNVLGDHVKQAGSLVTPDRLRFDFTHFSKIEEAELDRIEMLVNEY
                     IQANVPVKTSILAKEEAFKTGAQAVFDEKYADKVRLVRMGDISAELCGGTHVTRTGNI
                     GLFKIVSESAVAAGVRRIEAVTGLAAVKYVKKLDRELHSVANLLRASTAEVVDRVEKM
                     IQHQKELEREIEALKGKLAAKESGDIVKKVRSLDGLNVLATTVEVSDVKALRDFGDKL
                     RDKMQSGIILLGSKVGDKAMLLCMVTKDLTARYDAGRIVKEIAPIVGGSGGGRPDMAQ
                     AGGTRPENLEKALDRLIEMLGGQKESEKKTTSKRPKAAPAEPVVAGPKSRKAAEAKQQ
                     KPLKKKDAGAKTAQAKPTDKKTKPKGTKSGNSKRRG"
     gene            complement(3549..>4145)
                     /locus_tag="P5573_05215"
     CDS             complement(3549..>4145)
                     /locus_tag="P5573_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27492.1"
                     /translation="SSVLFVYFVGIGLWIAYLFSGLYGIAVAGVGIMGTFYLDVAVSD
                     AGGDRPSEAGKFILVVSSISTFAILAAFSAVVRSYAGEDDLLIDFTRILTVAGLVLGG
                     LTARLMPMLGERFNRPGHEVLRLALAVAVPCAVGVALGPEPLVAFTAGLAVVGTFNGL
                     TGRIEEDQAISDMGIIRLTALFALAFAPVINSLYESLR"
BASE COUNT         1060 a         1128 c         1282 g          675 t
ORIGIN      
        1 tgcgcctcct gtccctccga agccgcagca agaaagagat ggagtcccga ctccgggaca
       61 aggggttcga cgaatccatc gtcgggaagg tgatcgataa tctttctggc ctgcgatacc
      121 tcgacgacga cgccttcgcc cgagaatggg cccggagcct cgccgtaaac aggctttacg
      181 ggaaccggcg tatcgaaagg agtctcggcg aaaaggggat cccccgcgaa acaatcaaga
      241 gggccatagc cggggtcagg gaagagtttt cggaagagga ggcactggca acgctcctga
      301 agaaaaagtt gaaaagccgc cccgtcgcgg agctcgaccg gaaggagaag aaccgtctgg
      361 cccggagtct gatggggaaa ggcctgcctg cggggaagat attcgaagtc ttaagaaaca
      421 aggaggaaga gattgatgac ggggagtgag atcagaaaac agtttttaaa attcttcgaa
      481 gaccggggac acaccatagt cccgagctcg gggttgattc ccaaggacga ccctacgctc
      541 cttttcacca acgcgggcat ggtgcagttc aagaaccttt tcctcggcct ggaggaccgc
      601 ggttacacga gggcggcgag ttcccagaag tgcgtccgcg ccggcggtaa gcacaacgac
      661 ctggaaaacg tcggctacac ggcccgtcac cacacctttt tcgagatgct gggaaacttc
      721 tctttcggag attacttcaa gaaggaatcc atcgcctggg gatgggagtt cctgaccgta
      781 accatggggc tgcccaagga caggatgtgg gtcactatat acgaggatga cgacgaggcc
      841 ttcgagatct ggaacaagca ggtgggtgtt cccgccgagc ggatcgtgcg catgggaatg
      901 gacagcaatt tctggatgat gggggaaacc ggaccctgcg ggccgtgctc ggaaatcata
      961 tacgaccagg ggccagaggt cggttgcggc cggccggaat gcagcgtgtt ttgcgaatgc
     1021 gaccgccacc tcgagctctg gaatcacgtc ttcacccagt tcgaccgcga cgaaaaggga
     1081 aacctcaacc ccctccctag gccgaacatc gacacgggta tgggcctcga gcgcctcgcc
     1141 gccgtcatcc aaggcgtcaa gagcaattac gaatgtgacc tgcttttccc gatcatccgg
     1201 ttcgtcgaaa agatgagcgg caagcggtac ggggagagcg aaaacgacaa cgtgtccatg
     1261 agggtcatcg ccgaccacag ccgggccgtg acctttctca tcggtgacgg ggtgcttccc
     1321 agcaacgaag ggcgcggtta cgtcctgcgc cgaatattga ggcgcgcctg ccgccacgga
     1381 aagctcctgg gcttcgataa accattcctg cacgacgtcg tgattgccgt cgtcgacgtg
     1441 atgaaggacg cctatcccga catcgcggaa aaacagtcct tcatccagaa ggtcatcttc
     1501 aacgaggaac agcgtttcct cgagaccctc gacacaggac tgcgcatcct caaggatgag
     1561 gtcgacgacc tcaagaaggc cggaaagacc gtcatccccg gggacgtcgt attcaagctc
     1621 tacgacacct acggcttccc cgtcgacctc acggcggaca tcgtcaggaa agacaacttt
     1681 acggtcgaca tggaaggctt cgaaaagctg atggaagaac agagggaaag ggcccgtgaa
     1741 tcctggaagg ggagcggcga gacggcggtc gccgacagtt acaagaaact cgccctcaag
     1801 ggtgtccgtt ccgagatggt gggctacgag gaggtgacgg aggccacctc gttcgttacg
     1861 gccatcctca agaaggacga ggaggtggag gaggtcgggg aaggcgaaaa cgccgagata
     1921 ttcaccgacc agaccccctt ctacggggag acgggcggcc aggtcgggga cacgggggtc
     1981 atcgaaggcg acgattttct gttcgaggtc tgggatactc agcggcccga cgagcacctc
     2041 atcacccaca tcggtaaggt caggaagggc aggatccgca gaaacgacac ggtgcgtctt
     2101 aaggtggagg tgaaaacgcg acgggccatc gaggccaacc attcgggcac gcaccttctc
     2161 aacgccgcgt tgcgcaacgt cctgggcgat cacgtcaaac aggccggttc gctcgtgacg
     2221 cccgaccgtc ttcgtttcga tttcacccat ttttccaaga tagaagaggc cgagctcgac
     2281 aggatcgaga tgctcgtgaa tgaatacatc caggccaacg ttcccgtgaa aacgagcatc
     2341 ctggccaaag aagaggcctt caagaccggc gcccaggccg tcttcgacga aaaatacgcc
     2401 gacaaggtcc gactggtcag gatgggcgac atcagcgctg agctctgcgg cggcactcac
     2461 gtcacgcgga cgggaaatat cggcctcttc aagatcgtct ccgagtcggc cgtcgcggcg
     2521 ggggtgcgac ggatcgaggc ggtcacgggg ctcgccgcgg tcaagtacgt caagaaactc
     2581 gaccgcgagc tccacagcgt agcaaacctc ctgagggcta gtacagccga ggtagtcgac
     2641 cgggtcgaga agatgatcca gcatcagaag gaactcgagc gggaaatcga agccctgaaa
     2701 ggaaaactcg ccgccaagga gtcgggcgac atcgtcaaga aagtaagatc gctcgacggg
     2761 ctcaacgttt tggcgacgac cgtggaggtc tccgacgtca aggccctgcg tgacttcggc
     2821 gacaagctga gggacaagat gcagtcgggc atcatccttt taggcagcaa ggtgggggac
     2881 aaggcgatgc tcctttgtat ggtcacaaaa gacctgacgg cccggtacga cgccggcagg
     2941 atcgtaaagg agatcgcgcc gatcgtcggt ggaagcggcg gcggccgtcc tgacatggcc
     3001 caggccggag gaacgaggcc ggaaaacctc gaaaaggcct tagacaggct catcgagatg
     3061 ctgggaggtc agaaagagtc ggagaagaag acgacatcta aacggcctaa agcagctccc
     3121 gctgagccag tcgtcgccgg gccgaaatca cggaaggcag cagaggccaa gcagcaaaag
     3181 cctctgaaaa aaaaggatgc gggagcgaag accgctcagg ccaaaccgac tgataaaaaa
     3241 accaagccta agggaacgaa gtccggcaat tcaaaaagaa ggggttgaat accgagcaga
     3301 acaacggatg tgaatcgcaa cacgatagaa actctggaag aactcgagaa aaatctcagg
     3361 gatatatata ccgacttgag tagcgatgcc atcccatgtg ttcgccgtgt tggtgatcgg
     3421 cgcgtcgcat gaaacgcgtc gacctcgttc gggagattga agaggtgggt tgcatcttga
     3481 tacggcacgg tgggagagat gataaaattg aagaggaaag aggtggcatt tacaaggtag
     3541 cgaaaatctt aacggaggga ttcgtagaga ctgttgataa caggcgcaaa ggcgagcgcg
     3601 aagagggctg tcaaccggat gatgcccatg tcagaaatcg cctgatcttc ctctattctg
     3661 cccgtcaggc cgttaaatgt ccctaccacc gccaggccgg cggtgaacgc cacaaggggt
     3721 tccgggccga gcgccacgcc cacggcgcag gggacggcga cagcaagggc gagccggagt
     3781 acctcgtgtc ccggcctatt gaaccgttct cccagcatag gcataagtcg agccgtcaat
     3841 cctcctaaca ccaggcccgc aaccgtaagg attcgagtga agtcgatgag gaggtcatcc
     3901 tcgccggcat agctccttac tacggcagag aaagcggcaa ggatggcaaa ggtggaaatc
     3961 gaggaaacga cgaggatgaa cttcccggcc tcggaaggcc tgtctcctcc ggcatccgat
     4021 acagctacgt cgagataaaa cgtccccata atgccgaccc cggcgaccgc tatcccgtaa
     4081 agcccggaga acagataggc aatccacagg ccaatgccga caaaatagac gaagagtacg
     4141 gaaga
//
LOCUS       DAOTMX010000146         4136 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_17431_length_4136_cov_3.765499, whole genome shotgun sequence.
ACCESSION   DAOTMX010000146 DAOTMX010000000
VERSION     DAOTMX010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17431_length_4136_cov_3.765499"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..924)
                     /locus_tag="P5573_05220"
     CDS             complement(<1..924)
                     /locus_tag="P5573_05220"
                     /inference="COORDINATES: protein motif:HMM:NF019283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secretin and TonB N-terminal domain-containing
                     protein"
                     /protein_id="HRT27493.1"
                     /translation="MKILMAGISALIIMVAAACVVSAEDTGATAAKPGSVAGLPKEEA
                     KYTGQKVSLDFQEANIKSVFRLLSEVSGVNIVAGEDIKGNITVHMKNVPWDQALETIL
                     DTNGLGMKRTGNVISVFTREKMQKAVEERLKEDVAQGKRPQVLIEAKILETTASFARK
                     LGVQWGTGFQTGNVWTGQSARILPSGLTPLTRNLGVRRGTYAVNETSPYSGGTYGVNL
                     ADFTKTGIFPSLGTATVSPTFGLIVGTAKNFVDLDISAYESMGELKVLSAPKVVTFDG
                     TAAYITQGSEIPSVFTDKEGNRSIQWKEATLK"
     gene            complement(927..1349)
                     /locus_tag="P5573_05225"
     CDS             complement(927..1349)
                     /locus_tag="P5573_05225"
                     /inference="COORDINATES: protein motif:HMM:NF016254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HRT27494.1"
                     /translation="MVVLYGAAVSAAEISAQKTGTFPYEKRGKVDPFKPLIRKEAAVQ
                     KEQSAFLSPLQRYNIEELKLVGIMSSEKKRVAIVEDPKGKSYVISKGTLVGRNNGMVA
                     GVLDDRIIVEERTSGNGKEAKTKQIILNLHRVEPEGRL"
     gene            complement(1387..2049)
                     /gene="pilO"
                     /locus_tag="P5573_05230"
     CDS             complement(1387..2049)
                     /gene="pilO"
                     /locus_tag="P5573_05230"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HRT27495.1"
                     /translation="MAITLDDIKKMPTKTKALILAFIFILLGYFYYFFLFQPLYEKKV
                     GLDTKLAGLKQQIEAKQLIVREIQKGKKELATMRESLELALTKLPERKEIPGLLASLA
                     KAGREAGLDFVHFEPLPPPKPTEAKADTKEKSTDKKTVAQAAAGTEKFYDEIPIKVVI
                     SGGFHDTVRFLESVAKLPRIVNVTDITMEGSKDDKEEGRLLTTCMMKTYVFVEKAQAG
                     KK"
     gene            complement(2053..2598)
                     /locus_tag="P5573_05235"
     CDS             complement(2053..2598)
                     /locus_tag="P5573_05235"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HRT27496.1"
                     /translation="MIKVNLLPYRERKKKDLWKRQILVAAATFGAFFLVIGALHGYMY
                     LSVSGLERDVNAAEGRLKELNKLAAEFSSVRADKDLLTKKIEVINNLEESRMDAVLLL
                     NEVARTLPAGQVWLVLLAETGSNLRIDGIARDNLAVAVFMNNLERSPIIKSVDLSASK
                     QDKIANTKVQKFVLNCSLKKG"
     gene            complement(2595..3677)
                     /gene="pilM"
                     /locus_tag="P5573_05240"
     CDS             complement(2595..3677)
                     /gene="pilM"
                     /locus_tag="P5573_05240"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HRT27497.1"
                     /translation="MLNLNLRGLLPDKKHSVGLDIGSSSLKLVEILDSPEGYVLKSYS
                     QLPLERRVIEAGDILNGDVLCGKIRQLFKSAGCRSKKVVVSLSGHKVIARRASFSKME
                     EEELRQLINDEAANYLPFDDVKDFSFDFQILGDSEGNPGQMDVVIVAARKNVVQSYVD
                     VLEKAGLTPVIMDVDSFALETMYEVNYDVREGDIVVLVNIGASMTNINVLKEGCSIFT
                     RDISMGGNDITESIRDRLGVTLDEAEKLKIESSRTDSQAGSPRLDPLEHAEPLFAEIE
                     RSIDYFRSTHGGEYIKEIILSGGSAKTAGIVDALTQQLNIETRIVRPFQNIVCDEKTF
                     RPSYLEEIGPVAAVGVGLGLRRIDDR"
     gene            complement(3753..4019)
                     /locus_tag="P5573_05245"
     CDS             complement(3753..4019)
                     /locus_tag="P5573_05245"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HRT27498.1"
                     /translation="MKVGRFVIVFLLLMGFLIAFGDNGLVDSYVMKEKLKILKDENNQ
                     TMKENDGLKKEVVLLRDNPRYIEMVARKELGMVKKGDVVYRHVE"
BASE COUNT          900 a         1175 c         1003 g         1058 t
ORIGIN      
        1 cttgagagtc gcttctttcc actggatcga gcggttgccc tccttgtcgg taaatacaga
       61 aggtatctca gacccctggg tgatgtacgc agcggtgccg tcgaaggtga ctactttggg
      121 cgccgaaaga accttcagct cgcccatgct ctcgtaggcg gagatgtcca ggtcgacaaa
      181 gttcttcgca gtgccgacga tcagcccgaa ggtgggagaa accgtcgccg taccgagtga
      241 aggaaatata cctgtttttg taaaatccgc taggttcacg ccataggtcc ctccggaata
      301 aggagatgtc tcgttcacgg cataggtacc cctgcggacg ccgaggttac gagtcagagg
      361 cgtgagcccc gaggggagga tgcgggcgga ttgccccgtc catacgttgc cagtctgaaa
      421 accggtgccc cattgaacgc cgagcttcct ggcaaaagaa gcggtcgttt cgagaatctt
      481 cgcttcgatg aggacctggg gtcttttccc ttgcgccacg tcctctttca acctctcctc
      541 gacggccttc tgcatcttct ctcgggtaaa aacactgatg acgttgcccg tgcgcttcat
      601 gccgaggccg ttggtgtcga ggatagtctc caaggcctgg tcccagggta cgtttttcat
      661 gtggacggtg atgttcccct ttatatcctc gccggcgact atgtttacac cgctcacctc
      721 ggagaggagc ctgaagacgc tcttgatgtt ggcttcctga aagtcgagcg ataccttctg
      781 gcccgtatat ttggcctcct ccttaggcag gccggcgact gaacccggtt tcgccgccgt
      841 tgcgccggta tcctcagccg aaacgacgca ggcagcggcc accataatga tcaatgccga
      901 gatgcccgcc ataagaatct tcattttcat aatcttccct caggttctac gcgatgaagg
      961 ttcagaatta tttgctttgt cttggcttct ttgccgttcc cgctcgttct ttcttcgacg
     1021 ataatccggt cgtcaaggac cccggctacc atgccgttgt tccggccgac caaagtcccc
     1081 ttcgagatga cgtaactctt acccttcggg tcttctacta tggcaacccg cttcttttcg
     1141 ctggacatga taccgacgag tttcagttct tcgatgttat accgctgcaa gggcgacagg
     1201 aacgctgact gttccttctg aacggccgct tcctttctta tcaagggctt gaaagggtcg
     1261 accttcccgc gcttctcata aggaaaggta ccagttttct gcgccgaaat ctccgccgcc
     1321 gacaccgccg cgccgtataa gacaaccaat acgacgaaaa ggtgaccgaa aaacgcagca
     1381 atccgcttat ttcttgccgg cttgggcctt ttccacgaag acataggttt tcatcatgca
     1441 ggttgttaaa agtcttccct cttccttgtc gtctttggat ccttccatgg ttatgtcagt
     1501 cacattgacg atacggggaa gcttggccac gctttccaag aatctcacgg tatcatgaaa
     1561 cccgccgctg atgactacct tgatagggat ttcatcgtaa aatttctccg tccccgcagc
     1621 agcctgggcc accgttttct tgtctgtcga tttttctttc gtgtcggcct tggcctccgt
     1681 gggcttcggg ggcgggagcg gctcgaagtg gacaaaatcg agaccagcct cgcgaccggc
     1741 cttggccaaa gatgccagga ggcccgggat ttctttacgc tcgggaagtt tggtcagggc
     1801 aagctccaga ctttcccgca tagtggcgag ttcttttttg cccttttgga tttctctcac
     1861 gatgagttgc ttggcttcta tctgctgttt aaggcccgca agcttggtgt cgaggcccac
     1921 cttcttctcg taaagcggct ggaaaaggaa aaaataataa aagtaaccca ggaggatgaa
     1981 gataaacgca aggatcaatg cctttgtttt tgtcggcatc ttcttgatat cgtccagggt
     2041 tattgccata cctcacccct tcttgagact gcaattgagg acgaatttct ggaccttcgt
     2101 attggctatc ttatcctgct tcgaggcact caggtcgacc gacttgatga tgggggaacg
     2161 ttccaggttg ttcataaaaa cggcaacagc cagattgtcc cgggctatgc cgtcgatgcg
     2221 caggttggaa cccgtctccg ccaacaaaac cagccagacc tgaccggccg gaagggtccg
     2281 cgcaacctcg ttgaggagca aaacggcatc catcctgctc tcctcaaggt tgttgatgac
     2341 ctcgatcttt ttcgtgagta gatctttgtc tgccctgacg gagctgaact cagccgccag
     2401 cttattcaat tctttcagac gtccttcggc ggcattgacg tccctttcca atccgctcac
     2461 actcaaatac atgtaaccat ggagggcacc aatgacgaga aagaaggccc cgaatgtcgc
     2521 cgctgcaaca agaatctgtc tcttccagag atctttcttc tttcgttctc tgtaaggaag
     2581 aagattgacc ttgatcatcg gtcgtcaatt ctccggaggc cgagaccgac tcccaccgcc
     2641 gcgaccggcc ctatttcttc aaggtaggag ggacgaaacg ttttttcatc gcagacgatg
     2701 ttctgaaaag gccgaactat ccgcgtctcg atattcaact gctgagtgag ggcatcgact
     2761 atcccggctg ttttagccga gccaccgctg aggattatct ccttgatgta ttcgccgccg
     2821 tgagtcgacc ggaagtaatc gatcgacctc tctatctcgg caaaaagagg ctctgcgtgc
     2881 tccaacgggt ctaaacgcgg gctgccggcc tgcgagtcag tcctcgagct ttctatctta
     2941 agcttctcgg cctcgtcgag cgtcacgccc aatctgtcac ggatgctctc cgtgatgtca
     3001 ttgccgccca tggaaatatc gcgggtaaag atcgagcatc cttccttgag aacgttgata
     3061 ttcgtcatgc tcgccccgat attgaccaag acgacgatgt cgccttccct aacgtcgtaa
     3121 ttcacctcgt acatagtttc gagggcgaag gagtccacat ccatgatcac gggcgtcagc
     3181 ccggccttct ccaggacatc cacatagctc tgaacgacat tcttcctggc agccacaatc
     3241 actacgtcca tctgccccgg gtttccctcg ctgtcgccga gtatctgaaa atcaaagcta
     3301 aaatctttga cgtcgtcgaa aggaaggtag ttggctgcct catcattgat cagttgacgt
     3361 aactcttcct cctccatctt cgagaaggag gcccttctgg cgatgacctt gtggcccgac
     3421 agggagacga caaccttttt ggacctgcag cccgcgctct tgaaaagctg acggatcttc
     3481 ccgcaaagga cgtcgccgtt caggatatct cccgcttcga tgacccttct ttccagggga
     3541 agctgggaat aactcttaag gacgtatccc tccggagagt ccagtatctc gaccagcttg
     3601 agcgagctcg acccgatgtc aagacccacc gagtgtttct tgtcaggcaa cagacccctc
     3661 aagtttagat taagcatcgt acctcagtca tctgttgtca acgattagga cctccttacc
     3721 atgaaagtac cgggacgatc tacccccgag gctcattcga catgccggta aacgacgtct
     3781 ccttttttta ccatgcccaa ctctttccgc gccaccatct cgatgtagcg agggttatcc
     3841 cgcaaaagga cgacttcctt tttcagtccg tcgttctctt tcatggtctg gttgttttcg
     3901 tccttcaata tcttcagctt ttccttcatc acataactat ccacgagacc gttatcgccg
     3961 aaggcgatca aaaatcccat aaggagaaga aagactatga cgaatcttcc gaccttcatc
     4021 gccaccctcg tggaaaacgc aaaaacggga agtaaaatgc atatataata aaaaaaggtg
     4081 tgcaacaacc aaaacttttg acaccctcga attcgtgaga tcggttgttt tttaat
//
LOCUS       DAOTMX010000147         4121 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_17492_length_4121_cov_3.548205, whole genome shotgun sequence.
ACCESSION   DAOTMX010000147 DAOTMX010000000
VERSION     DAOTMX010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17492_length_4121_cov_3.548205"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            369..3041
                     /locus_tag="P5573_05250"
     CDS             369..3041
                     /locus_tag="P5573_05250"
                     /inference="COORDINATES: protein motif:HMM:NF015644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HRT27499.1"
                     /translation="MVAFVVLLGLVIFFWRISGVIADWYWFQEVGYENVFMITLWAQT
                     AAALLFGLAFFVVFYANLYIALRLSSRIHLVREDVIHIPPVQIANRPLNILILTASLF
                     FSALAALSGADQWENILRFLNGSPFGVRDPIFQKDIGFYVFKLPFLNHLYGWVMMVLI
                     VTSVAVGLLYFIRRAFLFMPPRTWQVAPQARAHLAVLAALLFFTGIFGSWLSLNELLF
                     VKRGVVFGPGYTDVTTQLWVIKGVMALSFLCGAAFLGFAFRLSWRFPAAAVLLLVALV
                     IVGRGVYPALVQKFKVVPNEIVLERPYLERNIQFTRLAYRLDDIEDQEFPAQESLNRE
                     DLRRNDLTIKNIRLWDHAPLLQTYGQLQEIRTYYKFVDVDNDRYRIGGEYRQVMLSPR
                     ELSYQALPSRSWINEHIIYTHGYGAVMGPVNRITREGLPEFFIKDIPPVSLTDIKVTR
                     PEIYYGEIGNEYVFVKTKRPEFDYPVGEKNVYSRYEGRGGVPLSFLKKLIFVARFGSL
                     TILLSDDITDESRVMYYRRVKDRVSKIAPFATLDGDPYLVISPEGRLLWFLDGYTTTG
                     RFPYSEPTPGLGNYIRNSLKAIVDAYDGTVQLYVSDETDPIIRTYRNIFPGVFKKLEE
                     MPAELRAHVRYPPGMLSVQARMYKAYHMQDPQVFYNKEDLWAVPLKHGSNQEMEPYYT
                     IMKLPGEKKEEFILLLPFTPSRKDNMSAWMAARCDMPNYGKVIVYNFPKQKLVYGPRQ
                     IEARIDQDTEISKQLSLWSQRGSQVIRGSLLAIPIEKSILYVEPLYLAAEKGQLPELK
                     RVIVAFGNAIAMEENLELSLQRIFGGEIPREKGLERVAAGPAEREKTDRQLALEALSH
                     YRKAQEYLRQGNWGAYGEELRRMDDILKSIESRR"
     gene            complement(3047..3901)
                     /locus_tag="P5573_05255"
     CDS             complement(3047..3901)
                     /locus_tag="P5573_05255"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HRT27500.1"
                     /translation="MEKKERKTGMIVAYYPGCSLEGVAREYDRSARLVCDRLGFGLQE
                     VPDWNCCGATAAHSLNHELTRGLSARNLAIVERMNLATVTTPCPGCSIRLKGAAMEAK
                     KESGAPEVMHLLQLLTEAAGTEKIASSVIKPLGGLRLAAYYGCLVTRPRAVARFDDPE
                     QPRSMDRILQALGAETVDWSHKAECCGGAFAASETSIVVDLGGQVLEAARQAGAEALA
                     VACPMCQVNLDTRQWEVESTRGVRYDLPVFYFTQLMGLAYGFTNRAMGLKRLLVNPLP
                     LLKRKGIL"
     gene            complement(3871..>4121)
                     /locus_tag="P5573_05260"
     CDS             complement(3871..>4121)
                     /locus_tag="P5573_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27501.1"
                     /translation="FRPAVALFYELMCSCVERRGRVSEAPLMLRFNILTRQYLKDLTL
                     GIRMFLKGKLPLTAGKVKKMDEVRVLFASWKRRSVKRG"
BASE COUNT          870 a         1161 c         1202 g          888 t
ORIGIN      
        1 gaagatgagc gcgggcgagg caaaccattt tgatatggag tacctgttca tgtcggcgaa
       61 gctatcatcc agccggacga tggtcttcat ttaccttttc acgaaacgat aaatcaagga
      121 taaaatcagc ctttcctttt tcttgacagg atcgatgatt ctccagtagg ttgaccgctg
      181 agcgatgaaa ggatgcagaa cgccgccggc agtataagtt ccagtgccgg gcgtcagagc
      241 aggaaaagac agccgtgtct gtacgctaag cggagagagg atgcgcgctg tgtcctgaag
      301 ttcgggaggg atcgatgaga tttgctgtaa ggccgcctcg gatgccgaga gtaccgagag
      361 gaaagggaat ggttgctttt gtagttcttc tcggactcgt catctttttc tggcggattt
      421 caggagtaat cgccgattgg tactggtttc aggaggtcgg ttatgaaaat gtcttcatga
      481 tcaccctctg ggcgcagacg gcggcggcac tccttttcgg cctggctttc ttcgtcgttt
      541 tctatgctaa cctatatatc gccctgcgcc tgtcctcgag gatacatctg gtccgtgaag
      601 atgtcatcca tatcccgccg gtccagatcg ccaaccggcc gctcaacata ctgattctta
      661 ccgcctccct gttcttcagc gccttggccg ccctcagcgg ggccgatcaa tgggaaaata
      721 tcctcagatt cctgaatgga tcgccgttcg gtgtccggga ccccattttt cagaaggaca
      781 tcggtttcta tgtcttcaaa ctgccctttt tgaatcatct ctatggctgg gtgatgatgg
      841 tcctgatcgt gacgtcggtc gccgtcgggc tcctctactt tatcaggcgg gcctttctct
      901 tcatgcctcc ccggacttgg caggtggcac cccaagccag ggcgcatctc gctgtgcttg
      961 cggcccttct tttcttcact ggtattttcg ggagctggct ttcgctgaac gaactcctct
     1021 tcgtcaaaag gggcgtggtc ttcggcccgg gatacacgga cgtgacaacg cagctctggg
     1081 tcatcaaggg agtgatggcg ctctcctttt tgtgtggcgc ggcctttctg gggttcgcct
     1141 ttcgcctgag ctggagattt ccggccgcgg cggtgctgct cctggtcgcc ctcgtcatcg
     1201 tcggacgggg cgtctatccc gcactcgtgc aaaaattcaa ggtggttccg aatgaaatcg
     1261 tccttgaaag gccgtacctg gagcggaata tccagtttac gcgcttggcc taccgactgg
     1321 acgacataga ggatcaggaa ttccccgcac aggagagctt aaacagggag gatttgaggc
     1381 gcaacgacct cacgatcaag aatatccgcc tttgggacca tgcgcctctg ctacagactt
     1441 acggccagct tcaggagatc cggacttatt acaagtttgt ggacgtggac aacgaccgct
     1501 accggatagg cggcgagtat cgccaggtaa tgctttcgcc ccgggagctt tcgtatcagg
     1561 cgctgccttc gaggtcgtgg atcaacgaac acatcattta cacccatggc tacggagccg
     1621 tcatggggcc ggtaaaccgc atcaccagag aggggttgcc ggaattcttc atcaaagaca
     1681 tcccgccggt gtccctgacg gacatcaagg tgacccgccc ggaaatctac tacggtgaga
     1741 tagggaacga atatgttttc gtaaaaacca aacggccgga attcgattac ccggtcgggg
     1801 agaagaatgt ctattcccgg tacgagggca ggggaggggt cccgctctcc ttcctgaaga
     1861 agctgatttt cgtcgcgcgc ttcggatctt tgacgattct gctttccgac gacatcaccg
     1921 acgagagccg agtgatgtac taccgccggg tgaaggacag ggtgtcgaag atcgccccct
     1981 tcgccacact cgacggtgac ccatacctgg tcatctcgcc ggaagggcgc ctgctgtggt
     2041 tcctcgacgg ctacacgacg accggccgat tcccctattc ggagcctacg ccggggctcg
     2101 gcaattacat ccgcaattcc ctcaaggcca tcgtcgacgc ttacgacgga acggtgcagc
     2161 tttacgtgag cgacgagacg gaccctataa tccggacgta caggaatatc ttcccgggtg
     2221 tgttcaagaa gctggaagag atgcccgccg aattgcgcgc ccacgtccgg tatccccccg
     2281 gcatgttatc cgtccaggcg cgtatgtaca aggcctatca catgcaagac ccgcaggtgt
     2341 tctacaacaa ggaagacctc tgggcggttc ccctgaagca cgggagcaac caggagatgg
     2401 aaccttatta cacgatcatg aagcttccgg gagaaaagaa agaagagttc atcctgcttt
     2461 tgcctttcac gccgagcagg aaggacaaca tgtcggcctg gatggcggcc cgctgcgata
     2521 tgccgaatta cggcaaggtc atcgtctata attttccgaa gcagaagctc gtctacgggc
     2581 cgaggcagat agaggcccgc atcgaccagg atacggaaat ctccaaacaa ctttcgctct
     2641 ggagccagag gggctcgcag gtcatccgcg ggagcctcct ggcaataccg atcgagaagt
     2701 ccattctcta tgttgaaccc ctttaccttg cggcggagaa agggcagctc ccggaattga
     2761 agcgcgtgat cgttgccttc ggcaacgcta tcgccatgga ggaaaacctg gaattgtcgc
     2821 tccagcgtat cttcggcggc gaaatcccgc gcgagaaggg tctcgagcgg gtcgccgcag
     2881 gaccggcgga gcgggagaag acggacagac aactagccct cgaggccctt tcccattacc
     2941 gcaaggcaca ggaatacctg agacagggca actggggcgc ttacggcgaa gagttgcgtc
     3001 ggatggacga tatcctcaag tcgatcgaga gcaggaggta gtacgttcag agaatccctt
     3061 tcctcttcag gagaggtaag ggattaacga gcaggcgctt gaggcccatg gccctattgg
     3121 taaacccgta ggcaaggccc atcagttggg taaaatagaa gacgggtaag tcgtacctca
     3181 cgccgcgggt tgattcgacc tcccattgcc gtgtgtcgag gttgacctga cacatgggac
     3241 aggcgacggc gagggcctcg gcgccggcct ggcgagccgc ttcgaggacc tggcctccga
     3301 ggtctacgac gatacttgtt tccgaggcgg cgaaggcccc gccgcagcat tcggccttgt
     3361 gcgaccaatc aaccgtttcg gcgccgaggg cctgaagaat gcggtccatg gaccggggtt
     3421 gttctgggtc gtcgaatcgc gccaccgccc tggggcgcgt gacgaggcac ccgtaatagg
     3481 ccgcaaggcg gaggcctccg agcggcttaa taacagaaga tgcgattttc tccgtaccgg
     3541 cggcttcggt caaaagttgg agaagatgca tcacttccgg cgcgccgctt tcttttttcg
     3601 cttccatggc cgcacctttc aaccggatgg aacagccggg gcagggcgtg gtcaccgtgg
     3661 cgaggttcat cctctcgacg atggcgagat tcctggcact caagccccgc gtcagctcgt
     3721 ggttgagact atgggcggcg gtggcgccgc agcagttcca gtccgggacc tcctggagac
     3781 cgaagccgag acggtcgcag acgaggcggg ccgaacggtc gtattcgcgg gccacgcctt
     3841 cgagggaaca tcccgggtaa taggcgacta tcatccccgt tttacgctcc ttcttttcca
     3901 cgaagcaaaa agaaccctta cctcatccat ctttttcacc ttaccggccg tcaggggaag
     3961 cttgcccttg aggaacatcc ggatacccag cgtcaggtct ttcaggtact gcctggtcag
     4021 gatgttgaac ctgagcatca gaggtgcctc cgaaacgcga cccctccttt cgacacaaga
     4081 gcacataagc tcgtagaaaa gcgccaccgc ggggcggaag g
//
LOCUS       DAOTMX010000148         4092 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_17642_length_4092_cov_3.484023, whole genome shotgun sequence.
ACCESSION   DAOTMX010000148 DAOTMX010000000
VERSION     DAOTMX010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4092
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17642_length_4092_cov_3.484023"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /gene="flgL"
                     /locus_tag="P5573_05265"
     CDS             complement(<1..702)
                     /gene="flgL"
                     /locus_tag="P5573_05265"
                     /inference="COORDINATES: protein motif:HMM:TIGR02550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HRT27502.1"
                     /translation="MVTRVTENMKYFLLTNNLSSVQDRCGELMLQLSTLKRINKPSDD
                     PAGMTTVMNYRDMLASMEQYGRNIDGSASWLNITESTLSSVNDILVKIQEMALSQASA
                     TATAATREQAAAALQPLIDQILSLANTKLGDSYIFSGTKTGTKPFEAEAAVYAGGPAN
                     AAKGNTYKGTASVSGTYTGGTNITYVLKIVTGGDLDKATYQVSTDGGKTWGVESKVGD
                     LGAGTVDMGKGLSITF"
     gene            complement(720..2096)
                     /gene="flgK"
                     /locus_tag="P5573_05270"
     CDS             complement(720..2096)
                     /gene="flgK"
                     /locus_tag="P5573_05270"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HRT27503.1"
                     /translation="MSNLGSVMNVAKGALLSNASAINITGSNIANVNTPGYSRLRPIF
                     SSVGGAAELQFGVEVSGAERLYDKYLEVQLVQQNQKIGYYEARKGVLDRVEGIFNESE
                     GGGLNESLTKFWNAWEDLSANPTGTVQRDALVSAAESLATVFRQKAEELQEVQQDMNV
                     SVSETVSELNSYLASIAELNEKIVQTVTGGGNASDLLDKRQDNLQKLGAMIDFQYIED
                     ASGAVNVFTSNGKPLVEGGRTWKLETAVNPDNHDYYNVVFQDEPDEAINGCFSKGKLA
                     ACLEARDITVGGYLDKLDSLAANLVAAVNDQHRSGYDMYGNGGGNFFDPVTDAAQMRV
                     STAITEDQGKIAASTSVDRDGNNASAIAAIKDSLLMGGGTATLSDYYSALMARVGQDV
                     KDTNSSLDHQTVIMNQLKSQRETVSGVSLDEEMINLMKYQLGYNAAGRLAATVSALMD
                     TLMSMTES"
     gene            complement(2101..2601)
                     /locus_tag="P5573_05275"
     CDS             complement(2101..2601)
                     /locus_tag="P5573_05275"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HRT27504.1"
                     /translation="MDALYDSLIDILRREIEVYREIIEIIQEERRILLKPSLGGLDES
                     NARKETWVLKARLLEEVRMNLVERIAAGLGVPVEEANLSVLASAGGEDKGEMLRECQS
                     VLKALFQGLRDLNERNRALIDTSLQFLRDTAGFLNELLSPRTGYSGTGELKAVNRNGK
                     ILCVEG"
     gene            complement(2765..3070)
                     /gene="flgM"
                     /locus_tag="P5573_05280"
     CDS             complement(2765..3070)
                     /gene="flgM"
                     /locus_tag="P5573_05280"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HRT27505.1"
                     /translation="MKISKVDDAATQAIQQYQRNGKVQDASDRQVLGNAEPREKVDLS
                     AAAKDIGQLKGVIAELPDVREDKVNDLKSRIEQGTYNVSGEKIVDKMLGESIIDIFG"
     gene            complement(3141..3464)
                     /locus_tag="P5573_05285"
     CDS             complement(3141..3464)
                     /locus_tag="P5573_05285"
                     /inference="COORDINATES: protein motif:HMM:NF021638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HRT27506.1"
                     /translation="MDGTGRINEVVVGPQGATDAASRVKKEEEEKKLKKACTDFEAFF
                     VYYVLKTMRQTVPKGGLLGNSPGMDTYYMMMDQKIAENVAKKGDGLGLQKLLFDQLSK
                     KARTK"
     gene            complement(3473..>4092)
                     /locus_tag="P5573_05290"
     CDS             complement(3473..>4092)
                     /locus_tag="P5573_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HRT27507.1"
                     /translation="RSLTVNLYQPDFTTSMRLASLINSQVGDVEAKHVDSSSVLVRLK
                     DSYHGSVVGILSVIENLDVPVDSVAVVVMNEKTGTVVMGENVRISTVAVAHGNLSVEI
                     REEKKVSQPLPFAPASPEGGTPSAGTGANRNVIMGPGGQTVVTPESQVTAMEEKKRMM
                     LIPQGVTIQDVVRGLNAIGASPRDLITILQTIKAAGALQADLKII"
BASE COUNT          781 a         1260 c         1035 g         1016 t
ORIGIN      
        1 gaatgtaata gagaggccct tgcccatgtc gaccgttcct gcccccaggt cgcccacttt
       61 gctttccacg ccccaggtct ttccgccgtc agtagagacc tggtaagtag ccttatccaa
      121 gtccccaccg gtaacgatct tcagtacata cgtaatattt gtgcctcctg tgtacgtccc
      181 gctcacagat gctgtgcctt tgtatgtatt gcccttcgcc gcattcgccg gccccccggc
      241 gtacaccgcg gcctcggcct cgaagggttt cgtgcccgtc ttggtgcctg aaaatatata
      301 gctgtcgccg agcttggtgt tggctaaaga taggatctgg tcgatcaggg gctgcagggc
      361 ggcggccgct tgctccctag tggcggcggt ggccgtggcg ctcgcctgag acagggccat
      421 ctcctgaatc ttgacgagga tgtcgttgac gctcgacagt gtggattccg tgatgttgag
      481 ccaagaggcg ctgccgtcga tattgcgccc gtactgctcc atggaagcca gcatatcccg
      541 gtagttcatc acggtggtca tccccgcggg gtcgtccgag ggcttgttga tccgctttag
      601 cgtagaaagc tgcagcatca gttcaccgca tctatcctgg acgctggaaa ggttgttcgt
      661 gagcaagaaa tacttcatgt tttctgtcac acgggtaacc atgtctcaaa ctccccttat
      721 taactctccg tcatactcat caaggtatcc atcagcgccg acaccgtcgc agcgagtctc
      781 cccgccgcat tgtaaccaag ctggtatttc atcagattta tcatctcctc gtcgagagaa
      841 accccggaca ccgtttctcg ctgactcttc aactggttca ttatgaccgt ctggtggtcc
      901 aggctcgagt tcgtgtcctt gacgtcctgg cccacccggg ccatcaacgc cgaataataa
      961 tcgctcaggg ttgcggtacc gccccccatc aggagactgt ccttgatcgc ggctatcgcg
     1021 gaggcgttgt tgccatcccg gtcgacggat gtggaggccg ctatcttacc ttggtcttcc
     1081 gtgatggcgg tgctgacccg catttgcgcg gcgtccgtca ccgggtcgaa gaaattaccg
     1141 cccccgtttc cgtacatatc gtagcccgac cggtgttggt cgttcaccgc cgcgaccagg
     1201 ttcgccgcga ggctgtcgag tttgtcaaga tagccaccca ccgtaatgtc ccgggcctcc
     1261 aggcaggcag caagttttcc tttcgagaaa cagccgttga tggcttcgtc gggttcgtct
     1321 tgaaagacga cgttgtaata gtcgtgatta tccggattga cggccgtttc gagtttccag
     1381 gttcgtcccc cttccaccag gggttttccg ttggaggtga agacattcac ggcccccgag
     1441 gcgtcttcga tgtactggaa gtcgatcatc gcgccgagtt tctgcaggtt atcctgtcgt
     1501 ttatccagaa ggtcgctggc attccccccg ccggtgacgg tctggacgat cttttcgttc
     1561 aattccgcga tactggcaag atagctgttc aactccgata cggtctcgga cacggaaaca
     1621 ttcatgtcct gctgaacctc ctggagttcc tccgccttct gacgaaagac cgtagcaagg
     1681 ctttctgccg ccgaaaccaa ggcatcacgc tgaaccgtcc cggtggggtt cgccgagagg
     1741 tcctcccagg cgttccagaa cttggtcaga ctctcgttca ggccaccgcc ctccgattca
     1801 ttgaagatac cctcgacccg gtcgaggacg cccttacgcg cctcgtagta tccgattttc
     1861 tgattctgct ggacgagctg aacctccagg tatttgtcgt agagtctttc ggctccggag
     1921 acttcaacgc cgaattgaag ttccgccgcc ccgcccacgg aagaaaaaat ggggcggagc
     1981 ctgctgtatc cgggcgtgtt cacgttggca atgttggaac ccgtgatgtt gatagccgat
     2041 gcattactga gaagggcgcc ttttgccaca ttcatgacgc tgccgaggtt cgacatcttc
     2101 ctatccctcc acgcagagta tctttccgtt tctgttcact gccttcaact cccccgtacc
     2161 cgagtaaccc gtcctcgggg acaaaagctc gttcaaaaat ccggcggtat cccggagaaa
     2221 ctgaagggat gtgtcgataa gggccctgtt cctttcattc aggtcgcgaa gtccctggaa
     2281 gagcgccttc agcacggact gacattcccg caacatttca cccttatctt ctcccccggc
     2341 ggaggccaag accgaaaggt tcgcctcttc aacaggcacg ccgagtcccg cggctattct
     2401 ctcgaccagg ttcatccgca cctcctcgag gagcctcgcc ttcaagaccc acgtctcttt
     2461 cctggcgttg ctttcatcga gcccacccaa cgagggcttc aggaggatcc gcctttcttc
     2521 ctgaattatc tcaataatct cgcggtatac ttcgatttcc cttctcagga tatcgatcag
     2581 ggaatcatag agtgcgtcca tgttttctcc gacggcttga gtcatcttgc cctgcctgta
     2641 gctgcaacat gcattccgcc cggaggaaaa acacagagct atcggaacac ctcaccggtg
     2701 acccacctca aggcggacct caagaggtgt gatgtgggaa gaagggagag gaaaaaggtg
     2761 ccggtcagcc gaaaatatcg atgatcgact cgccaagcat cttatctact atcttctctc
     2821 cgctcacgtt gtacgtcccc tgttcgatgc gggacttcag gtcattgacc ttgtcttccc
     2881 ggacatcggg gagctccgcg atcacaccct tcaactgtcc gatgtccttc gccgcagcag
     2941 agagatcgac cttttcccga ggctcggcat tccccagaac ctgcctgtca gaggcgtctt
     3001 gcacttttcc gttcctctga tactgctgga ttgcctgggt cgccgcgtcg tcgactttgg
     3061 aaatcttcat ttctgctcct cgtgctttta ctatcatcta tcggcaaaag gacataaaac
     3121 tttagccgaa actactcttt ctattttgtt ctcgcctttt tactcaactg gtcaaaaagc
     3181 agcttctgca accccagtcc gtcgcccttc ttcgcaacat tttcggcgat cttctggtcc
     3241 atcatcatgt agtaggtatc catcccgggg gaattcccca ggaggccgcc cttaggaacg
     3301 gtctgcctca tagtcttgag cacgtaatag acgaaaaagg cttcgaaatc ggtgcaggcc
     3361 ttctttagtt tcttttcctc ctcctctttt ttcaccctgg atgcggcgtc ggtcgcacct
     3421 tgggggccca ctaccacctc gtttatccgt ccggtcccgt ccatatccac ccctatatta
     3481 tcttcaggtc cgcctggaga gccccggccg ccttgattgt ctgcaggatc gtgatcaggt
     3541 ctctgggaga agcgccgatg gcatttaggc ccctgaccac atcctggatc gtcacgccct
     3601 gaggaataag catcatccgc tttttctctt ccatcgccgt gacctgagat tccggcgtca
     3661 ccactgtctg acccccaggg cccatgatga cattgcggtt cgctcccgtc cccgccgacg
     3721 gcgtacctcc ctcaggggag gctggggcga aggggagggg ttgggagacc tttttctctt
     3781 ccctgatctc aacgctcagg ttcccatggg cgacggcaac ggtcgaaatc ctcacgttct
     3841 cacccatgac cactgttccg gtcttctcgt tcatcaccac aacggccacc gaatccacgg
     3901 ggacgtcgag gttttcgatg accgaaagga taccgacgac gctgccgtga tagctatcct
     3961 tcaatcgcac caggacagag ctcgagtcga catgcttcgc ttccacgtcg cccacctgag
     4021 aattgatcaa cgaggcaagc ctcatcgacg ttgtaaagtc cggctggtat agattcacgg
     4081 tcagggacct gt
//
LOCUS       DAOTMX010000149         4052 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_17851_length_4052_cov_3.105579, whole genome shotgun sequence.
ACCESSION   DAOTMX010000149 DAOTMX010000000
VERSION     DAOTMX010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17851_length_4052_cov_3.105579"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /gene="coaBC"
                     /locus_tag="P5573_05295"
     CDS             <1..942
                     /gene="coaBC"
                     /locus_tag="P5573_05295"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HRT27508.1"
                     /translation="PATANLIGKVAAGLADDLLSTTVMASRKPVLFCPAMNVNMYQNE
                     VVRHNMAMLSGRGYHILEASEGEMACGAEGPGRLPPLVDICEAVESILTEKDLEGENI
                     LVTAGPTQEPLDPVRFISNHSSGKMGYALAVMAKRRGAKVTLISGPTNLPSPSGVEFV
                     SVGSAVEMRDAVIDCMKEATVIIKAAAVADYRPARLSASKIKKKEGTFTLELERNPDI
                     IAEVGHVKGNRILIGFAMETENLVENARAKLISKNMDLIVANDLSSPEAGFQADTNIV
                     KLIDREGRIESLPLLDKKEVADRILSRIKPLRARTEK"
     gene            939..1670
                     /locus_tag="P5573_05300"
     CDS             939..1670
                     /locus_tag="P5573_05300"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRT27509.1"
                     /translation="MRERETQEDLRTELLEVVGSVKGYLLEAVDTGGKVRMFSTARMT
                     KADAPGEVESSGEVKSRVEQLAALEEELRSCRRCPLWTTRRTVVFGEGNPDARLMFVG
                     EGPGEEEDRQGRPFVGRAGQLLTRIINAMGLSRDDVYIANIVKCRPPGNRVPDQEEKD
                     ACTPYLCRQVEIIKPRVICALGSVAARFFLKTEAAVSVLRGRFHSLGSIDLMATYHPA
                     YLLRNPNAKKQVWEDVQEIMKRLKQ"
     gene            1690..2991
                     /locus_tag="P5573_05305"
     CDS             1690..2991
                     /locus_tag="P5573_05305"
                     /inference="COORDINATES: protein motif:HMM:NF012783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HRT27510.1"
                     /translation="MRARRILGAALFLLLSLTLGHSASWADGKPPVFDVISVNSAITP
                     PVSEYIVKSIEGSTRDGSAGVIIELDTPGGLDSAMRDIVKCLLNSKIPVFVYVSPPGA
                     RAASAGAIITVAANIAAMAPGTNIGAAHPVGIGIGKMDETMVEKVVNDMAAYARGIAV
                     QRGRNAEWVEKAVRKSVSVTAEEALRLRVIDYVAPDVESLLNQTDGKEVILASGKTTL
                     KTKGAVVNRKKMGFRERVLTTISDPNIAYLLMLIGLAGLYFEFAHPGAVIPGVVGGIS
                     LILAFFALQTLPVNYAGVLLILLAIILFIAEVKVVSHGILTIGGIVSLILGSLILYES
                     PDPALRVSLGVMVPAIVLVSLFFVAVISLAVKAQLRKPETGMEGMVGKEGVAITAIHQ
                     AGKVLVGGEYWNAESERGISEGSRIKVLGMKGLKLIVEEIK"
     gene            3010..3768
                     /locus_tag="P5573_05310"
     CDS             3010..3768
                     /locus_tag="P5573_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HRT27511.1"
                     /translation="MYMYTLLVIIILVVMFLSAAIKILNEYERAVIFRLGRVIATKGP
                     GLIILIPVVDRMVRVDMRTITMDVPPQDVITRDNVSIKVNAVVYFRVVDANRAIIEVE
                     NYLYATSQLSQTTLRSVCGQVELDEILAEREKLNVHLQEILDRSTDPWGIKVTHVEVK
                     YIDLPQEMQRAMAKQAEAERERRAKVINAEGEFQAAQKLADAARVISDQPIALQLRYL
                     QTLVQIGTENNSTTIFPVPIDLFKPFMRLADKKE"
     gene            3833..>4052
                     /locus_tag="P5573_05315"
     CDS             3833..>4052
                     /locus_tag="P5573_05315"
                     /inference="COORDINATES: protein motif:HMM:NF039187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HRT27512.1"
                     /translation="MEPREKRQILTGGLILIALGVLILLNSLNIYSFAKSWPILLVVT
                     SAATLFQNVRDAVGWIIGAVGVAFLLFEN"
BASE COUNT          975 a         1042 c         1233 g          802 t
ORIGIN      
        1 cccgcgacag cgaacctcat cgggaaggtc gcagccggac tggccgatga cctcctgtcg
       61 acgacggtga tggcatcgag aaagccggtg ctcttctgcc ccgccatgaa cgtcaatatg
      121 taccaaaacg aggtagtcag gcacaacatg gcgatgcttt ccgggagagg ctatcacatc
      181 ctcgaagcga gcgaaggaga gatggcctgc ggagcggagg gacccgggcg gctcccaccg
      241 ctcgtagata tctgcgaggc agtagagtcg atcctgacgg agaaggacct tgagggagag
      301 aacatcctgg tgaccgcagg gccgacacag gaaccgctcg accccgtcag gtttatttcc
      361 aatcattcgt cgggcaagat ggggtatgca ttggccgtca tggcgaaaag aaggggggcg
      421 aaggtgacgc tcataagcgg ccctaccaac cttccatcgc ccagcggcgt ggagtttgtt
      481 tccgtcggaa gcgccgtcga gatgcgggac gccgtaatag actgtatgaa agaggcgacc
      541 gtgatcataa aggcggccgc cgttgccgat tacagaccgg caaggctatc cgcctcgaag
      601 ataaagaaaa aggaagggac ttttactctc gaactggaga ggaatcccga catcatcgcc
      661 gaggtcggac atgtgaaggg caaccgcatt ctcatcggtt tcgccatgga gaccgagaat
      721 ctcgtcgaaa acgcccgagc caaactgatc tccaagaaca tggatttgat agtcgccaac
      781 gacctgagtt caccggaagc cgggttccag gcggatacga atatcgtcaa gctcatcgat
      841 cgtgagggga ggatcgagtc cctccccctc ttggacaaga aagaggtggc agacaggatc
      901 ctcagccgta tcaagccctt aagggcgagg acggaaaaat gagggaaaga gagacccagg
      961 aggaccttcg caccgaactg ctagaggtcg tcggttccgt gaaaggttat cttctggaag
     1021 ccgtcgacac aggcgggaag gtccgaatgt tttctacggc gaggatgaca aaggccgacg
     1081 cgccaggaga agtcgagtcg tcaggtgagg ttaagtcacg ggtggagcag ttggcggccc
     1141 tcgaagaaga gctccggtcg tgtcgcagat gtcccctatg gaccacaagg aggaccgtgg
     1201 tgttcggaga agggaatccc gatgcacgcc tgatgttcgt cggcgagggt ccgggcgaag
     1261 aggaagaccg tcaggggagg cctttcgtcg gccgggcggg ccagttgctg acacggataa
     1321 tcaatgccat gggtctttca cgggacgacg tttatatcgc caacatagtc aagtgccgtc
     1381 cgcccgggaa ccgcgtacca gaccaggagg agaaagatgc ctgcacccct tacctctgtc
     1441 ggcaggtcga gatcataaaa ccgagggtga tctgtgccct cgggtcggtg gcggcgcgtt
     1501 tttttctcaa gacggaagcg gctgtgtcgg tcctgcgggg acgatttcat agtttaggct
     1561 ctatcgacct catggcaacc tatcacccgg cttacctcct gagaaaccca aacgcgaaga
     1621 aacaggtctg ggaagacgtt caggagatca tgaagagact gaaacagtga ttgcgaggaa
     1681 aggttttgta tgagagcgcg tcggattctc ggtgcagcgt tatttttgct gctttccctt
     1741 actttgggac attcagcttc gtgggcggac ggcaagccgc cggtcttcga cgtgatttcg
     1801 gtgaactcgg caatcacgcc gccggtgtcg gagtacatcg tcaagagcat cgaggggtcg
     1861 acgcgagacg ggtccgccgg ggtgatcatc gagttggata cgcccggcgg actggacagc
     1921 gccatgcggg atatcgtcaa gtgcctcctc aattcgaaga tccccgtctt tgtgtatgtc
     1981 tccccgccgg gcgcaagggc cgcatccgcc ggtgcgatca tcacggtggc ggccaacatc
     2041 gccgccatgg ccccgggaac gaatatcggc gccgcccatc ccgtgggtat cggtatcggc
     2101 aagatggatg aaacgatggt cgaaaaagtt gtcaatgata tggcggcgta tgcccgggga
     2161 atcgcggtgc aaaggggcag aaatgccgaa tgggttgaaa aggccgtaag aaaaagcgtg
     2221 tccgtgaccg ccgaagaagc tctgcggctc cgtgtcatcg attacgtcgc cccagacgtg
     2281 gaaagcctct tgaatcagac tgacggcaag gaggttattc tggcctccgg caaaacgacc
     2341 ctaaagacga aaggtgccgt cgtcaaccgc aagaaaatgg ggtttcgtga aagagtcctg
     2401 accacgatca gcgacccgaa tatcgcctat ctgctgatgt tgataggact cgccggtctc
     2461 tacttcgagt ttgcccatcc gggtgcggta atcccggggg tagtcggggg aatttccctc
     2521 atcctcgcgt ttttcgccct acagacactg ccggtcaatt atgcgggcgt gctcttgatc
     2581 ctgctggcga taatcctctt tatcgccgaa gtcaaggtcg tgagtcacgg gatactcacc
     2641 atcggcggga tcgtttcact gattctggga tcactaatac tctatgaatc gcccgacccc
     2701 gcgctcaggg tatcgcttgg ggtcatggtt ccggcgattg tgctcgtatc cctctttttc
     2761 gtcgccgtca tctcgctcgc cgtgaaggcc cagttacgaa aaccggaaac cggcatggaa
     2821 ggcatggtcg gcaaggaggg cgtcgccatc acagccatac atcaggcagg gaaggtattg
     2881 gtcgggggtg agtactggaa tgcagaaagc gaaagaggga taagcgaggg ttcgaggata
     2941 aaagttctcg gcatgaaggg cctcaaactg atagttgaag aaatcaaata aatcgggagg
     3001 tagttcaata tgtacatgta cactcttctg gtaataatca ttctggtcgt gatgtttttg
     3061 tcggcagcca tcaaaattct caatgaatac gagagggccg tcatcttcag gttgggtcgc
     3121 gtaatcgcaa cgaaaggtcc gggattgata atcctcatcc ccgtcgtcga ccggatggtc
     3181 agggtcgaca tgagaaccat cacgatggac gtccctccgc aggacgtaat cacccgggat
     3241 aacgtctcta tcaaggtgaa cgccgtggtc tatttccggg tggtcgatgc caacagggcc
     3301 atcatcgaag tagagaatta tctttatgcg acgtcgcagc tctcccagac gacgctgcgg
     3361 agtgtgtgcg gtcaggtcga gctcgacgag atcctggcgg agagggaaaa gctcaacgtt
     3421 catctccagg aaatcctgga caggagcacc gacccctggg gcatcaaggt aactcacgtg
     3481 gaggtgaagt acatagacct gccgcaggag atgcaaaggg cgatggccaa acaggcagag
     3541 gcggaaaggg aacgacgggc caaggtcatc aacgcagaag gtgagttcca ggcagcgcaa
     3601 aaactggccg atgccgcccg tgtcatttcc gaccaaccga tcgcgttaca gctccggtac
     3661 ttacagactc tggtacagat cggaaccgaa aataattcga cgaccatatt ccccgtaccg
     3721 atagacctct tcaagccctt catgaggctt gcggataaga aagaataggg ggccgcgtat
     3781 ctgcggcaca aggtaaaaag aaaactcacg caaacgaaag aaaggaaaac aggtggaacc
     3841 gagagaaaaa aggcagattc tgacgggtgg gctcatcctt atagcgcttg gagtattgat
     3901 actgctcaac agtctcaaca tctacagctt cgcgaagagc tggcctattc ttttggtcgt
     3961 cacctcggcg gcgacgctgt tccagaacgt acgcgacgcc gtcggttgga tcataggggc
     4021 ggtgggtgtc gctttcctgc tgttcgagaa tt
//
LOCUS       DAOTMX010000150         3925 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_18524_length_3925_cov_4.073902, whole genome shotgun sequence.
ACCESSION   DAOTMX010000150 DAOTMX010000000
VERSION     DAOTMX010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18524_length_3925_cov_4.073902"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="P5573_05320"
     CDS             <1..357
                     /locus_tag="P5573_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27513.1"
                     /translation="EEFEPVAQLSVPSYTSWADMERDLTAWLGNHMQNDAVTVLYGLE
                     KKVRRKKDPEALEMWRRLQTSDHLYYMCTKWFADGTVHKYFNPYHSPYDAYINFMNIL
                     TDFSNTLEKAGRKHGR"
     gene            347..>3925
                     /gene="glgP"
                     /locus_tag="P5573_05325"
     CDS             347..>3925
                     /gene="glgP"
                     /locus_tag="P5573_05325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HRT27514.1"
                     /translation="MADDVFLFETSWEVCNKVGGIYTVIRTKAPFAVQNFGENYYLLG
                     PDLKANPEFEETDEECWVKIREATAIKEIPCRFGRWKIPGEPKVILISFGKKFNKDQL
                     LYELWEDYGVDSISGGWDYVEPVMFSYTCGAVIETVYNVVVRPTGGSAVAQFHEWMCG
                     AGLLCIKKRVPDVGTIFTTHATILGRSLSGAGVDIYAKMEQISPQKEAAAQNIMAKYS
                     METATAREADCFTTVSQITDVEAKNFLGRSSDVILTNGLDMENVPNYSVNRKVPLKYR
                     ERLVEFASRFLRKKLDAGARLMLISGRYEFHNKGIDVFLQALGRLDKDIRGKDMAEVV
                     ALICVLGEHMAINPCALQPGPLDGGVPLISTHRLYNEQGDPILNACSNLGLKNLPENH
                     VNIIFVPAFLNGHDGLINMDYYEVLSACDLGVFPSYYEPWGYTPHESVAYSVPTITTD
                     QAGFGLWVQNAVGQNGGVLILNRRGVPFNVIVDNLRGMLAESISWTEEDYAERRQEAR
                     KVALQAGWSNFYDRYLEAYSRASTTARARTSSRATADLRAKEGHVFAGTASTQPHFRS
                     FTAVVSLPSEIGRLRELAYNLWWSWHPSVLELFAHLDPKKWNESDNNPVRMLENVSPE
                     RLMEVAASEGYRNLYGRIISEFDGYMKAKMTGNPELPAELRWSSPIAYFSPEFGLHES
                     IQVYSGGLGVLAGDLLKAASDMNIPLVGVGLLYRNGYFRQKIDKDGWQIAEYEEGDFS
                     SMPVQIVRDEKGHDVQIAVELPGRTLFANIWEVRVGRVNLYLLDSDVARNTVQDRRIT
                     SRLYNDEPRARVEQEILLGIGGVKLLKKLGMKPSAYHINEGHSAFLIFERINDLMIDE
                     GLSFEEAKEVVRSNTCFTTHTPVDAGNERFSKDIIEYYFSNFVKRWGINWSQFWELKR
                     RESADESPFSMTVLGLKMSAMSNAVSRMHCEVAKRMWRDVWKGFHHTDVPIKSITNGV
                     HLLSYVAPEMREVFDVYLGRGWEKRMSDGQLWKKVQDIPNSYLWRIRSTLKFNLVNFL
                     REVISRQWMKYRESKLWREELVARINPAALTIGFARRFAPYKRAYLLLSDPDRLDRIV
                     NNDKCPVQVVFAGKAHPSDKAGIDLIKKVIDFCNDRRFIGKLFFLEDYDLRMAKHLIR
                     GVDLWVNTPRRPFEACGTSGQKVVINGGLNLSVSDGWWCEGFNGSNG"
BASE COUNT          970 a          946 c         1156 g          853 t
ORIGIN      
        1 gaagaatttg aacccgttgc ccagttgagc gtccccagtt acacctcctg ggcggatatg
       61 gaacgggatt tgacggcgtg gttgggaaac catatgcaga atgacgcggt caccgttttg
      121 tacggcctgg aaaaaaaggt caggagaaag aaggatccgg aagcgctcga gatgtggaga
      181 aggcttcaga cgtcggacca cctctattac atgtgtacaa aatggttcgc cgacggaact
      241 gtgcacaaat acttcaatcc ttaccattcc ccttatgacg cgtacatcaa tttcatgaac
      301 atcctcactg atttttcgaa taccctggag aaggcgggac ggaaacatgg cagatgatgt
      361 ttttcttttc gagaccagct gggaagtttg caataaagtc ggcgggatct atacagtaat
      421 aagaacgaag gctccctttg ctgtccaaaa tttcggcgaa aattactacc tgctgggacc
      481 ggacctgaaa gcgaaccctg agttcgaaga aacggacgag gaatgttggg tcaaaattcg
      541 cgaggcaacg gccatcaaag aaataccctg ccgtttcggg cgatggaaga taccgggtga
      601 accgaaggtc atcctcatct ccttcggaaa gaaattcaat aaagaccagc ttctttacga
      661 gctctgggaa gactacggag tagactcgat ctcaggcggg tgggactacg tggaacccgt
      721 catgttcagc tatacctgcg gggcggtcat cgaaactgtc tacaatgtgg ttgtcaggcc
      781 gacagggggg agtgctgtag ctcaatttca cgagtggatg tgcggtgccg gccttttgtg
      841 cataaagaaa agggtacccg acgtcggcac gatcttcacg acccatgcca cgattctcgg
      901 gaggtccctg tctggtgctg gcgtggatat ttatgcgaag atggagcaga tttctcccca
      961 gaaagaggcc gcagcgcaga acatcatggc aaaatattcg atggaaacgg cgacggcccg
     1021 ggaggcggac tgcttcacga cggtaagcca gatcaccgat gtcgaggcca agaacttcct
     1081 cggccggagt tccgacgtca ttctgaccaa cgggctcgac atggagaacg tgccgaacta
     1141 ttcagtcaac aggaaggtgc ctttgaagta tcgggagagg ctggtcgaat tcgcctcgag
     1201 gtttcttcgg aaaaaattgg atgccggggc gcgattgatg ctgatatcgg gcagatatga
     1261 atttcacaac aaaggcatcg atgttttcct gcaggccctc ggtcgtctgg acaaggacat
     1321 cagaggaaag gacatggctg aggtcgttgc cctgatctgc gtgctgggag aacatatggc
     1381 catcaatcct tgcgcgctcc agcccgggcc gttggatggc ggagtccccc tcatctcgac
     1441 tcatcgcctg tacaatgagc agggcgaccc gatcttgaat gcatgcagca acctcggcct
     1501 caaaaatctt ccggaaaatc atgtcaatat catcttcgtc cccgcgtttc tcaacggtca
     1561 tgacggactc atcaacatgg attactacga ggtgctttct gcctgtgacc tcggtgtctt
     1621 tccgtcttac tacgaaccat ggggttatac cccacacgag agcgtcgcat acagcgttcc
     1681 gacgataact accgatcagg ccggcttcgg gctgtgggta cagaatgcag tcggacagaa
     1741 cggaggagtt ctcatcctga acaggagagg agtgccgttc aacgtgatcg tagacaacct
     1801 gcgtgggatg ttggcggaat cgatttcctg gacggaagag gactacgctg agcgtcgaca
     1861 agaggcgagg aaagtggcgc tgcaggcggg gtggagcaat ttttacgaca gataccttga
     1921 ggcttacagt cgagcctcga cgacagcaag ggcgcggacc tcaagtcgtg cgaccgcaga
     1981 cttgagggcg aaggaaggtc atgtctttgc cggcacagcc tcgacacaac cccatttccg
     2041 gagttttacg gcggtcgtca gcttgccgag cgagatcggc cgcctcaggg aacttgccta
     2101 caacctatgg tggagctggc atccaagcgt tcttgagctc tttgcccatc tggaccccaa
     2161 aaaatggaac gaatcagata acaatcccgt ccggatgctg gaaaatgttt cgccggagag
     2221 actcatggag gtggcggcca gtgaaggtta ccgaaacctg tatggacgga tcatctcgga
     2281 attcgacggg tacatgaagg caaaaatgac cggcaaccct gaattgccgg ctgaactcag
     2341 gtggtcgtcg ccgatagcct atttttctcc cgaattcggt ttgcatgaaa gcatccaggt
     2401 ctattcggga ggtctcggcg tgcttgcagg agaccttctc aaggcggcga gcgacatgaa
     2461 cattcccctg gtaggggtgg ggttgcttta cagaaacggt tatttcagac agaagatcga
     2521 caaggacgga tggcagatcg cagaatatga ggagggcgat ttttccagca tgcctgtcca
     2581 gatcgtgcgt gacgaaaagg ggcacgacgt acagattgcg gtcgaactcc cggggaggac
     2641 gcttttcgcg aatatctggg aggtccgggt cggacgggtt aatctttatc tgctggactc
     2701 ggatgttgcg cggaataccg ttcaggacag aaggattacc tcgagactgt acaatgacga
     2761 gccccgagcc agggtcgagc aggaaatact gctcggtatc ggtggggtaa aactgttgaa
     2821 gaagctgggt atgaagccaa gtgcctatca tatcaacgaa ggacattccg cgttcctgat
     2881 ttttgaacgt ataaacgacc tgatgatcga cgagggtctc tcgttcgagg aagcaaaaga
     2941 ggtcgtcagg agcaatacct gtttcaccac ccatacgccg gtagatgccg ggaacgagcg
     3001 tttcagcaag gatatcatcg agtattactt ctcaaacttc gtcaagagat gggggattaa
     3061 ctggtctcaa ttctgggaac tcaagcgcag ggaaagcgcc gatgagagcc ctttctccat
     3121 gacggtcttg ggtctcaaga tgtctgcgat gtcgaatgcc gtcagtagga tgcactgcga
     3181 ggtggcaaaa aggatgtggc gcgatgtatg gaaggggttt catcacaccg atgtcccgat
     3241 aaagtcgatc accaacggtg ttcacctgct gtcctatgta gctccggaga tgcgggaggt
     3301 ctttgacgtc tacctggggc gcggctggga gaagaggatg tcagacgggc aactgtggaa
     3361 aaaagttcag gatattccga attcctacct atggaggata aggagtaccc tcaagttcaa
     3421 tctcgtcaat ttcttgcggg aggtcatctc caggcaatgg atgaagtaca gagagtccaa
     3481 gctttggcgg gaggagctgg tggcgaggat caacccggcg gcgctgacga tcggctttgc
     3541 ccggaggttc gctccgtaca agagggccta tcttttgttg tccgacccgg accgtctcga
     3601 ccgcatcgta aacaacgaca aatgccccgt tcaggtcgtt ttcgcgggaa aggcccatcc
     3661 cagcgacaaa gcgggcatag acctcatcaa aaaggtcatc gatttttgca atgacaggag
     3721 gttcatcgga aagctattct ttttggaaga ctatgacctc cggatggcca agcatctcat
     3781 ccggggtgtg gacctatggg taaacacccc caggcgaccg tttgaggcat gcgggacaag
     3841 cggacagaag gtcgtcatca acgggggtct gaacttgagc gtttctgacg ggtggtggtg
     3901 tgaagggttc aacgggtcga acggg
//
LOCUS       DAOTMX010000151         3889 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_18743_length_3889_cov_3.632760, whole genome shotgun sequence.
ACCESSION   DAOTMX010000151 DAOTMX010000000
VERSION     DAOTMX010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3889
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18743_length_3889_cov_3.632760"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /gene="prfA"
                     /locus_tag="P5573_05330"
     CDS             complement(<1..844)
                     /gene="prfA"
                     /locus_tag="P5573_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HRT27515.1"
                     /translation="MFGKLKEIEKRYTELEALLGDPSVVGNSAEYQKYAKEHADLRDI
                     VEMYRKYERLRLQVEEDQQLLRDGDEELRELIKEEIPQLKKELEETEAKLRVLLLPKD
                     PNDSRNIFLEIRAGTGGEEASLFAADLFRMYARYAETCGWKVEVVSSSPSSGLGGFKE
                     VIAMIEGKGAFGRMKYESGVHRVQRVPVTEAQGRVHTSAVTVAVLPEAEDVEVHIDPN
                     DLKVDVYRSGGHGGQSVNTTDSAVRITHIPTGLVVICQDERSQHKNKAKAMKVLRARL
                     LDRMT"
     gene            complement(1043..1264)
                     /gene="rpmE"
                     /locus_tag="P5573_05335"
     CDS             complement(1043..1264)
                     /gene="rpmE"
                     /locus_tag="P5573_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014697411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HRT27516.1"
                     /translation="MKEGIHPEYTETTITCVCGNVIKTRSSKKDIKVEICSNCHPFIT
                     GKQKIIDTAGRVERFQKKYEGKEKGGNKK"
     gene            complement(1372..2619)
                     /gene="rho"
                     /locus_tag="P5573_05340"
     CDS             complement(1372..2619)
                     /gene="rho"
                     /locus_tag="P5573_05340"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HRT27517.1"
                     /translation="MNVVEMKRKPISELAVMAKDLNVPGASGMRKQDLIFAILQAQAE
                     KNGVISGEGVLETLPDGFGFLRAVDYNYLPGPDDIYVSPSQIRRFGLRTGDTVSGEVR
                     PPKEGEKYFALLKVDSVNFESVEAARDKILFDNLIPLYPEEKLKLEYDPENYSTRIMD
                     LFTPIGKGQRGLIVSPPRAGKTVLLKDIAHSITANHKEVILMVLLIDERPEEVTDMER
                     NVKGEVISSTFDEPATRHVQVAEMVIEKAKRLVEHKRDVVILLDSITRLARAYNAVVP
                     PSGKVLSGGVDSNALHKPKRFFGAARNIETGGSLTIIATALVDTGSRMDEVIFEEFKG
                     TGNMELHLDRRIADRRVFPAFDLVRSGTRKEELLIPKENLNRIWILRRLLQEMNPIDA
                     MEFIVDKIRKTETNQMFLDSMNQ"
     gene            complement(2904..3701)
                     /gene="pgeF"
                     /locus_tag="P5573_05345"
     CDS             complement(2904..3701)
                     /gene="pgeF"
                     /locus_tag="P5573_05345"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HRT27518.1"
                     /translation="MFRLLERGRVCYLESARLKENPFLTHAFLTRWSGVSTGKFSSLN
                     LGLREGDKEERVARNWRLIADAFGLSVSQFFPIDQVHGDRIVVVEDLQDETSPVRCDA
                     VLTARRGVALAVKTADCVPLLLVDREKQVVGVVHAGWRGTALGIAAKAVKTLKERFDS
                     KPVDILAAIGPAVGACCYQVDGKVYDAFKKSVSVRERAFRACQDVEGRWMLDLESANI
                     IQLVEAGIPRENIFSAALCTSCRRDLFFSHRAERGGTGRHLSFIVLN"
     gene            complement(3710..>3889)
                     /locus_tag="P5573_05350"
     CDS             complement(3710..>3889)
                     /locus_tag="P5573_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27519.1"
                     /translation="MLRSRLKGMRRQALHARGLGLYHPLTSRYLWFESPLPEDMQRVV
                     AYLREYTRGNHGVEG"
BASE COUNT          778 a         1158 c          993 g          960 t
ORIGIN      
        1 ccgtcatccg gtcgagcagc cgtgccctca agaccttcat cgccttcgct ttattcttgt
       61 gctgggaccg ctcatcctga cagatgacga cgaggcccgt cgggatgtgg gtgatcctga
      121 ctgccgagtc cgtagtgttc acgctttggc cgccatgacc cccggaacga tagacgtcca
      181 ctttgaggtc gttcgggtcg atgtgaactt cgacgtcctc cgcctcggga aggaccgcta
      241 cggtgaccgc tgaggtgtgg actcgcccct gcgcctcggt gacggggacc cgctgtacac
      301 ggtgcacgcc gctttcgtac ttcatccggc cgaaggcgcc ttttccctcg atcatggcga
      361 tgacctcctt gaacccgccc aagccgctgg agggactgct gctcacgacc tccactttcc
      421 atccgcaggt ttcggcgtag cgggcataca tccggaacag gtcggcggca aacaggctcg
      481 cctcctctcc cccggtacct gctcggatct ccaaaaagat gttcctcgag tcattgggat
      541 ccttcggaag gaggagaacc cggagcttcg cttccgtttc ttccaactct ttcttcagtt
      601 gtggtatctc ttcctttatc agttccctga gttcctcgtc gccgtcgcgg agaagttgct
      661 ggtcctcttc aacctgaagc ctcagcctct cgtactttct gtacatttcg acgatgtcgc
      721 ggaggtcggc gtgctccttc gcatacttct gatactcggc gctgttgccg actacagagg
      781 ggtcgcccaa caaagcttcc agctccgtat atcgcttttc tatctctttc aacttaccga
      841 acatgataag ctgacctgcc ttttcgatga ggggattatt tgttaccgcg aaccaaagaa
      901 tgtcctggta ggagcttcac cgcttgtcgg gaaaggacct tgcgccggac acagggcaca
      961 agaaacaatc ggtcgttgag cattcaaccc gtatgagccg gacacacgga ccgccgcgcc
     1021 ctcccggccc tcacggcgca acttacttct tgttgccgcc cttctctttt ccctcgtact
     1081 ttttctggaa cctttctact ctgccggcgg tgtcgattat cttctgcttc cccgtaataa
     1141 aaggatgaca gttcgagcag atttccacct tgatgtcttt cttcgacgac ctcgtcttga
     1201 tgacgttacc gcaaacacag gtgatggtcg tttctgtgta ctcgggatgt atgccttcct
     1261 tcatgactgc tgtcgaatcc ccctgtcgga aaaaatgatt gttctttctt gctttcagaa
     1321 acttggcatt ttattcattc cggccataaa agcaagaaaa tatttcgtgc gtcactggtt
     1381 catggaatcc aggaacatct ggttagtctc cgtcttacgg atcttgtcca cgatgaactc
     1441 catggcgtcg atcgggttca tctcctggag gagcctcctc agtatccaga tcctgttgag
     1501 gttttccttg ggaatcagca attcttcctt ccttgtcccg gaacgcacca ggtcgaaggc
     1561 agggaagacg cgcctgtcgg cgatcctacg gtcgaggtga agctccatgt tgcccgtacc
     1621 cttgaactcc tcgaaaatga cctcatccat gcggctgccc gtgtcgacca aggcggttgc
     1681 tatgatcgtc aggctccccc cggtctcgat attccgggcg gcaccgaaaa acctcttcgg
     1741 cttatggagg gcattggagt ccacgccgcc ggaaagcacc ttgccgctcg gcgggactac
     1801 tgcgttgtaa gccctggcca ggcgagtgat actgtccagc aggatgacga cgtccctctt
     1861 gtgctccacc agccgtttgg ccttctcgat gaccatttcc gccacctgga cgtgtctggt
     1921 cgccggttca tcgaaggtag aggagatcac ttcgcctttg acgttcctct ccatgtcggt
     1981 cacttcttcc gggcgttcgt cgatgagaag aaccatcagg atgacttctt tgtgatttgc
     2041 cgttatgctg tgggcgatgt ccttgaggag gactgtttta ccggcccggg ggggagaaac
     2101 gatcaagcct cgctgtccct ttccgatcgg tgtgaagagg tccattatcc tggttgaata
     2161 gttctcaggg tcgtactcca gcttcagttt ctcttctggg tacaggggta tgaggttgtc
     2221 gaacaggatc ttgtccctgg cggcttcaac gctttcgaaa tttaccgaat cgaccttcaa
     2281 gagggcgaag tacttctctc cttctttggg aggtcttacc tcaccggaga ccgtgtcacc
     2341 cgtccggagg ccgaacctcc tgatctggga cggcgagaca tagatgtcgt cgggtcccgg
     2401 aaggtaattg taatccaccg ccctcaaaaa accaaaacca tcgggcagcg tttccaaaac
     2461 accctccccc gagatcacgc cgtttttctc cgcctgtgcc tggaggatgg caaaaatcaa
     2521 atcctgtttc ctcatcccgc ttgcaccggg cacattcagg tccttcgcca taacggccag
     2581 ttcactgatg ggttttctct tcatttccac gacgttcatg ttcgcgacct tttaaactcc
     2641 ttgtgtgggt tttaatgtga catcgagccc ccgcagacct tacgagagcc cggtcttaat
     2701 gcaccatgtt actacacgat accttgttaa ccggctggtt catcgatgag ttaaaaaaga
     2761 aaaaacgcag gtttttagta ggcagttaat ttggtgtctg attattgttt cggaaggaat
     2821 caatagaacc ttcttaaatt caacgataaa atacacgacg cagaaagaca tgtcaagctg
     2881 attttgattt ttgattttgt gaatcagttc aggacgatga aactcaggtg ccttcccgtc
     2941 cctcctcttt ccgccctgtg ggaaaaaaag aggtcccggc ggcaggacgt gcagagggcc
     3001 gccgaaaata tgttctctcg cgggatgcct gcttccacca actgtattat attggccgac
     3061 tcgaggtcga gcatccatct gccttcgaca tcctgacagg cccggaaggc ccgctccctc
     3121 actgagaccg actttttgaa ggcatcataa accttcccat cgacctgata acaacaggcc
     3181 ccgacagcag gcccgatcgc cgccaggata tccaccggct tcgaatcgaa cctctcttta
     3241 agggttttaa cggccttggc tgctatcccc aacgccgtcc ctcgccagcc ggcgtggacg
     3301 acccccacta cctgcttttc ccggtccacg aggagaaggg gaacacagtc cgccgtcttg
     3361 acggccaggg ccactcctct tcttgccgtc agcacggcat cgcaacggac aggggaggtt
     3421 tcgtcctgaa ggtcttcgac gacgacgatg cggtcgccgt ggacctggtc tatcgggaag
     3481 aattgagaga ccgaaaggcc gaaggcgtcg gcgatgagcc gccagttccg ggcaacccgc
     3541 tcttctttgt ctccttcacg caggccgaga ttcaagctgg aaaacttccc ggtgctcacg
     3601 ccgctccatc gggtcaggaa ggcgtgggtc aggaatgggt tttccttcag tcgcgccgat
     3661 tccaggtaac agacccgacc gcgctccaaa agcctaaaca tgctgtccgt caaccctcca
     3721 ccccgtggtt cccgcgagta tactcccgca agtacgcgac gaccctctgc atgtcctccg
     3781 ggagggggga ttcaaaccag aggtaccggc tcgtaagggg atggtaaaga cccaaccccc
     3841 gggcatggag ggcctgacgc cgcatacctt taagccttga acgaagcat
//
LOCUS       DAOTMX010000152         3885 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_18770_length_3885_cov_3.689034, whole genome shotgun sequence.
ACCESSION   DAOTMX010000152 DAOTMX010000000
VERSION     DAOTMX010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3885
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18770_length_3885_cov_3.689034"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..1259)
                     /locus_tag="P5573_05355"
     CDS             complement(36..1259)
                     /locus_tag="P5573_05355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HRT27520.1"
                     /translation="MPGIMLKSCPKVYMLETHRSKTPADTLRFVERVKEATGMMSFRN
                     ATDVDRIGIQVFTCDRRRPDGSLTSHTGKGVSAIQAQVSITMEAIERYCSEFRQEYLE
                     KLVKGPYRELRSRFNVLDPEEMILSQFNDYRPDKDIYWAWGYDIARKEDVLVPACAVY
                     HPFHEDQTRLINTNTNGVAAGNTMEEAVIHGLAEVIERDAWSIAQYTRRFNDAIFIED
                     GPANEFIVGVLEKFERAGVEVVAKDLTTDVGMPVVAAFSQDLKYRTMAPVDGFGAHLD
                     PRVATVRALLEIATTRVLLLQKYGIEGVCDTPPLYMRDCEEEEDPRFFAYDQKGINEL
                     EVGYSTDIYEDIMKVVAGLRACGLERVIAVDLTRPDMGIPTVRMVVPGMEAFCFDKTR
                     IGRRAQTAFEDDPYR"
     gene            complement(1328..2419)
                     /gene="mnmA"
                     /locus_tag="P5573_05360"
     CDS             complement(1328..2419)
                     /gene="mnmA"
                     /locus_tag="P5573_05360"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HRT27521.1"
                     /translation="MTEEKRHTADRKVLVAMSGGVDSTVAAFLLKEQGYDIAGVTMNL
                     RLKGVGDESARCCGPDVLEDARRVCERLGVAHHVVDYARELEERVVSRFVREYRRGRT
                     PNPCVDCNRHLKFGSLLGKAVAMGFDFLATGHYARIDRAGGGCLLRRPRDRRKDQTYF
                     LYSIPRDALRRIIFPLAPFTKEEVRRIAARAGLPLAEKKESQDICFVTQKNYGAFISQ
                     RIDGIRPGPITDLAGKKVGEHRGIVFYTVGQRSGLRVSAGRPLYVVSIDPAGNRIVVG
                     EKEDVLARGLIAGDINALVEEWPAVAYGKLRYRKKEEPCRVSVEGRKLRVMFEEKQEA
                     ITPGQSVVLYDGDVVLGGGVIEEVIGGVR"
     gene            2514..3536
                     /gene="bioB"
                     /locus_tag="P5573_05365"
     CDS             2514..3536
                     /gene="bioB"
                     /locus_tag="P5573_05365"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HRT27522.1"
                     /translation="MQTARTKASVRGGRSVGRNHIERMKEKALQRRPVAFDEALGLFD
                     SGMKQPFHLMAAASEIREHFKGKEISLCGIVNAKSGRCPENCKFCAQSAHYSSGAPVY
                     PLVSTAEMVENARRAVENGVRFFGIVTSGKSISAKKEWDTIREAVKLIDGLGVKVCAS
                     LGTIDRKKAAELKDAGLFRYHHNLETARSFFSNICTTHDYEEDVDAVRAAKEAGLSVC
                     CGGLIGLGENIAHRVELAITLRELDVDSVPVNILNPIPGTPLADVPALSPLEILVTVA
                     AYRFLLPEKDIKLCGGKEKNLRQLLPLGIVAGCNSWMTGNYLTTLGRDVYQDLEMIRD
                     LGLKVG"
     gene            3548..3847
                     /locus_tag="P5573_05370"
     CDS             3548..3847
                     /locus_tag="P5573_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27523.1"
                     /translation="MYLETARRWVAELQDSGKLSDLDSDALEKLAQEYARRLEEFYLE
                     EVARQMEKCGKAVEFERMLLYDGQYMNKYLNQTIPAYPAFRLEVFSKARKVILGE"
BASE COUNT          838 a         1173 c         1040 g          834 t
ORIGIN      
        1 caatatccca gcgattccgg gaaggtgacc ttatgctacc ggtatgggtc atcttcaaat
       61 gccgtttggg cccttctgcc gatccgggtc ttgtcgaaac agaaggcctc cattccgggg
      121 acgaccatcc tgaccgtggg tattcccatg tccggccgtg tcaggtcgac ggcgatcaca
      181 cgttccaatc cgcaggcacg caaacccgcg accaccttca taatgtcctc atagatgtct
      241 gtgctgtagc ctacttcgag ttcattgatg cctttctgat cataagcgaa aaagcggggg
      301 tcctcttctt cctcacagtc gcgcatgtag agcggcggcg tgtcgcacac cccctcgatc
      361 ccgtattttt gcagtaaaag gacacgcgtg gtggcgatct caagaagggc gcgtaccgtg
      421 gcgacccggg ggtccaggtg agccccgaag ccgtcaacag gcgccatagt ccggtactta
      481 aggtcttggg aaaaggcagc gacgaccggc atccctacgt ccgtcgtcag atccttggct
      541 acgacttcga cacccgctcg ctcaaatttt tcgaggacgc cgacgatgaa ttcgttcgcc
      601 ggcccatcct cgatgaatat cgcgtcgttg aaacgccgcg tgtactgggc gatgctccag
      661 gcatccctct cgatgacctc ggcaaggccg tggatgaccg cttcctccat ggtattcccc
      721 gcggcgacgc cgttggtgtt ggtgttgatc aatcgcgtct gatcctcatg aaacgggtgg
      781 taaaccgcgc aggcaggcac caatacgtcc tcttttcggg ctatatcgta cccccaggcc
      841 cagtagatgt ccttgtccgg acggtagtcg ttgaactgtg aaaggatcat ttcctccggg
      901 tcaaggacat tgaacctcga cctcagctcc cgataaggac ccttgacgag cttctccagg
      961 tattcctgcc gaaactcgga acagtatcgc tcgatcgcct ccatcgtgat cgagacctgc
     1021 gcctgtatcg cagagacacc cttgcccgta tgactggtca gcgatccatc cggccttctc
     1081 ctgtcgcagg tgaagacctg gatgccgatc ctgtccacgt ctgtcgcgtt cctgaacgac
     1141 atcataccgg tagcctcttt gaccctttcc acgaaccgca gggtgtctgc gggcgtttta
     1201 gaacggtgcg tttcgagcat gtaaaccttg ggacaactct tgagcattat tcctggcatc
     1261 ctgcacctct cccgaatgag attcaccgtg aatctaataa atcgaacgca aaaagaaaag
     1321 tgttttgtca gcggacccca ccgataacct cctcgatcac gccgccgccc aggacgacgt
     1381 ccccgtcata gagtacgacg gactgtccgg gggttatggc ctcctgcttt tcttcaaaca
     1441 ttacccgtaa cttccttccc tcgaccgaca cccgacaggg ctcctctttc tttctgtacc
     1501 tgagcttacc gtaagcaacg gccggccatt cctccaccaa ggcgttgatg tccccggcaa
     1561 tgagacccct cgccagaaca tcctcctttt cgcccacgac gatacggttc ccggccgggt
     1621 cgatggagac cacgtaaagg gggcgccccg cgctgacccg caacccactc cgctggccta
     1681 ccgtataaaa cacgataccc cggtgttcgc cgaccttctt gcccgcgagg tccgtgatcg
     1741 gccccggcct tatcccgtct atgcgttgcg agatgaaggc gccgtaattc ttctgggtaa
     1801 cgaagcagat atcctggctt tccttctttt ccgccaaggg cagtcccgca cgcgcagcga
     1861 ttcgcctcac ctcttccttc gtgaagggcg ccagtgggaa gatgatcctt ctcagggcgt
     1921 cgcgcggtat cgagtagagg aagtacgtct gatccttcct cctgtcccgg ggtcttctca
     1981 gaagacagcc tcctccggcc ctgtcgatcc ttgcgtaatg cccggttgcc aggaagtcga
     2041 aacccattgc aaccgctttc cccagcaggc tcccgaactt caagtgtcgg ttgcagtcga
     2101 cacagggatt gggcgttctg cctctcctgt attctctgac aaagcggctg actacccgct
     2161 cttcaagctc ccgagcgtaa tctacgacat gatgagctac gccgagtctc tcacagaccc
     2221 gacgggcgtc ttccaggaca tcgggaccac aacaacgggc actctcatcc ccgacacctt
     2281 tcagtcttaa gttcatggtc acgccggcga tgtcgtagcc ctgttcctta agcaggaaag
     2341 cggcgacggt agagtctacg cccccgctca tggcgacgag caccttcctg tcggcagtat
     2401 gtcttttctc ctcggtcatg tctccgaccc tccgccctcg tgaatgatac catactcgtc
     2461 aaataaaccg gttgacaaaa aaagaatatg cccttaagta tccacggaac agcgtgcaga
     2521 cagcgaggac gaaagcgagc gtccggggag gaaggtccgt gggccgaaat catatcgaaa
     2581 gaatgaagga aaaggcgctt caaaggcggc ccgttgcctt cgacgaagcc ctcgggctgt
     2641 tcgactcagg gatgaaacag ccctttcact tgatggcggc cgcatcggaa atccgggaac
     2701 attttaaagg caaggagatc agtctctgtg gcatcgtcaa cgcaaaatcg gggcgatgcc
     2761 cggaaaattg taaattctgt gctcaatccg cccactacag ttccggtgca cctgtctatc
     2821 cgttggtatc caccgcggag atggtcgaga atgcgcggcg tgccgtggaa aacggcgtcc
     2881 gcttcttcgg gatcgtaacg agcggcaaga gcatctcggc aaagaaggaa tgggatacaa
     2941 tccgggaggc ggtgaagctg atcgatggcc ttggggtcaa ggtctgcgca tccctgggga
     3001 ctatcgaccg gaagaaggcc gccgagctga aggacgcagg ccttttccgc taccatcaca
     3061 acctcgagac cgcacggagt ttcttctcga acatctgcac gacccacgat tatgaagagg
     3121 atgtcgacgc cgtgcgtgcg gcgaaagaag ccggtctttc cgtctgctgc ggtggactga
     3181 tcggcctcgg tgagaacatc gctcacaggg tcgaactcgc cattacactg agagagttag
     3241 acgtagactc cgtcccggtc aacatcctca acccgattcc cgggactccc ctagccgatg
     3301 tacccgccct ctcccccctg gaaattctcg ttaccgtcgc cgcttaccgg tttcttttgc
     3361 cggaaaagga cattaaacta tgcggcggca aagagaagaa cctgcggcaa cttctgcccc
     3421 ttgggatcgt cgccggttgc aactcatgga tgacgggcaa ttacctgacg acgctgggaa
     3481 gggatgtcta tcaagacctg gaaatgatca gagacctcgg cctgaaggtc gggtagtgga
     3541 ggttcttatg tacttagaaa ccgccagacg ctgggtcgcg gaattgcagg actcaggaaa
     3601 attgagcgac ctcgattccg atgctttgga gaaactggct caggagtatg caagaaggct
     3661 tgaggagttc tacctcgaag aggtcgcccg acagatggaa aaatgcggaa aggccgtgga
     3721 gttcgagagg atgctcctgt acgacggcca gtacatgaac aaatacctga accagacgat
     3781 ccccgcctat cccgctttcc gcctcgaggt cttcagcaag gcccgaaagg tcatcctggg
     3841 agagtgagcg caaaggcaga cttccgagga atgcccgatc ttgac
//
LOCUS       DAOTMX010000153         3860 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_18925_length_3860_cov_3.417608, whole genome shotgun sequence.
ACCESSION   DAOTMX010000153 DAOTMX010000000
VERSION     DAOTMX010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3860
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18925_length_3860_cov_3.417608"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="P5573_05375"
     CDS             <1..508
                     /locus_tag="P5573_05375"
                     /inference="COORDINATES: protein motif:HMM:NF012647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HRT27524.1"
                     /translation="KRLICLVDLYRAVEMERDEMIDSNIIRFIFSFENDDRGFGYIQS
                     NLTETSMALAILNMLDYPVKELETEQFIQRCETPLFGFTDVPHRSLSYMEYIHAGVLA
                     SSIISYCPHYHDRCVDFILNCQNTNGGFSRAPHAGIATMESTFFAVHALKLLSDLRRR
                     VVPPKSTS"
     gene            complement(492..1394)
                     /locus_tag="P5573_05380"
     CDS             complement(492..1394)
                     /locus_tag="P5573_05380"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HRT27525.1"
                     /translation="MKTTKFYRLMAVPLLVAVMGCAEVAKVGTEIGYQQGLITKEMKE
                     GIVKSAEQAEAASRPMTAEEEYFLGRAVGAEILSRYPLYRNERLTRYVNEIGNVVALS
                     SDRPVTYSGYHFAILDTDEANAFACPSGTIFVTRGMLRRTQNEDELAAVLAHEAGHVN
                     HRDGVNSIQKSRWAQVIATLGTEAARTLSGKDLAKLTDLFAGSVSDVVKTIIVNGYSR
                     DQELTADQSALVFLHRAGYDPRALTSYLGRLAKEQTGGASAGFFSTHPGMDKRLAAAQ
                     TYLDRQNWAPVDRTARDRRFKMYF"
     gene            complement(1391..1861)
                     /locus_tag="P5573_05385"
     CDS             complement(1391..1861)
                     /locus_tag="P5573_05385"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HRT27526.1"
                     /translation="MKKTCLFIILIVLATLSVASAAQYKIITKDAPIRKDNRFFAPVV
                     TRIPYGATVEDQGRQGDWLRVNYKGKQGWIHIGAVQERTVQLLRAGKAHEATQEEVAL
                     AGKGFTPEVEKAYRERNPRLKYGEIDEIESLLIDDDSLQAFIRAGNLNEPGGGR"
     gene            complement(1996..>3860)
                     /locus_tag="P5573_05390"
     CDS             complement(1996..>3860)
                     /locus_tag="P5573_05390"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HRT27527.1"
                     /translation="LAAGARAVVFDVLFTESSSYGVEDDLLLAEALRKGRTFLPVFLS
                     REERPQPTDEKLIIDRLALSMEDRLAQTRTPYKSIILPVDVLARSAYGLGNVEIPPDA
                     DGIYRRMPLVFLYRDKWMPALGLSAFAYGGEKLPTVLEENRLSVAGISIPLLSDGNFL
                     LRYYGDGREFPRFSAFNVIQSHLAIQEGGEPPYPPELFRDKIVFLGFTAAGLYDLKPT
                     PTSSIYPGAAIHATLVENLLRKDFRTRISTIPLLALTVAAAAAAALTVMLVASFWKLG
                     LLIIVYVVGLVLLIVIAFSRNLWVDSVLPLTGLLLSFAAATTFSYATEGRQRRQIKQM
                     FSHYMSDLLLEDLLKNPDKLRLGGEKRVLTVFFSDLAGFTSLSEKLKPEEVVSLLNRY
                     LTEMTDIIMESGGLIDKYEGDAIMAFWGAPIPQEDHAARACYAALDNQHRLAELRKEF
                     AAAGLPPVYSRIGINTGEMIIGNMGSSKRFDFTVIGDNVNLASRLEGAGKEYGTWIII
                     SEATFSQAADRVEVRELDFLQVKGKEIPVRIYELLSRKGALEDKMKEVRDHFETGLAH
                     YRRREWESALEAFRRALAASPDDGPSRTYIRRCEEFLENPPPDDWNGVYRLITK"
BASE COUNT          763 a         1105 c         1102 g          890 t
ORIGIN      
        1 caagcgcctg atctgcctgg tggatctata cagagccgtg gagatggaaa gggacgagat
       61 gatcgatagt aatatcatcc gttttatttt ttctttcgag aacgatgata ggggtttcgg
      121 ttatatccaa tcaaacctta cggaaacttc catggccctt gcaatactca acatgctgga
      181 ttacccggtc aaagaattgg agactgaaca attcatccag agatgcgaaa cgccgctctt
      241 cggcttcacc gatgttcccc accgttctct atcgtacatg gaatatatcc atgccggagt
      301 cctggcatcg tccatcatct cttactgtcc ccactatcat gaccggtgtg tcgatttcat
      361 cctgaactgc cagaatacca acggtgggtt ttcaagagcg cctcatgccg gcatcgccac
      421 gatggagagt actttttttg ccgtgcacgc cctcaagctc ctgtccgacc tccgtcgacg
      481 cgttgtgccc cctaaaagta catcttgaat cttcgatccc tcgccgtgcg gtccaccggc
      541 gcccagttct gccgatccag gtaggtctgc gcggcggcca gccgcttatc catcccggga
      601 tgggtggaga agaagccggc tgacgccccg ccggtctgct ctttcgccag ccgccccagg
      661 tacgatgtga gcgcacgggg atcatagccg gcccggtgca ggaaaaccag ggctgactga
      721 tcggcggtaa gctcctgatc gcggctgtag ccgtttacta tgatcgtttt tacaacgtcg
      781 ctgaccgaac cggcgaaaag gtccgtcagc ttcgccaagt cttttccgct gagcgttctt
      841 gccgcctcgg tccccagcgt ggcgatgacc tgggcccagc gggacttctg gatcgagtta
      901 actccgtccc ggtggtttac gtggcccgct tcatgggcca gtacggcagc cagttcatct
      961 tcgttctggg ttctccgcag catgccgcgg gtcacgaaga tggtgccgct ggggcaggca
     1021 aaggcgttcg cctcgtctgt gtcgagaatc gcgaagtggt aaccgctgta agtgacgggc
     1081 ctgtcggaag acagggccac gacgtttcct atttcgttga catagcgtgt cagccgctcg
     1141 tttcggtaaa ggggatagcg gctcaggatc tccgccccca cggcccgtcc gaggaagtat
     1201 tcctcctccg ccgtcatggg gcgactggcc gcctccgcct gttcggcgct cttcacgatc
     1261 ccttctttca tttccttggt tatcagtccc tgctgatacc cgatctcggt ccccactttc
     1321 gccacttccg cgcagcccat gactgcgaca agcaatggga ctgccatgag acggtaaaat
     1381 tttgtcgttt tcatcgacca cctcccggct cgttcaggtt tccggctcgt atgaaggctt
     1441 gcagtgaatc gtcatcgatg agaagggatt cgatttcgtc gatctcgccg tattttaaac
     1501 ggggattcct ttcccggtag gctttctcta cctccggggt gaatcccttg ccggcaaggg
     1561 ccacttcctc ctgcgttgct tcatgggcct tccccgctct caggagctgg actgtccgtt
     1621 cctggacggc gccgatatgt atccatcctt gtttcccttt gtaatttacc cgcagccagt
     1681 caccctggcg tccctggtct tcgacggttg ccccgtaagg gatgcgtgtg actaccggtg
     1741 cgaagaagcg attatccttg cggatcgggg cgtcttttgt gatgattttg tactgcgccg
     1801 ccgaggcaac actaagggtt gccaagacaa tgagaatgat gaataggcac gttttcttca
     1861 taaaccctcc tgccgatgat tgttcttgta tcttatacat tcgacgcctt gtatcttcaa
     1921 gtactacggt agacggcatc aatgattcgg catcggttag gggcgcgctg atgctgcttt
     1981 ctcttcttgc cgacactatt tcgttatcaa cctgtatacc ccattccaat catcaggtgg
     2041 cggattttcc aggaattctt cgcacctgcg gatataagtc cgggaagggc catcatcggg
     2101 gctggcggcg agggccctgc ggaaagcctc cagggcagac tcccattccc gccgacgata
     2161 gtgcgcgagc cctgtctcga agtgatcgcg cacctctttc attttatctt ccagtgcgcc
     2221 ctttctcgag agaagttcgt agattcggac cgggatttcc ttacctttga cctgcaggaa
     2281 gtccagttcc ctcacctcga cccggtcggc agcctgactg aaggtcgctt cgctgatgat
     2341 gatccaggta ccgtattcct ttccggcgcc ttccagccgg gaagcgaggt tcacgttgtc
     2401 cccgatgacg gtgaaatcga accgcttgct cgaacccatg ttgccgatga tcatctctcc
     2461 ggtgttgatg ccgatccggg agtacaccgg cggcagtcct gccgccgcaa actccttccg
     2521 cagctccgcc aggcggtgct ggttgtcgag ggccgcatag caggcccggg ccgcgtgatc
     2581 ctcctgaggg atgggcgcac cccagaaggc catgatggcg tcgccctcgt acttgtctat
     2641 caatcctcca ctctccatga ttatatcagt catttccgtg agatagcggt tcagtaggga
     2701 gaccacttcc tcgggcttga gcttttccga aagactcgtg aacccggcga ggtcggagaa
     2761 aaaaaccgtg aggacccgtt tttcccctcc gaggcggagt ttgtcggggt tcttcagaag
     2821 gtcttcgagg agaaggtcgg acatgtaatg ggaaaacatc tgcttgatct ggcgccgctg
     2881 ccgtccctcg gttgcgtaac tgaacgtggt tgccgccgcg aacgacaaca gcaatccggt
     2941 aaggggcagg acggagtcta cccacagatt ccggctgaag gcgataacga tgagaaggac
     3001 aaggccgaca acgtagacga tgatcaggag accgagtttc caaaaacttg ccaccagcat
     3061 cacggtcagg gcggcggcgg ctgctgccgc cacggtcaac gcgaggagtg gaatggttga
     3121 gattctggtt cgaaaatcct tgcggagcag attctccacc agcgtggcgt ggatcgccgc
     3181 cccggggtag atagaactgg tcggagtagg tttcaggtcg taaaggcctg cggcggtaaa
     3241 gcccaggaaa acgattttat cccggaacag ttcagggggg tacgggggtt cgcccccttc
     3301 ctggatggcc agatgggatt gaatgacgtt gaacgccgag aaccggggga actcgcggcc
     3361 gtctccgtag tagcggagaa gaaaatttcc gtcgcttagg agcgggatgg aaattccggc
     3421 gacggagagc ctgttttcct cgaggacggt gggaagcttt tcgcccccgt aagcgaaggc
     3481 gctcaatccc agggccggca tccacttgtc tcggtagaga aaaacaagtg gcatccggcg
     3541 atagatcccg tcggcgtcgg gaggaatctc gacgttgcct aatccatagg cactccgggc
     3601 gaggacatcg acagggagaa taatcgattt gtaaggagtg cgcgtctgcg cgaggcggtc
     3661 ctccatagac agggcgaggc ggtcaatgat cagtttctcg tcggtcggct gagggcgctc
     3721 ttcacggctc aggaaaacag gaagaaaggt gcggcccttt ctcagggctt cggcgagaag
     3781 caggtcgtct tcgacaccgt atgaggagga ctccgtgaaa aggacatcaa agaccactgc
     3841 ccgggcgccc gccgccagac
//
LOCUS       DAOTMX010000154         3844 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_19014_length_3844_cov_3.560834, whole genome shotgun sequence.
ACCESSION   DAOTMX010000154 DAOTMX010000000
VERSION     DAOTMX010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19014_length_3844_cov_3.560834"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            261..1124
                     /gene="rpoH"
                     /locus_tag="P5573_05395"
     CDS             261..1124
                     /gene="rpoH"
                     /locus_tag="P5573_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HRT27528.1"
                     /translation="MSLPAESSALELYIGQINRFPILNAAEEFDLAVRYKKYNDLAAV
                     EKLVTSNLRFVVKIAHEYRNYGFKLIDLIQEGNIGLMHAVKKFNPYKGYRLISYAVWW
                     IRAYIQNYIIKSWSIVKIGTTQAQRKLFFKLNQAKKRLRHLSEKNPEFGEIAESLHVK
                     ESEIEEMDLRLSNRDLSLDATLTEDGELTYIDHLKYEGEDQETALIRKEETALVKRNI
                     AGALEKLTERERYIIQHRIMAENPVTLQDIGNRFNITRERARQIEAQALKKMRTAIAY
                     NRKEPVALLSA"
     gene            complement(1121..2470)
                     /gene="dnaB"
                     /locus_tag="P5573_05400"
     CDS             complement(1121..2470)
                     /gene="dnaB"
                     /locus_tag="P5573_05400"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HRT27529.1"
                     /translation="MKDVESFLHKVPPQNLEAEQSVLGGILLDNQALNSVMEILRVED
                     FYSEAHRKIFAAILDISEKSRPSDLVTLNDYLRDKKQIDAVGGAVYLASLVDTVPSAA
                     NIVHYSKIVKEKAILRNLIGTATDILTKSYNAGSDVDGLLDEAEHAIFEISENKIKPS
                     FSPLKDLIKDSFRTIERLYEKKELITGVPTGFEKLDEMTSGLQRSDLIIIAGRPSMGK
                     TAFALNIAQRAAIESGIPVAIFSLEMSKEQLAFRMLSSEAMVDSQRLRRGYPGETDWP
                     KLTTAAGRLAEAPIYIDDTAAISVLEMKAKARRLKAESGLGLLIVDYLQLMRAGGSRD
                     SREQEISEISRSLKALAKELNIPVVALSQLNRQVEQRTNRRPQMADLRESGAIEQDAD
                     VIMFLYRDEVYNRTEENTGLAEVNIGKQRNGPTGTVKLVFLEKFTRFENPSREYDPY"
     gene            complement(2475..2924)
                     /gene="rplI"
                     /locus_tag="P5573_05405"
     CDS             complement(2475..2924)
                     /gene="rplI"
                     /locus_tag="P5573_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HRT27530.1"
                     /translation="MKVILRQDVESLGKMGDVIRVAEGYARNYLIPKGIAVEANEKNI
                     RALEHEKKEMEQRAGKRRKKAEALLETLSGITCVISRKTGKEDKLFGAVTAKDVEKSL
                     RDQGLDIDRKSIVFQEPIKSLGEFPVKVKLYPGVTAEITVKVVKEED"
     gene            complement(2950..>3844)
                     /locus_tag="P5573_05410"
     CDS             complement(2950..>3844)
                     /locus_tag="P5573_05410"
                     /inference="COORDINATES: protein motif:HMM:NF021506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HRT27531.1"
                     /translation="VFTPLIGPVAEFFVPLPVMYYSARLGRLRVGAVFCLALFLVSVP
                     VVLFGLKINFALFFFLGLFGLVLSELFKKDLTIERTVLYGTFVILTVALLLLGYHLQR
                     TGQNPVQAVESFVRDGVDESIGAYAQLGMPAEQVGELRKSVPHVVRAILGLLPAFVVI
                     SAVTFVVLNVLVGMAFLRKRGLPARDFGDLNLWKIPDRMVWFVIAAGTLVLIPQENIR
                     LIGLNLLVVFLFAYVLQGFAIINYFFTRKRVPVFLRWMVYALIFVQNIFLLVVAVIGF
                     ADIWVDFRKINPPAADSVSQE"
BASE COUNT          963 a         1086 c          945 g          850 t
ORIGIN      
        1 aagtctcaat tctttcaagg tcttttcttt ctaaaccaat tacctcgaga aggcaaaagg
       61 tttttatccg acccgaacgg catcagcgac cctgtgcggt atgcgtggct atacggctgt
      121 ttcggaccgg gacagaagcc tggcgcagaa agcccgaata tgccttccat cgccggcatt
      181 tactcctaag attctttaac aacccttgac taacatgtta aaaatattat actgattaat
      241 cactaaacca tggaggattc atgagtctac ccgccgagtc gagcgccctt gaactttaca
      301 taggacagat caaccgtttt ccgatcctca acgccgctga ggaatttgac cttgcagtcc
      361 ggtacaagaa atataatgac ctggctgcag tggagaagct cgtaacttcg aacctgcgtt
      421 tcgtagtaaa gatcgcccac gaataccgga attacgggtt caaactcatc gacctgatcc
      481 aggaagggaa catcgggctg atgcacgccg taaaaaaatt caacccctac aagggatacc
      541 ggcttatatc ttatgcagta tggtggatca gggcgtatat ccagaattac atcataaaat
      601 catggagtat cgtcaagatc ggcacgacac aagcccagag aaagctgttt ttcaaattaa
      661 accaagcaaa gaagaggctc cggcaccttt ccgagaaaaa cccggaattc ggggaaatcg
      721 ccgagtccct gcacgtcaaa gaatcagaga tagaggaaat ggaccttcgc ctcagcaacc
      781 gcgacctctc cctcgatgcc accctgacgg aggacggtga actgacttac atcgaccacc
      841 tgaagtatga aggagaggac caggaaacgg cgctcatcag gaaggaagaa acggcgctgg
      901 tgaaaagaaa catcgccggg gcgctggaaa aactgacaga aagggaacgt tacatcatcc
      961 agcataggat catggcggag aaccctgtaa ccctacagga catcggcaat cggttcaaca
     1021 tcaccaggga aagggcccgt cagatcgaag cccaggcgtt aaaaaaaatg cgcaccgcca
     1081 tcgcatacaa ccggaaagag ccggttgccc ttctctctgc ctaatagggg tcatactccc
     1141 gggaggggtt ttcaaagcgg gtaaactttt ccagaaagac cagtttgacg gttccggtag
     1201 gcccgtttct ctgttttccg atgttcactt ccgccagacc cgtattctct tccgtgcggt
     1261 tgtatacttc atcccggtaa aggaacatga tcacgtcggc atcctgctcg atggcgcccg
     1321 attccctcag gtcggccatc tggggccgtc tgttcgtcct ctgctctacc tgccggttga
     1381 gctgcgaaag cgccaccacc gggatgttca gttccttagc cagcgccttg agggaacggc
     1441 tgatctccga gatctcctgt tccctggaat ccctggatcc ccccgcccgc atgagttgca
     1501 gataatcgac gatcaagagg ccgagccccg actcggcctt caggcgcctc gccttggcct
     1561 tcatctcgag cacggatatc gcagctgtgt cgtcgatgta gatgggcgcc tcagcaaggc
     1621 gtccggcggc agtcgtcagt ttcggccagt ccgtctcccc gggatacccc cttcggagcc
     1681 tctgagaatc caccattgcc tccgaggaca acatacggaa ggccaactgt tctttagaca
     1741 tttcgaggga gaaaatagcc acgggaatgc ccgattcgat cgcggcacgc tgggcgatgt
     1801 tcagggcaaa ggcggtctta cccatgctcg gccggcccgc gatgattatc aggtccgacc
     1861 tctgcaaccc cgaagtcatc tcgtcgagct tctcgaaccc cgtgggaacg cctgtgataa
     1921 gctccttttt ttcgtacaga cgctcgatcg tcctgaaact gtctttgatc aaatccttca
     1981 ggggagaaaa actcggcttg atcttatttt cggaaatctc gaagattgcg tgctccgcct
     2041 cgtcgaggag gccgtccacg tcggaccctg cgttgtagct ttttgtcagg atgtccgtcg
     2101 ccgtcccgat tagattcctc aggatggcct tttccttcac gattttcgag taatggacaa
     2161 tgttggccgc ggacgggacg gtgtccacaa gcgatgccag ataaacggca cctcccaccg
     2221 cgtcgatctg cttcttgtcc cgcaggtaat cgttaagggt gacgaggtcg ctaggtcggc
     2281 tcttttccga aatatccagg atggcggcaa aaatcttgcg gtgagcctcg ctgtagaagt
     2341 cctctacccg caaaatctcc atcacgctgt tcagggcctg gttgtcaagc agtatccccc
     2401 cgaggaccga ctgctcggct tcgaggttct ggggtggtac tttgtgaaga aaggactcca
     2461 catccttcat ggggtcaatc ctcttccttg accaccttga ccgtgatttc cgccgtgacc
     2521 ccggggtaga gtttgacctt caccggaaat tctcccaggc tctttatcgg ctcctgaaag
     2581 acgatactct tgcggtcaat atccaggccc tggtcgcgaa gtgacttctc tacatccttc
     2641 gcagttaccg cgccgaaaag cttgtcctct tttcccgtct tccgggaaat gacgcaggta
     2701 atgccgctca gggtttcgag caaggcttcc gctttcttac gcctcttccc ggcgcgttgc
     2761 tccatctcct tcttttcatg ttcgagggca cggatgtttt tttcattcgc ctcaaccgct
     2821 atccccttgg gaataagata atttctcgcg tatccttcgg ctacacgaat cacgtctccc
     2881 atcttcccga gggactcaac gtcctgcctg agaatcacct tcatggaaac aactccttgt
     2941 ctgacgagat tattcctgac ttaccgagtc tgccgcaggc gggtttatct tccggaaatc
     3001 gacccagatg tcggcaaaac cgatgaccgc aacgaccaga agaaagatat tctgcacaaa
     3061 aatcaaggca taaaccatcc aacgcagaaa taccgggacc ctcttcctgg taaaaaaata
     3121 gttgattatg gcaaagccct gaagaacata ggcaaacaaa aaaacgacca gcagattaag
     3181 cccgataagc cggatatttt cctgaggtat caaaacgagc gtacctgcgg cgatgacaaa
     3241 ccagaccatc cggtccggga tcttccacag attaaggtca ccgaaatcac gagcagggag
     3301 ccctcttttc cgtaggaacg ccattcccac aagcacgttc aaaacgacga aggtcaccgc
     3361 actgattact acgaacgccg gtaagagacc gaggattgcc ctgactacat ggggcacact
     3421 cttcctcaat tccccgacct gttcggcagg cataccgagc tgtgcataag ccccgatgct
     3481 ctcgtctacg ccatcccgta cgaaggattc aaccgcctgc accgggtttt gacccgtcct
     3541 ttgcagatga taacccagta aaagaagggc tacagtcagg atgacgaagg ttccgtataa
     3601 aaccgtcctt tcgatggtga gatccttctt gaacagctcc gacagaacca agccgaataa
     3661 accgaggaaa aagaacaggg caaagttgat tttcaggccg aaaaggacga cgggaaccga
     3721 cacaaggaaa agggcaaggc agaaaaccgc gcctactctg aggcgtccga gtctggcgct
     3781 atagtacatc accggcaggg gcacgaagaa ctctgcaacg ggacctatca gaggggtaaa
     3841 aaca
//
LOCUS       DAOTMX010000155         3831 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_19088_length_3831_cov_3.521981, whole genome shotgun sequence.
ACCESSION   DAOTMX010000155 DAOTMX010000000
VERSION     DAOTMX010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19088_length_3831_cov_3.521981"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..1133
                     /locus_tag="P5573_05415"
     CDS             171..1133
                     /locus_tag="P5573_05415"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HRT27532.1"
                     /translation="MVGRRTILTLGLSFLILFIGLGSAFGSEKYCVKRGDTLCKIARK
                     HGVTPQALRDANGLRNNSLRVKQTLIIPGDKSKTKKKSARAGNELPPDREIYVVKEGD
                     TIQGVADYAGLSVAELKRMNHLRTRKLRDGQRLVIPKQEAFDEMEDALSDGDEAASTP
                     PAEADEDKPASASVLGKWRSPDERKIFVRVAKTFLGVPYRLGGSSMKGIDCSAFVRKV
                     YEIFDIRLPRTAREQARVGKWVSKEELEEGDLVFFKTRSRRVTGHVGIYIGNGEFVHA
                     SGKHRGVKVDSLASEHYSKYFVRGVRVMDIDRELAKNKDQKQNL"
     gene            complement(1200..2240)
                     /locus_tag="P5573_05420"
     CDS             complement(1200..2240)
                     /locus_tag="P5573_05420"
                     /inference="COORDINATES: protein motif:HMM:NF024891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HRT27533.1"
                     /translation="MNILIFGSMCQSTGKTSLIVGLARALNKKFGYAKPFGDRLLYRK
                     KRLWDYDAALITNIFGLHEIPDDISIGFDHSKLRFMYDEKAIQEKLRNVISDVGNDRD
                     FVFIEGGKEINYGISVYLDPLSVAAYTGGKLIIVVSGNENAVMDDLIFLKKRIDITGV
                     DLGGIIINKIQNIDEFKENNLAKIEETGLKVLGMIPFEKNLTTFTVGYLAERLFAKVV
                     TGEEHMEKTVKSLFIGAMHVNAALESPLFKEEGKAVITSGDRTDMILAALEGDTACVV
                     LTDNITPNAKIISAAKERNIPLLMVPADTFQTAKQLDLMEPLLTKDDTEKVELLKNTV
                     LQYVKIDELIEK"
     gene            complement(2278..>3831)
                     /locus_tag="P5573_05425"
     CDS             complement(2278..>3831)
                     /locus_tag="P5573_05425"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HRT27534.1"
                     /translation="DEADLLEYLVPQDRTKIILMYIEGVKDGEKLAGQLRTATAKKPV
                     IVIKSGRSAKGAMAAASHTGSLAGSDEVFDHVMRQCGVLRAESLREAFDWCNFFSNNP
                     LPKGENTLIITNGGGIGVMAADACEKYGVQLYDDTRRLKDVFASVVPAYGSSKNPVDL
                     TAEASSDDYDAAFEMALNIDSVHAVLGLYCETAVFDKENLAEMVSRNDARLKAGGKPA
                     SFSLLGGESTVRDIETLEKQGVTVVDDVYQSVSPLGAMFFYHRYLHDQPGPPVTTPVD
                     MAVVDAVVSRAMKEGRTFLFSQEAQLLIKSAGIAVPKSGVARTIEEAVKLAKEMGYPV
                     VMKVVSKDILHKSDAGGVMLDLLNEEEVVNAYQAIVHNSLSAVPNAVIEGIEVAEMVS
                     PGTEMIAGSRRDKIFGPIAMVGLGGIYVEVIKDVTFRALPLDRKEVIKMIKELKTYPL
                     LLGVRGEKTKDQDALVDTTIRLGSIIRQCPHISDIEINPLMVYEQGMGVKAVDIRILL
                     SKQAKGINL"
BASE COUNT          807 a         1020 c         1101 g          903 t
ORIGIN      
        1 ggggtgtcaa atgaaagaaa aagagtgagg aaaaaggttg acaaggaagg gcgagtttta
       61 tattaggaag gcacacatca aataacccac gtccggtttg attttcgttt cagtttcagc
      121 atcgaaacgg aaatcgccct gtcatgatcg aaaccgagga gggaaccctt atggtgggac
      181 gacgtacgat tttgacgctg gggctgtctt ttcttattct tttcatcggt ctcggcagcg
      241 ctttcggcag cgagaagtac tgtgtcaaga ggggtgatac tctttgcaag atagcccgta
      301 agcatggagt aacccctcag gccttgagag acgccaacgg actccggaac aactcccttc
      361 gggtaaaaca gacgctcatc atcccggggg ataagtcgaa gacaaaaaag aaaagcgcca
      421 gggctgggaa tgaactcccc cccgaccggg aaatctatgt cgtcaaagag ggggatacga
      481 ttcaaggtgt ggccgactac gcaggtctgt cggtcgcgga gctgaagagg atgaaccatc
      541 tgcggacgag gaagttgagg gacgggcaga ggctcgtgat cccgaagcag gaggccttcg
      601 acgagatgga ggacgcccta agcgacggcg atgaggccgc ttccacccct ccggcggagg
      661 ccgacgagga caaaccagca agcgcgtcgg tcctcggaaa gtggaggagt cctgacgaga
      721 gaaagatatt cgtcagggtg gctaagacat ttctgggcgt accctatcgt cttggcgggt
      781 cgagcatgaa ggggatcgac tgctcggcct tcgtcaggaa ggtctatgaa atcttcgaca
      841 tccggctccc ccggacggcc agagagcagg cccgggtcgg caagtgggtt tcgaaggaag
      901 agttggaaga gggggacctt gtgttcttca agacccgctc ccgccgcgtg acgggtcacg
      961 tgggcatcta catcgggaac ggtgagttcg tccatgcctc gggcaagcac aggggcgtca
     1021 aagtcgacag tctggcgtcc gaacattaca gcaagtactt cgtcagaggt gttcgggtga
     1081 tggacatcga cagggagctt gccaagaaca aagaccagaa gcagaaccta tagggttaaa
     1141 gtaacgtaga agtccgtcgc ctttgtccgc ggcctgtaag agccgccctg cagaaaaagt
     1201 cacttttcga ttagctcgtc tatcttcaca tactgtaata cagtgttttt caggagttcg
     1261 accttctccg tgtcatcttt cgtgagcaac ggctccatga ggtcgagctg tttcgctgtc
     1321 tggaacgtgt cggcggggac catcaggagg gggatgttgc gctctttggc agcggagatg
     1381 atcttggcgt tgggggtgat gttgtccgtc agcactacgc aggccgtatc cccttccagc
     1441 gcggcaagta tcatgtccgt gcggtcgccg ctcgttatga ccgccttgcc ttcttctttg
     1501 aagagggggc tttcgagggc tgcgttcacg tgcatggcgc cgatgaacag gcttttcacg
     1561 gttttttcca tatgttcttc gccggtgacg accttggcga agagcctttc agcgagataa
     1621 ccgacggtaa aggtcgtgag gttcttttcg aagggtatca tcccgagaac cttcaatccc
     1681 gtctcttcta tcttggcaag gttgttctcc ttgaactcat ctatattctg aattttgttt
     1741 attatgatgc cgccgaggtc gacgcccgta atatcgatgc gtttcttcag gaagatcaag
     1801 tcgtccatca ccgcgttttc atttccgctc acgacgatga tgagctttcc gcctgtgtaa
     1861 gcggcgacag acagcgggtc caggtagacg gagatgccgt agttgatttc cttgccccct
     1921 tcgatgaaga cgaagtctct gtcgtttccc acgtccgaaa tcacgtttcg caatttctcc
     1981 tggatggcct tttcgtcgta catgaagcgc agtttggagt ggtcgaaacc gatggagatg
     2041 tcgtcgggaa tctcgtggag gccgaaaatg ttcgtgatga gggccgcgtc gtaatcccac
     2101 agccttttct tccggtagag caaccggtct ccgaagggct ttgcataacc gaacttctta
     2161 ttcaacgccc gggcaagccc gacgatcaag ctggtcttac ccgtactctg acacatggac
     2221 ccgaagatga gtatgttcat tctggcacct cgtaagatag aagctaacgg cacgagatta
     2281 aaggttaatg ccttttgcct gcttggacag gaggattcgg atgtcgaccg ccttcacccc
     2341 catgccctgt tcatatacca taagcggatt aatttctata tcagaaatat gcggacactg
     2401 ccggatgatg gagccgagcc ggatggtggt atcgactaag gcgtcttgat ccttcgtttt
     2461 ttccccgcgg acgcccagga gaagggggta ggtttttaat tccttaatca ttttgatgac
     2521 ttccttccgg tcgaggggaa gtgcccggaa ggtcacgtct ttaatgactt ccacgtagat
     2581 tcccccgagg ccgaccatcg cgataggccc gaagatcttg tctcgccgtg agcctgctat
     2641 catctccgtt cccggcgaca ccatctccgc tacctcgatt ccctctatca cggcattcgg
     2701 gacggcgctc agcgagttgt ggacgatggc ctggtaagcg ttgacgacct cttcctcgtt
     2761 gagcaggtcg agcatgacgc cgccggcatc gctcttatgg aggatgtcct tcgaaaccac
     2821 cttcatgacg acgggataac ccatttcctt agcgagtttc accgcctcct cgatggtcct
     2881 tgctacgccg ctcttgggaa cggctatccc cgcgctcttt atcaggagct gagcttcctg
     2941 agagaagagg aacgtccgtc cttccttcat cgccctgctg acgacggcgt ctacaacagc
     3001 catgtccacc ggtgtcgtga ccggggggcc cggttggtca tggagatacc ggtggtaaaa
     3061 aaacatggcg ccgaggggcg agacggattg atagacgtcg tcaacaacgg tgaccccctg
     3121 tttttcgagt gtctcgatgt cgcggaccgt actctccccg ccgagaaggg agaaagaggc
     3181 cggtttcccc ccggccttca accgggcgtc gttccggctc accatttcag caaggttttc
     3241 cttatcgaag accgccgttt cgcagtagag gccgaggacg gcgtggacac tgtcgatgtt
     3301 gagcgccatt tcgaaggcgg catcgtagtc gtcggacgac gcctctgccg tcaagtctac
     3361 ggggttcttc gacgaaccgt atgcggggac gacggaagca aagacgtcct tgagccttcg
     3421 ggtgtcgtcg taaagctgga cgccgtattt ttcgcaggca tcagccgcca tgacgccgat
     3481 gcccccgccg ttggtgatta tcagcgtgtt ttcccctttt gggagggggt tgttggaaaa
     3541 gaagttgcac caatcgaagg cctctctgag gctctcagcc ctcaggacac cgcactgtct
     3601 catcacgtgg tcgaagacct cgtccgaccc ggcgagcgag cccgtatgcg atgccgccgc
     3661 cattgcgcct ttcgcagacc tcccggactt gatgacgatg accggttttt tcgccgttgc
     3721 tgttctcaat tgccctgcca atttctcgcc gtccttgacg ccttctatgt acatcaggat
     3781 tattttcgtc cggtcttgag ggacgagata ttcgaggagg tccgcttcgt c
//
LOCUS       DAOTMX010000156         3818 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_19164_length_3818_cov_3.357162, whole genome shotgun sequence.
ACCESSION   DAOTMX010000156 DAOTMX010000000
VERSION     DAOTMX010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3818
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19164_length_3818_cov_3.357162"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="P5573_05430"
     CDS             <1..930
                     /locus_tag="P5573_05430"
                     /inference="COORDINATES: protein motif:HMM:NF014510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HRT27535.1"
                     /translation="MLSSYGAMVFSSSDPKEGVIMNNLSQLIINCAHDIAKEVGSRAI
                     FLYADACKDIGALELKREGFDMILVTKDGSNLPEALKKEARTITVPNLNLTRVGQMKI
                     AITKGIAGGLLKKGDKVVCLTGIPKFGYVDSVSVIDVGREFEILTAEYISDIFEGVQP
                     EAFEAVLNIALELAAQGREGRPIGTIFVIGDHEKVLQFSRQMIMNPFMGYKEEERNIL
                     NPALLDTIKEFSALDGAFILKDNGVLLTAGTHLNAAFEGKDFPKGLGSRHIAAAGITN
                     VTNAIAVVVSESSGTVRVFKKGKIFVSIEKPVE"
     gene            972..3560
                     /gene="clpB"
                     /locus_tag="P5573_05435"
     CDS             972..3560
                     /gene="clpB"
                     /locus_tag="P5573_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HRT27536.1"
                     /translation="MRFEKLTLKAQEAFQEAQRTAERLGHQAVDVEHIVLAVIKQPEG
                     TVEAVLKMLGANPGAVARDIEKMLERIPRVSGAGGQVYIAPRLSNMMENAFKEAEAMT
                     DEYVGVEHILIAAAELKQDPVGRILQSAGVTKDKILRVLVQIRGTQRITDQNPEEKYQ
                     ALKRYARDFNDLARKGAFDPVIGRDEEIRRVMQVLSRRTKNNPVLIGEPGVGKTAIVE
                     DLAQRIVNGDVPESLKEKRVIGLDMGALIAGAKFRGEFEDRLKAVLKEVTESHGEIIL
                     FIDEIHTVVGAGAAEGAVDASNMLKPALARGELRCIGATTINEYRKYIEKDAALERRF
                     QPILVKEPTVEDTIAILRGLKERYEIHHGVRISDSAIIAAATLSSRYITDRFLPDKAV
                     DLIDEAASRLRIELDSLPAEIDAVERKIIQLQIEKESLKKEESPSAREREAKIDKDLE
                     ELRATKEQMMQHWMREKDVIKSIQELKSQIEECKNEEINAQRVGDLEKAAAIRYGKLI
                     ELNRKLEEEQKRLAEVQKDKKMLKEEVDEEDIAEVVSKWTGIPVTKMLESDAQKLLHM
                     EERLKQRVVGQDEAVRLVSNALRRARSGLQDPNRPLGSFIFMGSTGVGKTELARALAE
                     FMFDDEQAIVRIDMSEYMEKHSVARMIGAPPGYVGYEEGGYLTEAVRRRPYSVVLFDE
                     IEKAHPDVFNIMLQILEDGRLTDGHGRTVNFRNTIIIMTSNIGGEFVQNFTMSEEERK
                     LRTLEALRATFRPEFLNRVDDIVFFRSLSISDIERIIEIQIDRLQKRLEDRKLTLELT
                     DRAKRYIIKVGYSPVYGARPLRRAIQKIVQDALAIKLLEGAFQEGDYIVVDITDEGEV
                     IFSKKQ"
     gene            3634..>3818
                     /locus_tag="P5573_05440"
     CDS             3634..>3818
                     /locus_tag="P5573_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HRT27537.1"
                     /translation="MNSLKTALLLGALTGLLMLIGGWIGGRQGVVIAFLFAAVMNFVS
                     YWYSDRIVLRMYGANEVT"
BASE COUNT          991 a          893 c         1198 g          736 t
ORIGIN      
        1 atgttgagtt cttatggtgc tatggtgttt agttcgtctg accctaaaga aggggtaatc
       61 atgaataacc tgagtcaact tatcatcaat tgcgcccacg acatcgctaa ggaggtcggg
      121 tcacgggcga tttttctcta tgccgacgcc tgcaaggaca tcggggcttt ggaactgaag
      181 cgcgaaggct tcgacatgat cctggtcacc aaggacggct cgaacctgcc ggaggctctc
      241 aagaaagaag caaggacgat cacggttccc aacctgaacc tgacacgggt cgggcagatg
      301 aagatcgcca tcaccaaagg gattgcaggg ggtctgctga aaaaaggcga caaggtcgtg
      361 tgtctgaccg gtattcccaa gttcggttac gtggacagcg tttccgtgat cgatgtcggc
      421 agggagttcg agattctgac ggccgagtac ataagcgata ttttcgaggg agtccagcct
      481 gaagcttttg aggccgttct caacatcgcc ctcgagctgg cggcgcaggg acgggaaggg
      541 cgtcccatag gtaccatctt tgtcatcggg gaccacgaga aggtcttgca attttcgcgg
      601 cagatgatca tgaacccctt catgggttac aaggaggagg agagaaacat cctcaatccg
      661 gcgctcttgg ataccataaa ggagttttcg gccctcgatg gggctttcat cctgaaggat
      721 aatggggtgt tactgacggc ggggacacac ttgaatgccg cctttgaggg aaaagatttt
      781 ccgaaaggcc tcggcagcag gcacatcgcc gcggcaggca tcaccaatgt gaccaatgcg
      841 atcgccgtcg tcgtctcgga atcttcagga accgtcaggg tgttcaagaa gggaaagata
      901 ttcgtcagca tcgaaaagcc ggtggaatga tgtaataaag gaattgagtg aaatgaatca
      961 ggaggatcta tatgaggttt gaaaaattga cattgaaggc ccaggaggcc ttccaggagg
     1021 cccagaggac ggcggagcgg ctcggacacc aagccgtcga tgtggagcat attgtgctcg
     1081 ctgtcatcaa acagccagag gggactgtgg aggcggtttt gaagatgctc ggcgccaatc
     1141 ccggggccgt cgccagggac atagagaaga tgctggagag gataccgcgg gtgtccggag
     1201 caggtggcca ggtgtacatc gcgccccgat tgagcaatat gatggaaaac gccttcaaag
     1261 aggcggaagc catgacggat gagtacgtcg gcgtggagca catcctgatc gccgccgcgg
     1321 aattgaagca ggacccggta ggacggatac tccagtccgc cggtgtgaca aaagataaga
     1381 tcttgagggt gctcgttcaa atccggggga ctcagcgtat aaccgaccag aatcccgaag
     1441 aaaaatacca ggccctgaag cggtacgcca gggacttcaa cgatctggcc cgtaaggggg
     1501 cttttgaccc tgtgatcggc agggacgaag aaataaggcg cgtcatgcag gtcctatccc
     1561 ggcgtacgaa aaacaatccc gttctgatcg gggagccggg cgtgggtaag acggcgatcg
     1621 tggaagacct ggcgcagcgc atcgtgaacg gagacgtccc ggaaagtctg aaagaaaagc
     1681 gcgtcatcgg gctggatatg ggtgccctga tcgccggagc gaagtttcgg ggagagttcg
     1741 aggacaggct caaggcagtc ctcaaggagg taaccgagtc ccatggtgaa atcatcctct
     1801 tcatagatga gattcacaca gtcgtggggg cgggggcagc cgaaggggct gtcgacgcgt
     1861 ccaatatgct caagcccgcc cttgccaggg gggagctgcg ctgcatcggt gccacgacga
     1921 tcaatgagta tcgcaagtac atcgagaagg atgccgctct ggagagacgg tttcagccta
     1981 tcctcgtgaa ggaacccact gtcgaagaca ccatcgccat cctgaggggg ttgaaggaga
     2041 ggtatgagat tcaccatggc gtgcggatat ccgattccgc gatcatcgcg gcggcgacgc
     2101 tctcgagccg ttacatcacc gaccgttttc tgcccgacaa ggccgtggac ttgatcgacg
     2161 aagcggcatc gcgcttgcgg atcgagctgg acagtctgcc ggcggaaatc gacgccgttg
     2221 aaagaaagat aatccaactc cagatagaga aagagtctct caagaaggaa gagagtccca
     2281 gcgccagaga aagagaggca aagatagata aagaccttga agagctgagg gcgactaaag
     2341 agcagatgat gcagcactgg atgagagaga aggatgttat caagagtatc caggagctca
     2401 agagtcagat agaagaatgc aagaacgagg agataaacgc ccagcgtgtg ggcgacctgg
     2461 agaaagcggc tgcgatccgg tacggcaagt tgattgaact caataggaag ctcgaagaag
     2521 agcagaaaag gcttgccgag gtacagaaag acaagaagat gctgaaggag gaagtggacg
     2581 aggaggatat agccgaagtt gtttccaagt ggacaggtat cccggttacg aagatgctgg
     2641 aaagtgatgc gcagaaactc ctccacatgg aagagaggct gaagcagcgg gtggtcggcc
     2701 aggacgaggc agtgaggctg gtctcgaacg ccctcaggag ggcccgttcc gggctgcagg
     2761 acccgaatcg ccccctcggt tccttcatct tcatgggttc gacgggcgtc ggcaagacgg
     2821 aactcgccag ggcgctggcg gagttcatgt tcgacgacga acaggcgata gtccggatcg
     2881 acatgtccga gtacatggaa aagcactctg tcgccagaat gatcggagcg ccgcccggtt
     2941 atgtcggcta tgaagaaggg gggtacctga cggaagcggt gcgcagaagg ccttattcgg
     3001 tcgttctttt cgacgagatc gagaaggccc atccggatgt cttcaacatc atgttgcaga
     3061 tcctcgagga cgggaggttg accgacggac acggcaggac ggtaaatttc aggaacacga
     3121 tcatcatcat gacctccaac atcggtggcg agttcgtcca gaacttcacg atgagcgagg
     3181 aggagaggaa gctgagaacc ctcgaggccc taagggcgac cttcaggccc gagttcttga
     3241 accgggtgga tgacatcgtg ttcttccggt ccttgtcgat cagcgatata gagaggatca
     3301 tcgagattca gatcgaccgg ctccagaaaa ggctcgaaga ccgcaaactg accctggagt
     3361 taaccgatag ggccaagagg tatataataa aagtcggtta cagtcccgtt tacggggcaa
     3421 gacctctgcg ccgggcgatt cagaagatag tccaggatgc actggccatt aaattgctgg
     3481 aaggtgcctt tcaggaaggc gactacatcg tggtcgatat aacggacgag ggtgaggtta
     3541 ttttcagcaa aaaacagtaa gaacggctcg ttgtaatcgg gtcttaaaat cagtataata
     3601 caccgaaaaa agaaggaggt ttttttaagc gtcatgaaca gcctgaaaac ggccttgctg
     3661 ttgggtgccc tcacaggtct actgatgttg atcggcggat ggatcggcgg ccgccaaggt
     3721 gttgtcatcg cctttttgtt cgcagcagta atgaacttcg tgagctactg gtactccgat
     3781 aggatcgtgc tgagaatgta cggggcaaat gaggtcac
//
LOCUS       DAOTMX010000157         3766 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_19428_length_3766_cov_3.264888, whole genome shotgun sequence.
ACCESSION   DAOTMX010000157 DAOTMX010000000
VERSION     DAOTMX010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3766
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19428_length_3766_cov_3.264888"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="P5573_05445"
     CDS             <1..693
                     /locus_tag="P5573_05445"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRT27538.1"
                     /translation="GSGTIFMTHCNLRCIFCQNYSISRLGEGYRITFEKFSRVMVELE
                     QMGCHNINFVSPSHFVPHILKALPRAVEMGLSVPLVYNTGGYDSVETLKLLDGVFDIY
                     MPDFKYTKNDVAEKYSGAPDYPEVVMAALKEMHRQTGDLEMDEDGIALRGLLVRHLVL
                     PEGLAGTREAMRFLAREISRNTYVNIMDQYHPCGDLIPPGSPLSRRITRKEFMEAIEI
                     AREEGVTRLDRG"
     gene            695..1966
                     /locus_tag="P5573_05450"
     CDS             695..1966
                     /locus_tag="P5573_05450"
                     /inference="COORDINATES: protein motif:HMM:NF020048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRT27539.1"
                     /translation="MRKKGGIFVYLRIDRDEPYPHLEVTRLVRKDGALYFGPYPVTRA
                     LHETLRLINRHFHLRTSSDHDPRQHKRPCLLCRISRLPDPAPCDIPQEQYREQVEDAV
                     SFLEGRKSELLASLKERMAREAAELRFEEAARLRDRIGAVERTPEPRKVESDLLSIDE
                     DGIGPHLEDDMSRLLERLRHRLQLSRAPNRIECFDVSHLVGEMTVASRVAMMEGKLDR
                     DRYRRYRIRTAGRGDDCAALFEAVSRRLRKGLEENDLPDLIVLDGGKPQLSAGLAALQ
                     ALGVADVDIVALAKTRTVEPEMPGEGPVKVSERIYIPEKTAPVVLPHTSPEMLLLVRL
                     RDEAHRFAVTYQDKLSRKERLRSELDAIPGVGEKRKKALLRRFGSVEEISKAGVEEIS
                     KTEGMGLETARTVYAFFHDVVPDQETEKDGP"
     gene            1956..>3766
                     /locus_tag="P5573_05455"
     CDS             1956..>3766
                     /locus_tag="P5573_05455"
                     /inference="COORDINATES: protein motif:HMM:NF036484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27540.1"
                     /translation="MARSPIRVEGARQNNLKDITVEVPVGAVTVITGVAGAGKSSLAF
                     DVLYAEGYRRYVETFSPYARQFLERLDRPKADRIEGILPGIAIERAAPVKTSRSTVGT
                     MTSVDDYLRPLFARNATLYCRSCNLPVFRHSPASIFEALLKTAPGRQAFICFTRPIGS
                     ADPQTVRDVFRQAGFLRVLEDGKAVRVDEARLQSNDGKITVVLDRVTVDAEARQRVVD
                     SVETALKFGEGSLEVLIEGEARPLRFSRKLHCERCDIDYNEPTAALFSFNNPIGACEN
                     CHGFGRVIDIDPDLVIPDGGLSLSAGCVRPFQTASFRECQRDLLDFARRRRIPTTTPW
                     HDLDHDTRKAVWDGEGDWYGIQGFFDWLMGRRYKKQARILLSRYRRFLPCPVCGGGKL
                     KPDALLFRLDGKTFPELEEMAVSAVESFFRRWSPPAEDRASELLLGELSGRLGFLLDV
                     GLGYLSLGRLSRTLSGGETQRVTLATALGSSLTGTLYVLDEPSVGLHPRDAERLAGVL
                     RTLASAGNAVVVVEHDPVFIRKADRVIDLGPGPGERGGSVIHQGSVTGLIGNRRSPTA
                     AYLRGEISMPQPSRKRTPHRGSLRVIGARENNLKNFSV"
BASE COUNT          743 a         1238 c         1132 g          653 t
ORIGIN      
        1 gggtcgggta ccatcttcat gacccactgc aacctgcgtt gcatcttctg ccaaaattac
       61 agcatcagcc gtctcggtga agggtacagg attactttcg agaagttcag cagggtcatg
      121 gtggaactcg agcaaatggg ctgtcacaac atcaacttcg tgagtccctc tcacttcgtt
      181 ccccacatcc tgaaggccct gccgagggcc gtcgagatgg ggctttccgt cccgctggtc
      241 tacaacacgg gagggtacga ttcggtggag accctcaagc tcctcgacgg cgtcttcgat
      301 atctacatgc ccgatttcaa gtatacgaag aatgatgtgg ccgaaaagta ttcgggggcg
      361 cctgattatc ccgaggtagt tatggcggcc ttgaaagaga tgcaccggca gacgggcgac
      421 ctcgagatgg acgaagacgg gatcgccctc cggggtctcc tggtcagaca cctggtcctc
      481 cccgaaggcc ttgccggtac ccgtgaggcc atgcgcttcc ttgcccggga gatctcacgg
      541 aacacctatg tcaacataat ggaccagtac catccctgtg gcgacctcat cccgccggga
      601 tcgcccctct ccaggcgaat cacaagaaaa gagttcatgg aggcaataga aatcgcgaga
      661 gaggaaggcg tgacgcgcct cgacagggga tgagatgaga aaaaaaggcg gcatctttgt
      721 ctatctacgc atcgacaggg acgaacctta tccgcatcta gaggtgactc gcctcgtaag
      781 gaaagacggc gccctctact tcggccccta cccggtgacg cgcgccctgc acgagaccct
      841 ccgcctcatc aaccggcact tccatctgcg gacctcttcc gaccacgacc cgcgacagca
      901 caaacggcca tgcctcctct gccggatcag ccgcctgccc gaccccgcgc cctgcgacat
      961 cccgcaggaa caataccgcg aacaggtgga agacgctgtc agcttcctcg aaggcaggaa
     1021 gtccgaattg ctcgcctcgc tgaaggagcg catggctcgg gaggcggcgg agctccgttt
     1081 cgaagaggcc gcacgcctgc gcgaccggat aggtgccgtg gagcgcaccc ccgaaccccg
     1141 aaaagtcgaa tctgacctgc tatccatcga cgaagacggc atcggccccc accttgagga
     1201 cgatatgtcc cgtctcctcg aaagactgcg gcaccgtctc cagttgagcc gcgcccccaa
     1261 ccgcatcgaa tgcttcgacg tctcccatct cgtcggggaa atgaccgtcg catcaagggt
     1321 cgccatgatg gaggggaagc tcgacagaga ccgttaccgc cgttaccgga tcaggacggc
     1381 gggccgcgga gacgactgcg cggccctgtt cgaggccgtg agtcgccgac tgaggaaagg
     1441 actggaggag aacgacctac ccgacctgat cgtcctggac ggcgggaaac cccagctttc
     1501 cgcggggctt gccgccttgc aggccttggg ggtcgcggat gtggacatcg tcgccctggc
     1561 gaaaacgcgc acggtcgaac cggagatgcc gggggaagga ccggtaaagg tatcggagcg
     1621 catctacatc ccggagaaaa ccgcgcccgt cgtcctgccg catacatccc cggagatgct
     1681 gctcctcgtc cgactcaggg acgaggcaca ccgtttcgcc gtcacctacc aggacaagct
     1741 cagtcgcaag gaaaggctgc gctccgagct cgacgccatc cccggtgtcg gggaaaagag
     1801 aaaaaaggcc ctcctgagac gcttcggctc cgtagaggag atatcgaagg ccggcgtgga
     1861 agagatctcg aagacggagg ggatggggct ggagacggca aggacggtct acgccttctt
     1921 ccacgacgtc gtcccggatc aagagacgga gaaagatggc ccgtagtccg atccgcgttg
     1981 aaggggcgag gcagaacaac ctgaaggaca tcaccgtcga ggtgcccgtg ggggccgtca
     2041 cggtcatcac cggggttgca ggcgccggta agtcctccct cgccttcgac gtcctctacg
     2101 ccgaaggtta tcgccggtac gtcgaaacct tttctcctta cgcgcggcaa ttcctggagc
     2161 ggctcgaccg ccccaaggcc gaccggatcg agggcatcct ccccggtata gcgatcgagc
     2221 gcgccgctcc cgtcaagacc tcgcggtcga ccgtcggcac gatgacctcc gtcgacgact
     2281 acctgcgccc tctgttcgcg aggaacgcaa cgctctactg cagaagctgc aaccttcccg
     2341 tcttccgtca ttcccccgcg tcgatcttcg aagccctctt gaagacggcg ccgggcagac
     2401 aggccttcat ctgctttacc cgccccatcg gaagcgccga ccctcagacg gtccgggacg
     2461 tcttccgaca ggcaggtttc ctccgcgtct tggaagacgg aaaggcggtc cgcgtcgatg
     2521 aggcgcggct tcagtccaac gacgggaaga tcaccgtcgt cctcgaccgc gtgaccgtgg
     2581 acgcagaggc ccgacaacgc gtcgtggatt ccgtcgagac ggccttaaaa ttcggcgagg
     2641 gaagcctgga agttctcatc gaaggggagg cgcggcccct gcgtttcagc aggaagctcc
     2701 actgcgaacg atgcgacatc gactacaacg aaccgacggc ggctctcttt tcattcaaca
     2761 accccatcgg ggcctgcgag aactgccacg gtttcggcag ggtcatcgac atcgaccccg
     2821 acctggtcat ccccgacggc ggcctcagcc tgtccgccgg ttgcgtcagg cctttccaga
     2881 cggcgtcttt ccgcgaatgc caacgggacc tcctcgactt tgcccggcgg cgccgtatcc
     2941 cgacgacgac accctggcac gacctcgacc acgacacccg gaaggcagta tgggacggag
     3001 agggagattg gtacggcatc caggggttct tcgactggct gatgggtcgc cgctacaaga
     3061 aacaggcccg gatcctcctg tcgcggtacc gtcgcttcct tccctgccct gtgtgcgggg
     3121 gcggaaagct caagcccgat gccctcctct tccgcctcga cggcaagacc ttccccgaat
     3181 tggaggaaat ggcagtctcc gcggtggaat cttttttccg ccgatggtct cctccggcag
     3241 aggaccgggc gtcggaactg ctccttggtg aactcagcgg gagactgggg tttctcctcg
     3301 atgtcggcct cggctatctc tccctcggcc ggttgtcgcg aaccctttcc ggcggagaaa
     3361 cccagcgggt gaccctcgcc accgccctcg gctcgtcgct caccggcaca ctttacgtcc
     3421 tcgacgagcc ttccgtcggg ctccaccccc gcgacgcgga gaggctggcc ggcgtgttgc
     3481 gcacgctcgc ctcggcggga aacgccgtcg tcgtcgtcga gcacgacccg gttttcatca
     3541 ggaaggcgga ccgggtgatc gacctcgggc cgggtccggg ggaaaggggc ggttccgtga
     3601 tccaccaggg cagcgtcacg ggactcatcg gcaatcgtcg ttcgccgaca gccgcctacc
     3661 tgaggggaga aatctccatg ccgcagccct cccggaaacg gacgccgcat cgaggatctc
     3721 tgcgcgtcat cggcgcccgc gaaaacaacc ttaagaactt ctccgt
//
LOCUS       DAOTMX010000158         3762 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_19447_length_3762_cov_3.864850, whole genome shotgun sequence.
ACCESSION   DAOTMX010000158 DAOTMX010000000
VERSION     DAOTMX010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3762
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19447_length_3762_cov_3.864850"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..974)
                     /gene="rmuC"
                     /locus_tag="P5573_05460"
     CDS             complement(<1..974)
                     /gene="rmuC"
                     /locus_tag="P5573_05460"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HRT27541.1"
                     /translation="MEYILCTLAGLLVGGLVAWLVASTRVSKVLSGKIEEAERQAGTA
                     EGRSLALEGTIAELRRQNEKATGELAELRNQLAAVTSARVKAETQLAETMQRLEEEKK
                     LLEEAKSRLADTFKALAGDTLNASTQAFLQLAKETLDKVLTDARGDLGKRQEAIQGMV
                     KPLAESLAKFEEHVRTVEKERQEAYSGLTEHLRILTTSQQQLQKETANLVTALRKPQV
                     RGRWGEMTLRRVVELAGMSEHCDFSEQVTVDGEGGRLRPDLIVRLPAGREIVVDAKVS
                     LEAYLDAVAAESEEARKTALTRHAAQVRSHMNILAEKQYWQQFTKAPE"
     gene            complement(1082..1714)
                     /locus_tag="P5573_05465"
     CDS             complement(1082..1714)
                     /locus_tag="P5573_05465"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HRT27542.1"
                     /translation="MKIVSIIGSPHGMRGNTGKLLGEVVKGAKSAGAVCEVIALRGDS
                     VKPCLGCDRCHKKGRCPQKDEFESIKEKLFAADGIILATPNYIFHVSAQLKMFIDRCS
                     SLIHCMALEGKYGASVVTSGGGDEVPIVEYLDYVLMVMGATPVDAVWATMGTTGTKKQ
                     LPENCREQAFALGKTLVKAWEEKWRFAKAEKVQEKHRERMRELMDYYRDE"
     gene            2104..3726
                     /locus_tag="P5573_05470"
     CDS             2104..3726
                     /locus_tag="P5573_05470"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRT27543.1"
                     /translation="MDEGKNNQILSHVYRISALLTEPTTTDRVLESIVQTVKKDLNFN
                     RCSVYLVDRATNCLECKYITGFSPHLEKFVMERPFNLARHECMETKVATTGEPILIKN
                     FNSDPSVTELDRKVTKRMGRGCTLYVPLKIKGDVIGILGVDKKHGEPEIDDDEFESLS
                     ILANYASIIIENSKLLQDLVNEKKFSENVLKSSVNGILTVDIEGRITSLNPAVEEAFQ
                     ANKEDMLKRFIWDVFDSPEWVKNTKNIVAKHVNIKGWEFALKTKGQDEAILNISTSHI
                     TNEAGSLIGFTFVIQDVTNEKRRDHYLQRVNRLVSLGELAAGVAHEIRNPLTGIGVVL
                     DILGKKRRSKAELELINDAKSEIVRLEKLISDLLTFARPRDLNFDLVDLRTVITSIFS
                     LVKEQCDQKKIKLVIKVSDDIGRLFLDRERIKQGLLNIVINAINAMPDGGELTIEAVN
                     CKNETDSGAAVKIVVSDTGTGISNENKNRIFDPFFTTSNEGTGLGLSITHSIVKEHDG
                     SIRVESEEGRGTEFTIILPCRQPRHLGVPAGL"
BASE COUNT          836 a         1026 c         1018 g          882 t
ORIGIN      
        1 aactcggggg ccttcgtgaa ctgctgccag tactgttttt cggcgagaat gttcatatga
       61 ctacgcacct gtgcggcatg tctcgtgagg gctgtcttgc gggcctcttc cgattctgca
      121 gcgacggcgt cgagataagc ctctaaggat actttggcat ccacgacgat ttcacgtcct
      181 gcgggcagcc gtacgataag gtcaggccgc agtcggccac cttccccgtc cacggtcacc
      241 tgttcggaga aatcgcaatg ctcggacatc cctgccagtt ccacgacccg gcgcagcgtc
      301 atctcacccc agcggccccg cacctgtggc ttgcgcagcg ccgttaccag gttcgccgtc
      361 tctttctgca gctgttgttg actggtggtc aaaatccgca gatgctccgt tagcccggag
      421 taggcctcct gtctttcttt ctcgaccgtg cggacatgtt cctcgaactt ggcaagcgac
      481 tcggcgaggg gcttgaccat gccttggatg gcctcctgcc gtttgccgag gtcgccacgg
      541 gcgtccgtca acaccttatc gagcgtttct ttagcaagtt gcagaaatgc ctgcgtgctc
      601 gcgttgaggg tatcaccggc aagggccttg aaggtgtccg cgagtcggct cttggcttcc
      661 tccaggagtt tcttctcttc ttcaaggcgt tgcatggtct ccgccagttg ggtctcggcc
      721 ttcaccctgg cgctcgtcac agcggccagt tgattcctaa gttcagcaag ctccccggtg
      781 gccttttcgt tctgcctgcg cagctccgca atcgtccctt ccaaggcgag ggaacgacct
      841 tcggccgtgc cggcctggcg ctcggcttcc tctatcttcc cggagaggac ctttgagacc
      901 cgcgtggatg cgaccagcca ggcgacaagg ccgccgacaa gtaaaccggc taatgtgcaa
      961 aggatatatt ccatctttat cccgcctcct tgggttaagg gctgtgcggc ggtggttcct
     1021 tccgcacatc cctcggctta gtcagggggg gccgcctctg ccagtgggcg tattcataag
     1081 gtcactcgtc gcgataataa tccatcaact cccgcatcct ttctcgatgt ttctcctgga
     1141 ccttctccgc cttcgcaaaa cgccacttct cctcccaggc cttgacgaga gtcttgccca
     1201 gggcgaaggc ctgctcgcgg caattctcgg gaagttgctt tttagtgccc gtggttccca
     1261 tcgtcgccca tacggcgtcc accggggttg cgcccatgac catgagaacg taatcgagat
     1321 attcgacgat ggggacttcg tcacccccgc cggaggttac cacactcgcc ccgtatttgc
     1381 cctcgagggc catgcagtgg atgaggctgg agcagcggtc gatgaacatc tttaattgtg
     1441 cgctcacatg gaagatatag ttgggtgtcg ccaggattat cccgtctgcc gcgaacagct
     1501 tttcctttat agattcgaac tcgtctttct gcgggcagcg gccttttttg tggcatcggt
     1561 cgcatcccag gcagggttta acggaatcgc cccgcagagc gatgacctcg cagacggctc
     1621 ccgcgctttt tgcacctttc acgacctctc ccagcagctt ccccgtgttt cccctcatcc
     1681 cgtgcgggct tccgatgatg ctcacaatct tcatggatta tcctccttct ttgctgcttc
     1741 aggcatagac ccgccgttct cgtttcctgg cccggctatt caccggatcg gcgcgctttc
     1801 cttaagttgc ttgccacagc ttacccccgg cttcctcaac gatcttgtct tcctgtttgg
     1861 acgaacgact gtcaaagtac cacagtacgt tttccattaa aagaacaatc tgccatattt
     1921 ataaggggtt actcatcgtg aggtaaggct catggccgag gtccaggaga accgttttgt
     1981 cccggcatcg gcagccgggt gtcgcaaatc acgtataagt atggtatcgg tgaataaaat
     2041 cacgatgcct ccctcgatga agggaacggg tgtatcgttt ttcacttaag aagggtaagg
     2101 ccgatggatg aaggcaaaaa caaccagata ttgtctcacg tctacaggat aagcgcgctc
     2161 ctgaccgagc cgactacgac ggacagggtg ctggaatcta tcgtccagac tgtcaagaag
     2221 gacctaaatt tcaacaggtg ctctgtttac cttgtcgata gagctacgaa ctgtctggag
     2281 tgcaaataca taacgggctt ttctccccat ctggagaagt ttgtgatgga gaggccgttt
     2341 aatcttgcaa ggcatgaatg tatggaaacg aaggtggcga cgacggggga gcccatcctt
     2401 atcaaaaact tcaattctga tcccagcgtg accgagctcg acagaaaggt cacgaaaaga
     2461 atgggccgcg gttgcacatt gtacgtgccc ttgaaaataa agggagacgt catcggaata
     2521 ttgggggtgg acaagaaaca cggtgaaccc gagatcgatg acgatgaatt cgaatcccta
     2581 tcgatcctcg ccaactacgc cagcatcatc atcgaaaatt cgaagcttct gcaggacttg
     2641 gtgaacgaga agaagttttc ggagaatgtc ctgaagagtt ccgtcaacgg aatcttgacg
     2701 gtcgatatcg aggggcggat cacctctttg aacccggcgg tcgaggaggc gtttcaggcg
     2761 aataaggagg atatgctcaa aaggttcatc tgggatgtgt tcgactcacc cgaatgggta
     2821 aaaaatacta aaaacatcgt cgccaagcac gtcaacatca aaggttggga atttgcccta
     2881 aagacaaaag gacaggatga ggcgattctg aacataagca cctcccacat caccaacgag
     2941 gcgggcagtc tgatcggatt cacgttcgtg atccaggatg tgacgaatga aaaaaggcgg
     3001 gaccactatt tgcaacgggt aaaccggttg gtctccctgg gagagcttgc ggcgggtgtc
     3061 gcccatgaga tacgcaaccc tctgacgggt atcggggttg ttctggacat cctgggcaag
     3121 aagagacgtt ccaaggcaga actcgaattg atcaacgacg ccaaaagcga gatcgtgagg
     3181 ctcgaaaagt tgatttcaga cttattgact tttgcgcggc ctagggacct gaatttcgac
     3241 ctcgtggact taaggaccgt cattacgagc attttctcct tggtcaagga gcagtgtgac
     3301 cagaagaaga taaagttagt catcaaggtc tcggacgaca ttggacgttt gtttctcgac
     3361 cgagagagga tcaaacaggg gttgttgaat atcgtcatca atgccattaa cgcgatgccg
     3421 gacggcggtg aactcacgat cgaggctgta aattgcaaaa atgaaaccga cagcggggct
     3481 gccgtcaaga tcgtcgtcag tgacacgggt accggtatct ccaacgagaa caagaatcgc
     3541 atcttcgacc ccttcttcac gacgagcaat gaaggaacgg gtctagggct ttctataacc
     3601 cacagtatcg tcaaggaaca cgacggttct atccgggtag agtctgaaga aggtagaggg
     3661 acggaattta cgatcatttt gccctgcagg cagccccgcc atttgggggt cccggccgga
     3721 ttgtgaacgg gagcgaacga agatgaaaac catcctcata at
//
LOCUS       DAOTMX010000159         3756 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_19487_length_3756_cov_3.251554, whole genome shotgun sequence.
ACCESSION   DAOTMX010000159 DAOTMX010000000
VERSION     DAOTMX010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3756
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19487_length_3756_cov_3.251554"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="P5573_05475"
     CDS             <1..261
                     /locus_tag="P5573_05475"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HRT27544.1"
                     /translation="FLLSIPAILGAAAVKARPLIAMVNSGTGVGSKELALGAVAAALS
                     GYLAVRFMLWIIKEKSLRGFAWYVWALGVLIISDQVVFHRFF"
     gene            324..1127
                     /locus_tag="P5573_05480"
     CDS             324..1127
                     /locus_tag="P5573_05480"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HRT27545.1"
                     /translation="MPANENDKSREPEKQGKERVGTDVHPYLRVALLVSILVLATYLF
                     FHFGLHVYFIDRMKGLSFVKSFGPLSVLIFILLQVLQVLIAPLPGEATGIIGGYLYGP
                     VMGTIYSTIGLTIGSWLAFALSRWLGLPFVEKVVDRKILQKYDRFMERQGALVTFAMF
                     LIPGFPKDALCYIMGLSHMRTSLFLLVSTAGRLFGTVLLSVGGSCIRNDQTKTLYAIL
                     AFTAVVLVFAYLYRDKLLKLLGKKQPPLDSGKVGPDTLPDKSPDKSMDK"
     gene            1226..3712
                     /locus_tag="P5573_05485"
     CDS             1226..3712
                     /locus_tag="P5573_05485"
                     /inference="COORDINATES: protein motif:HMM:NF025038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRT27546.1"
                     /translation="MTNKKIYFEEVPVEKLRWRCNPESLKFQTTEECEYKEGIIGQDR
                     AVRALKLGLTIESPGYNIYVSGRTGTGRTSTIMRILNKLDSGKGIPKDICYVNNFKNP
                     DMPKVIFLPAGMGQKFARDMDDLIDHLIKQIPLIFESDVFKKASEEIIESYRSKQKEM
                     MKEFNDRILKDGFQLVAFQIGPFTRQDIVPVYEGKPVPFEQLEALAEQGNFSKEELER
                     IKKKLLDYRIELDSLMREMRENEKKIRKEIDGLEYRSGLPVVSELISDIRVKYSDDNA
                     KINEYLDEVQEHILSNLKMFKEKEEEQQPHPPIPLPFVAVPQPRFTEFKVNVLVDNSQ
                     TKKTPVILETDPTYKNLFGTIEREVDRAGFWRTDFTKIKAGSLLRANGGYLVFMALDA
                     LIEPGVWYTLKRTLKNRSLNMQPFDPYGIMPTALKPEAIPLDVKVVMVGDDYIYRLLY
                     VFEEDFKKIFKTKSDFDTEMPNEEKHVDDYVCFIKRIIDDEKLLQFDRAAVASIIEHG
                     VRLTGRQKKLSTRFSEITDLIRESHYWARQEGSALVTDKHVEKAYEEKVSRVNLIEDK
                     IQEMIEDGVIMIDTEGAVVGQINGLSVYDLGDHLFGKPSRITAKTSIGRAGVINIERE
                     AKLSGKTHDKGVLILEGYFRGKYAQDKPLTMSASLCFEQSYSGVDGDSASSTEVYAIM
                     SSLANLPIRQDLAVTGSVNQKGEIQPIGGVNYKVEGFYDVCRAKGLTGTQGVLIPALN
                     VPELMLRKDVVEAVRQGRFHIYPVRTIDEGIAILTGVEAGERDETGQYPENTVNRMVD
                     ERLKNIALSLKTFGEEEKKPGQGQAPEPSA"
BASE COUNT          945 a         1032 c         1034 g          745 t
ORIGIN      
        1 tttctcctct ccataccggc gatcctggga gccgccgccg tgaaggcaag acctctgatc
       61 gccatggtca attcgggaac cggggtgggg agcaaagagt tggccttggg cgctgtcgcg
      121 gccgccctgt ccggttactt ggccgtacga ttcatgctat ggatcataaa agagaaaagc
      181 ctgcgaggct tcgcctggta tgtctgggcc ctgggggttt tgatcatctc cgaccaggta
      241 gtctttcatc gttttttttg agaaaacaca gggctttcgg tcctttacag gctcggcccc
      301 tatattatca ctgaatttac atcatgcctg ccaacgagaa cgataagagt cgggaacctg
      361 agaaacaggg caaagaacgg gttggcaccg atgtccaccc ctacctgcgg gtcgccctct
      421 tagtctcgat ccttgtcctg gcaacctacc ttttcttcca cttcggcctt catgtatatt
      481 tcatcgacag gatgaaaggc ctttcctttg tgaagtcatt cggcccctta tcggtcctga
      541 ttttcatcct cctgcaggtc ctccaggtcc tcatcgcacc gctacccggc gaagcgacgg
      601 ggatcatcgg aggctatctg tacgggcccg tcatggggac catctattcc acgatcggcc
      661 tcaccatcgg ctcctggctg gccttcgccc tttcccgatg gctcggcctg cccttcgtgg
      721 aaaaggtggt ggaccggaag atcctccaga aatacgatcg gttcatggaa cgccaggggg
      781 cactcgtcac cttcgccatg tttttgatcc ctggttttcc gaaagacgcc ctgtgttata
      841 tcatggggct gagccatatg aggacaagcc tctttctgct cgtatcgacc gccgggaggc
      901 tcttcggaac cgtcctcctc tcggtcgggg gcagttgcat acgtaacgac cagacaaaga
      961 ccctgtacgc catccttgcc tttaccgccg tcgtccttgt cttcgcttat ctttaccgcg
     1021 ataagctctt gaagctcttg ggaaaaaaac aaccgcccct ggatagcggt aaggtaggtc
     1081 ccgacaccct tccagacaaa tcaccggaca aatctatgga caaatgaatc gcctttcttt
     1141 atgattagtc gcacgtatgc ggaatttttt gcacttttga ccataaccct caatcttgca
     1201 aaattttttc gggagaacct ggaaaatgac gaataaaaaa atttattttg aggaagtacc
     1261 ggtagaaaaa ctgcgatggc ggtgcaaccc cgaatcactg aagttccaga cgacggaaga
     1321 gtgcgaatac aaagagggga tcatcggtca ggaccgggcc gtcagggctt tgaagctcgg
     1381 actgaccatc gaaagcccgg ggtacaacat ctatgtctcc ggaaggacgg ggacgggcag
     1441 aacctcgacc atcatgagga tactgaacaa actcgactcc ggcaaaggga taccgaagga
     1501 catctgctat gtgaacaact tcaagaaccc cgacatgccc aaggtaattt ttcttcctgc
     1561 gggcatgggg cagaaattcg cccgtgacat ggacgacctc atcgaccatc ttatcaaaca
     1621 gatccccctg atcttcgaaa gcgacgtctt caagaaggcc tccgaagaga tcatagaaag
     1681 ctaccggtcg aagcaaaaag agatgatgaa ggaattcaat gaccgcatcc tgaaagacgg
     1741 gttccagctc gtggcctttc agatcgggcc tttcacccgg caggacatcg tccctgtcta
     1801 tgagggtaag ccggtgcctt tcgagcagct cgaggccctg gcggaacagg gcaacttcag
     1861 caaagaggaa ctggaaagga tcaagaagaa actcctcgac taccggatag agctcgattc
     1921 gttgatgagg gaaatgaggg agaacgaaaa gaagatccgc aaagagatcg acggcctgga
     1981 atacagaagc ggcctgccag tggtgagcga gctgatttcc gacatccggg tcaaatacag
     2041 cgacgacaac gcaaaaataa acgaatacct cgacgaggta caggagcaca tcctgtcgaa
     2101 cctcaagatg ttcaaggaaa aagaggagga gcaacaaccg caccccccca tcccgctgcc
     2161 gttcgtcgcg gtgccgcaac cgcggttcac ggagttcaag gtaaatgtcc tggtcgacaa
     2221 ctcccagacg aaaaaaacac cggtgatcct ggaaacagac ccgacttaca agaatctctt
     2281 cggcaccatc gagcgcgagg ttgaccgtgc tggcttctgg aggacggact ttaccaagat
     2341 caaggcggga tcgctcctcc gggcgaacgg cggctacctg gttttcatgg ccctggacgc
     2401 cctgatcgaa ccgggcgtat ggtacacgct gaaaaggacc ctcaagaaca ggtcgctgaa
     2461 catgcaaccc ttcgaccctt acggcatcat gccaacggcg ctcaagccgg aagccattcc
     2521 cctggacgtc aaggtcgtga tggtcggcga tgactatatc taccgacttc tttacgtgtt
     2581 cgaggaggac ttcaaaaaga tattcaagac gaaatccgac ttcgacacgg aaatgcctaa
     2641 cgaggaaaag cacgtcgacg attatgtctg cttcatcaag aggatcatcg acgacgagaa
     2701 gctcctccag ttcgacaggg ccgctgtagc ctccatcatc gaacacggtg tcaggctgac
     2761 gggacggcag aagaaactct ccacgcgttt cagcgaaatc accgacctga tccgcgagtc
     2821 ccattactgg gcccggcagg agggaagcgc gctggtcacc gacaaacatg tagaaaaggc
     2881 ctacgaagag aaggtaagcc gcgtcaacct catcgaagac aagatacagg agatgatcga
     2941 ggacggcgtc atcatgatcg acacggaagg cgcggtcgtg ggccagataa acggtttatc
     3001 cgtctacgac ctcggcgacc accttttcgg aaaaccgtcg aggatcacgg cgaaaacgtc
     3061 gatagggcgg gcgggcgtca tcaatatcga gagggaggcc aaactctcgg ggaagaccca
     3121 cgacaagggg gtcctgatcc tggagggcta cttccgcggc aagtatgccc aggataaacc
     3181 gctcacgatg agtgccagcc tctgcttcga gcagtcatac tccggcgtcg acggcgacag
     3241 cgcctcttcc acggaggtct acgcgatcat gtccagcctg gcgaacctgc cgatccggca
     3301 ggatctcgcc gtcacggggt cggtgaacca gaagggtgaa atacagccga tcggcggcgt
     3361 aaactacaag gtcgaaggtt tttacgacgt ttgtcgagcc aaagggttga ccggcacaca
     3421 gggggtcctc attcccgccc tgaacgtccc ggagctgatg ctgcgcaagg acgtcgtcga
     3481 ggccgtgagg cagggaaggt tccacatcta cccggtcaga acgatcgacg aagggatcgc
     3541 gatcctgacg ggggtggagg caggcgagag ggatgagacg ggtcagtatc ccgaaaatac
     3601 cgtcaacagg atggtggacg aaaggttgaa aaacatcgct ctcagcctga aaaccttcgg
     3661 cgaggaagag aagaaaccgg ggcaaggaca ggcgcccgag ccgtcggcct gagattacgg
     3721 tcgccgatct atctgggcaa ctcgaaaaac ttgcaa
//
LOCUS       DAOTMX010000160         3755 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_19491_length_3755_cov_3.550000, whole genome shotgun sequence.
ACCESSION   DAOTMX010000160 DAOTMX010000000
VERSION     DAOTMX010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19491_length_3755_cov_3.550000"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..783
                     /gene="folE2"
                     /locus_tag="P5573_05490"
     CDS             16..783
                     /gene="folE2"
                     /locus_tag="P5573_05490"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HRT27547.1"
                     /translation="MVDVQSLTDHRNINIKKVGVKGVKYPIIVLDRANGFQHVNATVN
                     MYVNLPHHFKGTHMSRFVEVLNEYRGQINIKTFRTILEKIKEKLNAESAHMEIEFPYF
                     IEKTAPSSGAKSLMEYICRFAGENNGQKTDFMVGVTVPVTTVCPCSKEISSLGAHNQR
                     SIVKVNLRFRKFFWIEDVIKLVEDSASGEVYSLLKRADEKYVTEKAYRNPMFAEDVVR
                     SVAEKLNRMDNFPWYSVEAENLESIHNHSAYAYVEKE"
     gene            799..963
                     /locus_tag="P5573_05495"
     CDS             799..963
                     /locus_tag="P5573_05495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRT27548.1"
                     /translation="MPTYEYQCKECKNEFSVVLSVSEHDKGNVSCPKCKKKGVKQLVT
                     PFIAQTSRKS"
     gene            complement(1059..2753)
                     /locus_tag="P5573_05500"
     CDS             complement(1059..2753)
                     /locus_tag="P5573_05500"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HRT27549.1"
                     /translation="MSFRRMAASSRRYRHFSRYRRILSVMVKYGFGEALYRLNLLRFL
                     GLVRSFVARRRVEGLETMTRAERVRMALEELGPTFVKMGQILSLRPDLIPLEFIGELS
                     KLQDSVTPFPFEEAKKIVESELKNPLSDVFASFAESPLAAASIGQVHRARLRSGEEVV
                     VKVQRPGIRRTVETDIEILQYFARLAERYIEEMRDFRPGAIVEEFARTIRKEMDYTVE
                     ASHLERFARQFRGNDALFVPAVFREFSTGKVLTMEYVEGIGISEVARLEEAGFDRKLL
                     AARGVDLMLEQVFKHGFFHADPHPGNILVLPGNVISYLDFGMMGSVDRKMRGYFVEVI
                     EGFADRDASKMADSLLKIVEWDEEPDRRRLERDIGEYADLYLYRPIKEWWIKNLVEGM
                     LALLSRHRLRIPPDIFLMLKAIAEVEGLGRLLDPDFELLERAKPFVRRMHLERMNPKR
                     LFEGLSESGGEMADFLKETPGDLRDILKQWKQGRTRIGFEHRGLERFIFEMDRSSNRI
                     AFSLIVSSLIVGSSLIVTADVGPHLYGFPVLGVAGFSVAGIFGMWLVISIFRSGRL"
     gene            complement(2761..3075)
                     /locus_tag="P5573_05505"
     CDS             complement(2761..3075)
                     /locus_tag="P5573_05505"
                     /inference="COORDINATES: protein motif:HMM:NF017415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HRT27550.1"
                     /translation="MKDYIRKAMLIGIGLAATTREKVEESIAELVKKGELSEKEGKEL
                     VNELLEKSKKIKEEWDKRVERAVTDALSRLSIPNKKELDELKMRVEALERTLEKGQEN
                     EG"
     gene            complement(3244..>3755)
                     /locus_tag="P5573_05510"
     CDS             complement(3244..>3755)
                     /locus_tag="P5573_05510"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRT27551.1"
                     /translation="PSISLTGLFGVSSEDLSNLFHGAAKIWNYTGNVTVPIFTAGRIS
                     GQVQAAEAVRRQALYGYQQAVQTAFREVEDALVDQRKSREKFEYESRQIEALRTYCQL
                     AGMRYENGYSSYLEVLDANRNLFAAELSHIQTKGNLFQAVVNLYKAMGGGWIVEAEKV
                     EAKTAPSSR"
BASE COUNT          845 a         1110 c          976 g          824 t
ORIGIN      
        1 acttgaagtt tcaccatggt tgacgtccag agtctcacag accacaggaa catcaatata
       61 aagaaggtcg gagtcaaagg ggtcaagtac ccgataatcg tccttgaccg ggcaaacggc
      121 ttccagcatg ttaacgccac ggtcaacatg tacgtaaacc taccccatca cttcaagggc
      181 acccacatga gccgattcgt ggaggtcctc aacgaatacc ggggtcagat caacatcaag
      241 accttccgca cgatcttgga aaagataaag gagaaactca acgccgaatc ggcccacatg
      301 gagatcgagt ttccctattt catagaaaaa acggcgccgt cctcaggggc caaaagtcta
      361 atggagtaca tctgtcgttt tgcgggcgag aataacgggc aaaagaccga ctttatggta
      421 ggcgtgacgg tccccgtcac tacggtctgc ccctgctcga aggaaatcag cagcctcgga
      481 gcccacaacc agcgtagcat cgtgaaggtc aacctccggt tccggaagtt tttctggatc
      541 gaggacgtga tcaagcttgt ggaagactcg gccagcggtg aggtctactc tctcctcaag
      601 cgcgccgacg aaaagtacgt caccgagaaa gcctacagaa acccgatgtt cgccgaagat
      661 gtcgtcagga gtgtcgccga gaagctcaac cggatggata atttcccctg gtacagcgtc
      721 gaggcggaaa acctcgagag catccacaac cacagcgcct acgcgtacgt ggaaaaagaa
      781 tgagcggagg gaatcgagat gcccacttat gagtaccaat gcaaagagtg caagaacgag
      841 ttttctgtcg tgctgagcgt ctctgagcac gataagggga acgtgagctg ccccaagtgc
      901 aagaaaaagg gagtcaaaca gctcgtcact cccttcatag cccagacgag caggaaaagt
      961 tgaatttcgc acacctcgcc ctgcggctac cgtttcgtat tccaaggccc gggagaaaag
     1021 cggggtgttg tttgcctccg tcgttcctcg accatttctc acagccttcc cgaccggaat
     1081 atggagatga cgagccacat cccgaagatg cctgccacac tgaacccggc cacccccagc
     1141 actgggaagc cgtagagatg gggtcctacg tccgccgtga cgatgagcga agacccgacg
     1201 atcagagaag aaacgatgag cgagaaggcg attcggttcg atgaccggtc catctcgaat
     1261 atgaagcgtt cgagcccccg gtgttcgaat cctatcctgg tcctgccctg cttccactgc
     1321 ttcagtatgt cccggaggtc gccgggcgtc tctttgagaa aatctgccat ctcgccgccc
     1381 gattccgaca atccttcgaa gagtctcttg gggttcatcc gctccagatg catccttctc
     1441 acaaaaggct tcgcccgttc cagaagttca aaatcagggt cgagcaggcg gcccaaccct
     1501 tcgacttccg cgatcgcctt cagcatcagg aagatgtccg ggggtatcct caggcggtgt
     1561 cgcgagagga gcgccaacat cccctccacg aggttcttga tccaccactc cttgatcggt
     1621 ctgtacaggt agagatccgc atactcccct atgtcccttt ccaaacgccg ccggtccggt
     1681 tcttcatccc attcgacgat cttgaggagt gaatccgcca tcttagaggc atccctgtct
     1741 gcaaaacctt cgattacttc cacgaaatac ccgcgcatct tcctgtcgac cgaccccatc
     1801 atcccaaagt cgagatagga gattacgttc cccggcaata ccaaaatatt gccgggatgg
     1861 gggtcggcat ggaagaaacc gtgtttgaag acctgttcca gcatcaggtc cacgccccgg
     1921 gccgccagca atttcctgtc gaaaccggcc tcttcgagcc gagcgacttc ggaaatcccg
     1981 atcccctcga catattccat cgtgagaacc tttcccgtcg aaaactcacg gaacacggcg
     2041 gggacgaaca acgcgtcgtt accccggaac tggcgggcga aacgctccag gtgggaagcc
     2101 tcaacggtgt aatccatctc tttgcgtatc gtccgggcaa actcttcgac gatcgctccg
     2161 ggcctgaaat ccctcatttc ttcgatgtac ctctccgcaa gccgggcgaa gtactggagg
     2221 atttctatgt ctgtctccac ggtccggcgg attccgggcc gttgcacctt gacgaccact
     2281 tcttcccccg acctcaggcg ggcacggtgg acctgaccga tggacgcggc ggctagcgga
     2341 ctttctgcga aggacgcgaa gacatcgctg agagggtttt tcaattctga ctcgacgatc
     2401 ttcttcgctt cctcgaaagg gaaaggcgtc acgctgtcct ggagtttcga cagctcaccg
     2461 atgaactcga gggggatcag gtccggcctc agtgagagga tctggcccat cttcacgaag
     2521 gtgggcccca attcttcgag ggccatcctc acccgttcgg ccctcgtcat agtctcaagg
     2581 ccctcgaccc gcctccttgc gacaaaagac ctcacaagac cgagaaaccg cagcagattg
     2641 agacggtaga gggcttcgcc gaaaccgtac ttgaccatca cggagaggat acggcggtac
     2701 ctgctaaaat gacgataacg gcgggaggac gccgccatcc tgcgaaatga catgactgaa
     2761 tcagccctcg ttttcttgtc ccttctccag cgttctttcc aacgcctcga ccctcatctt
     2821 cagttcgtcc agctccttct tgttagggat gctgaggcga ctgagcgcat cagtgaccgc
     2881 cctctccacc ctcttgtccc actcttcctt gatcttcttg gacttttcga ggagttcgtt
     2941 gacaagctct tttccttcct tttccgaaag ctcgcctttc ttgaccagct cggcgatgga
     3001 ttcttcgacc ttttctcgtg tcgttgccgc aaggccgatc ccgatcaaca tagcctttct
     3061 tatgtaatcc ttcatataag tctccctcct ttctatgtct tatatttagc tttctgcctg
     3121 tattgtatgg cattgtcaat atgaatctaa gggtgcgaat tgcgggagag caaatggtca
     3181 cgtgtgtaga taaacgtgaa aggacggctc gacgtcgcta ttctgcgcct tttgacgaca
     3241 ggatcagcga gaggaggggg cggtcttcgc ctcgactttc tctgcctcca cgatccagcc
     3301 gccgcccatc gccttgtaaa gattgacgac ggcctggaaa aggtttccct tggtttggat
     3361 gtgacttagt tcggcggcga agaggttccg gttggcgtca agcacctcga gatagctgct
     3421 gtacccgttt tcataccgca tacccgcgag ttgacagtag gtccggagcg cctcgatctg
     3481 gcgggactca tactcgaatt tttcgcgcga cttgcgctga tccacgaggg cgtcctccac
     3541 ctcacggaag gcggtctgga cggcctgctg gtaaccgtaa agggcctgcc ttcggaccgc
     3601 ttcggcagcc tgaacttgcc cgctgatccg gccggcggtg aagatcggca ccgttacgtt
     3661 gccggtataa ttccatatct tggccgcgcc gtggaatagg ttcgaaaggt cctcgctgct
     3721 cacaccgaac agacctgtca aagagatact gggga
//
LOCUS       DAOTMX010000161         3746 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_19539_length_3746_cov_4.486860, whole genome shotgun sequence.
ACCESSION   DAOTMX010000161 DAOTMX010000000
VERSION     DAOTMX010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3746
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19539_length_3746_cov_4.486860"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="P5573_05515"
     CDS             <1..248
                     /locus_tag="P5573_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27552.1"
                     /translation="LHPRQRYAPLKGTDKESLYLEGLDRSLYMNTFDFWWLHELKSDR
                     NITITTIVLTMLILMFIASALGIRAALRESPVRQVRD"
     gene            308..1717
                     /locus_tag="P5573_05520"
     CDS             308..1717
                     /locus_tag="P5573_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27553.1"
                     /translation="MMASIFTACIFAPGIMTNDSLGQYGEALRGTFGDWHPPVMSIVL
                     HFFLFFKGGLTWLTGLQSISGILGIFVFSHEILRLLANEKNKDANLILAACTLTVLFL
                     LPLTPTIFYFNTFIKDTWTAIVFLWVGAFSLRLFRQRQDMDKRSLQAGVFLLCLLMAF
                     SSLPRHNSLIVLIPMGLCLSLIVSGTKKKQASLFFLLPLLTWLLINIAMYSLFSVRHY
                     HHSNIVKALDLAGLCYINPDACRKLTYTRSSIDLDLLAKGYRFGNVHPLIWSEPPIAT
                     PGFTSAKPNPELDSEYLYALRNYPFDILRLKIESFMRLFDPRHTVNWFYFEIVPNPYG
                     LRMNSRFSSLRNVLKSMLEKVGRSLPGRCLAAAHLPWLLVNVLGVFCFLFAFRQRQDR
                     NSLFFVFLLLQPLSYYLSYLPATIYLDFRFMFPATLLMQVASGSFLCHVFLKAYGKKA
                     GMKRSGLRPPLPGAVKPFP"
     gene            complement(1721..3127)
                     /locus_tag="P5573_05525"
     CDS             complement(1721..3127)
                     /locus_tag="P5573_05525"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyltransferase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HRT27554.1"
                     /translation="MKLSILIPVYNEQYYIARTLDRVLRVDLPEDLEREIIVVDDGST
                     DGTREILETYQNKHGDVMRYVPQDRNYGKGAAIRKAIALATGDFCIFQDADLEYDPRD
                     YGLVLKPLLEGVADAVYGSRFLYRERRRVLYFRHSLGNRLLTFLSNLFTDLYLTDMET
                     CYKAFRTELLKTIPIRSDDFGLEPEITAKIAKRGFRVYEVPINYDGRTYAEGKKITWR
                     DGLLALYTICKFWLIDDCYQERKGQEILESFAHAHRFNKWLAALIKPYLGDSVLEIGA
                     GIGNLTKEFLPRKRYVASDIDPFYLNVLNNFALRRPGLEVIRVDAAVSGDFTDFRGAF
                     DTVLCINVLEHIADPERALRNFGTALSPDGRLLLLVPQGPFLYSVVDQAVGHVRRYTR
                     DELRKELKNAGFKIERLMDFNRVGVPGWLFNAKLLKRNSIPRFQLKLYDSLVWLWRRI
                     DRFLPWSGLSLIVVARKA"
     gene            complement(3188..>3746)
                     /locus_tag="P5573_05530"
     CDS             complement(3188..>3746)
                     /locus_tag="P5573_05530"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRT27555.1"
                     /translation="LFICGALKEIGGRRHIAIDLGPIRKEGSNELDRFGLGIYNLERC
                     GYSHLLEIWESPSELALPELLRRGRRIQFAFIDGWHSFDHTLVDFFYVNKMLDVGGVV
                     AFHDAVHPNVRKVIRHVMTYPAYRLYGASEIEKPVFSTTRKGPEKAIAYLLFLIRRFT
                     PRRPNCVALMKIEEDKRPWQWFVNF"
BASE COUNT          890 a          993 c          877 g          986 t
ORIGIN      
        1 ttctacaccc ccgccagcga tacgcgccgc tgaagggaac cgataaagag tctctctatc
       61 tggaggggct cgatcgttct ctctatatga acaccttcga tttttggtgg ctccacgaat
      121 tgaagagcga ccgaaatatt accattacta caatagtatt aactatgctt atattaatgt
      181 ttattgcctc tgctttgggc atccgggcgg cattacgcga aagcccggtt aggcaggttc
      241 gagattgata gtctgttctc gtcgttttgc cgatcgttgg tactggtgga catcgttatt
      301 ttttcttatg atggcgagta tctttacggc ctgcatcttt gcgccgggaa tcatgacgaa
      361 cgactccctc ggacagtatg gcgaagccct tcggggcaca tttggagact ggcacccccc
      421 cgtcatgagt atcgttcttc atttctttct atttttcaaa ggaggattga cctggctgac
      481 aggacttcag agcatcagcg gcattctcgg catctttgtt ttcagccatg aaatcttgcg
      541 tctcctcgcc aatgaaaaaa ataaagatgc aaatttgata ttggcagctt gtacactgac
      601 tgttttgttt ctccttccct tgacaccgac catcttttat ttcaatactt ttatcaagga
      661 cacgtggacg gccattgtct ttctttgggt cggcgccttt tccctgcgac tgtttcgcca
      721 aagacaggat atggataaac gatcgctaca ggcaggtgtg tttcttctct gcctcctgat
      781 ggccttttct tccttgccgc gtcataattc cctaatcgtt cttatcccta tgggattatg
      841 cctctctctg atcgtctcgg gaacaaaaaa aaagcaggcc agcctcttct tcctgttgcc
      901 actgttgacg tggcttttga taaacattgc catgtattct ctcttttccg taagacatta
      961 ccatcactca aatatcgtca aagccctcga tctcgcaggt ctttgttaca tcaaccccga
     1021 cgcatgtagg aagctgacct acacaaggag cagcatcgac cttgaccttc ttgccaaagg
     1081 ctaccgtttc ggtaatgtac accccctcat ctggagtgag cctcccatcg ctacacctgg
     1141 atttacaagt gccaaaccta atcctgaact tgatagcgag tatctttacg ccctgcgaaa
     1201 ttatcctttc gacatcctaa gattgaaaat cgaatccttc atgcgccttt tcgacccgcg
     1261 gcatacggta aactggttct attttgaaat tgtaccaaat ccctatggtt tacggatgaa
     1321 ctcccgcttc tcgtcgctcc gtaatgtctt gaaatcaatg ttggaaaaag tggggaggtc
     1381 actcccaggg agatgtttgg cggccgcgca cctgccgtgg ttacttgtca atgtccttgg
     1441 ggttttctgt ttcttgttcg cgtttcgcca acgacaagat cgtaacagcc ttttctttgt
     1501 ttttttgctc ctccaaccac tatcctatta cctgagctac ttgccggcga cgatttactt
     1561 ggatttccgt ttcatgtttc ccgcgacttt attgatgcaa gttgcgtcgg gatcgtttct
     1621 ttgtcatgtt tttttaaaag cctacggcaa aaaggcaggc atgaaacgct caggacttcg
     1681 accccctttg ccgggagctg tgaaaccttt tccttgatcc ttatgccttc cgggccacga
     1741 cgatcaagga cagacccgac cagggcagga atcgatctat cctcctccac agccatacca
     1801 gtgaatcgta gagcttcagt tgaaaccgag gaatgctgtt gcgtttgagg agtttggcat
     1861 taaatagcca accgggcaca ccgacacggt taaaatccat cagcctttct atcttaaaac
     1921 ccgcgttctt cagctctttc cggagctcat cgcgagtgta ccggcgtacg tgtcccaccg
     1981 cctgatcaac aacggaatag aggaaagggc cttgcggcac aagtagaagc aagcggccgt
     2041 caggggaaag ggcggtgccg aaattcctca aggccctttc aggatcggca atatgctcca
     2101 agacattgat acagaggacc gtatcaaagg cgcctctaaa atcggtaaaa tcccccgaca
     2161 ccgcggcatc gacccgaatc acctccaacc cgggtcggcg taaggcaaaa ttattcaaga
     2221 catttaagta aaaagggtcg atgtcgctgg ccacatatct ctttcgggga agaaactctt
     2281 tcgtgagatt accgataccg gccccgatct ccaggacgga atccccaaga tatggtttaa
     2341 ttaaggccgc cagccacttg ttgaatcgat gggcatgggc aaacgactcc aggatctcct
     2401 gccctttcct ttcttgatag cagtcgtcga tgagccagaa cttacagatg gtgtaaaggg
     2461 cgaggagtcc gtcccgccag gtgatcttct tcccttccgc ataggttctg ccatcgtaat
     2521 ttataggcac ctcgtagacg cgaaaacctc tcttggctat cttggcggtg atctccggct
     2581 ccagaccgaa atcgtcactg cggatgggga tcgtcttgag caattccgtg cggaaggcct
     2641 tgtagcacgt ctccatatcg gtaagataga ggtcagtgaa cagattcgac aaaaaggtaa
     2701 ggaggcgatt gcctaggctg tggcgaaaat aaagaacgcg ccgccgctcc cgatagagaa
     2761 atcgtgaccc gtagaccgca tcggccactc cttccaacag cggctttaat acgagtccat
     2821 agtcgcgggg atcgtactcc aggtcggcat cttgaaaaat gcagaaatcg cccgtggcca
     2881 gggcgatggc ctttctgatg gccgcgccct tcccataatt gcggtcctgg gggacatagc
     2941 gcatcacatc cccgtgctta ttctgatacg tttctaaaat ctccctggtg ccgtccgtgg
     3001 atccatcgtc cacaacgatg atttctctct ccagatcttc aggaaggtcc acgcgcaaaa
     3061 cgcgatcgag ggtgcgggcg atgtaatact gttcattgta aacggggatt agaatgctaa
     3121 gtttcattag gtaccccttc tgtcgaataa tatcaataga ttacatcatt catgccttat
     3181 gaagacatta aaaattcaca aaccactgcc acgggcgctt gtcctcttct atcttcatta
     3241 gggctacaca gttcggacgc cggggcgtaa atcgtcgaat caaaaaaagg agataagcga
     3301 tggccttttc cggaccttta cgggtcgtgc tgaagaccgg tttctcaatc tcggacgcac
     3361 cgtagagacg gtaagcggga taggtcatca catggcggat gacttttcgc acattggggt
     3421 gcacggcgtc atgaaaggcc accaccccgc ccacgtccag catcttgttg acatagaaga
     3481 aatcgacgag ggtatgatcg aaggagtgcc aaccatcgat aaaggcaaat tgaatgcgac
     3541 gaccccggcg aagcaattcg ggcaaggcaa gttcagacgg cgattcccat atttccagga
     3601 ggtgactgta tccacagcgc tcgagattgt aaattcccag accgaatcga tccaattcat
     3661 tgctgccctc tttacggatc ggtcccagat cgatggcaat gtgccttcgc cccccgatct
     3721 ctttcagggc cccacagatg aaaagg
//
LOCUS       DAOTMX010000162         3723 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_19698_length_3723_cov_4.133588, whole genome shotgun sequence.
ACCESSION   DAOTMX010000162 DAOTMX010000000
VERSION     DAOTMX010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3723
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19698_length_3723_cov_4.133588"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="P5573_05535"
     CDS             <1..562
                     /locus_tag="P5573_05535"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRT27556.1"
                     /translation="VVYNAGLLKLDFLDPVSSFFENFNPSGEKAPVGAAGVKKPLSRT
                     DGGQHQQALKPDVPSYEPANNPDIVVKGAHEAVSVPPGKEGDKSPTKDEVFYSIQVRA
                     VQRPEEAADFVKTLSARGMEAFYQKVALKDSGLWYRIFIGRFADEGAARRYYSKNNIG
                     ESFPGCFIRRIPVDKSIEKKDKGERR"
     gene            581..1969
                     /locus_tag="P5573_05540"
     CDS             581..1969
                     /locus_tag="P5573_05540"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRT27557.1"
                     /translation="MNHVNKNVLFLLTFVSLTTLLAGWFISHVNVNTAFLGLLSVILV
                     ILCFLNTEVGLYILIVAMLLGPQFIAGGTHTEFVEVRGRALTLRLDDFLLLAVGLGWF
                     TRAAIDKELGLFLKTPLNRPIAYYFIACVVSTMVGAMMGRVKPAPGFFFVLKYFEYFV
                     IYFMAVNHLREKKQVERFILTILIVCFVVCLISMTQIPSGARVSAPFEGEGGEPNTLG
                     GYLDFILALVLGLLLTNGIPRYRLFLLILVPTILIVLMATLSRSSWLSLLPLILTLIV
                     FSKKKMVLIISVLFVVLLSPFILPKSVVDRALYTFTQPPEEGQLEIGQVKIDTSTSSR
                     IFSWKEALSKAWVKHPILGYGVTGYRFLDAQFPRVLVETGLLGFFTFLFLLRSIFKVS
                     HSVYRQTEDPFFSGITLGYLAGFFALLTHAIGANTFIIVRIMEPFWFLTAIVVMIPRI
                     EGSSSPPSADTV"
     gene            complement(1943..3124)
                     /locus_tag="P5573_05545"
     CDS             complement(1943..3124)
                     /locus_tag="P5573_05545"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRT27558.1"
                     /translation="MERIKVIHVITRFDKGGSAENTFLTVIGLDKHKYDVLLVKGLSV
                     ESAMGEEERKAVDRSLARAEQAGVRITTVPDLVRDIRPVKDWRAFWELVKIFREERPH
                     IVHTHTSKAGFIGRWAAWLSKVPLIVHTPHGHVFWGYFNRPTSLLYVLLERWTARITD
                     RIVVLTEQEKKDHLLFRIAPEYKFVVIHSGVDLDRFRSVCRDPARIKEDLGIPSRAVV
                     VGTVGRLTPIKGHRFLIEAARGILAEWPDTVFLFLGDGELAEALRSLATRLGVSGSVR
                     FLGWRPDVAEILPAVDMFVFPSLNEGMGRAVVEAMALGKPVVASRVGGIIDIVRDGEN
                     GLLVSPADADELAATIRSLLSDPATRERLGEEGKRRVLLYDAERMVQRIDGLYSIGGR
                     R"
     gene            complement(3132..>3723)
                     /locus_tag="P5573_05550"
     CDS             complement(3132..>3723)
                     /locus_tag="P5573_05550"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HRT27559.1"
                     /translation="SWNEIFAREQREGLLRRRTDGNPDPGRELLSTRLREFQGSRLNR
                     MLYADFKETLPGDMLQKVDAMSMLNSLEVRVPLLDHHVCELAFSFHDDVKMKRGRGKY
                     ILIDTFKHLLPPSLHRRPKWGFEIPIGKWMKGELKYLLDEYLSRDRIERQGIFHYGMI
                     HRMVQDLMNYRSDTSWQLWNLVVFQNWYERHIGRSG"
BASE COUNT          790 a         1068 c          968 g          897 t
ORIGIN      
        1 agtcgtttac aatgccgggt tactaaagct cgacttcctg gatccggttt cgtccttttt
       61 cgaaaacttc aatccctccg gagaaaaggc gccggtcggc gcggccggcg tcaagaagcc
      121 cctttcccgg acggacggtg gtcagcacca gcaggcgctc aaacctgatg taccctctta
      181 tgaacccgcg aataatcccg acattgtcgt taaaggtgcc catgaagccg tatccgttcc
      241 gccgggaaag gaaggagaca aaagtccgac aaaggatgag gtgttttatt ccatccaggt
      301 ccgggccgtc cagcgcccgg aggaggcggc tgattttgtg aagaccctct cagcaagagg
      361 aatggaagcc ttctaccaga aggttgcctt aaaagattcg gggttgtggt accggatttt
      421 tatcggccgt tttgccgatg aaggagccgc gcgccgttat tacagcaaaa acaacatcgg
      481 cgagtctttt cccggatgct tcattcgtag gataccggtc gataagtcaa tagaaaagaa
      541 agacaaaggg gaacgacgtt agtcttgtcc atgcaaaaac atgaaccatg tcaataagaa
      601 tgttctattc cttcttacct tcgtaagttt aaccaccctc ctcgcgggtt ggttcatctc
      661 ccatgtaaac gtaaataccg cctttttagg cctactcagc gtcattttag tcatcctgtg
      721 ctttttgaac acggaggtcg gactttacat tctcatagtc gccatgctcc tgggtcctca
      781 gtttatcgcc ggcggcacac acactgagtt tgtggaggtt cgtgggcgcg ccctgacatt
      841 gaggctcgac gacttcctgc tccttgcggt cggattgggg tggttcaccc gggcggctat
      901 cgacaaggaa ctcggccttt ttctcaaaac tcccctgaac cggccgattg cctactattt
      961 tatcgcctgc gtcgtgtcga cgatggtcgg cgcgatgatg ggcagggtca aaccggcgcc
     1021 gggctttttt ttcgtcttga aatactttga gtactttgtc atctacttta tggccgtcaa
     1081 tcacctccgg gagaaaaagc aggtagagcg ttttatcctg actatcctga ttgtctgctt
     1141 tgtcgtctgt ctcatttcaa tgacgcagat cccctcggga gcgagggttt ccgcaccgtt
     1201 tgagggtgaa gggggcgaac cgaacaccct cggaggatac ctcgacttca tcctcgcgct
     1261 cgttctcggc ctgctgttga caaacggtat acccaggtac agactttttc tccttatcct
     1321 tgtgccgacg atcctcatcg tactcatggc taccctgtcc cgcagctcct ggctctcact
     1381 gttgcctctg atattgacac tcatcgtctt cagtaagaag aaaatggtgc tcatcatatc
     1441 cgtgcttttc gtcgttttac tgtcgccgtt catcctgccc aagtccgttg tcgatagggc
     1501 actctacact ttcacccagc cgcccgaaga agggcaatta gagataggtc aagtgaaaat
     1561 cgatacctcc acttcatcac gtattttctc ctggaaagag gctctctcga aggcctgggt
     1621 taaacatccc atccttggtt acggcgtcac cggttaccgt tttttagacg cccagtttcc
     1681 ccgagtcctt gtcgagacgg gattgctggg gttttttacg ttcttgttct tgctccgttc
     1741 tatctttaag gtctctcatt ccgtctacag acaaacagaa gacccttttt tcagcggcat
     1801 caccctcggc tatcttgccg gatttttcgc cctcctgacc cacgcaatcg gcgccaacac
     1861 cttcatcatc gtgcggatca tggagccttt ttggtttctg accgcgatcg tcgtcatgat
     1921 cccccggatc gaagggtcct catcaccgcc ttccgccgat actgtatagg ccatcgattc
     1981 tctggaccat tctttcagcg tcatatagaa gcacccgtct ttttccttcc tcccccagcc
     2041 gttctctcgt ggcgggatca gagagcagcg accttatcgt cgcggccaac tcgtcagcat
     2101 cagcagggga aaccagcagt ccgttttcac cgtcccggac gatatcgatg atcccaccaa
     2161 cccggctcgc gacaaccggt ttgccgagag ccatggcctc gaccacggcc ctccccatgc
     2221 cctcgttgag agaaggaaag acaaacatat cgacggcagg gaggatttcg gctacgtccg
     2281 ggcgccagcc gaggaaccgg acactcccgg atacgccgag ccgggtcgca agtgaacgca
     2341 gagcctctgc aagttcgccg tcgccgagaa aaagaaagac ggtatcgggc cattcggcga
     2401 gaataccccg ggccgcctct attaagaagc ggtgcccttt tatgggggtc aatctcccga
     2461 cggttccgac aacgacggcg cgggagggaa taccaaggtc ctccttgatt cgggcaggat
     2521 cccggcagac gctgcgaaat ctgtcaaggt cgacaccgct gtggatgacc acgaatttat
     2581 attcgggagc gatgcgaaaa agaaggtgat cttttttttc ctgctccgtc aggacaacga
     2641 tcctgtccgt gatgcgggcc gtccatcgct cgagcagcac atagagcagg gaggtcggcc
     2701 ggttgaaata tccccagaag acatggccgt ggggggtatg gacgatcagg gggaccttcg
     2761 acagccaggc ggcccagcgt ccgatgaagc cggccttgga ggtgtgagta tggacgatat
     2821 gcggccgctc ttcgcggaaa atcttcacaa gctcccaaaa agcgcgccaa tcctttacgg
     2881 gacggatatc cctgacaagg tccgggacgg ttgtgattct gacgccggcc tgttccgccc
     2941 gggcaagact gcgatcgacg gccttcctct cttcctcccc catcgcggat tccaccgata
     3001 accccttgac gagaagaacg tcatatttat gcttgtccaa tcctataaca gtcagaaagg
     3061 tgttttcagc cgaacccccc ttgtcgaacc gcgtgatgac gtggatgact ttgatgcgtt
     3121 ccatcgacgg atcacccgct cctgccgata tgccgttcgt accagttctg gaacacgacc
     3181 aggttccaga gttgccagga ggtatcagac cggtagttca ttagatcctg caccatccga
     3241 tggatcatgc cgtaatgaaa tataccctga cgctctatcc ggtcacggga aagatattcg
     3301 tcaagcaggt atttcaattc acccttcatc cacttgccga tcgggatctc aaagccccac
     3361 ttcggtcgcc ggtggagtga agggggcaac aggtgcttga acgtgtcgat gaggatgtac
     3421 ttgccgcggc ctcttttcat cttgacgtcg tcgtggaagg aaaaggccag ctcgcagaca
     3481 tgatggtcca gcaggggaac cctcacctcg agagagttca gcatgctcat ggcgtccact
     3541 ttttgcagca tgtcccccgg gagggtctcc ttgaagtccg cgtagagcat ccggttgagc
     3601 cgggagcctt gaaattcacg gagccgcgtg ctgaggagtt cccggccggg atcgggattg
     3661 ccgtccgtcc ggcgtcgcaa aagaccttcc cgctgctctc ttgcaaaaat ctcgttccag
     3721 gac
//
LOCUS       DAOTMX010000163         3712 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_19763_length_3712_cov_2.829368, whole genome shotgun sequence.
ACCESSION   DAOTMX010000163 DAOTMX010000000
VERSION     DAOTMX010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19763_length_3712_cov_2.829368"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="P5573_05555"
     CDS             <1..817
                     /locus_tag="P5573_05555"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRT27560.1"
                     /translation="IGLVQVGLQAMADRYTYVPLTGIFVMVAWGIPELLQKVAHREKV
                     MAAAGVAVVAALSTLTYIQTAYWKDDTTLFRHAVEVTTDNYWAHYNLGLTLAKRGELD
                     RALDHFKETVRVKPDEPDAYNNIGIILAKKGQLKEAVDNFSEALRKDPANEVARKNLA
                     VALSQMAKKGEKLPAFEHPPSGPGDAESYYRLAVENAQKVNLKEALSCFSEVLRIRPD
                     HFRAHNDMGVILARTGRLDEAITHFREALRAKPDFEEARYNLKVATAQKASSR"
     gene            complement(883..1674)
                     /locus_tag="P5573_05560"
     CDS             complement(883..1674)
                     /locus_tag="P5573_05560"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRT27561.1"
                     /translation="MDLKLKDRVALVAGGSQGLGRAAALQLAKEGAKIAICSRGGPNL
                     SEAAELIKKETGQEVFAVPADLTDAAQARGFVRKSVEHYGTVDILVNNAGGPPSVSFE
                     EIDDDLWHLGFRLNLMSTIVMTREAVPTMKAKRWGRIINMTSIAVKQPIDGLILSNTV
                     RSGVIGFAKSLSNELAPFNITVNSVCPGYTLTDRVRNLSKVLAEKQKTTPEAVIKKWE
                     SEIPMGRLGRAEEFATLVAYLASEGAGYITGAAIQIDGGWYKGVM"
     gene            complement(1753..2634)
                     /locus_tag="P5573_05565"
     CDS             complement(1753..2634)
                     /locus_tag="P5573_05565"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRT27562.1"
                     /translation="MKGQIVFRAVRSFFRLIPFPVIWRVGDGVGLLLYRLLKKRSAIG
                     LANLSRAFPDMPERERVEILKTFWRNFCKDMLEAIRYYAYPAAVKGKRVTLVGRERLD
                     ALLAQDTGIVVLSAHLGNFPLLCMKLGMEGYPIAVVYGEMHNRFFNPVLPHMMRSTGI
                     EPIFDRPRHACVGKSISWLRNKGMLFIQVDQNPLVEAGVPVDFFGFKVPTSRGPVSLA
                     MRTAAKIVPVFIVRDKNNHHRVIVEEPVALRLEGNGNHHMRENLEALSKVTEDYIRRY
                     PTLWWWVHQRFKMAVKE"
     gene            complement(2631..>3712)
                     /locus_tag="P5573_05570"
     CDS             complement(2631..>3712)
                     /locus_tag="P5573_05570"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fatty acid--CoA ligase family protein"
                     /protein_id="HRT27563.1"
                     /translation="PPDSDLRLERPPEDEAACLYTSGSTGRPKLAVLTFENLSCFPRA
                     LRDIYATTPEEVCGMLLPMSHVSGPIAIQESVENGTTMVIFDLMTERRGILRSFQENK
                     VSLVWGVMPIYQLLIHEAKLREFDTRGLRLLAVMGMETPVSFLRELSKVFPHTAVVQG
                     YGLTETTGAVIGTPPAEFRRKMESIGRPASFMEVRVVDPEGRPLPPKQYGEIVMRGPA
                     VMKGYYRDEAATRERIRDGWLYTGDLGYLDEEGYLYLLGRTDDMIITGGLNVFPGEVE
                     DVIRRHPKVKDVAVVGIPDPRRGAIVKAFVVPSSETTKEEILKHCRANLPGFKCPRII
                     ELREELPMTSSGKISRSALRRAGRS"
BASE COUNT          735 a         1188 c         1028 g          761 t
ORIGIN      
        1 catcggactc gtccaggtcg gactgcaggc gatggccgac cgctacacgt acgtcccctt
       61 gaccggaata ttcgtgatgg ttgcctgggg gataccggag ttgctgcaaa aagtagctca
      121 ccgggagaag gtcatggcgg ctgcgggggt ggcggttgtt gcggcgttga gtacactgac
      181 ctacatccag acggcgtact ggaaagacga cacgaccctc ttccgacatg ccgtggaggt
      241 cacgacagac aactactggg cgcactacaa cctgggtctg accctcgcca aacgggggga
      301 actcgaccgc gccttagacc acttcaagga aaccgttcgg gtaaagccgg acgaacccga
      361 cgcctacaac aacatcggga tcatcctcgc gaaaaaggga caactgaaag aagccgtcga
      421 taatttctcc gaggccttga ggaaggaccc tgccaatgaa gtcgcccgga agaacctcgc
      481 cgtcgccctt tcccaaatgg caaagaaagg cgagaaactc cccgccttcg aacacccccc
      541 gtccggtccc ggagatgctg aatcctacta ccggttggcg gtcgagaatg cgcagaaagt
      601 taatctcaaa gaggcccttt cctgtttttc cgaggtcctg aggatcaggc ccgaccactt
      661 tcgggcccat aacgacatgg gcgtcatcct ggcgcgaacg gggagactgg acgaggccat
      721 cacccacttc agggaagccc tccgggccaa acccgatttc gaagaagcca ggtataactt
      781 gaaggtcgcc acggcccaaa aggcctcatc acgctgagat gtgacgaagt gggattcccg
      841 tctttctgat gcacaacgcc cgatgtccgg cgcctcacct ttttacataa cccccttata
      901 ccatccgccg tcgatctgga tggcggcccc tgttatgtag cccgctccct ccgaggccag
      961 gtaggcaacg agggtggcga attcctccgc cctccccagg cggcccatgg ggatttccga
     1021 ctcccacttc ttgatcaccg cctcgggcgt cgtcttctgt ttttccgcca gcaccttcga
     1081 gaggttccgg acgcggtccg tgagggtata cccgggacag acactgttga cggtgatgtt
     1141 gaagggcgcc agttcgttgg acagggactt ggcgaaaccg atcacccccg aacgaaccgt
     1201 attggaaagg atcaggccgt cgatcggctg cttcacggcg atagaggtca tattgattat
     1261 gcgcccccag cgcttcgcct tcatggtggg gacggcctcc cgggtcataa cgatcgtgct
     1321 catcaggttg aggcggaaac ccaggtgcca caggtcgtcg tcgatctctt cgaagctgac
     1381 cgacgggggc ccaccggcgt tgttgaccag gatatcgacc gtcccgtaat gctcgactga
     1441 tttcctgacg aagcccctcg cctgggcggc gtccgtcagg tcggccggga cagcgaatac
     1501 ctcctgccct gtctctttct tgatgagctc cgcggcctcg gacaggttcg gaccgccacg
     1561 ggagcagatg gctatcttcg caccctcttt cgccagttgc agcgccgccg ccctaccgag
     1621 cccctgactt ccgccggcta cgagcgccac tcggtccttc aatttcaggt ccatggtccc
     1681 ctcctcgact cttttcgttc tccataccag atcgaggccg ccaccgtcaa cggccctgcc
     1741 cctccgcctt acttactcct tgacggccat cttaaacctc tgatggaccc accaccagag
     1801 ggtcgggtac ctccttatgt aatcctccgt gaccttgctc aaggcctcga ggttctctct
     1861 catatggtgg ttaccgttgc cctcaagccg caaggcgacg ggctcctcta cgattacccg
     1921 gtgatggttg tttttatccc gcacgatgaa aacggggacg atctttgcgg ccgtcctcat
     1981 cgccaggctg acaggcccac gggatgtcgg caccttgaac ccgaaaaagt cgaccgggac
     2041 gcccgcctct accagggggt tttggtcgac ctggatgaac aacatcccct tgttcctcaa
     2101 ccaggatatg gatttgccga cacaggcgtg acgtggcctg tcgaagatcg gctcgatgcc
     2161 cgtgcttctc atcatgtggg ggagaacggg attgaagaac cgattgtgca tttcgccgta
     2221 gacgaccgct atcgggtacc cctccatgcc cagcttcatg caaaggagcg gaaaattccc
     2281 gagatgggcg ctgagaacga cgatccccgt gtcttgggcc aggagggcgt cgagacgttc
     2341 cctgccgaca agggtgaccc gcttaccttt gaccgccgcc gggtaggcgt aatacctgat
     2401 tgcctccagc atgtccttgc agaagttccg ccagaacgtc ttcaatatct cgaccctctc
     2461 cctctcgggc atgtccggga atgcccgcga caggttggcg aggccgatgg cgcttctctt
     2521 cttcaagagg cgataaagga gaaggccgac cccgtcccca acccgccaaa tcacgggaaa
     2581 gggtatcagc ctaaaaaacg acctgacggc cctgaaaacg atttgaccct tcatgacctc
     2641 cctgcccggc gcaaggcact acgggatatc ttacctgacg acgtcatcgg cagttcctcc
     2701 cttaattcga ttatccgggg gcacttgaat cccggcaggt tggcccgaca atgtttcaga
     2761 atttcttctt tcgtggtttc ggaagaaggg acgacgaagg cctttacgat cgccccacgc
     2821 ctggggtcgg gtatcccgac cacggccacg tccttcacct tcggatgcct ccggatcacg
     2881 tcctccactt cgccgggaaa gacattcagt ccgcccgtga tgatcatatc gtccgtccgc
     2941 cccagcaggt agagataacc ttcttcgtcg aggtaaccga ggtctcccgt gtagagccaa
     3001 ccgtctctta tccgttccct tgtggccgcc tcgtctcggt agtaaccctt catgacggct
     3061 ggccccctca tgacgatctc tccgtactgc ttcggcggga gaggcctccc ctccgggtct
     3121 acgacccgga cctccatgaa gctcgcgggg cggccgatac tctccatctt ccggcgaaac
     3181 tccgcaggag gcgtccctat cactgccccg gtggtttccg tcaacccata accctggacg
     3241 acggccgtgt ggggaaagac ctttgatagc tcgcgtaaaa agctcaccgg ggtctccatc
     3301 cccatgacgg cgaggagtct caagccgcgg gtgtcgaact cccttagctt tgcctcatgg
     3361 atgagaagct ggtagatggg catcaccccc cagacgaggg agaccttgtt ttcctgaaag
     3421 gaccgcagga tgcccctcct ctccgtcatc aggtcgaaga tgaccatcgt cgttccgttt
     3481 tcgaccgatt cttggatcgc gatcggcccc gacacatggg acatcggcaa cagcatcccg
     3541 cacacctctt cgggggtggt cgcgtagatg tcacgcaatg cccgcgggaa gcaactcagg
     3601 ttctcgaagg taaggacggc gagctttggc cgtccggtgc ttcccgacgt gtagagacag
     3661 gccgcttcat cctcgggcgg gcgttccaac cgcaggtcgg agtccggcgg tt
//
LOCUS       DAOTMX010000164         3710 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_19773_length_3710_cov_2.874145, whole genome shotgun sequence.
ACCESSION   DAOTMX010000164 DAOTMX010000000
VERSION     DAOTMX010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19773_length_3710_cov_2.874145"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..560)
                     /locus_tag="P5573_05575"
     CDS             complement(84..560)
                     /locus_tag="P5573_05575"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRT27564.1"
                     /translation="MGRWINTAGRGFNIVASAAVILMMMLTCADVVLRLFRQPIPGTY
                     EIVGFLGTVVASFSLAYTSIEKGHIAVEILVDRLPERAQAFFDALSSLIGASLFGLLT
                     WQSSLYATDLKLSGEVSLTLEIPTYLFVYGVAAGCGMLTLVLFVELGGALRKATRR"
     gene            complement(608..1633)
                     /locus_tag="P5573_05580"
     CDS             complement(608..1633)
                     /locus_tag="P5573_05580"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRT27565.1"
                     /translation="MKKFLTVCFSVALLVLTCAVTQPVQAAQTKLTYSVFFPPTHGQA
                     KAADSWAKEIEKRTHGQVKITILTGGTLTPADQTYDGVAKGIADIGMSCFAYTRGRFP
                     VMETLDLPLGYPNGKVATRVANDFYEKFRPKELAGVKVLYIHAHGPGLLHTVRPVRSL
                     ADVKGMKIRSTGLSSKVVSALGAVPVAMPQGETYESLQKGVVEGTITPIETLKGWKQA
                     EVIKYTTDCKDIGYTTAMFVVMNLRKWNALSPDVKKVFEDVSKEWIDVHGKEWDRLDA
                     EGKEYSLSLGNKIIPLSKEEGARWKAAVKPIINEYIRTTDGKGLPGKAAVAEAESLIK
                     KYSNKYR"
     gene            complement(1800..2093)
                     /locus_tag="P5573_05585"
     CDS             complement(1800..2093)
                     /locus_tag="P5573_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27566.1"
                     /translation="MTVLVGGVAAVLLGLIGLFAWWKDLFVILKGSIPLILLLGGAFA
                     VYVGIDELKEKVQQERESEKEELIKTREELEKAKAEAEKMREELEKIREERGL"
     gene            2309..2683
                     /gene="hisI"
                     /locus_tag="P5573_05590"
     CDS             2309..2683
                     /gene="hisI"
                     /locus_tag="P5573_05590"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HRT27567.1"
                     /translation="MFEPDFDKGNGLIPAIVQDFDSKEVLMLAYVNRQAWLSTLETGK
                     ATYWSRSRNSLWVKGESSGNVQLIREILVDCDSDTLLFKVEQVGGAACHTGYQSCFYR
                     RISDGKEEIVGKKVFDPEDVYK"
     gene            2686..3552
                     /gene="hisG"
                     /locus_tag="P5573_05595"
     CDS             2686..3552
                     /gene="hisG"
                     /locus_tag="P5573_05595"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HRT27568.1"
                     /translation="MVRLGIPKGSLEESTVELFKRAGWRITYDARSYFPDIDDDEISC
                     ILVRAQEMSRYVEEGVLDVGLTGRDWVVENDSDVVVVQELVYSKVTARQARWVLVVRE
                     DSPIRSLEDMEGKRISTELVNFTKRYFRERNIDVHVEFSWGATEAKCVEGLVDAIVEV
                     TETGSTIRANNLRIVHELMKTNTQLIANKDSWADPVKKRKIEQVMVLLNGSLQAIGKV
                     GIKLNVARENLGRVMLLLPSLKAPTVSGLYNEDWFAVETIVEKNIVRDLVPLLQEAGA
                     EGIIEYPLNKVI"
BASE COUNT          867 a          942 c         1025 g          876 t
ORIGIN      
        1 ggccatcacg aagcccacgg gaatcctcat gaacatgagc gcaaccagga cgaagaatcc
       61 gatgatgccg actgccgtag gggtcatctt ctcgtcgcct ttcgcagagc gccgccgagt
      121 tcaacgaaca ggaccagggt gagcatgccg cagcccgccg ccacaccgta gacgaagaga
      181 taggtgggaa tctcaagggt caacgagacc tcgccactca gtttcaggtc cgtggcgtaa
      241 agactgctct gccaggtcag gagcccaaaa agggaagcac cgataagtga gctcaaggcg
      301 tcgaagaagg cctgagccct ctcaggaagc ctatctacga gtatttctac ggcaatatga
      361 cctttttcta tcgatgtgta ggccagggag aaggaagcga cgaccgttcc gaggaacccc
      421 acgatttcgt aagtgcccgg tatcggctgc cggaagagcc ggaggacgac gtcggcgcag
      481 gtaagcatca tcatgaggat gactgctgcc gacgcaacga tgttgaaccc gcggcccgct
      541 gtgttaatcc atctgcccac aggcagcata gtctacatcc cttccgattg tctttgctat
      601 ggactctcta cctatattta ttactatatt ttttgatcag gctttccgct tccgcgacgg
      661 ccgccttgcc tggaagaccc tttccgtcgg tggtcctgat gtattcattg atgatgggtt
      721 tgaccgcagc cttccacctc gccccttctt ctttggagag agggattatt ttattaccca
      781 aactcaggct gtattccttt ccttcggcat cgagccgatc ccattccttc ccgtgaacgt
      841 cgatccactc tttgctgacg tcctcgaata cctttttgac atccggggac agggcattcc
      901 acttccgtag attcatgacg acgaacatgg ccgtcgtgta tccgatgtcc ttgcagtccg
      961 tcgtgtattt tatcacttcc gcctgtttcc agcccttcag cgtttcaatg ggtgtgatcg
     1021 ttccttctac aacgcccttc tggagggatt cataggtttc tccctgcggc atggctacgg
     1081 gcacggcccc gagggcggag acgaccttgg aactcagccc cgtggaacgg atcttcatgc
     1141 ccttgacgtc agcgagtgag cggaccggtc ttaccgtgtg gagcaacccc gggccgtggg
     1201 cgtggatgta aagcactttc actcctgcaa gttctttagg ccggaatttt tcgtaaaaat
     1261 cattggcgac ccttgtggcc acctttccgt tcgggtagcc gagcggcagg tcaagggttt
     1321 ccatgacagg gaaccttcct cttgtgtagg cgaagcagga catcccgatg tcggcgatac
     1381 ctttggcaac tccatcgtat gtctggtcgg caggcgtcaa tgtcccgccc gtcaagatcg
     1441 tgatcttgac ctggccatgg gtgcgcttct cgatctcttt tgcccatgaa tcagctgcct
     1501 tggcctgtcc gtgtgtgggc gggaagaaga cgctgtaagt caatttcgtc tgtgccgcct
     1561 ggacgggctg ggtgaccgcg caggtaagca ccaatagagc tacggaaaag cagacggtca
     1621 gaaatttttt catgttccaa ttcctccttt ctatttgatg gaaggttttt ttgagacgtt
     1681 gagtttaaga atggggaagc ctgcttgccc gcgttttatg cgggcagcag gcttctatac
     1741 ttgtaggcgg gcagaagggg tgcgatgttt tgcgctaaaa agataattat cattatttgt
     1801 tagaggcctc tttcctctct tattttttcc agctcttctc tcatcttttc cgcttcggct
     1861 tttgcctttt ccagctcttc tctggtcttt atcaattctt ctttttcact ttccctttcc
     1921 tgctggacct tttccttcaa ctcatcgatg cccacataga cggcgaaagc gcctcccagg
     1981 agaaggatga gaggtatgga gcctttgagg ataacgaaga ggtccttcca ccaggcgaaa
     2041 aggccgatga gacctagcag gacagccgct acacctccaa ccaatacagt catcgatatc
     2101 tccttttttt gaaatcgctg ccggatagcc tcccgtgatc gcgcaccgct gacaaagttt
     2161 tgaaaagcac tgtgattccg cttgctaaca taaaaaaagg acggaggcaa gttgaaaatt
     2221 cccacaaata atgcttgctt aaaaggagtt attaaactat atgaaaatac taagataaat
     2281 agaagatagg gaaaggtgtt tcgacttcat gttcgaaccg gatttcgata agggaaacgg
     2341 ccttataccg gcgattgttc aggatttcga ctcgaaagaa gtacttatgc tcgcctacgt
     2401 gaaccggcag gcgtggcttt ccacccttga gacggggaag gcgacctact ggagcaggtc
     2461 aagaaactct ctctgggtca agggtgagag ttccgggaac gtgcaactca tcagggagat
     2521 cctggtagat tgcgattccg ataccctgtt gttcaaggtg gagcaggtcg gcggcgcggc
     2581 ctgtcatacg ggttatcaaa gctgtttcta ccgcaggatt tcggacggaa aggaagaaat
     2641 agtgggcaaa aaagtcttcg acccggagga tgtctacaag tgacgatggt aagactgggt
     2701 atacccaagg gaagcctgga agaaagcacc gtcgagcttt tcaagcgggc tggttggcgg
     2761 attacttacg acgccaggag ctatttcccg gacatcgacg acgacgagat ttcctgcata
     2821 cttgttaggg cacaggagat gtcccgatac gtggaagaag gtgtcctcga cgtcggtctc
     2881 accggacgcg actgggtcgt ggagaacgat tcggacgttg tagtggtgca ggaacttgtt
     2941 tactcgaagg ttacagcgcg gcaggccaga tgggtgttgg tcgtgagaga agactcgccc
     3001 atcaggtccc tcgaagacat ggaagggaag aggatctcca cggagctcgt caacttcacg
     3061 aagagatact tcagggaacg gaacatcgac gtccatgtgg agttttcctg gggcgccacg
     3121 gaggcaaaat gcgtcgaagg cctggtcgac gccatagtgg aagtgacgga gacgggaagc
     3181 accatcaggg cgaacaacct caggattgtc catgaattga tgaaaacgaa cacccagctg
     3241 atcgcaaaca aggattcctg ggccgacccc gtaaagaaaa gaaagatcga gcaggtcatg
     3301 gttcttctga acggctcact ccaggccata ggaaaggtag gaatcaagct gaacgtcgcc
     3361 cgggagaatc ttggacgggt gatgctgctc ttaccctccc tgaaagcgcc gaccgtttca
     3421 gggttgtaca atgaagattg gttcgccgtg gaaacgatcg tagagaaaaa catagtaagg
     3481 gacctcgtgc cgttgctcca ggaggcaggg gccgaaggga ttatcgagta ccccctgaac
     3541 aaggtaatct gaaaggaagg gacgatggcg agggagggca aggtatacag gaagacgtcg
     3601 gaaacggaga tttccctgga gctgaacgtc gacgttcccg gcgggagcga tgtatcgacg
     3661 acgatcccgt tccttgacca catgctccga ctctttgcaa ggcacggctt
//
LOCUS       DAOTMX010000165         3674 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_20009_length_3674_cov_3.721746, whole genome shotgun sequence.
ACCESSION   DAOTMX010000165 DAOTMX010000000
VERSION     DAOTMX010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20009_length_3674_cov_3.721746"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="P5573_05600"
     CDS             <1..205
                     /locus_tag="P5573_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HRT27569.1"
                     /translation="EARKSQVGTGDRSERIRTYNFPQGRVTDHRIGLTLYNLENILDG
                     DIQVLIDALTTHYQAEALKRSAA"
     gene            209..1078
                     /gene="prmC"
                     /locus_tag="P5573_05605"
     CDS             209..1078
                     /gene="prmC"
                     /locus_tag="P5573_05605"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HRT27570.1"
                     /translation="MAEKSRTLLDILAGAVDTLKKSGIPTPRLDAEVLLSRLLGIDRT
                     RLFVLSHTNLPYEQAEDFRRLIDRRRRGEPVAYIVGFKEFWSLPFEVTPDVLIPRPET
                     EILVEEVLRACRAFSLDEPVILEVGTGSGAVGVALARELPGARIVATDISPAALEVAE
                     RNARLNGVRERISFLCGDLFGPISGKFDIISSNPPYIPEADFSRLPLGVRGFEPSVAL
                     VGGPEGTEFQEALIKEALGYLKEGGWLLMEIGDGQGKQVEDLLSAEGDYCDVGFAADY
                     AGLLRVVRARRKG"
     gene            1082..2332
                     /gene="murA"
                     /locus_tag="P5573_05610"
     CDS             1082..2332
                     /gene="murA"
                     /locus_tag="P5573_05610"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HRT27571.1"
                     /translation="MDKIILQGGARLEGTVHVSGAKNAALPVIVSSLLTGGWNTFHNI
                     PDLVDIKTVKRLLMDFGVEFEGAEDLRVNAGNLKSFVAPYDLVRTMRASILVLGPLLA
                     RMGRARVSLPGGCAIGARPVNLHVKALQELGAEVDLADGYLEAKAPRLRGAVIYFDIS
                     TVTGTENVMMAAVLARGTTVLKNAAREPEVVNLAEVLNGMGAKISGAGSDVIVIEGVD
                     NLHPVEASVIPDRIEAGTFMIAAGITGGDVRILGCNPAHVEALTLKLRETGMEVTPVD
                     GGVRVVGSDRIESVDVTTLPYPGFATDLQAQFMALMAVGNGISVITETVFENRFMHVG
                     ELMRMGGDIVVQGNSAIVKGVKKLRGAPVMATDLRASASLIIAGLVAEGRTELSRVYH
                     IDRGYQQVEKKLSALGADIRRVRV"
     gene            2370..>3674
                     /gene="hisD"
                     /locus_tag="P5573_05615"
     CDS             2370..>3674
                     /gene="hisD"
                     /locus_tag="P5573_05615"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HRT27572.1"
                     /translation="MRTIRTQDGNFEPEFRRIKGRGGVLDRGLEAEVRQIIDDVATRG
                     DDALFEYIRRFDRLSLDSRSFVVSADEMQQALEDLDPATRDILETAAGRIEDFHQRQL
                     VGSWSYGGEDGIELGQLIVPLERVGVYAPGGLASYPSTVLMATIPARVAGVCEIYLAT
                     PSAKGEGVDRLTLAAAGLGGVRCLYRMGGAQAVAAFAYGTQTVPSVDRVVGPGNAYVA
                     MAKRMVYGAVGIDMIAGPSEVMIVCDGNVDPSWIAADLIAQAEHDEMASAILLTPAES
                     FALAVSREVERQLNTLVRGAIARRAIDEYGAVFITESLKEAVEIVNRFAPEHLELVVD
                     RPKDLLKGVRNAGAVFLGPLTPEAFGDYLAGPNHILPTGGTARFSSPLGVHDFIKRTN
                     IISFSRSSFDMYGDKAVAFAELEGLEGHGNSVRVRKGKRKGKK"
BASE COUNT          743 a          977 c         1232 g          722 t
ORIGIN      
        1 ggaggcgagg aagagtcagg tgggaacggg cgaccggagc gagaggataa ggacgtacaa
       61 cttcccccag ggaagggtga ccgaccaccg gatcgggctg acgctttaca accttgaaaa
      121 catcctcgac ggcgacattc aggtcctgat cgacgccctc acgacccact accaggcgga
      181 agcgctgaag cgctctgcgg cgtaagggat ggcagaaaaa tcacgcaccc tcctggatat
      241 tctggccggc gccgtcgata ccctcaagaa gagcggtatc cccacgccac ggctcgatgc
      301 cgaggtcctt ctgtcccgcc tcttgggcat cgaccgaaca aggctttttg tcctttccca
      361 cacaaacctt ccgtatgagc aggccgaaga cttccggcgg cttatcgacc ggcggcggag
      421 gggagaacca gttgcctata tcgtcggttt taaagagttc tggtccctac ctttcgaagt
      481 tacgccggac gtgctcatcc cgaggccgga aacggagatc ctagttgagg aagtccttag
      541 ggcatgcagg gccttttctt tggatgagcc cgtcattctc gaggtcggga cggggagcgg
      601 tgccgtcggc gttgccttgg cccgggaatt gcccggtgcg cgcatagtgg cgacggatat
      661 ctctcccgct gcactcgagg tagcggaaag aaacgctcgc ttgaacggtg taagagagag
      721 aatctctttt ctctgtggcg atttgttcgg gcctatttcg ggaaaatttg atataatctc
      781 gtccaacccg ccttacatcc cggaagcgga tttttcccgc cttcctttgg gggtgcgggg
      841 cttcgagccg tctgtcgccc tggtcggcgg gcctgaagga acggaatttc aggaagccct
      901 gataaaagaa gccctcggct acttgaaaga agggggctgg ctcctgatgg agatcggcga
      961 cggccaggga aagcaggtcg aggacctgct cagtgcggaa ggcgattact gcgatgtcgg
     1021 atttgccgcg gattatgccg gccttttgcg agttgtccgg gcgcgaagaa agggataggg
     1081 cttggataaa ataatcttgc agggcggcgc aaggcttgag gggacagttc atgtgagcgg
     1141 tgcgaagaac gcggcccttc cggtcatcgt gtcgtctctc ttgaccgggg gctggaacac
     1201 tttccacaac atccccgacc tggtggacat aaagacagtc aagcggttgc tcatggactt
     1261 cggcgtggaa tttgagggtg ccgaagacct gagggtgaat gcggggaact tgaagagttt
     1321 cgtcgccccc tatgaccttg tgaggacgat gagggcctct atcctggtcc ttggcccact
     1381 ccttgcccgg atggggcggg cgcgggtctc tttgcccggc ggatgcgcta tcggggcaag
     1441 gccggtgaac ctgcacgtca aagcccttca ggagctcggc gccgaggtcg acctcgcgga
     1501 cggttacctg gaagcgaagg ccccgcgcct cagaggtgcc gtcatatact tcgacatctc
     1561 gaccgtcacg ggcacggaaa acgtgatgat ggccgcggtc ctggcgcggg gaacgacggt
     1621 gctgaaaaac gccgcccggg agcctgaggt cgtgaacctg gcggaagtgc tgaacgggat
     1681 gggtgcaaag ataagcggtg ccggcagtga cgtgatagtg atcgagggtg tggataacct
     1741 ccatcccgtc gaggcttccg tcatcccgga ccggatcgag gccggaacgt tcatgattgc
     1801 tgccggcata acgggcgggg atgtgaggat actgggatgc aatccggcac atgtcgaggc
     1861 gctcacgctg aaactcaggg aaacggggat ggaagttacg cccgtagatg gtggggtcag
     1921 ggtcgtaggc tccgaccgga tagagagcgt ggacgtgacg acccttccgt atcccggatt
     1981 tgcgacggac ctacaggccc agtttatggc gctgatggct gtgggcaacg ggattagcgt
     2041 gatcacggag actgttttcg agaaccggtt catgcacgtc ggggaactca tgcggatggg
     2101 aggggacatc gtcgtgcagg gaaacagtgc aatcgtgaag ggtgtcaaga aactgcgcgg
     2161 ggcgccggtc atggccaccg acctgagggc gtcggcatcg ctgatcatag cggggttggt
     2221 ggcggaggga aggaccgagc tttcgagggt ttatcacatc gaccggggat accaacaggt
     2281 cgagaagaaa ctttcggccc tgggggcgga tatccgccgg gtgagggtct gagaagcggc
     2341 actggaagag taaaaaatcg gggaaaagga tgcgaaccat aaggactcag gatggtaact
     2401 ttgagccgga attcaggcgg atcaaagggc gcggtggggt cttggacagg gggctggagg
     2461 cggaggtacg gcagataatc gacgacgtcg ccacgcgggg ggacgacgcg cttttcgagt
     2521 acatccggcg attcgaccga ctttccctcg acagccggtc cttcgtcgtt tccgccgatg
     2581 agatgcagca ggcccttgaa gacctcgacc cggccacccg cgacatcctc gagacggcgg
     2641 ccgggaggat agaagatttt caccaacggc aactcgtcgg ttcgtggtcc tatggcggtg
     2701 aagacgggat cgagctcggt cagctcatcg ttcccctgga acgggtgggc gtctatgcgc
     2761 cgggcggcct ggcctcttac ccctcgacgg tcttgatggc tacgatcccg gccagggtag
     2821 cgggtgtctg cgaaatttac cttgccaccc cttctgcaaa aggggagggc gtggaccgtt
     2881 tgaccctcgc ggccgccggc ctgggaggag tccggtgtct ttacaggatg ggaggggctc
     2941 aagccgtcgc cgccttcgcc tacgggaccc agaccgttcc ctctgtcgac agggtcgtcg
     3001 gtcccggcaa tgcttacgtg gccatggcca agaggatggt ctatggagcg gtgggtatcg
     3061 acatgatcgc cggtcccagt gaggtaatga ttgtctgcga cggtaacgtc gacccgtcgt
     3121 ggatagcggc cgacctgatc gctcaggcag agcacgacga gatggcgagc gccattcttc
     3181 tcacgccggc tgagtctttt gcccttgccg tttccaggga ggtggagagg cagttgaaca
     3241 cccttgtgcg gggggcgatc gcccgccggg cgatcgatga atacggggcc gtcttcatca
     3301 cagagtcgtt gaaggaagcc gtcgagattg tcaaccgatt cgccccggag cacctggaac
     3361 tcgttgtcga taggcccaaa gatttgctca aaggggtccg gaacgccgga gccgtttttc
     3421 tgggaccgct gaccccggag gccttcggcg attatcttgc cgggcccaat cacatcctcc
     3481 ctacgggagg aacggctcgg ttttcctcgc ctctcggggt acacgatttc atcaagcgga
     3541 cgaacatcat ttctttttcc cggtcttcgt tcgatatgta cggggataag gcggttgctt
     3601 ttgcagagct ggaaggcctc gaagggcacg ggaattccgt gagggtacgg aaaggaaaaa
     3661 ggaaaggaaa aaaa
//
LOCUS       DAOTMX010000166         3645 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_20230_length_3645_cov_2.744011, whole genome shotgun sequence.
ACCESSION   DAOTMX010000166 DAOTMX010000000
VERSION     DAOTMX010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20230_length_3645_cov_2.744011"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..1427)
                     /gene="hisS"
                     /locus_tag="P5573_05620"
     CDS             complement(135..1427)
                     /gene="hisS"
                     /locus_tag="P5573_05620"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HRT27573.1"
                     /translation="MAGTIQSVRGFKDILPGDVEKWRFVEEKAREVLENFGVREIRVP
                     ILEKTELFQRGIGEATDIVEKEMYTFVDKGRESLTLRPEATASVIRAYLQHSLDARDP
                     VAKLFTIGPMFRRERPQKGRLRQFHQINVEFIGVSDALIDGEIIILLMHLLKSLGVEG
                     LHLELNSLGCDACRPSFREAIKEFLEGRKEGLCEDCLRRSEVNPLRVFDCKVEGCREV
                     MAEAPRLPDFLCDDCRSHFETVGECIRMFGIPFEVRAQMVRGLDYYTRTAFEVTTESE
                     GAQNAVAGGGRYDRLIAELGGPDIPGVGFAIGMERLVSLVPARADSFVRHPDLFIAAV
                     GETPRLRAFEICNHLRLAGIRAEMGYEGKSLKSQMKRSDKLRCSFTLIIGDREVEEKR
                     AYLRDMKKATQEAVSLESIDGIAKTISIKIAGRKEEIG"
     gene            complement(1511..>3645)
                     /locus_tag="P5573_05625"
     CDS             complement(1511..>3645)
                     /locus_tag="P5573_05625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HRT27574.1"
                     /translation="ENATEPVEGLILLSTRDTANLPDYGDYIRFKAKLKEPRNFNNPG
                     GFDYRRYLLGKGIRARGYLDKSGFVMIRSGGGNVLKARLESYRNSLRQAIRENASSPE
                     GEIIQAMVLGEQSEIPREVMDKFNRTGTSHVIAISGFNIGIIAAVSFFLARLIMRQSE
                     YLLLRLNILKVSAVFSFLPVILFAFISGLGISVLRASLMVLAFLVAACFGRGRDSFNI
                     LALTAILILTVSPLALFDVSFQLSFVAVASILFMVPVFSLYLPDGKRIEESRYPWLVK
                     ATAAAGLFLAATLSATLGTAPFIIYHFNLLSLITLIANAVIVPIMGYLVIVLGMVIIV
                     STPLSSFLTTSVVKLASSLTALSVWLADRLTNLPWAFTFVPTPTVLELGAYYVLLFCA
                     VQLLKERHGKDEATVKETRRVSAALLSASLSLILLFYAADFIYFHNRDRHPDTLRVTV
                     IDVGQGTSTLVDFPGGTKMLIDGGGFYDGNFDVGRYVLAPMLWHERVGRVDIAVLSHP
                     HPDHLNGLLYILRNFRVKEVWTNGDEGDPDTYEEFKRIIAEENIRHRVLKGRSRRHFK
                     VGDSDVHVLNLRNCPEESDTFDLNDRSLVLKVTYGKTSFLLPGDISEAAEEELVRRNG
                     PLLRSQVIVAPHHGSARSSSQPFLKAVRPETAVFSCGAGNVFRLPHPDALARYSRIGC
                     RIFRTDLNGAVTFQTDGQNITARPFRGL"
BASE COUNT          713 a         1055 c         1154 g          723 t
ORIGIN      
        1 cccgcaggtc gacaaagata acccccccgt ggtcccgacg gcggtgcgcc catcccatga
       61 ggatgacttc cttccccgcg tcggtggccc ggaggtcgcc gcagtagtgg gttcttttat
      121 cggtcgtgat gaaatcagcc aatttcctct ttccttcctg ctatcttgat cgatatggtc
      181 tttgcgatac cgtcgatgct ctcgaggctg acggcctctt gcgtcgcctt cttcatgtct
      241 ctcaggtagg cccttttttc ttcgacctcc cggtcgccga ttatcagcgt aaaggagcag
      301 cggagcttat cggaacgttt catctgactt ttcagactct tcccctcgta gcccatctcg
      361 gcccggatgc cggccaggcg caggtgattg catatctcga aggcccggag ccgcggcgtc
      421 tcacctacgg cggcgatgaa gaggtccgga tggcggacga aggagtcggc cctcgccggg
      481 accagggaga cgagcctttc cataccgatc gcgaacccca ctccggggat gtcggggcct
      541 ccgagttctg cgatcaaacg gtcatagcgg ccaccgccgg cgacggcgtt ctgggcaccc
      601 tcggattccg tcgtgacctc gaatgccgtt ctcgtgtagt aatcgaggcc gcggaccatc
      661 tgcgccctga cctcgaaggg gatgccgaac atcctgatgc actctccgac cgtctcgaag
      721 tggctgcggc aatcgtcgca gaggaagtcg gggagccgcg gcgcttccgc catgacttcc
      781 cggcaccctt ccaccttaca gtcgaagacc cgcagggggt tcacctccga cctccgcagg
      841 cagtcttcac agaggccctc tttcctgcct tccaggaact ccttgatcgc ctcccggaac
      901 gagggtctgc aggcatcgca gccgagggaa ttgagctcca ggtgcagccc ttcgacgccg
      961 aggcttttca gcaggtgcat cagcaggatg attatctcgc cgtcgatgag ggcgtcgctc
     1021 acgccgatga actcgacgtt tatctggtgg aactggcgga gtctgccctt ctgcgggcgc
     1081 tcccgccgga acatcgggcc gatcgtgaag agcttggcca ccgggtcccg ggcgtcgagg
     1141 gaatgctgga ggtaggcgcg gatgaccgag gccgtcgcct ccgggcggag cgtgagggac
     1201 tcccgccctt tgtccacgaa ggtgtacatc tccttttcga cgatgtctgt cgcctccccg
     1261 atcccgcgct gaaaaagctc cgtcttctcg aggatcggga cccttatctc gcggaccccg
     1321 aaattttcca ggacctcacg ggccttttcc tcgacgaacc gccacttttc cacgtcgccg
     1381 ggcaggatgt ctttgaaccc ccggacagat tggatggtac cggccatggt tggtgattga
     1441 aactcctgat ttttttgaac tttgctgcat accacggaaa gaggccaaag gcaatataaa
     1501 tatcgccctg tcagagaccc cggaaaggcc gggcggtgat attttgccca tcggtttgaa
     1561 aggttacggc cccgttcagg tccgtgcgga agatgcgaca accgatacgg ctgtaccttg
     1621 ccagggcatc gggatggggt aggcggaaga cgttgccggc cccgcaggaa aagacggcag
     1681 tctcgggcct gacggccttg aggaatggct gggagctcga ccttgcgctg ccgtggtggg
     1741 gcgcaacgat gacttgactc cgcagcaatg ggccgttccg cctgacgagc tcctcctcgg
     1801 cggcctcgga gatgtcgccg gggaggagaa agctcgtctt tccgtaggtc accttgagga
     1861 cgagggagcg gtcattcagg tcgaaggtgt cgctttcttc gggacagttg cggaggttca
     1921 ggacatgcac gtcggaatcg ccgaccttga agtgtctccg tgaccttccc ttcagcaccc
     1981 ggtggcggat gttctcctcc gctattatcc gtttgaactc ttcgtaggtg tccgggtccc
     2041 cctcgtcgcc gttcgtccac acctctttta cccggaaatt cctcaggatg tacaggaggc
     2101 cgttcagatg gtcgggatgg gggtgggaaa ggacggcgat gtccacccgg ccgaccctct
     2161 cgtgccagag catgggtgcc aggacatagc ggccgacgtc gaagtttccg tcgtagaacc
     2221 cgccgccgtc gatcagcatc ttcgtccccc cggggaaatc gacgagggtg gaggttccct
     2281 ggcccacgtc gatcaccgtg accctgagcg tgtcggggtg gcggtccctg ttgtggaagt
     2341 agataaagtc ggctgcataa aagagcagga tgaggctcag ggaagcgctc agaagcgccg
     2401 cagacacccg gcgcgtctcc ttcacggtgg cctcgtcctt tccatgcctt tctttgagca
     2461 actgcacggc gcagaaaagg aggacgtagt aggcgccgag ttcgaggacg gtcggcgtcg
     2521 ggacgaaggt gaaggcccag ggaaggttgg tcagccggtc cgcgagccag accgacaggg
     2581 ccgtcaggga ggaagcgagc ttgaccaccg aggtcgtcag gaaagaagag aggggcgtgg
     2641 agacgatgat taccatcccg aggacaatca cgagatagcc catgatcggg acgatgacgg
     2701 cattggcgat gagggtgatc agcgaaagga ggttgaaatg gtagattatg aagggcgccg
     2761 tgccgagggt cgcgctcaaa gtagccgcga ggaaaaggcc ggccgctgcc gtcgccttca
     2821 caagccacgg ataccggctc tcctcgattc tctttccgtc gggaaggtac agggaaaaga
     2881 cgggaaccat gaagaggatg gatgcgacgg cgacgaaaga gagctggaag gagacgtcga
     2941 agagggcaag cggcgagacg gtgaggatga ggatcgcggt cagggccagg atgttgaatg
     3001 aatcgcgccc ccgtccgaaa caggccgcga cgaggaaggc gaggaccatc agggatgccc
     3061 tcaagacgga gattccgagg ccggagatga aggcgaagag gatgacgggg agaaacgaaa
     3121 agaccgccga gaccttgagg atgttgagcc tcagcagtag gtactccgat tgcctcatga
     3181 tgagtcgcgc aaggaagaag gaaacggccg cgattatccc gatgttgaac cccgatatgg
     3241 cgatgacatg agacgtcccc gtccggttga atttgtccat cacttcgcgg ggtatctcgc
     3301 tctgctcgcc gaggaccatg gcctggatga tctcaccctc gggggaagag gcattttctc
     3361 tgattgcctg gcgcaagctg ttcctgtagc tttcgaggcg tgccttcagt acatttccgc
     3421 cccccgacct gatcatgacg aaaccggact tgtccaggta acccctcgcc cggataccct
     3481 tacccaaaag gtaacgccga taatcgaaac caccggggtt gttgaagttg cggggttcct
     3541 tgagctttgc cttgaagcgg atgtaatcgc cgtagtccgg gaggttggcg gtgtccctcg
     3601 tggaaaggag gatcaggcct tcgaccggct cggtagcgtt ctcct
//
LOCUS       DAOTMX010000167         3621 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_20384_length_3621_cov_3.266685, whole genome shotgun sequence.
ACCESSION   DAOTMX010000167 DAOTMX010000000
VERSION     DAOTMX010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20384_length_3621_cov_3.266685"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..994
                     /locus_tag="P5573_05630"
     CDS             <1..994
                     /locus_tag="P5573_05630"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HRT27575.1"
                     /translation="RMIPIKQTFQKITRLVRDLSKATGKKVNVAMTGEETEIDRNMVE
                     EIYNPLVHMVRNAVDHGVEKPAERMMAGKPEAGTISLKAYHRGGTIVIEIADDGKGLD
                     AQKILDKAVGNGLIEAGAGLTEQEIFRLIFLPGLSTASTVSDVSGRGVGMDVVKKAID
                     KLRGKVDIQSVKGKGTTFIASFPLTMAIIDGMIVRVGKEKYIVPTSAVRLLLRPDRSE
                     CSTVAQKAEMVNVRGTLMPLIRLHELFGIDGERKKPWESIVMVVDSDNRSKCVMVDEI
                     LGKEEVVIKGLGDGMKKAKGVSSGAILGDGNIGLILDPEGLFDLSETAMGAKRR"
     gene            1027..1413
                     /locus_tag="P5573_05635"
     CDS             1027..1413
                     /locus_tag="P5573_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRT27576.1"
                     /translation="MEKKISILVVDDFATMRKVIRNLLKQVGYENIVEAENGEAALSV
                     LKSRKVDLIISDWNMPTMSGYELLKAVRADDELQKTPFLMVTAEALKDNVVQAVKAGV
                     SNYIVKPFTAEVLNDKISQIFKKTAS"
     gene            1444..1905
                     /locus_tag="P5573_05640"
     CDS             1444..1905
                     /locus_tag="P5573_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HRT27577.1"
                     /translation="MDVRFINPFLEGTMSVLRTMAFIEPRPGKPYLKKNNVAQGDISG
                     IIGLTGDVRGSLALSFSERCIMKIVSNMLGEEIKSMNGDIKDAVGEITNMVSGVARKK
                     LEGMDLTISAAIPTVVSGKNHSILHVLGGPSIIIPFETDHGPFVVDVCLNA"
     gene            complement(2113..3573)
                     /locus_tag="P5573_05645"
     CDS             complement(2113..3573)
                     /locus_tag="P5573_05645"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRT27578.1"
                     /translation="MKDFNARYTLKTKALMLLGIVVVVSLCMAGYTVMTVRNNIITTA
                     HDKLKSVAALTLAILNDKYPGEWSIRDGKLFKGDTPMNENYECIDRISEMTGGDTVTI
                     FQADTRIATSMKNSSGERPIGTKADGVVVDAVLKNGRSYIGKADVTGAPYQSFYTPLR
                     NNGGETVGMLAVAVPNANYDRIAFDTLINLLVMGIAAFVIIFAGGFMIYRSITRPIER
                     IAGGLNEGAEQVASASNQVSSSSQVLAEKASEQARYVSEAVASAEEMVALMEKNEAYL
                     RELDGFRESTLQGMKNSNKAIRKNKECMEQITSSSEKTAQIIKKIDEIAFQTNLLALN
                     AAVEAARAGASGAGFAVVADEVRNLARRSAEAAKETETLLGETQRSVQEGSELVNKTM
                     AEFYTMGDLGKSTSDKIKEVEMISASVSEAIRRVRNIIEKIDGITQENAASAEESASA
                     AEEMTAQSAQMRSHVTDLLSVVSGNHRNGKGHAAGF"
BASE COUNT          806 a          913 c         1047 g          855 t
ORIGIN      
        1 gaggatgata ccgatcaaac aaacttttca gaagataacc cgcctcgtga gggatctctc
       61 gaaggccacc ggcaaaaagg tcaacgttgc gatgacgggg gaagagacag aaatagaccg
      121 gaacatggtg gaagagatat acaaccccct agttcacatg gtgcgcaatg ccgtggatca
      181 cggggtcgaa aagcctgcgg aacggatgat ggcagggaag cccgaagcgg ggaccatcag
      241 cctgaaggcc taccatcgtg gtggtacgat cgtgatcgag attgccgacg acggcaaagg
      301 tttggacgcg cagaagatat tagataaggc cgtggggaac ggactcatcg aagccggtgc
      361 cggcctcacg gagcaggaga tattccgctt gattttcctg cccgggctgt cgacggcttc
      421 caccgtctcg gatgtctccg ggaggggggt tggtatggat gtcgtgaaga aggccatcga
      481 caaattgagg ggcaaggtgg acatccagag cgttaaaggg aagggtacga cgttcatcgc
      541 gagcttccct ctcacgatgg ccatcatcga cggcatgata gtcagggtcg ggaaggagaa
      601 gtatatcgtt cccacgagtg ccgtcaggtt gctgctaagg cccgaccggt cggaatgcag
      661 cactgtcgcg cagaaggccg agatggtcaa cgtgaggggg acgctcatgc cccttatccg
      721 actccatgag ctgttcggga tagacggaga acgaaagaaa ccctgggaat ccatcgtcat
      781 ggtcgtggac agcgacaacc gttcaaaatg tgttatggtg gatgagatac tcggtaaaga
      841 agaagtggta atcaagggtc tcggcgacgg catgaaaaag gcgaaaggcg tttccagcgg
      901 cgccatcctg ggcgacggca acatcgggct cattttggac cctgaaggct tgttcgacct
      961 gagtgagacg gccatgggcg caaagcgacg ttgagaagct tgagggcact attttttgga
     1021 ggtcttatgg agaagaagat ttcgattctt gtcgtggatg attttgcgac gatgcgcaag
     1081 gtcatccgta atctgttgaa gcaggtgggg tacgaaaaca tagtcgaggc cgagaacggg
     1141 gaggcggcat tatcggtgct caagtcccgt aaggtcgact tgataatatc ggactggaat
     1201 atgccgacca tgtcggggta cgagctgctc aaagcggtcc gggccgacga cgaactgcag
     1261 aagacgcctt tcctgatggt gacggcggag gcattgaagg acaacgtagt ccaggccgtc
     1321 aaggccggcg tcagcaatta catcgtaaaa cctttcactg ccgaggtgct gaacgacaag
     1381 atcagccaga tattcaaaaa aacggcttca tgaatgtgct gatgctccgg aggagaatga
     1441 aaaatggacg tgaggtttat caatcctttt cttgaaggaa cgatgagcgt tttgaggacg
     1501 atggccttta tagaacctcg ccccggaaag ccttatctta agaagaataa tgtcgcccag
     1561 ggggatatat cggggataat agggctgact ggtgacgtca ggggatcgct tgccctgagt
     1621 ttcagcgagc gctgtatcat gaagatcgtg tcgaacatgt tgggcgaaga gataaagtcg
     1681 atgaacgggg acatcaaaga cgccgtgggc gagataacca acatggtgtc cggcgtggcg
     1741 agaaaaaaat tggaagggat ggatctcacg atatcggctg ctatcccgac tgtcgtctcc
     1801 gggaaaaacc actccatcct tcatgtcttg ggcggcccga gcatcatcat ccccttcgaa
     1861 acggatcacg gacctttcgt cgtcgacgtc tgcctgaatg cataagatct taccgttcgg
     1921 ttaaggtatt acgtgatggg ctcgcaaaaa gtcgagaatt acccatttcg tcattccggc
     1981 tagttacccc gatgaaaatc ggggtggaat ccagtatctt cgatatgttc tggatgcccc
     2041 cgtacctgat acggtggcat ccaatgttct caggcggttc gggatttata cttccatcgg
     2101 gagtaacctc tgtcagaacc ccgccgcgtg tcccttcccg ttccggtggt tgccgctgac
     2161 gaccgacaga aggtcggtta catggctcct catctgtgct gattgagcgg tcatctcttc
     2221 ggcggcactg gccgattctt ccgcactggc cgcattctcc tgggttatgc cgtctatctt
     2281 ttcgatgatg ttccggaccc tacggatggc ttctgatacg ctcgcgctga tcatctccac
     2341 ctctttgatc ttatcggaag tcgattttcc gaggtccccc atagtgtaaa actccgccat
     2401 cgtcttgttg accaactcgg agccttcttg gacgctcctc tgggtttcac ccagcagtgt
     2461 ttccgtttcc tttgccgcct ccgccgaacg ccgggcgagg tttctaactt catcggcgac
     2521 cacggcgaaa ccggcaccgc ttgccccggc cctggccgct tcgacggcgg cattcagggc
     2581 aagcaggttg gtctggaagg cgatttcgtc aatctttttg attatttgcg cagttttttc
     2641 gctggagctt gttatctgct ccatgcactc cttgttcttc cttattgcct tattgctgtt
     2701 tttcatgccc tggagggtgc tttcgcggaa gccgtcgagt tcgcgcaggt aggcttcgtt
     2761 cttttccatc agggcgacca tctcctcggc ggaagcgacg gcctcgctga cataccttgc
     2821 ctgctccgat gccttttcgg ccaggacctg actcgaagaa gagacctgat tcgatgcgga
     2881 ggcgacctgc tcggcccctt cgttcaggcc gccggcaatg cgttcaatcg ggcgggtgat
     2941 agacctgtag atcatgaacc cgccggcgaa gatgatgacg aatgcagcta ttcccataac
     3001 aagcaagttg atcagggtgt cgaaggcgat ccggtcataa ttcgcgttgg ggacggccac
     3061 ggcaagcatc ccgacggttt caccaccgtt gtttctaagg ggtgtgtaga aagattggta
     3121 cggcgctcca gtaacgtccg cctttccgat gtacgagcgt ccgtttttca gtacggcgtc
     3181 gaccacgaca ccgtccgcct tcgttccgat cggtctctcc ccggaggagt ttttcatgga
     3241 ggtggcgatt cgggtgtctg cctggaaaat agtgaccgta tctcctcccg tcatttcgga
     3301 gatacggtcg atacattcat agttttcgtt catcggcgtg tcgcccttga agagtttgcc
     3361 gtcccggatc gaccactcgc cgggatactt gtcgttgagg atggccaagg tcagggcggc
     3421 gacgcttttc aatttatcat gggccgtcgt gataatattg tttcgtaccg tcatgacagt
     3481 gtagccagcc atacacaaag agacgacgac tacgattccg aggagcatca aggccttggt
     3541 ttttagtgtg taacgggcgt taaagtcttt cattttcccc tcctatctaa aaagaagttt
     3601 ccattatgcc tcgagatctt t
//
LOCUS       DAOTMX010000168         3608 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_20467_length_3608_cov_4.414016, whole genome shotgun sequence.
ACCESSION   DAOTMX010000168 DAOTMX010000000
VERSION     DAOTMX010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3608
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20467_length_3608_cov_4.414016"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="P5573_05650"
     CDS             complement(<1..521)
                     /locus_tag="P5573_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HRT27579.1"
                     /translation="MNGIKLIFVTILLFLMTVPAQGARIKDIAGIGGVRDNQLVGYGL
                     VAGLAGTGDDVKNGFTKETIVNMLIRQGISMTGRDLKADNVAAVMVTAKLPPFAKVGT
                     KIDVNVSSIGDAKSLQGGTLLMTHLKGSDGEVYAVAQGPVTLGGFAASGASGSSVGKN
                     HTTAGFIANGAFV"
     gene            complement(577..1272)
                     /locus_tag="P5573_05655"
     CDS             complement(577..1272)
                     /locus_tag="P5573_05655"
                     /inference="COORDINATES: protein motif:HMM:NF014195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HRT27580.1"
                     /translation="MGRDLTIGKGAARRAIFVVVLCIMAGCSQNLYNLNDKTPLDVPP
                     PQVQRPVGTIWPGENSANTLFADRKARHVNDIVTIIIAESSTGANNAKTTTSRDADTK
                     ANIGGAFQTGPDRTLLSKYDGGGGSKNSLKGDGATSRNSNLTGRISARVVKILSNGNL
                     YIEGKRQLTINAEDQYLIVSGVVRPEDISIENTVASYSISDAKIHYAGKGVVDDKLRP
                     GWLTRLVDWVWPF"
     gene            complement(1302..2036)
                     /gene="flgA"
                     /locus_tag="P5573_05660"
     CDS             complement(1302..2036)
                     /gene="flgA"
                     /locus_tag="P5573_05660"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HRT27581.1"
                     /translation="MAMTVERTKNNCRLLVAAAVAVSLLIGGQLEAATVSAVAIKDAV
                     RTYIEKHMPWPKGHMRVFFPSQVRDLQLPGRKIDLEVRGKRNENFVGDSFYTVSFYED
                     DVLLRQETVRAKIEVFHDVALSVRALEKGREIRSEDVKFEGRWFNEVPANAVTNFDDF
                     VGKQLTTSVRPNCEITRNMLRSYPLVRRGKVVRVVLESGSMTILTVGLPEEDGSRNDV
                     VRVKNLSSNKVIYARVVEDNTVRVDF"
     gene            complement(2055..2837)
                     /gene="flgG"
                     /locus_tag="P5573_05665"
     CDS             complement(2055..2837)
                     /gene="flgG"
                     /locus_tag="P5573_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HRT27582.1"
                     /translation="MIRALWTAATGMSAKQTCLDVVANNLANLSTVGFKKSRAEFQDL
                     MYQIQKRSGFETSSGIQSPIGIEIGMGSKLTAVQKFFTQGDYVQTGNDFDWSIEGDGF
                     FQVDDNGRTLYTRAGNFKKSKDGFLVTADGLKLIPNVTVPEETVTFTIDAGGMWTALD
                     GKGAKLGSGQLEIAKFVNPAGLTSLGHNLFEESEGSGPAKTGKPAQDGYGSISQHFLE
                     MSNVNSVDEMIYMIEALRAYEMNSKAIQTADQMLQTVSNLKR"
     gene            complement(2884..>3608)
                     /gene="flgF"
                     /locus_tag="P5573_05670"
     CDS             complement(2884..>3608)
                     /gene="flgF"
                     /locus_tag="P5573_05670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HRT27583.1"
                     /translation="ARFMAVGGIGELVDATNRVMPRLDSIANNISNMGTSGFKADRLY
                     YLKEKDPSSGGAGMKTTPLLVVDFSQGSLAATGNPLDLAIKDDGFFVVKTKNGEAYTR
                     KGNFTVNKNNELVTQDGDLLVGESGKITLNGNNVVVSENGEVSVDGNRVGKLKIVKFA
                     KPSLLVRDGNGLFSDPGGAGLGMNDKPEVQAGFLEMSNVQSIREMVEMINVQRTVDIY
                     QKGIQTISEQDKLSTSRVGRVG"
BASE COUNT          663 a         1117 c          924 g          904 t
ORIGIN      
        1 tccacaaagg ccccattggc gatgaagccg gcggtcgtgt ggttcttgcc cacagagctg
       61 ccggatgcgc cgctggccgc aaagcctccg agggtgactg gtccctgggc gactgcgtag
      121 acctcgccgt cactcccctt gaggtgagtc atcagaagag tccccccttg caagcttttc
      181 gcatcgccga tggaggagac attcacgtcg atcttcgttc cgaccttggc gaagggagga
      241 agcttggccg tcaccatgac tgcagccacg ttgtccgcct tgaggtcccg acctgtcatc
      301 gaaataccct gtctgatcag catgttgacg atggtttcct ttgtaaaccc attcttgacg
      361 tcgtcgccgg tgccggcaag tcctgccacc agtccgtagc cgaccaactg gttgtccctg
      421 accccgccta tcccggcgat gtcttttatc ctggctccct gggccggaac ggtcattaag
      481 aaaagaagga ttgtcacgaa tatcaactta atgccgttca tctcgctttt ctcctcttcc
      541 gccgttcttt cccgtctttc tttatccgcc ccatcatcag aacggccaca cccagtcaac
      601 caggcgggtc agccaccccg gcctcagctt gtcgtccaca actcccttgc ccgcgtagtg
      661 tatcttggca tcagatatgc tgtaggaagc gacggtgttt tcgatgctga tgtcttccgg
      721 acggacaacc ccggacacta tgagatattg gtcctcagca ttgatggtca actgtctttt
      781 accctcgata tagaggttcc cgttgctcag tatcttaacc accctggcgg agattctgcc
      841 cgtcaaattg ctgttcctgc tcgtggcgcc gtctcccttg agggagtttt tcgacccgcc
      901 gcctccgtcg tacttagata gaagcgtgcg gtccgggccg gtctgaaagg cgcctccgat
      961 attggccttc gtgtccgcat ccctgctcgt cgtggtcttt gcgttgttgg cgccggtcga
     1021 tgattccgcg atgatgatag tgacgatgtc gtttacgtga cgggcctttc tgtccgcaaa
     1081 aagggtgttg gccgagttct cgcccggcca gatggttccc accggacgct gaacttgcgg
     1141 cggcggcacg tcgaggggcg ttttgtcgtt aaggttgtaa agattctgcg aacaaccggc
     1201 catgatacag aggacgacaa cgaagatcgc ccgccttgcc gccccttttc cgattgtcaa
     1261 gtcccttccc acttttcccg accctccgtc tctaccgaaa atcagaaatc gactcgcacg
     1321 gtattgtctt cgacgacccg ggcatatatg accttattcg aagagaggtt cttaaccctg
     1381 acgacgtcgt tgcggctgcc gtcctcttcg ggcaatccca ctgtcagtat ggtcatcgag
     1441 ccgctctcaa ggacgaccct caccaccttt ccccttctta cgaggggata gcttctcaac
     1501 atattcctgg tgatctcaca attcggcctg acgctcgtcg taagttgttt ccctacgaaa
     1561 tcgtcgaaat tggtcaccgc gttcgccggg acctcgttga accagcgacc ctcgaacttg
     1621 acgtcctcgc tccttatctc acggcctttc tccagtgccc tgacgctgag agcgacgtca
     1681 tggaaaactt caattttcgc acgcaccgtt tcctgccgca acagcacgtc gtcttcgtaa
     1741 aaactcacgg tatagaaaga atcgccgacg aagttttcgt tcctttttcc gcggacctca
     1801 aggtcgatct tcctgccggg aagctggaga tcgcggacct gggaggggaa aaagacccgc
     1861 atgtgtcctt tcggccaggg catatgcttt tcgatgtagg tcctgacggc atctttgatc
     1921 gcaaccgcag agacggtcgc cgcctcgagt tgaccgccga tgagaaggct taccgcaacg
     1981 gcggcggcga cgagcagccg gcagttattt ttcgttcttt cgaccgtcat agccatgccc
     2041 cgctctcttt cgttttaccg cttaaggttg cttacggtct gaagcatctg gtcggccgtc
     2101 tggatggcct tgctgttcat ctcataggcc cttagggcct ctatcatgta tatcatctcg
     2161 tcaaccgaat tcacgttgga catctccaga aaatgctggg atatggagcc gtaaccgtct
     2221 tgagcgggct ttcccgtctt cgccggtccg gaaccctccg actcctcgaa aaggttatga
     2281 cccaggctgg tcagcccggc ggggttcacg aacttggcga tctcaagctg gccgctcccc
     2341 agtttagcgc ctttaccgtc cagggccgtc cacatcccgc cggcgtcgat cgtaaaggta
     2401 accgtttcct cgggtaccgt gacgttgggg atcaacttga ggccgtcggc ggtgacgagg
     2461 aaaccgtcct tgctcttctt gaagttcccc gcccgggtgt agagcgtcct gccgttgtcg
     2521 tcgacctgga aaaagccgtc cccttcgatc gaccagtcga agtcgtttcc cgtttgaaca
     2581 taatcgccct gggtgaaaaa tttctgcact gccgtgagtt tcgaacccat cccgatctcg
     2641 atgccgatgg ggctctgtat gccgctcgac gtctcgaacc ccgatctttt ctgtatctgg
     2701 tacatgaggt cctggaactc cgcccttgat ttcttgaacc cgacggtgct gaggttggca
     2761 aggttgttag ccaccacgtc caggcaggtc tgcttcgcgc tcatccctgt cgccgccgtc
     2821 caaagtgccc gaatcatcgt ttccctcctg ttttcctact ccaaagtgtt cgcgaaccgc
     2881 ggatcaccct acccgaccga cgcggctcgt cgacaactta tcctgctcac tgatcgtctg
     2941 gatgcccttc tggtaaatat cgacggtgcg ctggacgttt atcatctcca ccatctccct
     3001 gatgctctgg acgttcgaca tctccaagaa gcccgcctgt acttcaggct tatcgttcat
     3061 cccgaggccg gcgccgccgg ggtccgagaa gaggccgttg ccgtccctca ccagcaaaga
     3121 cggcttggcg aatttgacga tcttcaattt tcctacccgg ttgccgtcga cactgacctc
     3181 cccgttttca gaaacaacga cgttgttacc gttcaacgtg atctttcccg attcgccgac
     3241 gaggaggtcg ccgtcctgcg tcacgagctc attgttcttg ttgacggtga agttcccctt
     3301 tctcgtgtat gcctcaccgt tttttgtctt gacgacaaaa aaaccgtcat ccttgatggc
     3361 aaggtccagc gggtttcccg tcgccgccag tgacccctga gaaaaatcga cgaccaaaag
     3421 cggggtcgtt ttcattcccg cgccacccga agaagggtcc ttttctttca ggtagtagag
     3481 cctatcggcc ttgaagccgg aggtgcccat gttggagatg ttgttcgcga tggagtcgag
     3541 tctcggcatg acacggttgg tggcgtcaac cagttcccct attcctccga cagccatgaa
     3601 acgcgccc
//
LOCUS       DAOTMX010000169         3598 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_20551_length_3598_cov_3.533164, whole genome shotgun sequence.
ACCESSION   DAOTMX010000169 DAOTMX010000000
VERSION     DAOTMX010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20551_length_3598_cov_3.533164"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1621)
                     /locus_tag="P5573_05675"
     CDS             complement(<1..1621)
                     /locus_tag="P5573_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HRT27584.1"
                     /translation="MKRCFAGFSLLLILVIIPFMGCSGSKGVDEERGPTADTGPAYGD
                     VLVEGSIGDASNLIPILASDNTSHSIAALIYNGLVKYDKDLNVVGDLAESWEVSQGGL
                     VINFHLRKGIRWQDGKPFTADDVLYTYRVTIDPKTPTAYSGDFLKVKKAEVIDPYTFR
                     ATYDRPFAPALTSWSTAILPKHLLEGADITRTPLSRRPVGTGPYMIKEWVTGQKIVLV
                     SNPDYWEGRPYIDGYIMRIIPDMATMFLELRAGGIDRMDLTPLQFTRQTETGFFRKNF
                     RKYRYLSFSYTYLGYNLKNPLFADKRVRQAIAYAIDKDEIVQGVLLGLGKEATGPYKP
                     GTWAYNPHVRRYPYDPAKARELLAEAGWTDRNGDGFLEKDGKAFSFEIITNQGNEVRK
                     KTAEIIQRRLADVGIEAKIRIIEWAAFVKEFINKRKFDVTILGWTIPLDPDIYDVWHS
                     SKTGPDELNFISYKNAEVDRLLERGRSTFDMKLRKKCYYRVQEILAEEQPYTFLYCPD
                     ALPIISARFRGIEPAPIGIGHNFIKWYVPREE"
     gene            complement(1653..2477)
                     /locus_tag="P5573_05680"
     CDS             complement(1653..2477)
                     /locus_tag="P5573_05680"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRT27585.1"
                     /translation="MTGEKDSSRHPFYITVKGCRLAAEVIKPVGFSPDNRLPTLLFLH
                     EGLGSIGQWKDFPLVLSKAAHLQALIYDRRGHGKSDPLGGKREVNYLHEEALEYLPQV
                     MKEMGIKKAILVGHSDGGSIALIYAATHPGNVLGVITEAAHVFVEDITLRGIRKAVRV
                     YETSDLRERLARYHGNNTDRVFRGWADTWLAPEFRNWNIVEYLKKVTAPVLAIQGFDD
                     EYGTPAQVQTIVQKVSGRAEGKVIFDCGHTPHDQARERVLSEMSRFVASLVKRKIR"
     gene            2705..3490
                     /gene="xth"
                     /locus_tag="P5573_05685"
     CDS             2705..3490
                     /gene="xth"
                     /locus_tag="P5573_05685"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HRT27586.1"
                     /translation="MNPFKVATYNVNSIRSRIPLVIAWLKKNRPAVLCMQETKVENDS
                     FPAKEFHEAGYHVVFSGGSRYNGVAVASLEEPRDVCFGFEDGGPPDRDRLIRGVFSGI
                     PILNAYVPQGTSREDPRFQYKLEWFRRLLALLNRFYSSKQPLVCCGDLNVAREEIDVH
                     NPKRLLGHVDFNPEVWEAFDSLKAWGFVDVFRKFHAGEPGHYTFFDYRVPRSVERGLG
                     WRVDHILATPIMAEKAVNAYIDMEARRAEKPSDHTVLVAEFKT"
BASE COUNT          752 a          927 c         1028 g          891 t
ORIGIN      
        1 gctcctcccg gggaacatac cacttgatga agttgtggcc gattccgatg ggggctggtt
       61 ctatccctcg gaatcttgcg cttatgatcg ggagcgcatc cgggcagtac agaaaggtat
      121 aaggttgctc ctcggcaagg atctcctgaa ccctatagta gcatttcttc cgtaacttca
      181 tgtcgaaggt gctccgtccc ctttccaaga gacggtctac ttccgcgttc ttgtaagaga
      241 taaagttcag ttcgtctggt cccgttttgc tcgagtgcca cacatcgtaa atatccgggt
      301 cgagggggat cgtccagccg aggatggtga catcgaactt ccgcttattg atgaactcct
      361 tgacgaaggc cgcccattcg atgatgcgga tcttggcctc gatcccgacg tcggcgaggc
      421 ggcgttggat gatctctgct gtctttttcc tgacctcgtt tccctgattg gtgatgattt
      481 cgaacgagaa ggcctttccg tccttttcca ggaacccgtc tccgttccgg tcggtccagc
      541 cagcttcggc aagaagctcc cgggccttgg ctgggtcgta ggggtatcgc ctgacatggg
      601 ggttgtaggc ccaggtgccg ggtttgtaag gtcccgtcgc ctccttccct aatccaagaa
      661 ggacgccttg tacgatctcg tccttgtcga tggcgtaagc gatggcctgt ctgacgcgct
      721 tatcggcgaa gaggggattt ttcaggttat aacccagata ggtgtaggag aacgacagat
      781 atcggtactt tcggaagttc ttcctgaaga atcctgtttc cgtctgccgt gtgaattgca
      841 gcggcgttag gtccatccgg tctatacccc ccgccctaag ttcgagaaac atagtggcca
      901 tgtcggggat tatccgcatg atgtagccgt cgatgtaagg ccttccttcc cagtagtccg
      961 ggttggagac aaggacgatt ttctgccccg taacccactc cttgatcatg taagggccgg
     1021 ttcctacggg acgtcgggaa agaggtgtcc gggtgatgtc cgcgccttcg aggagatgtt
     1081 taggtagaat cgctgtgctc cagctcgtga gggccggcgc gaagggtcta tcgtaagtcg
     1141 cccggaaggt gtacgggtct atcacctcgg cctttttcac cttgaggaag tctcccgaat
     1201 atgcggtcgg ggtcttcggg tcgatggtga ccctgtacgt gtaaagcaca tcgtcggccg
     1261 taaaaggctt accgtcttgc catcttatgc cctttctcag gtggaaattg atgacgagcc
     1321 ctccctgcga tacctcccac gattcggcca ggtcgccgac cacgttcagg tctttgtcgt
     1381 atttgacgag gccgttgtat ataagggccg cgattgagtg cgatgtgtta tcggaggcaa
     1441 ggatgggtat gaggttgctc gcgtctccga tggaaccttc cacgaggacg tcgccgtaag
     1501 ccgggcccgt atccgccgtt gggcccctct cttcatcgac gcccttcgac ccggaacaac
     1561 ccataaacgg tatgatgacg aggatcagca ggagagagaa tcctgcaaag catctcttca
     1621 tgacaacatg ctatctcaca gggttatgat tatcaacgga tttttctctt cacgagagag
     1681 gcgacgaacc gtgacatttc ggagaggacc cgctcgcggg cctgatcatg gggggtatga
     1741 ccgcaatcga aaatgacctt gccttccgct cggccggaaa ctttttgcac gatcgtttgc
     1801 acctgggcgg gcgttccgta ttcatcgtcg aatccttgga tggcaaggac gggtgccgtg
     1861 acttttttta ggtattcgac gatattccag ttgcggaact ccggcgccag ccaggtgtcg
     1921 gcccatcccc taaacacccg gtccgtgttg tttccgtggt aacgggccag gcgttcgcgg
     1981 aggtccgacg tctcatagac gcgtaccgcc ttcctgatac ctctgagcgt tatgtcctcg
     2041 acgaagacgt gagccgcctc ggtgatcacg ccgaggacat tcccaggatg ggtggcagca
     2101 tagatcagcg caatggatcc gccgtcactg tgcccgacca gtatggcctt tttgatcccc
     2161 atctctttca tgacctgcgg aagatattcc agtgcttcct cgtggagata gtttacttct
     2221 cttttccccc cgagcgggtc ggatttgccg tgaccccggc ggtcatagat gagggcctgg
     2281 agatgagccg ccttgctgag aacaaggggg aaatccttcc attggccgat gctacctaag
     2341 ccctcgtgga gaaaaagcag ggtgggtaac cggttgtctg gggagaatcc gacgggcttg
     2401 atgacctcgg ctgcaagacg acaacccttt accgtgatat agaaaggatg ccgggagctg
     2461 tctttttctc ctgtcatagt actaacctgt ccgtcaaaat gaaaccttta ttcaaattta
     2521 tgccaaaaga ctgcccacga gagccgctta tgatattttt cttatgctaa agagccggat
     2581 taatcaagcc ggaattgtca gctttttatt ctatcggtaa gagtaccttg tggggtgttg
     2641 cgagccgtga tattagaaag cacgggtcgc tttatcccgt tgcctataca gaagaggatg
     2701 taagatgaac cctttcaaag tcgccacata caatgtgaat tctatccgtt ctcgcatacc
     2761 ccttgtgatc gcatggctga agaaaaaccg gccagccgtt ctatgtatgc aggaaaccaa
     2821 agtggaaaac gacagtttcc cggccaagga atttcatgag gctggctacc atgtagtatt
     2881 cagcggcgga agccgttaca acggcgttgc cgtggcttct ctcgaggagc cacgggatgt
     2941 ctgcttcggt tttgaagacg ggggaccgcc ggatagagac aggttgatcc gtggtgtttt
     3001 ctcggggata ccgattttga acgcttacgt accccagggg acgagcaggg aggacccgcg
     3061 gtttcagtac aaactcgagt ggttccgccg cctgctcgcg ctgctcaaca ggttttactc
     3121 cagcaaacag cccttggtct gctgcgggga cctcaacgtg gcgcgggagg agatagacgt
     3181 ccacaaccct aaaagactcc ttggacacgt ggactttaat cccgaggtat gggaggcctt
     3241 cgatagcctg aaggcctggg ggtttgtaga cgtctttagg aaatttcatg ccggagagcc
     3301 cggtcactat acgttttttg attaccgcgt tccccggtcg gtggagaggg gcctgggttg
     3361 gagggtcgat catattctgg ccacaccgat tatggcggag aaggcagtga atgcttatat
     3421 cgacatggag gcgaggcggg cggaaaagcc ttctgaccac accgtgctag tggcagaatt
     3481 caagacatga aaaatcgagg agcttgggaa attcattgct gggtatttct tgggccggtt
     3541 gagaaatgat gggctcgcaa aaagtcgaga attactactt tcgtcattcc ggcgaaag
//
LOCUS       DAOTMX010000170         3580 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_20669_length_3580_cov_3.938440, whole genome shotgun sequence.
ACCESSION   DAOTMX010000170 DAOTMX010000000
VERSION     DAOTMX010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3580
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20669_length_3580_cov_3.938440"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..745)
                     /locus_tag="P5573_05690"
     CDS             complement(<1..745)
                     /locus_tag="P5573_05690"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HRT27587.1"
                     /translation="MDGWRFIPYHKFGAFENMAIDEAIFRGNQLGQSPPTLRFYGWDP
                     PALSVGYFQDVDQEVDVGYCRKNRVDIVRRPTGGKAVFHDRELTYSFISSEKQGPFTP
                     GVLGTYLAISRCILKGIAGLGIAAEVAQDSPSVERVNTAHCFSFPSRYEILIAGKKVC
                     GSAQTRANGAFLQHGSLLLCFDPAKALCAIRDRRGRVTEDTLREAVTYLGAYLPEGQT
                     DPEYLSGVLKAGFEDILRVEFVEGRLTPEE"
     gene            complement(751..1149)
                     /gene="gcvH"
                     /locus_tag="P5573_05695"
     CDS             complement(751..1149)
                     /gene="gcvH"
                     /locus_tag="P5573_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HRT27588.1"
                     /translation="MMVFPDDLLYSREHTWARVDGNLVTVGLTDYGQAKLGEILNIDL
                     PEVDTYVEQDEPFGTIESAATVTELISPVSGDVVRVNDDIADDVGIINSDPYDTGWMI
                     VVEMTDASELDNLLDSSEYNDYVLGEEGLD"
     gene            complement(1200..2294)
                     /locus_tag="P5573_05700"
     CDS             complement(1200..2294)
                     /locus_tag="P5573_05700"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HRT27589.1"
                     /translation="MVIKRDFKGARGRIERVRGTAATLALEAILFLDMANIRYLVGFT
                     GSEGALIVGEKNNYLLVDGRYTSQARREAHDAEVREYRDKVEGIASIIEGEGWKNCGI
                     ESMAVTLRDYIKLRDRLGGRELRCLAEEVAFLRAVKDAAEVDKVKEAIGISSSAFLSV
                     LAGLRPGVEERDVALELEYQIRRGGAENVSFETIVASGENSALPHARPGRRRLEAGDL
                     VVIDYGAVCEGYCSDETCTVAVGDVSDRQREVYGIVKEAHDRAIAAVRAGVACSEVDR
                     VARDFLTERGLGKHFSHGTGHGVGLRVHEEPRLSYLSKDRLEPGMILTVEPGVYLPGS
                     WGIRIEDMVIVEEEGCKVLTTIPKDLRVVG"
     gene            complement(2272..2982)
                     /locus_tag="P5573_05705"
     CDS             complement(2272..2982)
                     /locus_tag="P5573_05705"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRT27590.1"
                     /translation="MDISDRRIDENRLSARFSVEKDEFFLRAEDYLQRSQFQEALLLA
                     KERARLHPGDFDALLVSGIALLKSGREEEAAAAFEEIREGILRLSVALEYLGDIYAGR
                     GETEQARGCYRSLAGIDDDPELVERVLRKLDSLDEMDVVSDDAAAGEIPADFETLTVA
                     ELFLKQGYLEQAAKVLAALSLREPDNEKVRKLLEEVSARLAEKEAPLRPEAAVIAELD
                     RWLHRLLKLRQDGYQERL"
     gene            3218..3574
                     /gene="queF"
                     /locus_tag="P5573_05710"
     CDS             3218..3574
                     /gene="queF"
                     /locus_tag="P5573_05710"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR03139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HRT27591.1"
                     /translation="MTTSTHMDTIENKYKDRDYEVNIRFPEFTCLCPMTSQPDFAEIK
                     ISYVPGERLVELKSLKFYLNGFRNRGIFHEEAVNLIRDDFVETVRPKRLEVVGNFNVR
                     GGIYTTVRASYADSGS"
BASE COUNT          801 a         1143 c          862 g          774 t
ORIGIN      
        1 cctcctccgg ggtcaaccgg ccctccacaa attctacccg cagaatatct tcaaagcccg
       61 ccttcaacac cccggaaaga tattcagggt ccgtctgacc ttcaggaaga taagccccca
      121 ggtacgtaac ggcctctcgc aaagtgtctt ccgtgacacg cccacgtcgg tccctgatag
      181 cacatagggc cttcgccggg tcaaaacaca gaagcagcga cccgtgttgc aagaaggccc
      241 cgttcgctcg tgtctgggcg ctcccgcaga ccttcttccc tgctatgagg atttcgtacc
      301 gggagggaaa agaaaagcaa tgggccgtat taacacgctc cacagatgga ctgtcctgcg
      361 ccacttccgc agcaatgccc aagccggcaa tccccttgag aatacacctg cttatcgcca
      421 ggtacgtccc gaggacgccc ggagtgaacg gtccctgttt ttcactcgag atgaaggaat
      481 aggtcagctc tcggtcatga aaaacggcct ttccacccgt gggccgccgc acgatgtcga
      541 cccggttctt tctacaatat cctacgtcta cttcctgatc gacgtcctga aaatacccaa
      601 cggaaagcgc gggcgggtcc cacccgtaga atctcaatgt cggaggcgac tgccccagtt
      661 ggttgcccct gaagatggcc tcgtcgatag ccatgttctc gaaggcgccg aacttatggt
      721 aggggataaa cctccatcca tccacgaaag tcagtccaac ccttcctcgc cgaggacgta
      781 atcgttgtat tccgaagaat cgaggagatt gtccagctcg ctcgcatccg tcatctccac
      841 gacgatcatc caaccggtat cgtaggggtc gctgtttatg atccccacgt catcggcgat
      901 gtcatcgttc accctcacga catccccact cacaggggag atcagttcgg tcactgtcgc
      961 agccgactcg atggttccaa agggttcgtc ctgctccacg taagtgtcga cctccgggag
     1021 gtcgatgttg agtatttcgc cgagctttgc ctgcccatag tccgttaaac cgaccgtaac
     1081 caaattccca tccactctcg cccaggtatg ctcgcggctg tagagcagat catcaggaaa
     1141 aaccatcata cgaaacaacc ctttcctact tttatctctg tatcgtatat ttccttgcct
     1201 caaccgacga cacgcaaatc cttgggaatc gtcgtgagga ccttacatcc ttcttcctca
     1261 acaatcacca tgtcttcgat acggattccc cacgaaccgg gcaaatagac accgggctca
     1321 acggtcagga tcatgcccgg ttccaggcgg tccttcgaca ggtatgagag cctcggctcc
     1381 tcgtggacac gcaagccgac tccgtgtcct gtcccgtggg agaaatgctt tccgagaccc
     1441 ctttccgtca gaaaatccct ggctacccgg tctacttcac tgcaggccac gccggcccta
     1501 acggccgcta tagctctatc gtgggcttcc tttacaatcc cgtagacttc ccgctgcctg
     1561 tccgacacat cgccgaccgc cacagtgcac gtttcgtcgg agcaataacc ctcgcagact
     1621 gcaccgtagt cgatcacgac caagtcgcca gcttccaatc ggcgtctccc cggcctggcg
     1681 tgcggcagcg cggaattttc tcccgaggcg acgatcgttt cgaacgagac gttctccgcg
     1741 ccgccccgcc gaatctgata ttcgagctcc aaagccacat ctctctcttc gaccccgggt
     1801 ctcaagcccg ccagcacgga gagaaacgca ctggaagata tccctatcgc ctccttgact
     1861 ttgtcaacct cggcagcgtc ttttacggct cggaggaagg cgacttcctc cgcaagacat
     1921 ctcaattcac ggccgcccag acggtcgcgc agcttaatgt agtcacgcaa ggtcaccgcc
     1981 attgattcga tgccgcaatt tttccagcct tcgccctcga ttatcgaagc gatgccctcg
     2041 accttgtctc tgtattcgcg aacctcggcg tcgtgagcct cccggcgggc ttgactcgta
     2101 taacgaccgt caaccagaag gtagttgttc ttttcgccga cgatgagtgc cccttcgctg
     2161 cccgtgaatc ccacgagata gcggatattc gccatgtcca agaacagaat cgcctccaac
     2221 gcaagcgtgg cggccgtacc gcggacccgt tcgatccgac cccgggcacc tttaaagtct
     2281 ctcttgataa ccatcttgtc tcaacttcag caggcgatgg agccaccgat ccaattctgc
     2341 gatgacagcg gcttcaggcc ggagaggcgc ctccttttcc gccagccgag cacttacttc
     2401 ttcgagtaat ttccggacct tttcattgtc gggctccctg agggagaggg ccgcaagaac
     2461 cttcgccgcc tgctccaggt acccctgttt cagaaaaagc tccgccactg tcagggtctc
     2521 gaagtcggca gggatttccc cggcagccgc gtcatccgac accacatcca tctcatcgag
     2581 ggaatccagc ttccttaaaa ctctttcgac cagctccggg tcatcatcga tgccagccag
     2641 ggatcgataa cagccccgcg cctgctccgt ttctccccta cccgcgtaaa tgtcacccag
     2701 gtactcaagg gcaaccgaca gtcgaagaat gccctccctt atttcttcaa aggccgctgc
     2761 tgcttcttct tctctccccg atttcagcag ggcgatccct gagaccagca gggcatcaaa
     2821 gtctcctggg tgcaggcggg ccctctcttt cgccaagagc aacgcttctt ggaactgact
     2881 gcgctgcaaa tagtcctcag cgcgaagaaa gaattcatct ttttcaacgg agaaccgagc
     2941 agacaaccgg ttttcgtcta ttcgcctgtc cgaaatgtcc acgaaaaaaa cctttgtaca
     3001 acggagggac gaacgctaac acagcaccga agaacctgtc aagcggtacg attttccccg
     3061 ccgccgcgac ctaacatcgg acaataccac gtcatttccc ccgaagcggg aaatgattgc
     3121 acgcctcgct tcataatggt attaatcgca aataccggta gctacttaga ccgacgatga
     3181 aaggaccttg cgttaaagtg gccgggattt taagagaatg acaacgagta ctcatatgga
     3241 caccatcgag aacaagtata aggaccgcga ttacgaggta aacatccggt tcccggaatt
     3301 tacctgcctt tgccccatga cgtctcaacc cgatttcgcg gagataaaaa taagctacgt
     3361 cccgggcgag cggctggttg aactgaagtc gctgaagttc tacctgaacg gcttccgaaa
     3421 ccgggggatc ttccatgaag aggcggtcaa tcttatccgg gacgacttcg tcgaaacggt
     3481 gcgccccaaa aggctggaag ttgtcgggaa tttcaacgtc aggggtggga tatacacgac
     3541 cgtaagggca tcctacgccg attccggttc gtgacgcccg
//
LOCUS       DAOTMX010000171         3556 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_20828_length_3556_cov_3.352471, whole genome shotgun sequence.
ACCESSION   DAOTMX010000171 DAOTMX010000000
VERSION     DAOTMX010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3556
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20828_length_3556_cov_3.352471"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="P5573_05715"
     CDS             complement(<1..479)
                     /locus_tag="P5573_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HRT27592.1"
                     /translation="MAMTITEKILALHAGLENVWPGDMIQSKVDLAFAHDVTGPIAVK
                     MFRETGANGVFDREKVVLVADHFVPAKDIASAGQAKVIREFAREQGLENFFESGDAGI
                     GHVLLPEKGFIVPGQVVIGADSHTCTYGALGAFSTGVGSTDLAAVMATGETWLKVPPT
                     "
     gene            complement(673..2289)
                     /gene="cimA"
                     /locus_tag="P5573_05720"
     CDS             complement(673..2289)
                     /gene="cimA"
                     /locus_tag="P5573_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HRT27593.1"
                     /translation="MKEWDVVIYDTTLRDGAQGEQVSFSAEEKIRIARRLDEFGVHYI
                     EGGWPGSNPKDVRFFEMARQIKFKNTRPAAFGSTRRAHTTAGECPNVRALVEAETPAV
                     TIFGKSWDLHVKEVLRVSLKENLAMIRETVEFLKSRGKEVIYDAEHFFDGYRHNRKYA
                     LQTLQAALDGGADMIVLCDTNGGTLPSDVSRVVGDVTRVVSPERMGIHAHNDGGLAVA
                     NSIAAVEEGVKMVQGTINGYGERCGNADLIQIIANLQLKMNKRCIPEESMKQLTHLSL
                     YVSEIANVPPLNSRPFVGRSAFAHKGGIHVHAVLKNPLAYEHIEPEWVGNQRRVLVSD
                     YSGKSSIEYKAKELGLSLDEDDAKSRKVVEKLKELEHEGYTFEAADGSLSLLIKKTTG
                     EFEAPFTLECYRAIDEKTGDKPSVSQALIKVSVGTEEEITAAEGNGPINALDNALRKA
                     LTKFYPHLSEMHLTDFKVNIIEGSEGTASKVRVFIESRDEEDVWSTIGVSESVIEASW
                     LALVDSIEYKLSKKPVPNLKFKVEKVVKKN"
     gene            complement(2585..2848)
                     /locus_tag="P5573_05725"
     CDS             complement(2585..2848)
                     /locus_tag="P5573_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27594.1"
                     /translation="MKAFRMNKYIICAELLEEAKEFFVREIGEALPADIDELSWDCEV
                     CSASGETRTLRDIINGVMDERSAWLRMGIPCDLLWPFIAAKTE"
     gene            complement(2858..>3556)
                     /locus_tag="P5573_05730"
     CDS             complement(2858..>3556)
                     /locus_tag="P5573_05730"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HRT27595.1"
                     /translation="RARIPSKPHKPVKAKPLPRMGYTIQAGAFSSEVNAAKFVRSLKQ
                     DGVDAYYFVHRSGLYKVRFGNFPTEKQARARALSLQESGVIDEFFIVKPEDYAAAQVA
                     QRGGSYLRKEIVRTAESFVGVSYLWGGSSADEGFDCSGLTMAVYRYNGLDLPRSSKEQ
                     YESGTPVDKDNLRQGDLVFFTTSGVKKVSHVGVYVGDGKFIHAPARGKKICLASLSQR
                     YFADRFVGGRSYIQ"
BASE COUNT          735 a          995 c          959 g          867 t
ORIGIN      
        1 gtgggtggca ctttcagcca tgtttcccca gttgccatga cggcggcgag gtcggtgctg
       61 cccactcccg tcgagaaagc acccagcgcg ccgtaggtgc aggtatggct gtcggcgccg
      121 atgacgacct gtccgggaac gatgaagccc ttttcaggta agaggacatg accgatccct
      181 gcgtcaccgg actcaaaaaa gttttcgagt ccctgctcgc gggcgaattc cctgataacc
      241 ttggcctgtc ccgcagaggc gatgtctttt gcgggcacaa aatggtcggc gacgaggacg
      301 actttctccc ggtcgaatac cccgtttgct cccgtctcgc ggaacatctt gacggcgatc
      361 ggacccgtca cgtcatgggc gaaagccagg tccacttttg actggatcat atcgcccggc
      421 catacgttct ccagccccgc gtgcagggcc aggatttttt ccgttatggt catggccatg
      481 ataaacccca gagagttcaa agtagacctt tgaagaacgg cgcctcatgt cattccgacg
      541 aaagtcggaa tccagaaccg cttgaaaaca ctggatcccg tgtcaaacac ggaatgacgg
      601 aaaggcgatt taattacaaa ttcaaaggtc tccaaaggtc gagattactt ttccgtcccg
      661 agagctcttt ttttaattct ttttcaccac tttttcaact ttgaacttaa gattaggaac
      721 gggttttttg ctcagcttgt actcgatact atcgacgagg gcgagccagc tcgcctcgat
      781 gacgctttcg gaaacgccga tcgtgctcca gacatcctcc tcgtctctgg actcgatgaa
      841 aacccgcacc ttggaggccg taccctcgct cccttctatg atgttgacct tgaagtccgt
      901 caggtgcatt tcgctcagat ggggatagaa cttcgtcagg gctttccgta gggcgttgtc
      961 gagcgcattg atggggccgt taccctcggc ggccgtgatc tcctcttcag tccctacgga
     1021 gaccttgatc agcgcctggg agaccgacgg cttgtccccc gtcttctcgt cgatggcacg
     1081 atagcactcc agggtgaaag gcgcctcgaa ttcaccggtc gtcttcttga tcagaagtga
     1141 gagagacccg tcggcggcct cgaaggtata gccttcatgt tcgagttcct ttagtttttc
     1201 gacgaccttc ctgctttttg cgtcgtcctc atcaagagaa agcccgagct ccttcgcctt
     1261 gtattcgatg ctgctcttcc ccgaatagtc ggagaccagg acgcggcgtt gatttcctac
     1321 ccattcgggc tcgatatgct cgtaggccag gggattcttc aggacagcgt gaacatggat
     1381 gcccccttta tgggcgaagg cgcttcttcc gacgaagggc ctggaattga gcggtgggac
     1441 gtttgcgatc tcgctcacat aaagagagag atgtgtcagt tgtttcatgg attcttcggg
     1501 tatgcaccgc ttgttcatct tgagctggag gttggcgatg atctggatga ggtcggcgtt
     1561 accgcagcgc tcgccgtatc cgttgatggt tccctggacc atcttgacgc cctcttcgac
     1621 ggcggcgatc gaattggcca ctgcaagtcc gccgtcattg tgggcatgga ttcccatacg
     1681 ttcgggagaa acgacccgtg tgacgtcgcc gacgacccgg gaaacgtcag acggcagtgt
     1741 cccgccgttg gtgtcgcaca gaacgatcat gtcggcgcct ccgtcgagag cggcctggag
     1801 tgtctggagg gcgtattttc tgttgtgcct gtaaccgtcg aaaaagtgct cggcgtcgta
     1861 tatgacttct ttacccctgg atttgaggaa ttcgacggtc tcgcggatca ttgcaaggtt
     1921 ttctttgagc gataccctca gcacttcctt tacgtgaagg tcccagctct tgccgaagat
     1981 ggtaacggcg ggcgtttcgg cttcgaccag ggcccggaca ttcgggcact caccggctgt
     2041 tgtgtgcgcc cgtcttgtgc ttccgaaggc cgccggacgg gtgtttttga atttaatctg
     2101 ccgtgccatc tcgaagaacc gtacatcctt gggattggaa ccgggccagc cgccttcgat
     2161 ataatggacg ccgaattcat ccagccgcct cgcgatacga atcttttctt ctgcggagaa
     2221 gctgacctgc tccccctgtg ccccgtccct caatgtggtg tcataaataa cgacatccca
     2281 ttctttcatg atagacctct tagttccgag ttctgagcaa tcgatcgtcc ccggtggtag
     2341 accgccatgt ccgggcacgc cttaacctat ctgccgcaat aaaaaatccg ttatcaggtc
     2401 gacctgataa cggattatga agtctctctt gtgattacat cagtccatca tcaggtctcg
     2461 atgggcaccc tgaattatga tgccgataat gatgatggcg atgtctctca acacagattg
     2521 cacgccccaa cttccaaagt ggaaaggact ataaaacttg gagtttcttt tgtcaataaa
     2581 aaaatcattc ggtctttgca gcgatgaaag gccaaaggag gtcacagggg atgcccatgc
     2641 gcagccaggc actgcgctcg tccatcactc catttatgat gtcccgtaag gtacgggtct
     2701 ctccgcttgc actgcagacc tcgcaatccc atgacaactc atcgatatcc gcgggaagag
     2761 cctcccctat ctcacggaca aagaactcct tcgcctcttc aagtaattcg gcgcaaatga
     2821 tgtacttgtt cattctaaag gctttcatgg tcggctccta ctggatgtat gatcggcctc
     2881 cgacaaatct atcggcaaaa tagcgctgcg atagggaggc aaggcatatc ttctttcccc
     2941 gtgctggggc gtggatgaac ttaccgtctc ccacgtagac tccgacgtgg gagaccttct
     3001 tgacgcccga ggtggtgaag aaaacaaggt caccttggcg caagttgtct ttgtcgacgg
     3061 gtgtgcccga ttcatactgc tctttggacg accggggcaa gtcgagcccg ttgtaacggt
     3121 aaaccgccat ggtcaggcca ctgcagtcga acccttcgtc tgcggaagaa ccgccccaga
     3181 ggtatgaaac gccgacaaag ctttctgccg tcctgacgat ctccttccgg agataggaac
     3241 cgccgcgctg agcaacttgt gcggcggcgt agtcttccgg cttgacgatg aagaactcgt
     3301 cgatgacccc cgactcctgt aaggatagcg cccgcgctcg agcctgtttt tccgtcggga
     3361 aattaccgaa acgcaccttg tagagcccgg accggtggac gaagtagtaa gcgtcgacgc
     3421 cgtcttgctt cagggagcga acgaatttag ccgcattcac ctccgaagag aaagcgccgg
     3481 cctgaatggt gtaacccatc ctcgggaggg gtttcgcctt cacgggtttg tgaggttttg
     3541 agggaatgcg ggctcg
//
LOCUS       DAOTMX010000172         3552 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_20855_length_3552_cov_4.069774, whole genome shotgun sequence.
ACCESSION   DAOTMX010000172 DAOTMX010000000
VERSION     DAOTMX010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20855_length_3552_cov_4.069774"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            286..1137
                     /locus_tag="P5573_05735"
     CDS             286..1137
                     /locus_tag="P5573_05735"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRT27596.1"
                     /translation="MLGDMFTEGRLVKVIDFHVHIGTKDQFVPWVVEYFATNTHYLEN
                     FAEKITPEGVIEFFKSEGVDRLVILAEYAPEASAMITNEFVSEFSRGHEELIPFGGLN
                     FLDETPYYEQARHAVEVLGVKGFKLLPSYQHFYPNDRALYPFYEYVQSLGMPVMFHTG
                     TSVFHGTRIKYADPLLLDEVADDFPKLNITMEHGGRTFWYDRAAWLVSKYRNMHIGIA
                     GIAIKHLPKYFPRLEQYSDRFIFGSDWPSLPSLKGLIDRVYALPYGKETKEMILCKNA
                     QRLLYGV"
     gene            1450..2628
                     /locus_tag="P5573_05740"
     CDS             1450..2628
                     /locus_tag="P5573_05740"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRT27597.1"
                     /translation="MELWRKNLYGLWVAQFLSTAGLNMVIPFLPMFLRELGVTGDASI
                     RLWSGLVFAAPFLVSAVMQPFWGVLGDRVGRKPMVLRAMLSLSLANFLMGFSQSAVQL
                     LLFRFFQGCLSGFLAPSLALMTSCAPPEKTGYALGTLQTSLITGLIAGPLLGGVLAHF
                     MGSRLVFFWTGFFCLCGALIVFRFVLEEFDSGRKEQKSGLRQNLGSVFNSVELRKMFF
                     VLILIQFAINFIVPFLTLYVEFLKVPQDYLSLVSGAVFGVTGITSAVTAPLWGRKADE
                     TGYYRVLRFAIVGMVVFMLPQAFVANAVQLLLLRAGLGIFVSGTIPVIYSIIRQSTRE
                     EARGGIYGIFQSGILLGNMIGPLAGGGLAALLGLRNVFLASGFILCFALLSGIVQDKK
                     "
     gene            complement(2763..>3552)
                     /locus_tag="P5573_05745"
     CDS             complement(2763..>3552)
                     /locus_tag="P5573_05745"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HRT27598.1"
                     /translation="ITACSREIKNGERVLIGVGLPLLGAMLAKRTHAPDCKMAYETGS
                     FDSSPSRTPFSVVDADLVPRAVHAGQLTDIMALYLQRGFIDVGFIGGAQVDKFGNLNS
                     TCLGDYFEPNVRLPGSGGAHDFGVFARRSLIVMIHEKRRFVEKCDYVTTPGYLNGGNS
                     RYEAGLPEGTGPAAVISTLGVFRFSPDKKEMYLETYHPGVTVDSIKENVNWDLKISPI
                     VRETEPPTEEQIRIIRELDPDGFFLRRGEYNKRIRAQAENMGWI"
BASE COUNT          780 a          920 c          921 g          931 t
ORIGIN      
        1 ctcgcggagg ttttccgccc gccgccgcga gtcctccagc gtcgaaccgg ggagagctat
       61 ctgggacgcc ctttacatat tcacgctgaa ggcaggcccc gtgcccatcg tcaccttcat
      121 gctacggcta ttgacaggtt acgccatatc cgtctcaatc ccctgaggac aggcctgtcc
      181 ctgacctttc agagagaagg acctgactga cgagcagggg tggcacttga cgagtttcca
      241 aatataaaaa agatgtaaaa gcatcccctt acccatgatg aaatattgct aggggacatg
      301 tttacggagg gaaggctcgt gaaagtcatc gatttccatg tacacatagg gacaaaagat
      361 cagtttgtgc cgtgggtagt ggagtacttt gcaacgaaca cccattacct ggagaacttc
      421 gccgagaaaa ttacgcccga aggggtaatt gagtttttta aatcagaggg tgtggacagg
      481 ttggttattc tcgccgaata cgcacccgag gcatcggcca tgataactaa tgaattcgtt
      541 tccgaattca gtagaggcca tgaagagctg atccctttcg gcgggctcaa cttcttggat
      601 gagacgccgt attacgagca ggccagacat gccgtggaag tacttggcgt gaaaggcttc
      661 aagcttctcc cctcttatca gcacttctac ccgaatgatc gggcactgta tcccttttac
      721 gaatatgttc agtccctggg tatgcccgta atgttccata ccggcacgtc cgtttttcac
      781 ggtacccgca taaaatacgc ggacccactg ctgcttgacg aggttgccga tgattttccc
      841 aaactcaaca ttaccatgga gcacggcgga aggacgttct ggtatgacag ggctgcctgg
      901 ctcgtttcga aatacaggaa catgcatatc ggcatagccg gaattgccat aaagcatctg
      961 cctaaatatt ttccccgact tgagcaatac agtgaccgct tcatcttcgg cagcgactgg
     1021 ccgtcgttac cgtctctcaa aggtctgata gacagagtct atgcgcttcc ttacggaaag
     1081 gagacaaaag aaatgatcct ctgcaaaaac gcgcagaggt tgctttatgg ggtatgaggt
     1141 tcctgttcgg atcattaccg tcccaaaaga aggaagaaac gagactgcgg agaaaggccc
     1201 gttgtgaaaa gcaacatacg gcaataaata acacagaatg actcacaaaa ttttcccatt
     1261 ctgtccattt gttaaaaact tgacactgtg gcagaagggc ccttgcaaga gcggtcagtc
     1321 cagtcgagag attgaccgct ttccttttgc cttccttgag gaaggtgggc ataaaaagaa
     1381 gctatgacta tagtcttata cccggggacc tctaaaacat cttgctgact gtcccctgac
     1441 gctttgtata tggaactctg gcgcaagaac ctttacggcc tgtgggttgc tcagttcctg
     1501 tcgaccgccg gtttgaacat ggttatacct tttttgccga tgttccttcg cgaactcggt
     1561 gtgaccggcg atgcatcgat caggctgtgg agcggcctgg tttttgccgc accgttcctg
     1621 gtctcagcgg tcatgcagcc cttttggggg gttctggggg atagggtcgg acggaaaccg
     1681 atggtcctga gggccatgct gagtctatcc ctcgccaatt ttctgatggg tttttctcag
     1741 agtgccgttc aattgctgtt gttccggttt tttcaggggt gtctctccgg ttttttggct
     1801 ccctctcttg cgttgatgac ttcctgcgcg ccccctgaga agacggggta tgctcttggg
     1861 acgctgcaga cctcgctgat taccggtttg atcgccggtc cgctccttgg tggagtgttg
     1921 gcccacttca tgggcagtcg gctggttttc ttctggaccg ggtttttctg cctttgcggt
     1981 gccctgatcg tcttccgctt cgtcctcgag gaattcgatt cagggagaaa ggagcagaaa
     2041 tcggggttac gccagaacct gggatccgta ttcaattcgg tggagctcag aaaaatgttc
     2101 tttgttctga tcctcatcca gtttgcgata aacttcatcg ttccgttcct cacgctttat
     2161 gtggaattcc tgaaagtacc gcaggactac ttgagtctcg tcagtggagc cgtcttcggc
     2221 gttacgggca taacaagcgc cgtgaccgct cctctctggg gaagaaaggc cgacgagaca
     2281 ggatattaca gggtcctccg cttcgccatc gtcggtatgg tcgtctttat gctgccccag
     2341 gcctttgtcg caaacgccgt gcagttgctc ctgttgcgtg ccggcctcgg tatctttgta
     2401 tcgggaacga tacccgtaat ctattccatc attcgtcaat ccacccgcga ggaggcgcgg
     2461 ggcggcatct acggtatctt tcaaagcggg attctgctgg ggaacatgat cggacccctg
     2521 gccgggggcg ggctcgccgc cttacttggg ttgaggaacg tctttttggc ttccgggttt
     2581 attctttgct ttgcccttct gtctgggata gtgcaagata aaaaatgaaa ggaaaatgcc
     2641 gtcgggcttg gtgcacgttc gtttcggcat ttgccacacc tgccaaaatt aagataacac
     2701 cttatccgtc ggggtaaaag atattggggc ctgatcgtgt gatcaggccc ttgttatcta
     2761 ttttaaatcc atcccatgtt ttcagcctgc gcccttattc ttttgttgta ctcgccgcgt
     2821 ctcaggaaaa acccatctgg atccagttcc ctgataatcc tgatctgctc ttccgttggc
     2881 ggttcggttt ctcggactat cggcgaaatc ttgaggtccc agttaacgtt ttccttgatg
     2941 gaatctaccg tgacgccggg atgataggtc tcaaggtaca tctctttttt atccggactg
     3001 aaccgaaata cgcccagcgt ggatatgact gcggccggcc cggttccttc cgggagtccc
     3061 gcctcataac gactgtttcc gccgtttagg tatccaggtg tcgtcacgta atcgcatttt
     3121 tcgacaaaac gacgcttctc atgaatcata acgatcaaac ttcttctggc gaagacgccg
     3181 aagtcatgag caccgccgct tcctggaaga cggacgttcg gctcgaaata atctccaagg
     3241 caagtagaat taaggttacc gaatttatca acctgtgccc cgccgatgaa gccgacgtcg
     3301 ataaagcctc gctggaggta aagagccatg atgtccgtca actgtcccgc gtgcacggcc
     3361 ctcggaacta aatcggcgtc gacgacacta aaaggcgttc tcgatggtga agaatcgaag
     3421 gaacctgttt catacgccat cttgcagtca ggtgcatgtg tccgctttgc cagcatggcg
     3481 ccgagaagag gtaaacccac gcctataagt acccgttcgc catttttaat ttcccgtgaa
     3541 caggctgtaa tc
//
LOCUS       DAOTMX010000173         3546 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_20890_length_3546_cov_3.145517, whole genome shotgun sequence.
ACCESSION   DAOTMX010000173 DAOTMX010000000
VERSION     DAOTMX010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20890_length_3546_cov_3.145517"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /gene="cmr1"
                     /locus_tag="P5573_05750"
     CDS             complement(<1..438)
                     /gene="cmr1"
                     /locus_tag="P5573_05750"
                     /inference="COORDINATES: protein motif:HMM:TIGR01894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module RAMP protein Cmr1"
                     /protein_id="HRT27599.1"
                     /translation="MIKKEVVMFYVSRFNGIEKRQYECEVVTPMFLGGADPRGAELRV
                     PPIKGAMRFWWRATCGIESIKEMAEEEAKIFGSTEKKSKVSLWIENIDAEPKLEKLPE
                     GTKVPVEGKTYSASIVHYLAYGFYERLDCTPGVRQFWLGQSDRG"
     gene            complement(664..1092)
                     /locus_tag="P5573_05755"
     CDS             complement(664..1092)
                     /locus_tag="P5573_05755"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRT27600.1"
                     /translation="MIWVIDASVAVSWFLEEDNHSHADAVLRRLIDRPNSFAVPELFC
                     FEVFSVLCRIHPRGIDAFTKGVIPLITGGVYRRPMTESLANRAIVYIGKGLTGYDACY
                     AALASELKGKWLTFDRAAHESIKKERISHDLTEGLPDGWM"
     gene            complement(1089..1361)
                     /locus_tag="P5573_05760"
     CDS             complement(1089..1361)
                     /locus_tag="P5573_05760"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HRT27601.1"
                     /translation="MKEVNALKLRNNLGEILEQLTKTGEPVLISKGKKAKAVLITVEQ
                     FEKRFLDYQAEEKKKELLDTIQGLKERRTGSKSSLEVLRELRGYES"
     gene            complement(1437..2561)
                     /gene="csm6"
                     /locus_tag="P5573_05765"
     CDS             complement(1437..2561)
                     /gene="csm6"
                     /locus_tag="P5573_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated ring nuclease Csm6"
                     /protein_id="HRT27602.1"
                     /translation="MKNILLAVVGLSPQVITETLYALHQSGREVNAIHVITTRQGKEK
                     INAHLLSPRDGRYYQYLREYGIDRRSVDFGFDNVHTVKDPHGIEIDDITGEEENEWLL
                     RCCLEWAFRLTRDPNNAVFFSVAGGRKTMSACLMVAAQFYGRPQDRVFHVLVTPEFES
                     NRDFYYPPKTSIPVELRDRNGQVFIKESKYAAVNLVPLPFVSVRDQLSGDMLRRPKDP
                     ATLLLSLVREEPHQLTVDLTASRLVYKKRELDMMPARLALYAWFALHKKECKKETPTC
                     RGCSDCFQSVYDVLGGHERISELYRNLSRNRDFYAMRDKGILNLGKEDFNTYRSKIQR
                     DIEQGFGLEARREIAISSVGARPNKRYGITIDREKIKVVL"
     gene            complement(2561..3529)
                     /gene="cas6"
                     /locus_tag="P5573_05770"
     CDS             complement(2561..3529)
                     /gene="cas6"
                     /locus_tag="P5573_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR system precrRNA processing
                     endoribonuclease RAMP protein Cas6"
                     /protein_id="HRT27603.1"
                     /translation="MLYGRYVFSCRFLDEAELPEYKGSTFRGVFGHALKRTVCALKRQ
                     GCEGCLLHPKCIYVFLFETPFPVEANGKRRLSSPPHPYVIEPPETERTRFQPGDSFEF
                     ALLLFGMANEYLPYFIYAFEQIGGLGIGKRVNGRAARFKLENVSSGGEPVYSSNDATL
                     RAGSFTEELTEETLREGVPADEIELRLVTPLRVKFGNSLRAELPFHVLVRAMLRRTSS
                     LFEYYGAGEPSLDYRGLVDRAKKVAIEESAVEWFDWKRYSNRQDQSMLMGGITGSISY
                     AGNLSEFIPFIRFCEKVHIGKQTTFGLGKINLVSLSIGEEGRQEGK"
BASE COUNT          727 a          989 c          951 g          879 t
ORIGIN      
        1 acccctgtcc gactgcccta accaaaactg tctaactcca ggggtgcagt ccagtctttc
       61 ataaaatcca taagcgagat aatgaacgat cgacgcgcta taggttttac cctctacagg
      121 aaccttcgta ccttcgggaa gtttttcaag ctttggctca gcatcgatat tttcgatcca
      181 tagggagact ttcgatttct tctctgtcga accgaagatc ttcgcctcct cttcggccat
      241 ctctttaatg ctttctatcc cacatgttgc ccgccaccag aagcgcattg cccctttgat
      301 aggggggaca cgaagttctg caccccgagg gtctgccccc ccaaggaaca tcggtgttac
      361 gacttcgcat tcatactgtc gcttttctat cccgttgaat cgagagacat agaacatcac
      421 gacctccttt ttgatcactt gcatcctttc agccagctca gtgaataccc ataatgccgg
      481 aacgcccgca attcactgaa ctatgcggaa tggtcctcta tcgaaagcaa cgaaccgcat
      541 tcctcacggt tttacctccg ggcgatgtct cccatcacct cagtggccgg attatagggt
      601 gtatcttcga ctcgttccac ctctgcaacg ttgccgctgt aacccgccgg tagggacgct
      661 ttttcacatc catccgtcgg gaaggccttc tgtcaggtcg tgggaaattc gttccttctt
      721 gatcgattcg tgtgccgcac ggtcgaatgt gagccacttt cccttcaact cagacgccag
      781 ggcggcataa caggcgtcgt accctgttaa tccctttccg atgtacacga tggcccggtt
      841 cgccaggctt tccgtcatcg gccggcggta aacgccgccc gtgatgaggg gtatcacgcc
      901 tttggtaaag gcatctatgc cccgtggatg aatacgacag agcacggaga aaacttcgaa
      961 gcagaataat tccgggacag caaaggaatt cggccggtct atcaatcggc ggaggacggc
     1021 atctgcatga gaatgattat cttcctcaag aaaccacgac accgccaccg aagcatcgat
     1081 tacccatatc acgactcgta tcccctcagt tctctcaata cctcaaggct gcttttcgac
     1141 ccggtgcggc gttctttcaa cccctggatg gtgtccagca attccttctt tttctcctcc
     1201 gcctgataat cgagaaagcg cttttcaaat tgctcgaccg tgatgagaac ggctttcgcc
     1261 tttttcccct tgctgatcag gacaggctcg cctgtcttcg tgagctgttc caggatctca
     1321 cctagattat tcctcaattt gagggcattg acttctttca tcgcctttcc tctatgattt
     1381 ttgaaagaat ttagtatgat gcgctctaca cgtcaagtga atatattggt atacacttac
     1441 aggacgacct ttatcttctc ccggtcgatc gtgattccgt agcgcttgtt gggtctggcg
     1501 cccaccgacg aaatggcgat ttcccgccgg gcctctaggc cgaatccctg ctcgatgtct
     1561 ctctggattt tgctcctgta tgtgttgaag tcttccttgc ccaggttcag gatccccttg
     1621 tcacgcatgg cgtagaagtc tctgttacgg gagaggttgc ggtagagttc cgatattctc
     1681 tcgtgcccgc ccaggacatc gtagacgctc tggaaacagt cgctgcaacc cctgcatgtg
     1741 ggtgtctctt ttttacattc ttttttatgg agggcgaacc aggcgtaaag ggcaagcctg
     1801 gcgggcatca tgtcgagttc ccgttttttg tagacgagcc gcgacgccgt gaggtcgacg
     1861 gtgagctgat ggggttcttc ccggacgagg gagaggagga gtgtcgcggg gtctttgggg
     1921 cggcggagca tatcaccgga caactggtct ctgaccgaga cgaaggggag gggcaccagg
     1981 ttcaccgctg cgtattttga ttccttgatg aagacttgac cgttcctatc gcgcaactcg
     2041 acgggaatgg aggtcttcgg aggatagtaa aagtcccggt tactctcgaa ttccggcgtg
     2101 accaggacgt ggaagacccg atcctgggga cggccgtaga attgggcggc caccatcagg
     2161 caggcgctca tggtcttgcg gccgccggcg acggagaaaa agacggcgtt attcgggtcg
     2221 cgggtcagtc tgaatgccca ctccaggcag cacctgagga gccactcgtt ttcctcttcg
     2281 ccggtgatgt cgtcgatctc gatgccgtga gggtctttca ccgtgtggac gttatcgaag
     2341 ccgaaatcta cggagcgccg gtcgatgccg tattcgcgca gatactgata atacctgccg
     2401 tctctgggtg agagcagatg ggcgttgatt ttttccttgc cctggcgcgt ggttatgaca
     2461 tggatggcgt tcacctcgcg gcccgattgg tggagggcgt aaagcgtttc cgtgatgacc
     2521 tgcgggctga gtccgacgac ggctaagagg atgtttttca tcacttaccc tcctgacgcc
     2581 cttcttcgcc gatggagagg gataccaggt tgattttccc caggccgaag gtggtctgtt
     2641 tccctatgtg gaccttttcg caaaaacgga tgaaggggat gaattcgctg aggtttcccg
     2701 cgtagctgat gctgcccgtg attcccccca tcagcatgga ctggtcttga cggttggagt
     2761 acctcttcca gtcgaaccat tctacagcgg attcttcgat tgcgaccttt ttcgccctgt
     2821 cgacgaggcc ccggtagtca agggaaggtt caccggcgcc gtaatactca aaaagcgaag
     2881 atgtccgcct caacatggcc cttaccaaga cgtggaaagg gagttcggcc ctcaggctgt
     2941 tcccaaattt gacgcgaagg ggggtgacga ggcggagttc gatctcgtcc gccgggacgc
     3001 cttctctgag cgtctcctcc gtcagctcct ccgtgaacga accggcccgc agggtcgcat
     3061 cgttcgagga atagacgggc tcaccgcccg agctgacatt ttcgagtttg aaccttgccg
     3121 cccggccgtt gacccgtttt ccgataccga ggccgccgat ctgctcgaat gcgtagataa
     3181 agtagggcag gtattcgttc gccatcccga aaaggagaag ggcgaattcg aaggagtctc
     3241 ccggctgaaa ccgggttctt tcggtctccg gcggctcgat cacgtaagga tgaggagggg
     3301 aagaaagtcg tctttttccg ttggcttcca ccggaaaggg tgtctcgaag agaaagacgt
     3361 agatacattt cggatgcagg aggcaacctt cgcaaccctg gcgcttcagc gcgcagaccg
     3421 tccttttgag tgcatggccg aagacacccc ggaaggtcga ccctttatac tcgggaagct
     3481 cggcttcgtc cagaaatctg caggagaaga cataccgacc gtagagcatc cttacctcaa
     3541 cagact
//
LOCUS       DAOTMX010000174         3522 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_21051_length_3522_cov_3.574272, whole genome shotgun sequence.
ACCESSION   DAOTMX010000174 DAOTMX010000000
VERSION     DAOTMX010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21051_length_3522_cov_3.574272"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1040)
                     /locus_tag="P5573_05775"
     CDS             complement(<1..1040)
                     /locus_tag="P5573_05775"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRT27604.1"
                     /translation="MNFDEIFIKAIGNKPFPYQKKLAERADHLSLLEIPTGMGKTAAA
                     ILSWLWRKRFASEDIRDITPRRLVYCLPMRVLVEQTRNYAIEWLNNLDLLGGTANFDE
                     IDDKRILKEYDPWRGEDNPDKIRVHILLGGDTDRDWDIYPERDTIIIGTQDMLLSRAL
                     NRGYAMSRYRWPVQFGLLNNDCLWVMDEVQLMGNGLATTTQLQAFRRKLGTAKGVQSI
                     WMSATICSDWLETVDFELNSDAPGKLEITEEDKAQEVLQLRFRAIKKLQKADFKISDD
                     GKAEADLLVRLHKVGTLSLMIVNTVKRAQVIYSELKKKRLEAEVVLLHSRFRPPDRKA
                     ALERLLAPPNRTG"
     gene            complement(1259..2230)
                     /locus_tag="P5573_05780"
     CDS             complement(1259..2230)
                     /locus_tag="P5573_05780"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HRT27605.1"
                     /translation="MSSKVIYERFLWFHTKVRERKYPNTRSLAEKFEISRKTAQRDIA
                     FMRDRLGAPLYYVPERRGFAYDIDAYELPGFWLNETELSSLLVSYRLASSIPDNHLKN
                     SLKSFINKVISRFSGPGYVSIADLCEKVSVKNIEYSRTNEKIFQKTLEALLYGNTIRI
                     DYRSPHTGNVTSRDILPLHLLQYMGTWHIIAYCALKNEIRDFVLSRIRSIALSPLKIT
                     HRAAESSIKNYIRKNFGIIYSNETVDICLRFSPEVAGWVAEQVWHPKQKTQWEDENTL
                     CLIFPVADFREVKREILKFGSRVEVLSPERLREEIRQEIKKMGEIYR"
     gene            complement(2388..3164)
                     /locus_tag="P5573_05785"
     CDS             complement(2388..3164)
                     /locus_tag="P5573_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRT27606.1"
                     /translation="MYIDSHAHLEMKEFDRDRAKVVKRALEAGVEYIVTVGTTLEDCR
                     KALAIAKQHENVFAVVGIHPHEVKSIDPETYDTIRSLASADKVVAYGEIGLDFFRNLS
                     PKDVQIKRFGEQLELAEELDLPVVIHDREAHRETLEMLKGWKGKKRGVVHCFSGDRTM
                     AMECIDLGFYISIPGPVTFPKSEKMRDVVKGVPLNRLLIETDAPYLTPHPYRGKRNEP
                     AYVVFTARKIAEVKGITPEEVGKVTSQNAKDIFGLPIEIA"
     gene            complement(3195..>3522)
                     /locus_tag="P5573_05790"
     CDS             complement(3195..>3522)
                     /locus_tag="P5573_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRT27607.1"
                     /translation="VQAARAIGAGDMRIIFIHILPNAMASILVAATFGVAGAILTESA
                     LSFLGIGVQPPTPSWGNILTAGKDNIDIAWWLSFFPGLAILLTVLGYNLLGEGIRDSL
                     DPRLRG"
BASE COUNT          753 a          890 c          926 g          953 t
ORIGIN      
        1 cctgttctat tcggtggggc caatagtcgc tcaagtgcgg ctttccgatc cggcggccgg
       61 aatcttgagt gtaacaggac aacttccgct tccaacctct ttttcttgag ttctgagtat
      121 ataacctgtg ctcgcttcac ggtgttcact atcatcaatg acaatgtacc gactttatga
      181 agccgaacaa gcaaatctgc ctcagctttg ccgtcatccg aaattttgaa gtcagctttc
      241 tgcagctttt tgatcgccct gaaccgtaac tgtaaaactt cttgcgcctt gtcttcttcg
      301 gttatctcga gctttccagg tgcgtcactg ttcaattcaa aatccacggt ttccagccag
      361 tcagaacaga ttgttgcact catccagata gattgaacgc ccttggctgt gccgagcttc
      421 ctgcggaacg cctggagctg ggtggtcgtt gcgaggccgt tccccatgag ctgtacttca
      481 tccattaccc acagacagtc gttgttgagt aggccgaact ggaccggcca gcggtaacgg
      541 ctcatggcat agcctctatt gagggcgcgg gagagcagca tatcttgcgt tccaataata
      601 atggtgtcac gctcgggata gatgtcccaa tcgcggtcag tatcaccacc caggagaata
      661 tgcacccgta tcttatcggg attatcttcc cctctccatg ggtcgtattc cttgagtatg
      721 cgtttgtcgt ctatttcatc aaagttggcc gttcctccca gtaggtcaag attgttgagc
      781 cattcaatcg cgtaattcct ggtttgctca acgagcacac gcatgggtag gcagtagacg
      841 aggcgtctcg gagtgatatc gcggatatct tccgatgcaa accgcttacg ccacagccaa
      901 cttaatattg ccgccgccgt cttgcccatc cctgtgggaa tctcaagaag cgaaagatga
      961 tccgctcttt cagccaactt tttctggtaa ggaaatggtt tgtttcctat tgcctttatg
     1021 aatatttcat cgaaattcat ctctcttcac ctttacccaa gtaaactttc cggtgatttt
     1081 gttgcggctt tgggtctctt ccggttctcc cgataatctc aatgatagtt agtgggcatt
     1141 ttaaatggcg ccttgaggcg cctgaatgcg tgccgtcggc cccgctgatt caggatgtta
     1201 ctcgttcaaa tttacaatga cgttatcttc tatcacatcc ggtcccccaa atggtgtcct
     1261 accggtaaat ttcccccatt tttttgattt cttgtctaat ttcttctcgg agacgttcag
     1321 gagacaggac ttcaacccgg gagccgaatt ttagtatttc acgtttaact tctcggaaat
     1381 cggccacggg gaaaattaaa cagagggtat tttcatcctc ccattgcgtc ttctgcttag
     1441 ggtgccagac ctgctccgcc acccaaccgg ccacttcggg tgagaaacgc aaacatatat
     1501 cgacagtctc gttgctgtat ataatcccga agttttttct tatgtagttt ttgatcgagc
     1561 tttcggctgc gcgatgggtt attttaagcg gggaaagagc tatggaccgg attcgggaga
     1621 ggacaaaatc gcgaatctcg ttctttaaag cgcaataggc gatgatatgc catgtaccca
     1681 tgtattgcag caagtggagg ggcagaatat cccggcttgt aacgtttccc gtatggggcg
     1741 accggtaatc tatcctgatc gtgtttccgt aaagcagggc ttcaagggtt ttctgaaaaa
     1801 ttttctcgtt tgtccttgaa tattcgatat tcttgaccga tactttttcg cacaggtcgg
     1861 cgatggatac atacccaggt ccagaaaatc ttgagataac cttgttgatg aaagatttca
     1921 gagaattctt gagatggttg tcgggaatcg atgaggcaag tcggtaggag acaagtagcg
     1981 aggaaagttc tgtttcgttc agccaaaaac cgggaagctc gtaagcatct atgtcgtatg
     2041 cgaagcccct ccgctccggt acgtagtaaa gcggcgcccc caacctgtcc cgcatgaaag
     2101 cgatgtcgcg ctgggccgtt tttcgcgata tctcgaattt ttccgcaaga ctccgggtgt
     2161 tcgggtattt cctttccctg accttggtgt ggaaccagag gaagcgctcg tagatgacct
     2221 tggatgacat aaggcctcca ggatgaatgc tacaacatga acggcgttac gaaaatcaag
     2281 aagaacggcc ggaagggata tttgtgaaat gagttaacgc tgttacgata tgttttgtca
     2341 agaaaattgt tggagaattg atggaagttg gcgcgcaaga gacgaagtca tgcaatctct
     2401 atcggcaggc cgaaaatgtc cttcgcgttc tgtgaagtga cctttccgac ttcctccggt
     2461 gtgattccct tgacctctgc tatcttgcgg gcggtgaaga ctacgtaggc gggttcgttc
     2521 cgttttcccc ggtaggggtg gggtgtgagg tagggggcgt cagtttcgat caggagccgg
     2581 ttcaggggga cgcctttgac gacatcacgc attttctcgg acttggggaa cgtgacgggg
     2641 ccggggatag agatgtagaa cccgaggtcg atacactcca tagccatcgt cctgtcgccg
     2701 gagaagcagt gaacgacgcc cctttttttg cccttccagc ctttcagcat ctcgagcgtt
     2761 tcgcgatggg cttcgcggtc gtggatcacg acggggaggt ccagttcttc cgccagttcg
     2821 agctgttcgc cgaatctctt tatctgaacg tctttagggg agaggtttcg gaagaagtcg
     2881 aggccgattt ccccgtaggc cacgacttta tccgccgatg cgaggcttcg gatcgtgtca
     2941 taggtctcag gatcaatgct tttcacttcg tgggggtgga tgccgacgac ggcgaagacg
     3001 ttttcatgct gcttcgcgat cgcgagagcc ttccggcagt cctccagcgt cgttccgacg
     3061 gttacgatgt attctacccc agcttccagc gcccgtttca ccacttttgc gcggtcccgg
     3121 tcgaactcct tcatttccag gtgcgcgtga gaatcgatgt acatgatgat tcaatccttt
     3181 cttcagaaat cgccttatcc tctcagcctt gggtcgaggg aatcacgtat cccttctccc
     3241 aggaggttat agccgagtac ggtcaatagg atggccaatc ccgggaaaaa ggagagccac
     3301 caggcgatgt cgatgttgtc cttccccgcg gtcaggatgt tcccccagct cggtgtcggc
     3361 ggttgcaccc cgatgccgag aaagctcaga gcggattcgg tcagtatcgc gccagcgacc
     3421 ccgaaggtgg cggcgacgag gatggaggcc atggcgttcg ggaggatgtg gatgaagatg
     3481 atcctcatgt cgcctgcgcc gatggcccgg gcggcctgca cg
//
LOCUS       DAOTMX010000175         3419 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_21838_length_3419_cov_3.341855, whole genome shotgun sequence.
ACCESSION   DAOTMX010000175 DAOTMX010000000
VERSION     DAOTMX010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3419
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21838_length_3419_cov_3.341855"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="P5573_05795"
     CDS             complement(<1..518)
                     /locus_tag="P5573_05795"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRT27608.1"
                     /translation="MKSYRGTPSIFEKASGEKKAELEVRNLSLSFGGVTALRGVDLDV
                     KTGELVAVIGPNGAGKTSLLNTITGYYRAGGGTIRFNGRDITRVATHEAVRLGIGRTF
                     QNIELFPGMTVLSNLMLARHLHCRYRFHSACCFSRAARDEEVRHRRILEEIIDFLEIQ
                     PIRKTLVGSLPYG"
     gene            complement(525..1724)
                     /locus_tag="P5573_05800"
     CDS             complement(525..1724)
                     /locus_tag="P5573_05800"
                     /inference="COORDINATES: protein motif:HMM:NF024825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRT27609.1"
                     /translation="MKGAKFISVWVAVALLGFCCVADTQAASIKVGAAINLTGPASTW
                     GQYHAKGQQDYFRYVNEVKGGVAGRYIEMVLVDTAYKVPEAVSAVRRFAVQDNVDMIA
                     TWGTGEGLAAKPIVQKYMIPTLNYSTGWEILEPPVSYMYLPFGSYRMDCFAILDYIRS
                     VHRGKGPPKVGLLTYNNAYGRSIHKPSQEYAAKAGVNIVAIEEFPPKTVDLTTELLRL
                     QRKGAEYVFMQMLPAAIISALKGADRINYNPLFLGTWTSTDPDFFKMGKGLIRDRLVI
                     QFPGGLPTDESPGIKIMQELWKRYGTVSQFDASYWEGVVIGMIMERAFIRAQQKYGRI
                     DRATINKAMESFRNEDFGGLVPAVTYTPTNHEASFKARIVQVRDDGTYRPLTNFFTPG
                     REKPKLR"
     gene            2288..>3419
                     /locus_tag="P5573_05805"
     CDS             2288..>3419
                     /locus_tag="P5573_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HRT27610.1"
                     /translation="MSERFKRVLGAVTMALLLLFLATPLFASEAELNIPVLSSAQNNL
                     LMVGFLICFAGIVFGYLEYVKVKKLPAHKCMLDVAEVIFQTCKTYLVQQGKFLLILFV
                     FIGCCVLYYFGALQHMGMGDVLLILSWTVIGILGSYGVAWFGIRMNTLANARMAFASL
                     EGKPIKLLNIPLTAGMSIGVLLVCVELIMMLIILVFMPREVAGASFVGFAIGESLGAS
                     ALRICGGIFTKIADIGSDLMKIVFTIKEDDPRNPGVIADCTGDNAGDSVGPTSDGFET
                     YGVTGVALISFIVLGAFPQYQAELLVWIFVMRILMIVTSIASYFINNVIVNGMMAGKE
                     KFNFEAALTSLVWITSIVSIIVTFAVSKWQLGHLPDDLWFKLS"
BASE COUNT          736 a          995 c          858 g          830 t
ORIGIN      
        1 ccgtagggaa gagagccgac gagggtcttc ctgatcggct gaatttcgag aaaatctatt
       61 atttcttcga ggatgcggcg atgtcggact tcctcgtctc tcgcggcccg actaaagcaa
      121 caggcggagt ggaaccggta tcggcagtgg aggtgccgcg caagcatgag attggagagc
      181 acagtcatcc cggggaagag ctcgatattc tggaaagtcc ggcctatacc aaggcgaacg
      241 gcttcgtgcg tcgcgacccg ggtgatgtcc cgtccgttga accggatggt accgcccccc
      301 gcccgataat aacccgttat cgtattgagc aggctggtct taccggcacc gttcgggccg
      361 atgacggcga ccaattcccc cgtcttgacg tcgagatcaa cgcccctgag cgcagtaacc
      421 ccaccgaaac tcagggaaag atttcgaacc tcgagttcgg ccttcttctc cccggatgct
      481 ttctcgaata tcgacggtgt cccgcgatac gatttcatgc cggactacct caacttcggt
      541 ttttctcggc cgggagtgaa aaaattggtc agcggccggt acgtaccatc atcacggacc
      601 tgaacgatcc tcgccttgaa tgaggcctca tggttggtag gagtgtaagt tactgcaggg
      661 accaaaccgc cgaaatcctc gttcctgaaa gactccatcg ccttgttgat ggtggcgcgg
      721 tcgatgcgtc cgtatttttg ctgcgccctt atgaaggccc gctccatgat catgccgatg
      781 accacccctt cccagtacga tgcatcgaac tggctgacag tcccgtaacg tttccacagc
      841 tcctgcatta tcttaatccc aggggactcg tccgtcggca aaccaccggg aaattggatc
      901 acaagccggt ctcggatgag accctttccc atcttgaaaa aatccgggtc tgtagaggtc
      961 caggtgccga gaaacaacgg attataattt atgcggtccg ccccttttaa ggcgctgatg
     1021 atggcggcag gaagcatctg catgaagacg tactccgcgc ccttcctctg caggcgaagg
     1081 agttcggtgg tcaggtcgac cgtcttcggc gggaattcct cgatggctac gatattgacc
     1141 ccggccttcg cggcgtactc ctgactgggt ttgtggatcg accgcccgta agcgttgttg
     1201 tacgttagaa gcccgacttt cggcggcccc ttacctctgt gaaccgaccg aatataatcg
     1261 aggatcgcaa aacagtccat ccgataactc ccaaagggta gatacatgta gctgaccggc
     1321 ggttcgagga tttcccagcc tgtcgaataa ttgagcgtag ggatcatgta tttttggacg
     1381 atcggttttg ccgccaaccc ctcgcccgtt ccccaggtag cgatcatgtc gacattatcc
     1441 tgaacggcaa accttctgac ggccgaaacg gcctccggga ccttataagc cgtgtccaca
     1501 aggaccatct cgatataccg cccggcgaca ccccctttta cttcgtttac atacctaaaa
     1561 taatcctgct gccctttcgc gtggtactgt ccccacgtcg aagccggtcc cgtcagattg
     1621 atcgccgccc ctaccttgat gcttgctgcc tgggtatccg ccacgcagca gaaacccagc
     1681 agggctaccg cgacccaaac cgagatgaac ttggcacctt tcattcctct tccccctctc
     1741 ttttccccgt gttgcttctc cttgcgacca tccatggacc ttttcggttc ctctccagtc
     1801 cagaccggcc gcacataaaa aaggccgcgg ataatccacg gccacgaata aaaaagccgt
     1861 gggcaacctg gcctgcccac ggccctgttc caatctttat ggtcgagagc tcatcaggca
     1921 gaccagcccg ctaaagccga aaaagccggc aacctcacca aatccgcctg taaacttctt
     1981 catcataatg accccactcg acctgagtag gtacttatct aattcggtct cgccgtattg
     2041 tcaagaattt tttgcgggaa caaaaaacct tgacaatcac aggttgctat gctaatcccc
     2101 ccaccggcct taaaacagac tttcttacat aagtaaccgt tgacaacttc gtaaaaaagt
     2161 cggcatcagg cacagtcgga actatgctgg atgtccccct ttcgatgccg gaatgacttt
     2221 ttcggctttt tacggggcgt caccgttttt ttatggtacg aataatacta aagaaggaga
     2281 caagatgatg agcgagcggt ttaaacgtgt tttgggcgcc gtcacaatgg cgcttctatt
     2341 actgttcctc gcaacgccgc tttttgcgag cgaagcggaa cttaacatcc ccgttctgag
     2401 ctcagctcag aacaacctgc tgatggtcgg ttttctcatc tgttttgcgg gcatagtgtt
     2461 cggctatctt gaatacgtaa aagtaaagaa attacctgct cacaaatgca tgctggacgt
     2521 agccgaagtc atcttccaga cttgcaagac ttaccttgtt cagcagggca agtttttgtt
     2581 gatccttttc gttttcatcg gttgttgcgt tctctactat ttcggtgccc tgcagcacat
     2641 gggcatgggc gacgtcctgc tgatcctcag ttggacggtg atcgggatcc tcggttctta
     2701 cggcgtcgcc tggttcggta tccgtatgaa caccctggcc aacgcccgta tggccttcgc
     2761 ttcccttgaa ggcaagccca tcaaactttt gaatattcct ctaacggccg gcatgagtat
     2821 cggcgtcctc ttggtgtgcg tagagctcat catgatgctg atcatcctcg tcttcatgcc
     2881 tcgcgaagtg gcgggggcgt cttttgtcgg ctttgccatc ggtgaatccc tcggcgcgag
     2941 cgccctccgt atctgcggcg gtatcttcac gaagatcgcc gatatcgggt ccgacctcat
     3001 gaagatcgtc ttcacgatca aagaagacga cccgcgaaac ccgggggtca tcgcagactg
     3061 cacgggcgac aacgccggcg acagcgtcgg cccgacgtcg gacggcttcg aaacctacgg
     3121 tgtcacggga gtcgccctga tcagcttcat cgtcctgggt gcatttcccc agtaccaggc
     3181 agagcttctg gtatggattt tcgtgatgcg catcctgatg atcgtgacct cgatcgcctc
     3241 ttatttcatc aacaacgtca tcgtcaacgg catgatggca ggcaaggaaa agttcaattt
     3301 cgaggcggcc ttgacctcac tcgtctggat cacttcgatc gtctcgatca tagtgacttt
     3361 cgccgtcagc aaatggcaac tcggtcatct acccgacgat ctttggttca aactctcgg
//
LOCUS       DAOTMX010000176         3415 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_21862_length_3415_cov_2.903869, whole genome shotgun sequence.
ACCESSION   DAOTMX010000176 DAOTMX010000000
VERSION     DAOTMX010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21862_length_3415_cov_2.903869"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="P5573_05810"
     CDS             complement(<1..429)
                     /locus_tag="P5573_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRT27611.1"
                     /translation="MDFWSRFKKNKLALGGSIIVVLFFVVSIFAPLLSPHDPGEINLQ
                     NVLAPPSVDNPLGTDELGRDVLSRMVWGSRISLKVGFVSTGIAILIGIVLGAVAGYYG
                     RWVDSLIMRTVDIMLCFPTFFLILAVIAFLEPSIWNIMAII"
     gene            complement(444..1418)
                     /locus_tag="P5573_05815"
     CDS             complement(444..1418)
                     /locus_tag="P5573_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRT27612.1"
                     /translation="MLNYILRRLIFMVPLLIGITIICFAVIHIAPGSPTDLQTQMNPR
                     ASAEVKERLRALYELDKPLHEQYLSWLKKLVVLDLGNSFSPDGRPVADKILERIPITI
                     IINALSLGLIIVVALPVGVASAVRRNSLFDKITTVLVFIGFAMPTFWLALLLMILFGI
                     HLGWLPISGIRSLNYEYLPPFSAFADLVKHLILPVFLSAFGGIAGFSRYIRANMLEVI
                     RQDYITTARAKGLSERTVIYKHALRNALLPVITILGLSVPGLIGGSVIFETIFAIPGM
                     GQLFYMSVMSRDYPVIMGILVIGAVLTLVANLLADVCYALADPRIRVQ"
     gene            complement(1422..2759)
                     /locus_tag="P5573_05820"
     CDS             complement(1422..2759)
                     /locus_tag="P5573_05820"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HRT27613.1"
                     /translation="MKIGFVGTGYVGLVAATCFAHMGNDVTCVDVDEKKIASLNKGIV
                     PIYEPGLELLVKKNLEGGRLRFTTDLAEAVANSLFLFIAVGTPPDANGAADLKNVLSV
                     AEGIGRIMNEYKIIINKSTVPVGTADKVRETIDRELKKRNVSVEFDVVSNPEFMKEGD
                     AVNDFLKPDRVVIGVDNVRTAELMKEIYAPFAMDREKVIVMDIKSAELTKYAANAMLA
                     TRISFMNEIANICERVGADVAKVRLGIGSDSRIGYSFIYPGLGYGGSCFPKDVKALIS
                     TAEEARYEPKILKSVDEMNMRQRRLFMEKIIRYHSDKEGLKGKTAAVWGLAFKPNTDD
                     VRESPALEIVRMLLAEGAHVQAYDPEAQKEAKKVLGRAPGVSYFKNAYDALSGADFLV
                     LATEWHFFRNPDFDRMKKLMKSPVVFDGRNQYDPAEMKSRGFVYVCVGRCQQV"
     gene            complement(2821..>3415)
                     /locus_tag="P5573_05825"
     CDS             complement(2821..>3415)
                     /locus_tag="P5573_05825"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HRT27614.1"
                     /translation="ATETLALGINMPARTVVLERLVKFNGEQHLPLTPGEYTQLTGRA
                     GRCGIDVEGHAVVIWNPQDSTSEPVEIAGLADTTIALENVYEKTPHQLCRLFRGLDAA
                     NLIFCFDAGHFNAFSNRSFEQWMEELGPFLGQLHLHDNSGVADEHLPVGDGNFPFARL
                     FSTVEEMKVRPIITLEPHTEENFWKTLKNLETIGLPA"
BASE COUNT          713 a          940 c         1025 g          737 t
ORIGIN      
        1 gatgatggcc atgatattcc aaatagaggg ctcgaggaag gcgatgaccg cgaggatcag
       61 gaagaaggtg gggaaacaga gcattatgtc caccgtccgc atgatgaggg aatccaccca
      121 tcttccgtaa tatcccgcaa cagccccgag gactatgccg atcaggatgg cgatgcctgt
      181 cgagacgaag cccaccttga gagaaatcct cgatccccag accatcctgc tcaggacgtc
      241 ccggccaagc tcgtccgtgc caagcggatt atccaccgag ggcggagcga ggacgttctg
      301 gaggttgatc tccccggggt cgtgaggcga aagcaacggg gcgaagatcg acacgacgaa
      361 gaagagcacg acgatgatgc tccctccgag ggccagtttg tttttcttga atcgactcca
      421 gaaatccatg gcgtattttg ctcttattgt actcttatcc tcgggtcggc cagggcgtag
      481 cagacgtcgg cgagcaggtt ggcaacgagc gtgagcacgg cgccgatgac gagaataccc
      541 atgatgaccg ggtaatcccg cgacatgacc gacatgtaaa aaagctgtcc catgccgggg
      601 atcgcgaaga tggtttcgaa gatcacgctc ccgccgatga gaccgggcac cgagaggccg
      661 aggatcgtga tcactggcag cagggcattc ctcaatgcgt gtttgtagat gaccgtcctt
      721 tcgctcaggc cctttgccct cgccgtggtg atgtaatcct gcctgatgac ctcgagcatg
      781 ttggcccgta tgtaacggga gaagcccgcg atgcccccga aggcggaaag gaacacgggg
      841 aggatcaggt gcttgacgag gtccgcgaag gcgctgaacg gcggcaggta ttcgtagttg
      901 agggaacgga tccccgagat ggggagccat cccaggtgga tgccgaagag gatcatcagc
      961 aggagggcga gccagaacgt cggcatggca aagccgatga agacaagcac ggtcgtgatt
     1021 ttgtcgaaga gggaattccg gcggacggcg gaggcaactc cgacgggaag ggcgacaacg
     1081 atgatgaggc cgagggaaag ggcgttgatg atgatggtga tagggattct ttccagtatc
     1141 ttatccgcca cgggtcggcc gtcgggagaa aaggaattcc ccaggtcgag aacgacgagc
     1201 tttttaagcc aggataggta ctgctcatgg aggggcttgt cgagttcgta aagtgccctc
     1261 aacctctctt tcacctccgc cgaagcacgg gggttcatct gggtctgcag gtcggtcggg
     1321 gagccggggg cgatatgtat gacggcaaag cagatgatcg ttattccgat cagcaaaggc
     1381 accatgaaga tgagtcttcg caagatatag ttcagcaacg ctcagacctg ttgacagcgg
     1441 ccgacgcaga cgtacacgaa gcctctgctc ttcatctctg ccgggtcata ctggtttctc
     1501 ccgtcgaaga cgacgggact cttcatgagt tttttcatcc ggtcgaaatc ggggttcctg
     1561 aaaaagtgcc attccgtcgc gaggaccagg aaatcggctc ctgagagcgc gtcgtaagcg
     1621 tttttgaagt atgagacccc gggagcgcgt cccaagacct tcttcgcctc tttttgtgct
     1681 tccgggtcgt aggcctggac gtgcgcgcct tcggcgagca acatgcggac gatctccagt
     1741 gcgggcgatt cccggacgtc gtcggtgttc ggcttgaagg ccagtcccca gaccgcagca
     1801 gtcttacctt tgaggccctc tttgtctgaa tggtagcgga ttatcttttc catgaagagg
     1861 cgtctctgtc tcatgttcat ctcatcgacg gacttcagga tttttggctc gtaccgtgcc
     1921 tcctcggcgg ttgaaatcag cgccttcacg tccttcggaa aacaggagcc tccgtatccg
     1981 agtccggggt agatgaatga ataaccgatc ctactatccg acccgatccc cagccgcacc
     2041 tttgccacgt cggcgcccac cctttcgcag atgttggcta tctcgttcat gaacgatatc
     2101 ctcgtcgcca gcatcgcgtt tgccgcgtac ttggtcaatt cggcgctctt gatgtccatc
     2161 acgatcacct tttcgcggtc catcgcgaag ggggcgtata tttctttcat caactcggcc
     2221 gtccgtacgt tgtcgacgcc gatgacgacg cggtcgggct tcagaaagtc gttgacggcg
     2281 tcgccttctt tcatgaattc ggggttggag acgacgtcga actcgacgga gacattgcgc
     2341 ttcttcagtt ctctgtcgat cgtctctctg accttgtccg cggtgccgac ggggacggtg
     2401 gatttgttga tgatgatctt gtattcgttc atgatacgcc ctatgccctc ggcaacggag
     2461 aggacattct tgaggtcggc ggccccgttg gcatcaggag gggtcccgac ggcgatgaac
     2521 aggaagaggg aattcgccac cgcctctgcg aggtcagtcg tgaatctcag tcggccgccc
     2581 tccaagttct tctttaccag aagctcgagg ccgggctcgt atatcgggac gatgccctta
     2641 ttcagggaag cgatcttctt ctcgtctaca tcgacacagg tcacgtcgtt acccatgtgg
     2701 gcgaaacagg tcgcagcgac aagccctaca taacccgtac cgacaaaacc gatcttcatg
     2761 tctcctccgc agaattactt tgggctaaag gcaaaaggct gtactccggc gagagcgccc
     2821 ttatgctgga agccctattg tttcaaggtt cttgagtgtc ttccagaaat tctcttccgt
     2881 gtggggctcg agggtgatga tcggacggac cttcatctct tcgaccgtcg agaaaagcct
     2941 ggcgaagggg aagttcccat cgccgacggg cagatgctca tcggcgacgc cgctgttgtc
     3001 gtgcaggtga agttgcccca ggaagggacc gagttcttcc atccactgct cgaagctgcg
     3061 gtttgaaaag gcgttaaaat gtccggcatc gaaacagaag atcagattcg ccgcgtcgag
     3121 gccgcgaaaa agacggcaga gttgatgagg cgttttttcg tacacgtttt cgagggcgat
     3181 cgtcgtgtcg gccagcccgg cgatctccac cggctccgac gtggagtcct gcgggttcca
     3241 gatcaccacc gcatggcctt cgacgtcgat gccgcagcgg ccggcccgac cggtcagctg
     3301 ggtgtactcc cccggtgtga gcggcaggtg ctgttcgccg ttgaacttca ccaaccgctc
     3361 cagcacgacg gtgcgagccg gcatgttgat gcccaaagcc agtgtctccg tggcg
//
LOCUS       DAOTMX010000177         3410 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_21900_length_3410_cov_3.182712, whole genome shotgun sequence.
ACCESSION   DAOTMX010000177 DAOTMX010000000
VERSION     DAOTMX010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21900_length_3410_cov_3.182712"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="P5573_05830"
     CDS             complement(<1..346)
                     /locus_tag="P5573_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="With MotB forms the ion channels that couple
                     flagellar rotation to proton/sodium motive force across
                     the membrane and forms the stator elements of the rotary
                     flagellar machine; Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HRT27615.1"
                     /translation="MLVIIGCAVVILCVAGGFLIEGGNLNILFQPAELLIIGGAAFGG
                     FLVATPAAAVKQVIAGVMETLKGDPYTKKDYLDVLQLLGEIFYKIRKQGLVAVEADVD
                     NPGESAIFSKYPG"
     gene            complement(337..897)
                     /locus_tag="P5573_05835"
     CDS             complement(337..897)
                     /locus_tag="P5573_05835"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRT27616.1"
                     /translation="MEENNAKDVKSREYSRVDACIPMDFALLTEEELPEVRSTISRQP
                     YLLGVTIPDDVEDKALSDWLHLFNSKLDFIIHSLSVDREELPALPFRHVNISASGMRF
                     TAKEGYEKGQVLSIRMVLQKQRPLSLHLCGQVVESLEISDSEFEVAVKFIFIDDEIRD
                     ELVRFVFEKQREILRGKRDSEFRRCS"
     gene            complement(929..1573)
                     /locus_tag="P5573_05840"
     CDS             complement(929..1573)
                     /locus_tag="P5573_05840"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRT27617.1"
                     /translation="MIVRCWMTSHPLVVADEEMPIEEARLLMRRHGVRRLPVMSGSEL
                     KGIVTLSDISAARPEGAAADDKPPQESKRLRVCDVMSKKVLTCDANCPLEEVGALMRR
                     EKVGALPVMNGNRLVGIITESDVLDALVSITRVGKDTRRLYLRVPLARKCDIFREVID
                     IGIRYGIELYTLLTHPIRDSDSHLVMLKFSGDRAEGFVQALWESGYQMLITDNP"
     gene            1755..2507
                     /locus_tag="P5573_05845"
     CDS             1755..2507
                     /locus_tag="P5573_05845"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRT27618.1"
                     /translation="MNRDMLWHDRFPREDERPATVTKKWQEEIKLAYRFDLERELDSL
                     GILDKAREIHTELGDNAAISYIRSSFRLLSKVYHPDLNPNKSERAKAIQQRLNEVNQI
                     IGRMNDDELLALLGKNPKITAADKKKILVVEDEFGLQETLRNIFLMEGYETRIAVDGQ
                     NGYAVYRQFKPHLILTDVVMPEMNGIDLVRRVRKEDRQIKVIFMSGFFGIKKLKQDLD
                     DEILKHGYRTLSKPFKISDLLDLVQQYLDEPV"
     gene            complement(2614..>3410)
                     /locus_tag="P5573_05850"
     CDS             complement(2614..>3410)
                     /locus_tag="P5573_05850"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HRT27619.1"
                     /translation="PLGAQRRVREKEHLIIIGFGVNGRNLARAARAAGIPYAIVEMNP
                     AAVKEERKKGERIYFGDATQEAILQHVNIKEAKAVAVVINDASATRRIVEVVRRLNPK
                     VYLIVRTRFVQETGPLLELGADEVIPEEFETSVEIFSRVLAKYLLPKEEIERFVSEIR
                     AEGYGMLRSLSREATSCLDLELCLPNVEIKSFRIMGDSPLVGKTLAETQMRKIYGVTL
                     LAIRRKDRILSNPDPETVFRPNDILFVVGKPDRIAEVITLFSQGPS"
BASE COUNT          771 a          985 c          924 g          730 t
ORIGIN      
        1 cgccggggta tttgctgaaa atggcgcttt ctccgggatt gtctacatcc gcttccacgg
       61 cgacgagccc ctgtttacgg attttataga atatctcacc caggagctgg aggacgtcaa
      121 ggtagtcttt ctttgtgtag gggtcgccct tcagggtctc catgacgccc gcaatcactt
      181 gtttaaccgc cgcagcagga gtggcaacga gaaaaccccc gaacgccgcc ccgccgatga
      241 tcaggagttc ggcgggctgg aagaggatgt tgaggttgcc gccctcgata agaaaaccgc
      301 cggcgacgca caggatgaca accgcgcaac cgattattac gagcatcttc tgaactcgct
      361 gtctcttttt cccctgagga tctccctctg cttctcaaag acaaaccgga cgagttcgtc
      421 cctgatttcg tcgtcgatga aaatgaattt gacggccacc tcgaattcac tgtctgatat
      481 ttccagggat tctacgactt gtccgcacag atgcaaggac agagggcgtt gtttttgcaa
      541 aaccatcctg atggacagta cctgaccttt ctcgtaaccc tctttggctg tgaacctcat
      601 gccgctcgca ctgatgttca catggcggaa aggaagggca ggcagttcct ccctatcgac
      661 ggacagtgaa tggatgataa aatccagctt agaattaaaa agatggagcc agtctgaaag
      721 ggccttgtcc tccacgtcgt cggggatagt caccccgagc agataaggct gacgagaaat
      781 cgtcgacctg acctccggca gctcctcctc cgtcagtaga gcgaaatcca tcgggataca
      841 ggcatcaacc cgtgaatact cgcgggactt cacatcttta gcattgtttt cctccatctc
      901 ctatccttcc tctcttttta ggcggcattc agggattgtc ggtgatcagc atctgataac
      961 ccgattccca caaggcctgg acaaatccct ccgctcggtc gccgctgaac ttcagcatga
     1021 cgagatgact gtcggaatcg cgtatcggat gagtcagcaa ggtataaagt tcgatgccgt
     1081 atcggatgcc gatatcgatg acctcacgga aaatgtcaca tttccgggcg agagggaccc
     1141 tgaggtacag cctcctcgta tcctttccga cccgggtgat actgacgagc gcatccagga
     1201 cgtcggactc cgtgattatg cccacgagcc tgtttccgtt catcacgggc agtgctccga
     1261 ccttctcgcg gcgcatcaag gcgccgacct cttcaagggg acagttcgcg tcacaggtca
     1321 acaccttctt cgacatgacg tcgcaaaccc ggaggcgttt gctttcctgg ggtggcttgt
     1381 cgtcggcagc ggcaccttcg ggacgggcgg cgctgatgtc ggacagcgtg acgatgcctt
     1441 tcaattcgga gccgctcatg accggcagcc gacggacccc atggcgtctc atcaagagtc
     1501 tggcctcctc gatcggcatc tcctcatcgg caacgacgag gggatgactg gtcatccaac
     1561 agcgcacgat cattcgtcaa cctcaccttc cggacgattt agtattttcc cgtcgtggcg
     1621 cagacaacgc cgccttgaaa tcatgcaaaa tcccagccat gcgacgggga gggcggaagg
     1681 ggtgacaacc ggcggaggta tagtctccgg cctctcgaat cccagacgga gaaggccttg
     1741 aaaaggcttg attgatgaac cgtgacatgc tatggcatga ccgtttcccc cgggaagacg
     1801 agagacctgc gactgtaaca aaaaaatggc aggaggaaat aaaattggca taccggttcg
     1861 acctggagag agaactcgat tccctcggaa tcctcgataa ggccagggaa atacatacag
     1921 agctggggga caacgccgct atttcctaca taaggtcgag ctttcgcctg ctttcgaagg
     1981 tctaccaccc cgacctgaac ccgaacaagt cggaacgggc caaggcgatc cagcagcggc
     2041 tcaacgaggt aaaccagata ataggccgga tgaatgacga tgaactcctc gcgttgctcg
     2101 gaaagaatcc caaaataacc gcggcggaca agaaaaaaat cctggtggta gaggacgagt
     2161 tcgggcttca agaaaccctc aggaacatct ttctcatgga aggctatgaa acgaggatag
     2221 ccgtcgacgg gcagaacggt tacgcggtat accgccagtt caagccccat ctcatcctga
     2281 ccgatgtcgt catgccggag atgaacggca tcgacctcgt gcgcagggtc aggaaggaag
     2341 accgacagat aaaagtgatc ttcatgagcg gttttttcgg gataaagaag ctaaagcaag
     2401 acctggacga cgagatcctg aaacacggct accgaacgct ttcgaaacct ttcaagatca
     2461 gcgacctgct cgatttggtg cagcagtacc tggacgaacc ggtctgaacc gtccaccccc
     2521 cacggatacc cgtcaaaagt ctgccatagg tgtcgggatt cctacctccg ccgggaacgg
     2581 caaaggccgt atgacatcgg tgcactctgg cgttcacgac ggtccctgcg aaaagagggt
     2641 gatgacctcg gcgatccggt ccggtttccc gacgacgaag aggatgtcgt tcggtcggaa
     2701 aacggtctcc gggtcggggt tcgacaggat gcggtccttt ctccggatgg caaggagggt
     2761 cacgccgtag atttttctca tctgggtctc cgccaaggtc ttcccgacga gcggcgaatc
     2821 gcccatgatg cggaaggact tgatttccac gtttgggagg cacagttcca ggtcgaggca
     2881 tgaggtcgcc tcccgagaaa gactccgcag catgccgtag ccttcggccc tgatctccga
     2941 gacgaatctc tcgatctcct ctttcggcag gaggtacttg gccaggactc tgctgaatat
     3001 ctcgacggac gtctcgaatt cctccgggat gacttcgtcg gcgcccagtt ccagcagagg
     3061 gcccgtttcc tgcacgaagc gcgtccgcac gatcaagtag accttcgggt tcaacctccg
     3121 gacaacctcc acgatcctgc gggtggccga agcatcgttg atcacgaccg cgaccgcctt
     3181 tgcctccttt atgttgacat gctggaggat cgcctcctgg gttgcgtcgc cgaagtagat
     3241 gcgctcacct ttcttcctct cttcttttac tgccgcgggg ttcatttcca cgatcgcata
     3301 cggaataccc gccgcccggg ccgcccgggc cagattccgc ccgttcacac cgaaaccgat
     3361 tatgatgagg tgttcttttt cacggacccg tctctgcgct cccaacggat
//
LOCUS       DAOTMX010000178         3396 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_21998_length_3396_cov_2.876384, whole genome shotgun sequence.
ACCESSION   DAOTMX010000178 DAOTMX010000000
VERSION     DAOTMX010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21998_length_3396_cov_2.876384"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="P5573_05855"
     CDS             complement(<1..640)
                     /locus_tag="P5573_05855"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRT27620.1"
                     /translation="MAVVLIVDDEPKQREILKIILADEGYETFTASSGEEAARIARSY
                     NPDVVLTDLKMKGMDGISLMSRVKTIEPEPAVVLMTAFGDVPTAVEAMKKGAFDFLTK
                     PLDKDVVLLTIKRAVERTELLKRSAMLQKALYDKFSLEGIVGRSRAISEVITVVKKVA
                     ASPVTVLILGESGTGKELVARAIHYNSPRSGKPFTAINCAAIPETLLESELFG"
     gene            complement(666..2120)
                     /locus_tag="P5573_05860"
     CDS             complement(666..2120)
                     /locus_tag="P5573_05860"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRT27621.1"
                     /translation="MDNHLKIDILKNFFLGLSLNKKLILMMLCLSALLLSILLILHWQ
                     TERALVREFENQTAELTKAIQVGVEKVTGNYSTKESGLESYLKTLKSKGIEEISVISA
                     TEHIIASSNPTKVGLPVGPKRKELIIKAALGTQISEEGKTYNVMIPVIAGRTHYGYIH
                     LQINAEDISGMQKRNTIKMIVATLLVIAFGIFISIILALWYTEPIRQVVEASKRVAAG
                     DLDVKLPVDRKDELGDLTENFNNMVQRLREKKQLEERLREAEHLSALGQLARTIAHEI
                     RNPLNYISLCIDHIKAKYSPEKGAQAASFETLVSGIKQEIHRLDKLVTEFLDYGKPLK
                     MNIRCAGIEQVLKDVLDIVKTKADSEKVAIVEEYEALPEVMLDIELMKTCIFNIVTNA
                     FHAMPEGGTLTVRTTTENGNLRLDISDTGAGIPRENIGKVFEPFFTTKANGLGLGLAI
                     TKRIIEEHGGSVEFFSVENKGSDFVIHLPLTNPQ"
     gene            2306..>2894
                     /locus_tag="P5573_05865"
     CDS             2306..>2894
                     /locus_tag="P5573_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HRT27622.1"
                     /translation="MDCLFDFVDIVLHLDRYLDLIIRDYGTWTYGVLILVVFCETGLV
                     VTPFLPGDSLLFAAGTFAALGSLEVEWIALSLGLAAVMGDSSNYWVGRYVGPRVFTRE
                     RSRLFNKDHLMRTQRFYERYGRKTIILCRFIPIIRTFAPFVAGIGRMPYGVFLSFSII
                     GTTDTDYKGDLDAVYADRDDVDYILDAINVQFPGVN"
     assembly_gap    2895..2904
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2905..3106
                     /locus_tag="P5573_05870"
     CDS             <2905..3106
                     /locus_tag="P5573_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27623.1"
                     /translation="IGTTCWISVFVFGGYFFGNIPIVKGNFTAVILGIIFVSIMPGVL
                     EFIRHRYGNKVKSGNRTLNDEL"
     gene            3126..>3396
                     /locus_tag="P5573_05875"
     CDS             3126..>3396
                     /locus_tag="P5573_05875"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRT27624.1"
                     /translation="MRITFLGATREVTGSCCHISVDSMDILVDCGMHQGKDGDYKNRR
                     AFPFEPRNIGCVFLTHAHIDHSGLLPRLVKQGFEGRIIATPATADL"
BASE COUNT          661 a          936 c          934 g          855 t
ORIGIN      
        1 agccgaagag ttcgctttcg aggagggtct ccggtatggc ggcacagttg atggccgtga
       61 atggtttccc gctccggggg ctgttgtaat gaatggccct ggcgacgagc tccttgccgg
      121 taccgctctc ccccaagatc aggacggtga ccgggctggc ggcgactttt ttgaccaccg
      181 tgatcacttc gcttatagcc cgggaacggc cgactatccc ttcgaggctg aacttgtcgt
      241 acagcgcctt ctggagcata gccgacctct tcaggagctc cgtcctctcg accgccctct
      301 ttatcgtgag caggactacg tccttgtcta gcggttttgt taggaagtcg aaggccccct
      361 tcttcatcgc ctccaccgcc gtcggtacgt ccccgaaggc ggtcatgagg acgaccgcgg
      421 gttcgggttc gatagtcttc acccgactca tcaggctgat gccgtccatc cctttcatct
      481 tgaggtcggt gagcaccacg tcggggttgt aagaacgggc gattctcgcc gcctcctcgc
      541 cggacgaggc ggtgaaggtc tcgtatccct cgtcggcgag gataatcttg agtatctcgc
      601 gctgcttggg ttcgtcatct acgatgagta cgacggccat gatcagtctg ctgttctcct
      661 ggtttctact gaggattggt caacggcagg tgaatgacga aatcgcttcc cttattttcg
      721 acgctgaaaa attcgaccga cccgccatgt tcctcgatta tccgtttggt tatagcaagg
      781 ccgaggccga ggccgtttgc cttcgtggtg aagaagggct cgaatacctt gccgatattt
      841 tccctcggta tgcccgcgcc ggtgtcgctg atgtccaggc gaaggttgcc gttttccgtt
      901 gtcgtgcgga cagtgagagt cccaccctcg ggcatggcat ggaaggcgtt tgtcacgatg
      961 ttgaagatgc aggtcttcat cagttcgatg tcgagcataa cttccggcag ggcctcgtat
     1021 tcctcgacga tggcgacctt ttccgaatcg gccttcgtct tcacgatgtc cagtacgtcc
     1081 ttcaagacct gctcgatgcc ggcgcagcgg atgttcatct tcagcggctt tccgtaatcg
     1141 agaaattccg taacgagttt gtcgagcctg tgaatctcct gcttgatgcc ggataccagg
     1201 gtttcgaagc tcgccgcctg agcgcccttt tcaggggagt atttggcctt gatgtggtcg
     1261 atgcaaaggc tgatgtaatt caaggggttc cttatctcgt gagcgatcgt ccttgccagt
     1321 tgccccagtg cggaaagatg ctccgcttcc cggagtcgct cctcaagctg tttcttctcc
     1381 ctcaacctct ggaccatgtt gttaaaattc tctgtgaggt caccgagttc gtctttgcgg
     1441 tcgacgggaa gcttcacgtc caggtcccct gccgcgaccc tcttcgaggc ctcgaccacc
     1501 tggcgaatcg gctccgtgta ccagagggca aggataatgg atatgaagat accgaaagca
     1561 atgacaagca gtgttgcgac gatcatcttg atggtgttgc gtttctgcat accggagatg
     1621 tcttcggcgt tgatctggag gtggatgtag ccgtaatggg tccggccggc gatgacgggg
     1681 atcatgacgt tgtaagtctt gccctcctcg gatatctgcg tcccgagcgc cgccttgatg
     1741 atcaactcct ttctctttgg tcccaccgga agtccgactt tcgtggggtt ggagctggcg
     1801 attatgtgtt cggtagcgct gatgactgaa atctcttcga ttccttttga cttcaaggtc
     1861 ttcaggtagg attccagccc tgactccttg gtagaataat tgcccgtaac cttctcgaca
     1921 ccgacctgga tggcctttgt caattccgcc gtctgatttt cgaactcacg cacgagcgcc
     1981 cgctccgtct gccagtggag aatcaaaagg atcgagagaa ggagggcact gagacagagc
     2041 atcatgagga tgagtttttt gttcagggaa aggccgagga agaaattttt caagatgtct
     2101 attttcaagt gattgtccat aaacactcac cacgcttcac ccggcacgga acggcaagaa
     2161 tgcgaaaaca acaacgtctc gtagctgcgt tattttaaga aaatctacca ccggccggaa
     2221 tgacttgtca accgttcacg ggcgcctttt atccttgact ttttcccctc catgactcaa
     2281 aaagcagggg cgaccggagg agaggatgga ctgtcttttt gattttgtcg atatcgtact
     2341 gcacctcgac cgctatctgg acctcatcat cagggattac ggaacctgga cgtacggcgt
     2401 cttgatccta gtggtctttt gtgagacggg tcttgtggtc acaccttttt tgcccgggga
     2461 ctcgctgctc ttcgcggcgg gcacctttgc ggccctcggt tcgctcgagg tagaatggat
     2521 tgccctgtcc ctcggcctcg ccgccgtgat gggggattcg tccaattact gggtcggtcg
     2581 ttacgtaggc ccgagggttt ttacccggga aaggtctcgc ctcttcaaca aggatcacct
     2641 catgaggacg cagcggttct acgaaaggta cggccgcaag actataatcc tctgccgctt
     2701 catccccatc atccgcactt tcgccccctt cgtggccggg atcgggcgca tgccctacgg
     2761 ggtctttctc tccttcagca tcatcggcac gaccgatacg gactacaagg gcgatctcga
     2821 tgccgtctat gccgatcggg acgatgtcga ctacattctg gatgccatca atgtgcagtt
     2881 ccccggcgtc aaccnnnnnn nnnncatcgg cacgacctgc tggatatcgg tgtttgtgtt
     2941 cggcggttat ttcttcggta acatcccgat agtgaaagga aactttacgg ctgtcatcct
     3001 ggggataatt ttcgtctcca tcatgcccgg cgtattggaa ttcatccgcc acagatacgg
     3061 caataaggtg aagagtggga atcggacact caacgacgaa ctttaaaaca gggagagcaa
     3121 tggacatgcg aataacattc cttggagcga caagagaagt gaccggctcc tgttgtcata
     3181 tctcggtaga cagcatggat atcctggtcg actgcgggat gcatcaggga aaggatggcg
     3241 actacaagaa tcggagggct ttccccttcg aaccgcgaaa cataggttgc gtgttcctca
     3301 cccatgccca catcgaccat tcgggcctcc tgccccgcct cgtgaagcag gggtttgagg
     3361 gtcgtataat cgccaccccg gcaacggccg accttg
//
LOCUS       DAOTMX010000179         3382 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_22113_length_3382_cov_4.168320, whole genome shotgun sequence.
ACCESSION   DAOTMX010000179 DAOTMX010000000
VERSION     DAOTMX010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22113_length_3382_cov_4.168320"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="P5573_05880"
     CDS             <1..240
                     /locus_tag="P5573_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27625.1"
                     /translation="MYVIETEYKLRDNMSGIRYFPCFIAGDNKGNEDILWGATFSIVL
                     SFLKTVFDFELPNLKGKRIIKKVLEPEYLTGNQKR"
     gene            260..925
                     /locus_tag="P5573_05885"
     CDS             260..925
                     /locus_tag="P5573_05885"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRT27626.1"
                     /translation="MNFKAIIFDFDGTLVKLGLDFPLMRASVLDLLLSYGVAPDGLDG
                     LYVLEMIEAGRKMIEEASPGEGDRFFQKAYEAIRQIEVAGARRGELVTGVRDMLSSLK
                     ERGIAVGIATRNCRDAVLESFPDIQSFCDTLVTRESTRRVKPDPEQILIALRALHVDA
                     SLSAMVGDHPMDIEAGRRAGSFTVGVLTGYSSADALTAAGANLIIERATHITRYLRTK
                     KKG"
     gene            1014..1087
                     /locus_tag="P5573_05890"
     tRNA            1014..1087
                     /locus_tag="P5573_05890"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1046..1048,aa:Gln,seq:ctg)
     gene            1389..2399
                     /locus_tag="P5573_05895"
     CDS             1389..2399
                     /locus_tag="P5573_05895"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRT27627.1"
                     /translation="MKIDCYTHFSFERYLAFLEKNSKNSFPYATLFRYLKTMLDVGSR
                     LALMEKYGVDKHVIVPFPWIESVKEVYDDPIKSKEAACILNDELAGLVGKYPDRFIPV
                     AAIPTTNRDTMLSEYERAIHKLKFKGIYLVVGPTVRPVDDPMYGDLFQQAVRDQVPVW
                     LHPSRPVTYSDYVTESTSKYGIWQSIGWLMDSSTAMIRLTLSGTFEKYKGIKFIIHHH
                     GALIGLFAKRLNAGFRLFKNIEAFRSETNLTEPYIDHFKNFYVDTATQGFEPLLIQNA
                     CMFFGVGHVLFGTDVPFDEKGGYDFTEQTISSIDAALISDADKERIFYKNIAQILNLG
                     VD"
     gene            2839..3114
                     /locus_tag="P5573_05900"
     CDS             2839..3114
                     /locus_tag="P5573_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27628.1"
                     /translation="MKKTLTTIILAGFMTLLTATFCLAEYSATGTENFPYFHLGALII
                     GGLSIISLRTKYRKIYMTEAMGALALYTVLIALFTAPVVEAIKNIVG"
BASE COUNT          852 a          925 c          838 g          767 t
ORIGIN      
        1 atgtatgtga tcgaaacaga gtataaactg cgggacaata tgagcggcat acgctacttc
       61 ccctgtttca tagcagggga caacaaggga aacgaggaca tcctctgggg agcgacattc
      121 agtatcgtcc ttagtttcct caagaccgtc ttcgatttcg agcttcccaa cctgaagggc
      181 aaacgaatca tcaagaaggt tctggaaccg gaatacctga cgggaaacca gaaaagatag
      241 tgcagggtta cgtgatacat tgaactttaa ggcgatcatc ttcgatttcg acggaacact
      301 ggtgaagctt gggctcgact tccctctgat gagggcatcc gtccttgacc tcctcctttc
      361 ctacggagtt gccccggacg gcctggacgg tctctacgtc ctcgagatga tcgaggcggg
      421 acgaaagatg atcgaggagg cctccccagg agagggggac cggtttttcc aaaaggccta
      481 cgaggcaatc cggcagatcg aggtcgccgg cgcccggcga ggcgaactcg ttaccggcgt
      541 gagagatatg ctttcctcgt tgaaggagcg cggcattgcg gtcggaatcg cgacgagaaa
      601 ctgccgagac gccgttctgg aatccttccc cgacatacaa tccttttgcg acacgctggt
      661 cacccgcgag tcgacgcgaa gggtgaaacc cgacccggaa cagatactca tagcgctgag
      721 ggcccttcac gtcgatgcca gcctctccgc catggtgggc gaccatccca tggacatcga
      781 ggccgggagg cgcgccggtt ctttcaccgt cggcgttttg accggctatt cgagcgccga
      841 cgcccttacc gcagccgggg caaacctcat aatagagcgc gccacccaca tcactcgcta
      901 tcttcggaca aagaaaaaag gttgacagca aaaaaaggcc gcactatagt ctcgccacta
      961 tccggtgacc cgccaccttg aaaaggcggc tgtgcggggc cgttctcgtt cattggggga
     1021 tcgttcaacg gcaggacaac ggactctgac tccgtgaatc gtggttcgaa tccacgtccc
     1081 ccagccattg gataatcaag aggttgtgag tctcaggagt cggtttccag gccctgatct
     1141 tctgtggttg cgctaaacct atcccggtca ccgtccatca actcgaaccc cgtactcagg
     1201 tgcactctga ggaatgctac gcatggctcc acaagaagtc taccgtcaac atcctcttgc
     1261 cgcaggcatt gaacaatgca agaaactccc ttgccggacc ttccgagtcc aaaacaactg
     1321 cagtacatta taggcggcta cgcctgacac ggatgaaccg ataggagaac taaagcacgg
     1381 agggaaagat gaaaatcgat tgttacaccc atttttcatt cgaaaggtat ttggcgttct
     1441 tagagaaaaa ttctaaaaac tcgtttcctt acgcaactct tttccggtac ctaaaaacta
     1501 tgctcgacgt cggtagccgc ttagccttga tggagaaata cggtgtcgat aaacacgtta
     1561 tcgttccttt cccgtggatc gaatccgtta aagaagtcta tgatgacccc atcaagtcga
     1621 aggaggccgc ttgtatattg aacgatgaat tggccggtct ggtcggtaag tacccggaca
     1681 gattcattcc cgttgccgcc atcccgacga ctaatcgaga tacgatgctg tcagaatatg
     1741 aacgcgccat ccacaaacta aaattcaagg gtatctatct cgtggtaggg ccgacggtga
     1801 ggcccgtcga tgaccccatg tacggcgacc tgtttcagca ggccgtgcgc gatcaggtcc
     1861 ctgtatggct tcacccgtct cgaccggtaa cgtacagtga ttacgtaacc gaaagtacgt
     1921 caaagtacgg catttggcag tcgatagggt ggctcatgga ttcgagtacc gccatgataa
     1981 gattaaccct ttcaggcaca ttcgaaaaat acaagggcat aaagttcatc atccaccatc
     2041 acggcgccct catcggacta tttgcgaaac gcctaaatgc aggtttcaga ctcttcaaaa
     2101 atatcgaggc cttcagaagt gaaacaaacc tgaccgaacc ctacatcgat catttcaaaa
     2161 acttctatgt cgatacggcg acacaggggt tcgaaccgct gctcatccag aatgcctgta
     2221 tgtttttcgg tgtcggtcat gtactcttcg ggacggatgt ccccttcgac gaaaagggcg
     2281 gctatgattt caccgagcaa acaataagtt caatcgatgc cgccttgatc tcagacgccg
     2341 acaaagagag aattttttat aagaatatcg cccagatact caatctcgga gtagattgag
     2401 gcgattgatc ccgatttagc ccacaagcaa gtcttttgcg catcccggct atggcaaggc
     2461 tgcttcccgt taatacaata ccgggggcaa ccgtggcgtg tagtgcaaca ctaccggccg
     2521 agggatggcc cgttctcatc gccgcgcccc gacgcatttc ctcgcgttct ccatttttcc
     2581 ccctgagacc tgacctcccc tccagagatt caaggcttta atgtttcatt caacgccttt
     2641 taacctttca tcgaagaaat agcgcgtttt tccgctggga tcgactgtgt ggtttcggcg
     2701 cggttcatag aatacgctgt attttcaaca tgatgtatca tgccacatcc ttctgtgcct
     2761 gcctggcatg attatttcaa tagataaagt cgaccggaat aaaaaaagaa ccggcaccag
     2821 aaaggaggaa caaagaccat gaaaaagaca ctaaccacga tcatcctggc gggcttcatg
     2881 accctgttga cagcaacttt ttgccttgcc gaatactcag caacgggcac ggagaacttc
     2941 ccttacttcc acctcggcgc actgatcatc ggcggactca gcatcatctc cctcaggacg
     3001 aagtatagga agatctacat gaccgaagcc atgggtgcgt tggcacttta cactgtcctg
     3061 attgcgcttt tcacggcacc cgttgtggaa gctataaaga acatcgtcgg ctgaaaaaca
     3121 agaaacataa gctgggcttc ggtttctggg gcgcccggac gtcaaaaatg aaagggtgcc
     3181 ccggccgagg aaagtaggca gcaaaggagg taacaagcca tgatcaagac aaccgaaaaa
     3241 ttccgaggtt attcgcagat ccttctcctg agcgcctgga gcttcgtcat agtgctttcg
     3301 tccttcctct tcctctacat cggttactgg gtcgatagga tgctcaacac cgccccgtcc
     3361 ttcatgttcg gcctcttcct cc
//
LOCUS       DAOTMX010000180         3344 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_22441_length_3344_cov_3.753116, whole genome shotgun sequence.
ACCESSION   DAOTMX010000180 DAOTMX010000000
VERSION     DAOTMX010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22441_length_3344_cov_3.753116"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1758)
                     /locus_tag="P5573_05905"
     CDS             complement(<1..1758)
                     /locus_tag="P5573_05905"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HRT27629.1"
                     /translation="MGRTLTQKIIEDHLISGRFEPGTEIGIRIDQTLTQDATGTMAYL
                     QFEAMGLERVATELSVSYIDHNTIQVGFENADDHRYLQSVATKYGIYLSRAGNGICHQ
                     VHLERFGKPGKTLLGADSHTPTGGGLGMIAIGAGGLDVALAMGGRPFYLICPRVVQVR
                     LAGKLQPWVSAKDVILKVLEIFTTKGNVNTVFEYTGEGVEGLSVPERATIANMGAECG
                     VTTSIFPSDEVTRVFLRSQGREKDWRGLEPDGDAVYERVVDIDLSRLVPLAALPHSPD
                     NIAAVKDIAGVPVDQVCIGSCTNASYRDLAIVAEMLRGKTVHPDVSFVLSTGSRQVLL
                     NLARQGYLEILAAAGARIMESACGFCIGNSQSPMSGGVSLRTSNRNFMGRSGTKDARV
                     YLVSAETAAAAVITGRITDPRELGIPYPKVEMPETFIVDDRMIIRPEEAGKSRFRDVY
                     RGPNIGTPPVNEPLPDRIDGEVTIVVGDKITTDHIIPAGARMKYRSNIPKYSEFVFES
                     VDPGFAGRAAKIKEEGRHNIIVAGLSYGQGSSREHAALCPMYLGVKAVIAKSFERIHA
                     SNLVNVGILPLVFRDEGDYN"
     gene            2174..3244
                     /gene="ychF"
                     /locus_tag="P5573_05910"
     CDS             2174..3244
                     /gene="ychF"
                     /locus_tag="P5573_05910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HRT27630.1"
                     /translation="MKIGFIGLPNSGKTTIFNALTKSKAEITAYANTRGEPNLAVVEV
                     IDERVTRLAEIYRPKKTTYAAIEFIDFAGLSEGSGKGDAFSGTAMGLIKGVDALALVI
                     RNYPDDLMGMPSPLQDMDQIDLEMLVSDLAIVEKRLERIARFYQRGQKTEALQTEEKV
                     LRKISAHLNGNQPVRTMELNGEEEKVIRGFQFLTKKPLMVILNSHESNFGRNEDLLEE
                     IGKKHGAIEFAGHFEMELSRLEDKAEALMFMKDMGIERSARDRLTQFAYSLLGYISFF
                     TVGADEVRAWTIRRGETAVEAAGAIHSDLSKGFIRAECFSYNDLLECGSEKGVREKGR
                     FRLEGKNYVVKDLDILSIRFNL"
BASE COUNT          718 a         1001 c          896 g          729 t
ORIGIN      
        1 gttgtagtcg ccttcgtccc ggaaaaccag cggcaggatg ccgacgttga cgaggttcga
       61 ggcatgaatc cgctcgaatg atttcgcgat gacggctttc accccaagat acatcgggca
      121 aagggctgcg tgttcccgcg acgaaccctg gccgtaagaa aggcccgcca cgatgatgtt
      181 atgccgccct tcttccttta ttttcgccgc cctgccggcg aaacccgggt cgaccgattc
      241 gaacacgaac tcggaatatt tcgggatgtt cgacctgtat ttcatccttg ccccggcagg
      301 aattatatgg tctgtcgtta tcttatcccc cacaactatc gtgacctcgc cgtcgatccg
      361 gtcgggaagc ggctcgttga ccggaggggt cccgatattc ggccccctgt aaacatctct
      421 gaaacggctt tttcccgcct cttccggcct gatgatcatc ctgtcgtcga cgataaaagt
      481 ctccggcatc tccaccttcg ggtagggaat gccgagttcc ctcgggtcgg ttatccgtcc
      541 cgtgatcacg gccgccgccg ccgtttcggc gctgaccagg tacacccgcg cgtctttcgt
      601 ccccgagcgg cccataaaat tcctgttcga tgtccgcaga gaaacgcccc ccgacatcgg
      661 gctctggctg ttgccgatgc aaaaaccgca ggcgctctcc atgatccggg cccccgctgc
      721 ggctagaatt tcaaggtaac cctgccgggc gaggttaaga agaacctgcc tcgacccggt
      781 cgaaagcaca aaactgacat caggatgaac cgtctttcct ctgagcatct ccgccacgat
      841 cgcgaggtcg cggtaagagg cgttcgtaca gctcccgata cagacctggt ccaccggaac
      901 gcccgcaatg tctttaacgg cggcgatgtt gtccgggctg tggggcaagg cggcaagcgg
      961 gacgaggcga gaaaggtcga tgtcgacgac acgctcatag actgcgtcac catccggttc
     1021 caaccccctc cagtccttct cccggccctg ggacctcaag aataccctcg tgacctcatc
     1081 gctcggaaag atggaggtcg tgaccccgca ttcggcgccc atgtttgcga tcgttgccct
     1141 ctcggggaca gatagaccct ctacgccttc gccggtgtac tcgaaaaccg tgttgacgtt
     1201 gcctttcgtc gtgaaaatct ccaacacctt gagaattacg tccttcgcag acacccaggg
     1261 ttggagcttt ccagccaacc tcacctggac gacccggggg cagataagat aaaagggcct
     1321 tccgcccatc gcgagggcta catcgagccc tcccgcaccg atggcgatca tccccagccc
     1381 accgcccgtc ggggtatggc tgtccgcgcc aaggagcgtc tttcccggtt taccgaacct
     1441 ctctaaatgc acttggtggc agatcccgtt cccggcacgg gaaaggtaaa ttccgtactt
     1501 tgtcgcgaca ctctggaggt aacgatggtc gtcggcgttc tcaaagccga cctggatggt
     1561 gttgtggtcg atgtagctga ccgacaactc cgtcgccacc ctttccaatc ccatcgcctc
     1621 gaactgaaga taggccatcg tgccggtggc atcctgggta agggtctggt cgatccgtat
     1681 cccgatctca gttcccggct cgaatcgacc tgagataaga tggtcctcga tgatcttctg
     1741 tgtcagcgtc ctgcccatcg gggcctccgt ttccgcttac gcctgcaaaa tttgtagtgt
     1801 cttcagacat ctcttcttcc cccagccttc actgcatcca taccacgaca agcatccttc
     1861 aaaagaaccc gcaagtgaac gatttcattt taaggaaaaa gatatcccct gtaaagaaga
     1921 atcccggggc acattaaatt tagtcttgac ttttcaccgg cctcatatta agagcagcac
     1981 cgtttttgcg cccgccgggc gccgtaaaaa aagaaccgca gatttgaagt caaaggtggg
     2041 ccgctgtgct cttgagcttc ggtggccttt ttcttttcgc cccccgtcat tgcgatgcgc
     2101 cccctggcgg agaagcaaat ctcggttctg cttccggcga ctttatcgtt tcatgaagag
     2161 gagcttttga ccaatgaaaa tcggtttcat cggcctgccg aattccggca agaccaccat
     2221 attcaatgcc ctgaccaaat cgaaggcgga aatcacggcc tatgccaaca cccgcggcga
     2281 accgaacctc gccgtcgtgg aagtgatcga tgagcgggtg acaaggctgg cggaaattta
     2341 ccggccgaaa aagacgacct atgccgccat tgagttcatc gactttgcgg gtctgtcgga
     2401 agggtccggc aagggagacg ccttctccgg gacggccatg ggactgatca aaggggtcga
     2461 cgccctggcc ctagtgatcc gcaactatcc ggacgatctg atggggatgc cttcacccct
     2521 ccaggacatg gatcagatcg atctggaaat gctcgtctcc gacctcgcca tcgtggagaa
     2581 gcggctggag cggatcgcgc ggttttacca gcgcggccag aagacagagg ccctgcagac
     2641 ggaagagaag gtcctgcgca aaatatcagc ccacctgaac ggcaaccagc cggtccggac
     2701 catggaactg aacggtgagg aagaaaaggt catccggggt tttcagttcc taacgaaaaa
     2761 acccctgatg gtcatcctca attctcatga aagcaatttc ggccgcaatg aagacctcct
     2821 ggaggaaatc gggaagaagc atggggccat tgaatttgcc ggccattttg aaatggagct
     2881 ctcccgcctg gaggacaaag cggaggccct gatgtttatg aaagacatgg ggattgaacg
     2941 ctccgcccgg gacaggctga cccagttcgc ctattccctt ttggggtaca tcagtttttt
     3001 caccgtcggg gcagacgagg tgcgcgcctg gacgattcgg aggggcgaaa ccgccgtgga
     3061 ggccgctggc gccatccatt ccgacctttc caaggggttt atccgcgccg agtgtttttc
     3121 ctataatgac ctcttggaat gcggctcgga gaagggggtc cgcgagaaag gccgcttccg
     3181 gctggaggga aagaattacg tggtcaagga tcttgatata ctcagtatcc gcttcaacct
     3241 gtgacagacg gcttaaaaat gctcatcttt gggacgattg attcatcatt cgtcccacat
     3301 catcttgtcc ttgcccaccg tcgaaaaaca ttatacattc cctc
//
LOCUS       DAOTMX010000181         3339 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_22488_length_3339_cov_2.711023, whole genome shotgun sequence.
ACCESSION   DAOTMX010000181 DAOTMX010000000
VERSION     DAOTMX010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22488_length_3339_cov_2.711023"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1809
                     /locus_tag="P5573_05915"
     CDS             <1..1809
                     /locus_tag="P5573_05915"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HRT27631.1"
                     /translation="MIGGMADPDSVKAALDIFHDSFNRLAELKFPTVAAINGVCAGGG
                     LEFALACTARIATDAKNTLIGLPECKVGLFPGGGGSQRLPRLIGYPAVELILKGELLP
                     AAKALEAGIIDRLVPAGGNLVEEAKAFLKDIAAGKAQLKRPHHDFSQIDMVAEKARQG
                     VLQMTRGRELPGPMFGIKAMQEGLKVSLKEGLEIEKKYFIQTVLSPEAKGSINTFFLK
                     TMTDKPRRMMRQGLTPAAVRKAAVLGFGTMGRGIVIDIVRNMRIPVLVKDFPETHEPG
                     KAFVRKILEGMAVKRKLPAPVDDLMGLIKPLSEYTPEFKEADLVVEAVFEDIRVKEQT
                     YRELCPQVRDDCLIASNTSSLLVTALSKFLTHPERFGGMHFFSPVWMMELVEVVRGEA
                     TSRATVDNLLNFAAAIRKRPIVCRDNPGFVVNALLLPYLLGAFRHLEAGNPIEKIDEA
                     MTRFGMPVGPIRLTDEVGIDVPYKVLTGMGIEQDTLKNVVEAGRLGLKKSGKGFFLKD
                     GTVDPEVLPLIALKEPKEATAEEIQTDALVAMVRVGKDLLDRHVVDDPRMIDVGMIWG
                     IGFPPDKGGPMKWADIIGLSKKLFGENFYQPSAVGS"
     gene            complement(1856..2356)
                     /locus_tag="P5573_05920"
     CDS             complement(1856..2356)
                     /locus_tag="P5573_05920"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HRT27632.1"
                     /translation="MKKTLTIITMLLFLAVAVSPSLAARGRGMGPGEPGGGGCIDPTV
                     LKELNLTAEQKAKISALREAHLKEVKPLHDKLFSLKGDLRLLWLEKNPDRGKIAAKRK
                     EIRGLQDQLQDKRDAYFLEVLNVLTPEQKESFRAACPGGGHGPEMWHGRDYGHGPGGP
                     GMRGDW"
     gene            2718..>3339
                     /locus_tag="P5573_05925"
     CDS             2718..>3339
                     /locus_tag="P5573_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HRT27633.1"
                     /translation="MIIGSGPIVIGQACEFDYSGTQACKALRKLGYEIVLVNSNPATI
                     MTDPGMADATYIEPLNLQTMVEIIENERPDAILPNLGGQTGLNLTSELARSGILEKYG
                     VKVIGVQVDAIERGEDRIAFKETMRRLGIEMPKSEPAYTVEEAEKIAAALGYPVVIRP
                     AYTMGGTGGGLVYNVEELRTVASRGIAASLVGQILVEESVLGWEELE"
BASE COUNT          725 a          955 c         1005 g          654 t
ORIGIN      
        1 atgatcggag ggatggcaga ccccgactcc gtgaaggcgg ccctggatat cttccatgat
       61 tccttcaacc gcttggcaga gctgaaattc cccaccgttg cggcgataaa cggcgtttgt
      121 gccggaggcg ggctggagtt cgcactggcc tgcacggcaa ggatagccac cgacgcgaag
      181 aacacgctca tcggcctgcc ggagtgtaag gtgggtcttt tccccggcgg gggaggcagc
      241 caacggttac cccgcttgat cggctacccg gccgtggaat tgatcttgaa aggcgaactt
      301 ctgccggcgg cgaaggccct cgaggcgggc atcatcgata ggttggtacc ggcgggcggt
      361 aacctcgtcg aggaggcgaa ggcctttctc aaggacatcg ccgcgggaaa agcacaactg
      421 aaaagaccgc accatgattt ctcccagatc gacatggtcg ccgaaaaggc ccgccaaggt
      481 gtcctccaga tgacaagggg tcgcgaactc cccggtccga tgttcggcat caaagccatg
      541 caagaggggc tcaaggtgtc tctaaaagag gggttggaga tcgaaaaaaa gtatttcatc
      601 cagaccgtcc tctccccgga ggcgaagggc agcataaaca cattctttct caagacgatg
      661 acggacaaac cccggcgcat gatgagacag ggcttaaccc cggcggcggt caggaaagcc
      721 gcggtgctcg gcttcggaac gatgggccgg gggatcgtga tcgacatcgt gaggaacatg
      781 cggatcccgg tcctcgtgaa ggattttccg gagacccatg aaccggggaa ggcattcgtc
      841 agaaaaatcc tggaagggat ggcagtcaaa agaaagcttc cggcaccggt cgacgacctc
      901 atgggcctga tcaaacccct gtccgaatac acgccggaat tcaaagaggc ggacctggtt
      961 gtagaagcgg tgttcgagga catcagggtg aaagagcaga cttaccggga actctgcccg
     1021 caggtgcggg acgactgcct gatcgccagc aacacctcgt cactcctcgt caccgccctg
     1081 tcgaagttcc tgacccaccc ggagaggttc ggcgggatgc atttcttttc gccggtctgg
     1141 atgatggagc tcgtggaggt cgtgagaggt gaggcaacga gcagggcaac ggtagacaac
     1201 ctgctgaatt tcgccgccgc gatccgcaag cggccgatcg tctgccggga caacccaggg
     1261 tttgtcgtca acgcccttct attgccgtac ctcctgggtg cctttcgcca cctcgaggcg
     1321 ggtaacccca tcgagaaaat cgacgaggcc atgacccgct tcgggatgcc cgtcggtcct
     1381 atccgcctca ccgatgaggt ggggatagac gtcccctaca aggtgctgac cgggatgggc
     1441 atcgagcagg ataccttgaa gaatgtggtg gaggcggggc gtctcggtct caaaaaatcc
     1501 gggaagggct tcttcctgaa agacgggacg gtcgacccgg aagtcctacc ccttatcgct
     1561 cttaaggaac cgaaggaggc tacggccgag gaaatccaga cggacgccct cgtcgccatg
     1621 gttcgggtcg gcaaagacct cctggaccgg cacgtcgtcg acgacccccg aatgatcgat
     1681 gtgggtatga tctgggggat aggctttccc cccgacaaag gcgggccgat gaaatgggcc
     1741 gacatcatcg gtttgagcaa gaagctcttc ggggagaatt tttatcagcc gtcagcggtc
     1801 ggctcctgag gggggatata tttgccgacc gccgacgggt ttacgcccac tgggcttacc
     1861 agtcgcccct catacccggc ccgccggggc cgtggccgta gtctctgccg tgccacatct
     1921 ccggcccgtg acctccaccc gggcaggcgg cccggaacga ctctttctgt tccggggtga
     1981 ggacattgag cacctcgagg aagtaagcgt ctctcttgtc ctgaagctga tcctgcagcc
     2041 ctcttatctc tttccgcttg gcggcgatct ttccccggtc gggatttttc tcgagccaga
     2101 gcagcctcag gtcgcccttc aggctgaaca gcttgtcgtg caagggcttg acttccttca
     2161 ggtgcgcttc ccggagggcg gagatcttag ccttctgctc cgccgtcagg ttgagttctt
     2221 tcaagacggt cgggtctata cagcccccgc cgcccggctc gccgggtccc atcccccgtc
     2281 ccctggcggc gagggacgga gaaaccgcga cggcaagaaa cagaagcatg gtaatgattg
     2341 tcaatgtctt tttcatggtt ttttgctcct ttcgtgttct cttggtttta gactctctac
     2401 ggcaaaggac atgccagaca aagggccgcg cataactagc tgcaatcatt gataacaatt
     2461 gaatgatttt gtcgaactcc atgaccttcg agggcaaagt ggataccaag tatccgaccg
     2521 aggcctcgac ctgcattata attatccacc atgcgcgaat cgcgcttttt ctcccttgac
     2581 ttccccggca tacgtcccta tagtttgccc gctctgccac gtcgttatgc tattgttttt
     2641 tgaatgaata cggatttgtc ccaagtaatg aaagggaact cagtgtcatg ccgaaaagga
     2701 aagatataaa aaaagtgatg ataatcgggt cgggccccat cgttatcggg caggcctgcg
     2761 aattcgatta ctccggcacc caggcctgca aggccttgag gaagttgggc tatgagatcg
     2821 tcctcgtcaa ctcgaacccg gccactatca tgaccgaccc cgggatggcc gatgcaacct
     2881 acatagaacc gctgaacctc cagaccatgg tcgaaataat cgagaacgag aggccggacg
     2941 ccatcctccc caacctgggg gggcagacgg ggctcaacct cacctcggag ctcgcccgta
     3001 gtggaatcct cgagaaatac ggcgtgaagg tcatcggcgt ccaggtcgac gctatcgaac
     3061 ggggtgaaga ccggatcgcc ttcaaggaga ccatgaggcg cctcgggatc gagatgccga
     3121 aaagcgaacc agcatacacg gtcgaagaag ccgaaaagat cgcggctgca ctgggttacc
     3181 ccgtagtcat ccggcctgcc tacacgatgg gagggacggg gggcggcctt gtttacaacg
     3241 tggaagagct gcggacggtg gcaagccgcg gcatagcggc gagcctcgta gggcagatac
     3301 ttgtcgaaga gtccgtcctc ggctgggaag agcttgagc
//
LOCUS       DAOTMX010000182         3336 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_22513_length_3336_cov_3.523926, whole genome shotgun sequence.
ACCESSION   DAOTMX010000182 DAOTMX010000000
VERSION     DAOTMX010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22513_length_3336_cov_3.523926"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..750)
                     /gene="pgsA"
                     /locus_tag="P5573_05930"
     CDS             complement(139..750)
                     /gene="pgsA"
                     /locus_tag="P5573_05930"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HRT27634.1"
                     /translation="MVIDFLKKSFDRLPIPIEGREIVNLPNCITMLRIAVIPILFLIL
                     LDPGQKLSLVIAILFVLAALTDLLDGYIARKYEIVTKTGKLLDPIADKLIISTAMILM
                     IPIGRIPAWVVALIVMRDFVVDGVRQVASSGGVVISASRLAKQKTVSQVAAVTALLIH
                     YPFLGMDAHRVGILLLYLSLVLTVWSGIDYLIRFYRETVRAGL"
     gene            complement(757..900)
                     /locus_tag="P5573_05935"
     CDS             complement(757..900)
                     /locus_tag="P5573_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27635.1"
                     /translation="MEIVAPIRIIAAIPFNIIYQSAKRQMTGVGTGEVYERLGKECRM
                     SEV"
     gene            complement(951..1241)
                     /locus_tag="P5573_05940"
     CDS             complement(951..1241)
                     /locus_tag="P5573_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27636.1"
                     /translation="MLTRVILVVFVLYLLYRLLKSIMTPSVWKDPSSEKRPEISEELV
                     EDPFCHTLVPIGAAFKAVIAGKTVYFCSPDCYEKQITAEKSGSSMTDDRKMK"
     gene            complement(1251..1760)
                     /gene="folK"
                     /locus_tag="P5573_05945"
     CDS             complement(1251..1760)
                     /gene="folK"
                     /locus_tag="P5573_05945"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HRT27637.1"
                     /translation="MNRSFRSVIAYLGIGSNLDHPVMQCRRAVERISALDIGVLRKSS
                     LYQTEPVGYLGQDWFVNAAVEIRTTLRPRSLLGSLQELEKSLGRVRTVKGGPRSIDID
                     ILLYDREIIDEEGLSIPHPELHRRRFVLVPLAEIASYVIHPVFGISVRGLLDRLEDTK
                     KVIRLEEAL"
     gene            complement(1741..2862)
                     /locus_tag="P5573_05950"
     CDS             complement(1741..2862)
                     /locus_tag="P5573_05950"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HRT27638.1"
                     /translation="MELRKKIAQAKADGIDVISLAIGDPVEPTPESVVEELCRRARDP
                     ENHRYPTDEEKGMMAFRKEVARWYGDRYKVLLDPEREVLGLIGSKEGCHHFVLARINP
                     GDIVLMTDPGYPAYRASIIMAGGEPYSVPILRENGYLPRLEDVPSDVARRARGMFLNY
                     PNNPTGACADRPFFRDLVAFAKDYDIAVCYDNPYSEIVFDGQERLSFLSVEGAKDVGV
                     ELNSLSKPFNMTGWRIGMAVGSPDLIAAISKVKENTDSGIFNPIQYAAIKALRDESKN
                     IDRMVEIYSRRRALVLQTLDRIDIRFEPPKGTFYLWVPTPEGMSALEFTGKLFERAAV
                     VVAAGTAYGRYGEGYIRISLTVPDNRLQEAMERIEQEFS"
     gene            complement(3013..>3336)
                     /gene="dapB"
                     /locus_tag="P5573_05955"
     CDS             complement(3013..>3336)
                     /gene="dapB"
                     /locus_tag="P5573_05955"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HRT27639.1"
                     /translation="PSGTAMRMAEIIAGALGRDLQKVGVYERKGMIGARTKEEIGIQT
                     IRAGDIVGEHTVIFGGIGERLEFIHRAHSRDNFAKGALRAAKWVVGQKNGFYDMQDVL
                     GLKDK"
BASE COUNT          756 a          961 c          818 g          801 t
ORIGIN      
        1 ccgtcgacgt ctaccttggc aaggtagcac tctaccgctg agttacgccc gctcaatcgg
       61 agttatttga ataataaaat cggcttgaat tgtcaacaga aaattaaata ttccagatga
      121 aaatctttca cgatagaatc acagaccggc tctaaccgtc tcccggtaaa accggatgag
      181 ataatcgatg ccggaccaga ccgtgaggac aagagaaaga taaaggagga gtatgcccac
      241 cctgtgcgcg tccatcccaa gaaaaggata gtgaataagg agcgcggtta cagcggccac
      301 ctgggacacc gtcttctgtt ttgcgagcct gcttgcagaa atcactacgc ccccggatga
      361 ggcaacctgg cgcactccgt cgacgacgaa atcccgcatg acgatcaagg ccacgaccca
      421 tgccgggatt cttccgatcg gtatcatcag tatcatggct gtgctgatga tgagtttgtc
      481 tgctatcggg tcgaggagtt ttcctgtctt ggtaacgatc tcatatttcc gtgcgatgta
      541 cccgtcgaga aggtctgtca acgcggcgag aacgaatagg atcgcaataa ccaagcttag
      601 tttttggccc gggtcgagta aaatgagaaa tagaatcgga atgacggcta tcctcagcat
      661 tgtgatacag ttcggcaggt ttacgatttc ccggccctca ataggtatag gcaaccggtc
      721 aaaagatttt ttcaagaaat ctatcaccat gcttcctcaa acctcactca tacgacactc
      781 ttttcccagt ctctcataaa cttccccagt cccgacgccc gtcatctggc gcttggccga
      841 ttgatagatt atgttgaaag ggatggccgc gattatccga atcggggcaa cgatttccat
      901 tccgacttgg gtgaaatcaa ccaaccggct cacaaggagc tgatgatatt ttacttcatt
      961 tttctgtcgt cagtcatcga tgagcccgat ttctccgcag taatctgttt ttcataacag
     1021 tccgggctgc agaagtaaac cgtctttccg gcaataacag ccttgaaggc ggccccgatc
     1081 gggacgaggg tgtggcagaa ggggtcttca acaagctcct cgcttatttc aggccttttt
     1141 tcgctcgaag ggtctttcca gaccgacggg gtcatgatgc ttttcaacaa tcggtaaagc
     1201 agatacaaga caaaaacaac cagaatcact cgggtcagca taaagaatcc ttacagggcc
     1261 tcctcgagcc ggatgacttt ttttgtgtct tcgagcctgt cgagaagtcc cctcacggag
     1321 ataccaaaga cggggtggat gacataggac gcaatctccg cgagaggaac caacacaaaa
     1381 cgccgcctat ggagttcagg atgcgggatc gagagccctt cttcgtcgat gatttccctg
     1441 tcgtagagca ggatatcgat gtctatcgac cgtgggcccc ctttaactgt cctcaccctg
     1501 cccaaagatt tctccagttc ctgcaaggag ccgaggaggc ttcgaggcct cagcgtggtc
     1561 ctgatttcca ccgccgcatt tacaaaccag tcctgcccca aataaccgac cggctccgtc
     1621 tgatagagag acgactttct caaaacgccg atgtcgagcg cagagatgcg ttcaaccgcc
     1681 cttcggcact gcattacggg atgatccagg ttcgagccga tcccgaggta tgcgatgacg
     1741 ctacgaaaac tcctgttcaa tcctttccat tgcctcttgc agcctgttgt ccgggaccgt
     1801 aagggaaatc ctgatataac cctcgccgta ccttccgtag gccgtccctg ctgcgaccac
     1861 aacggccgct ctttcgaata gcttaccggt aaattccagt gcgctcattc cttccggggt
     1921 cggaacccag aggtagaatg ttccttttgg cggctcgaac cgaatgtcta tcctatccag
     1981 ggtttgcaga acaagggctc tgcgccgaga atagatttcg accatcctgt ctatgttttt
     2041 cgactcgtcc ctcaacgcct ttatggcggc atactgaatg ggattgaaga tgccggaatc
     2101 ggtgttttcc ttaaccttac tgatagccgc gatgaggtcg ggacttccca ccgccatgcc
     2161 gatccgccag cccgtcatgt taaaaggctt cgaaagggaa ttgagctcga cgccgacgtc
     2221 cttcgccccc tcgacactca ggaaactcag tcgttcctgg ccgtcaaaga caatctcgct
     2281 gtagggattg tcatagcaga cggcgatgtc gtagtccttg gcaaaggcga ccaggtcgcg
     2341 gaaaaaaggc ctgtcggcac aggcccctgt ggggttattc gggtagttca gaaacattcc
     2401 gcgggccctt ctggccacgt ccgaggggac atcttccagc ctcggcaggt acccgttctc
     2461 ccgcaagata ggcaccgaat atggttctcc cccggccatg attatgcttg ccctgtaggc
     2521 cggataaccc gggtcggtca taagaacgat gtccccgggg ttgattcttg cgagaacgaa
     2581 gtggtggcaa ccctctttag agccgatcag accaaggact tcccgctccg ggtcaagcaa
     2641 taccttatac ctatcgccgt accatcgggc gacctccttc cggaacgcca tcatcccttt
     2701 ttcctcgtcg gtagggtacc gatggttctc ggggtccctc gctctccggc atagttcctc
     2761 gacgaccgac tcaggcgtcg gctctaccgg gtcccctatc gccaggctga tcacgtcgat
     2821 gccgtcggcc ttcgcctggg cgatcttctt cctcagctcc ataaaaaggt aaggtggaat
     2881 tttcgtcaat ctgtctgcaa cctgcaagat gttcctcctc cggtctattc cagcttcagc
     2941 ggtcaactcc tgaccgaaat gaaacgacaa tcctatccaa tctgtccttg tatatttcaa
     3001 tcacgcaaga cgttatttgt ctttgaggcc gaggacatcc tgcatatcgt agaaaccgtt
     3061 cttctggcct acaacccact tggcggcgcg gagggcaccc ttggcgaaat tatccctgct
     3121 gtgcgccctg tggatgaatt cgaggcgttc cccgataccg ccgaaaatga ccgtgtgctc
     3181 gcccacgata tctcccgccc ttatggtctg aataccgatt tcctccttcg tcctggcgcc
     3241 gatcatccct tttcgttcgt aaaccccgac cttttgcaaa tcccttccca gggcaccggc
     3301 gatgatctcg gccattctca ttgccgtgcc gctcgg
//
LOCUS       DAOTMX010000183         3326 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_22605_length_3326_cov_3.670743, whole genome shotgun sequence.
ACCESSION   DAOTMX010000183 DAOTMX010000000
VERSION     DAOTMX010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22605_length_3326_cov_3.670743"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2027
                     /gene="mfd"
                     /locus_tag="P5573_05960"
     CDS             <1..2027
                     /gene="mfd"
                     /locus_tag="P5573_05960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HRT27640.1"
                     /translation="EGYFLQSFGELKEGDFIVHADHGIGIYRGLEKLAVGGIENDFLN
                     LEYSEGDKLYIPVDRLDRIQRYIGPDGHDPKVDKLGGTSWENAKKRVKRSVEEVAEEL
                     VSLYAARETLERLPFAPTDAYYDEFCSSFEFEETPDQARAIEDVNEDMADSKPMDRLV
                     CGDVGFGKTEVAIRAAFRAVMEGKQAAVLVPTTILAEQHYQTFSRRLEKYPIRIDVLN
                     RFKTRNEQQKVIEDLGKGKIDIVIGTHRLLQKDVSFKELGLVVVDEEQRFGVSHKEKL
                     KQLRTLVDVLTLTATPIPRTLQLSLVGIRDLSIINTPPEDRRSIKTHVVEFDEEVIKN
                     AVRSELGRKGQVFFVHDRINSIYTMERLVRRLVPEARVAVAHGRMKSRELEDVMVGYV
                     RRDYDVLVCTTIVGSGIDIPSANTIIINRADRFGLSQLYQLRGRVGRSREEAYAYLLI
                     PRGAMLAPDTQKRLRVIRELTEPGSGFAVASHDLEIRGAGNILGTSQSGHVAAVGYEM
                     YIQLIENTIRELKGEAAPEEEVRPEIHLGLPAFIGEDYMPDMNRRLVMYKRISMASTE
                     EDISALREELLDCYGHIPVQVENLLEVIRIRNLAKPIAAKRIDYDGKNLSVSFMKSSS
                     LEPEKILKLIRKKTKEVKFTPDYRLIIPIPGLQHSEVIGEAKGLLRELAD"
     gene            2085..3062
                     /locus_tag="P5573_05965"
     CDS             2085..3062
                     /locus_tag="P5573_05965"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HRT27641.1"
                     /translation="MQRRHFHINAALIAAFVVLATTGCQFFGGSQDYVATVDGEKIPL
                     EDFNDRLEQKLALMEGRSSMSDEQFEVLKQNVLNELIDEQVMLNRARKLGLSVSEGEL
                     KRRIEEIRKDYGEEHFEQYFKGGRKEYRTWEEKLRRRLLLEELIRQEVNSRVSVTDEE
                     VMDYLKKHEKEKLFQERVRLSQIVFQDREKAEMVMQRLNDGEDFATLAREFSLGPEAE
                     KGGDLGYFVRGVLPETFDRVIFSLPPGKVSGIIETPYGYHIFKVLEKERRGKKVLAEQ
                     RERIRAKLKREKEDQEYAGWLERLRSESVIKINEPVLRRVGREGGRAER"
     gene            3101..>3326
                     /locus_tag="P5573_05970"
     CDS             3101..>3326
                     /locus_tag="P5573_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27642.1"
                     /translation="MNRIVVIIVSILTLFFSLEAGRADVVDRVVAVVGNDVITLYELD
                     NIVKTYIERMGKSLGPEDHNRVVEETRKVVL"
BASE COUNT          830 a          820 c         1067 g          609 t
ORIGIN      
        1 atgagggcta cttcctccag tccttcggcg agctcaagga gggtgatttc atcgttcatg
       61 ccgaccacgg catcggcatc taccggggac tcgagaagct cgcagtggga ggtatcgaga
      121 acgattttct caacctggag tactcggagg gcgacaagct ctacatcccc gtcgaccgtc
      181 tcgaccggat acagaggtac atcggccccg acgggcatga cccgaaggtg gacaagctcg
      241 gagggacgtc ctgggagaac gcaaagaaac gggtgaagcg ctccgtcgaa gaggtcgccg
      301 aagagctcgt ttccctctat gccgcgaggg agaccctcga acgcctgccc ttcgcgccca
      361 ccgacgccta ttatgacgaa ttctgctctt ccttcgaatt cgaagagaca ccggaccagg
      421 cgagggccat cgaagacgtc aacgaagata tggccgactc aaagccgatg gacaggctgg
      481 tgtgcggcga cgtgggtttc ggcaagacgg aggtggcaat aagagcggct ttccgcgccg
      541 tgatggaagg gaaacaggcg gccgtgttgg tgccgacgac gatcctggcc gaacagcatt
      601 accagacctt ttcgcggcgg ctggagaaat atcccatacg catcgacgtg ctgaaccgct
      661 tcaagacccg gaacgaacag cagaaggtca tcgaggatct tggtaaagga aagatagaca
      721 tcgtgatcgg gacgcaccgg ctcctgcaga aggatgtttc cttcaaggaa ctcggcctgg
      781 tggtcgtgga cgaagagcag cgcttcgggg tcagtcacaa agaaaagctc aaacaactgc
      841 ggactctcgt ggacgtcctg accctgacgg cgacgccgat cccgaggacg ttgcagcttt
      901 ctctggtggg tatccgcgac ctatccataa tcaatacgcc cccggaggac aggcgatcca
      961 tcaagaccca cgtcgtggag ttcgacgagg aagtgatcaa gaacgccgtc cgttccgagc
     1021 tggggaggaa gggccaggta ttcttcgtcc acgacagaat caattccatc tacacgatgg
     1081 aaaggctcgt caggcggctg gtgccggagg ccagggtggc ggtggcgcac ggacgcatga
     1141 agagccggga attagaagac gtcatggtgg ggtacgtccg ccgcgattat gacgtccttg
     1201 tctgcacgac gatcgtcggt tcgggcatcg acatcccttc ggccaacacg atcatcatca
     1261 accgggcgga ccgtttcggc ctttcccaac tctaccaact ccgggggagg gtgggcaggt
     1321 cgagggagga ggcctatgcc tatctcctca tccccagggg tgcgatgctc gccccggaca
     1381 cgcaaaagag gttgcgggtg atccgggagc tcacggaacc aggttcaggc ttcgccgtcg
     1441 cctcccatga cctggagatc aggggtgcgg ggaatatcct ggggacttct cagtcgggcc
     1501 acgttgcggc cgtgggctat gagatgtaca tccagcttat cgagaacacg atcagggagt
     1561 tgaaagggga ggcggcgccg gaagaggagg tcaggcccga gatacatctc ggactaccgg
     1621 ccttcatcgg cgaggattat atgcccgaca tgaaccgaag actcgtgatg tacaagcgga
     1681 tatccatggc ctctacggag gaagacatat ccgccctacg ggaagagctt ttggactgtt
     1741 atgggcacat accggtgcag gtcgagaatc ttttagaggt gattcgcatc cggaatcttg
     1801 cgaagcccat tgcggccaag cggattgact acgacggcaa gaatctctct gtttccttca
     1861 tgaagagcag ctccctggaa ccagaaaaga tattgaagct catcaggaag aaaacgaaag
     1921 aggtgaaatt cacacccgat tacaggctca tcatccccat ccccggcctg cagcatagcg
     1981 aggtaatcgg cgaggcgaag gggctgctga gggaattggc ggattaacaa aaggaatcga
     2041 gaaggcgatg gttcggagga agggggacgc ggggaaaaaa ggcgatgcag agaagacatt
     2101 tccacataaa cgccgctttg attgcggcct ttgtggtgct ggcgacgacg ggttgtcagt
     2161 tcttcggggg ttcgcaggac tacgtggcga cagtcgacgg cgaaaagatt cctctggagg
     2221 acttcaacga ccggctggag cagaaactcg cgctcatgga aggcaggtct tcgatgagcg
     2281 acgagcaatt cgaagtcttg aaacagaacg tcctgaacga gctcatcgac gagcaggtca
     2341 tgctgaaccg ggcgaggaaa ctcggtctct ccgtcagcga aggggagctc aagagacgta
     2401 tcgaagagat caggaaagat tacggagaag aacattttga gcagtatttc aagggaggcc
     2461 ggaaggaata ccgaacgtgg gaagagaaac tgcggcggag gcttctcctg gaggagctca
     2521 tccggcagga agtgaactcc cgggtgtcgg tgacggatga agaagtcatg gattatttga
     2581 agaaacacga gaaggaaaag ttgttccaag aacgggtccg cctcagccag atagtatttc
     2641 aggacaggga aaaagccgag atggtcatgc aaaggttgaa cgacggcgaa gattttgcga
     2701 ccctggcgag agagttttcc ctggggcctg aggccgagaa agggggcgac ctcggctatt
     2761 tcgtgcgggg cgtcttgccc gagacctttg acagagtcat cttctctttg cccccgggga
     2821 aggtgagcgg gatcatcgag acgccgtacg ggtatcacat cttcaaggtt ctcgaaaagg
     2881 agcgaagagg caagaaagta ttggccgagc aaagagagcg gatcagggct aagctgaaaa
     2941 gagagaaaga ggaccaggaa tacgccggat ggctggagcg attgaggagc gagtcggtca
     3001 taaagataaa cgaaccggtg ctcaggcgcg tcggccgcga gggcggaagg gcggagagat
     3061 aataatctcc aggtatcgaa taagagaaga ggaaggggca atgaaccgta tcgtcgtcat
     3121 catcgtttca atcttgacgt tgtttttttc cttggaggcc ggccgtgccg atgtcgtcga
     3181 ccgagtggtc gccgtggtgg gtaatgacgt catcacgctt tacgagctgg acaacatagt
     3241 aaagacgtac atagagagga tggggaaatc tctcgggccc gaggaccata atagggtcgt
     3301 ggaggagacg cggaaggtcg tactca
//
LOCUS       DAOTMX010000184         3311 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_22761_length_3311_cov_4.231265, whole genome shotgun sequence.
ACCESSION   DAOTMX010000184 DAOTMX010000000
VERSION     DAOTMX010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22761_length_3311_cov_4.231265"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="P5573_05975"
     CDS             complement(<1..229)
                     /locus_tag="P5573_05975"
                     /inference="COORDINATES: protein motif:HMM:NF014635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HRT27643.1"
                     /translation="MKEWQDLGRQLKGYINVDTFPVAIRFLKNKEDMPEGTRTPLKHM
                     KVKMAHCQVQTVARKYGWTIAMTKDDLGCAIS"
     gene            complement(234..671)
                     /locus_tag="P5573_05980"
     CDS             complement(234..671)
                     /locus_tag="P5573_05980"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRT27644.1"
                     /translation="MGNDDRLIYLLSIAQQALKDHANRSISEAGGKVTLAQAAILFLL
                     KAKDRRYMSEMGQIIGVDNSAMTRLVDRLEKAGLVNRQIDPQNRRAISILLTPAGLKE
                     AAAALTVIKRINEEIKKDYSPAEVEAFKKVLGGILRKFKTPKE"
     gene            842..1036
                     /locus_tag="P5573_05985"
     CDS             842..1036
                     /locus_tag="P5573_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27645.1"
                     /translation="MAMRLIRESGRNVSLLLQDFKAEFLKRAEGDVFFTCEDGPAIRA
                     LVEKAMTTDDRVNSPVRVTL"
     regulatory      1249..1338
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1404..1760
                     /locus_tag="P5573_05990"
     CDS             1404..1760
                     /locus_tag="P5573_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27646.1"
                     /translation="MRGAEDYSTDWRYTSLGSTAGRQTAKKDISIVGVVGDLLFITGL
                     IFLGFEIFFWVRHGEWLGYPCTLILDAVPSAFLASLENSPVLKGIVVGALKRLDFSVV
                     LVISGYILGRKFGDLL"
     gene            complement(1815..2825)
                     /locus_tag="P5573_05995"
     CDS             complement(1815..2825)
                     /locus_tag="P5573_05995"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRT27647.1"
                     /translation="MKRQPKRFCSLCGGVTVTRREDNVPRSYCETCGVFFYENPLPVV
                     STILLSDRKILLVKRGKKPYRGMWCLPTGFAEAGESIEDAALRELEEETGMRGSIVGL
                     VDLGSAENYYYGDLLFVTFEVEATGGRLARGGDADTVRYFPLDRIPRLAFTSNKKAIE
                     AFIRSKADAWAILDSFNLAVTEGTAGKKKKNLLSDRLIEVIEKNADRIARLWLEDVTT
                     NHSTAGYHHVDRDDLFNRAYGIISQFGRWLGGYYRDQDIRNFYTELGRERHQAGFKLS
                     EVLSAQSLLKKHIWEFALSRGMWQKTLDIYMALELDRRIVVFFDKAAFYTTKGYEGKA
                     GA"
     gene            complement(2822..>3311)
                     /locus_tag="P5573_06000"
     CDS             complement(2822..>3311)
                     /locus_tag="P5573_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27648.1"
                     /translation="LCSYALYFFPEIIPDISRVLKNEGVFVSITHSSGNLGELIDAAK
                     EALRARNMLPGETLPIEGIIRRFSSDNGLQLLSPWFGHIRSVDYPNELIFSPGDLQKI
                     VEYFQFKSPFFLSGTGFSPETVAGLLRRHLERVLHARDSFTISKDDTIFIAEKPLREG
                     GK"
BASE COUNT          675 a          903 c          963 g          770 t
ORIGIN      
        1 ctgaaatggc gcatcccagg tcgtctttgg tcatcgcaat cgtccagccg tacttccggg
       61 cgacggtctg gacctggcag tgagccattt tcaccttcat gtgcttcaac ggggttcgag
      121 tcccttcggg catatcttct ttgtttttca aaaagcggat tgccaccgga aacgtgtcta
      181 cgttgatgta gcccttcagt tgcctcccaa gatcttgcca ttccttcatg gtctcactcc
      241 ttcggtgtct tgaatttacg caaaataccg cccaaaacct tcttgaaggc ttccacttcc
      301 gcaggcgaat aatccttctt gatctcctcg ttgatgcgtt tgatgacggt cagcgccgcg
      361 gccgcttcct tgagacctgc cggcgtcaac aggatcgaaa tcgcccttcg gttttggggg
      421 tcgatctgac ggttgaccag cccggctttc tcgaggcgat ccaccaggcg cgtcattgcc
      481 gaattgtcga ccccgatgat ttgccccatc tccgacatgt agcgccggtc tttcgccttg
      541 agaaggaaca ggatggcggc ctgggcaaga gtcaccttgc cgcctgcctc cgagatgctt
      601 ctgttcgcgt ggtccttcaa ggcctgctga gcgatggaaa gaagataaat caggcgatca
      661 tcgttaccca tagttctcca cacaggggct catgtttgca aataatatag ttgattagtc
      721 aactgtatgt caagcgcttt ctggatgccg ggaaggagcc attgtgcgag tgtttgaagt
      781 gttggctacc gaagaaattc tggaggaata acaaagagaa tctgactgtg ccggcgggct
      841 catggccatg cggttgatcc gggaaagcgg ccggaacgtg tcgcttctct tgcaggattt
      901 caaagcggaa ttcctgaagc gggcggaagg ggatgtgttt ttcacctgcg aggacggccc
      961 ggcgattcgc gccctcgtcg aaaaggccat gacgacggat gaccgtgtca attccccggt
     1021 gcgcgtgacc ctctaaaccg gggtcggggc ccgcggcccg gttcgagctg accctgacat
     1081 tgaaaaagaa gagaaatcag ccctcccatg aatctttccg gctgtaaaaa tcacttaagt
     1141 tttcatgtat ttcgccgata atgtgttcga gaaaggagca ttcggcgaag gaagcgacac
     1201 gcgggtcaat aggatttcag ttcattgaga tgattgggtt tttcataagc ctgataaagg
     1261 caaaccacac cgaaagggtg gggcgcaaag ccgctggcct aagtccgtca cggcggatag
     1321 ggtagcaggg ttgccgggcg agcaatagcc tctgcataac ggtctccatt tgcacgccca
     1381 ggacacaatg tggaggtaac gaaatgcgcg gagcggagga ttattctaca gactggaggt
     1441 acacttctct gggcagcacc gccggccgtc agacggccaa gaaagacata tccattgtcg
     1501 gagtcgtggg agacctcctg ttcatcacag gtctgatttt cctgggattc gagatctttt
     1561 tctgggtccg gcacggggag tggctgggat atccctgcac gctcattctc gatgccgtcc
     1621 cctcggcgtt tctcgcatcc ctcgagaact ccccggtcct gaaaggtatc gtcgtcgggg
     1681 ctctcaagag gctcgatttc tcggtggtcc ttgtcatatc ggggtatatt cttgggcgga
     1741 aattcggaga ccttctctaa ccgtccatag cctgcggaca agacaaaaaa cggggccccg
     1801 aagccccgtt tttctcaggc ccccgctttg ccttcatagc ctttggtggt gtaaaaggcc
     1861 gccttgtcga agaagacgac gattcgcctg tcgagctcga gggccatgta gatgtcgagg
     1921 gtcttctgcc acatgcctct cgagagggcg aattcccaga tgtgtttctt gaggaggctc
     1981 tgggcgctca ggacctcact aagcttgaat cccgcctgat gtctttccct tcccagttcc
     2041 gtgtagaaat tccggatgtc ctgatcccgg tagtagccgc ccagccagcg tccaaactgc
     2101 gatatgatcc cgtaagccct gttgaaaaga tcgtcgcggt cgacgtggtg gtaccccgcc
     2161 gtggaatggt tcgtcgtcac gtcctcgagc cagagacggg cgatgcggtc ggcgtttttt
     2221 tcgatgacct cgatcaaccg gtccgagagg aggtttttct tcttctttcc tgcggtccct
     2281 tccgtcacgg cgagattgaa ggaatcgagg atggcccagg cgtcggcttt gctgcggatg
     2341 aatgcctcga tggccttttt gttcgatgta aaggcaagcc gcgggatgcg atcgaggggg
     2401 aaatatctga cggtgtccgc gtcgcctccg cgggcgagcc tgccgcccgt cgcttccacc
     2461 tcgaaggtca cgaagagaag gtcgccgtaa tagtagttct cggcggagcc gagatccacg
     2521 agacccacga tggatccccg catgcccgtc tcctcctcga gttcccgcag ggccgcgtcc
     2581 tcgatgctct ccccggcctc ggcgaaaccg gtgggcaggc accacatgcc ccggtagggt
     2641 ttcttgccgc gtttgacgag aaggatcttc cggtccgaca gaaggatggt cgagacgacg
     2701 gggagcgggt tttcgtaaaa aaagacgccg caggtctcgc aatagctgcg ggggacgtta
     2761 tcctccctcc gggtcacggt tacgcctccg cagagggagc agaatcgttt gggttgacgt
     2821 ttcatttccc cccctccctc aggggtttct cggcgatgaa gatggtgtca tctttggaaa
     2881 tcgtgaatga atcacgggcg tgaagcaccc gctcgaggtg ccgccttaaa agaccggcga
     2941 ctgtctccgg tgaaaatccc gtcccggaca ggaaaaaggg gctcttgaat tgaaagtatt
     3001 cgacgatttt ctgaaggtcg cccggtgaga agatcagctc gttcgggtaa tccacgctgc
     3061 ggatatgacc gaaccagggg gagaggagtt gaagcccgtt atcggatgag aaccggcgga
     3121 tgatcccctc gatgggcagc gtctctccgg ggagcatgtt ccgggcccgg agcgcttctt
     3181 ttgccgcgtc gatgagctcc cccaaatttc cgctgctgtg cgtgatcgat acgaaaaccc
     3241 cttcgttttt caggacccgc gagatatcgg ggatgatctc ggggaaaaaa taaagggcat
     3301 agctgcaaag a
//
LOCUS       DAOTMX010000185         3290 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_22934_length_3290_cov_4.293663, whole genome shotgun sequence.
ACCESSION   DAOTMX010000185 DAOTMX010000000
VERSION     DAOTMX010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22934_length_3290_cov_4.293663"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..584)
                     /locus_tag="P5573_06005"
     CDS             complement(30..584)
                     /locus_tag="P5573_06005"
                     /inference="COORDINATES: protein motif:HMM:NF022607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2939 domain-containing protein"
                     /protein_id="HRT27649.1"
                     /translation="MDKNEPVRRRRLIVIVSVCLALIIVAVAAFLYVRTTPYYSLHML
                     KKAIVNRDAKGVLRYLDTDSILDNMAKDVFARMDSKEPSRNKFEDHMRSAGKDAARQL
                     LPQLKAQMGEAITDLLLSYNDRQLSANLRKATVFALVVTTHGDTAEVRQRGKDRVSFT
                     MARAPEGHWRITSFNPEELTLLGK"
     gene            complement(596..1042)
                     /locus_tag="P5573_06010"
     CDS             complement(596..1042)
                     /locus_tag="P5573_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27650.1"
                     /translation="MKRYLQNERGGVARWILILIIIAAGFYGYQYVKKTPRYALIQFK
                     KAILFSNAETARTYMDFDSVIRSLPEGVTRGQSDEQLKKRLLYEIDAPHEKSYFKGVK
                     GWSVILVPITVAPDQQTATARPADDTSVTLQRTAGGQWVIVSVEMP"
     gene            complement(1079..2557)
                     /locus_tag="P5573_06015"
     CDS             complement(1079..2557)
                     /locus_tag="P5573_06015"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HRT27651.1"
                     /translation="MSDEQINNQENIEERSATESGHEEDFAQLFEQSTQSVKRLEPGQ
                     RVTTRVAGISGDFVYIDLGGKSEGVIDRKEFLNEEGAVTIQDGDEIEAYFVTVENGAR
                     KFTTLIHSFPSITLQSIKNAFDADIPVTGKVASELKGGYEIRVGKVRCFCPYSQMDLK
                     GSRDAGTYIGETFPFKVLEYKERGRNIILSRRALLEKELEKEKEKLKETLEVGMDIQG
                     KVRSIQRFGVFVNIGGIDGLIPLSELSWIKGAKAADLISIGDEVKTRILEIDWARDRI
                     TLSLKAVLPDPFLSVPEKYPVDSTIQGVVVRLESFGAFVNLEPGIDGLIPISKLGAGR
                     RINHPKEVLEPGQQVEARVVDISTEKRRLTLSMERKIVVEEVDLPAIGTLLETKVERV
                     ISSGIIVRIKEGLTGFIPNVEMGTLKGTNHSKMFPVGSPMQAVVVATDPSRGRVSLSR
                     TKVDEKIELDSYTQYRDKVREEERSDEKLGSLGELLRNKLGL"
     gene            2712..>3290
                     /locus_tag="P5573_06020"
     CDS             2712..>3290
                     /locus_tag="P5573_06020"
                     /inference="COORDINATES: protein motif:HMM:NF013509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase
                     N-terminal domain-containing protein"
                     /protein_id="HRT27652.1"
                     /translation="MGGKEVGVNSFSVVKSCVLACVALFLAVFVATTVPVFAQGKSGP
                     ALSREKTGYALGVSIGRNMKNLGIDIDPDMIARGVRDTVRGQTVMSDQEVKTTLDEYE
                     RDVVKRLGEKNRREGDSFLSENRQRKSIVTLSSGLQYMVLREGKGPVPKPTDLVTIHY
                     KARLIDGTEFESSYWACRNHHPRNRLAAGIARE"
BASE COUNT          659 a          898 c          914 g          819 t
ORIGIN      
        1 gacctgcgac ctgcgacctg cgaccgtttt tacttgccca gcagtgttag ttcttcgggg
       61 ttgaaggagg ttattcgcca gtggccttcg ggagccctgg ccatggtgaa gctcaccctg
      121 tccttgccgc gctggcgtac ctcggcggtg tcgccgtgtg tggtaacgac gagggcgaat
      181 accgtggcct tccgaaggtt tgcggagagt tgccggtcgt tataggagag aagaagatcg
      241 gtgatcgcct ctcccatttg ggccttgagc tggggcagga gctgtctggc ggcgtctttg
      301 ccggcactgc gcatatggtc ctcgaacttg ttgcgggaag gctccttgct gtccattcgt
      361 gcaaaaacat cctttgccat gttgtcgagg atgctgtcgg tgtcgaggta tctgaggacc
      421 cccttcgcgt cacggttcac gatggccttt ttcagcatgt ggaggctgta atagggtgtg
      481 gtgcgaacat aaaggaaggc agcaacagca acgatgatga gtgcgagaca gactgaaaca
      541 atgacgatca gacgtctcct gcgtaccggt tcgttcttat ccatataggg tgcgcctacg
      601 gcatctccac cgagacaatg acccactgtc cgccggcggt tctttgcagg gtcacagacg
      661 tgtcgtcggc gggccgcgcc gttgctgttt gctgatcggg cgccacagtg atggggacga
      721 gtatgaccga ccaccccttc acgcccttaa aatagctttt ttcgtgaggg gcgtcgatct
      781 cgtagaggag acgtttcttc aattgctcgt cggactggcc tcgagtgaca ccctcaggca
      841 atgaccggat aacgctgtcg aaatccatgt atgtccgggc cgtctcggcg ttgctgaaga
      901 ggatggcctt cttgaactgt atcagcgcgt agcggggggt cttcttaacg tactggtagc
      961 cgtagaagcc tgccgcaatg ataatgagga tgaggatcca tcgcgctacg ccgccccgtt
     1021 cgttttggag atacctcttc atgggtctgt cgacctcctc ccttaagggt gcccgctgtt
     1081 acagtccgag cttgttcctc aggagttcac caagactgcc gagcttctcg tcggaccgtt
     1141 cttcttccct taccttgtcc ctgtactggg tgtaactgtc gagttcgatc ttctcatcga
     1201 ccttggtcct cgagaggcta acccggcccc gggacgggtc ggttgcaacg actaccgcct
     1261 gcatgggcga tcccacagga aacatcttgc tgtggttggt gcctttcagg gtgcccatct
     1321 caacgttggg aatgaagccc gtcaatcctt ccttgatcct gacaatgatc ccggaggaaa
     1381 taacgcgctc cacctttgtc tcgaggagcg ttccgatcgc gggaagatct acttcctcga
     1441 ccacgatctt gcgttccatg gagagggtaa gccttctctt ttccgtgctt atatccacga
     1501 ccctggcctc gacctgctgg cccggttcga gcacttcctt gggatgattt atccttctgc
     1561 ccgcccccag cttcgagatc gggatcaacc cgtcgatgcc gggttcaagg ttcacgaagg
     1621 cgccaaagct ctcgagacga accaccaccc cctggatcgt tgaatccacg gggtatttct
     1681 cgggaaccga cagaaaggga tcgggaagga ccgccttaag gctcagcgtg atccggtccc
     1741 tcgcccagtc gatctcaagt atcctcgttt tcacttcatc gccgatgctg ataaggtccg
     1801 ccgccttggc tcccttgatc catgagagct cgctaagcgg tataagaccg tcaatgccgc
     1861 cgatgttcac gaatacgccg aatctctgga tcgaacgcac cttgccctgg atatccatgc
     1921 cgacctcgag ggtctccttg agcttttcct tctccttttc cagctccttt tccagcaggg
     1981 cgcgccgtga gaggataatg ttgcgccccc tttccttata ttccagcacc ttgaaaggaa
     2041 atgtttcgcc aatgtatgta ccggcatccc tgcttccctt gaggtccatc tgagagtaag
     2101 ggcagaagca gcgtaccttg cccacgcgaa tctcgtagcc gcccttgagc tccgacgcca
     2161 cttttcccgt gacgggaata tcggcgtcga aggcgttctt gatgctctgc agcgtgatgc
     2221 tcgggaagct gtggatcagg gtggtgaatt ttctcgctcc attttccacg gtcacaaagt
     2281 aggcctcgat ctcatctccg tcctggatgg tgacggcgcc ttcctcattg aggaattcct
     2341 ttctgtcgat gaccccttca ctcttgccgc caaggtcaat atagacaaaa tcgccggaga
     2401 tgccggcaac ccgggtagtt accctctgtc ccggttcgag tcttttgacc gactgagtgc
     2461 tctgctcgaa aagctgtgca aaatcttctt cgtgtccgct ttctgttgcg ctcctttctt
     2521 caatattttc ctggttgttg atctgttcgt ctgacatcgt ccgatctcct gaagttgtta
     2581 aaaaattttt tgttccagta atgtacaatt tctttagtgt tatgtaaagc tatagttctc
     2641 atggacaatg gatttgacaa ttctaagccc atggaataac atatgaaaca tcaaaaaccc
     2701 attggtcgcg tatgggcgga aaggaggtag gtgtgaattc tttttccgta gtaaaatcct
     2761 gtgttctggc atgtgtcgcg ttgttcctgg cggttttcgt tgcgacgacg gtgcccgtgt
     2821 ttgcgcaagg caagagtgga ccggcgcttt ccagggagaa gaccggatat gctctcggag
     2881 tgagcattgg gcgcaacatg aagaacctgg gtatagatat cgatcccgac atgatagccc
     2941 gcggagtgcg ggacaccgtt cgggggcaga ctgtcatgtc cgatcaggag gtgaagacca
     3001 ccctcgacga gtatgagaga gacgtcgtga aacgactggg cgagaagaac agacgtgaag
     3061 gagatagctt cctcagcgaa aacaggcagc gcaagagcat tgtgaccctc tccagcggtc
     3121 tccaatacat ggtcctgaga gagggcaagg gccctgttcc gaaaccgacc gatctcgtca
     3181 cgatccacta caaggccagg ctgatcgatg gaacagagtt tgagagctcc tactgggcgt
     3241 gtaggaatca tcatccacga aacaggctgg cggcggggat agcgagggag
//
LOCUS       DAOTMX010000186         3271 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_23109_length_3271_cov_3.490361, whole genome shotgun sequence.
ACCESSION   DAOTMX010000186 DAOTMX010000000
VERSION     DAOTMX010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23109_length_3271_cov_3.490361"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /gene="yidD"
                     /locus_tag="P5573_06025"
     CDS             complement(<1..241)
                     /gene="yidD"
                     /locus_tag="P5573_06025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HRT27653.1"
                     /translation="MSGALATVKAATNEQKGRAAPASLLVAAVKFFRAYISAVDGDRC
                     PMYPTCAAYSIQAFEKHGLIAGYLMTVDRLIHENNE"
     gene            complement(355..1257)
                     /gene="bamD"
                     /locus_tag="P5573_06030"
     CDS             complement(355..1257)
                     /gene="bamD"
                     /locus_tag="P5573_06030"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HRT27654.1"
                     /translation="MLAALILLQICAFGAFAADAGDADKLYGFAEKLFGDGEYFRAIS
                     EYKRFIFYYPGDTRLEKASLRIAESYYRAKRWADAVDSVKEFLAKFPESPLTQEALFI
                     KGMSEYNLKRFDESLSTFGNLMSKGSGEYRDRAIYQTAIIYVDREEWDRAKGAFRNIP
                     EGSLLFPFASRFAAGLEKIDILPYKSPAVAGTLAAVLPGSGHLYAERPRDALVAFILN
                     AAFIAAAVELFDHGEYAAGGIVAFFEAGWYSGNIYSAVSSAYKFNKRVKEEFIRNLKD
                     ESPPTPPVGRISLKGNQVMYSFRF"
     gene            complement(1287..1871)
                     /locus_tag="P5573_06035"
     CDS             complement(1287..1871)
                     /locus_tag="P5573_06035"
                     /inference="COORDINATES: protein motif:HMM:NF005322.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HRT27655.1"
                     /translation="MPKDNTTKSILLAGVGGQGILRASDIISLALMTAGYDVKKSEVH
                     GMAQRGGSVTSHVRYGGKVYSPLAARGSVDIMLSFEKLETLRYIDFLKKNASVLINDE
                     AIFPPAVNMGIASYPENIPDIVGKAFKRVKVVQASALAEQAGEARTANTVMIGAVSNY
                     LDVLLEVWEEVIGNSFPERLVEVNIEAFKLGRNA"
     gene            complement(1873..>3271)
                     /locus_tag="P5573_06040"
     CDS             complement(1873..>3271)
                     /locus_tag="P5573_06040"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRT27656.1"
                     /translation="DSREAKDFVKRAFELSALFDTPVFLRTTTRVSHSKSIVLLDDPE
                     KPVKKEVERLDPVKYVMVPHNARMRRVVVEERMQRLRKYVETMPENWMEINDHRFGIV
                     TAGMPYNYAKEVFPDFSYLKLGMVYPLPEKMIKSFAAKVGKIYVVEELDPFLEEQLKA
                     MGIKTAGKDIFPYSGEFDPGVIAEAIKRRSAKKVQLPPVEIPPRPPNLCPGCPHRGLF
                     YVLGKSKTIVTGDIGCYTLSFMKPLEGLQGCICMGASIGMAHGLSKFLGTKGKGKVVG
                     VLGDSTFIHSGIPSLLNTAYNRSNVLTVIADNRTTAMTGMQQHPGTGLTLQGEKTVEL
                     NFETLAKSLGIPNVRVVDPYDLKGTRAAVREEIGKPGPSLIISRAPCVLLNRGAARKR
                     PFKVVEEKCAGCKLCLGLGCPPISWKKYPAATERMLEGKPKKVEGAAVIDTSLCTGCS
                     LCEQVCNYGAIVEEE"
BASE COUNT          656 a          922 c          935 g          758 t
ORIGIN      
        1 tttcgttgtt ctcgtggatc agtcggtcga ctgtcatgag ataaccggcg ataagcccgt
       61 gtttctcaaa ggcctggata ctgtaagccg cacaggtcgg gtacatggga caacggtctc
      121 cgtcgacggc agaaatatag gcgcggaaga acttcactgc agccacgagc aatgatgctg
      181 gtgccgccct acccttctgc tcattggtcg ctgcctttac cgtcgcgagt gcacctgaca
      241 tttctttgtc gaagtcccag ggttcaaagt cttccgaccc ggcaagcgaa ggcaccgaga
      301 tgattacaaa aaatacaatt aagactaatg ctgcgatata tcgcatagta aagactaaaa
      361 tctaaagcta tacatcacct gattgccttt tagagagatc cttccgaccg gcggcgtcgg
      421 ggggctttcg tccttcaggt tgcggatgaa ttcttccttc acgcgtttgt tgaacttata
      481 ggcgctgctc acggcactgt atatgttgcc gctgtaccat cccgcctcga agaaggcgac
      541 tattccgcca gccgcatact ctccgtggtc gaagagctct accgcggcgg cgatgaaggc
      601 ggcgttcaag atgaaggcca ccagggcgtc tcgcggcctc tcagcgtaaa gatgtcctga
      661 accgggtagg acggctgcca gtgtcccggc gacggctggg gatttatagg gcaggatatc
      721 gattttctcc aggcctgcgg cgaaccgact cgcaaaaggg aacagaaggc tgccctccgg
      781 gatattcctg aaggcccctt tcgccctgtc ccattcctcg cggtcgacat agatgatggc
      841 ggtctgatag atggcccggt ctctgtactc cccgctgcct tttgacatga gattgccgaa
      901 agtcgagagg gattcgtcga agcgcttcaa gttgtactca gacataccct tgatgaagag
      961 ggcctcctgt gtcagggggc tttcagggaa cttggcgaga aactccttga ccgaatcgac
     1021 cgcgtcagcc caccttttcg cccggtagta gctctcagct atcctcaacg aggccttctc
     1081 gaggcgtgta tctcccgggt agtaaaagat aaaccgcttg tattcggaaa tcgcccggaa
     1141 gtattcgccg tcgccaaaga gcttctccgc aaagccatag agcttgtcgg catcgcccgc
     1201 gtccgccgca aaggcaccga atgcgcagat ctgcagcaga atgagtgctg cgagcaccgg
     1261 cgggaataat aatttacaga ggagtttcaa gcattacgcc cgagtttgaa tgcctcgatg
     1321 ttgacctcga ccagtcgttc agggaaggaa tttccgatga cttcttccca tacttcaagt
     1381 aatacatcaa gataattgga aacggccccg atcatgacag tgttcgcagt ccgcgcctct
     1441 cctgcctgct cggccagggc ggaggcctgg acgactttta cccttttgaa tgcctttccc
     1501 actatatccg ggatattctc ggggtaagag gcaatgccca tgttgactgc aggaggaaag
     1561 atggcctcgt cgttgatgag gacagaggcg ttctttttga ggaagtcgat gtagcggagg
     1621 gtctcgagtt tttcgaaaga aagcatgatg tcgacggagc ctctggcggc gagcggcgag
     1681 tagaccttcc cgccgtacct cacgtggctc gtcacgctgc cgccgcgctg ggccataccg
     1741 tggacctcgc tcttcttgac gtcgtacccg gcggtcatca gtgccaggga tatgatgtcg
     1801 ctcgcccgga ggatcccctg ccctcctacg ccggcaagca gtatgctctt tgtggtattg
     1861 tctttcggca tgtcactcct cttcgacgat ggcgccgtag ttgcaaacct gctcacagag
     1921 ggaacacccc gtgcacaggg aagtgtcgat cacggctgcg ccctcgactt tcttcggctt
     1981 tccttcgagc atgcgttcag tagccgcggg gtactttttc caggatatag gcgggcaacc
     2041 gagcccgaga cacagtttgc acccggcgca tttctcctcg acaaccttga acggcctttt
     2101 tctcgcggca ccgcggttca agagcacgca gggggcccgg gagatgatga gcgaaggacc
     2161 tggtttgcct atctcctcgc gtactgccgc ccgcgtcccc ttcaagtcgt aaggatcgac
     2221 caccctgacg ttagggatgc ctaaggactt ggcaagggtc tcaaagttca attccaccgt
     2281 cttttccccc tgaagggtca ggcccgtccc cgggtgctgc tgcatcccgg tcatcgcggt
     2341 cgtcctgttg tcggcgatca cggtgaggac gttgctcctg ttgtaggcgg tgttcagaag
     2401 cgatggtatg cccgaatgga tgaaggtgga atcgccgagg accccgacca ccttcccttt
     2461 ccccttcgtg ccgaggaact tgctgaggcc gtgggccatc ccgatgctcg cgcccatgca
     2521 gatacagccc tgaaggccct cgaggggttt catgaaagag agggtgtaac agccgatgtc
     2581 gccagtgact atggtctttg acttgccgag gacgtagaag aggccccggt gcggacagcc
     2641 gggacagagg ttcgggggcc gcggggggat ctcgacgggc ggaagctgta ctttcttcgc
     2701 ggagcgcctc ttgatggctt ctgcgatgac gccggggtcg aattcgccac tatagggaaa
     2761 gatgtctttc cccgccgttt tgatgcccat cgccttcagt tgctcttcca ggaaagggtc
     2821 taactcttcg actacgtaga tttttccgac cttcgcggca aaagacttga tcatcttttc
     2881 ggggagcggg tagaccatac cgagtttcag gtaggagaaa tcgggaaaga cctcctttgc
     2941 gtagttgtaa ggcatccccg ccgtcacgat gccgaaacgg tggtcgttga tttccatcca
     3001 gttttcgggc atcgtctcga catatttccg cagcctctgc atccgctctt cgaccacgac
     3061 acgcctcatc ctggcgttat gggggaccat cacgtatttt accgggtcga ggcgctcaac
     3121 ttcctttttt acgggcttct cggggtcatc gagaaggacg atgcttttcg aatgggagac
     3181 gcgggttgtg gtcctcagga agacgggggt gtcgaagagg gcgctcagct cgaaggccct
     3241 cttgacgaag tccttggcct cccgactgtc g
//
LOCUS       DAOTMX010000187         3260 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_23193_length_3260_cov_4.296724, whole genome shotgun sequence.
ACCESSION   DAOTMX010000187 DAOTMX010000000
VERSION     DAOTMX010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23193_length_3260_cov_4.296724"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..833)
                     /locus_tag="P5573_06045"
     CDS             complement(120..833)
                     /locus_tag="P5573_06045"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRT27657.1"
                     /translation="MINIMIDGRKVKAAEHSTVLKNLQDQKIEVPTLCFHKDLLPFGA
                     CRLCTVEVKTDGKWQLASSCNTPVVPDMEIRTDSKKVKESRKLAAELLYYRYPTTKAV
                     RDIAVGLGIEVEPVKVEGNDCILCGLCIRACHEVVGVSALTFLDRGLDRDVDEPKVDY
                     LPTACIACGSCAFICPTGFVKMEETRGGKRRKIWGKEFKMAACKVCGRYFAPLDQLKW
                     ISKTTKTPLKKLMTCPSCK"
     gene            complement(922..2838)
                     /locus_tag="P5573_06050"
     CDS             complement(922..2838)
                     /locus_tag="P5573_06050"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRT27658.1"
                     /translation="MNTVSFSSWNGKIVDNRNGQVPASKLAEVNFPEKLDGQEPAAVM
                     GWDGIVVRDAQADLLSLTLAYLKEARKLSCGECSVCMIGIDKVIGIMKAMMEGQGSAE
                     NVEEIEEIARGVAANSKCNYGRTTALTPVLDAVRYFRDDFLALANGEKKVSEKAYSSA
                     VTAPCMEACPAKLDIPGYIELIRNNRFGDSLNLIRKRCILPGVLGRACTHPCESACVR
                     NDIDETLAIRLLKRAAADYDLGRDGSALGKPAAEREERVAIVGAGPAGLAAAYHLRLM
                     GYQVTVFEALPVAGGMAMVGIPAYRLPRNVLNHEVELIEKMGVDIRLNSKVTQLDWDG
                     LQQQGYKALFLAVGAHVGTRVGCSGEDAGSEDFVQGAEFLRKIALGGKVQPRNKVVII
                     GGGNVALDCARSCLRLGFKDVEILYRRSRKEMPARPEEIREAEEEGVKFNFLKAPVSV
                     IMKEGKVVEVECIKMRLGEPDESGRRRPVEIPGSEFVMKTDMVIAATGQVPDLKLLEK
                     NAINVTNWGTIQVDPVGYGTNIPGIFAGGDCVTGPATLIEALDAGNKVARSIDAFIQG
                     RDFRKEFEFNDIKTGEQRDIGFVPAMPAKKPVFLEVSKRLNNFDEVEGGFSAAEAIDE
                     ARRCLRCYRLLVWE"
     gene            complement(2885..>3260)
                     /locus_tag="P5573_06055"
     CDS             complement(2885..>3260)
                     /locus_tag="P5573_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416000.1"
                     /note="catalyzes the formation of acetyl-CoA from pyruvate
                     and coenzyme A; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HRT27659.1"
                     /translation="YVATACPSYPIDLVNKVKKARDLKGPAYLHVFSMCPTGWRSSSE
                     TVIKLGRLAVETGVFPLYEVENGKYRITQEIPKKLRPVKDYLELQGRFRHLTPDDMAL
                     VQARVDREWRKLMNKVECLRPW"
BASE COUNT          642 a         1014 c          851 g          753 t
ORIGIN      
        1 ctcgaaccac ggacccgctg gttaacagcc agctgctcta ccgactgagc taccgaggag
       61 catttccgga ccgccctttt ctcgtcaaga ccgagggcgg ttttagtctt ctggtgccgc
      121 tatttacagc tagggcaagt catcagtttc ttcaaaggcg tcttggtcgt cttactgatc
      181 cacttcagtt ggtcaagggg tgcgaagtac cgaccacaaa ccttgcatgc cgccatcttg
      241 aattccttac cccagatttt ccgcctctta cccccacggg tttcctccat cttcacgaaa
      301 cccgtcgggc agataaaggc acaggaaccg caggcgatgc aggctgtagg caagtaatcg
      361 accttcggct catccacgtc acggtcgagc ccacggtcca ggaacgtgag ggcgctcacg
      421 ccgacgactt catggcaagc cctgatgcag agtccgcaga ggatacagtc attgccttca
      481 actttcacag gctcgacctc gattccaagg ccgaccgcga tatcgcgaac ggctttcgtc
      541 gtgggatagc ggtagtagag aagttccgcc gccagtttcc ggctctcttt cacctttttc
      601 gagtctgtcc tgatttccat gtcggggacg acgggggtgt tgcaggagga agcaagctgc
      661 cacttcccgt cggtctttac ctccacagtg caaagacggc aggccccgaa ggggagaagg
      721 tccttgtgaa aacacagggt cggaacttct atcttctgat cctggagatt tttgaggacc
      781 gtcgaatgtt cagcggcctt aaccttgcgt ccgtctatca tgatatttat catttacaga
      841 accccttgct gatatagctc aaagtttgaa gtccagggtg taaagaagac aatcccggcc
      901 ttttgaactt tgaatcgttc cttattccca cacaagcagc cggtagcagc gaaggcagcg
      961 cctggcctcg tctatcgcct ctgccgcgct gaatccgccc tccacttcgt cgaaattatt
     1021 gaggcgtttg ctcacttcga gaaacactgg tttctttgcc ggcatcgcgg gaacgaaccc
     1081 gatatcccgc tgttcgcccg tcttgatgtc gttgaattcg aattccttcc gaaaatctct
     1141 gccctggatg aaggcgtcga tgctccgggc aaccttgttg cccgcgtcca gcgcctcgat
     1201 caacgtagcc ggtccggtca cacagtcgcc gccggcaaag attccgggga tgttggtacc
     1261 ataacccacg gggtccacct ggatggtccc ccaattcgtc acgttgatag cgttcttctc
     1321 caggagtttg aggtccggca cttgaccagt agccgcgatg accatgtcgg tcttcatgac
     1381 gaactccgac ccgggtatct cgaccggcct tctacggccg ctctcgtcag gctcgccgag
     1441 tctcatcttg atgcactcga cctccacgac cttcccttct ttcatgataa cactgaccgg
     1501 ggccttgagg aagttgaact taactccctc ctcctcggcc tcccgaatct cttccggacg
     1561 cgccggcatt tccttccgcg aacgacggta gagaatctcc acgtccttga accccagcct
     1621 cagacagcta cgggcacaat caagggccac gtttccgccg ccgatgataa cgaccttgtt
     1681 ccggggctgc accttgccgc cgagggcgat tttcctcaga aattcggcgc cctggacgaa
     1741 atcttccgac ccggcgtctt ctcccgagca cccgacccgg gttcctacat gggcaccgac
     1801 ggcgaggaac agggccttgt agccctgctg ctgcaaaccg tcccagtcga gttgtgtcac
     1861 cttgctgttc agccggatgt ccacacccat cttctcgatc aactcgacct cgtgattgag
     1921 gacgtttctg ggcaaccggt aagcaggtat cccgaccatc gccattccac cggccacggg
     1981 cagggcctcg aagaccgtga cctgataccc cataaggcgc agatggtaag ccgcggccaa
     2041 tcctgccgga ccggctccga caatagctac tctttcctcc ctttccgccg ccggtttgcc
     2101 gagggcgctc ccgtcccggc caaggtcgta gtccgccgcc gcgcgcttca ggagtcggat
     2161 cgcgagggtc tcgtcgatgt cgttgcggac gcaggcgctc tcacagggat gggtgcaggc
     2221 acgtccgagc accccgggga ggatacaccg cttgcggata aggttgagcg aatcgccgaa
     2281 ccggttattc cggataagct cgatgtagcc cgggatgtcg agcttcgcag gacaggcttc
     2341 catgcatgga gcagtcacgg cggatgagta cgccttttcc gagaccttct tttcaccatt
     2401 tgccagggcg aggaaatcat ccctaaaata gcggacggcg tcgagaacgg gtgtgagcgc
     2461 cgtggtgcgg ccgtagttac atttgctgtt ggcggccacg cctctggcga tttcctctat
     2521 ctcctccaca ttctccgcag acccctgccc ttccatcatg gccttcatga tgccgatgac
     2581 cttgtcgata ccgatcatgc acactgagca ctcaccgcag gagagttttc ttgcttcctt
     2641 gagataagcg agggtcaggg acaagaggtc cgcctgggca tcacggacga cgatgccgtc
     2701 ccagcccatg acagccgccg gttcctgccc gtcgagtttt tcaggaaagt tcacttccgc
     2761 gagcttcgac gcagggacct ggccattcct gttatcgacg atcttgccgt tccaactgct
     2821 aaacgaaact gtattcattc ttatgtaccc taccgttttt attttgacgt cggtgataaa
     2881 agcgttacca gggtctaaga cattcgacct tattcatcaa tttccgccac tctctgtcca
     2941 ccctggcctg aaccagtgcc atgtcatcag gcgtaagatg cctgaaccgc ccctggagtt
     3001 ccagataatc ttttacgggg cgaagttttt tcggtatctc ttgggtgatc cggtatttgc
     3061 cgttctccac ttcatagaga ggaaagacgc ccgtctccac ggcaaggcgc cccagcttga
     3121 tcacggtctc cgaggagctc cgccagcccg tggggcacat cgaaaagacg tggaggtaag
     3181 cggggccttt caggtccctc gccttcttca ccttgttcac caggtcgatg ggataactcg
     3241 gacaggccgt cgcgacatag
//
LOCUS       DAOTMX010000188         3240 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_23371_length_3240_cov_4.406907, whole genome shotgun sequence.
ACCESSION   DAOTMX010000188 DAOTMX010000000
VERSION     DAOTMX010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23371_length_3240_cov_4.406907"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="P5573_06060"
     CDS             complement(<1..749)
                     /locus_tag="P5573_06060"
                     /inference="COORDINATES: protein motif:HMM:NF015949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HRT27660.1"
                     /translation="MKKGEILRRIYERVCGDADTPVQDIRIGICYVGVRVFEDRMGLA
                     ATFLSGVTGDCLRFPYAGRLANSSAADLLRFLLEAKNPVESALGLATANAILQTGIRG
                     GKEEDALQLMNLTPADRVAMVGFFPPLVPKIKASGALLDVIERNPARMPLLDEEERKA
                     ILRSCTVAVITATALLNGTMEETLKELGSPRHVAVLGPSTPLCLEVFAGTPVTHLGGA
                     LVSDTGKTLQVISEGGGTPQMRPYLRFVNLL"
     gene            928..1791
                     /locus_tag="P5573_06065"
     CDS             928..1791
                     /locus_tag="P5573_06065"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HRT27661.1"
                     /translation="MKKALLVVLLLVLTVSHVYAAQSAITEAEGYACQGEDKSRRQTE
                     QAATADAKRKAIEQVSAYVTSETEVKDFTLQKDLAQAYSRATVKVIQELEKGWYRDAS
                     LGDCYRIKVKAEVIPDEQEMMAIAKGKTADDPSAPLCVRVWTDKQAYGAGERVKVYLK
                     GNKPFYATILYRDAGGKLVQLLPNPHRKDNYFNGGVVYEVPAGNDRFELEVAPPFGEE
                     QVIVYAGTTPPGDVSVVERGGVYEVKTRASEVGERTRGVKLTQKLSAAGAVAAEFFEG
                     KATLTTEGSKR"
     gene            1788..>3240
                     /locus_tag="P5573_06070"
     CDS             1788..>3240
                     /locus_tag="P5573_06070"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27662.1"
                     /translation="MKRRFCEVLILSIIILAVGACAGKLPTEPTLRIETGMHSAPITR
                     IGVDVGNRYLVTGSNDKTVRVWELATGRLLQTLRPPIGGVDEGKIYAVALSPDGRTVA
                     AAGQTGYEWGWNVSIYFFDRASGRMIKKMSGLPRVISNLTYSRNGRYIVAGLVSKKGI
                     RVYRTTDYRLVLKDRDYGDSCYGADFDKTGRLVTTSWDGYVRLYANDFKLITKEEAPD
                     GKRPHGVSFSPDGAKIAVGFTDSTKVDVLSGEDLSYLYSPDTGDITNGNMSTVSWSQD
                     GSYLYAGGRAQKFVNEELKCITRKWRDEGSGGYVDLPAAENTIMQIIPLRSGGIVFGS
                     ADPAFGVFDASDRLAVYKGPEIADLRGNREGFLVSDDGATVQFGYERFGKSSARFSVS
                     ERLLELNPGPSDSQNLNPPVTSTADLNIADWKNTYQPKLSGRPLKLEQYEISRSLTVS
                     PGGKTFLLGTEWYLRLFDGEGTEIWKVSIPDSAG"
BASE COUNT          685 a          913 c          997 g          645 t
ORIGIN      
        1 ctcaggaggt tcacgaaccg caggtaaggg cgcatctggg gagtgccgcc gccttcggaa
       61 atgacctgca aggtttttcc tgtgtcggag acgagcgccc cgccgaggtg ggtcaccggc
      121 gttccggcga acacctcgag gcaaagaggt gtggagggac ccaggaccgc cacatgccgg
      181 gggcttccca gttccttcag tgtttcctcc atcgtgccgt tcaggagcgc cgtggccgtg
      241 atgacggcga ccgtacaact cctcaggatc gccttccttt cttcttcgtc gaggagcggc
      301 atgcgcgccg ggtttcgctc gatgacgtcg agcaaggccc ccgaggcctt tatcttcggg
      361 accaggggcg ggaaaaaccc caccatggcc acccggtcag cgggcgtcag gttcatgagc
      421 tgcagggcgt cctcttcttt tcccccccgg atgcccgtct ggaggatggc gtttgcggtt
      481 gcaagtccga gggcgctctc taccgggttt ttcgcctcca ggagaaatct caggaggtcg
      541 gctgcggagg aattcgccag ccttccggcg taggggaagc gcaggcagtc gccggtgacc
      601 ccgcttaaga acgtcgccgc caggcccatc cggtcctcga acacccgaac gccgacatag
      661 cagattccga tgcgtatatc ctgaaccggt gtatccgcgt cgccgcaaac tctctcgtat
      721 atgcgcctca gtatctcgcc ctttttcatc gtcggcctcc cccgagggtc ttttgcgggg
      781 gcatcttagc ccttttcccc ggaccttgca accgcatccg tcggttctgc atccgtcggt
      841 tctcacgcgg aattccttga ctccctgcgg gatcgtctgt agggtaaaac catcgactca
      901 ccggttcttc ggggaaggag gcttgagatg aagaaggcgc ttctcgtggt tttgctgctt
      961 gtcctcacgg tttcccatgt ctatgccgcc cagtccgcga tcacggaggc ggagggttac
     1021 gcctgccagg gggaggacaa gtcgaggagg cagacggagc aggccgcgac ggccgacgcg
     1081 aaacgcaagg ccatcgagca ggtctccgcg tatgtcacga gtgagacgga ggtaaaggat
     1141 ttcaccctcc agaaggacct ggcccaggcc tactcgcggg cgaccgtcaa ggtcatccag
     1201 gagttggaga aggggtggta ccgggatgcc tccctcggcg attgctaccg gataaaggtc
     1261 aaggccgagg tgatccccga cgaacaggag atgatggcga ttgcgaaggg caagacggcg
     1321 gacgacccat cggcgccgct ttgcgtccgg gtctggacgg acaaacaggc ctacggggcg
     1381 ggcgagcggg tcaaggtcta cctcaagggc aacaagccct tctatgccac gatcctttat
     1441 agggatgccg gagggaagct cgtacaactc ctccccaacc cccacaggaa ggacaactac
     1501 ttcaacggcg gagtcgtcta tgaggtcccc gcgggcaacg accggttcga gttggaggtg
     1561 gccccgccct tcggcgagga gcaggtcatc gtctatgcgg gcaccacccc gccgggagac
     1621 gtaagtgtag tggagcgggg cggggtgtac gaggtcaaga cccgggcctc cgaggtcggt
     1681 gaacggacac ggggcgtcaa gctcacccaa aaactcagcg ctgccggtgc ggtagccgcg
     1741 gagttcttcg aggggaaggc gaccctgacc acggaaggca gtaagcgatg aagagacgat
     1801 tctgcgaagt cttgattctt tctatcatca ttttagcggt gggggcgtgt gcagggaagc
     1861 tgccgacaga gcctacctta aggatcgaga cgggcatgca ttccgctccc ataacgagga
     1921 tcggcgtgga tgtcgggaac cgctatcttg tcacgggttc taacgacaag acagtccggg
     1981 tctgggaact agcgacgggg aggctcttgc agaccctgag gccccccatc ggcggtgtag
     2041 atgagggaaa gatctatgcc gtagcccttt ctcccgacgg aaggacggtc gctgccgccg
     2101 gtcagacggg gtatgaatgg ggttggaatg tctccatcta ctttttcgac cgcgcgagcg
     2161 gacggatgat taaaaagatg agcggactgc ctcgggttat ctcaaacctc acctattccc
     2221 ggaacggaag gtacatcgtc gctggtcttg tgagcaaaaa gggcatccgg gtctaccgga
     2281 ccaccgacta tagacttgtt ttgaaagaca gagattatgg agactcatgt tacggcgccg
     2341 acttcgataa aacagggcgg ctcgtgacga catcgtggga tggttatgtc aggctttatg
     2401 ccaatgattt caagctaatc acgaaagagg aggcacccga cggaaagaga cctcatgggg
     2461 tcagcttttc ccccgacggc gcgaagatcg ccgtcggttt tactgattct acaaaggtag
     2521 acgtcctctc cggcgaagac ctctcctacc tctattcccc cgacaccggc gacatcacaa
     2581 atggcaatat gtcaacggtc tcctggtctc aggacggaag ttacctttat gccggaggac
     2641 gggcgcaaaa gtttgtgaat gaagaattga aatgcatcac ccgcaaatgg cgcgatgaag
     2701 gtagtggggg ttacgtggac ctaccggcgg cggaaaacac gatcatgcag attattcccc
     2761 tgcgatccgg cggcatcgtc tttggctccg ccgaccctgc attcggcgtc ttcgatgcct
     2821 cagaccgatt ggcggtgtac aaaggaccag agattgcaga tttaaggggt aatcgggaag
     2881 gattcctcgt ttccgatgac ggcgctaccg tccaatttgg ctacgaacga ttcggcaaat
     2941 catcggcgag attctccgta tccgaaaggc tcctggagct gaatccaggc ccttccgatt
     3001 cgcagaactt gaaccccccg gtgacttcaa cagcagactt gaatatcgcc gactggaaaa
     3061 atacttatca gccgaagtta agcggcagac cattaaaact ggaacaatac gagatatccc
     3121 gcagtctcac cgtttccccg gggggaaaga cctttctctt ggggacagaa tggtatctcc
     3181 gcctgttcga cggcgaaggg acggagatat ggaaggtttc tattccggat agtgcagggg
//
LOCUS       DAOTMX010000189         3231 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_23443_length_3231_cov_3.975126, whole genome shotgun sequence.
ACCESSION   DAOTMX010000189 DAOTMX010000000
VERSION     DAOTMX010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23443_length_3231_cov_3.975126"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="P5573_06075"
     CDS             complement(<1..263)
                     /locus_tag="P5573_06075"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27663.1"
                     /translation="MMKKKSEDRKDKDRKSVEAGHSGPQEGISPAEQPAGEDLAAKLR
                     EKEKEAAENYERYMRAVADLENYKKYAAKDRADILKYANERII"
     gene            complement(274..885)
                     /locus_tag="P5573_06080"
     CDS             complement(274..885)
                     /locus_tag="P5573_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HRT27664.1"
                     /translation="MEDVEDIVVRLFRESNQVKEAFLNDNLSKLVKVVDAVTAALKGG
                     KKVLLFGNGGSAADAQHIAAEFVNRFQIERPPLPAIALTTDTSIITSIANDYDFSEVF
                     DKQIRAIGQPGDIAWGFSTSGSSPNVVKALETAKKMGMVTVGLTGKDGGRIAAIVDVA
                     LNVPSGSVPRIQEAHITVGHVICEMVEFRLFKRPDAKQSGRNG"
     gene            complement(1019..3067)
                     /gene="nrdD"
                     /locus_tag="P5573_06085"
     CDS             complement(1019..3067)
                     /gene="nrdD"
                     /locus_tag="P5573_06085"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HRT27665.1"
                     /translation="MTDKEATEISLFVRTSNEEVVEWDRRRIVDALVLEADLDEETAG
                     EISREVERQVFASGIVTLTAPLIRELVNAKLIERGFEEARRLHTRLGFPLYDIGRLIV
                     SRNKENANVPHRPEGTNLTLAEGIKREYALHSVFSRDVAEAHLSGDIHLHKLGHVDRP
                     YCSSVSLEYVKKFGLSLPGSSSAARPARHADVLLAHMVRFSAVLQGNFAASLEWDAVN
                     FSFAPYLAGWGDAEVGQLAQRLVYEFSQLAFARGGQIMFSDINLYWDVPPHLQGSPVI
                     GPGGEPTGTGFADYRADARRFLRALMEVFREGDAAGRPFFFPRPIFHLTGAVFDDPEG
                     RAFLLDVCETAAEKGNVSFVFDRADGLRVSSRYRIPAWTGGVVAMPWETRWASIGTVT
                     LNLPRLGYRAERNDERLFSLLGEEVELAAKAQAQKKDFIEKLLGYGDEGPLSFLAMEK
                     GGHPFLRMDQAVYPVGVVGLNELVLIHRGSQLHESPEALRFGLKVVSHLKKLVDGLNR
                     KLRMRLILEQTPAESTAYRFARLDLRSFAPRSGRFICGDISRGGVYYTNSTQFSVKAD
                     VSPMDRVEGEGRFHPLIAGTVNTSIWLGEGRPQAEVLAGFIESTFRNTANDQITFSPD
                     FTSCSRCMRTFRGHWDDCPLCGSQAVEKITRIPGYYARVSDLNRGKLAELKDTRRLTF
                     "
BASE COUNT          632 a          985 c          887 g          727 t
ORIGIN      
        1 tttatgattc tttcattggc gtacttgagt atatccgccc ggtcctttgc cgcgtacttc
       61 ttgtaattct caagatccgc aaccgccctc atgtatctct cgtaattttc cgccgcttcc
      121 ttctcctttt ccctgagctt tgctgcgagg tcttcacccg ccggctgctc ggccggggaa
      181 ataccctcct gcgggccgct gtgacccgct tcgacgcttt tcctatcctt atcttttctg
      241 tcttctgatt tctttttcat catcgattca aaatcacccg tttcgtcctg attgtttcgc
      301 gtccggcctc ttaaacaacc taaattcgac catctcacag atgacatggc cgacggttat
      361 gtgcgcctcc tggatccggg gtacactacc cgacgggaca ttcaacgcaa cgtccactat
      421 tgctgcgatc cgacccccgt ctttcccggt caatcctacg gtaaccatcc ccattttctt
      481 tgccgtttcc agggccttaa cgacgttcgg ggaagacccg ctcgtgctga acccccaggc
      541 gatgtctccc ggctgtccga tcgcccgaat ctgcttgtcg aacacctcgg aaaaatcata
      601 gtcatttgcg atgctagtga tgatcgaggt gtcggttgtc agagctatcg ccggcaacgg
      661 cggcctctcg atctggaagc gattgacgaa ctcggcggct atgtgctggg catccgcggc
      721 gcttcccccg ttcccgaaga gaagaacctt cttgcctcct ttcagcgctg ccgtcacggc
      781 atcgaccacc ttaacgagtt tactgagatt atcgttaagg aaggcctctt tgacctgatt
      841 gctttccctg aacagccgga cgactatatc ttcgacatct tccatctatc gccccaatat
      901 cactgattta ttcctaacgg ctgccgccct ttcaactgtt cactaattta ttaatcgctc
      961 cctgcggtgt caagggtgac agaaaacttt taggaaagag aaaatctatg gttatgcttt
     1021 agaacgtgag tcgtcgcgtg tccttcaact cggcgagctt gccacggttg aggtccgaca
     1081 cccgggcgta atacccgggg attctcgtta tcttttccac ggcttgcgac ccacagaggg
     1141 gacagtcgtc ccagtggccc ctgaacgtcc tcatacaccg ggagcaactc gtgaaatcgg
     1201 gcgaaaaggt gatttgatcg ttcgcagtgt ttcggaaggt cgattcgatg aagcccgcga
     1261 ggacctcggc ctgagggcgc ccttccccca gccagataga cgtattgacc gtccccgcga
     1321 tcaggggatg aaaacgccct tccccttcaa ccctgtccat cggcgataca tcggccttga
     1381 cactgaactg ggtcgagttg gtgtagtaga ccccaccccg ggagatgtcg ccgcagatga
     1441 accgtcccga tcggggggca aaggaccgca ggtcgagtct cgcaaagcgg taggccgtgc
     1501 tttccgccgg cgtctgttcc aggatgagac gcatcctgag cttcctgtta aggccgtcga
     1561 ccagcttctt gagatggctg acgaccttca ggccgaaacg cagggcttcc ggcgattcgt
     1621 ggagctgact ccccctgtgt atgaggacaa gctcattcag gccgacgact cccaccgggt
     1681 agacggcctg atccatccgc agaaaggggt ggccgccctt ctccatcgca aggaaggaaa
     1741 gggggccctc gtccccataa cccagcaact tctcgataaa atctttcttc tgtgcttgcg
     1801 ctttcgcggc aagttccacc tcttcgccaa ggagagaaaa gaggcgttcg tcattccttt
     1861 cggcccggta gccgagccgc ggcaagttca gcgtcacggt tccgatggac gcccaacggg
     1921 tctcccaggg catcgcaacg accccgcctg tccaggccgg gatgcggtaa cgggaggaca
     1981 cccgtaggcc gtccgcccgg tcgaaaacga agctcacatt ccccttttcg gcggcggtct
     2041 cgcaaacatc caaaaggaag gccctccctt ccgggtcatc aaaaaccgcc cctgtcaggt
     2101 ggaagatcgg ccgggggaaa aagaagggcc ttcccgcggc gtccccctcc ctgaagacct
     2161 ccatcagggc gcggaggaat ctcctggcgt ccgcccgata gtcggcgaag cctgtccccg
     2221 tcggttcccc gcccggcccg atgacggggg aaccttggag atggggcggc acatcccagt
     2281 agaggttgat gtccgagaac atgatctgtc cacccctggc aaaggcgagt tgggagaact
     2341 catagaccag ccgttgggca agctggccga cttccgcatc accccacccg gcgaggtagg
     2401 gggcgaagga gaaattgacg gcgtcccact cgagcgaagc ggcgaagttt ccctgaagca
     2461 ccgcgctgaa ccggaccatg tgggcgagga ggacgtccgc gtgcctggcc ggcctggccg
     2521 ccgaggagga cccgggaagg ctgaggccga atttcttaac atactcgagg gacacactag
     2581 agcagtaagg ccggtctacg tggccgagtt tgtgcaaatg gatgtcaccg gagagatggg
     2641 cctcggctac gtcccgggaa aagacactgt ggagggcgta ctccctcttg atcccctcgg
     2701 caagcgtgag gttcgtccct tccggccggt gagggacatt ggcgttctcc ttgttcctgc
     2761 tgacgatcag cctgccgatg tcgtaaaggg gaaaaccaag ccgggtgtgg agtctccttg
     2821 cctcttcgaa gccccgctcg atgagctttg cattgacgag ttcccggatg agtggtgccg
     2881 tcagcgtgac tattcccgaa gcgaagacct gcctttccac ctcgcggctt atttccccgg
     2941 ccgtctcttc gtcgaggtca gcctccagga ccagggcatc gactatgcgg cgccggtccc
     3001 attcgacgac ctcttcattc gatgtcctga cgaagagact gatttccgtc gcttccttgt
     3061 cggtcataga taaaagctca aagtgctcgc tccaaagttc acggggaggg gatatcgatg
     3121 ttcctggggt tcgtcagttc ttagccatga gccggcggac ggccttatcc aggccgtcaa
     3181 gagtgagctc gtacatagga aagatttggc ggattatatt tatggtggcc g
//
LOCUS       DAOTMX010000190         3225 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_23508_length_3225_cov_3.230599, whole genome shotgun sequence.
ACCESSION   DAOTMX010000190 DAOTMX010000000
VERSION     DAOTMX010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23508_length_3225_cov_3.230599"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="P5573_06090"
     CDS             complement(<1..515)
                     /locus_tag="P5573_06090"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRT27666.1"
                     /translation="MKEPVIVFDGVTKTYPYYGYITAGLKMFLFNLPEAINHTKKSFT
                     ALKEISFEVYGGECFGIIGNNGAGKSTLLGLAAGALKPDMGTIEIKGRVSPLLELGAG
                     FHPELTGRENIVLNGVLLGMTRKRVVQKMDEIIGFSELEEFIDQPLRTYSSGMVTRLG
                     FSVVAHLDPEIL"
     gene            complement(512..1288)
                     /locus_tag="P5573_06095"
     CDS             complement(512..1288)
                     /locus_tag="P5573_06095"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRT27667.1"
                     /translation="MESLVQNFAYYRDLILVLTAKEIKVKYKNSILGYVWSVLNPLCL
                     AVVFYFAFKFVMKVPVENFTLFLISGLFPWQWFSTSVNASITALFGNASLVKKVSFPR
                     YFIPLTTVLNDTFHFVVSLPIVFAFVVFYKVHLTAAWVYGIPLLMVSQFFITYGCALA
                     VSALNLFFRDLERLVMIGLTVLFYMTPIIYDISLVPERYRQYFLLNPLFGIIEGWHNL
                     FLRGHVDWGLYLVSLLQSLVIFGIGFALFKKLSWKFAEVL"
     gene            complement(1718..2836)
                     /locus_tag="P5573_06100"
     CDS             complement(1718..2836)
                     /locus_tag="P5573_06100"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRT27668.1"
                     /translation="MRVLQFGKFYPPDIGGIETAICGMTEELNKRGVRCDVLCSNSAN
                     EYREETINGYKVYRTKSYGKVASTSISPQMVFKLREIVGRYDVIHVHHPDPMANLALF
                     LVNPKRQKVVVHWHSDIVNHRFLLQLYKPLLLWMLKRADAVVVASPLYIEGSPFLQKF
                     RAKVEIAPYEVERLAHDEEKVKEIKSKFAGKKIVFSLGRFVYYKGFEYLIEAAEYLDD
                     RYVVLLGGAGPEEEKLRPLAEEHKNRVFLLGEISRKDLGSYYKACDVFCLPSVERSEA
                     FGIAMVEAMSFGKPVVATKIPHSGVQWVNRHKVTGLNVEPKNPKALAYAIRQILENKE
                     LYRRMSENALKRYREKFSVEKEIDDSRPAVSSVVSSAV"
     gene            complement(2833..>3225)
                     /locus_tag="P5573_06105"
     CDS             complement(2833..>3225)
                     /locus_tag="P5573_06105"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRT27669.1"
                     /translation="LDAERDWGYAPEYVEGMWLMLQHGEPDDYVLATGETHSVREFVE
                     KAAEVAGFDIRWEGKGTETRGIDRRSGKVVVEVSPEFYRPAEVGVLVGNPEKAMEKLG
                     WRPRTHFEELVRIMMEADLGRVSQESKR"
BASE COUNT          757 a          920 c          783 g          765 t
ORIGIN      
        1 agtatttccg ggtcgagatg ggcgaccaca gagaacccca gccgcgtcac catccccgag
       61 gagtaggtcc tgagcggctg gtcgatgaac tcttcgagtt cggaaaaccc tatgatttcg
      121 tccatcttct ggacaaccct ctttctcgtc atccccagca acaccccgtt caggactatg
      181 ttttccctgc ccgtgagctc gggatgaaac ccggccccca actcgagcaa gggagagacc
      241 ctgcccttta tctcgatggt gcccatgtcg ggtttcaatg cccccgcggc gagccctaag
      301 agggtgcttt tcccggcgcc gttattcccg atgatcccaa aacattcccc cccgtagact
      361 tcgaaggaaa tctctttgag ggcggtaaaa ctctttttgg tgtggtttat ggcctcggga
      421 agattgaaaa gaaacatctt caggccggcg gttatgtaac cgtagtaagg gtaggtcttc
      481 gtcacaccgt cgaagacgat aacaggctct ttcaaaggac ctccgcaaac ttccaagaaa
      541 gcttcttgaa taacgcaaac ccgatgccga agatgactaa ggactgcaga agagaaacga
      601 ggtaaagacc ccagtccaca tgcccccgca agaagagatt gtgccagcct tcgatgatcc
      661 cgaagagtgg gttcaacaaa aaatactgcc tatacctctc ggggacgagg cttatgtcgt
      721 agatgatggg tgtcatgtaa aagaggacgg tcaggccgat catcacaagt ctttccaggt
      781 cccggaagaa aaggttgagt gcagatacgg cgagggcgca tccgtaggtt atgaagaatt
      841 gggataccat caagagggga ataccgtaga cccatgccgc cgtcaggtgg accttgtaga
      901 agaccacaaa ggcgaagacg atgggaagag acacgacgaa atgaaaggtg tcgttgagaa
      961 ccgtggtgag ggggatgaag tatctgggga aactgacttt ctttacgagt gatgcgttac
     1021 cgaaaagcgc cgtgatggag gcattgacag aggtcgaaaa ccactgccac gggaagagcc
     1081 ccgagattaa aaagagggtg aagttctcta caggcacctt catcacgaac ttgaaggcaa
     1141 aatagaaaac caccgccaag cacagggggt tcaggacgga ccagacatag ccaagaatac
     1201 tgtttttgta tttgactttt atctcctttg ccgtcaagac gagaatcagg tctctgtaat
     1261 aggcaaaatt ctgtacaagg gattccataa gtccaatcca tccatgattt cgagtccttc
     1321 gtcttcgccg ctctcgtaag aagccacaat aacgtcatgc cggacctgat ccggcatcca
     1381 gaatatatag aaaacgctgg ataccggctt ccgccggtat gacgacgtga gctggacacc
     1441 gccccgattt gcatcggggt gacgcgccgt tatgacggac ggcatttttc agtgccccgg
     1501 gatactatca cggaacgccc gaatctgcaa aatcatcggg ctgtttcggc gacaaatccg
     1561 atgggctcgc aagaagtcca gaattaccac tttcgtcatt ccggcgaacg ccggaatcca
     1621 gttaattcaa tatgttctgg atgccggatc aggtccggca tgacgttttt ggacttttta
     1681 cgagggcgac aaatctgaca aatctgattg atgggattca tactgcgctt gatacgacgg
     1741 aagatacggc aggcctactg tcgtcgatct ctttttctac gctgaacttc tccctgtacc
     1801 tcttcagggc gttctccgac atcctcctgt aaagttcttt gttctccagt atctgcctta
     1861 tagcataggc gagggccttg gggttcttag gctccacgtt caggcccgta accttatgcc
     1921 ggttgaccca ttggacgccg gaatggggga ttttagtggc caccacgggc tttccaaagc
     1981 tcatggcctc gaccatcgcg atcccgaagg cttcggacct ttcgacggaa ggcaagcaga
     2041 agacgtcgca agccttgtag taactcccca ggtcttttcg ggaaatctcg cctaaaagaa
     2101 acaccctgtt cttgtgttcc tcggccagag gcctcaattt ttcctcttcc ggccctgccc
     2161 cacccaggag caccacatac ctgtcgtcta agtactccgc cgcctctatc aggtattcga
     2221 agcccttgta gtagacgaac ctccccaggg aaaacactat cttcttgccc gcaaacttac
     2281 tttttatctc tttgaccttt tcttcgtcat gggccaacct ttcgacctca taaggggcta
     2341 tctcgacctt ggccctgaac ttctgcagaa aaggagaccc ctcgatgtac aggggcgacg
     2401 caaccacgac agcatccgcc ctcttcaaca tccaaagaag caaaggctta tagagctgga
     2461 gcaaaaacct gtggtttacg atgtcactat gccaatggac gacgaccttc tgccttttcg
     2521 gattgaccaa aaacaaggcc aggttcgcca tcgggtctgg gtggtggaca tggattacgt
     2581 cataccggcc gactatctct ctcagcttga agaccatctg tggactgatg gaggtcgagg
     2641 ctaccttgcc gtaactcttc gtcctgtaaa ccttatagcc gtttatcgtt tcctctctgt
     2701 attcgttggc gctgttggag caaaggacgt cgcacctcac gccccttttg ttgagctcct
     2761 ctgtcattcc acagatagcc gtttctatcc caccgatgtc cggcgggtag aatttaccga
     2821 actgcaggac cctcatctct tgctttcctg tgacaccctt cccaagtccg cttccatcat
     2881 gattcttacc agttcttcga aatgtgtccg cggcctccat ccgagctttt ccatcgcctt
     2941 ctccgggttc ccgaccagga cgccgacttc cgccggcctg tagaattcag gagaaacctc
     3001 gacgaccacc ttgcctgacc ttctgtctat ccccctggtt tcggtccctt tcccctccca
     3061 gcgtatgtcg aacccagcta cctccgccgc cttctccacg aattcccgca ccgagtgggt
     3121 ctctcccgtc gcaaggacat aatcgtccgg ctcaccatgc tgtaacatca accacatgcc
     3181 ttctacgtat tccggcgcgt acccccagtc tctctcggcg tccag
//
LOCUS       DAOTMX010000191         3224 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_23514_length_3224_cov_3.216788, whole genome shotgun sequence.
ACCESSION   DAOTMX010000191 DAOTMX010000000
VERSION     DAOTMX010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23514_length_3224_cov_3.216788"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="P5573_06110"
     CDS             <1..338
                     /locus_tag="P5573_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27670.1"
                     /translation="GTKKTIVRTGSVYAGLGKSDGAAITLIPLLGEKPGVRSLLLGHV
                     TFNEALSVGEKIATLGDRYNDIKNLVNEYNLPWDDGYLEKLPIGILLGESVEFIATKI
                     KKTLKETDQ"
     gene            335..1873
                     /gene="guaA"
                     /locus_tag="P5573_06115"
     CDS             335..1873
                     /gene="guaA"
                     /locus_tag="P5573_06115"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HRT27671.1"
                     /translation="MIAILDFGSQYNQLIARRVRESRVYCEILPHDIPLDELAAKKPR
                     GIILSGGPASIFDEGVPKSRPALFDLGLPVLGICYGMQLIVHQLGGTVEPSSVREYGR
                     ADLLLTPKGKESPLFTGLAERQRIWMSHGDRVAKLPPGFEVIGSTPNSPVAATENRAR
                     SIYGVQFHPEVVHTEKGNEIIRNFLFRVAGCSPDWTMRSFVEETLVRLREQIGKERVL
                     CALSGGVDSSVLATLLHRAVGERLVSVFVDNGLLREGEAEEVVRTFRDQYHLNFKIIR
                     ARERFLRKLAGVTDPERKRKVIGNEFIRLFEEEARKLGKIKFLAQGTLYPDVIESRSS
                     FGGPSAVIKSHHNVGGLPEKLDMELVEPLKVLFKDEVREVGRELGLPERIINRHPFPG
                     PGLAVRVIGKVTRKRLETVRKADAIVREEVVKAGLFDEVWQEFAVLLPVRTVGVMGDG
                     RTYENVIAVRAITSTDGMTADWVKFPHEVLETISSRIVNEVKGVNRVVYDISTKPPST
                     VEWE"
     gene            1896..>3224
                     /locus_tag="P5573_06120"
     CDS             1896..>3224
                     /locus_tag="P5573_06120"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HRT27672.1"
                     /translation="MRRTKFIFVTGGVLSSLGKGLAAASIGCLLESRGLKVTHQKLDP
                     YINVDPGTMNPFQHGEVFVTDDGAETDLDLGHYERFTSATLGKANNLTTGQVYYSVIK
                     KERRGEYLGGTVQVIPHITNEIKDYIHKAAEGADVAIVEVGGTVGDIESLPFLEAVRQ
                     FRNEVGRENAIFIHLTWVPYIKTAGEVKTKPTQHSVAALREIGIQPDILLCRAENILS
                     QEIKAKISLFCNVEIEAVFTARDVSHIYEVPLNFNEEGVDQKIVNLLNIWTGQPHLDG
                     WRTMVERLKNPHHEVSIAIVGKYVNLVDSYKSLSEALAHGGIANDSRVHLRYVDSERV
                     EHEGLDRLLDGIDGILVPGGFGQRGIEGMIRAVEHARRNRIPFFGICLGMQMAVVEFA
                     RNVCGLERANSTEFDEKTPHPVIDILPEQRHITDRGATMRLGSYPCVLENS"
BASE COUNT          713 a         1013 c          985 g          513 t
ORIGIN      
        1 tgggaacaaa aaagaccatc gtgaggacgg ggagcgttta cgccgggctg ggcaaatccg
       61 acggcgccgc catcacgctg atcccgctct tgggcgaaaa acccggggtg agaagcctcc
      121 tcctcggcca tgtcaccttc aatgaagccc tctccgtcgg ggaaaagata gcgacccttg
      181 gggaccgtta caacgacatc aagaacctgg taaacgagta caacctcccc tgggacgacg
      241 gctatctcga aaaattgccc atcggaatac tcctgggaga gtccgtggag ttcatcgcga
      301 ccaagatcaa gaagaccctg aaagaaaccg accaatgatc gccatcctcg actttggctc
      361 tcagtacaac caactgatcg cccggcgcgt ccgtgaaagc cgcgtctact gcgaaatcct
      421 gccccacgac atccccctcg acgagctggc ggcaaaaaag ccccggggga tcatcctctc
      481 cggcgggccg gccagcatct tcgatgaggg ggtgccgaag agccgaccgg cgctcttcga
      541 cctcggcctc ccggtcctcg gtatctgcta cgggatgcag ttgatagtcc atcaactggg
      601 gggcaccgtc gagccgtcct cggtacggga atacggccgg gcggacctgc tccttacgcc
      661 gaaaggcaag gaatcgccgc tcttcacggg cctcgcggag aggcagagga tctggatgag
      721 ccacggcgac agggtagcaa aactcccccc cggcttcgag gtcatcggct cgacacccaa
      781 ctctcccgtt gccgcgacgg aaaaccgggc aaggagcatc tacggcgtcc agttccaccc
      841 ggaggtggtc cacacggaga aggggaacga gatcatcagg aatttcctct tccgggtcgc
      901 cggctgcagt cccgactgga cgatgcgctc cttcgtcgaa gagaccctcg ttagactgag
      961 ggaacagatc ggtaaagaac gggtcctctg cgccctgagc ggcggggtgg actcgtcggt
     1021 cctcgcaacg ctcctccaca gggccgtggg cgagagactc gtctccgtct tcgtggacaa
     1081 cggcctcctg cgggagggag aggccgaaga ggtcgtccgg accttccggg accagtacca
     1141 cttgaacttc aagatcatcc gtgcccggga gcgtttcctg cggaagctcg ccggggtgac
     1201 ggacccggaa aggaaaagga aggtcatcgg caacgagttc atccgtctct tcgaggaaga
     1261 ggcaaggaaa ctgggaaaga taaaattcct cgcccagggg acgctttacc ccgacgtgat
     1321 cgagagccgg tcgtccttcg gcggaccgtc cgccgtgatc aagagccacc acaacgtcgg
     1381 cggccttccc gaaaaactgg acatggaact cgtggagccg ctgaaggtgc tcttcaagga
     1441 cgaggtccgc gaggtcggca gggaactggg gctcccggaa aggatcatca acagacaccc
     1501 cttccccggc cccggccttg ccgtccgggt gatcgggaag gtcacgcgga agcgcctcga
     1561 gaccgtacgg aaggccgacg ccatcgtccg ggaagaggtg gtaaaggccg ggttgttcga
     1621 cgaagtctgg caggagttcg ccgtcctcct gcccgtaagg accgtcgggg tcatgggcga
     1681 cggccggacc tacgaaaacg tcatcgccgt cagggcgatc acgagcacgg acgggatgac
     1741 ggccgactgg gtcaagttcc cccacgaagt cctggagacc atctccagca ggatcgttaa
     1801 cgaggtcaaa ggcgtcaacc gcgtggtcta tgacataagc accaaacccc cgagcaccgt
     1861 agagtgggag taagggagta gaaaagagcg caggcatgag aagaactaaa ttcatcttcg
     1921 tgacgggcgg cgtcctctct tcgctcggca aaggactcgc ggcggcgtcc atcggctgcc
     1981 tcctcgaatc ccggggtctg aaggtcaccc atcaaaaact cgacccttac ataaacgtcg
     2041 accccgggac gatgaacccc ttccagcacg gagaggtctt cgtgaccgat gacggcgccg
     2101 agacggacct cgacctcggc cattacgagc ggttcaccag tgcgaccctg ggtaaggcga
     2161 acaacctcac taccggccag gtctactact ccgtcatcaa gaaagagcgt cggggagaat
     2221 acctcggggg caccgtccag gtcattcccc acatcacgaa cgagatcaag gattacatcc
     2281 acaaggccgc cgaaggggcc gacgtggcca tcgtcgaggt aggcgggacg gtgggggaca
     2341 tcgaaagcct ccccttcctc gaggcggtcc ggcagttccg aaacgaggtc ggccgggaaa
     2401 acgccatctt catccacctg acctgggtcc cctacatcaa gacggccggc gaggtgaaga
     2461 ccaagcccac ccagcacagc gtcgccgcgc tgcgggagat cggtatccag cccgatatcc
     2521 tgctctgtcg ggcggaaaac atcctctccc aggagataaa ggccaagatt tccctcttct
     2581 gcaacgtgga gatcgaggcc gtcttcaccg cccgggacgt ctcccacatc tacgaggtcc
     2641 cgctcaactt caacgaagaa ggagtggacc agaagatcgt caacctgctc aacatctgga
     2701 cgggccagcc gcacctggat ggttggcgca ccatggtcga gcggctgaag aacccgcatc
     2761 acgaggtctc catcgccatc gtcggcaagt acgtgaacct ggtcgactct tacaagagcc
     2821 tcagcgaggc cctcgcccac ggcgggatag ccaacgacag ccgggttcat ctgcgctacg
     2881 tggactcgga gagggtggag catgaaggcc tggaccgtct gttggacggc atcgacggta
     2941 tcctcgtccc cggcgggttc ggccagcgcg gcatcgaggg gatgatcagg gccgtcgaac
     3001 acgccaggcg aaaccgcatc cccttcttcg gcatctgcct gggcatgcag atggccgtcg
     3061 tggagttcgc ccggaacgtc tgcggcctgg agcgggcgaa cagcacggag ttcgacgaaa
     3121 aaacccccca cccggtcatc gacatcctgc cggagcagcg acacatcaca gacagggggg
     3181 cgacgatgcg cctggggtct tacccctgcg tcctcgaaaa cagc
//
LOCUS       DAOTMX010000192         3221 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_23538_length_3221_cov_4.060328, whole genome shotgun sequence.
ACCESSION   DAOTMX010000192 DAOTMX010000000
VERSION     DAOTMX010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23538_length_3221_cov_4.060328"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /gene="ruvB"
                     /locus_tag="P5573_06125"
     CDS             complement(<1..890)
                     /gene="ruvB"
                     /locus_tag="P5573_06125"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HRT27673.1"
                     /translation="MPKMKDPTISPKKIEDENSYEATLRPKRLDDYIGQEKVKENLSI
                     FVEAARNRGEALDHMLLYGPPGLGKTTLAYIAAREMAVDIKVSSGPVIERPGDLVAVL
                     TNLHEGDVLFIDEIHRLPHVVEEILYPAMEDYHIDIMIGQGPSARTMKLEIPKFTLVG
                     ATTRAGLLTSPLRDRFGMSFRLEYYSPEELAVIVKRSARILSVPIDSGGAGEIASRSR
                     GTPRIANRLLKRVRDYAQVRAEGLIDSSVACKALQMFDVDDKGFDHMDRKILLTLIDK
                     FGGGPVGIESLSSAVGEERDT"
     gene            complement(895..1506)
                     /gene="ruvA"
                     /locus_tag="P5573_06130"
     CDS             complement(895..1506)
                     /gene="ruvA"
                     /locus_tag="P5573_06130"
                     /inference="COORDINATES: protein motif:HMM:NF001040.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HRT27674.1"
                     /translation="MIASLQGTLTHKSLSGIIVEVNGIGYQVFVPLSTYYELPEVGLP
                     VSLKIHTYVKEDALQLFGFRTEEEKDVFQLMISVTGIGPRLATNILSGISALEFVRAV
                     CNDDVKRLVGIPGVGRKTADRMVFELKDRLSKMVPPQDAVAGEGKAVDGWMKEDALSA
                     LVNLGYRGNVAKEAVDRVVDGAPEGLTLEVTLKRALKILAKQQ"
     gene            complement(1511..1996)
                     /gene="ruvC"
                     /locus_tag="P5573_06135"
     CDS             complement(1511..1996)
                     /gene="ruvC"
                     /locus_tag="P5573_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HRT27675.1"
                     /translation="MVMRILGIDPGTNVTGYGMIEKNRSGLSEILHGEIRLSRNQRMT
                     KRLLKTYEDLTALIEEAKPDIVAVEDIFYGKNVKSLIKQGHARGVVLLAASQYNLPVY
                     EYSPLEIKKAVVGYGRAEKIQVQRMVKAILGMSALPPEDAADALAVAVCHSHTGKTKN
                     I"
     gene            complement(2033..2782)
                     /locus_tag="P5573_06140"
     CDS             complement(2033..2782)
                     /locus_tag="P5573_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRT27676.1"
                     /translation="MSGHSKWATIKRKKGVADARRGKIFTKLIKEITLAARLGGGDPE
                     GNARLRQAILAAKAENMPKDNIDRAIKKGAGELGGASAYEELTYEGYGPGGVAVLVDV
                     MTDNKKRTVSEIRHIFSKYGGNLAENGAVAWLFEKKGSILVEKKGIDEDKLIEVALEA
                     GAQDVREEENEYEIITEPASFDAVKKAVDTAGFKCIQAQISMIPRNTVKLNDDRAGQM
                     LKLMEKLEDNDDVQNVYANFDIPDEVMERLG"
     gene            complement(2913..3029)
                     /gene="rrf"
                     /locus_tag="P5573_06145"
     rRNA            complement(2913..3029)
                     /gene="rrf"
                     /locus_tag="P5573_06145"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(3106..>3221)
                     /locus_tag="P5573_06150"
     rRNA            complement(3106..>3221)
                     /locus_tag="P5573_06150"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          645 a          980 c          788 g          808 t
ORIGIN      
        1 gtgtccctct cttcaccaac ggcggaagag agactttcta tgcccaccgg tccgccgccg
       61 aacttgtcga tcaaggtcaa cagtatctta cgatccatat ggtcgaaacc tttgtcgtcg
      121 acgtcgaaca tctggagtgc cttgcaggcc acggaactgt ctatcaggcc ttcggccctg
      181 acctgggcat agtcccgcac ccgtttcagc agacgattcg cgatgcgcgg cgtaccccgc
      241 gaacggctcg ctatttcacc ggcccctccg gagtcgatgg gaaccgatag gatccgcgcc
      301 gaccgtttga cgatgacggc cagttcctcc ggagagtaat actccaacct gaaactcatt
      361 ccgaaccggt cccggagcgg agaggtcagg aggcccgccc tcgtagtcgc cccgacgagg
      421 gtgaatttcg gaatctccag tttcatcgtc ctcgccgacg gcccctgacc gatcatgatg
      481 tcgatatgat aatcctccat ggccggatac aggatttcct ctacgacgtg ggggagacgg
      541 tggatctcgt cgatgaacag cacgtccccc tcgtggaggt tcgtcaggac ggcaacaagg
      601 tcccccggac gctcgatgac cgggccggag gaaaccttga tatccacagc catctcccgg
      661 gccgctatgt aggcaagcgt cgtcttaccc aggcccggcg ggccgtagag gagcatatgg
      721 tcaagggctt cgcccctgtt tcgtgccgct tccacaaata tggacaggtt ttctttgacc
      781 ttttcctgcc ctatgtagtc gtccagtctc ttcggcctaa gggttgcctc gtagctgttc
      841 tcgtcttcga ttttcttcgg gctgatggta gggtctttca tcttcggcat acgctcattg
      901 ctgttttgcc aggatcttca gcgccctctt cagggtgact tccagggtga ggccttcggg
      961 tgccccatcg accacccggt ccacggcttc tttcgctacg ttccccctgt acccaaggtt
     1021 gacgagcgcg gacagggcgt cctctttcat ccatccgtcg acggccttac cttcgcctgc
     1081 aacagcgtct tgcgggggaa ccatcttgga tagcctatcc ttcaactcga agaccatgcg
     1141 gtccgcggtc ttcctgccga cccccggtat gccgacgagc ctttttacgt cgtcattgca
     1201 gacggcccgg acaaattcca gggcagatat gcccgaaagg atgttggtcg ccagtctcgg
     1261 tccgatgccc gtcaccgaga tcatcagctg aaaaacatcc ttctcttcct cagtccgaaa
     1321 cccgaaaagc tgaagagcgt cttctttcac gtatgtatgg attttcaggg acacgggcaa
     1381 cccgacctcg ggcaactcgt aataggtgct cagagggaca aagacctggt aaccgatgcc
     1441 gttgacctcg acgattatgc ccgaaagcga tttgtgggtg agcgttccct gcaggctcgc
     1501 gatcatcgtc tcaaatgttt ttcgtcttcc cggtgtgcga gtggcagacg gcaactgcaa
     1561 gggcgtccgc ggcgtcctcc ggaggaaggg ccgacatccc gagaatcgcc ttgaccatcc
     1621 gctgaacctg tatcttttcc gctcggccgt aaccgacgac cgcttttttt atctctaggg
     1681 gagagtactc atacacgggc aggttatact gggaagcggc caggaggaca acgccccgtg
     1741 cgtgtccctg ctttatcaag ctcttcacgt tcttcccgta gaaaatatct tccacggcaa
     1801 cgatgtcggg cttcgcttcc tcgatcaggg ccgtcaagtc ttcgtaggtc ttcagcaacc
     1861 gcttcgtcat cctttggttc cgggaaagcc tgatctcgcc gtgcaatatc tcagatagcc
     1921 cactgcggtt tttttctatc atcccataac cggtcacgtt cgtgccgggg tcgatgccca
     1981 agattctcat caccatgctc tcagagctcg ggagaagatt cccgccggcg gcctaaccga
     2041 gcctctccat gacctcgtca ggaatgtcga aattggcata aacattctgc acgtcgtcgt
     2101 tatcctccag cttttccatc aatttgagca tctgacccgc cctgtcatca ttcagcttaa
     2161 ccgtgttccg gggaatcatg cttatctgcg cctggataca tttaaagccg gccgtatcga
     2221 cagccttttt gacggcatcg aaggaggcgg gttcagtgat gatctcgtac tcgttttctt
     2281 cttcccgcac gtcctgcgcc ccggcttcaa gggccacctc gatcagcttg tcctcatcta
     2341 tccccttctt ttcgaccaga atacttcctt tcttctcgaa aagccacgcg acagcgccgt
     2401 tttccgccag gtttccgcca tacttcgaga agatatgccg gatttcggag acggtccttt
     2461 ttttattatc ggtcatgacg tccacaagaa cggcaactcc tcccggaccg tatccttcat
     2521 atgtgagttc ttcgtaggcc gacgccccgc cgagttcgcc ggcccctttc tttatggccc
     2581 tgtctatatt gtctttgggc atgttctcgg ccttagccgc caggatagcc tgccgcagcc
     2641 ttgcattgcc ttcggggtca ccacccccca aacgggcggc aagggtgatt tccttgatca
     2701 attttgtgaa tatctttccg cgcctcgcat cggcgacgcc ttttttccgc tttatcgtag
     2761 cccatttcga atgacctgac atacaaagta atactcctta ttgactctcg gcattcaact
     2821 cgaaaatccg gttgacaggt ttttaacaca gtgtctttcc atttgcatgc aaaaaaaaac
     2881 ccctccgtca acgaaggggt tcattttttt attttcggcg acgtcctact ctcccacaca
     2941 gtcacccatg cagtaccatc ggcgctggag agcttaactt ccgtgttcgg gatgggaacg
     3001 ggtggatcct ctccgccata gtcaccgaaa aaactaaaga ctgaacttca agacaactgc
     3061 ataccgtgaa tacgatgaaa gcttcataaa ggtcattatt aaattatggt caagccgcac
     3121 gaccgattag tatcagtaag ctgaacacat tactgtgctt acacacctga cctatcaacc
     3181 tcgtggtcta cgaggggtct tcagtcctga tgtctccacc a
//
LOCUS       DAOTMX010000193         3220 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_23556_length_3220_cov_4.116272, whole genome shotgun sequence.
ACCESSION   DAOTMX010000193 DAOTMX010000000
VERSION     DAOTMX010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23556_length_3220_cov_4.116272"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="P5573_06155"
     CDS             <1..441
                     /locus_tag="P5573_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecD/SecF family protein translocase subunit"
                     /protein_id="HRT27677.1"
                     /translation="YYRYSGIVADMALFLNVILTLGTLAIFKATLTLPGIAGILLTIG
                     MAVDANVLIFERAREEIRLGKTPRAVIETGYGKAFITILDTHVTTLIAALFLFQFGTG
                     PIKGFAVTLTIGLIYSVFTAVFVTRIVFDYFIWNRKVKRISIGI"
     gene            530..1450
                     /gene="secF"
                     /locus_tag="P5573_06160"
     CDS             530..1450
                     /gene="secF"
                     /locus_tag="P5573_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HRT27678.1"
                     /translation="MELIKPGTNIDFVGKMRIFLYISVSLVIMSILYLILIGGPRLGI
                     DFAGGTSVQVKFNQATTVEKIRDALKSINMESSIIQQVGAKSDNEFLLRTEISTSDLK
                     GLQDKIEGALAAAYGKGSFEMRKVEVVGPKAGKDLRQKGILALCLALLGMLAYITWRY
                     EFRYALGGIVALVHDVIVTVGVFALLGREFTLTVIAAILTIIGYSINDTIVVFDRIRE
                     NVKKNPRQNLGEVMNFSVNQTLSRTILTSFTVFLVVLSLYLFGGSVINDFAFILLVGV
                     VVGTYSSIFIASQFVLVWESFRLSKKARKK"
     gene            1645..2256
                     /gene="lepB"
                     /locus_tag="P5573_06165"
     CDS             1645..2256
                     /gene="lepB"
                     /locus_tag="P5573_06165"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRT27679.1"
                     /translation="MGGSRHKIKEYAEAIVIAILIALFIRTFVVQAFKIPSGSMKPTL
                     LVGDHILVNKFIYGVKIPYIQKTVVSISEPKRGDVVVFIYPEDRSKDFIKRVIGVGGD
                     TIEIRNKQIFLNGQPYQDPFGLYVDSVIIPGSMQPRDNYGPVRVPEGMLFVMGDNRDQ
                     SYDSRFWGFVDLKDVMGKAFIIYWSWDSSEMTVRWNRIGKIIH"
     gene            2444..2998
                     /gene="pyrR"
                     /locus_tag="P5573_06170"
     CDS             2444..2998
                     /gene="pyrR"
                     /locus_tag="P5573_06170"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HRT27680.1"
                     /translation="MSKRKVVMDAEGIDRALTRIAYEILEKNKGVENLALVGIRTGGV
                     FLARRIAEKISQIEGKEVPLGILDITLYRDDLRKNRKKPLIGKTDIPFSLDDRKVVLI
                     DDVLFTGRTVRAAMDALIDFGRPKAIQLAVLVDRGHRELPIRADFVGKNLPSSLWEDV
                     SVNLTENKGKDEVVIEEEGKSDTA"
     gene            3022..>3220
                     /locus_tag="P5573_06175"
     CDS             3022..>3220
                     /locus_tag="P5573_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041585001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HRT27681.1"
                     /translation="MKLTSKDILGMKDLSVDEIELILETAESFMEISTREIKKVPTLR
                     GKTVVNLFFEPSTRTRTSFEIA"
BASE COUNT          785 a          747 c          916 g          772 t
ORIGIN      
        1 tactaccggt attcgggaat cgtcgccgat atggcgctct ttttgaacgt catcctgaca
       61 ctggggaccc tggctatttt caaggccact ctcaccctcc cgggtattgc ggggatcctg
      121 cttacgatcg gcatggccgt cgacgccaac gtcttgatct ttgaacgggc gagagaagag
      181 atacggctgg gcaagacacc gagggccgtc atagagacag gctatggcaa ggccttcatt
      241 accatcttgg acacacacgt tacgacgttg attgcagcgc tctttctgtt ccagttcggg
      301 accggtccca tcaagggatt cgcggtgaca ctgacgatag ggctcatcta cagcgtcttc
      361 accgccgttt ttgtgactcg tatcgtgttc gattatttca tctggaacag gaaggttaag
      421 cgaataagca taggaatata gatatggggg agaggcaaag agcagagggc ggaaccaaga
      481 tttctcggcc ttctgccttt catccttgtc ttcgatcgag gagcatcgaa tggaactgat
      541 aaagcctggg accaacatcg atttcgtcgg taagatgcgg atatttcttt atatttccgt
      601 atcgttagtg attatgagca ttctgtacct tatcctgatc ggaggtccga ggctggggat
      661 agacttcgcc gggggtacgt cggtccaggt caagttcaac caggcgacga cggtcgaaaa
      721 gatcagggat gcattgaagt cgatcaacat ggaaagcagc atcattcaac aggtcggtgc
      781 aaagagcgat aacgagtttc ttctcaggac ggaaatttcc acgtcggacc tgaaggggct
      841 gcaggataaa atcgagggtg ccttggccgc cgcatacggg aagggcagtt tcgagatgag
      901 aaaagtcgaa gtcgtggggc ccaaggcggg taaagacctc cggcagaagg gcatcttggc
      961 gctttgctta gcccttctgg ggatgctcgc ctacattacc tggcggtatg aattccggta
     1021 cgccctcggc gggatcgtcg cgctggttca cgatgtcatc gtgaccgtcg gggtcttcgc
     1081 tctgttgggc cgggagttca cactgacggt catagcggct atcctgacca tcataggcta
     1141 ttcgatcaac gatactatcg ttgtcttcga cagaatcaga gagaacgtaa agaagaatcc
     1201 gagacaaaac ctgggcgagg tcatgaattt cagtgtaaat cagacactga gcaggacgat
     1261 tcttacttcc ttcacagtgt tcctggttgt actctcactc tatttgtttg gagggtcggt
     1321 gataaacgat ttcgccttca tcctgcttgt gggggttgtt gtcggaactt attcgtccat
     1381 tttcatcgcg agccagttcg tgttggtctg ggaatccttc aggctgtcaa aaaaggcaag
     1441 gaaaaagtga tatcggccat tgtcgtatcg gcgggggttg acggttccga ttcctgcctt
     1501 ttttcgataa tttttcccga cctttacggt tctctgagtg cgaacgcaat tcttcgtgcg
     1561 ggcgttgtcg ctcatttgcc gaaggatgca gcaggctgac agccaccctg ctattgccgg
     1621 cattatatcc ctgaaaggaa agctatgggc ggttctcggc acaagatcaa agaatacgca
     1681 gaagcgatcg taatcgccat cctcatcgcg ctctttataa gaaccttcgt tgtgcaggcc
     1741 ttcaagatac cgtcgggttc gatgaagccg acgctcctcg tgggggacca catcctcgtg
     1801 aacaagttca tctacggcgt gaaaatcccc tatattcaga aaacggtcgt ctcgataagt
     1861 gaaccgaaac gaggagatgt cgtcgtgttc atctatcctg aggataggtc aaaggacttt
     1921 atcaagagag tcatcggtgt cgggggcgac acaatcgaaa tccgtaataa acagattttt
     1981 ctcaacggtc agccttacca agaccccttc ggcctttatg ttgacagcgt catcataccc
     2041 gggtccatgc aaccccgcga taattacggt cccgttcggg tgccggaagg aatgctgttc
     2101 gtcatggggg ataaccgtga tcagagctat gacagccggt tctgggggtt tgtggaccta
     2161 aaagatgtga tgggcaaggc ctttataatc tattggtcgt gggatagttc tgagatgacc
     2221 gtccggtgga acaggatcgg caaaatcatt cattgattcc ttgacttgaa cggtcgtgtc
     2281 tgatattttt tacaggaaaa aagcgacaga gcccttttaa ttcgatccgg tgaggttaaa
     2341 aagggagcga tggtaaactc ggtgggtgaa tgatgattga gggccttctc atgaagtggg
     2401 aaggcctttt tcgtttttat ctccccgcgc ggaggtggtc gagatgagca agagaaaggt
     2461 ggtcatggat gccgaaggga ttgaccgggc actgacccgt attgcttatg agatcctcga
     2521 aaagaacaag ggggtggaaa atcttgccct ggtggggata aggaccggcg gtgtgtttct
     2581 cgcgcgacgt atcgccgaaa agatatccca gatcgaaggt aaagaggttc cgctggggat
     2641 actcgacatc acgctctacc gcgatgacct gagaaagaac cggaaaaaac ctctgatcgg
     2701 gaagacagac atcccctttt ctctggacga caggaaagtg gttctcatcg acgatgtcct
     2761 cttcacgggt aggacggtga gggcggcaat ggatgccctg atcgatttcg gcaggccgaa
     2821 agcgattcag ttggccgtcc tcgtcgacag gggccatagg gagctgccca taagggccga
     2881 tttcgtgggg aagaacctgc cttcctccct gtgggaggat gtgagcgtca acctcacaga
     2941 aaacaaggga aaagacgaag tcgtgatcga agaagaggga aagtcagaca cggcgtaaca
     3001 gttaacctga gtgaagacgg catgaaattg acgagcaaag atatcctggg catgaaagac
     3061 ctctccgtcg acgagatcga attgattctc gaaacggccg agtccttcat ggaaatatcg
     3121 acgcgggaga tcaagaaagt gccgaccctt agaggaaaga ccgtcgtgaa tcttttcttt
     3181 gagccgagca caaggacgcg gacatccttt gagatcgcgg
//
LOCUS       DAOTMX010000194         3215 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_23613_length_3215_cov_2.815190, whole genome shotgun sequence.
ACCESSION   DAOTMX010000194 DAOTMX010000000
VERSION     DAOTMX010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23613_length_3215_cov_2.815190"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(916..1056)
                     /locus_tag="P5573_06180"
                     /pseudo
     CDS             complement(916..1056)
                     /locus_tag="P5573_06180"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1082..1423)
                     /locus_tag="P5573_06185"
     CDS             complement(1082..1423)
                     /locus_tag="P5573_06185"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRT27682.1"
                     /translation="MDKESVDELIYNTRSQRDRLIMELQARCGLRIGEVLKIKVSDIS
                     ERKLLIREPKSGKEGEVAFMSEQVARRVDEYIRSKNLSADSRLFPISYSTARSMIIKL
                     GSKLNLKGCAS"
     gene            complement(1738..2781)
                     /locus_tag="P5573_06190"
     CDS             complement(1738..2781)
                     /locus_tag="P5573_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27683.1"
                     /translation="MNKMEALKFLASEHHVVPEELDVTVGYFNPADALGVARLFYAVY
                     GDAYPIDTYYIPERLVEENERGNIRSVVARTSGGDVVSHTALYRSSSPNPNLYEAGLS
                     LTLPSYRSTRAFLGLIPLANALVGHDGIDGYFAENVCNHLVSQKITWNAKAQETALEP
                     ALMPASTYEIEQNADGRVGCIIFFRIARDCRRTLYIPPSYREELAYLLDGLNLDRELV
                     EADVFPAAGGGKLEVRRFDFAGVARCTITSPGTGLAEQLADLERELRTDGYAQIQIFV
                     NLGEQWSGSVVEILRNRGYYLGGLLPVWFGSDGLLMQKHFVDPGFDSLHIYSKRGRYI
                     TDMIRHDWERSRK"
BASE COUNT          733 a          834 c          798 g          850 t
ORIGIN      
        1 atggccgcct tcatcgcccg cgcggaaagc agcagttgcg gaacgaagta ctcgtgggtc
       61 tcgaagcggc gcccgacttc gtccatcgcg accgccatgt agtcgttcac cagccggagc
      121 ggtctgtgtg gctgcaccgt tcgcttcggg ataaaccgca gccgtgggca gaacaggccg
      181 ttcataggta ggagccagcg aacatccggt cacgaccgca aacaggactc caaggaagat
      241 ctttcgatgg gtcattcggt attgcctttc aaagacagag tcacgttatg cagtgaagga
      301 tgagaggtca gtcgcgctca cggggcagct tctcgaagga ccgtttgacc gaatcgatga
      361 tcgaaagttc ccaatctccc atcttccctt tgtcgttatt gaagtaaccg cgcatgctat
      421 caacaatttt cgactctatg ctggattgtt tggactctgt tctccgtcgt agctctttca
      481 catccctgta gttttattat cacaattccc gagtataggt acaaactttt aattacagct
      541 tttaattacc atcctgctgc gcaatacctt agcatctgca attagcggcg gttaagaagc
      601 cttcttccca tgtaataggg cggtgaaaag tcgtgtttcg acgaattgtc cgaagccatt
      661 cgttgagctg catcccccga gtactcttaa ataatggaaa tgtaatatcc ataaattgca
      721 aagagatccc gcctcggctc tgctgattac cgatcgtaat attctgtccg tgcaaaaagc
      781 cattttcaac aacttcacga agtaaggtac cagttaaatc gcaacttccc tttccgttct
      841 caatttctgt aaaatttttg cttgaatttt atagccccaa agacgtctga cttcaatcgg
      901 taaggatgga ccgcactact tggcgtggag tatatccatc catctgattg cctctgactc
      961 gctgactttt ccaagataca cctgggtcgt cttcagatcc tgatgtctca ggatgatctt
     1021 cgatatgatc tccaacggga tgccatttcg gcttgcgtgc gtcgccgagt atctgcgaag
     1081 gtcatgaggc gcaaccttta aggttaagct tgcttccgag tttaatgatc attgacctgg
     1141 ccgttgaata cgatatcgga aagagcctgc tgtcagcgga caagtttttc gatcttatgt
     1201 attcatctac gcgccttgca acttgttctg acatgaaggc aacttcgcct tccttcccgg
     1261 atttcggttc cctaatcaga agctttcttt cagaaatatc ggagaccttg atcttcaaaa
     1321 cctcaccaat tctcaagcca caccgggctt gaagttccat gatgagtctg tctctctggc
     1381 ttcgggtatt gtatatcagc tcatcgactg attctttatc caaaatattt ttggatacct
     1441 gtttaggggc tctaaaagcc ttcgaaaaaa ggggaccatt gcacgggttt ttcatattga
     1501 tcgcgcattt ttcgatgatg aaattacagg tggtcgggtt aaaacgcagg cggccgcaga
     1561 gtgtttctgt cgctgttttc tttgcggttg ctgacggaat accggcaacg gaccgtttcc
     1621 gggggaaaaa acgtggaaga ccggtccgtc tccgggcagg ccgattccag gaacagggtg
     1681 ctgaattccc ggcaccccgt tccaattgtc aagatgaaag atttgacccc tattttccta
     1741 ttttctgctt cgttcccaat catggcgaat catgtctgtt atgtaacggc cccgtttgga
     1801 atagatatgg agggagtcga aacccgggtc cacgaagtgc ttctgcatca acaggccatc
     1861 gcttccgaac cagaccggca ggagcccccc gaggtaatag cccctgttcc gcaatatctc
     1921 cactactgag cctgaccatt gttcccccag attcacgaag atctgaatct gtgcgtaacc
     1981 atccgtacgc agttcccgtt caaggtctgc cagctgttct gcgaggcctg ttcccggtga
     2041 ggtaattgtg caacgggcaa cacctgcgaa gtcaaaccgc cttacctcca gctttccgcc
     2101 gccggcggcg gggaaaacgt cggcctcaac cagttcgcgg tcgagattca ggccgtccag
     2161 cagatacgcc agttcttccc ggtaggaggg cgggatgtac agtgttctgc gacaatcccg
     2221 cgctattcgg aagaaaatga tgcaccccac ccggccatcg gcgttctgct cgatttcata
     2281 ggtgctggcc ggcatgagcg caggctccag tgcggtttcc tgcgccttgg cgttccaggt
     2341 gattttctgg gaaacgaggt ggttgcagac attctccgca aaataaccgt caatcccgtc
     2401 atgtccgacc agggcattcg ccagggggat gaggccgaga aaagccctgg tggagcgata
     2461 ggagggcagg gtcaacgaca accccgcttc gtagagattg gggttcggag aagaactgcg
     2521 ataaagggcg gtgtgggaaa ccacatcgcc accggatgtg cgggcaacaa cgctgcgtat
     2581 attcccacgt tcattctctt ccacaaggcg ctcgggaata tagtaggtgt cgatcggata
     2641 ggcgtcgccg tatacggcgt agaacaggcg ggccaccccc agggcgtcgg cagggttgaa
     2701 atatcctacc gtcacatcca attcttccgg gacgacgtgg tgttcacttg ccaggaactt
     2761 cagtgcttcc attttattca taactcagcc tcctattctc gaaaaaggtt tctttatcgg
     2821 ctgtcacttt tcccattatc cggtcatatg ggattcctgc gatgcgttca ttgccgtatg
     2881 gatcgtgcct cgttagcttg tccgtttctt tgccgctgat ttgatggctg tatcgtcgac
     2941 gatggatttt ctgaactcgt cgaaaaaaca tttttgtctt ttctcatatt gtttcccatg
     3001 tgcgatttta atgcccggct tttacatcga atgggaattc gaatttagca agcacgggaa
     3061 gtcgtgcata caatcaatat cgtccaatcg attgaacagt tgagttgacc tacgagtgcg
     3121 gaatctcttc tccccgagag gtttttttgt gccatatgtg gaccagcgag gccagaacaa
     3181 caagcaggct caaaagaccc aggcgggtaa agagc
//
LOCUS       DAOTMX010000195         3198 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_23771_length_3198_cov_4.274897, whole genome shotgun sequence.
ACCESSION   DAOTMX010000195 DAOTMX010000000
VERSION     DAOTMX010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23771_length_3198_cov_4.274897"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..952
                     /gene="cfa"
                     /locus_tag="P5573_06195"
     CDS             <1..952
                     /gene="cfa"
                     /locus_tag="P5573_06195"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: protein motif:HMM:NF008686.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HRT27684.1"
                     /translation="GWWECDRLDEFFHCILKARLDEKVKVKFTDLPSLLLFFVNKQSR
                     SRSRIVADRHYNLGNDLFQSFLDPYNQYSCAYFDGVDDLNSAQVKKLDLICRKMALKQ
                     GDRVLDIGCGWGGFARYAAENYGCRVTAVNIAREQIAYAKEFCRSLPVEIVECDYRDI
                     RGTFDKIVSIGMFEHVGRKNYREFMNVVYRCLDDEGIFLLQTIGDNESLDRVDDWIDR
                     YIFPNGQLPSIAWIGRAVEGLFVMEDWHNLGPHYDKTLMAWNENFQKAWPDLRSRYDE
                     RFKRMWEYYLLSCAGAFRARRIQLWQIVFTKQGTPQPNCR"
     gene            983..1876
                     /locus_tag="P5573_06200"
     CDS             983..1876
                     /locus_tag="P5573_06200"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRT27685.1"
                     /translation="MLKEIRFGKVVFIPGPYEGRYPFCNSLYIDDKVQAVIDPGSDEG
                     RLRRLQEEKGPVRTVIQSHYHEDHGSFGYLFPSAELCVHKSEAPCYQDIDKVLEYYGL
                     RGTEYEDVWRDILLRRFNYRVRKPTCEFQDGDLMQFGETTLRVIHTPGHSIGHCCFYF
                     PDEGVLYLGDLDMTRFGPWCGDRVSDIDSTVESVRKLMTVPARFFVSAHETGVIEGDL
                     RELAEEYIAVIEEREKKLLAFLDRPRTLDEIVGQWIVYKRPREPRHFFEFGERALMKK
                     HIERLEKRGLLRQQRMRYSLV"
     gene            1964..2182
                     /locus_tag="P5573_06205"
     CDS             1964..2182
                     /locus_tag="P5573_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27686.1"
                     /translation="MVVEKEAFLREVQKKFEEKLRENEISLLEYWKERLDRLSSMKPE
                     GIASLQLEIKGISDMMANRIKVLKRKKE"
     gene            2188..2766
                     /locus_tag="P5573_06210"
     CDS             2188..2766
                     /locus_tag="P5573_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRT27687.1"
                     /translation="MENIVNFIFEVGMLKKTPRTGFQFLGTGCESVAEHLLRTVFIGY
                     ALCKLEPGVDENRVIKMCLFHDLPEARTGDMNYVNKKYVTVDEGKAVEELTEGLFFGS
                     EIKSAIDEFNEKKTRESMLSHDADQLALIYQLKELGDLGNKYSKEWIDFAVRRLYTDT
                     ARGMAEEVLKTDSSQWWFKDKSDWWVNGNNGK"
     gene            2786..>3198
                     /locus_tag="P5573_06215"
     CDS             2786..>3198
                     /locus_tag="P5573_06215"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRT27688.1"
                     /translation="MKNLKIPRICHVVISAFVVLYFITGCTAKNPRLAEVFLERGIVY
                     IQQGQYTAALKELLDAKKQNPDDPTIRYYLGIAYYTKKLNAEAVSEFKEAISLKPGYS
                     EAHNYLGMVYCEMGRWDDAIAEFKKAASNVLYETPA"
BASE COUNT          863 a          706 c          912 g          717 t
ORIGIN      
        1 tggctggtgg gagtgtgatc gactcgacga gttcttccac tgcatcttaa aggcgcggct
       61 cgatgagaaa gtcaaagtaa agttcacaga cctgccgtca ctcctccttt tttttgtcaa
      121 caaacaatcg aggtcgaggt cgcggatcgt cgcggatcgg cactacaacc tcggaaacga
      181 ccttttccaa tctttccttg acccttacaa ccaatacagc tgtgcttatt tcgacggggt
      241 cgacgacctc aattcggcac aggtcaagaa gctcgacctc atctgtcgaa aaatggcgct
      301 caaacagggg gaccgtgtcc tcgacatcgg ctgcggatgg gggggatttg cccgttacgc
      361 cgcagaaaac tacgggtgcc gggtaaccgc agtgaacatc gcccgcgaac agatcgccta
      421 cgcaaaggag ttctgtcgtt cactgccggt ggaaattgtg gaatgcgatt atcgagatat
      481 acggggaacc ttcgataaga tagtttccat aggcatgttc gagcatgtgg gacgaaaaaa
      541 ctacagggaa tttatgaacg tagtataccg ctgtctggat gacgagggta ttttccttct
      601 ccagaccatc ggcgacaacg aatccttgga cagagtcgac gattggatcg acaggtacat
      661 cttcccgaac ggtcaactgc cgagcatcgc ctggatcggc cgggctgttg aggggctgtt
      721 cgtcatggaa gactggcata atctggggcc ccattatgac aagaccctca tggcctggaa
      781 cgagaatttt cagaaggcct ggcctgacct gaggtccagg tatgacgaga gattcaagcg
      841 gatgtgggag tattatctcc tttcctgcgc cggtgctttc cgggcgcggc ggattcaact
      901 ctggcaaatc gtctttacca aacagggtac gccgcaacct aactgtcgat aacaacactt
      961 cgaaggaaaa ggtgggaggg ggatgttgaa ggaaatcaga ttcggaaaag tcgtttttat
     1021 accggggccc tatgaaggac gatacccctt ctgtaattct ctgtacatag atgacaaggt
     1081 acaggccgtc atagacccgg ggtctgacga ggggcggttg aggagactgc aggaggaaaa
     1141 aggccctgta cgaacggtca tccagtcaca ttatcatgag gatcacgggt cgttcggtta
     1201 tcttttcccg agtgccgaat tatgcgtcca taagagcgaa gcaccgtgct accaggacat
     1261 agacaaggtc ctcgagtact acggtcttcg cggtacggag tacgaagatg tatggcgaga
     1321 catactcttg cggcgattca attacagggt acggaaacca acctgtgaat tccaggatgg
     1381 cgacctgatg caattcggcg aaacgacgct gcgcgtcatc catacgcccg gccatagcat
     1441 cggacattgc tgtttctatt tcccggacga gggcgtcctt tatctgggcg acctagacat
     1501 gacacgattt gggccatggt gtggcgaccg agtgtcggac atcgacagca ctgtcgagtc
     1561 cgtgaggaaa ttgatgaccg ttcctgcacg gtttttcgtg tccgcccatg aaacgggcgt
     1621 gatagaaggt gatttaaggg aacttgcgga ggaatatata gcagtgatcg aagaaaggga
     1681 gaaaaaactt cttgccttcc ttgaccggcc gagaacgctc gatgaaatcg tcggccaatg
     1741 gatcgtttac aaaaggccgc gagaacccag gcattttttt gaattcggtg agcgggcgct
     1801 gatgaaaaag cacattgaac gcctcgaaaa aagggggctt ttgagacaac agcggatgag
     1861 atattcgttg gtatgaacac cgacggcctc ggctttaact tcttgcattg cggtgaaaga
     1921 ttgatataga tgtacaacaa ggtgagtccg gagggcgagg cgtatggttg tcgagaagga
     1981 ggccttcctg agggaagtcc agaagaagtt cgaagaaaag ctcagagaga atgagatatc
     2041 ccttttggaa tactggaagg agcgactgga tagattgtca tcgatgaagc cggagggtat
     2101 agcgtcatta cagctagaaa tcaaagggat ttccgacatg atggccaata ggataaaggt
     2161 tctcaaaaga aagaaagaat agtcaaagtg gagaacatag taaatttcat attcgaagtt
     2221 ggcatgttga agaaaacgcc gagaacgggg ttccagtttc tcggcacagg ttgtgagtct
     2281 gtagccgaac acttgctgcg gacggtcttc ataggttatg ccctttgcaa gcttgagccg
     2341 ggcgtcgacg aaaatcgggt gataaagatg tgcctgttcc atgatttgcc ggaggcaagg
     2401 acgggagaca tgaattatgt aaacaagaaa tacgtaactg tcgacgaggg gaaggccgtg
     2461 gaggaactta cggaggggct tttcttcggc agcgaaatca agtccgccat cgatgaattt
     2521 aacgagaaaa aaaccagaga gtcgatgctt tcccacgatg ccgaccaact ggcgttgatc
     2581 tatcagttga aggaattggg cgaccttgga aacaaataca gcaaagagtg gattgacttt
     2641 gcggtcaggc gactttacac cgacaccgcg aggggaatgg cggaggaggt cctaaagacc
     2701 gattcctcgc agtggtggtt taaagacaag agcgactggt gggtaaacgg gaacaacgga
     2761 aaataggcgt ggaggaaagt tcataatgaa aaacctcaag attccaagaa tctgccatgt
     2821 cgtgatttct gcgttcgttg tcctttattt cattacggga tgcacagcca aaaatccacg
     2881 attggccgag gtttttctcg aacgcggaat agtttatatt cagcagggtc agtacaccgc
     2941 cgctttgaaa gagcttctgg acgcgaagaa gcagaatccc gatgacccca cgataaggta
     3001 ttacctcgga atagcttatt acaccaaaaa actgaatgcc gaggcggttt cggaattcaa
     3061 ggaggccatt tctttgaagc cgggttattc cgaggcccat aactatctgg gcatggttta
     3121 ctgtgaaatg gggcggtggg acgacgcgat cgcggagttc aagaaagcgg ctagtaatgt
     3181 actatacgag acaccggc
//
LOCUS       DAOTMX010000196         3195 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_23806_length_3195_cov_3.645223, whole genome shotgun sequence.
ACCESSION   DAOTMX010000196 DAOTMX010000000
VERSION     DAOTMX010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23806_length_3195_cov_3.645223"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="P5573_06220"
     CDS             complement(<1..286)
                     /locus_tag="P5573_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRT27689.1"
                     /translation="MSFNVLIVDDSSSMRAVIRKIISISGFKVGRCYEAANGREALDI
                     LLENWVDVIISDINMPGVSGLDMLDHLKADDLLKDIPVIVVSTEGSDERIR"
     gene            complement(325..1173)
                     /locus_tag="P5573_06225"
     CDS             complement(325..1173)
                     /locus_tag="P5573_06225"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HRT27690.1"
                     /translation="MIVDELLHSIQKIPAFPGTVQRVAEILSHADYSVSELVSVIEYD
                     QSITANLLKMSNAAYFGSRHRVSTVQDAVMFLGQENIIRAVVASGVVSFYRSGRGYGV
                     KGSDLWKHSVGVALMSQILSKKLYSRKDGELFTAALLHDVGKVVLGEYIDRSFEKIAR
                     LVKKEGYAFLTAEKEVFGIDHAELGGKIAERWNFPPKHVEVIACHHRPDQSARERIAP
                     WVVYLADQVCLMMGVGGGSDGLAYWGLKEVVQMFKLRQRDIEECMIQLAQELALAEDI
                     VNVFHG"
     gene            complement(1173..1655)
                     /locus_tag="P5573_06230"
     CDS             complement(1173..1655)
                     /locus_tag="P5573_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HRT27691.1"
                     /translation="MAECIVGISDLKVSNNQEDVLITYALGSCIGVAVYDPQVRVGGL
                     LHFMLPDSNLDVDKAKANPAMFADTGIPLLFKSCYRFGAEKHRMVVKVAGGASILDDT
                     NFFRIGQKNLTALRRIFWKNNILIDGEDTGMNHNRTLSLRIADGVVKVRGSNNFTRKL
                     "
     gene            complement(1658..2545)
                     /locus_tag="P5573_06235"
     CDS             complement(1658..2545)
                     /locus_tag="P5573_06235"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase"
                     /protein_id="HRT27692.1"
                     /translation="MDALKGAAGPDILTRETYAFPMDYELQDHEFEKISRLVYDVCGI
                     ALHEGKREMVRTRLIKRLRNRNFRSFSDYYRYVTAPEGTEELIAMIDSISTNLTNFFR
                     EDGHFARLKSVVQSWRASVRGNGHLPQLRVWSAGCSTGEEPYTIAMVLLELIPLGHVD
                     LKILATDISTAVLKKAAEGVYAEDKIKNVPVALRRKYFLKGVKEWEGFYRVRENLRLP
                     IEFARLNLKEPFSFKEPFDVIFCRNVMIYFDRKMQQLVVNRFYDCLKEGGWLFIGHSE
                     SLTGITHRFKYIEPSVYRK"
     gene            complement(2567..3085)
                     /locus_tag="P5573_06240"
     CDS             complement(2567..3085)
                     /locus_tag="P5573_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HRT27693.1"
                     /translation="MMQAVEAGTEMAVMPQEKSREGKYLTFVLGGEEYGLEILKVKEI
                     IGVMNITAVPQMPRYVKGVINLRGKVIPVVDLRLKFGMESLDYNERTCIIVVDIASGG
                     SKVIMGIVVDCVSEVLNIKAEEIEDAPAFGVRLNTDYILGMAKVKGNVKILLDIDKVL
                     TADVVAAEKVAH"
BASE COUNT          726 a          954 c          735 g          780 t
ORIGIN      
        1 agcggattct ctcatcgctg ccctcggtcg agaccacgat caccggtata tccttgagca
       61 agtcgtcggc cttcaggtgg tcgagcatat ccaggccgct cacacccggc atgttgatgt
      121 ctgagatgat cacgtccacc cagttttcca acaggatgtc gagcgcttcc cgcccgttag
      181 ccgcctcata gcaacggccc accttgaacc cggagatcga gatgattttc cggatcacgg
      241 cccgcatcga gcttgagtca tcgacgataa gaacattgaa ggacataaac ctatcccccg
      301 gtacaacccg actccgtggt gagatcaacc atggaaaaca ttgacgatgt cctccgcgag
      361 tgccagttcc tgggcgagct gaatcatgca ctcttcgata tctctctgcc ggagtttgaa
      421 catctgaacc acctctttca ggccccagta cgccaggccg tcggaccctc cgcccacacc
      481 catcatcaga caaacctggt cggccagata gactacccat ggcgcaattc tctctcttgc
      541 agactgatca ggccgatgat gacaggcgat gacctccaca tgtttcggag ggaaattcca
      601 cctttcagca atcttcccgc ccagctctgc gtggtcgatc ccaaagacct ctttttccgc
      661 cgtgaggaac gcgtaccctt ctttttttac caaccgcgct attttctcga aagaccggtc
      721 tatgtattct ccgagaacga cttttcccac gtcgtgaagt agcgccgccg tgaacagctc
      781 gccgtccttc cggctgtaca actttttcga cagtatctgg gacatgagag caacgcctac
      841 ggagtgtttc cagaggtccg accctttgac gccgtatcct ctcccgctcc tgtaaaaact
      901 caccaccccc gacgcgacca ccgcgcggat gatgttctcc tggcccaaga acatcaccgc
      961 atcctgcacc gtgctgacac gatgccgcga accgaaataa gcggcgttac tcatcttgag
     1021 caggtttgcc gtgatggact ggtcgtactc gatcacggaa acgagctcgg atacactgta
     1081 atccgcgtgg gataggatct ctgccaccct ttgcaccgtg ccggggaagg ccggtatctt
     1141 ctggatggaa tggagaagtt cgtcgacaat catcacaatt tcctcgtgaa attgttcgaa
     1201 cccctgacct taaccacgcc gtcggcgatc cgcaagctga gtgtccggtt atgattcatc
     1261 cccgtatctt cgccgtcgat cagtatgttg ttcttccaga aaatccttcg gagggcggta
     1321 aggttcttct gcccgatgcg gaaaaaattt gtatcgtcca ggatactcgc cccaccggcc
     1381 accttgacga ccatacggtg cttttccgct ccgaatcggt agcaggattt gaaaaggagg
     1441 gggataccgg tgtcggcgaa cattgccgga tttgccttgg ccttgtcaac atctaagttg
     1501 gaatcgggaa gcatgaaatg cagcaacccc ccgacacgca cctgagggtc atatacggct
     1561 acacctatgc aagaccccag cgcgtaagtg atcagcacat cttcctgatt gttgctgacc
     1621 ttcaaatccg agattccgac tatacattct gccatgctta tttgcggtaa acgctcggct
     1681 cgatatattt aaaccgatga gtaatacccg tgaggctttc agaatggccg atgaacagcc
     1741 atccgccctc cttcaggcaa tcatagaacc tgttgacgac aagctgttgc attttccggt
     1801 cgaagtaaat catgacgttc ctgcagaaga tgacgtcgaa tggctccttg aacgaaaaag
     1861 gctctttgag attcaaccgc gcaaactcaa tcggaagccg caggttttct ctgaccctgt
     1921 agaaaccttc ccattcctta acacccttga gaaagtattt gcgtctcagg gcgacgggga
     1981 cgtttttaat tttgtcttca gcgtaaaccc cctctgccgc cttcttcagg acggcagtcg
     2041 aaatgtctgt agccagaatc tttagatcta catgccccaa aggtatcaat tccaacagca
     2101 ccatcgcgat agtgtatggc tcctccccgg tggagcaacc cgcactccat actctcaact
     2161 gcggcagatg tccgttaccc cgcacggaag cccgccagga ttgcaccacc gacttcagcc
     2221 tcgcaaaatg cccgtcttcc cggaaaaagt tcgtcaggtt cgtcgagata gagtcgatca
     2281 tagctatcaa ttcttccgtt ccttcgggcg ccgtcacata ccgatagtaa tctgaaaaag
     2341 aacggaagtt gcggttcctg aggcgtttga tcaggcgtgt cctgaccatt tctctcttgc
     2401 cttcatggag cgcaatgccg cacacgtcgt aaacgaggcg actgatcttc tcaaactcgt
     2461 gatcctgaag ttcgtaatcc atcgggaaag cgtatgtctc tctcgttagg atgtcgggtc
     2521 cagcggcgcc tttaagagcg tccaccaagg ggcattaacc gtcttctcaa tgggccacct
     2581 tctcggcggc gacgacatcg gcagtcagca ccttgtcgat gtcgagaaga atcttaacat
     2641 tgcctttgac ctttgccata ccgaggatat agtcagtgtt caatcgtacg ccgaaggccg
     2701 gcgcgtcctc gatttcctca gccttgatat tcaggacctc ggacacacag tctacgacga
     2761 tacccattat gaccttactg cccccactcg cgatgtcgac gacgatgatg catgtcctct
     2821 cgttgtaatc gagggactcc atgccgaact tcaaacgcaa gtcaacgact gggattacct
     2881 ttcccctgag gttgatcact cctttcacgt accgcggcat ctggggaacc gcagtgatgt
     2941 tcatgacccc gatgatttcc ttgaccttga gtatttcaag gccatactct tctcccccca
     3001 ggacgaaggt cagatacttc ccttctctgc ttttctcctg aggcatcaca gccatttctg
     3061 tccccgcttc caccgcttgc atcatcaata cctcccggtt tttctgtaac gattaatctt
     3121 aatggactcg taaaaagtct cgaattacgc gtttcgtcat tccggcgaac gccggaatcc
     3181 agtatattca atatg
//
LOCUS       DAOTMX010000197         3180 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_23923_length_3180_cov_3.682560, whole genome shotgun sequence.
ACCESSION   DAOTMX010000197 DAOTMX010000000
VERSION     DAOTMX010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23923_length_3180_cov_3.682560"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..928)
                     /locus_tag="P5573_06245"
     CDS             complement(50..928)
                     /locus_tag="P5573_06245"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRT27694.1"
                     /translation="MEFFLQLLVNGVCIGLIYGISAMGFVMIFKASSVINFAHGELLA
                     AGAFFFFTLTAWAGVPVYAAFLLTLAASFILGFIVERLFLRPLIGEPLIEVIMLTIGL
                     SSLFKGLMLLLWGGNLYTFPDFLPESLSLRFGAITIPPVYTGALVIGIFFLALFGLFF
                     KYSSQGIFMRSVADNQTAALSLGVHVKRVFALSWGIAALVAAISGITLGIMNGINVHE
                     LSSIGLKVFPVVILGGLDSITGAVIGGILIGLLETFTGGYLSTSLKDVVPFIVLVIVL
                     LIKPYGLFGLVEIERV"
     gene            complement(971..2911)
                     /locus_tag="P5573_06250"
     CDS             complement(971..2911)
                     /locus_tag="P5573_06250"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRT27695.1"
                     /translation="MSDGVKVPLGLDRKEIAKLTLPQVLRMQAERIGKKGVALREKAY
                     GIWQPYTWHDYAEYVKNTALGLIAVGLRRGDRIGIITNNHVEWLFSELGGQAAGAVTL
                     NLFTTAVAEELVHSLNRVSATYVIVQDQEQADKIIDSREKLSHVRRVIYIDPTGMRPY
                     RSDPWLLSFRELLEIGRAYDSENGGRFVEEVNKGRSKEVAVMIQTSGTTGIPKIAMLT
                     HRNFTAIAEKWLESEPLPVGARWISITPPAWIVDQMWGVGVALLGGLTMNFPETPETV
                     AEDFREIGPDVIITSSRFWEDLASKIRVKMADAGRIKRMFFTAASRIGGEVVEMETRR
                     KPIPSYLRFLNRLASVMVFRPLLDRVGCSMFKSAFTGGHPISPEVIGFFRAAGLNLKQ
                     CYGLTEAGGIFQVQPDGEVRLETVGKPLPGVEVRLSEEQEVLVRSESNFAGYYDDYRS
                     TSESFLDGWLRTGDAGYIDADGHLLIIGRKEEIIRNKEGEAFSPDFIETRLKFSPYIK
                     EAVVFGEGRPFITALLNIDMGNVGNWAEERMIPYTTYSDLSQQEAVQELIRAEVEKVN
                     RQLPDFMKVKKFILLYKLLDADDDELTRTGKVRRRFVYGLYIKLIEAMYGKDREVDVK
                     SRIRYRDGQVGSIDARIRIIEM"
     gene            complement(2904..>3180)
                     /locus_tag="P5573_06255"
     CDS             complement(2904..>3180)
                     /locus_tag="P5573_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRT27696.1"
                     /translation="VALEPSLLVLDEPFAGMNMEEKEDMTRFLIEMNEGWGQTMILVE
                     HDMSIVMSISQRVVVLNFGEKLAEGSPEEIQNHPEVIKAYLGEDVDV"
BASE COUNT          715 a          964 c          818 g          683 t
ORIGIN      
        1 accgttcctt gtaattcccg cagggatgaa aggtcagcct tctcatctct cagacccttt
       61 cgatttctac aaggccgaaa agaccgtagg gcttgatcag caggacgatg acgagcacga
      121 taaagggaac cacatccttg agtgacgtgg acagataacc tccggtgaac gtctccaaaa
      181 gaccgatgag aatcccgcct atcacggcac cggttatcga atcgagcccc cccagtatca
      241 cgaccggaaa gaccttgagc ccgatcgaac tcaactcgtg gacgttgatc ccgttcatga
      301 tgcccaatgt gattccgctg atcgccgcaa ccaacgcggc gataccccac gaaagggcaa
      361 agacacgttt aacatgaacg cccagggaca atgcggctgt ttggttgtcc gccacggacc
      421 tcatgaagat accctgcgaa gaatacttga aaaaaaggcc gaaaagcgcg aggaaaaata
      481 tcccgataac aagggcgccc gtatagacgg gagggatcgt tatggctccg aaccggaggg
      541 ataggctctc cggcagaaag tcggggaagg tatagagatt accaccccag agaagaagca
      601 tcagcccctt gaacaaagac gaaaggccga tcgtcaacat gatgacctct atcagaggtt
      661 cgccgatgag cggccggaga aacaaccgct ccacgatgaa gcccaggatg aaagacgccg
      721 ccagcgtcag aagaaaagcc gcataaacgg gcacgcccgc ccaggccgtg agcgtaaaga
      781 agaagaaggc gccggcagcc aggagctcac cgtgggcgaa gttgatcacg ctcgacgcct
      841 tgaatatcat cacgaaaccc atcgcggaga tgccgtagat gagcccgata cagacgccgt
      901 tcaccagcaa ttgcagaaaa aactccattc agtccctccc gttactccta agtctctgaa
      961 cacctaccgg ctacatctcg atgatccgga tacgtgcatc tatgctgccg acctggccgt
     1021 cgcgatagcg aatcctactc ttgacgtcga cttccctgtc ttttccgtac atcgcttcta
     1081 tgagttttat gtaaaggccg tagacgaagc gcctgcgcac cttgcccgtc ctcgtcagtt
     1141 catcgtcgtc cgcgtccagg agcttgtaga gaaggatgaa cttcttgacc ttcatgaagt
     1201 ccggaagctg cctgtttact ttttccactt ccgccctgat caactcctga acggcttcct
     1261 gctgcgacag gtcgctgtaa gtcgtgtaag gaatcatgcg ctcttccgcc cagttgccga
     1321 cgtttcccat gtcgatgttg agaagcgccg tgataaaagg cctcccttcg ccgaagacca
     1381 ccgcctcctt gatgtagggg ctgaatttca gccttgtctc gatgaagtca ggcgagaagg
     1441 cctctccctc tttgttgcgt atgatctcct cttttcggcc gatgatcaga agatgaccgt
     1501 cggcgtctat gtatccggcg tccccggtcc tcaaccagcc gtcgaggaac gattccgaag
     1561 tcgacctgta gtcgtcgtaa tatcccgcga aattcgattc gctcctcaca aggacctcct
     1621 gctcctcgga gagccgcacc tcaacccccg ggagaggttt gccgaccgtt tcgagacgga
     1681 cctcgccgtc cggctgcacc tggaatatcc caccggcttc cgtcaagccg taacattgct
     1741 tcaggttaag gcccgccgcc cggaagaaac ctatcacctc gggactgatg ggatggcctc
     1801 ccgtgaacgc gcttttaaac atcgagcacc cgaccctgtc gagcagaggc cggaagacca
     1861 tgacggaggc cagcctgttc aagaaacgca aataggacgg aatgggtttc cttcgcgtct
     1921 ccatctcgac tacctcgccg ccgattcggg aagcggcggt aaaaaacatc cgctttatcc
     1981 ggccggcatc agccatcttc acccgtatct tcgacgccag gtcttcccag aacctcgacg
     2041 acgtgattat gacatccggg ccgatctccc tgaaatcctc ggccaccgtt tccggagttt
     2101 cgggaaagtt catagtcaga cctccgagaa gggcgacgcc gacaccccac atctggtcca
     2161 cgatccatgc aggtggggtt atggatatcc atctcgcgcc taccggaagc ggttcgctct
     2221 cgagccattt ttcggctatg gccgtaaagt tcctgtgggt gagcatggct atcttcggaa
     2281 tgccggtcgt tcccgatgtc tggatcatga ccgccacttc tttcgagcga cccttgttga
     2341 cttcttcgac gaaacggcct ccgttttccg agtcgtaggc gcgaccgatc tcaaggagct
     2401 cgcggaaact caggagccac gggtcgctcc tgtagggccg catccctgtg gggtcgatgt
     2461 agatgacccg acggacatgg gacaacttct cccgggaatc aatgatcttg tcggcctgct
     2521 cttggtcctg gacgatcaca taggtcgcac tgacgcggtt cagggagtgg accagttctt
     2581 ccgcgacagc cgtagtgaaa aggttgagcg ttactgcccc ggccgcttga ccgccgagct
     2641 cgctgaacag ccactccacg tggttgttcg ttatgatgcc gatccggtca ccgcgtctca
     2701 gaccgaccgc gatcaaccca agggcggtat tctttacgta ttcggcgtag tcatgccagg
     2761 tatacggttg ccatatcccg taagcctttt cgcggagggc aacgcctttc ttccctatgc
     2821 gctcagcctg catacggagc acctgcggca aggtcagctt tgcgatctct ttcctgtcaa
     2881 ggccgagcgg taccttcacc ccgtcagaca tcgacatctt ctcctagata cgccttgata
     2941 acctcggggt ggttttgaat ttcctcaggc gagccttccg cgagcttttc gccgaaattc
     3001 aagacgacca cccgttgaga tatgctcata acgatcgaca tatcgtgctc tacgaggatc
     3061 atcgtctggc cccatccctc gttcatttcg atgagaaacc gggtcatatc ctccttttcc
     3121 tccatgttca tcccggcaaa gggttcgtca agaaccagga ggcttggctc cagagccacc
//
LOCUS       DAOTMX010000198         3170 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_24005_length_3170_cov_4.239165, whole genome shotgun sequence.
ACCESSION   DAOTMX010000198 DAOTMX010000000
VERSION     DAOTMX010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24005_length_3170_cov_4.239165"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="P5573_06260"
     CDS             complement(<1..495)
                     /locus_tag="P5573_06260"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRT27697.1"
                     /translation="MHGTAETSRILVADDDAVTRQVIVDALKYVKDCSALEASNGEEA
                     LCLLRKHPCNMVITDVRMPVMGGLELLDRIMEINPLTHVIVMTGYPSIDLSVCAMKSG
                     AVDFLVKPFAVDELLHRVDLFLREKSLLEESGAGDRSKVVLRHIINELSTQSYIYDII
                     EETCN"
     gene            complement(547..771)
                     /locus_tag="P5573_06265"
     CDS             complement(547..771)
                     /locus_tag="P5573_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27698.1"
                     /translation="MNISSYQVENILKAYSRHDRVKVQKTAVKEAANDEIYADTVNLS
                     SGKDKASIFNKISYSLTDILLKGGGGTSEE"
     gene            977..2353
                     /locus_tag="P5573_06270"
     CDS             977..2353
                     /locus_tag="P5573_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRT27699.1"
                     /translation="MKKPSILIADDNNVSRDNLFVLLSENGFNVRAVEDGQQAIDAFL
                     GEKFDLVITDLKMPRVDGLQLLEFIKGVNPESVVVMVTGHGTVGTAVEAMKLGAFDYV
                     TKPLKDDLVMLTVERALSFAKLREENATLKSHLKKQYGFGKMIGYSDAMKRVFETIEK
                     VASSDSTVIIYGESGTGKELVAKAIHFNSDRSGFPLVAVNCCAIPEDLLESELFGHEK
                     GAFTGATQSRVGRFELAAGGTIFLDEIAEMSPALQVKLLRVIQEREFERIGGVRTISV
                     DVRIIAATNQDLEKAVKERRFREDLYYRINVIPIHLPPLRGRKADISILANHFLSKFN
                     KAKKKNIGRILPEAMAYLERYPWPGNVRELENLIEMLVVMKETGDIAAADLPAKILNA
                     RGKENAAPPESIPDEGVDFNSLVTGFEKDLLLRALSKTGGAKKSAAKLLNLNRTTFVE
                     KLKRLNIS"
     gene            2679..>3170
                     /locus_tag="P5573_06275"
     CDS             2679..>3170
                     /locus_tag="P5573_06275"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRT27700.1"
                     /translation="MAAVLLLGFLGGAELYAAASKDTAYRLQIACYRDEGQAIGLTRS
                     MRKLGYSSFYEKAAIEGKGEWYRVYLGRFKTRAETQAAGKKLVRDGVIDNFLVREPNF
                     AKARESGAHAGMTKTKLRPARQDSRDAPRETKTETSKDKAAGEEDSPPKVSQDAAAAA
                     QPVP"
BASE COUNT          752 a          800 c          913 g          705 t
ORIGIN      
        1 gttgcaggtc tcttcgatga tgtcgtagat atagctttga gtggacaatt catttattat
       61 gtgccgcaaa acgaccttcg acctatcgcc ggcgccgctt tcttcgagga gggacttttc
      121 cctcaggaaa aggtcgaccc tgtgaagcag ctcgtcgacc gcgaaaggtt tgaccagaaa
      181 gtcgacggcc ccgcttttca tggcacagac cgaaaggtcg atagacggat aaccggtcat
      241 cacgatgacg tgggtcagcg ggtttatctc catgatacgg tcgagaagct cgaggccccc
      301 catcacgggc atcctgacgt cggtgatgac catgttgcaa gggtgtttcc tgagaagaca
      361 cagtgcctct tcgccgttcg acgcttccaa tgccgagcaa tcctttacgt acttcaaggc
      421 atcgacaatc acttgtcggg tgactgcgtc gtcatcggcc acgaggattc tgctggtttc
      481 agcggtaccg tgcatggtga aaattgtcct cggaaggttt tggatgtctc gatgaattcg
      541 aaggcgctac tcttcggatg tccctccgcc gcccttgaga aggatatccg tcaagctgta
      601 cgaaatcttg ttgaaaatcg atgccttgtc cttccccgaa gaaaggttaa ccgtatccgc
      661 gtatatttcg tcgttcgccg cctccttgac cgcggtcttc tggaccttga cccggtcatg
      721 tctgctgtaa gccttcagga tgttttcgac ctggtatgaa gagatgttca tggcaccgtc
      781 tcgtcgcttc tattttcgtt acactgctta tcggacaatg tatcttaaaa ctttagaaaa
      841 atttggggat aaattgtgtt tgactatcgg gatgtttttt tctatatatc cacaggtagt
      901 taacgtcggc cgtcgcaggc cgacgtcatc ttttcccgtc ctttaaattt tatgtgaccg
      961 aatctcctga aatttgatga aaaaacccag catattgatc gctgacgaca acaatgtcag
     1021 cagggacaat ctgtttgtgc tgctgtcgga aaacggcttc aacgtcaggg ccgtcgaaga
     1081 cggccagcag gcgatcgacg ccttccttgg ggaaaaattc gacctcgtga tcacggactt
     1141 gaagatgccc cgggtggacg gcctgcagtt gctcgagttc atcaaggggg tgaacccgga
     1201 gagtgtcgtc gttatggtaa ccggccatgg gacggtcggc acggccgtcg aggcgatgaa
     1261 actcggcgcc tttgattacg tgacgaagcc cttgaaggac gacctcgtca tgctgaccgt
     1321 cgagcgggcg ctgtcgtttg cgaaactgag ggaagagaac gcgacactga aaagtcatct
     1381 gaaaaagcag tacggtttcg ggaagatgat cgggtacagc gacgcgatga agagggtgtt
     1441 cgagacgatc gaaaaggtcg cttcttccga cagcacagtc ataatctacg gggaaagcgg
     1501 cacgggcaag gaactcgtag cgaaggccat ccacttcaac agcgaccgca gcggcttccc
     1561 gctggtcgcc gtcaactgct gtgcgatacc cgaagacttg ttggaaagcg agttgttcgg
     1621 gcatgaaaaa ggtgccttta cgggggcgac tcagagcagg gtcggtagat tcgaacttgc
     1681 cgctggcggg accatctttc tcgacgagat cgccgaaatg agcccggcac tccaggtaaa
     1741 actgttgcgg gtgatccagg aaagggagtt cgaacgtatc gggggggtga ggactatctc
     1801 cgtcgatgtg cggatcatcg ccgccaccaa tcaagacctg gaaaaggcgg tgaaagaaag
     1861 gcgtttccgt gaggacttgt actaccgcat caacgtgatt cccatccacc tgcctcctct
     1921 gagggggagg aaagcggata tatcgattct ggcgaatcac tttctgtcta agttcaacaa
     1981 agcgaaaaaa aagaatatcg gcaggatact gcccgaggcc atggcatacc tggaaaggta
     2041 cccctggccg ggtaacgtga gagagctcga gaacctcatc gagatgctgg ttgtgatgaa
     2101 agaaacaggc gatatagccg ctgccgacct cccggcgaag atcttgaacg cccgggggaa
     2161 agaaaacgcc gcccccccgg agagcatccc cgacgagggc gtcgatttca actccctcgt
     2221 gaccgggttt gagaaggacc tgctgctgcg ggccctgtcg aaaacgggag gggcgaagaa
     2281 aagcgccgcg aaactcttga acctcaaccg aactaccttc gtcgagaagc taaaaagact
     2341 caacatctcc tgacgttaca cacctaccct ttattcgttt cattttcccc ccaacgcccg
     2401 gcattaaact cctgcctcat tgtcaaatta ttgtctctta gaatgatggg gaattgacaa
     2461 gattgcccct tgagacatcg gcccggcgaa aaatcccgtc ttttcgcgtg atccggcggg
     2521 taacgactgt cgaaaggctg gtatgcaaat tgctgaaagt cttcttgaaa ccgggggggc
     2581 agggttgggg gcctttaccc gtcggtttca aataagtcgt atcttctgcg ggttacgaaa
     2641 tgcttcggtc tttgcacgtc ggaaaaggca tagtatgtgt ggccgccgtt ctgctgctgg
     2701 gattcttggg cggtgccgaa ctctacgcgg cagcctcgaa agataccgct tatcgactcc
     2761 agatcgcctg ttaccgggat gaaggacagg cgatcgggct tactcggtcg atgagaaaac
     2821 tcggttactc gtcgttctat gaaaaagcgg ctatcgaggg taagggggag tggtacaggg
     2881 tttatctggg tcggttcaag accagggcgg agactcaagc ggccgggaaa aaacttgtgc
     2941 gggacggtgt tatcgataac tttctggtac gggagcctaa ctttgccaag gcaagggaat
     3001 caggagcgca cgccgggatg acgaaaacaa agcttaggcc ggccaggcag gacagccgag
     3061 acgctccgcg ggagacgaag acagagacaa gtaaggataa ggcggcgggg gaagaggact
     3121 cgccgccaaa ggtctctcag gacgcggccg cagccgctca accggttccg
//
LOCUS       DAOTMX010000199         3166 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_24038_length_3166_cov_3.649309, whole genome shotgun sequence.
ACCESSION   DAOTMX010000199 DAOTMX010000000
VERSION     DAOTMX010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24038_length_3166_cov_3.649309"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..945)
                     /gene="rfaE1"
                     /locus_tag="P5573_06280"
     CDS             complement(<1..945)
                     /gene="rfaE1"
                     /locus_tag="P5573_06280"
                     /inference="COORDINATES: protein motif:HMM:TIGR02198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HRT27701.1"
                     /translation="MMKILNARKARQIVDDFSHATVLVVGDVMVDHFIWGKVSRISPE
                     APVPVVEVGRESHLLGGSANVMNNVIALGGKVFVCGVIGDDGMGRWLLDELGRLKVDT
                     DGIVVEDDRPTTIKTRIVAHSQQVVRFDRESRQPVRQASVERVLEYIQKKRDELGAVV
                     ISDYNKGMVSGPLLDGIRKIMQGSGVVTCVDPKHSDFSLYRGFDVITPNHHEAESALG
                     VEDINGEFALKEAEVSRAAEHLMDRLQLKAILITRGEEGMSLYERNGGITHIPTVARE
                     VFDVTGAGDTAIGVFALSIAAGASFREAAVLANQAAGIA"
     gene            complement(942..1922)
                     /gene="galE"
                     /locus_tag="P5573_06285"
     CDS             complement(942..1922)
                     /gene="galE"
                     /locus_tag="P5573_06285"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HRT27702.1"
                     /translation="MILVTGGAGYIGSHTVKVLHERGYDTVTFDNLSRGHREAVKWGK
                     FIEGDLSDTGLLAEIFRNYGVKAVLHFAAHSLVGESEEDPRRYYMNNVVNGIRMLDAM
                     LAAGVRKIVFSSSAAVYGEPLRLPIDEGHPVEPVNVYGQTKLIMENILSKYAPTYGLE
                     YLSLRYFNAAGADPDGLIGEDHDPETHLIPLILDAALGRREEVTIFGTDYDTPDGTCV
                     RDYIHVTDLAEAHILALEHLERGGKSGIYNLGNGKGCSVREVIRAAERVTGKKIRARD
                     SGRRRGDPARLVASSERISRDLGWRPRFGDLDVIVETAWEWHRRRFGGRS"
     gene            complement(1919..2911)
                     /locus_tag="P5573_06290"
     CDS             complement(1919..2911)
                     /locus_tag="P5573_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRT27703.1"
                     /translation="MKENDRTSRGNSTRHLPPEVERTLITGGAGFLGSHLCDFFLEKG
                     HAVICMDNLITGNIDNVAHLFGNERFSFIKHDVTEYIYVEGRVDNILHFASPASPIDY
                     LRLPIQTLKVGSLGTHKALGLAKAKGAKFLLASTSECYGDPLVHPQSEDYWGNVNPIG
                     PRGVYDEAKRFAEAMTMAYHRYHGLDTRIARIFNTYGTRMRLDDGRALPTFMRQALRG
                     EDLTVFGDGRQTRSFCYVDDLVEGLWRLLNSGEVEPVNLGNPQEMTILLLAEKVSGFC
                     GGGKKIVFKDLPVDDPKVRQPDISKARRVLGWEPKVPLDEGLRRVFAYFNERAS"
BASE COUNT          616 a          972 c          861 g          717 t
ORIGIN      
        1 cgcgatcccc gccgcctggt tggccagcac tgctgcctcc ctgaacgaag ctcccgcggc
       61 gatggaaagg gcaaaaacgc ctatcgccgt gtctcctgcc ccggtcacgt cgaacacttc
      121 tctggcaacg gtggggatgt gggtgatacc accgtttctc tcgtacagac tcatcccctc
      181 ctcaccccgg gtgatgagga tcgctttcaa ctggagtcgg tccatgaggt gttcggcggc
      241 ccggctgacc tcggcttcct tcaaggcgaa ttcaccgttg atgtcttcga ccccgagggc
      301 cgactcggcc tcgtggtgat tgggcgtgat cacatcgaac ccgcgataga gagagaagtc
      361 gctgtgcttc gggtcgacac aggtcactac gccgcttccc tgcatgattt tccggatacc
      421 gtcgaggagc gggccggata ccattccctt gttgtagtcc gatatcacaa cggcccccag
      481 ttcgtcgcgt ttcttttgta tgtactcgag gactctctct acactcgcct gccggacggg
      541 ctgtctgctc tcccggtcga accgaaccac ctgctgactg tgggcgacga tccttgtctt
      601 tatcgtcgtg ggccggtcgt cctcgacgac gataccgtcg gtgtccacct tcaaccttcc
      661 cagttcatcc aaaagccagc gtcccatccc gtcgtcgccg atcacgccgc agacaaagac
      721 cttcccgccc agggcgatga cattgttcat gacattcgcg gaacccccca gcaggtggct
      781 ttcccgtccg acctccacga cgggcacggg cgcctcgggc gatatccggg agaccttgcc
      841 ccatatgaag tggtcaacca tgacatcgcc gacgaccaag accgtggcgt gcgaaaaatc
      901 gtccacgatc tgcctcgcct tcctggcgtt caggatcttc atcatgatct tcccccgaac
      961 ctcctccgat gccattccca cgccgtctcg acgataacgt ctaggtcacc gaaacggggc
     1021 ctccatccca ggtccctcga tatcctctcg gaacttgcga caagcctcgc cgggtcgccc
     1081 cgcctccggc cgctgtctct cgccctgatt ttcttccccg tcacccgctc ggcagccctg
     1141 atgacctcgc gaaccgagca accctttccg tttccgaggt tgtagatacc ggactttccg
     1201 cctctttcga ggtgttccaa ggccaggatg tgtgcctctg ccaggtccgt cacgtggatg
     1261 taatcccgga cgcaggtgcc gtcgggtgtg tcgtagtcgg tgccgaagat cgtcacctct
     1321 tctcgtctac ccagggcagc gtcaagtatc aacgggataa ggtgggtttc cgggtcatgg
     1381 tcctccccaa taaggccgtc cgggtcggcc cccgccgcat tgaagtaccg gagggataag
     1441 tactcgaggc cgtaagtagg cgcatatttc gacaggatgt tctccatgat cagtttcgtc
     1501 tgaccgtata cgttgaccgg ttccacagga tggccttcgt ctatgggcaa ccgcaacggt
     1561 tctccgtaga ccgcggcgct cgaggagaag acgatctttc ggacccccgc agcgagcatg
     1621 gcgtcgagca tacggatgcc gttgacgacg ttattcatgt aataacggcg ggggtcttct
     1681 tccgactcgc ccacgagact atgggcggca aaatgaagga ccgccttgac gccgtaattc
     1741 ctgaaaattt cggcgaggag acccgtatcc gagaggtccc cctcgatgaa ctttccccac
     1801 ttaaccgctt cccggtggcc cctggagagg ttgtcgaagg tgacggtgtc gtaaccccgt
     1861 tcgtggagca ccttcaccgt gtgggaaccg atgtatcccg caccgccggt aacaagtatc
     1921 atgaagccct ttcgttgaag taggcgaaca ccctcctgag accctcgtcc aatggcacct
     1981 taggctccca cccgaggacg cgtcttgcct tagagatgtc gggttgtctt actttcgggt
     2041 cgtccacggg gaggtccttg aagacgatct tcttcccgcc cccgcagaac ccggagacct
     2101 tttccgcaag caaaaggata gtcatttcct gggggttgcc caggttgacg ggttcgactt
     2161 caccggagtt caggaggcgc cagaggccct ccaccaggtc atcgacgtag cagaaactgc
     2221 gggtctgccg tccgtctccg aaaacggtca ggtcctctcc cctgagggcc tgcctcatga
     2281 aggtcgggag ggcgcggccg tcgtcgagcc gcatccttgt accataagta ttgaatatcc
     2341 tggcaatcct cgtatcgagg ccatggtaac ggtgataggc catggtcatt gcttccgcga
     2401 agcgctttgc ttcgtcgtag accccccgcg gcccgatggg gttgacgttt ccccagtaat
     2461 cctcggactg ggggtgcaca agcgggtctc cgtaacactc cgacgtggag gcaaggagaa
     2521 acttcgcccc ttttgccttc gccaggccca aggccttgtg ggttccgaga gagccaacct
     2581 tcagcgtctg gatcggcagc ctgaggtagt cgatcgggct tgccggcgat gcgaaatgaa
     2641 ggatgttgtc gaccctgccc tccacgtaga tatactcggt aacgtcatgc ttgataaaag
     2701 agaacctttc gtttccgaag agatgggcga cgttgtcgat gttgcccgtg atcaggttgt
     2761 ccatgcagat gacggcgtgc cccttttcga ggaaaaaatc gcagaggtgg cttccgagga
     2821 aaccagcccc tccggtaatc agtgtccgct cgacttcggg cggaagatgc cgggtgctat
     2881 ttcctcggga tgttctatcg ttttccttca tgtccggtaa tctgacctat gctgaaagcc
     2941 gggctcagac cttcctttgg cactctatgt ctctgaaaca ggataacgaa tgctattatc
     3001 agggcctttc tttgtcaacg actttctccc taaaaaacaa gttctttctt gacttaatcc
     3061 taaaaacgat atagccatat taactttact taaagctgta aggtgccaag gtgctgatga
     3121 aagaaaagga aagcgacttt ctcaggtcac gacgggaaaa gataga
//
LOCUS       DAOTMX010000200         3163 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_24073_length_3163_cov_3.331403, whole genome shotgun sequence.
ACCESSION   DAOTMX010000200 DAOTMX010000000
VERSION     DAOTMX010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24073_length_3163_cov_3.331403"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="P5573_06295"
     CDS             <1..753
                     /locus_tag="P5573_06295"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRT27704.1"
                     /translation="EYLALIGPNGCGKTTLIQHLNALILPERGKVIVDGMATDDPRNR
                     REIRRRVGMVFQNPDNQIVGTTVEEDIAFGPGNLGLPPHEIKRRVEDALEATGIRNLA
                     ERDPNSLSGGEKRLVAVAGVLAMKPRYIALDEPTSYLDPAGKRRLLAVIKKLNEEGLT
                     VIHVTHNMNDVVQADKVVVMLEGRIFKAGTPKMIFSDPETLVRSGLGIPGALEILQGL
                     RGRGWNVRTDLLTTEEACEEIAACLKREGVTR"
     gene            772..1557
                     /locus_tag="P5573_06300"
     CDS             772..1557
                     /locus_tag="P5573_06300"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HRT27705.1"
                     /translation="MYHFGQYVCSDSPVHRLDPRVKVLSVVALSIITLKAGLYSALLI
                     SAFIVLTGTTARLTVRRFMDSLKPLVPFLAVLFALHLLLTDGAPLLNVLPGVAVTSEG
                     LRRGILTTWQFAALVAVASILTLTTSPSELISGIERLLRPFGKIGVPSHDIAVMISLA
                     LRFVPTLLAEAEKMKKAQLSRGADFSQGSIIRRAKAIAALAVPLVLGSFRRAEELARA
                     MEGRGYERGPRTYLRELRLTPSDYRASVLMVVFLAGSHFLNAL"
     gene            1724..>3163
                     /locus_tag="P5573_06305"
     CDS             1724..>3163
                     /locus_tag="P5573_06305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRT27706.1"
                     /translation="MVMPMDFHRFYEAIVRYSPLAIIALDRDGNILLWNPAAEALLGW
                     EEEEVVGRPLTAFFPDERETEEFKRFLAAFEEDKPCSTRRARLKRKDGTAVDVDVFTS
                     LLQEEGRSVGVVGIIADITHHKQSEDFYKTIADSSYAGVYVVQKGKFVFINRKAAALA
                     GYTPEEMTGMESFSIVHPEDRGFARLSASLMLKGKRSAPYAFRVITKDGQVRWIMETV
                     TSILYDGERAILGNSMDITERKRIEEALRQSEERYRTILENIDDGYYETDLEGNFILF
                     NEAFEVILGYGKDELTGMNYRKVLDGETAPVVSKTFARVCRTGKPERGEWSVTKKDGS
                     RGFLEVSVALIRNAAGEPTGFRGIIHDITARKEAERAITHLAYHDPLTGLPNRILFND
                     RLSMAIIHSQRKKLKFALLILDLDNFKQVNDTFGHSMGDRLLCSVGKRLLNLLRKSDT
                     VARMGGDEFLILLQDIARPESAVDSASASL"
BASE COUNT          696 a          951 c          927 g          589 t
ORIGIN      
        1 gagtacctgg ccttgatagg gccgaacggc tgcggaaaaa cgaccttgat ccagcacctc
       61 aacgccctga tcctgccgga aaggggaaag gtcatcgtcg acgggatggc taccgacgac
      121 ccgcggaacc ggcgggagat aagacgacgg gtgggcatgg tcttccagaa ccccgacaac
      181 cagatcgtgg gaacgacggt ggaagaagac atcgccttcg gcccgggaaa tctggggctt
      241 ccgccgcacg agatcaagag gcgtgtcgag gatgcccttg aggccaccgg tatccgaaac
      301 ctggccgaac gcgaccccaa cagtctctcc ggcggggaga agagactcgt tgccgtcgcc
      361 ggtgtactgg ccatgaaacc ccggtacatc gccctcgacg aacccacatc ctacctggac
      421 ccggcaggga aaaggcggct cctcgccgtc atcaagaaac tgaacgagga aggactcact
      481 gtcatccacg taacccacaa catgaacgac gtcgtgcagg cggacaaagt cgtggtgatg
      541 ctggaaggtc gtatcttcaa ggcgggcacg ccgaagatga tcttttccga cccggagacg
      601 ctggtccgat cagggctcgg catcccgggg gcgctggaga tactgcaagg gctgcgcggg
      661 cggggctgga acgtccggac cgacctcctg acgacggaag aggcctgcga ggaaatcgcg
      721 gcctgcctca agagggaagg cgtcacccga taacggcata agtgcgagac catgtatcac
      781 ttcggacaat acgtatgcag cgactctccg gtccaccgcc tggacccacg ggtaaaggtg
      841 ctttcggtcg tcgccttgag catcataacc ctgaaggccg gcctttacag cgcgctcttg
      901 ataagcgcct tcatcgtcct gacgggcacg accgcccggt tgacggtgcg ccgtttcatg
      961 gactccctca agccccttgt gccctttctc gcggtgctct tcgccctcca tctcctcctc
     1021 acagacggtg ccccccttct gaacgtactg ccgggggtcg ccgtcacttc agaggggctc
     1081 cgcaggggca tcctcaccac ctggcagttt gcggcactgg tggcggtggc gtccatcctg
     1141 accctcacta cctcgccctc ggaactgata agcggcatag aacggcttct ccgtcctttc
     1201 ggaaaaatcg gtgtcccttc acacgacatc gcggtcatga tctccctcgc cctacggttt
     1261 gtgccgaccc tcctcgccga ggcggaaaag atgaaaaagg cacaactctc caggggggcc
     1321 gatttcagtc aagggagcat catccggcgt gccaaggcaa tcgcggccct ggccgtgccc
     1381 ctggttctgg ggtccttccg gcgggccgag gaactggcaa gggcgatgga aggccgtggg
     1441 tacgagcggg ggccacggac gtacttgaga gaacttcgcc taacgccatc ggactaccgg
     1501 gcatcagtcc tcatggtcgt gttcttggcg gggagccatt tcctcaacgc cctttagaga
     1561 tccccgaaag gcctctcccg caccaccgtt tcgagaagac caccgttgct ttttccccat
     1621 attattgtat agaatttaac gaaaaggggg gctgtggtaa ggtccctttt tcggttttca
     1681 ctgtcgactc tactaaaaaa ttttgcgcaa aggtccggat tccatggtca tgcccatgga
     1741 ctttcatcga ttctacgagg cgattgtccg ctactcaccc ttggcgatca tcgccttgga
     1801 tagggatggg aacatactcc tgtggaatcc ggcagcggag gccctcctcg gttgggaaga
     1861 agaggaggtc gtgggaaggc ctctcaccgc ctttttcccc gacgagcgtg aaacggaaga
     1921 attcaagcga tttttggctg cctttgagga ggacaagcct tgctcgaccc gacgggcccg
     1981 cctcaaaaga aaagacggga cggccgtcga tgtcgatgtc tttacgtccc tgcttcagga
     2041 ggaaggcaga agcgtcggcg tcgtcggcat aatcgccgac ataacccacc acaaacaatc
     2101 ggaagacttc tacaagacga tagccgacag ttcctatgct ggggtctacg tcgtccagaa
     2161 gggcaaattc gtgttcatca accgcaaagc cgccgccctc gcgggctata cccccgagga
     2221 gatgacgggg atggagtcct tcagtatcgt acaccccgag gatagaggct tcgcacggct
     2281 cagcgcatcg ttgatgctga aggggaaacg ctctgccccg tatgccttcc gggtcatcac
     2341 caaggacggg caggtccggt ggatcatgga aacggtgacg tcgattctgt atgacggaga
     2401 gagggcgatc ctgggaaact cgatggacat taccgagcga aagcggatag aggaggcctt
     2461 gcggcagagc gaagagcggt acaggaccat cctcgaaaac atcgatgacg gctactacga
     2521 gaccgatctc gagggaaatt tcatcctctt caacgaggcc ttcgaggtga tactcgggta
     2581 cggcaaggac gaactgaccg ggatgaacta ccgcaaagtg ctggacggag agaccgcacc
     2641 ggttgtatct aaaacttttg cccgcgtctg ccggacgggc aaaccggaaa ggggagaatg
     2701 gagtgtcacc aagaaagacg gctccagggg tttcctggag gtctctgtcg ccctgatccg
     2761 aaacgccgcg ggagagccga caggattccg cggcatcatc cacgacatca ccgcacggaa
     2821 ggaagcggaa cgggcgatca cccacctggc ctaccacgac cccctcacgg gactgcccaa
     2881 tcgcatcctc ttcaacgacc gcctctccat ggcgatcatc cattctcagc gcaagaaact
     2941 gaagttcgcc ctgctgatcc tcgacctcga caatttcaaa caggtcaacg acaccttcgg
     3001 ccactctatg ggtgaccgcc tcctgtgcag tgtagggaag cgcttgctga atctcctgcg
     3061 caagagcgac acggtagccc gcatgggggg tgatgagttc ctcatcctcc tgcaggatat
     3121 cgcccgcccg gaaagcgcag tagattcagc aagcgcttcc cta
//
LOCUS       DAOTMX010000201         3162 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_24084_length_3162_cov_3.683939, whole genome shotgun sequence.
ACCESSION   DAOTMX010000201 DAOTMX010000000
VERSION     DAOTMX010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24084_length_3162_cov_3.683939"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="P5573_06310"
     CDS             <1..766
                     /locus_tag="P5573_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRT27707.1"
                     /translation="AAQAGVRYSLENPYAYIDSNIVGFMNILEGCRYKGVQHLVFASS
                     SSVYGANTLMPFSVHHNVDHPISLYAATKKANELMAHVYSSLYKIPCTGLRFFTVYGP
                     WGRPDMALFSFTKSILEGTYIDVFNYGKMKRDFTYIDDIIEGVARIMDRVPAANARWN
                     GKAPDPGTSFAPYKIYNIGNNSPVGLMEFIGAIEKCLGKKAKKNFLPMQPGDVPKTYA
                     DIDDLMKAVGFRPSTSIGEGIRKFVTWYKKYYKINT"
     gene            869..2782
                     /locus_tag="P5573_06315"
     CDS             869..2782
                     /locus_tag="P5573_06315"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HRT27708.1"
                     /translation="MTRIFGYQNFSGRPSGRPSHIRPDFQPVNENIGDDGAIYWSDNL
                     SKKKSESTYESPDLLVIVMGNVYRKDSLFSRYPELKDTEDQGCAAKIAHLYSLDKHEV
                     LADLTGDFILIIKDEKEKKLILIRDKMGGRKLYFTVLEGYLIFSSHMKSLLNVPGVSR
                     EINRFALESFFKFGYVISPDTIFKSIKELEPGHWIKCGKGSCKVVQYWKLRPSTEIGL
                     TGGSLREALYEKIEENIHARVAADERIGVYLSGGIDSNTLLAVLSRVRDPSKIQTMSI
                     GFGEEYKDYHELNQARLAASHFGSSHHELINGPEHIDRHLCRMVWQFEQPFGNPSLIS
                     LVSLSDLAANLVDVVFVGAGADEVLGGYKRYNALTFLQLYNKIPFSRQSGRLFRSLLS
                     RMPVGADHYSIINRIKKLSRSVQPDILKANEALLFGSYDELRDNLFLAGFLKTNRESE
                     PSLAPFYHAAGSSDPFMQIFVADCYSDLVSEQLTRALVPLAELGIDYRAPFCDPGFME
                     FCLNIPNRYKAGLFRTKIVYKDAVESILPAQIMKQKKRGLSHPVGFWLQGPLYERLTS
                     ILSFDNPALIDYFDMNFLRRICGEHYSKKQNWSELLWKIIVFTMWHKIFIENSFTGMP
                     DWSLKDLGRLGNG"
     gene            complement(2904..2981)
                     /locus_tag="P5573_06320"
     tRNA            complement(2904..2981)
                     /locus_tag="P5573_06320"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
BASE COUNT          824 a          792 c          791 g          755 t
ORIGIN      
        1 tgcagcacaa gctggtgtcc gctactctct cgaaaacccc tacgcctaca tcgacagcaa
       61 catcgtgggc ttcatgaaca tcctcgaggg ctgccgctat aagggcgtac agcacctcgt
      121 cttcgcctcc tcgagttccg tctatggcgc aaacaccctg atgccctttt ccgttcacca
      181 caacgtggac caccccataa gcctctacgc ggctacaaag aaggcaaacg agcttatggc
      241 ccatgtgtat tccagccttt acaaaatccc ctgcacgggc ctcagattct tcaccgtcta
      301 cggaccctgg gggaggcccg acatggcact tttctccttc acgaaatcga tcctggaggg
      361 gacctacatc gacgttttca attacggcaa gatgaagagg gactttacct acatcgacga
      421 tatcatagag ggtgttgcga ggatcatgga cagggtcccc gcggccaacg cgcggtggaa
      481 cggcaaggct ccggacccgg gcacgagttt cgccccctac aagatctaca atatcggcaa
      541 caacagtccc gtgggcctga tggagttcat tggcgccatc gagaaatgcc tcgggaagaa
      601 ggcgaagaag aacttcctcc cgatgcagcc cggcgacgtc cccaaaacct acgccgatat
      661 cgacgacctc atgaaggccg tgggcttcag gccatccacg tccatcggag agggcatccg
      721 aaaattcgtg acgtggtaca aaaaatatta caaaatcaat acctaactta tcaatataac
      781 agggcaatcc agggagggtc gaacccccac ggagttcgat tcacgccgga atggttgcat
      841 ttattttcat cagaatagaa taaagacaat gactcggata ttcgggtacc agaatttcag
      901 cggcaggccg tcaggtcgtc cttcgcatat tcgaccggat ttccagcctg tcaatgaaaa
      961 cataggcgat gatggagcga tatactggag cgataaccta tcaaaaaaga aaagtgaatc
     1021 aacctatgaa agcccggatt tgctggtcat cgtcatggga aatgtctatc gtaaagacag
     1081 cttgttttca cgatatccgg aattgaaaga cacagaggat caaggctgcg cagcgaaaat
     1141 agcgcatcta tacagccttg ataagcatga ggtgcttgca gacttgacgg gcgatttcat
     1201 cctcatcatc aaagatgaaa aggagaaaaa gctcattctc atccgggata aaatgggggg
     1261 gcgaaagctc tactttacgg ttttggaagg gtacttaatc ttttcttccc atatgaagtc
     1321 gttgctgaac gttcccggcg tctctcgcga gatcaaccgt tttgctctcg agagtttctt
     1381 caagttcggg tatgtgatat cgcccgatac catattcaaa tcgataaaag agctggaacc
     1441 cggtcactgg atcaaatgcg gaaagggcag ctgcaaggtt gttcaatatt ggaagcttcg
     1501 tccttccacc gaaataggct tgaccggcgg cagcctccgg gaggcgctgt atgaaaaaat
     1561 cgaggaaaac attcatgcca gggtcgctgc cgacgaacgg attggtgtat atctcagtgg
     1621 cggaatagat tcaaacaccc tgctcgcggt attaagccgt gttcgtgatc catcgaagat
     1681 tcagaccatg agcatcgggt tcggggaaga atacaaggat tatcacgaac tgaatcaggc
     1741 acgcctggcg gcaagtcatt ttggatccag ccatcacgag ttgatcaatg gccccgagca
     1801 tatagacagg catttgtgca gaatggtgtg gcagtttgag cagccctttg gaaatccatc
     1861 gctgatttcc ctggtcagcc tgagtgattt ggccgcgaat ctcgttgatg ttgtctttgt
     1921 cggggcggga gccgatgaag ttctcggcgg ctacaagagg tacaatgctt taacattttt
     1981 acagttatac aataaaatcc ccttttcccg gcagtcaggc aggcttttca ggtcgcttct
     2041 ttcccgcatg cctgtcgggg cagatcatta cagcatcatc aacaggatca aaaagctttc
     2101 ccgatctgtc cagccggata tcctcaaagc caatgaagcc cttctcttcg gaagctatga
     2161 cgaactcaga gataatctct ttcttgcggg tttcctgaag acgaaccgtg aatcagagcc
     2221 ttccctcgcg ccattttacc atgcagccgg cagttctgat ccattcatgc aaattttcgt
     2281 tgccgactgc tattccgatc tcgtcagtga acagttgaca agggctcttg taccgcttgc
     2341 cgagctgggc atcgactaca gggcgccgtt ttgcgacccc gggttcatgg aattttgcct
     2401 caatatcccc aacaggtaca aagccggttt attcagaacc aaaatcgtct acaaagacgc
     2461 ggtagaaagc atcctgccgg cgcagattat gaagcagaaa aagaggggcc tttcacatcc
     2521 tgtcggcttt tggctgcagg ggccgctcta cgagagactc acgtcgattt tgtcttttga
     2581 taatccagcc ctgattgact atttcgatat gaatttcctg cgcagaattt gcggggagca
     2641 ttattccaaa aagcaaaact ggtcggaatt gctgtggaaa ataatcgttt ttacaatgtg
     2701 gcataaaatc tttattgaaa attcttttac tggcatgccg gattggagct taaaggacct
     2761 cggccgttta ggaaatgggt gactgtgcgg gctcaggggc catgagaaaa ggaagggaag
     2821 ctcgataatg attcgccccc gcatgctcga aagaaagcat aaaaaaaaga cagggccggt
     2881 gatggccctg cctttgatga gcatggtgga gctgagagga attgaacctc tacgccatca
     2941 gtattgctac caatgtgctc tcccggttga gctacagccc cggaaacttt ttattctata
     3001 tccagccagg gttaaccttt tcagattcac cctttatgat catggtaggc ggtgctggat
     3061 ttgaaccagc gacttccacc gtgtgagggt agcactctcc cgctgagtta ttaaccagtc
     3121 cataatagaa cggacatcat gacgcgtata gagtggatgg cg
//
LOCUS       DAOTMX010000202         3159 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_24111_length_3159_cov_4.546070, whole genome shotgun sequence.
ACCESSION   DAOTMX010000202 DAOTMX010000000
VERSION     DAOTMX010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24111_length_3159_cov_4.546070"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="P5573_06325"
     CDS             <1..352
                     /locus_tag="P5573_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-glucan phosphorylase"
                     /protein_id="HRT27709.1"
                     /translation="PLEACGTSGMKAALNGVPHLSILDGWWIEAYNGRNGWAFGGEVA
                     GENRDPADAEAIYNLLESQIIPLYYDVAENGTSPGWLKVMKEAIQSNFPRFSARRMVR
                     EYNEKFYTPALRKA"
     gene            complement(364..1137)
                     /locus_tag="P5573_06330"
     CDS             complement(364..1137)
                     /locus_tag="P5573_06330"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRT27710.1"
                     /translation="MFKRIIEGFRRLRQKPDLEKDAGCYWGMSDREARIQDQSHWCGV
                     MRWDSDRWYEHGDLYLRLTLKYLERYAGVDYVAGLNRLVALEWGCGGGAVVRPLCQRF
                     ASVYGVDVSKASVAESVRRMEYLGLLNYRGIAFPSDDPESVLDGGTAGSVDFIVSVNV
                     FQHFPSKPYAARVLKVMGTLLKPGGFALLQTRYFDGSRKLQQKDDDYARNVIYMTSFT
                     PGEFLLLLEAAGLELLERTRDVEGDPDCHDYYFVRKVND"
     gene            complement(1152..2612)
                     /locus_tag="P5573_06335"
     CDS             complement(1152..2612)
                     /locus_tag="P5573_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HRT27711.1"
                     /translation="MTENRSIKKVLIANRGEIVLRIMRTVREMGLESVVVYEKPDVDE
                     YFIRMADEAILIGDGPRKDYLDIERMIWAARKSGADAIHPGIGFLAENMDFSEACEKA
                     GITFIGPTADVIRNTGNKVAARELMEKAGIPVIPGTKALPSGSAAVKEVQAFGREHGY
                     PIMLKSPLGGGGRGIRRVRSEADLIGRLLRVRSEARKAFRDESIYVEKCIEAPRHVEI
                     PMLADRHGNIVQLASRDCSIQRRHQKLIGIAPANIDSNLLESLREAAVTAVRAAGAVN
                     AVTVEFLVDERDRKFWFMEINTRLQVEHVATEELINIDIVREQIRIAQGYRLSIPPER
                     TAFTGKAIQVRINCEDPRNKFMPDGGKVVTLYLPPGGPGIRLDGMIYNGYRIPADYDS
                     LMVKMTVRAFNWEQAVNRLKNALDLFLIAGPKTTIPFYRALCDEPDFRAEKFDTTYIE
                     THPDLFRYLEPGRKITKLEQFLMDIYSSEIFPQGWL"
     gene            2878..>3159
                     /locus_tag="P5573_06340"
     CDS             2878..>3159
                     /locus_tag="P5573_06340"
                     /inference="COORDINATES: protein motif:HMM:NF019924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HRT27712.1"
                     /translation="MRTLMFGWEFPPYISGGLGTACFGITEGLTEIGAEVLFVLPRIS
                     GEAGETHVELIPASRYPSDEARWPDETTKPPGNLDFRTVDVALRPYLNEA"
BASE COUNT          680 a          941 c          853 g          685 t
ORIGIN      
        1 ccctctcgaa gcctgcggta ccagcggcat gaaggctgcc ctaaacggcg tgccacatct
       61 gagcattctc gacggctggt ggattgaggc gtacaacggc aggaacggct gggccttcgg
      121 cggcgaggtg gccggcgaga atcgcgaccc ggccgacgct gaggcaatct ataacctgct
      181 cgagagtcag atcatccctc tctactacga tgtcgcggaa aacggaacct ccccgggctg
      241 gctaaaagtg atgaaagaag cgatccaaag taacttcccc cggttcagcg cccgccgtat
      301 ggtgcgggaa tacaacgaaa agttctacac gccggccctg cggaaggcat gaacggtaaa
      361 ccttcagtca ttgacctttc tcacaaaata atagtcgtgg cagtcggggt ctccctcgac
      421 atccctcgtc ctttcgagga gttcgaggcc ggcggcttca aggaggagca ggaattcccc
      481 gggcgtgaat gaagtcatgt atatgacgtt ccttgcgtaa tcgtcatcct tttgctgaag
      541 ctttctggag ccgtcgaaat accgggtctg taatagggcg aaaccgcccg gcttcaacag
      601 cgtccccatg accttgagga cgcgggcggc gtacggtttt gacgggaaat gttggaagac
      661 gttgacggaa acgatgaagt ccacagatcc cgccgttcca ccgtcgagca ccgattcagg
      721 gtcatcggag ggaaaggcga tcccccggta attcagcagg ccgaggtatt ccatccgtct
      781 tacggactcg gcgaccgacg ccttcgagac gtctaccccg tagactgacg cgaaccgttg
      841 gcacaacggc cggacgacgg cgccgccgcc gcaaccccat tccagggcaa ccaagcgatt
      901 caacccggcc acataatcaa ctccagcata ccgctcgagg tatttcaggg tgagtcggag
      961 atagaggtcg ccatgctcgt accatcggtc gctgtcccat cgcatcacac cacaccagtg
     1021 ggattggtcc tgtatcctcg cttccctgtc gctcatgccc cagtaacacc cggcgtcttt
     1081 ttccaggtcg ggtttctgtc tgagacggcg gaagccttcg ataatgcgct tgaacatccg
     1141 atgatgaacg tttaaagcca accttgggga aaaatttccg acgagtaaat atccatcaga
     1201 aattgttcca atttggtgat cttgcggccc ggttcaaggt aacggaataa gtcgggatgc
     1261 gtctcgatgt atgttgtgtc gaacttttcc gccctgaaat ccggctcgtc gcaaagcgcc
     1321 ctgtagaagg ggatggtggt ctttggcccg gcgatcagga aaaggtcgag ggcgttctta
     1381 agcctgttga cggcctgttc ccagttgaag gcccgcaccg tcatcttgac catcagagaa
     1441 tcgtaatccg ccgggatacg gtatccgttg tatatcatgc cgtccaatcg tatccccggc
     1501 cctcccggag gaaggtaaag ggttacgacc ttcccgccgt cgggcatgaa cttgtttctg
     1561 gggtcttcac agtttatcct gacctgaatc gcttttcccg tgaatgccgt ccgctccggg
     1621 gggatgctca accggtatcc ttgagcgatc cgtatctgct ccctgacgat gtcgatgttg
     1681 atcaattcct cggtcgcgac atgctccacc tgaagccggg tgttgatctc catgaaccag
     1741 aatttcctgt ccctttcgtc gacgagaaac tcgaccgtca cggcattcac ggcaccggcg
     1801 gcacgtacgg cggtaacggc ggcctcacga aggctctcca acaggttgga gtcgatgttc
     1861 gcgggggcga ttccgatgag cttctgatgc cgccgctgga tcgagcagtc ccgcgaggcc
     1921 agttggacga tgttcccgtg ccggtcggca agcatcggta tctccacgtg ccggggcgcc
     1981 tcgatacatt tttccacgta gatgctctcg tctctgaagg ccttacgggc ctcggaccga
     2041 acgcgcagca acctgcctat caaatccgcc tcgctcctaa ccctgcgaat gcccctgccg
     2101 ccgccgccca acggcgattt aagcatgatg ggatagccgt gttccctccc gaaggcctga
     2161 acttccttga cggcggcgct gccgctgggg agagccttcg tccccgggat taccggaatc
     2221 cccgctttct ccatgagctc gcgcgccgca actttattgc ctgtgttgcg gatcacgtcc
     2281 gccgtaggac cgatgaacgt gatgcccgcc ttttcgcagg cctccgaaaa atccatgttt
     2341 tccgccagaa aaccgattcc gggatgaatg gcatcggcgc cgcttttcct cgccgcccat
     2401 atcatccttt ctatatcaag ataatccttt cgcggcccgt cacctatcag aatcgcctca
     2461 tcggccatcc ggatgaagta ttcatccaca tccggttttt cgtaaacgac gaccgactct
     2521 agccccattt ctctgacggt ccgcattatt ctgaggacga tctcaccccg attggcgatc
     2581 aacacctttt ttatgctccg gttctctgtc atctatccgg caccacgacc cgtgttcata
     2641 atatgaacaa atcctaaaga aagagtagaa catatacctc agtctccctc cgggttcaat
     2701 gaaaaatccc tcacaactgc ggaagcaaga ccgccgccga ggaaggcggc aagacggaca
     2761 ggtcagacat atcgacaaag ggacgacctc acgacgaaag aagctgcgtt ggacaaatag
     2821 ggcgtttttc tggtagaagg taagaaattc cgccccggtg aaggagagtc ctttcccatg
     2881 cgcaccttga tgtttggctg ggaatttccc ccgtacataa gcggaggtct cggcacggca
     2941 tgcttcggca tcaccgaagg actcacggaa atcggtgcag aggtcttgtt cgtcctgccg
     3001 agaatctcgg gtgaagccgg cgaaacacac gttgaactca ttcccgcttc cagataccct
     3061 tctgacgaag cgcggtggcc agatgaaacg acgaagccac cggggaacct tgacttccgg
     3121 acagtcgacg ttgcgttgag gccgtacttg aacgaggcc
//
LOCUS       DAOTMX010000203         3150 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_24192_length_3150_cov_3.390953, whole genome shotgun sequence.
ACCESSION   DAOTMX010000203 DAOTMX010000000
VERSION     DAOTMX010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24192_length_3150_cov_3.390953"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..681
                     /locus_tag="P5573_06345"
     CDS             136..681
                     /locus_tag="P5573_06345"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HRT27713.1"
                     /translation="MVEKAELDILEIESPNEAELPGEEALPEDEPGSGGEKKRPLRVI
                     VLVSLALVVCAAAFAWLYFRGDLAGDRTKKTPPAVSAEGTFVSLDNFAVNIKDDKGNY
                     RILACDVALELNAGVDAARERILDLRKAVYRTVRNSGSYVLQDLFKARRQLLKDIERE
                     ANGVLGEGAVKQVYFTKYTLL"
     gene            735..1067
                     /locus_tag="P5573_06350"
     CDS             735..1067
                     /locus_tag="P5573_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27714.1"
                     /translation="MGRIGIAFFVVFWVFLNYSYALTPEQIIKLKQAGVEDKTIRMMI
                     QQEMEAKERDGATDSIGMREIKDEEGNTVIVYSSGKPGGCEPCDPEQEKVDKAWRMLQ
                     NIIIDKRK"
     gene            1090..1857
                     /locus_tag="P5573_06355"
     CDS             1090..1857
                     /locus_tag="P5573_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27715.1"
                     /translation="MRHEDGESLQEVACKLRAAFEASFVREMMPGVVHNFANPLNGIM
                     GRSSLVQRRMETHIKRMSRGVEVSLESAAGMMKDIEIISGVTDRLTEMLHVLSEKHQA
                     VNDKTEQRINLSKLLSLEARFFEFDLDFKHNIKRTLKLEEEIPDVMGCPADLSLCLSA
                     IMRHVVRETDGQPVREVFLSTGCEGGFVTMEVAFRCEGDAGMHKYLLDGVDAIYKDVP
                     DISLGFFLLTRKYGAQVRVVTADGWVRYLIGIPCPEA"
     gene            1939..2250
                     /locus_tag="P5573_06360"
     CDS             1939..2250
                     /locus_tag="P5573_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27716.1"
                     /translation="MTGPLDIKQVLLQINSVERVQQVQQQHPEMQQRYLDIQLGEEKK
                     LLKENVSHADNTNKTEEKRIREREKLGRKSGDRREGRKGRPDEGEGEDGAPGGHVDVR
                     V"
     gene            2252..2725
                     /locus_tag="P5573_06365"
     CDS             2252..2725
                     /locus_tag="P5573_06365"
                     /inference="COORDINATES: protein motif:HMM:NF022428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2802 domain-containing protein"
                     /protein_id="HRT27717.1"
                     /translation="MNITLLLTLQLVADAVLCIAVVFLFAVVNREIKRRGGGVDPKAL
                     GEFSRLISQSQGATENLLRAMEESRKILKEISFAVEEKEKRLRVLMEATEVRLSALTG
                     REGEEGAGESDNRYEKAVDLIGQGLTEGEVAERLGLTEGEVSLIVELKRKKDEPA"
     gene            2712..>3150
                     /locus_tag="P5573_06370"
     CDS             2712..>3150
                     /locus_tag="P5573_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27718.1"
                     /translation="MSPHRVDPPKAVLLQEASLRTSSAHQVRSLDLVVGELLTADVLE
                     ARDGKQYLVSLKGSTLTADSEVVLKPGDRVVVRVDQQFPRVVLSLIEVPPKTSLLQSD
                     LLRWYRSNPGALRDMFSEAAEVFSREKLGGLVRYIGRNDVKDAL"
BASE COUNT          777 a          732 c         1018 g          623 t
ORIGIN      
        1 gacgatgaag gaaatcggac aggtgatgag tctgacggag tcgagggtct gtcaactcca
       61 ttcgagggcc gtcttgcggc tccgcggccg gctgaaggat atccgggatt gagataagtc
      121 gaggagtcag acatcatggt ggaaaaggcg gagctggata tcctggaaat cgaatcgcct
      181 aatgaggcgg aactgccggg tgaagaggcg ctcccggaag acgagcccgg ttcgggcggg
      241 gaaaagaagc gtccattgag ggtcatagtg ctggtctcgc ttgccctggt ggtttgcgca
      301 gcggcattcg cctggctgta ttttcgcggc gaccttgccg gcgacaggac aaaaaaaact
      361 cctcctgccg tctcggcgga ggggactttt gtttctctgg ataattttgc cgtcaacatc
      421 aaggacgaca aggggaatta tcgcatcctg gcctgcgacg tcgccctgga attgaacgcc
      481 ggggtggacg cggcacggga gaggatactg gacctcagga aggccgtcta cagaacagtc
      541 cgcaacagcg gttcttacgt acttcaggat ctgtttaagg ccaggcgaca gttactgaag
      601 gatatcgaaa gggaagccaa cggcgttctg ggggagggag cggtaaaaca ggtttatttt
      661 acgaagtata ccttactctg aaggacgttg gacgatgagg cgtcgttggg aaaacgcgac
      721 gccgggaggg ggacatggga aggatcggaa tcgcgttttt tgttgtcttt tgggttttct
      781 tgaactatag ctatgctctg accccagagc agatcatcaa gctgaagcaa gcgggcgtcg
      841 aggataagac tatccggatg atgatccagc aggagatgga ggccaaggag agggacggcg
      901 ccaccgactc gatcgggatg cgggaaataa aggacgagga aggcaatacg gttatcgttt
      961 actcttcggg caagcccgga ggatgcgaac cctgtgaccc ggaacaagag aaggtggaca
     1021 aggcatggag aatgctccag aacatcataa tcgataagag gaagtaggag cgggagaaac
     1081 aggaaaacga tgagacatga agacggcgaa tccctgcagg aggtcgcgtg caaactccga
     1141 gcagcctttg aagcctcttt cgtgcgggag atgatgcccg gagtcgttca caattttgcc
     1201 aatcccctca acgggatcat gggacggtcg agccttgtcc agagacggat ggagacccac
     1261 ataaaaagga tgtcgagggg ggtggaggtc tctttggaat ccgccgccgg tatgatgaaa
     1321 gatatcgaga tcataagcgg agtgaccgac cggcttacgg aaatgctgca cgtgctctcc
     1381 gagaagcacc aggcagtgaa tgacaagact gaacagagga taaatctgtc gaaactcctt
     1441 tccctcgagg cgcgcttctt cgagtttgac cttgacttca agcacaacat caagaggacg
     1501 ctcaagcttg aagaggaaat tcccgacgtg atggggtgcc cagccgattt aagcctctgt
     1561 ttgtcggcga tcatgaggca tgttgttcgg gagacagacg gccagcctgt ccgcgaggtc
     1621 ttcctgtcga cgggatgtga gggcggtttt gtgactatgg aggtcgcttt cagatgtgag
     1681 ggcgacgccg ggatgcataa atatctgttg gacggagtcg atgcgatata caaagacgtg
     1741 ccggatatat ccctcggttt ttttctactc acccgcaaat acggggcaca ggttcgggtt
     1801 gtcacagcgg acggatgggt caggtatctc ataggtatac cctgtccgga agcctgaaat
     1861 taaagaaacg gccggtgaat gacgattatg atggataagg gtgggtagtt cgacccctgt
     1921 gagtcgggag gtagggtcat gacggggccg cttgatatca agcaggtttt gctgcaaatc
     1981 aactccgtgg agcgcgtgca acaggttcag cagcagcatc cagagatgca gcagcgatac
     2041 ctcgacattc agctcggcga agagaaaaaa cttctcaaag aaaatgtcag tcacgcggac
     2101 aatacgaata aaacagaaga aaagagaatc cgggaacggg aaaaattagg cagaaaaagc
     2161 ggcgaccggc gggaaggccg aaaagggcgt cctgacgagg gtgaaggcga ggacggtgcg
     2221 ccgggaggcc atgtagacgt cagagtatga catgaacatc acattgttgc tgacgctcca
     2281 gttggtagcc gacgcagtcc tatgcatagc cgtcgtgttc ctttttgcgg tcgtgaaccg
     2341 tgagatcaag agacggggcg gtggagtcga cccgaaggcc ttaggggagt tcagccggtt
     2401 gatctctcaa tcccagggcg ccaccgagaa cctgctcagg gccatggaag aaagccgcaa
     2461 gatcttgaag gagatatcct tcgccgtcga ggagaaggaa aagcgtttgc gggtactcat
     2521 ggaggcaacc gaggtcagac tgtctgctct gacaggccgg gaaggggaag agggggctgg
     2581 tgaatcggac aaccgctacg aaaaggccgt cgatttgatc ggacagggcc tgacggaggg
     2641 tgaagtcgcc gagaggctcg gtttgaccga aggtgaagtt agcctgatag tggaattgaa
     2701 acgcaagaag gatgagcccg catagagtcg accccccgaa ggccgttctc ctgcaggaag
     2761 catcgctcag gacctcttcg gcccaccagg tccggtccct cgaccttgtc gtgggtgaac
     2821 tgctgacggc agacgttctt gaggcccggg acggcaagca gtaccttgtt tccctgaaag
     2881 gttcgaccct cacggccgat tcggaggtgg tgctcaaacc gggagacagg gtcgttgtcc
     2941 gggtcgatca gcagtttccc cgcgtcgtgt tgagtctcat cgaagtcccc ccgaagacat
     3001 ctttactcca gagtgatctg ttgaggtggt accgctccaa ccccggggcc ctccgcgata
     3061 tgtttagtga ggcggcggag gtgttcagcc gtgaaaaact cggagggctt gtccgctata
     3121 tcgggcggaa cgatgtcaag gatgctctca
//
LOCUS       DAOTMX010000204         3098 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_24683_length_3098_cov_3.452843, whole genome shotgun sequence.
ACCESSION   DAOTMX010000204 DAOTMX010000000
VERSION     DAOTMX010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24683_length_3098_cov_3.452843"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="P5573_06375"
     CDS             complement(<1..495)
                     /locus_tag="P5573_06375"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRT27719.1"
                     /translation="MILESKGKIAEGLYAVGVAELPAYLLVGKTPALFDAGMTFMGPR
                     YLGEVREVIGDAARLGFNFLTHSHYDHCGSVPYLKRRIPDLKVGASRLAADIFQRRGA
                     VDLIRSLSKDYEERYRETVGGEDVSFDGLTVDIPLDDGAEMELGGGFGFRAIATPGHT
                     RDSIT"
     gene            906..2090
                     /locus_tag="P5573_06380"
     CDS             906..2090
                     /locus_tag="P5573_06380"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRT27720.1"
                     /translation="MTVSNKIEGFINQSSWIRKMFEDGLRMKAQYGEDRVFDFTLGNP
                     NLEPPPEFHRLLREIASMPIPGKHAYMPNAGYPETRKSVAEHVSKEHGVELSADEIVM
                     TCGAGGAINVILKALLDPGDEVVVPAPFFVEYRFYADNHGGVLRPVRTREDFSLDMQE
                     MDKAVTDKTKVVMINSPNNPTGKVYDEATIRELAALLEEKQRVYGKALYLVSDEPYAQ
                     IVYDGVVVPSVLKAYENSIIATCYSKSVSIPGERIGYIAAHPGLTDLRRVMEGFILSN
                     RILGFVNAPALMQRVVARLQGAIVDVGEYKRKRDLLCRGLSAMGYEFEEPKGAFYLFP
                     KSPTGDDVEFVLRLQEKNILTVPGSGFGGPGHFRISYCVDDATIVKAMPGFEQALKEA
                     RA"
     gene            complement(2134..>3098)
                     /locus_tag="P5573_06385"
     CDS             complement(2134..>3098)
                     /locus_tag="P5573_06385"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HRT27721.1"
                     /translation="HLGYDSEGNNDLAIVWLDASAPTDADRYGIYRESDEVGQKFTAV
                     GYGRTGTGKTGATDYNADYIRLKAGNRFDADASVLKSYLGSSMAWTPTPGTQLIADFD
                     NGKSANDALGQLIHRSDLGLGPDEGLIAPGDSGGPAFLKGQIAGIATYYASLSEGSTH
                     PDVDNRLNSSFGEIAAWQRVSTYQQWIDQSLRANYQNAPTKPEDVKKEVAEGDSGTTY
                     TYFLLQFIGIRSKPDEILSVDYATHDGTAKAGSDYIAASGRLNLYPGENQAVIPVEIV
                     GDTTPEPSETFSLDVFNPVGGSFGEGVVKLTAVRTIIDDDGWVG"
BASE COUNT          625 a          894 c          925 g          654 t
ORIGIN      
        1 cgttatcgag tctctcgtgt ggcccggtgt cgcgatcgcc ctgaaaccga agcctccgcc
       61 cagttccatc tcggcaccgt cgtcgagagg aatgtcgacc gtaaggccgt cgaatgatac
      121 atcttctccg ccgaccgttt ccctgtatct ctcctcgtaa tccttactca aactacgaat
      181 caagtcgacg gcgccccgcc tctggaagat atccgcggcg agacggctcg cccctacttt
      241 gaggtccggt atgcgtcgtt tgagataggg cacggagccg caatggtcat aatgggaatg
      301 ggtcaggaaa ttgaatccaa ggcgggctgc gtcaccgatg acctccctta cctctccgag
      361 atagcgcggt cccatgaagg tcatgccggc atcgaacagg gcaggcgtct ttccgactaa
      421 aagataggcc ggaagttcgg cgaccccgac agcgtacaga ccttcggcga tctttccttt
      481 actttccagt atcatgcgtt ttttggtgga gccgggttat gaggcggccg ccggccgccg
      541 gatgctattg ccgtcacgta aaatcggagc acgttgcggc acaggggggc gccgacggca
      601 tatccttccc gactttgctc ccgaagacgg acaagaccct ctccgcctct tccgaacggc
      661 agtcggggca cgaaatattc agccgttcgt gctgcgtacg gaataagact tcgaagactt
      721 ttccgcagtt cttacacttg aactcataga tgggcatcag ttgtttcacc tcatgcgtgg
      781 ccttcttagc ggttttttag aggataacat gaccgatgtc aagggttaat gggccgggtt
      841 ttattgactg cgcctgtggg tttatgctat tgcgaaccgc ataccatgac aaaggagagc
      901 cgacgatgac tgtttcaaat aaaatagagg ggttcatcaa tcagtcttcg tggatccgga
      961 agatgttcga agacggcctc aggatgaagg cccagtacgg agaggaccgg gttttcgact
     1021 tcaccctggg gaaccccaat ctcgagccgc ccccggagtt ccatcgcctg ctgcgcgaga
     1081 tcgcttcgat gccgatccca ggaaagcatg cctacatgcc caatgcgggg tacccggaaa
     1141 cgaggaaatc cgtcgccgaa cacgtgtcga aagagcacgg cgtcgagctt tccgccgacg
     1201 aaattgtcat gacctgcggg gctggggggg cgataaacgt catactcaag gcactcctcg
     1261 accccggcga tgaggtagtg gtccccgccc cgtttttcgt cgaataccgc ttttacgcgg
     1321 acaatcacgg cggagtgctc cgtcctgtga ggacgaggga ggatttctcc ctggacatgc
     1381 aggagatgga taaggccgtc acagacaaga cgaaggtggt catgatcaat tccccgaaca
     1441 accccacggg aaaggtctat gacgaggcga cgatccgtga actggcggcc ctcctggagg
     1501 aaaagcagag ggtttacggg aaagcgctat acctggtgtc cgacgaaccg tacgcccaga
     1561 tagtatacga cggcgtcgtc gttccgagcg ttctgaaggc atacgagaac agcatcatcg
     1621 ccacgtgcta ttcgaagagc gtctccatcc cgggagagag gatcgggtac atcgcagccc
     1681 atcccggcct tacggacctg aggcgcgtca tggaggggtt catactcagt aacaggatcc
     1741 tcgggtttgt aaacgccccc gcccttatgc aacgggtcgt ggcgagactc cagggcgcaa
     1801 tcgtcgatgt gggagaatac aagaggaaaa gagacctcct gtgccgggga ctgtcggcga
     1861 tggggtacga gttcgaggaa ccaaaaggcg ccttctacct attcccgaag agcccgacag
     1921 gggacgacgt cgaattcgtc ctcaggttgc aggaaaagaa catcctcaca gtccccggga
     1981 gcggcttcgg cggccccggt cacttccgga tttcctactg cgtcgacgac gcgacgatcg
     2041 tcaaggcgat gccggggttc gaacaggccc taaaggaggc gcgagcgtag atgccattcg
     2101 gcgtcgagcg taagcagctt tcggccttcc gattcatccg acccagccgt cgtcgtcgat
     2161 gatcgtgcgc accgccgtca gcttgaccac cccctcgccg aagctcccgc ctaccgggtt
     2221 gaaaacatcc aaggaaaagg tttcgctggg ttccggggtc gtgtcaccga cgatttctac
     2281 gggaatcacg gcctgattct cgccgggata gaggttcagc cgaccgctcg cggcgatgta
     2341 gtcgctcccg gccttggccg taccgtcatg cgtggcgtaa tcgacgctga ggatctcgtc
     2401 gggtttgctg cggatgccga taaactgcaa gaggaagtaa gtgtaagtag tgccgctgtc
     2461 accctcggcg acctcttttt tgacgtcctc gggcttggta ggtgcgtttt ggtagtttgc
     2521 cctcagactc tggtcaatcc attgttgata ggtactcacc cgctgccagg cggcgatctc
     2581 gccgaaactg ctgttcagtc tattgtcgac atcgggatgg gtagatcctt cactcaggct
     2641 ggcgtagtag gtggcgatgc ccgctatctg ccccttgagg aaggccgggc ctccactgtc
     2701 gccgggagcg atcaagccct cgtccgggcc caggccgagg tcactgcggt ggatcagctg
     2761 accgagtgcg tcgttggcgc tttttccgtt gtcgaaatcg gcgataagtt gtgtgccggg
     2821 ggtcggcgtc caggccatgg acgaccccag atagctcttt aggaccgagg catccgcgtc
     2881 gaaccggttg ccggccttga gccggatata gtcggcgttg tagtcggtgg caccagtctt
     2941 ccccgtgccg gtcctaccgt atccgaccgc cgtgaatttc tgaccgacct cgtcgctttc
     3001 gcggtagatg ccgtagcggt cggcgtcggt cggcgccgag gcgtcgagcc agactatcgc
     3061 aaggtcgtta ttgccctccg agtcataccc caggtgca
//
LOCUS       DAOTMX010000205         3089 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_24769_length_3089_cov_4.049769, whole genome shotgun sequence.
ACCESSION   DAOTMX010000205 DAOTMX010000000
VERSION     DAOTMX010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24769_length_3089_cov_4.049769"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /locus_tag="P5573_06390"
     CDS             complement(<1..860)
                     /locus_tag="P5573_06390"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRT27722.1"
                     /translation="MIDIERLEKVRLVRKPLGQRIIGALLLTVNYRVFADVDIRIENI
                     ERIPKDETVIFAMNHTDRYNYWPFQWKLWRKKCFPFTTVWVKGKYYRNALLGKFLDWC
                     NLIPVPSMGYLVEEFYRKRFKRRIEKEEYRVIKDLIDGKQRLSEALKKLTPETAAMLK
                     DDFVEHVRNYHEILMTRVAELSCKALLEKNLNLIIFPEGTRSLKLGEGKTGLAQLALH
                     TEKAVVPVGCNNSDLVYRGHLPFARSGRITYRVGEPLSFNGQLKDYRIREGFKLFSRE
                     SQRRYREQFEA"
     gene            complement(1107..1382)
                     /locus_tag="P5573_06395"
     CDS             complement(1107..1382)
                     /locus_tag="P5573_06395"
                     /inference="COORDINATES: protein motif:HMM:NF027875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen-binding domain-containing protein"
                     /protein_id="HRT27723.1"
                     /translation="MVERIAKRRVDFEYYSPEAKSVFLAGTFNGWNTAANPMKRNKEG
                     RWSTFVNLYPGFYEYQFVVDGAWRDDPACKKRHLNKYGGYNSVLVVE"
     gene            1760..>3089
                     /locus_tag="P5573_06400"
     CDS             1760..>3089
                     /locus_tag="P5573_06400"
                     /inference="COORDINATES: protein motif:HMM:NF014594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HRT27724.1"
                     /translation="MTNKSTDWKERIVAPEKALAAIEPGMSIFVGTGMAEPRTLVKHL
                     MTSGESNLQDLEMIQLVSLGDTVSIDERYSKKYRLKTFYSGWVASTAITAGRVDLIPS
                     RFSRIPWLFKSGAIRIDAAFIQITPPDETGHAGLGVGVDVARYALEQAAVVIGEVNES
                     VPRTLGDTLVRLDEFDFFVQSAEPLLYIPRWAVPDVYRKVAENVAGIVEDGSCISFSI
                     GPLYEALGKELARKRHLGIHTPFFTDALMDLVKTGAVTNRLKSFYRGKSLTSYALGTP
                     ELMKWLDRNPLVEFQPLDVVAEPQNMGANDRLIAVLPARKADLTGGIALHMGMGNVTA
                     GPGAVQELFAGAALSRGGRTIFALPSRNRSGDANIRLSVEDYPNQFSNRESLDIVVTE
                     YGVAYLTGRTVRERAQALIDIAHPDDRADLVRQAKEARLLYPDQVFFPESG"
BASE COUNT          695 a          920 c          802 g          672 t
ORIGIN      
        1 gcttcgaatt gttccctgta cctacgttgc gactctctcg aaaagagctt aaagccttcc
       61 cgtatcctgt aatccttcaa ttggccgttg aaggagagcg gttcccccac ccggtaggtt
      121 atccggccgc tccttgcgaa agggaggtgc cccctgtaca cgaggtcgga attgttgcaa
      181 ccgacgggaa cgacggcctt ttccgtatgc agggcaagct gggcgagtcc cgtttttccc
      241 tccccgagct tcagagaccg cgtcccctcg gggaatatga tcaggttgag gtttttttcc
      301 agaagtgcct tacagctcaa ctcggctacc cgggtcatca aaatttcatg gtaattcctg
      361 acgtgctcga cgaaatcatc cttcaacatc gccgccgtct cgggagtaag ttttttcagg
      421 gcctcgctca agcgctgttt cccatcaatc aagtccttga tgacccggta ctcttctttc
      481 tcgatccgcc ttttgaatcg ttttcggtaa aactcctcga caagatagcc catcgaaggt
      541 acggggatga gattgcacca gtctaagaat ttgccgagca gggcgttgcg gtaatactta
      601 cctttcaccc aaacggtagt aaagggaaaa cacttcttcc gccataactt ccactgaaaa
      661 ggccagtagt tgtaacggtc tgtgtggttc atcgcgaaaa tgaccgtttc gtccttgggg
      721 attctctcga tgttttcgat gcggatgtcg acgtcggcga aaacgcggta attgactgtc
      781 agcagaagcg caccgatgat gcgctggccg aggggctttc tcacgaggcg aaccttctca
      841 agacgctcga tgtcaatcat aacgactcca gctttttgat gggccaacaa tatgcccggc
      901 ctcttcggat gtcaatccca aaataagcac aaaaacgggc gctcttacgc gtaggaatgc
      961 gtcagcacat acccccttca ggtacaagtt ccgtaatccg aacaatacgg attggatgga
     1021 acatcacgta tactgccagg gctcccagcc ggctccgagg acggcccgac tgggaccagg
     1081 ccgctactgg tgatgtcctc ggacggtcat tcgacaacga gcacagaatt gtagcctccg
     1141 tatttgttca gatgacgctt cttgcaggcc gggtcgtccc tccacgcgcc gtcaacgaca
     1201 aattgatact cgtaaaaacc aggatagaga ttgacaaaag tcgaccacct accctctttg
     1261 ttccttttca tcgggtttgc cgccgtattc cagccgttga aagtcccggc cagaaagacg
     1321 gacttcgcct ccggtgaata gtactcaaaa tcgactcttc tcttcgcgat cctctcaacc
     1381 atacctgcac ctccttccat gagaaacaat tcttgtgggt tattctttcg ggataacagt
     1441 gcagtcgcct gtcagatcgg cgctcggtga aaacctacct acgacacagc aggggagaaa
     1501 atcagaaact ctatcggcct aacggccaca gacgtgaccg taacttatag aaacggataa
     1561 catgttttcg atttttcttc aacccttatc gtcgatgtcc cgcaaacatc agctgttgaa
     1621 aaagcgacgt ccgcagtttg ccatcgggtg gggatgccct gagtctatcg aagaggggct
     1681 ctttcaacgg atgttgccat aatttaccgc ctgctgtata aaaacccaca caggcaatga
     1741 cacctccaag gctgccccga tgacgaacaa aagcaccgac tggaaagaac ggatcgtcgc
     1801 gcccgagaag gcgctcgccg ctatagagcc gggaatgagc atcttcgtcg gcacgggcat
     1861 ggccgaaccc cgaaccttgg tcaagcacct catgacgtcc ggggaaagca atctccagga
     1921 cctcgagatg atccagcttg tgagcctcgg agacaccgtt tccatcgacg aacgctactc
     1981 gaagaaatac cgcctcaaga cattttactc cggttgggtc gcaagcacgg ccatcacggc
     2041 aggccgcgta gaccttattc ccagcaggtt ttcccgcatc ccctggctct tcaagtcggg
     2101 ggcgatacga atcgatgccg ctttcatcca gataacgccg cccgatgaaa cgggtcatgc
     2161 cggtcttggg gtgggtgttg acgtggcccg ctacgccttg gagcaagccg ccgtcgtgat
     2221 cggggaggtc aacgagagcg tgccccgcac actcggagac acattggtcc gcctggatga
     2281 gttcgacttt ttcgttcagt ccgcggaacc gctcctgtac ataccgcgct gggcagtccc
     2341 cgatgtgtac cgaaaagtag ccgagaacgt cgccggcatc gtcgaagacg gaagctgcat
     2401 ctccttttcc atcggacctc tttacgaagc gctgggaaaa gagcttgcgc gcaagcgcca
     2461 cctcggcatc catacgccct tcttcacgga cgccctcatg gaccttgtca agacaggggc
     2521 agtcaccaac cgcttgaaga gtttttaccg cggcaaatcc cttacctctt acgcactggg
     2581 gacgcccgaa ctgatgaaat ggctcgaccg taacccgctg gtcgagttcc agcccctcga
     2641 cgtggtggct gagccgcaaa acatgggagc caacgaccgt ttaatcgccg tccttcccgc
     2701 caggaaggcc gacctcacgg gggggatagc cctccatatg ggcatgggta acgtaacggc
     2761 aggtccgggc gcagtgcagg agctgttcgc aggggcggcg ctctcacgag gaggccgcac
     2821 gatctttgcc ctcccgagcc gtaatcgctc cggggatgct aacatccgcc tgtccgttga
     2881 agactatccc aaccagttct ccaaccggga atctctggac atcgtcgtca ccgaatacgg
     2941 cgtcgcctat ctcacgggcc gcacggtgcg cgagcgggcc caggccctca tcgacattgc
     3001 ccaccccgat gatcgggcgg acctcgtgag gcaggcgaaa gaggcaagac tcctctatcc
     3061 cgaccaggtg ttcttccccg aatcgggac
//
LOCUS       DAOTMX010000206         3081 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_24867_length_3081_cov_3.712161, whole genome shotgun sequence.
ACCESSION   DAOTMX010000206 DAOTMX010000000
VERSION     DAOTMX010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24867_length_3081_cov_3.712161"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /gene="gltX"
                     /locus_tag="P5573_06405"
     CDS             complement(<1..634)
                     /gene="gltX"
                     /locus_tag="P5573_06405"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HRT27725.1"
                     /translation="MTTSENQVRTRFAPSPTGYLHIGGARTALFNWLYARHFGGKFIL
                     RIEDTDQERSTDESTRAIFESMEWLGLNWDEGPYHQAERKHLYQEAVRKLLEEGKAYR
                     CICTPEELEMKRKKALAEGRKPKYDGTCRDRNLDWRPGSVVRFRSPDAGLTTVNDLIK
                     GPVHFNNEELDDLIIERADGYPTYNFAVVVDDAQMGITHVIRGDDHLNNTP"
     gene            861..1397
                     /locus_tag="P5573_06410"
     CDS             861..1397
                     /locus_tag="P5573_06410"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HRT27726.1"
                     /translation="MLKIKVASISEEGLEYLLSDDGSRLLEIIPDKGKIDFTLHRVDV
                     RCFARKVGSTIPLQVKAETKVGLECGRCLEMVTYPVKMAFACSLAPTGAGIAAEDSAD
                     VGEGLNLGSYQDDVIDLAPLVLEQVVLQIPIKPLCREDCKGLCPRCGANLNETTCDCE
                     TEVVDQRFAILNKFKVLR"
     gene            1424..1609
                     /gene="rpmF"
                     /locus_tag="P5573_06415"
     CDS             1424..1609
                     /gene="rpmF"
                     /locus_tag="P5573_06415"
                     /inference="COORDINATES: protein motif:HMM:TIGR01031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HRT27727.1"
                     /translation="MPNPVKRHSKSRRNKRRAHDFLTPPITSLCPQCKQPKLPHQACL
                     SCGTYKGREVRSVEKAS"
     gene            1674..2417
                     /gene="fabG"
                     /locus_tag="P5573_06420"
     CDS             1674..2417
                     /gene="fabG"
                     /locus_tag="P5573_06420"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002442508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HRT27728.1"
                     /translation="MGLTGKVALVTGGSRGIGKAVAVRLAADGAYTFVNYARNEEAAE
                     ETLELIRGKGGSAELLSFDVADFAIVQRAVARIVEQKGRLDILVNNAGISVDGLLVRM
                     KEEDWDRVIAVNLKGVFNCCRAAARPMMRQRWGRIVNISSIVAKSGNAGQVNYCASKA
                     GVEGLTRSLARELGARNVCINAVAPGFIETDMTAFVQEEEREALRRQIPLARLGAPED
                     VAAVVRFLVSEEAGYVTGQVVHVNGGLYM"
     gene            2458..2703
                     /gene="acpP"
                     /locus_tag="P5573_06425"
     CDS             2458..2703
                     /gene="acpP"
                     /locus_tag="P5573_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005779510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRT27729.1"
                     /translation="MSTIEEKVIEIIMEQLGVTKEECVPEAAFIDDLGADSLDIVELI
                     MAMEENFGIEISDDELMKVRTVQDVIDFIKKKRGVLN"
     gene            2718..>3081
                     /locus_tag="P5573_06430"
     CDS             2718..>3081
                     /locus_tag="P5573_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HRT27730.1"
                     /translation="MTGLGAVSPLGNSVSETWDGICSARSGIGSITRFDTAGFMTRIA
                     GELKGFDPLPFVNAKELRRYDDFIIYSLACAGMALEDSGFKVDSRNAERVGVIIGSGI
                     GGLGTLEKAKETLIQHGPR"
BASE COUNT          701 a          745 c          927 g          708 t
ORIGIN      
        1 gcggtgtgtt gttcaggtgg tcgtcgccgc ggatgacgtg cgtgatcccc atctgggcgt
       61 cgtcgacgac gacggcgaag ttgtaagtcg gatagccgtc ggccctctcg atgatgaggt
      121 cgtcgagttc ttcgttgttg aaatgaacag gtcctttgat caggtcgttg acggtggtca
      181 agccggcgtc cggcgaccgg aagcgcacaa ccgacccggg tctccagtcg aggttcctgt
      241 cgcggcaggt cccgtcgtat ttcggctttc gtccttcagc caaggctttt tttctcttca
      301 tttcgagctc ttccggcgtg cagatacacc ggtaagcctt gccctcctca agcagtttgc
      361 ggacggcctc ctgatagagg tgcttccttt cggcctgatg atacggtcct tcgtcccagt
      421 tgagcccaag ccattccatg gactcaaaaa tggctcttgt cgattcatca gtggagcgtt
      481 cctggtctgt gtcttctatc cggaggatga acttcccgcc gaagtgcctg gcataaagcc
      541 agttgaacag ggctgtccgt gccccgccga tatgcagata acccgtcggt gatggtgcga
      601 acctcgttct aacctgattc tcggatgttg tcatacttgt gtccgtcctt tatgaagtta
      661 gctgtcttgt cagtgaaccc ggcaactctt gctgaagata aagccgcggg ttctcgattg
      721 tcaagtcggt tttctacatt ctgagaacat tccgataata ttccttgaca aggtgcaata
      781 attttaatta aatttcacgc ttacgtttat tacaggagca agagtcgggt cccgaaggga
      841 accgccgtaa gtaatgagcc atgctgaaga taaaagttgc aagcatttcg gaagaggggc
      901 ttgaatatct gctttccgac gatgggagca ggctcctcga aatcattccg gataagggga
      961 aaattgattt cacccttcac agggtagatg tgcgctgttt tgcaagaaag gtcggcagca
     1021 cgattcctct tcaggtgaag gcggaaacga aggtaggcct ggagtgcgga cgatgcctcg
     1081 agatggtgac ctacccggtc aagatggcct ttgcctgttc actcgcgccg actggagcag
     1141 gtattgcggc ggaggattcc gcggatgtcg gcgagggcct gaacttgggc agttaccagg
     1201 atgacgtcat cgacctcgcg ccccttgtcc tcgagcaggt cgtcctccag attccgataa
     1261 agcccctctg cagggaagac tgcaaggggc tgtgcccgcg atgcggggct aacctcaacg
     1321 aaaccacctg cgactgtgag acggaggtgg ttgaccaaag atttgcgatt ctgaataaat
     1381 ttaaggtact taggtagtta attacataca gaaaggttgt acgatgccaa acccagtcaa
     1441 acgacattcg aaatcgagaa ggaacaaaag gagagcgcac gatttcctca ctcctccaat
     1501 tacttcactt tgtccgcagt gcaagcaacc aaagctgccc catcaggcct gtcttagttg
     1561 cgggacatac aagggcaggg aagtgcggtc cgttgagaag gcttcctgat tcaaaaccag
     1621 aatcactttt tcatgggacc ggtcagtgaa atgctaaggc cgaaagatta tagatgggtc
     1681 ttacgggcaa ggtagcgctg gttacgggcg gttcgcgagg gatcggcaag gcggttgccg
     1741 tcagactggc ggcggatggt gcctacacct ttgtcaatta tgcgcgcaac gaagaagctg
     1801 ccgaagagac cttggaactg atacgtggta agggtgggtc ggcagagctg ttgtccttcg
     1861 acgtagccga ttttgccatc gtccagaggg ctgtagcccg tatcgtagaa caaaaaggtc
     1921 gattggatat cctggtcaat aacgccggga tttccgtcga cggcctcctc gtgagaatga
     1981 aggaggaaga ctgggaccgg gtaatcgccg tcaatctcaa gggagttttt aactgctgca
     2041 gggcggcggc gcgaccgatg atgaggcaac ggtgggggag aatagtgaac atctcttcca
     2101 tcgtcgcgaa atccgggaac gccggtcagg tcaactactg tgcttccaaa gcaggagtcg
     2161 agggactcac gcgttccctg gcaagggagc tcggtgcacg taatgtgtgc atcaacgcag
     2221 tggcgccggg attcatcgaa accgacatga ccgcttttgt gcaggaggag gaacgggagg
     2281 ccttgaggcg ccagattccg ctcgcgaggc tgggagcgcc ggaagatgtg gcagccgtag
     2341 tgcggtttct tgtctccgag gaggcaggtt atgtgaccgg acaggtcgtt catgtaaacg
     2401 gcggtcttta catgtagcaa atcatgagtt gtgcactaat tcaaggagga catttcgatg
     2461 tcaacgatcg aagaaaaggt aatcgagatt atcatggagc aactgggtgt tacgaaggaa
     2521 gaatgtgttc cggaagctgc ttttatagac gatctgggcg ccgactctct cgatatagtg
     2581 gaactgatca tggcgatgga ggaaaatttc gggattgaga tctccgacga tgaactgatg
     2641 aaggtcagga ccgttcagga tgtcatagac ttcatcaaga aaaagagggg ggtgctgaac
     2701 tgaagaggag ggtcgtcata accggcctcg gtgccgtcag tcccctcgga aattcggtca
     2761 gtgagacctg ggacggtata tgttcggccc gctccggcat agggagcatc accaggttcg
     2821 atacggcggg ttttatgacc aggatcgccg gggagctgaa gggcttcgac cctctccctt
     2881 tcgtgaacgc gaaggagctc cgccgatatg acgatttcat catctattcg ctggcctgcg
     2941 cggggatggc cctggaagat tcggggttca aagtcgacag tcgaaacgcc gaacgggtcg
     3001 gcgttatcat cggttccggt atcgggggcc tcggcacctt ggagaaggca aaagaaaccc
     3061 tgatccagca cgggccgagg c
//
LOCUS       DAOTMX010000207         3071 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_24969_length_3071_cov_3.963528, whole genome shotgun sequence.
ACCESSION   DAOTMX010000207 DAOTMX010000000
VERSION     DAOTMX010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24969_length_3071_cov_3.963528"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="P5573_06435"
     CDS             complement(<1..228)
                     /locus_tag="P5573_06435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRT27731.1"
                     /translation="MNKKRDALIMQYAPLVKNIVERIAARVPPYIAAKEDLINVGVIG
                     LISAIEKFDKERNVQFETYARYRIRGAVLDEL"
     gene            complement(221..1126)
                     /locus_tag="P5573_06440"
     CDS             complement(221..1126)
                     /locus_tag="P5573_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HRT27732.1"
                     /translation="MDQAAQLRKMKVKAEMTGVAPSEYKAVEKQRNSGIRVLAVTSGK
                     GGVGKTHVSANLAYSLARLGKRVLVFDADMGLANIDIILGLTPRYNLHHVLQGKKTLR
                     EVLITGPGDIGILPAASGIKELADLTRGQKMTLIEELDNLDERYDYMIIDTAAGITGN
                     VLYFNMAAKEIVVVVSPEPTSITDAYALIKILYQSHAEKRIMLLVNMARSYQEAREVY
                     RRVSSATEHFLDLTIEYLGYVMYDERVADAARLQKALVEAYPGARASQCLMGIARKLS
                     REPAGRYNIGSMKFFSNAIIGRDCE"
     gene            complement(1142..2284)
                     /gene="flhF"
                     /locus_tag="P5573_06445"
     CDS             complement(1142..2284)
                     /gene="flhF"
                     /locus_tag="P5573_06445"
                     /inference="COORDINATES: protein motif:HMM:TIGR03499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HRT27733.1"
                     /translation="MQIKRYEAKNIQDALTRIKADMGEDAVILSTKRLSGGRQPLFEV
                     MAARDDRESLPSPGRGTAGKVEREGDKPDGVERLAGRIEDLTVLVREVREGAAIRREL
                     TDLKNTVNTLFDLLDLRDREKENHSGVFYHLISNGISSERARRLMSLVRKRTPVGPGE
                     EAEQLAAVEELIRASIKPPPREVQGQRTLAFVGPTGVGKTTTIAKLAARYALTEKRSV
                     GLITTDTYRIAAVEQLKVYGKIMGLSVEVAGDGVAFRNALKKFSDKEAVLIDTPGRSR
                     KDGNHLLKLKGIIRDAPVETHLLLSPTASREHMLDAAERFGIMGYGSIIFTKIDECTR
                     FGPIYDIVDQVGKPVSYLTNGQNVPQDIEVAEPDKIARLIVRNRLN"
     gene            complement(2289..>3071)
                     /locus_tag="P5573_06450"
     CDS             complement(2289..>3071)
                     /locus_tag="P5573_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HRT27734.1"
                     /translation="HYLVIAADDRGLKLKGIETKEPAFGLPAVWVGEKEKDGAQAKGY
                     VVVDPATVVTTHLTEVIKSHADELLRREEVRSLLDSLAANHPKVVEELVPNVLPLGLI
                     QKVLQRLLRERVPIRDLLTILETLADYVNLTKNVDILTGYVRQALARTITKQYRDAKG
                     DITVVTLAPALEEAISRAVQHTEYESYVAADPDLIQKVVTGLQRYIPQFTSRGLQPVV
                     LCSPQTRIHFRNIVERFFPNLAVISHNEVTRDTNVKSLGMVE"
BASE COUNT          527 a          952 c          838 g          754 t
ORIGIN      
        1 cagctcgtcc agcacggctc cccgtatccg gtacctggca taggtttcga actggacatt
       61 cctctccttg tcgaacttct ctatggccga gatgagtccg atgacgccca cgtttatcag
      121 gtcttccttc gccgcaatgt aggggggcac cctcgcggcg atcctctcca cgatgttctt
      181 gaccagtggg gcgtattgca tgatcagggc gtcacgcttc ttattcacag tctctaccga
      241 ttatggcatt cgagaagaat ttcatgctcc cgatgttata acgaccggcg ggttcccggc
      301 tcagtttccg cgcgataccc atgaggcatt gactcgccct cgcccctggg taagcctcga
      361 ccagggcctt ctgcagcctc gcggcatcgg ccactctctc atcgtacatg acgtacccca
      421 ggtactctat cgtcaggtcg agaaaatgct ccgtagcgct gcttaccctc ctgtagacct
      481 ccctcgcctc ctggtaactc cttgccatat tcacgaggag catgatacgt ttctcggcat
      541 gactctggta aagtatcttt atcagggcgt aggcatctgt tatggacgtc ggctccggtg
      601 agacgaccac aacgatttcc ttggccgcca tgttgaagta caagacgttg ccagtgattc
      661 ccgccgcggt gtcgatgatc atgtaatcgt accgttcgtc gaggttgtcc agttcctcga
      721 tgagggtcat cttctggccc cgtgtgaggt cggcgagttc cttgatgccg gaggccgcgg
      781 gcaggatgcc gatgtctccc ggcccggtga tgagcacctc acgaagggtt ttcttgccct
      841 ggagcacgtg gtggaggttg taccggggcg tgagccccag aatgatatcg atgttggcca
      901 accccatgtc ggcgtcgaaa acgagcaccc ttttaccgag ccttgccaag ctgtaggcaa
      961 gattggccga gacgtgcgtc ttcccgacgc cccctttccc gctggtaacc gccagaacgc
     1021 gtataccgct gtttcgttgt ttctcgacag ctttatattc tgaaggggct acccccgtca
     1081 tttccgcctt taccttcatt ttcctgagtt gcgcggcctg gtccactttt gcctctcctt
     1141 ctcaattcag tctgttccgc acgataagcc tggcaatctt gtcgggttcg gcaacctcga
     1201 tatcctgggg cacgttctgc ccgttggtca gataggaaac cggtttgccg acctggtcca
     1261 cgatgtcgta aatcgggccg aagcgcgtgc attcatctat cttagtaaag atgatgctgc
     1321 cgtaacccat gatcccgaat ctctctgcgg cgtcgagcat atgctcacgg cttgccgtcg
     1381 gactcagaag gaggtgcgtc tccacggggg cgtcacgtat tatccccttg agtttgagga
     1441 ggtggtttcc gtccttcctg ctccggccgg gtgtgtcgat caggacggcc tctttatccg
     1501 agaacttctt cagggcgttc cggaaagcta ccccgtcccc ggcaacctct acggatagtc
     1561 ccattatctt cccgtagacc ttcagctgct cgacggcggc gatcctgtac gtgtccgtcg
     1621 tgatgagtcc gacgctccgc ttctccgtca gggcataacg ggccgccagt tttgcgatgg
     1681 tcgtggtctt accgacgccg gtgggaccga caaaggccaa cgtcctttgc ccctgcactt
     1741 ccctcggagg aggctttatg gaggccctga tcagttcttc cacggcggcg agttgctccg
     1801 cttcctcccc aggacctacc ggagtccttt tccggacgag gctcatcaat cttcgcgccc
     1861 gttcgctgga gatgccgttc gagatcaggt ggtagaaaac gcccgaatgg ttctctttct
     1921 ccctgtcgcg gaggtcgaga aggtcaaaaa gggtgttgac agtattcttt aagtccgtca
     1981 gttcccgcct gatagcggcc ccctccctga cctccctgac gaggaccgtc agatcctcga
     2041 tacggccggc aagcctttcg actccatcag gcttgtcgcc ctctctttcg actttccctg
     2101 ccgtcccgcg gccgggggac ggcagagatt ccctgtcgtc ccgggccgcc atgacctcga
     2161 agaggggctg ccgtccaccc gacagccgct tcgtcgacag gatgaccgcg tcctcaccca
     2221 tatccgcctt gatccgggtc agggcgtcct ggatgttttt tgcctcgtac cttttgatct
     2281 gcatttcgtt actccaccat gcctagcgat tttacgttgg tatcccgcgt cacctcgttg
     2341 tgagagatca ccgccagatt cgggaaaaac ctctcgacga tgttcctgaa gtggatacgc
     2401 gtctgcggtg aacacagaac gaccggctga agcccccgtg acgtgaactg ggggatgtat
     2461 ctctgcaggc ccgtaacgac cttttggata aggtcggggt cggcggccac gtaagattca
     2521 tactcggtat gctgtaccgc acggctgatc gcttcttcga gggccggggc gagcgtaacc
     2581 actgttatgt ctccctttgc gtccctgtac tgcttcgtga tcgtccgagc aagggcctgc
     2641 ctcacgtacc ccgtcaggat gtccacgttc ttcgtcaagt tcacataatc ggcgagcgtt
     2701 tccagtatgg ttaggaggtc gcggatcggg accctttccc ggagaaggcg ctgcaggacc
     2761 ttctggatca agcccagggg caggacattc ggcacgagtt cttccaccac cttcgggtgg
     2821 ttcgcggcca ggctgtccag gagcgacctg acctcttccc tgcgcagaag ttcatcggcg
     2881 tgggatttga tcacctccgt cagatgcgtc gtcacgacag tggcggggtc cacgacgacg
     2941 tagccctttg cctgggcgcc gtccttttcc ttttcgccga cccagacggc gggcaggccg
     3001 aaggccggct ccttcgtttc aatccccttc agtttaagcc ccctgtcgtc agctgcgata
     3061 accagataat g
//
LOCUS       DAOTMX010000208         3071 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_24973_length_3071_cov_3.344828, whole genome shotgun sequence.
ACCESSION   DAOTMX010000208 DAOTMX010000000
VERSION     DAOTMX010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24973_length_3071_cov_3.344828"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1201
                     /gene="feoB"
                     /locus_tag="P5573_06455"
     CDS             <1..1201
                     /gene="feoB"
                     /locus_tag="P5573_06455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HRT27735.1"
                     /translation="SRPRLGQVLITDRIDHVLTRPVFGIPILLCVMAAVLAMTYQIGF
                     PMQKALESAVAHLARATDPVLAHAPAWVKGLVIDGVIAGAGSVLTFLPILLIFFAVMA
                     FLEDVGYMARAAFVMDRFMHVVGLHGKSFLPLCLGLGCNVPAILGARIVESRKERLLT
                     MFLAPFVPCTARLAVLTFVTAALFAERAAAVSWALISLNVVVLGIVGMATTRLFLKKE
                     EPTPFIMELPLYHRPDPRTIAMVIWTRTVAFVKRAGTVILTVSVAVWFLSYLPGGQVE
                     GSFLARFGRLVEPIGAPLGLDWKMIVALLTGVVAKENAVATLGVLYGVGEEGLVHMLP
                     NVVTHASAVAFLVVLMLFIPCAATVAAMRREMGNRKWFLSSFATMLVISYLGGLIAYR
                     FTLWVGL"
     gene            1444..1896
                     /locus_tag="P5573_06460"
     CDS             1444..1896
                     /locus_tag="P5573_06460"
                     /inference="COORDINATES: protein motif:HMM:NF015438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HRT27736.1"
                     /translation="MGQKTSKGKVLSVNVSDRKGEKKHGVDRCMLVEGFGLTGDAHGG
                     FQHRQVSLLARESIDKIKAHGIDVNFGDFAENLTTEGVELFTLPIGTKIRVGGHVLLR
                     VTQIGKTCHDRCAIFRQVGDCVMPREGIFAEVLTGGEVLVGDTIDVLI"
     gene            complement(1963..>3071)
                     /locus_tag="P5573_06465"
     CDS             complement(1963..>3071)
                     /locus_tag="P5573_06465"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: protein motif:HMM:NF006696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HRT27737.1"
                     /translation="SREVVDYLSGFRFTGDVQAMPEGTVFFPQEPVIRVVAPLPQAQL
                     LETRIMNLIHGQVVMASKAARCVLAAGGKAGLIDFGVRRSHGAEAGIFAARSSNIAGF
                     EGTSTVLAEPLYGIPVFGTMAHSFIEAFGSELEAFIDFARANPRATTLLIDTYDTIRG
                     AARAVEAARILEKEGIKVNGVRLDSGDVEALSKEVRKILDEGGLGSARIFVSGHMDET
                     SIRDLLSRGAPIDWFGVGTKLDTSADAPYLDCAYKLMEYGGRPRFKNSEGKETWPGRK
                     QVYRSFRDGTMAEDLVTLEDEDDKGIPLLEPVMKGGRQSAPLPGLEQIRSRAKEQLAA
                     LPGHLRALESSPRYPVRISPLLRKLRKEMEEELG"
BASE COUNT          563 a          967 c          898 g          643 t
ORIGIN      
        1 ttcccggccg agactcgggc aggtcctcat caccgaccgt atcgaccacg tcctgacccg
       61 ccctgtcttc ggtataccga tcctcctctg tgtcatggcc gccgtcctcg ccatgactta
      121 ccagatcggc ttcccaatgc aaaaggccct ggaatcggcg gtcgctcatc ttgcccgggc
      181 gacggacccc gtccttgccc atgcgcctgc ctgggtgaag ggcctcgtca tcgacggggt
      241 aatcgctgga gcaggatctg tcctcacctt cctgcccatc ctgctcatct tcttcgccgt
      301 gatggccttc ctggaagacg tgggctacat ggccagggcc gctttcgtca tggaccgctt
      361 catgcacgtc gtgggactcc acggaaagag ttttctgccc ctgtgtctcg gcttgggatg
      421 caacgtcccg gcgatcctcg gcgcgcggat cgtggaatcc cgaaaagaga ggctcctcac
      481 aatgttcctt gcccccttcg tcccctgcac ggcccggctc gcggtcctta ccttcgtgac
      541 cgccgcgctt ttcgccgaaa gggccgcggc ggtgtcttgg gcgctcatct cgctcaatgt
      601 cgtcgtcctc ggcatcgtgg gcatggcgac cacaaggctt tttctcaaaa aagaagaacc
      661 cacgcccttc atcatggagc tcccgctgta ccaccgcccc gacccgagaa ccatcgccat
      721 ggtaatatgg acaaggacgg tcgccttcgt gaaaagggcg ggaacggtga tcctgacggt
      781 gtccgtcgcc gtctggttcc tgtcttacct tcccggcggt caggtggagg gaagttttct
      841 cgcccggttc ggccgtctcg tagagcctat cggcgcgccc ctgggcttag actggaagat
      901 gatcgttgcg ctcttaaccg gtgtcgtggc caaggaaaac gccgtcgcca ccctcggcgt
      961 tctgtacggc gtgggcgagg aagggctcgt gcacatgctt ccgaatgtag tgacccacgc
     1021 ctcggccgtt gcctttctcg tcgtcctcat gctcttcatt ccctgcgccg ccacggtggc
     1081 ggcaatgcgg cgcgagatgg gaaaccgaaa atggtttctg tcttcctttg cgacgatgct
     1141 cgtcatctct tacctcggcg gtctcatcgc ctaccgtttc accctgtggg tgggtcttta
     1201 gtcttatcat gcaccttaaa aaaaggaggg cctttctcgt caaggctacg ccctcgatca
     1261 cagcatcgct gcttttcgtc ccggcgctct tgaaaacacg ctcatcgcca ttttcaggtt
     1321 tattttgccc gaaaaagata ttaagctctg tggcgggaat ggatacggcg ctcgacatgg
     1381 agatgatcgg ggacccggga cgcgccgcaa aaatcgaagg cgagaagttg agaagataag
     1441 aagatgggac aaaagacatc gaaggggaag gttctttctg tcaacgtctc cgacaggaag
     1501 ggagagaaaa agcacggtgt cgacaggtgc atgctggtcg agggtttcgg gctcaccggc
     1561 gacgcccatg ggggatttca gcaccggcag gtctccctcc tcgcaaggga gagcatcgac
     1621 aagatcaagg cccacggcat tgacgtcaac ttcggtgact tcgcagaaaa cctcacgacg
     1681 gaaggggtcg agcttttcac actccccatt gggacaaaaa tacgcgtcgg cggacacgtc
     1741 cttctgagag tcacccagat cgggaaaaca tgccacgacc ggtgcgcgat cttccggcag
     1801 gtgggtgatt gcgtcatgcc gcgggaaggt attttcgccg aagtccttac aggcggcgaa
     1861 gtcctggtcg gcgatacgat cgatgtcctc atataacagt cggcgacatt gcgtggtagc
     1921 gtcgagccgt caagacaccg gccgacccga cagggcctct cctcagccca actcctcttc
     1981 catctcctta cggagtttcc ggagcaacgg cgaaatacgg acagggtaac ggggggacga
     2041 ttcgagggcc cttaggtgcc ccggcagggc tgccaactgt tcctttgccc gggatcggat
     2101 ttgctccagg ccgggcaacg gggcgctttg tctgccgccc ttcatgaccg gttcgagcag
     2161 ggggatccct ttatcatctt cgtcttcgag ggtgaccagg tcttccgcca tcgtcccgtc
     2221 cctgaaagag cggtagacct gcttccttcc cggccatgtc tccttgcctt cggaattctt
     2281 gaaccgcggc cttcccccgt actccatcag cttgtaggcg cagtcgagat aaggcgcatc
     2341 ggctgaggtg tcgagcttcg tcccgacgcc gaaccaatcg atgggcgccc cccgggagag
     2401 gaggtcccgg atggatgtct cgtccatgtg cccgctcacg aatatccggg cgctcccgag
     2461 gcccccctca tcgagtattt ttcgcacctc cttcgacagg gcttctacat cgccgctgtc
     2521 gagccggacc ccgttgacct tgatcccctc tttctcaagg atccgtgccg cttcgacggc
     2581 cctcgctgct cccctgatgg tgtcgtaggt gtcgatgagg agggttgtcg ccctggggtt
     2641 ggcccgcgca aaatcgatga acgcctccag ctcgcttcca aacgcctcga tgaaggagtg
     2701 ggccatcgtc ccgaagaccg ggatgccgta aaggggctca gcgagcacgg tggaagtccc
     2761 ctcaaacccg gcgatgttac tcgagcgggc ggcaaagata ccggcctcgg caccatggga
     2821 acggcggacc ccgaagtcga tcagtccggc ctttcctccc gccgcgagga cacagcgcgc
     2881 ggccttgctc gccatgacga cctgaccgtg gatgaggttc atgatccggg tctctaagag
     2941 ttgcgcctgg ggtaacggcg ccacaacacg gatgaccggt tcctggggga agaagacggt
     3001 cccctcgggc atcgcctgga cgtcgccggt gaaacggaac ccgctgagat aatccacaac
     3061 ctcccgggag a
//
LOCUS       DAOTMX010000209         3067 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_25013_length_3067_cov_3.492696, whole genome shotgun sequence.
ACCESSION   DAOTMX010000209 DAOTMX010000000
VERSION     DAOTMX010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25013_length_3067_cov_3.492696"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="P5573_06470"
     CDS             complement(<1..353)
                     /locus_tag="P5573_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27738.1"
                     /translation="MMKRRLKKSRRILQLLIFFLLCSGCGTPSDPPSIGRACEREPQG
                     ERPAATASNQCRIRYAKGFDVSYHDGCKIVTVFEPGQAPGSKGTATFVLVPRGTRRSL
                     PVEPARGAVIEIPVRR"
     gene            complement(427..2463)
                     /locus_tag="P5573_06475"
     CDS             complement(427..2463)
                     /locus_tag="P5573_06475"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRT27739.1"
                     /translation="MEKKDSFTTKELTFYLIVCLFTCLCAPPGAPAEEVSADKAEGKH
                     VSKMEEVVVTATRVEEPKKDVSSSVQIITAEDIKSSTAKDAGDLIAESGVGHVHKYSG
                     ALTSMIEIRGLATDSMSDPLNSRVLVLINGNNAGTVNLAKIPVDDIERIEIVKGPASV
                     LYGSSAMGGVINIITKQGRDGFHGSVGGEGGSFGYWKAGAEAGGKEDRFDYYVTASRS
                     GQGDYRTPDFGKIENSGYKDETVSTRFGYKLFNDHRVSVGYQHWKGWDIGTPGPTYAS
                     DPDDYKDIKRDAVDVGYQTPSFQAKYYCVWDRDEDHSTPFWGLGPGNSWINRTDRDSQ
                     GANLQKTFSVGGQRVVVGGQWDRVETTSWRNEGAPYYPDSRYDSYGVFSEGRLSLFDK
                     KLLLNAGVRYDYFQDEILATPGIADLKPRKENLDHVTARGGAVYKLTDALSLKGNVGT
                     AFRAPAPMELASDYYSWGTHYLGNPDLEPEESITYDAGVDYSRGPFKGDLTFFHTDFR
                     KKIVRYYDTSLAASTYKNADSATLQGLELDAAYDIGHALGIGVILEPFANVTYRTKYL
                     QEVNGIESRLTYTARWTAAFGMRVGQKKWDGRLIVNYRGQEDVTDFNFSSPTYGQTVE
                     KGDFTVVSLKGSYRPIKNLELTASIENLFDRQYEYVLGYPMQGIIFVGGAKILF"
     regulatory      complement(2498..2697)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(2753..>3067)
                     /locus_tag="P5573_06480"
     CDS             complement(2753..>3067)
                     /locus_tag="P5573_06480"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRT27740.1"
                     /translation="QGLSVNLPHAVTARNEVRQTATLTLTKEGRLYFNKEPVSSPEEA
                     ASRAARWKETVTEAVAVINADRSVEHGSVVALMDAMRRAGVTRMAIAADPNDISETGE
                     RR"
BASE COUNT          535 a          895 c          895 g          742 t
ORIGIN      
        1 cgccggacgg gaatctcgat gaccgcgccc cttgccggct cgaccggcag ggaccggcgt
       61 gttcccctgg gaacgagcac gaacgtcgct gtcccttttg aaccaggagc ctgacccggt
      121 tcgaagaccg tgacgatctt gcagccgtcg tgatacgaca cgtcaaaccc cttggcatag
      181 cggatgcggc actgatttga cgccgttgcc gcggggcgtt cgccctgggg ttctctttcg
      241 caggcccgtc ctatagaggg ggggtcggat ggagtcccgc agccggagca gagaaggaaa
      301 aaaatgagga gttggaggat ccgcctgctt tttttcaacc gtcgcttcat catgaccttg
      361 ttgcccctgt tgttcggcgg ccccgcgggt cctgtatcga agcccgtagg ggccgcctct
      421 ccgccttcag aaaagtatct tggccccgcc gacgaaaata ataccctgca tggggtagcc
      481 caggacgtac tcgtactgcc tgtcgaagag attctcgatc gaggcggtca attccaggtt
      541 cttgataggc cggtaagacc ctttcaggct taccacggtg aagtcacctt tttctacggt
      601 ctgaccgtag gtcggcgaag aaaaattgaa gtcggtgacg tcttcctgcc cccggtaatt
      661 gacgatgagc cgtccgtccc attttttctg tccgacccgc attccaaagg cggcggtcca
      721 cctggccgta taggtcagcc gactctcgat gccgttgact tcctggagat attttgttcg
      781 gtaggtgaca ttggcaaagg gttcgaggat cacccctatc ccaagcgcat gaccgatgtc
      841 gtaggcggcg tccagctcca gcccctgcag ggtcgcactg tcggcgtttt tataagtgct
      901 tgctgcgagc gaggtgtcat agtagcggac gatctttttt ctgaagtccg tatggaagaa
      961 tgtgaggtcg cccttgaaag gtcccctgga gtagtcgacc ccggcatcgt acgtgatgct
     1021 ctcctccggc tcaaggtcgg ggttgccgag gtagtgcgtt ccccatgagt agtagtcgga
     1081 tgcaagttcc atgggcgccg gggcccggaa ggcggtcccg acgttgccct tgaggctcag
     1141 ggcgtcggtc aatttataga ctgcgccgcc cctcgcggtg acgtggtcga ggttttcttt
     1201 tcggggcttg agatcggcaa ttcccggtgt ggctagtatc tcgtcctgga agtaatcgta
     1261 gcgcacgccg gcgttcagca ataacttctt atcgaaaagg ctgagacgtc cctcactgaa
     1321 gacaccgtag ctgtcgtacc gggaatcagg gtagtagggc gcgccctcgt tcctccaact
     1381 tgtcgtctcg accctgtccc actggccgcc cacgaccacc ctttgaccgc cgacggagaa
     1441 ggtcttctga aggtttgcgc cctgtgaatc cctgtcggtt ctattgatcc atgaattacc
     1501 gggacccaat ccccagaagg gggtgctgtg gtcttcgtcc ctgtcccaga cgcagtagta
     1561 ctttgcctgg aacgaggggg tttgatagcc gacgtcgacg gcgtcccgct ttatgtcttt
     1621 gtagtcgtcg gggtcggagg cgtatgtcgg tccgggagtc ccgatatccc agcctttcca
     1681 gtgctggtaa ccgacggaga cgcggtggtc gttgaacagc ttgtagccga aacgggttga
     1741 aacggtttcg tctttatagc ctgagttctc gatcttgccg aagtccgggg tccgatagtc
     1801 gccctgtccc gaccggcttg cggtgacata atagtcaaag cggtcttctt ttccgcccgc
     1861 ttcggcgccg gccttccagt aaccgaacga acctccttca ccccccacgg aaccatgaaa
     1921 cccgtcccgc ccctgtttgg tgatgatgtt gatgacgccg cccatggcgg atgaaccgta
     1981 gaggacggag gccggcccct tgactatctc gattcgttcg atgtcgtcga cgggtatctt
     2041 tgcgagattg accgtgcccg cattgttgcc gttgatgaga acgaggaccc ggctgttcag
     2101 ggggtcgctc atggaatcgg tcgccagtcc tcttatttct atcatgctgg tgagggcgcc
     2161 gctgtactta tggacgtggc cgacgccgga ttcggcaatg aggtcgcccg cgtcttttgc
     2221 tgtggaactc ttgatgtcct cggcggtgat gatctggacc gacgaggaga cgtccttttt
     2281 cggctcctcg accctggtag cggttacgac cacctcttcc atcttcgata cgtgttttcc
     2341 ttccgcctta tcggcagaca cctcttcggc aggcgcgccg ggcggcgcgc aaaggcatgt
     2401 aaaaaggcat acgataagat agaaagtcag ttcctttgtc gtgaatgagt cctttttttc
     2461 caattttcct ttcctccctc gggaaataat cattgagaat tatgattgtg aaagagagag
     2521 gtcttccggc ttgagggcga tcgctgtttc ggatgcctac ttgcccgcct tcccatccgg
     2581 ctgttgcgcc ggacagtggc tcgtctgatg tttcgggctt tcgttccctt cacggctgcg
     2641 gggcagcggg agattcacac tcctcttccc tgacttctct tcacggtttc agtacggcac
     2701 gcccggcatg cgctgccgcg gcaatacgac aacgtctctg aagcctggtt gctcacctcc
     2761 tttcaccagt ttccgaaata tcgttggggt ctgcggcgat ggccatcctc gtcacgccgg
     2821 cccttctcat cgcatccatg agcgcgacta cgctgccgtg ttccacagac cggtcggcat
     2881 tgatgacggc gacggcctcc gtcacggtct ccttccatcg ggcagcgcgc gaagccgcct
     2941 cttccgggga ggaaaccggt tctttgttga aataaagcct tccctccttc gtgagcgtca
     3001 gggtcgccgt ttggcgaacc tcattgcgcg ccgtgacggc atgtgggagg tttacggaaa
     3061 gcccctg
//
LOCUS       DAOTMX010000210         3066 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_25025_length_3066_cov_3.544670, whole genome shotgun sequence.
ACCESSION   DAOTMX010000210 DAOTMX010000000
VERSION     DAOTMX010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25025_length_3066_cov_3.544670"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /locus_tag="P5573_06485"
     CDS             complement(<1..841)
                     /locus_tag="P5573_06485"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRT27741.1"
                     /translation="MLTENEKIETLIRLSVELNQLQDFDILMEHILAEARNFVNADAG
                     SIYIRDGDRLIFSYTQNETLQKRMPPGGKLVYSTFSIPVDETSIAGFSAATGKTLNIS
                     DVWAIGNSRPYRFNKDFDEKACYRTRSVLTIPLKTLQGNVIGILQIINARDGNEKVIP
                     FSTEDEKMMLHFASTATVALERAQLVRNIILRTIKMTEMRDPKETGAHVNRVASCATE
                     IYEKWARLKGIPERQIDLNRDLLRMAAMLHDAGKVAIPDALLKKPGKLTDEEFKVMQS
                     HTIQ"
     gene            complement(857..2167)
                     /gene="eno"
                     /locus_tag="P5573_06490"
     CDS             complement(857..2167)
                     /gene="eno"
                     /locus_tag="P5573_06490"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HRT27742.1"
                     /translation="MSEIINVQAREILDSRGNPTVEVEVELISGIIGRASVPSGASTG
                     EHEMLELRDGDSKRYRGLGVQKAIQNIHEKIRPELIGMDCFMQRQIDYMMVELDGTEN
                     RSNLGANAILGVSLACARASAEWFGMSLYRYIGGVAAKDVPVPMVNIVNGGQHADNNV
                     DLQEFMVMPAGAKNFKEALRMASEVFHSLRAVLKKKGYNTAVGDEGGFAPNLRSNEEA
                     LVVIMEAIGKAGYEAGKDIFIALDSAASSFFEKGKYILAAEKKPKKTPEEMVKFYSDI
                     VKNYPIISIEDGLAEDDWEGWKLLTDELGAKVQIVGDDLFVTNKKRLEMGIARGIANS
                     ILIKVNQIGTLTETLETIQRAKEAGYTTVVSHRSGETEDTSIADIAVAANCGQIKSGS
                     VSRSERLAKYNQLMRIEEELGDMAVYPGMSAFYSIRKTGKKPGE"
     gene            2453..>3066
                     /locus_tag="P5573_06495"
     CDS             2453..>3066
                     /locus_tag="P5573_06495"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRT27743.1"
                     /translation="MDYNMLETGDRVIVGISGGIDSLALLDLLSSPMVHAPSDFSLLA
                     VNIDMGFDRTYGGFAALECFLRENGYDYVMEKSDIGPLSHSDYNRKNPCYLCSRLRRR
                     RIFEIAAERGCNKIALAHHKDDIVETLLINIFYGREISTMMPRQSIFGGKLQIIRPFA
                     YIREELVKKYGRERGFPVVENPCPTSKVSKRTVVKKLLKELEQE"
BASE COUNT          607 a          862 c          829 g          768 t
ORIGIN      
        1 cttgaatcgt atgagactgc atcaccttga actcttcatc ggtgagtttt cccggtttct
       61 tgagtaaggc gtccgggatg gccacctttc ccgcatcgtg gagcatggca gccatgcgca
      121 acaggtcccg gttcaaatcg atctgtcgct cgggaatccc tttgagtctc gcccacttct
      181 cgtaaatctc cgttgcgcag ctcgcgaccc ggtttacatg ggcccccgtc tcttttgggt
      241 cccgcatctc ggtcatcttg atcgtccgca agatgatgtt cctaacgagt tgcgcccgtt
      301 cgagcgcaac ggtagccgtg ctcgcgaagt gaagcatcat cttttcgtct tccgtcgaga
      361 aagggatgac cttttcgttg ccgtcccgcg cgttgatgat ctgaaggatg ccgatgacgt
      421 tcccttgtaa ggtcttgagc gggatcgtca gcacggaccg tgtcctataa caggccttct
      481 cgtcgaagtc cttgttgaat cggtaaggac gactgttgcc gatagcccac acgtccgaga
      541 tgttcagcgt ctttccggtg gcggcggaga agccggcgat gcttgtctcg tccacgggga
      601 tcgagaaggt ggagtagacg agcttgccgc ccggcggcat tcgtttctgt aaggtttcgt
      661 tctgggtata actgaagatc aggcggtctc cgtcgcggat gtagatggaa cccgcatcgg
      721 cgttgacgaa gttcctggcc tccgccagga tatgttccat gaggatgtcg aagtcttgca
      781 actggttcag ttcgacactg agcctgatga gtgtttctat tttttcgttt tccgtcagca
      841 tgccgctccc gtgaggttat tccccgggtt ttttgccggt cttacggata ctgtaaaaag
      901 cggacatccc cggatatacg gccatgtcgc cgagttcttc ctctatcctc ataagctgat
      961 tgtactttgc aagccgctca ctccgcgaga cggacccgct ctttatctgg ccgcaattcg
     1021 ccgctacggc tatatcggcg atggacgtgt cctccgtctc gcccgaccgg tgagagacga
     1081 ccgtcgtgta gcccgcctct tttgcgcgct gtatcgtctc cagcgtctca gtgagcgtgc
     1141 cgatctggtt taccttgatc aggatcgagt tggcgatgcc tcttgcgatg cccatctcca
     1201 gtcgtttctt gttggttacg aagaggtcgt caccgactat ctggaccttt gctccgagct
     1261 cgtcggtcaa gagtttccaa ccctcccagt cgtcctccgc caggccgtcc tcgatcgaga
     1321 tgatcgggta gtttttcacg atgtcgctgt agaacttgac catctcctcg ggtgtcttct
     1381 ttggcttttt ttccgcagcg aggatgtatt tccccttttc gaagaaagag ctcgctgccg
     1441 agtcaagggc gatgaaaatg tccttacccg cttcgtagcc ggccttcccg atggcttcca
     1501 ttatgacgac gagggcctct tcgttggatc tcaggttcgg cgcgaaaccc ccttcatccc
     1561 cgaccgccgt gttgtaacct tttttcttca ggaccgccct caggctgtga aacacctcgc
     1621 tggccatccg gagggcctct ttgaaatttt tcgcgccggc aggcatgacc atgaattcct
     1681 ggaggtcgac gttgttgtct gcatgctgtc cgccgttcac aatgttgacc atggggaccg
     1741 ggacatcctt ggcggccaca ccgccgatgt aacggtaaag ggacatgccg aaccactctg
     1801 cagaggccct ggcgcaggcg agggagacgc cgaggatggc gttggcgccc aggttgctcc
     1861 tgttctccgt cccgtcgagt tcaaccatca tgtagtcgat ctgccgctgc ataaagcaat
     1921 ccataccgat gagctcaggc cgtatcttct cgtggatgtt ctggatcgcc ttctggacgc
     1981 cgagacccct gtatctcttc gagtctccgt ctctcaattc gagcatctcg tgttcccccg
     2041 tcgaagcgcc cgaagggact gaagccctgc cgatgatacc cgatatcaac tcgacttcga
     2101 cctcaaccgt gggatttcct cttgaatcca agatttctcg cgcttggaca ttgatgatct
     2161 cactcatatg gcctccttga ggggtgtgta ttgaaagatg ttatggtatt aacgcaacaa
     2221 ggcttttttt tcaaggtgaa tgtctaacgt gaagtagctt gccaagacca tgccattcga
     2281 gtataaaaac gggcaccagg aagctaagag gcatcgtttc agtatggttc aacaggtgtt
     2341 gcgcaccaat tcttctaaac cggccgtact cgaaaaagtc aggcccgcag tgccacgcga
     2401 aaagggcacg aagcttttcc ttcatctcaa gaaatggatg gagaaggcag tgatggacta
     2461 taacatgctg gaaacggggg accgggtaat cgtcggcatc tcgggcggca tcgacagcct
     2521 cgcactcctc gacctcctca gctcccccat ggttcacgcg ccgtccgatt tttccctcct
     2581 cgccgtcaat atagatatgg ggttcgacag gacttacgga gggtttgccg ccctggagtg
     2641 ttttcttcgg gaaaacgggt atgactatgt gatggagaag agcgatatag gccctctgtc
     2701 ccacagcgat tacaaccgca agaacccctg ttatctctgc tcgcgtctca ggcgccggag
     2761 gattttcgag atcgcggcgg aacgggggtg caataaaatc gcccttgccc accacaagga
     2821 cgacatcgtc gagacgcttt tgatcaacat cttctacggc cgggaaatca gcacgatgat
     2881 gcccaggcag tccatcttcg gcggaaagct ccagatcatc cggccctttg catacatacg
     2941 ggaggaattg gtgaaaaagt acggcaggga gcgggggttt ccggtggtcg agaacccctg
     3001 tcccacgagc aaggtttcga aaaggactgt cgtcaagaaa ctgctgaagg aactcgagca
     3061 ggaaaa
//
LOCUS       DAOTMX010000211         3054 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_25151_length_3054_cov_4.193731, whole genome shotgun sequence.
ACCESSION   DAOTMX010000211 DAOTMX010000000
VERSION     DAOTMX010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25151_length_3054_cov_4.193731"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..990)
                     /locus_tag="P5573_06500"
     CDS             complement(<1..990)
                     /locus_tag="P5573_06500"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HRT27744.1"
                     /translation="MPRIRGKTKTAEILTLEDAVKKHMKNGIQIAFSGFTGMNRNPFA
                     FAWEIVRQGFKDLHSIDRHGSCCTWLLNAVNALKIQETDWLGWGEMAGKIDLNMERNY
                     KAGKMIIEDYAHGAMAFRFLAGAVGAPFMPYYAPLGSDLYNPKYDALGRAGLRDGKNP
                     KIAKKKFLQMEDPFWGDGEVVLLPAARPNLAVLHVLQVGDKGTARWRGVGTIDKEIAF
                     AADEVVVVAEEIVPEGELRKYPETNQVAYFTVDTIVECPWGAFPSAVPFCYDYDVPFL
                     RSMDAASRNETDLKKWLDEWVFGPKNWEDFIVKLGTKRLIDLKADSVTGYSTRL"
     gene            complement(1348..1722)
                     /locus_tag="P5573_06505"
     CDS             complement(1348..1722)
                     /locus_tag="P5573_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27745.1"
                     /translation="MLSKEEVRERALYCYSVFLQLSWLYSNNFIAPTQYIDSLKESSL
                     GLGEDEFITLTIKEALMEEREDGGLRTLMALYEGFAGAFCEVLEISLDDIHAELSPEY
                     CRQLASEVGVDLNRIEKVLNSP"
     gene            1994..2977
                     /locus_tag="P5573_06510"
     CDS             1994..2977
                     /locus_tag="P5573_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRT27746.1"
                     /translation="MAKVLQVKNLRTCFDTESGTVRAVDGVDLSLEEGETLGIAGESG
                     CGKTVLALSIMRLIPSPPGRVASGQVMLDGVDITRLSDEEMRKIRGRGISMIFQEPMT
                     SLNPVIPVGEQIAEAVRLHLGFSRKDAACKAVEMLKTVGIPSPSERVKDYPHQMSGGM
                     RQRVMIAIALSCRPRLMLADEPTTALDVTIQAQILDLINRLKEEAKTSVVLITHDLGV
                     IAEAAQKVAVMYAGLVVEYSSVRELYYEPRHPYTVGLLESRPGPREKDARDRHLKVIP
                     GTVPNLYELPPGCPFHNRCNVVMDRCKMERPEYTEISEGHYVRCWRINPWT"
BASE COUNT          726 a          788 c          797 g          743 t
ORIGIN      
        1 caggcgggtg ctgtagccgg tcacactgtc ggccttgagg tcgatgagcc gtttggtacc
       61 cagtttgacg atgaagtctt cccagttttt cgggccgaag acccactcat cgagccattt
      121 cttgaggtcc gtttcgtttc ttgacgcagc gtccatgctc ctcaggaagg ggacgtcgta
      181 gtcgtaacag aaaggtactg cgctcgggaa ggcaccccag gggcattcga ctatcgtatc
      241 tacggtgaaa tatgcaacct ggttggtttc cgggtacttc ctcaattctc cttcgggaac
      301 gatttcttcc gcgacaacga ctacctcgtc ggcggcaaag gcgatttcct tatcgatcgt
      361 cccaacgccc ctccatcttg ctgttccctt gtcaccaacc tgaagtacgt ggagcacggc
      421 gagattgggt cttgctgcag gcaggagaac tacctctccg tcaccccaga aaggatcctc
      481 catctgtagg aattttttct tggcgatctt agggttcttg ccgtcacgca gcccggccct
      541 tcccagggca tcgtatttcg ggttgtaaag gtctgatcca agaggggcgt agtaaggcat
      601 aaacggagcc cccaccgctc ccgcgaggaa tctgaaggcc atggcaccgt gggcgtaatc
      661 ctcgatgatc atcttgccgg ccttgtaatt acgctccatg ttgaggtcaa tcttgccggc
      721 catctcaccc cagccgagcc agtccgtctc ctgaatcttg agtgcgttta cggcattcag
      781 gagccaggtg cagcagcttc catgcctgtc tatgctatgg agatccttga agccttggcg
      841 gacaatctcc caggcaaatg caaagggatt cctgttcata ccggtaaaac cgctaaacgc
      901 gatctgtatc ccgtttttca tgtgtttttt gacggcgtct tcgagcgtta aaatctctgc
      961 ggtttttgtc tttccacgaa ttctaggcat tgtttcctcc tttttaacaa agatattcta
     1021 ccccctccaa caactttcct tcccggaaga ataataatct tccaccagca tattgcagaa
     1081 cgagatggtt cacccccttt cgtcatataa gttgaggcaa aaaaagactc cttggaaaaa
     1141 tcagagaata gtttgattcg gaatagcgac tgatgagcgc aaaggacatc cgacgaaccc
     1201 ggtctaactt gattatctcg ctgtcaacaa tataactcat caatactatt gagtaaacca
     1261 gcgaactaaa gatttaaaat cgactgactc gacatgacat cgggacgact ggaatgttaa
     1321 aatgtatagg atattaacct ttaagtgtca aggagaattt aggacttttt cgattctgtt
     1381 gaggtctacg ccgacctcag aggcgagttg tcggcagtat tcgggagaaa gttccgcatg
     1441 tatatcgtcg aggctgatct cgagaacctc acagaacgcc cctgcaaagc cttcatacag
     1501 cgccataaga gtccttaggc cgccgtcttc cctctcttcc ataagggctt ccttgatggt
     1561 aagggttatg aattcatctt ctcccagtcc gagtgacgat tccttaagag aatcaatgta
     1621 ttgagtcggt gcgatgaagt tgttgctgta gagccagctc agttggagaa aaacactgta
     1681 gcaatacaag gcccgttcgc gtacctcttc tttggacaac attggatgtt ccttctgacg
     1741 caatcgatcg gtgaatgaga cgctgaaatt tctatatgag caaggctcca gctgtcaaga
     1801 aaaagcgata caaaacggtc gagagaaata acccctcaaa cctgttcgaa ttgacttatc
     1861 cctcctttat gctatcgtct caatcatttt tggggaaggc gacgggcttc gcggtgagaa
     1921 aacccaagag ttctcaagcg acggtagtcg tggccgggcc ttttgaagag attctcgaga
     1981 gcgggaaaac tgtatggcga aggtccttca ggtaaagaat ctgcgtacct gttttgatac
     2041 cgaaagcggc acggttcgtg cggtggacgg tgtcgacctg tccctggaag agggagagac
     2101 gctcggcatc gccggcgagt ccgggtgcgg caaaacagtt cttgcccttt caataatgag
     2161 gctcatccca tcgcctcccg gccgggtcgc ctccggtcag gttatgttgg atggcgtgga
     2221 tatcacacgt ctcagcgacg aggaaatgcg gaagatcagg ggtcgcggga tctcgatgat
     2281 attccaggaa ccgatgacct ctttgaaccc cgtgattccc gtcggtgaac agatcgccga
     2341 ggccgtccga cttcatctgg gcttttcgag aaaagatgcc gcttgcaagg ccgtagaaat
     2401 gctcaagacg gtaggaattc catcgccttc agaaagggtg aaggactatc cccaccagat
     2461 gagcggcggg atgcggcaga gagtgatgat cgccatcgcc ctgtcctgtc ggcctaggtt
     2521 gatgcttgca gacgaaccga ctaccgcgct cgacgtcact atccaggcgc agatcctgga
     2581 cctcatcaac cgtctgaagg aagaggcgaa gacatcggtc gtcctgatta cccacgacct
     2641 cggagtcatt gccgaggcgg cccagaaggt agccgtcatg tacgccggtc tggtggtgga
     2701 gtacagttcc gtcagggaac tgtattacga gccacggcat ccctataccg taggactcct
     2761 tgagtcccgc cctggaccga gggaaaagga cgctcgggat cgtcatctga aggttatacc
     2821 ggggacggtc cctaacctct acgaactacc cccgggttgt cccttccaca atcggtgcaa
     2881 cgttgtgatg gaccgttgca agatggaacg gcccgagtac accgaaatat cggaaggtca
     2941 ctatgtacga tgttggagaa taaatccttg gacatagagg catatgaaga ttcagggtcg
     3001 gcacggacga ttctttcagt tgagtgtctg aagaagtact ttgagtcgag aagc
//
LOCUS       DAOTMX010000212         3046 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_25241_length_3046_cov_3.585757, whole genome shotgun sequence.
ACCESSION   DAOTMX010000212 DAOTMX010000000
VERSION     DAOTMX010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25241_length_3046_cov_3.585757"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="P5573_06515"
     CDS             <1..701
                     /locus_tag="P5573_06515"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRT27747.1"
                     /translation="RTVRERAQALIDIAHPDDREWLVKQAKEARLLYPDQVFFPESGR
                     LYPEKIATTHTFKDGLTVRFRAIKPSDEDEMRRLFYRFSDRAVYYRYFSPIKTMPHDR
                     MQEYVNVDYRTTMSVVGVVEEAGRERIIAEARYVRAKDRPYADIAFVVDEAYQGKGIA
                     SFMMEMLVRIAKEQGGIEGFVGDIVTDNKAMLKVFEKSPCTARTVLSGGYYEVTMPFS
                     EKKGTETRDAKDQS"
     gene            682..1152
                     /locus_tag="P5573_06520"
     CDS             682..1152
                     /locus_tag="P5573_06520"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRT27748.1"
                     /translation="MRKINPEYVRAVMALANSGSYFRLISMSIRELGRGHCLMEAEID
                     GQKHLQAFGFVHGGVYASLIDTAAFWAAICDLDEDVGITTVDLNVNYLAPIQEGKLVT
                     RGKLIKLGRTLGLGEATVTTETGKLVAHGTSTLMLVPGLGFARGKDLPPKFMKP"
     gene            complement(1172..1591)
                     /locus_tag="P5573_06525"
     CDS             complement(1172..1591)
                     /locus_tag="P5573_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27749.1"
                     /translation="MPPLVFLLMVIACGGKGSGGGGGNPSTPTISNVSFMPSQLPRGT
                     RISNNLYVSFDWTDGGGDLNGGTFTLIYEGKYYTTPLTAAVPPINPGSTSGHVNQLYL
                     SPFTLSSTSGVPRIQVRLTDNAGNASSLVFVTNFYQT"
     gene            complement(1659..2120)
                     /locus_tag="P5573_06530"
     CDS             complement(1659..2120)
                     /locus_tag="P5573_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27750.1"
                     /translation="MGSLLSGLLMAVLVFSSSCPSVFAKELFLLRNARLDEPVGLVQV
                     NALKKKAESSFRPGDRVTAVYLFNPTGTEEAVEFRWLQQVGRTLVQKESYTHRMERQN
                     PGGVYVAYSWVVFDSTFLDRFLGSGFSGYWLVEVYVNDRKEAEKLFFVAPD"
     gene            2340..>3046
                     /locus_tag="P5573_06535"
     CDS             2340..>3046
                     /locus_tag="P5573_06535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRT27751.1"
                     /translation="MDPTRPRKKKMVTKSLFESDYDLSGLLSQFIPAREKTGLGEGIL
                     SDIYRAIVESASDSMYIVDRYCRYLFMNGAHLKRLDLTLEEVVGRTYGEFHSEEQKRE
                     FEDIINEVLTHGLPAKQEHRSGRDSRYFLRTFNLLKSPSGEKTTAAVVVISKDITEWK
                     QAEVALREGEEKYRTILNGIADGYYEVDLQGNLMFVNPSMTSILGYSEEELLGLNNRQ
                     FMDKENARTVFQVFNTV"
BASE COUNT          766 a          845 c          818 g          617 t
ORIGIN      
        1 gccgcacggt gcgtgagcgg gcccaggccc tcatcgacat cgcccacccc gacgacaggg
       61 agtggctcgt gaagcaggca aaagaggcaa ggcttctcta tcccgaccag gtgtttttcc
      121 ccgaatcggg acggctctat cccgagaaaa tcgccacgac acataccttc aaggacggcc
      181 tcaccgtgcg tttccgggca atcaaaccgt ccgacgaaga cgagatgcga aggctgttct
      241 accgcttttc cgatcgagcc gtatattacc gctatttcag cccgatcaag accatgcccc
      301 atgataggat gcaggagtat gtaaacgtag attatcgaac aaccatgtcc gtcgtgggag
      361 tcgtcgaaga ggccgggagg gaaaggatca tcgccgaagc ccggtatgtc cgcgccaaag
      421 accgccccta tgccgacatt gctttcgtcg tcgacgaggc ctaccagggc aaagggatcg
      481 cctccttcat gatggagatg ctggtccgaa tcgccaaaga acagggcggg atagaaggct
      541 tcgtgggcga catcgtgacc gacaacaagg cgatgctgaa ggtcttcgag aaatcgcctt
      601 gcaccgcacg cacagtgctt tccggcggat actacgaagt gaccatgcct ttttcagaaa
      661 agaaaggaac tgagacccgc gatgcgaaag atcaatcctg aatacgtccg cgccgtgatg
      721 gccctggcca acagcggctc ctacttccga ctcatttcga tgagcatccg cgaactgggt
      781 agaggacatt gcctgatgga agcggaaatc gacggacaga agcatttgca ggccttcggt
      841 ttcgtccacg gtggtgtcta cgcctccctc atcgacacag cggccttctg ggcggccatc
      901 tgcgacctcg atgaagacgt gggaataacg acagttgacc tgaacgtcaa ctacttggcg
      961 cctattcagg agggcaagct cgtaaccaga gggaaactga tcaagctcgg gaggaccctc
     1021 ggcctcggcg aggcgacggt taccacggaa acgggaaaac tcgttgccca tggaacgtca
     1081 accctgatgt tggtgccggg gcttggcttc gcgagaggca aagacctgcc gccgaaattc
     1141 atgaaacctt aaaaagttgc attcctttct tttacgtttg ataaaagttc gtcacgaaga
     1201 caagcgacga ggcgttgccg gcattgtcgg tgagccggac ctgaatacgc ggcaccccgg
     1261 aagtggaact gagcgtaaag ggcgaaaggt atagttgatt cacgtggccg ctcgtgctgc
     1321 cgggattgat gggagggacg gccgccgtca gaggtgtcgt gtagtatttg ccctcgtaga
     1381 taagagtaaa agtcccgccg ttcaggtctc ctcccccgtc tgtccaatcg aaggagacgt
     1441 agaggttgtt gcttatccgc gttcctctgg gaagctgact gggcataaaa ctgacgttgc
     1501 ttatcgtcgg cgtggagggg tttccccctc ctccgccgct tcccttgccg ccgcaggcaa
     1561 taaccatcag cagaaaaaca aggggcggca aaaatatttt ccaataaaat tgcatggttt
     1621 ctttgtttta atcgagcaaa aacatcgccc tgccctggtc aatcaggcgc cacgaagaag
     1681 agcttctccg cctctttccg gtcattgaca tagacctcta cgagccagta gccggagaaa
     1741 ccagacccca gaaacctgtc gagaaacgtc gagtcgaaca caacccagga ataggcaaca
     1801 tagaccccac ccgggttctg ccgctccatc cggtgcgtat aactttcttt ctgaaccaag
     1861 gtgcggccca cctgctgcaa ccagcggaat tcgacggcct cctccgtccc cgttgggttg
     1921 aagagataca ccgccgtcac cctgtctccc gggcgaaaag aggactctgc cttttttttg
     1981 agagcattga cctgaacgag gccgaccggt tcatcaagcc tggcgttcct taagaggaaa
     2041 agttccttcg caaaaacaga aggacaggaa gacgaaaaca ccagaactgc catcagcagg
     2101 ccggataata gggaccccat cgcgaagact cctgcaaacg cactgtcggg ctcaccccgc
     2161 agattgcacc agcaaccttc tgaatacaaa atacctgact cgaccggtgt cgataataac
     2221 ggtgtgaaac cacgcctgtc aacatattat gggacttctt tcttatttaa tattgtgttc
     2281 ttctgtaaaa tttgttagac atcttcaaca tacgtgagtt ttttcgaacc gaccatctta
     2341 tggatccgac aagaccccgc aagaagaaga tggtgacgaa aagtctgttt gagtccgact
     2401 acgacctgtc ggggttgctc agccaattca ttccggcccg agaaaaaacc ggcttgggcg
     2461 aaggaatcct ctccgacatc tatcgcgcca tcgtagagtc cgcaagcgac tccatgtaca
     2521 tcgtggacag gtattgcagg tacctgttca tgaacggggc tcatcttaaa aggctcgacc
     2581 tcacgctcga agaggtcgtc ggcaggacct atggtgagtt ccattcggag gagcagaaga
     2641 gggaattcga ggatataatc aacgaagtcc tcacccatgg cctgcctgca aagcaggagc
     2701 accggagcgg ccgcgacagc cggtattttc tccggacctt caacctgctg aaaagcccct
     2761 cgggggagaa aaccaccgcc gccgtcgtcg tgatttcgaa agacatcaca gaatggaagc
     2821 aggcggaggt ggccctacgg gaaggcgagg aaaagtaccg cacgatcctc aacggcatcg
     2881 cggacggtta ctacgaagtc gacctacagg gcaatcttat gttcgtgaac ccttcgatga
     2941 ccagcatcct cggctactcc gaagaggaac tgctcggttt gaacaacagg cagtttatgg
     3001 acaaagaaaa cgcgagaacc gtttttcagg tcttcaacac cgtcta
//
LOCUS       DAOTMX010000213         3031 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_25375_length_3031_cov_3.758737, whole genome shotgun sequence.
ACCESSION   DAOTMX010000213 DAOTMX010000000
VERSION     DAOTMX010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25375_length_3031_cov_3.758737"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1311
                     /locus_tag="P5573_06540"
     CDS             <1..1311
                     /locus_tag="P5573_06540"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRT27752.1"
                     /translation="ADMFFLHLNLFPDDENRKDVLYALARSLMEMQQLGAALKLFGQV
                     IEQYPESKQAWESVVIMANLGVQQPGIKLPTFMSGASYYLDPIGTYDFLLRKFPIGDM
                     TEGLLFQRGQALLKAGRHREAFFNFAYMMQQFPGGRYRAEGANSLILSAERVVDDSYA
                     SGDYLFVADIYFKAYEKVMSRSRNLKARWMVGDSLKRVALPDEALKVFGDLLKEVGYQ
                     ERGRLLVEIADCHRRLGDDDQAEKVVAGVGNSRGTEPSVVAAAKRMLGDIYSRKGEYK
                     KAASLYGEVPAVRETLADRAVFHLNYGIALMETNALSTAKAQFLLAVESYEQEPKRYA
                     PDVLAVAHGGIGDCLLREKNYKEAIKMYRRASAALPEGSYNLWALQGMARGYAGIGDE
                     AQAQQLLSSLRDRGGEGFWTQLADYEQREGSWRAKYGKYLGSVD"
     gene            1357..2604
                     /locus_tag="P5573_06545"
     CDS             1357..2604
                     /locus_tag="P5573_06545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRT27753.1"
                     /translation="MDTPKHTGEPYEFLLRSFEDFNEATLRMQNAFRHLENRFEDINR
                     ELERKNLELEKTLAEKEEIKNDLLNILESLTTGVIVTDLRGRISRLNRCAEVFLGTPS
                     AHVQGRPLGDFLPGIVPGNPKADMGTYVGEREAKLQLRGRTVEVIRSPMKAPDGAVTG
                     SVLILRDVTRLEKLEEMAKRTERFAAMGEMAANIAHEIRNPLGSIALFASLLMKEERD
                     QRDLERLSHIVAAVRGMDNKISNLMIFARKQVPMMRVIGLQGVLKEVIGFSGEIVRQK
                     DVSVEAKYVRGNPRVMGDAEMLKQVFLNVILNAVQAMPDGGHLRIETSLRKNPEPAVE
                     VCFSDTGVGIPKEHLGRIFDPFFTCREGGTGLGLAIVHNIVDTHGGSIDVESGRGGTL
                     VRVSFPVVRSSRTRRVGRKVGGT"
     gene            2610..>3031
                     /locus_tag="P5573_06550"
     CDS             2610..>3031
                     /locus_tag="P5573_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRT27754.1"
                     /translation="MEGKAILIVDDEPSMRMALSESLVSCGYRVDTAVDGADGLNKFK
                     QGRYKAVITDMRMPKANGMDVLRGIKASSPQTHVIVITAYGTVSTAVEAMKNGASDFI
                     MKPFSLDELESVVRNVLADGMPADSALGRCREQDRNRE"
BASE COUNT          709 a          785 c          963 g          574 t
ORIGIN      
        1 gcagacatgt tcttcctcca cttgaacctc ttcccggacg acgaaaaccg caaggatgta
       61 ctctatgcat tggcccggtc gctgatggag atgcaacagt tgggggccgc cctgaagctg
      121 ttcggtcagg tcatagagca gtacccggag agcaagcagg cctgggagag cgtcgtcatc
      181 atggccaatc tcggcgtaca gcaaccggga ataaaactgc cgaccttcat gagcggtgct
      241 tcctattacc tggaccctat aggtacgtat gacttcctgc tgagaaaatt ccccatcgga
      301 gatatgacgg agggactcct tttccagaga ggtcaggcac tgttgaaggc gggcagacac
      361 agggaggctt tctttaactt tgcttacatg atgcagcagt ttccgggcgg gagataccgg
      421 gcggaaggcg ccaacagcct gatactgagc gccgagcgcg tggtcgacga ttcctatgcc
      481 tccggggact atcttttcgt ggccgacatc tatttcaagg cttatgaaaa ggtaatgagc
      541 aggagccgga atctcaaggc ccgctggatg gtcggcgaca gcctcaaacg ggttgcgctt
      601 cctgacgagg ccctcaaggt cttcggcgac ctcctgaaag aagtgggata tcaggaaagg
      661 ggccgcctcc tggtcgagat cgccgactgc catcgtcgcc tgggagacga cgaccaagca
      721 gaaaaggtag tcgccggggt cggcaactca cgggggacgg aaccttctgt cgttgccgcc
      781 gccaagagga tgctgggtga tatttacagt cgaaagggtg aatacaagaa ggcggcatcc
      841 ctgtacggag aagtcccggc cgtccgtgaa actctcgcag acagggctgt cttccacctc
      901 aactacggca tcgccctgat ggaaactaac gccctttcga ccgcgaaggc acaattcctg
      961 ctcgccgtag agagctatga gcaggagccg aagagatacg cacccgacgt tttagccgtc
     1021 gcccacggcg gcatcggcga ttgccttctc cgcgagaaga actacaaaga ggccataaag
     1081 atgtataggc gggcttcggc tgccctcccg gaggggtcgt acaacctctg ggcccttcaa
     1141 ggcatggcca ggggttatgc cgggatcggc gacgaggctc aagcgcagca gctcctttcc
     1201 tcgttgaggg acaggggtgg cgaggggttc tggacgcagt tggccgatta tgagcagaga
     1261 gaagggagct ggagggcgaa atacgggaag tacctcgggt ctgtcgactg agagggcccg
     1321 gagtcgccgt aatcgtaacg tcgacaggag acggtgatgg atacgcctaa acatacggga
     1381 gagccgtacg agtttctgtt gaggtctttc gaggacttca acgaagccac gttgcggatg
     1441 caaaacgcct tccgacacct ggaaaacaga ttcgaggata tcaatcgtga actggagagg
     1501 aaaaaccttg aattggagaa gaccctcgcc gagaaggaag agatcaagaa cgacctcttg
     1561 aacatcctcg agagcctgac gacgggggtc atcgtcaccg acctgcgcgg taggatttcc
     1621 cggctcaatc gttgtgccga agttttcctg ggaaccccgt cggcccacgt ccagggtagg
     1681 ccgctgggcg attttctccc cggaatcgtt cccggtaatc cgaaagcgga tatgggcaca
     1741 tacgtcggag agagagaggc gaagcttcag ttgagagggc gaacggtgga ggtgatccgc
     1801 tcgccgatga aggcccctga cggagccgtg acgggaagcg tgctgatcct gcgcgacgtc
     1861 acacgcctgg agaagttaga ggagatggcc aagaggacag aaaggttcgc ggccatgggt
     1921 gagatggcgg ctaatatcgc ccatgaaata agaaaccccc tcggcagcat agccctcttt
     1981 gcctctcttc tgatgaaaga agagcgggat caaagggacc tcgaaaggct ctcacatatc
     2041 gtcgcggccg tacggggaat ggacaacaag atatcgaacc tgatgatctt cgcccgtaaa
     2101 caggtcccga tgatgagggt catcggcctg caaggtgtat tgaaggaagt gatcgggttt
     2161 tccggggaga tagtgaggca gaaggatgtg tccgtcgagg caaaatacgt caggggaaac
     2221 ccgcgggtta tgggggatgc ggagatgttg aaacaagtat tcctcaacgt catcctcaat
     2281 gccgtccagg caatgcccga tggtgggcac ctgaggatcg agacgtccct acgcaaaaac
     2341 ccggagcccg ccgttgaagt gtgcttttcc gacacgggtg tcggcatccc gaaagagcac
     2401 cttggccgga tattcgaccc ctttttcacc tgccgagagg ggggcacagg cctaggtctc
     2461 gcgatcgtgc acaacatcgt cgacacccac ggcggttcaa tcgacgtgga gagcggccgg
     2521 ggagggacgc ttgtgagggt cagcttccct gtggtcagga gtagtaggac ccgtcgtgta
     2581 ggacgtaaag tgggaggtac gtaaagacca tggaaggcaa ggctattctg atagtcgacg
     2641 acgaaccttc tatgaggatg gctctttcgg agtcgctcgt gagttgcggc tatcgggtgg
     2701 atacggcggt ggacggcgcg gacggcttga acaagtttaa gcagggccgg tacaaagcgg
     2761 tcataacgga tatgcggatg ccgaaggcga acggcatgga tgtcctcagg ggcatcaagg
     2821 catcgtctcc ccagacgcac gtcatcgtca tcactgccta cggcactgtg agcaccgccg
     2881 tcgaggccat gaagaacggc gcctctgatt tcatcatgaa gcccttttcc ctggatgagc
     2941 tcgagagcgt ggtcagaaac gtcctcgccg acgggatgcc ggccgatagt gcgctcggta
     3001 gatgccggga acaggaccga aatagggaga t
//
LOCUS       DAOTMX010000214         3028 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_25416_length_3028_cov_3.534141, whole genome shotgun sequence.
ACCESSION   DAOTMX010000214 DAOTMX010000000
VERSION     DAOTMX010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25416_length_3028_cov_3.534141"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1045
                     /locus_tag="P5573_06555"
     CDS             <1..1045
                     /locus_tag="P5573_06555"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRT27755.1"
                     /translation="NLRQEIQQFIYRNKERYGYHNIIGKSEALQKVLEFLTKVANSDA
                     STILIEGASGTGKSLIAKCLHYNSLRAHQPFVEINCASIPGTLLESELFGYEAGAFTD
                     AKRQKKGLVEMAHGGTLFLDEIGEMEPALQAKVLQLIDEKCFRRVGGTKSIAIDVRIV
                     AATNRDLKKAVEDGFFRSDLYYRLNVIDIHLPSLRERKEDILPLVEHFIGHYSRKFNK
                     PVKRISEEARDLLTRYDWPGNVRELLNVVERVMILEDSGVLLPEHIPLRITKSGGNDH
                     SHATADADELLVEGGGSDYRSLTERFQSRLITRALGQARGNKSEAARMLGLSRLSLYH
                     QMKKLGMVKKGIS"
     gene            1395..2312
                     /locus_tag="P5573_06560"
     CDS             1395..2312
                     /locus_tag="P5573_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HRT27756.1"
                     /translation="MIKSLADFSRIAREKGPKKLAVLAPEDEEFMKAVKISWEVGYIE
                     PVLIGNEEKMERVAERVGFDIGKFRKIPGVDQQAISDLGVGMLFSGELSIVSKGQIPT
                     SYVYRSIIRQEAKAGSGMTVSVVTFWEIPGVDHLVAFTDTGVNIKPDLKAKREIINNA
                     VFVYHLMGYPRPKVAVLSGQREVGGVLPSYRDFLALKKSAASGDFGECEVVDATSFTG
                     ILLGGGVRMKDYSAVRLEEIPHILVVPCLDTGNIICKLDFFLDVTRSSLVATSRGPVC
                     VPARSDFSENIVQQIAMCVVLADRMEKGA"
     gene            2317..>3028
                     /locus_tag="P5573_06565"
     CDS             2317..>3028
                     /locus_tag="P5573_06565"
                     /inference="COORDINATES: protein motif:HMM:NF013665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HRT27757.1"
                     /translation="MIDSFKDILTAAKQGGLKKAVVPFPGREDIEVLSRAAALGIVSP
                     ILIGNEKSLKTFIGKTDLWSYPHEVLDEKDRKKALNRAIALMREGKGDILMQGGLTQR
                     ALMGAVLDKESGMKKARVVSYASVFQLLKREKLILVTDTFLNNNPGLVEKQAVLENAL
                     HLDRLLGVESPKVAVLAAVEQVNPGIPSTLDAAILSKMAERRQFGKALVEGPLDIDCA
                     LSQAAAGRKGVQSVVTGNV"
BASE COUNT          670 a          785 c          932 g          641 t
ORIGIN      
        1 aaacctgcgc caggaaatcc agcagtttat ctaccggaat aaggagcgtt acgggtacca
       61 caacatcatc ggcaagagtg aggccctcca gaaggttctc gagttcctga cgaaagtcgc
      121 caacagcgac gcctcgacga tcctgatcga aggggcgagc ggcacgggga agagcctcat
      181 cgccaaatgc ctccattaca acagcctgcg cgcccaccag ccgttcgtcg agatcaactg
      241 tgcctccata cccggtacat tgctggagag cgagttgttc ggttacgagg ccggggcctt
      301 cacggacgcc aagagacaga aaaagggtct cgtcgagatg gctcacgggg gtaccttgtt
      361 tcttgacgag atcggggaga tggaaccggc gctgcaggcg aaagtcctcc agctcatcga
      421 cgagaaatgt ttccggaggg tcggcgggac gaagagcatc gccatcgacg tccgcatagt
      481 tgcggcgacg aaccgggact tgaaaaaggc ggtcgaagac gggttctttc gcagcgacct
      541 ctactatcgg ttgaacgtca tcgacatcca cctgccgtcc ctgcgggaaa ggaaggagga
      601 catcttaccg cttgtcgagc atttcatcgg ccattacagc aggaagttca acaaaccggt
      661 caagcgaatc tctgaggaag cgcgcgacct gctcacccgg tatgattggc cgggcaatgt
      721 ccgcgagctg ctgaacgttg tcgagcgggt catgatactc gaagacagcg gggtgctcct
      781 gccggagcat atccccctga ggatcaccaa aagcgggggt aacgatcaca gtcatgctac
      841 ggcggacgcc gacgaactct tggtcgaggg tggtgggtct gattatcgat ctctgacgga
      901 gaggtttcag tcgcgcttga taacccgggc actgggtcag gcccgcggta acaagtcgga
      961 agcggcaagg atgctcggat tgtcccgcct gagtctgtac caccagatga agaaactggg
     1021 gatggtgaag aaggggatca gttgacaata gcaacagtat gttgaaaaat ttaacacctc
     1081 cggtcgaggc cgaggagatt tttttggaga caggtgcggg ctctgaagcg gtgaatcggt
     1141 tcagagccgg tcgggaagct ttacattgcg gcgattatta taaagaattt ctcatactaa
     1201 gatgttcgtt gcaggcccga tatttcatgg accggtgagt cccgtgggga ggctctctgt
     1261 gaaggcggta atggcacttc tcttgctaat gaagatagcg gttgtgtttc cctccctttt
     1321 tttgttcggt gtggggggta cctcggtcgg gtttccccta cactgattga aaactactga
     1381 ttaaggagtc ttcgatgatc aagtcgttag ctgatttttc ccggatagcc agggagaagg
     1441 gtccgaagaa gctggccgtt ctcgcacccg aggatgagga gttcatgaag gcggtcaaaa
     1501 taagctggga ggtggggtac atcgagcctg tcctcatcgg taacgaagag aagatggaac
     1561 gggttgcgga gagggtgggg ttcgacatcg gcaagttccg gaagattccc ggggtcgacc
     1621 aacaggccat ctccgacctc ggggtcggca tgcttttttc gggggagttg tcgatagtca
     1681 gcaagggcca gattcctacg tcttacgtct accgctcgat catccgtcag gaggccaagg
     1741 cagggtccgg gatgaccgtg agcgtggtga cattctggga gatacccggg gtcgaccatc
     1801 tggtggcctt caccgacacg ggtgtgaaca tcaaaccgga cctcaaggcc aaaagggaga
     1861 tcatcaataa cgccgtcttc gtttaccatc tcatggggta cccgaggccc aaggtcgccg
     1921 tcctttccgg tcagcgtgag gtaggcggtg tccttccttc ctacagggat tttctggcgt
     1981 tgaagaagtc tgccgcttcc ggtgatttcg gggaatgtga agtcgtcgac gccacttcct
     2041 tcaccggcat cctcctcggc ggcggggtaa ggatgaagga ttacagcgcc gtcaggctgg
     2101 aagagatacc ccacatcctg gtcgtaccct gtcttgacac gggcaacatc atctgtaagc
     2161 tcgatttttt cctcgacgtg acccgaagtt cccttgttgc cacctcgcgg gggcctgtat
     2221 gtgttcccgc gcggtccgat ttcagcgaaa atatagtcca gcagatcgcc atgtgcgttg
     2281 tgcttgccga ccgtatggag aagggagcgt gatgagatga tagattcctt caaagatatt
     2341 ctaacagctg ccaagcaagg cggtttgaaa aaggccgtcg tacctttccc cggtagagag
     2401 gacatagagg ttctttctcg ggctgccgcc ctcggtatcg tgtcgcctat cctgatcgga
     2461 aacgagaaat cgttgaagac cttcatcggc aagaccgatc tctggtccta cccccacgaa
     2521 gttctcgacg agaaagaccg gaagaaggcc ctgaatcggg cgatcgccct gatgcgtgag
     2581 ggcaagggcg acatcctgat gcagggcggc ctgacacagc gggccttgat gggtgccgtg
     2641 ctggataagg aaagcggcat gaaaaaggcc cgggttgtca gctatgcttc cgtcttccag
     2701 cttttgaaga gggagaagtt gatcctggtg actgatacct tcctgaacaa caacccgggg
     2761 ctcgtcgaga aacaggccgt cctcgagaac gccctccatc ttgaccggtt actgggcgtt
     2821 gaatcgccga aggtggcggt ccttgccgcg gtcgagcagg tcaatcccgg tatcccttcc
     2881 accctggacg cagctattct ctccaagatg gcggagcgca ggcaattcgg gaaggccttg
     2941 gtcgaggggc ccctcgacat agactgtgcc ctgagtcagg cggcggcggg acggaaaggc
     3001 gttcagagcg tcgtgaccgg caacgtcg
//
LOCUS       DAOTMX010000215         3021 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_25483_length_3021_cov_4.275792, whole genome shotgun sequence.
ACCESSION   DAOTMX010000215 DAOTMX010000000
VERSION     DAOTMX010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25483_length_3021_cov_4.275792"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="P5573_06570"
     CDS             <1..545
                     /locus_tag="P5573_06570"
                     /inference="COORDINATES: protein motif:HMM:NF012903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HRT27758.1"
                     /translation="GAGTVDLGNGLSITFQAGTFGEGDTFTVDAFALGYYRGNGEEMT
                     LNIGKDHTMHYSFSGEAVFTDKGAGTVDVFGTLNDLKAALAGNDAEGIRATLDGLKEA
                     QQQVTRYQAICGTRTNDLEVTKSNYEALKENITGLMSNVEDADVTKLITDFQMKQIAL
                     QASYYMAGEIGKISILNFIK"
     gene            564..797
                     /gene="csrA"
                     /locus_tag="P5573_06575"
     CDS             564..797
                     /gene="csrA"
                     /locus_tag="P5573_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HRT27759.1"
                     /translation="MLILTRKLGEAIAIGDDVKVHLLEIKGKQVRIGVEAPPRITVHR
                     EEIYRQIREQNQKSATPTGPAADLQEVWEKLKG"
     gene            809..1306
                     /locus_tag="P5573_06580"
     CDS             809..1306
                     /locus_tag="P5573_06580"
                     /inference="COORDINATES: protein motif:HMM:NF014660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HRT27760.1"
                     /translation="MELQTKLFGPIEVDTARVIEMRRPILGFERLRRYVLLIREEKTP
                     FGWLQSVEEAAVAFVVVNPFVVKGDYEPVLSDEVQGILGIERAEDVALLSIVTIRSEP
                     LEVSVNLRAPLVINVRNMTALQAVLEDETYPVRYVIKTIKERPAEIPQAVSLKISPTS
                     SDNFA"
     gene            complement(1255..1611)
                     /locus_tag="P5573_06585"
     CDS             complement(1255..1611)
                     /locus_tag="P5573_06585"
                     /inference="COORDINATES: protein motif:HMM:NF015602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HRT27761.1"
                     /translation="MNVNAVNTGGAVSPNYAVPVESGGVDDPGRKNNPEAKQAQTQPG
                     NADTKKMVAEMQARIDQMNISLTFSTYGKNKDRIAIVLSNKETGEVIREIPPEEIQKL
                     YAKLSELVGLIFNDTA"
     gene            complement(1640..2053)
                     /locus_tag="P5573_06590"
     CDS             complement(1640..2053)
                     /locus_tag="P5573_06590"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27762.1"
                     /translation="MITALRKKLKASEDFLACTLKLKDALEKEDSKATATLLEERAEL
                     IAVIDATERQIARLAPSPGERNPEDMRVLKKISEDISDRLRRASVVNDECDALAAGRI
                     ERLKKELAALSSEERGLKGYVHRGEQNPRFLDIQT"
     gene            complement(2062..2526)
                     /gene="fliS"
                     /locus_tag="P5573_06595"
     CDS             complement(2062..2526)
                     /gene="fliS"
                     /locus_tag="P5573_06595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HRT27763.1"
                     /translation="MYRSPKETYQESKFFTANPAELVLICYDGAIGNLRAAKAFYAEK
                     DFYSKAKALQKTFDIIHELNASLDMQRGGIIAANLRAIYSYLVRSLIEADLKADLDAF
                     DRTIKILEELASSWREIAKPKEEVKHEPSGMNPQMRQMQVGKPGRSAASAIA"
     gene            complement(2548..>3021)
                     /gene="fliD"
                     /locus_tag="P5573_06600"
     CDS             complement(2548..>3021)
                     /gene="fliD"
                     /locus_tag="P5573_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HRT27764.1"
                     /translation="TQKNDKLWTDGVVAVTAGVDVAGTINGEEATGVGQVLTGKSGQP
                     NVDGLVIKYTGTAQGVDIGTVKLTFGVAELYDRALFNMTDSIDGYLTFKQKSMQESIK
                     NYQTQIDEMEARLELKREQLLNNYARMEAALLKIQNQGNWLSGQLTAALSGWWSV"
BASE COUNT          617 a          826 c          824 g          754 t
ORIGIN      
        1 tgggggcagg aacggtcgac ctgggcaacg gcctctctat tacattccaa gctgggacct
       61 tcggtgaggg tgataccttt acggtggacg ccttcgccct tgggtattac cggggaaacg
      121 gcgaggagat gaccctgaat attggcaagg accataccat gcattacagt ttttccggcg
      181 aggcagtgtt tacagacaaa ggcgccggga cggtggatgt cttcggtacc ttgaacgact
      241 taaaggcggc gcttgccgga aacgacgccg agggaatcag ggcgacattg gacggcctga
      301 aggaggcgca gcagcaggtt acccgctatc aggccatctg tggcacccgg acgaacgacc
      361 tggaagtcac gaagagcaac tacgaggcgc tcaaggaaaa cataaccggc ctgatgtcga
      421 acgtcgaaga cgccgatgtc accaaattga tcactgattt ccagatgaaa cagatcgccc
      481 tgcaggcatc ttattacatg gcgggggaaa tcggaaagat atctatactt aacttcatca
      541 aatgatgccc tgcgggaggg gatatgctca tcctgacaag gaagttggga gaagcgatcg
      601 ctatcggcga cgatgtcaag gttcacctgc tggagataaa gggtaagcag gtcaggatcg
      661 gcgtggaggc acctccacgc atcaccgttc atcgggaaga aatctaccgg cagatccgcg
      721 agcagaatca aaagtcggcg actccgacgg gaccggcagc cgacctccag gaggtctggg
      781 aaaaattgaa gggatagtca gggactgtat ggaactacag actaagttat tcggaccgat
      841 agaggtagac acggcaaggg tcatcgagat gcgacggccg atccttgggt tcgaacgtct
      901 taggcgttat gtcctgctga ttcgggagga aaagacaccc ttcggctggt tgcagtccgt
      961 agaagaggcc gccgttgcct tcgtagtggt aaacccattc gtcgtgaagg gagactatga
     1021 gccggtactg agcgatgagg tgcagggaat cttgggtatc gagagggcgg aggatgtcgc
     1081 gctgctctcc atcgtcacga tcaggtccga acccttggag gtctcagtca acctgcgggc
     1141 gccgttggtg ataaacgtac gaaacatgac agcgttgcag gcggtcctgg aggacgagac
     1201 ctatcctgtt cggtacgtca ttaagacgat caaggaaagg cccgccgaaa tccctcaagc
     1261 cgtgtcgttg aagataagtc cgaccagttc ggataacttt gcgtaaagct tctggatctc
     1321 ctcgggcggg atttcccgga ttacctcgcc tgtctccttg ttcgagagga cgatcgctat
     1381 cctgtccttg tttttcccgt aagtagaaaa ggtcaggctt atgttcatct ggtcgatccg
     1441 ggcctgcatc tcggcaacca tttttttggt gtctgcgttg cctggttgag tctgagcctg
     1501 cttggcctcg gggttgttct tcctacccgg atcatcgaca ccgccggatt ccaccggcac
     1561 tgcataattg ggggaaacag ccccccccgt attgacggcg ttgacattca ttctttcacc
     1621 ccctttttct tcgtcctcct caggtttgga tgtccagaaa cctcgggttt tgttccccgc
     1681 ggtggacata acccttcaaa cctctctctt ccgaactcag agccgcgagc tcttttttca
     1741 atctttcgat tctgccggcg gcgagagcat cgcattcatc gtttacgaca gaggcgcgtc
     1801 tcaatctgtc gcttatatct tccgaaattt ttttcagtac ccgcatgtct tcagggttcc
     1861 tttctcccgg agaaggcgcc agacgggcta tttgcctttc cgtcgcatcg attaccgcga
     1921 tcaactccgc acgctcctcg agcagagtgg cggtcgcctt cgagtcttct ttctcgagtg
     1981 catccttgag cttcagtgtg cacgccaaaa aatcttcgct ggccttcaat tttttcctta
     2041 gggcggttat catctcgaac ctcacgctat tgccgaagcg gcacttctgc cgggttttcc
     2101 gacctgcatc tgcctcatct ggggattcat ccccgacggc tcatgtttta cttcttcttt
     2161 gggtttcgct atctccctcc aggaggaggc gagttcttcg agtatcttga tagtccggtc
     2221 gaaggcgtcg aggtccgcct tcaggtcggc ctcgatcaac gaccgtacaa gatagctgta
     2281 gatggccctg aggttcgcgg cgataatgcc gcctctttgc atgtcgagag aggcattcag
     2341 ttcgtggatg atatcgaagg tcttctggag cgccttagcc ttggaataga agtctttctc
     2401 ggcgtagaag gcctttgccg ccctgaggtt tccgattgcg ccgtcgtagc aaatcagtac
     2461 tagctcggcc gggtttgccg tgaaaaattt cgactcctgg tatgtctcct tcggactgcg
     2521 atacatagaa tcatctcctc ttttttatta tacgctccac caaccgctca aggctgcggt
     2581 gagctggccg gacagccagt tgccctggtt ctggatctta agcaaagccg cctccatccg
     2641 ggcgtaattg ttgagcagtt gctctctctt aagctccagc cttgcctcca tctcgtcgat
     2701 ctgcgtttgg tagtttttga tgctctcctg catggacttt tgcttgaacg tcaggtagcc
     2761 gtctatcgaa tcggtcatgt tgaagagcgc ccgatcgtag agctcggcta cgccgaaggt
     2821 cagttttact gttcctatgt cgacgccctg cgccgtcccc gtgtacttta tcaccaaacc
     2881 atctacgtta ggctgcccgg atttccccgt caagacctgc ccgacaccgg tcgcctcttc
     2941 gccgtttatc gtccccgcca cgtcgacgcc cgctgtgacg gccacaaccc cgtcggtcca
     3001 gagcttgtcg tttttttgtg t
//
LOCUS       DAOTMX010000216         3011 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_25591_length_3011_cov_3.596752, whole genome shotgun sequence.
ACCESSION   DAOTMX010000216 DAOTMX010000000
VERSION     DAOTMX010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25591_length_3011_cov_3.596752"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="P5573_06605"
     CDS             <1..433
                     /locus_tag="P5573_06605"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416620.1"
                     /note="catalyzes the formation of glutamate 5-phosphate
                     from glutamate in proline biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HRT27765.1"
                     /translation="YIIARGKKKGILRDIFAGKEIGTLFLPVADHLNSREYWIAYTLR
                     PRGKVYLDDGARNAILHHGKSLLPSGIVKVEGQFNMGDPVLCLDTEGKLIAKGLVNYN
                     SEEVEKIKGLKTSKIEQVLGYKHYDEVIHRDNMAVTRDKRL"
     gene            complement(514..1638)
                     /gene="uvrC"
                     /locus_tag="P5573_06610"
     CDS             complement(514..1638)
                     /gene="uvrC"
                     /locus_tag="P5573_06610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HRT27766.1"
                     /translation="MTDEIKDTLRKAPRDVGVYLMKDGGGRVLYVGKAKNLRSRVRSY
                     FGGNDSRPSIPFLVSKVRHIDFIVTNTEKEALILENNLIKEYRPRYNVYFRDDKDYIS
                     LRVDFERPFPRLEVVRRPEKDGAVYLGPYASSTAVKETLHFLQPIFPLRTCGEAEFRR
                     RKRPCLEYEIKRCLAPCVGRVSPEAYRRILEDAVTFLRGGERRLLSDLRRRMVREARE
                     RRFEEAARTRNMIVAIERTLEKQRVVSESRTNIDVFGLCREEGSTWTCVIYVRDGRLV
                     GRKAFPTVRSDMGPAAIISSLLKQYYDRGVSVPPEVLIPVDIEDKAVTEEWLSEKRGG
                     KVTVKRPLRGEKKALLDMALNNARQLFEAGKSPHESGEAT"
     gene            complement(1679..>3011)
                     /locus_tag="P5573_06615"
     CDS             complement(1679..>3011)
                     /locus_tag="P5573_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRT27767.1"
                     /translation="AISAVDPLLGRRIESLDRVTVYPGSHYVTTRDNRRRAIDSIRQE
                     LAERLAELKAQGKLVEAQRLEQRTKFDIEMMEEMGYCQGIENYSRHLTGRLPGEPPPT
                     LMDYLPEDALLVIDESHVTVPQLRGMYRGDRSRKETLVEYGFRLPSALDNRPLTFEEF
                     EKYPQQRLYVSATPAEYELKKAKGRVVEQIIRPTGLMDPEIRVKPVERQVEDLLGEIR
                     RRVERGERVLVTTLTKRMAENLTEHYQSLGLKVMYLHSDIATLERVAIIRDLRLGKYD
                     VLIGVNLLREGLDIPEVSLVAILDADKEGFLRSERSLIQTSGRAARNVAGEVIMYADR
                     VTDSIRVSLQETNRRREIQRRYNEEQGITPESIRKSVTDLLSSVYEADYYTVPVVSDK
                     EEVFLSEKDLLTMIRKLRSDMKRAAENLEFEQAARIRDRLRELEDLGLELG"
BASE COUNT          531 a          965 c          871 g          644 t
ORIGIN      
        1 gtacatcatc gcccggggca aaaaaaaagg catcctgcgc gatatcttcg ccggcaagga
       61 gataggcacg ctcttcttgc ccgtcgcgga ccacctgaac agccgcgaat actggatcgc
      121 ctacaccctg aggccgaggg gcaaggtata ccttgatgac ggggcgagaa atgccatcct
      181 gcaccatggc aagagcctct tgccctcggg catcgtgaag gtggaaggac agttcaatat
      241 gggcgacccc gtcctctgcc tcgacacgga gggcaagctg atcgccaagg ggctcgtcaa
      301 ctataactct gaggaagtcg agaagatcaa gggcctgaag acctcgaaga tcgaacaggt
      361 ccttggctac aaacactacg acgaggtcat acaccgggac aacatggccg tgacgcggga
      421 caagaggtta tgaacatcac tgtccgcagc gcgaccctgg aggcgacgaa aggccgtttt
      481 tcggcgcctc cagggggttc aggcccggca gggtcaggtg gcctcgccgc tttcatgggg
      541 gcttttcccc gcctcgaaaa gttgccttgc attattcagg gccatgtcca ggagggcctt
      601 tttctctccc cttaggggcc tcttgactgt gacctttcct ccccgctttt ccgagagcca
      661 ctcttccgtg acggccttgt cttcgatgtc gacggggatc aggacctccg ggggcacgga
      721 tacgccccgg tcatagtact gtttgaggag cgatgagatg atggccgccg ggcccatatc
      781 acttctcacg gtggggaagg ccttcctgcc cacgaggcgg ccgtcgcgga cgtagatgac
      841 acaggtccac gtcgaccctt cctcacggca aagaccgaag acgtcgatgt tcgtccttga
      901 ctcggagacg acccgctgtt tctccaaggt cctttcgatg gcgacgatca tgttcctggt
      961 cctagccgct tcctcgaagc ggcgttccct cgcctccctc accatcctcc ggcggaggtc
     1021 ggagagaagc cgcctctctc ctccgcgaag gaaagtcacg gcgtcttcga gaatacgccg
     1081 gtaggcctcg ggactcaccc ttccgacgca gggcgccaga cagcgcttga tctcgtactc
     1141 caggcagggc ctcttgcgcc tcctgaattc cgcctctccg caggtcctga gcgggaagat
     1201 cggctggagg aaatggaggg tctccttcac cgccgtgctt gaggcatagg ggccgaggta
     1261 gacggcgccg tccttttccg gccgccggac cacctccagg cggggaaagg ggcgctcgaa
     1321 gtcgacccgc agggagatgt aatccttgtc gtcccggaag tagacgttgt aacgcggcct
     1381 gtattccttg atcaggttgt tttcgaggat gagggcctct ttctccgtgt tggtgacgat
     1441 gaaatctata tggcggacct tcgacaccag aaaggggatc gagggacgcg aatcgttccc
     1501 gccgaagtaa gaacggacgc ggctcctgag gttcttcgcc ttccccacgt aaagtacccg
     1561 cccgccgccg tccttcatca gataaacgcc gacgtcccgc ggggccttgc ggagtgtatc
     1621 cttgatttca tccgtcatcg acatcggccc gtattcacgc tcctgtaatc ttctgccgtc
     1681 atcctaattc gagccccagg tcttcaagct ccctgagcct gtccctgatc ctggcggcct
     1741 gttcaaactc caggttttcg gcggcccttt tcatgtccga cctcaatttc cggatcatcg
     1801 tgaggaggtc cttctcggag aggaacacct cctccttgtc ggatacaacc ggcacggtgt
     1861 aataatcggc ctcgtagacg gacgacagga ggtcggtgac ggatttcctt atcgactccg
     1921 gtgtgatgcc ctgttcttcg ttgtacctcc gctgtatttc ccgccgccgg ttcgtttcct
     1981 ggagactgac gcggatgctg tcggtgaccc tgtccgcgta catgatgacc tccccggcga
     2041 cgttccgggc ggcccgcccg ctcgtctgga tgagggaacg ctcggaacgc aggaaacctt
     2101 ccttgtcggc gtcgaggatc gccaccaggg agacctccgg gatgtcgagt ccctcccgca
     2161 gcaagttcac gccgatgagg acgtcgtatt tccccagccg caggtcgcgg atgatcgcca
     2221 cccgctccag ggtggcgatg tcggagtgga gatacatcac cttgaggccg agactctggt
     2281 agtgctccgt gaggttttcg gccatccttt tcgtcagggt cgtcacgagg accctctccc
     2341 ccctttcgac gcgcctgcgt atctccccca acaggtcctc cacctggcgc tctacgggct
     2401 tcacccttat ttccgggtcc atgaggcccg tcggcctgat gatctgctct accacacgcc
     2461 ctttcgcctt cttcaactca tactcggccg gtgtcgccga gacgtacagg cgctgctggg
     2521 ggtatttctc gaactcctcg aaggtgagcg gccggttgtc cagggccgag ggaagacgga
     2581 atccgtattc caccagcgtt tccttccggg agcggtctcc ccggtacatc ccccgcagct
     2641 ggggaacggt cacgtgactc tcgtcgatga cgagcagcgc gtcctccggc aggtaatcca
     2701 tgagcgtcgg cgggggctct cccggcagcc tgcccgtcag atggcgggag tagttctcga
     2761 tcccctggca gtagcccatc tcctccatca tctcgatgtc gaatttcgtc cgttgctcga
     2821 gacgttgggc ctcgacgagc tttccctgcg ccttcagctc cgccagtctt tccgccagct
     2881 cctgccggat gctgtcgatg gcgcgccgcc tgttgtcccg cgtggtgaca taatgactcc
     2941 ctggatagac ggtcacccgg tccaggctct ctatcctcct gcccaggagc gggtccactg
     3001 cagaaatagc c
//
LOCUS       DAOTMX010000217         3008 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_25630_length_3008_cov_3.591263, whole genome shotgun sequence.
ACCESSION   DAOTMX010000217 DAOTMX010000000
VERSION     DAOTMX010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25630_length_3008_cov_3.591263"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>228)
                     /locus_tag="P5573_06620"
     CDS             complement(<1..>228)
                     /locus_tag="P5573_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27768.1"
                     /translation="LQSERDAFQKQTTELQALLQAVYNSTSWRITAPMRRAKRLLMKV
                     LPGIRRVSVEAVRLPSRLVRPVLAKTAQWAA"
     assembly_gap    229..238
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<239..1109)
                     /locus_tag="P5573_06625"
     CDS             complement(<239..1109)
                     /locus_tag="P5573_06625"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRT27769.1"
                     /translation="MGSGFYKAFEDRHRGSRDLIKERLRVYLPFVEPLKKLYPECRAV
                     DLGCGRGEWLELLGEVGIEACGVDIDADMVAECRKLGLNAEEGDAISFLRSMPDESQA
                     VVSAIHVVEHLPFPEVQTLATEAMRVLKPAGLLILETPNPENLVVAGYHFYVDPTHMR
                     PVPPQLLSFLTEYCGYCRTKILRPREDPSLAVDRPLGVLDVLGGVSADYAVVAQKEAP
                     EECLAPFDRAFGAAQGMNIESLAGRYDAQQNEKIQSIGGMLNEKIDGIREILNRQMTT
                     LQTERDVLQATLQA"
     gene            complement(1510..1800)
                     /locus_tag="P5573_06630"
     CDS             complement(1510..1800)
                     /locus_tag="P5573_06630"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HRT27770.1"
                     /translation="MNKQPAVYILASKRNGTLYIGVTSDLLKRIWEHRNDMVEGFTRR
                     YGVHRLVWYEVHESMESAIEREKRLKEWKRKWKLELIERSNPDWRDLYDMIV"
     gene            complement(2117..>3008)
                     /locus_tag="P5573_06635"
     CDS             complement(2117..>3008)
                     /locus_tag="P5573_06635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HRT27771.1"
                     /translation="MAAQEEFFRARRAKFETDRKIVMESLTGFRHYSAQLNQQKVSYE
                     KQLMIIRQQLDSLKGLAEEGYYAKNRFLDLERAAEDLRGRIAETSANQLRAEATVQEY
                     MMRLSAIERDYLKDVKAELADVEKKLPGIRDAYYAIKDMLEKTEIKAPDDGIVMGLRV
                     HTIGGVVSPGQPILEIVPKDSELIVEAKLSPANVEDIRKGQTADLHFTALDPKKTPVF
                     EGTVIYVSPDVMTDEVTKQPYYLVRVKINEETLKKIKELNKEISAGMPVQVVIKTGSR
                     TFLSFLFKPFIDRLAISFLR"
BASE COUNT          621 a          950 c          737 g          690 t
ORIGIN      
        1 cggcggccca ctgagccgtc ttagccaaga ccggccgcac aagccgcgaa ggcagccgca
       61 ctgcctccac cgatacccgc cggatgcccg gcaggacctt catcagcaat ctcttggcac
      121 gccgcatcgg ggcggtgata cgccaggacg tgctgttgta aacggcctgc agcagcgcct
      181 gaagttccgt cgtctgcttc tggaaggcgt cccgctccga ttgcagtgnn nnnnnnnncg
      241 gcctgcagcg tcgcctgaag gacatcccgc tccgtctgca atgtcgtcat ctgtcgattg
      301 aggatctccc tgatgccgtc gattttctcg ttgagcatac cgccgatact ctggattttc
      361 tcgttctgct gggcgtcgta gcgccccgcc aatgactcga tattcatccc ctgcgcggcg
      421 ccgaaggcgc ggtcgaaggg cgcaagacat tcctcaggcg cctccttctg cgcgacgacg
      481 gcgtagtcgg cgctcacgcc cccgaggacg tccagaacgc ccaatggcct atcgacggca
      541 agagaagggt cttcccgggg gcggagtatc ttcgttcggc aatagccgca gtactcggta
      601 aggaaggaga gcaactgcgg aggcaccggg cgcatgtgcg tcgggtcgac gtaaaagtgg
      661 taacccgcaa cgacgaggtt ctccgggttc ggcgtctcga gtatcaacag accggccggc
      721 ttcagaacgc gcatcgcctc cgtcgccagg gtctgtacct ccgggaaggg caagtgctcc
      781 acgacatgga tggccgagac gacggcctga ctttcatcgg gcatacttct gaggaaagag
      841 atcgcgtctc cctcctctgc gttcagtccc agcttgcggc attcggccac catgtcggcg
      901 tcgatgtcga cgccgcacgc ctcgataccc acctcgccga gcaactccag ccactcgccg
      961 cgaccgcaac cgaggtctac cgccctgcat tcgggatata gtttcttcag cggttcgacg
     1021 aagggcaggt agacccgcag ccgctccttg atcaggtctc gcgagccccg atgcctgtcc
     1081 tcgaaggcct tataaaatcc tgaacccatc aaacaaatcc caacgtcact ccgatcccct
     1141 tcgccacccc ggctctcttt ttgtcattcc ggcgcgtcac ccccctaacg tcaccccggc
     1201 gaaagccggg gtccaaaaaa cgaaaatcgg gggcggaatc cagtcctctt cgtcattccg
     1261 acgaaagtcg gaatccagta aatgaaagtc ttcgcggtat ccagtcatga cccgtaaaaa
     1321 gtccaaaaac gtcatgccgg acttgattaa gcctgccccc gtatcggagc ctgccccgta
     1381 cccgatacgg ggggcaggct ccgatacggg ggcatccaga acatattgaa tatactggat
     1441 tccggcgttc gccggaatga cgaaacgcgt aattctcgac tttttacgag tccatcagtc
     1501 atgcttctgt caaacaatca tatcgtacaa atcccgccaa tccggattac tcctttcaat
     1561 caactccagc ttccacttcc tcttccattc cttcaatcgc ttctcccgtt cgatagcgga
     1621 ttccatactt tcgtgcactt cataccaaac caggcgatga acaccgtaac gtctggtaaa
     1681 tccttccacc atgtcgtttc tatgctccca aatccttttg aggaggtctg aagtcacgcc
     1741 aatataaaga gtaccatttc gcttgctcgc taagatgtaa accgcaggct gcttgttcat
     1801 tgacacatct ctggattccg gcattcgccg gaatgacgag ctaaaggcat ataaaaggca
     1861 gtacgatggc ttaattttct caccctgccc ctctcccgtc ataccggcga aagccggtat
     1921 ccagtgcttt caatatgtcc tggattccgg cattcgccgg aatgacgagc taaaggcata
     1981 tgcccgccgg catgacgaaa aaatgcccct ggactacccg tatcaaagtc tgccccgtac
     2041 tcctcctgtc gagcacgggg cagaataaat tccagcacac agaactgcta tgacatgccc
     2101 cggcttcccc ctctacttac ctgagaaaag aaatggcgag gcgatcgata aagggtttga
     2161 aaagaaagct caagaatgtc ctgctgcctg tcttgatcac cacctggaca ggcatacccg
     2221 cactgatctc tttattcaac tcctttatct ttttgagggt ctcctcgttg atcttcactc
     2281 tcacgaggta gtacggctgc ttcgtaactt cgtcggtcat cacgtccggg gagacataga
     2341 ttaccgtgcc ttcgaacacc ggtgtctttt tagggtcgag ggccgtgaaa tgaaggtccg
     2401 cggtctgccc ctttcttatg tcctccacat tagcgggaga aagcttcgcc tcgacgataa
     2461 gctccgagtc cttcgggact atctcgagga tcggctgccc cggcgacacc acccctccta
     2521 tcgtatgcac cctcagcccc atgacaatcc cgtcatcagg ggccttaatc tccgtttttt
     2581 cgagcatatc ttttatcgca tagtaggcat ccctgatacc cggcaacttt ttctcgacat
     2641 ccgcaagctc cgccttgacg tccttcagat agtccctttc gatcgcgctg agcctcatca
     2701 tgtattcctg gaccgttgcc tccgccctga gttggtttgc ggaggtctct gcgatccttc
     2761 ccctcaaatc ctccgcggcc ctttcgaggt cgaggaacct gttcttggcg tagtatcctt
     2821 cttcggcgag ccctttgagg gaatcgagct gctgtcttat gatcataagc tgcttttcat
     2881 aagaaacctt ctgctgattg agctgggccg agtagtgcct gaatcccgtc agggactcca
     2941 tgacgatttt cctgtccgtc tcgaacttcg cccttctcgc ccggaagaac tcctcctggg
     3001 cggccatc
//
LOCUS       DAOTMX010000218         2991 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_25814_length_2991_cov_3.462193, whole genome shotgun sequence.
ACCESSION   DAOTMX010000218 DAOTMX010000000
VERSION     DAOTMX010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25814_length_2991_cov_3.462193"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /gene="dapA"
                     /locus_tag="P5573_06640"
     CDS             complement(<1..726)
                     /gene="dapA"
                     /locus_tag="P5573_06640"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041584832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HRT27772.1"
                     /translation="MFKGAIVAIVTPFKNGKVDEKALRELIEFQIENGTDGIVPCGTT
                     GESPVLSHDEHDKVIEITVDAVKKRVPVIAGTGSNSTEEALRLTRHAYEVGADGALMV
                     CPYYNRPTQEGLYQHYKFIAENVPIPIVIYNIPGRTGVNMTPETLARLAKMKNIVGVK
                     EAAGSLQQMADIISLCGPDFSVLSGDDFFTFPLLCLGGHGIISVVSNVAPADMAAMVD
                     SFSAGDINKARELHYKTTPLVKAL"
     gene            complement(774..1592)
                     /gene="dapF"
                     /locus_tag="P5573_06645"
     CDS             complement(774..1592)
                     /gene="dapF"
                     /locus_tag="P5573_06645"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HRT27773.1"
                     /translation="MIEFFKMSGTGNDFILIDDRKDTLPIQDIRTFAVRVCSRKVSVG
                     ADGLIIIKHSDRADFKWLFFNSDGSEAEMCGNGGRCAARFAFLKGIAGSSLSFETRAG
                     VIDAEVYGEIVKLRLTDPKDLRVDYTLTANGHSFVLSSINTGVPHAVMFTEELREFDV
                     YNTGRMIRYHDAHKPAGTNADFVKILDRHAIQVRTYERGVEDETLACGTGAVASALIA
                     SRKGLVEPPVDVQVKSGERLKVHFELSQEGFGNVYLEGAARVIYEGFLWKEAYE"
     gene            complement(1617..2873)
                     /gene="lysA"
                     /locus_tag="P5573_06650"
     CDS             complement(1617..2873)
                     /gene="lysA"
                     /locus_tag="P5573_06650"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HRT27774.1"
                     /translation="MHDFKYVNDELYCEGVSVSRIAEDIGTPFYLYSTRTLTNHYRVF
                     DRAFSGLRHIVCYSVKANSNVAILKLLVKEGAGVDIVSGGELYRALQAGVEPGKVVYS
                     GVGKRVDEIEFALATDILMFNIESSEELMVIDRCAGRMKKKARIAVRINPDVDPRTHP
                     HISTGLKENKFGVNIEKSLETYRWARTLGNIEVVGVDCHIGSQVIQIEPFVDALKRLK
                     KLMGLLEKEGVRIGYLDLGGGLGITYDQELPPHPEEYGRAVKEVVKDTDVTVILEPGR
                     VIVGNAGILVTRVLYRKQNENKNFVIVDAGMNDLIRPSLYGSYHALQPVVRKDREEFI
                     ADIVGPICESGDFIAKSRPVPKLEGGELLAVMSAGAYGFSMSSNYNSRPRIPEVLINN
                     ESYYVIRKRETYADLVRGEEIPDFLE"
BASE COUNT          644 a          894 c          716 g          737 t
ORIGIN      
        1 gagggccttc acgagcgggg ttgttttgta gtgaagctct ctcgccttat tgatgtcgcc
       61 ggcgctgaag ctatcgacca ttgcggccat gtcggcagga gccacgttgg aaacgaccga
      121 gatgattccg tgaccgccca gacagaggag aggaaaggtg aaaaagtcgt ctcccgacag
      181 caccgagaaa tcgggaccgc agaggctgat gatatccgcc atctgctgaa gcgaaccggc
      241 ggcttctttc acgccgacga tgttcttcat cttcgcgagc cttgccaggg tctcgggcgt
      301 catgttgacc cccgtcctcc ccgggatgtt gtagataacg atcgggatgg gtacgttttc
      361 cgcgatgaat ttatagtgct ggtagagtcc ttcctgggtc ggcctgttgt aataaggaca
      421 gaccatcaac gccccgtctg ctcctacctc gtaggcatgc ctcgtcaacc tgagggcctc
      481 ttcggtgctg ttagaccccg tcccggcgat gacgggaacc ctctttttga ccgcatccac
      541 cgtgatctcg atgaccttgt catgctcgtc atgagaaaga acaggagatt cgccggtcgt
      601 accgcagggt acaatgccgt cagtgccgtt ctcaatctgg aattcgatca gctctcgaag
      661 agccttctcg tccaccttcc cgttcttgaa gggcgttacg atcgctacta tggcaccctt
      721 aaacataatc ttctcctcct ataaaatgaa attgatacct aagactggaa gcctcactca
      781 taagcttcct tccaaaggaa gccttcatat attacccgtg ctgcaccttc gaggtaaaca
      841 tttccgaaac cctcttgcga taactcgaaa tgtactttta atctctcacc actttttacc
      901 tgaacatcta caggaggttc aacaaggcct ttccgggacg caatcagggc tgacgcaaca
      961 gctcccgtgc cgcaggccag cgtttcatct tcgacgccgc gctcataagt cctgacctgg
     1021 atcgcatggc ggtccaggat cttgacaaaa tcggcgttag ttcccgcagg cttatgagcg
     1081 tcatgatacc ggatcatccg cccggtattg taaacgtcga attctctcag ttcctccgtg
     1141 aacatgacgg cgtgcggcac gccggtattg atgctgctga gcacgaagga atgcccgttt
     1201 gcagtcaggg tataatcgac ccgcaaatcc ttcgggtccg tcaggcgcaa cttgacgatt
     1261 tctccgtaga cctccgcgtc gatgacgccg gccctcgtct caaaagatag actgctgccc
     1321 gcgattccct tcaggaatgc aaaccgcgcc gcgcaccttc ccccgtttcc gcacatctcc
     1381 gcctcgctgc cgtcggaatt gaaaaaaagc cacttaaaat ccgcccggtc cgagtgcttt
     1441 atgattatta agccatcagc cccgacggag acctttctgc tgcagacccg gacggcaaag
     1501 gtccttatat cctgaatggg caacgtgtcc ttccggtcgt ctatcaggat aaaatcattc
     1561 cccgtcccgc tcattttgaa aaattctatc atccgcccgg acccctcagg cttcactcat
     1621 tccaaaaaat cgggtatctc ttcaccccgg actaggtctg cgtatgtctc tcttttcctg
     1681 atgacatagt agctttcatt gttgatcaag acctcgggga tccgtggccg ggagttgtag
     1741 ttggaggaca tactgaagcc gtacgccccg gcgctcatca cggccagaag ttctcccccc
     1801 tccagcttgg gaacgggacg ggatttggcg atgaaatcgc ccgattcaca tatcggaccc
     1861 acgatgtctg cgatgaactc ctcgcgatcc ttcctcacga ccggctggag ggcgtggtaa
     1921 gagccgtata gactcgggcg gatcaagtcg ttcatccccg catcgacgat gacgaagttt
     1981 ttgttttcgt tctgtttccg gtatagaacc cgcgtcacca ggattcctgc gttccccacg
     2041 atcaccctgc ccggctccaa aataacggtg acgtcggtgt ccttcaccac ttctttgacg
     2101 gcccggccgt attcttccgg atgaggtgga agttcctgat cataggtgat cccaagaccg
     2161 cctccaaggt ccaggtatcc gatccgtaca ccctcttttt ccaggagacc catcagtttt
     2221 ttcagtcttt tcagtgcatc gacgaacggt tcaatctgga tgacctgcga acctatatga
     2281 caatcaaccc cgacaacttc gatgttcccg agtgtcctcg cccatcgata ggtctcaagc
     2341 gatttctcga tgttgacccc gaatttgttt tccttcaagc cggtggagat atgagggtgg
     2401 gtcctcgggt ccacgtcggg gtttatcctg acagcaatcc ttgccttttt cttcatcctc
     2461 ccggcgcatc tatcgataac catcaactct tccgacgatt cgatgttgaa catgagtatg
     2521 tccgtcgcaa gagcaaactc gatctcatcc acccttttgc ccacacccga gtacacgacc
     2581 ttgcccggct ccacgccggc ctgcaaggcg cggtacagtt caccgccgga aacgatgtcg
     2641 acccctgcac cttctttgac gagcaacttc aggatcgcga cgtttgaatt cgccttgacg
     2701 gagtaacaga cgatatggcg caacccgctg aatgcacggt cgaagacacg gtagtgattg
     2761 gtaagcgtcc tcgtactgta cagataaaaa ggcgtaccga tgtcttcggc gatgcgactt
     2821 acggacactc cttcgcaata gagttcatcg ttcacgtact tgaagtcgtg catggcaagt
     2881 cagcctactc ctttactttc atctccgcca tgttcgattc ggcaccgcaa ttcccatggt
     2941 cgtcgcagat cactacctta taagaataca accttccact cttcagtgct g
//
LOCUS       DAOTMX010000219         2983 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_25894_length_2983_cov_4.496585, whole genome shotgun sequence.
ACCESSION   DAOTMX010000219 DAOTMX010000000
VERSION     DAOTMX010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25894_length_2983_cov_4.496585"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="P5573_06655"
     CDS             <1..311
                     /locus_tag="P5573_06655"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRT27775.1"
                     /translation="VKYNRSEKPRIDIRFVPRKRRLQIRFEDNGIGIAKKETKKIFRK
                     FYQVGRSEDMTARGSGLGLYLVRHIARIHKGKIGAESRGIGKGSTFTLTLPLQKSQVA
                     "
     gene            324..1079
                     /locus_tag="P5573_06660"
     CDS             324..1079
                     /locus_tag="P5573_06660"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRT27776.1"
                     /translation="MKESSDKRVLIVEDDEHIAVGLKLNLSLRGYEVMVAPDGVSGLQ
                     KWKEWQPHLVILDIMLPGIDGFSVLRSIRLTDERLPILILTAKGEPDDKVKGLSCGVD
                     DYMTKPFNLDEFLLRVERLLTRGSWVRREESVEDPSSRTYSFGGNRIDFNAHSAQCKL
                     GKIALTEQELKLLRLFIANRGRPLSRKQILEIGWGYMGVTETRTVDNFIVRFRKYFED
                     DPHNPAYFRSIRSVGYVFDHSETNDEGRDTAGR"
     gene            1097..2020
                     /locus_tag="P5573_06665"
     CDS             1097..2020
                     /locus_tag="P5573_06665"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRT27777.1"
                     /translation="MSSNKKSTGLAVLALALMSLIWGYNWIIMKEALKYCSAFDFAAM
                     RTFFGALVLFSFLVTTGRNLRPQQVPLTALLGLLTTTGCVGLSTWALETGGAGKTAVL
                     VYAMPFWLLLLSRPLLGERIGWIQRVAVALALIGLVAILEPWHTDGSVQGSLLAVSSG
                     ISWAGGAVALKIIHRRATDFDLVSITTWQLFFGSLPLVAVALVAPDRPIDWTPYLAGA
                     VLFNIVFTSIVALLLWFYSLRELPAGIAGMGTLATPVIGVVAAAVQLGESPSLWEFSG
                     MTLIMAGLVLITLPGLLRYRRLRDIITGGST"
     gene            2093..>2983
                     /locus_tag="P5573_06670"
     CDS             2093..>2983
                     /locus_tag="P5573_06670"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HRT27778.1"
                     /translation="MEPTRNLKALFQPKSVALIGASGTPGKLGYDILHNLMQAGFEGP
                     IYPINPKADEILGLPAYKDVGSTPAPPELAVIVIPSKMVVGAVEECGKAGVRAAIVIT
                     GGFAEAGQEGERLQAELARTAEKYGVRVVGPNCQGINSPHHHLCASWPLITTQGRIAF
                     ISQSGTVGAALIDWASQEELGFSVFVSLGNRADVCESDCIDYFNKDPNTKVIALYIEG
                     VKRPQHFLQTLENATKPVVILKSGRTARGRIAAESHTKSLAGSDAVYEAIFRKYKVRR
                     ADSLEELYDFAKAMAYMQASG"
BASE COUNT          655 a          886 c          853 g          589 t
ORIGIN      
        1 ccgtcaaata caaccgctcg gagaaaccgc gcatcgatat ccgttttgtg ccgcggaaac
       61 gcaggctgca gatccgcttc gaagacaacg gcatcggcat cgcgaagaag gaaaccaaaa
      121 agatcttccg taaattctac caggtcggcc ggtcggaaga catgaccgca aggggaagcg
      181 gactcggcct gtacctcgta agacacatcg cccggataca caagggaaag ataggcgccg
      241 agagcagggg aatcggaaag gggtcgacct tcaccctgac cttgccgttg cagaagagtc
      301 aggtcgcatg aaaagaaagg gccatgaagg aatcgtcgga taagagggtc ctcatcgtcg
      361 aggacgacga acacatcgcg gtcggcctaa aactcaacct ttctctccgc ggctatgagg
      421 tcatggtcgc cccggacggg gtttcgggcc tgcaaaagtg gaaggaatgg cagccccatc
      481 tcgtcatcct tgacatcatg ctccccggaa tcgacggttt ctctgtcctg cgtagtatcc
      541 gtcttaccga tgagcgcctt cctatcctga tcctaacggc caagggtgaa ccggacgaca
      601 aagtgaaagg cctctcatgc ggcgtcgacg attacatgac caagcccttc aacctcgacg
      661 aattccttct cagggtggag cgcctcctga ccaggggttc ttgggtccgc cgtgaagagt
      721 cggtagaaga cccgtcgtcg cgtacctatt ccttcggcgg caaccgtata gacttcaatg
      781 cccacagtgc gcagtgcaag cttggcaaaa ttgcattgac ggagcaggaa ctgaagttac
      841 tgaggctttt catcgccaac cgcggcaggc cgctttcgag aaaacagata ctcgagatcg
      901 gatggggcta catgggtgtc acggaaacgc gcaccgtcga caatttcatc gtccggtttc
      961 ggaagtattt cgaggacgac ccgcataacc ccgcctattt caggagcatc cgttcggtgg
     1021 gttacgtctt cgaccattcc gagaccaacg atgaaggccg agatacagca gggagataaa
     1081 ggagcatctc cgaaaattga gctcaaacaa aaaatccacc gggctcgccg tcttggcgct
     1141 cgcccttatg tccctcatct ggggttacaa ctggataatc atgaaggagg cgctcaagta
     1201 ttgctctgcc tttgattttg ccgccatgag gacgttcttc ggcgcccttg tcctgttttc
     1261 tttcctcgtc accacaggca ggaatctccg gccgcagcag gtaccgctga ccgccctatt
     1321 ggggctgctg acgacaaccg gctgcgtcgg cctatcaacc tgggcactgg agaccggagg
     1381 cgccggcaag acagcggtcc tggtgtacgc catgcccttc tggctccttc tcctctcccg
     1441 gccgttactc ggcgaacgca tcgggtggat acagcgggtc gccgtggcac tggcgctcat
     1501 cgggctcgtc gccatcttag agccctggca tacggacgga agcgtacaag gaagcctgct
     1561 ggccgtttct tcgggaattt cctgggccgg gggcgccgtt gccctgaaga taatccaccg
     1621 gagggcaacg gacttcgacc tggtctccat aaccacctgg cagcttttct tcggttccct
     1681 ccccttggtc gcagtcgccc tcgtcgcccc ggataggccg atcgattgga ctccttacct
     1741 cgcgggggcg gtccttttca acatcgtctt caccagtatc gtcgccctgc tgctgtggtt
     1801 ctacagcctt cgggagcttc ccgcaggaat cgccggcatg ggcaccctgg caacccccgt
     1861 catcggcgtt gtggcagcgg ctgttcagct cggcgagagc ccctctcttt gggagttttc
     1921 cggcatgaca ctcatcatgg ccggccttgt cctcatcacc ctgcctggat tgctccgata
     1981 ccgacgtctg cgggatatca tcacaggcgg atcgacttaa tcgtagaaaa tgaaaagggg
     2041 ctatggtagt tttccgtatg gtgcaactat ccacaaaaaa ggaggaggag ctatggagcc
     2101 gaccagaaac ctgaaggcac ttttccaacc caaaagcgtt gccctcatcg gggcatcggg
     2161 gacgccgggc aagctgggtt acgacatcct tcataacctg atgcaggccg ggttcgaagg
     2221 accgatctac cccataaacc cgaaagccga tgaaatcctc ggcctcccgg cttacaaaga
     2281 cgtcggttcc acaccggccc ctccggagct ggcggtcatc gtcattccgt ctaagatggt
     2341 ggtcggggcc gtcgaagaat gcggaaaagc gggcgtaagg gcagccattg tcataaccgg
     2401 cggtttcgcc gaggccggcc aagaagggga gaggcttcag gctgagcttg caaggacggc
     2461 tgagaaatac ggcgtccgcg tcgtgggccc caactgccag gggatcaaca gccctcacca
     2521 tcacctttgc gcctcctggc ccctgatcac aacacaggga aggatcgcct tcatctccca
     2581 gagcgggaca gtgggggccg ccctgatcga ctgggcgagt caggaagagc tcggtttcag
     2641 cgttttcgtg agcctcggaa accgggccga cgtctgcgaa tcggactgta tagactactt
     2701 caacaaagac cccaacacga aggtcattgc cctgtacatc gaaggtgtca agaggccgca
     2761 gcatttcctc cagaccctgg aaaatgcgac caagcccgtg gtgatcttga agtcagggcg
     2821 cacggcgagg gggcgcatcg ccgccgaaag ccacacgaag tctctcgccg ggagcgacgc
     2881 cgtctacgag gccatcttca ggaaatacaa agtccgtcgg gccgacagcc tggaagaact
     2941 ttatgacttt gccaaggcca tggcatacat gcaggctagc ggc
//
LOCUS       DAOTMX010000220         2972 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_26015_length_2972_cov_2.658210, whole genome shotgun sequence.
ACCESSION   DAOTMX010000220 DAOTMX010000000
VERSION     DAOTMX010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26015_length_2972_cov_2.658210"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1778)
                     /locus_tag="P5573_06675"
     CDS             complement(<1..1778)
                     /locus_tag="P5573_06675"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HRT27779.1"
                     /translation="MLRITDILDKVQGYMSSEEIEMIEKAYIFSASVHQGQVRLSGEP
                     YLTHPMEVAGILADMKLDCATIVTALLHDTVEDTFTTLEQIEESFGKEVAFIVDGLTK
                     ISRITFGSQEERQAENFRKMMLAMSSDIRIILVRLADRVHNMRTLEYQQPERREYIAK
                     ETLEIYAPLANRLGINWMKTELEDLSFRHLYPEAYRDLAARIDMRREERDRYTEEIKE
                     IIRKQVAEYGLKAEVEGRAKHFYSIYKKMKEQHIDFDEVYDLMAFRIILDSNKEKECY
                     EALSVVHALWKPVPGRFKDYIAMPKANNYRSLHTTVIGPYGERVEIQIRTREMHEWAE
                     EGIAAHWRYKEKGAIPGRDELQIKKLRELLDFQQESKDPREFMKNLKLALFPDEVYVF
                     TPAGDVKAFPKGATPIDFAYSIHTDVGDQCVGAKVNRTIVPLKYELQNGDTVEIITQA
                     GHHPSKDWLKYVVTSKALSKIKNWIKTEERRKSVALGKDILEKEFRKHQLKFNQVVKL
                     EEFKKLCAELSVNSPDDLLAIVGYGKVSPKHIVNRFVEDEEKKREEEETILDKLKKRF
                     SRPSAAGVSITGVEDVMVRFSKCCDPL"
     gene            complement(1784..>2972)
                     /gene="proS"
                     /locus_tag="P5573_06680"
     CDS             complement(1784..>2972)
                     /gene="proS"
                     /locus_tag="P5573_06680"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041584864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HRT27780.1"
                     /translation="KMYDAYSQIFTRCGLKFRAVEADTGNIGGSFSHEFMAIAETGED
                     AMVFCSNCDYAANIEKAEVARPERLPVDEAELKPLESVHTPGVRTIEEVCSFFGVTPD
                     HIVKTLIFVADGKPVAVLIRGDEEVNEAKLRTHLNCDELELATDDVVLEVTGSPKGFA
                     GALGIKSPVIADYSLINMKNFIMGANREDHHVVNANIGRDFKVETFADLRIIKEDDLC
                     PRCGKPIRFARGIELGHTFKLGTKYSKAMRATFLDRDGKELEMVMGCYGIGIGRTVAA
                     SIEQNNDAKGIIWPISIAPYHVIVTPVNVKDGAVMEAAEKLYSRMLEASVEVILDDRD
                     ERAGVKFNDADLIGIPIRFTVGPKRVAEGKVEVRMRKTGEEKVIDLDKAVDFVRPMVE
                     EAT"
BASE COUNT          527 a          892 c          798 g          755 t
ORIGIN      
        1 agggggtcac agcacttgga aaaccgcacc atgacgtctt cgacgcccgt gatggacacc
       61 ccggcggcgg agggccgcga aaagcgtttc ttcagcttgt cgaggatggt ttcctcttct
      121 tctctcttct tctcctcgtc ctcgacgaag cggtttacga tgtgcttcgg ggagaccttc
      181 ccgtaaccta ctatcgccag caggtcgtcg ggggagttga cggagagctc ggcgcacaat
      241 ttcttgaact cttcgagttt aacgacctga ttgaacttga gctgatgttt gcggaactcc
      301 ttctccagga tgtctttacc gagggcgacg ctcttcctcc gctcttccgt cttgatccag
      361 ttttttatct tcgacagggc cttcgaagtg acgacgtact tcagccaatc cttgctcggg
      421 tggtggcccg cctgggtgat gatctccacc gtgtccccgt tttggagctc atacttgagc
      481 ggcacgatcg tcctgtttac cttcgccccc acgcactggt cgccgacgtc cgtgtggatg
      541 ctgtaagcga agtcgatggg tgtggcgccc tttgggaagg ccttcacatc gcccgccggc
      601 gtgaagacgt atacctcgtc ggggaagagg gcgagcttta ggttcttcat gaattctcgg
      661 gggtccttcg attcctgctg gaaatcgagg agttcccgga gcttcttgat ctggagctcg
      721 tcgcgtcccg gtatggcgcc cttttctttg taacgccaat gggccgcgat cccctcctcg
      781 gcccattcgt gcatctcacg cgtcctgatc tggatttcga cgcgctcccc gtagggcccg
      841 atgaccgtcg tatggaggga acggtagttg ttggccttgg gcatcgcgat gtagtccttg
      901 aaacgtccgg gcacgggttt ccagagggcg tggacgacgc tcagggcctc gtaacactcc
      961 ttttccttgt tcgaatcaag gatgatcctg aaggccatca ggtcgtagac ctcgtcgaag
     1021 tcgatgtgct gctccttcat tttcttgtag atactgtaga agtgcttcgc cctaccctcg
     1081 acttcagcct tgaggccgta ttcggcgacc tgcttacgga tgatctcttt gatctcctcc
     1141 gtgtaccggt cgcgctcctc gcgcctcatg tcgatccgcg ccgccaggtc acggtaagcc
     1201 tcggggtaaa gatgtctaaa ggaaaggtcc tcgagttccg tcttcatcca gttgatgccg
     1261 agcctgttcg cgaggggggc gtatatttcg agcgtttcct tggcgatgta ttcccttctt
     1321 tccggctgct ggtattcgag cgtcctcata ttgtggaccc ggtcggcgag acggacgagg
     1381 atgatcctga tgtcggacga catggccagc atcatcttgc ggaagttttc cgcctggcgt
     1441 tcctcctgac ttccgaaggt gatcctgctg atcttggtca gaccgtccac gatgaaggcc
     1501 acttcttttc cgaaggactc ctcgatctgc tccaacgtcg tgaatgtgtc ttccaccgtg
     1561 tcatggagaa gggccgtcac gatcgtcgcg cagtcgagct tcatgtccgc caagataccg
     1621 gcaacctcca tcgggtgcgt cagatagggc tcaccggaga gccggacctg gccctggtgc
     1681 acagaggccg agaagatgta agccttctct atcatctcta tctcttccga tgacatgtag
     1741 ccctgcacct tgtcgaggat gtccgttatc cgaagcatac gtcctacgtg gcttcttcta
     1801 ccataggcct gacgaaatcg actgccttgt ccaggtcgat gaccttttct tcgcccgtct
     1861 tccgcatccg gacctcgacc ttcccctcgg ccaccctctt tgggccgacc gtaaaccgga
     1921 tcggtatacc tattaggtcc gcgtcgttaa actttacgcc cgccctttcg tccctgtcgt
     1981 cgagtatcac ctcgacggat gcctcgagca tccgggagta gagtttttcg gcggcctcca
     2041 tgaccgcgcc gtccttgaca ttcacgggcg tcacgatgac gtggtacggg gcgatcgata
     2101 tcggccagat gatgccttta gcgtcgttgt tctgttcgat cgatgccgct accgtacggc
     2161 caataccgat gccataacaa cccatgacca tctccagctc cttcccatca cggtcgagga
     2221 aggtcgccct catcgccttg ctgtatttcg tcccgagctt gaatgtatgg ccgagttcta
     2281 tgcccctggc gaagcggata ggcttgccgc accgcgggca caagtcgtcc tctttaatta
     2341 tacgcaagtc ggcgaaggtt tcgactttga aatcacggcc gatgttggcg ttcacgacgt
     2401 ggtggtcctc tctgttcgca cccatgatga agttcttcat gttgatgagc gaataatcgg
     2461 cgatgacagg ggattttatt cccagagcac cggcgaaacc cttgggtgaa cccgtgactt
     2521 cgagaaccac gtcgtctgtc gccagttcga gctcatcgca atttaggtgc gtcctgagct
     2581 tggcctcgtt cacctcctcg tcgcccctta tcaggacggc gaccggcttt ccgtcggcga
     2641 caaagatgag ggtctttacg atgtggtccg gcgtcacacc gaagaaagag catacctcct
     2701 ctatcgtcct caccccgggc gtgtggaccg attcgagggg ctttaattcc gcctcatcca
     2761 ccggcaacct ctccggcctc gcaacctctg ccttttcgat attcgccgca tagtcgcagt
     2821 tactacagaa taccatggcg tcttcgccag tctcggcgat ggccatgaac tcgtgggaaa
     2881 aactaccgcc gatattgccc gtgtcggcct caacggccct gaacttcaaa ccacagcgag
     2941 tgaatatctg cgagtaggca tcgtacatct tc
//
LOCUS       DAOTMX010000221         2967 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_26059_length_2967_cov_4.227679, whole genome shotgun sequence.
ACCESSION   DAOTMX010000221 DAOTMX010000000
VERSION     DAOTMX010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26059_length_2967_cov_4.227679"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..1120
                     /locus_tag="P5573_06685"
     CDS             83..1120
                     /locus_tag="P5573_06685"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HRT27781.1"
                     /translation="MRVAMYYNNNDVRLEEMPIPAIGRGEILVKVIASGICGSDVLEW
                     YRVRKAPLVLGHEIAGEIVEVGEGVKGWRTGDRVFVSHHIPCNTCRYCLYGHHTACET
                     LHTTNYDPGGFAEFIRVPALNVDRGVFLLPDELSFDQGAFVEPLACVVRGQRVAGLQP
                     GRCVLVLGSGISGILHILLARALGAGRIISTDVHDYRTGKAREFGADAAFRADDDIPE
                     RVRDANGGRLADLVIVCTGALSAFHQAMQCVDRGGTVLGFATTEPDVRLPVPINEFWR
                     NEIRLMPSYGNAPLDAIEAIELLRAGRVSVDGMVTHRLGLEETGLGFRLTASAGESLK
                     VIVEPHRPPRG"
     gene            1250..2254
                     /gene="obgE"
                     /locus_tag="P5573_06690"
     CDS             1250..2254
                     /gene="obgE"
                     /locus_tag="P5573_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HRT27782.1"
                     /translation="MKFIDEAKIYVKAGDGGRGCVSFRREKYVPKGGPDGGDGGDGGD
                     VVVTAAAGCRTLLDLKFHQHHVAKRGGHGSGNNRRGRDAGDVVVTVPIGTVITDAETG
                     EVLGDLTKDGERVVVARGGIGGKGNAFFATATNQAPRFAQEGTTGEERWLNLELKLLA
                     DVGIIGLPNAGKSTLISRISAARPKIADYPFTTLVPNLGVVKYGNAEPFVVADIPGLV
                     EGAHRGTGLGMRFLRHIERTSVLVHLIDLSRQDEGDARKDYEVIRRELGRFSDNLIQK
                     PEVVAFNKVDVPAARERVKKEIDRFREKGIETFAVSAATGEGLETLLQAIIKILRRSN
                     "
     gene            2296..>2967
                     /gene="proB"
                     /locus_tag="P5573_06695"
     CDS             2296..>2967
                     /gene="proB"
                     /locus_tag="P5573_06695"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HRT27783.1"
                     /translation="MAGVKKVLIKVGTGVLTGKDGLDLTIIDNLVEDIAEITRKGCRV
                     VIVTSGAIASGKHRMGITGKLKSMPEKQAAAAIGQSRLMRVYSNAFGKHNLYVAQILL
                     TMSDLTDRARYINVRNTLSQLMEWGVIPIINENDTVAVDELKFGDNDHLAAMIANVVE
                     ADVLINLTNTEGLYDRNPKDKKRAKLIRIVREITEDIEAAATAEADPVGMGGMRSKVV
                     AAKKVT"
BASE COUNT          653 a          897 c          924 g          493 t
ORIGIN      
        1 catccacgaa aacgcctcgg cgaaagaagc ccacaagctt ttcaccagtt tgaagaaatc
       61 gacctgattc ccggagacgg cgatgcgcgt cgccatgtat tacaacaaca acgatgtgcg
      121 actcgaagag atgcccatcc cggcgatagg ccgcggtgag atactggtaa aggtcatcgc
      181 gagcggcatc tgcgggagcg atgtcctgga gtggtaccgc gtccggaagg cgcccctcgt
      241 cctgggacat gagatagccg gcgaaatcgt cgaagtgggc gaaggcgtga aaggctggag
      301 gaccggcgac cgtgtcttcg tttcccacca catcccctgt aatacctgtc gttactgctt
      361 gtacggccat cacacggcct gcgaaacgct ccacacgacg aattacgacc cgggtggatt
      421 cgccgaattc attcgggtac cggccttgaa cgtcgaccgc ggggtctttc tcctccccga
      481 tgagctctct ttcgatcagg gtgccttcgt cgagcccctc gcctgcgtcg tccgcgggca
      541 gagggtcgcc gggcttcaac cgggccggtg cgtcctcgtc ctgggaagcg gcatctccgg
      601 catactgcac atcctgcttg cccgggccct cggcgcaggc cggataatct ccacagacgt
      661 ccatgattac cgcaccggaa aggcaaggga attcggtgcc gacgccgcgt tccgcgccga
      721 cgacgacata ccggaacgcg tccgcgacgc aaacggcgga aggctcgccg acctcgtcat
      781 cgtgtgtacc ggcgccctct ctgccttcca ccaggccatg cagtgcgtgg accgcggggg
      841 gacggtcctg ggctttgcca cgacggaacc ggacgtccgg cttccggtcc cgataaacga
      901 attctggagg aacgaaatca gactcatgcc ttcctacgga aacgcgcccc tcgacgccat
      961 cgaggccatc gagctcctgc gggccgggcg ggtctccgtc gacgggatgg tcacccaccg
     1021 gctgggcctc gaggaaaccg ggttgggctt cagactgact gcctcggccg gagaatccct
     1081 caaagtcatc gtcgaacctc atcgaccccc gcggggatga ccccgcatac cttgtaatcc
     1141 cacaataaat ccggtaatct aaagtatatt taattggtta agaatgttcg ggcttaattt
     1201 ttcggggggg cgtcaatgcc ccgttgcccg tcaggggacg gactgtcata tgaaatttat
     1261 cgacgaagcg aaaatctacg tcaaggccgg cgacggcggg aggggttgcg tcagcttccg
     1321 gagggagaag tacgtcccca agggcggtcc cgacggggga gacggaggcg acgggggcga
     1381 cgtggtcgtc acggcggcgg ccggctgccg gacgctcctc gacctcaagt tccaccagca
     1441 tcacgtcgcg aagcgcggcg gccacggaag cggcaacaat cgccggggca gggacgccgg
     1501 cgacgtggtt gtcaccgttc ccatagggac ggtgatcact gacgccgaga caggggaggt
     1561 cttgggggac ctcacgaaag acggcgaaag ggtcgttgtc gccagggggg gtatcggggg
     1621 gaaagggaat gccttcttcg ctacggcgac caaccaggcc ccgcggttcg cccaggaagg
     1681 gacgacggga gaagagcggt ggctaaacct ggagctcaag ctcctggctg atgtgggcat
     1741 catcggtctg ccgaacgcgg gcaagtccac cctgatctcc agaatctccg ccgcccggcc
     1801 caagatcgcc gactatccct tcacgacact ggtcccgaac ctgggcgtgg tcaagtacgg
     1861 gaacgcggag cccttcgtcg tcgccgacat ccccggattg gtcgaggggg cgcaccgggg
     1921 gaccggcctg gggatgcggt ttctgcgcca catcgagcgc acgtccgtcc tggtccatct
     1981 catcgacctt tcccggcagg acgaaggcga cgcccggaaa gattatgaag tcataaggcg
     2041 cgagctcggt cggttcagcg acaatttaat ccagaaaccc gaggtcgtgg ccttcaataa
     2101 ggtcgacgtc cccgccgcgc gggaaagggt gaaaaaagaa attgacagat tcagggaaaa
     2161 gggcatagaa accttcgccg tttcggccgc cacgggcgag ggattggaaa ccctactgca
     2221 agcgataatc aagatacttc ggcgttcgaa ctgagaaagg aataactacc gatgtcggat
     2281 ctaagaaaag atataatggc cggcgtaaaa aaagtcctca tcaaggtggg gacgggggtc
     2341 ctgacgggca aagacgggct ggacctcacc ataatcgaca acctcgtcga ggacatcgcc
     2401 gagatcaccc gaaagggctg ccgcgtcgtg atcgtcacct ccggggccat cgcctcgggc
     2461 aagcaccgga tgggaatcac gggcaaactc aagagcatgc cggagaaaca ggctgcggcg
     2521 gctatcggcc agagccgcct catgcgcgtc tactccaacg ccttcggaaa gcataacctc
     2581 tacgtcgctc agatccttct caccatgagc gacctcaccg accgtgcaag gtacataaac
     2641 gtccgcaaca ccctctcgca gctgatggag tggggtgtca tcccgatcat caacgaaaac
     2701 gacaccgtcg ccgtcgacga actcaagttc ggcgacaacg accatctcgc cgccatgata
     2761 gccaacgtcg tcgaggcgga cgttttgatc aacctcacca acactgaagg tctctacgac
     2821 cggaacccca aggacaagaa gcgggcaaag ctgatccgga tcgtccggga gatcacggag
     2881 gacatagaag ctgcggccac agcggaagcg gaccccgtgg ggatgggcgg gatgaggagc
     2941 aaggtggtcg ccgcgaaaaa ggtgacg
//
LOCUS       DAOTMX010000222         2948 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_26266_length_2948_cov_4.277221, whole genome shotgun sequence.
ACCESSION   DAOTMX010000222 DAOTMX010000000
VERSION     DAOTMX010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26266_length_2948_cov_4.277221"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            106..1320
                     /locus_tag="P5573_06700"
     CDS             106..1320
                     /locus_tag="P5573_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27784.1"
                     /translation="MFGGFVISIKGYLYRDEFRDLIRRAMSEVVRENDGVRLTRLVLF
                     NQLYVARYLNRFARELFESLHGGPLRQEMIFTKGRLKDAIVERPPYVNERIAGLISDY
                     KDYPGRFYRETPCRAMLFFKSRGGCDGEDFIGSWRIKRIRRLAEKAARKIIDWIFDAI
                     KGQAERLAEERAARLSIPRQYLISSPEEMLAEFLDAEKRFIEYLREHREVDVAQDLAI
                     NDVAGIKVLLEDDEKDGLLRVLEGIEGCKVLEEERHTGRYNATNLLIRLRPTKEEMIQ
                     GPLLPDVVCAMEKKGWKAGEVERCFADFVMTGEEEVYLEIIVSNYEQLLESEIGRCMH
                     EDRIIAQRLHRQYRGHLAKNVEYLMRFLFLFPLSDREELLQLPIRLWDRYLPDYFDSV
                     IADLFHIPQGDF"
     gene            1375..2787
                     /locus_tag="P5573_06705"
     CDS             1375..2787
                     /locus_tag="P5573_06705"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HRT27785.1"
                     /translation="MNDVIRRMSALFDPQSIAFVGATNRSDKWGFRILSGLLHYGYRG
                     KVYPINPSEHEDILGIKPFPSVSSIPESPDLAVVVTPQPTVLGIVGECVQKGVKACLV
                     ITAGFAELGRKEEERLENEIVDTARKGGMVLVGPNSNGIMSMPSNLYLLMSRRLERTK
                     HGSLSITSRSGSVGTSVLALCVDRNFGFNRFVSNGNEADLHIEDYLAFYGEDETTKVI
                     LGYTEGIRDGERLIDIARGVNRKKPVVMLMAGSTDAGARAARYHTASSSGGSELLYQR
                     ACREAGIIRVDDIEEIFTIGASFIRQPLPKGRRVGILTIGGGWGVVASDACTRAGLDV
                     TELPEETLKRMDNYLPWWWSRNNPVDTGASLEWRGCLEALAECQDVDMILVMWVAGKE
                     MTPATELCPVMESLIEQHHKPIILCSGGFETREAVEEFGKRQLVAFHSIHQAVKALSS
                     LAKYSEYLSGGLSLAQVPKG"
BASE COUNT          690 a          740 c          884 g          634 t
ORIGIN      
        1 ccgttccatg aaacagatat tgccgggata cagaattgtg gtcttgacgt ggcccttttt
       61 gtggaataga ttttcagctt ccaagggata taaaaccatt taccggtgtt cggagggttc
      121 gtgatctcca tcaagggata cctataccgg gatgagttcc gagacctcat ccggcgggcc
      181 atgtcggagg tcgttaggga aaacgacggt gtaaggctca cccgactagt cctcttcaac
      241 cagctttacg tcgcccgtta tctgaacagg tttgcgcgag aactcttcga gtccttgcac
      301 ggcggcccct tgcggcagga gatgatcttc actaagggaa ggcttaaaga cgccatagtc
      361 gaacggccgc cctacgtaaa cgaacggata gccggtctga tatccgacta caaggattac
      421 cccggccgtt tttacaggga gacgccctgc cgggcgatgc tgtttttcaa gagccggggg
      481 ggatgcgatg gagaagactt catagggtcc tggaggatca agcggatcag gaggttggcg
      541 gaaaaggcgg ccagaaaaat aatcgattgg attttcgacg ccatcaaggg gcaagcggaa
      601 cgtcttgcgg aagagcgggc cgcccgccta tcgataccac gccagtacct tatatcatca
      661 ccggaggaga tgctcgcgga gtttctcgac gccgagaagc gtttcatcga gtacctgagg
      721 gagcatcgag aagtggatgt ggcgcaggac cttgctatca acgacgtcgc cgggataaag
      781 gtgcttttgg aggatgacga aaaagacggc ctcctcaggg ttttggaggg catagaagga
      841 tgcaaggtat tggaagagga gcgccacacg ggacggtaca acgcgacaaa cctattgatc
      901 cgtctgcggc cgacgaaaga agagatgatt cagggaccac ttctgccgga cgtcgtctgc
      961 gccatggaaa agaagggatg gaaggcgggg gaggttgagc gctgctttgc cgattttgtc
     1021 atgacagggg aggaggaagt ttatctcgag atcatagtct ccaattatga gcagttgttg
     1081 gagagcgaga tcgggcgctg tatgcacgag gaccgcatca tcgcccagcg tctccaccgt
     1141 cagtaccggg ggcacctggc aaagaacgtc gaatatctca tgcgttttct cttccttttc
     1201 cctctgtccg accgggagga attgctgcaa cttccgatca ggctctggga ccgttaccta
     1261 ccggactatt ttgacagcgt catcgcagac ctcttccaca tcccccaggg agatttttaa
     1321 gcgaggcgcc gggatggaac atagaatcgg aactgtaagg aggtctttgg agagatgaac
     1381 gatgtaatcc gtagaatgag cgcccttttc gacccacaga gtatcgcctt tgtaggagcg
     1441 accaaccgga gtgacaaatg ggggtttcgt atcctgtccg gcctccttca ttacggatac
     1501 cggggtaagg tttacccgat aaatccttcg gagcacgaag atatcctggg cataaagccg
     1561 tttcctagtg tttcgtccat ccccgagtcg cccgacctcg ccgtcgtcgt cacccctcag
     1621 ccgacggtac tcggcatcgt cggggaatgc gtccagaagg gtgtaaaggc ttgtctcgtc
     1681 ataaccgccg ggtttgccga actcgggcgt aaggaggaag agcggttgga gaatgagata
     1741 gtcgacacgg cgagaaaagg gggcatggtg ctggtcggtc ccaacagcaa cggcatcatg
     1801 agcatgccct cgaatctgta cctcctcatg tcgaggcggc ttgaaaggac caagcatggg
     1861 tcgctgtcga tcacgtcccg gagcggcagc gtagggacat ccgtgctggc gttgtgcgtc
     1921 gacaggaatt ttgggttcaa ccgctttgtc tccaacggga acgaagccga ccttcacata
     1981 gaggattatc ttgcctttta tggggaggat gaaacgacta aagtcatcct cggttacacc
     2041 gagggcatac gggatggcga acgattaatc gatattgcgc gaggagtgaa ccggaaaaag
     2101 cccgttgtca tgctcatggc cggttctacc gatgcggggg cgagggccgc ccgttaccac
     2161 accgcgtcat cctccggggg ctccgaactc ctctatcaga gggcctgccg ggaggcgggc
     2221 ataataaggg tcgatgatat cgaagaaata ttcaccatcg gggcttcgtt catccgccag
     2281 cccttgccga agggcaggag agtgggcatc ctcactatag gcggcgggtg gggggtcgtc
     2341 gccagcgacg cctgtacccg ggcggggttg gacgtgaccg agcttcccga ggagacactg
     2401 aaaagaatgg acaactatct gccgtggtgg tggagccgta acaaccccgt cgacacgggg
     2461 gcttcactcg aatggcgcgg ctgtcttgag gcattggccg agtgtcagga cgtcgatatg
     2521 atcctggtga tgtgggtcgc cggcaaagag atgacaccgg cgactgagct gtgtcccgtc
     2581 atggagagtc tgatcgagca gcatcataaa cccataatcc tctgctccgg tggtttcgag
     2641 acccgtgagg ccgtagaaga attcgggaaa agacaactcg tcgcctttca ttccatccac
     2701 caggccgtga aggccctgtc gagcctcgcg aaatattccg agtacctgtc cggtggtctt
     2761 agccttgctc aagtacccaa agggtgatgt tcatttcgac accaggatga gcatagggca
     2821 ggccactact gtcccaactt ttatttttga aagtgtgata acatcataaa atataaagaa
     2881 ttctcacgaa aaatcagcaa tgtccggtta aggttttgca tgtaaggtta tagatcacca
     2941 agggcttc
//
LOCUS       DAOTMX010000223         2944 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_26322_length_2944_cov_3.076497, whole genome shotgun sequence.
ACCESSION   DAOTMX010000223 DAOTMX010000000
VERSION     DAOTMX010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26322_length_2944_cov_3.076497"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            395..1699
                     /locus_tag="P5573_06710"
     CDS             395..1699
                     /locus_tag="P5573_06710"
                     /inference="COORDINATES: protein motif:HMM:NF015056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HRT27786.1"
                     /translation="MKLPKTILVNTFTGGLVGPSIPMVGPVANGGTIIAETAPGCWGP
                     MITPSFKGGHEVTSPVAVEGAVPGDAIAIRIKKVRVTSVVTASGTMGFVEGRYTGDPF
                     VSRHCPNCGTISPLTRIEGVGADAVRCANCGAEVSPFRLTNGYTIVFDERRKIAVTVN
                     GRVARKLAKDAAKLMALPESSCQNPVVALAPADISGLTTRLRPFVGNIGTTPAIDMPD
                     SHNAGDFGQFLLGAPHEYGITKEQLENRTDGHLDIDSVREGAILICPVKVPGGGVYVG
                     DAHAMQGDGEIAGHTTDVAAKVTLQVNVVKGLKIGGPILLPPVKDLPHLARPFTAAEK
                     KEADALAKKFGQTALEDALPIQMVGSGANLNEAADNGVARLAELFQISREEVMNRVTL
                     SGAVEIGRLPGMVAVTMRVPLPWLKKVGLIKLVKEQYSSEKP"
     gene            complement(1739..2584)
                     /locus_tag="P5573_06715"
     CDS             complement(1739..2584)
                     /locus_tag="P5573_06715"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRT27787.1"
                     /translation="MNYTLTSTVDLPGGVKMPILGLGTFLSEPGSTARQAVMWALEAG
                     YRHIDTARIYNNERDVGEGVRESGLQRAEVFITTKVWNDDQGYDSTLRACEESLKRLG
                     MDYADLYLIHWPVPQKRLETWKALIRLREEKTVRAIGVSNFMVRHLDELLGTSDVVPA
                     VNQVEISPFIQRKALVDYCRAKGIVVEAYSPLSRGQRLADPRLAAMAKKYGKTPAQIA
                     LRWVVQRGIVAIPKSVHRERIVENAAIFDFVIDEGDMAEIARWDEHYSVLFASWEPET
                     SNLWD"
BASE COUNT          688 a          829 c          800 g          627 t
ORIGIN      
        1 cgacaaggac ctggacgata ttcaggatat ggtgaacgag gactgggagc tgctcctccg
       61 caaggccgac ttgcacgcct gaggaccttt cgtccccggt tagtgggacg cgccggcatt
      121 ccaccatggc ccttagccgt tataggcaga agtgtaggcc cttactttct ctaccccatg
      181 ttccatcagc ttccgggctc cggtgtgcgg ctttttccac tgtcgccaca ggacgtaaat
      241 gttgcagtca tcggcataac ggcggaaacg atagggacaa aaatactaac cagagaacaa
      301 gtgacataaa aatgatctta agcttattcg attgacaatc gatgcctgct tttctaaggt
      361 ctttagtcaa accaacaaca aggagggaat cgtcatgaaa ctgccaaaaa caatcctcgt
      421 gaacacgttt accggcggtc tggtggggcc gagcattccg atggtcggtc cggtggctaa
      481 cggcggcacg ataatagcgg aaactgctcc gggatgttgg ggtccgatga taaccccttc
      541 cttcaaggga ggtcacgagg tcacaagtcc tgtggccgtt gagggtgccg taccaggaga
      601 tgcgatcgct atccgcatca aaaaagtcag agtaacgtcg gttgtcactg catccggcac
      661 catgggtttt gttgaggggc gttacacggg cgatcctttc gtgtcccgac attgccccaa
      721 ttgcgggaca ataagccccc tcacacgtat cgaaggcgtc ggggcggacg ccgtgcgctg
      781 tgccaattgc ggggccgagg tcagcccctt ccgtcttact aacggctaca caatagtctt
      841 cgatgagagg cgcaaaattg ccgtcaccgt aaacggtaga gtagccagga aacttgccaa
      901 agatgccgcg aagctcatgg ccctacccga atcttcgtgc cagaaccctg tcgtagccct
      961 ggccccggcg gacatctccg gtttaacgac ccgccttcgg ccgttcgtcg gtaatatcgg
     1021 caccactcct gctatcgata tgcccgactc tcacaatgcc ggtgatttcg gacagtttct
     1081 cctgggcgct ccccatgagt acggcatcac taaagaacaa ttggaaaacc gtacagacgg
     1141 tcatctggac atcgattccg tgagagaagg ggcgattctc atctgccccg tcaaggtgcc
     1201 gggcggtgga gtttatgtgg gcgatgccca cgcgatgcag ggagacgggg aaatagcggg
     1261 acacacgacg gacgtggccg cgaaagtaac actacaggtg aacgtcgtca agggcttgaa
     1321 aatcggtgga ccgatactcc tgccgcccgt aaaagacttg ccccatctcg ccagaccttt
     1381 cactgcggcc gaaaaaaaag aggcggacgc cctggctaag aaattcggac aaacggcact
     1441 tgaggatgcg cttccgatac agatggtcgg ttccggggcg aacttgaatg aagcagccga
     1501 caacggtgtg gcacgcctgg cagagttatt ccagatttcc cgggaagagg ttatgaatcg
     1561 agtcaccctc tcgggcgcgg tcgagatcgg acggctgccg ggaatggtcg cagtgaccat
     1621 gagggtacct ctgccctggc tgaagaaggt aggcctgata aagctggtca aagaacagta
     1681 ctcctccgaa aaaccttaga cggttcatag aaccggaaat cgagcgcact tccaagattc
     1741 agtcccagag gttcgacgtt tccggttccc aggaggcgaa caagacggag taatgctcat
     1801 cccagcgggc gatctcggcc atgtccccct catcgatcac gaaatcgaag atcgcggcgt
     1861 tttcgacgat cctttctcga tgcacggatt tgggaatggc cacgattccc cgctggacca
     1921 cccaacgcag ggcaatctga gccggtgttt tcccgtattt cttcgccatc gccgccaagc
     1981 gcgggtccgc cagccgctga ccacggctta agggactgta agcctcgacc acgataccct
     2041 tcgcacggca atagtcgaca agcgctttac gctggatgaa agggcttatc tcaacctggt
     2101 tgacggcggg gacgacatcg gaggtgccaa gtagttcgtc caaatgacgg accatgaaat
     2161 tgcttacacc gatcgcccgg accgtctttt cttcccggag acggatgagc gccttccagg
     2221 tctcgagtct tttttggggc acgggccagt gaatgaggta caggtccgcg tagtccatcc
     2281 ccagtcgctt gaggctttcc tcgcaggccc tgagggttga atcgtatccc tgatcgtcat
     2341 tccatacctt cgtcgtgatg aatacctccg cgcgctgaag gccgctctcc ctcacccctt
     2401 caccgacatc cctttcattg ttgtagatac gggccgtatc gatatggcgg tagcccgctt
     2461 ccaacgccca cataaccgct tgacgtgccg tcgatccggg ctcggaaagg aaggttccga
     2521 gtcccaggat gggcatcttg accccacccg gcaaatcgac agtcgatgtc agtgtgtaat
     2581 tcattttcac cctccgtttt tatttgcttt acgcgaatcc tcatcctcct gcgttagacc
     2641 ttgtcaacct acctgaaccg cataaagata ttgactttta cgccgggcaa gattagagca
     2701 tgaaacgtta ccatccagaa tgtccgcggc catggccaca ataacaatag atgccggggc
     2761 aatcaagaca cttgactgca tgatagggaa aatttttctc tgacggcagg gagctgtagt
     2821 aacggcgcga atattgctta aaattggcta agattgaacg ctccctgcaa aaagaaccgg
     2881 aaggaaattc ggctgccttt ccccgggcgc tcggcaacaa gccacggcga ggctcaacaa
     2941 aatc
//
LOCUS       DAOTMX010000224         2941 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_26359_length_2941_cov_2.603950, whole genome shotgun sequence.
ACCESSION   DAOTMX010000224 DAOTMX010000000
VERSION     DAOTMX010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26359_length_2941_cov_2.603950"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="P5573_06720"
     CDS             complement(<1..851)
                     /locus_tag="P5573_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(264..266),aa:Sec)
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRT27788.1"
                     /translation="MKISRRGFLTLSGAVGSGLALSSLGINLKSLRAYADELDKMNKV
                     KSARQVTTICCYCSVGCGLVCSVDNATGKIFNIEGDADHPINEGSLCAKGAGLFDLTE
                     ANKYRLKKVLYRAPMSDKWEEKNWEWALNRIARLVKDTRDSGFVTRNSKGELVNRCET
                     IGHLGSSNVDNEECWLMSLKARALGLVYIDHQARVUHSPSVAALGESFGRGSMTNHYI
                     DLKNSDVILIMGSNAAEHHPIAFKWILRAKEQNGARLIHVDPRYTRTSARCDYHVPLR
                     SGTDIAFL"
     gene            complement(1056..1937)
                     /locus_tag="P5573_06725"
     CDS             complement(1056..1937)
                     /locus_tag="P5573_06725"
                     /inference="COORDINATES: protein motif:HMM:NF016131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HRT27789.1"
                     /translation="MKQANDSLKSLEKTIDRVIETCPHSGEILDAFRPVILERHRLME
                     NMGWEPVDTAVIDRKKLNKGIPLIRQVELLRRGDPWKEVALALIPAFKQGFTDLQGDL
                     DALQDCIMSGDLDFYEYFRASSGDADRIPERLSEKANVRPAASRLFLRTLTWVVLHRR
                     RRDMAVSIDEADWHQGTCPVCGSFPSISVITEKNAQRWLHCFQCGQDWRFSRVICPYC
                     AHEGQRGMTFFFVEDRKQECVFICEQCNRYLLTLNQVRDLQEIDLDISALGLAHLDIL
                     MRDKGYVPMVPAEWNPS"
     gene            complement(2154..2693)
                     /locus_tag="P5573_06730"
     CDS             complement(2154..2693)
                     /locus_tag="P5573_06730"
                     /inference="COORDINATES: protein motif:HMM:NF021561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2284 domain-containing protein"
                     /protein_id="HRT27790.1"
                     /translation="MQLSDLDKYCEKAIQAGATDAKVIHPSTVVTAPWVRFKCLFGCP
                     YKHRYSCPPHTPTPAETQALLDSYRRAILFHVDAPYSKERARNMVAYMDALVKLEGEL
                     FKDGYYKAFLMLAGPCVLCKECGLNEGIPCRFPAKTRPSMESCGIDVFQTARNNGFFI
                     KTLKEKSETQNLYCLMLVD"
BASE COUNT          590 a          838 c          815 g          698 t
ORIGIN      
        1 aggaaggcga tgtccgtccc ggagcggagg gggacatggt aatcgcagcg ggccgatgtc
       61 cgggtgtaac gggggtccac gtggatcagt cgggccccgt tctgctcttt ggccctcaaa
      121 atccacttga aggcgatggg gtgatgctcc gcagcgttgc ttcccatgat caggatgaca
      181 tcgctgtttt tcaaatctat gtaatggttc gtcatcgaac cgcgtccgaa cgactctccc
      241 agagccgcta cagaggggct gtgtcagacc ctggcctggt ggtcgatgta gacgaggcca
      301 agggcccttg ccttgaggct catcaaccag cactcttcgt tgtccacatt ggaactgccg
      361 aggtgaccga tcgtctcaca gcggttgacg agctcgccct tgctgttgcg cgtgacgaaa
      421 ccgctgtccc gcgtgtcctt gacaaggcgg gcgattcgat taagtgccca ttcccagttc
      481 ttttcctccc acttgtcgct catgggggcc cggtagagga ccttcttgag ccgatatttg
      541 tttgcctccg tcaagtcgaa gaggccggcg cctttcgcgc agagcgaccc ctcgttgatg
      601 gggtggtccg cgtcgccctc gatgttgaaa atcttgcccg tagcattgtc cacgctgcag
      661 acgagaccgc agccgacgga gcagtaacaa caaatcgtgg tcacctgccg ggcgctcttg
      721 accttgttca ttttgtcgag ttcatccgca tacgcccgca gagacttcag gtttatgccc
      781 agggaagaca gggccaaccc ggaacctaca gcgccggaca gggtcaggaa ccctcttctg
      841 cttatcttca tacttaggag cctcctatcc tgctcatgtc aaacttgagc tatggtacgt
      901 ttgcgcatat ctaatttctt ttctccgata agtcaacaga aatgtggttc ctaaggttgt
      961 gttattatta acataactcc gtgtaaagct gtgggttctg acgtgccggg aaggtcatga
     1021 ggaggggctt cggctggggt tcggtcagcg gtgtcctacg acgggttcca ttcggccggc
     1081 accatcggga catagccctt atccctcatc aggatgtcga gatgagcgag gccgagggct
     1141 gagatgtcga ggtcgatctc ctggaggtcg cgcacctggt tgagtgtgag caggtaccgg
     1201 ttgcattgtt cgcagatgaa gacacattcc tgctttctgt cctcgacgaa aaaaaaggtc
     1261 attccccgtt gaccttcatg cgcgcaatag gggcagatga ccctgctgaa gcgccaatcc
     1321 tggccgcatt ggaagcagtg aagccaccgc tgggcatttt tttccgttat cacggagatc
     1381 gacgggaagg aaccgcatac cggacaggtg ccctggtgcc agtcggcttc gtctatggag
     1441 accgccatgt cgcgcctcct cctgtgaaga acgacccacg tcagcgttct gagaaacagg
     1501 cgggaagcgg cgggtcggac gttcgccttt tcggacaaac gttccgggat tctatcggcg
     1561 tcgccggatg atgccctgaa atattcgtaa aaatcgaggt caccgctcat gatgcagtcc
     1621 tgcaacgcgt ccaggtcacc ctgaaggtct gtaaaaccct gcttgaaagc cgggatcaag
     1681 gcaagggcga cctctttcca ggggtcgccg cgccgcaaca gctccacctg tcttatcaag
     1741 ggaattcctt tattcaattt tttccggtcg atgaccgccg tgtcgacggg ttcccatccc
     1801 atattttcca tcagccggtg gcgttcaagg atcaccggcc ggaaggcgtc caagatttct
     1861 ccgctgtggg ggcatgtttc gatgacgcga tctatcgttt tttccaatga tttcagactg
     1921 tcatttgctt gcttcacgcg ctccacctct ctcgccgcac ctacggcacg tccttataaa
     1981 tcactaaaaa tcgcgctccg aattttccat cgaagcctct gtctcgcttt ccgatgtcat
     2041 ggagaacgcc aggtgttctc tatcttgttt gaatctagcc gcctgtcatc ctggcgacga
     2101 cttttgaacg ggcctaccag tctggatagg ccgtttcggg gcatctttcc gcctcagtcc
     2161 accaacatga gacagtaaag gttctgcgtc tctgattttt ctttcagtgt ttttatgaaa
     2221 aagccgttgt tccgggcggt ctggaagacg tcgatcccgc aagactccat ggaaggcctg
     2281 gtcttcgccg ggaagcggca gggaatccct tcattcagcc cgcattcctt acagagcacg
     2341 caggggccgg ccaacatcaa gaaggccttg tagtagccgt ccttgaagag ctctccctcc
     2401 agctttacca gggcgtccat ataagcaacc atgttccggg cgcgctcctt tgagtaaggg
     2461 gcatcgacgt ggaacaggat ggcccgccgg taggaatcga ggagagcctg cgtctccgcc
     2521 ggcgtcggcg tatggggcgg gcagctgtaa cggtgcttgt acggacagcc gaagagacac
     2581 ttgaaccgca cccagggggc ggtcactacc gtgctcggat ggatgacctt tgcgtctgta
     2641 gctccagcct gaatcgcctt ttcgcaatac ttatcaagat cggatagctg catcgaaacc
     2701 tccttgtaca gaattgggct cgggtttgtt cacttaacat gtctgctctg cagctgcgat
     2761 gatacggctc gcggaagaaa aatgcaaccc gaagtgaccg ccccgaattg ccccgtgatg
     2821 aaccgatatc cacgtcgaag gcccgttcca catctcattg ccgcccccag gtttcacggt
     2881 gatcaaccgg gtcgtctatt ccaaactcgg cgccttcgaa ctctcggagc aaccgtgagc
     2941 g
//
LOCUS       DAOTMX010000225         2930 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_26489_length_2930_cov_3.749913, whole genome shotgun sequence.
ACCESSION   DAOTMX010000225 DAOTMX010000000
VERSION     DAOTMX010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26489_length_2930_cov_3.749913"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1127
                     /locus_tag="P5573_06735"
     CDS             <1..1127
                     /locus_tag="P5573_06735"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRT27791.1"
                     /translation="EYGADLFLSIHADAARNKSARGSSVYCLSTTGASSVAARLLASK
                     ENLADIIGGAENDQGNDESDPITLHMVQTEAMNLSRNLAASILKQIKAVNPLKFEQVQ
                     YAPFLVLKTPEIPSVLVETAYISNPREEMLLRSPSFQTKIAEAIAAAVDETLTGRKIP
                     PVTIAATVQSEKANPSPDRAKVVEPTIDTVLYKVKKGDHLEKIAKMHGTTVAVLLKLN
                     GMKADGPLYAGSNIKVPAVKKEGEAEKVAQKRPSVQPLEYSFYTVRKGDTLEKIATRN
                     KTTVAVLLRLNNMKLRDPLYVNLRLKVPAASGRENAETVSTKKIREAEPSKPLYYRVK
                     KGDTIERIAKRHDISTAALLKANGMKLKDPLYVGRKLKIP"
     gene            1251..2300
                     /locus_tag="P5573_06740"
     CDS             1251..2300
                     /locus_tag="P5573_06740"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRT27792.1"
                     /translation="MRDSKTKNILFFSLLGFITVLFLYLLKPFFYPIFWAAVIASLSR
                     PFYNWLEGKIRRPSLSASVVLLAVLLIIILPIFLIGALLVNESIAIYASLSEDHSPVK
                     EFLKGVPERVRQNPYVRNLNIDEGLLAEKLSDMAKDVTNFIFTSLKGFTQNTVIFVTM
                     FLIMLYTLFFFIRDGETFLRKAVHLFPLGERREKMLYEQFTSATRATIKGTLVLAFIQ
                     GSLGGLLFFLVGIEGALIWWVLMVFLAVIPGIGCSVVWLPAGIVMLLTGHTWEGITIL
                     LTGTFIISMVDNALRPILVGRDIQMHPLFILFSTLGGIALFGVSGIVIGPIIAALLLS
                     LWQMYDRQYIDKDLL"
     gene            2443..>2930
                     /locus_tag="P5573_06745"
     CDS             2443..>2930
                     /locus_tag="P5573_06745"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRT27793.1"
                     /translation="MSGYIPGEQMPGAKVIKLNTNENPYPPSPTAMAILSNIDTECLR
                     RYPDPFSGRVRRAVSRVFGVHEDWVIVGNGSDEILSILLRACCEPRRAAAYAMPTYVL
                     YRTLAEIQGARCIEVPYDDDYRLPVEGLIAARGALTIVCSPNSPSGTPVSRESLERLS
                     ASV"
BASE COUNT          730 a          814 c          721 g          665 t
ORIGIN      
        1 gggagtacgg tgcggacctc ttcctcagca tccatgccga cgctgcaaga aacaagagcg
       61 ccaggggaag ttcggtctac tgcttgtcca caaccggggc gagtagcgtt gccgcacgac
      121 tgctggccag caaggaaaac ctggccgata tcatcggggg cgcagagaat gaccagggaa
      181 acgatgaatc ggacccgatc actcttcaca tggtccagac agaggcgatg aacctctcga
      241 gaaacctggc ggcgtcgatc ctgaagcaga tcaaggccgt caaccctctt aagttcgagc
      301 aggtccagta tgcacccttt ctggtcctca agacgccgga aataccgtct gttttggtgg
      361 agactgccta catctccaat cccagggaag agatgctgct gcggagtccc tccttccaga
      421 ctaagatcgc agaggctatc gccgccgcag tagacgagac cctgacggga cgcaaaatcc
      481 ctccggtcac aatcgccgcc acagttcaat cggaaaaggc aaacccttct ccggaccggg
      541 caaaggtcgt cgaaccgact attgacacgg tcctctacaa ggtcaaaaaa ggggatcacc
      601 tcgaaaaaat cgcgaagatg cacggcacca ccgttgccgt cctcctgaaa ttaaacggca
      661 tgaaggccga cggcccgcta tatgcgggta gtaatatcaa agtgccggcc gtcaaaaagg
      721 aaggtgaagc cgaaaaggtc gcccaaaaaa ggccttcagt gcaacccctc gagtattctt
      781 tttacaccgt aaggaaaggc gacacactgg aaaagatcgc gacaaggaac aaaacgaccg
      841 ttgccgtcct cctccggttg aacaatatga agctccgtga tcccctgtac gtaaacctaa
      901 gactcaaagt tcccgcagct tccgggcgtg aaaacgcaga aacggtttca acaaagaaga
      961 tccgcgaggc cgagccgtct aaaccgcttt actacagggt aaaaaagggc gacacgatag
     1021 aaagaatagc caaaaggcat gacatctcga ccgcagccct gctcaaagcc aacggcatga
     1081 agctcaaaga cccgctttat gtcggcagaa agctgaaaat cccctgacca aagcggtaag
     1141 accgagagtt ctacctctgg taatttttca aaaacttgta atttaagaga acaatgggta
     1201 tggttctaat attcgatata ttttgacttc tccctatcct ctttgattcc atgagagaca
     1261 gcaaaaccaa aaacattcta ttcttcagcc ttcttggctt catcactgtt ctttttctct
     1321 atcttctcaa gccctttttc tatcccatat tctgggcggc ggtgattgca agcctctccc
     1381 ggcccttcta taattggttg gaaggaaaaa tacgacggcc gagcctttcg gcgtcggttg
     1441 tgctgctcgc cgtccttctg ataatcatcc tgcctatctt cctcatcggg gcgctattgg
     1501 taaatgaatc gatcgcaata tacgcatcat tgagcgaaga ccatagtccc gtcaaagaat
     1561 tcctcaaagg cgttcccgag cgggtcaggc aaaacccata tgtaagaaat ctgaacatcg
     1621 acgaagggct cctggcagaa aaactttccg acatggccaa ggacgtcaca aatttcatct
     1681 ttaccagcct gaaaggcttc acccaaaaca cagtgatctt cgtcaccatg ttcctgatca
     1741 tgctgtacac cctgttcttc ttcatccggg acggcgaaac gtttttgcgt aaggccgttc
     1801 atctctttcc gttgggggaa aggcgtgaaa aaatgctgta tgaacagttc acatcggcaa
     1861 ccagggcaac catcaaggga accctcgttc ttgccttcat ccaaggttct cttggcgggc
     1921 tcctgttttt cctggtgggc atcgaaggcg ccttgatctg gtgggttttg atggtctttc
     1981 tcgccgtcat cccggggatc ggctgctccg ttgtctggct gccggcagga atcgttatgc
     2041 tcctgacggg acacacctgg gaaggcatta caatcctgtt gacgggaaca tttatcatca
     2101 gtatggtcga caatgccctg cggcccatcc tcgtaggaag ggacattcag atgcatcccc
     2161 tgtttatact gttctctacg ctcggaggga tcgccctctt cggcgtttcc ggcatcgtca
     2221 tcggaccgat catcgccgcc ctgctccttt ccctctggca gatgtacgat cggcagtaca
     2281 ttgacaagga ccttctttaa aaaacatagt agaaatatgg gtgatctgtt ttgatcgttt
     2341 tatccggtcc ataaatttca taatttgcta aaatcattat attaataaga attttcgtcg
     2401 tgaggctacc caatgcgtta tttccgaaaa aaaatagaat cgatgtccgg ttacattccg
     2461 ggtgaacaaa tgccgggagc gaaggtgatc aagctgaaca ccaatgagaa cccttacccg
     2521 ccctcaccga cagcgatggc aatacttagc aacatcgaca ccgaatgcct ccgccgctac
     2581 ccagaccctt tttccgggcg ggtacgccgc gcggtgagcc gcgtcttcgg cgttcacgaa
     2641 gactgggtta tcgtcgggaa cggaagcgat gaaatacttt ccatactctt gcgggcctgc
     2701 tgtgaaccgc gccgagcggc tgcgtacgcc atgccgacgt acgtcctcta tcggaccctc
     2761 gcagagattc agggtgcgag gtgtattgaa gtcccttacg acgacgacta ccgactcccc
     2821 gttgaggggc tgatcgctgc gcggggcgcc ttgacgatcg tctgctcgcc gaacagtccg
     2881 tcgggaacgc cggtgtcccg tgaatccctc gagcgcctgt ccgcctctgt
//
LOCUS       DAOTMX010000226         2923 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_26568_length_2923_cov_2.892608, whole genome shotgun sequence.
ACCESSION   DAOTMX010000226 DAOTMX010000000
VERSION     DAOTMX010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26568_length_2923_cov_2.892608"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /gene="amrS"
                     /locus_tag="P5573_06750"
     CDS             <1..652
                     /gene="amrS"
                     /locus_tag="P5573_06750"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HRT27794.1"
                     /translation="RAPVIAFTYNEPTVFTEYLLDIAGEARKLGLRSVLVTCGLMNEA
                     PLADMCRALDAIKVDLKGYSESFYRNVCRAELRPVLRSIKQIAKSRVHLEIVNLVVPT
                     LNDSDEMVRDLITWVLNEVGPDVPLHFTRFHPNYQLLNLPPTPVATLERARDAAMARG
                     LRYVYVGNVPDHPGNNTRCPYCGKEVIKRTSFFVTEVHVKGGRCAFCRRRISGVWG"
     gene            746..2260
                     /gene="amrB"
                     /locus_tag="P5573_06755"
     CDS             746..2260
                     /gene="amrB"
                     /locus_tag="P5573_06755"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HRT27795.1"
                     /translation="MRTSVLIVVMSLAVACLAGNEPVTGRVRDPVVADKFYPSSPVYL
                     RAAVERFMQEAVPVQMKEPVAVIVPHAGYTFSGQVCADGYRQVSGRRYDTVVILGTNH
                     TDRGFEKISVYSGSGFRTPLGVAEVDRAMAAKLVEADSDCVFDESVHEREHSVEVQVP
                     FIQVLFPGAKVVPVVVGSPDVGLCRRFGEALASVVRGTRTLIVASSDLSHYPDVKDVV
                     RVDTETLAAVVELDAGVLHEKVGVLMKSGVENLVTCACGEGSIMAAMEAAKALGAKGG
                     AVVSYHHSGDVPIGESKGVVGYAAVALGEKRALKAREKTPDALAPLSLSPSDKKTLLA
                     FARKSIARFIETRTVPMARGFDPRLSTPRGVFVTLTKRGELRGCIGHVPPDTPLINLV
                     GAMAVKAAFGDPRFEPLGDKELDTVEIEISVLTTPKGVGGVGDVVIGRDGVILQKEGR
                     AALFLPQVAPQQGWGREETLEHLCLKAGLERWCWKENAKIFTFQAVVFGEKDFH"
     gene            2283..>2923
                     /locus_tag="P5573_06760"
     CDS             2283..>2923
                     /locus_tag="P5573_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27796.1"
                     /translation="MRYGMPVILFIIGLLSILGQVVLLRELNVAFYGVELIYLLALGV
                     WLFLSALGALAAHRRLRPSPNGLTVLFLLFGVSVVLSVVFIRASRTVFAAVPGAYLSF
                     PEQLLAMAIALLPVGVLSGLLFQWAARMYIARHKTLIAAYGIESMGGLAGGLLATTCL
                     KWGIQNFSTAVLCGAAAVLVAAVYFSGRRSLLFSVPALGLAGFMLSMLWPPLSP"
BASE COUNT          521 a          858 c          931 g          613 t
ORIGIN      
        1 ccgggcgccg gtaatcgctt tcacgtacaa cgaaccgacc gtctttaccg agtatctact
       61 cgacatcgca ggggaggcaa ggaaactcgg cctgcgctcc gtgctcgtca cttgcggcct
      121 aatgaacgag gccccgcttg ccgatatgtg ccgggccctc gacgccatca aggtagacct
      181 gaagggctac agcgagtctt tctacagaaa cgtctgcagg gcggagctca ggcccgtgct
      241 gagaagcatc aagcagatcg caaaaagtcg agtccacctg gagatcgtga accttgtggt
      301 ccccacgctg aacgattccg acgagatggt gagggacctt atcacgtggg tcctgaacga
      361 ggtcgggccg gacgtaccgc ttcacttcac acgttttcac cccaactacc agcttctcaa
      421 cctccccccc acgcccgtcg cgacccttga acgggcgagg gacgccgcca tggcgcgggg
      481 tttacggtat gtctacgtcg gcaatgtccc cgaccacccc ggcaacaaca cccggtgtcc
      541 ctattgtgga aaagaagtga taaagaggac gtccttcttc gtgaccgagg tgcacgtgaa
      601 gggtggccgc tgcgcctttt gccgcaggag gatctccggt gtgtgggggt agggcagagt
      661 tcgaagttta gaaccggggg ggtcaacaag gccccaaggg cgaaaaggtt caagaagtct
      721 cgagaaggtg atggtggaga aaatcatgag aacgagtgtg ctcatcgtcg tcatgagtct
      781 ggccgttgcc tgtctggccg gtaatgagcc ggtgacgggc cgcgtgcggg acccagtcgt
      841 agcggataag ttctatcctt catcacccgt ttatctcagg gcagcggtcg agcgcttcat
      901 gcaggaggcc gtcccggtcc agatgaagga gccggtggcg gtcatcgtcc cccatgccgg
      961 gtacaccttt tccgggcagg tctgcgcgga cggttaccgg caggtgagcg ggaggcggta
     1021 cgatacggtc gtgattctcg gcaccaacca cacggaccga gggttcgaaa aaatttccgt
     1081 ttattccggc agcggctttc gcacacccct cggcgtcgcc gaggtagaca gggcgatggc
     1141 cgcaaaactt gtcgaggccg acagcgactg tgtgttcgac gaatcggtcc acgagcgcga
     1201 gcattcggtg gaggttcagg tcccattcat ccaggttctc ttcccgggcg cgaaggtcgt
     1261 accggtggtg gtggggtctc ccgacgtcgg cctctgccgg cgcttcggtg aggcgctcgc
     1321 gagcgtcgtc agaggtacca gaaccctgat cgtggcgagt tcggacctct cgcattaccc
     1381 ggatgttaaa gacgtcgtga gggtcgacac tgaaacgctg gccgccgttg tcgagctcga
     1441 tgcgggggta ctccatgaga aggtcggggt gctgatgaaa agcggtgtcg agaacctcgt
     1501 cacctgtgcc tgtggagaag ggtcgatcat ggcagccatg gaagcggcca aggcccttgg
     1561 tgcgaaaggc ggggcggtcg tcagctacca ccattcaggt gatgtcccca tcggggagtc
     1621 caagggcgtc gtcggttacg ctgccgtggc acttggcgag aagagggcct tgaaggctcg
     1681 ggaaaagaca cccgatgcct tggcgccgct ttctctttct ccctccgaca agaaaacgct
     1741 cctcgccttc gcccgaaagt ctatcgcccg gtttatagag acgcgcaccg tccccatggc
     1801 gaggggcttc gacccccggc tttcgacgcc ccggggggtc tttgtcaccc ttaccaagcg
     1861 aggagagctt cggggttgta tcgggcatgt ccccccggac acccctttaa tcaacctcgt
     1921 gggcgccatg gcagtgaagg cggctttcgg tgacccccgt ttcgaaccct tgggagataa
     1981 ggaactcgac accgtggaga tagaaatatc cgtgctcacg acccctaagg gggtcggtgg
     2041 ggtgggcgac gtggtgatcg gcagggacgg cgtcatcctc cagaaggaag ggcgggcggc
     2101 cctcttcctt ccccaggtag cgccgcagca gggctggggg cgcgaagaaa cgctggaaca
     2161 cctctgtctc aaggcggggc tggaaaggtg gtgctggaag gagaatgcga agatctttac
     2221 gttccaggcg gttgtgttcg gcgagaagga cttccactga attttgagtc ttgagctttt
     2281 atatgcgcta cggcatgccc gtcatcctgt tcatcatcgg cctcctctcg atactgggac
     2341 aggtggtcct cctgcgggag ctgaatgtag ccttttacgg cgtcgagttg atctacctgc
     2401 tcgccctcgg cgtctggctt tttctgtcgg ccctgggtgc gcttgcggcg caccggagac
     2461 tcaggccgtc tcctaacggg ctcaccgttc tttttcttct cttcggggtt tccgtagtcc
     2521 tgagtgtcgt cttcatccgc gcgagccgca cggtcttcgc ggccgtaccg ggcgcttatc
     2581 tgtcttttcc cgagcagttg ctggcgatgg ccatcgccct ccttcccgtc ggggtgcttt
     2641 caggcctgct ctttcagtgg gcggcaagga tgtacatagc ccgccataag accctcatcg
     2701 ccgcatacgg catcgagagc atgggaggcc tggcgggagg acttcttgcc acgacctgcc
     2761 tcaagtgggg cattcagaat ttcagcacgg ccgtcctctg cggcgcagcc gccgttctcg
     2821 tcgccgctgt gtatttttcg ggaaggcggt cgctgttatt cagcgtcccc gcgctcggcc
     2881 ttgcggggtt catgctgtcc atgctgtggc cgcctctgtc gcc
//
LOCUS       DAOTMX010000227         2921 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_26595_length_2921_cov_2.622470, whole genome shotgun sequence.
ACCESSION   DAOTMX010000227 DAOTMX010000000
VERSION     DAOTMX010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26595_length_2921_cov_2.622470"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1118
                     /locus_tag="P5573_06765"
     CDS             <1..1118
                     /locus_tag="P5573_06765"
                     /inference="COORDINATES: protein motif:HMM:NF025930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HRT27797.1"
                     /translation="SEKNNRDKIIKYIGSCKEMGIDVLPPDINESMMDFSVVGGKIRF
                     GLAAVKNVGEAAIEAVIRSRQDARFASLGDFLGRVDLKKINKRVIESLIKCGAFDSFG
                     RNRRQLLEGSEKIMEAGNRRRKNGRQSQSSLFALSDEAEGRSEGRDNDIALPDVPEWE
                     TSDLLAFEKETLGFYITGHPLSKYASILAAIPDVVDTESIVDRKDGETVKIAGVVSSI
                     RTVLTKKKDTMAYVTLEDMKGSVTVIVFSDVFRKTSPFLQSGEPVLVTGSIDADDESM
                     KVIASDISLLSDWRESPHSSVHFDINASTIDANSLETLKNIISQHKGKLDGYIHLYLN
                     GQSETVIYLGKDMRLNAADELKTAADAVLGRGSTRFT"
     gene            1118..1864
                     /locus_tag="P5573_06770"
     CDS             1118..1864
                     /locus_tag="P5573_06770"
                     /inference="COORDINATES: protein motif:HMM:NF003323.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HRT27798.1"
                     /translation="MNPRDKSPSSYKERTYRNRLSSPGKGVSFNVAVKETDLWVCAER
                     DLSRETRESVLRCRHSIEEYIRVHPEFLSSFSPLRLVPLAPPVVQDMLEAAAAAKVGP
                     MASVAGAIAQHVSEDLLRYTKEVIVENGGDIYMMCGREVRVGVFAGKSPLSFKVALKV
                     GPADMPVGVCTSSATVGPSVSLGRADAVCVLSSSAALADAAATAIGNMVKRDTDIQGA
                     LERGMEIPGVRGALVIVGKKIGLIGAIELY"
     gene            2153..>2921
                     /locus_tag="P5573_06775"
     CDS             2153..>2921
                     /locus_tag="P5573_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27799.1"
                     /translation="MAVAQDWTTNKDLIQELYVAFYGRPGDPGGIKYWAEQLPDNATL
                     ADTTTKDLVRSFIRSGEARTRIGNVDDPLMHDSIIEKIYFQLFHREVLQNEIDSFKDK
                     PIEDVLVSILNPAAGTTDATTLSTKLQYANWFVQALDPNGDGIANDSADGTHFAAEFN
                     GNVDASDVAAKMAFIDYKTTTLTKADVLTDIQTLVVDPGEVNEANDIPLAAPTITTPV
                     AGDNMVDAAERASGFTVTGRAEAGARVTLTVTDGAGHA"
BASE COUNT          703 a          821 c          834 g          563 t
ORIGIN      
        1 cgagcgagaa gaacaaccgc gacaagatta tcaagtacat cggaagctgc aaggaaatgg
       61 gtatcgacgt ccttcccccc gatatcaacg aatccatgat ggatttcagc gtcgtcggcg
      121 ggaagataag gttcggcctc gcggccgtca agaacgtcgg cgaagccgcg atcgaggctg
      181 tcatccggtc gcggcaagac gcaaggtttg cgagcctcgg cgacttcttg gggagggtcg
      241 acttaaagaa gataaacaag cgggtcatcg agagcctgat aaagtgcggc gccttcgact
      301 ccttcgggcg aaaccggagg caactcttgg aaggctccga aaagatcatg gaggcgggca
      361 accggcgccg caagaacggg cggcagtcgc aatcgtccct cttcgccctt tccgacgagg
      421 ccgagggccg ttccgaaggc cgcgacaacg atatcgcgtt gcccgatgtc cctgaatggg
      481 agacctccga cctcctggcg ttcgaaaaag agacactcgg cttctacatc accggccatc
      541 ccctttctaa gtacgcgagc atcctcgccg ccatcccaga cgtcgtcgac acggaaagca
      601 tcgtcgacag gaaagacggc gagaccgtta agatcgccgg cgttgtgagc agcatcagga
      661 ccgtgctcac caagaagaaa gacacgatgg cctacgtcac cctcgaagac atgaagggct
      721 cggtgaccgt catcgtcttc agcgacgtct tcagaaagac gtcccccttt ctccagagcg
      781 gcgagccggt cctcgtcacc gggagcatcg acgccgacga tgagagcatg aaggtaattg
      841 cctcggatat ttccctcctc tccgattggc gtgaatctcc gcatagttcc gtccatttcg
      901 acataaacgc ttcaactatc gatgccaact cactggaaac attgaagaat atcataagcc
      961 agcataaggg aaaattggac ggctacatcc atctctacct gaacgggcag tcggagacgg
     1021 tcatctacct ggggaaagac atgcgattga atgccgccga tgagctgaaa acggcggcag
     1081 atgccgtact tggtcgcggt tctacacgtt ttacttgatg aatccgcggg acaaatcccc
     1141 ctccagttat aaagaaagga cctaccggaa cagactgtcg tcccccggga agggggtctc
     1201 tttcaacgtc gcggtgaaag agacagacct ctgggtatgc gcagagcggg acctctcccg
     1261 cgagaccagg gagtcggtct tgcgctgccg ccattccatc gaagagtaca tccgggtcca
     1321 cccagaattc ctctcgtctt tcagcccgtt aaggctcgtc ccgttagcgc ctccggtagt
     1381 tcaggacatg ttggaggccg cggcggcggc gaaggtcggg ccgatggcct ccgtggccgg
     1441 tgcgatcgcc cagcatgtct cagaggacct actcaggtac actaaggaag tcatcgtcga
     1501 aaacggtggt gacatataca tgatgtgcgg ccgggaggtg agggtaggtg tcttcgccgg
     1561 caaatccccc ttgagtttca aggtcgccct gaaggtcggg cccgccgata tgcccgtcgg
     1621 cgtctgtacc tcctcggcaa cggtaggccc atcggtgagc ctcggcaggg ccgacgccgt
     1681 ctgtgtcctc tcctcatctg ccgcccttgc cgacgccgcg gcgaccgcga taggaaacat
     1741 ggtaaaaaga gatacggata tccagggcgc cctcgagagg ggcatggaga tacccggtgt
     1801 acgcggcgcc ttagtcatcg tgggcaagaa aatcgggctg atcggtgcga tagagttata
     1861 ttgagcattc tgcggaatcc agctcctccc gtcataccgg cacgtcgtta ctgggacccc
     1921 ccgattttta tcggggtgac gcgcctgggt gacgaccatt gtacaaggac gtccttattc
     1981 cttttctttt ctcttgacat catttgaaca tattattaca aatcacttta gattacggcg
     2041 agatagataa cttaggggcg tatgaaaata cgccctgtgt tttgaagaca cagcgctacc
     2101 tgtcacgacg ggggcgtaaa aattctttgc tgattcaaag gaggaaaagg gcatggcagt
     2161 ggcacaagat tggaccacca acaaagacct gattcaggag ctgtacgtcg ccttttacgg
     2221 taggccgggg gacccgggag gaatcaagta ctgggcagag caattaccgg acaacgcgac
     2281 cttagcagac actactacaa aggacttagt taggtcgttc attcgttcag gtgaggcgag
     2341 gacgcgcata ggtaacgtag acgacccgct gatgcacgac agcataatcg agaagatata
     2401 cttccagctc tttcatcgag aagtattgca gaatgagatc gactccttca aggacaaacc
     2461 catagaggac gtattagtca gtatactcaa ccctgccgca ggaacgacgg acgcaaccac
     2521 cctcagcact aagctgcagt atgcaaactg gtttgtgcag gccctcgacc cgaacggcga
     2581 cggcattgcc aacgactctg ccgacggcac acacttcgcg gcggaattca acggcaatgt
     2641 ggacgcatcg gacgtggccg ctaagatggc cttcatcgac tacaaaacaa ccaccttgac
     2701 aaaggctgat gtgcttacag acatccagac cctcgtagtt gacccgggag aagtgaacga
     2761 agcaaatgat ataccccttg cggccccgac gatcacaact ccggtagccg gcgacaacat
     2821 ggtggacgct gcagaaaggg cctccggttt caccgtcacc ggcagggcgg aggcgggcgc
     2881 cagggtgacg ctgaccgtga cggatggcgc gggtcacgcg g
//
LOCUS       DAOTMX010000228         2916 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_26650_length_2916_cov_4.376442, whole genome shotgun sequence.
ACCESSION   DAOTMX010000228 DAOTMX010000000
VERSION     DAOTMX010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26650_length_2916_cov_4.376442"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..1220)
                     /locus_tag="P5573_06780"
     CDS             complement(51..1220)
                     /locus_tag="P5573_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HRT27800.1"
                     /translation="MGKSKVQKSYREINEKIKKGKAVVVTAEEMIDIVRDRGDVEAAR
                     YVDVVTTGTFSPMCSSGAFLNFGHTSPKIKAMKVWLNDVQAYGGLAAVDCYIGATEVA
                     EGDPLNSVYPGEFNYGGGHVIEDLVAGNAVKLRVEGYGTDCYPARKFEKNITIGDLRD
                     AILFNPRNAYQNYNCAVNMSDKTIYTYMGILRPRMGNANYSTSGQLSPLFNDPYYRTI
                     GIGTRIFLGGAQGYVAWHGTQHNPRVERGRNGVPKAGAGTIATIGDLKVMSPEWLVGA
                     SILGYGVSLYVGIGIPIPILDEEMARYTAVSDEDIFTQVYDYGMDYPKGELKSLGEVS
                     YKELRSGSITIKGKVIPTAPLSSYHKARQIAGILKDWISKGAFFLGEPQELLPTD"
     gene            complement(1283..1729)
                     /gene="dut"
                     /locus_tag="P5573_06785"
     CDS             complement(1283..1729)
                     /gene="dut"
                     /locus_tag="P5573_06785"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001862.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HRT27801.1"
                     /translation="MEKIEIPVTRLPDNEDIPLPCYITGQAAGMDIFAAVRDEETIFS
                     GERKVIPSGIAIALPEGFEAEIRPRSGLAVRYGVTVINSPGTIDADYRGEILILLINH
                     GQEPFVVKRGDRIAQMVVHRVCRVVWHPCDELGCTQRGAGGLGHTG"
     gene            complement(1782..>2916)
                     /locus_tag="P5573_06790"
     CDS             complement(1782..>2916)
                     /locus_tag="P5573_06790"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRT27802.1"
                     /translation="NGAAHFIEHMLFKGTKRRTAFDIASEIDSVGGILNACTGKELTS
                     FYVKIPDYHLPLAIDLLADLLKDSVFDPEEMEKEKSVILQEIRMVEDTPDDFIHDFFG
                     KAFWDGHPLSLPVLGTEDSVRGIGRETLLDIFNRYYRGSRLVLTVAGRLRHNELVSLV
                     GEALGSVTPAEGGNGQGAPTPVAKVAVLEKDLEQVHMIIGSPGHTFSDPARYAGFIMN
                     TVLGGSMSSRLFQEVREKRGLAYAVHSYLVSYRDAGMFGIYVGTADEKVGEVLDVIFD
                     ELRKLKADLLTDKELFSAKEQIKGRFLLSMESTDTRMTRLAKNEIYLGRHLSFDEAIA
                     NLEAVSAEEVRGVARTLLNKDAVSVAAIGRVTERDITANMPDL"
BASE COUNT          620 a          891 c          734 g          671 t
ORIGIN      
        1 gcaacaacca aaacttttga caccctcgaa ttcgtgagat cggttgtttt ttaatcggtc
       61 ggcagcagct cctgcggttc gcctaggaaa aaggccccct tcgaaatcca atccttcaaa
      121 atccccgcaa tctgccgtgc cttgtgataa ctggagagcg gagcggtggg gatcactttg
      181 ccctttatag tgatcgaccc gctcctcaat tctttgtaac tgacttcacc gaggcttttc
      241 aattcacctt tgggataatc catgccgtaa tcatagactt gggtgaagat gtcttcgtcg
      301 ctgacggccg tgtaccgcgc catttcctcg tcgaggatcg ggatggggat gccgatcccc
      361 acatagagag aaacgccgta tcccagaata ctagcaccga ccagccattc cggcgacatc
      421 actttgaggt cgcctatcgt cgctatagtt cctgcgcccg ccttggggac accgtttctg
      481 ccccgctcca cgcgggggtt atgctgagtc ccatgccagg ctacatatcc ttgggcgcct
      541 cccagaaata tcctcgtccc tatcccgata gtcctgtaat aagggtcatt gaagagcggg
      601 ctcagttgcc ccgacgtcga gtagttggcg ttgcccattc gcggccgaag aatgcccatg
      661 taagtgtaga tcgtcttgtc ggacatgttg actgcacaat tgtaattctg ataggcattc
      721 ctggggttga acaggatggc gtcacggagg tctccgatgg taatgttttt ctcgaattta
      781 cgggctgggt agcagtcggt gccgtaccct tcgaccctca atttaaccgc gttaccggcg
      841 acaaggtcct cgatcacatg cccgccgccg tagttgaact cgccgggata aacgctgttg
      901 agagggtccc cctcggccac ctcggtcgcc ccgatataac agtccacggc cgccagacca
      961 ccgtaagcct gcacatcatt cagccacacc ttcatggcct tgatcttggg ggacgtatgg
     1021 ccaaaattga ggaaggcccc cgaggaacac atcggcgaaa aagttcctgt cgtcaccaca
     1081 tcgacgtacc gcgccgcctc cacgtcgcca cggtctcgca cgatgtcgat catttcctcg
     1141 gccgttacga ctaccgcctt acccttcttg attttttcgt tgatctcccg gtaggacttt
     1201 tgaactttag acttacccat gaacgcctct tcctccggca tagctcaacc cttcactatg
     1261 cccacctact cccgaccgcc tttcaccccg tgtgtcctaa accccccgcc ccccgctgcg
     1321 tgcagcccaa ctcatcgcac gggtgccaga cgacacggca gacgcgatgc accaccatct
     1381 gggcgattcg gtcgcctctt ttgacgacaa agggctcctg cccatgattg atcaacagta
     1441 tcaagatttc gccgcggtag tccgcatcga tggtcccggg ggaattgatg acggtcacgc
     1501 cgtaccgaac ggcaagcccg ctccggggcc tgatctcggc ctcaaaacct tcgggcaggg
     1561 cgatggcgat tcccgagggg atcacctttc tttcaccgga aaatatcgtc tcttcgtccc
     1621 tgaccgccgc aaagatgtcc atccccgctg cctgtcccgt tatgtaacag ggaagaggaa
     1681 tatcttcatt atccggcaac cgcgtaacgg gaatctctat tttttccatc atacctcgtg
     1741 gaggaaagga cccagcagcc ggagagccaa gcgattcgag atcaaaggtc cggcatatta
     1801 gcggtgatgt ccctctcggt aaccctgccg atcgcggcga ccgaaactgc gtccttattg
     1861 agcaaggtgc gcgccactcc ccgtacctcc tccgcactca cggcctcgag gttggctatc
     1921 gcctcgtcga acgacagatg ccggccgaga tatatctcgt tcttcgcaag ccgcgtcatc
     1981 ctggtgtccg tactttccat gctcaacaga aaccgcccct ttatctgctc cttggcgcta
     2041 aagagttcct tgtcggtcag aagatcggct tttagcttgc gcagctcatc aaagattacg
     2101 tcgagaactt cgccgacttt ttcatcggcg gtcccaacat agattccgaa catgcctgca
     2161 tccctgtatg acacaagata agaatggaca gcataggcaa gccctctttt ctcgcggacc
     2221 tcctggaaca gcctcgagct catgctgccg ccgaggacgg tgttcattat aaaaccggca
     2281 tacctggccg ggtccgagaa ggtatgcccc ggggagccga tgatcatgtg aacctgttcc
     2341 aagtcttttt cgagcacggc gactttcgcc accggagtcg gggctccttg cccgtttcct
     2401 ccttccgccg gcgtaaccga gccgagggcc tcgccgacca aactcacaag ctcgttgtgc
     2461 ctgaggcgcc cggccacagt caaaacgagc cgacttcccc ggtaatatct attgaagatg
     2521 tcgagaaggg tctccctccc gatgcctctg acggaatctt ccgtaccgag aacgggaagg
     2581 gaaaggggat ggccgtccca gaacgccttc ccgaaaaaat catggatgaa atcgtccgga
     2641 gtgtcttcca ccatacggat ctcctgaagg ataacggact tttctttctc catttcctca
     2701 gggtcaaaga cggagtcttt caagaggtcg gccagaaggt cgatggcaag gggaagatgg
     2761 taatcgggaa tcttcacgta aaaggaagtc agttctttac cggtacatgc attgagaatg
     2821 ccccccacgg aatcgatctc ggaggcgatg tcgaaggcag tcctcctctt tgttcccttg
     2881 aagagcatat gctcgatgaa atgggctgcc ccgttg
//
LOCUS       DAOTMX010000229         2907 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_26749_length_2907_cov_2.908836, whole genome shotgun sequence.
ACCESSION   DAOTMX010000229 DAOTMX010000000
VERSION     DAOTMX010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26749_length_2907_cov_2.908836"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /gene="nfi"
                     /locus_tag="P5573_06795"
     CDS             complement(<1..766)
                     /gene="nfi"
                     /locus_tag="P5573_06795"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: protein motif:HMM:NF008629.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HRT27803.1"
                     /translation="MKIRKIHDWDVADYHGAVRIQESLRPRLILGGEIEEVGTVAGAD
                     ISYARGDDFFYAAVVLLRLPDFATIEESFASGRVSFPYVPGLLSFREGPILLKAFEGL
                     QNVPDLVLIDGQGIAHPRGLGLAAHMGLFLDVPTVGCAKTRLVGTHGQAGPNVGDWTG
                     LFLGGEVVGAVLRTKRNVNPIFVSPGHRVSLEGAVSAVVQCLAGYRIPEPVRRAHLAV
                     APAAPGVTSAAMASGILFALAAGLMWGLVFIGPLLLP"
     gene            1130..2278
                     /locus_tag="P5573_06800"
     CDS             1130..2278
                     /locus_tag="P5573_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27804.1"
                     /translation="MKSFLRGVVFLLSFLGIFNLTTETALAQAKTVITEGEAAIVNND
                     IPSARQEAVARAKWAAIEQVVGVQVKAESFVQNMVLVEDVMKSQVTGVVKKFDVLDQQ
                     KKEGDIYWVRVRCTVEPAKAQQAVSTLALNGSIALFIPARKPQAGGGSQFEETNILSE
                     TLIGKLTAQDYKVIDVAPTGAADAAEIENAVKGGHSLLLRSLMYKFLSNLLIIGKTDY
                     TISTKKGEDIGYGLSMPFNNVTVRLTYRVVAKNNKTGTVEVLTAGAEQAKGLANNVED
                     AAASALKDLAEKIAPKILDTVAKYIEGNAKKVQVKVTGVSDLDTNMEVKGILQNIVWV
                     TGVEDKEMGEYIVTYPESTIYLANSIQQKGNFKIINFTPYSIDLEYQK"
     gene            2333..>2907
                     /locus_tag="P5573_06805"
     CDS             2333..>2907
                     /locus_tag="P5573_06805"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HRT27805.1"
                     /translation="MACVDMPVKYDPLSFAPDPPDKENIPRICKSTYESFFPRVAVVN
                     FTNNTTFDYAEVVQTHVQGQGQRQAVGGAAVGVAPGAAGVVWGARERQQFQRDAQRIQ
                     RQINAKLSESVEDGVMNELVSMGGAKVFTRTEMHKILNEHKFQQSGLVDDTQLVRLGK
                     LAGIKYMVTGSVNNVNLVWRDYGSQQMGQQA"
BASE COUNT          736 a          844 c          807 g          520 t
ORIGIN      
        1 caggcagcag cagcggcccg atgaagacca ggccccacat caggccggca gccagggcga
       61 acaggattcc agaagccatg gcggccgagg ttaccccggg tgcggcaggc gccacggcaa
      121 ggtgcgcccg gcggacgggc tcggggatcc tgtagcctgc aaggcactga acgaccgcgc
      181 taacggcccc ctcgaggctc accctatgac ccggggagac aaatatcggg ttcacgttcc
      241 tcttcgtcct gagtacagcc cctactactt ccccgccgag gaacaaaccg gtccaatccc
      301 ccacgttcgg gcccgcttgg ccgtgggtcc cgacgagccg cgtcttggcg catccgacgg
      361 tggggacgtc gagaaagagg cccatgtggg ccgcgaggcc gagaccccgg gggtgggcta
      421 tcccctggcc gtcgatgaga accaggtcgg ggacattttg caacccctcg aaggccttga
      481 ggaggatggg gccttcccgg aaactcaaga gccccgggac gtagggaaaa ctcacccgac
      541 cggaggcgaa agattcttca atggtcgcaa aatcaggaag ccgcaacagg acgacggcgg
      601 cgtaaaaaaa atcgtcgccc cttgcatagg agatgtccgc acctgctacc gtcccgacct
      661 cctcgatctc gccgcccagt atgagacggg gtctcaaact ttcctggatt cgaacggctc
      721 catggtagtc cgccacatcc cagtcatgga ttttccttat cttcacccgg gaaccatcct
      781 catctgccca aaattgcccc cctcaggccc tctactaatc caccagaaac cgcctctcag
      841 ccaaagagag acctttgaaa aacggcccct catgtcattc cgacgaaagt cggaatccag
      901 aaccgcttga aaacactgga ttcccctgta tcgagtacgg ggcaggctta agtcaagcac
      961 ggaatgatga aaaggcgatt taattacaaa ttcaaaggtc tcaaaaatag aacacagttt
     1021 ggcattaatc cattatttga ccgttttttc aagaaattat gacaaaaaat ctgcttgtaa
     1081 actcccctgc aatcggttat caaacagcca aaagcacaag gggttgtcta tgaagtcgtt
     1141 ccttcgagga gttgtatttc ttttgtcttt cctgggcatc tttaacctca cgacagagac
     1201 ggccttggcc caggccaaga ccgtgatcac ggagggagaa gcggcgatcg tgaacaacga
     1261 tatcccctcc gcaagacagg aggcggtcgc acgggcgaag tgggcagcca tcgagcaggt
     1321 cgtcggcgtc caggtaaagg ccgagagttt cgtccagaac atggtccttg tcgaagacgt
     1381 catgaagtcc caggtcaccg gcgtcgtcaa gaagttcgac gttctggacc agcagaagaa
     1441 agaaggcgat atctactggg tgagggtccg gtgcacagtc gaaccggcca aagcccaaca
     1501 ggccgtctca acccttgccc taaacggctc gatcgccctc ttcatcccgg cccggaaacc
     1561 acaggccggt ggtgggagcc agttcgaaga gaccaacatc ctctccgaga cactgatcgg
     1621 caaactgacg gcacaggatt acaaggtaat cgacgttgcc ccgacgggtg cggccgatgc
     1681 cgcggagatt gaaaacgccg tgaagggcgg ccactccctc ctgctccgct ccctgatgta
     1741 caaattcctc tccaacctac tgatcatcgg caagactgat tatacgattt ccacgaagaa
     1801 aggcgaagac atcggctacg gcctaagcat gccgttcaac aatgtcaccg tgagactgac
     1861 ttaccgggtc gtggcgaaga acaacaagac gggaaccgtc gaagtactca cggcaggtgc
     1921 cgaacaggca aaggggctcg ccaacaacgt ggaagacgcc gccgcatcgg ccctcaagga
     1981 cctggcggaa aaaatcgccc cgaagatcct cgacacggtc gccaagtaca tcgaggggaa
     2041 tgcgaagaag gtccaggtga aagtcacggg tgtctccgac ctcgacacga acatggaggt
     2101 caagggtatc ctccagaaca tcgtctgggt gaccggtgtg gaggataaag agatgggcga
     2161 gtacatcgtg acttaccctg agagcaccat ttatcttgca aacagcatcc aacaaaaagg
     2221 caatttcaag attatcaact tcaccccgta ttccatcgat ttggaatacc agaaatgagg
     2281 aggagttatg cgtggcaggt ttgcactgtt tttagtaata tctattgtat taatggcttg
     2341 cgtggacatg cccgtcaagt atgaccccct gtccttcgcc cccgaccctc cggacaagga
     2401 aaatatcccc cggatatgta agagtactta cgaaagcttc ttcccgcggg tagccgtcgt
     2461 caacttcacg aataacacga cctttgacta cgcagaagtg gtacagaccc atgtccaggg
     2521 tcagggtcag cgacaggccg tcggcggggc cgctgtcggc gtcgcgcccg gcgcggccgg
     2581 tgtcgtctgg ggagcaaggg aaaggcaaca attccaacgg gacgcccagc gaatccagcg
     2641 ccagatcaac gccaaactga gcgagagcgt cgaagacggc gtgatgaacg agctcgtgag
     2701 catgggcggc gcgaaggtct tcacccgcac cgagatgcac aagatcctca acgaacacaa
     2761 gttccagcaa tcaggactcg tggatgacac ccaactcgta aggctgggaa aactggccgg
     2821 gatcaagtac atggtgacgg gttcggtcaa caacgtcaac ctcgtctgga gggattacgg
     2881 ctcccagcag atgggacagc aggcaca
//
LOCUS       DAOTMX010000230         2881 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_27033_length_2881_cov_3.999292, whole genome shotgun sequence.
ACCESSION   DAOTMX010000230 DAOTMX010000000
VERSION     DAOTMX010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27033_length_2881_cov_3.999292"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..587
                     /locus_tag="P5573_06810"
     CDS             102..587
                     /locus_tag="P5573_06810"
                     /inference="COORDINATES: protein motif:HMM:NF025070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HRT27806.1"
                     /translation="MKADTATFRKLLTEAIFETFEKMFFLFLEPIDRDPEGCDLESAV
                     QFKGFIEGRLEVSLSRNLATAMVSNMLSCSTGDVTDAKIEDCAKEAANVICGSFLQRL
                     DRRGDFNLSTPSFRRVAGVNRNVSEGEGMIRICFDSDCGGLSVRAFVSEHRPHPGRGP
                     E"
     gene            621..1667
                     /locus_tag="P5573_06815"
     CDS             621..1667
                     /locus_tag="P5573_06815"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HRT27807.1"
                     /translation="MLDQNCLDKKIRVLVVDDSAIVRKIFSEELSRYPDIEVVGTAPD
                     PFVARDKIMRLKPQVITLDIEMPRMDGLTFLRKLMRYQPLPVIIVSSLTKEGGKLALE
                     AIESGAVDVVSKPGSSFTVGDMREQLVAKIRAAALARLPRPETKPVSTPEPMTALTCT
                     THKVIAIGASTGGTEALKCILTKMPPNSPGTVVVQHMPANFTTAFANRLDGLCQTRVK
                     EAADGDAVIPGTVLIAPGNYHMLLKRSGARYYVEVKDGPRVHHQRPAVDVLFKSAAKF
                     AGANAVGVILTGMGADGAEGLLEMKRAGAATIAQDEKTCVVFGMPKEAIRLGGVDKVA
                     PLEQISQEILKMVG"
     gene            1670..>2881
                     /locus_tag="P5573_06820"
     CDS             1670..>2881
                     /locus_tag="P5573_06820"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HRT27808.1"
                     /translation="MDVDSLIHAIDAMASDFLLLERGTVDVPTAGRLLNQLEIISGGA
                     LELDAEPMRRLSGGLVALLENVIMNGGGGEADFTALQKGITLMQEVGSGLTKTDAPRG
                     KDVGEFMSEITQLAGDGFPEETPSAAKEQEGGGASGLQDESLLRDFIVEGLEYINEIE
                     VNILNLEQNPEDKDCVNSLFRPFHSIKGVAGFLNLDDIRELAHRLEDLLDKIRKDELS
                     VNTDVIDVILEGADALKALIVSLQKKLEGHPSADPEVDLEALAARIGDVEEGNHAEKG
                     VKRLGEILVEDGVITRETLTECLDVAKETPAKKIGEALIAGGKATPKQVSQALRKQIS
                     QASEAASIRVDTSKLDDLIDMVGELVITQTMIRQNPVLRETADEKLLRDISQLGSIIS
                     ELQRTSTSLRMI"
BASE COUNT          682 a          704 c          920 g          575 t
ORIGIN      
        1 aatccgctcg gtcatgagca agggagcgaa aaggttcgtc aggaaacctt tcctgccgga
       61 agacatcagg aaaattttgt atgaggtcat aggtgtggat aatgaaggca gatactgcga
      121 cgttcaggaa actcctgacg gaggcgattt ttgaaacctt cgagaagatg ttctttcttt
      181 ttcttgaacc gatagaccgg gatccggagg gatgcgacct ggaatcggcc gttcaattca
      241 agggcttcat cgaaggacgc ctggaggtgt cgctttcgag aaacctcgca accgctatgg
      301 tgagcaatat gctcagttgc agtacggggg acgtgacgga cgctaagatc gaagattgtg
      361 cgaaggaagc ggcgaacgta atctgcggta gctttctaca gaggttggac aggcgcggtg
      421 atttcaacct gtcgacccct tctttccgcc gggtagccgg agttaatcgg aatgtgtcgg
      481 aaggggaagg aatgatcagg atatgttttg actcggactg cggggggctg agcgtgcgtg
      541 cgttcgtgtc ggaacatcgc ccgcaccccg gtcgcggacc ggaataagct tgaagcaata
      601 cggttgtctg ctgatttact atgctggatc agaactgcct cgacaaaaaa ataagagtcc
      661 tcgtcgttga cgactctgca atcgtgagga agatcttttc cgaagagctg tcccgctatc
      721 ccgacatcga ggtcgtgggg accgcgccgg atccgttcgt cgccagggac aagatcatga
      781 gactgaagcc ccaggtcatc acactcgaca tcgagatgcc gcggatggac gggctgacct
      841 ttttgaggaa gctcatgcgc taccagcccc ttcccgtcat catcgttagt tctttgacga
      901 aagaaggggg caagctggcg ctcgaggcga tcgagagtgg tgctgtggat gtagtttcca
      961 agccgggaag ttcttttacg gtaggagaca tgagggaaca gttggttgcc aagattcggg
     1021 cggcggcttt agcccgctta ccgcgtccgg aaacgaagcc cgtcagtacg cccgaaccga
     1081 tgacggccct aacttgcacg acacataagg taatcgccat cggggcatcg acggggggta
     1141 cggaggccct caagtgcata ctcaccaaaa tgcccccgaa ctcgccgggg accgtcgtgg
     1201 tgcagcatat gccggcgaac ttcacgacgg ccttcgccaa taggcttgat ggtttgtgcc
     1261 agacccgggt aaaagaagcc gccgacggcg atgcggtgat accgggaacg gtgctcatcg
     1321 ccccgggaaa ctatcatatg ctgctcaaga ggagcggtgc ccggtattac gtcgaggtaa
     1381 aggacggtcc cagggttcat caccagcggc ctgcggtcga cgtgcttttc aagtcggcgg
     1441 cgaaattcgc cggggcgaac gccgtcgggg ttattctcac cggtatgggg gcggacggcg
     1501 ccgaggggct gctcgagatg aaacgcgctg gagctgcgac gatagctcag gacgagaaga
     1561 catgcgtcgt gttcggaatg cccaaagagg ccatccggtt gggcggtgtc gacaaggttg
     1621 ctcctctgga gcagatcagc caagagatac tgaagatggt gggatgatta tggacgtcga
     1681 ttctttgatc cacgccattg atgcaatggc ttctgatttt cttctgctgg agcgtgggac
     1741 ggtcgatgtc ccgacagcgg ggcgattgct caatcaactg gaaatcattt ccgggggggc
     1801 gttggaactg gatgccgaac ccatgagacg actttcggga ggtttggttg cgctcttgga
     1861 aaacgtcatc atgaacggcg ggggtggcga agctgatttt accgctctgc aaaaggggat
     1921 taccctgatg caggaggtcg gttcgggatt gacaaagacg gatgcgccgc gtgggaaaga
     1981 cgtgggagag ttcatgtcgg aaatcacgca gctggccggt gacggttttc ccgaagagac
     2041 tccgtccgcg gccaaggagc aggaaggcgg tggggcgtcg gggctccagg atgaatcgct
     2101 actccgggat ttcatcgtag aaggtctcga gtatatcaac gagatcgaag tgaacatcct
     2161 gaacctcgaa cagaacccag aagacaagga ttgcgtgaac tccctcttca ggccctttca
     2221 ctcgatcaaa ggggttgccg gttttctcaa cctcgacgac atacgggaac tggctcacag
     2281 actggaagac cttctggaca agatcaggaa agatgagctt tccgtcaata cggacgtgat
     2341 agacgtgatt ctcgaggggg cggacgccct caaggcgctg atcgtgtctc tgcagaaaaa
     2401 actggaggga cacccgtcgg cagaccccga ggtcgacttg gaggctctcg ctgccaggat
     2461 cggagatgtg gaagaaggaa atcacgcgga gaagggagta aagaggctcg gtgaaatcct
     2521 ggtcgaagac ggggtgatca cgagggagac attgaccgaa tgtctcgatg tcgcaaagga
     2581 gacaccggcg aaaaagatcg gcgaggcgct gatcgccgga gggaaagcga cgccgaaaca
     2641 ggtttctcag gccctcagga aacagatcag ccaggcgagc gaggccgcgt ccatccgtgt
     2701 ggatacctcg aaactcgacg acctgatcga tatggtgggc gaactcgtga tcacgcagac
     2761 catgatcagg cagaatcccg ttctccggga gaccgctgat gaaaagctgc tgagagacat
     2821 atcccagttg ggaagtatca tctcggagct ccagaggacg tcgacaagcc tgaggatgat
     2881 a
//
LOCUS       DAOTMX010000231         2876 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_27094_length_2876_cov_3.357675, whole genome shotgun sequence.
ACCESSION   DAOTMX010000231 DAOTMX010000000
VERSION     DAOTMX010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27094_length_2876_cov_3.357675"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1221
                     /locus_tag="P5573_06825"
     CDS             <1..1221
                     /locus_tag="P5573_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HRT27809.1"
                     /translation="LQFNVLSFDRKRSNVVLSRRSIMEKERNEEKKRVLETIEEGKIV
                     EGTVKNITDYGIFVDLGGIDGLLHVTDISWGKITRPSDSFSKGDKITVKVLSFDRAKE
                     RVSLGLKQLKENPWDTIDEKYAVGSVVEGKVVSIMEYGAFVELEPGVEGLIHVSEMFW
                     TSRKSKHPSKAVSVGEDVRVMILDVNKEKKRISLGLKQTTPNPWNAIKEKYPPGSIVK
                     GVVRNITDFGVFVGLEEGIDGLVHVSDISWRRKVKHPSEFFKKGQEVEAVVLGVDVEN
                     EKFSLGIKQLERNPWDEITAKYPVGSSVSGPITNVTDFGVFVAIEEGIEGLVHISELS
                     QKKVKSLAENFKVGDVVTASVKNVDTKSKKIRLSIREYENSLNETSGNQYMNNREKVG
                     SNLGKVLAGVKLDV"
     gene            1239..2138
                     /gene="sppA"
                     /locus_tag="P5573_06830"
     CDS             1239..2138
                     /gene="sppA"
                     /locus_tag="P5573_06830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HRT27810.1"
                     /translation="MRRHPFLVGILILLTVGFLVFLIFFGATSAVGRGFSPFRDRVGV
                     VPIKGVIMESKSAVDLINRYGEDSRVKAIVVRIDSPGGGVAPSQEIFDALSAVKKKKK
                     VVASMGSVAASGGYYIACAADRIVANPGTLTGSIGALMHFANVEELLKKVGLKSAVIK
                     SGKYKDIGALTREMTPEERALLQGVVDDIYDQFLGTVSASRNISVEELRPVADGRIFT
                     GRQALKMRLVDELGDMERSVDVAANLAGIKGKPEVVYPKEKGVSIWRFIADEMAFSIV
                     NRLRDEAEKVGYQGASWIPLVNS"
     gene            2175..>2876
                     /locus_tag="P5573_06835"
     CDS             2175..>2876
                     /locus_tag="P5573_06835"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRT27811.1"
                     /translation="MLKEEETQYRTIKVEPQEDFLVVCLNRPKEMNALSKEMRWELES
                     CFSSLDEDDSVKAVVVTGGDYVFSAGMDIKEMEALPDGEIDDYFKSIIRYLKKIMNSR
                     KPVIAAVGGIALGGGFNLATVCDIIIASESAIFGHPELKFGLNPLFDPLWRIVGMAKA
                     KEIAMLGEPIGAMEALRIGLVNKVAPPDRFMDEAFTMARELSKRPVKALEAVKRISDI
                     VPRLDRGAALEHEFEI"
BASE COUNT          748 a          612 c          893 g          623 t
ORIGIN      
        1 ctccagttta acgttttgag tttcgaccga aagaggagca atgtcgtcct gtcgcgaagg
       61 tcgatcatgg aaaaagagcg taacgaagag aaaaagaggg tcctcgagac gatcgaagaa
      121 ggaaagatcg tcgagggtac agtgaagaat atcacggatt acggaatatt cgtcgaccta
      181 ggcggtatcg acgggcttct tcacgtgacc gacatttctt ggggaaagat cacgcggccg
      241 tcggacagtt tttcaaaggg tgataagatc acggtcaagg tgctttcctt cgaccgggca
      301 aaagagaggg tctcgctcgg gctgaagcag ttgaaggaaa acccttggga taccatagac
      361 gaaaaatacg ccgtcggctc cgtcgtggag ggaaaagtcg tcagcatcat ggaatacggg
      421 gcattcgtgg aattagaacc cggcgtggaa ggccttatcc acgtatcgga gatgttctgg
      481 acgtccagaa aatcgaaaca tccttcgaag gctgtatcag tcggagagga tgtccgcgtc
      541 atgatcctcg acgtgaacaa agaaaagaag cggatatctc tgggattgaa acaaacgact
      601 cccaacccat ggaatgcaat caaagagaaa taccccccgg gaagcatagt caagggtgtg
      661 gtgaggaaca taacggactt cggtgtcttt gtcggccttg aagagggaat cgacggactc
      721 gtgcatgttt cagatatatc ctggcgccgc aaggtcaagc atccttcgga attcttcaag
      781 aaaggacagg aagtagaggc ggtggttctg ggggtggacg tggagaacga gaagttttcc
      841 ctcggaatca aacaactcga gaggaacccc tgggatgaaa tcaccgcgaa gtaccccgtc
      901 ggttcgagcg tgtccgggcc gattactaac gtgactgatt tcggagtttt cgtcgcgatc
      961 gaagagggga ttgagggact tgtgcacata tctgaactga gccagaagaa agtgaagtcc
     1021 ctggcggaaa atttcaaggt tggcgatgtt gttacggcat cggtgaagaa cgtagacacc
     1081 aagagcaaaa aaatccggct cagcatccgc gaatacgaga actctttgaa tgagacgtca
     1141 ggtaaccagt acatgaataa tcgagaaaag gtggggtcga acctcggaaa ggtgctggca
     1201 ggggttaaac tcgatgttta agctggatga gtgattccat gaggaggcat cccttcctgg
     1261 tcgggattct gatacttctg acggtgggat tcctggtgtt cctgatcttt tttggagcga
     1321 cctcggcggt ggggaggggt ttttctccgt tcagggaccg ggtaggtgta gttccgatca
     1381 agggtgtcat catggagtcc aagtcggctg tcgacctcat aaacagatac ggggaagatt
     1441 cgagggtcaa ggcgatagta gtgcggatag actcgccggg aggcggggtc gccccttctc
     1501 aggaaatatt cgatgccctt tccgctgtga agaaaaaaaa gaaagttgtt gcatccatgg
     1561 gttcggtcgc tgcttcgggg ggttattata tcgcctgtgc tgcagaccgg atcgtggcca
     1621 atcctgggac tctgacggga agcatcggtg ccttgatgca tttcgccaac gtcgaagagc
     1681 ttctgaagaa ggttggtctt aagtctgcgg tcatcaagag cggaaaatac aaagacatcg
     1741 gagcgctgac gcgggagatg acgccggaag aaagagcgct cttacagggg gttgtcgacg
     1801 acatctacga ccagtttctt ggaaccgtat cggcttcgcg gaatatctcc gtagaagaat
     1861 tgagacctgt cgccgacggc aggatattta cgggacggca ggcgctcaaa atgaggctgg
     1921 tggacgaatt gggggatatg gaacgttccg tagacgttgc ggcaaacctc gcggggataa
     1981 agggaaagcc tgaggtcgtg tatcccaagg aaaagggcgt ctctatctgg cggttcatcg
     2041 ccgacgagat ggctttttcc atagttaacc gtttgagaga cgaggcggag aaagtcggtt
     2101 atcaaggggc gtcatggatc cccctcgtaa acagctgaaa ccatggtttg aacgttgtgg
     2161 gagttggctc gggaatgctg aaagaagaag aaacacagta cagaacgatc aaggtcgagc
     2221 cgcaggaaga ttttttggtt gtctgtctta atcgtccgaa ggaaatgaac gccctctcta
     2281 aagagatgag gtgggaattg gaaagctgtt tcagcagcct ggacgaggac gattccgtta
     2341 aagcggtcgt cgtaacggga ggggattatg tcttttccgc aggaatggac atcaaggaaa
     2401 tggaagccct gccggacggg gagatcgatg attatttcaa gtcgatcatc cggtacctga
     2461 aaaagatcat gaattccaga aagccggtca tcgcggcggt cggtgggatc gccctggggg
     2521 gtggattcaa cctggcgacg gtctgcgata taatcatcgc ctccgaaagc gccatcttcg
     2581 gacacccgga actgaaattt ggattgaacc cattgttcga ccctctgtgg cgtatcgtgg
     2641 ggatggcaaa ggcaaaggag atcgccatgc tcggtgaacc gatcggggcc atggaggcct
     2701 tgagaattgg ccttgtgaac aaggttgcac cgccggacag gttcatggac gaggctttca
     2761 cgatggccag ggagttgtcg aaacgaccgg tgaaggctct cgaggccgtt aaacgtatat
     2821 cggacatcgt gccgagactt gatagggggg ctgctctcga gcacgaattt gagata
//
LOCUS       DAOTMX010000232         2871 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_27157_length_2871_cov_3.924006, whole genome shotgun sequence.
ACCESSION   DAOTMX010000232 DAOTMX010000000
VERSION     DAOTMX010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27157_length_2871_cov_3.924006"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /gene="prs"
                     /locus_tag="P5573_06840"
     CDS             <1..561
                     /gene="prs"
                     /locus_tag="P5573_06840"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HRT27812.1"
                     /translation="DLHAGQIQGFFNIPVDNLFATPILLDYMKKTHQGDETVIVSPDT
                     GGVERARAFGKRLGANLAIIDKRREGPNESQVMNIVGNVGGRRVIILDDLIDTAGTIV
                     HAAEALLNEGAKEISACCTHPVLSGPAIERIQGSVLKELIVTDTIPLHEKAKTCSKIK
                     VLSVAGILSEAVKRIYYNDSVSSLFI"
     gene            698..1351
                     /locus_tag="P5573_06845"
     CDS             698..1351
                     /locus_tag="P5573_06845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HRT27813.1"
                     /translation="MEVLELKARIRKGAGKGVSRKLRASGFIPAVLYGPGSNSISLSI
                     QSADLVAHLRAEKGEAGFIKLVIQGEGAHQEKLSMIKELQTNPVTQSLIHADFYEIRM
                     DHKITMDIPIHLVGQPVGVKMGGELHQFKRDLKVSGLPGIMPKFIEVDITGLDIGNTL
                     RVAELKLADGVEILDSEDAQIISVVAKRAVEEEKPAEEAAPSEPEVITEKKTTEEEE"
     gene            1525..2121
                     /gene="pth"
                     /locus_tag="P5573_06850"
     CDS             1525..2121
                     /gene="pth"
                     /locus_tag="P5573_06850"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HRT27814.1"
                     /translation="MKIIAGLGNPGERYLFTRHNLGFMVADRLALSHHLSFRRKKFDA
                     LLAEGPIAGVPVILLKPQTYMNLSGKAVGPLVQFFKVDAADLVVVHDDLDLPFETLRV
                     KEGGGDGGHKGLQSVIESLGTDAFLRLRLGIGKPVTKDRVESYVLEPFGKEERDRLPA
                     LLEKACGAVEEILSSGPRSAMNKFNIRGGDPNLPEPSS"
     gene            2366..>2871
                     /locus_tag="P5573_06855"
     CDS             2366..>2871
                     /locus_tag="P5573_06855"
                     /inference="COORDINATES: protein motif:HMM:NF015019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HRT27815.1"
                     /translation="MMGKKFKKFLSVSLLTLAALLLATPLFASEAELVIPVLSDAQNH
                     LLMIGFVICFLGMAFGLYEYTQVKGLPAHKSMLDVAEIIFQTCKTYLIQQGKFLLILL
                     AFISACIVYYFGFLQHLTTDNVLLILGWTVIGILGSYGVAWFGIRMNTLANARMAFAS
                     LEGKPIKLL"
BASE COUNT          660 a          854 c          776 g          581 t
ORIGIN      
        1 gacctccacg ccgggcagat ccaggggttc ttcaacatcc ccgtggacaa cctcttcgcc
       61 accccgatcc tgctcgatta catgaagaag acccaccagg gagacgagac ggtcatcgtg
      121 tcccccgaca ccggcggcgt ggaacgcgcc agggccttcg gaaagcggct cggggcaaac
      181 ctcgccatta tcgacaagcg ccgcgagggg cccaacgaat cccaggtcat gaacatcgtc
      241 ggaaatgtcg gcggccggcg ggtgatcatt ctcgatgacc tgattgacac ggcaggaacc
      301 atcgtccatg ccgccgaagc cctcctgaac gaaggggcca aggaaatatc ggcctgctgc
      361 acacaccccg ttctctcggg cccggcgatc gagaggattc agggatcggt cctgaaggaa
      421 ctcatcgtga cggacaccat ccccctccac gagaaggcca agacctgcag caagatcaag
      481 gtgctttccg tggcgggcat cctcagtgag gccgtcaagc ggatttatta caacgactcg
      541 gtcagttcgc tgttcatata aaaaagacag gcgcaaggcc cagagcaaag aatacaaaga
      601 cggctaaagg cgtgaggcca gagaccgaaa gagcaaagaa agaatgtttg ccttctgccc
      661 tgagccgtaa gccttgagcc attacactgg aggtatcatg gaagtcttag aactcaaagc
      721 ccggatccgg aaaggggccg gcaagggcgt ttcccgtaaa ttgcgtgcat cgggtttcat
      781 cccggccgtt ctgtacggcc ccggttccaa ttcaatatcc ctgtcgattc aatcagccga
      841 tctcgtcgct cacctccgcg cggaaaaggg cgaagcgggt ttcatcaagc tcgtcatcca
      901 gggggagggg gcacatcagg aaaaactctc catgatcaag gaactccaga ccaatccggt
      961 cacacagagc ctgatccacg ccgatttcta tgaaatccgc atggaccaca agatcaccat
     1021 ggatatcccc atccatctgg tcgggcagcc ggtcggcgtg aagatgggag gcgaactgca
     1081 ccagttcaag cgcgacctga aggtttcggg tcttccgggg atcatgccca aattcatcga
     1141 ggtcgacatt acggggctcg atatcggaaa caccctgcgg gtggccgagc tgaagctcgc
     1201 cgatggcgtc gagatcctcg attccgagga cgcacagatc atttccgtcg tcgccaaacg
     1261 cgccgtcgag gaagagaagc ccgcagagga agccgccccg tcggaaccgg aggtcatcac
     1321 cgagaagaag acgacggaag aagaggagta agacaccggc tcctctctgt tgtcaccgta
     1381 ccctccttcg accccatcct ccaggcagtg atggggtttt cgatatcgag aggctgaggg
     1441 cagggacatc agaccgaaga tgaccggaat gattcccgtt ctttaaccct gagtcatgcg
     1501 ccttttgccg acgcggagcg agctgtgaag atcatcgccg gtctcggaaa tccgggtgag
     1561 cggtacctct ttacccgcca taacctgggg ttcatggtgg ccgaccggct tgccctctct
     1621 caccacctca gcttccgacg aaaaaaattc gacgccctcc tcgccgaggg acccatcgcg
     1681 ggtgtccccg tcattctgct caaaccccag acctacatga atctgagcgg aaaggccgta
     1741 ggccccctgg tgcagttttt caaggtcgac gccgccgacc tcgtggtcgt ccatgacgac
     1801 ctggacctgc ccttcgagac ccttcgcgtc aaggaaggcg gaggcgatgg cggtcataag
     1861 ggactgcagt ccgtcatcga gtcactgggg acggacgcct ttctccgcct caggctcggc
     1921 atcggaaagc ccgtcacaaa agaccgcgtg gaatcctacg tcctggaacc cttcgggaaa
     1981 gaagaaagag accgccttcc cgcactcctc gagaaggcct gcggggccgt ggaggaaatc
     2041 ctttcctcag gaccccggtc ggccatgaac aaattcaaca tccgcggcgg cgaccccaac
     2101 cttccagagc cgtcgtcatg aacccccggg aaaacccgcg agaaggggcc ttttcccccc
     2161 gttcatttac cacggcatga caattgttaa cggcttgaat tttaatgata tttcatcatt
     2221 ccatccgtcc cgtcgcgccc ttccctcccc tgtgataaaa aactttgaca aacggggtaa
     2281 tatctgttat gtcacggcgg cttcagacgg ggacaagaaa tggtttcatt tctcaggtga
     2341 taaataattt ttcaaaggag aagggatgat gggtaaaaaa ttcaagaaat tcttaagtgt
     2401 gagcctcctt acgctcgccg ctttgctcct cgcgacgcct ttatttgcga gtgaagcgga
     2461 actggtgata ccggttctga gtgacgcaca gaaccattta ctcatgatcg gatttgtcat
     2521 ttgcttcctg ggcatggctt tcggcctcta cgagtatacg caggtcaagg gtctgcccgc
     2581 ccacaagtcc atgctggatg tggccgagat cattttccag acctgcaaga cctacctgat
     2641 tcagcagggg aaattcctcc tcattctttt ggctttcatc agtgcctgca ttgtctatta
     2701 cttcggtttc cttcagcatc ttacaacgga caacgtgctg ctgattctgg gatggacggt
     2761 gatcggcatc ctggggtcct acggtgtcgc ctggttcggc atccggatga acaccctcgc
     2821 caatgcccgc atggcctttg cctccctcga aggcaagccc atcaaactgc t
//
LOCUS       DAOTMX010000233         2846 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_27483_length_2846_cov_3.649946, whole genome shotgun sequence.
ACCESSION   DAOTMX010000233 DAOTMX010000000
VERSION     DAOTMX010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27483_length_2846_cov_3.649946"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="P5573_06860"
     CDS             complement(<1..235)
                     /locus_tag="P5573_06860"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HRT27816.1"
                     /translation="MIFTQEQLEKYADVLLWGLKTARTGSYKKGDIVLVQGDLAAISL
                     AEALQPKLLDMGMNVVVRIGGTPRMERTFYEKTN"
     gene            complement(270..860)
                     /locus_tag="P5573_06865"
     CDS             complement(270..860)
                     /locus_tag="P5573_06865"
                     /inference="COORDINATES: protein motif:HMM:NF014886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase alpha/beta domain-containing
                     protein"
                     /protein_id="HRT27817.1"
                     /translation="MVRKHTNTDVTCRYFHQTGKENTKPLLEVAARRAGELAIGKVIV
                     ATCSGWTAFAAIEAFPPETKIVAVTHYAGFEKPNLQELSAANRRKLESLGVSVLTCQH
                     AFGGVGRGIRRKLATYQVDEIIAYALRTFGQGTKVAIEIALMAADAGLVRTDEDVVAI
                     GGTAEGADTALVLQPANSSDLFDLKVREVIAKPKIF"
     gene            complement(918..1538)
                     /locus_tag="P5573_06870"
     CDS             complement(918..1538)
                     /locus_tag="P5573_06870"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRT27818.1"
                     /translation="MNKKKLFFVCIAVFPFMICTGIVYSILSLYMAEVGLSKSQIGFL
                     YTSGAVAGAVGSPFLGGLADRFGRKKILLLSMGGFAAVFTGYALARSYIPLFVIQVGE
                     GISWAAMAAATTALVADLSPQEHRGKAMGIYNMTWNMGWIIGPSMGGILSDRMGFTFT
                     FKLCTCLTVLGLVLALFFVPGKGRGTGGSGRTESSDIAEFDEMRGK"
     gene            complement(1811..2002)
                     /locus_tag="P5573_06875"
     CDS             complement(1811..2002)
                     /locus_tag="P5573_06875"
                     /inference="COORDINATES: protein motif:HMM:NF024851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HRT27819.1"
                     /translation="MKVYVDGELCIGCEACVDVCPEVFEMQDDVAVVRIEDDVPEELE
                     ASVEEAAESCAVEAIIIED"
     gene            complement(2205..2624)
                     /locus_tag="P5573_06880"
     CDS             complement(2205..2624)
                     /locus_tag="P5573_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HRT27820.1"
                     /translation="MKSYREELWFDIPSRRAFVNITDRVEDCLRKSGIQEGLVLVNAM
                     HITASVFINDDEPGLHQDYDEWLEKLAPHEPVSRYRHNRTGEDNGDAHLKRQVMGREV
                     VVAVTKGSLDFGPWEQIFYGEFDGRRRKRVLVKIIGE"
BASE COUNT          591 a          867 c          752 g          636 t
ORIGIN      
        1 ggttggtttt ttcgtagaaa gtgcgctcca tccttggagt gccgcctatg cgaacgacca
       61 cgttcatccc catgtccagc agcttcggct gtaaggcctc tgcaaggctt atagcggcaa
      121 ggtcgccctg gacgaggacg atgtctccct tcttgtagct gcccgtccgc gcagtcttca
      181 aaccccacaa caacacatcc gcgtacttct ccagttgctc ctgcgtaaaa atcattcggc
      241 acctccatcg gcaatgtgaa tggcgaatat taaaatattt tgggttttgc gatgacctcc
      301 ctcactttca agtcaaagag gtccgagctg ttcgccggct ggagcacgag ggccgtgtcg
      361 gccccttccg ccgtgccgcc aatcgccacc acgtcttcat cggtccttac caggcctgcg
      421 tcggcggcca taagggcgat ctcgatcgcc accttcgtcc cctggccgaa ggtccgcagg
      481 gcataggcga tgatctcgtc gacctggtac gtggcgagtt tcctcctaat gccgcgtccc
      541 acacccccga aggcatgctg gcaggtgagg acggaaaccc cgagggactc gagtttccgc
      601 cggtttgctg cgctcagctc ctggaggttc ggcttttcga aaccggcgta atgagtgacg
      661 gcgacgatct tcgtctccgg ggggaatgct tctatcgctg cgaaggccgt ccagccggag
      721 caagtggcga cgatgacttt cccgatggcg agttctccgg ccctgcgggc cgcgacctcc
      781 aggagaggtt tcgtgttctc cttccccgtt tgatggaaat accgacatgt cacatcggta
      841 ttggtatgct ttcggaccat ggacacaatc tcctcgataa atttccgggt ctttgatcga
      901 taggcgtttc tataccatca tttacctctc atttcatcaa attctgcgat gtcggacgac
      961 tccgttcggc cgcttccgcc ggtccctcga ccttttccgg ggacgaaaaa gagcgcgagg
     1021 acgaggccga gcacagtcag gcaagtacaa agtttgaagg tgaaggtgaa ccccatgcgg
     1081 tcggagagga ttccccccat ggatggcccg atgatccaac ccatgttcca tgtcatgttg
     1141 tagatgccca tcgccttgcc gcggtgctcc tgcggcgaaa ggtcggctac cagggcggtc
     1201 gtcgccgctg ccatggcagc ccaagagatc ccctctccga cctggatgac aaaaagggga
     1261 atataacttc gggccagggc gtacccggtg aatacggcgg caaagccccc catcgagagg
     1321 aggagtatct ttttccggcc gaaacgatcg gcgaggccac cgagaaaagg ggaacctaca
     1381 gcgccggcaa cagcgccgct cgtgtaaagg aagccgatct gactcttgct cagcccgacc
     1441 tcggccatgt acaaggaaag gatggagtac acgatccccg tacagatcat gaacgggaag
     1501 accgctatgc agacgaaaaa gagtttcttt ttgttcatta tttacgcccc tttcccccgc
     1561 tgtcggccgc aatagcaccg gttttacgct gccgccgagg taacaacttc cccttgctca
     1621 ccttgccggc aacgggttct tcattcccac gccatgaacg attctttcgc gggccgtcgc
     1681 ggccggagac tgtaaaaagg gataaggtca gggggaaaaa gaagggcccc ttcagccccg
     1741 ccgggctgaa gggcaagtaa gatttaatgc cgcaaacggc ctgcgctccc gatacggcgg
     1801 tcttacgaaa tcagtcctcg atgatgatcg cctcgacggc gcaagactcg gccgcttctt
     1861 ccaccgaagc ttccagttct tccgggacat catcctcgat tcttaccacg gctacatcgt
     1921 cctgcatctc aaaaacctcc gggcagacat ccacgcaagc ttcgcatccg atacagagtt
     1981 ctccgtcgac gtatactttc atgaccgtcc ctttcttccg atcaccggat gaactgtcac
     2041 cgtcccccca cgccccctcg cggcatcgga cggtttcagg cctcgaatct gcaatcattt
     2101 gacaatgatg tcagtcccat agtttaaagc tcgcagcttg tcaagaagga atttttgtcc
     2161 gccggcgaga cccatcaagt cccgcggtga ccagaaaagg gcatctattc cccgatgatt
     2221 ttcaccaaga cccgctttct cctccttccg tcgaactcac cgtagaaaat ctgctcccag
     2281 ggaccgaagt cgaggctgcc tttcgtgacg gcgacgacaa cctcccgccc catgacctgt
     2341 cgcttaaggt gggcgtctcc gttgtcctcc cccgtgcggt tgtggcggta acgggagacg
     2401 ggctcgtggg gcgccagttt ctcgagccat tcatcgtaat cttggtgcag tcccggctcg
     2461 tcgtcgttga taaaaaccga tgccgtgatg tgcatggcgt tcacaaggac aaggccttcc
     2521 tgtatgccgc tctttctcag acaatcctcg acccggtccg tgatgttgac gaaggcccgc
     2581 cgcgacggga tgtcgaacca cagttcttcc ctgtaagatt tcatcgctca cctcctcggt
     2641 gtttttcggc gcgaagttta gcagggaacc ggctggaggg gaagagcgtt ctgcctaaca
     2701 cagcgtctcg ccgcagttgc aaaaaaaccg aaaattagat atttcctttt gccagtcctt
     2761 cagtagagga gtccttccat gaatagtgag gtcgtcaagg caaacatcgt agtcttcata
     2821 gcaagcttct gcacgctggt catcga
//
LOCUS       DAOTMX010000234         2837 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_27608_length_2837_cov_3.180446, whole genome shotgun sequence.
ACCESSION   DAOTMX010000234 DAOTMX010000000
VERSION     DAOTMX010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27608_length_2837_cov_3.180446"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1578
                     /locus_tag="P5573_06885"
     CDS             <1..1578
                     /locus_tag="P5573_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HRT27821.1"
                     /translation="EGSPVTAVQIGDPITQKKMTDFLLLARDRDLYRAITDNGAGGLS
                     SSVGEMARLSGGCELDLAKAPLKYAGLQPWEILLSEAQERMTLAVEPEKADAFLSLAR
                     KMGVEATVLGRFTDSGRFHVLYGDKTVAYLDMAFLHDGVPQLELKARWVAPRHEEAAF
                     PEPSDLGGALKTMLSRLNICSKESVVRQYDHEVQGGSVIKPLTGAVNDGPSDAAVIRP
                     ILDSFEGVIVTNGICPRYSDIDTYHMAACAIDEAVRNTIAVGGSLGNIAGLDNFCWCD
                     PVQSEKTPDGEYKLAQLVRANRALYDYTRAYGVPCISGKDSMKNDYHVGDTKISIPPT
                     LLFSTVGRIADVRKAVTMDAKAAADIVYVLGETFAELGGSEWYALHGCVGNNVPKVNA
                     EKAMRLYDALSRAVEAGTVASCHDCSDGGLGVALAETAFSGGLGMRVDLARVPCEGIE
                     RNDELLFSESQSRFVVTVSPEKKEAFEAVMEGNVYAEIGVVLPEHLFVVTGMTGGKVI
                     EEDIYELKEAWQKTLRF"
     gene            1612..2439
                     /locus_tag="P5573_06890"
     CDS             1612..2439
                     /locus_tag="P5573_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HRT27822.1"
                     /translation="MARKVRTIVLTGNGTNCEVETAHACRLAGSDEVDIVHISELLYG
                     EKRLDDYHFLNLPGGFLDGDDLGSAKAGANRILHAVVSGRNERLADQFFRFIREGKLI
                     LGVCNGFQLMVKLGILPAFDGDYRSQVTTLTFNDSGRFEDRWVYLRVQEDTPCVFTRG
                     IGSLYLPVRHGEGKFVTKSRQVLKKLYRQNQVVLQYSDGSCLAPTMDYPANPNGSTDA
                     IAGICDETGRLFGLMPHPEAYHHRTNHPRWTREGLPEEGQGLVLFRNAVAFIQSADF"
     gene            complement(2458..>2837)
                     /locus_tag="P5573_06895"
     CDS             complement(2458..>2837)
                     /locus_tag="P5573_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416741.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HRT27823.1"
                     /translation="YPVIDLLPEQRHITDRGATMRLGSYPCVLENSSFAFEAYGTKEI
                     QERHRHRYEFNNEYRDLLVKSGLFISGTSPEGRLVEIAEVKDHPWFLGCQFHPEFKSR
                     PLKPHPLFVKFIEASLANRRRKG"
BASE COUNT          550 a          863 c          923 g          501 t
ORIGIN      
        1 gagggttcgc cggtgacggc cgtccagatc ggggacccca taacacagaa gaagatgacg
       61 gactttcttc tccttgccag ggaccgggac ctctaccggg cgatcacgga caacggcgcg
      121 ggcgggcttt cctcttccgt gggggagatg gccaggctct ccggcgggtg tgaactcgac
      181 ctggcgaagg cgcccctgaa gtacgcgggg cttcagccct gggagatact cctttccgag
      241 gcccaggaga ggatgaccct cgccgtggaa ccggaaaagg ccgacgcctt cctctcccta
      301 gcgaggaaga tgggggtcga ggcgaccgtc ctcgggaggt tcaccgattc ggggaggttc
      361 catgtcctct atggggataa gacggtggcg tacctggaca tggcgttcct ccacgacggg
      421 gtgccgcagt tggagcttaa ggcccgctgg gtcgccccgc ggcacgaaga ggccgccttt
      481 cccgagccgt cggacctggg aggggccttg aagacgatgc tctcccgcct aaacatctgc
      541 agcaaggagt cggtggtgcg gcagtacgac catgaggtcc agggggggag cgtcataaag
      601 cccctcacgg gcgccgtgaa tgacgggccg agcgacgccg ctgtgatccg gccgatcctg
      661 gactccttcg agggggtcat cgtcaccaac gggatctgcc cccgctacag cgacatcgac
      721 acctaccaca tggcggcctg cgcgatcgac gaggccgtga ggaacacgat tgccgtcggc
      781 ggttctctcg gaaatatcgc cggcctcgac aacttctgct ggtgcgaccc cgttcagtct
      841 gaaaagactc ccgacggcga gtacaagctc gcccagctcg tccgggcgaa ccgggccctg
      901 tacgactaca cccgggccta cggcgtcccc tgcatctccg ggaaagacag catgaagaac
      961 gactaccatg tcggggacac gaagatctcc atcccgccga cgcttctctt ctcgacggtc
     1021 gggcggatcg ccgacgtgcg gaaggccgtg acgatggacg ccaaggcggc ggcagacatc
     1081 gtgtacgtcc tgggagagac cttcgccgaa ctcggtgggt cggaatggta cgccctccac
     1141 ggctgcgtgg gtaacaacgt cccgaaggtg aacgcggaga aggcgatgag gctgtacgac
     1201 gccctgagcc gggcggtcga ggccggcacg gtggcctcct gccacgactg ttccgatggc
     1261 gggctcggcg tcgccctggc ggagacggct ttctcaggcg gcctggggat gagggtagac
     1321 ctcgcccgcg tgccctgtga aggcatcgaa cgcaacgatg agctcctctt ctccgagtcc
     1381 cagagccggt ttgtggtgac cgtttcgccg gagaaaaaag aggccttcga ggccgtcatg
     1441 gaggggaacg tctacgccga gataggggtg gtcctcccgg agcacctctt cgtcgtgacc
     1501 gggatgacgg gcggaaaggt tatcgaggaa gacatctatg agctgaagga ggcgtggcag
     1561 aagacgctta ggttctgagc gcccgtctta tccggggaga gagtgattcc gatggcaaga
     1621 aaagttagga cgatcgtgct cacgggcaac gggacgaact gcgaggtcga gacggcccac
     1681 gcctgccgtc tcgcaggttc cgacgaggtg gacatcgtac acataagcga gctgctctac
     1741 ggcgagaagc gtctggacga ctaccacttc ctcaacctcc ccggcgggtt cctcgacggc
     1801 gacgacctgg ggtcggccaa ggccggcgcc aaccggatcc tccacgccgt cgtctccggg
     1861 aggaacgaga ggctcgcaga ccagttcttc cgcttcatcc gcgagggaaa gctgatcctc
     1921 ggtgtgtgta acggttttca gttgatggtc aagttaggga tcctgccggc cttcgacggt
     1981 gattatagaa gccaggtaac gaccctcacc ttcaacgact cgggccgctt tgaagaccgc
     2041 tgggtctacc tgcgggttca agaagatacg ccttgtgtct tcacccgcgg catcggcagc
     2101 ctctacctcc ccgtccgcca cggcgagggg aagttcgtca ccaaaagcag gcaggtactt
     2161 aaaaagctct accggcagaa ccaggtcgtc ctccagtaca gtgacgggag ctgcctcgcc
     2221 cccacgatgg attacccggc aaaccccaac ggctctacgg acgcgattgc cgggatctgc
     2281 gacgaaacgg gccgcctctt cgggctgatg ccccatcccg aggcttatca ccaccggacg
     2341 aaccacccgc gctggaccag ggaaggactg cccgaagaag gccagggatt ggtcctcttc
     2401 cggaacgccg tcgccttcat ccagagcgcc gatttctgaa caactgaaaa acgcccctta
     2461 gcctttacgg cgccggtttg cgagcgacgc ctcgatgaac ttcacgaaca ggggatgggg
     2521 tttcagcggc cgggacttga actctgggtg aaactggcat ccaagaaacc agggatggtc
     2581 tttgacctcg gcgatctcga cgagacgccc ctccggcgag gtcccgctga tgaagaggcc
     2641 gctcttcacg aggaggtcgc ggtattcgtt gttgaattcg taccggtgcc ggtggcgctc
     2701 ctgtatctct ttcgttccgt aggcctcgaa ggcgaaggag ctgttttcga ggacgcaggg
     2761 gtaagacccc aggcgcatcg tcgcccccct gtcggttatg tgtcgctgtt caggtaaaag
     2821 gtcgatgacc gggtaag
//
LOCUS       DAOTMX010000235         2836 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_27619_length_2836_cov_3.893204, whole genome shotgun sequence.
ACCESSION   DAOTMX010000235 DAOTMX010000000
VERSION     DAOTMX010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27619_length_2836_cov_3.893204"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            437..694
                     /locus_tag="P5573_06900"
     CDS             437..694
                     /locus_tag="P5573_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27824.1"
                     /translation="MYFMMICTWEPKDEKEVRTRRANWEWPKDIKILFEFYDLQGCRT
                     VYVLESDAVGLIAARAAWFDIIKFEIFPVYPIGETKKMLKK"
     gene            746..916
                     /locus_tag="P5573_06905"
     CDS             746..916
                     /locus_tag="P5573_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27825.1"
                     /translation="MSKLDLTPDEEKELLAVLERYRHDLRIEIANTDDREFRKFLRKR
                     EEFINDLIDRLK"
     gene            1033..2127
                     /gene="ychF"
                     /locus_tag="P5573_06910"
     CDS             1033..2127
                     /gene="ychF"
                     /locus_tag="P5573_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003026825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HRT27826.1"
                     /translation="MGFRCGIIGLPNVGKSTIFNALTAAGAEVANYPFCTIDPNIGIV
                     RVPDKRLEKIAGIIKPPKVTYTTMEFVDIAGLVKGASRGEGLGNKFLGHIREMDALVH
                     VIRCFDNPDVVHLDGDVDPERDIGIVNMELVLADLETVERRFQKAEKMAKTGDKNAAQ
                     ELHFYRRLRDALGKGIPARKTPVEEEFKELMRELHLLTAKKMLYVANVSEDVLRKGNG
                     YIRQVEDCAKRDDSPSIVICGDLEAEMTELSEDERAAFLKDLGLDESGLDKLIRSGYG
                     MLDLITFYTTVGTELRAWTVEAGTRAPQAAGKIHSDMERGFIRAEVVHYDDFINSGGI
                     QAAKERGLLHSEGKDYQIKDGDIVLFRFNV"
     gene            2239..2607
                     /locus_tag="P5573_06915"
     CDS             2239..2607
                     /locus_tag="P5573_06915"
                     /inference="COORDINATES: protein motif:HMM:NF008448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajD family HNH nuclease"
                     /protein_id="HRT27827.1"
                     /translation="MKRDLQKPPGEDYREKSLAIHGLICARCAREFDESNRHLLTVHH
                     KDGNHMNNPLDGSNWENLCVYCHEDIHGRGLLGDFFDGSEGRKEEAVVYTDDKESSEG
                     VLGKKLIRALREKEEKKRRR"
BASE COUNT          767 a          615 c          807 g          647 t
ORIGIN      
        1 gcagcggtct gcaaaaccgt tattcaccgg ttcgaatccg gttgccgcct ccagagagta
       61 tattgaaagg gttacggcct aggtagctga taaccctttc atttttccgg cacttcgcat
      121 cctttcatag tcatgcacac ccagacaaac caataaagcc agcgttgata ggtggaatgg
      181 gttttccccg gctaacatca ggatttcttt tttgggcctc tttgtgctaa tataacaaca
      241 aaagatactg ttgaaataat aagtctcaac cggggattct taatcatgag cagcctactc
      301 attcaaaccg gcaatcgaag gaggggttat gaaaggtcgg tttcttaagt taccctcagt
      361 atctgtgcgg aagggttccc ttccgggttt gtggcaagtc gctgttgttt atcagactta
      421 agaaggaggt gattttatgt acttcatgat gatctgcacc tgggaaccga aggacgaaaa
      481 ggaggtcagg actaggaggg caaactggga gtggccgaag gatatcaaga ttctttttga
      541 attttacgat ttgcagggct gcagaaccgt ttatgtactg gaatccgatg ctgtcggctt
      601 gatcgctgcc cgtgcggcat ggttcgatat catcaagttt gagatattcc ctgtttatcc
      661 gatcggggag acaaagaaga tgctgaaaaa gtaacgtacg acgcgaccat gcacgctacc
      721 tttcaagaga aaggaggtat caatcatgtc gaaacttgac ttgacgccgg atgaggagaa
      781 ggagcttctg gcggtccttg agcggtatcg ccacgatctg cgaatcgaga ttgcgaatac
      841 cgacgacagg gaattcagaa agttcctcag aaaaagagaa gaattcataa atgacctgat
      901 cgatcgactg aaatgatcag gccgaaagag gcgaacatgg gccggctgat gttgtcggcc
      961 cttttttaga ggttgttttg aaagttacaa aaaatctgct attgctcaac cagcccgtga
     1021 ccggaggaac gcatggggtt tcgctgcggc atcatcggcc ttccaaatgt cggaaaatcg
     1081 acgattttta atgccctgac agcggcaggc gctgaagtgg ccaattatcc attctgtact
     1141 atcgacccta acatcggtat cgttcgcgtt cctgacaaac gtcttgagaa aatcgccggc
     1201 atcatcaagc cgcccaaggt cacatacacg acgatggaat ttgtggacat cgcgggcctt
     1261 gtgaaagggg ccagccgagg ggaaggtttg ggaaacaagt ttctggggca cattcgcgag
     1321 atggacgctc tggttcatgt cataaggtgt ttcgacaacc cggacgtggt tcacctagac
     1381 ggggacgtag acccggagcg ggatatcggc atagtgaata tggagctcgt cctagccgac
     1441 ctcgagaccg tggagaggag gttccagaag gccgaaaaaa tggcgaagac aggcgataaa
     1501 aacgccgcac aggagctgca tttctatcgg cgcctcaggg atgcgctggg gaagggcatt
     1561 ccggcaagaa aaacacctgt ggaggaagaa ttcaaggagt tgatgcggga actccatctc
     1621 ttgacggcaa agaagatgct ctatgtggcc aacgtgagcg aagacgtgct gaggaaggga
     1681 aacgggtata tcaggcaagt tgaggactgt gctaagaggg acgattcccc gagcatcgtc
     1741 atctgcggcg accttgaggc ggaaatgact gaattgtctg aagacgaaag ggccgctttt
     1801 ctcaaagacc ttggactcga tgaatcggga ctcgacaagc tgatcaggtc cgggtacggt
     1861 atgcttgacc ttatcacttt ttatacgaca gtaggaacgg aactgcgagc ctggacggtt
     1921 gaggccggca cgagagcacc ccaggcggca ggtaaaatcc attcggacat ggaacgcgga
     1981 ttcatacgag cggaagttgt gcattacgat gattttatca actcaggtgg catacaggcg
     2041 gccaaggaaa gaggccttct tcattcggaa ggcaaggatt accagataaa ggacggcgac
     2101 atcgtcctgt ttcgattcaa tgtgtgagga cggctgatgg cgagaatgac cttttcaaaa
     2161 aaaagcggcc gcgtccgagc gagcatcacg aaggatcaga cggtgcctgg aagggccgtt
     2221 gaggacatcg tcagagacat gaagagggac cttcaaaaac ctcccggcga ggattaccgg
     2281 gagaaatcat tggcgatcca cgggctgatc tgtgcccgtt gcgctaggga gtttgacgag
     2341 tcgaaccgtc atcttctgac ggtacaccac aaggacggga accacatgaa caatcccctc
     2401 gacggtagca actgggaaaa cctttgtgtt tactgccatg aggatataca tggacgcggc
     2461 cttttgggcg atttttttga tggatcggaa ggtcggaagg aagaggctgt agtctatacc
     2521 gacgataaag aatcgagcga aggtgtcttg ggaaagaagc tgatccgggc attgagagaa
     2581 aaagaagaga agaaacgcag gcgctgaaaa caggtcaccc ccaccctccc gatgagtata
     2641 tgcctttctt tttatccttg taaagcacag gcgattatgc taattaaacg tcgtttcagg
     2701 ctgtgtgcgt ggtgcttgta aggcgatcgt tatcaatatg ctattataac agccggttgt
     2761 gcttcgatat ggcctaagtg gtttagaatt cacaagtaag gaggtaaggt caggtgaacg
     2821 ttattgcaca aggaag
//
LOCUS       DAOTMX010000236         2829 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_27718_length_2829_cov_3.088320, whole genome shotgun sequence.
ACCESSION   DAOTMX010000236 DAOTMX010000000
VERSION     DAOTMX010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27718_length_2829_cov_3.088320"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /locus_tag="P5573_06920"
     CDS             complement(<1..965)
                     /locus_tag="P5573_06920"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HRT27828.1"
                     /translation="MALILPFRAVRPRREFVAEVASYPYDVISSEEAREIVKGNPLSF
                     LHVTKAEVDLPADIDPYDARVYERARQNFEEMLKKGVLFQDETPCFYVYRLRRGEHEQ
                     RGVGACVHIKDFEEGRVKKHEMTLPDKREDRRRHIDVVDAQTGPIFLTYRRREEVSRV
                     VEEIMKEKEPEYDFVSRDGVSHTLWVVKDGEDVGMLARALKDIDAMYIADGHHRAASG
                     VAVAQMRRRDGQSRGLYLPHNLILAVLFPDDELQVKGYNRVVKDLCGMDEGEFIARLE
                     KGFLVEPDYRERTPKKPHEYGMYLKGRWYRLRVKEGTYPADDDMES"
     gene            1128..1604
                     /locus_tag="P5573_06925"
     CDS             1128..1604
                     /locus_tag="P5573_06925"
                     /inference="COORDINATES: protein motif:HMM:NF024107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HRT27829.1"
                     /translation="MPVRVFIHGLESSSQGNKGVFFRERYPDMIIEDFEGPLEQRMDK
                     LNDILKDKEDLIIVGSSFGGLMAALYAFRNRAKVRKLILLAPALDLEDFDPCLSDRLD
                     IPVVIFHGKNDDVVPVAEVKNRAARVFSNLEYHLVDDDHPLRKTFTTYDWEELLRP"
     gene            1650..2063
                     /locus_tag="P5573_06930"
     CDS             1650..2063
                     /locus_tag="P5573_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIL domain-containing protein"
                     /protein_id="HRT27830.1"
                     /translation="MYTKKVVLRYPPDVVDQPIVYRLVKDYDLVFNILKARVFPRREG
                     LLVLELRGTKENFDRGIRYLKEQGLRVEALSKSVKRKEDKCVHCGACTAFCPTEALHF
                     ENGSSAKVVFDAERCNGCEICVSACPPRAMEVDLL"
     gene            complement(2067..2417)
                     /locus_tag="P5573_06935"
     CDS             complement(2067..2417)
                     /locus_tag="P5573_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27831.1"
                     /translation="MGDTVSHIFSNPLLMTILAVAGLVVVFFIVKKLFKAALILALLL
                     LIGVFLYHGFSTPGDVEQKMKGAYEKTKAQSEEVLKQGKEKVAKEGKKLTKELGRTAA
                     KKAKVFDDGGQADE"
     gene            2563..>2829
                     /locus_tag="P5573_06940"
     CDS             2563..>2829
                     /locus_tag="P5573_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HRT27832.1"
                     /translation="MKVAVATLGCKVNQFDSALLAERLRSEGFTVVPFGEGADVCIIN
                     TCTVTARTDYQSRQLVRRARRYCPYAGIVVTGCYAQVAPAELWLS"
BASE COUNT          587 a          822 c          787 g          633 t
ORIGIN      
        1 gactccatgt cgtcatcggc ggggtacgta ccttccttca ccctgagtct gtaccacctc
       61 cccttcaggt acatgccgta ctcgtggggt ttcttcggcg tcctctcgcg gtagtccggc
      121 tcgaccagga atcccttctc caatcttgcg atgaattcac cctcgtccat cccgcaaagg
      181 tcttttacga cacggttgta gcccttcact tgcagttcgt cgtcgggaaa caggacggca
      241 aggatgaggt tgtgcggcag gtaaagtccg cggctctgac cgtcccgcct ccgcatctgc
      301 gcaaccgcca ctcccgaggc tgcccggtga tggccgtcgg cgatgtacat cgcgtctatg
      361 tccttcaagg cccgcgccaa catacccacg tcctcgccgt ctttcaccac ccacagggta
      421 tgggagacgc cgtcacggga gacgaaatcg tattccggct ctttttcctt catgatctcc
      481 tcaacgaccc ggctgacctc ttcccggcgc cggtacgtca aaaagatggg cccggtctgg
      541 gcatcgacta cgtcgatgtg cctcctcctg tcctcccgct tatcgggaag ggtcatctcg
      601 tgcttcttta ctctcccctc ctcgaaatct ttgatgtgga cacaggcgcc gaccccacgc
      661 tgctcatgtt cccctctccg caagcggtac acatagaaac agggcgtttc atcctggaag
      721 agaacaccct ttttcagcat ctcctcgaag ttctgcctcg ccctctcgta gacccgggcg
      781 tcataagggt ctatgtccgc cggcaggtcg acctcggcct tcgtgacatg gagaaaactg
      841 agggggttgc ctttcactat ctctctcgcc tcttccgagg agatcacgtc atagggatag
      901 gaggcgactt ccgccacgaa ctcccggcgg ggcctcacgg ctctgaaggg taggattagg
      961 gccataaaat ctcctttttg tgggagcttg tctaacacac ggaatgatgc aaaatcaaca
     1021 tctttgcggc gaggtgtgcc atcgcaccgc cggcaaggcg gcccttgcaa aactgaggca
     1081 tccgttatat ggagcagggc gtcttaacac cacagggagg ttttgttatg cccgtcagag
     1141 ttttcatcca cggcctcgag agcagcagcc aagggaacaa gggcgttttc ttccgcgaga
     1201 ggtatcccga catgatcatc gaggacttcg agggaccttt ggaacaacgc atggacaagc
     1261 tgaacgacat cctcaaggac aaagaagact taatcatcgt cggctcgagt ttcggcgggc
     1321 tgatggccgc cctgtatgcc ttccgtaaca gggcgaaggt ccgcaagctc atcctcctgg
     1381 ctccggcgct cgacctcgag gatttcgacc catgtctaag tgaccgcctg gacatccccg
     1441 ttgtcatctt ccacgggaaa aacgacgacg tcgtgcccgt ggccgaggta aagaacaggg
     1501 ccgcccgggt cttctccaac ctcgaatacc acctcgtcga cgacgaccat ccgctgagaa
     1561 agacgttcac cacttatgac tgggaggaat tgctcagacc ttgacaggat gggctcccta
     1621 tgagagaatc ctcgaccgag gaagcaggaa tgtatacgaa aaaggtcgtc ttaaggtatc
     1681 cacccgacgt cgttgaccag ccgatcgtct atcggttggt caaggattat gacctggtgt
     1741 tcaacatcct gaaggcgcgg gtgtttccaa gacgggaagg gttgttggta ctggaacttc
     1801 gcggaacaaa ggagaatttc gaccggggca tccgttacct gaaagaacaa gggctcaggg
     1861 tcgaagcgct ctccaagagc gtcaagcgca aggaagataa gtgcgtccat tgcggggcct
     1921 gcactgcctt ctgtcccaca gaggcattgc acttcgaaaa cggctcctcg gcgaaggtcg
     1981 tgtttgacgc tgagagatgc aacggttgcg agatctgcgt atccgcctgc ccgccaaggg
     2041 cgatggaggt agatctgctg taaggtttat tcgtcggcct gaccgccgtc gtcaaagact
     2101 tttgcttttt tagccgctgt cctgcccagc tctttcgtca gtttcttgcc ttctttcgcg
     2161 accttttcct taccctgctt caggacttct tcgctctgag ctttcgtctt ttcataggcg
     2221 cccttcatct tctgttcgac atctccgggc gtcgagaaac cgtggtagag gaacacgcct
     2281 atgaggagaa ggagggcgag gatcagggct gccttgaaga gcttcttgac gataaaaaag
     2341 acgacgacaa gcccggcaac ggcaaggatg gtcatcagaa ggggattcga aaagatgtgg
     2401 ctgaccgtat cgcccatacg tagtcaccgc ctcttgtagt gggcatcatg ttaccagaat
     2461 tgaccttcaa tttctacatc ctttgcccgg ctgtccccgg ggaaattaat tgacgacgcc
     2521 ggatacgcgg ggcgggataa acatagggag agagtaacac ggatgaaggt ggcggtagcg
     2581 acactcggat gcaaggtgaa tcagttcgac tcggcgcttc tggctgagag gctccgttcg
     2641 gagggcttta ccgtggtccc cttcggggaa ggggccgacg tatgcatcat caatacctgc
     2701 accgtgacgg cgcgcacgga ttaccagtcc aggcaactcg tgcgaagggc gagaaggtac
     2761 tgtccctacg ccgggatcgt cgtgacgggt tgttacgcgc aggtcgcccc cgccgagctg
     2821 tggctgtcg
//
LOCUS       DAOTMX010000237         2825 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_27762_length_2825_cov_3.751625, whole genome shotgun sequence.
ACCESSION   DAOTMX010000237 DAOTMX010000000
VERSION     DAOTMX010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27762_length_2825_cov_3.751625"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1061)
                     /locus_tag="P5573_06945"
     CDS             complement(<1..1061)
                     /locus_tag="P5573_06945"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRT27833.1"
                     /translation="MADYKRAKWAEWADHIRSLDDPAKNFEKPEALDDLVVLDVSSRS
                     MAGCFCSSLLAELGAETIRVEPPGGDFVRTYAPHGLMKDGTGLAYLQEGRNKYHVTLN
                     LEKKEGRDMLMALAGQADILIETYPTGVMEKWKLGYEDLAKANPRLIYCSITSFGQFG
                     PESKRQQFDYDNIGQARSGVQYGTGEVLPEGKTYEEMPHAVPTKAGPWIAWSVSGTFG
                     AMGILAAVHYREVTGEGQHIDVATPEAYERLHDYALHWYYDQKVLNERFGSLDTALWL
                     YGFFPTKDGGVFLGGLRLEMWKAFVDIIGKYDEWKAEEWTTLGPFTKKDWQLKYQAMI
                     DAETSKYTSEELAQKAVEYA"
     gene            complement(1068..2396)
                     /locus_tag="P5573_06950"
     CDS             complement(1068..2396)
                     /locus_tag="P5573_06950"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRT27834.1"
                     /translation="MSKAFKGYVKNKYSRPAPLKGIRVLEVCTLLFGPAGPALLAELG
                     AEVIKIELPPAGDVTRSLTPFGWFYKEQGPMFMHINPSKYYMALDLHKEEAQKIFHEL
                     AAKSDIIEFNMRPAVVKRWNIDYETIRKINPGIIYIEKNGFGQWGQYAEEDRPSNDGA
                     AQALSGYAWTSSFPGQPPLKQGIWICDVFGAMMGEVAVLAALHHRRKTGKGQYIEMSQ
                     SENIMRAMGWVWPYQQMTGKGAEPAGNRDLCICPADTFLTKDDLFCAVAAPAPDEFKG
                     LCTAMGQPALAADKRFKDHATRLQDANALALLKIIADWARTKTAAEIEKLGLKHGFAA
                     QRVHSGRDMNEDPHRRERDFVKMIDDPLYGYYPEHDFPVMMSKSPPKNNWTVRPVGFD
                     NDFILTKILGRSEAQIAEYYEKGLIGKWKDMQGRRPPPDWDKTSGAILRR"
BASE COUNT          515 a          862 c          814 g          634 t
ORIGIN      
        1 gcgtactcca cggccttttg cgccagctcc tcgctcgtgt acttcgacgt ttccgcatcg
       61 atcatggcct ggtacttgag ctgccagtcc ttcttcgtga agggtcccag ggtcgtccac
      121 tcctcggcct tccactcgtc gtacttcccg atgatatcca cgaaagcctt ccacatttcg
      181 agccgaagcc cgccgaggaa gacaccgccg tccttcgtcg gaaagaaccc gtagagccaa
      241 agcgccgtat cgaggcttcc gaaacgctcg ttcagcacct tctgatcgta gtaccagtga
      301 agggcgtagt cgtggagacg ctcgtaggcc tccggcgtcg ccacgtcgat gtgctgcccc
      361 tcgccggtta cctcccggta atgcacggcc gccagaatac ccatggcgcc gaaggtcccg
      421 gaaacggacc aggcgatcca gggaccggcc ttcgtgggga cggcgtgagg catctcctcg
      481 taggttttcc cttccggaag gacctcgccg gtgccgtact gcacgcccga gcgcgcctgt
      541 ccgatattgt cgtaatcaaa ctgctgtcgt ttgctctccg gcccgaactg gccgaaactc
      601 gtgatggagc agtagatgag cctcgggttg gccttggcga gatcttcgta gccgagtttc
      661 cacttttcca tcacccccgt cgggtaggtc tcgatgagaa tgtccgcctg ccccgcaagg
      721 gccatgagca tgtcacgccc ttccttcttc tccaggttga gcgtcacgtg gtacttgttg
      781 cgcccttcct gaaggtaggc aagtccggta ccgtccttca tgaggccatg aggtgcatac
      841 gtacggacga agtcgccccc cggaggctcg acacggatgg tctcggcccc gagttccgcc
      901 agaagcgacg agcagaagca gccggccatg ctccgggaac tgacgtcgag cacgacgaga
      961 tcgtccagag cttccggttt ttcaaagttt ttcgccggat catccaggga tcggatgtga
     1021 tccgcccatt ccgcccattt tgctctcttg tagtctgcca tagctcatca cctccttaag
     1081 atagcgccgg atgttttatc ccagtcagga ggcggcctcc gaccctgcat gtccttccac
     1141 ttcccgatga gccccttttc gtagtactcg gcgatctggg cttcgctcct cccgaggatc
     1201 ttcgtcagga tgaagtcgtt gtcaaaaccc acgggcctga cggtccagtt attcttgggg
     1261 gggctcttgg acatcatgac gggaaaatcg tgctcggggt aataaccgta gagcggatcg
     1321 tcgatcatct tgacgaagtc ccgttcgcgg cggtggggat cctcgttcat gtccctgccg
     1381 ctatggacac gctgcgcggc gaaaccgtgc ttgaggccga gcttctcgat ctccgcggcc
     1441 gtcttcgttc tggcccagtc ggcgatgatc ttgagaaggg ccagggcgtt tgcgtcctga
     1501 agccgcgttg cgtgatcctt gaagcgcttg tcggcggcaa gcgccggctg acccatggcg
     1561 gtgcagagcc ccttgaactc gtccggcgcc ggcgccgcga cggcacagaa cagatcgtcc
     1621 tttgtgagga aggtgtccgc cgggcagata cagagatcac ggttgccggc cggctcagcg
     1681 cctttaccgg tcatctgctg gtagggccag acccacccca tggcacgcat gatgttttcg
     1741 ctctgggaca tctcgatgta ctggcccttc cccgtctttc ttcgatggtg cagcgcggcc
     1801 agaacggcga cttcccccat catggcaccg aaaacgtcgc agatccagat accctgcttg
     1861 agtggcggct ggccggggaa gctcgacgtc caggcgtagc cggaaagagc ctgggcagcg
     1921 ccgtcgttgg agggccggtc ttcctcggcg tactgccccc actggccaaa gccgttcttc
     1981 tcgatgtaga tgatccccgg gttgattttc cttatggtct cgtagtcgat attccagcgc
     2041 ttgaccacgg cgggtctcat attgaactcg atgatgtcgg acttggccgc aagctcgtgg
     2101 aaaatcttct gggcctcttc cttgtgaagg tcgagggcca tgtagtattt tgaagggttg
     2161 atgtgcatga acatcggccc ctgctccttg tagaaccagc cgaagggggt aagggagcgg
     2221 gtcacatccc ccgcgggcgg aagctcgatc ttgatcactt cggctcccag ctcggcgagt
     2281 aaggcaggac ccgccggccc gaagaggagc gtgcagacct cgaggacgcg gatacccttc
     2341 agcggagcag gccgactata cttgttcttg acgtaacctt tgaaagcctt gctcatattc
     2401 ttccctcctt aggaatttct ggaacatttc gaccgccgtg acagcggcga tcaccccgta
     2461 ctctttcaca gatgaaaaga tcctttgacg actggaatcc ccgcattcaa caattccggg
     2521 gaagcttcac ctcctttccc tgccgtgata tttttgatcc gtgactccag gtaagaaaca
     2581 aagcgtgcct gacggggcgt cttcccaaaa aggacgcgac agcggtgccc catccggctt
     2641 gatcgtgcaa tctgtttagg gttctgcatt tctcgagggg aatccgcccg gaaccggtca
     2701 ctccgcctga gggtgtgaat cagggaacat aaaggatgag ggaaggcctg tgcggttccg
     2761 gggaaagcgc tccgggtttc ctgtgatccg gatttctgaa gtctcatagc tcttccatga
     2821 cacga
//
LOCUS       DAOTMX010000238         2822 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_27812_length_2822_cov_3.561258, whole genome shotgun sequence.
ACCESSION   DAOTMX010000238 DAOTMX010000000
VERSION     DAOTMX010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27812_length_2822_cov_3.561258"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..907)
                     /gene="gap"
                     /locus_tag="P5573_06955"
     CDS             complement(<1..907)
                     /gene="gap"
                     /locus_tag="P5573_06955"
                     /inference="COORDINATES: protein motif:HMM:TIGR01534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HRT27835.1"
                     /translation="MSVRVAINGFGRVGRSIIRACLGRKDIEIIAVNTRAKPEILAHL
                     LTYDSVHGKIKAEVSLKNGSLVVDGNEIRVTNITDSIDRLPWKDMQIDVVMESTGKFR
                     KKDQCLPHIAAGAKKVIIAAPGKGIDGTFCMGVNEETYDPKKHHIISNASCTTNCLAP
                     IVKVLHDTFTVKRGLMTTVHSYTMDQRLLDGSHDDLRRARAGALSIVPTTTGAAKTVA
                     EVIPDLKGKLDGLALRVPTPNVSLVDFVAELGRNVTIQDVNGALKAAAEGKLKGIVEY
                     CEKELVSVDFTSSPYSAVVDAPLTNV"
     gene            1237..2661
                     /gene="amrB"
                     /locus_tag="P5573_06960"
     CDS             1237..2661
                     /gene="amrB"
                     /locus_tag="P5573_06960"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HRT27836.1"
                     /translation="MGGLRKSVIAGTWYPGNPGILRAYIEELFAKIPAVEVDGRLIGL
                     ISPHAGYAYSGEVAAYSYKLLRGRSFESVIIIGPSHRAFFHGSSVYDRGGFETPLGVV
                     PVDAELAARIMSRGSRLSYIPSGHSLEHSVEIQLPFLQVALGSFKFVPIVMGDQSRES
                     CADLASAIAGSISGDTLIVGSSDLSHYHSDDHARKMDYRVLKHIEDMDVEGLLADLEK
                     GSSEACGGGPIAVTMMAAEKLGANGAKVLKYANSGDVTGDRSEVVGYASAAFYVKGQE
                     GRRREGDNGEKGGLSEEEKVLLLRIARGAIESRLTGREEPQLPRVSGALNEKRGAFVT
                     LHGKGGRLRGCIGCIEGRMPLYKTVREMALAAAFNDPRFPPVSRQELDNLEIEISVLT
                     PLREIKDISEIEVGKHGIYITRGFYSGLLLPQVATQYGWDRLEFLEETCLKAGLPPTA
                     WKDKNTNIYIFSADVFGERDVKVT"
BASE COUNT          589 a          721 c          831 g          681 t
ORIGIN      
        1 tgacgttggt caggggtgcg tcgacgacag ccgaataggg gctgctcgta aagtcgacgg
       61 agaccagttc tttttcgcag tactcgacga tccccttgag cttaccctct gcggccgctt
      121 tcaaggcccc gttcacgtct tggatcgtga cgttgcggcc gagctcggcc acgaaatcca
      181 ccagcgagac gttgggtgtg ggcacgcgga gggcaaggcc gtccaatttt cccttcaggt
      241 ccggtattac ttccgccacc gtcttcgcgg cccccgtcgt agtcgggacg atggagaggg
      301 cacccgccct tgcgcgcctc aggtcgtcgt gagacccgtc gagcaaccgc tggtccatgg
      361 tgtaagagtg gacggtcgtc atcagccctc ttttgacggt gaaggtgtcg tggagaacct
      421 tcacgatggg ggcgaggcag tttgtggtgc aggaagcatt cgaaattatg tgatgtttct
      481 tcgggtcata ggtttcttcg ttgacaccca tgcaaaaggt gccgtctatg ccctttcccg
      541 gtgccgcgat gatgactttt ttggcaccgg cggcgatgtg ggggaggcac tgatccttct
      601 ttcggaactt tcccgtcgac tccatcacca cgtcgatctg catatccttc cagggaagcc
      661 tgtcgatgct gtccgtgatg ttggtgactc gtatctcatt cccgtccacg acgagcgacc
      721 cgttcttgag tgaaacctct gcctttatct ttccgtgcac cgagtcatag gtcaaaagat
      781 gggccagtat ctccggtttc gcccttgtgt tgacggcgat gatttctatg tctttgcgtc
      841 cgaggcaggc cctgattatc gaccgcccca ctctcccgaa tccgtttatg gcgactctaa
      901 cgctcatgtt gacctccgct tttctggact ttgaaacggg gccagcataa ccaggccacc
      961 gattttagtc aatcgttttt ctttctgaaa aatcgggaaa ttcgccgatt tactcttgat
     1021 tttcttgttc gatcctggta gaaaaacaac ggttatttgg tctgtcactc cattttttgt
     1081 gcagtttcac ggtagttcat gattcccttc ttaattccag aacgttatga cgtgagtcga
     1141 taaatcctaa agaaagcatc gtctgttgcg tgcgttcctg cagcgaattt tttccggttt
     1201 ttacggctta aaagacggca gatgggagat aggatcatgg gaggactgag aaaatcggtc
     1261 atagcaggca cctggtaccc tggaaaccca ggcatcctga gggcttacat cgaggaactg
     1321 ttcgccaaga tcccggcggt ggaggtggat ggtcgcctga tagggcttat ttcgccccat
     1381 gccgggtatg cttactccgg cgaagtcgcc gcttattcct ataagctgtt gaggggccgg
     1441 tcttttgagt cggtcatcat cataggtccg agccataggg ccttcttcca tggttcttcc
     1501 gtgtatgacc ggggagggtt cgaaacaccc ctgggggtcg ttcctgtgga cgccgagttg
     1561 gcggcgagga ttatgtccag gggctcgcgc ctttcataca tcccgagcgg ccattccctg
     1621 gagcattccg ttgagatcca actccccttt ctccaggtcg ccctcggaag tttcaagttc
     1681 gtccccatcg ttatggggga ccagagccga gagtcttgcg cagatcttgc gagtgcgata
     1741 gctgggtcga tctcggggga tacgcttatc gtgggcagtt cagacctctc ccactaccac
     1801 tccgacgatc acgctaggaa gatggactac cgggtgctga agcacatcga agatatggat
     1861 gtggaaggcc tgctcgccga cttggaaaag gggtcttcgg aggcctgtgg aggaggaccg
     1921 atagcggtga cgatgatggc ggcggagaaa cttggggcga acggagcgaa ggtcttgaag
     1981 tacgccaatt cgggagacgt taccggcgac cggagcgagg tcgtagggta cgcctcagcc
     2041 gctttctacg tgaaggggca ggagggccgg cggagagaag gcgataacgg tgaaaaaggg
     2101 gggctttcgg aagaagagaa ggtgcttctg ctcaggatcg ctaggggcgc gatcgagagt
     2161 cgcctgacgg ggcgggagga gccgcaactg ccgcgggtgt ctggtgcctt gaacgagaag
     2221 aggggggcct tcgtcaccct gcacggcaag ggaggccgct tacggggttg catcggctgt
     2281 atagaaggtc gtatgcccct ttacaagacg gttcgtgaga tggccctggc cgcagctttc
     2341 aatgacccgc ggtttccgcc tgtaagccgg caggaactcg acaacctgga aatagagatt
     2401 tccgtgttga cccctctgag agagattaag gatatcagtg agatagaggt cggtaagcat
     2461 ggaatttaca taaccagggg gttttattcc ggtctcctac ttccgcaggt cgccacccag
     2521 tatgggtggg accgccttga atttctggaa gaaacatgtc tcaaggcagg gctccctccc
     2581 acggcctgga aggacaagaa caccaacatc tacatttttt cagcggatgt ttttggggaa
     2641 agggacgtca aagtcacata ggccggaaga aagaaataaa aagttcttga caaaaagaaa
     2701 cagaactttt aagtttccaa gttccttttt agaacactgc ttaggcatgg tcgcactgcg
     2761 gtcatggttg acgtccccgt aaaaagtcca aaaacgtcat gccggacttg atccggcatc
     2821 ca
//
LOCUS       DAOTMX010000239         2821 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_27826_length_2821_cov_3.512292, whole genome shotgun sequence.
ACCESSION   DAOTMX010000239 DAOTMX010000000
VERSION     DAOTMX010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27826_length_2821_cov_3.512292"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /gene="speB"
                     /locus_tag="P5573_06965"
     CDS             <1..606
                     /gene="speB"
                     /locus_tag="P5573_06965"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HRT27837.1"
                     /translation="VQKKIAEIFAAGKIPVMLGGEHSISVGAVRAARERFPGLSVLHL
                     DAHADMRDSYQGTPFSHACAARRIAETCPLVQAGVRSLSLEEALFLKESGIRTYSAET
                     VNERMPEVVEKICGGLSDDVYITLDLDVLDPSVMPATGTPEPGGIQWWDIIKILKTVS
                     GRRRVRGFDVVELLPIPGVVAPDFLAAKLVYRMMGYVLERG"
     gene            669..1223
                     /locus_tag="P5573_06970"
     CDS             669..1223
                     /locus_tag="P5573_06970"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HRT27838.1"
                     /translation="MKLREFVPRKIFFTKGVGTHKDELHSFERALRDAGIEKYNLVQV
                     SSIFPPGCKMISRVQGLKELVPGGITYCVMSRCCSNEPKRLLAASVGCAIPADKNAYG
                     YISEHHAFGQTEKQAGDYAEDLAAAMLASTLGIDFNVDESWDEKKEIFKISGKIVRTR
                     NVTQSTIVKNGGYTTVIAAAVFVF"
     gene            1313..>2821
                     /locus_tag="P5573_06975"
     CDS             1313..>2821
                     /locus_tag="P5573_06975"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HRT27839.1"
                     /translation="MNIYLVPPALGFVVFLCLVLAALFKGSRRPAELLFAGFCLSGAL
                     INLDIILVSAIPDKATALQVDRTIYIFFVFSIPLYIRFIHVFLGESRPWLDGAALAVS
                     ILFLVFTRSDYFISGLNEYSFGRIARAGPLYHAFCFAGAMASVYCLVMLFKALRETEE
                     GRQRNRLKFIIVGMGMSAFLLLLNYLPINGFSIYPMGNFVFVPAVIMAFGVLKYDLFD
                     VDAAIRKGMVYFFLTGTIMVIYAFLFYILDIFFREFARSHILFSPFVLALTVIFLFDP
                     VRKGVERFTDKHFFSGKYDYRRTLKEISEEMTLLLRYEDIRRFLVTSIENTVQVEDVR
                     LVVPRDDDERSRAVWGNRVIVDVLERQKAPISKGSSVISRLADGERKAFERLFEVCGA
                     VILFPVIFRGRLNGLITLGPKKSGELFVQEDLELLSTVANQCAVALENARAYEELQEL
                     NANLEGKVRERTAELERALREKENAQNRLIQSERLAAIGQLAAGVAHEINNPL"
BASE COUNT          614 a          724 c          832 g          651 t
ORIGIN      
        1 gttcagaaaa agatagcgga aatctttgct gccgggaaga ttccggtgat gcttgggggt
       61 gagcacagca tatctgtcgg ggctgtcagg gcggcgagag aacggttccc ggggctctcc
      121 gtgttgcacc tcgacgccca tgcggatatg cgggactcct atcagggaac gcccttcagt
      181 cacgcctgtg ccgcccggcg gatagcggaa acatgtcccc tggttcaggc tggggtcagg
      241 agcttgagcc tcgaagaggc ccttttcctt aaggagagcg gaatcaggac ctattcggca
      301 gaaaccgtta atgagcggat gccggaggtc gtggaaaaga tatgtggtgg cttatccgac
      361 gatgtctata tcaccctgga cctcgatgtc ctggaccctt cggtgatgcc ggccacgggg
      421 accccggagc cgggcggtat tcaatggtgg gatatcatca agatactgaa aacggtgtcg
      481 gggaggcgcc gggttcgtgg tttcgacgtt gtcgagctct tgcccattcc tggtgtcgtc
      541 gctcctgatt ttctcgcggc gaaactcgtt taccggatga tggggtatgt tctggaaaga
      601 ggatgatcaa ggtagagggt taggtgacca ccttgtttga tacagcaaag aagcgaagcg
      661 acaggagtat gaaattgaga gagttcgtgc ccagaaagat attctttacg aaaggcgtcg
      721 gtacccacaa ggacgaactt cattccttcg agcgcgccct gagagatgcg ggcatcgaga
      781 aatataatct tgtacaggtt tcgagcatat tccctcccgg gtgtaagatg atctcgaggg
      841 ttcagggatt gaaagaactc gtaccggggg gtataaccta ttgcgttatg agccgctgct
      901 gcagcaatga accgaagcgg ctcttagcgg cgtccgtggg atgcgccatt ccggcggaca
      961 agaacgccta tggttatata agtgagcacc atgccttcgg acaaacggag aagcaggcgg
     1021 gggattacgc cgaggacctc gcggccgcta tgcttgcgtc aacccttgga atagacttca
     1081 acgtggacga aagctgggac gagaagaagg aaatcttcaa gatcagcggc aagatcgtcc
     1141 gaacccggaa cgtaacccag tccaccatcg ttaaaaacgg cggttatacg acggtcatcg
     1201 cagccgccgt gttcgttttc tgaagaaaac gacaagcccc gctgcctctc cgtaagctgc
     1261 tcttcattgc tgtcattatc atccggcctc aaggctggaa ggcagtggat gtatgaacat
     1321 ctatttggtt ccccctgccc tcggattcgt ggtcttcctc tgtctggtgc tggcggccct
     1381 tttcaaggga agccgaaggc ccgccgaact tctattcgcc gggttctgtc tttcgggagc
     1441 cttgatcaat ctcgacatca ttcttgtctc agccatcccc gacaaggcga cggcgctgca
     1501 ggtggacagg accatctaca tttttttcgt cttcagtatc cccctctaca tccgcttcat
     1561 ccatgttttt ttaggagaat cgagaccttg gttggatgga gcggccctgg cggtcagcat
     1621 cctcttcctc gtcttcaccc gcagcgatta cttcatcagc ggcctgaacg aatacagttt
     1681 cggcaggatc gcaagggcgg gacccctgta ccatgccttc tgcttcgcgg gcgccatggc
     1741 gtcggtctac tgtttggtca tgctcttcaa ggccctgagg gaaaccgagg aaggcaggca
     1801 gaggaaccgg ctcaagttta tcatcgtcgg gatgggtatg agcgccttcc tgctcctatt
     1861 gaactacctg cccataaacg gcttcagcat ttatccgatg ggaaattttg tctttgtgcc
     1921 ggcggtcatc atggccttcg gcgtcctcaa gtacgacctt ttcgatgtag acgcggcgat
     1981 ccgtaaaggg atggtctact tcttcctcac cgggacgatc atggtgatct atgcatttct
     2041 gttttacatc ctcgacatct tcttccggga attcgcaagg agccatatac tattttcgcc
     2101 cttcgtcctt gccctgaccg tcatctttct tttcgacccg gtgagaaagg gtgtcgagag
     2161 gtttaccgac aagcattttt tctcggggaa gtacgattac cgcaggaccc tcaaggaaat
     2221 aagtgaagaa atgacccttc tgttgcggta tgaggacatc aggcgcttcc tcgttacatc
     2281 tattgaaaat accgttcagg tcgaagacgt ccggttggtg gtgccacggg atgacgacga
     2341 aaggtcccgg gccgtctggg gcaacagggt gatagtcgat gtcctggaaa gacagaaggc
     2401 gcctatcagc aaggggtcga gtgttataag tcgccttgcc gacggagaga ggaaggcttt
     2461 cgagcgactg ttcgaggtat gtggcgccgt gattcttttt cccgtcatct tcaggggcag
     2521 gttgaacggg ctcatcaccc tcggcccgaa gaagtcggga gagctttttg tgcaggaaga
     2581 cctggagctt ctgagcactg tggccaatca gtgcgcggtg gccctggaaa acgccagggc
     2641 ctatgaagag ctccaggaat tgaacgcgaa cctcgagggg aaggtacgtg aaagaacggc
     2701 cgagctggag cgggccctgc gggaaaaaga gaatgcccaa aaccggctca tccagtcgga
     2761 gaggttggcg gcgatcgggc agctggcggc gggggtcgcc cacgaaatca acaaccccct
     2821 t
//
LOCUS       DAOTMX010000240         2811 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_27952_length_2811_cov_5.728592, whole genome shotgun sequence.
ACCESSION   DAOTMX010000240 DAOTMX010000000
VERSION     DAOTMX010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27952_length_2811_cov_5.728592"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="P5573_06980"
     CDS             complement(<1..340)
                     /locus_tag="P5573_06980"
                     /inference="COORDINATES: protein motif:HMM:NF018021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HRT27840.1"
                     /translation="MKLSEKEFDKVVRRAVNRIPKPIRRHLKNVLITVRKRPPRDMLE
                     ELGIPPDEPLPLGLYVGASLLERSETQPPLYPDSIFIFQEPLEEMCGTVEELERQIEI
                     TVVHEVAHFIG"
     gene            complement(435..731)
                     /locus_tag="P5573_06985"
     CDS             complement(435..731)
                     /locus_tag="P5573_06985"
                     /inference="COORDINATES: protein motif:HMM:NF015735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HRT27841.1"
                     /translation="MQFLVFGYDGKDDKAMERRMAKREAHLAGVAKMKAEGKALYGIA
                     MLDDEENMVGSVMVMDFPSRAELDGWLKVEPYVVGKVWKRVEVFKAKVPPIFLP"
     gene            1220..2164
                     /locus_tag="P5573_06990"
     CDS             1220..2164
                     /locus_tag="P5573_06990"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HRT27842.1"
                     /translation="MNEDDFLEALFKRLPSPPPDLVIPPGDDCAGLRVGRGRLLLVAI
                     DQLVGGRHYVLEGPGAAKPEEAGRKLLARNLSDIAAMGGRPLYCLTATASSPRRDARW
                     LRRFFDGLIALAGEFGVLMVGGDLAVGIADDVASLAVLGEVGEKQVVRRSGARPGDLL
                     FATGCFGRSVRTGHHLSFKPRCREGEWLARNGYARAMIDVSDGLLIDAHRLCRSSGVG
                     LRLETERIPRRTPDTTLREALTDGEDYELLFAVAGKKEGRLREMWPFADVPLTEVGVF
                     VACETPFVSDADGLSLPVEGYDHFSSFLGGIEGTTEGD"
     gene            complement(2113..>2811)
                     /locus_tag="P5573_06995"
     CDS             complement(2113..>2811)
                     /locus_tag="P5573_06995"
                     /inference="COORDINATES: protein motif:HMM:NF019142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HRT27843.1"
                     /translation="MHRAFIIAFCVFVAIAGASVSRAESVPDDATVAIHANEKAVPEK
                     KDDDRKQQVSEKMVTELRIVPFQKTQKPDSTMPRGTVKVVQPGREGRELVTYLIKMVG
                     GKEVERTAVGVEVLSKPRQHIELVGTAPVLSERTMTELRAIPFQKEQKPDDSLPAGTV
                     KVIEEGREGQERITYRIKLVDGKETERIVVNSEVLSPPVTRVEVVGTGKVVQEQPIQS
                     PSVVPSMPPRKEEK"
BASE COUNT          543 a          811 c          790 g          667 t
ORIGIN      
        1 tgccgatgaa atgggcgact tcgtgcacca ccgtgatctc tatctgccgc tccagttctt
       61 cgaccgttcc acacatctct tcgaggggtt cctgaaagat gaatatactg tcgggataaa
      121 gaggcggctg tgtctcggag cgctccagga gcgatgctcc cacataaaga ccgaggggca
      181 ggggttcatc cggcgggatg ccgagctctt ccagcatgtc cctgggaggg cgtttcctga
      241 cggtgatcag gacgtttttt agatgacggc ggatcggttt tgggatgcgg ttgacggccc
      301 gcctgacgac cttgtcgaat tccttctcgc tcagtttcaa ctctaatccc cggcagtctg
      361 agtccgcccc gagagggctc gtgacgggat aattatgttt cccctcaggt tgtagaaggc
      421 gactcgattt atgatcaggg taaaaagatc ggcggcactt tcgccttgaa cacctcgacc
      481 ctcttccata ccttgccgac tacatagggc tccaccttga gccagccgtc cagttctgca
      541 cgggaaggga aatccatgac catgacggag ccaaccatgt tttcctcgtc gtccagcatg
      601 gctatgccgt acagggcctt gccctcggcc ttcatcttcg ccactccggc gagatgcgcc
      661 tcccgcttgg ccatccgtct ctccatcgcc ttgtcatcct tgccgtcgta accaaaaacc
      721 aggaattgca tgataccctc cttgatcagt tgaccgaatg gatattcttg aattgccggc
      781 aaactcacca atgttatcag ccaagctttc cgacaaaaga acacataaaa aacgaaaaat
      841 caagcaggac gtccccgtaa aaagtccaaa aacgtcatgc cggacttgat taagcctgcc
      901 ccgtactcga tacgggggca tccagaacat attgaatata ctggattccg gcgttcgccg
      961 gaatgacgaa acgcgtaatt ctcgactttt tacgagtcca tcaagtaggt ctgggtcggt
     1021 atttcccgca ccaccgtgtt gatcagaaaa gaaacgatta ttgacttgga aatctctgcg
     1081 ccatctgtta tacattttca taacacattg aaatcattga atgcaagacg gaacatgggc
     1141 atgaaacctt gcgggagggc gaacctgccc gctgctgtgt ccgaaggttc tattgtccga
     1201 gaggaggaag aatttttcta tgaacgagga cgatttcctg gaagccctgt tcaagcgttt
     1261 accctcgccg ccgcctgacc tggtgatccc tcccggcgac gattgtgcgg ggttgcgggt
     1321 aggtcgcggc cgtcttcttc ttgtggctat cgaccagctc gtcggaggca ggcactacgt
     1381 gcttgagggg ccgggagccg ccaagccgga ggaggcggga aggaagcttc ttgcacgaaa
     1441 cttgagtgat atcgctgcga tgggtgggcg gccgctttat tgtttgacgg cgacggcgtc
     1501 gagcccccgt cgcgatgcac ggtggctgcg gcggtttttc gacggcttga tcgccttggc
     1561 cggagaattt ggcgtcctga tggtcggtgg cgacctggcg gttgggattg ccgatgacgt
     1621 ggcgagtctc gcggttctcg gcgaggtagg ggagaaacag gtcgtccgcc gttccggcgc
     1681 ccgtcccggt gaccttctgt tcgccaccgg ttgtttcggg cgctcggtgc ggacagggca
     1741 tcatctgagc tttaagccgc gctgccgtga aggggaatgg ctggcaagaa acggctacgc
     1801 ccgggcgatg atcgacgtaa gcgacggcct cctcatcgac gcccaccgtc tgtgcaggtc
     1861 ttcgggagta ggcctccggc ttgaaacgga gcgtatcccc cgtcgcaccc ctgacacgac
     1921 cctgcgggag gcccttacgg acggagagga ttatgaattg ctctttgcgg tcgcgggaaa
     1981 aaaggaggga cgtttgcggg aaatgtggcc ctttgcggat gtacccctca cggaagtagg
     2041 ggtgttcgta gcctgtgaga cccccttcgt ctcagacgcg gatggtttgt ccctccctgt
     2101 cgagggttat gatcacttct cctctttcct cggtggcata gaaggcacga cggagggtga
     2161 ttgaatcggt tgctcctgga cgaccttgcc ggttcccact acctcgaccc ttgtgacggg
     2221 cggagaaaga acctccgagt tcactacgat cctctccgtc tcctttccgt ccaccagttt
     2281 tatccggtaa gtgatgcgct cctgcccttc ccgtccttcc tcgatgacct tcaccgtccc
     2341 ggcaggcagg ctgtcgtcag gtttttgctc tttctggaac ggtatcgccc gcagctccgt
     2401 catagtgcgc tccgatagga cgggtgccgt cccgacaagc tcgatgtgct gcctcggttt
     2461 cgagaggact tcaacgccca ctgcggtccg ttcgacttcc ttcccgccca ccatctttat
     2521 cagataggtc accaactcac gaccctcccg acccggctgc acgaccttga ctgtacctct
     2581 gggcatggtg ctgtccggtt tctgtgtttt ctgaaagggt acgatacgca gttccgtgac
     2641 catcttctcc gaaacttgct gttttctgtc gtcgtctttt ttctccggca ccgccttttc
     2701 gtttgcgtgg atggcgacag ttgcgtcatc cggcaccgac tccgcccgcg atacggaggc
     2761 gccggcgatt gcaacgaaca cacaaaaagc gatgatgaaa gccctatgca t
//
LOCUS       DAOTMX010000241         2809 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_27978_length_2809_cov_3.448439, whole genome shotgun sequence.
ACCESSION   DAOTMX010000241 DAOTMX010000000
VERSION     DAOTMX010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27978_length_2809_cov_3.448439"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="P5573_07000"
     CDS             <1..363
                     /locus_tag="P5573_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27844.1"
                     /translation="IDVVTIHYEIGSADAASGSHRMYELATSAMKVFYRLRERNGDNQ
                     KVPQSVLKRKETDLYRWVGHASFQKGRYADARRYLTKRVLRIPFDYQSLALLMISCMP
                     FPSAVIRMIKSVGNRGAA"
     gene            428..1510
                     /locus_tag="P5573_07005"
     CDS             428..1510
                     /locus_tag="P5573_07005"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRT27845.1"
                     /translation="MKTEIVTSIKEFEAIRGDWNKILEGSISNSIFLTWEWMYSWAEC
                     FINERRELFVVKVYDDRNRLVGIAPWYIEKIKIGPAAIRQVSFLGTPETASDYLDVFT
                     ARGREREVSSYLYDALFGELAGSWDCISLQDTRAESLFLLSFMYKHCLQGRHFEITQG
                     SFCPIISLPKTEEEFLSQISSSRSKRFRQDLRTLKKTDTVEIKTHVADGIEEAVDGFF
                     KLYAEKTEWEGKDLHRFIKRFVGNAENGKYLQIDFLLANGNTIGGLLHLKHNHALSLY
                     LMAVDKAYNPRISTGNLLVGLCIVRAIENQFKVYDFLKGYEDYKFYWTSEGRTTQTVL
                     IVQKKTLPVIHMMKRMAKNALKTMLR"
     gene            1514..2485
                     /locus_tag="P5573_07010"
     CDS             1514..2485
                     /locus_tag="P5573_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRT27846.1"
                     /translation="MTKSIMHLVVTIDTEEDNWGQYSEDSFSIENIKKIPALQKIFDE
                     FSVVPTYLVTYPVATDRESIKILKDILQQGKCEIGMHCHPWSTPPFDDERNSFNSMLS
                     NLPFDLQCKKMGVLHEAIERNFGVTAKSFRAGRWGYNGHTALVLKKLGYRVDTSITPF
                     TDWSEYHGPDFSEMKPESYRFNAPEIFNSIPEGEMAEIPATIGYLQNYYELCNSTDRL
                     LSSPLGRLFRMKGLLNRAGLLNKVLLSPEGSDSGDMVRLTKVLMRKKFEIVNLFFHST
                     SLQAGLSIFVKTKEDEGRFSDRIRGYLAFTSEAGISPITLSDAADVV"
     gene            2584..>2809
                     /locus_tag="P5573_07015"
     CDS             2584..>2809
                     /locus_tag="P5573_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27847.1"
                     /translation="MNRGLKVGKNVYIFQDVEFDSGYPFLIEIGDNCRIGKNVLILAH
                     DATTFKELGITRLAPVKILEGSFIGQRAVIL"
BASE COUNT          792 a          606 c          739 g          672 t
ORIGIN      
        1 atcgacgtgg tgaccattca ctatgaaatt ggctccgcag acgcggcgag cggaagtcac
       61 cgcatgtacg agcttgcaac aagtgccatg aaggtgtttt acaggctgag ggaacgcaac
      121 ggagacaatc agaaagtccc gcagagtgta ctgaaacgta aggaaaccga cttatacagg
      181 tgggtggggc atgcgtcctt tcaaaagggg cgatatgcgg atgcaaggcg atacctgacg
      241 aaacgggtac tccgcattcc ctttgattat caatctcttg ccttactcat gataagttgc
      301 atgcctttcc catccgccgt gatccggatg ataaagtcag tgggcaatcg cggggccgca
      361 tgacgacatc aaaataaatg gctctgccgc gcgataattg ctcaagagtg atcaaggggt
      421 ggattgattg aagacggaga ttgttacatc aataaaagaa tttgaggcga tccggggtga
      481 ttggaacaag atactcgagg gatccatatc caattcgata tttctgacgt gggaatggat
      541 gtattcgtgg gcggagtgtt ttatcaatga aagacgcgaa ctctttgtag tcaaagtgta
      601 tgacgaccgc aacaggctgg tagggatcgc gccctggtat atcgaaaaga taaaaatagg
      661 gccggcagca atcaggcagg tcagtttttt aggaacgccg gagacggcgt ccgattacct
      721 cgatgtattc acagcaaggg gaagagaaag agaagtcagc agttatttgt acgatgctct
      781 tttcggagag ctggcaggca gctgggattg catcagcctg caggacacaa gagctgaatc
      841 cttgtttctt ttgagtttca tgtacaaaca ttgtctacag ggaagacatt tcgagatcac
      901 gcaggggtcg ttctgcccca tcatatcttt gccgaaaacc gaggaagaat ttttatctca
      961 gatatcgtcg agcaggagca agcgcttcag gcaggatctg agaacattga agaagacgga
     1021 caccgttgag ataaaaacgc acgtggcaga cggtattgaa gaagcggtgg acggtttttt
     1081 caagttatac gccgagaaaa cagagtggga agggaaagac cttcacaggt tcataaagcg
     1141 cttcgtggga aacgcggaaa acggaaaata cctccagatc gattttctcc tcgcaaatgg
     1201 gaataccata ggcgggctcc tgcatctgaa gcacaaccat gcgctgtcac tgtacctgat
     1261 ggccgtagac aaagcatata acccgagaat aagcacgggc aacctgcttg tcggcctctg
     1321 catcgtaagg gcgatcgaaa atcagttcaa ggtttacgat tttctcaaag ggtacgagga
     1381 ttacaaattt tactggacga gcgagggtcg cacgacccag accgtgttga tcgttcaaaa
     1441 gaagactctg ccggtcatcc atatgatgaa gagaatggca aaaaacgccc tgaagacgat
     1501 gctgcggtag gaattgacaa aaagcattat gcaccttgtg gtcaccatag atacagagga
     1561 agataactgg gggcaatact cggaagactc tttcagtatt gaaaacatta aaaaaattcc
     1621 ggcgcttcaa aagatattcg atgaattcag tgtcgtgccc acctatcttg tcacttaccc
     1681 tgtcgccact gacagggagt cgatcaaaat cctgaaagac attcttcaac agggcaaatg
     1741 cgaaatcggg atgcactgcc acccctggag cacgccgcct tttgatgatg aacggaattc
     1801 ctttaacagc atgctctcca acctgccttt tgaccttcag tgcaagaaaa tgggtgtgct
     1861 tcatgaagcg atagaaagaa attttggtgt cacggcaaaa tccttcaggg ccggcaggtg
     1921 gggttataac gggcacacgg cgcttgttct taaaaaactg ggctatcggg tggatacctc
     1981 aataacgccc tttacggact ggtcggagta tcacggcccg gatttttctg aaatgaaacc
     2041 ggaatcatac cggttcaatg caccggaaat ttttaatagc atccctgagg gtgaaatggc
     2101 ggaaataccc gccaccatcg ggtatcttca gaattactat gaattgtgca attcgacgga
     2161 tcgtctcctg tcatctccac tgggcagact gtttcgcatg aagggattgc tgaacagggc
     2221 aggattactg aacaaagtgt tgctgtcgcc cgagggttca gactcgggcg acatggtcag
     2281 attgacgaaa gtcttaatga gaaaaaaatt tgaaattgta aatctgttct tccattccac
     2341 gtcattgcag gctggtttga gtatttttgt gaaaacaaaa gaagacgaag gccggttttc
     2401 ggatcgtata aggggatatc ttgcgttcac aagtgaggca gggatcagtc cgatcactct
     2461 ctccgacgcc gcagacgttg tttagcgagc ggggaattca tgattctgaa tttgttgaaa
     2521 aagattaatc ggatagtcac gtatatttcc tttaaacggg atcgtcttcg attaaaaagc
     2581 ctcgtgaaca gggggctcaa ggtcgggaag aatgtctaca tattccagga tgtggagttt
     2641 gattccgggt acccctttct catagaaata ggagataact gccggatcgg aaaaaacgtt
     2701 ctcattctcg cccatgatgc caccactttt aaggaactcg gcatcactcg tctcgctccg
     2761 gtgaagatcc tggagggttc gttcatcgga caacgtgcgg tcattttgc
//
LOCUS       DAOTMX010000242         2799 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_28084_length_2799_cov_4.575802, whole genome shotgun sequence.
ACCESSION   DAOTMX010000242 DAOTMX010000000
VERSION     DAOTMX010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28084_length_2799_cov_4.575802"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="P5573_07020"
     CDS             <1..245
                     /locus_tag="P5573_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27848.1"
                     /translation="TKKLIVLLMVAAFTFGMVGLGFSAQEVKGTVSKIEGIQLTIQDE
                     MGKQTTVKVEDPADLKDLQIGDKVLVKDGKVTKEGK"
     gene            637..2169
                     /locus_tag="P5573_07025"
     CDS             637..2169
                     /locus_tag="P5573_07025"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HRT27849.1"
                     /translation="MLRNGKKLTASLLLLFLVVFPLTFLSAEAAGAPVGITILHVNDT
                     HGRILPEVEKGVNKKRPVGGAAWLAKMVEKEREKNPGGTLLLSAGDMFQGTAVSREFN
                     GKPVLDVMNAMKFDAMAVGNHEFDWGKNVLADLSRAARFPFLSANIRDGDGKRPPWLK
                     PYVILERKRLKIAVIGMTTELTRYIVKPENVKGLSFIEVGDALPPAIKEAKSQGAKTI
                     VVLSHAGLDADKQIAQEIPGIDVIVGGHSHTAVTTPVVAGRTIIVQAGCCGHFLGVLR
                     LKIDPESGRVVEFMKTRVLKKVYSGPNDPFDPNIAGIVKRYHDRIKDRFSVVVGETAV
                     DLVRNYGGESNIGNLIGDAMREAEKADIAFHNSGGIRGNVPAGKITLEQLYAVLPFDD
                     VLVSMDLTGKQILLILEQNAAVERGILQVSGLKVKYDMTRPVGERVVDVEVGGRPLRP
                     GKSYRVVTTDFVAAGGEKFAAFREGKNVRYGDDMRDALVAYLKRHSPVRPEIEGRIVF
                     VK"
     gene            2169..2396
                     /locus_tag="P5573_07030"
     CDS             2169..2396
                     /locus_tag="P5573_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27850.1"
                     /translation="MEERLTVFVNGKPVTVFRGMTVKHALISLDYALYRDVRDGKAVV
                     KDENGFTVGLGGALHDGARFYLDMAGRTGCR"
     gene            complement(2406..>2799)
                     /locus_tag="P5573_07035"
     CDS             complement(2406..>2799)
                     /locus_tag="P5573_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HRT27851.1"
                     /translation="GAVIIAGSGMCEGGRIRHHLKHNIWRSECSIIFVGFQAEGTLGR
                     QIVEGAQRVHFLGEDFAVRARVYTIGGFSAHGDQRELLEWLGLFKAKPEIFIVHGEEK
                     SALEFERAVKKKLGLVTHVPYLGNSFDI"
BASE COUNT          675 a          722 c          826 g          576 t
ORIGIN      
        1 agaccaagaa gttgattgtg ttgttgatgg ttgcggcatt tacttttggt atggttggcc
       61 tgggcttttc ggcgcaggag gtaaaaggca cagtttctaa gatagaaggg atccaactga
      121 ccattcagga cgaaatgggt aaacagacaa cagtaaaggt cgaagatccg gctgacttga
      181 aggatctaca gatcggtgac aaggtattag taaaggacgg caaggtaacg aaagagggta
      241 agtgatcatt atatcgttct taaagtgaca cctcgataga aaatcgaaag cgtataatac
      301 tgcccctgga tgtatcaccc cgatgagatt ctgggtgata catccggtgt tttcaatgtg
      361 tgctggattt ctgtcctcct cctcttccct gctgcacaca tacctaccat catggcctgc
      421 gtgtctcgcc ggttcggcag cttctgtcag cggatagaaa aaacaagtaa caacatgccg
      481 tcaccttttt gtgaagatgg acctgctggg ggcagagatt aaggcccgag cccgatcaac
      541 aagaagaagg gttccagaaa agggctgtcg ggttgtgact tgaaatcgac cgtaacttag
      601 gtaaaatggc cgacagctcg ctcgaaagga acgaatatgc tccgcaacgg aaagaaactt
      661 acagcatccc tcctgcttct attcctggtc gtctttcccc ttacgttcct ctctgccgag
      721 gcggcgggag cgccggtcgg aatcacgatc ctccatgtca acgacaccca tggacgaatc
      781 ctgccggagg tcgagaaagg tgtgaacaag aaaaggccgg tggggggcgc agcctggctg
      841 gccaaaatgg tcgagaagga aagagagaaa aacccggggg ggacgttgct gctctcggcg
      901 ggggatatgt tccaggggac ggcggtatcg agggagttca acgggaagcc ggttctcgat
      961 gtcatgaacg ccatgaagtt cgacgccatg gccgtcggga accatgagtt cgactgggga
     1021 aagaatgtcc ttgccgacct ctcccgagcc gcccgttttc ctttcctttc ggccaacata
     1081 agggatggtg acggaaagcg cccaccgtgg ctaaagccct atgtaatact ggagcgcaag
     1141 cggctgaaga tagccgtcat cgggatgacg acggaattaa ccagatacat tgtaaaaccg
     1201 gaaaatgtga aggggttgag ctttattgag gtgggcgacg ccctgccacc ggccatcaag
     1261 gaagcaaaat cgcagggggc gaaaacgatc gttgtcctct cccatgcggg cctcgacgcc
     1321 gacaaacaaa tagcgcagga aattcccggc atcgacgtca ttgtcggcgg ccacagtcat
     1381 actgcggtca ctacacccgt cgtcgccgga agaacgataa tagtgcaagc tggatgctgc
     1441 ggacattttc tcggcgtcct aagactgaag atagaccccg aaagcgggag agtcgtagag
     1501 ttcatgaaga caagggtgct gaagaaggtc tattccggcc ccaacgaccc tttcgacccc
     1561 aatatcgccg ggatcgtgaa gcgttaccat gaccggatca aagaccgttt ctctgttgtc
     1621 gtgggtgaaa cggcggtaga cctggtcagg aattacggag gagaatcaaa catcggcaac
     1681 cttatcggcg acgccatgag ggaggcggag aaggctgata tcgcctttca caacagcggt
     1741 ggcatccgcg gaaatgtgcc ggcgggcaag ataaccttgg agcaattata cgccgtgctt
     1801 cccttcgacg atgtcctcgt gagcatggac cttacgggta agcagattct cctaatcctc
     1861 gagcagaacg ccgcggtgga gcgcggcatc ctccaggttt ccggactgaa ggtaaaatac
     1921 gacatgacga ggcccgtcgg cgagcgggtc gtggacgtgg aagtgggggg gaggcccttg
     1981 cgcccgggta aatcctacag ggtcgtgacg accgatttcg tggccgccgg gggggagaaa
     2041 ttcgcggcct tcagggaagg taagaacgtg cgctacggcg acgacatgcg tgacgcctta
     2101 gtcgcttact taaaaaggca ttcgccagtc cgccctgaaa tagagggccg gatagtgttc
     2161 gtgaagtgat ggaggagcgg ctgaccgttt tcgtgaacgg taaacccgtc accgttttcc
     2221 ggggcatgac agtgaagcac gccctgatct ccctcgacta tgccctttat agggacgtca
     2281 gggacggaaa ggcggtcgtt aaggatgaaa acggcttcac cgtcggcctc gggggcgccc
     2341 ttcatgacgg agcgaggttc tatctcgaca tggccggaag gacgggctgt cgctgacggc
     2401 aaaaatcaga tgtcgaatga gtttccaaga taaggcacgt gagtcacgag gcccagtttt
     2461 ttcttcacgg ccctttcgaa ctcgagggcg ctcttttctt cgccgtggac gataaagatc
     2521 tccggcttgg ccttgaagag gccgagccat tccaggagtt ccctctggtc tccatgggcg
     2581 gaaaagccgc cgatcgtata gaccctggcc ctcacggcaa aatcctcacc cagaaagtgc
     2641 accctctgcg ccccctcgac gatctgcctc ccgagggtgc cttccgcctg aaagccgacg
     2701 aaaatgatac tgcactccga gcgccagata ttgtgcttca ggtggtgcct gatgcgtcct
     2761 ccctcgcaca taccgctacc ggcgatgatt actgccccc
//
LOCUS       DAOTMX010000243         2773 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_28429_length_2773_cov_3.587196, whole genome shotgun sequence.
ACCESSION   DAOTMX010000243 DAOTMX010000000
VERSION     DAOTMX010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28429_length_2773_cov_3.587196"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1515
                     /gene="groL"
                     /locus_tag="P5573_07040"
     CDS             <1..1515
                     /gene="groL"
                     /locus_tag="P5573_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417396.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HRT27852.1"
                     /translation="KTFGAPTVTKDGVTVAKEIELEDKFENMGAQMVREVASRTSDVA
                     GDGTTTATILAQAIYREGAKNVAAGYNPMDIKRGIDKAVEIVVEELGKLSKPTKDQKE
                     IAQVGTISANNDETIGNIIAEAMNKVGKEGVITVEEAKGLETELDIVEGMQFDRGYLS
                     PYFVTNPEKMEVNYDDPYILIHDKKISNMKELLPILEQIAKMGKPLLIIAEDIEGEAL
                     ATLVVNKLRGTLQVVAVKAPGFGDRRKAMLEDIAILTGGKLISEEMGAKLENTTIADL
                     GRCRKLTVDKDNTTIIDGAGDRASLEGRVKQIRAQIEETTSDYDREKLQERLAKLVGG
                     VAVIKVGAATETEMKEKKARVEDALNATRAAVEEGIVPGGGVAYIRCIPALEKLALDG
                     DQQVGVAIVKKALEEPLKMIAQNAGAEGNIVVEKVKEKKGSFGFNAATDEYEDLVKSG
                     VIDPKKVTRFALQNASSVASLMLTTQCMVAEKPKKETMPPMPPGGMGGYGGMDM"
     gene            1689..>2773
                     /locus_tag="P5573_07045"
     CDS             1689..>2773
                     /locus_tag="P5573_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRT27853.1"
                     /translation="MDYKDTLNLPKTDFPMKANLAKKEPEMLQAWEAMNIYGKIREVS
                     KGRPVYILHDGPPYANGDIHLGTALNKIIKDIVIKSKNMSGFDSIFVPGWDCHGLPIE
                     HQVDKELGDRKNDLPRVEKRRYCRKYAEKYVDIQRSQFKRLGVFGEWENPYLTMSYEY
                     EAVTVAEFAKLMLSGDVYKGKKPVYWCAHCKTALAEAEVEYADHDTPSVYIKFPALSD
                     FGDVLPRLKGEKVYVLIWTTTPWTIPANLAIALHKDFVYAAVKVQGEVLILAKELVDY
                     CMDAFGFKQYEIIAEFPGAVLEGLKTNHPFLDRESVLILAPFVTLDAGTGAVHIAPGH
                     GQEDYEIGLEYGLDNYAPVDEDGRFTK"
BASE COUNT          725 a          712 c          842 g          494 t
ORIGIN      
        1 aagaccttcg gtgcaccgac ggtgacgaag gacggtgtga cggtggccaa ggaaatagag
       61 ttggaagaca agttcgagaa catgggcgct cagatggtca gggaagttgc gagccgcacg
      121 agcgatgtgg ctggagacgg caccacgacg gcgacaatcc tggcccaggc tatatacaga
      181 gaaggcgcaa agaacgtagc ggcggggtac aaccccatgg atatcaagag aggcatagac
      241 aaggcagttg agatcgtggt cgaagagctc ggcaagttaa gcaagccgac gaaggaccag
      301 aaggagattg ctcaggtcgg tacgatttcc gccaacaacg acgagacgat cggcaacatc
      361 atcgccgagg ccatgaacaa ggtgggcaaa gaaggtgtca tcacggtgga agaagccaag
      421 gggctggaga cggaattgga catcgtggaa ggaatgcagt tcgaccgggg ttatctctcc
      481 ccttatttcg tcaccaatcc cgagaagatg gaagtcaact atgatgaccc ttacatcctg
      541 atccatgaca agaagatcag caacatgaaa gagcttctcc ccatcctcga gcagatagcc
      601 aagatgggaa aacccctgct gataatcgcc gaagacatcg agggtgaggc cctggccacg
      661 ctcgttgtga ataaacttcg ggggacgttg caggtggttg ctgtaaaggc tcccggtttc
      721 ggcgacaggc ggaaagcgat gcttgaagac atcgcaatcc tcacgggtgg aaagctcata
      781 tcggaagaga tgggagcgaa gcttgaaaac acgacgatcg ccgaccttgg tcgttgccga
      841 aaacttaccg tcgacaagga caacacgacg ataatcgacg gtgccggtga cagggcaagc
      901 ctcgaggggc gcgtcaagca gatccgggcg cagatcgagg agacgacgtc cgattacgac
      961 cgcgagaagc tccaagagag gttagcaaag ctcgtcggcg gtgtggccgt gatcaaggtg
     1021 ggtgcggcta cggagacgga aatgaaggaa aagaaggccc gggtggaaga cgcactgaac
     1081 gcaacacggg cggccgtgga ggaaggaatc gtccccggcg gtggcgtcgc ctatatcagg
     1141 tgcatccctg cgctcgaaaa actggccctg gatggtgatc agcaggtcgg cgtcgccatc
     1201 gttaagaagg ccctcgagga acctctcaag atgatcgctc agaacgcagg agcggaaggc
     1261 aacatagtcg ttgagaaggt aaaggagaag aaggggtcct ttgggttcaa cgcggcgacg
     1321 gatgaatatg aggacctcgt gaaatccgga gtgatcgacc ccaagaaggt gacgcgcttt
     1381 gcactccaga atgcgtcgag cgttgcgtca ttgatgctca ccacccagtg catggtggcc
     1441 gagaaaccga agaaggaaac catgccgccg atgcctccag gagggatggg cggttacggc
     1501 ggaatggata tgtaaaattc ccttaccggc agtctatgaa agaggccccg ggcttttccg
     1561 gcggggcctc tttttttgct ctctcccctt gaaagtcccc cttatctgtg gtattcccgt
     1621 caaaatcaaa aaaatcaacg cactatgagc ttttgcctca agctccgagc tcccgaaagg
     1681 aaatctccat ggactataag gatacgttaa acctgccgaa gaccgatttc cccatgaagg
     1741 ccaacctggc caagaaagaa cccgagatgc tgcaagcctg ggaggcgatg aacatttacg
     1801 ggaagatacg cgaggtatcc aagggacggc cggtctacat cctgcacgac ggtccgccgt
     1861 acgcaaacgg agacatacat cttgggaccg cactcaacaa gatcatcaag gacatcgtga
     1921 tcaagtcaaa gaacatgtcg gggtttgaca gcatcttcgt ccccggatgg gactgtcacg
     1981 gccttcccat agagcatcag gtggacaagg aattgggcga caggaagaac gacctgcctc
     2041 gggtggagaa aaggcgttac tgcaggaagt acgccgaaaa gtacgtcgac atccagcgca
     2101 gtcagttcaa gcgcctcggt gtcttcggcg aatgggaaaa tccctatctc acgatgagtt
     2161 atgagtacga agccgtcacg gtagcggagt ttgcgaaact gatgttgagc ggcgatgtct
     2221 ataagggaaa gaagcctgtc tattggtgcg cgcactgcaa gacggctctc gctgaagcgg
     2281 aggtcgagta cgccgaccac gatacgcctt ccgtctacat caagttccca gccctatcgg
     2341 acttcggcga tgtcctgccg agactgaagg gagaaaaggt atacgtgctc atctggacga
     2401 cgacgccatg gacgatcccg gccaacctgg cgattgccct ccataaggat tttgtttacg
     2461 ccgccgtcaa ggtacagggg gaggtcctga tccttgccaa ggagctcgtc gactattgta
     2521 tggacgcctt tgggttcaag cagtacgaga tcatcgcaga gtttcccggc gcggtgctcg
     2581 aaggactcaa gaccaaccac cccttcctgg accgtgagag cgtcctgatc cttgcaccct
     2641 tcgtcaccct ggacgcgggg accggcgccg tccacatagc gccgggacac ggccaggaag
     2701 attacgagat cggccttgaa tacggcctcg acaattacgc tcccgtggac gaggacggca
     2761 ggttcaccaa gga
//
LOCUS       DAOTMX010000244         2772 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_28442_length_2772_cov_3.152374, whole genome shotgun sequence.
ACCESSION   DAOTMX010000244 DAOTMX010000000
VERSION     DAOTMX010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28442_length_2772_cov_3.152374"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1093)
                     /locus_tag="P5573_07050"
     CDS             complement(<1..1093)
                     /locus_tag="P5573_07050"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HRT27854.1"
                     /translation="MKPRRHILLVNPWIHDFAAYDFWVKPLGLLYLAGLLRENGYYVR
                     LIDCLDSRHPALKSYPEVRTPKEKPFGQGKFARQVIPRPNPLSDIPRSYHRYGIPPAL
                     FMEELRRGEKPDAVLVTSMMTYWYPGVVDAIRLISQSFPGTPVILGGNYVNFCPTHAA
                     THSGADFVVPGAGERGIPDLFKSLFGDDVWFQPDPDDLDSYPYPAFDLLPQVNQVPFL
                     TSRGCPFRCTYCASHRLNPVFRRRNPERVAEEIAFWNRTLRVRDFSFYDDAFLFNPEE
                     MAVPLMREIRRMGLDCRFHCPNGLHLRDLTEDVAVLLRKTGFTTLRFGFETSNPDSQV
                     ATGGKTTNQDLSLAVRSLRHAGFSPKDIGV"
     gene            complement(1090..2469)
                     /locus_tag="P5573_07055"
     CDS             complement(1090..2469)
                     /locus_tag="P5573_07055"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HRT27855.1"
                     /translation="MQTFRKFSVFLVTVVILLQPVAAGAAFTVDDEVKLGKEFYDKLA
                     KLDILVQNERVNEYINRLGNAVLSQSSNVLFPFRFSVIKSSAVNAFATPGGYIYVNRG
                     LINLVENESQLAGVLAHEIAHVNSRHIAQQIEKSQKLNFATLAGILAGAFLGGGTGTE
                     ATLGLTLAATTSLSLKYSRENEEEADRLGISYLVNAGYDGSSMMSFLKLMKTYEFYSN
                     TLPSYFLTHPGTDERISYLDALVRVRYGKGGAKNILGGLGRVQALLMLEDRDTEAAIR
                     YFNNKLSKNPQDVDSLFGLAVAHMKLGQPAAAFANFSEALRISPDDPDILLELGKAYL
                     ASGRTSDAITLLRRSYELDDKDKETLLYLGRAYEEAGSYSTALEFYRQYSSKNPKDVN
                     IFYNMAMAYGKTGNNARSHYYFALYFKNKNKVESALFHLKEAQKYGDPELKAEIEKEI
                     ELLRPPKKK"
BASE COUNT          598 a          687 c          828 g          659 t
ORIGIN      
        1 agaccccgat gtctttgggg ctgaacccgg cgtgcctcag agatcggacc gccagcgaaa
       61 ggtcttgatt cgtcgtttta ccgcccgtgg cgacctgcga gtcggggttg gatgtctcga
      121 acccgaacct gagggttgtg aagccggtct tgcgaagaag aacggcgaca tcttccgtga
      181 ggtcgcgaag gtggaggccg ttcgggcaat ggaagcggca atccagtccc atcctccgga
      241 tttccctcat gagggggact gccatttctt cgggattgaa gagaaaggcg tcgtcataga
      301 aggaaaaatc cctgacccga agggtcctgt tccagaaggc gatttcttcg gcaacgcgtt
      361 cggggttccg cctcctgaat accgggttca gcctatggga ggcgcagtag gtacaccgga
      421 aagggcatcc tctcgaagtg aggaacggaa cctggttgac ctgcgggagg agatcgaagg
      481 cgggataggg ataggaatcg agatcgtcag ggtccggctg aaaccaaaca tcatcaccga
      541 ataaagactt gaaaaggtcg gggattcctc tttcgccggc accggggacg acaaaatcag
      601 cccctgagtg cgtcgcggca tgggtaggac agaaatttac gtagtttccg ccaagaatga
      661 ccggcgttcc cgggaagctc tgagaaatca gcctgattgc atcgaccacg ccgggatacc
      721 agtaggtcat catgcttgtt acaaggacgg cgtccggttt ttctcctctc cggagttctt
      781 ccataaagag cgcaggcggg atcccgtacc ggtggtaaga ccgtgggatg tcgctcaaag
      841 gattcggcct ggggatgact tgtcgggcga acttcccctg gccgaagggt ttttccttcg
      901 gcgtccggac ttccgggtaa ctcttcaagg cgggatgccg ggagtcaagg caatcgataa
      961 ggcgtacgta atagccgttc tctctcaaca ggcccgccaa gtatagcaat ccgagtggtt
     1021 ttacccaaaa gtcataggcg gcgaagtcgt gaatccaggg attgaccagc aggatgtgtc
     1081 tacggggctt catttttttt tgggcgggcg cagtagctcg atctctttct cgatttcggc
     1141 cttcaattcg gggtctccgt acttctgggc ctctttgaga tggaagaggg cgctttcgac
     1201 cttgttttta ttcttgaagt agagggcgaa gtaataatgc gaccgcgcgt tgttgccggt
     1261 tttgccgtag gccatcgcca tattgtagaa tatgttgaca tccttcgggt ttttggaaga
     1321 atactgccgg tagaattcga gggccgtgct gtagcttccc gcctcctcat aggccctgcc
     1381 gaggtaaaga agagtttctt tgtccttgtc gtcgagttcg taggaccgcc tgagcagggt
     1441 tatggcgtcg gacgtcctgc cgcttgcgag gtaggccttc cccagttcga gcaggatgtc
     1501 ggggtcgtcg ggagaaattc tgagggcctc cgagaaattc gcaaatgcag ctgcgggttg
     1561 accgagcttc atatgggcga ccgcaaggcc gaacaaggaa tcgacgtcct gtgggttttt
     1621 actgagcttg ttgttgaaat accttatggc ggcttcggta tccctgtcct cgagcatcag
     1681 aagggcctgg acgcggccta aaccgccgag gatgttcttt gcgcccccct ttccgtagcg
     1741 gacgcgaacc agcgcatcca gataagagat cctttcatcg gtgccgggat gggtcaaaaa
     1801 gtaggaagga agtgtattcg aatagaattc ataagttttc atcaacttca ggaagctcat
     1861 catgctgctg ccgtcgtatc cggcgttcac gagataagag atgccgagcc ggtcggcctc
     1921 ttcttcgttt tccctgctgt acttgaggct cagagaagtc gtagcggcca aggtcaatcc
     1981 cagggtcgcc tccgtgcccg taccaccgcc gagaaaggcg ccggccagga tgccggcgag
     2041 ggtggcgaag ttgagcttct gggatttttc gatctgctgg gcgatgtgtc tcgaattaac
     2101 gtgggcgatt tcatgggcca gtacgcccgc gagttgactt tcgttttcca cgaggtttat
     2161 caaccccctg ttgacgtaga tgtagccgcc gggcgtagca aaggcgttga ccgcggagct
     2221 tttgatgacg gagaaccgaa agggaaacag cacattgctg ctctgtgaga gaacggcgtt
     2281 cccgaggcgg ttgatgtact cgtttaccct ctcgttctgg acaagaatgt cgagctttgc
     2341 gagtttgtcg tagaattcct taccgagctt gacttcatca tcgaccgtga aggccgcacc
     2401 ggctgctacc ggctggagca agatgacgac ggttaccaaa aataccgaaa atttcctaaa
     2461 agtctgcata aaactcgact cgtatgatca accctgatga accgatcttg atatttaatg
     2521 gggttcatgc ccaaccactt ttttgccgaa tcatgatgca ttgtacagca actctcttct
     2581 gtaatcaagg caacttccct aaacattcag agggattgtg gggggctttg ctttgtggta
     2641 tacgcttcac gtcttccgtc tgccgggaag ggggaaagct caggagattg aaaaagtctt
     2701 tgacgataac ggtagatggg ccggcggggg cgggtaagag tacggtcagt aagcgtcttg
     2761 cggaccgact tt
//
LOCUS       DAOTMX010000245         2754 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_28698_length_2754_cov_4.002223, whole genome shotgun sequence.
ACCESSION   DAOTMX010000245 DAOTMX010000000
VERSION     DAOTMX010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28698_length_2754_cov_4.002223"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..428
                     /locus_tag="P5573_07060"
     CDS             60..428
                     /locus_tag="P5573_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27856.1"
                     /translation="MKKVMVVMIATLFVFGISFYFIADLARAENPAVWAAKKAAGKID
                     KKIESKKQAKKEAEEKKKKEEADAIAKKEADEAFAEKQKRDAEDIAAWREEKKAKGEP
                     QHMSKEEIKRRKINRQPIVY"
     gene            complement(477..677)
                     /locus_tag="P5573_07065"
     CDS             complement(477..677)
                     /locus_tag="P5573_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27857.1"
                     /translation="MTRYKRLLRLEEEKNGQDVDLTEEQIRFLEKANPCFRERRVEHR
                     RTKVARPRTNGFVERFNRTVLD"
     gene            complement(1239..2033)
                     /gene="trpA"
                     /locus_tag="P5573_07070"
     CDS             complement(1239..2033)
                     /gene="trpA"
                     /locus_tag="P5573_07070"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HRT27858.1"
                     /translation="MKRIEKAFRSLRAKNEKALIAYVTAGDPSLEKTEEVLLALDKAG
                     VDILEVGVPFSDPTADGPVIQAASQRALKNGVTLDAILDMVAALRRASEIPIVLFGYY
                     NPIFAYGNERFALKAKEAGVDGILVVDLPPEESGELRRYTDREKIDFISLVAPTTGND
                     RVEEIAKKASGFIYYISVTGVTGTARPEIEDISRDVARIRKRSDLPVAVGFGISTPEQ
                     ARRIAPLADGVVVGSAFVKLIEENSGRRDLADVVFSFARGLKAALP"
     gene            complement(2030..>2754)
                     /locus_tag="P5573_07075"
     CDS             complement(2030..>2754)
                     /locus_tag="P5573_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRT27859.1"
                     /translation="VVPVHSGTATLKDAMNEAMRQWVAHVRDTFYVIGTTAGPHPYPM
                     MVRNFQSVIGRETKRQILRQEGRLPDYLIACVGGGSNAMGLFYPFRKDTAVSLVGVEA
                     AGKGIATGLHAASISAGRVGVLHGNKTYLLQDDSGQVRDAYSIAAGLDYPGVGPEHGY
                     FHATGRARYVNVTDDEAVQAFMELSHLEGIIPAMESAHAVAYAMKLAPGLDRDRIIVI
                     NLSGRGDKDAAVIAESMEGRQT"
BASE COUNT          570 a          777 c          812 g          595 t
ORIGIN      
        1 aaacccctgt ccgactgccc taaccaaaac tgtctaactc caggggtgca gtccagaaga
       61 tgaaaaaagt tatggtagtt atgatcgcaa ctctttttgt gtttggcatt tcgttttatt
      121 ttatagccga cctggcgcgg gccgagaacc cagctgtgtg ggcggcaaaa aaggctgcgg
      181 gaaagataga taagaaaatc gaatccaaga aacaggctaa aaaagaagct gaagagaaga
      241 aaaagaaaga ggaagccgat gcgatagcca agaaagaggc agatgaagcc tttgcggaaa
      301 aacagaaacg ggatgccgaa gatatagccg cgtggaggga agagaagaaa gccaaaggcg
      361 aaccccaaca tatgagcaaa gaggaaataa agaggcgcaa aataaacagg caaccgattg
      421 tttactgata cgtaaaactc acgttgcggt agccccggtg cggcctctcg taggaattaa
      481 tcgagaacgg tacggttgaa tcgctccaca aagccgttgg tcctgggtct ggccaccttg
      541 gtgcggcgat gctcgactct gcgctcccga aagcagggat tggctttctc cagaaagcgg
      601 atctgctcct cggtgaggtc gacatcctgg ccgttcttct cttcttcgag ccgcaggagc
      661 cgtttatagc gtgtcacgag gtcttctttt atccagatgt tgcggaccgt gctggggctt
      721 acgaagatgc cctctaagcg aagattatcg gatacgcgga cccggttcga acccaggctg
      781 gatgcttcag agaaagggcg attatcttct ctcttacatc ggaaggcgtc tcattgggaa
      841 cgctcttggg gatcggaggc ctgtccatga ggccgtcaaa gcccctttcc tgaaaggccc
      901 gcttatactc gtagaacagg ctccgtcaga cgctgtggtg acgacatgcc tcagacacgt
      961 tacagattct ctctgcaact tgtagcaatg taagtctctt ttgtgctatc ttcttctcgg
     1021 cggtcatact ctcaagcccc aaagttactc acttcctgac gaccattttt ggctcaattt
     1081 atcgcgacca gaaacagttt gcgaggggag aggtatttct atgcgtcata gaagacgcag
     1141 ggtatgctta tggcgatgtc gccgaaaagg tcgaaggcct tacggcgcgg aggtcttgat
     1201 aaccctgaca ccgacatgac cggggtgatg aaagcgtctc aggggagcgc cgccttgagg
     1261 cccctggcga aggagaagac tacgtcggca aggtcgcgcc tgccgctgtt ttcttcgatc
     1321 agcttgacga aggcgctgcc tactacgacg ccgtcggcga gaggcgcgat ccggcgggcc
     1381 tgctcgggcg tcgagatccc gaaaccgacg gcaacgggga ggtcgctcct tttgcggatt
     1441 cttgcaacgt cacggctgat gtcctcgatt tccggcctcg ccgttcccgt gacgccggtg
     1501 acggagatgt aatagataaa cccggaggcc ttttttgcga tttcttcgac ccggtcgttc
     1561 ccggtcgtcg gggcgacgag ggagatgaaa tcgatcttct ccctgtccgt gtaccgccgt
     1621 agttcccccg attcttcggg aggcaggtcc acgacgagta tcccgtctac cccggcctcc
     1681 ttcgccttca gcgcgaacct ctcgtttccg taagcgaaaa tcgggttgta ataaccgaag
     1741 aggacgatgg gtatttcgct cgccctgcgc agggccgcga ccatgtcgag gatggcgtcg
     1801 agggtgaccc cgttcttcag tgcccgctgc gacgcggcct ggatgaccgg gccgtccgcc
     1861 gtcgggtcgg agaagggcac accgacctcc aggatatcga caccggcctt gtcgagggca
     1921 aggaggacct cttccgtttt ctccagcgac gggtcgcctg ccgtgacata ggcgataagg
     1981 gccttttcat ttttcgcccg gagcgaccgg aacgcctttt cgatccgctt catgtctgtc
     2041 ttccttccat gctttcggcg atcacggccg cgtccttgtc tccgcggccc gaaaggttga
     2101 tgacgatgat cctgtccctg tcgagtccgg gggccagctt catggcgtag gcgaccgcgt
     2161 gggcgctctc catggcgggt atgattccct cgaggtgcga gagctccatg aatgcctgta
     2221 cggcctcatc gtcagtgacg ttgacgtacc tggcccggcc ggtcgcatgg aaatagccgt
     2281 gttccggccc cacgccggga tagtcgagtc cggccgcgat ggaataggcg tcccgtacct
     2341 ggccggagtc gtcttggagg agataggtct tgttgccgtg gaggaccccc accctgccgg
     2401 ccgagatgct cgcggcgtga agccccgtgg cgatcccctt gccggctgct tccacgccga
     2461 ccagggacac cgccgtgtct ttcctgaagg ggtaaaagag acccatcgca ttgctcccgc
     2521 cgcccacaca ggcgatgaga taatcgggga gccttccttc ctgcctcagg atctgtcttt
     2581 ttgtttccct tccgatgacg ctctggaagt tgcggaccat catcgggtac ggatggggcc
     2641 ccgccgtcgt gccgatcacg taaaaggtgt cgcgcacgtg ggcgacccac tggcgcatcg
     2701 cctcgttcat ggcgtctttc agcgtcgccg tccccgaatg gaccgggacg acct
//
LOCUS       DAOTMX010000246         2739 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_28913_length_2739_cov_3.032787, whole genome shotgun sequence.
ACCESSION   DAOTMX010000246 DAOTMX010000000
VERSION     DAOTMX010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28913_length_2739_cov_3.032787"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1582
                     /locus_tag="P5573_07080"
     CDS             <1..1582
                     /locus_tag="P5573_07080"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HRT27860.1"
                     /translation="QLMLGYLPMLHHPDPKKVFIVGLGSGATAKAVLDFPGLRSALCA
                     ELEPAVLEAVGFFAAVNNRVQDDPRFKVRIDDGRNALLASKEKCDVIISEPSNPWIAG
                     VANLYTTEFYQVGKARLNEGGVFCQWFHLYSMNPADVKMVLKTFFSVFPRGTVWTTAG
                     ADIILLGSESEPAFDYARWNELFQNSPSVIKSMQAIKMTAPDTVFSYYLADSKTLFPL
                     YKNAYLNTDDRPWLEFSAPRNLYEPTTPVNMRGLAKYRGGPLPPVRGYPAGRPLSTEY
                     YLAAVRQAEAVGDAVLLEKALEEALRIHPTDFELRMFKAQRLIRAGKTMKGERELREI
                     VADNPGKRAGYAALARLYRDQGFPERAEPLYEKAVRMDTGDEELNGEFIAFLRKQKRL
                     DKAAALLEELRKEHPDAARYQFLSGLLRMDGKDYAGASKFFEAALEKKPGSYEVMKNL
                     LAAYREMGDNEKVIRLGEELVKKNPYDREAVVDFGKALMKSGEVKRGREMLVRAMQLN
                     PYENREIEEFLEGRIARP"
     gene            complement(1948..2559)
                     /locus_tag="P5573_07085"
     CDS             complement(1948..2559)
                     /locus_tag="P5573_07085"
                     /inference="COORDINATES: protein motif:HMM:NF020757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglII/BstYI family type II restriction
                     endonuclease"
                     /protein_id="HRT27861.1"
                     /translation="MKIAETYSHLNGLEFLLVHKPSLWKEIQSVVASVDADKCRTKIS
                     KEKTMKGRILFSPIDMNAAFSQLLRKQSWNESRVSYWVTKNEKLIRKTIAMSAEEQKK
                     EIEAAGETPIFSYNQTDFVKDRVAIEVQFGKYAFVAYDLFVKHLAFYVGDRIDVGIEI
                     LPMKSLQSQMSSGVAYYEGEFYNVVRQGRGVPAVPLILVGIEP"
     gene            complement(2559..>2739)
                     /locus_tag="P5573_07090"
     CDS             complement(2559..>2739)
                     /locus_tag="P5573_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27862.1"
                     /translation="LARQRIRQEMEGTLRTRPMDRPVYDPVEAGNSLTAVPWKRKDPK
                     QMTLFEKRTKYRKER"
BASE COUNT          638 a          746 c          761 g          594 t
ORIGIN      
        1 acagttgatg ctcggctatc taccgatgtt gcatcacccg gacccgaaaa aagtattcat
       61 agtcggcctc ggaagcggtg cgacggcgaa ggcggtcctg gacttccccg ggctccggtc
      121 cgctctctgc gccgagctgg aaccggccgt cctcgaggcg gtcggcttct ttgccgccgt
      181 caacaaccgc gtccaggacg acccgcgatt caaagtaagg atagacgacg gacggaacgc
      241 cctcctggcc tcgaaggaaa agtgcgacgt gataatctcc gagccttcga acccgtggat
      301 cgccggtgtt gcgaaccttt acacgacgga gttttaccag gtcgggaaag cccggctcaa
      361 tgagggcggc gtcttttgtc agtggttcca tctgtatagc atgaacccgg cggacgtgaa
      421 gatggtgctg aagaccttct tctcggtctt cccccgcggt accgtgtgga ccaccgccgg
      481 cgccgacatc atcctcctgg gctccgagtc cgaaccggcc ttcgattacg cgagatggaa
      541 cgagctcttc caaaacagcc cgtcggtgat taaaagcatg caggccataa agatgacggc
      601 ccccgacacc gtcttttcct attatctggc cgattcgaaa accctctttc ccctgtacaa
      661 gaatgcctac ctgaacacgg acgaccgtcc ctggctcgag ttttcggcgc cccggaatct
      721 ctatgaaccg acgacgccgg tcaacatgag ggggctggcg aaatacaggg gagggccgct
      781 tcctccggtc aggggttatc ctgcgggcag gccgctttcc accgaatact acctggccgc
      841 agtccggcag gcggaagcgg tcggggatgc cgttctgctg gaaaaggcac tggaagaggc
      901 gctgagaatc catccgactg acttcgagct gaggatgttc aaggcgcagc gcctgatccg
      961 ggcggggaag accatgaagg gtgagcggga acttcgggag attgttgccg ataaccccgg
     1021 caaacgtgcc ggctacgcag cgctcgcccg attatacagg gatcagggat tcccggaacg
     1081 ggccgaaccg ctgtacgaga aggcggtccg catggacacg ggcgacgagg agttgaacgg
     1141 agaatttatc gccttcctca gaaaacagaa gaggctcgac aaggccgccg cgctccttga
     1201 agaactgcgg aaggaacatc ccgacgcggc gcgttatcaa ttcctgtcgg gcctgttgcg
     1261 gatggacggg aaggactatg ccggggcctc aaagttcttc gaagcggccc tcgagaagaa
     1321 accgggcagc tacgaggtca tgaaaaatct gctcgctgcc taccgggaaa tgggcgacaa
     1381 cgaaaaggtc atccgcctcg gtgaggagct tgtaaagaaa aacccctatg accgggaagc
     1441 ggtcgtagat ttcggcaagg ctttgatgaa gtccggcgag gtgaagagag gccgcgagat
     1501 gctcgtccgg gccatgcagt tgaatcctta cgagaatcgt gagatagaag agttcctgga
     1561 ggggagaatt gcgcgccctt agacattccc cggcactgag gagggtttcc tatgccagat
     1621 acgaatagcc ggtatcgaag aaaggaatgg cctaaaaatc gtcatgatca agaatattca
     1681 gtaatttgcc cgggcggtgg agacgaagaa gcaactggag aatcacgaaa gggaacttcg
     1741 ggcctccggc aggaattgac cgaagtccgg tcggagttga acacactgaa aaagtccttc
     1801 acctcggaac actgcggtat cgcagaacaa gagggagcgc atagacaaga gacgcttcta
     1861 tactatgaat aacaacaagc tcacctgttg ctatcccgct ggcgctccat ggcggtagat
     1921 ggagcgtcct gttagatcgc catgttttca cggttcaatg ccgacaagaa taagcggaac
     1981 cgccggcaca ccacggcctt gacgaactac attgtaaaac tcgccttcat agtaagcgac
     2041 tccggaactc atctggcttt gaagggattt catcggaaga atttcaattc ctacgtcgat
     2101 tcgatcaccc acatagaatg cgaggtgctt gacgaaaagg tcatacgcaa caaacgcata
     2161 ctttccgaac tggacctcaa tggccacgcg atccttgacg aaatcggtct ggttatagct
     2221 gaagataggc gtctctccgg ctgcctctat ctcctttttt tgctcttccg ctgacatcgc
     2281 aatcgtctta cgaatcagct tttcattctt ggtaacccag tagctcaccc gactttcgtt
     2341 ccaggattgt ttccgaagta gctgactgaa tgctgcgttc atatctatgg gactgaatag
     2401 gatcctgccc ttcattgtct tttctttgga aattttggtt cggcacttgt cggcatccac
     2461 tgacgctacg actgattgaa tctctttcca aaggctcggt ttgtgtacaa gaaggaactc
     2521 aagtccattt agatgagaat acgtctcggc gatcttcatc aacgttcctt tctatacttt
     2581 gtcctctttt caaacaaggt catctgcttc ggatcctttc tcttccaggg aacggctgta
     2641 aggctgttcc cggcttccac agggtcatac acaggcctgt ccatggggcg ggtccgcaat
     2701 gtgccttcca tttcctgccg aattctctgt cgtgcaagt
//
LOCUS       DAOTMX010000247         2719 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_29164_length_2719_cov_3.193318, whole genome shotgun sequence.
ACCESSION   DAOTMX010000247 DAOTMX010000000
VERSION     DAOTMX010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29164_length_2719_cov_3.193318"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="P5573_07095"
     CDS             complement(<1..319)
                     /locus_tag="P5573_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27863.1"
                     /translation="MRTDCGALDGQVRRLQEMLRRCALCPRKCGVNRAVGERGYCALD
                     HRIVLSHALPHHGEEPPLSGNRGAGAVFFSSCNLQCIYCQNHQISHTLRGRCVGAAEL
                     AGLM"
     gene            complement(319..1392)
                     /locus_tag="P5573_07100"
     CDS             complement(319..1392)
                     /locus_tag="P5573_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27864.1"
                     /translation="MRDLCIRAGRKVYRIIKDGGFDLDRVTTYLAPAGGPRWLAASGF
                     DLALLENAVLGRSKPVLLAGSSSGAWRMAAWLQPEPAKSYRTFMETYLKDSSYRRSDT
                     PETLLDSMRALLDAYIEDDAVPFALAHRRYRLAVTTARAKNFAASETVSIQRLGVGLA
                     WLLNALSPDYLRFIFERVVFYSGALPPRFCLRHGFCGKTIPLDATNFKAAILASGAIP
                     VVIAGVRDIYGAPRGIYRDGGVFDYHLNHEYTAREGDVTLLFNHMERIVPGWFDKGLK
                     SRRPSAKILENVLLVHPGDGLVERLPGRRVPDREDFVTFIDDPKTRIKNWRKAVDLCA
                     PLGEQFLEAVASGRIRDVVEVLE"
     gene            1578..>2719
                     /locus_tag="P5573_07105"
     CDS             1578..>2719
                     /locus_tag="P5573_07105"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HRT27865.1"
                     /translation="MDIKTQVLEIAAGAKKASRVLAGAPSPAKDASLLRMAEELVKNT
                     EFLLDENGKDVRYAREKGLSSAMVDRLTLKAETVSGIARGLREVAALPDPVGKVTSLW
                     KRPNGLVVGRMRIPLGVIGIIYESRPNVTADAAALCLKSGNTVILRGGSEAIHSNLAI
                     AGILRRVLKENGLPEAAVQIIPTTDREAVHELLQLEEYIDLIIPRGGEDLIRAVVQTS
                     RIPVIKHYKGVCHIYVDAGADLDMALDICMNAKVQRPGVCNAMETLLVHKDIAQTFLP
                     LMAKRYSDAGVLLKGCAETRKILPGLEEAKDEDWYAEYLDLILAVRTVGGLDEAIEHI
                     EKYGSLHTEAIITRDYRNAQRFLNEVNSSTVLVNASTRFSDGFELGL"
BASE COUNT          583 a          850 c          798 g          488 t
ORIGIN      
        1 gcatcaggcc cgcgagctcg gcggcgccga cgcaacggcc ccgcagggtg tgactgatct
       61 ggtggttctg acagtagatg cactggaggt tgcaggaaga aaagaagacc gccccggcgc
      121 cgcggttccc cgataaaggc ggctcctcgc cgtggtgggg cagggcgtga ctgaggacta
      181 tccggtggtc gagggcgcag tacccccgct caccgaccgc cctgttcacc ccgcatttcc
      241 ggggacagag ggcacagcgc cgcagcatct cctgcagacg ccgtacctgc ccgtctaagg
      301 caccgcaatc cgttctcatc actcgagcac ctctaccaca tcgcggatac ggccgcttgc
      361 gaccgcctcc aggaattgct ctccgagagg ggcgcacaga tccactgcct tgcgccagtt
      421 ctttatcctg gtcttcgggt cgtcgatgaa ggtgacgaag tcttccctgt cggggactct
      481 tctgccgggt aatcgttcca ctaatccgtc tccgggatgg accaggagga cattctcgag
      541 tattttcgca gatgggcggc gcgacttcaa ccccttgtcg aaccacccgg ggacgatgcg
      601 ttccatgtga ttgaaaagca gggttacatc cccttcgcgg gcggtgtatt cgtgattaag
      661 gtgataatcg aagacgccac cgtccctgta tatcccccgc ggtgcgccgt agatgtcacg
      721 gacaccggcg atgacgaccg ggatggcacc ggaggcgagg atcgccgcct tgaaattcgt
      781 ggcgtcgagc gggatcgtct tcccacagaa accatgccga agacaaaatc gcggcggcag
      841 agcgccgctg taaaagacga cgcgctcgaa gataaacctc aggtaatcgg gactcagggc
      901 attcaagagc caggccaacc ccactcccaa ccgctggatc gaaaccgtct ccgaagcggc
      961 gaaattcttc gcccttgcgg tcgtgacggc gagtcggtac cgcctgtggg ctagggcgaa
     1021 ggggaccgca tcatcctcga tatacgcatc caagagcgct ctcatggaat cgagcagcgt
     1081 ctccggggtg tccgaccgcc tataagacga gtccttcaag taggtctcca tgaaggtcct
     1141 gtaactcttg gcgggctcgg gctgcaacca ggccgccatc ctccaggcgc ccgacgacga
     1201 cccagcgagc agcacgggct tggagcgtcc gaggacggcg ttctccaaga gcgcgaggtc
     1261 gaaaccgctt gccgccagcc agcgcggccc gccggccggc gccaaatagg tggtgacccg
     1321 gtcaagatca aacccaccgt ctttgatgat ccgatagact ttgcggcctg ccctgatgca
     1381 caggtctctc atttccgcct ctctctgtac cacacccctt tgcggacttc gaaaaaaagt
     1441 gagcatatct aacaaagatc actcaactta tcaagagggc ccccggaaat ttaatggcct
     1501 tgacacatcc ccgtaccttg ggtatgaaca ggcgagctga tagtttctca cccactggat
     1561 ggtagaaggg ggtaacgatg gacatcaaaa cacaggtctt ggagatcgcc gcaggcgcaa
     1621 aaaaggcctc ccgcgtgctt gccggtgcac cgtctccggc gaaagacgcc tcactgctga
     1681 ggatggccga ggaattggtc aaaaacacgg agttcctgct cgacgaaaac gggaaggacg
     1741 ttcggtacgc ccgcgaaaag ggtctctctt ccgcgatggt cgaccggctt accctgaaag
     1801 cggagaccgt tagcgggatt gccaggggcc tgcgtgaagt ggccgcactg cccgaccccg
     1861 tcgggaaggt gacttccctg tggaagaggc ctaacgggct agtcgtcggc aggatgcgga
     1921 tccccctcgg cgtgatcggc atcatctatg agtcccgacc caacgtgacg gccgacgcgg
     1981 ccgccctctg ccttaaatct ggaaacaccg tcatcctgag agggggctcc gaggccatcc
     2041 attccaacct cgccatcgcc ggcatcctcc ggcgcgtcct gaaagaaaac ggtctccccg
     2101 aagcggccgt ccagataatc ccgacgaccg acagggaggc cgtccacgag ctgctccagc
     2161 tcgaagagta catcgacctc atcatcccgc gggggggcga agacctcatc cgggcggtcg
     2221 ttcagacgtc gaggataccg gtcatcaaac actacaaagg tgtttgccat atctatgtcg
     2281 acgccggcgc agacctcgac atggcgctcg acatctgcat gaatgccaag gtccagaggc
     2341 cgggcgtatg caacgccatg gagaccctgc tggtccacaa agacatagcg cagacgttcc
     2401 tgcccctcat ggcgaaacgt tactccgatg ccggtgttct gttgaagggc tgcgcggaaa
     2461 cgagaaagat cctgcccggc ctcgaagagg cgaaggacga ggactggtac gccgaatacc
     2521 tcgacctcat actcgccgtc cgcacagtcg ggggccttga cgaggccata gaacacatcg
     2581 agaagtacgg ttccctccac acggaggcga tcatcacgag ggactaccgg aacgcacagc
     2641 gtttcttgaa cgaagtcaat tcttccacgg tactcgtcaa cgcctccacg cgtttcagtg
     2701 acgggttcga gcttggtct
//
LOCUS       DAOTMX010000248         2704 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_29381_length_2704_cov_5.115515, whole genome shotgun sequence.
ACCESSION   DAOTMX010000248 DAOTMX010000000
VERSION     DAOTMX010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29381_length_2704_cov_5.115515"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1057
                     /gene="mtnA"
                     /locus_tag="P5573_07110"
     CDS             <1..1057
                     /gene="mtnA"
                     /locus_tag="P5573_07110"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HRT27866.1"
                     /translation="VYWADDAVGMIDQKALPFEFRYFTLRRYQDVASAIKDLTVRGAP
                     AIGVAAAMGIALGVLHSTAGNREDLQREFCLICEEFARSRPTARNLFWAIERMRRTLE
                     ANLSAGTGLEELKNALVEEAVRVGEEDVAVNRQVGLNGRRFIKDGANILTHCNAGALA
                     TAGYGTALGVIRAAMEEGKKVHVYADETRPVLQGARLTCWELKEENIPATLITDSTAG
                     FLMSRRKVDLVIVGADRIAANGDTANKIGTYTLAVLAKENRVPFYVAAPLSTIDKDTE
                     KGEDIPIEERDGAEVVTIGGVRTAPEGIGAYNPAFDVTPARYVTAIITEAGVLRRPFE
                     ENIKKLPEASPYRGKIA"
     gene            1065..1682
                     /locus_tag="P5573_07115"
     CDS             1065..1682
                     /locus_tag="P5573_07115"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRT27867.1"
                     /translation="MKRLFLFDFDGVVVDSLELYEHSVNLCLEKLGLPPFRSRQEFLR
                     VFEDNFYEGIGKKGVDVPAFTKVSAELAPSLNYSKVTPVRELEPVLENLRKRHTLAIV
                     SSNSNYAIERILSGIGYGRFFDEILGSDFMFSKVEKIRHITERTGIQGNGTFYIGDTV
                     GDILEAKQAGVKTVAVTWGWHTKEQLAVANPDYLIDSPEELLDIG"
     gene            complement(1898..>2704)
                     /locus_tag="P5573_07120"
     CDS             complement(1898..>2704)
                     /locus_tag="P5573_07120"
                     /inference="COORDINATES: protein motif:HMM:NF014635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HRT27868.1"
                     /translation="GVEEDVIREEVNMDYRAFSEEISRMVRPQTFPLGVKLIKDADKL
                     PKEAARPAKFGVKIAVCQWTTMARRWGRVLGATAEDINCTACLAALGLKRLDDRRHLG
                     QYFLEMGFFDDIDMAEKFAKELELIKPGEISAVAYFPLDMAPIDPDVIVIYGTPAQMA
                     RLAAGYIHSSGELIELKSAFGLSCLAAIKPYFSKKPAFVIPGRGERILAGTDEGEMFF
                     TFPAEYAEQLLDGLRKTHEKGTRYPVQSYVIYEPPIIRPMKNLADKLSDS"
BASE COUNT          580 a          805 c          770 g          549 t
ORIGIN      
        1 cgtctactgg gccgatgacg ccgtcgggat gatcgaccag aaggcccttc ccttcgagtt
       61 tcgctacttt accctccgcc gttaccagga cgtcgcctcg gccataaagg acctcacggt
      121 ccgtggcgcc ccggctatcg gcgtcgccgc cgccatgggc atagccctcg gtgtcctcca
      181 ctcaaccgcc ggaaaccgcg aagacctgca gagggagttc tgtcttattt gcgaggagtt
      241 cgcccgctcc cgccccacgg cgagaaacct cttctgggcc atcgagcgga tgagacgcac
      301 cttagaagcc aacctctccg ccggcacggg cctcgaggaa ctgaagaacg ccctggtaga
      361 agaagccgtc cgggtcggcg aggaagacgt ggccgtcaac cgccaggtcg gcctgaacgg
      421 ccgccggttc atcaaggacg gcgcaaatat cctcacgcac tgcaacgccg gggccctggc
      481 cacggccggg tacggcaccg cgctcggcgt catccgggca gcgatggagg aaggaaagaa
      541 ggttcacgtc tacgcggacg agacgaggcc cgtcctccag ggcgcccgcc tgacctgctg
      601 ggaattgaag gaagaaaaca tcccggcgac gctcatcacg gacagcacag ccggtttcct
      661 gatgagcaga agaaaagtcg acctcgtcat cgtcggcgcc gaccggatcg cggcgaacgg
      721 cgatacggcg aacaagatcg gaacttacac gctggccgtg ctcgcgaagg aaaacagggt
      781 tcccttctac gttgcggccc ccctttccac gatcgacaag gatacagaaa aaggggaaga
      841 catacccatc gaagaaaggg acggggcgga ggtcgtcacg attggcggcg tgaggacggc
      901 gccggaggga atcggagctt acaatcccgc cttcgacgtc acgcccgccc ggtacgtgac
      961 ggccatcatc accgaggccg gggtcttacg caggcccttt gaagaaaaca taaaaaagtt
     1021 gccggaggca tcgccgtacc gaggaaagat tgcgtgaacc ggacatgaaa aggctctttc
     1081 tcttcgattt tgacggagtg gtcgtcgact ccctggaact ctacgagcac tcagtcaatc
     1141 tctgcctcga gaagctgggg ttaccgcctt tcaggagcag gcaggagttc ctccgagtct
     1201 tcgaagacaa tttttacgag gggatcggca agaaaggggt ggatgtccct gccttcacga
     1261 aggtttcggc ggagcttgcg ccttcgctca actacagcaa ggtcactccc gtgagagagc
     1321 tcgaacccgt cctcgagaac ctccgcaaaa ggcacaccct ggccatcgtt tcgagcaact
     1381 cgaattacgc catcgagcgc atcctttcgg gaatcggcta cggccgtttt ttcgacgaga
     1441 tcctgggcag cgactttatg ttcagcaagg tcgagaagat ccgccacatc acggaacgga
     1501 ccggtattca gggtaacggc acgttctaca tcggcgacac ggtcggggac atcctggaag
     1561 cgaagcaggc gggcgtcaaa acggtcgccg tcacctgggg ctggcacaca aaggagcagc
     1621 tcgcggttgc gaaccccgat tatctgatcg actctccgga agaactcctc gatatcggct
     1681 gaagagaaac cgtgtcttcc gaccttgcgc gagttcgtca tccaattctt gatggattca
     1741 taaaaagtcg cgaattacgc gtttcgtcat tccggcgaac gccggaatcc agtatattca
     1801 atatgttctg gatgcccccg tatcgagtac ggggcaggct taatcaagtc cggcatgacg
     1861 tttttggact ttttacgggg acatctattc ttgacggtca ggagtcggac aacttatcgg
     1921 caagattctt catcggcctg atgatcggcg gctcatagat tacatagctt tgcacagggt
     1981 accgagtccc cttttcatgg gtcttcctca ggccgtccaa aagctgctcg gcatattctg
     2041 ccgggaaggt gaagaacatc tcgccctcat ccgtgcccgc caagatccgt tccccccgtc
     2101 cggggatgac gaaggcgggt tttttgctga aatacggctt tatggccgca aggcatgaga
     2161 gtccgaatgc cgatttcagt tcgataagct cccccgagct gtggatgtaa ccggcggcca
     2221 gacgtgccat ctgcgccggc gtcccgtaaa tcacgattac gtctgggtcg ataggcgcca
     2281 tgtcgagcgg gaagtaggct actgcgctta tttcccccgg ttttatgagt tccagttctt
     2341 tcgcaaactt ttcggccata tcgatgtcgt cgaaaaaccc catctccaga aagtactgcc
     2401 cgaggtgtcg gcgatcgtcg agtcgtttta ggccgagcgc cgcaaggcag gctgtgcagt
     2461 tgatgtcttc cgctgtcgct ccgagaaccc gcccccaccg cctcgccatc gtcgtccact
     2521 ggcaaaccgc aatcttaacg ccgaattttg cgggtctcgc cgcttccttc ggaagcttgt
     2581 cggcgtcttt gatcaatttt actccgaggg gaaaagtctg gggacggacc atccgggaaa
     2641 tttcttcgga aaatgctctg taatccatgt tgacctcctc tctgatgacg tcttcttcca
     2701 cccc
//
LOCUS       DAOTMX010000249         2683 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_29678_length_2683_cov_3.713851, whole genome shotgun sequence.
ACCESSION   DAOTMX010000249 DAOTMX010000000
VERSION     DAOTMX010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29678_length_2683_cov_3.713851"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="P5573_07125"
     CDS             complement(<1..653)
                     /locus_tag="P5573_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27869.1"
                     /translation="MKDERSTLKFSEFLKKDLKKTDQVEDLEKEEGGPERQAESLPED
                     FELFRKLKELRLGGGESAPSKKDSATFDSYKMAERIRATELIVKKEVQDNVSGSDRLR
                     EKSDEDDGRASVAKADVEGPTPGADRLGKAGVDVAGVEEHEAVYLKAIEYLQSVRFEV
                     SENHPFDVEPAVEIVRMVIDSPELLKRSYALTFKHDDAGSLESHQVNVMIYALKVGMG
                     "
     gene            990..2312
                     /locus_tag="P5573_07130"
     CDS             990..2312
                     /locus_tag="P5573_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRT27870.1"
                     /translation="MKKIFYNWRRPAYALALVIGLICFAVGFNGDSRVIALEQSTYKN
                     LKLFNEAMDIIEKNYVEKVDPQTLLQGAINGMVKSLDPHSMFLTAEMYKELQTETRGS
                     FGGIGIEITILNDVLTVVSPIEDTPAYNAGIKPGDQIVRIDDKPTKGISILDAVKKLR
                     GPENTKVKLTIMRKDAAQPKDYTITRAVINIKSVRHNVYEGRIGYIRVSSFQESTAEE
                     LKKSLLEIKKKAGVLDGIVLDLRNNPGGLLDQAVKVSDIFLKSGAIVSIKGRVKNLEN
                     TFTARDDGDEPTCPIVVLVNEGTASASEIVAGALQDNGRAVILGTQTFGKGSVQTVVP
                     FEDGSALKLTTAKYFTPKGRSIQAEGITPDIRLEFARAPEEKDHETVREKDLKGHITG
                     DEEKAEKARLPKKAPDDLSKDNQLKGAVDLIKSWKIFEKMNSKGSFGR"
     gene            2309..>2683
                     /locus_tag="P5573_07135"
     CDS             2309..>2683
                     /locus_tag="P5573_07135"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HRT27871.1"
                     /translation="MKKRKPVFEYKRVLAVLLLLALSTVVFVFINDMRERGISKPIED
                     KSRSGKKVRESISVSEKTPTKKTRRVAIVIDDIGYDLSQVDELLKIRAPLTFAVLPHC
                     QHSQEAAERIHRAGKEVLLHLPM"
BASE COUNT          699 a          741 c          653 g          590 t
ORIGIN      
        1 cccatgccga ccttcagcgc gtaaatcatg acgtttacct ggtgggactc aagacttccc
       61 gcgtcgtcgt gcttgaaggt aagggcgtat gacctcttca gtagttccgg agaatcgatg
      121 accattctca cgatttcgac ggccggttcg acgtcgaacg ggtgattttc gctcacctcg
      181 aacctcaccg actgaaggta ctctatcgcc ttcaggtaga ccgcctcatg ttcctccacc
      241 ccggcgacat cgaccccggc cttacccagc ctatccgccc ccggtgtcgg tccttcgaca
      301 tcggccttcg ctacagacgc ccgaccatcg tcttcatcac ttttctcccg caaccgatca
      361 cttccgctta cgttgtcctg cacttctttt ttgacgatga gttcagttgc gcgtatcctc
      421 tcggccatct tgtaagaatc gaaggtcgcc gaatctttct tcgaaggtgc gctttcgcct
      481 ccgccgagac gaagttcttt taattttcgg aaaagctcaa aatcttcggg taacgactcc
      541 gcttgacgct ccggcccgcc ttcctctttt tcgaggtctt caacctggtc tgtttttttt
      601 aaatctttct tcagaaattc tgaaaacttt aaggtcgacc tttcgtcctt catgaccctt
      661 cctttactca atccttgcaa ctcgtcgtcg ccggtcaatc gcccctgtca atcgcaagga
      721 tgccatcctc ggaaaaaccc ggcgattgct ctccggcccg cttttcaaat attaatcgat
      781 acatttaaat gagatggtct caataatcaa caatatcttt atacaaactg gcgccgggcc
      841 cgtcaacact gtcatattct cccccaccct gttttacgct atgattttct gcaagaaggg
      901 ataaattcca aaaaatatcg atgtaaattt atctgaaaga tggtatcctg ctatgtcaca
      961 tcatgaagaa tcttatcccg gaggatcgga tgaaaaagat tttttataac tggcgaagac
     1021 cagcttatgc attagctctt gtaataggcc tcatttgctt tgcggtcgga ttcaacggcg
     1081 acagccgggt cattgccctc gagcagagca cttacaagaa cttgaaactc ttcaacgagg
     1141 cgatggacat catcgaaaaa aactacgtgg agaaggtcga cccccaaaca ctgctgcaag
     1201 gtgccataaa cggcatggtc aaatccctgg acccgcacag catgttcctc accgcggaga
     1261 tgtacaagga attgcagacg gaaacaagag gaagctttgg cggaatcggc atcgaaataa
     1321 cgatcctgaa cgacgtactc actgtagtct ctcccatcga agatacccct gcctacaacg
     1381 ccggaatcaa gcccggcgat cagatcgtta ggatagacga taaaccgacg aagggcatct
     1441 ctatactgga cgccgtaaag aaactgcgcg gccccgaaaa cacaaaagtc aagctcacca
     1501 taatgagaaa agacgccgcc cagccgaagg actacaccat cacgagggcc gtcatcaaca
     1561 taaagagcgt ccggcacaat gtctacgaag gtcgtatcgg ttatatccgc gtctcctcct
     1621 tccaagagag cactgccgag gaattgaaga aatcgctcct cgagataaag aagaaagccg
     1681 gcgtactcga cggtatagtc ctcgacctcc ggaataatcc cggaggcctt ctcgaccagg
     1741 ccgtcaaggt ctccgatatt tttctgaaaa gcggggcgat cgtatcgata aagggccgag
     1801 tcaaaaacct cgaaaacaca ttcaccgcga gggacgacgg cgatgaacct acatgcccga
     1861 tcgtcgttct cgtgaacgaa ggcacggcaa gtgcctccga gatcgtggcc ggtgcattgc
     1921 aggacaacgg aagggcggta atcctcggta cacagacctt cggtaagggg tctgttcaaa
     1981 cggtggtacc cttcgaagac ggctcggcgt tgaaactcac caccgctaaa tattttacgc
     2041 ccaaagggcg gtctatccag gccgagggga tcactccgga catccgcctc gaatttgcca
     2101 gggcaccgga ggaaaaggac catgagacgg tccgggagaa agacctcaaa ggtcacataa
     2161 ccggtgatga ggagaaggca gagaaggcgc gattgccgaa gaaagccccc gacgaccttt
     2221 cgaaggacaa ccagctcaaa ggcgccgttg acctgatcaa aagctggaag atcttcgaga
     2281 agatgaacag taagggttca ttcgggaggt gaagaaaaga aagccggttt tcgagtacaa
     2341 gagagtcctg gcagtactcc tcctgcttgc gctgagcacg gtggtctttg tattcatcaa
     2401 tgatatgcgc gagaggggaa tcagcaaacc gattgaggac aaaagtcggt cgggaaagaa
     2461 ggtacgggaa agtatcagtg tttcagaaaa gacgcccaca aagaagaccc gtcgggtggc
     2521 gatagtcatc gatgacatcg ggtatgacct ttctcaggtc gacgaacttc tcaaaatccg
     2581 ggcgcctctg acattcgccg tcctgcccca ttgccagcat tcccaggagg cagcggagag
     2641 gatacaccgg gcagggaaag aggtcctcct ccacctcccc atg
//
LOCUS       DAOTMX010000250         2658 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_30041_length_2658_cov_3.488283, whole genome shotgun sequence.
ACCESSION   DAOTMX010000250 DAOTMX010000000
VERSION     DAOTMX010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30041_length_2658_cov_3.488283"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1155
                     /locus_tag="P5573_07140"
     CDS             <1..1155
                     /locus_tag="P5573_07140"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HRT27872.1"
                     /translation="EAAKTPNMDRVAREGTVGLIDTVPRGFPPGSDVANLSVLGYDPK
                     VCYSGRGPLEAASMGVRLEPEDVAYRCNLVYLRKDNGATVMDDYSAGHITSEEARELI
                     TDLENRLGGDGLHFYPGVSYRHLLVWRGGPEAVETTPPHDITGRDVRNHLPTGTGSEE
                     VMRLIEKSRAVLEGHRVNRERLSAGRKPANSIWLWGQGHSPAMEPLTEKYGLRAAMIS
                     AVDLLKGIGICAGFDILNVPGATGYIDTNYAGKAQSALQALKDRDFVFVHVEAPDEMG
                     HAGNVEGKIRAIEDFDEKVVGVVLAGMEEFGDFRIMVLSDHPTPISRKTHMGDPSPFA
                     VYSSLGENLGHGESFCERAAEKSGILVTPGHRLMDGFIRDWRAFIEKESR"
     gene            1136..1537
                     /locus_tag="P5573_07145"
     CDS             1136..1537
                     /locus_tag="P5573_07145"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRT27873.1"
                     /translation="MKKKAVELRVTYADTDAMGVVYHTNYIKWFEVGRTEYMRDLGVV
                     YSELEKEGYYLPVTEVYCHYLSPARYDECVQVVTTINFFRRASVRFDYEIYDANGAVM
                     LAEGYTAHAFINREGKIVRAPKHVTEKIGTL"
     gene            1548..2237
                     /locus_tag="P5573_07150"
     CDS             1548..2237
                     /locus_tag="P5573_07150"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRT27874.1"
                     /translation="MAKIDEKGLKTPNLFLVKARRTLDYLIENRKRVFLAVGVAVGVV
                     LIAVSWYLYRLDYEGKAQKLYAAAFNYYHSAQTGYAGDAAVPQSVEKYREVVEKFPGT
                     KAAGLALFSLGNVYYRVADYDRAVEAFQDFLKSSAARKELKSLAFSGLGYCYEAKGQL
                     DKAVESYESAAKDPISSAFSGMTYMNIGRVCEKSKDPKKALDYYMKASEQKNDGLVDA
                     LVKRKIAELSS"
     gene            2275..>2658
                     /locus_tag="P5573_07155"
     CDS             2275..>2658
                     /locus_tag="P5573_07155"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HRT27875.1"
                     /translation="MKTIMSGNEAIARGAFECGATFAAGYPGTPSTEIMEQFSQYEGV
                     YAEWSPNEKVAVEVAVGAALGGAKAMAVMKHVGVNVAADPLFTVSYTGTNGALVVVTA
                     DDPSLHSSQNEQDNRHYARFAKIPML"
BASE COUNT          596 a          734 c          837 g          491 t
ORIGIN      
        1 gaggcggcga agacgccgaa catggacagg gtcgcccgcg agggcacggt cgggctgatc
       61 gacacggtcc cccgggggtt cccgccgggt agtgacgtcg ccaacctctc cgtcctcggt
      121 tatgacccga aggtatgcta cagcgggcgc gggccgttgg aggccgcgag catgggtgtt
      181 cgcctcgaac cggaggacgt ggcctatcgg tgcaacctcg tgtacctccg gaaggataac
      241 ggcgcgacgg tgatggacga ttactccgcc ggccatatca catcggaaga ggcgagagag
      301 ctcatcacgg acttagaaaa ccgcctcggt ggagatggcc tccacttcta ccccggcgtc
      361 agttaccggc atctcctcgt gtggcggggg gggccggagg ccgtggagac aacgccgccc
      421 catgacataa cgggacggga cgtgaggaat cacctgccta ccggaaccgg ctccgaagag
      481 gtgatgaggc tgatcgagaa atcccgtgcc gtcctcgaag gccacagggt caacagggag
      541 cgtctgtcag cgggccgaaa accggcaaac tctatctggc tctgggggca aggccattcc
      601 ccggccatgg agccgctcac cgaaaaatac gggcttcgcg ccgccatgat atcggccgta
      661 gacctgctga aaggcatcgg catctgcgcc ggtttcgaca tcctgaacgt cccgggagct
      721 acgggctaca tcgacacgaa ctatgccggg aaggcgcaga gtgcgcttca ggcgctgaaa
      781 gacagggact ttgtcttcgt ccacgtggag gcgccggacg agatgggcca tgccggcaac
      841 gtcgagggga aaatcagggc gatcgaagat ttcgacgaga aggtcgtcgg cgtcgtcttg
      901 gcggggatgg aggaattcgg tgacttccgg atcatggtgc tgagcgacca tccgacgccg
      961 atcagccgta agacccacat gggcgacccg agccccttcg ctgtatattc ttccctgggc
     1021 gaaaaccttg gacatgggga atctttttgc gaacgggccg ccgagaaatc ggggatctta
     1081 gtcacccccg gtcatcgatt gatggacggc ttcatccgtg actggagggc tttcattgaa
     1141 aaagaaagcc gttgaactgc gggtgaccta tgcagacacc gatgccatgg gggtcgtcta
     1201 ccacacgaat tacatcaagt ggttcgaggt cgggcggacg gaatacatgc gggacctcgg
     1261 cgtcgtctac tcggaactgg agaaggaagg ttattatctg cccgtgacag aagtatattg
     1321 tcactacctc tcaccggcca ggtacgacga atgcgtccag gtggtgacga cgataaactt
     1381 tttccgccgg gcgagcgtca gattcgacta cgagatatac gatgcgaacg gcgccgtcat
     1441 gctggccgag ggctataccg cccacgcatt cataaaccgt gaaggaaaga tcgtcagggc
     1501 acccaagcac gtgacggaaa aaatcggcac cctttgaggt tcatcagatg gcaaaaatcg
     1561 acgaaaaggg actaaagaca cccaacctct tcctggtcaa ggcccgtcgt accctggact
     1621 atctcatcga aaaccgcaag cgcgtctttc tggccgtcgg ggtggcggtc ggtgttgtcc
     1681 ttatcgctgt ttcctggtat ctgtaccgcc tcgactacga aggcaaggcc cagaagttgt
     1741 atgccgccgc tttcaattat taccattccg ctcaaaccgg ttatgccggg gacgccgcgg
     1801 tgccccagtc ggtcgagaag taccgcgagg tcgtggagaa atttcccggc acaaaggccg
     1861 ccggcctcgc tctcttcagt ctcgggaatg tttactatcg ggttgcagat tacgaccgtg
     1921 cagtcgaggc attccaggat ttcctgaagt cttccgcagc gcgcaaggag ctgaagtcac
     1981 tggcattcag cggcctcggt tactgctatg aggcgaaagg ccaattagac aaggccgtcg
     2041 aatcttacga gagtgcggcc aaggacccga taagcagcgc cttcagcggc atgacctaca
     2101 tgaacatcgg gcgggtctgc gaaaaatcga aggacccgaa aaaggcgctc gattattaca
     2161 tgaaggcctc tgagcagaag aacgacgggc tggtcgatgc cttggtcaag aggaagatcg
     2221 ctgaactgag ctcctgaaaa aatacagtgg atagatgtgg atagataagg tgcgatgaaa
     2281 acgatcatgt cggggaatga agcgattgcg aggggtgcct tcgagtgcgg ggcgactttt
     2341 gccgccggtt acccggggac gccgagcacg gagataatgg agcagttttc gcagtacgaa
     2401 ggcgtctatg ccgagtggtc gccgaacgaa aaggtggccg tcgaggtggc cgtcggggct
     2461 gccctggggg gcgctaaggc catggcggtc atgaaacacg tcggcgttaa cgtcgccgcg
     2521 gacccgctct ttaccgtgag ctataccggg acgaacggcg ccctcgtcgt cgtcacggcg
     2581 gacgatccgt cgctccacag ctcgcaaaac gaacaggata accgccatta cgcgcgattt
     2641 gccaagatac cgatgctc
//
LOCUS       DAOTMX010000251         2651 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_30147_length_2651_cov_3.643297, whole genome shotgun sequence.
ACCESSION   DAOTMX010000251 DAOTMX010000000
VERSION     DAOTMX010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30147_length_2651_cov_3.643297"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="P5573_07160"
     CDS             complement(<1..609)
                     /locus_tag="P5573_07160"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HRT27876.1"
                     /translation="MLTEFHDALKDPAAYGKRLKEESGRKVVGYFCSYTPEEMIYAAG
                     AHPFRLFGTGDGIHLADAHFQSYCCSLVRGALEGALAGRFDFLDGVVFPHTCDSIQRL
                     SDVWRVNMHGHFHLDVVLPVKLNTESAKTYMTDVLEKFRSDLEIKLGASISEDDLKKA
                     VRTFNDIRGSLKRLYELKSENPGIVSGADLYAVVKASMIMDRD"
     gene            complement(603..2324)
                     /locus_tag="P5573_07165"
     CDS             complement(603..2324)
                     /locus_tag="P5573_07165"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRT27877.1"
                     /translation="MYEKKPWLKFYGDIPESIDYPRVTMYEAVMKTVERLPGAVAYDF
                     LGYTASYRQFGNEIDRCADALASLGMKKGDRIAISMPTTPQGIICFYAANKLGAVAAM
                     IHPLSTPKEVEFYLNSSRSRFALTLDAFYGKFAGLREKTGLETLILTRISDYLTGLKK
                     IGFMLTKGRKIPKVPDDPAVRWWSDLMKTAHPPAPRADMHTDEMAVILYSGGTTGVPK
                     GIMLSNMNFISEGMQVSAVGRLSEKDAILAILPIFHGFGLGVCVNACFMGGAKSILVP
                     QFTPGTVAKLIRQKRPTFVIGVPTLFDALSRNPELQKADLSCLKATFSGADTLPRTVK
                     ERFESVVKQRGGTCRLLEGYGLTEAVTAIMVTPFDEYREGGIGVPFPDMSAKVVKLGT
                     TEEAPVGEEGEICLHGPAVMLGYLDNPEETAQALKTHPDGKVWLHTGDIGTMDEDGFF
                     YFKLRLKRMIKSSGMNVYPAQVEDVLYSHPAVSEACVIGVPDEAQVQRVKGFVALKDP
                     AKAGPEMEKELIAHCSEHLIKWSCPREIEFREVLPKTLVGKVAFNVLEQEEIARLKAE
                     GKYAGRC"
     gene            complement(2430..>2651)
                     /locus_tag="P5573_07170"
     CDS             complement(2430..>2651)
                     /locus_tag="P5573_07170"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRT27878.1"
                     /translation="AAQELFGKAGAKEAIAAIEEYFHAIGAPVRLNEVNIDKGEIKKI
                     AENAVSLAKKWGLKSYTEDVIAKILEFAA"
BASE COUNT          460 a          810 c          784 g          597 t
ORIGIN      
        1 gtcgcggtcc atgatcatcg aggccttgac gacggcgtag aggtctgcgc cgcttacgat
       61 gccggggttc tcgcttttga gctcatacag gcgtttcagc gacccacgga tgtcattgaa
      121 ggtacggact gcttttttca ggtcgtcctc gctgatggac gcgcccagtt tgatttccag
      181 gtcgcttctg aatttctcga ggacatctgt catgtatgtc ttcgcgcttt cagtgttgag
      241 cttcacgggc aggacgacgt cgagatggaa atgcccgtgc atgttgaccc gccacacgtc
      301 ggagagcctc tggatggaat cgcaggtgtg ggggaaaacg acgccgtcga ggaaatcgaa
      361 gcggcccgcg agcgcccctt ccagggcgcc ccgcaccagc gagcagcagt aggactggaa
      421 atgggcgtcg gcgaggtgga tgccgtcgcc cgtgccgaaa agacggaagg ggtgcgcccc
      481 cgccgcgtag atcatttctt ccggcgtata ggaacagaag taccccacga ccttccggcc
      541 gctttcctcc ttcagcctct tcccgtatgc agcaggatcc ttcagggcat cgtggaactc
      601 agtcaacatc ttcccgcata tttcccttcc gcctttaacc ttgcgatctc ctcctgctcc
      661 aggacgttga aagcgacctt ccccacgagg gtcttcggca ggacttcgcg gaattcgatc
      721 tcccgcggac aactccactt gatgaggtgc tcggaacagt gagcgatcag ctccttctcc
      781 atttccggtc ccgcttttgc cgggtccttc agggcgacga agcccttcac gcgctgcacc
      841 tgcgcttcgt cgggcacgcc gatgacgcag gcctcgctta cggcggggtg gctgtacagg
      901 acgtcttcca cctgcgccgg atagacgttc attcccgagg acttgatcat ccgcttaagc
      961 ctcagcttga agtagaagaa gccgtcttcg tccatggtgc cgatgtcgcc cgtatggagc
     1021 caaacctttc cgtcggggtg cgtcttcagt gcctgggccg tctcttccgg gttgtccagg
     1081 tacccgagca tcacggctgg cccgtggaga cagatctcgc cttcctctcc gaccggcgct
     1141 tcctccgtcg tgccgagctt cacgaccttc gccgacatgt cggggaaggg gactccgatg
     1201 cctccttccc ggtattcgtc gaagggcgtg accatgatgg ccgtcaccgc ctccgtgagc
     1261 ccgtatccct ccaagagccg gcacgtcccg ccccgctgct tcacgacgga ttcaaaacgt
     1321 tccttgacag tcctcgggag ggtatcggca ccgctgaagg ttgccttgag gcaactgagg
     1381 tcggccttct ggagttccgg gttgcggctc agggcatcga acagtgtggg gaccccgatc
     1441 acgaaggtcg gccgcttctg gcggatgagt ttcgccaccg tccccggcgt gaactgggga
     1501 acaaggatgc tcttcgcacc gcccatgaag caggcgttga cgcagacgcc gagtccgaag
     1561 ccgtggaaga tggggaggat ggcgaggatc gcgtcctttt ccgacagcct tcccacagcc
     1621 gagacctgca tcccctcgct gatgaagttc atgttggaaa gcatgatgcc tttaggcact
     1681 cccgtcgttc ccccgctgta caggatgacg gccatctcgt cggtgtgcat gtccgccctc
     1741 ggcgccggtg ggtgcgctgt tttcattaga tcgctccacc accggaccgc gggatcgtcc
     1801 ggcaccttcg ggatcttccg tcccttcgtg agcatgaaac cgattttttt caggcccgtc
     1861 aggtagtcgg agatgcgcgt gaggatcagc gtctccaggc cggtcttttc tcggagcccg
     1921 gcgaactttc cgtagaaggc gtcgagggtg agggcgaagc ggctccggct ggagttaagg
     1981 tagaactcga cctccttcgg cgtcgaaagg gggtggatca tggccgccac agccccgagc
     2041 ttgttggccg cgtaaaagca gatgatccct tggggcgtcg tgggcatcga tatggcgatc
     2101 cggtctccct ttttcatgcc gagggacgcg agggcgtcgg cgcaccggtc gatctcgttt
     2161 ccgaactgtc ggtaggaggc cgtataaccg agaaaatcgt aggcgacggc ccctggcaga
     2221 cgttccaccg tcttcatgac cgcctcgtac attgtgaccc gcgggtagtc gatggactcg
     2281 gggatgtcgc cgtagaattt cagccagggc ttcttctcgt acatggaccg gaggtctccc
     2341 tttcgggtat cggcgtccgc cgcacagaac ggtgcaggag atcgatgcag gacttgtcga
     2401 ttctcaagca aaagggcgtt tgtatgcccc taagcggcga actctaaaat ctttgcgatc
     2461 acgtcttccg tgtaactctt taatccccat tttttcgcca gggaaacggc gttctcggct
     2521 attttcttga tctccccttt atcgatgttg acttcattca accggaccgg tgcaccgatc
     2581 gcgtgaaagt attcctctat tgccgcaatc gcctctttgg cgccggcttt gccgaacagt
     2641 tcttgcgcgg c
//
LOCUS       DAOTMX010000252         2635 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_30391_length_2635_cov_2.971705, whole genome shotgun sequence.
ACCESSION   DAOTMX010000252 DAOTMX010000000
VERSION     DAOTMX010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30391_length_2635_cov_2.971705"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..1040)
                     /locus_tag="P5573_07175"
     CDS             complement(42..1040)
                     /locus_tag="P5573_07175"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HRT27879.1"
                     /translation="MSTKDHILRYLKENKGNWVSGEFLSLKTNVSRSAVWKHMLRLKE
                     EGYVIESSRKKGYVLLQSSDLLLVSEIQEGLRTRIFGKQEVFHHLETDSTNLRAKILA
                     DQGAPEGTLVVAESQAGGKGRKGRSWFSPAGKGLYASLILRPALPPTEAPRLTLLTAV
                     AAAEALLALAPVPVRIKWPNDITVNGKKLAGILTQASTEVDVLDYVVVGLGVNVNIAE
                     EEFPDDLRDSATSLLIETGATHPRIGMLRLYLEAFEEWYDRFRQSGFLPVLERWKELS
                     DVIGRQVSVHVFNHRHTGEVLDVDPDGALILRDREGVLQKIVSGDIFSPTEVIGKG"
     gene            1247..1372
                     /locus_tag="P5573_07180"
     CDS             1247..1372
                     /locus_tag="P5573_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27880.1"
                     /translation="MGKHKKIERRKELDRRRRRREKRLKMKAKELGAAAAKKPAK"
     gene            complement(1378..1977)
                     /locus_tag="P5573_07185"
     CDS             complement(1378..1977)
                     /locus_tag="P5573_07185"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HRT27881.1"
                     /translation="MKNFWLAFVPMFMAVNAFSILPMFITLTEGLDAPTVRRVIVQSV
                     ITASVTALVFLAVGGFLFDLIGITVADFMIAGGALLFIIALHNLIATTEGPPHLTDTD
                     SLGAVPLGVPLIVGPAVLTTIFLLIGQYGMLITITATVANILIAGVLFWFSGPLIRLL
                     GKAGAKTVSKLGDLILAAFAVMLIRNGIVTVLTASGIAH"
     gene            2140..>2635
                     /locus_tag="P5573_07190"
     CDS             2140..>2635
                     /locus_tag="P5573_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27882.1"
                     /translation="MGIFVILALLPVIPPDARSAQEMSFTRELAEPICEVDTAESARL
                     IAREKVSRMLLDDAVAFLEAETEVRFFGLARGESRALLAGILRVDILKEDKSGGTITV
                     TARLQADTLETVKALDSVRKDRDRVKGFVAVRKKKDEALGEVERFRKEAETAGKDPSR
                     VARYR"
BASE COUNT          577 a          725 c          784 g          549 t
ORIGIN      
        1 atcccgcgaa gcgatgccgt ctgttggccc atttcaaccg cctatccttt cccgatgacc
       61 tcggtcggag aaaaaatatc acccgatact atcttctgta agacaccttc cctgtcccgg
      121 aggatcagcg ccccgtccgg gtcgacgtcc aggacctctc cggtgtgccg gtggttgaag
      181 acatggacac tgacctggcg gccgatgacg tccgaaagct ctttccaccg ttcgaggacg
      241 ggcaaaaagc ctgactgcct gaatcggtcg taccactcct cgaaggcctc taagtaaagc
      301 ctaagcatgc ctatcctcgg gtgggtcgcc cccgtctcga tcaaaagcga tgtcgcgctg
      361 tcccggaggt cgtcgggaaa ttcctcctcc gctatgttga cgtttacccc cagtccgaca
      421 actacgtaat cgaggacatc gacctccgtg cttgcctggg tgaggattcc cgccagcttt
      481 ttgccgttta cggtgatgtc gttcggccac ttgatgcgca ctgggacggg tgccagggcg
      541 agcagggcct ccgcggcggc gacggcggtc aggagggtca acctgggtgc ctcggtcggc
      601 ggcagggccg gcctcaggat caaggaggcg tataaaccct tcccggcagg tgaaaaccac
      661 gacctccctt tcctcccctt tccgcccgcc tgactttcgg caacgaccag ggttccttcc
      721 ggtgcgccct gatcggccag tatctttgcc cgcaggtttg tcgaatcggt ctcgagatga
      781 tgaaagacct cctgtttgcc gaagatgcgc gtcctcaacc cttcctggat ttcgctgacc
      841 aggaggaggt ccgaagattg taggagaaca tatcctttct tccttgacga ctcgatgacg
      901 taaccctcct ccttcagccg aagcatatgt ttccatacgg ctgaacggga gacattcgtc
      961 ttcaggctga gaaattcccc ggaaacccag ttccctttgt tttccttgag gtatctcagg
     1021 atatggtctt tcgttgacat gacggggttt caatatacca acctcgtttt tttgtcaacc
     1081 cggtttatat gaagggttga taaacggcgg gctaccttcg ttcacccctc cagcgcgccg
     1141 acattacatt gacacattac attgatattg cttgcaaggc ggggcgattt ttcttactat
     1201 tacgcccgat cgatcgcgat tcatgactct atttacggag gtgtctgtgg gaaaacacaa
     1261 gaagatcgaa cgccggaaag aactggaccg ccgaaggcga aggcgggaaa agcggctcaa
     1321 gatgaaggcc aaggagttag gggccgcggc ggcgaaaaag cctgcgaagt aaagagatta
     1381 atgggcaatc ccggatgcgg tcagaactgt gacgatcccg tttcggatca acatgacggc
     1441 gaaggcggcg aggatcaggt ctccaagctt ggagaccgtc ttcgcaccgg cctttccaag
     1501 taggcggatg aggggcccgg aaaaccagaa cagcacgccg gcgatgagga tgttcgccac
     1561 ggtggccgtt atcgttatga gcatcccgta ctgaccgata aggaggaaaa tcgtcgtcag
     1621 aacggccggc ccgacgatca gcggcactcc cagcggcacg gcgccgaggc tgtcggtgtc
     1681 ggtaaggtgc ggcggccctt cggtagtggc gatgaggttg tggagggcga tgataaaaag
     1741 cagcgcccca ccggcgatca tgaagtccgc gaccgttatc ccgatcaggt cgaaaaggaa
     1801 acccccgacg gcgaggaata caagcgccgt aaccgatgcc gtgatgacgg actgaacgat
     1861 gacccgccgg accgtggggg cgtcgaggcc ttctgtcaag gtgatgaaca tgggcaggat
     1921 gctgaaggca ttcactgcca tgaacatcgg gacgaaggca agccagaagt tcttcatagg
     1981 ggcctctcac cttgtttgtc attctacact tccgggcggg gatacggcaa ttcaaaaaaa
     2041 tcaaccgata tctgcgggaa gagattcttc tttgcgtctg tgacattaag gccctgggga
     2101 aacagtttgg cgaatctttc gagaaaaatt tttgcactta tgggcatctt tgtgatcctg
     2161 gcccttctcc cggttatccc tcccgacgcc cgcagtgctc aggagatgag cttcacacgg
     2221 gagctggcgg agcctatttg tgaggttgat acagcggagt cggcacgcct gatcgcccgc
     2281 gagaaggtca gtcggatgct cctagacgac gcggtagcct tcctcgaggc agagacggaa
     2341 gtgagattct tcggcctggc gaggggagaa tcgagggcac tccttgcggg aatccttcgt
     2401 gtcgacatac tcaaggaaga taagagtggg ggaacgataa ctgttacggc ccgcctccag
     2461 gccgacacac tggaaacggt caaggccctc gactctgtgc gtaaggacag ggaccgcgtg
     2521 aagggcttcg tcgcggtcag gaaaaagaaa gatgaggccc tcggtgaggt cgagcggttc
     2581 aggaaggagg cggaaacggc ggggaaagac ccttcccggg ttgcccggta tcggg
//
LOCUS       DAOTMX010000253         2618 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_30661_length_2618_cov_2.875146, whole genome shotgun sequence.
ACCESSION   DAOTMX010000253 DAOTMX010000000
VERSION     DAOTMX010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30661_length_2618_cov_2.875146"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="P5573_07195"
     CDS             <1..214
                     /locus_tag="P5573_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27883.1"
                     /translation="LFLQLTARDRHDVGIPEDDLSPGVITFGVLKEAQALGDRRVLEA
                     ARRRVLRCDFGSDISEGLKRLLSAVK"
     gene            286..978
                     /gene="rpiA"
                     /locus_tag="P5573_07200"
     CDS             286..978
                     /gene="rpiA"
                     /locus_tag="P5573_07200"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF001924.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HRT27884.1"
                     /translation="MKPDTAELKRQAAAFAVELVESGMVVGLGHGSTAIIAIGLIAEK
                     MASGALKDVTGIPCSLQAEEEARRFKIPVASLEDYPSLDITIDGADEVDPHLNLIKGG
                     GGALLREKIVAQASRREVIVVDESKVVNNLGEKRPVPVEVIPFGWHSQLDYLKSLGAR
                     VMARRNSNGSFFTTDQGNLILDCSFGPIHDPYELACRIKERAGVVEHGIFLGLATDVI
                     VAGAEGIRHLKP"
     gene            1009..1440
                     /locus_tag="P5573_07205"
     CDS             1009..1440
                     /locus_tag="P5573_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27885.1"
                     /translation="MVSRKTGFSVFGFALYIFFIMSLPALAHAPRELDLAYDFPNQTL
                     TVTIEHWAVSPTVHFVEKVEVFKNGKKVESREYQSQPDSERFTYIYKIEAMEGDVFNV
                     VAKCSVFGTTTAKLTVKKLQEIPVITAPPEEKKAAPADGGR"
     gene            1504..2049
                     /locus_tag="P5573_07210"
     CDS             1504..2049
                     /locus_tag="P5573_07210"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HRT27886.1"
                     /translation="MAIPDVEKSCLIVVDVQDRLYRAMHEKEALAENIQKLIKGVRLL
                     GVPVIATEQYPQGIGSTIEPVAALLAGVPIVPKLHFSCCGDAVFLKTFKDLNRKQVLI
                     AGIECHVCVYQTAVDLLDRGHEVHVVADAVSSRTTRNLEIGLQGMRDEGAKIAGTETV
                     LFALLKTAGAEKFPEVLRIVK"
     gene            2059..>2618
                     /locus_tag="P5573_07215"
     CDS             2059..>2618
                     /locus_tag="P5573_07215"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRT27887.1"
                     /translation="MKPIYLDHNATTPVDPEVFDAMRPYLTERFGNPSSPHVYGVEAR
                     RAVEKARTRVASLLDCEPEEVVFTSGGTEANNHAIKGACLARRDKGRHIVTSAVEHPA
                     VIEVCRYLETMGFTLTVVPVDAYGVVDLPALKKALTPETTLISVMHANNEVGTLQPVE
                     EIAGIARERGIIFHTDAAQTAGKIPVS"
BASE COUNT          608 a          744 c          799 g          467 t
ORIGIN      
        1 gctcttcctg cagctaaccg cccgcgacag acatgacgtc ggcatcccgg aggacgacct
       61 ctctccggga gtaataacct tcggcgtcct caaggaagcc caggccctgg gggatcgcag
      121 ggtgctcgag gcggcgcgga ggagggtcct caggtgcgac ttcggttccg acatctcgga
      181 gggcctgaag cgtttactct ctgcggtgaa gtgaagaggc tgcggaatga ggaaacggcg
      241 gtttattacc ccacaaatct cctatcgcga aacaggagga gaaccatgaa accggacaca
      301 gcggaactga aaaggcaggc ggcggccttc gccgttgaac tggtcgaatc ggggatggtc
      361 gtcggcctgg gtcacggcag caccgccata atcgccatag gcctcatcgc agaaaaaatg
      421 gcgtcggggg cgctcaaaga cgtgaccggc ataccctgct cgctacaggc cgaagaggag
      481 gcccgtagat tcaagatacc tgtggccagc ctcgaagact atccctccct cgacatcacg
      541 atagacgggg ccgacgaagt ggacccccat ctaaacctca tcaagggagg cggcggggcg
      601 ctcctgcgtg agaaaatcgt cgcccaggca agccgccggg aggtcattgt ggtcgatgaa
      661 tccaaggtgg tgaacaactt gggtgaaaaa aggccggtgc cggtggaggt catccccttc
      721 ggttggcatt cccagttgga ctacctgaaa tccctcggtg ctcgcgtcat ggcgcggaga
      781 aacagtaacg gcagtttctt caccacggac cagggaaacc tgatcctaga ttgcagtttc
      841 ggcccgatac acgaccccta tgaattggcc tgtcggataa aggagcgggc cggcgtcgta
      901 gaacacggca ttttcttagg cctggcgacg gacgttatcg tcgccggggc cgagggcata
      961 agacatttga agccgtaagt cgagatgttc cacgaggaga caagagctat ggtatcaagg
     1021 aaaacaggtt tttcggtttt tgggtttgcg ctgtatattt tctttatcat gtcgctgccc
     1081 gccctggcgc acgccccccg ggaattggac ctggcgtatg atttccccaa tcaaaccctg
     1141 accgtgacga tcgaacactg ggccgtatcc ccgactgtcc atttcgtcga gaaggtggaa
     1201 gtattcaaga acgggaagaa agtcgagtcc agagaatacc agagtcagcc ggactcggaa
     1261 agattcacct acatctataa aatcgaggcc atggagggtg acgtctttaa tgtcgtggcg
     1321 aaatgcagcg tcttcggcac cacgacggcc aaattgacgg tcaaaaaact gcaagagatc
     1381 cctgtcatca cggcaccgcc ggaagaaaag aaggcagcgc ctgcggatgg gggcagataa
     1441 agaagtcgag tagtcgagac cgaaaaatct tgcgatggtc ggtcaccact tgagggggaa
     1501 gcaatggcga taccggatgt agaaaagagt tgcctgatcg tagtcgacgt tcaggacagg
     1561 ctgtaccggg cgatgcacga gaaggaggcc ctggcggaga acatccaaaa gctgatcaag
     1621 ggcgtccgcc tcctcggcgt accggtcatc gccaccgagc agtacccgca gggaatagga
     1681 tcgaccatcg agccggtggc tgctttgctg gccggcgtcc ccatcgtccc gaagctccat
     1741 ttcagctgtt gcggcgacgc ggtattcttg aagaccttca aggacctcaa caggaaacag
     1801 gtcctgatcg ctggcatcga atgtcacgtc tgtgtatacc agacggctgt ggacctgctc
     1861 gaccgcgggc atgaggtcca cgtcgtcgcc gatgccgtat cgtcgaggac gacccggaac
     1921 ctggaaatcg gcctccaggg gatgcgcgat gagggcgcga agatcgcggg cacggagacc
     1981 gtcctcttcg cgctcctgaa aaccgccggg gcggaaaagt tccccgaggt gttgcggatc
     2041 gtcaagtagt cccgaacgat gaagcccata tatcttgatc acaatgccac aacccccgtc
     2101 gaccccgagg tcttcgatgc catgaggcct tacctgacgg agcgcttcgg caatccctcg
     2161 agcccccacg tctacggagt agaggcccgg agggccgtcg agaaggccag gactcgggta
     2221 gccagccttc tcgattgcga acccgaagaa gtggtcttca cgagcggagg gacggaagca
     2281 aacaaccatg ccataaaagg tgcctgcctc gcccgtcgcg ataaaggcag gcacatcgtg
     2341 acgtcggccg tggagcaccc ggcggtgata gaggtctgcc ggtacctcga gacgatgggg
     2401 ttcacgctca ctgtcgttcc ggtcgacgcg tacggcgtgg tggacctacc tgccctaaaa
     2461 aaagcgctga ccccggagac gaccctcatc tccgtcatgc acgccaataa cgaggtaggg
     2521 accctccagc ccgtcgaaga aatagccggc atcgcccgtg agcgggggat aatattccac
     2581 accgatgccg cccagacggc gggcaagatc cccgtctc
//
LOCUS       DAOTMX010000254         2612 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_30753_length_2612_cov_3.162691, whole genome shotgun sequence.
ACCESSION   DAOTMX010000254 DAOTMX010000000
VERSION     DAOTMX010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30753_length_2612_cov_3.162691"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="P5573_07220"
     CDS             complement(<1..547)
                     /locus_tag="P5573_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HRT27888.1"
                     /translation="MFESLTDKLDQVFRRLKGRGRLDEENVRQALKDIRMALLEADVN
                     FKVVKEFTEDVRQRTVGQDVLESITPGQQVVKIVHDRLVELMGGGSSHLRLANRFPAP
                     VMLVGLQGCGKTTTAAKLAYYLKNEERRVFLVSADVYRPAAVEQLRVLADKVGAGFFT
                     STDGMDAVRICTEAVKEAEKGG"
     gene            complement(631..1260)
                     /locus_tag="P5573_07225"
     CDS             complement(631..1260)
                     /locus_tag="P5573_07225"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRT27889.1"
                     /translation="MEIRRPRKFELRLGKVGFVLFTFGVSCLLLIAFVLGVMVGKNIE
                     TYPEKITGAIPGFVREKIAAVPVSPQIAVGPEDVKEAVKEDGDVKLTFYDNLSARKEP
                     GDGRPGDVAAEKPETAKGAAQKERPEAHENYVLRVASLKDEEKAKELQKKLSLMGYKS
                     SVEVRELKSGTFFRVIVVGLGALDEAEKAADRVQKETKAKCVVAKETGP"
     gene            complement(1268..>2612)
                     /gene="argS"
                     /locus_tag="P5573_07230"
     CDS             complement(1268..>2612)
                     /gene="argS"
                     /locus_tag="P5573_07230"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HRT27890.1"
                     /translation="GDRYGESSVGGGKRVQVEFVSANPTGPLHIGHARGAVIGDVIAN
                     TLRAVGCDVTKEYYINDVGNQMNTLGLSVYLRYRELLGEKVDFPPNYYQGKYIQDLAA
                     QVITIQGDKFLNADEKDILSFFVPYAAGAIMDGIREDLRSFGVVFDNYFSEQELYRDD
                     GVARLIEELQEKGFIYREGNTLWFRTTDFGDEKDRVVVRENGEPTYFAADIAYHRNKY
                     ERGFEEIIDVWGADHHGYLPRMYAAIQALGREKDTLKVVLVQLVNLLRGGKPVAMSTR
                     SGEFVTLREVVDEVGKDAARYNFLMRRSDSHLDFDLEIAKKQSNENPVYYVQYAHARI
                     CSILKNAHERRYSVPRYGEIEPQLLKLPEEIMLIKYISRYPEVVEGSAQALEPHRYTF
                     YLNELAAVFHSYYNKHRVVGEDAALSRARLFLVNAVGVVLRNALRILGVTAPEKM"
BASE COUNT          513 a          841 c          637 g          621 t
ORIGIN      
        1 agcctccctt ctcggcttct ttcacggcct cggtacaaat ccgcacggcg tccatcccgt
       61 cggtcgaggt aaagaaaccc gccccgacct tatcggccag caccctcaac tgttctaccg
      121 ccgccggtcg gtatacatcg gccgaaacaa ggaacaccct tctttcctca ttcttcaagt
      181 aatacgcgag cttggctgcg gtcgtcgttt ttccgcatcc ctggagccca accagcatga
      241 ccggcgccgg aaaacgattt gcaagcctga gatggctgct cccgccgccc atcaactcga
      301 cgagacggtc atggactatc ttgaccacct gctggcccgg cgttatgctc tccaggacgt
      361 cctgtccgac cgtccgttgt ctcacgtcct ccgtgaattc cttcaccacc ttgaagttga
      421 catccgcctc caaaagggcc attcggatgt ctttcaacgc ctggcggacg ttctcctcgt
      481 ccagtcggcc gcggcccttg agccgcctga aaacctggtc cagtttatcg gtgagacttt
      541 cgaacataac gaaaaacctt tagatgtcag cttccggcat taagattgac ctcttcggct
      601 tgtcagcctc taaactcctt gcttaaccgg ctatgggcct gtctccttgg cgactacgca
      661 cttcgccttc gtttcctttt ggacgcggtc cgccgccttc tcggcctcgt ccagtgcccc
      721 tagaccgact acgatgacgc ggaaaaaagt gcccgacttc agttccctca cttccaccga
      781 cgacttgtac cccataaggg agagcttctt ttgcagttcc tttgccttct cctcgtcttt
      841 cagtgaggcc acccgcaaga cataattttc gtgggcctcc ggtctttcct tctgcgcagc
      901 acccttcgcc gtctccggct tctccgccgc gacatcccct gggcgaccgt ctccgggctc
      961 cttcctcgct gagagattat cgtagaacgt cagtttcacg tccccgtctt cttttacagc
     1021 ctctttcacg tcctccggcc cgaccgcgat ctgcggtgag acaggcacgg ctgcgatctt
     1081 ttcccgcaca aagccaggga tagcccctgt gatcttctcc gggtacgtct cgatattttt
     1141 accgaccatc accccgagga cgaaggcgat cagcaagagg caggagaccc cgaaagtaaa
     1201 gagaacgaaa ccgaccttgc ccagtctgag ttcgaacttt ctcggtctcc ggatttccat
     1261 gggtgcatca catcttctcg ggcgccgtta cacctaatat cctcaaggca ttcctcagaa
     1321 caacccccac ggcattcacc aagaacagcc ttgccctgct caatgccgcg tcctcgccga
     1381 cgacacggtg tttattatag taactgtgga agaccgccgc aagttcattc aagtaaaagg
     1441 tatagcggtg cggctcgagc gcctgggcgc ttccttccac tacttcagga taccggctga
     1501 tgtattttat gagcataatc tcctccggca gcttgagcag ctgaggctct atctcgccgt
     1561 accgggggac cgaataacga cgctcatggg cgttcttcaa gatgctgcat atccgggcat
     1621 gggcgtactg gacataataa accgggtttt cgttcgattg cttttttgca atctccaggt
     1681 cgaagtccag gtggctgtcg gaccggcgca tgagaaaatt gtacctcgcc gcgtctttcc
     1741 cgacttcgtc tactacctcc ctcaaggtca cgaactcgcc ggaacgggtc gacatcgcca
     1801 ccggctttcc gccgcgcagg agattgacga gctggacgag gacgaccttc aacgtatctt
     1861 tctccctgcc cagggcctgg atggccgcat acatcctggg tagataaccg tggtggtcag
     1921 ccccccagac atcgatgatc tcttcgaaac cccgctcata cttgttgcgg tggtaagcga
     1981 tatcggcggc gaagtaagta ggttccccgt tttccctgac gacgacacgg tccttttcat
     2041 caccgaaatc ggtcgtccgg aaccagaggg tgttgccctc cctgtagatg aagccctttt
     2101 cctgcagctc ctcgatcagc ctggcgacgc cgtcgtcccg gtagagctcc tgttcgctga
     2161 agtagttgtc gaaaaccacc ccgaaggagc gcaggtcctc tcggatgccg tccattatcg
     2221 ccccggcggc ataggggaca aagaaagaaa ggatgtcttt ctcgtcggcg ttcagaaatt
     2281 tgtctccctg tattgttatt acttgagcag cgagatcctg aatgtacttt ccctggtagt
     2341 aattaggcgg aaaatcgact ttttccccca acaactcccg gtaacgcaga tagacggata
     2401 gaccgagcgt gttcatctgg ttgccgacgt cgttgatata gtactctttt gtcacgtcgc
     2461 accctaccgc cctgagggtg ttggctatca cgtcaccgat cacggcgccc cgggcgtgcc
     2521 cgatatggag aggacccgta gggttggcac tcacaaactc cacctggacc cgcttccccc
     2581 caccgacaga agactcgccg tagcggtccc ct
//
LOCUS       DAOTMX010000255         2607 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_30834_length_2607_cov_3.572100, whole genome shotgun sequence.
ACCESSION   DAOTMX010000255 DAOTMX010000000
VERSION     DAOTMX010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30834_length_2607_cov_3.572100"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            229..420
                     /gene="rpsU"
                     /locus_tag="P5573_07235"
     CDS             229..420
                     /gene="rpsU"
                     /locus_tag="P5573_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HRT27891.1"
                     /translation="MEVKVINNDIDKALKVLKNKLSKSGLFKELKLRRAYEKPSVRKK
                     RKAIEARRRLAKAQRRRSS"
     gene            504..1157
                     /locus_tag="P5573_07240"
     CDS             504..1157
                     /locus_tag="P5573_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HRT27892.1"
                     /translation="MREKQYVVDALSIEESWRIFRIMAEFVEAIEELSQLGYAVSIFG
                     SSRVTPEDEYYKKAVYLARRLVEKGFAVITGGGPGIMEAANRGAAEGGGKSVGMNIRL
                     PFEQKPNPYANIRIEYKYFFVRKVMFVKYAMAYVVMPGGFGTMDELFEAVTLIQTKRI
                     KAFPVILMGSDYWNGLLDWVRATMLSERKIQPEDMELLQVMDDPDQVVKHIQKLVIV"
     gene            1176..2588
                     /gene="holA"
                     /locus_tag="P5573_07245"
     CDS             1176..2588
                     /gene="holA"
                     /locus_tag="P5573_07245"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HRT27893.1"
                     /translation="MTKKMSLEPSLKSVLADIRKGKVAPCYLLHGDEDYLVGTALHEI
                     VGALIPEADREMNLSYLEGEKEDALSLCRVLLTPPLIPGRKAVVVKNSRLLSSRQSLP
                     DMVRKIKETLESDPTRAARIFMAFVRTAGWDIDDLRGENWRYIPAEEWRRLTGEEADE
                     REKWLPVLVEMCSQSGVSAEPETGGEEEINRVLQEGLPDGSCLVITAEVVDRRKKLFK
                     TISEKGVVLNFQQAKGRAGQKSRLFEEAKEFLRERGMDIEPKALNALATKTGADLRTT
                     MAELEKLIVYGEGKSSITEREVEEAVEKTGEASLFAFTTFLVEKNLSGAVSVVRELLG
                     QGIHPLAIVAMVVREYRLLLHAKALVMSGKIQDFDRTMDYGRFQKAVQPLLKELSSAD
                     GEGEPLELLLQHPYVIYNALRSSGNYSLDELIGVLDCLTHVDLTLKSSGQDTQLILER
                     LAVRICDPSLSPAPRPSPHL"
BASE COUNT          645 a          634 c          752 g          576 t
ORIGIN      
        1 gaggccttga gggacaagct cctgacggac aaatacctta gtgggcaatg gaacagggag
       61 ggaagggtgc cggcgaaaga ccggatttcc tgagtattgg ggggatattt tttggccggc
      121 agcctgatga gttttctatt gacaaatcat atacttttat atatagcttt tatactttac
      181 taaaaaggaa ttttgtcatt caaattatca tagaaagggt gtggagtttt ggaggtaaag
      241 gttattaaca atgatatcga taaggccttg aaggttctga agaataaact ctccaagagc
      301 ggcttattta aagaactgaa actgcgtagg gcctatgaaa agccttcggt acgaaaaaag
      361 agaaaggcaa tcgaagcccg cagaaggctt gctaaggcgc aaagaaggag gtccagctga
      421 caaccctttt gagcatcata cgtgcgtttt tcgaaagcag ggacacttga caaagtgtcc
      481 ctgctttgtt taaggtgttt cctatgaggg aaaaacagta tgtagtcgat gccctgtcga
      541 tagaagagtc ctggcgcata ttccgcatca tggcggagtt tgtcgaggcc atcgaagaac
      601 tttcgcagct tgggtatgcg gtcagcatct tcgggtcctc ccgggttaca ccggaagacg
      661 aatactataa gaaggccgtt taccttgccc gacgcctggt ggaaaagggc tttgccgtca
      721 tcacgggggg cgggcccggt atcatggagg cggccaatcg gggtgccgct gagggagggg
      781 ggaaatcggt cgggatgaac atccgtctgc ccttcgagca gaaacccaac ccttacgcaa
      841 acatcaggat agaatacaaa tactttttcg tccgcaaggt gatgttcgtg aagtatgcca
      901 tggcctacgt cgtcatgccg gggggcttcg gcacgatgga cgagcttttc gaggcggtta
      961 ccctcatcca gaccaagcgg atcaaggcct tcccggtgat tctgatgggc agcgattatt
     1021 ggaatggtct cttggactgg gtaagagcta ccatgctcag cgagagaaag attcaaccgg
     1081 aagacatgga actcctccag gtcatggatg accccgatca ggtagtgaaa catattcaga
     1141 aactcgtcat cgtttgataa acccctaccc aacctttgac aaagaaaatg agcctggaac
     1201 ccagccttaa atccgtgctg gccgatatca ggaagggcaa ggttgcacca tgttatcttc
     1261 ttcacggtga cgaagattat ctggtcggca ccgccctgca cgagatcgtc ggcgccctta
     1321 tacccgaagc agacagggaa atgaacctat cctacctgga gggagaaaaa gaagatgccc
     1381 tcagcctctg ccgggtcctt cttacgcctc ccctgatccc gggaaggaag gcggtcgtcg
     1441 taaaaaactc gcgcctcctt tcgtcccgcc aatccttgcc cgacatggtc cgcaaaatca
     1501 aggagacctt ggagagtgac cctacccgtg cggccaggat tttcatggcc ttcgtaagga
     1561 cggccggctg ggacatcgat gatttgcggg gcgaaaactg gagatacatc cccgcggaag
     1621 agtggaggcg actgaccggc gaagaggccg acgaacggga gaaatggctt cccgtgctgg
     1681 tcgagatgtg ctcgcaatcg ggggtgtctg ccgagccgga gacgggcggg gaggaggaaa
     1741 tcaacagggt cttgcaggag ggacttccgg acggcagttg tcttgtgatc acggccgagg
     1801 tcgtagacag gagaaagaaa ttattcaaga ccatctccga gaagggcgtc gttctcaact
     1861 ttcagcaggc aaagggcagg gcgggacaga agagtcgttt attcgaagag gcaaaggagt
     1921 ttctccggga acggggaatg gacatcgagc caaaggcgct taacgcgctc gccacgaaaa
     1981 cgggtgccga ccttcggaca acgatggcgg aactagaaaa gctgattgtc tacggagagg
     2041 ggaaatcgtc gataacggaa agggaggtgg aagaggcggt agaaaaaaca ggggaggcct
     2101 ctctattcgc ttttacgact ttcttggtcg aaaagaatct ttccggggct gtttcagtcg
     2161 tccgcgagct tctgggtcag ggcatccatc cgctggcgat cgtcgccatg gtcgtcaggg
     2221 aatacaggct ccttctccat gcgaaggccc tcgtcatgtc gggaaagata caggatttcg
     2281 accgcacaat ggactacggc cggtttcaga aagccgtcca gccgctcttg aaagaactgt
     2341 cttccgccga cggggagggg gaacctctgg agcttctcct tcagcaccct tacgttattt
     2401 acaacgccct caggtcttca ggaaattact cgctcgatga gctgatcggc gtcctcgatt
     2461 gtttgaccca tgttgacctg acccttaagt cttcggggca ggatacccag cttatcctgg
     2521 agcgcctggc tgtacggata tgcgatccct ctttgtctcc ggcccctcgc ccttcgcccc
     2581 acctataact ttttgatctt cgttgct
//
LOCUS       DAOTMX010000256         2604 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_30886_length_2604_cov_3.770891, whole genome shotgun sequence.
ACCESSION   DAOTMX010000256 DAOTMX010000000
VERSION     DAOTMX010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30886_length_2604_cov_3.770891"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            37..1533
                     /locus_tag="P5573_07250"
     CDS             37..1533
                     /locus_tag="P5573_07250"
                     /inference="COORDINATES: protein motif:HMM:NF023222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HRT27894.1"
                     /translation="MMNGDEYIRSLQAMKTVVYVNGQKVSDYYNHPVMKPAVNTLAAI
                     YDLASDPELDRDIHALIVTESDLTGGRVNRFLKILQSQEDLLARMKLQRALMRYTGGC
                     FGGRCVAGAVINSLWSVTYEMEKAGCSAPYHQRFRNYMKAVQEQDLAVSGNITDAKGD
                     RSLPPHRQSDPDLYVRVVERRKDGIVVNGAKLQQSGAPVAHERLVVPTTALGPDDEPY
                     AVAFAVPGDAEGVVHVNDSACINAKFEAMDHEDIGNATFGMHQSAHIFFDNVFVPWER
                     VFLCGEWEASRDLVHRFSDFQRLASSSCRCGYIDLCIGAALAMADYNGVASKSHIQEK
                     LIDMSVDAETIYGLVAGASAHSTDTASGVPLPDTLIINAAKLYMNDAILRTAQKLAEI
                     GGGILVTRPSNRDLRIPKVGDLLRKYHKGRDGIDVGDRVKMARLCESLSGLASMTPIL
                     SVIAAGPPATQRLNFRLMTDLKRDRRAAERLAGIAPKKKHKNGKEFSR"
     gene            1530..2453
                     /locus_tag="P5573_07255"
     CDS             1530..2453
                     /locus_tag="P5573_07255"
                     /inference="COORDINATES: protein motif:HMM:NF017652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HRT27895.1"
                     /translation="MKEKAIITAAITGSIHTPGMSEYLPVTPQEIADDAVRAYEAGAA
                     VCHIHARDPQTGQPTPDVNVFREAITRIKSKCDMVVCITTGGGLGMTAEQRLVAVSTF
                     KPELASFNAGSVNFALFHALERYKNFKFDWESKYLAMTEDYIFPNTFKSMRDFARIFA
                     ENGTKPEFEVYDSGMVNNIAFMLERGYVKKPVYIQFVMGVLGGITPSARNLLFLVDYA
                     RELIGDFEFSVCVAGRAQFPICTHSLLIGGNARVGLEDNLYLEKGRMAKSNAEQVAKI
                     VRIAREFGIEPATSNEARKILGLKGIDKVNF"
BASE COUNT          599 a          739 c          730 g          536 t
ORIGIN      
        1 ccctctgaca agaggatgaa agggagatgt tcagttatga tgaacggtga cgaatacatc
       61 cggtccctac aggcgatgaa aaccgttgtt tacgtaaacg gccaaaaagt cagcgactat
      121 tacaaccatc cggttatgaa accggccgtc aataccctgg cagctatcta tgaccttgcg
      181 tccgaccccg aacttgatcg tgatatccac gctcttatcg ttacggaaag cgaccttacc
      241 ggcggcaggg tcaaccggtt tctcaagatc cttcagtcac aggaagacct cctggccagg
      301 atgaagctgc agcgcgccct gatgcggtac actggtgggt gtttcggggg acgatgcgtt
      361 gccggtgccg tcatcaactc cctgtggtcg gtcacctacg agatggaaaa ggccgggtgc
      421 tccgctccgt accatcaacg attcagaaac tacatgaagg cggttcagga acaggacctg
      481 gctgtttccg ggaacatcac ggatgccaaa ggcgaccgct ccctgccgcc ccatcgacag
      541 tccgaccccg acctctacgt cagggtcgtc gagaggagga aggacggcat cgtcgtcaac
      601 ggcgcaaaac tccagcaatc cggggcccct gttgcccatg aaaggctcgt cgttccgacg
      661 acggcactgg ggcccgatga tgaaccctat gcggtggcct ttgccgtccc cggtgatgcc
      721 gagggtgtcg ttcatgttaa cgattccgcc tgtatcaacg caaagttcga ggccatggac
      781 cacgaagaca ttggcaacgc gacgtttggg atgcatcaaa gcgctcacat ctttttcgac
      841 aacgtcttcg ttccctggga aagagtcttc ctgtgcggag aatgggaagc gagccgcgac
      901 cttgtccacc gtttctccga ttttcagcgt ctggcctcct cctcctgccg gtgcggctac
      961 atagatcttt gcatcggtgc cgcgttggcc atggctgatt acaacggcgt tgcgtcaaaa
     1021 tcacacatcc aggaaaaact catcgacatg agcgtcgacg cggaaactat ttacggtctt
     1081 gttgccggtg catcggcaca ttctaccgac acggcctccg gcgtgccgct ccccgacacc
     1141 ctgatcatca acgccgccaa gctttacatg aacgatgcaa tcctgcgaac cgcacagaaa
     1201 ctcgctgaaa taggaggggg aatcctcgta accaggccga gcaaccgaga cctaaggata
     1261 ccaaaggtcg gcgaccttct ccggaaatat cacaaaggcc gggacggcat cgatgtcggg
     1321 gatagggtga agatggctcg gctttgtgaa tcactttcag gtcttgcctc tatgacgccg
     1381 attctttccg tcatcgcggc aggacctccg gcaactcagc gtttgaactt tcggctcatg
     1441 acagacctga aacgcgaccg gagagcagcc gaaagacttg ccggtatcgc acctaaaaag
     1501 aaacacaaaa acggaaagga gttcagcaga tgaaagaaaa agcaatcata accgcggcca
     1561 tcacgggcag cattcacacg ccaggcatgt ccgagtatct accggttacc ccccaggaga
     1621 ttgccgacga tgccgtccgt gcctatgagg cgggggctgc cgtttgccac atccatgccc
     1681 gagatcccca gacagggcag ccgaccccgg atgtgaacgt gttcagggag gccatcaccc
     1741 ggatcaagag caagtgtgac atggtggtct gcatcacgac cgggggtggc ctcgggatga
     1801 cggcggaaca gcgtctcgtc gccgtgtcta ctttcaagcc ggagttggcc tccttcaatg
     1861 ccggttcggt caacttcgcc cttttccatg ccctggagag gtacaagaat ttcaagttcg
     1921 actgggagag caagtacctg gccatgaccg aggactacat cttcccgaac accttcaagt
     1981 ccatgaggga ttttgcccgc atcttcgccg agaacggcac caagccggag ttcgaggtct
     2041 acgattcggg gatggtcaac aacatcgcct tcatgcttga gaggggctat gtgaagaagc
     2101 cggtgtacat tcagttcgtg atgggtgtcc tgggcgggat aaccccgagt gccaggaatc
     2161 ttctcttcct cgtggattat gcccgggaat tgatcggtga cttcgagttt tccgtgtgcg
     2221 tggcggggcg agcacagttc cccatctgca cgcattcgct tctcatcggc ggaaacgcgc
     2281 gcgtcggcct ggaggacaac ctgtacctcg aaaagggcag gatggctaag agcaacgccg
     2341 agcaggtggc gaagatcgtc cgtatcgcac gtgagttcgg catcgaaccg gcgacgtcga
     2401 acgaggcgag gaagatactg ggactgaaag gaatcgacaa ggtgaatttt taaagtttct
     2461 tatgcccggt ctcggttcaa gttaaaccct cgaataaaag ggccggtctc accggccctt
     2521 ttattcgtcg tgcgcccgtg ccgggcgcac gaaaatacgc cgggactctt caatgtttga
     2581 tagctgctca aatgaatctc gcct
//
LOCUS       DAOTMX010000257         2596 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_31005_length_2596_cov_4.083432, whole genome shotgun sequence.
ACCESSION   DAOTMX010000257 DAOTMX010000000
VERSION     DAOTMX010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31005_length_2596_cov_4.083432"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..478)
                     /locus_tag="P5573_07260"
     CDS             complement(110..478)
                     /locus_tag="P5573_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27896.1"
                     /translation="MTTPEGTPQKVRINQQNLYKEEVFTDIRVAAIRQLTPVKTNGEV
                     DKSRKIIFFGQTQLVSPHGPLPIQFPIEAKNLHEAIEKFPETMQSFMEKMIEEAKEMQ
                     RQEQSRIIVPGASESGVILK"
     gene            676..1143
                     /locus_tag="P5573_07265"
     CDS             676..1143
                     /locus_tag="P5573_07265"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRT27897.1"
                     /translation="MSRKKIFPFPAERFDTFESVLKIDTTSQRHLLHVGDEVEQVWAA
                     WALGLKLGAHITPELVECLKTCRFPGTRRHFLVVLAGLGERETLEKYAVNDPAPLVRA
                     TACRYLARTAEPGNMRIGRFLLERLREDASPEVREAILEELDRGKNGTGSGQE"
     gene            complement(1156..2052)
                     /locus_tag="P5573_07270"
     CDS             complement(1156..2052)
                     /locus_tag="P5573_07270"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRT27898.1"
                     /translation="MRFRKAGKVADNLWYLGREEAGVYVLEGKASSILINGGLSYILP
                     DVLRQMKEFGIDAEKIDKILILHSHFDHVGIIPYFKRAYPAIEVCASEPAWKTLAMPK
                     AIEVINSFSRLSAKHVGLESELAPYDIDWRDDIKGKTLAEGDRIELGGAFLEIIYTPG
                     HTNCSISAYEPNLKAMFASDAGGIPYRDTSFPSMNTNMDQYLQSLEKLKVYPVAYLCA
                     DHYGYITGEEAGGFIDLTLREGRRWKAYMEDVYRKFNGDIDAASKATNEYFYKEMPDY
                     FIAPDILESVFRGMYKYISKNI"
     gene            complement(2174..>2596)
                     /locus_tag="P5573_07275"
     CDS             complement(2174..>2596)
                     /locus_tag="P5573_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27899.1"
                     /translation="LFRGSKRDGSEWELDGPSVRAVFAHIETLKRKKRGVGNSWSSLR
                     HFRRFPEWTQPPCAAGRVFCTMDPEGVLFSCGELDRSDRSNSVVRFGVAGAFANLVRK
                     GCGECWSARLVEENYAWGCRIDRMFPPLRTPPFGRQSS"
BASE COUNT          557 a          735 c          704 g          600 t
ORIGIN      
        1 catcggcatc gacgatgcga ggttggcgga gttggggtac gattaacata cttccttttc
       61 ccccgacatc gagaatacct gtgcggagga gtaaaaccta aaagcaaggc tatttcagga
      121 tgactccgct ttcgctcgcg ccggggacga tgatacgaga ctgttcctga cgctgcatct
      181 ccttggcttc ttcgatcatt ttttccatga aactctgcat ggtctcagga aatttctcta
      241 tcgcctcatg gaggtttttc gcctcgatgg ggaactggat tggaagaggg ccgtggggcg
      301 aaaccagttg cgtctgcccg aaaaagatga ttttccggct cttgtcgact tccccgtttg
      361 tcttgaccgg ggtaagctga cggatggcgg cgacccttat gtcggtaaag acttcttcct
      421 tataaaggtt ctgctggttt atcctgactt tttgaggggt tccttcgggt gtggtcataa
      481 aaaactcctg tgtagtctac ggccgcttcc tcggttccgg cctgttccgg gtgacttagc
      541 acaattcacc ccaaatgtca ccttccgagc gctccatcac agcgggcagc atctttcagg
      601 ggaaagaaaa ggcaatgcgt cccattagtt cacaagaagg caacaaacca catggaaaac
      661 agtcggagga attcgatgag ccgtaaaaag attttccctt ttccggcaga gaggttcgac
      721 acattcgaga gtgtcctcaa aatcgacacc acctcacaga ggcatcttct ccatgtcggc
      781 gacgaggtgg agcaggtctg ggcggcctgg gcgctcggcc ttaagcttgg ggcacatatc
      841 acccctgagc tcgtcgaatg cctgaagacc tgcaggtttc ccggaacccg ccgtcacttt
      901 ttggtggtcc tcgcaggtct gggtgaaagg gagacgctgg agaaatacgc tgttaacgac
      961 cctgcgcccc ttgtccgggc cacggcctgc cgctacctgg caaggacggc agaacccggg
     1021 aacatgcgga taggacgttt tctcctcgag cgcctccggg aagatgcgtc tcccgaagtg
     1081 cgggaagcga tcctcgagga gctggaccgg ggtaaaaacg ggacggggtc cggtcaggaa
     1141 tgacggcaaa gcgggtcaga tgttctttga gatgtacttg tacatccccc tgaatacgct
     1201 ctccaggata tcgggggcga tgaagtaatc gggcatctct ttgtagaaat actcatttgt
     1261 cgccttgctc gccgcgtcga tgtcgccgtt aaacttgcga tacacgtcct ccatgtaagc
     1321 cttccatctg cggccctccc gaagagtcag gtcgatgaaa cctcccgctt cctctccggt
     1381 gatgtagccg taatggtcgg cgcagagata ggcgaccggg tagaccttca gtttttccag
     1441 actctggaga tactggtcca tgttcgtatt catcgacgga aaagacgtat ccctgtatgg
     1501 aatgcccccc gcgtcggagg cgaacatcgc cttcaggttc ggttcataag cagagatgga
     1561 acagttggta tggcccggcg tgtagatgat ctcgaggaag gcacccccga gctctattct
     1621 atcgccctct gcaagtgtct ttcccttgat gtcgtcacgc cagtcgatgt cgtaaggcgc
     1681 caactcactt tccaggccca catgcttcgc gctcagccta ctgaagctgt tgatgacctc
     1741 gatagccttg ggcatcgcga gcgtcttcca ggccggctcc gaagcgcaga cttcgatagc
     1801 gggataagcc ctcttgaaat acggaatgat gcccacgtgg tcgaaatggg aatggaggat
     1861 aagaatctta tcgatctttt cggcgtcgat tccgaactcc ttcatctgcc ggagaacatc
     1921 cggtaagatg taactgagtc cgccgttgat cagtatcgag cttgccttgc cttcgagtac
     1981 gtaaacaccg gcctcctcac ggccgaggta ccagagattg tccgcgacct ttcccgcctt
     2041 gcgaaacctc atgatcctta cccccttgct attcactgaa tatcgttgaa tatagagtgc
     2101 gtcacactat attcaagatg accgacgatg gcaagggaaa aggttgtatg ggccgaccgc
     2161 agcccctttt tcttcaagac gattggcgac cgaaaggcgg cgttcggagg ggagggaaca
     2221 tccggtcgat gcggcagccc caggcatagt tttcttccac caaccgcgcg ctccagcatt
     2281 cgccgcaccc ttttctcaca aggttcgcaa aggccccggc caccccgaat cgcactacgc
     2341 tgttcgaacg gtcgctgcgg tccagttctc cgcacgagaa cagcacgccc tctggatcca
     2401 tagtgcaaaa taccctgcct gcggcgcagg ggggttgggt ccattccggg aatcggcgaa
     2461 aatggcgcaa gcttgaccaa ctattcccca cgcctcgctt cttacgcttc agtgtctcga
     2521 tgtgcgcgaa gacagcccga acgctcgggc cgtcgagttc ccactccgaa ccgtcgcgct
     2581 tacttccccg aaaaag
//
LOCUS       DAOTMX010000258         2596 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_31010_length_2596_cov_3.110980, whole genome shotgun sequence.
ACCESSION   DAOTMX010000258 DAOTMX010000000
VERSION     DAOTMX010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31010_length_2596_cov_3.110980"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="P5573_07280"
     CDS             complement(<1..228)
                     /locus_tag="P5573_07280"
                     /inference="COORDINATES: protein motif:HMM:NF024940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRT27900.1"
                     /translation="MKDMDGRRLKHDVLTELRKRAVARVQSGESPEVVVRALGFTRAC
                     IYNWLAMYRAGGWDALDAKKRGGRPRKLQGHM"
     gene            complement(348..1202)
                     /locus_tag="P5573_07285"
     CDS             complement(348..1202)
                     /locus_tag="P5573_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HRT27901.1"
                     /translation="MMISSASPVRATEGGGNAYPNGADDFMAGALPPPGTYLLDYFLY
                     YRANSFKDKDGHDAMAKFDLNAFVNVFRIVHVTQVQFLGANWAMHVFLPLVYKDVDAT
                     FGKDNRSGIGDVIIDPFILRWHWKNFHLTTGLDIYLPTGNYNKDRLLNTSRNYYTLEP
                     IAAVTYLADNGFELTGKFMYDYNFKNDDTDYKSGQEFHFDYTIGYHINKSWAVGLGGY
                     YYYQTTDDEQYGTTYQDGNKGRVFAVGPQVAYTYKNMSFTLKWQKEFEARNRPQGDNF
                     WLKFTYAF"
     gene            complement(1459..2355)
                     /locus_tag="P5573_07290"
     CDS             complement(1459..2355)
                     /locus_tag="P5573_07290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HRT27902.1"
                     /translation="MTGERRLAITFFVTLLILAAEVIGGFLSNSLALLSDAGHVATDA
                     LAIGIGFVANRLSRRPSDRYATFGYQRVGLLAALINGVSLLVIAGFIFYESYGRLLSP
                     PEINIPLMLSIATIGLAGNSIMAFILGHGHGDLNIKSVWLHVMGDTFSSVGVILSGIA
                     IYFTGWRCLDPIISITIGGVIVWGGIRLIRDTLAIFLDLTPKGFDIKALTREITAIPD
                     VIGVHDVHLWSFAHDLVAFSAHILVSDRTLQEAEFTKERIEKILREKGIAHTTLQIEC
                     SCANCSDSLYCQQTNPREDKER"
BASE COUNT          618 a          673 c          670 g          635 t
ORIGIN      
        1 catatgcccc tgcaacttgc gagggcgacc gcctcgcttc tttgcgtcca gggcatccca
       61 accgcccgcg cggtacatgg ccagccagtt gtatatgcaa gcgcgggtga atcccaacgc
      121 ccgcactaca acctccggac tctccccgct ttgtaccctg gctactgcac gcttgcgaag
      181 ctcggtcaaa acatcatgtt taagtcttcg gccatccatg tctttcatgg acggtcttat
      241 aacacttatt tttagaactg tctatactat tatggactga ttaataattg gttaacagcg
      301 tcctgctctt ggtgacgggc cggccctggt gaatcgactt ttacttctta gaaagcatac
      361 gtgaacttga gccagaaatt atcgccctgg ggacggttcc tggcttcgaa ttctttctgc
      421 cacttcaatg tgaaagacat gtttttgtaa gtataggcaa cctggggacc cacggcaaac
      481 acgcggccct tgttgccgtc ctgataggtt gtaccgtact gctcgtcatc ggtcgtctgg
      541 tagtaatagt agcctccgag tcccacagcc caggatttgt tgatatgata gccgatcgtg
      601 tagtcgaaat ggaattcctg gcctgacttg taatccgtat cgtcattctt gaaattgtaa
      661 tcgtacatga actttccggt cagttcgaag ccgttgtccg ccagataggt cacggccgcg
      721 atcggctcga gggtatagta attccggctc gtgttcaaca gccggtcctt gttgtaattt
      781 cccgtgggca gatagatgtc caaaccggtc gtgagatgaa agtttttcca atgccagcgg
      841 aggatgaacg ggtcgataat cacgtctcca atgccggagc gattgtcttt gccgaaggtg
      901 gcgtccacgt ctttatacac caacggcaga aacacgtgca tcgcccagtt ggccccgaga
      961 aactggacct gggtcacgtg cacgatgcga aagacgttga cgaaagcgtt gagatcgaat
     1021 ttcgccatgg cgtcgtggcc gtccttgtct ttgaaggagt tcgcccggta atagagaaaa
     1081 tagtcgagca ggtacgtgcc cgggggaggc aaagcgcctg ccatgaaatc atcggcgcca
     1141 ttcggatagg cattgccgcc gccttcggtc gcccggacgg ggcttgcaga tgaaatcatc
     1201 atgatgaacg ccgccaggca ggcaataatc agtaaccgcc tgcggacaga aaaacaactc
     1261 atgcgtcaat tcctttctgt ttggaagtgg ttgctgaaat taatgaattg ttttatttca
     1321 taaaccagaa gaaaatagaa gaatatttca tctcctaccc gacgatgcgt ttgacatagt
     1381 ggaacgccct gtttaagtgg cataatgact gatcacaggt tttccccccg cgaaggctgt
     1441 tcgtttaagt gccggcggtc atctctcttt gtcctcgcgg gggttagtct gctggcaata
     1501 gaggctgtcg ctacagttag cgcagctaca ctcgatctgc agggtggtgt gtgcaatacc
     1561 cttttccctc aatatctttt cgatacgctc cttagtaaac tcagcctcct gcaaggtccg
     1621 atcgctcacc aggatgtggg cggagaatgc cactaagtcg tgggcaaagg accagaggtg
     1681 gacatcatgg acaccgatga catcgggaat ggccgtgatc tccctcgtca gcgccttgat
     1741 atcgaacccc ttcggagtta ggtctaaaaa gatagcgagc gtatccctta taagccttat
     1801 cccaccccat acgatgacgc ccccgatcgt aatgctgatg atagggtcta agcacctcca
     1861 gccggtgaag taaatggcaa tcccggataa gataaccccg acagacgaaa aggtatcgcc
     1921 catgacatgg agccacactg acttgatatt taaatccccg tgaccgtggc cgaggataaa
     1981 ggccatgatc gagtttcccg caaggccgat ggttgcaata ctgagcataa ggggtatatt
     2041 gatttccggc ggagagagga gtctgccgta cgattcatag aatataaagc cggcgatgac
     2101 gaggagactt accccattta tcagcgctgc caagagtcca acccgctggt agccgaaggt
     2161 ggcgtatctg tcggaaggcc ttctgcttaa acggtttgca acaaacccga ttcctatggc
     2221 cagcgcatct gttgccacgt gccccgcgtc gcttaagagg gcaaggctgt tactcaaaaa
     2281 gccgccgatg acctcggctg cgaggataag aagggtgaca aaaaaggtaa tagcgaggcg
     2341 tctttctcca gtcatgtcca cggcgcacca cctggtatca tacgcttttc atggaaagac
     2401 cgctcaatgg cgattctcat tatctttttc caacagctgg gtggtgatag agttattcta
     2461 ctgcattcga ggttcagtac gcaaagggag acgatccgag gtgctctcga tcctgatatc
     2521 gattatggac catgactgat cgaaacccat ttcccgacgc catcacgcag gccgacgtat
     2581 tttctggcct tggaga
//
LOCUS       DAOTMX010000259         2579 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_31275_length_2579_cov_4.383914, whole genome shotgun sequence.
ACCESSION   DAOTMX010000259 DAOTMX010000000
VERSION     DAOTMX010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31275_length_2579_cov_4.383914"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="P5573_07295"
     CDS             complement(<1..272)
                     /locus_tag="P5573_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27903.1"
                     /translation="MKSGKITPSRTFWASVPSWLIIGCALILVPIFVVMTLQSIAKQR
                     ELTTRLLIEKGEAIIRSFEAGSRTGAGMQWGSFQLQKLLIETAEQPG"
     gene            655..1392
                     /locus_tag="P5573_07300"
     CDS             655..1392
                     /locus_tag="P5573_07300"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRT27904.1"
                     /translation="MRIKVLGTRGEVEPSSPYHAKHSGVLVDGEVLFDLGEREFLDLN
                     PRVVFITHLHPDHAFFVNEPAEVSVPLYGPEAFGKNPAVDIHVLEAAAEFDAYCVTPI
                     PTHHSLRVKSQAYLLQKGEERILYTGDMVWIDKRYHPLLNGLDLVVTEGSFDRRGGRV
                     NRDAKTGKVYGHTGVPNLVRLFREFTGNILITHFGTWFYEDPRGAVRKLKELGRENGV
                     NVIAGYDGLEFDITDLTNKIKNDLTLD"
     gene            1584..>2579
                     /locus_tag="P5573_07305"
     CDS             1584..>2579
                     /locus_tag="P5573_07305"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HRT27905.1"
                     /translation="MSTDLTFITNERNKNLLERFKVLIKDTRFFDVLVGYFYSSGFHS
                     LYKALEQTEKIRILVGISTDRQTVDLIYKSRHTLQAPLQFSHAEAKETFSETIAAEME
                     ASEDTADIEEGVSKFLEWIKSGKLEIRAYPSENIHAKVYIMTFADGDRDAGRVITGSS
                     NFTAAGLVENLEFNVELKNRADYDFAHSKFNELWENAVDVRDKYLETIRTKTWLNGTI
                     TPYELYLKFLYEYFKNKINIDQDEIYRRYLPDNFLDLEYQSEAVKDAKNKLDEYGGVF
                     ISDVVGLGKTYISAMLAQQLDGRSLVIAPPMLLEKEYPGSWPNVFSGLHPPSWTKY"
BASE COUNT          650 a          715 c          661 g          553 t
ORIGIN      
        1 cccggctgct cggccgtttc aatcaggagc ttctgaagct gaaaactccc ccattgcatc
       61 cccgcccccg tgcggctccc cgcctcaaag gaccggatga tcgcctcgcc cttttcgatg
      121 agaagcctgg tcgtgagttc cctctgtttc gcgatgctct ggagggtcat gacgacgaag
      181 atagggacga ggatgagcgc acacccgatt atcagccagg aaggaacgga cgcccagaag
      241 gtccgggacg gtgtaatctt tccgcttttc atgttgacaa acccgccgcc ctccgaatca
      301 acacatttca gcgtaacatt ttagaatgac tcgacatctg ttagattttc ccttgacatc
      361 ccttgcatag agtgccatga accatcctca ttaaacaggt ttagaatgat tcggcggcat
      421 cgagacgtcc ctcatatgcc gacggaatca ttgtacggtt atatccttcg aggatttacg
      481 attcaaggtt ttgttgaata atccatcaag aggcagctta tcccgagggt gagcgtccat
      541 gcccgggacg ttcgtagaac gccgaagaat cgacgggggt cgaggggtgg atgccagacc
      601 cacgacaagc tataaggtcc tgaaggagca aaaatctgcc ctggagcatg cgaaatgagg
      661 atcaaggtcc tgggaacgcg gggggaggtg gagccatcca gtccttacca tgctaaacat
      721 tcgggcgtcc tcgtcgacgg tgaggtcctc ttcgacctcg gcgagaggga gtttctcgac
      781 ctcaaccctc gcgttgtctt catcacacac ctgcatcccg atcacgcctt cttcgtgaac
      841 gaacctgccg aggtctccgt ccctctctac gggccggaag cgttcgggaa aaaccccgcc
      901 gtggacatcc acgtcctcga ggcagcggct gaattcgacg cctactgtgt cacgccgatc
      961 cccacgcacc acagcctgcg ggtgaagtcg caagcctatc tcctgcaaaa aggagaagaa
     1021 aggatacttt acacgggcga catggtctgg atcgacaaac gataccaccc cttactgaac
     1081 ggcctcgacc tcgtcgtgac cgagggtagc ttcgaccgcc gcggagggcg ggtcaaccgc
     1141 gacgcgaaga cggggaaggt ttacggccac acgggcgtcc cgaacctcgt ccgcctcttc
     1201 agagaattca ccgggaacat cctcataacc catttcggga cctggttcta cgaggacccg
     1261 cggggagccg tgaggaaact gaaggagctc ggccgggaaa acggcgtcaa cgttattgcc
     1321 ggctacgacg gcctggagtt cgacataacc gacctgacga acaagattaa aaacgattta
     1381 actcttgact aaaccacctc cttttggtta ccctcgttta tctgaagatc accggttgtt
     1441 taataaattc caacccttca atggttcgcg aaagaaagcc atgaacggat cgtcctggaa
     1501 accccgcgac tcgaaagaga agcggggttt agttttgggt tcgctgtgct gcaaatagtc
     1561 gatggtggga tggcttgcaa atcatgtcta cagacctaac cttcataacc aacgagagaa
     1621 acaagaatct tttagagcga ttcaaggttc tcatcaagga cacccgcttt tttgacgtac
     1681 ttgtcgggta tttctactca agcgggtttc acagccttta taaggcgctg gagcagaccg
     1741 aaaagatcag gattcttgtc gggataagca ccgaccgaca gacggtcgat ttgatttaca
     1801 aatcccggca cactttgcaa gcccctctcc agttctccca tgctgaagct aaagaaactt
     1861 tctcggagac catcgccgcc gagatggaag cttcagaaga taccgcagac atcgaagaag
     1921 gcgtgtctaa attccttgaa tggatcaaga gcggaaaact tgaaatcagg gcctatcctt
     1981 ctgaaaacat ccacgcgaag gtctacatca tgacctttgc cgacggcgac cgcgacgcag
     2041 gccgggtgat caccggctcc agcaatttca cggcggcggg cctggtcgaa aatctcgaat
     2101 tcaacgtgga gcttaaaaac cgggccgact atgattttgc ccattcaaag ttcaacgaac
     2161 tctgggaaaa cgccgtcgac gtaagagaca aatatctgga gacgataagg acgaagacct
     2221 ggttgaacgg caccatcacg ccttatgagc tttatctgaa attcctctac gaatacttca
     2281 agaacaagat caacatcgac caggatgaaa tttatcggcg gtacctgccg gataatttcc
     2341 tcgaccttga gtatcagtcc gaggccgtta aagacgccaa aaacaagctg gatgaatacg
     2401 gcggagtctt catctccgac gttgtcggcc tcgggaagac ctatatatcg gccatgctgg
     2461 cccagcagct tgacggccgg tccctcgtga tcgcaccgcc gatgcttctg gaaaaagaat
     2521 atcccggttc ctggccgaac gtgttttctg gactgcaccc cccaagttgg acaaaatat
//
LOCUS       DAOTMX010000260         2562 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_31557_length_2562_cov_2.711209, whole genome shotgun sequence.
ACCESSION   DAOTMX010000260 DAOTMX010000000
VERSION     DAOTMX010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31557_length_2562_cov_2.711209"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="P5573_07310"
     CDS             <1..491
                     /locus_tag="P5573_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     II"
                     /protein_id="HRT27906.1"
                     /translation="IYAEVAGFGLTSDAYHVAAPPPGHEGAARSMELAMEEAGLAPQD
                     IDYVNAHGTSTPLNDLYEVQAIKAVFGESAHSIAVSSTKSMTGHLLGAAGGLEAVITA
                     KTIEEGVLPPTVNLDNPDDECDLDFVAHRARKREVRAALSNTFGFGGVNAVLAFKKYA
                     EG"
     gene            538..1056
                     /gene="rpiB"
                     /locus_tag="P5573_07315"
     CDS             538..1056
                     /gene="rpiB"
                     /locus_tag="P5573_07315"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HRT27907.1"
                     /translation="MGCVIIGADHAGFVLKEGLKKVLAEKGLQVIDAGTDNGEAVDYP
                     DFAEAVAEEVSAGRVPWGVLVCGSGVGMSIVANRFPGVRAALCLDEDTARMSRRHNDA
                     NVLVLAGRKTDLNTAEKIVTVWLETGFEGGRHQARLDKVVQVEKRIITRAGKGIPFGT
                     RDSMDRCGIRKA"
     gene            1066..1533
                     /locus_tag="P5573_07320"
     CDS             1066..1533
                     /locus_tag="P5573_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HRT27908.1"
                     /translation="MAKTISTQQYERPTWDEYFMDIVRLVSRRSTCLRRSVGAVLVKD
                     KRILATGYNGAPSGLRHCVEVGCLREEMNISSGERHELCRGLHAEQNAIIQSALHGVS
                     LKDATLYSTNHPCVICAKMIINAGIARVYYAEGYRDRMAEEMLLEAGVEVAKI"
     gene            1530..2009
                     /gene="nrdR"
                     /locus_tag="P5573_07325"
     CDS             1530..2009
                     /gene="nrdR"
                     /locus_tag="P5573_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HRT27909.1"
                     /translation="MKCPFCSGVENRVIDSRISKDGNAIRRRRECARCAKRFTTYEFV
                     EEVLPMVVKKDGRREPFDRNKILVGIKKACEKRPISVDVIEEVADRVEQACQDYPDKE
                     IPSAVIGERVMAELHDLDEVAYVRFASVYRQFRDADEFLEELEDLRKKKTDTLKTSS"
     gene            2023..>2562
                     /locus_tag="P5573_07330"
     CDS             2023..>2562
                     /locus_tag="P5573_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27910.1"
                     /translation="MFTGIIEGMGVVKRMTLRGVDVLLELETDMDVKSLRAGDSVCVN
                     GACLTVTSFSERGFTADVSAETLSRTNLGSLKAGDRVSCGEPLPLWKRTIGFSCPESL
                     RPVRDPEAPIRAVLGPQDDAFTPEALEVFKSKVNVRILQIDLPPGGDRPWDQGRNAID
                     MKRIGSGILLQTADNHVLQR"
BASE COUNT          566 a          676 c          849 g          471 t
ORIGIN      
        1 agatatacgc ggaggttgcg ggattcggcc tgaccagcga cgcctaccat gtggcggcac
       61 cgccgccggg ccacgagggc gccgcgagga gcatggaact cgctatggaa gaggcgggtc
      121 tcgccccgca ggacatcgac tatgtaaacg cccacgggac atcgacgccc ctcaatgatc
      181 tctacgaggt tcaggctata aaggccgtct tcggtgagag cgcccattcg atagccgtca
      241 gttccacgaa atccatgacg ggtcatcttc tcggggctgc cggtggactg gaggcagtca
      301 taactgccaa gacaatcgag gagggggtcc tgccgccgac ggtcaatctg gacaaccctg
      361 acgatgagtg tgacctcgac ttcgttgccc accgagccag gaaaagggag gtgagggccg
      421 ccctgtcgaa caccttcggg tttggtgggg tgaacgccgt gctggcgttc aaaaaatacg
      481 cggagggttg agggcgcggg gcaaaagaaa gagttgaggg ctgactgctt acaaaccatg
      541 ggttgtgtca tcatcggggc ggaccatgcg ggattcgtcc tgaaagaggg attgaaaaag
      601 gttctggcgg agaagggtct gcaggtaatc gacgcgggaa cagacaatgg ggaggccgtc
      661 gattatcccg attttgccga agcggtggcc gaagaagtct ccgcgggaag ggtgccgtgg
      721 ggcgtcctgg tctgcgggtc gggcgtaggt atgtcgatcg tagcgaaccg cttcccgggg
      781 gttcgggcgg cactttgcct cgacgaggac acggcgcgga tgagccgtcg ccacaacgat
      841 gccaacgtcc tcgtccttgc ggggcggaag acggacctta acacagcgga aaagattgtc
      901 acagtctggc tggaaacggg attcgaagga ggtaggcacc aggcgagact cgacaaggtt
      961 gtgcaggtcg agaaaagaat aataacccgg gccgggaaag ggataccatt cggcacgaga
     1021 gactcaatgg ataggtgcgg tatccgcaaa gcctgaagag gttttatggc taaaaccatc
     1081 agcacccagc agtacgaacg accgacctgg gacgagtatt tcatggatat cgtccgactc
     1141 gtcagtcggc ggtccacttg tttgaggcgg agcgtcggcg cagttctggt aaaggacaag
     1201 cgtatcctgg cgacgggtta caacggcgcg cccagcgggt tgcgccactg tgtggaagtc
     1261 ggttgcctcc gggaggagat gaacatatct tcgggagagc gccacgagct ctgccgtggg
     1321 ctgcacgccg agcagaacgc aataatccag tcggcccttc acggtgtcag ccttaaagac
     1381 gcgacccttt acagcacaaa ccatccctgc gtcatctgcg ccaagatgat catcaatgcc
     1441 gggatcgccc gcgtttatta cgcggagggt tatcgcgaca ggatggcgga agaaatgctt
     1501 ctcgaggccg gcgtcgaggt ggccaagata tgaagtgccc tttctgttcc ggcgtggaaa
     1561 accgggtgat cgattcccgg atcagcaaag atggtaacgc tatccgcagg cggcgtgaat
     1621 gtgcccgatg tgctaaacgg ttcacgactt acgagttcgt cgaggaggtc ctgcccatgg
     1681 tcgtcaagaa ggacggccgg cgggagcctt tcgaccgcaa taagatcctt gtcgggataa
     1741 agaaggcctg cgagaaacgc cccatcagcg tcgacgtgat agaagaggta gcggatcggg
     1801 tcgagcaggc ttgccaggat taccccgaca aggaaatacc ttccgccgtt atcggagaaa
     1861 gggtcatggc ggaactgcat gaccttgatg aagtggctta tgtgcggttc gcttcggtgt
     1921 accgtcagtt ccgtgacgcc gatgagttct tggaagagct ggaagacctg aggaagaaga
     1981 agacggacac ccttaagacg agcagttagc gggggcggag agatgtttac gggtatcata
     2041 gaggggatgg gcgtcgtcaa gagaatgaca ctgcggggcg tggatgttct tctggagctg
     2101 gaaaccgaca tggatgtaaa gtcccttagg gccggcgaca gcgtctgtgt gaatggggct
     2161 tgtcttaccg taacatcctt tagtgaaagg ggatttaccg ctgacgtctc ggcggagacg
     2221 ctctcgcgga ccaacctcgg cagcctgaag gccggcgaca gggtttcctg cggggagccc
     2281 ctgcccctct ggaaaaggac gatcggtttt tcctgccccg aatccctccg gccggttcga
     2341 gacccggagg ctcccatccg ggcggttttg gggccccagg acgatgcctt cacgcccgag
     2401 gccctggagg tcttcaagag caaggtcaac gtgcgcatcc tgcagatcga ccttccgccc
     2461 gggggcgatc gtccctggga ccaggggcgc aacgccatcg acatgaaacg catcggctcg
     2521 ggcatcctgc tgcaaaccgc cgacaaccac gtgctgcagc gc
//
LOCUS       DAOTMX010000261         2510 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_32425_length_2510_cov_3.259878, whole genome shotgun sequence.
ACCESSION   DAOTMX010000261 DAOTMX010000000
VERSION     DAOTMX010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32425_length_2510_cov_3.259878"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..918)
                     /locus_tag="P5573_07335"
     CDS             complement(<1..918)
                     /locus_tag="P5573_07335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRT27911.1"
                     /translation="MKRGIFDSAKIDEINEKTAEWEKNRYGGRRQLKNKYKTLSGFDV
                     KPLYTPGDVVDFDYLQDLGYPGEGPFTRGVHPTMYRGRTWTHRQLAGFGPPEETNKRY
                     KFLLQQGATGINGVFDYPTLRGYDSDDKDVYADVGRGGVAIDTVEDMRILFDGIPIDE
                     VSVSLVTCQPICNISIQSMYFANAGDRGIPLTSLAGTSQNDFLLETAITVAPGVLPPR
                     ASFKVSCDAIEFCTRHAPRWNPISFAGYNYREAGCTAPQEVAFVIAHAVACSEEMLRR
                     GLPVDSFAGRLSFFLSAHNDFFEEIAKYRA"
     gene            complement(1139..>2510)
                     /locus_tag="P5573_07340"
     CDS             complement(1139..>2510)
                     /locus_tag="P5573_07340"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRT27912.1"
                     /translation="IIDDQKLIRDSLRLALKDEGYEVFAAKTGKEGLDVLEKENTDLI
                     LLDLKLPDTNGIDILKRIKTTDPDAIVIMMTGYGTVENAVTAMKLGAFDYVNKPFEYK
                     NISTIIKLALETRKLQKEVNDLREKAKSLYEADKIVGKSEETQTIRGIIAKVSQSGSS
                     TVLIEGESGTGKELVAKAVHYNSPRSNGPFVEINCSAIPYSLLESELFGYEQGAFTDA
                     KRSRKGLFEQADGGTLFLDEIGDMDLAMQSKLLNVLQEKKFRRLGGHRTIQVDTRIIA
                     ATNHNLRDYVRRNKFREDLYYRLNVLGIYIPPLRERKEDILPIAEYFIEILNRRYRKR
                     VQGIDEEAANAMVNYTWPGNVRELGNTIERIMIIESPDVITLKNLPREILEGSSPEPR
                     LGIDELGLPDSGVDIKELTHALQTKLIKQALIKAKGNRTEAARLLNLDRFSLRYLMNK
                     YKLIDL"
BASE COUNT          491 a          700 c          669 g          650 t
ORIGIN      
        1 ggcacggtac tttgcgatct cctcgaaaaa atcattgtgg gccgacagga agaagctgag
       61 cctgccggca aagctatcga ccggcaagcc cctcctcagc atttcctcgg agcaggcgac
      121 ggcgtgggcg atgacgaagg cgacctcctg cggggccgtg catccggcct cacggtagtt
      181 gtacccggcg aagctgatgg gattccatcg gggtgcatgc ctggtgcaaa attctatggc
      241 atcgcaactg accttgaaag aggccctggg cggtagaacg cccggagcta cggtgatcgc
      301 cgtttccagc agaaaatcgt tctggctcgt ccctgccaaa cttgttagcg ggatgccgcg
      361 gtccccggca ttggcaaagt acatcgattg gatggagatg ttgcagatag gctggcatgt
      421 caccagggag acgctgacct cgtcgatggg aatgccgtca aagaggatcc gcatatcttc
      481 gacggtgtct atcgccaccc cgccccttcc cacgtccgca taaacgtcct tgtcgtcgct
      541 gtcgtaaccg cgcagagtgg ggtagtcgaa aaccccgttg atccccgtgg cgccctgctg
      601 caggagaaat ttgtacctct tgttcgtctc ctccggggga ccgaaaccgg ccagttgccg
      661 atgagtccat gtcctccccc tgtacatcgt cggatgaacg ccgcgggtaa aggggccctc
      721 ccccggataa cccaggtcct gcaaataatc gaagtccacc acatcgcccg gcgtatacag
      781 gggtttgacg tcgaacccgg aaagcgtctt gtacttattt tttagctgcc ttcggcctcc
      841 atacctattc ttttcccatt ccgctgtctt ttcattgatc tcatcgattt tagccgagtc
      901 aaaaattccc cttttcatcc ctacctccct gaggatcgtt tctgacctga ccggtgcacc
      961 aattatgcca cgcaaaggcg agtctctccc cggattctct tttttgcatc gttcacttgt
     1021 attgggcgtg gtgcattgcg gcttcgggag aggtccggga atgcggtggg gaacggccct
     1081 ggagggcaga atacctttta gatgtggaaa atatgcacgg ggcgtgaaaa taatttactc
     1141 ataggtcaat cagtttgtac ttgttcatca aatagcgcag ggaaaagcgg tcgaggttca
     1201 gaagccttgc cgcctctgtc ctgttcccct ttgccttgat aagggcctgt ttgatcagtt
     1261 tcgtctgcaa ggcatgggtg agttccttga tgtcgacccc cgaatctggc aatcccagct
     1321 catcgatgcc gagccgtggt tccggactgc tcccttccag gatttcccgc ggcagattct
     1381 tgagcgttat gacatcaggg ctttcgatga tcatgatccg ctcgatggta ttgcccagct
     1441 cccggacatt gcccggccac gtatagttga ccatggcgtt cgcggcctct tcgtcgatac
     1501 cctgcaccct ctttcgataa cgcctattca ggatctcgat gaagtattcc gctatcggaa
     1561 ggatgtcctc ttttcgttct cttaagggag gtatgtagat gccaaggacg ttcaaccgat
     1621 agtagaggtc ctcccggaat ttgttccggc ggacgtaatc acgcaaattg tggttggtcg
     1681 cggcgatgat gcgcgtatcg acctgtatcg ttcgatgccc tcccagacgc cggaatttct
     1741 tctcttgcag cacgttcaac agtttggatt gcatcgcaag gtccatgtca ccgatttcat
     1801 cgagaaacaa agtcccccca tccgcttgtt cgaaaagacc ttttcggctt cgtttggcat
     1861 cggtgaatgc cccttgttcg tagccgaaca gttcactctc caggagggaa taggggatgg
     1921 ccgaacagtt gatttcgaca aaaggcccgt ttgaccgcgg gctgttgtaa tgaacggcct
     1981 tggcgacaag ctccttgccg gtcccgcttt cgccctcgat caggacggtg gatgaaccac
     2041 tctgggatac cttcgcgatg ataccccgga tggtctgagt ctcttcgctc tttccgacga
     2101 ttttatcggc ctcgtaaagc gacttggcct tctctctgag gtcgttcacc tctttttgta
     2161 acttccgcgt ctccagggca agcttgatga tggtggaaat gtttttgtac tcgaagggtt
     2221 tgttgacgta atcgaaggca ccaagcttca tcgcggtgac ggcattctcg acggtcccgt
     2281 agccggtcat catgatgacg atggcatccg ggtccgtcgt cttaatccgc ttgagaatgt
     2341 cgattccgtt cgtatccggc aatttgaggt ctaagaggat caggtccgtg ttttcttttt
     2401 ccaggacgtc cagaccttct ttgcccgtct tcgccgcgaa cacttcatag ccttcgtcct
     2461 tgagggccaa tctgagagaa tcccggatca gtttctggtc gtcgattatg
//
LOCUS       DAOTMX010000262         2486 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_32813_length_2486_cov_3.451666, whole genome shotgun sequence.
ACCESSION   DAOTMX010000262 DAOTMX010000000
VERSION     DAOTMX010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32813_length_2486_cov_3.451666"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /gene="trmD"
                     /locus_tag="P5573_07345"
     CDS             complement(<1..564)
                     /gene="trmD"
                     /locus_tag="P5573_07345"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HRT27913.1"
                     /translation="MVRFDILTLFPGMFASTFESSLLKKAQEKGLIEIRVHDIREYTE
                     DRHRMADDYPYGGGSGMVMKVEPIARALEHIFPDRESAVVIMLSPQGEVFNQRTALEL
                     SKASRLVMVCGHYEGVDERVGTHLVDREISIGDYVLTGGEVPAMVVVDAVARLVPGVL
                     GNRESAQTDSFSSALLEYPQFTRPAEYR"
     gene            complement(570..1070)
                     /gene="rimM"
                     /locus_tag="P5573_07350"
     CDS             complement(570..1070)
                     /gene="rimM"
                     /locus_tag="P5573_07350"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HRT27914.1"
                     /translation="MELFILGRVTTSHGLKGGMRVFSYLESDGTLARLTEVVLRKKGE
                     EGRVFKVRGVKPKGKTFILTVEGIEDAEAAGRWFGADVFASSSILEELPPGEYYWKDL
                     IGLRVVTEEGRYLGRVEDIMPTGSNDVYVCRGGEREILLPAVEDVVRKVDIDEGTMVV
                     KLYKGL"
     gene            complement(1074..1328)
                     /locus_tag="P5573_07355"
     CDS             complement(1074..1328)
                     /locus_tag="P5573_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HRT27915.1"
                     /translation="MVSVEVLTMKDLIKYIAQALVDNPDAVEVSEVLGEQTSVIELRV
                     AKEDLGKVIGKGGKTAKAMRTILSAASAKMKKRAVLEIVE"
     gene            complement(1386..1646)
                     /gene="rpsP"
                     /locus_tag="P5573_07360"
     CDS             complement(1386..1646)
                     /gene="rpsP"
                     /locus_tag="P5573_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HRT27916.1"
                     /translation="MAIKVRLVRVGAKKKPFYRIVVANSESPRNGRFIEIVGNYDPKK
                     EPAEVNIKEDRVREWLSKGAIPTVTVSSLLEKKGIQAGMKAQ"
     gene            complement(1716..>2486)
                     /locus_tag="P5573_07365"
     CDS             complement(1716..>2486)
                     /locus_tag="P5573_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HRT27917.1"
                     /translation="DTAGRLHIDTEMMDELKRIQDVVRPSEILLVADAMAGQDAVSVA
                     EKFDQLLGIDGVILTKMDGDARGGAALSLKAVTGKPIKFVGVGERVDALEVFHPERMA
                     SRILGMGDVLTLVEKAKAAIDEREAKELEKKIRKDTFTLEDFRNQLLQVRKMGSLRDL
                     IGMIPGLNKVKAVKDLAPDEKDLVKTVAIIDSMTRKERLNYQIIDGRRRKRIARGSGT
                     TVQDVNRLLKNYAEARKIMKRMTKGGVKALRRGNFPFP"
BASE COUNT          490 a          813 c          561 g          622 t
ORIGIN      
        1 ccggtattca gcgggccgcg tgaactgtgg gtattccagg agcgccgatg agaaagaatc
       61 cgtctgggcg gattccctgt ttcccagcac cccgggtacc agacgggcaa cggcgtcgac
      121 aaccaccatg gccggcacct ctcctcccgt gaggacgtaa tcgccgatcg aaatttccct
      181 gtcgaccaga tgggttccca cccgctcgtc cacgccctcg taatgcccgc agaccatgac
      241 cagacgggaa gccttcgaca actctaaggc cgttcgctga ttgaagacct ctccctgcgg
      301 cgagagcatg atcacgacgg ctgattccct gtcggggaag atatgttcga gggcgcgggc
      361 tatgggctcc actttcatca ccatcccact tccgccgccg tagggatagt catcggccat
      421 ccgatgcctg tcttcagtat actccctgat gtcgtggact cttatctcga taagcccctt
      481 ctcctgggcc tttttcagga gactcgactc gaaggtcgac gcaaacatcc cagggaagag
      541 cgtgagtatg tcaaacctga ccatccgttt tacaaaccct tatacagctt gaccaccatg
      601 gtgccttcat cgatgtcaac cttgcggacc acatcttcaa cggcgggcag aagaatctcc
      661 ctctcccctc ctctgcagac atacacgtcg ttgctgcccg tgggcattat atcctcgaca
      721 cgccccagat atcgcccctc ctccgtcacg accctcaatc cgatcaggtc tttccagtag
      781 tattcgccgg gcgggagctc ttccagtata gaggaagacg caaaaacatc ggcgccgaac
      841 caacgtccgg ccgcctccgc gtcttcgatt ccttccaccg tcaggatgaa tgttttgccc
      901 tttggcttga caccgcggac cttaaacacc cttccctctt cccccttttt tcggagaacc
      961 acctcggtga gccgcgccag cgttccatcc gattccaggt atgaaaatac cctcatacca
     1021 cccttcagac catgggatgt cgttacccga ccgaggataa aaagttccac gacctactcg
     1081 actatctcca ggactgccct cttcttcatc ttggcggaag cggcgctgag gattgtcctc
     1141 atcgccttcg ccgtcttccc acctttgccg atgaccttac caaggtcctc cttggcgacc
     1201 ctcagctcga tcaccgatgt ctgctcccct aaaacttcgg aaacctccac ggcgtccggg
     1261 ttatcgacca atgcctgggc aatgtacttg atcaagtcct tcatcgtcag cacctccacc
     1321 gataccatac cggttcgctt cgtagtatta tgagcgtttc agcaagagag cccgactgct
     1381 aaaacctact gggccttcat tccagcctgg atacctttct tttcgagtag acttgaaact
     1441 gttaccgtgg gtatggcgcc cttggacaac cactctctga cacggtcttc cttgatgttg
     1501 acttccgccg gttctttctt ggggtcataa ttgcctacga tctcgatgaa tctgccgttt
     1561 ctcggagatt cggagttcgc tacgacgatc ctgtaaaagg gcttcttttt cgcgcccacc
     1621 cgcaccaacc ttactttaat cgccattcct tcctgtcacc acctcctgaa gaattctcca
     1681 atcgaacaac gttgtaattt agcataactt ttctatcatg ggaaggggaa attccctcgc
     1741 cggagcgcct tcaccccgcc cttggtcatt ctcttcatga tcttccttgc ttccgcataa
     1801 ttcttgagaa gtctgttgac atcctgaacg gtcgtcccac tgccgcgggc gatccttttc
     1861 cgcctccgac cgtctatgat ctggtagttc aatcgttctt ttctcgtcat agagtcgatg
     1921 atggcgaccg tcttcaccag gtctttctca tcgggtgcaa ggtccttcac cgccttgacc
     1981 ttgttgaggc ccggtatcat gcctatcaga tcccgcagcg aacccatctt cctgacctgc
     2041 aagagctggt ttctgaaatc ttccagcgtg aaggtatcct tccggatctt cttctccagc
     2101 tcctttgctt ctctttcgtc gatggccgcc ttggcctttt cgaccaaagt aaggacatcg
     2161 cccatgccga gtatccggga ggccatcctc tcgggatgga atacctcgag ggcatctacc
     2221 ctctcgccga ctccgacgaa tttgatcggc tttcccgtca cggccttgag cgaaagtgcg
     2281 gccccgcccc gggcatcgcc gtccatcttc gtcaggatca ctccgtctat ccccaaaagc
     2341 tggtcgaatt tttccgcgac cgaaacggca tcctgaccgg ccatggcgtc ggcaaccagc
     2401 agtatttcgg acggacggac gacatcctgg atcctcttca attcatccat catctcggtg
     2461 tctatatgca aacgaccggc cgtgtc
//
LOCUS       DAOTMX010000263         2484 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_32858_length_2484_cov_3.396871, whole genome shotgun sequence.
ACCESSION   DAOTMX010000263 DAOTMX010000000
VERSION     DAOTMX010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32858_length_2484_cov_3.396871"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="P5573_07370"
     CDS             complement(<1..205)
                     /locus_tag="P5573_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27918.1"
                     /translation="MPTGVPGSNNLPAVVKGKNQSALSQTSGSQKKKEFKSDATKALS
                     GTDKAAFPVTFLNVLGEVGTLAGR"
     gene            complement(403..1014)
                     /locus_tag="P5573_07375"
     CDS             complement(403..1014)
                     /locus_tag="P5573_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27919.1"
                     /translation="MSSESNSTKGTKVKKVIIACEVLVFFLFIAKMLAVGGIIRKAET
                     HFTLFPLNAAQADATVAAAGGLPVRDVLDNGLANEKKLMDHLLERQKQIESREGMLKA
                     EEKKLEALKQEVVAKIDNLRALEEKLSVSLDAEDKKLKDLAKVYEATPPPQVGSIMEK
                     MDKKMAAAIIANMDTKKAGKVWAHISPGKAVEIAKEMAKFKHE"
     gene            complement(953..1414)
                     /gene="fliJ"
                     /locus_tag="P5573_07380"
     CDS             complement(953..1414)
                     /gene="fliJ"
                     /locus_tag="P5573_07380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HRT27920.1"
                     /translation="MFKFALQPLLDFRRRIEEKALTEYAGQVRRFETEKERLERLRHE
                     KSLLARRFSEMQQGVMKAGDVATLFAYLGRVSSEERGQEDVVRKAAEELEEKRQNLLE
                     AVKKRKAIEVLRDRQHEAYRQALVRKELRRLDEFGIEQHKRNEGKEGHHRL"
     gene            complement(1420..>2484)
                     /locus_tag="P5573_07385"
     CDS             complement(1420..>2484)
                     /locus_tag="P5573_07385"
                     /inference="COORDINATES: protein motif:HMM:TIGR01026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HRT27921.1"
                     /translation="RIVARGRRADAKVGRKLLGRVINGLGNPMDGNGPLEYEGSYSLY
                     ADPINPLWRGRITSPLDLGVRVINGLLTCGKGQRMGIFSGSGVGKSVFLGMIARNTKA
                     DVNVIGLIGERGREVREFLEKNLGKEGLARSVVIVATSDTHPLIRMRAAYLATTIAEY
                     FRDLGKDVLLMVDSLTRFAMAQREVGLSVGEPPTTKGYTPSVFSMMPKLLERAGTLEG
                     RGSITGIYTVLVEGGDFDEPIADAARSILDGHISLSRRLAARNHYPAVDVLNSISRVM
                     VDVVDAEHKAKANDVLNIVATYSNAEDLINIGAYVDGSNPEIDHALRMIGKVNAFLKQ
                     GIEERVAFEDSRRELFALFE"
BASE COUNT          431 a          790 c          652 g          611 t
ORIGIN      
        1 ccctaccggc aagggttcca acctctccaa ggacattcaa gaaggtcaca gggaaggcgg
       61 ccttgtccgt cccggacagg gccttcgtcg cgtcgctctt gaactccttc ttcttttgag
      121 acccggacgt ctgcgacaaa gccgactgat tctttccttt cacaaccgct ggcagattat
      181 tagaccccgg gacaccggta ggcatagagt gcttcctggt attttttaac tatcgagaag
      241 gcaagagctg tgccacaggc cgctcgttgc cgaaacctat catttatcag tgacttaacg
      301 gttaggggag ctgctcgtct ttttggggag gggaaaattt ttccggatag gccggggaac
      361 ggacgtccca aaaaaaccta cccggaaatc aggggggtgc attcactcgt gtttgaactt
      421 ggccatctct ttggcgatct cgacggcctt gccggggctt atatgggccc ataccttacc
      481 ggcttttttg gtgtccatgt tagcgattat cgccgccgcc atctttttgt ccattttttc
      541 catgatggag ccgacctggg gtggaggcgt tgcctcatac accttggcga ggtctttgag
      601 tttcttgtct tcggcgtcga gggaaacact gagtttttcc tccagggccc gcaggttgtc
      661 tatttttgcg acgacctcct gcttcaaggc ctcgagcttc ttctcttccg ctttcagcat
      721 accttcccgc gattctatct gcttctgccg ttcgagcagg tggtccatca gcttcttctc
      781 gttcgccagg ccgttgtcca gcacatccct cacgggaagc cctcccgcgg ccgccacggt
      841 ggcatccgcc tgcgccgcat tgagcgggaa gagtgtgaag tgggtctccg ccttcctgat
      901 gatcccgccc acggcaagca tcttggctat aaagagaaaa aataccagga cctcacaggc
      961 gatgatgacc ttctttacct tcgttccttt tgtgctgttc gattccgaac tcatccagcc
     1021 tcctaagctc ctttcgtacc agtgcctgtc tgtatgcctc gtgttgcctg tctctcagga
     1081 cctcaatcgc cttccgtttc ttcaccgcct ccagaagatt ctgcctcttc tcctccagtt
     1141 cctccgccgc cttccttacg acgtcttcct ggcccctctc ttcactgctg acccgaccga
     1201 ggtaggcaaa aagcgtcgcg acatcgcccg ccttcatcac gccttgctgc atctcggaga
     1261 aacgccgtgc caacagggac ttttcgtgcc gtagcctctc gagtctttct ttctccgtct
     1321 cgaaccgtct gacctgtccg gcgtactcgg tcagggcctt ttcctcgatg cgccgacgga
     1381 aatcgagcag gggctgcagg gcgaacttga acatggactt cactcgaaca aggcgaagag
     1441 ctcacggcga ctgtcttcga aggcaacccg ttcttctatg ccctgtttca ggaaggcgtt
     1501 gaccttgccg atcatccgga gggcgtggtc tatttccggg ttgctcccgt ccacgtaagc
     1561 gcctatgttg atcaggtcct cggcattact gtacgtggcg acgatgttca ggacatcgtt
     1621 tgcctttgcc ttatgctctg cgtccaccac gtcgaccatc accctgctta tgctgttgag
     1681 tacatcgacg gcggggtagt ggttcctggc ggccagccgg cgggaaaggg atatgtggcc
     1741 gtccaggatc gacctcgccg catcggcaat cggctcgtcg aagtcaccgc cctctacgag
     1801 gactgtgtag atgcccgtta tgctgcctct cccctccagc gtcccggccc tctccaggag
     1861 tttcggcatc atgctgaaga cggaaggcgt ataccccttc gtcgtcggcg gctcgccgac
     1921 cgaaaggccg acctcgcgct gggccatcgc aaacctggtc agggaatcga ccatcaggag
     1981 gacatccttt cccaggtccc ggaaatattc cgctatggtc gtcgccaggt atgccgccct
     2041 catcctgatc aggggatggg tgtccgacgt ggcgacgata acgacggacc tcgccagacc
     2101 ttcctttccc aggttttttt cgaggaactc tctcacctcc cggccccttt cgccgatgag
     2161 gccgatgacg ttcacgtccg ccttcgtatt gcgggcgatc atccccagga agacactttt
     2221 ccctacgccc gacccggaaa agatacccat ccgttgcccc ttgccgcagg tcagcaggcc
     2281 gttgatgacc cgaacgccga ggtcgagggg gctcgtgatg cgacccctcc agagggggtt
     2341 tatcgggtcg gcgtacaggg aataggaccc ctcgtactca aggggaccgt ttccgtccat
     2401 ggggttgccg aggccgttga tgaccctccc caacagtttc ctgcccacct tggcgtcggc
     2461 cctccttccc ctcgccacga tccg
//
LOCUS       DAOTMX010000264         2435 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_33785_length_2435_cov_3.430672, whole genome shotgun sequence.
ACCESSION   DAOTMX010000264 DAOTMX010000000
VERSION     DAOTMX010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33785_length_2435_cov_3.430672"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="P5573_07390"
     CDS             <1..658
                     /locus_tag="P5573_07390"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CDC27 family protein"
                     /protein_id="HRT27922.1"
                     /translation="TGWFPSTKARIWPNDPDIFFEMPVHEVADRSIYLKGYRVVKTDI
                     PVHHYGKLCIDHDSKKGERYLKLLERLIEENENDIRSVEEIATQLQNLKRYEEAIKFW
                     EKYLELKDPAVRPAHQVYNAWLNIGHCLYCLGRVEEGLEASKKAWEANPDSREAACNT
                     AVCHFSLQHYEEALRIASAVVEKHPEYPTAVAVRNAATEMLKRTKQQESDFHLSHPRI
                     "
     gene            721..1740
                     /locus_tag="P5573_07395"
     CDS             721..1740
                     /locus_tag="P5573_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27923.1"
                     /translation="MEHEKNDTPQGQVTTEEQIDMIADHIGNLLDAPEPTKTDEGEST
                     QPTEREVTEVKAGSEPPKAPADDMKADEKPAKEVTAERIKIKWEGEERELTKDELVEL
                     AQKGYDYTRKTQALAERERQLAPLEGIAKTVQSDPAFAAYLRDYFLQKGKPKTEEQPP
                     PKFDDPIEELKYNLKKEIQEEYRKELSEKERAVAHQLVIQRVRNEVQRDPDFQDVTRL
                     MIEHLKTLPEETGRLVFLQWDQNPDAYLKAFSHYKSVVQGLKAKTPPPAPATENTPDA
                     KPPERRTEKAPLLESSGSGEPPDTVAERNKKISKMKAKALRSGDPMAIAEWLLESGSI
                     DNLIQ"
     gene            1751..>2435
                     /locus_tag="P5573_07400"
     CDS             1751..>2435
                     /locus_tag="P5573_07400"
                     /inference="COORDINATES: protein motif:HMM:NF036552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 family protein"
                     /protein_id="HRT27924.1"
                     /translation="MAISTTYAPAAITKSMREDLSDVITNIAPTDTVFMSNIGKQKVK
                     STYHEWLEDTLASAANNAVTEGNDAVLATIVAPTRVGNYTQISAKWFAISDTLEAVDK
                     AGRKSEIAYQTSLRLKELARDMEYALLNNAVATATDPRSLKGVKGFITTNCINFTTGT
                     SVTTLTETRFNDDIQRCWAAGGNPDMVICPAALKRKISAFTGNSKITTNIDAESQKII
                     LSVDYYESDF"
BASE COUNT          706 a          671 c          653 g          405 t
ORIGIN      
        1 tacgggatgg tttccatcta cgaaggcccg gatatggccg aacgaccctg acatcttttt
       61 cgaaatgccg gtccacgaag tagccgaccg ctcaatttac ctgaaaggct atcgcgtagt
      121 caagacagac atcccggtcc accactacgg aaaactgtgc atcgaccacg attccaagaa
      181 gggcgagcgg tatctgaaac tgctcgagcg cctcatcgag gaaaacgaaa acgacatcag
      241 atcggttgag gaaatagcga cccaactcca aaaccttaag cgatatgaag aagccatcaa
      301 gttttgggag aaatacctcg agcttaagga ccccgcggtt aggcccgcac accaggtata
      361 taacgcctgg ctgaatatag gtcattgtct ctactgtctc ggtcgcgtcg aggaaggcct
      421 cgaggcgtcg aagaaagcat gggaggcaaa cccggattcg cgggaagcgg cctgcaatac
      481 ggcggtatgc cacttttcac ttcaacacta tgaggaggca ttacggatag ccagcgcagt
      541 cgttgagaaa caccctgagt atccgacggc cgtcgcggtg cgtaatgccg caacagaaat
      601 gctcaaaaga acaaaacaac aggagtccga tttccattta tcgcatcccc gtatttagta
      661 acacctaaaa accatctaac acggccaacc cttaagggag ccgaggaagg acaaactaca
      721 atggaacacg aaaagaacga caccccacag gggcaagtca cgacagaaga gcagattgac
      781 atgattgccg atcatatcgg taatctgctg gacgcaccag agccgactaa aaccgacgag
      841 ggcgaatcca cacagcccac agaaagggaa gtaaccgagg tcaaagccgg gtctgagccc
      901 ccgaaggcgc cggccgacga catgaaggcc gatgagaagc cggcaaagga agtcacagcg
      961 gaaagaatca aaatcaagtg ggagggtgaa gagagggagc tcaccaaaga cgaattggtc
     1021 gaactcgcac agaagggcta tgactacacc cgaaagaccc aggcacttgc cgaaagggaa
     1081 cgtcagctcg cccccctgga agggattgcc aagaccgtac agagcgaccc cgcatttgcc
     1141 gcttacctac gagattattt ccttcaaaag gggaaaccca agacagagga acagccgccg
     1201 ccgaaattcg acgatccgat tgaagaactt aagtataacc tgaagaagga aatccaggaa
     1261 gaatatcgga aggaactctc ggagaaggaa cgggcggttg ctcatcagct tgtcattcag
     1321 agagtcagaa acgaggtcca gcgggacccc gatttccaag acgtgacaag gctgatgatc
     1381 gaacacctta agacccttcc cgaggaaacg ggcaggcttg ttttcctaca atgggaccaa
     1441 aaccccgacg cctacctgaa ggcgttttct cactataagt cggtagtgca gggccttaag
     1501 gcaaagacgc ccccccctgc gccagcaacg gaaaacacac ccgacgcgaa accgcctgag
     1561 cgaaggacgg aaaaggcccc cctgctcgag tcctcgggct ccggggagcc gccggacaca
     1621 gtcgctgaga gaaacaaaaa aatctcgaag atgaaagcaa aggcattgcg gagcggggac
     1681 ccgatggcga tagccgaatg gctactcgaa agcgggtcaa tagacaatct catccaatag
     1741 ggaggtaaca atggctattt ctacgactta cgcccctgcc gccatcacta aaagtatgcg
     1801 ggaggatctg agcgacgtta ttacgaatat cgcgccgacg gacactgtgt tcatgtccaa
     1861 catcggcaaa cagaaagtga agtccaccta tcacgaatgg ctcgaggaca cgctggcctc
     1921 cgcggctaac aatgccgtga ccgaaggcaa tgacgctgtc ctggcgacca tcgtggctcc
     1981 gaccagggtc ggaaactaca cgcagatttc cgcaaagtgg tttgccattt ccgacaccct
     2041 cgaggccgtt gataaggccg gccgcaagtc cgagatcgcc tatcagacga gcctgcgcct
     2101 gaaggaactg gcccgcgaca tggagtatgc gttgctgaac aacgccgtcg ccacggccac
     2161 ggaccccagg agcctcaaag gtgtaaaggg cttcatcact acgaactgca tcaacttcac
     2221 gaccggaacg tcggttacga cgctgacgga aacgcggttt aacgacgaca tccagcggtg
     2281 ttgggctgca ggcggcaatc ccgatatggt gatctgcccg gcggccctta agcggaagat
     2341 ttccgctttc accggcaact ctaagatcac gacaaacatt gatgccgaga gccagaagat
     2401 catcctctcg gttgactact acgaaagcga tttcg
//
LOCUS       DAOTMX010000265         2433 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_33823_length_2433_cov_3.354920, whole genome shotgun sequence.
ACCESSION   DAOTMX010000265 DAOTMX010000000
VERSION     DAOTMX010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33823_length_2433_cov_3.354920"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="P5573_07405"
     CDS             complement(<1..199)
                     /locus_tag="P5573_07405"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HRT27925.1"
                     /translation="MATTKRVVIARLGSDDRWRGIITITRALRDAGMEVIYLGDQLPE
                     SIVQSAIQEDADVVGLSSFSGN"
     gene            418..1137
                     /locus_tag="P5573_07410"
     CDS             418..1137
                     /locus_tag="P5573_07410"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HRT27926.1"
                     /translation="MYGNKTVMAVFTLLFIFLLSVAAAEAMTPEESLKKNFPDLTYEA
                     FSPSPVKGLYEVTAGMQIYYYAPEAEILIVGQLIAKDGTNQTAARKLKLVAGKIKEIP
                     LEKALKIGNGKNQVIEFSDPDCGYCRKASEFFSKREDVTRYVFFFPLSERSETKVRHI
                     LCSADRERAYKNVYEGKMDGTACGVCKDEKVETIIKADKDAGNRLGIEGTPFFFINGK
                     AVPGADLELIEKLLTGQQPLN"
     gene            1166..2293
                     /locus_tag="P5573_07415"
     CDS             1166..2293
                     /locus_tag="P5573_07415"
                     /inference="COORDINATES: protein motif:HMM:NF014090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HRT27927.1"
                     /translation="MQGEKADLRRFRENLDDERNAAYLYHALAGIEKNPKIASVYRRL
                     AETERGHAEIWAERIRAAGEVIPDFRPSWRTRTVIWLARHFGIHAVLPTLSSVEDGAS
                     SGYGRFPDSRNMVRTERSHATILRHISQTTGGGMEGGILARFEGRHRAAGGNALRAAV
                     LGASDGLVSNFNLVMGVAGAQLSGGSILLTGFAGLLAGSISMALGEWISVQSSRELYE
                     KQLQTEKEEIDSTPEEETEELSLIYQARGLDEDEARRLAARIMSNPSTALDTLAREEL
                     GINPEELGGSAWEAALTSFLLFAVGAVIPVIPYLFLGGSAAIIASASVSGLGLFGIGA
                     AITLFTGRSVWSSGLRQVLFGFAAATVTFTIGRILGVSIAG"
BASE COUNT          551 a          708 c          683 g          491 t
ORIGIN      
        1 ggtttccgga gaacgagctg aggccgacca catcggcgtc ttcctggatg gcagattgca
       61 cgatgctctc cggcagttga tcgcccaggt atatgacctc catgcccgcg tcgcggagtg
      121 cacgggtaat cgttattatt ccccgccaac ggtcatcgct cccgagacgg gctatcacca
      181 cacgttttgt cgttgccatg gctttcacct ctccttaatc gattctgctc ccaccgggcc
      241 ataaaatggt tatttctacc ttatctgccc cgaatcggca agctcgattt gactattcaa
      301 gacgtgcttc gccgacaaaa ggtcggcttc aggtcaagct tcaggtcgag cgaattgact
      361 ttttctggcg tttcctgcaa tataataaaa aatctttccc cccgaagagg ccgaactatg
      421 tacggcaaca aaacagtcat ggcggttttt acgctcttat tcattttttt gctttctgtt
      481 gcagctgcgg aggccatgac accggaggag tcgctgaaga aaaattttcc cgacctgact
      541 tatgaagcct tcagcccctc tcccgtcaaa ggactgtacg aagttaccgc cgggatgcag
      601 atatattact atgcgccgga agcggaaata ctgatcgtag gacagttgat cgcaaaggac
      661 ggcactaatc agacggcagc gcggaagctc aagctcgtcg ccggaaagat aaaggaaatc
      721 cccctcgaaa aggcattgaa aatcggcaac gggaagaatc aggtgatcga gttctccgac
      781 ccggactgcg gctattgccg taaggcctcg gagttctttt ccaagcgtga agacgtcacc
      841 cgttatgtct tctttttccc cctttccgag cggtcggaaa caaaggttcg tcacatcctg
      901 tgctcagccg accgtgaaag ggcttacaag aacgtctacg agggcaaaat ggacggcacc
      961 gcctgtgggg tttgcaagga cgaaaaggtt gagacaatca tcaaggccga caaggatgcc
     1021 ggcaaccggc ttgggatcga aggaacgcct ttcttcttca tcaacggcaa agcggtcccc
     1081 ggcgccgacc tggagcttat cgagaagttg ctgacaggac agcaacccct caactgactc
     1141 ggcgctgacc atgcggaatc gggagatgca gggggaaaaa gcagaccttc gccggttcag
     1201 agaaaacctg gatgatgaaa ggaacgccgc ctacctttac cacgccttgg cgggaatcga
     1261 aaaaaatccc aagatagcgt cggtataccg tcgtctcgcg gaaacggaga gaggccacgc
     1321 cgagatctgg gccgagcgga tcagggccgc gggggaagtg atccccgact tccgcccgtc
     1381 ctggcgcacg aggaccgtca tctggctcgc ccgtcacttc ggcatccatg ctgtgcttcc
     1441 tacgctctct tcggtcgaag acggcgcctc cagcggttac ggtcgttttc ccgattcccg
     1501 gaatatggtc aggaccgaac gatcccatgc gacaatcctg cgacacatca gccaaaccac
     1561 cggcggaggg atggaaggcg ggatactcgc ccgcttcgag ggacggcacc gcgccgcagg
     1621 gggaaacgcc cttcgggcgg cggtactggg ggcgagcgac ggccttgtct cgaatttcaa
     1681 tctggtgatg ggcgtcgccg gggcccaact ctccggcggc agtatcttac tcaccggttt
     1741 tgccggactc ctcgcagggt ccatatcgat ggccctcgga gaatggatat cggtgcaaag
     1801 ctccagggag ctttacgaga aacagctcca gacggagaag gaagaaatcg attcgacccc
     1861 ggaagaagag accgaggagc tgagtctgat ctatcaggcc cgcgggctgg atgaagatga
     1921 ggcacgccga ctcgccgcca ggatcatgag caacccctcg accgcccttg acacattggc
     1981 ccgggaagaa ctggggatca acccggagga gctcggcggc tccgcctggg aggctgcctt
     2041 aacgtccttc ctgctcttcg ccgtcggcgc agtcatcccg gtcataccat acctattcct
     2101 cggcggttcg gctgcgatca tcgcgagcgc ctccgtcagc ggactgggcc tcttcggcat
     2161 cggcgcagcc atcacgcttt tcaccggacg ttcggtctgg tcttccgggc tgcggcaggt
     2221 gctgttcggg tttgccgccg caacagtaac cttcacaatc ggccgcatcc tcggcgtcag
     2281 catagccgga tgagcatttt cccccattca ccctgagcgg gatacaacga acagagacag
     2341 aaagaaacca aaatggatct aaagtttgtc tggaaaatca tcaaagaggc gtctgcaaaa
     2401 gcggtggacg acaacgtacc ccagcttgcc gcc
//
LOCUS       DAOTMX010000266         2418 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_34078_length_2418_cov_3.702920, whole genome shotgun sequence.
ACCESSION   DAOTMX010000266 DAOTMX010000000
VERSION     DAOTMX010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34078_length_2418_cov_3.702920"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..1271)
                     /locus_tag="P5573_07420"
     CDS             complement(27..1271)
                     /locus_tag="P5573_07420"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRT27928.1"
                     /translation="MKDNISGPEIKYIKRRLAQKIREAIKSFPVIVVSGARQVGKSTM
                     LKNEFSDFFYYTMDDFTTLEAAHVDPFSLWKDKDLVIIDEAQKMPGVFNAIKLTVDSD
                     RNRRFIISGSANLLLMEKITESLAGRALYFDLLPMTYGETSGSLFPANFPSLLEVGET
                     KLPKSVDYIDPIPFLMRGFMPPTLTLEEHREVLTWLEGYTKTYLERDLRGLSQVESLI
                     DFRKVMQVLALRTGNVLNQADVAKDSGVSHPTTHRYIRLLEVSNIVQRVGPFFSNRTK
                     RIVKSPKIFFVDPALSVFLAGYYDKESLLKARESGGFFETMVYLHLRSVCETMTPKAV
                     LHYWRLTTGREVDFVVEHGRRLLAIEVKMTDNPTVRDINNLLIFLQEYPETALGILLN
                     SGDAVKWLHSRVVTVPWWWLDL"
     gene            1659..1913
                     /locus_tag="P5573_07425"
     CDS             1659..1913
                     /locus_tag="P5573_07425"
                     /inference="COORDINATES: protein motif:HMM:NF019540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HRT27929.1"
                     /translation="MKKSKLLQKLLSGSKNIRFSEAVACAEMCGFRLERIKGSHHIFV
                     HPDVPELVNLQDVKGEAKQYQVKQLLQLIERYNLPMEEEE"
     gene            1910..2128
                     /locus_tag="P5573_07430"
     CDS             1910..2128
                     /locus_tag="P5573_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HRT27930.1"
                     /translation="MKDYHINVFYSDEDEGYIADIPDLKHCSAFGTTPEEALQEVLKA
                     KEAWLEAARVNGKPIPTPRFRPLIYQVG"
     gene            2121..2402
                     /locus_tag="P5573_07435"
     CDS             2121..2402
                     /locus_tag="P5573_07435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HRT27931.1"
                     /translation="MGKQERLLFLEEILDELGISQAEFARTVGLSPMRVSHVVKGTRP
                     ITAELALLFGKAFGQSPLYWLNLQAAYDLKTVEKAIGRRLAGIPELAPA"
BASE COUNT          538 a          656 c          643 g          581 t
ORIGIN      
        1 gggcggggac gaattcagga ttccgtctac aggtccagcc accaccaggg gacggttacg
       61 acccttgagt gaagccactt taccgcgtcg ccgctgttca agaggatgcc gagcgctgtt
      121 tccgggtatt cctgcaaaaa tatgagcagg ttgtttatat ccctcaccgt ggggttgtcg
      181 gtcatcttta cttcaatcgc cagcagcctc ctgccgtgtt caacgacgaa atcgacctcc
      241 ctgcccgtcg tcaggcgcca gtaatgcaga accgccttcg gggtcatcgt ttcgcagaca
      301 ctgcgcagat gcaggtacac catggtctcg aagaagccgc ccgattccct cgccttcagc
      361 aacgattcct tgtcgtaata accggccagg aatacgctca acgccgggtc gacgaagaat
      421 atcttcggcg atttgacgat tcgtttcgtc ctgttgctga aaaaaggccc gacgcgttgg
      481 actatgttcg acacctcgag cagtcttatg taacgatgag tggtaggatg gcttacgccg
      541 ctgtcttttg ccacgtccgc ctggttcagc acatttcccg tcctcaaggc caggacctgc
      601 atcaccttcc tgaagtctat gagggattcc acctgagaga gccctcgcaa gtccctttca
      661 aggtaggtct ttgtgtaccc ttccagccac gtcagcactt ctctatgctc ttccagtgtc
      721 aatgtgggcg gcatgaagcc tcgcatcagg aagggtatgg ggtctatgta gtcgacggat
      781 ttggggagct tcgtctcccc tacctccaac agggacggga aattcgcggg gaagaggctt
      841 ccagaggtct caccgtaggt catgggcaag aggtcgaaat agagcgccct cccggcgagc
      901 gattcggtga tcttctccat aagcagaagg ttcgcggagc ccgagatgat gaaccgcctg
      961 ttcctgtcgg aatcgaccgt aagttttatg gcattgaaga ccccgggcat cttttgggct
     1021 tcgtcgatga tgacgaggtc tttgtccttc cacagcgaaa aggggtccac gtgagccgcc
     1081 tccagcgtgg tgaaatcgtc catcgtgtag taaaaaaagt cgctgaattc gttcttcagc
     1141 atcgtgcttt tgccgacctg cctggcgccg gagacgacga tgaccggaaa gctctttatg
     1201 gcctccctta tcttttgagc gagacgccgc ttgatgtatt taatttcagg ccctgaaata
     1261 ttatctttca tagcctgagc atattgccca tgattgcgtg atgtcaagta gaatcgaaca
     1321 ccgccgcccc cggcccccct caccctgacc ctctcccgcc aggggcgagg gaatgttcct
     1381 cccccgcctg ttgcctcgcc tttccctccc ccacttgttt cattcttttg ttcctccccc
     1441 acctgtgggg ggaggtcagg aggggggagg cacatgagca tagctgcctt ttgtgaacat
     1501 cgaggcctgc ccgccgcttt gcaataggtt cgatcatgcg atatactcaa cacctaaaca
     1561 tcttcagacc ttctgggttg ccagaggcga tagcccgggc acgtgggttc agtcaccgcc
     1621 cttctgttga tgcctttgtt aggcacacga gaaagattat gaagaaaagc aagctgttac
     1681 aaaagttact ttccggatca aaaaatatcc gtttttcaga agcggtagca tgtgctgaaa
     1741 tgtgtggatt tcgtttggaa cgtatcaaag gtagtcacca cattttcgta cacccggatg
     1801 tcccagaact ggtaaatctc caagatgtta aaggtgaggc aaaacagtat caggtcaaac
     1861 agttgttgca acttattgaa agatataatc ttcctatgga ggaagaggaa tgaaggacta
     1921 tcatatcaat gtattttaca gcgacgagga tgaagggtac attgcagata tacccgactt
     1981 gaagcattgc tctgcttttg gcacaacgcc ggaggaagcg ttacaggaag tgttaaaggc
     2041 aaaggaagcg tggcttgaag cagcgcgtgt aaacggcaaa ccgataccaa cccctcgttt
     2101 ccggccgttg atctatcaag ttgggtaagc aagagcgcct gttgtttctc gaggagatac
     2161 tcgacgaact cggaatttcc caggcggagt ttgcccggac tgtcgggctt tctcccatga
     2221 gggtgtccca tgtcgtcaag gggacccgtc ccatcacggc tgagcttgcc ctgcttttcg
     2281 gcaaggcctt cggacagtcg ccgctgtatt ggctaaacct ccaggcggca tacgacctca
     2341 agacggtcga gaaggccatc ggcaggaggc tcgcagggat accggaactg gcgcccgcct
     2401 gagagttcat catgcccc
//
LOCUS       DAOTMX010000267         2416 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_34116_length_2416_cov_3.268954, whole genome shotgun sequence.
ACCESSION   DAOTMX010000267 DAOTMX010000000
VERSION     DAOTMX010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34116_length_2416_cov_3.268954"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="P5573_07440"
     CDS             complement(<1..687)
                     /locus_tag="P5573_07440"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRT27932.1"
                     /translation="MFEPMEDAAALLKIIHELAAEINPRRARRPVTLDSSLARDVGLD
                     SLARVELLARVEKHFQVTIPERLFAQAETPRDLLQALRSAGPARKAAVEIEVVTEVSE
                     EAPPAPYTARTLVDVVDWHVLNHPDRPHIRFLDEEGEEGVMTYRQLLEGAEAVAAGLQ
                     HHGLLPGKPVSIMLPTGREYFFSFLGILLAGGIPVPIYPPARRSQVEDHLRRHSAILK
                     NCGARIMILVP"
     gene            complement(938..1024)
                     /locus_tag="P5573_07445"
     tRNA            complement(938..1024)
                     /locus_tag="P5573_07445"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(987..989),aa:Leu,seq:gag)
     gene            complement(1109..1456)
                     /gene="secG"
                     /locus_tag="P5573_07450"
     CDS             complement(1109..1456)
                     /gene="secG"
                     /locus_tag="P5573_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HRT27933.1"
                     /translation="MHTFITIVHITVCILMVLVVLLQKGKGASMGAAFGGSSQTIFGS
                     SGPGTFLGKITTAVAVVFMLTSLYLAYFSVHKERSSLMEGAARPGVEQPIGQPAPPQV
                     PTAPSGGTGAQGK"
     gene            complement(1471..2232)
                     /gene="tpiA"
                     /locus_tag="P5573_07455"
     CDS             complement(1471..2232)
                     /gene="tpiA"
                     /locus_tag="P5573_07455"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HRT27934.1"
                     /translation="MKTTSKPLIIGNWKMHKTVPEARDFIRRLEEILPTLTDREVVIA
                     PPFTALLAVAGMTVGTSIKVSAQNLHWEEKGAFTGEVSAAMVADCGCEYVIIGHSERR
                     TLFGETDETVNRKTRTALANGLRPVICVGETLAERESDATLTVVATQIKEGLKGIPFD
                     GIRRVVIAYEPVWAIGTGKTATPGQAQEVHRFIREDIEKAYTKDVADGLPILYGGSVH
                     PDNIDYLMAERDIDGVLVGGASLDAESFARIILFE"
     gene            complement(2233..>2416)
                     /locus_tag="P5573_07460"
     CDS             complement(2233..>2416)
                     /locus_tag="P5573_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HRT27935.1"
                     /translation="EKELVSVDFTSSPYSAVVDAPLTNVLDGNLAKVLAWYDNEMGYA
                     CRMRDLALYIMSRDKE"
BASE COUNT          474 a          714 c          694 g          534 t
ORIGIN      
        1 ggggacgagg atcataatcc gggcgccgca attcttcagg atggcgctgt ggcgccgaag
       61 atggtcttcg acctggctgc ggcgcgccgg cgggtagata ggcaccggga taccgcccgc
      121 gagcaaaatg ccgaggaagg aaaagaagta ctcccgcccc gtgggcagca tgatggaaac
      181 gggctttccc gggagaaggc cgtgatgttg gagacccgcg gcgacggcct ccgcgccctc
      241 aagaagctgc cggtaggtca ttacgccttc ttcaccttct tcatcgagga acctgatatg
      301 gggtctgtcc ggatggttga ggacatgcca gtcgacgacg tcgaccaggg tccgggccgt
      361 ataaggtgcc ggaggggcct cttctgagac ctcggtgacg acttcgattt ccactgccgc
      421 cttccgcgca ggcccggcgc tgcgcagggc ctgcaagagg tcccgcggcg tttccgcttg
      481 ggcgaagagg cgctccggga tcgtgacctg aaaatgcttt tcgacgcggg ctaggagttc
      541 gacccgggca aggctgtcga gaccgacatc ccgggcaagg gagctgtcga gagtcaccgg
      601 ccgccgcgcc ctgcgagggt tgatctcggc ggccagttcg tggattatct tgagcagtgc
      661 ggcggcgtct tccatcggct cgaacatacc ctgttgtggt ttgtctttca agtttcatag
      721 cggtttaatc atcaacgtca ttttacgacc cctacgctca acgccggcga acatccggcc
      781 gggtccggaa gacgatgtgg atgagaatgt gcagatagtc cacgagaggt tgtgtgtcga
      841 ggacagtgat agatgtgaga tattccccaa aaaatacaac ggggattcag acctacctct
      901 gaatccccgt tgtcacggtg ataaatcgct ttacttttgg tgccgaagcc gggacttgaa
      961 cccggacgga cggtgtccac taccccctca agatagcgtg tctaccaagt tccaccactt
     1021 cggcaaatga gatacctcta ttttgaaata tcgtattttc ttttccgtct tttgtcaaca
     1081 agagaaatcg cgtacaaccg ttatttgcct atttgccctg tgccccggtt ccgccgctcg
     1141 gcgccgtcgg gacctgcggc ggtgcgggtt gtccgatcgg ttgctcgacg cccggccttg
     1201 ccgcgccttc catgagggaa gatcgctcct tgtggacgga gaaataagcc aggtacaggg
     1261 acgtcagcat gaatactacc gcgaccgccg tcgtaatctt ccccaggaaa gtccccggcc
     1321 cgctgcttcc gaatatggtc tgactggacc cgccgaaggc ggcccccata cttgcgccct
     1381 tgcccttctg gagaaggacg acgaggacca tcagtataca aacggtgata tggactatag
     1441 tgatgaaggt atgcactttt tataaacacc tcactcaaac aggatgatgc gggcgaacga
     1501 ttccgcgtcc aaacttgccc cgcctaccag aaccccgtca atgtcgcgct cggccatcag
     1561 ataatcgatg ttgtccgggt ggacgctgcc tccgtataag atcggcaggc catcggccac
     1621 gtccttcgtg tacgcctttt cgatgtcctc ccggataaac cggtggacct cttgcgcctg
     1681 ccccggggtt gccgtttttc ccgtaccgat cgcccacacg ggttcgtaag caataacgac
     1741 ccgccttata ccatcaaagg gaataccctt caatccttct tttatctggg tcgctaccac
     1801 cgtcaaggtg gcgtcggatt cccgttctgc gagggtctcg ccgacacaga tgacaggcct
     1861 caacccgttc gcaagggccg tcctggtctt tctgtttacc gtctcgtcgg tttctccgaa
     1921 aagggtccgc cgctccgaat ggccgatgat cacgtattca caaccgcaat ccgccaccat
     1981 ggccgcggaa acctcgcccg tgaaggcccc tttttcttcc cagtgaaggt tctgagctga
     2041 gaccttgatg gatgtgccca ccgtcatccc ggccacagcg aggagggcgg tgaagggagg
     2101 cgcaataacg acctccctgt ccgtcagggt cggcagtatt tcttccaggc ggcggatgaa
     2161 atcccgggcc tccgggacgg tcttgtgcat cttccagttg ccgatgatca ggggcttcga
     2221 tgtcgtcttc acttattcct tatccctgct catgatgtag agggccaggt ctctcatccg
     2281 gcaggcgtac cccatctcgt tgtcgtacca ggccaagacc ttggctaaat tgccgtcgag
     2341 gacgtttgtc aggggtgcgt caacgacagc cgaatagggg ctgctcgtaa agtcgacgga
     2401 gaccagttct ttttcg
//
LOCUS       DAOTMX010000268         2411 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_34217_length_2411_cov_4.075552, whole genome shotgun sequence.
ACCESSION   DAOTMX010000268 DAOTMX010000000
VERSION     DAOTMX010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34217_length_2411_cov_4.075552"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="P5573_07465"
     CDS             <1..330
                     /locus_tag="P5573_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27936.1"
                     /translation="LLKRASELYREDMEKTRFKPFRVPVISNCDPSLFYTEDNAKDLL
                     RRQIVSPVRWLETVERMTSLGIEAVLEIGPKRVLSGLIRNINRDIRLFYVGDAETLRK
                     VASTMAV"
     gene            complement(438..2120)
                     /locus_tag="P5573_07470"
     CDS             complement(438..2120)
                     /locus_tag="P5573_07470"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRT27937.1"
                     /translation="MGLTEIVDKSRFVHLEGNVEEITRGFVRVNMKNLFPDSGLFFDA
                     YFPVADRDYNVVMERILPRGMYCDRRTRQMLADKGFQYFYVRKEDTKAFDDYLQANTK
                     SRLSAPDVTPAEKVEILYGNAEYIVETAFIDGRVEEALPQGAAFAKEAARQFSRSRMT
                     AGELLKIFSKDYGTFTHSIQVSLLGMAFCLYLGYSEKETADFGLGALFHDIGKSEVDD
                     GILNKPSRLTAEEFELIKLHPLKGYESLSKLNLLTQEQLMVVLQHHEDVNGGGYPQGL
                     KSQDIHPYAKITRIVDTYDALTTRRSYKDAFPAAQAFQLMETEMKPLLDAQLLKSFWV
                     FMRIRERSVDRDRHVDLSLRIGCVLHIKLSDKAERLPAKLIGMDAGAYLIVQPQNPAK
                     LFDQVFKGKEVVVRYLSDGAIFGFESRVMDFVMQPVRLLFLTYPSKIQSCEIREHKRY
                     DCLYDAQLLLAGVPCRGVVANISVGGCQFVTEGLDEEQTARIKVEDGVTLSFQPPARA
                     PVESLAGEVRNVTRSNGGRSEIGIRFINLQDDKRNALVDCLSNLAMTERTLS"
BASE COUNT          555 a          689 c          603 g          564 t
ORIGIN      
        1 ctgctgaagc gggcctcgga gctgtacagg gaggacatgg aaaagacaag gttcaagccc
       61 ttccgggtac ccgtcatctc gaactgcgac ccctctctct tttacacgga agataatgcg
      121 aaagacctcc tgagaagaca gatcgtctcc cccgtccgct ggctggaaac agtcgaaagg
      181 atgacttctt tgggaatcga ggctgtcctc gagatcggcc cgaaacgtgt cctttccgga
      241 ctgatcagga atataaacag agatatccgc ctcttttatg tcggcgacgc agagacgctc
      301 cggaaagtgg cctcgaccat ggcggtttga atctttaaga gagcggctgg cttcggtggc
      361 ggacggatac gtccccctgc tccacaaatg acttccgggt gttccctgag ccgtatccca
      421 gggtagtgct tgcccgttca ggagagcgtt ctctcagtca tagccagatt gcttaaacag
      481 tccacaaggg cgttcctttt atcatcctga agattgataa accttatgcc gatctccgaa
      541 cgaccgccgt tcgaccgcgt cacgttgcgt acctccccgg caagcgattc gacaggcgcc
      601 cttgccggag gctgaaaact gagcgtgacg ccgtcttcca ccttgatacg agcagtctgc
      661 tcttcgtcaa gcccttcggt gacgaactgg caaccaccca cgctgatgtt ggcgacaacc
      721 ccacgacacg gtactccggc cagtaagagt tgggcgtcgt agaggcaatc gtaccgcttg
      781 tgttccctga tctcacaaga ctgaattttc gagggatagg tcaagaagag aagccgtacg
      841 ggctgcatca caaagtccat cactctggac tcgaaaccga agatggcgcc gtccgagaga
      901 tagcgaacaa cgacctcttt ccccttgaag acctggtcga agagcttcgc cgggttctgg
      961 ggctggacaa tcagataggc accggcgtcc ataccgatca gctttgccgg gagccgttct
     1021 gccttatctg aaagtttgat gtgcaacaca cagccgatcc tgagggagag gtcgacatgc
     1081 cggtcacggt cgaccgaacg ctcccgaatc ctcatgaaca cccagaaact cttgagtagc
     1141 tgcgcatcca aaagtggctt catctcggtt tccatcagtt ggaatgcctg cgccgcggga
     1201 aaggcgtcct tatacgaccg gcgcgtcgtc aaggcatcat aggtgtctac tattctggtg
     1261 atctttgcat aggggtgtat gtcttgactt ttcagtccct gcgggtatcc gccgccgttc
     1321 acgtcctcat gatgctgcaa caccaccatg agttgctcct gcgtcaggag atttaacttt
     1381 gacaagctct cgtagccctt gaggggatgc agtttaatca actcgaattc ctcggccgtc
     1441 aaccgggacg gtttattgag gatgccgtcg tcgacttcgc tcttaccgat gtcgtggaac
     1501 agggctccca ggccgaaatc cgccgtttcc ttctccgagt acccaagata gaggcagaag
     1561 gccataccga gaaggctcac ttggatggaa tgagtgaatg taccgtagtc cttcgagaaa
     1621 atcttcagca actctcccgc tgtcatccgg ctccgcgaaa attgccttgc cgcctccttc
     1681 gcgaaggctg caccctgcgg caaggcctct tccacccgac cgtcaatgaa ggccgtctcg
     1741 actatatatt cggcgttacc atagaggatc tctaccttct cagccggagt tacatcaggg
     1801 gccgacagac ggctcttcgt gtttgcctgc aaatagtcgt cgaaggcctt cgtatcttcc
     1861 ttgcgcacgt aaaaatactg aaagcccttg tccgccagca tttgtctcgt tcggcggtcg
     1921 caatacatcc cgcggggaag tatcctctcc attacaacat tgtaatcacg gtcggcaacc
     1981 gggaaatagg cgtcaaaaaa gagccccgag tcgggaaaga ggttcttcat gttgacccgg
     2041 acaaatcccc ttgtgatttc ctctacattt ccttctaaat gcacgaacct gcttttgtcg
     2101 acgatctcag tcaaacccat aggtcagcta cctctcaaaa atgatcagcg cgaaattctg
     2161 gaacaaacct gtcatttcct tctatcgtca tcatgctcac attctttagt acctgaggac
     2221 gggcgtattt cctgacctct atcctcccca aaaaaagatt aaagctccgt cagcgacagg
     2281 ccgataattg agagggaatg tactgattca ttgataaaaa ctcccaagac gtcatgccgg
     2341 ccttaaccca acgtccggta ttttcaagcg gttcttggtt ccgacttcca tcgcaatgac
     2401 atgaggggtc g
//
LOCUS       DAOTMX010000269         2398 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_34483_length_2398_cov_3.379428, whole genome shotgun sequence.
ACCESSION   DAOTMX010000269 DAOTMX010000000
VERSION     DAOTMX010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34483_length_2398_cov_3.379428"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..923
                     /locus_tag="P5573_07475"
     CDS             <1..923
                     /locus_tag="P5573_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HRT27938.1"
                     /translation="LARWTSGRSREVAMVTREEDFVENIFIASTHSYILIFTNLGRVY
                     WLKVYQVPQAGRAAKGKAIVNLIQISPNERVSAVLPVRDFVGGKYVVMATKRGIVKKT
                     DLTAFSNPRTGGIIAVSVDEGDELIEVKETTGTQDILLGTRKGMAIRFKEDEVRSMGR
                     TARGVTGIRIDEDDEVVGMEVIGNENTILTVSEKGFGKRTEIAEYRLQSRGGKGVINL
                     KTTPKTGAVAGIKQVVGNEDIMLISNTGNIIRLHVDEISVLHRNTQGVKLIDIAPEEK
                     VVGVARTERGNGKDNGKEEEGATAESGEEL"
     gene            920..1336
                     /locus_tag="P5573_07480"
     CDS             920..1336
                     /locus_tag="P5573_07480"
                     /inference="COORDINATES: protein motif:HMM:NF014058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HRT27939.1"
                     /translation="MKPYCLIDHTADLGLEVFGKTGEELFANAASAVCELMLDIENPE
                     PTAVREISVEGTDREDLLVNFLREVLFLFNGEKMVPTSFIIEELDDRHLRGKVRVAAY
                     DPARHRVKREVKAVTYHGTEVVETPTGWRARVIFDV"
     gene            1329..>2398
                     /locus_tag="P5573_07485"
     CDS             1329..>2398
                     /locus_tag="P5573_07485"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HRT27940.1"
                     /translation="MSEIRLQKLDDYRWLIPPTGGMRVPGLIFSSEKMMEEAGKDESP
                     RQVANVACLPGILRYSFAMPDMHWGYGFPIGGVAAFDLKDGVISPGGVGYDINCGCRL
                     VATKLRFEEIRERLQDLVVALFRNIPSGVGSTGPLKLSTEEEKDVLRYGARWAVKAGF
                     GTEADLETTEDGGRIGGADPERVSKRALERGREQLGTLGSGNHFLEVEVVEEVFDEEA
                     AAAFGLEVGQVAVLIHSGSRGLGYQVCDDYLALLVKGIPSLGFPLPDRQLACAYLDSQ
                     EGRDYFAAMACAANYAWANRQMLMHWTREAFEKVLHKSPVELGMRLVYDVCHNIAKFE
                     EFPLEGGDVKVCVHRKGATRSFP"
BASE COUNT          520 a          645 c          820 g          413 t
ORIGIN      
        1 tgctggctcg atggacctca ggtcgctcga gagaggtggc gatggtgacg agggaagagg
       61 acttcgtgga gaatatcttc atcgcctcga cccacagcta catcctgatc ttcacgaacc
      121 tcggccgcgt ttactggctc aaggtgtacc aggtgcccca ggcgggcagg gccgccaagg
      181 ggaaggccat cgtgaacctg atccagatat cgccgaacga acgcgtctcc gccgtcctgc
      241 cggtccgtga cttcgtgggc ggcaagtacg tcgtcatggc gacgaaaagg gggatcgtca
      301 aaaagacgga cctcaccgcc ttctcgaacc cgaggacagg ggggataatc gcggtcagcg
      361 tcgacgaagg ggatgagttg atcgaagtca aggagacgac aggtacccag gatatactgc
      421 tcggcacgcg caaaggtatg gccatccggt ttaaggaaga cgaggtaagg tccatgggca
      481 ggacggccag gggcgtcacg ggtatccgga tcgacgaaga cgacgaggtc gtgggaatgg
      541 aggtcatcgg aaacgaaaac accatcctga cggtttcgga aaagggtttc ggcaaacgca
      601 cggagattgc cgaatacagg ctccagtccc ggggcgggaa aggcgtcatc aacctgaaga
      661 ccaccccgaa gacaggcgcc gtggccggga tcaagcaggt cgtcggtaac gaggacatca
      721 tgctcatcag caacacgggc aacatcatcc ggctgcacgt cgacgagatc agcgtcctgc
      781 accgcaacac ccagggcgtg aagctcatcg acatcgcacc ggaagaaaaa gtcgtcggag
      841 tcgcgcggac ggaacggggc aacggcaagg acaacggaaa agaagaagag ggcgcgacgg
      901 cggaaagcgg tgaagagttg tgaagcccta ttgcctgatc gaccacacgg cggacctggg
      961 cctcgaggtc ttcgggaaga ccggtgaaga gctcttcgcc aacgccgcct cggcggtctg
     1021 cgagctcatg ttggacattg aaaaccctga accgacggcc gtcagggaaa tctcggtgga
     1081 gggcacggac cgggaagacc tcctggttaa tttcctgcgc gaggtgctgt ttctttttaa
     1141 cggagaaaag atggttccca cctccttcat catagaagaa ctggatgacc ggcatctgag
     1201 gggaaaggtc cgggtggcgg cgtacgaccc ggcgcggcac cgggtcaaga gggaggtcaa
     1261 ggcggtgact taccatggga cggaggttgt cgagacgccg acgggatggc gggcaagggt
     1321 gattttcgat gtctgagata aggcttcaga aactggacga ttatcgttgg ctgatcccgc
     1381 cgacgggcgg gatgagggtg cccgggctga tcttttcgag cgagaagatg atggaagagg
     1441 cgggtaagga cgaaagcccc cgtcaggtgg ccaacgtcgc ctgcctaccc ggtatcctga
     1501 ggtattcttt cgccatgccc gacatgcact ggggctatgg ttttccgatc ggcggcgtcg
     1561 cggccttcga cctcaaggat ggtgttatat ctcccggcgg cgtgggctac gacatcaact
     1621 gcggctgccg cctcgtggcg acgaagctcc gtttcgaaga gataagggag cggttgcagg
     1681 acctggtagt cgccctattc cggaacatcc cctccggtgt gggttccaca gggcctctta
     1741 agctgtcgac ggaagaggaa aaggatgtgc tccggtacgg cgcccggtgg gccgtgaagg
     1801 cggggttcgg cacggaggcc gacctggaga cgacggaaga cggcggcagg ataggagggg
     1861 ccgacccgga acgggtaagc aagagggccc tcgagagggg ccgggaacag ctcggaacgc
     1921 tggggtcggg caatcacttc ctcgaggtgg aggtggtcga agaggtcttt gacgaggagg
     1981 cggctgccgc cttcggcctg gaggtaggcc aggtcgccgt gttgatccac tcggggtcgc
     2041 gggggttggg ctaccaggta tgcgacgatt acctggcttt gcttgtgaag gggattcctt
     2101 cccttgggtt tcccctccct gaccggcaac tcgcgtgcgc ctacctcgac tcacaggagg
     2161 gccgggatta tttcgccgcc atggcctgcg ccgccaacta cgcctgggcc aaccgccaga
     2221 tgctcatgca ctggacccgg gaggccttcg aaaaggtgct gcacaaaagt cctgtcgaac
     2281 tggggatgag actggtctac gatgtctgtc acaacatagc gaaattcgaa gagtttcctc
     2341 tcgaaggggg cgacgtcaag gtctgcgtcc accgcaaggg ggcgacgcgc tccttccc
//
LOCUS       DAOTMX010000270         2397 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_34503_length_2397_cov_3.570453, whole genome shotgun sequence.
ACCESSION   DAOTMX010000270 DAOTMX010000000
VERSION     DAOTMX010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34503_length_2397_cov_3.570453"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /gene="rapZ"
                     /locus_tag="P5573_07490"
     CDS             <1..613
                     /gene="rapZ"
                     /locus_tag="P5573_07490"
                     /inference="COORDINATES: protein motif:HMM:NF015620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HRT27941.1"
                     /translation="IEILFLDSSDEVLIHRFKETRRSHPLAEKGTVVEGIALEREKLA
                     SLRSMADKIIDTSPLNVHQLKDVIQRYCMTSPSDRKLVINLTSFGYRYGVPPDADIVL
                     DVRFLPNPYFVEDLKNLDGNDKKVEDYVMGWQESRTFLDELFKLFEFLIPLYEKEGKS
                     YLNVALGCTGGKHRSVVMLSRLGRFLKDKNYLVNLSHRDIYRH"
     gene            complement(610..1941)
                     /locus_tag="P5573_07495"
     CDS             complement(610..1941)
                     /locus_tag="P5573_07495"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRT27942.1"
                     /translation="MKIFTARFWAYLSVVLLVVISLVLFTPYFDVFKPDVRFVGPVDR
                     VGQVKTVTINVSDPDSGLRHVGVSIAQHGIAYTLASLDFPEQGTNHKSFTVELNPRSL
                     KFRDGEALITVTASDRSLFGNTARLEQRVIIDQVPPQISLLSSYHNISPGGSCLAVYS
                     ISKETEKSGIKVGDDFFPGYPAASGDSKVYLAFFPVPLNPDKGSLRITVHAKDTAGNE
                     STASVPYHLKPKRFRSDSVEVSDAFLSKVVLEFQQQDPGLRGKSPVDTFVYINETLRE
                     ENSKAIQAVCAKTASVRLWQGPFLRMKEAAPMALFGDRRTYTHHRQKIGESIHLGVDL
                     ASTAHAPIEAANRGVIAFSGQLGIYGNAVIIDHGLGIFSLYAHMSELKAVEGREVAKG
                     DVIGTSGETGFAGGDHLHFSILVGGRFVDPVEWWDPHWITDNVEKKLPQVS"
     gene            2184..>2397
                     /locus_tag="P5573_07500"
     CDS             2184..>2397
                     /locus_tag="P5573_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HRT27943.1"
                     /translation="MESLAGIALSSFMIALSGAVMPGPVLTVTISESLRRGFLAGPLI
                     ILGHGLLEIALLAVFVLGFADVIGHPK"
BASE COUNT          539 a          620 c          677 g          561 t
ORIGIN      
        1 gatcgagatc ctcttcctcg actcttccga cgaagtcctg atacaccggt tcaaagaaac
       61 gaggaggagt catcccctgg ccgaaaaagg aacggtcgtt gagggtatcg ctctcgagcg
      121 ggaaaaattg gcgtccctgc gaagcatggc cgataaaatc atagacacct cgccgttgaa
      181 tgtccaccag ttgaaggacg tgattcagag gtattgcatg acctcccctt cggacaggaa
      241 gctggtgatc aacttgacct ctttcggcta caggtatggg gtaccccctg acgcggacat
      301 cgttctcgac gtgaggtttc tccccaatcc ctattttgtc gaagacctga aaaacctgga
      361 cggcaacgac aagaaggtgg aggattacgt catgggatgg caagaaagcc gtacgttcct
      421 tgatgaactt ttcaaactct tcgagttctt gattcccctc tacgaaaaag aagggaaatc
      481 ctacctgaat gtcgccctcg ggtgtaccgg cggcaagcac cgttccgtcg tgatgctttc
      541 acggctgggg cggtttctga aagataaaaa ctacctggtc aatctcagtc atcgcgatat
      601 ctaccggcat taagagacct gcggaagttt cttctccacg ttgtcggtga tccagtgggg
      661 atcccaccac tccacggggt cgacgaagcg accgccgacc aggatgctga aatggagatg
      721 atcgccccca gcaaagcccg tctcaccact cgtgccgatc acgtcgcctt ttgcaacctc
      781 ccgtccttcc accgccttca actcgctcat atgagcgtaa aggctgaaaa taccgaggcc
      841 gtggtcgatg atgacggcat tcccatagat gccgagttgc ccagagaagg cgatgacgcc
      901 tctgtttgcg gcttcgatcg gtgcatgggc cgtcgaagcc aggtcgacgc cgaggtggat
      961 gctttcccct attttctgtc tatggtgggt ataggttcgc ctatcgccga aaagcgccat
     1021 gggtgctgct tccttcatcc gcaagaaagg accctgccag agtctgacgg aggcggtttt
     1081 agcacagact gcctgaatcg ccttggagtt ctcttctctc agtgtttcat tgatatagac
     1141 gaacgtgtcg accggcgatt tgcccctcag gccggggtcc tgctgctgga actcgagcac
     1201 aactttagac aggaaagcgt cggagacctc cacactgtcg ctcctgaatc tcttcggttt
     1261 gagatggtaa gggaccgacg ccgtagactc attacccgcc gtgtctttcg cgtgaactgt
     1321 gatacgtagc gagcccttat ccgggtttag gggtaccgga aagaacgcca ggtagacttt
     1381 actatcaccg cttgcggcag gatagccggg gaagaagtca tctccgacct ttatcccgct
     1441 cttttcggtc tctttcgaga tactgtagac ggcaagacat gagcctcccg gggagatgtt
     1501 gtggtatgag ctcaggagcg aaatctgggg cggcacctgg tcgatgatga ccctttgctc
     1561 gagacgggcc gtgttgccga acaaagaacg gtccgaggcg gtcaccgtga taagtgcctc
     1621 gccgtcgcga aatttgaggc tcctggggtt gagttcgacc gtgaaagatt tatgattagt
     1681 cccttgctcg gggaaatcga gcgaggcgag cgtataggct atcccgtgtt gggcgatact
     1741 gactccgacg tgacgaagtc cgctgtccgg gtccgacacg tttatggtta cggttttgac
     1801 ctggccgacc cggtcgacgg gaccgacgaa gcggacatcc ggcttgaaga cgtcaaaata
     1861 gggcgtgaac aggacaagag atatgacgac aaggaggacg acagatagat aagcccagaa
     1921 ccgggccgtg aaaattttca tatcattcct ttctttgcgg cgatgatcga gatggccttc
     1981 cgagggataa gcaggccggc tccgggcgaa tgggtactaa aagaagagaa atatttcaag
     2041 aaaaaaagag ggatgactcg gttgactgaa cgggttgtac gggcctgaag ggagtttaat
     2101 gccttgacct tgtttgggtt ccctgttata taatcattct taaataccgt tgaaaaatca
     2161 ttcatttttg ggaaagcggt ccgttggaaa gtctggccgg cattgctctt tcctcattca
     2221 tgatagccct gtcgggtgct gtcatgccgg gcccggtcct gaccgtgacg atcagcgaaa
     2281 gcctcagaag ggggtttctc gccggaccgt tgatcatcct tggtcacggt cttctggaaa
     2341 tagccctgct tgcggtcttc gtgctggggt ttgccgatgt catcggacat cccaagg
//
LOCUS       DAOTMX010000271         2394 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_34554_length_2394_cov_4.198375, whole genome shotgun sequence.
ACCESSION   DAOTMX010000271 DAOTMX010000000
VERSION     DAOTMX010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34554_length_2394_cov_4.198375"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="P5573_07505"
     CDS             complement(<1..747)
                     /locus_tag="P5573_07505"
                     /inference="COORDINATES: protein motif:HMM:NF019976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HRT27944.1"
                     /translation="MATMIPSDVEQFETEGERQFYRFLERVARPDNRYLSWYLPDIRG
                     KEPDFLLFSDEVGLVIFEVKDWALNQVREADPQYFILDMGGKEEKHRNPYQQARKYFV
                     DVMKRIKEDGYLLSSDPHFKGKVKIPVNCGVVFPNINKYEYKEKDLHRVIDPDKIFFC
                     DDLHPQSDHWADPSGRCFLEALKRMFTPQFSFHVTGRELERLKGVLFPVVRLELPDRG
                     GKEKREEDRRRLKVLDHNQEALARKIEGGHR"
     gene            complement(1169..1873)
                     /locus_tag="P5573_07510"
     CDS             complement(1169..1873)
                     /locus_tag="P5573_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27945.1"
                     /translation="MMKSSTVMAINYDYRPETYREVKDPVAKALYNVKGTVRRYMIVS
                     ALRDGIYDRLPREIRKETLSDPERQMWGRIHPVAMGGEYLPDYREKEVEIARVELKST
                     TFDVISIRAREKGLFIDYRIVDEYGSAFEFSPKTSSKPLTLRELIELIDGARQEGYED
                     RGLGLCYILHNYEEGQDAEELLDFTVINSIFYPGLSEHYAHVAKQWCEGKTTAKKKKM
                     TGVIICPGGPNRVKEK"
     gene            complement(1870..2172)
                     /locus_tag="P5573_07515"
     CDS             complement(1870..2172)
                     /locus_tag="P5573_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27946.1"
                     /translation="MSEERKREAFRNAANSIRIDREFREYCLEADIGLSYPTNWDDPL
                     ELLGYIDLLGLDENQKVIVRNWTLGLIERQGPLYVWECKLWLKCELKYILEELGGI"
BASE COUNT          509 a          665 c          590 g          630 t
ORIGIN      
        1 gcggtggccg ccctcgatct tgcgggccag ggcctcctgg ttgtggtcga ggaccttgag
       61 gcgccgcctg tcctcttcgc gcttctcttt gccgccccgg tcgggcagct cgaggcgcac
      121 gacggggaag agcacgccct tgaggcgctc gagttctctg ccggtgacgt ggaagctgaa
      181 ctggggcgtg aacatccgct tgagggcctc gagaaagcag cggcccgagg ggtcggccca
      241 gtggtccgac tggggatgca ggtcgtcgca gaagaagatc ttgtcggggt cgatgaccct
      301 gtggaggtct ttttctttgt attcgtactt gttgatgttg gggaagacga cgccgcagtt
      361 cacggggatc ttcaccttac ccttgaagtg ggggtcggag gagagaagat agccatcctc
      421 tttgatgcgt ttcatgacat cgacgaagta cttccgcgcc tgttggtagg ggttgcggtg
      481 tttttcttcc tttccgccca tatccaggat gaagtactgc gggtcggctt cgcggacctg
      541 gttcagtgcc cagtccttga cctcgaagat gacgagacct acctcgtcgg agaacagaag
      601 gaagtcgggc tctttgcccc tgatgtcggg aaggtaccag gagaggtagc ggttgtcagg
      661 ccgggcgacg cgctcgagga agcggtagaa ctgccgctcc ccttcggtct cgaattgttc
      721 aacatcgctg gggatcatcg tcgccatgtc gattcacacc catcagaaaa gaaagcctga
      781 gtcaggagtt gtcatctctt cagccagact aaattcccct ctcccacaac tgcaagccac
      841 gtcttttcct tttcctatca cagatcgctc agcttgactg ataagtatat atatttacag
      901 aagatttttt atttaaaata cgctccttta cggtttgaga tttaagtaaa attcgttcct
      961 ttttctgatt taagtcatta aaactcttat ttatttgact ggggcaagag tgcgcgaacg
     1021 gtcgctaagt aatgaaacaa ggcaagccga tttgaagtga gatcccccct tgtgtaaccg
     1081 atgttacaaa tacccacgat ctttataaat atcaagaact tttttaattt tccctcgatt
     1141 tattgaaaaa cacggggtta gagagtggtt acttctcctt aactctattc ggcccgcccg
     1201 ggcatatgat gacaccggtc attttctttt tctttgccgt tgtcttcccc tcgcaccact
     1261 gcttcgccac atgggcatag tgctccgaaa ggccggggta aaaaatcgaa ttaatgacgg
     1321 tgaaatccag caattcttca gcgtcctggc cctcctcata attatgaagg atgtagcaaa
     1381 gccccagccc ccggtcctcg tagccctcct gccttgcccc gtctatgagc tctatcagct
     1441 cccgcagcgt gagaggcttc gaactcgttt tgggagaaaa ctcgaaggcg ctcccgtact
     1501 cgtcgacgat ccggtagtcg atgaagaggc ccttctcgcg ggcgcggatg ctgatcacgt
     1561 cgaaggtggt agatttaagc tccaccctgg cgatctcaac ctccttttcc ctgtaatcgg
     1621 gcagatattc cccgcccatg gcaacggggt ggattctgcc ccacatttgc ctttcgggat
     1681 cggacagtgt ttccttcctg atttccctcg gcagcctgtc gtagataccg tccctcaaag
     1741 cactgacaat catatacctg cggacggtcc ccttgacgtt ataaagagcc ttcgcaacgg
     1801 gatcctttac ttcccggtag gtttccggac gataatcgta gttgatggcc ataacggtgc
     1861 tactcttcat catatacctc ccaattcctc taaaatgtat ttgagttcgc atttaagcca
     1921 taacttgcat tcccacacgt acagaggccc ctgacgttca atcaacccca gagtccaatt
     1981 cctgacgatt accttctggt tctcgtccag acccagcagg tcaatgtaac cgaggagttc
     2041 cagcgggtca tcccagtttg tggggtaaga caggccaatg tccgcttcca agcagtattc
     2101 cctgaattcc ctgtctattc ggatactgtt tgcagcgttc ctgaaagctt cccgcttcct
     2161 ttcctcgctc atggttccgt cctgtctttt ttgttctttc cgctttcttg aattcccctg
     2221 atgctccatt ttgcattccc ctttccgcca atgaaaaacc ccgcagccaa acaaacgtga
     2281 ccgcggggtc atcggctgtt tagcaccttg ttgaggcggc tgcaagttgc ccgtaaatca
     2341 acccgccaaa aaattgtcaa aaaacaaaaa gcccgcgcta aaagcacggg cccc
//
LOCUS       DAOTMX010000272         2362 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_35172_length_2362_cov_3.770698, whole genome shotgun sequence.
ACCESSION   DAOTMX010000272 DAOTMX010000000
VERSION     DAOTMX010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35172_length_2362_cov_3.770698"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="P5573_07520"
     CDS             <1..380
                     /locus_tag="P5573_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRT27947.1"
                     /translation="TVPKVTVVTRKDYGGAYIGMCSKYLGADYVMAWPSAEIAVMGAE
                     GACNIVYRREIASAEDPAAKRKELVAAYEAQFNNPYFAASMGIVDEIIAPRETRKKVI
                     AFLEAYKDKAEVRLQKKHNNIPL"
     gene            complement(432..1913)
                     /gene="gltX"
                     /locus_tag="P5573_07525"
     CDS             complement(432..1913)
                     /gene="gltX"
                     /locus_tag="P5573_07525"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HRT27948.1"
                     /translation="MKRDKRVRVRFAPSPTGDLHVGNARTALLNWLFARHHGGDFILR
                     VEDTDRVRTSDVYERNILEDLMWLGIDWDEGPEKDGPFGPYRQSGRLEIYRGYLERLV
                     AEGRAYRCYCTEEELEKERAGLLSRGKAPRYGGRCRNLTVEQGKKFDDEGRAYAYRFR
                     VEEGLIEFTDLIRGPMKFNAEDIGDFIVLRSNAVPAYNFAVVIDDYLMKVSHVIRGED
                     HLSNTAAQLMLYAAFGFTPPVFAHHALILGKDRAKLSKRHGSVTVREFRRQGILPEAM
                     VNYLALMGSSYGEGREVMTLAEIVNEFSLDRIGKGGAVFDEEKLDWLNTMYIRKLDLG
                     GLTARVIPFLEEAGLDVRSRPDEWVEAVLGVVKGNLKRLSDAPYQVEIFFDEHFKVSV
                     EAASLLRENESGAVLAALQEALTDGNCPERGFYAFAIDFVRGRTGLKGKAIFTPIRAA
                     VTGRLEGPELSRVFDILDREAILKRVARALASAGASGTKCGCA"
     gene            complement(1910..>2362)
                     /gene="ispF"
                     /locus_tag="P5573_07530"
     CDS             complement(1910..>2362)
                     /gene="ispF"
                     /locus_tag="P5573_07530"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HRT27949.1"
                     /translation="DSHRLATGRPLILGGVEVPFEKGLLGHSDADALLHAVGEAILGA
                     AGERDIGCHFPDSDPAFKDISSRVFLDRIFGLVRKNGFAVNNVDVTVVLEKPKLSPHI
                     PDMRKTLESLLDLPADRVSIKARTNEGMGFVGRGEGVAVFAVVSLKEL"
BASE COUNT          510 a          746 c          618 g          488 t
ORIGIN      
        1 ccaccgtccc caaggtcacc gtcgtgacgc gcaaagacta cggcggcgcc tacatcggca
       61 tgtgcagcaa gtacctgggg gctgattacg tcatggcctg gccgtcggcg gagatagccg
      121 tcatgggcgc ggaaggcgcg tgcaacatcg tctaccgccg cgagatcgcc tccgctgaag
      181 acccggcagc caagaggaag gagctggtcg ccgcttacga agcacagttc aacaacccct
      241 atttcgccgc cagcatgggg atcgtcgacg aaatcatcgc tccaagggaa acgagaaaga
      301 aagtcatcgc cttcttagaa gcttacaaag acaaagcgga ggtcaggctc cagaagaagc
      361 acaacaacat accgctctga cctaaggggc gaaagggcgg tacttaatca ccgccctttc
      421 gcctatcatt attacgcgca gccacacttt gttccggatg cgccggcgct tgcgagggcc
      481 cttgccaccc tcttgagtat ggcttctcta tccaggatgt cgaaaaccct gctgagttcc
      541 ggcccttcga gccgtcctgt gacggctgcc cggataggcg taaaaatggc ctttcccttc
      601 agacccgtcc ttcctctcac gaaatcgatc gcgaaggcat aaaaaccacg ttccggacag
      661 ttgccgtccg tcagtgcttc ctgaagggcg gcgagaaccg cccctgattc attctccctg
      721 agcaacgacg cggcttcgac tgagaccttg aaatgctcat cgaaaaatat ctcgacttga
      781 tacggtgcgt cagaaaggcg tttcaggttt cccttcacca cccccagtac cgcctccacc
      841 cattcatcgg ggcgcgaccg gacgtccaag ccggcctcct ccaggaaggg aatgaccctc
      901 gcggtaaggc ctcccaagtc caattttcgg atatacatgg tattcagcca gtcgagcttt
      961 tcctcgtcga agacggctcc acccttcccg atacggtcga gcgaaaactc attcacgatt
     1021 tccgccaacg tcatgacctc gcggccctca ccgtacgagc tccccataag ggcgagataa
     1081 ttgaccatgg cttccggtaa gatgccctga cgcctaaatt cgcgaacggt cacggagcca
     1141 tgccgcttgc ttagtttggc ccggtccttc cccaggatca acgcatggtg ggcgaaaacc
     1201 ggcggcgtaa aaccgaaggc agcgtaaagc atcagttggg cggctgtgtt cgacagatgg
     1261 tcttctccgc ggataacatg ggaaaccttc atcagatagt cgtcgatgac aacggcaaaa
     1321 ttataggcgg gtacggcgtt cgaacggagg acgatgaaat ccccgatatc ctcggcgttg
     1381 aacttcatcg ggcctcggat gaggtcggtg aattcgatca gaccctcctc cactcggaag
     1441 cggtacgcat atgccctgcc ttcatcgtca aacttcttcc cctgctccac cgtgagattc
     1501 ctgcatcgac caccgtaccg cggggctttt cctcgcgaga gaagtccggc acgttctttt
     1561 tccagctcct cttccgtgca gtaacagcga taggcccttc cttccgcgac gaggcgctcg
     1621 aggtagcccc tgtagatttc cagacgcccc gactggcggt aaggcccgaa agggccgtct
     1681 ttttcaggac cctcatccca gtcgatcccc agccacatca ggtcctcgag gatattcctc
     1741 tcatagacat ccgacgtcct cactctgtcg gtatcctcaa cccgcaggat gaaatcacca
     1801 ccatgatggc gggcgaagag ccagttcaag agcgccgtcc ttgcattgcc cacatgcagg
     1861 tctcccgtcg gcgacggggc gaatcgtacc ctaaccctct tgtctctttt catagctcct
     1921 tcagtgaaac cacggcgaaa acagcgacac cttctccgcg tcctacaaac cccatacctt
     1981 cgtttgtcct ggcctttatg ctgaccctgt ccgccggaag gtcgagcagg gattccagtg
     2041 tctttctcat atccgggatg tggggcgaga gtttcggttt ctctaaaacg acagtgacat
     2101 cgacattatt cacggcgaaa ccgtttttcc tcacgaggcc gaagattctg tccaggaaca
     2161 cccgactcga aatgtccttg aaagccgggt cgctgtcggg aaaatggcac cctatgtcac
     2221 gctccccggc ggcgccgagg atggcttctc ccactgcatg gaggagggcg tcggcgtcgg
     2281 agtgaccaag gagtcccttc tcgaaaggga cctccacccc gccgaggatc aggggtctgc
     2341 ccgtcgcaag gcggtggctg tc
//
LOCUS       DAOTMX010000273         2357 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_35278_length_2357_cov_3.412685, whole genome shotgun sequence.
ACCESSION   DAOTMX010000273 DAOTMX010000000
VERSION     DAOTMX010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35278_length_2357_cov_3.412685"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="P5573_07535"
     CDS             <1..518
                     /locus_tag="P5573_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRT27950.1"
                     /translation="VAKDHHILAFITDTLRTVITTQIEPYEMDALMDMQLEEHHEELM
                     IPSRSLATIADALPGLGIVAAVLGVVISMQKIGESPEVLGKSVGAALVGTFLGVLLCY
                     GFVGPASRLLEHIATAKVQYMTVVKVVLKSFVSNNASPQVAIEFGRRLIPAKVRPDFY
                     EVEEAIRRVKK"
     gene            518..1351
                     /locus_tag="P5573_07540"
     CDS             518..1351
                     /locus_tag="P5573_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRT27951.1"
                     /translation="MADEPKRIIIKKVKKKGHQAAHGGSWKVAYADFVTALMALFLLL
                     WLLAMISPEKRILLSQYFKHFSVFKESGQSVIQGHSSLLDRDGGVAAKRMLGSRESDI
                     SPEGLQERIKGAIAEKLSSLKDQVIVEVLEDDVKIQIVDTEGSLMFKLGSAQPTEKAR
                     QLLQLLAENIRDTANRIVIEGHTDSLPYRGAQITNWELSTARASAARRELEANGIEPS
                     RIARVVGYADQELLIKDEPTNPRNRRISIILLKEKASPPADAVGPDAQNPAEMTGLAI
                     K"
     gene            complement(1380..>2357)
                     /locus_tag="P5573_07545"
     CDS             complement(1380..>2357)
                     /locus_tag="P5573_07545"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRT27952.1"
                     /translation="IFEEMADTALKVSQGESCSLLLFDEEKSEFRPKVVKNLYSEHTL
                     DLQSPSLLSILRKVIQEREACIINSFSGHEGPSSLILAPLRIRNRVFGVLGISRRKNG
                     VEFTPTVLNYILSLTKRASLNIENNILYESTYANLMDTLKSMAASIQARDHYTESHSV
                     RVTELAVRICEAMGSRPEDIESMKIVGILHDIGKIAIPDDILLKPGRLTEAEYDIIKQ
                     HPLTGENIIKPIMLFDRERLVIRHHHERWDGRGYPDGLAGEDIPLLSRIIAVADSFDA
                     MTNNRPYRKAMEVETAVEELRKNAGLQFDREVVHCFLTINEKAGHESLQ"
BASE COUNT          515 a          626 c          696 g          520 t
ORIGIN      
        1 gcgtagcgaa ggaccaccac atccttgcgt tcatcaccga tacgctccgg accgtcatca
       61 ccacccagat cgagccttat gaaatggacg cgctgatgga tatgcagttg gaagagcacc
      121 atgaggaatt gatgatcccc tcgcggagcc tggccaccat cgccgatgcg ctcccggggt
      181 tgggcatcgt cgccgccgtg ctgggtgtag tgatctcgat gcagaagatc ggggagtcgc
      241 cggaggtcct cggaaaaagc gtcggtgccg cgcttgtggg cactttcctg ggggttctcc
      301 tctgttacgg gttcgtcggg cctgcctccc ggttgctcga gcatatcgcg accgccaagg
      361 ttcagtacat gaccgtggtg aaggtcgtcc tgaaatcgtt cgtcagcaat aacgcctcac
      421 cgcaggtcgc catcgagttc ggtcgacggc tcatcccggc aaaggtgcgc cctgattttt
      481 atgaggtaga ggaggcgatc aggcgggtga agaaataatg gccgatgaac ctaaacgcat
      541 catcatcaaa aaggtaaaaa agaagggtca tcaagccgcc catgggggtt cctggaaagt
      601 ggcctatgcc gacttcgtca cggctttgat ggcccttttt ttattgctct ggctcctggc
      661 catgatatct cccgagaaga ggatactgct gtcgcaatac ttcaagcact tcagcgtctt
      721 caaggaatcg gggcagtccg tgattcaggg acactcgtcg ttgttggacc gggacggtgg
      781 cgtggcagcg aagaggatgt tgggttccag ggaatctgat atctcccccg aggggttgca
      841 ggaaaggatc aagggggcca ttgcggaaaa gttgagctcc ctaaaagatc aggtaatagt
      901 cgaggtctta gaggacgacg tgaagataca gatcgtagac acagagggca gcctgatgtt
      961 caagcttggt agcgcccagc cgacggagaa ggcaagacag ctcctgcaac tcctcgccga
     1021 aaacatcagg gatacggcca acaggatcgt gatcgaaggc cacaccgact ctctgcccta
     1081 ccggggtgcc cagattacaa actgggaact ctctacggca agggcctcgg ccgccaggag
     1141 ggagctcgag gccaacggaa tcgagccgtc gcggattgcc agggtcgtgg gctatgcgga
     1201 ccaagagttg ctgatcaagg acgagccgac aaacccaaga aacaggagaa tcagcatcat
     1261 cctgctgaaa gaaaaggcgt ccccacccgc cgatgcggtt gggccggatg cccaaaatcc
     1321 tgcggaaatg accgggctgg caatcaaata aggctcaaag ggtcaattca accttagctt
     1381 cattgcaaag attcatgccc cgccttttcg ttgatcgtca gaaagcagtg tacgacctct
     1441 ctgtcgaact ggaggccggc attcttccgg agttcttcga cggccgtctc gacctccatg
     1501 gccttccggt acggccggtt gttggtcatg gcgtcgaagg agtccgcgac ggcgatgata
     1561 cgagatagca atgggatgtc ctcgccggcg agtccgtccg ggtatccacg tccgtcccac
     1621 cgctcatggt gatggcggat cacgagcctt tccctgtcga agagcatgat gggtttgatg
     1681 atgttttcgc cggtcagggg gtgctgtttg atgatgtcgt attccgcctc ggtcaaccgg
     1741 cccggcttca ataggatgtc gtcgggaata gctattttgc cgatgtcatg gagtatcccg
     1801 acgatcttca tggactcgat gtcctcgggc cggcttccca tcgcctcgca aatcctgacc
     1861 gcaagctcag tgaccctgac ggaatggctc tccgtgtagt gatcccgggc ctggatagaa
     1921 gcggccatgc tcttgagtgt gtccatcagg ttcgcatagg tgctctcgta caggatgttg
     1981 ttctctatgt tcagggatgc ccttttcgtg aggctgagga tgtagttcaa gaccgtgggg
     2041 gtaaactcaa ccccgttttt tctgcgggag atgcccagga caccgaacac cctgtttctg
     2101 atccgcagcg gggccagtat cagagaactc ggtccttcat gaccggaaaa ggagttgatt
     2161 atgcatgcct ctctctcttg gataactttc cggagtatcg aaaggagcga gggtgactgg
     2221 aggtcgagag tgtgttccga atacaggttt ttcactactt tcgggcggaa ctcgcttttt
     2281 tcttcgtcaa aaagcagcag agaacagctt tcaccttgcg agaccttcaa ggccgtgtcg
     2341 gccatctcct cgaaaat
//
LOCUS       DAOTMX010000274         2350 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_35432_length_2350_cov_3.608279, whole genome shotgun sequence.
ACCESSION   DAOTMX010000274 DAOTMX010000000
VERSION     DAOTMX010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35432_length_2350_cov_3.608279"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..2245)
                     /locus_tag="P5573_07550"
     CDS             complement(104..2245)
                     /locus_tag="P5573_07550"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HRT27953.1"
                     /translation="MNNKMSEVFRTDFAGREIFLRTHYLAGQADGAVMVRYGDTVVLV
                     TAVSLKSKREGVDFLPLTVDYEEKTFAAGKIPGGFFKREGRPNEREILISRIIDRSVR
                     PLFPKGYYHETQIVAILLSVDNENDSDVAAMLGCSAALTISDIPFKGPVAGVRVGRVK
                     GQFVANPSAKMLEESDLDLFLTGRKVPRADGNFDVNPVMLEGGAQEVGEDVIIEAVEF
                     GLDALRPVLELQDRMCVAVGRQKRSVEEVSYDGELARKLADLAEKDLAAAYRIPAKLE
                     RYAKLDAARAAALSAVTAEDPAGEQAVRGALDQLERKILREMILNEGTRIDGRSSTDI
                     RPISCEVGLLPRTHGSALFTRGETQALAVLTLGTASDEQRMDYISGEELRSFILHYNF
                     PPFSVGEARMLRGPGRREIGHGALARRALLPVLPSQEVFPYTIRIVSEILSSNGSSSM
                     ATVCGGSLSLMDAGVPVKDAVAGIAMGLLKEGERVVILSDILGDEDHAGDMDFKVCGT
                     KNGVTAMQMDIKIDGVTSEILRKALAQARDGRLFIIDKLGEALPEPRKEISAYAPRIT
                     IMRVKPDKIRDVIGAGGKNIRQIISETGVDINVEDDGTVTIASNDAEAAARAAQLVRW
                     LTEEAEVGKIYLGTVKRVVDFGAFVEILPGTEGLVHISQLAKERVQKVTDILREGDKV
                     AVKVIEIDKVGKIRLSRKEALGEHVPQDRNG"
BASE COUNT          438 a          775 c          578 g          559 t
ORIGIN      
        1 cccgttggtc ttatgctctt cgttgcggga accacagttg acccatacac caagcgagag
       61 ggatctgaca cggtcgattt cctccgagat taccgtgatt ccgttatcca ttacggtctt
      121 gcggtacgtg ttcacccagt gcctctttcc tgctcaaccg gatcttcccc accttgtcga
      181 tttcgatgac ctttacggca actttgtccc cttccctcag tatatcggtg accttctgga
      241 ctctttcctt ggcaagttgt gagatgtgaa ccaacccctc cgtaccgggc aggatttcaa
      301 cgaaggcacc gaaatcgacg actcgtttga ccgtccccag gtagatcttt cccacttccg
      361 cctcttccgt cagccatcgg accagttgcg ccgcccttgc cgcggcctcg gcgtcgttcg
      421 aagcgatcgt gacggtcccg tcgtcttcca cgtttatgtc cacgcccgtt tcactgatga
      481 tctgccggat gttctttccg ccggcaccga tgacgtctct gatcttgtcc ggtttgaccc
      541 tcatgatcgt aatcctcggg gcgtatgccg agatttcctt tctcggctcc ggcaaggcct
      601 ccccgagctt gtcgatgatg aagagtctcc cgtcgcgggc ctgggccagg gccttcctga
      661 ggatttccga ggtcacgccg tcgatcttta tgtccatctg catggcggtc acgccgttct
      721 tcgtcccgca taccttgaag tccatgtcgc ccgcatggtc ctcgtcgccg aggatatcgg
      781 acaagatgac gacccgttca ccttctttca acaaacccat ggcgattccc gccaccgcat
      841 ccttcaccgg gacgcctgca tccatcaggg aaagggatcc tccgcagact gtcgccatgg
      901 aagacgaccc gttcgacgaa aggatctcag agacgattcg tatcgtgtaa ggaaagactt
      961 cttgcgaagg aagtacgggc agcaacgccc gtctcgccag ggcgccgtga ccgatttccc
     1021 ttcgtcccgg tcccctgagc attcgtgcct cgcctacgga aaaaggcggg aaattatagt
     1081 ggaggataaa agaccgtagc tcctcgccac tgatgtaatc catccgctgt tcatcggatg
     1141 ccgttccgag cgtgaggacc gccagtgcct gcgtctcccc cctcgtgaac agcgccgaac
     1201 cgtgggtcct cggaagaagc ccaacctcgc aactaatcgg gcgaatgtcc gttgaggacc
     1261 taccgtctat ccgggtcccc tcgttgagga tcatttcccg caaaatcttc ctttccagct
     1321 gatccagggc gccacgcacc gcctgctcgc cggcagggtc ttccgcagtg accgcactca
     1381 gcgcggcggc cctcgccgcg tcgagcttcg cgtatctctc cagttttgcc ggtatccggt
     1441 aggctgcggc aaggtccttc tccgccaggt cggccagttt tctcgctaat tccccgtcgt
     1501 aggaaacttc ttcgaccgac ctcttttgcc tccccaccgc cacgcacatg cggtcctgga
     1561 gttccaggac gggccgtagc gcatcgagac cgaactcgac tgcctcgatg atgacgtctt
     1621 cccctacttc ctgggcaccg ccttccagca tcacggggtt gacgtcgaag ttgccgtcgg
     1681 cacggggtac cttcctgccg gtcaggaaga ggtcaaggtc gctctcctcc aacatcttgg
     1741 ccgaagggtt cgccacaaac tgtcctttta ccctgcccac ccgcacgccg gccaccggcc
     1801 ccttaaaggg aatatccgag atcgtcaagg cggcggaaca ccccagcatg gccgccacgt
     1861 ccgaatcgtt ctcattgtcg acggacagaa gaatagccac gatctgcgtc tcgtggtaat
     1921 agcctttcgg aaacagaggc cgcacggagc ggtcgatgat cctcgatatc agaatctcgc
     1981 gctcattcgg gcgtccctcc ctcttgaaaa agccgcccgg gattttaccc gcggcgaatg
     2041 tcttctcttc gtaatcgacc gtcagcggca ggaaatccac cccttctctc ttgcttttca
     2101 gagaaaccgc cgttaccaga acaaccgtat cgccgtaccg gaccatgacg gcgccgtccg
     2161 cctgaccagc taaataatgg gtcctcagga atatttctct tccggcaaaa tcagttctaa
     2221 aaacttcact cattttatta ttcattacct cctatggctt taacctgccg cttattaaaa
     2281 tccttctttt gcttcatctc cgctaccttc gtatgccgag gcgctggatg agcgtcctgt
     2341 atctctcgat
//
LOCUS       DAOTMX010000275         2345 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_35528_length_2345_cov_3.928821, whole genome shotgun sequence.
ACCESSION   DAOTMX010000275 DAOTMX010000000
VERSION     DAOTMX010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35528_length_2345_cov_3.928821"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="P5573_07555"
     CDS             <1..754
                     /locus_tag="P5573_07555"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HRT27954.1"
                     /translation="GIAIGTASLPSLSAQVARGEIEAMKKTLTFSLRLILFIAIPAMI
                     ALVALREPIISVLFQRGEFDAQSTALTAQALFYYSLGLWAFSGIRVVVSAFYSFQDTK
                     TPVYVGIVALVVNTVFSIILMFPLKHGGLALATSIATAVNMITLSVILKRRIGRFLDR
                     EFYRSVSLSTVASLIMWAGIASIDLFLPWNEGAPFMEKLSVLSLEIAAGLAVFLVSSY
                     MLKSSELAAALEILRHRLTRRPTKIKTEYKNH"
     gene            855..2135
                     /gene="serS"
                     /locus_tag="P5573_07560"
     CDS             855..2135
                     /gene="serS"
                     /locus_tag="P5573_07560"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003218092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HRT27955.1"
                     /translation="MLDIKFLRENLDFVRRKMLERGQEIDLDRFAALEAKRRNILQEV
                     ESLRSERNQASKEIGRKKAGKEDASAIIARMAEVSARIKELDELLKETEEDLRNFVMT
                     IPNIPHESVVYGTSSEDNPVIRAWGEKPAFDFIPKPHWEIGEDLNILDFGRGAKITGA
                     RFTLYRGLGAQLERAVVNFMLDLHTGNHGYLEVLTPFMVNKESMTGTGQLPKFAEDLF
                     KIEGLDYYLIPTAEVPVTNIHREEILEEKMLPVYYVAYSPCFRAEAGSYGKDTRGLIR
                     QHQFNKVELVKFTAPEASYDELEKLTLNAEEVLKRLGIHYRTVSLCTADLGFSSAKTY
                     DIEAWLPGQETYREISSCSNFEDFQARRASIRFRREESGKVEFVHTLNGSGLAAGRTV
                     VAILENYQQKDGSVVIPDVLRPYMKGIDRIPPTG"
BASE COUNT          525 a          706 c          650 g          464 t
ORIGIN      
        1 cgggatcgcc atcggtaccg cctccctccc gagcctgtcg gcacaggtcg ccagggggga
       61 aatcgaggcg atgaaaaaga ccctcacctt ttccctgagg ctcatcctct tcatcgccat
      121 cccggcgatg atcgccctgg tggcgttgcg cgaaccgatc atttccgtcc tttttcaacg
      181 gggggagttc gacgcacaat cgacggcgct caccgcccag gccctcttct actattccct
      241 cggcctctgg gccttttccg gcatccgtgt cgtcgtctcg gctttctatt ccttccagga
      301 tacgaagacg ccggtctacg tcggtatcgt ggccctcgtc gtcaacaccg tcttcagcat
      361 catcctcatg tttcccctca agcatggggg gctggccctc gccacctcca tcgccacggc
      421 agtcaacatg attaccctgt ctgtcatcct gaaaaggagg atcgggcgat tcctggaccg
      481 ggagttctac cgttccgtgt ccctgtccac cgtcgcttcc cttatcatgt gggcgggcat
      541 cgcctcgatc gacctcttct tgccctggaa tgagggcgca cccttcatgg agaagctctc
      601 cgtcttatcg cttgagatcg ccgccggtct cgccgtgttc ctcgtttcct cttacatgct
      661 gaaatcgagt gagcttgcgg cggcgctgga aatcctcagg cacaggctca cccgccgccc
      721 cacgaagata aaaacagaat acaaaaacca ttgacttcaa taccccaaaa tgataggaga
      781 atccctcctg tttgagagga aaaacgtgcc tataccgcaa catatccccc atcgggaacg
      841 aaaggctgcc gataatgctt gacatcaaat tcctccggga aaacctcgac ttcgtccggc
      901 gtaagatgtt ggagcggggc caggagatag acctcgaccg gttcgccgcc ctggaagcaa
      961 agcgccgcaa catcctccag gaagtggagt ccctgcggag cgagcgcaat caggcctcga
     1021 aggaaatcgg ccggaaaaag gccggcaagg aggacgcctc ggcgatcatc gccaggatgg
     1081 cggaggtttc ggcgcggatc aaagagctcg acgagctact gaaagagacc gaagaagatc
     1141 tgcgcaattt cgtgatgacc atacccaata tcccccacga gtcggtcgtt tacggtacga
     1201 gttccgagga caacccggtg atccgggcgt ggggcgaaaa accggccttc gatttcatcc
     1261 ctaaaccgca ctgggagatc ggcgaagacc taaacatcct cgatttcggc aggggcgcga
     1321 aaatcacggg tgcgaggttc acgctctacc gggggctggg ggcgcaactg gagcgggcgg
     1381 tcgtcaactt catgctcgac ctccacacgg ggaaccacgg ttacctcgag gtcctcacac
     1441 cgttcatggt gaacaaggag agcatgacag ggacgggaca gcttcccaaa ttcgccgaag
     1501 acctcttcaa gatcgagggc ctggactact acctgatccc tacggcggag gtgccggtga
     1561 ctaacatcca ccgggaggag atactggaag aaaagatgct tcccgtctat tacgtcgcct
     1621 actcgccctg tttccgcgcc gaggccggct cttacgggaa agatacccgc ggcctcatcc
     1681 gccagcacca gttcaataag gtcgagctcg tcaagttcac ggcaccggag gcctcatacg
     1741 acgaactgga aaaactgacc ctcaacgccg aggaggtgct gaagcgcctt ggcatccatt
     1801 accgcaccgt gagcctctgc acggcggacc tggggttctc atcggccaag acctatgaca
     1861 tcgaggcgtg gttgccgggc caggagacat accgggagat ctcttcctgc agcaacttcg
     1921 aggacttcca ggcgagacgg gcctcgatcc gcttccgaag ggaggaaagc ggtaaggtgg
     1981 agttcgtcca cacactgaac ggctccggcc ttgcggcggg aaggaccgtc gttgcgatcc
     2041 tggaaaatta ccagcagaag gacggcagcg tcgtcatccc cgacgtcctc aggccataca
     2101 tgaaaggtat tgacagaata ccccccacgg gataacattt ttaaacgcga atcggcttat
     2161 gttcttccca tgaacgggca ggagcaagaa atcttctctt tgccgttaga cacagaccct
     2221 ttccccagct gaaagtacgc tttcggatga accgggatgt aatgttaggg tcgaaaaatc
     2281 atatataggt cggagggatg gccgagtggt ctatggcggc ggtcttgaaa accgttgacc
     2341 gaaag
//
LOCUS       DAOTMX010000276         2339 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_35654_length_2339_cov_3.381786, whole genome shotgun sequence.
ACCESSION   DAOTMX010000276 DAOTMX010000000
VERSION     DAOTMX010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35654_length_2339_cov_3.381786"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="P5573_07565"
     CDS             <1..266
                     /locus_tag="P5573_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27956.1"
                     /translation="EREIREETGLIIRAGKPFYTFDIIQRGENGRIEFHYVIVDMAAD
                     YVGGAPQAGDDAVDARWVSAGELGSLQVTPTTLDLLKEIRFCR"
     gene            complement(317..745)
                     /locus_tag="P5573_07570"
     CDS             complement(317..745)
                     /locus_tag="P5573_07570"
                     /inference="COORDINATES: protein motif:HMM:NF025892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HRT27957.1"
                     /translation="MERTFEAIHKKVEEEPYAKKLGLRLVKVAPGYSLVRMVFTPDME
                     NIFGMAHGGAIFSLIDEAFETASNSHGTVAVALSLNVNYIKSPSPGAVLQAEAKETSR
                     SNRIATYDIEVRDDGGDLVARSQATVYRKKDRLPFLTETE"
     gene            913..2181
                     /locus_tag="P5573_07575"
     CDS             913..2181
                     /locus_tag="P5573_07575"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRT27958.1"
                     /translation="MRVLLISANTEKINILPLPLGLHYVAAATRNRGHDVRVLDLMAY
                     EESSQPIRDAIESFRPGLIGISVRNIDDQDMHDPKFLLADVKNAVLQCRRLSAAPVVL
                     GGAGYSIFPEAVLAYLGADMGIQGEGEYVFPELLERLEKGMGLSGLPGLYLRGIGLQG
                     ERTFIDDLSDLDITVVRSFLSAYDRGLFVPFQTRRGCPMDCTYCSTAAIEGRRIRKKA
                     PQSAVEELRAFVGQGFRRFFFVDNTFNLPSSYAKEICRAIIRQGLGIDWWCILYPGRV
                     DEDLIRLMAEAGCKEVSLGFESGCERILKVMNKRFGPEEIRKTSLMLEKNGIQQTGFL
                     LLGGPGETRESVLESLSFADSLPLNGLKITQGIRIYPHTKLARIAVSEGMVAPDDDLL
                     KPTFYMVRGIEDWLRETLGAWMAERGHWTA"
     gene            2148..>2339
                     /locus_tag="P5573_07580"
     CDS             2148..>2339
                     /locus_tag="P5573_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27959.1"
                     /translation="MDGRAGPLDSLGRRNPQSVERASLSCQADSAKRKLTMNSPDGFR
                     ENEALIILCVEVTLVMLGIG"
BASE COUNT          479 a          632 c          721 g          507 t
ORIGIN      
        1 ccgaacggga gataagagaa gagacgggcc tcatcatccg ggcgggaaag ccgttctata
       61 ccttcgacat catccagcgc ggtgagaacg gccgcatcga attccattat gtcatcgtgg
      121 atatggcggc ggattacgtc ggcggcgccc cgcaggccgg ggacgatgcg gtggacgcgc
      181 gctgggtttc ggcgggggaa ctggggtctc ttcaggtgac gcccacgacc cttgacctcc
      241 tcaaggagat acgcttttgc cggtgaccgg ccggcgggga aagagccgtt tttgtacttt
      301 accggagctc gggcgtctat tctgtttccg tcaggaaagg gagccggtcc tttttccggt
      361 acacggtggc ctgggaacgg gcgacgagat caccgccgtc gtcccggacc tctatgtcgt
      421 aggtcgctat ccggttggaa cggctggttt ccttcgcctc ggcttggagc accgcgcccg
      481 gggaagggga cttgatgtaa ttgacgttga gactcagtgc gacggcaacg gtgccgtggg
      541 aattagaagc cgtctcgaag gcttcgtcga tgagggagaa gatggccccg ccatgggcca
      601 tcccgaagat gttttccatg tcgggggtaa agaccatcct tacgagggaa tagccgggtg
      661 cgactttcac cagtctcagg cccagtttct tcgcgtaagg ttcttcctct accttcttgt
      721 ggatggcctc gaatgtcctt tccactccct ccctcctctt ctgcagtgac gctttggccg
      781 ccggatgtaa ataccgtctg acgggattat ctgtcaaggc ccttgcccgt tttccgaccg
      841 acctgacccg cgtatgccgt cggataagcg gtatcagcct attttggaga atgttttccc
      901 gatgaaggga atatgcgagt actgttgatt tccgccaata cggaaaagat aaacatcctg
      961 ccgctgcccc tgggcctgca ttacgtggcc gcggctacgc gaaacagggg gcacgacgtc
     1021 agggtgctcg accttatggc ctatgaagaa tcatcgcaac cgataaggga cgcgatagag
     1081 tcgttccgtc ccggtttgat cggcatctcc gtgcggaata tagacgacca ggatatgcac
     1141 gacccgaagt tcctgctcgc tgatgtaaaa aatgccgtcc ttcaatgccg tcgtctttcc
     1201 gctgcccccg tcgtcctggg cggcgccggt tacagcatct ttcccgaggc cgttctcgcc
     1261 tacctgggtg cggacatggg cattcagggg gagggggaat acgtcttccc ggaactgctg
     1321 gagaggttgg agaagggcat ggggctttca ggactgcccg gattgtacct gagagggatc
     1381 ggtctccagg gagaacgcac cttcatcgat gacttgagcg acctggatat caccgttgtc
     1441 cggagttttt tgtccgccta tgaccgggga ttgttcgttc ccttccaaac gaggcgcggc
     1501 tgcccgatgg attgcactta ttgctcgacc gctgcgatcg aaggccgccg aatcaggaaa
     1561 aaggcccccc agagtgcggt cgaagagctt agggcctttg tagggcaagg tttccgccgg
     1621 ttctttttcg tcgacaacac cttcaacctg ccttcctcct acgccaaaga gatctgccgt
     1681 gcgatcatcc ggcagggctt gggtatcgac tggtggtgca tcctctatcc cgggagggtc
     1741 gatgaagacc tgataaggct gatggcggag gcaggctgca aagaggtcag cctgggtttc
     1801 gagagcggtt gtgagaggat actcaaggtc atgaacaaga ggtttggacc ggaagagatc
     1861 agaaagacat ccctgatgct cgaaaaaaac gggattcagc agacgggttt tttgctgctc
     1921 ggcggaccgg gagaaacaag ggaatcggtg ctggagagtc tgagcttcgc cgattcgtta
     1981 ccgttgaacg gcctgaagat aacgcaggga atccgcatct acccccatac gaaactggcg
     2041 cgaatagccg taagcgaggg gatggtcgcg cctgacgacg acctcctaaa gcccaccttc
     2101 tacatggttc gcgggatcga ggattggctc agagaaacct taggtgcttg gatggccgag
     2161 cggggccact ggacagctta gggaggcgta atcctcagtc cgtcgaaagg gcatccctct
     2221 catgccaagc cgacagcgca aaacgtaaac tcacgatgaa cagccccgac ggtttccgag
     2281 agaacgaggc cctcatcatt ctctgtgtcg aggtcacact ggtgatgctg gggataggg
//
LOCUS       DAOTMX010000277         2314 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_36145_length_2314_cov_3.291279, whole genome shotgun sequence.
ACCESSION   DAOTMX010000277 DAOTMX010000000
VERSION     DAOTMX010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36145_length_2314_cov_3.291279"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..984
                     /locus_tag="P5573_07585"
     CDS             <1..984
                     /locus_tag="P5573_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRT27960.1"
                     /translation="APTSESWAGRAEIVAGDEKMLSLLEMLKCVAISKASIFIQGESG
                     TGKELIARHIHRYSNRAGMPFVAVNCAAVPYNLLESEMFGYEKGAFTGAVQRKIGKFE
                     MADGGTLLLDEVSEMDIHLQAKLLRAIQESEIDRLGGKEPVRVNVRIIATTNLDPKKC
                     VAEKKFREDLYYRLNVIPIKVPPLRERKGDIPCLVDHFLKKFSALQGREKPEVSAEAF
                     ELLQAYLWPGNVRELENVMERAVLICSGSQIRPEHLRLDGEEVGERPPEGEPDEPSAP
                     VYRNVTLYEMERQLIFDTLKQVKGNKTKASKILGISVRTMRNKLNEYGAPS"
     gene            1148..1531
                     /gene="flgB"
                     /locus_tag="P5573_07590"
     CDS             1148..1531
                     /gene="flgB"
                     /locus_tag="P5573_07590"
                     /inference="COORDINATES: protein motif:HMM:TIGR01396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HRT27961.1"
                     /translation="MEQLFGKTIDILTAVIGYRSERHKVILSNLVNIDTPDYGPKDLV
                     FKTELDNAARGRIGVTLTTTNGRHIGSPSFPGKGTEVVDREDEEVSLDREMMDLAENH
                     LMYNTTVELLARKFRGLKSAIQEVR"
     gene            1533..1958
                     /gene="flgC"
                     /locus_tag="P5573_07595"
     CDS             1533..1958
                     /gene="flgC"
                     /locus_tag="P5573_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HRT27962.1"
                     /translation="MDFFVPFRICGSGLTAQRAKMDVITANLANVDTTRTPEGGPYMR
                     KVVSFSSKPVGRVFDMKLRKALNMVSVGDVEESKEGVRRVADPGHPDADASGFVSLPN
                     VNVVSEMAGMIMASRAYEACVTAFDATKSMALKTLEIGR"
     gene            1977..2279
                     /gene="fliE"
                     /locus_tag="P5573_07600"
     CDS             1977..2279
                     /gene="fliE"
                     /locus_tag="P5573_07600"
                     /inference="COORDINATES: protein motif:HMM:NF014143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HRT27963.1"
                     /translation="MNDIIIGGNPALTPVEPGKAEKEGSGTQTPFGTVLKKTIKDIND
                     LQIKADQAIAKVEVDNSASIHEAMIALEKADISFKAMMQVRNKIIEAYQEIMKMQV"
BASE COUNT          583 a          584 c          736 g          411 t
ORIGIN      
        1 gcgccgacca gcgagagctg ggcggggcgc gcggagatcg tggcggggga cgagaagatg
       61 ctatctctcc tcgagatgct gaagtgtgtc gcgataagca aggcgagcat cttcatccag
      121 ggagaaagcg ggacgggcaa ggagttgatc gcccgtcata tccatcgcta cagcaaccgg
      181 gccggcatgc ccttcgtggc cgtgaattgt gccgccgtcc cctacaacct cctcgagagt
      241 gagatgttcg gctacgaaaa aggggcattc accggcgcgg tgcagagaaa gatcggaaag
      301 ttcgagatgg ccgacggcgg cacgctgctc ctcgatgaag taagcgagat ggacatccat
      361 ctccaggcca aactgttgag ggccatccag gaatcggaaa tcgaccgact cgggggtaaa
      421 gagcccgtcc gggtgaacgt gcggataatc gccacgacaa acctggaccc gaagaagtgc
      481 gtcgccgaaa aaaaattcag ggaagacctg tactatcgac tgaacgtcat cccgataaag
      541 gtgccccccc tcagggagag aaaaggcgac atcccttgtc tcgtcgacca tttcctcaaa
      601 aagttcagcg cccttcaggg aagggaaaag ccggaggttt cagcggaggc cttcgaattg
      661 ctgcaggcct atctctggcc ggggaacgtg cgggagctgg aaaacgtgat ggagcgggcc
      721 gtcctcatat gcagcggcag tcagatcagg ccggagcatc tgcgcctcga cggggaagag
      781 gtcggggaaa ggccgcccga aggggaaccc gatgaaccgt ccgctcccgt ttacagaaac
      841 gttaccctgt acgagatgga gaggcaactc atcttcgaca cgctgaaaca ggtaaaggga
      901 aacaagacca aggcgtcgaa gatcctggga ataagcgttc gcaccatgag aaacaagctg
      961 aacgagtacg gcgcgccgtc ttaggcaggg ctccggggtg ggaaaaaatt tccgcccccc
     1021 tgtagcgggg ctgccgggcg agaatccgct gcagtgaccg gcttgcggag cgggacccgg
     1081 tttcgggagg caaaaggtaa ctggtatgcg ctttgcttat tgattgagtt gattctcagg
     1141 aggccttatg gagcaattat tcgggaaaac tattgacatc cttacggcgg tcatcggtta
     1201 ccgttcggag aggcacaagg tcatactgtc caatctggtg aacatcgaca cgccggatta
     1261 cgggcccaag gaccttgttt tcaaaaccga acttgataat gccgccaggg gcaggattgg
     1321 cgttacacta acgacgacga atgggcgcca tatcggcagt ccgtcatttc ccggaaaggg
     1381 gacggaagta gttgacaggg aagacgaaga agtttccctg gacagggaga tgatggacct
     1441 tgcggagaac catctcatgt acaacacgac ggtcgagctc ctggcgagga aattcagggg
     1501 cttgaaaagc gccatacagg aggtcaggta gtatggattt ttttgtacct tttaggattt
     1561 gcggctccgg tcttaccgca cagcgggcga agatggacgt aatcacggca aacttggcga
     1621 acgtcgacac gacgcggacg ccggaagggg gtccttacat gcgcaaggtc gtttctttct
     1681 cttccaagcc ggtcggccgt gtcttcgaca tgaaactacg gaaggccttg aacatggtca
     1741 gtgtcgggga cgtcgaagaa agtaaagaag gcgtaaggag agtggccgac ccgggccacc
     1801 cggacgcgga tgcctcggga ttcgtgtcgc tgcccaacgt gaacgtggtg agcgagatgg
     1861 ccgggatgat aatggcgagt agggcttacg aggcctgcgt cactgccttc gatgcgacga
     1921 agagcatggc gctcaagacg ctcgaaatcg gacgctgacg gagatggagg ccggtcatga
     1981 atgacatcat catcggggga aaccccgccc tgacgccggt tgaaccgggg aaggcggaaa
     2041 aggaagggag cggcacccag acgcccttcg gtacggtact taagaaaaca atcaaggata
     2101 tcaacgacct tcagataaaa gcagatcagg ccatcgcgaa agtggaggtc gataactcgg
     2161 cgagcatcca tgaggcgatg atcgccctcg aaaaggccga catctccttc aaggccatga
     2221 tgcaggtaag gaacaagatc atcgaggctt accaggaaat catgaagatg caggtttagc
     2281 gaggtctttc ggtccgtgag gacggctccg ccga
//
LOCUS       DAOTMX010000278         2314 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_36146_length_2314_cov_3.154493, whole genome shotgun sequence.
ACCESSION   DAOTMX010000278 DAOTMX010000000
VERSION     DAOTMX010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36146_length_2314_cov_3.154493"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..992)
                     /locus_tag="P5573_07605"
     CDS             complement(<1..992)
                     /locus_tag="P5573_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRT27964.1"
                     /translation="MDYLVTGGAGFIGSNVAEALLKQGCRVRVLDNFLTGKRDNLTFA
                     KPGDVDLIEGDIRDLATCRRAVEKVDYVIHLAALGSVPRSVADPLMANEINVTGTLNV
                     LVASRDAGVKRLVYASSSSVYGDQPTDGQPDSADGPTPKVETMTPNPLSPYAVNKLAG
                     EAYCRVFYRLYGMETVALRYFNVFGMRQDPASEYAAVIPRFISALLEGRQPVVYGDGK
                     QSRDFTFVEDVVSATLLACTAPVEAAGRVFNIACNRRFTLLELLAELKEILGSATEPV
                     FAEARKGDIRHSMADIALAKKYLRFEPRVSFRRGLERTVDWFRSRPYNPIPSSSP"
     gene            complement(1011..1997)
                     /locus_tag="P5573_07610"
     CDS             complement(1011..1997)
                     /locus_tag="P5573_07610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HRT27965.1"
                     /translation="MRKNLIVGLVVGALLVYFSFKDIDMGSVAEGFKSVRCGYAFLAL
                     FCLFLMQVLRSYRWGLLLAPLDKIGQFDLFAVTSVGFLAVVAVPARLGELARPYLIAG
                     KSNVSMAVALGTVFVERIFDILTILAVFFLLLLFIPLPPWLAKAGMVSLLAALAALAL
                     LLFLIFKREEAGRLLTPVFKRLPESLNRKVEALLRHFIDGLAVLSKVKTTFSVAGLSV
                     LIWVVDAAAVYLLFLSFGFDLPFAAAFVVMVVLIIGIAIPTAPGFIGNWHFFCILGLS
                     LFGVPKADALSYALIFHALGVGVIIFLGLAFLPFNRFSLADIRRRADINVNL"
     gene            complement(2067..>2314)
                     /locus_tag="P5573_07615"
     CDS             complement(2067..>2314)
                     /locus_tag="P5573_07615"
                     /inference="COORDINATES: protein motif:HMM:NF023514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3568 family protein"
                     /protein_id="HRT27966.1"
                     /translation="FDTVWAAVEKTVADMKAVDVLPEKEIAQGKITASINDEKVTFSL
                     KYKSKDQTTVSVRVGVLGNKLSSQLLHDKIADNLRKK"
BASE COUNT          520 a          656 c          717 g          421 t
ORIGIN      
        1 ggagacgaag aagggatagg gttgtacggt cgagaccgga accaatccac ggtccgctcc
       61 agccccctcc ggaaagatac ccttggctcg aaccgcaggt atttctttgc gagggcgatg
      121 tccgccatgg agtggcggat gtcgcccttt cttgcttcag caaagaccgg ttccgtggcc
      181 gaacccagga tttctttcag ttcggcaagg agttcgagga gagtgaagcg cctgttgcag
      241 gcgatattga acaccctccc cgcggcttcc acgggcgcag tgcaagccag cagtgtcgcg
      301 gaaacgacat cctcgacgaa cgtgaagtct cgcgattgtt tcccgtctcc gtagacgacg
      361 ggttgccggc cttcgaggag cgccgagatg aaacggggga tgacggcggc gtactccgac
      421 gccgggtcct ggcgcatccc gaagacgttg aagtaccgca gggcgacggt ttccatgccg
      481 tagagacggt agaatacccg gcagtaggcc tctccggcga gcttgttcac ggcgtaaggg
      541 gaaagggggt tgggcgtcat cgtttcgacc ttgggcgttg ggccgtccgc tgagtccggt
      601 tgtccgtcgg tgggctggtc gccgtagacg gaggaggagg aggcatacac gagacgcttg
      661 acgccggcgt cgcgggaagc gacgagcaca ttgagcgtcc cggtgacgtt gatttcgttt
      721 gccataaggg ggtcggcgac ggaacggggc acggacccga gggcggcaag atggatgacg
      781 tagtccacct tttccactgc ccgccggcag gtggcgaggt cgcggatgtc cccttcgatc
      841 agatcgacat ctcccggctt agcgaaggtc agattatccc gcttcccggt gaggaagttg
      901 tccagcaccc ttacccggca gccttgtttg agcaaggcct cagccacgtt cgacccgatg
      961 aaccccgccc cgcccgttac cagataatcc atgttacccc ctcttttgat tcaaagattt
     1021 acgttgatgt cggcccttct gcggatgtcc gccagggaaa acctgttgaa gggcagaaat
     1081 gccagtccca ggaatatgat gactcccacg cccagtgcat gaaaaataag ggcgtaactc
     1141 agggcgtccg ccttgggaac gccaaagagg gacaacccga gaatgcagaa gaaatgccag
     1201 ttaccgatga atcccggtgc cgtcgggatc gcgatgccga tgatcagaac taccatcacg
     1261 acgaaggccg ccgcgaaggg caggtcgaaa ccgaaagaaa ggaatagcag gtagacggcg
     1321 gccgcgtcga ctacccagat cagcaccgag aggccggcca cggagaaagt cgttttcacc
     1381 ttagacagca cggcaagccc gtcgatgaaa tggcgcagga gggcctcgac cttccgattc
     1441 aggctttcgg gaagccgctt aaaaaccggg gtcaacagac ggcccgcttc ctctctcttg
     1501 aaaatcaaga agagcagaag tgccagcgcc gccagtgccg caagcaggga caccataccg
     1561 gccttcgcca gccaaggggg gaggggtatg aacaagagca acaggaaaaa gacggcgaga
     1621 atggtgagga tgtcgaagat gcgctcgacg aacaccgtcc ccagggcgac ggccatgctg
     1681 acgttgctct ttccggcgat caggtaaggc cttgccaatt cgccgagcct tgccgggacg
     1741 gctaccacgg caagaaagcc cacactggtc accgcaaaga ggtcgaactg tccgatttta
     1801 tcgagcggcg ccagcagcag gccccagcgg tatgaccgga ggacctgcat caggaagaga
     1861 cagaaaaggg cgaggaaggc gtaaccgcac cggacggact taaacccctc ggcgacggaa
     1921 cccatgtcga tgtccttgaa agaaaaatag accaggaggg cgccgaccac gagacccaca
     1981 atcaggttct ttctcatcct tttcccgcaa aaaaatgccc ggtcggcata aactgggaaa
     2041 aaggcagtcc ggccggccgg gttttcttat ttcttcctca ggttgtcggc gatcttgtca
     2101 tggaggagtt gcgaagagag tttgttcccg agcacgccca cgcggacgga gaccgtcgtc
     2161 tgatccttcg acttgtactt cagggaaaag gttacctttt catcgttgat cgaagcggtg
     2221 atcttgccct gggcgatttc cttctccggc aggacgtcca cggccttcat gtcggcgacg
     2281 gtcttttcta cggcggccca tacggtgtcg aagg
//
LOCUS       DAOTMX010000279         2304 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_36361_length_2304_cov_3.210760, whole genome shotgun sequence.
ACCESSION   DAOTMX010000279 DAOTMX010000000
VERSION     DAOTMX010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36361_length_2304_cov_3.210760"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="P5573_07620"
     CDS             complement(<1..565)
                     /locus_tag="P5573_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27967.1"
                     /translation="MKRISIILLAVGLVAALSVPVFALDVKVTGRAEMTGYWESNRAM
                     VDNNEASQRYFTTFIRIEPIFKVAEGLQLVTRFEGLERVAGLDRIGYEQWHKTASAIG
                     GTPWASMMDRNSENEQNFAMKRAYIDANILGGRWWVGYQQGGNFGTDFLDYYEDVLRV
                     KAHYFVGPFTFIALWEKPGENSLGNPGY"
     gene            953..>2304
                     /locus_tag="P5573_07625"
     CDS             953..>2304
                     /locus_tag="P5573_07625"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HRT27968.1"
                     /translation="MAEEKKVYPVPERVAKTAYINSMEMYNKIYKRSIEDPQNFWSEI
                     AGEYVEWFKKWDKAEEYNFDIRKGPIYLKYFEGGKLNVSYNCVDRHLKTRPDKVAIQW
                     EGNEPGEERALTYRQLHEEVCKFANVLKAHGIKKGDRVQIYLPMIPELAIAMLACTRI
                     GAVHSIVFGGFSAESLRDRIEDCRSKLLVTCDGTFRGAKAVPQKDNADKAIEGFDFVE
                     KMIVVRRVGDKIKCKWDPKKDLWWHEEMAKVNADCPPEWMDAEDPLFILYTSGSTGKP
                     KGVLHTTGGYLVFVAYTHKIIFDYHEEDIYWCTADIGWVTGHSYIVYGPLCNGATSVM
                     FEGVPNYPDISRFWKIVEKYKVTIFYTAPTAIRAIAKEGDEWVKKADISTLRILGTVG
                     EPINPEAWNWYYNVIGRGQCPIVDTWWQTETGGILITPLPGATPLKPGMATLPFFGVW
                     P"
BASE COUNT          547 a          660 c          615 g          482 t
ORIGIN      
        1 tgtaccccgg attgcccaag ctgttctcac cgggcttttc ccagagggcg atgaacgtga
       61 agggcccaac aaagtagtgt gccttcactc ggaggacgtc ttcatagtaa tccaggaagt
      121 ccgtaccgaa gttgccgcct tgctggtaac cgacccacca ccggccgccg aggatgttgg
      181 cgtcgatgta ggcacgcttc atggcaaagt tctgctcgtt ctcggagttt ctatccatca
      241 tggacgccca aggagtgccg cctattgctg atgcagtctt atgccattgc tcatagccaa
      301 tcctatccag accggcgacc ctctccaagc cctcgaaccg ggtcaccagc tggagacctt
      361 cggcaacctt gaatataggc tcgatccgga tgaaggtggt gaagtacctc tgggacgcct
      421 cattgttgtc gaccatcgct ctgttgctct cccagtatcc cgtcatttcc gcccgacccg
      481 tgactttcac gtccagggcg aaaacaggca cgctgagagc agccacaagg ccgacggcga
      541 gcaagataat cgatattctc ttcatgtctt cctcctgttt tagttttggt tttggtccgc
      601 ctcccttgca cgtttcggag gtctctgacc atctctactt tcctaaaacg cctccataca
      661 tctcaaccga atgatcgctc tgtccggcac cacctcctcc ctcgaccccc gttacatagg
      721 gataataccc cgaatgtcca ttcgggttcg gcaaaaccta acaccaagga aaaaaggtgt
      781 caagtaattt ttcatctaaa ttttccttga cagggaaggg aacgctaaat tatatttcgc
      841 gggatttgga aggggattat tacgggttaa aggtattctt ggctttcgtc aggttgagac
      901 gggaatgccc tcccctaaaa tatcaaaagt actatggagg aggtcaagaa gtatggcaga
      961 agaaaagaag gtatatccgg taccggagag ggtcgcgaag acggcttaca tcaacagcat
     1021 ggagatgtac aacaagatct acaagaggtc gatcgaagac ccccagaatt tttggtccga
     1081 aatcgcggga gagtacgtag aatggttcaa gaagtgggac aaggccgaag agtacaactt
     1141 cgacatccgc aagggtccca tctacctgaa gtatttcgag ggcggcaagc tgaacgtctc
     1201 ttacaactgt gtggacagac acctgaagac gaggcccgac aaggtcgcca tccagtggga
     1261 gggcaacgaa ccgggcgaag agcgggcgct cacctacagg cagctccacg aggaagtatg
     1321 caaattcgcc aacgtcctga aggcccacgg catcaagaag ggtgaccgtg tccagatcta
     1381 tcttcccatg atccctgaac tggccatcgc gatgctcgcc tgtacgcgca tcggcgcggt
     1441 acacagcatc gtgttcgggg ggttcagcgc cgagtccctg cgcgaccgca tcgaggactg
     1501 caggtccaaa ctcctggtca cctgtgacgg gaccttccgc ggcgccaagg ccgttcccca
     1561 gaaagacaac gccgacaagg ctatcgaggg tttcgacttc gtggaaaaga tgatcgtcgt
     1621 ccggcgagtg ggcgacaaga tcaaatgtaa atgggatccc aagaaagacc tctggtggca
     1681 cgaggaaatg gcgaaggtaa atgccgattg cccccccgag tggatggacg ccgaagaccc
     1741 gctctttatc ctctacacat cgggctctac gggtaaaccc aaaggcgttc tgcacaccac
     1801 gggcggttac ctcgtttttg tcgcttacac ccacaagatc atcttcgatt accatgaaga
     1861 agacatctac tggtgcacgg cggatatcgg ctgggtcacg ggccacagct acatcgtcta
     1921 cggccccctc tgcaacggcg ccacttcggt gatgttcgag ggtgttccca actaccccga
     1981 cataagccgt ttctggaaga tcgtcgagaa gtacaaggtc accatcttct acaccgcgcc
     2041 cacggccatc cgagctatcg ccaaggaagg cgacgagtgg gtcaaaaagg cggacatctc
     2101 cactctgcgt atcctcggaa ccgttggtga acccatcaac cccgaggcct ggaactggta
     2161 ttacaacgtc atcggtcgtg gccagtgtcc gatagtcgat acatggtggc agacggagac
     2221 gggcggaatc ctgatcacgc ccctgcccgg cgcgacgccc ctgaaacccg ggatggcgac
     2281 gctcccgttc ttcggcgtct ggcc
//
LOCUS       DAOTMX010000280         2302 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_36395_length_2302_cov_3.822875, whole genome shotgun sequence.
ACCESSION   DAOTMX010000280 DAOTMX010000000
VERSION     DAOTMX010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36395_length_2302_cov_3.822875"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="P5573_07630"
     CDS             <1..226
                     /locus_tag="P5573_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27969.1"
                     /translation="RNPRLVEHRGGCVEPHRMESVVGEDAGEIPCPAPQVQNPPVAAL
                     FQEADQVPAEIAEEPVQRFPQEGEKGAMGR"
     gene            complement(363..1025)
                     /locus_tag="P5573_07635"
     CDS             complement(363..1025)
                     /locus_tag="P5573_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27970.1"
                     /translation="MRRGNKSFWPALLSIIVVMTAASAAVAGMAVFLKDGRVVQVPVN
                     KEDVVGISFEETAMPASGNITWDFETGDLRGWNATGNAFSNQPTYGDNPTARHRGQPS
                     NHQGNYWIGGYENRHRPSDPPGRVQGDGPQGTLTSDSFTITSPRISFLIGGGCDINTE
                     RVELLVNGQAVLRATGKCTETMGRITWDVSAYRGQSAQIRLIDASSGGWGHINFDDVR
                     FE"
     gene            complement(1175..>2302)
                     /locus_tag="P5573_07640"
     CDS             complement(1175..>2302)
                     /locus_tag="P5573_07640"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRT27971.1"
                     /translation="RINASGSRTAFSEQPIMRTYSGDAPPAEVPTADRNYFSYNINIQ
                     QLLFDFNGTLSKYRASKSLLEAQKLDTKRVRNSLALEFAIAYFDVLEAKKLVGAAEKE
                     VERLESHLKDAEGMYSAGVITKNDLLQARVRLSDAKQRRLTAVNSQDVLTSRLSNLLL
                     IPLDRKLDVVEYAEEVVNPAVFNYEQIWKKALEKRPEIRIADRTIEAVDFEAVAKKSE
                     FLPKVYAKVGNDYMENSYAIHPDNWSFMVGVDINLFEGGSSRADLRKTAYRKQKLVEQ
                     RSKLVDEVKLEVKRYFLDLQNAYERIQVTYDVLDQAQENLRINRGRYEAGVGTATDVL
                     DAVTLLTVAETNHIRSLYDYMKSEAAVAYASGEDLVLVYSR"
BASE COUNT          401 a          685 c          702 g          514 t
ORIGIN      
        1 ccgcaaccct cgcctggtcg agcatcgcgg gggatgcgtc gagccccaca ggatggaatc
       61 cgtggtcggc gaggatgcgg gtgagatccc ctgccccgca ccccaagtcc agaatccgcc
      121 ggtggccgcg ctctttcagg aggccgatca ggtccctgcg gagatcgccg aggaaccagt
      181 tcagcgcttt ccccaggagg gggaaaaagg tgcgatgggt cggtagggat cggacacggc
      241 cttgcctgaa tttagcgtat gccgctcttt cgaacatcag ggcagatgtt cttccggcat
      301 tcgtcacgcc cccgttcgtc ggagaggttt ttctccacgg tactgcttca tggaactgaa
      361 acttattcga atcgcacgtc gtcgaagttg atgtggcccc agccgccgct ggaggcgtct
      421 atgagcctga tctgcgccga ttgcccccga taggccgata cgtcccacgt tattctcccc
      481 atggtctcag tgcacttccc tgttgccctg aggaccgcct ggccgttcac gagaagttcg
      541 acccgttccg tgttgatgtc gcagccgccg ccgatgagaa aactgatgcg gggagatgtg
      601 atcgtgaatg agtcggaggt gagggtccct tgaggcccgt ctccctgaac ccggcccggc
      661 gggtcggatg gccggtgccg gttttcgtaa ccgccgatcc agtagttgcc ctggtggttg
      721 gagggttgcc ctcggtgccg ggcggtgggg ttgtccccgt aggtcggctg gttcgagaag
      781 gcgttacccg tggcgttcca gccgcgtagg tcgccggtct cgaagtccca ggtgatattg
      841 cccgacgccg gcatggccgt ttcttcgaag gagatgccga cgacgtcctc cttgttcacg
      901 gggacctgga cgacccggcc gtccttcaga aagaccgcca tccccgctac ggctgcggag
      961 gccgccgtca tgaccacgat gatagaaagc agagccggcc aaaaactttt atttcctctt
     1021 ctcatccttt atgcctcctt ttgcgttgaa attcttaccg taattaccat agatcgccgt
     1081 aaaaagcact cctccgaagg gatggcgcct gtccggacgg aatgatcaaa atgggggagg
     1141 ggagtccttg cccgccaccg tggacctcag acggtcatct ggaatagacc aggacgaggt
     1201 cttcgcccga tgcgtacgcg acggcggcct ccgatttcat gtaatcgtag agcgagcgga
     1261 tatggtttgt ctccgcgacg gtcaacagcg tgacggcgtc caggacgtcg gtcgcagtcc
     1321 ccacgcccgc ctcatagcgg ccccggttga tgcggaggtt ttcctgtgcc tggtcgagga
     1381 cgtcataggt cacctggatg cgctcatagg cgttctgcag gtccagaaaa taccgcttca
     1441 cttccaactt gacttcgtcc acgagcttcg agcgctgttc gaccagtttc tgtttccggt
     1501 aagccgtctt ccggaggtcc gcccgggagc ttcctccctc gaaaaggttg atgtcgacgc
     1561 ccaccatgaa ggaccagttg tccggatgga ttgcgtaaga gttttccatg taatcattgc
     1621 cgacctttgc gtacaccttg ggcagaaatt ccgacttctt ggccacggcc tcgaagtcga
     1681 cagcctcgat cgtcctgtcg gcgatccgga tctccggcct cttttccagg gcctttttcc
     1741 aaatctgttc gtagttgaag acggcggggt tcacgacctc ttccgcatac tccacgacgt
     1801 ccaatttcct gtcgagcgga atgagcaaca ggttgctcag gcgggacgtc aggacgtcct
     1861 gggagtttac cgccgtcagg cgccgttgtt tcgcgtcgga gagccggaca cgggcctgga
     1921 gcaggtcgtt cttggtgatg accccggcgc tgtacattcc ttcggcatct ttgaggtggg
     1981 actcgaggcg ttcgacctcc ttctcggcgg ccccgacgag tttcttcgcc tcgaggacgt
     2041 cgaagtaggc gatcgcaaat tcgagggcaa ggctgttgcg tacgcgcttg gtgtcgagct
     2101 tctgcgcctc cagtagactc ttgctcgccc ggtacttcga caacgtcccg ttgaaatcga
     2161 acaggagctg ctggatgttt atgttgtacg agaaatagtt ccggtccgcc gtgggaacct
     2221 cggcgggggg agcgtcgccg ctgtacgtgc gcatgatcgg ctgctcggaa aacgccgtcc
     2281 gggaccccga ggcattgatc cg
//
LOCUS       DAOTMX010000281         2301 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_36426_length_2301_cov_3.683882, whole genome shotgun sequence.
ACCESSION   DAOTMX010000281 DAOTMX010000000
VERSION     DAOTMX010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36426_length_2301_cov_3.683882"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="P5573_07645"
     CDS             <1..295
                     /locus_tag="P5573_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRT27972.1"
                     /translation="DAGAYRDFCSGAELLIHDAEFTEEEYRRFRTWGHSSYKSALDLA
                     LRAGVKELGLYHLNQDRTDAEMNEIVVDCRRIIADRGAKLECFGVTSDMSIRL"
     gene            318..1760
                     /locus_tag="P5573_07650"
     CDS             318..1760
                     /locus_tag="P5573_07650"
                     /inference="COORDINATES: protein motif:HMM:NF014510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HRT27973.1"
                     /translation="MFADVISIFHSVRIQDILDIALIAVMAYGLLLWFKKTASRLVLV
                     GIFLLGIVYVLSRFFQLYMTAMILQSVFAVLIFALVVIFQEELRRFFERLALWGRIRK
                     KEYGSSQEHEAEVITQVVGNLSRKHVGALIVIQGEEPLDRHLQGGIRLEGILSQPLLE
                     SIFDPHSIGHDGAVVIDNGRVVQFGAHLPLSSNVREFGNLGLRHTAALGLSERSDAVC
                     IVVSEERGTISVARDGELRILDNAAKLKAVLDDFYEKKASPERHRPALWFRKNSAEKA
                     AAVLLAVLVWVAFGYQRETVQREFVVPIEYRNLAQEWMIEEPKVTEAKVMLKGPEQAF
                     RLFDASKLKISLDLSQVKQGGQEIALTKDMVKTPSSLPVVGIKPERIFITAYRLLPVS
                     LPVEVRTVGTPPPGTTIQKIEVVPPSVQVLMSPRLRREGMTVPTKPIDLSSITTTTTV
                     KPDLLFPPEVRFLGEKPPAVAVIVKAKQKR"
     gene            complement(1836..>2301)
                     /locus_tag="P5573_07655"
     CDS             complement(1836..>2301)
                     /locus_tag="P5573_07655"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRT27974.1"
                     /translation="MFHPSAAVRVKTFLDHAPMAAGVLLVDIGCGDGRVLKAAAKRYD
                     VRAVGFEVNPLAYLLALIRTLGMKSVEIKYGDFWKADLREADVVFCYLFPDVMRRLAV
                     KLETELKPGARVCSCNFPIPGWRPIETLLPESSLHGDPIYLYQFPGSCKPSA"
BASE COUNT          475 a          687 c          691 g          448 t
ORIGIN      
        1 cgacgccggt gcttacaggg atttctgctc cggggccgaa ctcctgatcc acgacgccga
       61 attcaccgaa gaggagtaca gacgtttcag gacgtggggg cattcttcct ataagagtgc
      121 gctcgacctc gccctgcgtg ccggcgtcaa ggaattgggg ttgtaccatc tgaaccagga
      181 ccggacggat gcggagatga acgagatcgt cgtggactgc cgtcggatca tcgccgaccg
      241 gggcgcaaag cttgagtgtt tcggcgtcac gtcggacatg agcataagac tgtaacctgg
      301 gctccgggga gtttgcgttg tttgccgacg tcatatctat tttccactcg gtcaggattc
      361 aagacatatt agacatcgcc ctcatcgccg tgatggcata cgggctgctt ctgtggttca
      421 agaaaacggc ctcccgttta gtccttgtag gtatctttct tctcggcatc gtctacgtgc
      481 tgtcgcgttt cttccagctt tatatgacgg cgatgatcct ccagtcggtg ttcgccgtcc
      541 taatcttcgc cctcgtcgtc atctttcaag aggaactgcg gcggttcttc gagcgcctcg
      601 ccctgtgggg aaggatacgt aagaaagagt acgggtcttc gcaggaacac gaggccgagg
      661 tcatcaccca ggtggtcgga aatctatccc gcaaacacgt cggtgcgctc atcgtgatcc
      721 agggggaaga gccgctcgac aggcacctgc agggagggat ccgactggag gggatactca
      781 gtcagccctt gctcgagagc atcttcgacc cccactcaat cgggcacgac ggcgccgtcg
      841 tcatcgacaa cggcagggtc gtccagttcg gcgcccacct gcccctttcc tcgaacgtcc
      901 gggagtttgg gaacctcggc ctgcggcata cggcggccct cggcctgtcg gaaaggtccg
      961 atgccgtctg catcgtcgtc tccgaggaac gcggtacgat ctccgtggcc agggacggcg
     1021 aactgagaat cctggacaat gcggccaaac tgaaagcggt gctcgacgac ttctatgaaa
     1081 aaaaggcgtc tcccgagcgg caccgccccg ccctctggtt taggaagaac tccgccgaga
     1141 aggcggcggc tgtcctcctc gccgtccttg tctgggtcgc tttcgggtac cagagggaga
     1201 ccgtgcagag ggaattcgtg gtgcccatcg agtaccgtaa ccttgcccag gagtggatga
     1261 tcgaagagcc caaggtcacg gaggccaagg tgatgctcaa agggcccgag caggccttcc
     1321 gcctttttga cgcatcgaag ctgaagatat cgctcgacct atcccaggtc aagcagggcg
     1381 gccaggagat cgctttgacc aaggacatgg tcaagacacc gtccagcctt cccgttgtcg
     1441 ggatcaagcc ggagcggata ttcatcacgg cctaccggct tctgcccgtt tctctccccg
     1501 ttgaggtcag gaccgtgggc accccgccgc cgggtacgac gatccagaag atcgaagttg
     1561 tgcctccttc cgtacaggtc ctcatgtcgc cgagactccg cagagagggg atgacagtgc
     1621 cgacgaagcc gatagacctt tcttcgatca ctacgaccac caccgtcaaa ccggacctcc
     1681 ttttcccgcc cgaggttcgc ttcctgggcg agaagccgcc ggccgtcgcc gtcatcgtta
     1741 aggcaaagca gaagagatga tacgaaacgt ccgtaatctg ccgtaggcaa gggacggtgt
     1801 ctccagtcga tggaaccggg gagtaaaagg aagagctacg cgctgggttt gcaggaaccg
     1861 ggaaactggt acaggtagat cgggtccccg tggagagacg attccgggag cagggtttcg
     1921 atgggcctcc accccgggat gggaaagttg caggagcaga cgcgcgcacc cggcttgagc
     1981 tcggtctcca acttcacggc gaggcgcctc atgacgtcgg gaaaaagata gcagaagaca
     2041 acgtccgctt cccgcaggtc ggctttccag aaatcgccgt atttgatttc aacggacttc
     2101 atgcccagcg tccgtatcaa cgccaggaga taggccaacg ggttcacctc aaagcccacg
     2161 gccctgacgt cataccgctt ggccgccgcc ttcaagacac gaccgtcgcc gcagccgatg
     2221 tcaacaagca ggaccccggc cgccatcggc gcatggtcca ggaaggtctt cactcgaacc
     2281 gccgcagagg ggtggaacat g
//
LOCUS       DAOTMX010000282         2290 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_36659_length_2290_cov_2.944519, whole genome shotgun sequence.
ACCESSION   DAOTMX010000282 DAOTMX010000000
VERSION     DAOTMX010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36659_length_2290_cov_2.944519"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="P5573_07660"
     CDS             complement(<1..430)
                     /locus_tag="P5573_07660"
                     /inference="COORDINATES: protein motif:HMM:NF013242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27975.1"
                     /translation="MSKIKIATDWLAGCAGCHMSLLDIDERIVKLLESVEFTSSPVTD
                     LKHPPEDGVTIGILEGAICNTHNIEVAREMRKRCQILIAIGDCATFGGVPAMRNLVGT
                     QEALKRAYIETESVVGGFIPGSSELGEPLDTVTGVDKVVKV"
     gene            complement(423..2228)
                     /locus_tag="P5573_07665"
     CDS             complement(423..2228)
                     /locus_tag="P5573_07665"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRT27976.1"
                     /translation="MSQVIVTIDNIEVKVRPGTTILAAAKEAGIDIPTLCNHRALSPT
                     GACRVCVVEVEGQRTLQAACVFPVAEGMKIKTDSDRVVKARKLVLDMIFAERNHFCPF
                     CEASGDCELQNLGYRYGIDHWAYETYSKPYPLDATREFFLMDHNRCILCGRCIRACDE
                     LVGNHTLGLQQRGTRSMVGADTNTPFGESTCVSCGTCLQVCPTGAIFDGRGSYMGREV
                     QVEHIRSTCSQCSIGCGIEIATRGGNVVRVKGDWDAEVNKGLLCRKGRFERLYDGRKR
                     VKSPLLRQGGRLQEVSWEEALKATAERLKDADAGGVGVLTSSNATNEALFMLSALLGE
                     MKVTNIGLLNDASAGLSGIRRGALADIAKGDVILVVGADPVMNQPVASFLIKRAVDKG
                     ARLIVVDDADNDLACLATMKFGVPEVRKAVDVAARAENPVVIYGERVPPPAVGALEAL
                     RQRAVFIALEPGVNTQAAVAFGLKNGFKASAVKTLFVLFGEEQWDGKGVELGEIGKDA
                     FVAVVSSYISPLTDRADVVLPMAIWSERSGTITNVEGRVQKVCAAVDPEGEAKPDWEI
                     ISLLARMLGKKIDESPDKMSDRAIEALKGGGIHRE"
BASE COUNT          483 a          728 c          562 g          517 t
ORIGIN      
        1 ccaccttgac cactttgtcc acccccgtca ccgtatccag gggctcaccg agttcagagg
       61 aaccgggtat gaagccgccg acaacactct ccgtctcgat gtacgcacgc ttcaacgcct
      121 cttgtgttcc cacaagattg cgcatagcgg gcacaccccc gaaggtggcg caatctccga
      181 tggcgatcag tatctgacaa cgcttgcgca tctccctggc tacttctatg ttatgcgtgt
      241 tgcatatcgc cccttcaaga atacctatcg tcactccatc ctccggcggg tgtttcaggt
      301 ccgtcacggg gctggaagta aattcgaccg attcgagcag cttgacgatc ctctcatcga
      361 tatccagcaa ggacatgtga caacctgcac agccggccag ccagtccgta gctatcttta
      421 tcttactcac gatggattcc ccctcccttc aatgcttcga ttgcccgatc ggacatctta
      481 tccggtgatt catcaatttt cttccccagc atgcgggcga gcaaagagat tatctcccag
      541 tcgggcttgg cttcaccttc agggtccacg gccgcgcaga ccttctgaac acgaccctcg
      601 acattcgtta tagtcccgga cctttccgac catatggcca taggcaggac aacgtcagcc
      661 ctatcggtaa gcggtgaaat gtagctggag acgacggcca cgaaggcatc cttaccgatt
      721 tctcccagct caacgccctt gccgtcccat tgttcttcac cgaaaaggac gaacaacgtt
      781 ttaacggcgg aggccttaaa accgttcttc aacccaaaag ccaccgcagc ctgtgtattg
      841 acgcccggtt ccagggcgat aaaaacagct ctttgtcgca gtgcctcaag ggcgccgacg
      901 gcgggtggcg gcactctttc cccgtagata acgacggggt tttccgcgcg ggcggcgaca
      961 tccacggcct tccggacctc cggcacgccg aatttcattg tagctaaaca agcgaggtca
     1021 ttgtctgcgt cgtcgacgac gatcagccgg gcccctttat cgacggcccg tttgatgaga
     1081 aatgacgcaa cgggctgatt catgaccggg tctgcaccga cgaccagtat gacatcccct
     1141 ttcgctatgt cggcgagtgc gcctctccgt atacccgaca gtcctgcgga agcatcgttc
     1201 aacaagccga tgttggttac tttcatttct ccgagcagcg cagaaagcat gaacagggcc
     1261 tcgttagtag cgttactcga cgtcaggact ccgacacctc cggcatcggc gtctttaaga
     1321 cgctcggcgg tcgctttaag ggcctcttcc cagcttactt cctgaagcct gcccccttgc
     1381 ctcaacaggg gggactttac ccgtttgcgc ccgtcgtaca gcctttcaaa gcgacccttc
     1441 ctgcacagca agcccttatt gacttctgca tcccagtcac ccttgacccg cacgacattg
     1501 ccgccccgcg tcgcgatttc gatgccgcat cctatactac attgactaca ggtactcctg
     1561 atgtgttcga cctggacctc gcgccccata taggaacctc ggccgtcgaa tatggcgccc
     1621 gtcgggcaca cctgcaggca ggtaccgcag gacacgcaag tcgattcccc gaagggggtg
     1681 ttggtgtcgg cgccgaccat ggaccttgtg ccccgctgct gtaagccaag ggtgtgattg
     1741 cccacaagct cgtcacaggc acgaatacaa cgcccgcaga ggatgcaacg attgtgatcc
     1801 atcagaaaaa actcgcgggt ggcgtcaagg gggtatggtt tcgagtaagt ctcgtaagcc
     1861 caatgatcga tcccgtaccg gtatccaaga ttctgcaact cacagtcgcc gcttgcctcg
     1921 cagaatgggc agaaatggtt tcgttcagcg aatatcatgt cgagaaccag ttttcgcgcc
     1981 ttgaccaccc ggtccgagtc cgtctttatt ttcatcccct cggctacagg gaaaacacag
     2041 gcggcctgca aggtccgctg cccttcgacc tctacaacgc aaacccgaca ggcaccggtc
     2101 ggggacagcg ctcggtggtt gcaaagtgtg gggatatcta tccccgcctc tttcgctgcc
     2161 gctaaaatcg tcgttccggg tctaaccttc acttcgatgt tgtcgatcgt cacaattacc
     2221 tgagacatgc accttcctcc attcacatct gtggttaagt cgccactcca aagagaatct
     2281 tacatcatat
//
LOCUS       DAOTMX010000283         2288 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_36694_length_2288_cov_3.793103, whole genome shotgun sequence.
ACCESSION   DAOTMX010000283 DAOTMX010000000
VERSION     DAOTMX010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36694_length_2288_cov_3.793103"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(184..888)
                     /locus_tag="P5573_07670"
     CDS             complement(184..888)
                     /locus_tag="P5573_07670"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HRT27977.1"
                     /translation="MEFMSQKKHSLDKIIEEQIKRWQIDQKKRYKKPIRPVITMSRLP
                     GAGGDSIARRVAEDLQIDLFDTEIVSRIAESSKVSKQVVESLDEQDRSIFNDWLKFLD
                     KDHLWSDEYLQHLTRVVSAIGTHGHAFIVGRGAGFILPEEVCLRLLVVAPLQTRIDNV
                     VKAFGVSEEEARKRLLKSESARKAFIRKYFNKDMLDPLNYDLVLNTKNLSIDAASNII
                     KEAFNSREWYNYNVRK"
     gene            complement(1040..1627)
                     /locus_tag="P5573_07675"
     CDS             complement(1040..1627)
                     /locus_tag="P5573_07675"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HRT27978.1"
                     /translation="MKAVITLPPVDPKGATSLEEVLGTRESWRRFSSEPLTNDEVARI
                     MWAAQGITRAWGGRTAPSAGALFPLEIYVVLEGGVFHYIPRNHHLLCLSGEDVREALS
                     SAALSQAFIREAPATVVIAVVFERVTVKYGGRGERYAMIEVGHAAQNILLQATSMGLG
                     SVPVGAFHDERVRQVLDLPDDHRPAYLVTVGHRRK"
     gene            1710..>2288
                     /locus_tag="P5573_07680"
     CDS             1710..>2288
                     /locus_tag="P5573_07680"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRT27979.1"
                     /translation="MEVNKEIALYLEDSFPLEMKPAEDTFEPEPWATNPHLQTIFGSL
                     SLRTLGRNAMLEQSREMIVEGGNGVRLLGYLSRRLGEESPGLVILIHGWEGSSESAYI
                     LSTGRYLFERGFDVFRLNLRDHGRSHHLNDGLFHGALTDEVLTAAVEISRSARRNSFI
                     VGFSLGGNFALGIALRAPQAGIGNLKQVVCISP"
BASE COUNT          494 a          636 c          602 g          556 t
ORIGIN      
        1 gttcgatatc cagaaaagga ccatcgccgg gctctgcgaa ctgaagatga ctttcctgag
       61 agattgaggc cgatatacaa gggccgagtc aaacgcaaga aacaacaaag aggccgctgc
      121 agtttatagc gcggcctctt cttttcactc gaaatatcga acccttgaat ctccttgctc
      181 tccctatttc cttacattgt agttgtacca ctcccgtgaa ttgaaggcct cttttatgat
      241 attcgaggct gcgtcgatgc tcaggttctt cgtgttcaga accaggtcgt agttgagcgg
      301 gtcgagcata tccttattga aatacttccg gatgaatgcc ttgcgggccg attctgactt
      361 tagcagccgc ttcctggctt cttcctcgga gacaccgaag gccttcacaa cattgtctat
      421 cctcgtctgc aatggggcaa cgacaagcaa tcgcaggcac acctcctccg gcaagatgaa
      481 gccggcaccc cggccgacga tgaatgcgtg accgtgagtc ccgatggcac tcacgaccct
      541 cgtcaggtgc tgtaggtact cgtcagacca cagatgatcc ttgtcgagga atttcagcca
      601 gtcgttgaaa atcgagcgat cctgttcatc cagcgattcg acgacctgct tgctgacttt
      661 cgaactctcc gctatcctcg agacgatttc agtgtcgaag aggtcgatct gtagatcctc
      721 tgcaacccgg cgtgcgatgg aatcccctcc tgcaccaggc agccgcgaca tggtgatgac
      781 gggccggatg ggctttttgt atctcttctt ctggtcgatc tgccatctct tgatttgctc
      841 ttctatgatt ttgtcaagcg aatgtttttt ctgcgacata aattccatgg gtttcctcct
      901 ctgcgatttg ttgatcctcc gccctgtggt tcgccgtggt gcatttagaa tactttagaa
      961 tactaaagaa tcggcgacta ttttgcaaca taaatacgga gggacgaaaa ggcgtgcctt
     1021 ccgttatgat gtggtttgct tactttcgcc tgtgtccgac cgtaacaagg taggcaggtc
     1081 tatggtcatc aggaaggtca aggacctgac gaacgcgttc atcgtggaaa gccccgaccg
     1141 gcacggagcc gagtcccatg gaggtcgcct gaagcaggat gttctgtgcg gcgtggccca
     1201 cctcgatcat ggcataacgc tcaccacgac cgccgtattt taccgttact ctttcaaaca
     1261 cgacagcaat tacgaccgtt gccggggcct ctcggatgaa tgcctgtgat agcgcggcag
     1321 aagaaagtgc ttccctgacg tcttcacccg agagacacaa aagatggtgg tttctcggga
     1381 tgtaatgaaa gacaccacct tcgagcacaa cgtatatttc cagaggaaaa agcgcaccgg
     1441 cggaaggggc tgttcggccg ccccatgctc tcgtgatgcc ctgggccgcc cacataatgc
     1501 gggcgacctc gtcattagtc agcggctccg aggaaaaacg tcgccaggac tctcttgtcc
     1561 cgaggacttc ttccagggaa gttgctcctt tggggtcgac aggcggcaat gtgatgacag
     1621 ccttcatgtt ttcccggtcc tgcatcgtcg ttaacccaga gctattcgca tgaaggtcac
     1681 ttgatgtctg attttagctg tgttattttt tggaagtcaa taaggaaata gcgctgtacc
     1741 tggaggattc tttcccactc gagatgaaac ccgccgagga tacattcgaa cccgaaccct
     1801 gggctaccaa cccccacctt cagactatct tcggaagcct gtcattgcgg actctgggga
     1861 ggaacgccat gctggaacag tcccgggaga tgatcgtgga gggcggcaac ggtgtccgac
     1921 tcctgggtta cctctccagg aggctgggcg aggaatcccc tgggctggtc atccttatcc
     1981 acggctggga aggtagttcg gaatccgcct acatactctc tacgggccgt tatctcttcg
     2041 agcgcggctt cgacgttttc agactcaacc tcagggacca cggccggagt caccatctga
     2101 acgatggcct ttttcacggg gccttgaccg acgaagtcct cactgccgcc gtcgaaatat
     2161 cccggtcggc ccggagaaac tcattcatcg ttgggttctc actcggaggg aacttcgccc
     2221 tgggcatcgc cttgagggca ccccaggcgg ggatcggcaa cctgaaacag gtggtctgta
     2281 taagcccc
//
LOCUS       DAOTMX010000284         2274 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_37013_length_2274_cov_3.048220, whole genome shotgun sequence.
ACCESSION   DAOTMX010000284 DAOTMX010000000
VERSION     DAOTMX010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37013_length_2274_cov_3.048220"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="P5573_07685"
     CDS             <1..269
                     /locus_tag="P5573_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27980.1"
                     /translation="HSGIVNTALYAREVTGVDRVHVIMGGFHLSGPAFEPIIGRTTEE
                     LKKINPAYIVPTHCTGRRAIMHMEQAMPGKFILNMSGTRLTFSA"
     gene            complement(475..1656)
                     /locus_tag="P5573_07690"
     CDS             complement(475..1656)
                     /locus_tag="P5573_07690"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRT27981.1"
                     /translation="MLCRLLFTPEKVLVLEQDGGGAASKDPINWKYLIETAHREGVAA
                     LLHYNLRRYGLGHLLPENLESELTDYYYRRVKTTLESIVVLREVFRGIQAAGIDFLVF
                     KGIALGECVYPQTALRGAGDCDLLVKKRDLLTVDNYINSLGYRPVDTSARLALDNPGG
                     YLASVEYRRDDPSSVSIHVHWHLVNTSVPATAYITFIDMDDLWEKSHMAKVADTDTRI
                     LSPEHLIIYLCEHALRVGHSFDRLILVCDLFFALRAYEKNIDWGSLVDVARRFHLDRF
                     VYFGLLITSRFSAFPLPSGVMEKLKPAKITLPECWFLNTQLANDRIRGSSFFVHLAMN
                     ERPLDKARFVISTFFPPRSILFQRRYTTEDVAGARLYLDRAREIGRQFFLVLRAGRRK
                     R"
     gene            1795..2085
                     /locus_tag="P5573_07695"
     CDS             1795..2085
                     /locus_tag="P5573_07695"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HRT27982.1"
                     /translation="MIADDERLVQNENSVFREEDDGAFLFDPEGGRLKYLNTTGAEVY
                     RLCDGTRNVREIVDVVAGVFAETPRNDIERDVTAFLATLLEMNFLVRARHGT"
     gene            2075..>2274
                     /locus_tag="P5573_07700"
     CDS             2075..>2274
                     /locus_tag="P5573_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27983.1"
                     /translation="MVPEETKHFPLHSIYLYLSDRCNLRCRHCWVAPSDTQGPPARRN
                     DVDVSFIKEAVVKAKALGLNTV"
BASE COUNT          534 a          607 c          636 g          497 t
ORIGIN      
        1 cccattcggg gatcgtcaac accgctcttt atgcccgaga ggtgacgggt gtcgatcgag
       61 tccacgtcat catggggggc tttcatctgt ccggcccggc cttcgagcct atcatcggcc
      121 ggacgacgga ggaactgaaa aagatcaacc cggcttacat cgtcccgacc cactgcaccg
      181 ggagaagggc gatcatgcac atggaacagg cgatgccggg taagttcatc ctgaacatgt
      241 cagggacgag actgactttt tctgcttagg atgtcattgc ttccaaataa gctgtgtccc
      301 taaaaactca gaaagtcatg cccggcccaa ctgagctatc ccttccatcc tactgagccg
      361 aaggagaagc ggtttcgaac ttcttgagac cctccagaaa ggtctcaggg tgcggtatct
      421 ttttatcttt attgagaatt gttggaatgg cagtgaaaaa cgtctgtttg gggtctatct
      481 cttccttcgc cctgctctga ggacgagaaa aaattgtctg cctatctccc gagcacggtc
      541 caggtacaac ctcgcgccgg cgacgtcctc cgtcgtgtaa cgcctctgaa acaggatgct
      601 ccgtggggga aaaaaggtgg atattacgaa ccttgccttg tcgaggggac gttcgttcat
      661 tgccaggtga acgaaaaaac tgctcccccg aatcctgtca ttggcaagct gagtattcaa
      721 gaaccagcac tcaggcagtg tgatttttgc aggtttaagc ttctccatga cccccgaggg
      781 caagggaaag gcgctgaagc gggacgtgat gagaaggccg aagtagacga aacggtcaag
      841 atggaacctc cttgccacat caacgagcga accccaatcg atgttcttct cgtaagccct
      901 gagggcgaaa aagaggtcgc agacgaggat gaggcggtcg aaggagtggc cgacgcggag
      961 ggcatgttca cagaggtaga tgatcaggtg ctccggggac aggattcgcg tatccgtatc
     1021 tgccaccttg gccatgtggg acttctccca caggtcatcc atatctatga aggtgatgta
     1081 ggccgtcgcc ggcacggacg tattcaccag gtgccagtga acgtggatgc ttacagacga
     1141 ggggtcgtcc cgccggtact ctacgctggc caggtatccc ccggggttgt cgagggccag
     1201 gcgggcgctg gtgtcgacgg gccggtagcc gaggctgttg atgtaattgt cgacggttag
     1261 gagatcccgt tttttcacga gcaggtcgca atcgcccgcc ccgcgcaaag ccgtctgggg
     1321 gtaaacgcac tcccccaggg cgatgccctt gaaaaccagg aagtcgatcc ccgccgcttg
     1381 gataccacgg aatacctccc ttagaacgac gatagattct aaagtagtct ttactcgacg
     1441 gtagtagtaa tccgtcaact cactttcaag attttcgggc aagagatgac caagcccata
     1501 acggcgcaag ttgtagtgaa gcaaggcggc gacaccttct cggtgggctg tctcgataag
     1561 gtatttccag tttattgggt ctttggaggc cgcccccccg ccatcctgct ccagcaccag
     1621 caccttctcc ggtgtgaaca ggagccggca gagcaacctg tcttcgtttc tccaggctat
     1681 ggtctcctcc cgtcgggccg ccccgtatag aatcgaaaaa tatttaaatt tcctatgacc
     1741 tgtattatag tcatcacatt gaataagtca aaaagtgggg gatagccagc gccgatgatc
     1801 gcggatgatg aaagacttgt ccagaacgaa aacagcgtct ttcgggaaga ggatgacggt
     1861 gccttcctct tcgacccgga agggggaagg ctcaagtatc taaacaccac aggggccgag
     1921 gtataccgcc tgtgtgacgg taccaggaac gtaagggaga tagtagatgt cgtggccggc
     1981 gtttttgcgg aaacaccgag aaacgacatc gaaagggacg tcacggcctt cctcgccacc
     2041 cttctcgaga tgaacttcct tgtccgggct cgtcatggta cctgaagaaa caaaacactt
     2101 tccattgcat tccatatacc tgtacctgtc ggaccgttgt aacctccgct gccgtcactg
     2161 ctgggtggct ccgtccgaca cgcagggtcc gcctgcaagg agaaatgatg tggatgtctc
     2221 ctttatcaag gaggccgttg tcaaggcaaa agcactgggt ctcaacacag tgaa
//
LOCUS       DAOTMX010000285         2267 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_37160_length_2267_cov_3.533002, whole genome shotgun sequence.
ACCESSION   DAOTMX010000285 DAOTMX010000000
VERSION     DAOTMX010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37160_length_2267_cov_3.533002"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1330
                     /gene="uvrA"
                     /locus_tag="P5573_07705"
     CDS             <1..1330
                     /gene="uvrA"
                     /locus_tag="P5573_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRT27984.1"
                     /translation="ATQIGSGLMGVLYVLDEPTIGLHPRDDARLISTLKRLRDMGNAV
                     LVVEHDPEMMLSSDYIVDMGPGAGLKGGEVIFQGTPEEICESPESLTGAYLSGRMKIP
                     VPAARRAATGRHICLEGATENNLKDIDIKIPTGVMTCVTGVSGSGKSTMVIDTLYKVI
                     ARRLYRCRDRAGKVRKVRDLGGIERVIVMDQQPIGRTPRSNPATYTGAFNAIRDLFAQ
                     LPDARVRGYHSGRFSFNAKGGRCEACKGDGVIKIEMHFLPDVYVTCDVCHGKRFNRDT
                     LDILYKGKNIADILDMTVSQALSFFENVPAILSKLALLNDVGLGYIKLGQSATTLSGG
                     EAQRIKLARELGKRVKGNTLYILDEPTIGLHFADVQKLLDVLARLVDYGNTVVVIEHN
                     LDVIKSADYVIDLGPEGGPGGGRIIASGTPEDVAMEEASLTGQFLRRVLQ"
     gene            complement(1364..1627)
                     /locus_tag="P5573_07710"
     CDS             complement(1364..1627)
                     /locus_tag="P5573_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27985.1"
                     /translation="MEKMCKVVGIIVALLIVVVFYWTRYQVIPVNYAYAPGFYKVNRL
                     TGEVNLVLGKEYVTVEKAVDRGPRPEAAPTPETAPAPAPSPAK"
     gene            complement(1881..>2267)
                     /locus_tag="P5573_07715"
     CDS             complement(1881..>2267)
                     /locus_tag="P5573_07715"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27986.1"
                     /translation="HFLDARGRDLDLFFILGRDAFADIRRWKDWEKLLLLCHFVVMTR
                     PGYDDQRLDEILPTSTAASFRYLPARKAFRSPSGSSLFFRSVTFLDISSTDIRKRLSR
                     GKSVRYLVPDAVTGYIDRHSLYRRRA"
BASE COUNT          482 a          628 c          713 g          444 t
ORIGIN      
        1 ggctacgcag atcgggtccg ggctgatggg ggttctttat gtcctggacg aacccactat
       61 cggcctccac cccagggatg acgcacggct gatctctacg ttgaagcgcc tccgggacat
      121 gggtaatgcc gttctcgtcg tcgaacacga ccctgaaatg atgctttcgt ccgactacat
      181 cgtcgacatg ggtcccggcg ccggcctgaa ggggggagag gtcatctttc agggcacgcc
      241 ggaggaaata tgcgaatctc cggaatccct gacgggcgct tacctttccg gcaggatgaa
      301 gatccccgtg cccgccgcga ggcgcgccgc tacggggagg cacatctgtc tcgaaggggc
      361 gacggagaac aacctcaagg acatcgatat caagattccc acgggcgtga tgacctgcgt
      421 caccggggtg tcggggtcgg gaaagagcac gatggtcatc gacaccctgt acaaggtaat
      481 cgcccgcagg ctttaccggt gccgcgacag ggcggggaag gtcaggaagg tgagggacct
      541 ggggggaatc gagcgggtga tagttatgga tcagcagccc atcggccgaa cgccccggtc
      601 gaacccggcg acgtacacgg gcgcattcaa cgcgatccgc gacctattcg cccagcttcc
      661 ggacgcgagg gtgaggggtt atcattccgg ccgcttcagt ttcaacgcaa aaggcggcag
      721 gtgcgaggcc tgcaagggcg acggcgtgat aaagatcgag atgcatttcc tccccgatgt
      781 gtacgttacc tgcgacgtat gtcacgggaa gcgtttcaac cgcgacacac tggacatcct
      841 ctataagggg aaaaacatcg ccgacatcct ggatatgacg gtgagtcagg cgctctcttt
      901 ttttgagaat gtccccgcca tcctgtccaa gcttgcgctc ctgaacgacg tcggtctcgg
      961 ttacataaag ctgggacagt cggcgacgac gctctcgggc ggcgaggcgc agaggataaa
     1021 acttgccagg gaactgggca agcgcgtgaa gggcaacacc ttgtacatcc tggacgagcc
     1081 gaccatcggg ctgcactttg ccgacgtcca gaagctcttg gacgtcctcg cgagactcgt
     1141 cgattacggc aacaccgtcg ttgtgatcga acacaacctg gacgtcatca agtcggccga
     1201 ctacgtcatc gacctcggac ccgagggtgg gccgggagga gggcgcataa tcgcctccgg
     1261 taccccggaa gacgttgcga tggaggaagc atcactgacc gggcaattcc tccgcagggt
     1321 cttgcaatga ggtcgaagtg aatatctttt tctgcccgcc tctttacttg gcgggcgagg
     1381 gggcaggagc cggtgccgtt tccggagtcg gcgccgcctc gggcctaggt cctctatcta
     1441 ccgccttttc cacggtcacg tattccttcc ccaggacgag gttgacctca ccggtcagcc
     1501 tgttcacttt atagaagccg ggtgcatagg catagttgac ggggatgacc tggtacctgg
     1561 tccagtaaaa aacgacgacg atgagtagtg cgacgattat tccgacaacc ttacacattt
     1621 tctccattgg tatgacctcc tgcatctatc gattggctaa gggatttaga ctctaagctt
     1681 ggtgctactt gagcgggatc cggcaagcgc ggggtcggct cttgatgacc cctccgaagc
     1741 taaaaaagtt cttcaccgaa gatggacttt cagccggggc gccgcgaaag gaagccgccg
     1801 cctcggttcg gtcgttttgg tcgcgcagta taggagacct tgccgggata cgtcaaggat
     1861 aaagtaacga caaacacatc tcaggcgcgg cgccggtaga gggaatgcct gtcgatgtat
     1921 ccggtgacgg cgtctggtac gaggtaacgg acggactttc cccgcgaaag tcttttcctg
     1981 atgtccgtcg aagagatgtc gagaaaggtg acggagcgga agaagagcga ggatccgctc
     2041 gggctgcgga aggccttccg ggccggtagg taacgaaaac tcgcggctgt ggaggtcggt
     2101 aggatttcgt cgaggcgctg gtcgtcgtaa ccgggccttg tcatgaccac gaagtggcag
     2161 aggagtaaga gcttctccca gtccttccag cgccggatgt ccgcaaaggc gtcccggccc
     2221 aggatgaaaa aaagatcgag gtctcttccc cgggcgtcga gaaaatg
//
LOCUS       DAOTMX010000286         2267 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_37165_length_2267_cov_3.246835, whole genome shotgun sequence.
ACCESSION   DAOTMX010000286 DAOTMX010000000
VERSION     DAOTMX010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37165_length_2267_cov_3.246835"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1200
                     /locus_tag="P5573_07720"
     CDS             <1..1200
                     /locus_tag="P5573_07720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRT27987.1"
                     /translation="GFGILVSWDAAFLLTARDFFSIFGSYNLTVIPLFVFMGQLAHHS
                     GISGRLFNAANKFMGHLPGGLAIATVGACAGFSAICGSTSATAATMASVALPEMKKYN
                     YDPALATGVVAAGGSLGILIPPSTIFIVYGIMTEQSIGKLFMAGVLPGILLAVLFIVT
                     IIIWTKLRPEMVTLAPKSSWKEKIASLSGVIETLTLFVLVMGGLFVGIFTPTEAAAIG
                     AFGTLTIALINRNLAWKGFLQSLYETTRISCMIMVIVAGATVFGHFLAVTGIPTNIGE
                     WAAGLLVPHQVVMAIIILIYLVMGCLMDSLAMIMLTIPIFFPVVMSLGFDPVWFGVII
                     VLVTEMGVVTPPVGINVYVVGGVARDVPLHIIFRGTAHLLLAQLVTALLLILFPGIAL
                     YLPSLIY"
     gene            1257..1739
                     /locus_tag="P5573_07725"
     CDS             1257..1739
                     /locus_tag="P5573_07725"
                     /inference="COORDINATES: protein motif:HMM:NF018437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HRT27988.1"
                     /translation="MNTQQMPISNRCDGYRGLLLFIVILTVVVHGCATLPKEKGKKRE
                     VKRMLVTAYSADQKSTGWKHKWGCFLLPPVYAYGPNKGKPKKVGITSDGTRAKKGTIA
                     ADISKYPYGTKMYVPGYGWGEVHDTGSAIKGDHIDVFFPDVKDARAWGNKYLDVIILR
                     "
     gene            complement(1745..2266)
                     /gene="lspA"
                     /locus_tag="P5573_07730"
     CDS             complement(1745..2266)
                     /gene="lspA"
                     /locus_tag="P5573_07730"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HRT27989.1"
                     /translation="MSCQPVSGGAPLTWRSRPLFFGVAALVVLLDQVTKYIVSTGMPL
                     YSSIPVLDGLFSITYVRNPGAAFGFLATAPVYFRAVFFITVTVAAIILILYYLRRYGF
                     GDWKLTLALSLIMGGAVGNLVDRIRSGEVVDFFDVYIGPYHWPAFNVADSAISVGAVL
                     LLIGMVGKTREGR"
BASE COUNT          503 a          667 c          621 g          476 t
ORIGIN      
        1 gggttcggca tcctagtctc atgggatgcc gcttttctgc tgaccgcccg ggacttcttc
       61 tccatcttcg ggtcatacaa cctgacggtg ataccccttt tcgtcttcat gggccagctc
      121 gcccatcatt cggggataag cggcaggctt ttcaacgcag ccaacaagtt catgggccac
      181 cttccgggtg ggttggccat agccaccgtg ggcgcctgcg ccggtttctc cgccatctgc
      241 gggtcgacga gtgccacggc tgcgaccatg gcctccgtag cgctgccgga gatgaaaaag
      301 tacaattacg acccggctct cgcaacggga gtcgttgccg cgggaggcag tctcggcatc
      361 ctcataccgc cgagcaccat cttcatcgtc tacggaatca tgacggagca atcgatcggc
      421 aaactcttca tggccggggt cctccccggc atcctactgg ccgtcctctt catagtcacg
      481 atcatcatct ggacgaagct gcgcccggag atggtcaccc ttgccccgaa gtcgagctgg
      541 aaagaaaaaa tcgcgtcact ctcgggcgtg atcgaaacac tgaccctttt cgtcctggtc
      601 atgggcggcc tcttcgtggg catcttcacg cccacggagg cggccgccat cggtgccttc
      661 gggactttga caatcgccct gatcaaccgc aacctggcat ggaaaggttt tcttcaatcg
      721 ctttacgaaa ccacacggat atcctgcatg atcatggtga ttgtcgcagg ggccacagtg
      781 ttcggacact tccttgcggt aacgggaatt ccgacgaaca tcggcgaatg ggctgccggc
      841 ctgctggtgc cccatcaggt cgttatggcc atcataatcc tgatttacct ggtcatggga
      901 tgtcttatgg actccctcgc catgataatg ctgacgatac ctatcttctt ccccgtcgtc
      961 atgagtctcg gtttcgaccc ggtctggttc ggcgtcatca tcgttttagt tacggagatg
     1021 ggggtggtta ctcccccggt gggcatcaac gtttacgtcg tcggcggcgt cgcgcgcgac
     1081 gtacccctcc acatcatctt ccgcggcacc gcgcatctac tcctcgccca gttggtaacg
     1141 gcccttctcc ttatcctatt ccccgggata gctttgtacc tccccagcct catctattag
     1201 caggcataag aaggcggacg gttaaccgag acctgttcac atgacaaaaa gcagacatga
     1261 atacgcaaca gatgccgatc agcaataggt gcgatggtta caggggatta cttctcttta
     1321 tcgtcatcct cactgtagtc gtccatggtt gcgcaaccct tcccaaagag aagggcaaga
     1381 aacgggaagt caagaggatg ctcgtgacgg cctactcggc ggaccagaaa tcaacggggt
     1441 ggaaacacaa gtggggctgt ttcctcttac cgccggtgta tgcttacggt cccaataaag
     1501 ggaaacccaa gaaagtcggc atcacctccg acggcacgag ggcgaaaaaa gggacgatcg
     1561 cagcggacat aagcaagtac ccttacggca ccaagatgta cgtcccgggc tatggatggg
     1621 gggaggttca cgacaccggt tcggcgatca agggggatca catcgacgtc ttttttcccg
     1681 acgtcaagga cgcccgggcc tggggtaaca agtacctgga cgtgatcatc ctacggtgat
     1741 cccgtcaccg cccttctctt gttttgccga ccatcccgat gagtaagaga acggcaccga
     1801 ccgatatggc ggagtcggct acattgaagg ccggccagtg gtaaggaccg atatagacgt
     1861 cgaagaaatc gaccacttca ccggaacgaa tccggtcgac gaggtttccc accgcccctc
     1921 ccatgatgag ggatagggcg agagtcagtt tccagtcgcc gaacccgtac ctccgaagat
     1981 agtaaagaat caggattatc gcggcgacgg tcacggtgat aaaaaaaaca gcccgaaaat
     2041 aaacaggcgc cgtcgctaaa aacccgaacg ccgccccggg atttctgacg taggttatac
     2101 tgaacaggcc gtcgagcacc ggtatcgagc tgtagagcgg catgccggtc gaaacgatat
     2161 atttcgtcac ctggtcgaga aggacaacga gtgcggccac gccgaaaaat aaaggccgac
     2221 ttctccaggt caacggggcg cctccgctga caggttggca agacagc
//
LOCUS       DAOTMX010000287         2259 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_37319_length_2259_cov_3.455535, whole genome shotgun sequence.
ACCESSION   DAOTMX010000287 DAOTMX010000000
VERSION     DAOTMX010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37319_length_2259_cov_3.455535"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="P5573_07735"
     CDS             <1..303
                     /locus_tag="P5573_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRT27990.1"
                     /translation="AYSEALVRLLGFRMRDLGLTEEDGFRNIDKIRAFSGPTLIIHAE
                     FDHILPFSEGQALYDASPAADKRLLKIPGANHNDIIMKGFREYIDAVREYAEKAKG"
     gene            complement(329..625)
                     /locus_tag="P5573_07740"
     CDS             complement(329..625)
                     /locus_tag="P5573_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27991.1"
                     /translation="MRKSAFAIAVLVFLACSTAAADTVVYDNDNNLLYRIRNDTVFDR
                     DGNKVGYLRGSRIYDLDKNPLYRVRGSRLYDDRGNAVGKKREASPYGQRGKGKY"
     gene            complement(741..>2259)
                     /locus_tag="P5573_07745"
     CDS             complement(741..>2259)
                     /locus_tag="P5573_07745"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HRT27992.1"
                     /translation="IASCCTLVIELVAGRIMAPFVGVSLYTWTSIIGVVLAGISIGAY
                     VGGVIADRYPRASTLGWLLFLSGLGALAISPLTNLIGAAKFPTTLMVRILILTTFIFF
                     LPSCILGMISPVVVKLTLNNLSETGNVVGKIYAFSTLGSIIGTFATGFFLISWMGTRN
                     ILLSVAVILILSALVFGGAFNRKRSVLVVIAFLAPLVLAFYGYAFKPPLDKDIYYLYK
                     ESDYYTLKLSKYHTKDGQRAEALILDNLIHSINVLDNPAYLDYEYLRIYDEFVTWRMR
                     DVKSLKTLFVGGGGYTYPRYLEVKNPAAEVDVVEIDPEITRIAHEYLGIPKDTRIRSF
                     NEDARWFVMNCQEKGKYDFIFGDAFNDLSIPYHLTTREFAAQLAALLKPDGILLANLI
                     DNYEKGLFLPSYIRTLEEVFGKGHVNLVAINPSYEGISTWVVVASRKKIDFDDFIGFT
                     RNERGFQMRSHVVAQERLQEHLAKRPHVILTDDYAPVDNLTAPIFEERFGYKKNR"
BASE COUNT          489 a          604 c          688 g          478 t
ORIGIN      
        1 gcctactccg aggcgttggt caggctcctc ggcttccgga tgagggacct gggactgacg
       61 gaagaagacg gttttcgcaa catcgacaag atccgtgcct tcagcggacc gacgctgata
      121 atccatgcgg aattcgacca tatcctcccc ttcagcgaag ggcaggcgct ctacgacgcc
      181 tcacccgctg cagacaagag gctcctgaag atccccgggg ccaaccacaa cgacatcatc
      241 atgaaagggt tcagggaata catcgacgcc gtgagggagt acgcagaaaa ggcgaagggc
      301 tgaccgccga ccccgcatca gcgcctaatc agtattttcc ttttccccgt tggccgtaag
      361 gggacgcctc gcgttttttc cccacggcgt tgcccctgtc gtcgtagaga cgggagcccc
      421 tgacccggta aagggggttt ttgtcgaggt cgtagatcct cgaacccctc aggtacccta
      481 ctttgttccc gtccctgtcg aagaccgtgt cgttcctgat gcggtaaagg agattattgt
      541 cgttgtcgta gaccaccgta tcggcggcgg ctgtcgaaca ggcaaggaac actagaacgg
      601 cgattgcgaa ggccgacttt ctcaaaccaa tccctccttg gaggtcatcg ttttttgttc
      661 gtcaccgggc gtcgaccctg cggcctcaag actgatcact cgccgaagat tcacggagtg
      721 ccgcatgggt agggtggcgg ctaccggttt ttcttgtacc cgaaccgctc ctcgaagata
      781 ggggcggtca gattgtccac cggggcataa tcatcggtca agatgacatg ggggcgcttt
      841 gccaggtgtt cctgaaggcg ctcctgggca acgacgtggg acctcatctg gaacccgcgt
      901 tcgttcctgg tgaaaccgat gaagtcgtcg aagtctattt tcttgcggct cgccacgacg
      961 acccatgtgc ttatcccctc gtaactcggg ttgatggcga cgaggttcac atgacccttt
     1021 ccgaacacct cttcgagcgt acgtatgtaa gaaggcagga agaggccttt ctcgtaattg
     1081 tcgatcaggt tagccaacag gataccgtcc ggtttgagga gcgccgccag ttgagcggcg
     1141 aattccctcg tcgtcaggtg gtaggggatc gataggtcgt taaaggcgtc gccgaagata
     1201 aaatcgtact tccctttttc ttggcagttc atcacgaacc agcgggcgtc ttcgttgaaa
     1261 gagcgtatcc gggtgtcttt cgggatgccg agatactcgt gggctatccg ggttatctcg
     1321 gggtcgatct cgacaacgtc gacctccgct gcggggtttt tcacctcgag gtaacgggga
     1381 taggtgtagc cgccgccccc gacgaagagt gtcttgaggg atttcacgtc gcgcatccgc
     1441 caggtcacga attcatcgta gatgcgcaga tattcatagt ccaggtacgc ggggttgtcg
     1501 agcacgttga tcgaatggat gaggttgtcg aggatcagcg cctccgcccg ctgtccgtct
     1561 ttggtatggt atttggagag tttgagcgtg tagtagtcgc tctccttata gagataatag
     1621 atgtccttgt cgagaggcgg tttgaaggca tacccgtaaa aggctaagac cagaggtgca
     1681 aggaaggcga tgacgacgag gacggacctc ttgcggttga acgcgccccc gaaaactaag
     1741 gcagagagga tcaggatcac cgccacggag agcaggatgt tccgcgtgcc catccacgag
     1801 atgaggaaga agcctgtggc gaatgtcccg atgatggacc cgagggtcga gaaggcgtag
     1861 attttgccga ccacatttcc cgtctccgag aggttgttga gggtgagttt caccaccaca
     1921 ggcgaaatca tgccgaggat acaagagggg agaaaaaata tgaaggtagt caggatcagg
     1981 atacggacca tcagtgtcgt cggaaacttg gcggccccga tcaggttggt caggggggag
     2041 atggccaggg cgccgaggcc ggacaggaag agaagccagc cgagggtgga tgccctcgga
     2101 tagcggtcgg cgatcacccc tcccacgtaa gcgcctatgc tgatcccggc caggacgacg
     2161 ccgatgatac tcgtccatgt atagagtgaa acccctacga aaggggccat gatacggccg
     2221 gccacgagtt cgatgaccag cgtgcagcag cttgctatg
//
LOCUS       DAOTMX010000288         2256 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_37387_length_2256_cov_3.459791, whole genome shotgun sequence.
ACCESSION   DAOTMX010000288 DAOTMX010000000
VERSION     DAOTMX010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37387_length_2256_cov_3.459791"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="P5573_07750"
     CDS             complement(<1..223)
                     /locus_tag="P5573_07750"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HRT27993.1"
                     /translation="MGFVLLAVTFAQQNPEEVTIRYFGLIPTYQVPTYLLVIVSFFAG
                     VVLTGLAVVVDRFRLTIKVAKLKKEIKDLE"
     gene            complement(252..737)
                     /locus_tag="P5573_07755"
     CDS             complement(252..737)
                     /locus_tag="P5573_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HRT27994.1"
                     /translation="MKAIMAPWRMAYIMGKKAEGCIFCQDSIRDECLVLHEGTRAFIT
                     MNRYPYTNGHLLIAPYRHVANFEDLDNEEGKEIFDLAALSVRVLKEVMRPDGFNIGIN
                     IGKAGGAGVGDHVHLHVVPRWSGDTNFMTTLAETRVVPEDLGKTHENLLPYFKKLARE
                     E"
     gene            complement(746..1228)
                     /locus_tag="P5573_07760"
     CDS             complement(746..1228)
                     /locus_tag="P5573_07760"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRT27995.1"
                     /translation="MKIGVISDTHLKNCDDRLRRIVDTYFKDVELVIHAGDLVSLEVL
                     DAFAGKEVRAVSGNMDNEDARRTLPEQMLIEVGGFKVGLTHGWGQPFGLEDKLRKKFD
                     EIDCLVFGHTHRPCNKVKDGVLYFNPGSATDRLFAKLNTVGILEIGDGVKGEIIEVSL
                     "
     gene            1400..1669
                     /locus_tag="P5573_07765"
     CDS             1400..1669
                     /locus_tag="P5573_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27996.1"
                     /translation="MIESTLEKIAERILALDEASLASLWSTYKKRMEDFDFSREWEKS
                     VIVFFIINAVRAKNQIFNDKLLKTQQGGERVKHPSTKPDLKLVKS"
     gene            1698..1970
                     /locus_tag="P5573_07770"
     CDS             1698..1970
                     /locus_tag="P5573_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HRT27997.1"
                     /translation="MRRVHVIIYGKVQGVCFRAYTQETAEALKLTGWVRNLPNGSVEA
                     VFEGPEENIAKMIERCRRGPRFAKVDSVVVKDEPYTGEFDRFSIIY"
     gene            2011..>2256
                     /locus_tag="P5573_07775"
     CDS             2011..>2256
                     /locus_tag="P5573_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT27998.1"
                     /translation="MVTTVSYHDDPRYVTHPGEGYDGVVRVSYGGYYGTGALLFDGRA
                     VLTVEHLFDGRDGTARVTFETQSGTQTLSGIKVLLHLG"
BASE COUNT          521 a          628 c          567 g          540 t
ORIGIN      
        1 tttcgaggtc tttgatctct ttcttgagtt ttgcgacctt gattgtcagt cggaaccggt
       61 ccaccaccac tgccaaccct gtcaaaacga caccggcaaa aaaagatact atgacgagaa
      121 gataagtcgg gacctggtaa gtagggatga ggccgaaata ccttatcgtt acttcttcag
      181 ggttctgctg ggcgaaggtt actgcaagca acacaaaacc catgatcaat attacgataa
      241 gcgctttcac ctcattcctc ccgagctaat tttttgaaat agggcaaaag attttcgtgg
      301 gtttttccta agtcttccgg gacgaccctc gtttcggcaa gagtggtcat gaaattcgtg
      361 tctcccgacc atctcggcac cacgtggagg tgcacatggt cgccgacccc cgcccctccg
      421 gccttgccta tgtttatccc gatgttgaag ccgtcgggtc tcatcacttc tttgagcacc
      481 ctgaccgaaa gggccgccag gtcgaatatc tccttcccct cctcgttgtc gaggtcttcg
      541 aaattagcga cgtgtcggta tggggcgatg agcagatggc cgttggtgta cggatacctg
      601 ttcatggtga taaaggccct ggtgccttca tgcaggacaa ggcattcatc cctgatcgag
      661 tcctgacaaa agatacagcc ctccgctttc ttgcccatga tgtaagccat cctccacggc
      721 gccatgattg ctttcatcct gcccctcaca gcgagacttc gatgatctcc cccttgactc
      781 cgtcccctat ctcgaggatg ccgaccgtgt tgagcttggc gaacaatcgg tcagttgccg
      841 acccggggtt gaaatagagc acaccgtcct tcaccttgtt gcatggccga tgcgtatgtc
      901 caaagacgag gcagtcgatt tcgtcgaatt tcttcctcag tttatcttcc agtccgaagg
      961 gttgtcccca gccgtgggtc agcccgacct tgaacccccc gacttcgatc aacatctgct
     1021 ccggtaatgt ccgtcgcgca tcctcgttgt ccatattgcc cgacaccgcc cttacttcct
     1081 ttcccgcgaa ggcatccaag acctcgaggc tcacgaggtc gcccgcatga atgactaatt
     1141 cgacgtcctt gaagtatgta tcgacgatcc gcctgagcct gtcatcgcaa ttctttagat
     1201 gggtgtcgga gataacgccg atcttcatct tccttttcaa cttgctgtgg aaagagattc
     1261 aacgagcgtg cgaattatat ccactcgaca gggcaaaaaa caagcatttt ttacggactt
     1321 gtagattata agaccctgct tttcatttga cttttcgtct tttccacctt aatatcttgc
     1381 caccttggaa ggagatgcaa tgatagaatc tactctcgag aaaatcgccg aaagaatact
     1441 cgccctcgac gaggcatcgc tcgcgtccct gtggtcgacc tacaagaaaa ggatggaaga
     1501 cttcgatttt tccagagagt gggagaagtc cgtcatcgtc ttctttatca tcaacgcagt
     1561 gagggccaag aaccagatat tcaacgataa actcttgaag acccagcaag gcggcgaaag
     1621 ggtgaaacac ccttctacaa agcctgacct caaactcgtc aaatcttgag acgcacggac
     1681 ttcttcaacg attcccgatg agacgtgtcc atgtaatcat ctacggcaag gtccagggcg
     1741 tatgcttccg ggcctacacc caggaaaccg ccgaggcctt aaagctgaca ggctgggtcc
     1801 ggaacttgcc gaacggtagc gtggaagcgg tcttcgaggg acctgaggaa aatatcgcca
     1861 agatgataga acggtgccgc cgcgggccga ggttcgcgaa ggtggactcc gtggtcgtaa
     1921 aggacgagcc gtacaccgga gaattcgacc gtttcagcat aatatattga tttttctggg
     1981 aaataaaact gcaaggaggt atggtccgag atggtgacaa cggtttctta ccacgacgac
     2041 cctcggtacg tcacacaccc tggggagggt tacgacggcg tcgtccgcgt cagctatggc
     2101 ggctattacg gcacaggcgc gcttctgttc gacggccgtg cggtcctgac cgtagagcac
     2161 ctcttcgacg gacgcgacgg cactgcccgc gtgacgttcg agacgcaatc gggcacccag
     2221 acgctgagcg gcataaaagt cctgttgcac ctgggg
//
LOCUS       DAOTMX010000289         2243 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_37705_length_2243_cov_3.324954, whole genome shotgun sequence.
ACCESSION   DAOTMX010000289 DAOTMX010000000
VERSION     DAOTMX010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37705_length_2243_cov_3.324954"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="P5573_07780"
     CDS             <1..642
                     /locus_tag="P5573_07780"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HRT27999.1"
                     /translation="PENSIGSDEDWEMATNALEAALKGKKMVYDVNVGDGAFYGPKID
                     FKLKDALKRKWQCATIQCDFTLPERFDLSYIGTDGEKHRPVMLHRVILGAIERFMGVL
                     IEHYAGAFPVWLSPVQAILLTVTDNQIPYGETVYQKLLDSGVRVGKDFRNEKLGYKIR
                     EAQMQKIPYMLVVGDREVESATVSPRQRDGKNIGPMTPEAFIALVQEKCAGHE"
     gene            759..1268
                     /gene="infC"
                     /locus_tag="P5573_07785"
     CDS             759..1268
                     /gene="infC"
                     /locus_tag="P5573_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012607480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HRT28000.1"
                     /translation="MAKDLRVNREIRAGSVRVIDFEGKQLGIMTLQDALRQASEAGLD
                     LVEVAPNSSPPVCRIMDYGKFRYQQSKKMQVARKHQAVLQLKEIRLRPKTEEHDLQVK
                     LRHIRRFLEENNKVKITMMFRGREIAYAEVGRKIMEGLMEGLGDIGTVDQQPKLEGRN
                     MVMIVAPKK"
     gene            1281..1481
                     /gene="rpmI"
                     /locus_tag="P5573_07790"
     CDS             1281..1481
                     /gene="rpmI"
                     /locus_tag="P5573_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005950095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HRT28001.1"
                     /translation="MLNKVKTHRGAAKRFKLTATGKVKRRKAFHSHILTSKSAKRKRN
                     LRASAVLDKRDTRGIKRLIPYL"
     gene            1564..1920
                     /gene="rplT"
                     /locus_tag="P5573_07795"
     CDS             1564..1920
                     /gene="rplT"
                     /locus_tag="P5573_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HRT28002.1"
                     /translation="MRVKRSITARKKRRKVLKLAKGYFGARSRLYTVAAEAVDRALKF
                     SFRDRRARKRDFRKLWIARINAAARLNNVTYSRLMDGLKKANISINRKILADLAVNDP
                     KGFSRIVTTALGERQA"
     gene            1917..>2243
                     /locus_tag="P5573_07800"
     CDS             1917..>2243
                     /locus_tag="P5573_07800"
                     /inference="COORDINATES: protein motif:HMM:NF014909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HRT28003.1"
                     /translation="MRDELEKLRRQALAELDGAARSEDLHAVRTKYLGRKGLLTAFLR
                     RLGEIKDEERPALGKLTNDIKQELSARVDEALRRLSAEGRHEALLAEKIDVTLPGRGL
                     KYGRLHP"
BASE COUNT          603 a          521 c          717 g          402 t
ORIGIN      
        1 ccggaaaatt ccatcgggtc ggacgaggac tgggagatgg ccacgaacgc gctcgaagcc
       61 gcgctcaagg gcaaaaaaat ggtgtacgac gtcaacgtcg gggacggcgc cttttacggg
      121 ccgaagatcg acttcaaact gaaagacgcc ctgaaacgta aatggcaatg cgcaacgatt
      181 cagtgtgact tcacgctgcc cgaacgcttc gaccttagct atatagggac ggacggggag
      241 aagcatcggc cggtgatgct gcacagggtg atcctcggcg ccattgagcg gtttatgggc
      301 gttttgatag agcattatgc cggcgccttt ccggtctggc tttctccggt gcaggccatc
      361 ctgcttaccg tgacggacaa ccagatacct tacggtgaaa ccgtttacca aaagctcctc
      421 gactccggtg tacgggtggg gaaggacttc aggaacgaaa agctgggcta caagatcagg
      481 gaggcccaga tgcagaagat accttacatg ctggtagtcg gtgacaggga ggtggagtcg
      541 gcgacggtct ctccgcggca gcgggatgga aagaacatcg ggcccatgac gccggaggca
      601 ttcatcgccc tggtgcagga gaagtgcgcc gggcacgaat aaaccggaca tcgcgcgttg
      661 gaaacgggat gtggggaggc ggggaatgat gttctccgca gagggttgtg tgagtcttca
      721 gtctgatggc gggaaacctt aacttgagga ggtgactcat cgctaaggat ttaagggtta
      781 acagggagat tagggccggt tccgtacggg tgattgattt tgaggggaaa cagctgggga
      841 tcatgactct ccaggatgcc cttcggcaag cgagcgaagc ggggctggac ctcgtagaag
      901 tggcaccgaa ttcctcaccg cctgtttgca ggatcatgga ttatggtaaa ttccgttacc
      961 agcagagtaa gaaaatgcag gtggcgagga aacatcaggc cgttttgcag ttgaaggaaa
     1021 tccgactgag gcctaaaacg gaagagcacg atttgcaggt gaagctccgc cacataagaa
     1081 gatttctgga agaaaacaat aaggtcaaga tcaccatgat gttccggggc agggaaatag
     1141 cttacgcgga ggtcggtcgg aagataatgg aagggctgat ggagggactc ggcgatatcg
     1201 ggaccgtcga tcaacagccc aaactcgagg gccgaaacat ggtcatgatc gtagcgccga
     1261 aaaaataggg gtgggacact atgcttaata aggtgaagac gcacaggggg gccgccaaga
     1321 ggttcaaatt gacggcaaca ggcaaggtta aaagacgcaa ggcctttcac agccatatac
     1381 tgactagtaa gtccgcaaaa aggaagagaa acctgagggc gtcggcggtg ctcgacaagc
     1441 gggacacgag gggaatcaag agactgatcc cttatttgta gagataaggg ccgtcaaagg
     1501 cgcctaagtt atacccacag tcaggtagta tcttagataa cgacggaaag caggagacga
     1561 ggaatgagag tcaagaggag cataacggcc agaaagaaga ggagaaaggt cctcaagctg
     1621 gcaaagggat attttggggc acgaagtcgc ctgtacactg tggccgcaga agccgtagac
     1681 cgggcgttga agttttcttt tcgcgaccgc agggccagga agcgggactt ccgcaagctc
     1741 tggattgcga ggatcaacgc agctgcacgc ctcaacaacg tcacttacag ccgtctcatg
     1801 gatgggttga agaaggccaa catcagcatc aaccgaaaaa tccttgccga ccttgcagta
     1861 aacgacccga aaggtttctc tcggattgtc acgactgccc tgggcgagcg acaggcatga
     1921 gagatgaact ggagaaactc cgccggcagg ccctggcgga attggacgga gcggcgagaa
     1981 gtgaagatct ccatgccgtc cggacaaaat atctggggag aaaagggctg ctgacggcct
     2041 ttttgagaag gttgggggaa ataaaggacg aagaaaggcc agccctcggc aaactgacaa
     2101 acgacatcaa gcaagaattg tctgccaggg tcgacgaggc actccggcgg ttgtcggcag
     2161 agggccggca tgaagcgctc ctggccgaga agatcgatgt gacgctcccc ggacgcgggc
     2221 tcaagtacgg tcgactccat ccc
//
LOCUS       DAOTMX010000290         2233 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_37947_length_2233_cov_3.848944, whole genome shotgun sequence.
ACCESSION   DAOTMX010000290 DAOTMX010000000
VERSION     DAOTMX010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37947_length_2233_cov_3.848944"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="P5573_07805"
     CDS             complement(<1..742)
                     /locus_tag="P5573_07805"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HRT28004.1"
                     /translation="MLDDNPKVCLTFDDLLLVPAESSFLPKDVDTTTWLTNNIRLGIP
                     IVSAAMDTVTESRTAITMAREGGIGIIHRNMSIQRQAMEVDKVKKSESGMIVDPITIE
                     PEQKVRDALELMSRYKISGVPVVKKRRLVGILTNRDLRFETNMDQPVADVMTKENLVT
                     VSSSITLEESKKLLHKHRIEKLLVVDDEYNLKGLITIKDIEKIEKYPNSSKDSLGRLR
                     VGAAVGIIDREQRVEALLAAGADVIVIDT"
     gene            complement(787..1275)
                     /locus_tag="P5573_07810"
     CDS             complement(787..1275)
                     /locus_tag="P5573_07810"
                     /inference="COORDINATES: protein motif:HMM:NF018624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1285 domain-containing protein"
                     /protein_id="HRT28005.1"
                     /translation="MSESKIVEKKVEEIPPCGIRVDKEGVWYFEGKEMFRRDIVNYFY
                     QNLIKDGAGRYIIVQGDERCYLEVEDTPFIVKAVFSKGSRERGDEELELLLCDDTVET
                     LDPTTLWVGPENVLYCLIRNGTFKARFSRPGYYQLAEYIDHDQEGGCYVLTLNGKKYA
                     IR"
     gene            complement(1282..1725)
                     /gene="dtd"
                     /locus_tag="P5573_07815"
     CDS             complement(1282..1725)
                     /gene="dtd"
                     /locus_tag="P5573_07815"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HRT28006.1"
                     /translation="MKAVVQRVESTRVVVDGREVSRIGKGLLVFLGVAKDDDRKDADY
                     LLDKIINLRIFEDPEGKMNASLLDLSAELMVVSQFTLLADCRKGRRPSFGEAADPAIA
                     KPLYEYFVEQSQKKVRAAGGIFQAMMRVELVNDGPVTILLDSKKA"
     gene            complement(1779..1855)
                     /locus_tag="P5573_07820"
     tRNA            complement(1779..1855)
                     /locus_tag="P5573_07820"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1819..1821),aa:Pro,seq:tgg)
     gene            complement(1868..>2233)
                     /locus_tag="P5573_07825"
     CDS             complement(1868..>2233)
                     /locus_tag="P5573_07825"
                     /inference="COORDINATES: protein motif:HMM:NF013854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HRT28007.1"
                     /translation="ALDGRPGVRSSRYAGPEANDDENIEKLLLELRDVPQEKRGAAFR
                     CTLVMYRPDGRYDTFEGSLRGLITTEPRGKQGFGYDPVFFVPEIDKTVAEMPLELKNS
                     ISHRGAALRALKNHLKIGG"
BASE COUNT          419 a          689 c          567 g          558 t
ORIGIN      
        1 aggtatcgat cacgatgacg tcggcccctg ctgcgaggag cgcctcgacc cgctgttccc
       61 ggtcgatgat ccccaccgcc gcccctaccc gcagccttcc caacgaatcc ttgctggaat
      121 tcgggtattt ctcgatcttc tcgatatcct ttatggtgat gagccccttc aagttgtact
      181 cgtcgtccac taccaacagt ttctcgatgc ggtgtttgtg gagaagcttc ttcgattctt
      241 ccagggtgat cgacgaggaa acggttacca ggttctcctt cgtcatcacg tcggccacgg
      301 gctggtccat gttcgtctcg aaccgcaggt cccggttcgt caggatcccg acgagacggc
      361 gttttttgac caccggaaca ccggatatct tgtagcggct catgagctcg agtgcgtccc
      421 tcaccttctg ctcgggctct atcgttatcg ggtctacgat catcccgctc tccgattttt
      481 ttaccttgtc gacctccatc gcctgtctct ggatgctcat gttacgatgg atgatcccta
      541 tgccgccttc tcgggccatc gtgatggccg tcctcgactc cgtcaccgta tccatcgccg
      601 cgctcactat cgggatgccc aaacggatgt tgttcgtcaa ccacgtggtc gtgtcgacgt
      661 ctttcgggag gaaagaggat tcggcgggaa cgagcagaag gtcgtcaaag gtaaggcata
      721 ccttagggtt atcatccaac atacgcacct ccgcgattga gtctctcttc cgtgggtcta
      781 tggagtttat ctgattgcat actttttgcc gttcagggtg aggacgtagc agcccccttc
      841 ctggtcatgg tcgatgtact cggccaactg atagtagccg ggccgtgaga atctcgcctt
      901 gaaagtccca tttcttatga ggcagtagag tacattttcg ggaccgaccc agagggtcgt
      961 ggggtcaagg gtctcgacgg tgtcatcgca caggaggagc tccaattcct cgtctcccct
     1021 ctctcgggaa cctttgctga aaacggcctt cacgatgaac ggggtgtcct cgacctccag
     1081 gtagcaccgt tcgtccccct ggacgatgat gtagcgaccg gccccatctt ttataaggtt
     1141 ctgataaaag tagttgacga tgtccctgcg gaacatctcc tttccttcga agtaccaaac
     1201 cccctccttg tcgaccctga ttccacaggg aggtatctcc tcaactttct tctctacaat
     1261 cttagattca ctcatgttct gtcatgcctt tttgctgtcc aggagtattg tcaccgggcc
     1321 gtcgttgacg agctccaccc tcatcatggc ctggaaaatc cctccggccg cccggacctt
     1381 cttctgtgac tgttccacaa aatactcgta gaggggctta gctatggccg ggtctgccgc
     1441 ctcaccgaaa gagggacggc ggcccttacg gcaatccgcc aggagcgtga actgcgagac
     1501 caccatcaat tccgccgaga ggtcgagcag ggaggcgttc atcttgcctt ccggatcctc
     1561 gaaaatcctc aggtttatga tcttgtccag gaggtagtcc gcgtcttttc tgtcgtcgtc
     1621 tttcgccacc cccaaaaaaa cgagcagccc ttttcctatc cgggagacct ctctcccgtc
     1681 gacgaccacc ctagttgatt ctacgcgctg cacaaccgcc ttcatccgcc catcttaaca
     1741 cggtgaattc atcacattcg acccgtttgc cccggaaatg gtcggggcga gaggatttga
     1801 acctccgaca tcctgctccc aaagcaggcg cgctaccagg ctgcgctacg ccccgtattc
     1861 cggtatgtta tccgccaatc ttcaagtgat tcttaagcgc tcgaagagcc gcgccccgat
     1921 gacttatact gtttttgagc tccagcggca tctcggcgac cgtcttgtct atttcgggga
     1981 caaagaatac ggggtcgtac ccgaacccct gtttgccccg cggctccgtt gtgatcaacc
     2041 cccgcaacga accctcgaag gtatcgtatc tgccgtcagg cctgtacatg acgagggtac
     2101 agcggaatgc ggccccccgt ttttcctgag gcacatcccg caattcgagg aggagtttct
     2161 ctatgttctc atcgtcgttt gcctccgggc ctgcgtaacg ggaagaacgg acgccggggc
     2221 ggccgtccag ggc
//
LOCUS       DAOTMX010000291         2211 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_38435_length_2211_cov_3.833024, whole genome shotgun sequence.
ACCESSION   DAOTMX010000291 DAOTMX010000000
VERSION     DAOTMX010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38435_length_2211_cov_3.833024"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..1253)
                     /locus_tag="P5573_07830"
     CDS             complement(57..1253)
                     /locus_tag="P5573_07830"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRT28008.1"
                     /translation="MPYYIGDVIKEYKRLVIRTPEDFKKTGIDVKIKTRVEGIEPAKG
                     TVHLSDGSALPYDILVMATGAEAVKLGLPGEDLEGVFTLRNLTDALRIKSFLNEKKCR
                     KAVIVGAGYIGMEMCEAFRNNGLEVQVIDILPRPVLRWDAEFAKLILDELGKNQVTFM
                     PETKPLSIERGKDSALRLVTDKGNLEADIILIGVGSRPNTKLAESLGVEMGRSAIKVD
                     FFQRTSREEVYAVGDCCEVFHRVIGKWTYNPLGDVANKQGRTAGQNIGGASTKFPGVV
                     GAQSFKVFNLEAAATGITEEEALNHGFQPVSAIIQGLPVGRPMARGEKLALKLVADKA
                     TGKLLGAQSVAEKGSVTRINCLSVALWNGMTLEEIGHLDLAYAPPFGGAWDAIHIAAQ
                     MLMRKL"
     gene            complement(1257..1388)
                     /locus_tag="P5573_07835"
     CDS             complement(1257..1388)
                     /locus_tag="P5573_07835"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRT28009.1"
                     /translation="MSKNVVIIGGGAAGPSTAAEAQRRDPSLKITMVERGEFVSFAA"
     gene            complement(1405..1944)
                     /locus_tag="P5573_07840"
     CDS             complement(1405..1944)
                     /locus_tag="P5573_07840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HRT28010.1"
                     /translation="MDKEAKIEEIKQRARKNFSLGYNCAECVTEAVLSLVDTGLPGEA
                     KKLATGFGGGGGLFGGTCGAVCGAIMAVGAVHGRSKLPEGEGKEAAMKSKEQLYGKPG
                     LYRLFNIIPNRIEEKYGSTLCGDLTKQWQSSWLCRDHALFCRDLITEAAGIAAELIFA
                     DKNELASRPFGKNVENLKD"
BASE COUNT          409 a          687 c          578 g          537 t
ORIGIN      
        1 atgaaggaaa gaaaaaagcc cggggcagcc ttgaacgacg gccccgggcc tttccgtcac
       61 agctttcgca tcagcatctg ggccgcgatg tggattgcgt cccaggcccc accgaagggc
      121 ggggcgtaag cgaggtccag atggccgatc tcttcgaggg tcatgccgtt ccagagggct
      181 acggagagac agttgatcct cgtgacggag cctttttcgg cgaccgattg ggcgccgagc
      241 agtttccccg tggccttgtc ggcgacgagc ttcaacgcca gtttttcgcc tctcgccatt
      301 ggacgtccga caggcagtcc ctggattata gcgctcacgg gctggaagcc gtgattcagg
      361 gcctcttctt ccgtgatgcc ggtggcggcg gcttcaaggt tgaagacctt gaaagactga
      421 gcgcccacga cgccgggaaa ttttgtcgac gctccgccga tattttggcc ggccgtacgc
      481 ccctgcttgt tcgccacatc ccccagcggg ttgtatgtcc atttgccgat gacgcggtgg
      541 aagacctcgc agcaatcgcc cacggcgtac acctcttccc gggaggtacg ctggaagaag
      601 tctaccttga tggcgctcct acccatttca acgcccagtg attccgccag cttcgtgttc
      661 ggtctcgagc cgaccccgat caggattatg tctgcctcga ggtttccctt gtcggtcacc
      721 agccggagcg cggagtcctt ccccctttcg atggaaaggg gctttgtctc gggcatgaaa
      781 gtcacctggt tcttgccgag ttcatctaag atgagcttgg cgaattcggc atcccacctc
      841 agcaccggcc gggggaggat gtcgatcacc tgcacttcca acccgttgtt gcggaaggcc
      901 tcgcacattt ccatgccgat gtagccggcc cctacgatga cggccttccg acattttttt
      961 tcgttcaaga aggactttat cctcaaggcg tccgtcaggt tacgcaaggt gaagacacct
     1021 tccaggtctt cgcccggaag tccgagtttc acggcctccg ccccggtcgc catgacaagg
     1081 atatcgtagg gcagggccga gccgtcggag agatgtacgg tgcctttcgc cggttcgatt
     1141 ccctccacgc gagtctttat cttgacgtca atgcctgtct ttttgaaatc ctcgggagtc
     1201 ctgatgacga gtcgcttgta ctctttgatt acatcaccga tgtaataggg catcggtcag
     1261 gcggcaaagg aaacaaattc cccccgctcc accatcgtga tcttaagaga agggtcccgc
     1321 ctctgtgcct ccgccgccgt ggaaggaccg gcagcgccgc cgcctatgat tactacgttt
     1381 ttgctcatcg ttttttccct tttctcaatc cttcaggttc tcgacgttct ttccgaaagg
     1441 cctcgaggcc agttcgttct tgtcggcgaa gatcaactcc gccgcgattc ctgctgcttc
     1501 cgttatcaag tcacggcaga acagtgcatg gtcacgacag agccaactgg actgccactg
     1561 ctttgtcaag tcgccgcaga gagtgctccc gtacttctcc tcaatcctgt tcggaatgat
     1621 gttgaacaag cggtacaggc ccggtttgcc gtaaagctgc tccttggact tcatcgccgc
     1681 ttccttacct tcaccttcgg ggagtttgct ccgcccgtgt accgccccca cggccattat
     1741 cgccccgcag accgccccgc aggtcccccc aaaaaggccg ccaccgcccc caaatcccgt
     1801 ggccagcttc ttggcttccc ccggtaaccc cgtatctacg agactgagta ccgcctcggt
     1861 cacgcactcc gcacagttgt accccagact gaaatttttc ctggcccgtt gcttgatttc
     1921 ttctatctta gcttccttat ccatcgtata atctcccccc tttctcgaat tgggggagat
     1981 tatacggcag cgtcgagtat tgtcaagcat ccgttgcttt cattttcatt tgactcttgc
     2041 aattttcgcc gtatctggta ccaattgccg actttagacg aaccgacgaa ccacccaaga
     2101 catccttctc tggaggtttt tgataatgcg aaattttctt gaaccggggt ccgtggcctt
     2161 catcggtgcc tcccggaaga cgggaacagg tgcgtacaac ggcgtggaga t
//
LOCUS       DAOTMX010000292         2208 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_38506_length_2208_cov_3.454714, whole genome shotgun sequence.
ACCESSION   DAOTMX010000292 DAOTMX010000000
VERSION     DAOTMX010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38506_length_2208_cov_3.454714"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="P5573_07845"
     CDS             <1..540
                     /locus_tag="P5573_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT28011.1"
                     /translation="HELQNILEQDLDGYPEVELPTTPDWLADHVRNGARTAHQYYETC
                     IEHYDLAKGVRNPPAGLDQELKDILAELIGLAAVGFARLLERGFDESQVQPPEVELVL
                     DTIIAQARRPVNWVWGCCVATRRITTAWSTSATLPGSRRRSKRPGCRPGYNRPSRCAR
                     SPNLSSSGGLFDTWKEEGS"
     gene            471..869
                     /locus_tag="P5573_07850"
     CDS             471..869
                     /locus_tag="P5573_07850"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT28012.1"
                     /translation="MRQVAEPFIIRRAVRHLERGRIVIFAAGTGNPFFSTDTAAALRA
                     MEIRAEVLLKATKVDGVYGRDPNIYPDAEYYKSISFTDVLSKNLKVMDSASISLCRDN
                     DLPIVVFNLEKKGNIKGVVCGESIGTVVRG"
     gene            874..1431
                     /gene="frr"
                     /locus_tag="P5573_07855"
     CDS             874..1431
                     /gene="frr"
                     /locus_tag="P5573_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HRT28013.1"
                     /translation="MNDRIGKDLKAQMEGILNSLEKSLGKIRTGRASLSLLDGIKVDY
                     YGSPTPLNQVASFSIPESRQILISPWDPGALNSIEKALLKSELGLTPNNDGKLIRINI
                     PPLTEERRKELVKVVKKMGEDAKIKLRNARRDTNEQLKALKKENSISEDELFKQQGEV
                     QKLTDEYIKRIDEIIVAKEKEIMEI"
     gene            1546..>2208
                     /locus_tag="P5573_07860"
     CDS             1546..>2208
                     /locus_tag="P5573_07860"
                     /inference="COORDINATES: protein motif:HMM:NF011405.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HRT28014.1"
                     /translation="MVRIDEKRVPRHIAIIMDGNGRWARQNALGRISGHREGAESVRV
                     VVEACRKIGVPYLTLFAFSVENWLRPNPEVRALMRLLVRYLRSELKTMLKNDIRLRTI
                     GNVADLPENVRTVLKGAVDATKHCKGMVLNLALSYGGRDDIIGAVKKIMKDSAAGKIK
                     ASEITRESFEKYLSTRDIPDPDLLIRTSGEFRISNFLLWQSAYTELYFTDVLWPDFRE
                     PQL"
BASE COUNT          566 a          533 c          678 g          431 t
ORIGIN      
        1 cacgagctgc agaacattct cgaacaggat ctggacggct acccggaggt cgaactgccg
       61 acgacgccgg actggctggc cgaccacgtg cggaacggcg cccggacggc gcatcagtac
      121 tacgagacct gtatcgagca ttacgacctg gccaagggag tccgcaatcc gccggcgggc
      181 ctcgaccagg agctgaagga cattctcgcg gagctgatcg ggctggcggc ggtcgggttt
      241 gcgcggctgc tggaacgggg atttgacgag tcgcaggtgc agccgcccga ggtggagctg
      301 gtgctcgaca cgatcatcgc gcaggcgcgg cggccggtga actgggtttg gggctgctgc
      361 gtggcgacac gtcgcattac tacggcgtgg tcaacgtcgg cgacgctgcc gggctcaaga
      421 aggcgctcga aaagaccggg ctgccgacca gggtacaatc ggccatcgag atgcgccagg
      481 tcgccgaacc tttcatcatc aggcgggctg ttcgacactt ggaaagagga aggatcgtga
      541 tcttcgcggc gggaacggga aacccctttt tttccacgga tacggctgct gccttgcggg
      601 cgatggaaat ccgagccgag gtcttgttga aggcgaccaa ggtagatggg gtgtatggtc
      661 gggaccccaa tatttatccc gacgctgagt attataaaag tatcagtttt accgatgtat
      721 tatcgaaaaa ccttaaagta atggattcgg catcgatttc tttatgtcga gacaatgatc
      781 tgccgattgt cgtgttcaac cttgagaaga aaggtaacat caaaggggtc gtctgcggtg
      841 agtcgatcgg tacggttgtg agaggctgag aagatgaacg acaggatcgg gaaagatttg
      901 aaggcccaga tggaggggat tctcaactcg ttggagaaat cattgggaaa gatccgcacg
      961 ggacgggcgt cattgtctct cctggacggt atcaaagtcg actattacgg gtcgccgacg
     1021 ccgctcaacc aggtggcttc tttttccatc ccggaaagtc ggcaaatctt gatctctccg
     1081 tgggatcccg gtgcactgaa cagcatcgaa aaggcgctcc tgaagtctga gcttggcctg
     1141 acgccgaaca acgacgggaa gctcatcagg ataaacatac cgcccttgac agaggaaaga
     1201 agaaaagaac tcgtcaaggt ggtaaaaaaa atgggtgagg acgccaagat caagcttagg
     1261 aacgcaagaa gagacacgaa cgaacagctt aaggcactca agaaggaaaa ctcgatctcc
     1321 gaagacgaat tgttcaagca gcaaggtgag gtccagaagc tgaccgatga gtacatcaag
     1381 aggatcgatg aaatcatcgt cgcaaaggag aaggagatta tggagatata ggggtcgaag
     1441 actgccgtaa tctatcgaat caggagccca gggctccttt tttgtttggt ataccgccgc
     1501 gatgttgaaa atgttcatgg cctatggtac ggagaaataa aagaaatggt cagaatagac
     1561 gaaaaaaggg taccgagaca tatcgccatc atcatggacg gcaacggccg gtgggccagg
     1621 cagaatgccc tggggaggat ttcgggccat cgtgaaggtg cagaatcagt gcgcgtcgtc
     1681 gtcgaggcgt gcagaaaaat cggcgttcct tacctgaccc ttttcgcttt ttccgtcgaa
     1741 aactggctgc ggccgaaccc cgaagtcagg gccctcatga ggttgctcgt gaggtactta
     1801 cggagcgaac tcaagacgat gctgaaaaac gacatccgtt tgaggacgat cggaaacgtt
     1861 gcggacctgc cggaaaacgt tcgcaccgtt ctcaaggggg ctgtagatgc gacaaaacac
     1921 tgcaagggga tggtccttaa cctggcgctc agttacgggg gcagggacga cataatcggg
     1981 gcggtaaaaa aaatcatgaa agattcagcg gccgggaaga tcaaggcgtc ggagatcacg
     2041 cgggaatcct tcgagaaata cttgagtacg agggatatac cggaccccga cctcctgatc
     2101 cgtacgagcg gcgagttcag gatcagcaat tttctgctct ggcagtcggc ctacacagag
     2161 ctctatttta cagacgtact atggcctgac tttcgcgaac cccagctc
//
LOCUS       DAOTMX010000293         2197 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_38755_length_2197_cov_4.293651, whole genome shotgun sequence.
ACCESSION   DAOTMX010000293 DAOTMX010000000
VERSION     DAOTMX010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38755_length_2197_cov_4.293651"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /gene="rpsO"
                     /locus_tag="P5573_07865"
     CDS             complement(<1..188)
                     /gene="rpsO"
                     /locus_tag="P5573_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HRT28015.1"
                     /translation="MLELEQKQKIIGSFQAHEKDTGSPEVQIALLSARIKYLTEHFKA
                     HKKDHHSRRGLLKIVGQR"
     gene            complement(230..1180)
                     /gene="truB"
                     /locus_tag="P5573_07870"
     CDS             complement(230..1180)
                     /gene="truB"
                     /locus_tag="P5573_07870"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HRT28016.1"
                     /translation="MDGVIVVDKPAGRSSFDVVKVVRALPGVEKAGHTGTLDPLATGV
                     LPVCINEATKLVQFLCMDSKDYRCTMLLGVETDTLDIEGDVLAREEVNIGVRDVEDVL
                     KSCTGKIEQVPPRYSALKHKGKTLYSLARKGIYVQPAPRRVEIHRIELLDVRLPYVTF
                     LVSCSKGTYIRSLCADVGERLGTKACVAGLRRLRSGSFHEEGALSVEELRNRRPGDVA
                     CRLISMADALAGMPAVYVDERCAARLRRGHQPTVRDLNGNNMPSLAAGDMVKFVLKLD
                     ELVAVGAVLHDSERRALLAPDERAVRIKRIFNGSEVHRSA"
     gene            complement(1185..2132)
                     /locus_tag="P5573_07875"
     CDS             complement(1185..2132)
                     /locus_tag="P5573_07875"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HRT28017.1"
                     /translation="MKRVADVIRAKTTFLVTSHVRLDGDAVGSELALFHLLRNMGKKV
                     LVYNQDETPRNLRFLPGAETIVHKLDGISEYDVLFVLDCSEVERVGEAASLIRTAPCL
                     VSIDHHISNKGDFSLSVIDPSASSVGELIFRLIDEMGADVTKEIAVNLYTAIVTDTGS
                     FRYSNAGKNTFAIAGKLVEAGAKPHKIAEKVYESNPPEKMKLLAKVLETLECHWDGKI
                     STLFVSQAMIREAGALPEYTENFVDFARAIEGVEVAAFFIEMDGGRYRVSLRSKGRIS
                     VEKVARTYGGGGHFNAAACIISGSLVDVRDRIVQSIMAG"
BASE COUNT          453 a          690 c          542 g          512 t
ORIGIN      
        1 ctcctctgtc ccacgatctt caacagaccc ctccgggaat ggtgatcctt tttgtgcgcc
       61 ttgaagtgtt ccgtcaggta ttttatcctt gcactgagaa gggcgatctg cacttccggc
      121 gacccggtat ctttctcatg agcctggaaa ctgccgatga tcttctgttt ctgttctaac
      181 tccaacacct gcttttcctc cctcgatttg attttacaac cgtaaatctt taggccgaac
      241 ggtgaacttc cgaaccgttg aatatccgct tgatccgaac ggccctctca tccggtgcca
      301 gaagagcccg cctttccgag tcatggagca ctgcaccgac agcaacgagt tcgtccagtt
      361 tgagcacgaa ctttaccata tctcccgcgg caagagaagg catattattc ccgttcaagt
      421 cccgaaccgt gggttggtgt cctctccgta atcgcgcggc gcatctttca tccacgtaga
      481 ccgccggcat acccgcaagg gcatcggcca tggaaatcag gcgacaagcg acgtcaccgg
      541 gccgccggtt tctcaattct tcgacggaca gggccccctc ctcgtggaac gaaccgcttc
      601 tcaacctccg caatcccgca acgcatgcct tggttcccaa ccgctcgccg acatcggcac
      661 acagcgaacg gatgtaggta cccttcgagc aagaaaccag gaaggtgacg tagggtagcc
      721 ggacgtccag aagttcgatt cggtggatct ccacccggcg cggggccggt tgaacataga
      781 tgcccttcct cgccaggctg tagagagttt tccctttatg cttgagagcc gaataccggg
      841 gcggcacctg ctcgatttta cccgtgcagc tcttcaggac atcttcgacg tctctcaccc
      901 cgatgttcac ctcttccctc gccaacacat ccccctctat atccagggtg tccgtctcca
      961 cccccaacag catggtgcat cgataatctt tgctgtccat gcatagaaac tgcacgagct
     1021 tggtggcctc attgatgcaa acagggagca cacccgttgc gagagggtcc agggttcccg
     1081 tatggccggc cttttccacc cccggaagcg cccgcacgac cttcactaca tcgaaggaac
     1141 ttctgcctgc aggcttatcg acaacgatca cgccgtccat acgatcaacc agccatgatg
     1201 gactgaacta tacgatccct gacgtccacc agagagccgg atattatgca ggcggccgcg
     1261 ttgaaatggc cgcctccgcc gtacgtccgc gccaccttct cgacgcttat cctgcccttg
     1321 gaacgcagac tcaccctgta gcggcccccg tccatctcaa taaaaaaagc cgccacctca
     1381 acaccttcga tagcccgggc gaagtcgacg aaattctccg tgtattccgg gagtgcgccc
     1441 gcttcacgga tcatggcctg agaaacaaag agggtgctga tcttaccatc ccagtggcac
     1501 tccagcgttt cgagcacttt tgccagaagt ttcatcttct ccggcggatt gctttcgtaa
     1561 actttctcgg ctattttatg tggtttcgcg cctgcctcga caagcttccc ggctatggcg
     1621 aaggtgttct tgcccgcgtt cgaatagcgg aaagagccgg tgtccgtcac gatggccgtg
     1681 taaagattca cggcgatctc cttcgtgacg tcggccccca tttcatcgat caaccggaat
     1741 ataagctccc cgacggaact tgcggacggg tcgatgacgg acaggctgaa atcgccttta
     1801 ttggagatgt gatggtctat actcaccagg cagggtgccg tccggatgag ggaggctgcc
     1861 tctcccaccc tttcgacctc gctgcagtca agaacgaaca ggacgtcgta ttcggagatt
     1921 ccgtcgagct tgtgaactat cgtctcggcc cccggaagaa accgcaggtt cctcggtgtc
     1981 tcatcctggt tgtagacgag caccttttta cccatgttac ggagtaggtg gaagagcgcc
     2041 agttcggacc caacggcgtc cccgtcgagc ctgacatgcg aggtcacgag aaaggtcgtt
     2101 tttgcccgga tgacatcagc aacccttttc atgtctcgtg acctttctct tttttcaccg
     2161 tctcgagaat tcgatcgatc cggcttccgt actcaaa
//
LOCUS       DAOTMX010000294         2174 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_39317_length_2174_cov_2.929212, whole genome shotgun sequence.
ACCESSION   DAOTMX010000294 DAOTMX010000000
VERSION     DAOTMX010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39317_length_2174_cov_2.929212"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="P5573_07880"
     CDS             <1..816
                     /locus_tag="P5573_07880"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HRT28018.1"
                     /translation="LNAIITLFVIGLVITSAMMLKRIPGALILGAIIVSFMALVVSQA
                     GWVAKPIVTLPERIFSWPSLDVFFKLDITGALTTGMIMPVFSLFFVDLFDSIASFVGI
                     SQVSGLTEEDGTPLEIGKALLVDAASTTISGLFGTSSGTVYVESAAGIEEGGRTGLTA
                     IVTGLLFLPFMFLSPLLAFVPDVATAPVLVLVGVFMAQPLILIDWKDMEEAIPAFLAL
                     ILIPLTFSITQGVVWSFLIYTLIKLALGKAKDVHWMLYVIDGFAILTLCQPLL"
     gene            complement(887..>2174)
                     /locus_tag="P5573_07885"
     CDS             complement(887..>2174)
                     /locus_tag="P5573_07885"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRT28019.1"
                     /translation="TVSHEVVKKDGVRAHVETLFSLLKNSDGKVIGFRGINRDITERK
                     RLEAQLRQAQKMEAIGTLAGGIAHDFNNLLSGIIGHAALMKMEPGVTKSQERRLKSIE
                     ELVQSAANLTSQLLGFARLGRYEVGTLDINEILKKTSTMFGRTRKEIMIHRELAQGLM
                     LVEADRTQMEQVFMNLFVNAWQAMPGGGDLHIKTCRTVVGDDETEETFMKPGDYVKVS
                     VTDSGEGMDEATKNRIFEPFFTTKEMGRGTGLGLAMVYGIVKGHKGYINVYSEKGHGT
                     TFNVYLPASEKEPVREEPKPRQEWLTGSETILVVDDEDQILEVMEEVLKTMGYTVFAA
                     RSGKEAVRMFRKEKGKVDLVILDMIMPGIGGGETFNRLKGVDPGVKVILSSGYSIDGA
                     AREIMGRGCKAFIQKPFSVAELSRKVREVLDGRDGA"
BASE COUNT          411 a          649 c          533 g          581 t
ORIGIN      
        1 ctgaatgcca tcataaccct gttcgttatc ggccttgtga ttacctcggc tatgatgctc
       61 aaaaggatac ccggcgccct gatattgggg gcgatcatcg tctcgttcat ggccttggtt
      121 gtatcccaag cgggatgggt tgccaaacct atcgtgaccc tgcctgagcg catcttctcc
      181 tggcccagtc tggatgtgtt tttcaagctc gacatcaccg gtgcgctgac cacaggcatg
      241 atcatgccgg tcttctcttt gttttttgtg gacttattcg actccattgc ctcgtttgtg
      301 ggtatatccc aggtatccgg cctaacggag gaggacggca cgcccttgga aataggcaag
      361 gccctcctgg tggatgcagc ttctaccacc atttcgggat tgttcgggac atcctcggga
      421 acagtgtatg tagaatcggc ggccgggatt gaagaaggtg ggcgcaccgg tctcaccgcg
      481 atagtgaccg gtctactgtt cttaccattt atgttcctgt cacccttatt ggccttcgtg
      541 cccgatgtgg ccacggcacc ggtgctcgtt ctggtgggcg tcttcatggc ccagcctctg
      601 attctgatcg attggaaaga catggaggaa gccatccccg ccttcctggc attgatcctc
      661 atcccgctga cattcagcat cacccaaggg gtggtctgga gctttttgat ctacaccctg
      721 ataaagctgg cgctcggtaa agccaaggac gtgcattgga tgctttacgt cattgatgga
      781 ttcgccatac tcaccctgtg tcagccgctg ttgtaaacat cttggtcgat gcgtttgggc
      841 tgaggtgtag atggtggtgg gatcgagcga ggaattctgc ttttactcag gcaccgtcac
      901 ggccatccaa tacctcccga accttccgag aaagttctgc cacgctaaag ggtttttgga
      961 tgaaggcctt acaacccctc cccatgatct ccctcgccgc accgtcgata ctgtatccac
     1021 tcgataaaat caccttgaca ccgggatcga cgcctttcag tcgattgaag gtttcgccac
     1081 cacctattcc cggcatgatc atgtcgagga tgacgaggtc gactttaccc ttctcttttc
     1141 taaacatcct gacggcctcc ttgccacttc gggccgcaaa gaccgtgtaa cccatggtct
     1201 tcaaaacttc ttccatgacc tcaagaatct gatcctcgtc gtcgacgaca aggatcgtct
     1261 cactgccagt gagccactcc tgccgcggtt ttggctcttc tcttaccggc tctttttccg
     1321 atgcggggag atagacattg aaggttgtcc catggccttt ctcactgtaa acattgatgt
     1381 accccttgtg gcctttcacg atcccgtaga ccatcgccaa cccaagcccc gtcccccgtc
     1441 ccatttcctt ggtggtgaag aagggctcga aaatccggtt cttcgtcgct tcatccatcc
     1501 cctccccgct gtcggtcacg gataccttaa cgtagtcccc cggtttcatg aaggtttctt
     1561 ccgtctcatc gtcgccgacc accgtcctgc atgtctttat gtggagatcg ccccctccgg
     1621 gcatcgcctg ccaggcattg acaaagagat tcatgaagac ctgctccatc tgcgtccggt
     1681 cagcctcgac gagcataagg ccttgggcta actctctgtg aatcatgatc tccttgcggg
     1741 ttctgccgaa catcgtagaa gtttttttca gtatctcatt gatgtccaag gtcccgacct
     1801 cataacgccc caggcgggca aaacccagga gctggctcgt gaggttggcg gcgctctgaa
     1861 ccagttcttc gatcgatttc agccgtcttt cttgactttt tgtcacgccc ggctccatct
     1921 tcatgagggc cgcgtggccg ataatccccg aaaggagatt gttgaaatca tgggcgattc
     1981 ctcccgccaa cgtcccgatc gcctccatct tctgcgcctg tcggagctgg gcttcgagcc
     2041 gcttgcgctc agtgatgtct cggttgattc cgcgaaaccc gatgactttg ccgtcgctgt
     2101 ttttcagcag agaaaacaaa gtctcaacat gcgccctcac tccatccttt ttcacgacct
     2161 cgtgactgac ggtt
//
LOCUS       DAOTMX010000295         2170 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_39422_length_2170_cov_3.475650, whole genome shotgun sequence.
ACCESSION   DAOTMX010000295 DAOTMX010000000
VERSION     DAOTMX010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39422_length_2170_cov_3.475650"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="P5573_07890"
     CDS             complement(<1..217)
                     /locus_tag="P5573_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT28020.1"
                     /translation="MSLVRYTVQNPALRWRYLKQVVFFGVVRLCVTVIAVSLGGILLY
                     IFINGIGAVSLDFLTEAPRDSMTAGGIM"
     gene            complement(214..1101)
                     /gene="pstC"
                     /locus_tag="P5573_07895"
     CDS             complement(214..1101)
                     /gene="pstC"
                     /locus_tag="P5573_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HRT28021.1"
                     /translation="MSRKLKEAIIENLFLFFTGGSILILGLIVFFLFREALPIFRVAT
                     ITDFIFGTEWYPTYDPPEFGIWPLIVASFIVTFFSTLIAVPVGVLSAVYISEIAHPLM
                     KEFLKPVIELLAGIPSVVLGFFGMVVVAPWMQETFDLPTGLNIVNASVMLAIMAIPTI
                     SSISEDALYSVPREFKEASYALGATKCETILRVTIPAALSGISTAVILGMARAIGETM
                     VVLMVAGGAAAIPESLFDAVRPMPASIAAEMGEAPFGSNHYHALFAIGVVLFFLTLLF
                     NLVADYLSHRFREVGSATL"
     gene            complement(1127..1945)
                     /locus_tag="P5573_07900"
     CDS             complement(1127..1945)
                     /locus_tag="P5573_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HRT28022.1"
                     /translation="MSRTLKCIAATLVALFFTPVFAVAAETVVIKGSTTVLPIAQAAA
                     EAFMKKRPDINISLSAGGSGDGIKALIDKSTDIANSSRDMKPEEVKLAGERGVEPVAH
                     RVAIDAIVPIVHPTNPVTDLTIEQLSLIYQGKITNWKEVGGADKKIVVISRDTSSGTY
                     ETWEQKVLHKAKVTPRAQLQASNGAIVQAVSKNKYAIGYIGIGYLNKSVKALRVAGVQ
                     ASAQTALSGQYPIARPLFMFTNGQPKGATAEFIKFLLSPEGQKIVKKEGFVPLK"
BASE COUNT          523 a          602 c          592 g          453 t
ORIGIN      
        1 gcatgatgcc ccccgccgtc atcgaatctc gtggggcctc cgtcaggaaa tccaagctga
       61 ccgctccgat gccgttgata aagatgtata acaaaatgcc gccgagagag actgcgatga
      121 cggtcacaca cagcctcacg acaccgaaaa aaaccacctg cttgagatag cgccatctca
      181 gcgccggatt ttgaaccgtg tatctcacga gactcacagt gtcgccgaac ccacttccct
      241 gaacctgtga gaaaggtagt cggcaaccag gttgaagagc aacgtcagga aaaataggac
      301 gacgccgatg gcgaagaggg cgtggtaatg attgctgccg aagggcgcct cgcccatctc
      361 cgccgcgatg cttgccggca tgggcctgac ggcgtcaaac aggctctcgg gtatcgccgc
      421 cgcccctccc gccaccatca gaacgaccat cgtctcgcct atcgccctgg ccatcccgag
      481 tatgaccgcc gtcgagatac ccgaaagcgc cgccggtatc gtcacccgca aaatcgtctc
      541 gcattttgtc gcacctaacg cataagacgc ctccttgaat tcccgcggca ccgaataaag
      601 ggcatcttcg gaaatactcg agatggtggg tatggccatg atagccagca tcacggaggc
      661 gttgactatg ttcagtcccg tcggcaggtc aaaggtctcc tgcatccacg gggcaacgac
      721 caccatgccg aagaaaccga gcacgactga cggtatcccg gcaagtagtt cgatgacggg
      781 cttcaaaaac tccttcatca gcggatgcgc gatctcggaa atgtacacgg cagacagaac
      841 gccgacgggc acggcgatca gggtagagaa aaatgtcacg ataaacgacg caacgataag
      901 gggccatatc ccgaactcgg gtggatcgta ggtgggatac cactctgtcc cgaagataaa
      961 atcagtgatg gtggccaccc tgaagatggg tagggcttca cggaacaaga agaaaacgat
     1021 gagtcccaga atcaggatgg aaccaccagt gaagaaaaga aaaaggttct caatgatagc
     1081 ctctttgagc ttcctggaca taaatcgttt ccgccgcatt cttcttttac ttcagaggga
     1141 caaatccctc cttttttacg atcttctgcc cctccgggct gagcaaaaac ttgatgaact
     1201 cggcggtcgc cccctttggc tgcccgttcg taaacatgaa caaaggccgc gcaatcggat
     1261 actgcccgga aagggcagtc tgcgcggacg cctgaacacc cgcaaccctc aaagccttta
     1321 cggacttgtt aaggtagccg atcccgatgt acccgatggc gtatttattc ttcgagacgg
     1381 cctggacaat ggccccgttg gaggcttgta gctgggcccg gggggtaacc ttcgccttat
     1441 ggagaacctt ctgctcccat gtctcgtacg tgccggagct ggtatcccgc gagatcacga
     1501 cgattttttt atccgcccct ccgacctctt tccagttggt gatcttcccc tggtaaatga
     1561 ggctcagttg ttcgatagtg aggtccgtga cagggttcgt gggatggacg atcgggacga
     1621 tggcgtctat tgccacacgg tgggcgacag gttcgacgcc cctttccccg gcgagcttta
     1681 cctcttccgg tttcatatcc cgtgaagagt tggctatgtc ggtagacttg tctatgaggg
     1741 cctttattcc gtccccggac cctcccgccg ataacgagat gttgatgtcg ggacgtttct
     1801 tcatgaaggc ttcagccgcc gcctgcgcga tgggaagcac ggttgtcgac cccttgatca
     1861 caacggtttc agcggcaacg gcgaaaaccg gggtgaaaaa aagcgccacg agcgtcgcgg
     1921 caatacattt aagggttctg gacattttta ctttcctccc tgttatgatt ttggggaagg
     1981 ataaggcacc gttgttacag tttgattaca acctaataaa gaaatgatga agtcctaaga
     2041 ggagggtttc tgcgcgaggt aaaggtgaca gacgccgaag gacagaggta cggaccgcga
     2101 aactgtgagg ccggagttgc aaagtagttc ttcaacctgt cgcggagcat aaaagtcgag
     2161 aacggaatcc
//
LOCUS       DAOTMX010000296         2162 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_39609_length_2162_cov_3.004746, whole genome shotgun sequence.
ACCESSION   DAOTMX010000296 DAOTMX010000000
VERSION     DAOTMX010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39609_length_2162_cov_3.004746"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="P5573_07905"
     CDS             complement(<1..534)
                     /locus_tag="P5573_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRT28023.1"
                     /translation="MSGPEYVQYGGDTTCIEVRTKNDEIIIIDAGTGLRRLGKQLIAE
                     KRFHYNLIFTHAHWDHLIGFPFFRPLYMRQTRIKVHGCPFAMDYVHTMLSRVMSPPNF
                     PVSFTDIKAHIEMDPLCLERFVIDSVTVSPIRLSHPNQGSGYKFVEDGRTFVFLTDNE
                     LDYRHPGGLDAGAYRDFC"
     gene            813..1325
                     /locus_tag="P5573_07910"
     CDS             813..1325
                     /locus_tag="P5573_07910"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HRT28024.1"
                     /translation="MGLLFALLLPDGGVWGKAYYRWTDEQGTVQISDEPPTRGKYEKI
                     IIPDRRETPSAETIGEKPREEAAADDDPVKRRQEAVVKELEEMVAHYSEVGGITAGKM
                     EALKEAVVIVKKAKMDGTEKDDEFYLKIEELVRAIKDHTMIIGRVHRLLREAKAMKGF
                     PVPPPEEGTE"
     gene            complement(1386..1667)
                     /locus_tag="P5573_07915"
     CDS             complement(1386..1667)
                     /locus_tag="P5573_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT28025.1"
                     /translation="MAKAKVGDRLSCGVCGLVVTVDEACGCASIEELVCCGEVMKKGE
                     AAARRARKAAAAAAAAPAVKKTAVKEAKPAAKKKAVAKTTAKKTVAKKK"
     gene            1879..>2162
                     /locus_tag="P5573_07920"
     CDS             1879..>2162
                     /locus_tag="P5573_07920"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRT28026.1"
                     /translation="MKKSCAKGVGMAENIFNLTKEDGVGVVTFAVPGDAMNTWSEAAI
                     ASYRAVLEELEEEKELTGVLFISGKPENFFAGADLKMIGGMSDPESVKAAL"
BASE COUNT          501 a          584 c          602 g          475 t
ORIGIN      
        1 gcagaaatcc ctgtaagccc cggcgtcgag ccctcccgga tgtctgtagt cgagctcgtt
       61 gtctgtcagg aagacaaacg tccggccgtc ttcgacgaac ttgtaaccgc ttccctggtt
      121 gggatggctc aggcggatgg gggaaacggt cacggagtcg atcacgaagc gctcgaggca
      181 cagcggatcc atttcgatgt gggcctttat gtcggtgaag ctgaccggga agttgggggg
      241 ggacatcact cgggagagca tcgtatggac ataatccatc gcaaagggac acccgtggac
      301 cttgatcctt gtctgccgca tgtaaagggg cctgaaaaag gggaagccga tcagatggtc
      361 ccagtgggca tgggtaaaga tgagattgta gtgaaagcgc ttctcggcga tcaactgctt
      421 tccgagccgg cgcaggccgg ttcccgcgtc gatgatgatg atttcgtcgt tttttgtcct
      481 gacctcgatg caggtcgtgt cgcccccgta ctgtacgtat tccggccctg acacgggaat
      541 agaccctctt gatccccacg acttaacgaa cacgggccac cctttccaac atgggattga
      601 gattgtctga acgccaaaat taagatatat gaacttgttg taaggcatgg tgcggtgcgc
      661 cacatcaccg gaccttaggt tattcctatt atagtgaggc cgagttgtca acctatcaaa
      721 aggggatcct cctgaagcat cctgccggaa aacaccccga acgaaaggtg aaaagttcta
      781 aaatgaggcg gactcttctc acgtttgttg cgatgggact tctcttcgcg ctgcttctcc
      841 cggacggcgg ggtatggggc aaggcttatt accggtggac ggacgagcaa ggaacggtcc
      901 agatcagtga cgaacccccg acgaggggga aatacgagaa gatcatcatc ccggaccgcc
      961 gggaaacccc gtccgccgag accatcggag aaaaaccccg ggaggaagcg gctgcggatg
     1021 acgaccccgt caaaaggcgc caggaagcag tcgtgaagga actcgaggag atggtcgccc
     1081 actattccga ggtcggcggg atcacggcgg ggaagatgga agcactgaag gaagcagtgg
     1141 tgatcgtaaa aaaagccaag atggacggta ctgaaaagga cgacgaattt tacttaaaga
     1201 tagaagagct cgtacgggcc atcaaggacc atacgatgat catcggccgc gtccacaggc
     1261 ttctcaggga ggccaaagct atgaagggct tccctgtgcc gcctccggaa gagggaacag
     1321 agtaaaaaca gttattgctc ggaaaagacc ggccttaacg attgggagaa gtttgtccag
     1381 atctcttact tcttcttcgc caccgtcttt tttgccgtcg tcttggctac agcctttttc
     1441 tttgccgccg gtttggcttc tttcaccgct gtctttttca cggccggcgc cgccgctgca
     1501 gccgccgcgg cctttctcgc ccttctggcc gctgcctctc cctttttcat cacttcaccg
     1561 cagcacacca gctcttcgat cgacgcacaa ccacatgcct catccaccgt gactaccaaa
     1621 ccgcacaccc cacagctcag tctatctccc acctttgctt ttgccatgac tccctcctgc
     1681 ggtcaatgag ttgtcaaccg tccggcgaca atgaacgccg cgcactttca ggttcctgta
     1741 tatgaaatgt cgcaaatttt caagatattt tcttccgttc cctcatcgtc tgacgggcac
     1801 accacgcagg aatgaacggg tcatcattat ttcttgacgc aaccgagtta ttctgcaaat
     1861 ctttattctc ctttgggaat gaagaaatct tgcgcgaaag gagtgggtat ggccgagaat
     1921 atattcaacc tcacaaaaga ggacggggtc ggtgttgtga cgttcgctgt gccgggtgac
     1981 gccatgaaca catggagcga ggccgcgata gccagttatc gcgccgtgct cgaggaactc
     2041 gaggaggaga aggaattgac aggcgtcctt ttcatttccg ggaaacccga aaattttttc
     2101 gccggcgccg acctgaagat gatcggaggg atgtcagacc cggaatccgt gaaggcggcc
     2161 ct
//
LOCUS       DAOTMX010000297         2159 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_39669_length_2159_cov_3.791350, whole genome shotgun sequence.
ACCESSION   DAOTMX010000297 DAOTMX010000000
VERSION     DAOTMX010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39669_length_2159_cov_3.791350"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="P5573_07925"
     CDS             <1..709
                     /locus_tag="P5573_07925"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HRT28027.1"
                     /translation="GNTAENIAAKYGISREEQDKLAYESHRRARAAIASGAVADEIVP
                     VVIPQKKGDPKIFSVDERPMETTLEKMAKLPPVFRKDGTVTAGNASGINDAAAAVVVM
                     SEKKAKELNLKPLARIKGYASGGVEPSYMGLGPIPAVRKLFKKFGITMKDIDLVELNE
                     AFAVQALGCIKELEIDLDKCNLNGSGISIGHPIGCTGARITYTLAMQMKKKNLKLGLA
                     SLCIGGGQGMAMLLENV"
     gene            843..1700
                     /locus_tag="P5573_07930"
     CDS             843..1700
                     /locus_tag="P5573_07930"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HRT28028.1"
                     /translation="MAVKTFGVVGAGQMGNGIAQVAAYPGGLNVIMNDIADQFVDRGF
                     DTISKNLDRLVAKEKLTADQKKEILGRIKKSTNLEDMKDADFVVEAATEREDLKLNIF
                     KKLDEVCKPGVILATNTSSIPITRIAGATKRPGLVIGMHFMNPVPVMRLVEIIRGIAT
                     AQETYNLTESLAKQFGKVPVECNDFPGFISNRILMPMINEAFYALMEGVGQPKAIDEI
                     MVLGMNHPMGPLALADLIGLDTCWAIMDVLYKGFGDSKYRPCPLLKKYVDAGWLGRKT
                     GKGVYDYSK"
     gene            1730..>2159
                     /locus_tag="P5573_07935"
     CDS             1730..>2159
                     /locus_tag="P5573_07935"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRT28029.1"
                     /translation="MEYKNLLFAVEDGVAIITFNRPKALNAMNSETMKELYDAVIRCK
                     NDETIKAIILTGSGEKAFVAGADISEMKDLRPKEALAFMELGHETLRALETLPKPSIA
                     AVNGFALGGGTEISMACDMRFASETARFGQPEILIGLIPGW"
BASE COUNT          555 a          611 c          624 g          369 t
ORIGIN      
        1 gggcaacaca gcggagaata tcgcggcaaa gtacggtatt tcgagggaag agcaggacaa
       61 gcttgcgtac gagagtcaca gacgcgcgag ggcggccatc gccagtggag ccgtcgccga
      121 tgaaatcgtc ccggtcgtca tcccccagaa gaagggtgac ccgaagatct tcagcgtgga
      181 cgagaggccg atggaaacaa ccctggaaaa gatggctaag cttccacctg ttttcaggaa
      241 agatggcacc gtcaccgcag gaaacgcctc cggcatcaac gacgcggccg cggcggtcgt
      301 ggtcatgagt gagaagaagg cgaaggagct gaacctaaag ccccttgcca ggatcaaggg
      361 atacgcctcc ggcggagtcg agccctccta tatgggtctc gggcccatcc ccgccgtaag
      421 gaagctcttc aaaaagttcg gcatcacgat gaaggacatc gacctcgtag agctgaatga
      481 agccttcgcc gtacaggcgc tcggctgcat caaagagctc gaaatcgacc tcgacaaatg
      541 caacctcaac ggaagcggca tctccatcgg gcaccccatc ggatgtacgg gcgcccggat
      601 cacttacaca ctggcgatgc agatgaagaa gaaaaacttg aagctcggcc tcgcctccct
      661 ctgcatcggc ggcggacagg gtatggccat gcttctcgag aacgtttaac cgtcacgatt
      721 tacggcgggg aggccgagac ctccctgccg taaattccac atttcgtctt ataggccgta
      781 gtgacaccga taagggtcgc agcagagctc ttaaactcaa atcaagtaag gagggacaga
      841 ctatggcagt aaaaacgttc ggcgtagtgg gagccggcca gatgggtaac ggcatcgccc
      901 aggtagcggc gtacccaggc ggactcaacg tcatcatgaa cgacatcgca gaccagttcg
      961 tcgacagggg cttcgacacc atttccaaaa acctcgatag gctggtggca aaggaaaaat
     1021 tgacggcgga ccagaagaag gaaatcctgg gccgtatcaa gaaaagcacc aatctagaag
     1081 acatgaaaga tgccgatttc gtcgtcgagg cggccaccga aagagaagac ctcaagctca
     1141 acatcttcaa aaaactcgac gaagtatgca aacccggggt aatcctggct acgaacacct
     1201 cgtccatccc gatcacgcgg atcgccgggg ccaccaagag gcccggactg gtcatcggga
     1261 tgcacttcat gaaccccgtc ccggtcatga gacttgtcga gataatccgg ggcatcgcga
     1321 cggctcagga aacttacaac ctgacggaga gcctggcgaa gcagttcggc aaggtacccg
     1381 tggagtgcaa cgacttcccg ggcttcatct ccaaccggat cctgatgccc atgatcaacg
     1441 aagccttcta tgccctgatg gaaggcgtcg gccaacccaa ggctatcgac gaaatcatgg
     1501 tcctcggcat gaaccacccc atgggaccgc ttgcgctggc ggacctgatc ggcctcgata
     1561 cctgttgggc catcatggac gtcctctaca agggtttcgg ggattccaaa taccgtccct
     1621 gcccgctttt gaaaaagtac gtagacgccg gttggctggg cagaaagacc ggcaagggtg
     1681 tttacgacta cagcaagtaa ataaaaacga cagataggga ggcgggaaaa tggaatacaa
     1741 gaacctattg tttgccgtcg aagacggggt ggcgatcatc actttcaacc ggcctaaggc
     1801 cctgaacgcc atgaattcgg agaccatgaa ggagctctac gatgccgtca tccgctgcaa
     1861 gaacgacgag accatcaaag cgatcatcct gaccggttcc ggggaaaagg cattcgtcgc
     1921 cggcgccgat atctcggaga tgaaagacct gcggcccaaa gaggcccttg cgttcatgga
     1981 attgggacat gaaacccttc gggccttgga gaccctgccc aagccgtcga tcgcggcggt
     2041 aaacggcttc gccctcggcg gcggtaccga gatctcgatg gcctgcgaca tgcggttcgc
     2101 ctcggagacc gcccgcttcg gacagccgga aatactcatc gggctgatcc ccgggtggg
//
LOCUS       DAOTMX010000298         2155 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_39769_length_2155_cov_3.734286, whole genome shotgun sequence.
ACCESSION   DAOTMX010000298 DAOTMX010000000
VERSION     DAOTMX010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39769_length_2155_cov_3.734286"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            255..635
                     /locus_tag="P5573_07940"
     CDS             255..635
                     /locus_tag="P5573_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRT28030.1"
                     /translation="MVSDINVTPLVDVVLVLLIIFMVTAPMLQMGIDVNLPKVEARPV
                     DVAEEKLVLTVNNRNEIFINAHKTSLADLGPKLENIFKTKIEKEVFLRADKEVPYGYV
                     VQVMSEVRKAGVDRLGMITEPVKE"
     gene            658..1356
                     /locus_tag="P5573_07945"
     CDS             658..1356
                     /locus_tag="P5573_07945"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRT28031.1"
                     /translation="MKGKDGGLEGFNRMIALSLMLHFFFLTILFFSSSLPARRWTFGP
                     VYTVDLVSLPSGYSDKGTSTSLLPEVAAIKTGRQADVLKKRSELAVPPIKRIAPAGRE
                     AGEVGRAIEGIRKKVEAPSGAQTRANQQGAVETSSAVNAYYQTLWARIRAEWALPRGI
                     LPSENLETVIDVTVSRSGAVTAISIEKSSGNQYFDQSALRAIRKASPFPPVPEDIRGS
                     SIELGIRFHSEQFR"
     gene            1397..>2155
                     /locus_tag="P5573_07950"
     CDS             1397..>2155
                     /locus_tag="P5573_07950"
                     /inference="COORDINATES: protein motif:HMM:NF015980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT28032.1"
                     /translation="MRDKLRVVSFVLVVLFVSLLPVYGKVYIDIDSPTIQKFPIAVAD
                     FKNLGQAPDRENLSAWFADRLGNALQLTGFFNVIQRKTFFEDPVKSGITAETIQFSDW
                     TVIGAESLIKGGFQLNGSDLTVEFRLFDVVQGRLLTGKRYTGKPGDREEMVLRFAEEV
                     LLQLTGERGVFDTKVAFAGKEGNLSEIYTVNFDGSGLKKLTNFRSLTLLPRWSPDGRE
                     MTFTSYRDGNPDVYLLDLISGKVRKLLEFSGLNLA"
BASE COUNT          505 a          531 c          617 g          502 t
ORIGIN      
        1 ccggccgtca ttttctacaa ctattaccta aacaaggttc gggccatcac ggtggagatg
       61 gacagctttg cctccgaatt cctcaacctc atcgagaggt attacgtcaa gaacgtctct
      121 tcctgatcat gtggctgaaa gagcccagcg gctctgacac ccattttttt cttgtgggag
      181 actttcaata ccttacggag ttttaggggg ttgtcgtgag atacaggaga aatcgcaata
      241 acggagacca tagattggtt tccgacatca atgtgacgcc tcttgtcgac gtcgttctgg
      301 tcctcctcat catcttcatg gtgacggcgc cgatgttaca gatggggatc gacgtgaacc
      361 tgccgaaggt ggaggctcgc cccgttgacg tggcggaaga aaagctcgtt cttaccgtca
      421 acaacaggaa tgaaatcttc atcaatgccc ataaaacttc gctggccgac ctggggccga
      481 agctcgagaa cattttcaag acgaagatag agaaagaggt gttcctcagg gccgataaag
      541 aagtgcccta cggatacgtc gtccaggtga tgtccgaggt caggaaagcc ggcgtcgaca
      601 ggctcgggat gatcacggag ccggtcaagg aatgaggaga tggctttttt ggggggaatg
      661 aagggaaagg atggcggtct cgagggcttt aaccggatga tcgccctgtc cctgatgcta
      721 catttctttt ttctgactat cctttttttc tcttcttctc tgcccgccag gcgatggacc
      781 ttcggcccgg tttacactgt cgacctcgtc agccttccct caggttattc ggacaaagga
      841 acgtcgacgt cattgctgcc ggaggtggcc gctatcaaaa cggggcggca agcagatgtg
      901 ttgaagaagc gctccgaact tgccgttcct cccatcaaga gaatcgctcc tgcaggaagg
      961 gaggctgggg aggtcggaag ggcgatcgag ggaataagaa aaaaggtaga ggcaccttcg
     1021 ggtgcccaga ctagggctaa ccagcaagga gccgtcgaaa cgagctctgc ggtgaatgct
     1081 tactatcaga ctttgtgggc gcgtataagg gcggaatggg ccttgccgcg gggaatcctt
     1141 ccgtcggaaa atctggagac ggtgatcgac gttaccgtgt cgaggtcggg tgcggtgacc
     1201 gcgatcagca tcgagaagag ctcgggaaac cagtacttcg accaatccgc tttaagggcg
     1261 atacgcaagg caagcccttt cccgcccgta cctgaagaca tccgggggag cagtatcgaa
     1321 ctgggcattc gattccactc cgagcagttt cgttagccga cattcgataa actaaagagc
     1381 cgaacagggg atacgaatgc gggataaact acgggttgtt tcttttgttc ttgttgtcct
     1441 ttttgtgagc cttctcccag tgtatggcaa ggtttacatc gacatcgatt ctcccacgat
     1501 acagaaattt cccatcgccg ttgcggactt caaaaacctg ggacaggcgc ccgacaggga
     1561 aaacctgagt gcctggttcg ccgacaggct cggcaacgcg cttcagttga cgggtttttt
     1621 caatgtcatt cagagaaaaa ctttttttga ggatccggta aaatcgggta tcacggcgga
     1681 aaccatccag ttttccgatt ggacggtcat cggtgccgaa tccctgataa aaggaggatt
     1741 tcagttaaac ggctcagacc tgacggtcga gttccgtctc tttgacgttg tccagggtag
     1801 gctgcttacc ggtaagcggt acaccgggaa accgggcgac cgggaagaga tggtgctccg
     1861 atttgccgag gaggtcttac ttcaactcac aggcgaaaga ggggttttcg acacgaaggt
     1921 cgctttcgcg gggaaggagg ggaacctttc cgaaatctat acggtcaatt ttgacggctc
     1981 gggcctgaag aaactgacga attttaggtc tttgacgtta ctcccccgat ggtctcccga
     2041 cggcagggag atgaccttca cctcttaccg agacgggaac ccggatgttt atcttctcga
     2101 cctgatcagc gggaaggtca ggaaactgct ggaattcagt gggctaaacc ttgcc
//
LOCUS       DAOTMX010000299         2144 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_40027_length_2144_cov_3.205840, whole genome shotgun sequence.
ACCESSION   DAOTMX010000299 DAOTMX010000000
VERSION     DAOTMX010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40027_length_2144_cov_3.205840"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1128
                     /locus_tag="P5573_07955"
     CDS             <1..1128
                     /locus_tag="P5573_07955"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRT28033.1"
                     /translation="ISIVGLLITVFGVARIIVDIPAGELTNRLGRRPVLIIGPVIQAI
                     SSVGCGLAVDYWMLLFFRFLQGIGSAMYTTAANVMLADISSPVNRGRLMSYYQGSLLL
                     GTGLGPTMGGFIAQYFGLAAPFFVYAFLVFFAGLWAYLRLPETRPQSTTPAAAPVADK
                     SGLSPSTSPSGEVRALLRNLDFILVSTVSFGIFFTRQGALNEILPLLGSGRLGLREGQ
                     IGLALTLVAIFQFMTIFPSGRLADRFGRKAVISPGCLVAALSLVLMAQSQSYWFLMFS
                     CVVMGVGIGLSGPTPSAYVADITPRESYGAAMGTYRAISDLGFVIGPIILGWLADTEG
                     LRFSLLFNSLFLFLIVLIFHLRAKEPTRRYSGSTELGEGEE"
     gene            1161..1688
                     /locus_tag="P5573_07960"
     CDS             1161..1688
                     /locus_tag="P5573_07960"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HRT28034.1"
                     /translation="MKNIVGFIHRVATGKRHTRIVLAPVFAAFFLFLVMLTVFLALKA
                     DEVFNLSAPVGYPWSCVLSLPLLAVGVFLWLWSVLHFIKVKGTPIPVSPPPTLVDYAY
                     TRNPMLTGVFFLLFGTGFAVGSPSLLLFFAPVFVACSVLVFKFIEEPELEKRFGEAYR
                     EYRRKTPMLIPKLFR"
     gene            1910..>2144
                     /locus_tag="P5573_07965"
     CDS             1910..>2144
                     /locus_tag="P5573_07965"
                     /inference="COORDINATES: protein motif:HMM:NF026110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT28035.1"
                     /translation="MVSRSIYITESDKMRLEEIIAVASEFSSHTRKDLEALAQELARA
                     EIVDPKEVRPDVVTMNSRVILQDIDTDEEMNYAL"
BASE COUNT          429 a          581 c          591 g          543 t
ORIGIN      
        1 atctccattg ttgggttgct catcactgtc ttcggagtag cccggatcat cgtcgatatc
       61 ccggcaggag aactgactaa taggttgggg cgacggcctg ttttgatcat cggcccggtg
      121 atacaagcta taagctcggt aggttgcggg ctggcggtcg actactggat gcttctcttt
      181 ttccgtttcc tccagggtat aggttcggcg atgtacacca cggcagctaa tgttatgctt
      241 gccgatataa gctcaccagt taaccggggg cgattgatga gttactacca ggggagcctc
      301 cttctcggta ccggattggg gccgaccatg ggaggattca tcgcccaata ctttgggctg
      361 gcggccccct tttttgtcta tgcctttctc gttttttttg ccggcctctg ggcatacctg
      421 cgtctccccg agacacgccc gcagtcgacc accccggcgg cggcaccagt tgcggacaaa
      481 tcaggcctgt cgccgtctac ttcgccgtcg ggtgaagtga gggcactgct gcgaaatctt
      541 gatttcatcc tagtctctac cgtatccttc ggcatcttct tcacgcgcca gggggcgttg
      601 aacgagatcc tacccctcct cggcagcggc cgtctcggct taagagaagg acagatcggc
      661 ctggcgctta ccttggttgc aatcttccag ttcatgacca tcttcccctc gggcaggctt
      721 gccgaccgtt tcggcaggaa ggcggtcatt tctccgggat gcctcgtcgc cgccctttcc
      781 ttggtgctca tggctcaaag tcaaagttac tggtttttaa tgttttcctg cgtggttatg
      841 ggggtgggca taggcctgtc aggcccgact ccgtcggcct atgttgccga tatcaccccc
      901 agagagagct acggcgcagc tatggggacg taccgcgcca taagcgacct ggggttcgtt
      961 atcggtccca tcatcctggg atggctggcc gataccgaag ggctcagatt ttcgctgctc
     1021 ttcaatagct tgtttctctt cctgatcgtc ctcatctttc atttgcgggc gaaggaaccg
     1081 acccgacggt atagtggttc gactgaactg ggcgaagggg aggagtgatc agacgggcct
     1141 tgattttatg gaggtaagaa atgaaaaaca tcgtcggatt catccatcga gttgcaacag
     1201 gaaaacggca tacccggata gtccttgcgc cggtattcgc cgccttcttc cttttcctgg
     1261 ttatgctgac tgtattcctc gcactgaagg ccgacgaggt cttcaacctc tccgcgcccg
     1321 tcggttatcc ctggagctgt gtcctttctc tgccgctgct tgcggtcggt gtttttctgt
     1381 ggttatggtc ggtcctgcat ttcataaaag tgaaaggaac ccccatacct gtcagcccac
     1441 cccctaccct tgtcgactac gcctacacga gaaacccgat gctcacaggc gtatttttcc
     1501 ttctcttcgg gaccgggttt gccgtagggt ccccgtccct tctgttgttc tttgcaccgg
     1561 tctttgtggc ctgcagtgtt cttgtgttta aattcatcga agaacccgag ctggagaagc
     1621 gcttcggtga ggcctaccgt gaatacagaa gaaagacccc gatgctgatc ccgaaactat
     1681 ttcgataagg tatccctact cggcaccatc tcgagttgaa gaaagggtaa agggactcgt
     1741 ttaacttatt gacttgcata gaaacgggga atcatctatt ggtatactat gatgtccggc
     1801 aggagataga caagaatctt tgcctgaccg gcacgttcgg cggaccaccg gattctccgg
     1861 tgcacgccgc tgtctgtatc ggtgccgaaa agaaagagga ggagaggtca tggtgtccag
     1921 aagcatatac atcaccgaaa gcgacaagat gcgactggaa gaaatcatcg cggtggccag
     1981 tgagttcagc agccacacca gaaaggattt ggaggccctg gcgcaggaac tcgcgagggc
     2041 ggagatcgtg gacccaaagg aagtacgccc cgacgtagtt accatgaatt cgagggttat
     2101 tctccaggac atcgataccg atgaagagat gaactatgcc ttgg
//
LOCUS       DAOTMX010000300         2128 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_40406_length_2128_cov_3.981669, whole genome shotgun sequence.
ACCESSION   DAOTMX010000300 DAOTMX010000000
VERSION     DAOTMX010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40406_length_2128_cov_3.981669"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="P5573_07970"
     CDS             complement(<1..407)
                     /locus_tag="P5573_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HRT28036.1"
                     /translation="MASLIIKVLSSTVIGIESFPVDVEVDISPGLPQFSTVGLPDTAV
                     KESRDRIRAAVRNSGYEFPPDRVTVNLSPADIKKEGTSFDLPIAVGVLAAQELIEKKR
                     LPEFMIIGELSLDGSVKGVHGALPAAFRAKQDG"
     gene            613..1110
                     /gene="moaC"
                     /locus_tag="P5573_07975"
     CDS             613..1110
                     /gene="moaC"
                     /locus_tag="P5573_07975"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018465116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HRT28037.1"
                     /translation="MTEFTHLDDKGKPRMVDVSVKETTLREATARGKVLMRPETAQAV
                     QAGGFAKGDVLTVAKVAGIMAAKKAGELIPLCHPIEITGVDISFSVDGLRGEVGIEAT
                     VRTVGRTGVEMEALTAVSVAALTIYDMCKSADRAMVLSDICLTRKSGGRSGTFVREPG
                     EDTER"
     gene            1152..1664
                     /locus_tag="P5573_07980"
     CDS             1152..1664
                     /locus_tag="P5573_07980"
                     /inference="COORDINATES: protein motif:HMM:NF022216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HRT28038.1"
                     /translation="MEDVYKIKKGLVVALMVDTALLAVLLAVSFFKGNSPVEKVVLSV
                     ILVPLLLVTVESNCRKVTVSEKGLSIRKLFRKREFLWGDITDVGAVILRNRVYLVLTT
                     TKGFHIISNAYDDFPALLRGIVSHVEAERVEEQVKALVERPVTKVSDVVSAWAGAVVL
                     LVVIYIRVFL"
     gene            1690..1986
                     /locus_tag="P5573_07985"
     CDS             1690..1986
                     /locus_tag="P5573_07985"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRT28039.1"
                     /translation="MARKEPAKGAGSEKVKPSCLAVEVLLEEKIEDVVNFLGSSEGSR
                     SQLYDDILALVERCLIRIALKRTNNVKTSAAAFLGINRNTLHKKMDQLNIPYRD"
BASE COUNT          456 a          546 c          664 g          462 t
ORIGIN      
        1 atcccgtcct gtttcgcccg gaaggcagcc gggagcgccc catggactcc cttcacgctt
       61 ccgtcgagtg atagctctcc gatgatcatg aattcgggca gccttttttt ctcgatcaac
      121 tcctgggcgg cgaggacacc cacggcgatg gggaggtcga aacttgttcc ctctttcttg
      181 atgtcggcgg gagagaggtt gacggtcacg cggtcggggg ggaattcgta gccggaattc
      241 ctgactgcgg cgcggatacg gtccctgctc tccttcacgg ccgtgtcggg cagtccgacc
      301 gtggagaact ggggaaggcc gggcgagatg tccacctcga cgtccacggg gaaggactct
      361 atcccgatca cggtactgct caagaccttg atgatcaaag aagccatcct ccagaagatt
      421 tcgggaaacc cggaagcggg gtcaaagaca ggcaaagggc gaccctgaag ataacgaata
      481 gcgggaaata attcaagaac tttgttggtg cgggacgtcc ttgacaccgg agaaaggtct
      541 gtgttaagta acttcccact tttcgactgc tcattctttc ctttaagtct ccaacgaata
      601 ggcgatcccc cgatgaccga atttacccac ctcgacgata agggtaaacc gaggatggtt
      661 gacgtatccg tgaaggagac aacccttcgg gaggcaaccg cccgcggtaa ggtcctgatg
      721 aggccggaga cggctcaggc cgttcaggcg ggaggttttg cgaaaggcga cgtgcttacc
      781 gttgcgaagg tcgccgggat aatggcggcg aagaaggcgg gcgagctcat ccctctctgc
      841 catccgatcg agatcacggg cgtcgacatc tctttttccg tcgacggcct gagaggtgag
      901 gtcggcatcg aggccaccgt gaggacggtg gggaggacgg gcgtggagat ggaggcgctg
      961 acggcggtca gtgtcgcggc actgacgata tatgacatgt gtaaatccgc cgaccgcgcc
     1021 atggtccttt ccgacatctg tcttacccgg aagagcgggg gacggagtgg gacgttcgta
     1081 agggagcctg gggaagatac tgaaagatag gcagtgcggt gcggaaggaa taggtttcgg
     1141 ggtatcgagg tttggaagac gtttacaaga taaaaaaggg cctcgtcgta gcccttatgg
     1201 tcgacactgc gcttctcgct gtcctgctgg cggtctcctt tttcaaagga aactcccctg
     1261 tcgagaaggt tgtcctgtcg gtcatcctgg tccctctcct cttggtgacc gtggagtcga
     1321 attgccggaa ggtgactgtc agcgagaaag gcctctccat ccggaaactt tttcgcaaga
     1381 gggaatttct ctggggagac atcaccgacg tgggggccgt tatcctgagg aacagggtct
     1441 atctcgtgtt gacaacgacc aaggggtttc atatcatctc taacgcctac gatgattttc
     1501 ccgccctgct gagagggata gtcagccatg tcgaggcgga acgggtcgaa gagcaggtga
     1561 aggctttggt cgagcgaccg gtcacgaagg tttccgacgt cgtttcggcc tgggcgggcg
     1621 ccgttgtgct tctcgtcgtc atctacatca gggtttttct ctaatcaccc tttgaagagg
     1681 gactgaacta tggccaggaa agagccggca aaaggggcag ggtcagagaa ggtcaagccc
     1741 tcgtgcttgg ctgtggaggt gctgctggag gaaaagatcg aagacgtagt gaacttcctc
     1801 ggctcgtcgg agggttcaag aagccagttg tatgacgata tcctcgccct ggtggagagg
     1861 tgtctcatcc ggatcgccct gaaacgcacc aacaatgtca agacttctgc cgccgccttc
     1921 cttgggatca accggaacac cctgcacaag aagatggatc aactgaacat cccgtaccgg
     1981 gactgaactg gtatccttcc ccggagaatc acggcgctga tgtctttgtc ctatcgttct
     2041 caggacgcgg gaccggttgt tgtgatcgca aaactcacag ggagattcac agctttaatg
     2101 tgatggtagt cggtgtgttg gcgctcca
//
LOCUS       DAOTMX010000301         2107 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_40961_length_2107_cov_3.876706, whole genome shotgun sequence.
ACCESSION   DAOTMX010000301 DAOTMX010000000
VERSION     DAOTMX010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40961_length_2107_cov_3.876706"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..1796)
                     /locus_tag="P5573_07990"
     CDS             complement(66..1796)
                     /locus_tag="P5573_07990"
                     /inference="COORDINATES: protein motif:HMM:NF014598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HRT28040.1"
                     /translation="MTTTVVIIIAFAVYMALYWIYGKKIERDVVRASDAVDAPSKRLY
                     DGIDYVPANKYVLFGHHFASIAGAGPIIGPAMAMCWGWLPGLLWVWFGNVFIGAVHDY
                     LALMASIRYDGKSVQFVASDLFTKRSGTAFYWLVFFLLILVVAAFADVVASMFAKTGE
                     IASASVWMTLVAILLGQMIYRWKINLAASTVVGVVLLAAAIWAGTQTPFLVKHEIWLL
                     VLFAYIIIAAAIPVNILLQPRDYLNSFLLYFGLALGFAAAVFTFKGFEAPAVSSFSPI
                     LVGGKATPFWPAIPLIIACGSLSGFHSLVASGTSSKQLEKESDALFVGYGSMFTEGFL
                     STLIICAIGGFGFTALQNAAAAANQPLTLTAENWGAMYTKVTGAFKLVPANMVVQSYA
                     DMVAASFLAWIPTLLVKVIAGLWLSAFALTTLDTTNRLARYCLVEMCEPFRTSAEGLY
                     RAITFRWTASLLPAALGVYLAWSKNFTIVWPAFGAANQLIASLALMTGAAYVAKRLKS
                     KFAVIATVPAFLLWITVTAAIIWFMYIVMPDNIAKNPGQGWTVQIIMGIMLILNLLFI
                     WDFIRLRKTE"
BASE COUNT          502 a          540 c          680 g          385 t
ORIGIN      
        1 acctttgaga gacggcttaa aaccgcttcc aaaggtctcc ccgttttacg cgataggaat
       61 ctctctcatt ccgttttacg cagtcggata aagtcccaga tgaagaggag attcagaatg
      121 agcatgatcc ccatgatgat ctggaccgtc cagccctgtc cggggttctt ggcgatgttg
      181 tcgggcatga cgatatacat gaaccagatg atggccgcgg tcacggtgat ccagagcagg
      241 aatgccggaa cggtggctat aacggcgaat ttcgatttga gtctcttcgc cacgtaggcc
      301 gctcccgtca tgagggcaag tgacgcgatc agctggttcg ccgctccgaa agcgggccag
      361 acgatggtga agttcttgct ccaggcgaga taaacgccca gggccgccgg taagagggag
      421 gccgtccacc tgaaggtgat ggccctgtag aggccctcgg ccgacgtcct gaagggttcg
      481 cacatctcga cgaggcagta gcgggcgagg cggttcgtcg tgtcaagggt ggtgagggcg
      541 aaagccgaga gccacaaccc cgcgatcacc ttcacgagga gggtcggtat ccaggcgagg
      601 aagcttgccg ccaccatgtc cgcgtaggac tgcacgacca tgttggcggg aacgagcttg
      661 aaggctcccg tcaccttcgt gtacatcgcg ccccagttct ccgccgtgag ggtgaggggc
      721 tggttcgcgg cggcggcggc gttctgcagg gccgtgaagc cgaaaccacc gatggcgcag
      781 atgataaggg tcgacaggaa cccttccgtg aacatggaac cgtagccgac gaagagggcg
      841 tcggactcct tctcgagctg tttggaagac gtaccggagg ccaccaggga gtggaagccc
      901 gagagggacc cgcaggcgat gatgagcgga atggccggcc agaagggggt ggctttgccg
      961 ccgacgagaa tcggcgagaa gctggaaacc gcaggcgcct cgaacccctt gaaggtgaag
     1021 acggcggccg cgaagcccag agcgagaccg aagtagagca ggaaggagtt caggtagtcc
     1081 cgcggctgca gcaggatgtt cacggggatg gcggcggcga tgatgatgta ggcaaagaga
     1141 accagaagcc atatttcgtg cttcacaaga aagggggtct gtgtccccgc ccagatggct
     1201 gcggccagga ggaccacgcc gacaacagtc gatgcggcca ggttgatctt ccaccggtag
     1261 atcatctggc cgaggaggat ggccacgagg gtcatccaaa cggaggccga ggcgatctcg
     1321 ccggttttcg cgaacatgct cgccacgacg tcggcgaagg cggccacgac gaggataagc
     1381 aggaagaaga cgagccagta gaaggccgtt ccggagcgct tcgtgaagag gtccgaggcg
     1441 acaaactgca cggacttccc gtcgtagcgg atcgaggcca tgagggccag gtagtcatgg
     1501 acggcgccga tgaatacgtt tccgaaccac acccagagaa gacccgggag ccagccccag
     1561 cacatggcca tggcggggcc gatgatcggt cccgcgccgg caatggaggc gaagtggtgt
     1621 ccgaagagga cgtatttgtt ggcgggcacg tagtcgatgc cgtcgtagag acgtttcgag
     1681 ggtgcatcca cggcatccga tgcccggacg acgtcacgct cgatcttctt gccgtagatc
     1741 cagtagagtg ccatgtaaac agcgaaagcg ataatgataa caacagtggt ggtcatctac
     1801 atctcctcct ttcaaagatt tgggaacagg tcagataacg gttgaaagaa aacgtcggta
     1861 aaccctatgc acgaggtatt taattacgcc tgttgccctg taaagtcaaa tggtttgtcg
     1921 cttgacaaag aaggtttttg gagtatttta aaaggaggtt tttggggtga tttaggtcgg
     1981 atcaacccgt caattttcgg cccaaagccc gagggagccg gccatcgaga attgaatcat
     2041 aaagaatatt agaaacttga tagcataaaa tacacgttac ctgttctttg aaaaagcaga
     2101 gagcatc
//
LOCUS       DAOTMX010000302         2100 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_41126_length_2100_cov_3.371149, whole genome shotgun sequence.
ACCESSION   DAOTMX010000302 DAOTMX010000000
VERSION     DAOTMX010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41126_length_2100_cov_3.371149"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="P5573_07995"
     CDS             complement(<1..333)
                     /locus_tag="P5573_07995"
                     /inference="COORDINATES: protein motif:HMM:NF014027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HRT28041.1"
                     /translation="MKKKDYLRSKVQHIDITTLDAKDIVEAMRGMSFTARDLATAADI
                     YDRMLRDSRCSIILTIAGSTSAAGCMQIYADLVKYNMVDAVVATGASVVDMDFFEALG
                     FNHYQGTPW"
     gene            568..1896
                     /gene="xseA"
                     /locus_tag="P5573_08000"
     CDS             568..1896
                     /gene="xseA"
                     /locus_tag="P5573_08000"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HRT28042.1"
                     /translation="MKEILSVTELTLRIKFLLEANFEVFWVEGEVSNLRRPGSGHAYF
                     TLKDETCQIRAVIFRTDLRSLSFELEDGMQVILRARLNVYEPRGEYQLIADAVEPKGL
                     GALQKAFEQLKARLQEEGLFDAKHKKQIPAVPRNIGVVTSPTGAVIRDILNITGRRFP
                     SVNILIVPVRVQGLDAPMEIVEAIENLNAWGKVDVIILARGGGSLEDLAPFNDERVAR
                     AVFRSRIPVISAVGHEIDFTIADLVADLRAPTPSAAAELAVPDRRELRESVGALYLRM
                     CRVCRRDLEDRREKFSFLLERLRDPRRLIADMRLRIDDRSNQLATALANRLAAVKGRF
                     SLLKNHLRLLSPAATIRERRFIVENFHKNMVAAWFLFRERVGKRLATDTAVLDSLSPL
                     AVLGRGYGIVRRLPEGTILRETGSIAAGDDVMVKLYVGRFQARVVKIEER"
     gene            1901..>2100
                     /gene="xseB"
                     /locus_tag="P5573_08005"
     CDS             1901..>2100
                     /gene="xseB"
                     /locus_tag="P5573_08005"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HRT28043.1"
                     /translation="MAGEKFEEALAKLEEIVKRMESGELTLDESLEAFEEGVRLSRLC
                     AERLDEAERRVEALLKTTEGFE"
BASE COUNT          477 a          554 c          619 g          450 t
ORIGIN      
        1 ccaaggagtc ccctggtaat ggttgaaacc gagggcctcg aagaaatcca tgtccactac
       61 ggaggccccc gtggccacga cggcgtcgac catgttgtat ttcacgaggt cggcgtatat
      121 ctgcatacag ccggcggcac tcgtgctgcc ggcgatcgtc aggatgatgg aacaccgaga
      181 gtcccgcagc atcctgtcgt agatatccgc tgccgtcgcc aggtcccggg ccgtaaaaga
      241 catccctcgc atggcctcga cgatatcctt cgcgtctaag gtcgttatgt cgatatgctg
      301 aactttcgat ctcaagtaat ctttcttctt catgatcccc ctgcagctcc tcgatgattt
      361 ctcgctttgt tcgaaatgac gtgccttaag aatttacgga gcacgataat aataagcggc
      421 atcaatgacc gtgttttgcc tgttttttac aggctcatac tatttaaacg atgagcctct
      481 gaatgtcaaa agcttaatcg aagagggaac caggcccgga aaataagtac ttgcaagggg
      541 agggttcctt ttgttaaacg gtggtatatg aaagagatcc taagcgttac ggaattaacc
      601 ctcaggatca aattcctgct cgaagcaaat tttgaggtct tctgggttga gggagaggtc
      661 tccaacctgc gccggccggg ctccggccac gcatatttca ccctgaaaga tgagacgtgt
      721 cagataaggg ctgttatttt ccggacggac ttgaggtctc ttagcttcga gctcgaggac
      781 gggatgcagg tcatcctccg ggcgcgcctc aacgtctacg agccgagggg agaataccag
      841 ctcatcgccg atgccgtgga gccgaaaggc ctcggcgccc ttcagaaggc cttcgagcag
      901 ctcaaggccc gcctccagga ggaggggctt tttgatgcta agcacaagaa gcagattcct
      961 gcggtccccc ggaatatcgg ggtcgtcact tcgccgacag gcgccgtaat ccgcgacatc
     1021 ctcaacatca ccggccgccg ctttccctcc gtgaatatcc ttatcgtgcc cgtgcgtgtc
     1081 cagggcctcg acgcccccat ggagatagtc gaggccatcg agaacttgaa cgcctggggg
     1141 aaggtcgatg tcataatcct ggcaaggggc ggagggtcgc tcgaggacct tgccccgttc
     1201 aacgacgaaa gggtggcgag ggctgtattc cgctcccgta tccctgtgat ctcggccgta
     1261 ggccatgaaa tcgattttac catcgccgac cttgtggccg acctcagggc gccgactccg
     1321 tctgctgccg ccgaactggc ggtgcccgac cgaagggaac tccgtgaatc ggtcggagcc
     1381 ctatacctgc ggatgtgccg cgtatgcagg cgcgacctgg aagaccgaag ggaaaagttc
     1441 tcatttctcc tggaacgatt gagggaccca cgacgcttga tagccgacat gaggcttcgc
     1501 atcgacgacc gctccaacca gcttgcaaca gcccttgcaa acaggctggc cgccgtgaag
     1561 gggcgttttt cgttgctgaa aaaccattta cgactcttga gtcccgcggc gacaatcagg
     1621 gagagacggt ttatagttga aaacttccac aagaatatgg tagccgcatg gtttctcttc
     1681 cgggagaggg tcgggaagcg gctcgctacc gacacggcgg ttctcgattc cttgagcccc
     1741 ttggccgttc tggggagggg atacggcatc gtgaggcgat tgccggaggg aacaatatta
     1801 agagagaccg gctctattgc ggcgggcgac gatgtgatgg taaaactata tgtaggtcgg
     1861 ttccaggcca gggtggtgaa aattgaagaa aggtaggaaa atggcgggag agaagtttga
     1921 agaagctctt gctaaactcg aggagatcgt aaaaaggatg gaaagtgggg aattgaccct
     1981 tgacgaatcc ctggaggcct ttgaagaagg cgtccggctt tcccggcttt gcgccgaaag
     2041 gctcgacgag gcggaaagac gcgtggaggc cctgctgaaa acgacagagg ggttcgaaat
//
LOCUS       DAOTMX010000303         2097 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_41191_length_2097_cov_4.015671, whole genome shotgun sequence.
ACCESSION   DAOTMX010000303 DAOTMX010000000
VERSION     DAOTMX010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41191_length_2097_cov_4.015671"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..893
                     /locus_tag="P5573_08010"
     CDS             111..893
                     /locus_tag="P5573_08010"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRT28044.1"
                     /translation="MIKAQTVDGLKIAYWMNDDGWVEGRKILFFVHGSGGDHTNWEYQ
                     YRDLHADFNILAVDLPGHGQSEGKGEQEVARYVEWVRKLIEALSLPKPVLIGHSLGAA
                     ISLTFAVRYGSLVSGIIPVGGGVKMPVNSLILDGIRKDTASVISLIVKFAVAKENRER
                     LAERLTKGLTAANPEVMYGDFLSCDRLDITEEISRIQVPTLLVCGEDDKMTPPALSQF
                     MEGRIPGAKLVLIPRAGHFVMMENPEAFNEAVRGFVEKDLTS"
     gene            902..1366
                     /locus_tag="P5573_08015"
     CDS             902..1366
                     /locus_tag="P5573_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRT28045.1"
                     /translation="MAKILIVYHTQTGNTDKMAQAVAEGARSIEDTEVVVKRASDATL
                     EDLLAADGLAVGTPENFGYMSGMVKDFFDRTFYPAQRQAERIFRKPYVVFVSAGNDGT
                     GALTSIERIALGYKFKKVYEPVISRKKLTGEDLERCRELGKILAGGCAAGIY"
     gene            1437..>2097
                     /locus_tag="P5573_08020"
     CDS             1437..>2097
                     /locus_tag="P5573_08020"
                     /inference="COORDINATES: protein motif:HMM:NF014589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HRT28046.1"
                     /translation="MGLSAFYHDSAAALLQNDEILAAAQEERFSRKKHDPGFPRNAVR
                     YCLKEANLSLKELDAVVFYDKPFLKFERLLETYYAFAPRGLASFLSAIPVWVKEKLML
                     KKLILDELCLIDAVDRKGVKLLFPEHHLSHAASAFYPSPFEESAILTVDGVGEWATAS
                     ICRGEGNRITILKELRFPHSLGLFYSAFTYYLGFRVNSGEYKLMGLAPYGDPNSERVL
                     KF"
BASE COUNT          483 a          570 c          617 g          427 t
ORIGIN      
        1 acggtgggga aggtcatgag aaaacagtta cgcgaggaag aactggcgaa gagaaaagag
       61 aaatctcaag ggtaggagat tcgagataga aaggatagga cggtattgcc atgattaaag
      121 cacagacggt cgacgggctg aagatcgcct actggatgaa cgacgacggc tgggttgaag
      181 gacgaaagat actctttttc gtccacggct ccggcggaga ccacactaac tgggaatatc
      241 aatacaggga ccttcacgcg gatttcaaca tcctcgccgt tgaccttccg ggccacgggc
      301 agtcggaagg gaaaggggag caggaagttg cccgctacgt cgagtgggta aggaaactca
      361 tagaagccct cagtttgcct aagcctgtgc tgatcggcca ttctctcggc gcggcgatca
      421 gtcttacctt cgccgttcgt tacggttctc tggtttcggg gatcataccg gtgggcggcg
      481 gcgtgaagat gcccgtcaac tcgctcatcc tcgacggaat aaggaaggac acggcctccg
      541 tcatttctct gatagtcaag ttcgccgtgg cgaaggaaaa ccgcgagcgc ctggccgagc
      601 ggctgacgaa gggcctcacc gccgcgaacc cggaagtcat gtacggcgac ttcctgtcct
      661 gcgatcggct ggacatcacg gaagagattt cccgtatcca ggtacccacg cttcttgtat
      721 gcggcgagga cgacaagatg acgccgccgg cactctctca gttcatggaa ggccgtattc
      781 ccggggcaaa gctcgtgctc atccccaggg cgggtcactt cgtgatgatg gaaaacccgg
      841 aggccttcaa cgaggccgtg agagggttcg tggaaaaaga cctgacctct tgagatgtac
      901 gatggcgaag atactgattg tttaccatac tcagacgggt aacacggata agatggcgca
      961 ggccgtagct gagggtgccc gcagcatcga ggatacggag gtcgtcgtca agcgggcgtc
     1021 tgacgcgacc ctggaggacc tcctggctgc cgacggcctt gccgtcggga cgccggaaaa
     1081 tttcggctac atgtccggca tggtgaagga tttttttgac cgcacctttt atcccgcaca
     1141 gcgacaggcc gaacgtatct ttcgcaagcc ttacgtcgtc ttcgtgagtg ccggcaacga
     1201 cggaacaggc gcgctcacga gcatagagag gatagccctc gggtacaaat tcaagaaggt
     1261 ctacgaaccg gtcatatcca ggaaaaagct gaccggtgaa gacctggaga gatgccggga
     1321 actgggcaag attctggcgg gcggttgcgc tgccggtatt tactgaaaag gtagaagacg
     1381 gacggaacat cgtaggcccg tcattcagag gagcaggacc ttattcggtg tacatattgg
     1441 gtttatcggc attctaccac gactccgccg cggcgctttt acagaacgac gagattctcg
     1501 cagcggcgca ggaagaacgc ttttcgcgaa aaaaacacga ccccggtttt ccccgcaacg
     1561 cggtccggta ctgtctgaaa gaggccaatc tttccctgaa ggaacttgac gcagtcgttt
     1621 tctacgacaa gccctttctt aagttcgaac gcctcctcga aacctactac gccttcgccc
     1681 cgaggggcct ggcatccttc ctttccgcga tccccgtctg ggtcaaagaa aagctgatgc
     1741 tgaaaaagct gatcctcgac gagctctgcc tgatcgacgc agtagacagg aagggcgtca
     1801 agctgctttt tcccgaacat catctttccc atgcggcgag cgccttctac ccctcgccct
     1861 tcgaagagtc ggcgatcctg accgtcgacg gcgttggaga gtgggcgacc gcttccatct
     1921 gccgggggga gggaaacagg atcacgatac tcaaggagct gagattcccc cattcgttgg
     1981 gcctcttcta ttcggccttt acctactatc tcggttttag ggtcaactcg ggcgagtata
     2041 aactcatggg acttgcccct tacggggacc cgaactccga acgggtcctc aagttcg
//
LOCUS       DAOTMX010000304         2089 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_41423_length_2089_cov_2.945428, whole genome shotgun sequence.
ACCESSION   DAOTMX010000304 DAOTMX010000000
VERSION     DAOTMX010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41423_length_2089_cov_2.945428"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            413..637
                     /locus_tag="P5573_08025"
     CDS             413..637
                     /locus_tag="P5573_08025"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRT28047.1"
                     /translation="MEESLGTIVQRHRKKSGLSRERLGEIAGVGKTVIYDIEHGKTTV
                     RFNTVKKVLDALNIRVRYISPLEESADEKG"
     gene            624..938
                     /locus_tag="P5573_08030"
     CDS             624..938
                     /locus_tag="P5573_08030"
                     /inference="COORDINATES: protein motif:HMM:TIGR03071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA N-terminal domain-containing protein"
                     /protein_id="HRT28048.1"
                     /translation="MRKADVYMQGKLAGVLQEIEKGKHYRFIYLDGYAGPPISLTMPV
                     RDRAFDFDRFPPFFDGLLPEGVLLEALLKRRKIDRRDYFSQIVAVGEDLVGAVTVKEA
                     QG"
     gene            935..1870
                     /locus_tag="P5573_08035"
     CDS             935..1870
                     /locus_tag="P5573_08035"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HRT28049.1"
                     /translation="MNRCPITYEDCGEERYSQRGLRLLSRRLTRLEDFPYSAAQQRRE
                     AIHRAGRMSIQGVQPKLSAVLNVEAGTFDIVESGGRYIIKPQMSEYPQVPENEDVTMK
                     LAEAAGLEVPFHGLIHSKDGTLSYVIRRFDRAGRSGKVPLEDFAQLLGQDRDTKYGSS
                     MEQVASVLDRYCTFPALEKLKLFKLVLFNYLTGNEDAHLKNFSLIRMDQKVELSPVYD
                     LLNTSIVIDSGEELALPLRGKKNKLKREDFTGYFAKERLGLKDKAISRTIRELEDAYP
                     LWINLLGRSFLSDKRKAMYRELLDRRMRTLELLHR"
     gene            1888..>2089
                     /locus_tag="P5573_08040"
     CDS             1888..>2089
                     /locus_tag="P5573_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT28050.1"
                     /translation="MRTGRDFNASDRIKENPHSAAFPEWGFLFEDIVVLRAFFSPAGP
                     RALRRSADQVLHDLGHGVREQGH"
BASE COUNT          470 a          581 c          608 g          430 t
ORIGIN      
        1 atcccccgcc ccctgggggc gtgacggttc gattccgtcc tccggcacca tgaaaatcaa
       61 ggggttgtgg cccgaaaggc gcaatccctt ttttcattca tcgtaccgca ggcgatcctg
      121 tcgtgcggtt gccgcgatct ttccaaagac actcatcagg tctatacggc tcggcgatga
      181 tatccccgac gtttttcgcc gggaatagct gcagggagct gattttcaaa taatctaccg
      241 acatagcggt aaaagttgat gaattattgc ggcaggatgc attcaaaagt ggaaatcacc
      301 tgcatattgc cgttataacg gtaaatataa cttgacataa tgcattatag ggcttaatga
      361 agcgtaggag tgccaatact gccgtcatcg cggcaaatgg aaggaaatac acatggagga
      421 atccctgggt accattgtgc agcggcaccg aaagaagagc ggcctgtcgc gggagcgact
      481 tggcgaaatc gccggcgtcg ggaagaccgt aatttacgac atcgaacacg gcaagacgac
      541 agtccgattc aacacggtga aaaaggtcct tgatgcgctg aacattcgag tccgctatat
      601 cagtccgctc gaggagagtg ccgatgagaa aggctgatgt ctacatgcag ggaaagcttg
      661 ccggcgttct tcaagagatc gaaaagggaa agcattaccg ctttatctac ctggacggct
      721 acgccggccc gcctatctcg ctcaccatgc ctgtccggga tcgggccttc gacttcgacc
      781 gattcccgcc gttcttcgac ggcctgctgc cggaaggcgt tctcctggag gccctcctga
      841 agcgccgcaa gatcgaccgc cgtgattact tcagccagat cgtggccgtg ggggaggacc
      901 ttgtgggtgc cgtcaccgtg aaggaggcgc agggatgaac cgctgtccca tcacctatga
      961 agactgcggc gaggagagat attcccagcg cggattgcgc ctgctctcgc gacggctgac
     1021 ccgtctggaa gactttccct acagcgccgc gcagcagcgg cgggaggcga tccatcgggc
     1081 aggcaggatg tccatccagg gcgttcagcc gaaactcagc gccgttctca atgtcgaggc
     1141 gggcacattt gacattgtag agtccggggg acgctacatc atcaagcccc agatgtccga
     1201 gtaccctcaa gtccctgaaa atgaggacgt gacgatgaag ctggcggagg cggcggggct
     1261 ggaggttcca ttccacggtt tgatccattc aaaggacggc accctttctt acgtcatccg
     1321 gcgctttgac cgcgccggac gctcgggaaa ggttcccctc gaggacttcg cccaactcct
     1381 gggacaggat cgggatacca agtacgggtc ttccatggaa caggtcgcgt cggtcctcga
     1441 ccggtactgc acgttcccgg ccctggagaa gctcaagctg ttcaagctcg tgctgttcaa
     1501 ctatctcacg gggaacgagg acgctcacct gaagaatttc tcactcatcc gcatggacca
     1561 gaaggtagaa ctgtcgccgg tctacgacct tctcaacacg tcgatcgtca tcgattcggg
     1621 ggaagagctg gctctgccgc tcagaggcaa gaaaaacaag ctgaagcggg aggatttcac
     1681 ggggtacttc gcgaaggaga gactcggtct caaggacaag gcgatctcca ggacgatcag
     1741 agaactcgaa gacgcctacc cactctggat aaatctcctt ggcaggagct tcctttccga
     1801 taaacggaaa gcgatgtatc gggagttgct cgatcggcgg atgagaacgc tggagttgct
     1861 gcatcgctga gtcgacggga aacccttgtg cgaacgggaa gggatttcaa cgcgtccgac
     1921 cggataaagg aaaaccccca ttccgccgcc tttccggaat gggggttttt atttgaggac
     1981 atcgttgtct tgcgcgcttt tttctctccc gcaggccccc gggcgctgcg tcgatcagcc
     2041 gatcaggttc ttcacgacct cggccacggg gttcgtgaac agggccacc
//
LOCUS       DAOTMX010000305         2086 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_41503_length_2086_cov_4.640079, whole genome shotgun sequence.
ACCESSION   DAOTMX010000305 DAOTMX010000000
VERSION     DAOTMX010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41503_length_2086_cov_4.640079"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            264..1697
                     /locus_tag="P5573_08045"
     CDS             264..1697
                     /locus_tag="P5573_08045"
                     /inference="COORDINATES: protein motif:HMM:NF023222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HRT28051.1"
                     /translation="MALRSKEEYIESLKQKRPRVYIGGEKVDRVWESEYFKTSINQIG
                     ATYEFALDPQYREWSAVKSPLTDEEVPRNSLHIQQSLEDAVIKAHLTREVTKRRICTY
                     CLSNFLSVTWTFTHDIDGKYGTKYHQRFKEYVKHLQKNDLRLAWGMMDPKGDRRFGPS
                     KQDPPVDLRILERTEDGIVVSGAKVHTTYGPVAHEVIAVPCRALSEDDRDFAVSFAVP
                     VDTEGVTFICRPAPGPRRPVNMESPLSSKFVGVEAMTIFDRVFVHWERVFMCGEWDMC
                     GNLPTYFSSLHRQSKCACSAGHADLMAGTCSLLAKVNGLSLKVSHINDKITDMMMAAE
                     VAFGCSLGAALNGRMHPSGVWMPSALIANAGLHHIRSSLGTHISHIHDIAGGLLTTMP
                     VEADIDNPETKKMIDLYLRGSKDYTTEQRLRILELGRDLAASNLCGAIMGFTINAAGS
                     PVTNKIAVSRNYNLKEMEDIAKEIAGI"
     gene            complement(1809..>2086)
                     /locus_tag="P5573_08050"
     CDS             complement(1809..>2086)
                     /locus_tag="P5573_08050"
                     /inference="COORDINATES: protein motif:HMM:NF017652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0019475 - L-lysine catabolic process to
                     acetate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HRT28052.1"
                     /translation="KRLIGEGNFEFSVCVAGRAQFPICTQSLLIGGNVRVGLEDNLYL
                     EKGVMAKSNAEQVAKIVRIAKEFGIEPATPDEARKILGLKGMDKINL"
BASE COUNT          497 a          579 c          564 g          446 t
ORIGIN      
        1 tctaataaca aataattatg cgtgatttta cataagattt taacacaata ccatgttttt
       61 tatgaacgcc aaccgacaca tgtgatatgc ttttctagga tttgcttttt tgctatgact
      121 ctgcactgga ctattccagg accatcagca ttacctacgc cgcagagggc aggtgagctt
      181 tttggccgtt aacagttatg tgcaggggga ggttgaccct tgagtcagga aagctgaaac
      241 tgattcggaa ggaggttggt cgaatggcac tacgttcaaa agaggaatac atcgaaagcc
      301 tcaaacaaaa aaggccgcgg gtctatatcg gcggtgagaa agtggaccgt gtctgggaga
      361 gtgagtattt taagacatcc atcaaccaga tcggcgccac atatgaattc gccctggacc
      421 cgcaatatcg ggaatggtcc gccgtcaaat ctccactcac agacgaagaa gttccgagga
      481 actctctcca tattcagcaa tccttggagg acgccgtcat caaggcccac ctgacacgag
      541 aggtgacgaa aaggcgtatc tgtacttatt gcctgtccaa ttttctctcc gtcacctgga
      601 ccttcaccca cgacatcgac gggaaatacg gcaccaagta tcatcaaaga ttcaaggaat
      661 atgtgaaaca tctgcaaaaa aacgacctcc gtctcgcctg ggggatgatg gatcccaagg
      721 gagacaggcg cttcgggccg tccaagcagg acccgccggt cgacctgcgg atattggaga
      781 gaacggagga cggcatagtc gtctccgggg cgaaagtcca tacgacctac ggcccggtgg
      841 cccacgaggt catcgccgta ccctgcaggg ccctctccga agacgataga gactttgccg
      901 tttcttttgc cgtccccgtc gacaccgagg gtgtcacctt catctgcagg cccgcccccg
      961 gccccaggcg tcccgtgaac atggaaagtc ctctgtcgtc gaagttcgtc ggggtcgagg
     1021 cgatgactat cttcgaccgt gtcttcgtcc actgggaacg ggtcttcatg tgcggtgaat
     1081 gggatatgtg cgggaatcta ccgacttatt tttcatcctt gcaccggcag tccaagtgcg
     1141 cctgttccgc cgggcacgcc gatttgatgg ccgggacttg ttcactgctg gcgaaagtca
     1201 acggattgag tctcaaggtg agccatatca atgacaaaat caccgacatg atgatggcgg
     1261 cagaagttgc ctttgggtgt tctttggggg ctgccttgaa cggcagaatg catccgagcg
     1321 gtgtctggat gccgagcgcg ttgatcgcga acgcggggtt acaccacatc aggtcatcgc
     1381 tgggaacgca catcagccat atacacgaca tcgccggagg cctcctcacg acaatgccgg
     1441 tcgaggccga tatcgacaac ccggaaacaa aaaaaatgat cgacttatac ctgagaggca
     1501 gcaaggacta cactacagaa cagaggctgc gaatcctcga gctgggtaga gacctggcgg
     1561 cctccaatct gtgcggggcc atcatggggt tcacgatcaa tgccgccggt tcccccgtga
     1621 cgaataagat cgcggtctca cgaaactata acctgaaaga gatggaggac atcgcgaaag
     1681 agatagccgg tatttaaccc ctccgacgga gaaaggcacc acacgacggc ccgcccgctg
     1741 ggtgccttct ccgatggaca tcatgcgcgg aaggatcgga cgccctgctg cccggcggcg
     1801 gtgtccggtc acagattaat tttgtccata cctttcaaac caagtatctt cctggcctcg
     1861 tctggtgttg ccggctctat gccgaactct ttcgcgatcc tcacgatctt ggccacctgc
     1921 tcggcattgc tcttcgccat gacccccttt tcgagataaa ggttatcctc caggccgacg
     1981 cggacgttgc cgccgatgag aagggactgg gtgcagatcg gaaactgggc acgccccgcc
     2041 acgcagacgg agaactcgaa gttcccctcc ccgatcagcc gcttcg
//
LOCUS       DAOTMX010000306         2074 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_41837_length_2074_cov_3.694403, whole genome shotgun sequence.
ACCESSION   DAOTMX010000306 DAOTMX010000000
VERSION     DAOTMX010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41837_length_2074_cov_3.694403"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            203..427
                     /locus_tag="P5573_08055"
     CDS             203..427
                     /locus_tag="P5573_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT28053.1"
                     /translation="MKSADTRQQLKAVYETMVIADSVAMEVHLGKNMPGKMVGCINSE
                     KMPEVMPDPGPRVCSRGDFRVSRRLTDASG"
     gene            complement(408..851)
                     /locus_tag="P5573_08060"
     CDS             complement(408..851)
                     /locus_tag="P5573_08060"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HRT28054.1"
                     /translation="MILYFEDFEVGHRFRSPGITVTESQVIDFAMHYDPQVFHIDTEA
                     AKATTYGGLIASGIHTIALTFRLFLMTGALEGSSLGSPGVDDLRWLRPVRPGDTLHVV
                     GEVLEKKASSSRQDRGIVRIRYTSYNQRDEEVMTMVGNQIIRRRP"
     gene            complement(926..1672)
                     /locus_tag="P5573_08065"
     CDS             complement(926..1672)
                     /locus_tag="P5573_08065"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HRT28055.1"
                     /translation="MIGYIERHTRIQGEGILPRDVLVWLPPGYGSPPGQRYPVLYMQD
                     GQNIFEEGNSLSGLSWRTDETAGRLMLEGRIRKAIIVALYNTEDRQEEYGDTEKGRAY
                     MRFVVEKVKTVIDGAYRTVSGRHGTFVMGSSMGALISFLLVWNHPEVFSAAACLSPAF
                     VYGGNNVIPLVQEYRGPRKEIRLYFDNGTVGLDRELQEGCDEMIEALQEKGFVLGRDV
                     EWYLDEGAEHSEAAWSRRLWRPLRFLLGKT"
BASE COUNT          467 a          592 c          538 g          477 t
ORIGIN      
        1 ggcgaaggaa acaccgagtc ttgccgccaa ttcctcctgg ctgaggttaa gccttcccct
       61 caactctttg acgaccctac ctatatcttt actctcgagc ataacagccc tccggttatt
      121 gaagattatg ctatagcatt atgacgttct atgtcaacgg tttttattcg caaggaggcg
      181 caaatttgta tgtgacgatg tggtgaaatc agcagatacc agacaacagt tgaaggctgt
      241 ctatgagacg atggtcatag cagactcagt ggcgatggag gttcatctcg ggaaaaatat
      301 gcccggtaag atggtgggtt gtatcaatag cgaaaagatg ccggaagtca tgcctgaccc
      361 cggtccccgg gtgtgttccc gcggcgattt cagggtttcc cgaaggctta cggacgcctc
      421 cggatgatct gatttccgac catcgtcata acctcctcgt cccgctggtt gtaactcgtg
      481 tagcgtatac ggacgatccc ccggtcctgc cgcgagctgg aggccttttt ttcaagcacc
      541 tcaccgacga cgtgaagcgt gtcgccgggc cggacgggac gcagccaccg caaatcatct
      601 actccgggag acccgaggct ggagccttcc agcgcccccg tcatgaggaa aagccgaaag
      661 gtcagagcaa tggtgtgaat cccgctcgcg atgagtcctc cgtaagtggt tgcctttgcc
      721 gcctcggtgt cgatgtgaaa gacctgcggg tcgtaatgca ttgcgaaatc gatgacctgg
      781 ctctccgtaa ccgtgatccc cggcgaccgg aatcgatggc ccacttcaaa atcttcaaaa
      841 tagagaatca ccgttcaacc cccgaggaag gtcaattgct cagtttgcgg cacagcttat
      901 atacaaccgg cggaaaaagg caagactaag tttttccaag cagaaaacga agaggccgcc
      961 aaaggcgtct cgaccacgcc gcctccgagt gttctgcccc ctcatcgagg taccactcca
     1021 catcccgtcc caggacaaac cccttttctt ggagcgcctc tatcatctca tcgcaccctt
     1081 cctgcagttc ccggtccagg ccgacggtgc cgttatcgaa gtaaagacgt atttctttgc
     1141 ggggtccccg atactcttga accagcggga tgacgttgtt gccgccgtat acaaaggccg
     1201 gtgagaggca ggctgccgcg gagaagacct ccgggtgatt ccagaccagc agaaaagaaa
     1261 tcagcgcccc catggaagac cccatcacga aggtcccgtg tcttccggaa acagtccggt
     1321 aggcgccgtc gataacggtt ttgaccttct caacaacaaa ccgcatgtaa gcccttccct
     1381 tttcggtgtc tccatactcc tcctgccggt cttccgtatt gtacagggcg acgatgatgg
     1441 ccttcctgat ccgcccttcc agcatcagcc ggcccgccgt ctcgtcggtc cgccaggaga
     1501 ggccgctcaa tgaattcccc tcctcgaaaa tgttctggcc atcctgcatg tacaggacgg
     1561 ggtaacgctg ccccggggga gatccgtagc ccggcgggag ccagacaagg acgtccctgg
     1621 ggagtatccc ctcgccctgt atccgggtat ggcgttcgat gtagccgatc atcggggctc
     1681 agaaatatgg gttgctactc gcggcatagt cggccggtct tatatcagtc cacccagtga
     1741 tggaaacgac ggcacaccgg aagcgcatga gcaaatatct ctttatgcac tgtgccgtaa
     1801 tcacccagaa ctcgacgaca gaagttgtca aggcccgctg gagataaatc catatcgtgg
     1861 gctattataa cccattttca tccttcgtgg atgccgctac agcatgaaaa ttttgttgca
     1921 tttcgatgga cattatgctc cattttaaaa gatatttctg ttgatagacg gagacctttg
     1981 aatttgtgat taaacggcct tgtcgtcatt ccgtccgtgc ttgacttaag cctgccccgt
     2041 actttatacg ggggaatcca gtgttttcag gagg
//
LOCUS       DAOTMX010000307         2035 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_42923_length_2035_cov_4.226768, whole genome shotgun sequence.
ACCESSION   DAOTMX010000307 DAOTMX010000000
VERSION     DAOTMX010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42923_length_2035_cov_4.226768"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="P5573_08070"
     CDS             complement(<1..538)
                     /locus_tag="P5573_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT28056.1"
                     /translation="MEYYGAEKDKTKEKPYRPSLNGRKKAVVAVVVAAVLTLAAILSF
                     YYAGEKKLDRLRDTALSIIPGNKPRFYSLLIEKNGKDYELAEGDTFEVTYRDVFVIKK
                     VSTSSPFGLWLSADIEGLGTENDLGVLLKGVDLVDKVVSVENAVGAPAGRGLIKVRYR
                     GEVIADIPVNVVITPQDWV"
     gene            complement(541..1428)
                     /gene="xerD"
                     /locus_tag="P5573_08075"
     CDS             complement(541..1428)
                     /gene="xerD"
                     /locus_tag="P5573_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HRT28057.1"
                     /translation="MNLLLDEYMKHLAMEKGLSLNTLEAYSRDIGRFLAFMEAEGLKR
                     IQDISSDHVITYLGMQRRQGLSSASVNRGMAAIRGFYRYLVAEKKVDDNPVAHIDSSK
                     AWMRLPDTLTKEEMTRLIEAPSGKTPSDLRDTAMLELLYATGLRVSELIDLTTNNVNW
                     QAGYLIARGKGNKERVVPIGRSALVALNLYMEKGRPEFLKGKENGILFLNRFGRGLTR
                     QGIWKIVRKYAAQVGLAGKVHPHTFRHSFATHLLEGGADLRSVQAMLGHADIATTQIY
                     THVTRERLREVHRKFHPRG"
     gene            1584..>2035
                     /locus_tag="P5573_08080"
     CDS             1584..>2035
                     /locus_tag="P5573_08080"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRT28058.1"
                     /translation="MKAKNIILALAGLAVAAALVVFILPKKEADIVRIGVFLPMTGGV
                     ASYGQMGWAGIKTANELEPTVLGKKVELILVDEKSDKIEAANAVSRLIKKEKVVALIG
                     TSTSGNTMAGAAIAEEARVPVVSPTATDPRVTQNRKYIFRVCFIDPFQG"
BASE COUNT          459 a          590 c          488 g          498 t
ORIGIN      
        1 ttacccagtc ctgtggtgtt atgacgacat tgaccggtat gtcggcgata acttcgcccc
       61 ggtaccgaac cttgataagt ccgcggccgg caggcgcccc caccgcattt tccacagata
      121 ctaccttgtc gaccaggtct acacccttca acagcacacc taggtcgttc tcagtgccca
      181 ggccttcgat atccgcagac aaccacagac caaaaggaga gctcgtcgat accttcttaa
      241 tcacaaatac atcgcggtaa gttacttcga atgtgtcccc ttcggccagc tcatagtcct
      301 tgccgttttt ctcgatcaga agggaataaa acctcggctt gttcccagga atgatcgata
      361 aggcggtatc cctcaatcgg tcaagtttct tctctccggc gtagtagaaa gagagtattg
      421 ccgccagggt aagcaccgct gcgaccacga ccgccacgac cgcttttttc cttccgttaa
      481 gcgacggcct gtacggtttt tctttcgtct tgtccttttc agccccgtag tattccaatg
      541 ctaccccctg ggatggaatt tcctgtggac ttccctcagc ctctcccttg tgacgtgcgt
      601 gtagatctga gtggtagcta tatcagcgtg accgagcatc gcctgaaccg acctgaggtc
      661 ggcgcccccc tcgaggagat gtgtcgcaaa agaatgcctg aacgtatgcg gatgaacctt
      721 gccggcaaga ccgacctgtg cggcgtattt cctgacgatc ttccatatcc cctggcgtgt
      781 caggcctcgt ccgaaacgat tcaggaaaag aatgccgttt tcttttccct tgagaaattc
      841 cggccgaccc ttctccatgt ataggttcaa ggccacgagc gcagaccgcc ctatcggtac
      901 gacccgctcc ttatttccct tgccgcgggc gatcaggtat cccgcctgcc agttgacgtt
      961 gttagtcgtg aggtcgatca gttccgacac cctcaatccc gtcgcataca acaactccag
     1021 catggccgta tctctcaagt ccgaaggagt tttccctgac ggcgcctcta tgaggcgggt
     1081 catttcttcc ttcgtcagcg tgtccgggag tctcatccat gcttttgagg agtcgatgtg
     1141 ggcgaccggg ttatcatcga ctttcttttc tgcgaccagg taccggtaaa atcccctgat
     1201 agcggccata cccctgttta cggacgcgga tgaaagccct tgcctacgtt gcatccctaa
     1261 atatgttatc acatgatcgg acgaaatgtc ttgaatcctc ttcaacccct ccgcttccat
     1321 gaaagcgaga aaccgcccga tgtcgcggct gtaagcttcc agcgtgttca gggagaggcc
     1381 cttttccata gcgagatgtt tcatgtattc gtcgagcaat agattcatca ccctgccgtt
     1441 ctttctccga aatggtgcag atgatgtata tcatgaatca tccattgaca tatcctcctt
     1501 ttcgtgtttt ataaaaaaat tcgttcgcgg ccttaaattt tcccgctttt aattcacggc
     1561 tccttaaaga aaagagaggc atcatgaagg cgaaaaacat tattttggcg ttagccgggc
     1621 ttgccgttgc cgccgcactc gttgttttca tcttaccaaa aaaagaggcc gacatcgtca
     1681 gaataggcgt ctttcttccc atgaccggag gtgtcgcttc ctacggacag atgggatggg
     1741 cgggtatcaa aacggccaat gagcttgaac cgacggtcct ggggaaaaaa gtcgagctca
     1801 tccttgtaga cgagaagagc gacaaaatcg aagcagccaa cgcagtatca cggctcatca
     1861 agaaagaaaa ggtcgtcgcc ctcatcggca catccacaag cggcaacacg atggccggtg
     1921 cggcgatagc cgaggaggcc agggtccccg tcgtcagccc aacggcaacg gacccacggg
     1981 ttacacagaa ccgaaaatac atattccggg tctgcttcat cgaccccttc caggg
//
LOCUS       DAOTMX010000308         1999 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_43999_length_1999_cov_3.354424, whole genome shotgun sequence.
ACCESSION   DAOTMX010000308 DAOTMX010000000
VERSION     DAOTMX010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43999_length_1999_cov_3.354424"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /locus_tag="P5573_08085"
     CDS             complement(<1..715)
                     /locus_tag="P5573_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HRT28059.1"
                     /translation="MFYADRCDLCGKCLEFCPYVDYDLARAQKEMKELVEGGTPPIVG
                     LCVTCASCNLICPTQANPFDLLNERQEKTGALGIPDQAMQNFAKLHSLPTVVKKGKEG
                     APVLNLCIVGDMVRELLDNPLFEGLTVVEGADYFCTVGYIHLGQPSPVKKEAKRFIDN
                     LAALGAKEVICFHDDCYTLLTTLAQEYGLKVPFRPIHIFEYLYRELLERKDRIKKIDI
                     KVAYQSPCASRYTQGKDVYL"
     gene            complement(988..1302)
                     /locus_tag="P5573_08090"
     CDS             complement(988..1302)
                     /locus_tag="P5573_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT28060.1"
                     /translation="MRIGIASYKGSILSGILAFGAWGFVIVAGSFLSFWLGLMIDKQL
                     RTEPYFMLGLFFLAIMFIVGRLWRKAMEMTDRLSGGIIPENERRALRDLTKRGITFGY
                     RR"
     gene            complement(1352..1627)
                     /locus_tag="P5573_08095"
     CDS             complement(1352..1627)
                     /locus_tag="P5573_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT28061.1"
                     /translation="MKKTLSTLILTAIVVLITATFSFAEYAAAGADNFPYFHLGALIV
                     GGLTIISLKQKYQKLYLSEAAGSFAMYAVLVALFTSPVTEAIRNLIG"
     gene            complement(1797..>1999)
                     /locus_tag="P5573_08100"
     CDS             complement(1797..>1999)
                     /locus_tag="P5573_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT28062.1"
                     /translation="RPIKAVKRNAPFFDVPYPDRSETKQVFSKCLTEKTDRQAPVRGR
                     CLPGPGLSHFFNRFVHPVERFF"
BASE COUNT          439 a          513 c          592 g          455 t
ORIGIN      
        1 cgaggtagac gtccttgccc tgcgtgtatc gtgacgcaca gggggactga taggcgacct
       61 tgatgtcgat cttcttgatc cggtcctttc tctccagaag ttccctgtag agatactcga
      121 agatgtggat cggccggaag gggaccttca ggccgtattc ctgcgccagg gtggtcaaca
      181 gggtgtaaca gtcgtcgtga aagcagataa cctccttagc tccgagagcg gcgaggttgt
      241 cgatgaagcg ctttgcctct ttcttgacgg gactcggttg accgagatgg atgtacccca
      301 cggtgcagaa atagtcggcg ccttcaacaa ctgtcagacc ctcgaaaagc gggttgtcca
      361 ggagttcccg gaccatgtcc ccgacgatgc agagattgag caccggcgca ccctccttgc
      421 ccttcttgac aaccgtcggc agactgtgga gcttggcgaa gttctgcatg gcctgatcgg
      481 ggattcccag ggcgcccgtt ttttcctggc gttcgttgag caggtcgaag gggttggcct
      541 gtgtggggca gatcaggttg caggacgcgc aggtcacgca gaggcccacg atgggcggtg
      601 tgcccccctc gacaagctct ttcatttcct tctgggcccg cgcaagatcg tagtcgacat
      661 agggacagaa ctcgaggcat ttaccgcaga gatcgcatct atcggcgtaa aacatggata
      721 tcctccttcg cttttttagt gggatcgggc atgccggcgc gtactctaac acatcctgcg
      781 atgatttcga attaaaatct gcggcccgat ataaaggatg atgagaccct tcgacatcgc
      841 ccggcccggg ggtgttgatc ggtaaggatc ccgtgtccgg cacacagggt gcttacaaag
      901 gtgagagcga ccgcgagaaa caaagaaaag gcaggaccgg aaacagccct gcctcagtat
      961 cattcaaacg gggaggttgt cgcgcctcta ccgccggtat ccgaaggtga tccccctctt
     1021 ggtcaggtct ctgagggctc ttctctcgtt ctcggggatg atgccgccac tcagcctgtc
     1081 cgtcatctcc atggccttcc tccacagtct gccgacgatg aacatgatgg cgaggaagaa
     1141 gagcccgagc atgaagtaag gttcggtccg gagttgctta tcgatcatga gccccagcca
     1201 gaaggagagg aaagagccgg caacgatcac aaacccccag gcgccgaagg ccagaatgcc
     1261 ggagaggatg ctacctttgt atgatgcgat tccgattctc atgggatgct cccggagccg
     1321 gtcatagccg gctccctgtc tgcatgttcg gttagccgat gagattcctg attgcctcgg
     1381 tcacggggct cgtgaagagc gccaccagca ccgcgtacat tgcgaagctg cccgcggctt
     1441 cactgaggta gagtttctgg tacttctgct tgagggaaat gatcgtcagg ccgccgacaa
     1501 tcagggctcc caggtggaaa taggggaagt tgtcggcgcc ggcagccgca tactcggcga
     1561 agctgaaggt tgcagtgatc agaacgacta tcgcggtgag aatcagggtg gaaagtgtct
     1621 ttttcatttc gaggtcctcc tcggtttttt ttgttatttg ccctgttata tagaaaggag
     1681 cgtgccagaa tgaggcatga cgacaagata atttacaagt tattgatatg aatgagaaat
     1741 attgcatgct tcagccggaa tagttcgcct gcgacggcat ccggaagcct ttatttttaa
     1801 aagaaacgtt caaccgggtg aacgaaacga ttaaaaaaat gagagaggcc ggggccgggg
     1861 aggcagcggc ctcgcaccgg cgcttgccgg tcagtctttt ccgtcaggca ttttgaaaat
     1921 acttgttttg tttcagatcg gtcggggtaa gggacatcaa aaaaaggtgc atttcttttg
     1981 acggctttga tcggcctgc
//
LOCUS       DAOTMX010000309         1991 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_44237_length_1991_cov_3.609504, whole genome shotgun sequence.
ACCESSION   DAOTMX010000309 DAOTMX010000000
VERSION     DAOTMX010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44237_length_1991_cov_3.609504"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..758
                     /locus_tag="P5573_08105"
     CDS             111..758
                     /locus_tag="P5573_08105"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MtnX-like HAD-IB family phosphatase"
                     /protein_id="HRT28063.1"
                     /translation="MTGRTIVLSDFDGTISAQDIGHEVIKRFMNGDWRELDDSYASGK
                     IGSMAAYRRIASLLKAEPREMAEYALSLNTMDPTFAGFYRFCKQRGFDFKVVSDGLDF
                     YIRLILERHGLGEIEFFSNVLRFNGGFSVSIEFPCSNDECGRCGTCKSMILQNERSEY
                     ERIIYIGDGYSDVCPTQYADMVFGKGILYEKCRQNNRACFHYRNFQDIVDILDRQ"
     gene            764..1717
                     /gene="radC"
                     /locus_tag="P5573_08110"
     CDS             764..1717
                     /gene="radC"
                     /locus_tag="P5573_08110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HRT28064.1"
                     /translation="MKSRENTAGEPPGCRTEEETIKQLAEKALFPVMSRRGKVYLHPA
                     GRVRPTAEESRKIIEDLKIEDADRCRELRKRIGELRAASRESRPIRTWVRSERPRERL
                     QRNGPETLTDSALLAIIVRTGMEGVDAEELGRRLINRFGSFRALDDAPISEICSISGI
                     GPAKAVQVKAAIEIGKRLLRERAGKKRRVRNPSDIVDYVAEYYGPYLRDSRKEVFSVI
                     FTDIKNKPLHHVEVSRGSVSASIVDPGEIIREASLRSASAVILIHNHPSGETEPSTED
                     IRLTRRIVEACSLLNIKVLDHIIIGKNLEDYYSFSAAGLIG"
     gene            complement(1790..>1991)
                     /locus_tag="P5573_08115"
     CDS             complement(1790..>1991)
                     /locus_tag="P5573_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT28065.1"
                     /translation="LQGLYQWRVGGQDEAAIDKRHGIYYDTQTGTLAAVNFFKEKGVR
                     VISVNGLPGVQEVSEELLKKLQ"
BASE COUNT          478 a          530 c          573 g          410 t
ORIGIN      
        1 ttcctgctgt tcgagaattg gtacgtcaaa atcagtgatt tgtcgacgta tgtgttgccc
       61 gtcctcttga tcgctcttgg ggcctacata ttgttcggaa ggtcgaagaa atgaccggca
      121 gaaccatcgt tctgagcgat tttgacggta caattagcgc acaagacatc ggccatgagg
      181 tgatcaagcg cttcatgaat ggtgactgga gggagctcga cgactcgtat gcatccggca
      241 agatcggttc gatggcggca taccgaagga tcgcctcgct cctgaaggcc gagccgcggg
      301 agatggccga gtatgcccta agcctgaaca ccatggaccc aacctttgcc ggtttttacc
      361 ggttctgcaa gcaaaggggg ttcgacttca aggtcgtgtc cgacggtctc gacttttaca
      421 tccgcttgat cctggagcgc cacggcctcg gggagataga gttcttttcg aacgtcctgc
      481 gtttcaacgg aggtttttcg gtctcgatcg aatttccctg ctccaacgac gaatgcggcc
      541 ggtgcggaac ctgcaagagt atgatcctgc agaatgagcg ctccgaatac gaaaggatca
      601 tctatatagg cgacgggtac tccgatgtct gtccgacgca gtacgccgat atggttttcg
      661 gtaagggaat cctctacgaa aaatgccggc agaacaacag ggcgtgcttc cactacagga
      721 attttcagga catcgtcgat atccttgacc ggcagtaagt gaaatgaaaa gtcgagaaaa
      781 cacggccgga gaaccccctg gctgccggac cgaagaagaa acaatcaagc aactcgccga
      841 aaaggccctc ttccccgtga tgagtcggcg ggggaaggtg tatctgcatc ctgccgggag
      901 ggttcgccct acggccgaag agtcgcgaaa gatcatcgaa gacctgaaga tcgaagacgc
      961 cgacaggtgc cgggaattaa ggaagaggat aggggaattg agggctgctt cccgggagtc
     1021 aaggccgata cgcacttggg tcaggagtga aaggccgaga gaaaggttgc agcggaacgg
     1081 gcctgagaca ctgacggatt ccgctcttct tgccatcatc gtgaggacgg gtatggaggg
     1141 cgtcgatgcg gaagaactgg gcagacgatt gataaaccgc ttcggttcat tcagggcact
     1201 cgacgacgcc cctatctccg agatatgcag cataagtggc ataggccctg ctaaagccgt
     1261 acaggtgaag gcggccatcg aaatcggaaa acgcctcttg cgggaaaggg cgggaaagaa
     1321 acggagggtc aggaacccgt ccgacatcgt ggattacgtg gccgagtact acggcccata
     1381 cctgagggac tcgcgcaaag aggtcttcag tgtcattttt acggacatca agaacaagcc
     1441 cctccaccac gtagaggtca gcagggggag cgtcagcgcc agcatcgtag accccggcga
     1501 gatcatacgc gaggcctcgc ttcgttccgc ctcagccgtg atcctcatcc acaatcatcc
     1561 ttccggcgag acggagccgt ctaccgaaga catccgcttg acacgacgca tagtcgaggc
     1621 ctgcagcctc ctgaacatca aggtactgga ccacataatc atcggaaaga acctggaaga
     1681 ttattacagc ttttccgccg ccgggttgat cggataagtt cgcaatctac ccgcaaaaaa
     1741 ggcccttcca ccgaagagga ggggcctttt tgtgtaagaa acagtcagcc tactgcagct
     1801 tcttcaacag ttcttccgag acctcttgca cgccgggaag gccgttcacg ctgatcaccc
     1861 ggactccctt ttctttgaag aaatttactg cggcgagggt tcccgtctgt gtgtcatagt
     1921 aaataccgtg ccgcttatcg atggcggctt cgtcctggcc gcctacgcgc cattggtaga
     1981 gcccctgcag g
//
LOCUS       DAOTMX010000310         1989 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_44302_length_1989_cov_3.544467, whole genome shotgun sequence.
ACCESSION   DAOTMX010000310 DAOTMX010000000
VERSION     DAOTMX010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44302_length_1989_cov_3.544467"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="P5573_08120"
     CDS             <1..602
                     /locus_tag="P5573_08120"
                     /inference="COORDINATES: protein motif:HMM:NF013715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HRT28066.1"
                     /translation="KFGKLDTFFGSLMTCLVAAFIIITTGAVFYYHTPPILIEDARQT
                     AEAIVPLMPAGEGELARKLFAIGLFDAGFLGALCISLATSWSFGEVFGWAHSLNQRVR
                     EAPWFYIVYLGILATSGAVVLIPEAPLVTITMFVQVIAVTLLPASLVFMIILLNDRSF
                     MGDHVNTGWQNFVNGSIILVVIVMSTLMGLNTLFPNLFG"
     gene            621..1919
                     /locus_tag="P5573_08125"
     CDS             621..1919
                     /locus_tag="P5573_08125"
                     /inference="COORDINATES: protein motif:HMM:NF015413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRT28067.1"
                     /translation="MADQTNSKAKVADIPRSIGSYRFLNFSELIKRPVCVGNIKNRVG
                     RLTDLVFALAEPYPEVVGIYIEHGWGKPTEFIPWSKVVKIEDDAIFVHPPDQGDSFPP
                     FVDQPGWILLDKHLMGRTILDIDGRRSEVVNDVHLLEAKGRLLLVHVDVSMSGFFRRL
                     GLGKLRWVKDNLISWKYVQPLSVEDAIATDKVTLSITRKQLLELPSEDLADVLEELPG
                     EEQQALFSALDSEKAAETLVEAEPRAQRRIIASLRADRARNILSEMTVPQLVGLFSVL
                     PHDDVEKLMGLLSNEKAERVRDILSEREVQAKDIMLPDFVTVFKTDKVGEILEKIRRS
                     GREPRSISYVYVVDSDGLTLLGVVDLRELVLAADHVEAGDIMASPVVTANEDDLQEDI
                     AAMFAKYHYEMIPVVDDQDRIMGVILYSDVIKSIESRVKV"
BASE COUNT          408 a          555 c          584 g          442 t
ORIGIN      
        1 taaaattcgg caaactggat acttttttcg ggtctctgat gacctgcctg gtcgctgcct
       61 ttatcatcat cacgacgggc gccgtgttct actatcacac gcccccgatc ttgatcgagg
      121 acgcaagaca gacggccgaa gcgatcgtgc cgctcatgcc ggcgggggaa ggggaactgg
      181 cacggaagct cttcgccatc ggccttttcg acgccgggtt tctcggcgcc ctctgtattt
      241 cccttgccac gtcctggagt ttcggagagg tcttcggatg ggcccactcg ctaaaccaga
      301 gggtcaggga agccccctgg ttttacatcg tgtacctcgg catactggcg acgtcggggg
      361 ccgtggttct cattcccgag gcgccgctcg tgacgattac catgttcgtc caggtcatag
      421 ccgtgacgct tctgcctgcc tctctcgtat tcatgatcat cctgctcaac gacaggtctt
      481 tcatgggaga ccatgtcaac acgggctggc agaacttcgt gaacgggtcg atcatcttgg
      541 tcgtcatcgt catgtcgacg cttatgggat tgaataccct attcccgaat cttttcggct
      601 gatgtcttcg gaggtgtaaa atggctgacc agacgaactc gaaagcaaag gtcgcggata
      661 tcccccgcag catcgggagc taccggttcc tcaatttttc tgagttgata aagaggccgg
      721 tctgtgtagg gaacataaaa aaccgcgtcg gtaggctcac ggacctcgtc ttcgccctgg
      781 cggagcctta ccccgaggtc gtgggtattt acatcgagca cggctggggt aagcccacag
      841 agttcattcc ctggagcaag gtcgtgaaga tcgaagacga cgcgatattc gtgcaccctc
      901 ccgaccaggg cgactcgttc ccgccctttg tggaccaacc cgggtggata ctcctggaca
      961 aacacctcat gggtaggacg atcctggaca tcgacgggcg gagaagcgag gtggtgaacg
     1021 atgtccacct cctcgaggcc aaaggcaggc tccttctcgt ccatgttgac gtatcgatga
     1081 gcggtttctt ccggcgcctc gggctgggaa agctgcgctg ggtgaaggat aacctgattt
     1141 cctggaagta cgtccagccg ctctccgtgg aagacgccat cgcgacagac aaggtgactc
     1201 tctccatcac ccggaagcag ttgctggaac tcccctccga ggacctcgcg gacgttcttg
     1261 aagagctgcc cggcgaggag cagcaggccc tcttttcggc cctcgattcg gaaaaggcgg
     1321 cggagaccct ggtggaagcc gaacctagag cgcagcgccg gatcatcgcc agtctgcgtg
     1381 ccgaccgggc aaggaacata ctttcagaga tgaccgtgcc tcagcttgtc ggcctcttct
     1441 cggttctgcc ccacgacgac gtggagaagc tcatggggct gctcagcaac gaaaaggccg
     1501 agcgcgtcag agatatcctg tccgagaggg aggtccaggc gaaggacatc atgttgccgg
     1561 acttcgttac tgttttcaag acggataagg tcggtgagat tttggagaag atccgccgtt
     1621 cgggccgcga accgaggagc atctcttacg tttacgtggt cgattcggac ggcctgacgc
     1681 ttctcggcgt cgtggacctt cgtgaactcg tccttgccgc ggatcacgtt gaggcagggg
     1741 acatcatggc ctcaccggtt gtcacggcga atgaggacga cctgcaggag gacatcgccg
     1801 ccatgtttgc caagtaccat tacgagatga tccccgtggt ggacgatcag gatcggatca
     1861 tgggcgtcat cctgtatagc gatgtcatca agagcatcga aagccgtgta aaagtataag
     1921 tcagttcata ctatgacggt ttttgttctt aacagcagtc gagggaattg aatgcctcct
     1981 gtcaagatg
//
LOCUS       DAOTMX010000311         1952 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_45467_length_1952_cov_3.902478, whole genome shotgun sequence.
ACCESSION   DAOTMX010000311 DAOTMX010000000
VERSION     DAOTMX010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45467_length_1952_cov_3.902478"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1217
                     /locus_tag="P5573_08130"
     CDS             <1..1217
                     /locus_tag="P5573_08130"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HRT28068.1"
                     /translation="GGNYHPSPGGRGWGRGRETSGGRIWKGRWKMAGIAIDVSPLAPP
                     KTGIGHYVHNLLAHIRDIDRDDEFYLFSRSRFRLEDRIAPAGNTFARFYRIPRYPSPF
                     LWYNLVLPLQVSRHRIDLLYSPTHYTSLVTDVPVVLTVYDLTPLLFPEAHRWDTRLMH
                     KTYFRLSVKKAKVITTISESSKRDIVELLKVPEEKVRIVYPGVERRFAPIPDEDRLRR
                     VAEKYGLPEKFILFVGTLEPRKNLVTLIKAFAGLKKRGIPHRLVVVGKEGWLYAPVFQ
                     VLRDVGLQEEVLLTGYVDEDDLPALYNLACVFVLPSLYEGFGLSLLEAMACGCCVVAS
                     DCSSIPEAVGDAALLFDPHSVQELEGCLLKILEDEGLRKELREKAVERAGRFDWRRSA
                     EEMLGIFGEVAG"
     gene            1219..>1952
                     /locus_tag="P5573_08135"
     CDS             1219..>1952
                     /locus_tag="P5573_08135"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRT28069.1"
                     /translation="MGKRALITGIVGQDGSYLAKLLLEKGYEVWGGDRRSGDGSCRLE
                     DLGIEKDVDVVSMDLLELTNIYKVIEKVEPDEVYNLAAQSFVQASFEMPILTVDVNAL
                     GALRLLEVLRCLRSGAKFYQASTSEMFGNAREVPQTEKTPFYPRSPYGAAKLFAHWIT
                     VNYRESYGMFACSGILFNHESPLRGLEFVTRKITHGLARIKYGLQDRLLLGNLDAERD
                     WGYAPEYVEGMWQMLQHGEPGDYVLAT"
BASE COUNT          432 a          474 c          615 g          431 t
ORIGIN      
        1 gagggggaaa ttatcacccc tcccctggtg ggaggggttg ggggaggggg agagaaacga
       61 gtgggggaag gatctggaaa gggcggtgga agatggcggg gatagcgatc gatgtatctc
      121 ctttggctcc gccgaagacc ggcatagggc actatgtaca caacctcctc gcccacatac
      181 gggacatcga cagggatgat gagttctatc tcttttcgag aagtagattt cggttggagg
      241 accggattgc cccagccggc aacacctttg cgcgtttcta ccgaatcccc cgttatccga
      301 gtccgttcct gtggtacaac ctggtactgc ctcttcaggt atcgaggcat agaatcgacc
      361 tgctatattc tcccacccat tacacttctt tggtgaccga tgtccctgtc gtgttgacgg
      421 tgtacgacct gacgcctctg cttttcccgg aggcccatcg gtgggataca aggttgatgc
      481 acaagaccta cttccggctg tcggtaaaaa aggcgaaggt cataacgacc atatcggagt
      541 catcgaaaag ggacatagtc gaactcctta aggttccgga ggaaaaagtg aggatcgtct
      601 atccgggggt cgagaggagg ttcgcgccga tacccgatga ggaccgtctc cggagagtag
      661 ccgagaagta cggcctcccg gaaaagttca tccttttcgt ggggaccttg gaaccgcgga
      721 aaaacctcgt caccctgata aaggcctttg caggcttgaa aaaaaggggc atcccgcaca
      781 ggttggtcgt cgtcgggaag gagggatggc tctatgcccc tgttttccag gtcttacgtg
      841 acgtaggcct ccaagaagag gtgctgttga ccgggtacgt ggacgaagac gacctgccgg
      901 cgctgtataa tttagcttgt gtttttgtgc taccctcatt atatgaagga tttggtctat
      961 cgttactcga ggcgatggcc tgcggctgtt gcgtggtcgc ttctgattgt tcgtccatcc
     1021 cggaagccgt gggcgacgcc gccctgttgt tcgaccctca cagcgtacag gaattggaag
     1081 ggtgtcttct aaagattctc gaagatgagg gcctgagaaa agagttgagg gaaaaagccg
     1141 tcgagagggc gggacggttc gactggcgca ggagcgctga agagatgctc gggatattcg
     1201 gagaggttgc gggatagcat gggcaagcgg gcgctcatca ccggtatcgt gggtcaggac
     1261 ggctcatacc ttgccaaact cttactggaa aaagggtacg aggtctgggg tggggaccgc
     1321 aggagcggcg atgggagctg ccggctcgag gatctcggca tcgaaaaaga tgtagacgtc
     1381 gtttctatgg atctcctcga attgacgaac atctacaagg tcatcgagaa ggtcgaaccc
     1441 gacgaagttt acaacctggc ggcccagagc tttgtgcagg cgtctttcga gatgccgatc
     1501 ctgaccgtcg atgtcaatgc cctcggcgcc ctgaggctct tggaggtcct gcggtgtctg
     1561 aggtccgggg cgaagttcta ccaggcgtcg acgagcgaga tgttcggtaa tgcacgggaa
     1621 gtgccgcaga ccgaaaaaac gcctttctat cccaggagcc cttacggggc ggctaaactc
     1681 tttgcgcact ggattaccgt gaactacagg gaatcttacg ggatgttcgc ctgttccggc
     1741 atacttttca accacgagtc ccctttgcgg gggctggaat tcgtcaccag gaagattacc
     1801 catgggttgg caaggatcaa atacgggttg caggacagac ttttattggg taacctggac
     1861 gccgagagag actgggggta cgcgccggaa tacgtagaag gcatgtggca gatgttacag
     1921 catggtgagc cgggcgatta tgtccttgcg ac
//
LOCUS       DAOTMX010000312         1946 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_45655_length_1946_cov_3.509783, whole genome shotgun sequence.
ACCESSION   DAOTMX010000312 DAOTMX010000000
VERSION     DAOTMX010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45655_length_1946_cov_3.509783"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="P5573_08140"
     CDS             <1..373
                     /locus_tag="P5573_08140"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRT28070.1"
                     /translation="ALDESIRYSKERVQFGNPISRQQAVAWMIADMATDLTAARFLTY
                     HAAWLKDQKRPFTTESAIAKLFGSEKAMYHTTRAVQIHGGYGYIKGAKVERLMRDAKV
                     TEIFDGTSEAQRMVISFNTLR"
     gene            complement(395..637)
                     /locus_tag="P5573_08145"
     CDS             complement(395..637)
                     /locus_tag="P5573_08145"
                     /inference="COORDINATES: protein motif:HMM:NF021561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2284 domain-containing protein"
                     /protein_id="HRT28071.1"
                     /translation="MRSSRTGITKAFVYLAGPCPLCKECAIIKNEPCVLPGKARPSME
                     SCGIDVFQTARNNGFFIRALREKTETQNFYCLMLVD"
     gene            complement(570..935)
                     /locus_tag="P5573_08150"
     CDS             complement(570..935)
                     /locus_tag="P5573_08150"
                     /inference="COORDINATES: protein motif:HMM:NF021561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2284 domain-containing protein"
                     /protein_id="HRT28072.1"
                     /translation="MQTADLEKYCELAVKAGVTNAKVIHPGTVVTAPWVRMKCLFGCP
                     YRMNHCCPPTTPTPQETRDVLDSYSRAILFHIEASRTPERGKRNLALFDMLVNLEGEI
                     FKDGYYKGLRLSGGPLPSL"
     gene            1134..1760
                     /locus_tag="P5573_08155"
     CDS             1134..1760
                     /locus_tag="P5573_08155"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HRT28073.1"
                     /translation="MRAGVGVPAVSLTGQPGEKANPYGGISRSEFPAKMGGGGIGLAG
                     FAVAATPVAGQVITTPSTGITVADGEVPAGDFQVPVYEGRPAAAGRYPVVLVIPEVFG
                     MHEHIKDVTCRFTMAGFLAITYEPYAREGGVINRPDIDAVRKVVDPIPDGHGLRREEP
                     GCAGRSNRRHGVLPRRVEHASFRRPQSGGEGRRCVVRPDSTRTDARRM"
BASE COUNT          372 a          584 c          594 g          396 t
ORIGIN      
        1 ggccctcgat gaatccatcc ggtattcgaa ggagagggtc cagttcggga accccatctc
       61 ccgccagcag gccgtcgcct ggatgatcgc cgacatggcg acggacctga ccgccgcgag
      121 gtttctcacc taccacgccg cctggctgaa ggatcagaag aggcccttta ccaccgagtc
      181 cgccatagcc aagcttttcg gctcggaaaa ggccatgtat cacaccacca gagcggtcca
      241 gatccacggg gggtatggtt acatcaaggg ggccaaggtg gagcggttga tgcgagacgc
      301 caaggtgacc gaaattttcg acggtacgtc ggaagcccag cggatggtga tctctttcaa
      361 tactctgcgc tgatcagcca gccggcgatc cggttcaatc gaccagcatg agacaataga
      421 agttctgcgt ttctgtcttt tctctcagcg cccggatgaa aaacccgttg ttccttgccg
      481 tctgaaagac atcgatcccg caggactcca tggagggtct tgccttgccg ggaaggacgc
      541 agggttcgtt tttgattatg gcgcattcct tacagagagg gcaggggccc gccagataaa
      601 cgaaggcctt tgtaataccc gtccttgaag atctcaccct ccagattgac gagcatgtcg
      661 aacagcgcaa ggtttcgctt tcccctctcc ggtgttctgg atgcctcgat gtggaacagg
      721 atggcacgcg agtagctgtc gagaacatcc cttgtctcct gcggcgtggg cgtcgtcggc
      781 gggcaacagt ggttcatccg ataggggcat ccgaagaggc acttcatcct gacccagggt
      841 gcggtgacga ccgtgcccgg gtggatgact ttcgcattcg taacacctgc cttgacagcg
      901 agttcgcagt acttttccag atccgccgtc tgcataggac cctcccttct tcgtttattc
      961 gggattcccc ggtatcgccg aaattcaccg ccgcattgcc gtcacgattg cgactgcata
     1021 caggataaga cgatctttgt caaaacgatc tgttccggca ccgcggtccg tctcattctg
     1081 aaaggcgcat ttccacttga cctccggagt cacttccctc atatttctca aaaatgcgcg
     1141 caggtgtcgg ggtcccggcc gtttctttga cagggcaacc cggggagaaa gcgaacccct
     1201 atggaggcat ttcccgcagt gagtttccgg cgaaaatggg aggagggggt atcggtctcg
     1261 cgggttttgc cgttgccgcg acaccggtgg cgggccaggt catcacaaca ccctcaacgg
     1321 gcatcaccgt cgccgacggc gaggtgcccg cgggggattt ccaggtgccc gtctacgagg
     1381 ggcgcccagc cgctgccggc aggtaccctg tcgtcctggt catcccggaa gtcttcggga
     1441 tgcacgagca catcaaggat gtcacctgcc gtttcaccat ggcaggattt ttggctataa
     1501 cgtatgaacc ctacgcgcgg gagggaggcg tcatcaatcg gcccgacatc gacgccgtac
     1561 ggaaggtggt cgacccgatc cccgacggtc acggcctgcg ccgggaggaa cccggctgcg
     1621 cgggtcgatc gaatcggcgt cacggggttc tgccgcggcg ggttgaacac gcttctttcc
     1681 gccgcccaca atcgggaggt gaaggccgcc gttgcgtggt acggccggat tcgacccgca
     1741 cggacgcccg gcgtatgtag cgtcggaccg ctcgatgtag cggcgaagat cgatgcccct
     1801 gtcccgggcc tttacggcgg ggagtgacct gggaatcccc gtggcggatg cgaaggcgat
     1861 agaggcgacc ctcaaggcag ccggcaggac ggccgagttt gtgatctacc ccggggcacc
     1921 gcatgctttc ttcgcggact accggt
//
LOCUS       DAOTMX010000313         1941 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_45812_length_1941_cov_3.915164, whole genome shotgun sequence.
ACCESSION   DAOTMX010000313 DAOTMX010000000
VERSION     DAOTMX010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45812_length_1941_cov_3.915164"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1358)
                     /locus_tag="P5573_08160"
     CDS             complement(<1..1358)
                     /locus_tag="P5573_08160"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRT28074.1"
                     /translation="MENRPKKRRWRPTIGIRTKVLIAFIVLPLVSIAAFGYFALSDIR
                     KIGEYALLSSTVLGESAATDSSRALEQLGETIIAQKAADVALQCKIFIDAHPGMTIED
                     LQHNREFRKIAVQPVGKTGYTILYEKSGIMRFHLNPELINFDMHNWRDKLPDFWHIFE
                     KTFDGRPNSGYYDWQDVDGSIRRKFMAIVPVSGTPYMVAATTYIHEFSIPMEETKKKI
                     SEATRSINAHVNQMLDGIYVTFIHFIIIMVIAILVISFWLSRTITRPIVALTEGAKAI
                     GRGELNYRVEVTTDDEFGTLARAFNRMASDLKEHMAELARTTADKESFRKELEIARRL
                     QRRLLPDRPPSIPGYDIAADNLPAREVGGDFFDFIPMTEDRWGFVIADVSGKGITAAI
                     FMGLSRTIVRATMTRTLDISSSLQQANDLICRDSTSGMFVTLFYAVPIPEERKLRYVN
                     AG"
     gene            complement(1368..>1941)
                     /locus_tag="P5573_08165"
     CDS             complement(1368..>1941)
                     /locus_tag="P5573_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT28075.1"
                     /translation="CALERDIGRIRWTYDPLVSRNGYIYIHKMGATVSEIGFDYYSAV
                     FTSDEFNRGISTDRLIVEWQVDSARVKERFEKGILPPLHDTSEMTEENTVNEIEIDEQ
                     GLEKPGDKMLFNLKTSPLFIEIPFNQDKLLKADRAKAQALRDRIRGLFMHYLARGYVV
                     RGFVVKKEPDGRRRPFYRLERETKFQSLDL"
BASE COUNT          366 a          527 c          553 g          495 t
ORIGIN      
        1 tgcccggcgt tgacgtaccg tagttttctc tcctccggaa tcgggactgc atagaagagc
       61 gttacgaaca tgcccgaggt ggaatcccgg cagatgaggt cgtttgcctg ttgcagggag
      121 gatgatatgt caagggttct cgtcatggtg gcgcggacga tcgttcttga gagacccatg
      181 aagatggccg ccgttatgcc tttccctgag acgtcggcga tcacgaaccc ccaccggtcc
      241 tccgtcatgg ggatgaagtc gaagaaatcc ccgcccactt cacgggcggg caggttgtcg
      301 gcggcgatat cgtatccggg tatactgggc ggccgatcgg gtagcaacct tctctggagg
      361 cggcgggcga tctccaactc tttgcggaaa ctttccttat cggctgtcgt gcgcgccagt
      421 tccgccatgt gttccttgag gtcagaggcc atcctgttga aggcccgggc cagggttccg
      481 aattcgtcgt cggtggtcac ctcgacgcgg tagttcagct cgccgcggcc tatggccttc
      541 gccccctccg tcagggctac gataggtctt gtgatcgtcc gggaaagcca gaaagagatg
      601 acaaggatag cgatgaccat gatgatgatg aagtggatga aggtgacgta gataccgtcc
      661 agcatctggt tcacgtgggc gtttatgctg cgggtagcct cagaaatctt cttttttgtt
      721 tcctccatag ggatggagaa ttcatgtatg taagtcgtcg cagccaccat gtaaggcgtc
      781 ccgctcacgg gcacgattgc catgaattta cgtcggatac tgccgtcgac atcctgccag
      841 tcgtaatatc cactgttggg ccttccgtcg aaggtttttt cgaagatgtg ccagaagtcg
      901 ggcagtttat ccctccagtt gtgcatgtcg aagtttatga gctccgggtt gaggtgaaac
      961 cgcattattc ccgatttctc atagaggatt gtgtagcccg tcttcccgac gggttgcacg
     1021 gctatcttcc ggaattcacg gttatgctgg aggtcctcga tggtcatccc ggggtgggcg
     1081 tctatgaaga tcttacactg aagcgctaca tcggccgctt tctgcgcgat gatcgtttca
     1141 ccgagctgct ccaaggccct tgaactgtcg gttgccgcgc tctctccgag gacggtgctg
     1201 ctcaggaggg cgtattcacc gattttcctg atgtcactga gcgcgaaata gccgaaggcg
     1261 gcgatggaga cgaggggcag cacgatgaag gcgatcagta cttttgtcct gatcccgatt
     1321 gtcggcctcc acctccgctt tttgggtcgg ttttccattg aaaaccatca aaggtccagg
     1381 gactgaaatt tggtctcccg ctccagtcta tagaacggcc ttcttcgccc gtcgggttct
     1441 tttttcacga cgaaacccct gaccacgtaa cccctcgcca gataatgcat aaagaggccg
     1501 cggatcctgt ccctaagggc ttgcgccttc gcacggtcgg ccttcaacag tttgtcttgg
     1561 ttgaagggaa tctcgatgaa gagcggcgac gtcttcaggt tgaagagcat tttgtcgcct
     1621 ggtttttcca ggccttgctc gtcgatctcg atttcgttga cggtgttttc ctctgtcatc
     1681 tcggatgtgt cgtgaagggg gggcaatatc cccttttcga acctttcttt gacccgtgcc
     1741 gaatctacct gccattctac gatcagccgg tccgtcgaga tgccgcggtt gaactcatcg
     1801 ctcgtgaaaa ccgcgctgta ataatcgaaa ccgatctcgc tcaccgtcgc ccccatcttg
     1861 tggatgtaaa tgtagccgtt tctggatacc agcgggtcgt aagtccagcg gatacggccg
     1921 atgtctctct ccagggcgca t
//
LOCUS       DAOTMX010000314         1940 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_45850_length_1940_cov_4.096021, whole genome shotgun sequence.
ACCESSION   DAOTMX010000314 DAOTMX010000000
VERSION     DAOTMX010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45850_length_1940_cov_4.096021"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..162)
                     /locus_tag="P5573_08170"
     rRNA            complement(<1..162)
                     /locus_tag="P5573_08170"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(468..1766)
                     /gene="tyrS"
                     /locus_tag="P5573_08175"
     CDS             complement(468..1766)
                     /gene="tyrS"
                     /locus_tag="P5573_08175"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HRT28076.1"
                     /translation="MEGLLNVYDVFVERGFVSAVTDEKGVRDLLGRERVTCYIGFDPT
                     APSLHVGSLVPIMALVHMQRAGHRPIALVGGGTALIGDPSGKSEMRQVLTHEQVNLNA
                     AALKGQLSRFIHSGTSDGALLLNNADWLTQLNYIEFLRDIGRHFSVNRMLAAESYRMR
                     LETGLNFIEFNYMLLQSYDFYHLFNNYECAVQMGGNDQWGNIVAGIDLIRRLTGKEAF
                     GITFPLITTSLGQKMGKTEKGTIWLDPNLTTPYDYYQYWVNCDDRDVERFLSLFTFLP
                     MDEIREAGRLRDAELNMAKAVLAYEATKIAHGEEAARSARQAAVSVFKAKPVSPGILP
                     SSTIPRGESTTDDSAISRVVKSRAELEKGIPAYKLFTEANLCASQGESRRLISQGGGY
                     INDRKITSFDELIGLKDFSGQPKIYLRKGKKKYSVVEIEG"
BASE COUNT          470 a          562 c          436 g          472 t
ORIGIN      
        1 cgcctttcgg cgagttattc cccactgaag ggcagattac ccacgtgtta cgcacccgtg
       61 cgccacttta ctcatccgcc gaagcggact ttctcgtacg acttgcatgt gttaagcacg
      121 ccgccagcgt ttgttctgag ccaggatcaa actctccagt ttaatggaag tttgtcacta
      181 actttttaat tacaaaggaa aaaggaccca caaatctatt ttcccgctat tcaattttta
      241 aagagctctt cttttcggaa cgagttttgt tttttacagg aacgctaaaa aattgtcaag
      301 cacttttttc actaaaaatt ataccgaaaa ccatcaaaga ccagtaccga aaagggtagt
      361 tttattatac cagattatcc tgggtgtcaa gacaaaaaaa tgaaaaccca aaaaatcagc
      421 aaccgacttc atcgaccggt cgcctgacga agtcaggctt tcctgtatca accttctatc
      481 tcaacaacag aatacttctt cttccccttt cttaagtata tcttcggctg tcccgaaaaa
      541 tcctttagtc ctatgagttc gtcgaaggat gttatctttc tgtcgttgat ataccctcct
      601 ccctgggaaa taagccttct cgactcccct tgagaggcac agaggttggc ctcagtaaag
      661 agtttgtaag caggtatacc tttttccagt tccgccctgg acttcacgac cctggatatg
      721 gccgagtcgt cggtcgtcga ctccccgcga ggtatcgtac tgctgggcaa gatccccgga
      781 gaaaccggct tcgctttaaa gacggataca gccgcctggc gcgccgagcg ggcagcctcc
      841 tcaccatggg ctatcttcgt ggcctcataa gccaggaccg ccttcgccat attgagctcg
      901 gcgtcccgca atcttcccgc ctcccttatt tcatccatcg ggagaaacgt aaacagcgac
      961 aggaaccttt ccacgtccct gtcgtcgcag ttcacccagt actggtaata atcataagga
     1021 gtagtgaggt tcgggtcaag ccagatagtc cccttttctg ttttacccat tttctgcccg
     1081 agagaagttg tgatcaaggg gaaagtgatc ccgaaggcct ccttgccggt caggcgcctg
     1141 atcaggtcga tcccggccac aatgttcccc cattggtcgt ttccgcccat ctggacggca
     1201 cactcatagt tattgaaaag atgataaaaa tcgtatgatt gcaagagcat gtagttgaat
     1261 tcgatgaagt tgaggcccgt ttccaggcgc atacggtaac tctccgccgc aagcatgcgg
     1321 ttcacgctga agtgacggcc gatatcccgg agaaactcga tgtaattgag ctgcgtgagc
     1381 cagtcggcgt tattcaacag cagggctccg tctgaggtcc cagaatggat aaacctcgac
     1441 agctgaccct tcaaggcggc ggcattcaga tttacctgct cgtgcgtcag gacctgtctc
     1501 atttccgact taccgctggg gtcgccgatg agggccgtac ctccaccgac taaggcgatg
     1561 ggccgatgac cggcccgttg catatggacg agggccatga tcggcacaag gctccccaca
     1621 tgcagactag gcgctgtcgg gtcgaaaccg atgtaacaag tcacccgttc cctgcccaga
     1681 agatcccgga cgcctttttc gtccgtcacc gccgaaacga accctctttc aacaaaaaca
     1741 tcgtaaacgt tcaacagccc ctccatacaa ctaaaaatcg ccactgcgcg agatcatcag
     1801 acgtccgccc tcagtctccg agcttcacat cgacacgctc gatggttcgg ctcctcccgg
     1861 tctcttcatc tacatccaca atgacacctt gaaggtgaac atcccgcttg gccacatcaa
     1921 agcgggccgc catacccgtg
//
LOCUS       DAOTMX010000315         1940 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_45853_length_1940_cov_3.802653, whole genome shotgun sequence.
ACCESSION   DAOTMX010000315 DAOTMX010000000
VERSION     DAOTMX010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45853_length_1940_cov_3.802653"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..1839)
                     /gene="ispH"
                     /locus_tag="P5573_08180"
     CDS             complement(106..1839)
                     /gene="ispH"
                     /locus_tag="P5573_08180"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HRT28077.1"
                     /translation="MSVTLAKNAGFCSGVKRAMDIVLDIARHRENEQIYTYGPLIHNP
                     PTIELLKKRGIRPLEDIRDIKGGTVIIRAHGISPLERKMLKGRGVEIIDATCPKVANV
                     QATIKKHANLGYSILIVGDREHPEVEGLLGYAQGRGRVIGTEEDIPALPDTGKVCVVA
                     QTTQSREEYLKIAEKISARYPGALVFDTICDSTELRQTEVKEMAAVMDAVVIVGGKNS
                     ANTRRLVEVVKRRGTAAFHVETAEDLRGIDLSPFSKVGVSAGASTPSWIIEKVVSELN
                     ASRQEKQGGVLHGLLNLWLLAVRSDIYSAVGAGCLLVASSALQGLRVSPVDVAVAALY
                     VLSMHRLNRIMDRKIRGMEDTFSGEIYRRHRKAYVAVTVLSMVAALIIAIFQGIVPFL
                     VLLLITVGGMLYAVRIFPHSWRLERMKDLPGSKNVLTALAWAAVTALIPWVEQSGTPT
                     VDAALAFSFNFALVFVKSVMSDMVNIQGDRLIGRETIPVLVGEKRSRRLVAGISVFLL
                     VLVVSANPLGWTSSVSFALLTCVFYVWICLYLYDRRAKFSGAVLEGLLQTNFIIAGAS
                     TLFWISIVSRT"
BASE COUNT          427 a          606 c          501 g          406 t
ORIGIN      
        1 cgtaacggag gtttgccccc ccgcaacccg ccagcagcaa ggtcatagcg acggcaatca
       61 gcgcccggaa caacctgtct gtcatgatca cgaccccctt ttgtatcatg ttcgcgagac
      121 gatcgaaatc cagaataacg tactcgctcc cgcgattatg aagttggtct gtagtaatcc
      181 ttccaggacc gcccctgaaa atttcgccct tctatcataa agatagagac aaatccacac
      241 ataaaagacg cacgtgagca gtgcaaaact caccgacgac gtccatccca gcggattggc
      301 cgacaccacc agcaccaaca gaaaaacaga tatacccgca acgagacgcc gggaccgttt
      361 ctccccaacc agaacgggga tggtttctct cccgatcagc cggtcaccct ggatattgac
      421 catatcggac ataaccgatt tgacgaaaac gagggcgaaa ttgaaggaaa acgccagagc
      481 tgcgtcgacc gtcggggtcc cgctctgctc cacccagggg atcagggcag ttacggctgc
      541 ccacgccaga gcggtcaaca cattcttgga accgggcagg tctttcatcc gctccagtcg
      601 ccagctatgg gggaaaatcc tcacggcgta aagcatcccc ccgaccgtga tcaatagcag
      661 cacgaggaag ggaactattc cctgaaaaat ggctatgata agggctgcaa ccatcgacag
      721 cacggttacg gcgacatagg ccttccggtg cctccggtag atttctccac tgaaggtatc
      781 ctccatccca cgaattttcc tgtccatgat ccggttcaag cgatgcatcg ataaaacata
      841 tagggcagcg acggcgacgt cgaccggact caccctgaga ccctgaaggg ccgagctggc
      901 aacgaggaga caaccggcgc ccactgcgga atagatgtcg cttcttaccg cgaggagcca
      961 aagattcaac agaccatgga gcaccccccc ctgcttttct tgccgggatg cgttgagttc
     1021 actgacgact ttctcgatta tccagctggg cgttgaggcc ccggcggaga caccaacctt
     1081 gctgaaagga ctgaggtcga taccccttag atcttccgct gtttcgacat gaaaagccgc
     1141 cgtgccccga cgtttcacga cctctacaag acgcctggtg ttggcgctgt tcttcccgcc
     1201 cacgatcacc acggcatcca tgacggcggc catctccttc acctcggtct gtctcaactc
     1261 ggtggaatcg cagatggtgt cgaagaccag ggcgcccgga taccgggcgg aaattttttc
     1321 cgcgatcttg aggtactcct ccctgctctg cgtggtctgg gcgacaacac agaccttgcc
     1381 ggtatccggc agggccggga tgtcttcctc cgtcccgatc acccttcccc ttccctgcgc
     1441 gtacccgaga agcccctcga cctccgggtg ttccctgtct cccacgatca agatgctgta
     1501 acccaagtta gcgtgcttct tgatagtcgc ctggacattc gccactttgg ggcaggtggc
     1561 atcgatgatc tcgacccctc ttcccttcaa catcttacgt tccaggggag agatgccgtg
     1621 ggcccggatg atgaccgtac ccccctttat gtcacggatg tcctccagag ggcggatacc
     1681 ccttttcttc agcagctcga tggtcggcgg gttgtggatg agcgggccgt aggtgtagat
     1741 ctgctcgttc tccctgtgac gggcgatgtc gagcacgatg tccatggccc ttttgacgcc
     1801 cgaacagaag cccgcatttt ttgcaagcgt aacgctcata gcttaagttt cagccggctc
     1861 catcgccgcc gaagacctga agttctcttc cttgacggcg tggacgaaga attctttgtt
     1921 gcctgccggt ccgagaagcg
//
LOCUS       DAOTMX010000316         1913 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_46765_length_1913_cov_3.577503, whole genome shotgun sequence.
ACCESSION   DAOTMX010000316 DAOTMX010000000
VERSION     DAOTMX010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46765_length_1913_cov_3.577503"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="P5573_08185"
     CDS             complement(<1..821)
                     /locus_tag="P5573_08185"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HRT28078.1"
                     /translation="MRRNWRNLIKPKRIEVDEKTHTPFYGEFTCQPLEKGFGITLGNA
                     LRRILLSSIYGSAIVSVKFDGVLHEFSTIPGVKEDVSDIILNLKGVRFKLHGDGARTV
                     HLDVSREGAVTAGDIKTDGTVEILNPDHHIATLAPGATLKAEMTVKTGKGYVPAKMER
                     EPDQPEGTINIDAIFSPIKKVAYNVTYARVGQITDYDRLVMEIWTDGSILPEDAVSYA
                     ARILKDQLDVFTNFEEAEEDERQDRVDEKEKINENLMRSVDELELSVRSANCLKNA"
     gene            complement(855..1481)
                     /gene="rpsD"
                     /locus_tag="P5573_08190"
     CDS             complement(855..1481)
                     /gene="rpsD"
                     /locus_tag="P5573_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HRT28079.1"
                     /translation="MARYRGSLCRLCRSEGTKLFFKGDRCYTDKCGFERRGYAPGEHG
                     QSRRKQSDYGVQLREKQKLKRMYGLQERQFRNYFEKAEQQKGVTGTNLLIFLERRLDN
                     MVYRLGFANSRTEARLLVAQNHFMVNGEKVNIPSYLVGAGDVISVREKSRKVARILES
                     METVARRGVPHWLELDKENFRGTVKMLPAREDLTMPMQEQLVVELYSK"
     gene            complement(1718..>1913)
                     /gene="rpsK"
                     /locus_tag="P5573_08195"
     CDS             complement(1718..>1913)
                     /gene="rpsK"
                     /locus_tag="P5573_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HRT28080.1"
                     /translation="AAEGAVKKAVEHGVRTVQVYVKGPGSGRESALRALQAAGLNVSL
                     IRDVTPVPHNGCRPPKRRRV"
BASE COUNT          333 a          600 c          494 g          486 t
ORIGIN      
        1 gcgttcttga ggcagttggc cgagcggacg gagagctcca gctcgtctac actccgcatg
       61 aggttttcgt tgattttttc cttttcgtcg accctgtcct gtctctcatc ctcttccgct
      121 tcttcgaaat tggtgaagac gtcgagctga tccttcagga tcctggccgc gtaagagacg
      181 gcgtcttcgg gaaggatgct gccgtcggtc catatctcca tcaccagccg gtcgtagtcc
      241 gttatctggc cgaccctggc gtaggtgacg ttgtaggcga ccttcttgat gggagagaag
      301 atggcgtcga tgttgatcgt cccttccggt tggtcgggct ctctttccat cttggccggg
      361 acgtagccct tccccgtctt cactgtcatc tcggccttca acgtcgcccc gggggcgagc
      421 gtggcgatgt ggtggtcggg gttgagtatc tccacagtgc cgtccgtctt gatgtcgcct
      481 gccgtgaccg cgccctcccg ggagacgtcg aggtggacgg tacgggcccc gtcaccgtgc
      541 agcttgaacc gcacaccctt caggttcagg atgatgtcgc ttacgtcttc cttcaccccc
      601 ggtatggtcg agaactcgtg cagaactccg tcgaacttga cagagacgat cgccgacccg
      661 tagatagacg aaaggaggat ccttctgagc gcgttgccga gggttatccc aaatcccttc
      721 tccaggggct gacaggtgaa ttcaccgtaa aagggcgtgt gggtcttctc gtcaacctcg
      781 atcctcttcg gctttatcag attgcgccaa tttcttcgca ttgcttcgct tcctcgtgtc
      841 atacggctat cccatcactt tgagtaaagc tcgaccacca gttgctcctg catcggcatg
      901 gtcaaatctt cccgtgccgg cagcatcttc acggtacccc tgaagttttc cttgtccagc
      961 tccagccagt gaggtacgcc ccgtctggcg acggtctcca tcgattcgag tattcgcgca
     1021 accttcctgc tcttttcccg taccgagatg acatcccccg ctcctaccag gtaggagggg
     1081 atgtttactt tttccccgtt caccataaag tggttctgcg cgacaagcag tcttgcctcc
     1141 gtccgggagt tcgcaaaccc gaggcggtag accatgttgt cgagcctcct ctcgaggaag
     1201 atcaggaggt tcgttcccgt cacgcccttc tgttgttccg ccttctcgaa atagttcctg
     1261 aactgcctct cctgcaggcc gtacatcctc ttgagtttct gcttctcccg gagctgtacg
     1321 ccgtaatccg actgcttccg gcgggactga ccgtgttcgc ccggggcgta accgcgtctc
     1381 tcaaagccgc acttatccgt gtagcaccgg tcacctttga aaaaaagctt ggtgccttcg
     1441 ctccggcaca acctgcataa cgaacctctg tatcttgcca acttatcctc ccgttaagtc
     1501 aaatcccaat cgtaaatctc tcgtaaagaa tcagagaaga catctttttg ccgttccggc
     1561 gcttcacccc gattaaaatc ggggcggtat ccagggctta ccccgtcata ccggcgtaag
     1621 ccggtatcca gtatattcaa tttgtcctgg atgccggatc gagtccggca tgacctcttc
     1681 ggttttttgt atgagtacac aaagttgcag cattcagtta cacccgccgc cgtttcggag
     1741 gcctacagcc gttgtgagga acaggcgtga cgtccctgat cagactgacg ttcaggcccg
     1801 ccgcctgaag ggcccgcagt gccgattccc tccccgaacc gggccccttc acgtagacct
     1861 gtacggtcct gacaccgtgc tcgacggcct tctttaccgc cccttcggcg gcc
//
LOCUS       DAOTMX010000317         1901 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_47152_length_1901_cov_2.639220, whole genome shotgun sequence.
ACCESSION   DAOTMX010000317 DAOTMX010000000
VERSION     DAOTMX010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47152_length_1901_cov_2.639220"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="P5573_08200"
     CDS             complement(<1..256)
                     /locus_tag="P5573_08200"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRT28081.1"
                     /translation="MIYLDNAATTWPKPDTVWEAVVAGGKQKCGNPGRSGHRLAVEAA
                     REVFAAREAVAELFNTGDSSRIVFTWNATGALNTALQGFLR"
     gene            complement(253..852)
                     /gene="yedF"
                     /locus_tag="P5573_08205"
     CDS             complement(253..852)
                     /gene="yedF"
                     /locus_tag="P5573_08205"
                     /inference="COORDINATES: protein motif:HMM:TIGR03527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HRT28082.1"
                     /translation="MSKKIIDCRGLSCPRPVLETKKVLDEYSGEDVDVIVDDETACQN
                     VSLYAAGRNWAVCHIGREGTIIRLSLKRKTAEACETMASAQAKQEMLLVYLSSETIGR
                     GDDELGKLLMRSFIQALFETPPLPKRMVFLNSGVKLATEGSLVLDALRAFEDKGVEIV
                     SCGTCLDYFRLKDKLRVGKVTNMFEIISSLQSFEKVVQP"
     gene            complement(871..>1901)
                     /locus_tag="P5573_08210"
     CDS             complement(871..>1901)
                     /locus_tag="P5573_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HRT28083.1"
                     /translation="MEVIRAAGAVPAILCAFANKTIAAAEAVLPANLCPLIKSSYGFI
                     ITDTCPFFALSDAIIAETTCDGKKKMFELIADYKPMHVMDLPQLPDEEEARKNWTAMI
                     RKVATFLEKTFNCSISEDGIEEEIRATNRKNRLMQRIYDFSAEKPPVLSWGENYELGL
                     LSQVASTVDVTPVLEKAIESLEKRKAAGIHYGMKSAPRVLVTGCPLGGDSMKVYRIIE
                     EVGGVVVAMDACTGMKPFMSDIEEGTGDPIASVARRYLKLPCSCMTPNLRRLRELDGL
                     VERFKPDVVVDVVLHACHSYNVESYKIMNHVKDRYGLPFLKIETDYSDGDVEQIRARV
                     EALFDSLN"
BASE COUNT          379 a          568 c          521 g          433 t
ORIGIN      
        1 tcctcaggaa cccctgcagg gccgtattca gggcccccgt ggcattccac gtaaatacga
       61 tccgcgagga atcgccggtg ttgaacagct ctgcaacggc ttcccgggcg gcaaaaacct
      121 cgcgggccgc ttccaccgcc aggcgatgac cgcttcgccc cggattgccg cacttttgtt
      181 ttccgccggc gacgacagcc tcccataccg tgtccggttt cggccaggtc gttgccgcat
      241 tgtcgagata tatcaaggct gcaccacttt ttcgaaactc tgcagggaag aaatgatttc
      301 gaacatgttg gtcactttcc cgaccctgag cttgtccttg aggcggaaat aatcaagaca
      361 cgttccgcag gacacgatct cgacaccttt gtcctcgaag gccctcaatg catccagcac
      421 cagggacccc tccgttgcga gcttcacgcc ggagttgagg aagaccatgc gcttcggcaa
      481 gggcggcgtt tcaaaaagcg cctggataaa gctcctcatc agtaattttc cgagttcatc
      541 gtcaccgcgt ccaatcgtct ccgatgacag gtaaacgagg agcatctcct gttttgcttg
      601 agcagatgcc atggtctcgc aggcttcagc ggtcttgcgc ttcaaggaaa ggcgtatgat
      661 tgtcccctct ctgccgatat ggcacaccgc ccaattgcgc ccggcagcat agagcgatac
      721 attctggcat gcggtttcgt cgtctacaat gacgtcgaca tcctctcccg aatactcgtc
      781 caggactttc ttcgtctcca acacgggccg tgggcaggac agcccccggc aatcgatgat
      841 tttcttgctc atgggcaact ctcctccctt tcagtttagg ctgtcgaaaa gagcctcgac
      901 ccgcgcccgg atctgctcga catccccgtc ggaatagtcc gtctcgatct ttaggaaggg
      961 aagcccgtac cggtccttca catggttcat gatcttgtag gactccacgt tgtaggaatg
     1021 acaggcgtga aggacgacat cgacgacgac atccggctta aaccgttcaa ccaggccgtc
     1081 gagttcgcga agtcttctca gattgggcgt catgcatgag caggggagct tgaggtaacg
     1141 tctcgcgacc gaggcgatgg ggtcccccgt gccctcctca atgtcagaca tgaatggttt
     1201 catccccgtg caggcatcca tcgccacaac cacgccgccc acttcttcga tgatcctgta
     1261 gaccttcatc gaatccccgc caagagggca gcctgtgacc agcacccggg gcgctgactt
     1321 catgccgtaa tgaatgcccg cggccttcct tttctccagg ctctcgatcg ccttttccaa
     1381 aacgggggtc acgtcgaccg tggaggccac ctgggaaagg aggcccaatt cgtagttctc
     1441 gccccagctg agaacgggtg gcttttcggc ggaaaagtcg taaatccgtt gcatcagccg
     1501 gttcttgcgg ttcgtcgccc tgatctcttc ttcgatgccg tcttcagata tgctgcaatt
     1561 gaaggtcttc tcgagaaagg ttgccacttt tcggatcatg gcggtccagt tcttccgggc
     1621 ttcctcctcg tcgggaagct ggggtagatc catgacgtgc atgggtttgt aatcggcgat
     1681 cagttcgaac atcttctttt tcccgtcgca ggtcgtctcc gcaatgatgg catcggagag
     1741 cgcaaaaaac ggacaggtgt cggtgatgat gaaaccgtag ctggatttga tgagagggca
     1801 gaggttggcc ggcaggacgg cttccgcagc ggcgatcgtc ttgttggcaa aggcgcacag
     1861 gatggcaggc acggcgccgg cggcccggat gacttccatg g
//
LOCUS       DAOTMX010000318         1892 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_47444_length_1892_cov_4.132281, whole genome shotgun sequence.
ACCESSION   DAOTMX010000318 DAOTMX010000000
VERSION     DAOTMX010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47444_length_1892_cov_4.132281"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="P5573_08215"
     CDS             complement(<1..209)
                     /locus_tag="P5573_08215"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRT28084.1"
                     /translation="MNINVLIADDHAIVRDGLRYLLEAQGDIKVVGEAATGREALKKV
                     NELKPDIVIMDIVMAELNGIEATEQ"
     gene            complement(206..1753)
                     /locus_tag="P5573_08220"
     CDS             complement(206..1753)
                     /locus_tag="P5573_08220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRT28085.1"
                     /translation="MPTKNKAKHELIEEVSSLRQKIAELERANIGLLKEAEELRKSEG
                     KYRNLVESTLDLIFMVDEKGRYTYVNPRFEKVTGYSIDDLIGLPFIHIAAPEVIRSTI
                     DRFKRGMHGEDTPPYEADLIHKNGDRIPVEFLVTTQYDDRGIPTGRLGIGRDITERRK
                     ADHALRQLAADLAKAQFLAHIGNWRYDINTKLSVWSEEMFRIVGLEPMPNTPLRIDHR
                     RYIHPDDLPKYEEAARSVLAGKQSEVELRVLLPDGSLCYVFVRGEPEYDRNGRIKGLF
                     GTTQDVTGRVMAEQMLKAQSEQLRILSAKLSEAQEAERKRIARELHDQVGQNLTVLGI
                     NLNILRSAVSRDSLTARHNCLLDDSLLLVEQTSEFTRDLMADLRPPDMDDYGLIAAIQ
                     WYSERFSMRTGIEVMMKGDEIHPRPSAYVENNLFRVVQEALTNISKHSRAKKIQIRIS
                     VAEDRLKIKITDDGVGFNPEKSRMSPERRCLGLMSMSERTESMGGRFSVRSRTGKGTE
                     IAVEVPL"
BASE COUNT          333 a          549 c          493 g          517 t
ORIGIN      
        1 atctgctcgg tggcttcgat tccgttcaat tccgccatga cgatatccat gatcactatg
       61 tcgggcttga gttcgttcac tttcttcaac gcttcgcgac cggttgcggc ttcgccgact
      121 actttgatgt ccccctgtgc ttccaaaaga tagcgaagtc cgtcgcgaac gatcgcgtgg
      181 tcgtcggcaa tcaggacgtt gatgttcata gaggcacctc tacagcgatc tccgtaccct
      241 tcccggtcct ggatctgact gagaatcgac cccccatcga ctcggtgcgc tcggacatgc
      301 tcatcagacc caggcaccgg cgttcagggg acatgcggga cttttcaggg ttgaacccga
      361 ccccgtcgtc ggttatcttg atcttcaatc tgtcttcggc gacgcttatt cttatctgta
      421 tcttttttgc tcgggagtgc ttgctgatgt tcgtaagggc ctcctgaacg acccgaaaga
      481 ggttgttttc tacgtaggcg ctcggcctgg ggtgtatttc atcgcctttc atcatcactt
      541 cgatcccggt cctcatcgag aagcgctcgc tgtaccactg aatggcagcg atcaagccgt
      601 aatcgtccat gtcgggcggc ctcaagtcgg ccatcaggtc gcgggtaaat tccgaagtct
      661 gctctacaag cagcagcgag tcgtccaaca gacaattgtg acgcgccgtt aagctgtcgc
      721 ggctgaccgc cgaccgcagg atgttcaggt ttatccccaa gacggtcaga ttctgtccta
      781 cctggtcgtg gagttccctc gctatcctct tcctttccgc ctcctgggcc tcggagagtt
      841 tcgcggaaag gatcctgagt tgttcgcttt gcgctttcag catctgttcg gccatgacgc
      901 ggccggtcac atcctgggtc gttccaaaga gacctttgat ccttccgttc cggtcatact
      961 ccggctcccc ccggacgaag acatagcaca gtgatccgtc ggggagcaaa acccttagtt
     1021 caacttccga ttgctttcct gcgaggacag accgggcggc ctcttcgtat ttgggcaggt
     1081 catcggggtg gatgtatctt ctatggtcga tccgcaacgg cgtattcggc atcggttcga
     1141 gccctacgat gcggaacatc tcttcggacc agacggacag cttggtgttt atgtcgtacc
     1201 gccagttgcc gatgtgggcg agaaactggg ctttcgccag gtcggcggcg agctgcctca
     1261 gggcatggtc ggcctttctg cgttctgtga tgtctcggcc gattcccaat ctgcccgtcg
     1321 gaataccccg gtcgtcgtat tgtgtcgtca caaggaattc taccggtatc ctgtccccgt
     1381 ttttgtggat caggtccgcc tcataggggg gcgtgtcctc gccgtgcatg cctctcttga
     1441 atcggtctat cgtagacctg atgacttccg gggctgctat gtggatgaaa ggcaatccga
     1501 tcaggtcgtc gatggaataa cccgtgactt tttcgaacct cgggttgacg taggtgtacc
     1561 tgcctttctc gtcgaccatg aagatcaggt ccagggtcga ctcgacgagg tttcggtatt
     1621 tcccctcgct ttttctcagt tcctctgcct ccttgaggag gccgatattg gccctctcca
     1681 gctctgcgat tttctgccgc agcgaagaga cctcttctat gagctcatgc ttggccttgt
     1741 tttttgtcgg catcttcgtg ctgcctcaac tattggagtt ctgatatcta taccacaaac
     1801 ccgtcgattt gaaatacctc ttgccataac acgtcaattt ttcaacctcg gcatactcca
     1861 tttgtcatcc ttggcctaca gacagggctt aa
//
LOCUS       DAOTMX010000319         1880 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_47852_length_1880_cov_3.779178, whole genome shotgun sequence.
ACCESSION   DAOTMX010000319 DAOTMX010000000
VERSION     DAOTMX010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47852_length_1880_cov_3.779178"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="P5573_08225"
     CDS             complement(<1..688)
                     /locus_tag="P5573_08225"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HRT28086.1"
                     /translation="MNSRLLLLFSSLLLLIIPSAPAYASTPWAAIAQGVEYAVFNGQI
                     HVVRLDPSRAKLVLLLASEQDKRPRTTGAWCRDFNLIAAINAGMYGTDYSTNVGYLRN
                     GAHVQNGRWSSRYKSALAFDPQKPGLPQAIIVDLDEPGAKDSLKHYRSVVQNLRLMKK
                     GGINVWPPSDKRWSEAAVGMDSQGRILFLFCRTPYPMKEFNELVNSLQLGVTRMMHVE
                     GGSEASLSINT"
     gene            complement(685..1182)
                     /locus_tag="P5573_08230"
     CDS             complement(685..1182)
                     /locus_tag="P5573_08230"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRT28087.1"
                     /translation="MKAKSIQERYPDEFAHCYGCGRLNRKGLRIKSYPEDDECVCRYT
                     PEPYHTGGIPGYAYGGFIASLIDCHSSATASAAKLRLSGSSAGDMAPPRFVAASLKVD
                     FLKPTPVDKVLELRGKVKEMKGRKVIVVVTLSADGKVCARGESVLIEMPPVSGDTARL
                     SGPEI"
     gene            complement(1217..1495)
                     /locus_tag="P5573_08235"
     CDS             complement(1217..1495)
                     /locus_tag="P5573_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRT28088.1"
                     /translation="MSYSLTFKESALKEWGKLDRPIREQFKKKLAKRLDFPRMEADRV
                     GGMDDLYNTKLRAVGLRLVYVVEEETKTLTVYAVGKRDKSCVSLSDDV"
     gene            complement(1485..1733)
                     /locus_tag="P5573_08240"
     CDS             complement(1485..1733)
                     /locus_tag="P5573_08240"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HRT28089.1"
                     /translation="MDYLAAKSSVVSITDLKKNPSAIIKEAEGSPVVILNHNRPFAYI
                     VPADAFEAMLEKLDDLELRKTIRKQQGEKSVPVSLDVL"
BASE COUNT          386 a          542 c          509 g          443 t
ORIGIN      
        1 ccgtattgat ggaaaggctg gcttcgctcc ccccttcgac gtgcatcatc ctggtaaccc
       61 cgagctgcag ggaattgacc aactcgttga attccttcat cgggtacggt gtacggcaga
      121 aaagaaacag aatacgaccc tgactgtcca tgccgacggc cgcctcgctc caccttttat
      181 cggaaggagg ccacacattt atcccccctt ttttcatcag acggagattt tggaccacgg
      241 accggtagtg cttcaaagag tccttcgcgc cgggttcgtc gaggtcgacg atgatcgcct
      301 gggggagccc cggtttttgt gggtcgaagg cgagggccga tttgtacctg ctgctccatc
      361 ttccgttctg tacatgagcg ccgtttctga ggtagcccac gttcgtcgaa tagtccgtcc
      421 cgtacatccc cgcgttgatc gctgcgatga ggttgaaatc cctgcaccac gcaccggtcg
      481 ttcgcggtct tttgtcctgt tccgatgcca agaggagcac gagtttcgcc cgggaagggt
      541 caaggcggac gacatggatt tgtccgttaa aaacggcgta ttcgacaccc tgagcgatgg
      601 cggcccaggg agtggaagcg tatgccgggg cggacggaat gatcaggaga agaagcgacg
      661 aaaacagcaa cagcagacgg gaattcatat ctccggtccg gataaacggg cggtatctcc
      721 cgacactggc ggcatctcga tcagcaccga ctcgcctctg gcgcatacct tgccgtcggc
      781 ggagagcgtc acgaccacga tgaccttgcg gcctttcatt tccttgacct ttcccctgag
      841 ctccaggacc ttgtccaccg gcgtcggctt gagaaagtcg accttcaacg acgccgccac
      901 aaagcgaggg ggcgccatgt cacccgccga cgagcctgac aggcgcaact ttgccgccga
      961 ggcggttgcg gagctgtggc agtcgatcaa cgacgcgata aaaccgccgt aggcatagcc
     1021 ggggatgccc ccggtgtggt aaggctcagg cgtataacga cagacgcatt cgtcgtcttc
     1081 cgggtaactc tttatgcgca gtcccttccg gttgagtcgt ccgcacccgt agcaatgggc
     1141 gaattcgtcg gggtagcgct cctggatgga ttttgctttc atgccggctc tcctctcctg
     1201 aaaaattgcc gtacactcat acgtcatccg acagcgatac gcaactttta tccctttttc
     1261 ccaccgcgta gaccgtcaat gtctttgtct cttcctcaac gacatagacc agccgcaggc
     1321 cgacggcacg gagcttggta ttgtaaagat catccatacc gccgaccctg tccgcttcca
     1381 tgcgcgggaa atccagccgt ttcgccaact ttttcttgaa ctgttcccgg atcggccggt
     1441 ccagtttgcc ccactcctta agggccgatt ctttgaaggt gaggctataa gacatcgaga
     1501 gagaccggaa cggacttttc gccctgttgc ttgcggatgg ttttcctgag ttcgaggtcg
     1561 tccagctttt ccagcatggc ctcgaaggca tcggccggca cgatgtatgc gaaaggtcgg
     1621 ttgtggttga gaatcaccac cggcgacccc tcggcttctt taatgatagc cgaagggttc
     1681 tttttcaggt ccgtgatgct taccaccgac gactttgccg ctaaatagtc catggttctt
     1741 cctccaataa aaccaattta cggcactaaa tatatgtcat atcggcggag agggcaaatg
     1801 atttaacggg ggatttcact actgcccgga ataatctgtg aataagttca actggttagt
     1861 atttttgtcc ccatcgtttc
//
LOCUS       DAOTMX010000320         1875 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_48028_length_1875_cov_3.247253, whole genome shotgun sequence.
ACCESSION   DAOTMX010000320 DAOTMX010000000
VERSION     DAOTMX010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48028_length_1875_cov_3.247253"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /gene="rplF"
                     /locus_tag="P5573_08245"
     CDS             complement(<1..277)
                     /gene="rplF"
                     /locus_tag="P5573_08245"
                     /inference="COORDINATES: protein motif:HMM:NF012567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HRT28090.1"
                     /translation="MSRIGKKPIVVPADVKVSEQGRVIRVEGPLGTLSREIPMGFEVS
                     TEAGVLTLKRLDESRRTRSLHGLMRTLVANMVTGVSTGFQKALEISGI"
     gene            complement(316..717)
                     /gene="rpsH"
                     /locus_tag="P5573_08250"
     CDS             complement(316..717)
                     /gene="rpsH"
                     /locus_tag="P5573_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HRT28091.1"
                     /translation="MGMTDPIADMLTRIRNANGAKLKSVDVLLSQENFNIAKVLKKTG
                     YISGFNVKRDSNQKGVLRIYLRFSDSKKGVITGIERVSRPGKRVYVKSEKIPKIFNGY
                     GVAVLSTSKGIVTDREARDLNVGGELLCKVW"
     gene            complement(744..929)
                     /locus_tag="P5573_08255"
     CDS             complement(744..929)
                     /locus_tag="P5573_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HRT28092.1"
                     /translation="MAKKSLIAKAKREPKFSVRRYNRCPICGRPRAYYRKFDMCRICL
                     RRLALRGEIPGVIKASW"
     gene            complement(958..1497)
                     /gene="rplE"
                     /locus_tag="P5573_08260"
     CDS             complement(958..1497)
                     /gene="rplE"
                     /locus_tag="P5573_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HRT28093.1"
                     /translation="MARLKTYYEKEVVPALVKEFSYRNRVEVPRMEKIVVNMGLGEAI
                     QNIKILDSAVRELAMIVGQRPVVTKAKKSIATFKLRKGMSIGCMVTLRRERMYEFFDR
                     LVNVALPRIRDFRGVSPKSFDGRGNFSLGIKEQFIFPEIDYDKVDKVMGMNITVVTTA
                     KTDDEARRLLKLMGVPFRS"
     gene            complement(1512..1832)
                     /gene="rplX"
                     /locus_tag="P5573_08265"
     CDS             complement(1512..1832)
                     /gene="rplX"
                     /locus_tag="P5573_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HRT28094.1"
                     /translation="MLETRLKKGDMVKVVAGKEKGKSGKILRIIGKKNRVTVEKLNFI
                     KKHQKPDAKGKGGILQKEGPIHISNVMYLCGKCNSAVRVGYRVLEDGRKVRTCKKCNE
                     LLDE"
BASE COUNT          355 a          601 c          419 g          500 t
ORIGIN      
        1 cgatgccgga aatctcgagg gctttctgga agccggtgct cacgcccgta accatattcg
       61 cgacaagcgt cctcatcagg ccgtggagcg acctcgtccg gcggctctca tccagcctct
      121 tcaaggtcaa cacgcccgct tccgtcgaga cctcgaaccc catcggaatc tcccgtgaga
      181 gcgttcccag aggaccctct acccttatga ccctgccctg ctcgctcact ttcacgtcgg
      241 caggaacgac gatcggtttt ttcccaattc ttgacatgtg gcttctctcc tgaacccgga
      301 ccttctaccc gctctctacc aaaccttgca gagaagttct ccgcccacgt tgaggtccct
      361 tgcttccctg tcggtgacaa tgcccttaga agtcgaaagg acggcgaccc cgtaaccgtt
      421 gaaaatctta gggatctttt cactctttac gtaaacccgc ttccccggcc ggctcaccct
      481 ttcgatgccg gtgatgacgc ccttcttcga gtccgagaag cgcagataga ttctcagaac
      541 ccccttctgg ttgctgtcgc gcttcacatt gaaaccgctg atgtatcccg tctttttcag
      601 gaccttggcg atgttgaagt tctcctgaga gagcagtacg tcgacactct tcaatttcgc
      661 accgttggcg tttctgattc ttgtcaacat gtccgcgatc ggatcagtca tccccataaa
      721 ctaaactcct cgattctctt tcttcaccag cttgccttga tcacgccggg tatctcgcct
      781 ctcagggcga gccgcctcaa gcatatccgg cacatatcga attttctgta gtatgcccgt
      841 ggcctgccgc aaatcggaca gcggttgtat ctgcggaccg aaaacttcgg ttctcttttc
      901 gccttcgcaa tcaaggattt cttcgccacc tgtacctccg cttatctcac ttcatgccta
      961 acttctgaaa gggacgccca tcaacttgag taaccgcctc gcctcgtcgt ccgtcttggc
     1021 cgtcgtcacc accgtgatgt tcatccccat gaccttgtcg accttgtcgt aatcgatctc
     1081 cgggaagatg aattgctctt ttatgccgag ggaaaagttt ccccgcccgt cgaacgactt
     1141 cggcgacacg ccccggaagt cccgtatccg gggaagggct acgttgacca gcctgtcgaa
     1201 gaactcgtac atccgttccc tcctgagggt aaccatgcag ccgatggaca tgcccttgcg
     1261 gagcttgaag gtcgcgatag actttttcgc cttcgtgacc accggcctct ggccgacgat
     1321 catggccagt tcccggactg cgctgtcgag gatcttgatg ttctggatgg cttcgccgag
     1381 ccccatgttc accacgatct tctccatccg cgggacttcc accctgtttc tgtagctgaa
     1441 ttccttgacc agggccggaa ccacttcttt ctcgtagtag gttttgagtc ttgccatcag
     1501 attacctcgg cctattcatc caaaagctcg ttgcatttct tgcaggtacg gaccttcctg
     1561 ccgtcttcca ggacacggta tccgaccctg acggcgctgt tgcattttcc gcaaagatac
     1621 atcacgttgg agatatgaat cggaccctcc ttctgcaaga tgccgccttt tcctttcgca
     1681 tcgggcttct gatgcttttt gataaaattc aacttttcca ccgtgacacg gttcttcttg
     1741 ccgatgatcc tgagaatctt accggacttg cccttctctt tgccggcgac caccttgacc
     1801 atgtcaccct ttttcagacg tgtttccagc atttccaaac cctcgactat ttgataacgg
     1861 ctccggcaaa gaccg
//
LOCUS       DAOTMX010000321         1869 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_48258_length_1869_cov_4.156560, whole genome shotgun sequence.
ACCESSION   DAOTMX010000321 DAOTMX010000000
VERSION     DAOTMX010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48258_length_1869_cov_4.156560"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="P5573_08270"
     CDS             <1..322
                     /locus_tag="P5573_08270"
                     /inference="COORDINATES: protein motif:HMM:NF026064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HRT28095.1"
                     /translation="TGQRVMKIFEAIETAYVQYTRRVQTAELNVTVRGIVEKNPPSRH
                     LGRPNSVSYVTQTSVKPPTFVFFVREPKAVLPSYERYLANQLRDALGFQYTPLRLIFR
                     RKRR"
     gene            complement(426..848)
                     /locus_tag="P5573_08275"
     CDS             complement(426..848)
                     /locus_tag="P5573_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRT28096.1"
                     /translation="MMKVMKVRTKGQKGFTLVELMIVVAIIGILAAIAIPQFAAYRER
                     GYIASMQADLNSVRLAEEAYYAQNNTYVEGTATDQAAIDALKDFGGVKASAGNTVTVT
                     PYTEDGKSYFKVEVTSTKAGSKVTYDSKTGETKTEKTG"
     gene            complement(1399..>1869)
                     /locus_tag="P5573_08280"
     CDS             complement(1399..>1869)
                     /locus_tag="P5573_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRT28097.1"
                     /translation="RLNVIPIWIPPLRKRKEDIPLLVRYFSEKYSREFGKDVKKISAY
                     ALELLLEYEFPGNVRELENIIERSVALETSNIILPENLVISKQGEGEKKGLPAMGIEL
                     PEEGVMLNETMNEVERSLITKALDRARGSKKKAAELLHISYDSLKHRLEKFGIT"
BASE COUNT          387 a          548 c          436 g          498 t
ORIGIN      
        1 gaccggccag cgcgtaatga agatattcga ggcaatcgag acggcctacg tacagtacac
       61 ccggcgggta cagacggccg agctgaacgt gacggtccgc ggaatcgtcg agaagaatcc
      121 cccttcccgg cacctgggga ggccgaactc ggtgagctac gtgacgcaga cctccgtcaa
      181 acccccgacc ttcgtcttct tcgtccggga gccgaaggcc gtcctccctt cctacgagcg
      241 ctacctcgcc aaccagctcc gcgacgccct cggttttcag tacacgcccc ttcggctgat
      301 atttcgcagg aagaggagat aaatggatgt cggtcagtct tatagggggg aaccaggaga
      361 gtacgtaaca gaaaaggggg cgagagcccc cttttctgtt gttccccggc cgcaagagaa
      421 gagggttatc ctgttttttc ggtttttgtc tcgccggtct tgctgtcgta agtcacctta
      481 ctgccagcct tagtactagt cacttcgact ttaaaataac tcttaccgtc ttcagtataa
      541 ggggtaactg taacggtatt gccagccgaa gctttcacac caccgaagtc tttaagggcg
      601 tcaatagcag cctgatctgt cgccgtaccc tccacatagg tgttgttctg tgcgtagtag
      661 gcctcctctg ccaacctaac gctgttcagg tccgcctgca tcgaagcaat gtagcccctt
      721 tctctgtacg ccgcgaactg cgggatggcg atggccgcca ggataccgat gatcgcgacg
      781 acgatcatca actctaccag ggtgaaacct ttctgtcctt tcgtccttac tttcattact
      841 ttcatcattc tttcctcctt aggtcagttt ttttaagtct ttgattcaaa caaaaacctt
      901 cttctcatcc ccctcctttc ttttccgttt tccactgaaa atcacgttat tgccgtttgc
      961 cattttagca agtctcacgc caagagctgc gtgcagtcct ctcaaccgga aaactccgtc
     1021 tattctttgc agaatcaaga cctttgaatt tgtgatcaaa tcaccttgcc gtcattccgt
     1081 gcttgacacg gaatccagtg ttttcaacaa gttctggatt ccgccccgat tttcatcggg
     1141 gtgacgcgtc ggtcggaatg acatgagggg ccatttttca acgatcttga atcaataaga
     1201 tacatgggcg accaccgcct cctcgttcat ccgccgactc tcccaataat aaaaaaaccg
     1261 gcgcttttca cccatctctg ggtcatcctc accgcttatt tatccaaggt ttagtctgtc
     1321 gccgcggcaa atcgaaccct gccacgccgg agcagcctga ggaagttccg tgcctttctc
     1381 caagaaccga taacggcatc aagtgatgcc gaatttttcg agtcggtgtt tcagagaatc
     1441 gtagcttatg tggagaagct cggccgcctt cttcttcgac ccccgggccc tgtccagggc
     1501 cttcgtgatg agcgaccttt cgacctcgtt catcgtctcg ttcagcatta cgccctcttc
     1561 cggcagctct atccccatgg cgggaaggcc cttcttttct ccctccccct gcttcgatat
     1621 gacaagattc tcgggaagga tgatgttcga cgtctccagg gcgacgcttc tttcgattat
     1681 gttctccaac tccctgacgt ttcccgggaa ttcgtattcc agcaaaagct ccagcgcgta
     1741 ggccgatatc ttcttgacgt ccttgccgaa ttcacgggaa tacttttcgc tgaaatatct
     1801 gacgaggagc gggatgtctt cttttctctt cctcagggga ggtatccata tgggtatgac
     1861 gttcaggcg
//
LOCUS       DAOTMX010000322         1859 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_48646_length_1859_cov_3.355322, whole genome shotgun sequence.
ACCESSION   DAOTMX010000322 DAOTMX010000000
VERSION     DAOTMX010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48646_length_1859_cov_3.355322"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..987)
                     /gene="lpxC"
                     /locus_tag="P5573_08285"
     CDS             complement(58..987)
                     /gene="lpxC"
                     /locus_tag="P5573_08285"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HRT28098.1"
                     /translation="MYLQRTIKSEIRCHSVGLHSGRKVNMTLKPAEADEGIVFVRKDL
                     PGDNRVKADIRNVSDTTLATTLGLNGARVATVEHLLSAFRGVGVDNATVEIDSFEVPI
                     MDGSALPFVNLMKACGTKVQNRGRRFLVIKKEVAVSDGDAEARFVPSPDFRITYRIGY
                     DHPLISDQTYHMVFSHVAYEEDICAARTFGFLRDVEYLQAKGLALGGSLNNAVVLDDE
                     KIINKEGLRFPGEFVKHKVLDAIGDLFLLGMPIVGHFIGNKSGHRLNNALLRKLIESR
                     DAWKIISHFERDNRINDVEDGSFHNIPPISNLA"
     gene            complement(1185..1745)
                     /locus_tag="P5573_08290"
     CDS             complement(1185..1745)
                     /locus_tag="P5573_08290"
                     /inference="COORDINATES: protein motif:HMM:NF014707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HRT28099.1"
                     /translation="MKILLHICCAPCTIYPLRVLREEGMEATGLFFNPNIHPCLEYRK
                     RLETLQGYASMAGLQLFFDDAYPMEEFLRNVSFREDERCRCCYYMRLGRTAKTARNEG
                     FDGFTTTLLYSKYQRHELVREIGESLAERHGVAFLYRDFRVGWKEGVEKAKEIGLYRQ
                     AYCGCIYSEKERYWKGMKAKAPTGAP"
BASE COUNT          410 a          527 c          470 g          452 t
ORIGIN      
        1 attatcttgc agggagtata ctaatactcg gaaagaaata gtaaaagcaa gcgatattta
       61 agcaagatta gaaataggtg ggatgttgtg aaaggaaccg tcttctacgt cgtttattct
      121 gttgtctctc tcaaaatggc tgatgatctt ccaggcgtct ctgctttcta tcaatttacg
      181 cagcagtgcg ttgttcaaac ggtgaccaga tttattaccg ataaaatgac cgacaatcgg
      241 catccccagg aggaagaggt caccgatggc gtcgaggact ttgtgcttca cgaattcgcc
      301 gggaaagcgc aacccctcct tattgatgat cttttcatcg tcgaggacga cggcgttgtt
      361 aagagagccc cccaacgcca atcccttcgc ctgtagatac tcgacgtctc tcaggaaccc
      421 gaaggtcctg gcggcgcaga tatcttcctc ataggcgaca tgggaaaaga ccatgtggta
      481 ggtctggtcg ctgatgagag ggtggtcgta gcctatccgg taggtgatcc gaaagtcggg
      541 agacggaacg aaccttgctt ctgcgtctcc gtcggagacg gctacctcct tctttatgac
      601 gaggaacctc ctcccccggt tttggacctt ggttccacag gccttcatca gattaacaaa
      661 ggggagggcg ctcccgtcca tgatgggaac ctcgaaggag tcgatttcaa ccgtagcatt
      721 gtcgaccccg acccctctga aggcggaaag gagatgttcg accgtcgcta ctctggcgcc
      781 gttcaggccg agcgtcgtgg ccaatgtcgt atcggaaaca ttccggatat cggccttgac
      841 cctgttgtcc ccgggcaggt ccttcctcac aaaaacgatc ccttcgtccg cctccgccgg
      901 tttcaacgtc atgttgactt tccggcccga atgcagccca acactgtgac acctgatttc
      961 gctcttgatc gtcctctgga gatacatgcc ctttggcctt acttttttct gttaattaca
     1021 ggcaaaatcc ataccaccca cggttcccgc ggggccacag agacctgctt gtaactatgg
     1081 gataataagc gcgtttttca aatatcccgt cctggttgtt cagccggaag accgctgccc
     1141 gcccggcctg tgttcgtcac gacacatccc gtaacatctg tgcatcaagg ggcgcccgtc
     1201 ggggctttcg ccttcatacc tttccagtac cgctctttct cgctgtagat gcacccgcaa
     1261 taggcctgcc tatacaggcc gatttctttc gccttttcaa ctccttcttt ccaaccgacg
     1321 cgaaagtccc ggtagaggaa agctactccg tgcctctccg cgaggctctc accgatttct
     1381 ctcaccagtt catggcgctg gtacttgctg tagaggagcg tcgtcgtaaa accgtcgaac
     1441 ccttcattcc gcgccgtttt cgcagtgcgg ccaagacgca tgtagtaaca gcacctacaa
     1501 cgctcgtctt ctcggaagga tacattgcga agaaattcct ccatgggata ggcgtcgtcg
     1561 aaaaaaagct gcagaccagc catcgaagca tatccctgaa gagtctcgag cctcttgcgg
     1621 tactcgagac agggatggat gttggggttg aaaaaaaggc cggtggcttc catcccctct
     1681 tctctcaaga ccctgagagg atagatggtg caaggggcac agcagatgtg gagcaatatt
     1741 ttcataacgg gaacggctca gaacaattct ccttggcttc tcgcccacgc cctgccgaaa
     1801 tggtcgtagg cgagcttggt cgctacgcgc cccctcgcgg ttcggtgcag gtaaccctc
//
LOCUS       DAOTMX010000323         1850 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_48981_length_1850_cov_3.097493, whole genome shotgun sequence.
ACCESSION   DAOTMX010000323 DAOTMX010000000
VERSION     DAOTMX010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48981_length_1850_cov_3.097493"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="P5573_08295"
     CDS             <1..733
                     /locus_tag="P5573_08295"
                     /inference="COORDINATES: protein motif:HMM:NF028171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HRT28100.1"
                     /translation="LGPEFSDGEVEAAVKKYGAHYRRYSDFDELCAATALLLRQGNVV
                     GWFQGRMEWGPRALGNRSILADARNPEMQKKLNLKIKFREGFRPFAPAVLAEDASDYF
                     DIDKDSPYMLLVADVRTDRRNELPEDYHSLPWRDKLYYLRSDVPAVTHIDCSARLQTV
                     HRESNPRFWQLIKRFKEETGYAVLVNTSFNVRGEPVVCTPEDAYRCFMRTEMDYLVIG
                     PYLFAKKEQPVWEEEKDWREEFGVD"
     gene            791..1159
                     /locus_tag="P5573_08300"
     CDS             791..1159
                     /locus_tag="P5573_08300"
                     /inference="COORDINATES: protein motif:HMM:NF039992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SxtJ family membrane protein"
                     /protein_id="HRT28101.1"
                     /translation="MKDEEYKTLETMGILAFFCLVLGIACDLYVLIYASACLLFIALF
                     VKGVAAAIARLWMRFAFALGALNTKIILTVTFFLILTPIAVLYRWKHGDFLSIRETPV
                     KTLWHERNHEYRAEDLEKQW"
     gene            1197..>1850
                     /locus_tag="P5573_08305"
     CDS             1197..>1850
                     /locus_tag="P5573_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRT28102.1"
                     /translation="MKRILSRESFIRLLLVVVGIVAALLVGEFGLRAYYAINGSDGKY
                     YVWPPNMQKVFRPTPGTMPGVSGPTVFRVNSDGVRGDELTDDFEYRILAVGGSTTECL
                     FLDQEVSWPYALQKKLNDLKGPKVWVGNVGKAGLSTREHYMHMKYLLPQFPKVDAVVI
                     LAGFNDLIRRLIEDEKYDPLFLQHYDYGEPRLIRGAFWETPYYKGKYRVRTGYYDETA
                     "
BASE COUNT          401 a          495 c          558 g          396 t
ORIGIN      
        1 tcttgggcct gagttttcgg acggcgaggt agaggcggcg gtcaagaaat acggggctca
       61 ctacaggcgc tattccgatt tcgacgagct ctgcgcggcg acggcgctcc ttctgcggca
      121 gggcaatgtc gtcgggtggt ttcagggtag gatggagtgg gggccgaggg ctctggggaa
      181 ccggagcatc ctggccgacg caagaaaccc ggagatgcag aagaagttga acctgaagat
      241 caagttccgg gaggggtttc gccccttcgc tcccgcggtc cttgcggagg acgcgtccga
      301 ctatttcgac atcgacaagg attcgcctta catgcttttg gtggccgacg tgaggaccga
      361 ccggcgaaat gaactgcctg aagattatca ttcccttccc tggcgagaca agctctacta
      421 cctgcgctcc gacgttcctg ccgtcacgca catcgactgc tccgccaggc tgcagacggt
      481 ccaccgtgag tcgaaccccc ggttctggca attgataaag agattcaagg aagaaacagg
      541 ctatgcggtg ctcgtcaaca cgagtttcaa cgtccgggga gaacccgttg tctgcacacc
      601 ggaggatgcg tacaggtgtt tcatgcggac ggagatggat tacctcgtca tcggtcccta
      661 tctcttcgcg aagaaggaac agccggtatg ggaagaagag aaggactggc gtgaagaatt
      721 cggggtcgat tgaccgcttg tttgtgatgt ttgcgacgtt tggacggggg cggaagatat
      781 gcgtgcaacg atgaaagacg aagaatacaa gaccttagag acgatgggta tcctggcctt
      841 tttctgcctg gtgctcggca tcgcctgtga cctctacgta ctcatctatg cctccgcctg
      901 cctcctcttc atcgctctct ttgtcaaagg tgtcgccgcg gctatcgccc ggttgtggat
      961 gaggtttgcc ttcgccctcg gcgctctcaa cacgaagata atcctgaccg tcaccttctt
     1021 tttgattctc acgcccatcg ccgtcctgta ccggtggaag catggagatt tcttgagtat
     1081 ccgggaaaca ccggtgaaaa cactatggca tgaaaggaac catgaatacc gggcggaaga
     1141 cctcgagaag cagtggtgag caatgatcgc gaggggcgag aggaggaaag ccgtatttga
     1201 aaagaattct aagccgcgag agttttatcc gtctcctgct tgtagtcgtc ggcatcgtcg
     1261 cggcgctcct ggtcggtgaa ttcggcctcc gcgcctacta tgccataaac ggcagtgacg
     1321 gcaagtacta cgtctggccc ccgaacatgc agaaggtctt ccgacccacc ccggggacta
     1381 tgcccggggt aagcggcccc actgtgttcc gggtcaattc cgacggtgta aggggggacg
     1441 aactcacaga tgattttgag tacaggatcc ttgccgtcgg gggcagcact acggaatgcc
     1501 tcttcctcga ccaagaggtg tcgtggccct atgccctgca gaaaaagctg aacgacctca
     1561 agggacccaa ggtctgggtg gggaatgtgg gtaaggcggg gctgagcacg agggagcatt
     1621 acatgcacat gaagtatctt ttgccccagt ttcccaaggt cgacgccgtg gtcatccttg
     1681 ccgggttcaa cgacctaatc cggcggctga tagaagacga aaaatacgac ccccttttct
     1741 tgcagcacta cgactatggg gagcctcgcc tcatcagggg tgccttttgg gagacgccct
     1801 actacaaggg taagtacaga gtccgcaccg gttattacga cgagacggcc
//
LOCUS       DAOTMX010000324         1841 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_49338_length_1841_cov_2.975364, whole genome shotgun sequence.
ACCESSION   DAOTMX010000324 DAOTMX010000000
VERSION     DAOTMX010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49338_length_1841_cov_2.975364"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="P5573_08310"
     CDS             <1..514
                     /locus_tag="P5573_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HRT28103.1"
                     /translation="AERPKGAAGSVVVSSIEGTRPILIELQALVSPTGFGMPRRTSIG
                     VDYNRVSLLVAVMEKVCGLHLGNHDIFLNVAGGVRVDEPGADLGIVSSVASSFLDRPI
                     DPGTVVFGEVGLTGEVRGISQMDVRVKEAERMGFSRCIMPRTTSRELPGTKLDLVRVK
                     NLRELLEHLF"
     gene            526..1224
                     /gene="phoB"
                     /locus_tag="P5573_08315"
     CDS             526..1224
                     /gene="phoB"
                     /locus_tag="P5573_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HRT28104.1"
                     /translation="MKILVVDDERDIVELLSYNLEKEGFTVIKAYDGEEALKLVKAKK
                     PDLVILDLMLPGIQGIEVCKSVRGNPETATLPVIMLTAKGEEVDRVVGLEMGADDYVT
                     KPFSVREVVARVRAVLRRTEAEKQPERETFSYGGLEIDYGSHEVTFQGKKVELSPTEW
                     KLLVFFARHPGRVYTRDQILDHVWGDSAFVTPRTVDVHIRRLRAQIEKDTDNPRFILT
                     VRGAGYKFAEVRSL"
     gene            1261..>1841
                     /locus_tag="P5573_08320"
     CDS             1261..>1841
                     /locus_tag="P5573_08320"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HRT28105.1"
                     /translation="MDKSRKAIGGMFDRIAPSYDLLNRVLSLYLDALWRKKALKHLAI
                     KDGEQVLDIATGTGDLALEALKAGKACRVCGLDFSEEMMRIAAAKRDRLGLRNRYLLV
                     GGDALRMPFKEKSFDSAMVAFGVRNMPNVEGFLREVHRILRGGGRLAVLELSLPGNPV
                     IRECYLLYFKKFLPHIGNLLSGRSTAYHYLRDSV"
BASE COUNT          384 a          483 c          588 g          386 t
ORIGIN      
        1 cgccgagagg ccgaaggggg ccgccgggtc ggtggtcgtc tcgagcatag aagggacgag
       61 gccgatcctg atcgaactcc aggccctcgt cagccccacc ggtttcggga tgccccgaag
      121 gacgtctatc ggcgtggatt acaaccgggt gtcgctgctc gtggccgtga tggaaaaggt
      181 ctgcggcctt caccttggca atcacgatat cttcctgaac gtggcgggcg gagtccgcgt
      241 ggacgagccg ggggccgacc tgggaatcgt ctcctcggta gcgtcgagtt ttctcgaccg
      301 cccgatagac cccgggactg ttgtgtttgg tgaggtgggg ttgaccggag aagtaagggg
      361 gatcagccag atggacgtca gggtcaaaga ggcggaacgc atgggattca gccgctgcat
      421 catgccgcgg actacttctc gtgagcttcc ggggactaag ctcgacctcg tcagggtaaa
      481 aaatctaagg gaactgctcg aacatctttt ttaggaaggt ccgccatgaa gatactcgtt
      541 gtggacgacg agagagacat cgtcgagcta ctctcctaca acttagagaa ggagggtttt
      601 acggtcatca aggcctacga cggggaagag gcgctcaaac tggtgaaggc aaagaaaccg
      661 gaccttgtga tcctcgacct catgcttccc ggcatccaag gaatcgaggt ctgcaaatcc
      721 gtccggggga atcccgaaac ggcgacgctc cccgtcatca tgctgacggc caagggcgag
      781 gaggtcgaca gggtcgtcgg gctggaaatg ggggccgatg attatgtgac caagcctttc
      841 agcgtgaggg aggtcgttgc cagggttcgg gcggttctga ggaggacaga ggcggagaag
      901 cagcccgaga gggagacctt ttcgtacggt gggctcgaga tcgattacgg ttctcacgag
      961 gtgacgtttc aggggaaaaa ggtcgaactg agtccaacgg aatggaagct ccttgttttc
     1021 tttgcccgac acccgggcag ggtctacaca agagaccaga ttctcgacca tgtctggggc
     1081 gattccgcct ttgtaacgcc ccgcacggtg gacgttcata tccggcgtct gcgggcgcag
     1141 atcgagaaag acaccgacaa cccccgtttc atcctcaccg tcaggggtgc ggggtataag
     1201 tttgccgaag tccgatcact ctgatttcta ttcctttccg cttcttattt gataatcgtc
     1261 atggataaat cgcgtaaggc aatcggcggg atgttcgaca gaatcgcccc ctcttacgac
     1321 cttctcaata gggtgttgtc gctttacctc gatgccctgt ggcggaagaa ggccctcaag
     1381 catctggcaa tcaaggacgg tgagcaggtg ctggacatcg ccacggggac gggagacctg
     1441 gcgctggagg ccctgaaggc ggggaaagcc tgccgcgtct gcggcctcga cttctcggaa
     1501 gagatgatgc ggattgctgc ggccaagcgg gacaggctgg ggctacggaa ccggtatctc
     1561 cttgtcggcg gcgacgccct gcggatgccc ttcaaggaaa agagcttcga cagtgctatg
     1621 gtcgccttcg gggttagaaa catgcccaat gtcgaaggtt ttttaagaga agtgcatcgt
     1681 atcttgcgag gcggggggag gttggccgtc ctagaacttt cccttcccgg aaatcctgtc
     1741 atccgggagt gttaccttct gtatttcaag aagttcctgc cccatatcgg taatctgctg
     1801 tccgggcgct cgacagccta ccattacctg agggattccg t
//
LOCUS       DAOTMX010000325         1834 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_49602_length_1834_cov_4.205734, whole genome shotgun sequence.
ACCESSION   DAOTMX010000325 DAOTMX010000000
VERSION     DAOTMX010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49602_length_1834_cov_4.205734"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="P5573_08325"
     CDS             <1..580
                     /locus_tag="P5573_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HRT28106.1"
                     /translation="IGCFGRIKALCEHIHLPVQSGSDRILNRMNRQYTRSDYIEMVGK
                     LRAAAPGISITSDMIVGYPGETEDDFEDTLSLMETVRFDGLFSFKYSDRGGTLAACQR
                     DKVAEEVKNVRLTRLQSLQDTHTLEINKALEGRTEEVLVEGISKNANQDVSGRTRTNK
                     IVNFRGSVELIGKTVNVTIVKTYRHSLRGEMI"
     gene            596..1096
                     /locus_tag="P5573_08330"
     CDS             596..1096
                     /locus_tag="P5573_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HRT28107.1"
                     /translation="MLIQMKVAGLTIDPLTNTPIVILKDLAESKALPIWIGLFEASAI
                     ATELEKIEFSRPMTHDLLKQVIKGLDVSVTMIEINDLRDNTFFAVIHLLKEGTSIKID
                     SRPSDAIALALRADAPIFVDEKVIEKSRNIDFDKKYVNADDLKKDKLKEFLENLSPDA
                     FGKYKM"
     gene            1433..>1834
                     /locus_tag="P5573_08335"
     CDS             1433..>1834
                     /locus_tag="P5573_08335"
                     /inference="COORDINATES: protein motif:HMM:NF014898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HRT28108.1"
                     /translation="MDDLRRAVEDFRRHLEEEKAFSAYTQKSYMTDLQQFVDFMERPG
                     TAGAAGVESLTMIEPSDVRGFLVDLYKRKVRKSSISRKVSALRSFFKFLVREGKIRIN
                     PAEAVQSPKIERYLPTFLQVDEMFTLLNTEFN"
BASE COUNT          495 a          397 c          515 g          427 t
ORIGIN      
        1 aatcggctgc ttcggccgga taaaggcctt gtgcgagcac atccatcttc ccgtgcagtc
       61 gggctccgac aggattttaa accggatgaa caggcaatac acccggagcg actacattga
      121 aatggtcgga aagctccggg cagcggcacc gggtatcagt atcacatcgg acatgatcgt
      181 cggatacccc ggggagacgg aagacgattt cgaggatacg ctgtctttga tggaaaccgt
      241 ccggtttgac gggctttttt cctttaagta ttccgaccgc ggcggtactt tggcggcctg
      301 tcagcgggac aaagtggccg aggaagtaaa gaacgtgaga ttgacgagac ttcagtcttt
      361 gcaggacact cacacgctcg agataaacaa ggccttggaa gggagaacgg aggaagtcct
      421 cgtagaaggg atcagcaaga acgcaaacca agacgtcagc ggtaggacga ggacgaacaa
      481 gatagtcaat tttcgggggt cggtagagtt gattggcaag acggttaatg ttacgatagt
      541 gaaaacctac aggcactccc tgagaggtga aatgatctga aaaggaggtt ggggaatgct
      601 tatccagatg aaagtggcag gattgacgat agatcctctt accaatacgc cgatcgtcat
      661 cttgaaagac ctggccgaga gcaaggcctt gcccatatgg atagggcttt tcgaggccag
      721 cgcgattgcg acggagttgg agaagatcga attttcccgg cccatgacgc atgacctcct
      781 caaacaggtg ataaagggcc tcgacgtgag cgtgactatg attgagataa atgacctcag
      841 ggacaacact ttctttgccg tcattcacct actgaaggaa gggacgtcca tcaaaataga
      901 ctcgagaccc agcgatgcca tcgctcttgc cctaagagcg gatgccccta tatttgtcga
      961 tgaaaaggtg atcgagaagt cgcggaacat cgattttgac aagaagtacg tcaatgccga
     1021 tgacctcaag aaggataaat tgaaggagtt tctcgaaaac ctttcacctg acgccttcgg
     1081 aaaatacaag atgtaggctt tggtgttttt atgaaagaag aggtccggcg cctttatctc
     1141 aaatcagggg atcaagtccg gcacctgagg tttccggaat gggggtttgg cgtggtcata
     1201 gaggaatgga actcacagat tctcgggggt atttcttacg taaagataac gttcagagat
     1261 ggtcaaacga ggatatttga caataatttc gccaattcct gctgttgtta ttacgcgggg
     1321 ataaggcgat gctgaatgat tgaccttccg ccacgaagcg cttgaaatag cttgattttc
     1381 aaactatttt tggtgtaact tcgtggctat tatttttaat cggggtttgc cggtggatga
     1441 cctgagaagg gccgtcgagg atttcagaag acatcttgaa gaggagaagg ccttttctgc
     1501 gtacacacag aaaagttaca tgacggacct tcaacaattt gtggatttta tggagcgacc
     1561 gggtacggcc ggcgctgcag gggttgaatc gctgaccatg atagagcctt ctgacgtaag
     1621 aggctttctc gtcgatcttt acaaaaggaa agtgcgaaag agcagcatat cgcgcaaggt
     1681 ctcggccctt aggtcatttt tcaagttcct tgtcagggaa gggaaaatca ggataaaccc
     1741 ggcggaggcc gttcaatcgc cgaagataga gcgctatctg cccacgtttc ttcaggtcga
     1801 cgagatgttc acgttgctca acacggagtt caac
//
LOCUS       DAOTMX010000326         1813 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_50438_length_1813_cov_3.345848, whole genome shotgun sequence.
ACCESSION   DAOTMX010000326 DAOTMX010000000
VERSION     DAOTMX010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50438_length_1813_cov_3.345848"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1311
                     /locus_tag="P5573_08340"
     CDS             <1..1311
                     /locus_tag="P5573_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT28109.1"
                     /translation="GLLGRRSEYESLYGEYRGLGARLAELQALRKKKVEREDLVRYQL
                     REIHEARVKPGEDKALLEEKAVLSNAQRLMEYAARSYEVLYGGEKSVLGGLKSALQDI
                     REIKAIDSSLRVSPEELDSLFFQLEDAAVALREYAGRVVFDPQRLSEVDERLALLAGL
                     KRKYGGSLDAVLKTGEALESELGGMSAVEEEISGVEKEVSALKERLVEQARGLTEERR
                     RAAGGLKAAVEREIASLRMAEARFEALVSTPSAGDGSGEPVLTAKGQDCVEFYISTNV
                     GEDLKPLNRVASGGELSRIILALKKVLAKTGSVGTIVFDEVDSGIGGAVAEVVGEKLK
                     EVAEHHQVVCITHLPQIASFGDSHYLVSKSVYGTRTNSRVRRLSESERVEEVARMLGG
                     VDVTEKASEHAREMLKKAKRAKSDEAVGRKRGSRRGLSRGIEEC"
     gene            1305..1763
                     /locus_tag="P5573_08345"
     CDS             1305..1763
                     /locus_tag="P5573_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HRT28110.1"
                     /translation="MLRKARVGDVKTIHRMIKISSDREEMLPRSLMDIYGNLRDFFVY
                     VDEGSDAVIGICAMSIIWENLAEVRSLYVEEGYRERGIGRKLVEACISEAITLQLYRV
                     FTLTYKQAFFEKLGFRPVERKSLSEKIWSDCFKCPKYPDYCDEVAMVIEL"
BASE COUNT          432 a          431 c          615 g          335 t
ORIGIN      
        1 ggactgctag gccgccgctc cgagtatgaa tccctttacg gtgaatatcg ggggctcggg
       61 gcgcgactgg cggagcttca ggcattgaga aagaagaagg tcgagcggga agacctggtc
      121 cggtatcagc tccgcgagat acacgaggcc agggtcaaac cgggcgaaga caaggccctg
      181 ctggaagaga aggccgtctt gagcaatgcg caaagactga tggagtatgc cgcacggtct
      241 tatgaggtac tctacggggg agagaaatcc gttctgggcg gactcaaatc ggctctccag
      301 gatatccggg agatcaaggc catagactcc tccttgaggg tctctccgga agagttggat
      361 tcccttttct tccagctcga ggacgcggca gtcgcgttga gagaatacgc cgggagggtc
      421 gtcttcgacc cgcagagact ctccgaggtg gatgagcggc ttgcgctgct ggccggccta
      481 aagcggaaat acggcgggtc cctcgacgcg gtgttgaaaa ccggcgaggc cctggagtcg
      541 gaacttggcg gaatgagtgc cgtcgaggaa gagatttcag gggtggagaa agaggtgtcc
      601 gcgctcaaag aacggctcgt cgagcaggcc cgggggctca cggaggagag acgacgggcc
      661 gccggagggc tcaaggcggc cgtcgaacgg gaaatcgcgt cgctcaggat ggccgaggcc
      721 cgtttcgagg ccctggtgtc taccccgtcg gcgggggacg gaagcggcga gccggtgctg
      781 acggcaaaag gtcaggactg tgtggaattt tatatctcca cgaatgtggg tgaggacctc
      841 aaacctttaa accgcgtcgc ctcggggggt gaactctcca ggatcatact ggccctgaaa
      901 aaggtcctag caaagacagg gtccgtcggt acgatagttt ttgatgaagt cgacagcggc
      961 atagggggag ccgtagccga ggtggtgggt gagaaactga aggaagtggc agagcaccac
     1021 caagtcgtct gcatcaccca cctgccgcag atagcctcct tcggcgacag ccattatctg
     1081 gtgtcgaaat ccgtttacgg cacgaggacg aacagccgcg ttcgccgtct ttctgagagc
     1141 gagcgtgtcg aagaggtcgc gaggatgctg ggaggcgtgg atgtgacgga aaaggcatcg
     1201 gaacatgccc gggagatgtt gaagaaggcg aagagggcga aaagcgatga ggcagtcggc
     1261 cgcaaacggg gcagccgacg aggtttaagt cgagggatcg aggaatgtta aggaaggcga
     1321 gggtcggcga cgtcaagacc atccaccgga tgataaagat ttcttcggac cgggaagaga
     1381 tgcttccccg ttcgctcatg gatatctacg gaaatctcag ggatttcttt gtgtacgtcg
     1441 acgagggaag cgatgccgtc atcggcatct gtgccatgag tatcatctgg gagaacctgg
     1501 ctgaggtaag gtctctttac gtggaagaag gttacagaga aaggggaatc gggagaaagc
     1561 tcgtagaggc ctgtatctcc gaggccatca cgttgcaact ttaccgcgtc ttcaccctga
     1621 cgtacaagca ggcctttttc gagaaactgg ggttccgacc cgtcgagagg aagtcacttt
     1681 cggagaagat ctggtcggat tgcttcaaat gccccaaata tcccgactac tgcgatgaag
     1741 tggcaatggt catcgagctt taagctgaat ggtgaagacg aaaggcgaaa ggatagaatg
     1801 gaaatgaagc cac
//
LOCUS       DAOTMX010000327         1812 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_50481_length_1812_cov_3.680706, whole genome shotgun sequence.
ACCESSION   DAOTMX010000327 DAOTMX010000000
VERSION     DAOTMX010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50481_length_1812_cov_3.680706"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="P5573_08350"
     CDS             complement(<1..550)
                     /locus_tag="P5573_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HRT28111.1"
                     /translation="MSRGFEAAALFISLVLALTGCMVQQSTYQKKAAEADRLGNEVIA
                     LQQRNKVLAEENELLRQQVQNRSEEKKQAGVTAEAGAPVGPPEKEEPKVEEEKVAEPR
                     KEKAAESRTVTAAPKPPEKKAADVKSMRLKVLSGNGRMSSAKKMSAKLTKLGYKVEVV
                     GMATTPDYATDTIYYKSGFEKQA"
     gene            complement(830..1093)
                     /locus_tag="P5573_08355"
     CDS             complement(830..1093)
                     /locus_tag="P5573_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT28112.1"
                     /translation="MDRLEKLRHIKQAALYVEPQLMGSDFDRVRAAEEEWLSLVDQLY
                     EGNGHIITERQYQAARTQFEYFMVLLEMTIAHRLREAREESEK"
     gene            complement(1126..>1812)
                     /locus_tag="P5573_08360"
     CDS             complement(1126..>1812)
                     /locus_tag="P5573_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT28113.1"
                     /translation="CFFMAASVNAGAAEEKAHLLKDNALSIKAGYHLYSDSDFMDFWN
                     LDRIDFNGFIGEVAYERRLYKNLWLEVALGFYQSGEEYTGARLAGDYMDLTISNAYFS
                     PTLKAVVPLSDFFIVHAGVGPDYCYTQSRLRYRAGGLIYDRDENFSSFGAHGLLGIEF
                     YVMRRPADFGFWDAPVGIFFEYKYTWLPVKDFDEGLINVLNDSYGTSYGSHEADVGGS
                     TFLGGIRWHF"
BASE COUNT          348 a          562 c          471 g          431 t
ORIGIN      
        1 ccgcctgctt ctcgaaaccg gatttgtaat agatcgtgtc ggttgcgtaa tccggcgtcg
       61 tcgccatgcc cacaacctcc accttgtagc cgagtttcgt cagtttagcc gacatcttct
      121 tggccgagga catcctgccg tttccgctca gcaccttgag gcgcatggat tttacgtctg
      181 cggctttctt ttcgggcggc ttcggggccg ccgtcaccgt gcggctttcc gcagccttct
      241 ctttcctcgg ctccgcgacc ttttcctcct ccaccttcgg ttcctctttc tccggcgggc
      301 cgaccggagc tcccgcttcg gccgtcacgc ctgcctgctt cttttcctca cttcgattct
      361 gaacctgctg cctgagcagt tcgttttcct ccgcgagcac cttgttgcgc tgctgaagag
      421 cgatcacttc gttgccgaga cgatccgctt ccgccgcctt tttctggtag gtgctttgct
      481 ggaccatgca ccccgtcagg gcaaggacaa gactgatgaa aagggccgca gcttcgaaac
      541 ctctcgacat gagtcgcctc cttcttattg atcaccttcc tggcaggggc gtggacaccc
      601 ttatccgata cgacagccaa aaactcgtac agttcaatcc acagctctgt gagtaattct
      661 gtggacagct ttgtggacag tatccgtaag ttcgcttaat cgcttcgact tgaccgggtt
      721 gccctcgatt taggcaaaat aaaatgagta atttcactga gttgaattta gaccatcctt
      781 cggggccatt cgtgaaaccc attccctcaa cgtgcaacgg cgctgcccgt catttttcgc
      841 tctcttcccg ggcctcccgc aggcgatggg cgatcgtcat ctccagaagg accatgaagt
      901 actcgaactg ggtcctggcc gcctggtact gccgctccgt gatgatatgg ccgttgccct
      961 cgtagagctg atccacgagg ctaagccatt cctcttcggc cgcccggacg cggtcgaagt
     1021 ccgaacccat gagctggggc tcgacataga gggcggcctg tttgatgtgc ctgagctttt
     1081 ccagcctatc catgaatggt gaccgcctgc agaggtttcc tcccgtcaaa aatgccagcg
     1141 gattccgcct aaaaacgtgc tgccgccgac atcggcctcg tgactcccgt aggacgtacc
     1201 gtaagagtcg ttcagaacgt tgatgagccc ttcatcgaaa tcctttaccg gaagccacgt
     1261 atatttatac tcgaaaaaga tccccaccgg cgcgtcccag aacccgaaat ccgcgggcct
     1321 cctcatgacg taaaactcga tccccagcag gccgtgggcg ccgaagctgc tgaagttctc
     1381 gtccctgtcg tagatgagcc cccccgcccg gtacctgagc ctgctctggg tgtagcaata
     1441 atcgggaccc acccctgcat ggacgatgaa gaaatccgag aggggtacga cggctttcag
     1501 ggtcggggaa aaataggcgt tgctgatcgt caaatccatg taatcgccgg cgagacgggc
     1561 ccctgtgtat tcctcgccgg actggtagaa gcccagggca acctccagcc agaggttctt
     1621 gtacagacgt ctctcgtagg caacctcccc gatgaagccg ttgaaatcga tccggtcgag
     1681 attccagaag tccatgaagt cactgtcgga atagaggtga tatcccgcct tgatggaaag
     1741 ggcgttgtct ttcaggagat gggccttttc ctctgcggcg cccgcgttca ccgacgcggc
     1801 catgaagaaa ca
//
LOCUS       DAOTMX010000328         1812 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_50487_length_1812_cov_3.303358, whole genome shotgun sequence.
ACCESSION   DAOTMX010000328 DAOTMX010000000
VERSION     DAOTMX010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50487_length_1812_cov_3.303358"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="P5573_08365"
     CDS             complement(<1..478)
                     /locus_tag="P5573_08365"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HRT28114.1"
                     /translation="MRWVLFSDLDGSLLNYDDYSFTEARPALEKIKGFGIPLVLTTSK
                     TRKEVEAILAEMDLREPFIVENGAALFFPARYRGWDLAGGVRFQSYVVFKLGISYADI
                     RAFMKRTASGFGIKGFGDWSVEVIANITGLPPEKVKLAKEREFTEPFLPGDEKEMEE"
     gene            794..>1812
                     /locus_tag="P5573_08370"
     CDS             794..>1812
                     /locus_tag="P5573_08370"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRT28115.1"
                     /translation="MNLLLIFIIGIGLVAFLSLFYSALSRRRVEKRLNNVLESSPAPT
                     FVIGRDHRVIYWNRAMEKLTGVKAEDVIGTRGHWRPFYNTERPCMADLAMDGAAEADS
                     RRHTGEGENPAGQDRVEVKTDFFPNMGAGGRWIRFTAAVIRDSRGEIDGAIEVLEDVT
                     KEKEAEENLVNLKKLESLATFAGGVARDFDTLISTILRGIFLAKLSTPEEDETMEQAL
                     TAAEKASLLAKELSFKLVTYAKGGHPLRKTESLIPVLQEALQSIPPESGVEFSASIPE
                     DLWPVRIDSTQIRQVFESLIMNAVEAMPDGGKLELRAENTVIRGGNSTDIKPGKYIKV
                     SLRDTG"
BASE COUNT          426 a          500 c          519 g          367 t
ORIGIN      
        1 gctcttccat ctccttttca tcgccgggca gaaagggctc cgtgaactcc ctttccttcg
       61 ccaacttgac cttttccggt gggaggccgg tgatattcgc tatcacctcg accgaccaat
      121 ccccgaatcc cttgataccg aaaccggacg ccgttctctt catgaaagcc ctgatatctg
      181 cgtaggaaat gccgagttta aacacgacat aagactgaaa tcggaccccg cctgcaaggt
      241 cccagccccg gtatcgggcg ggaaagaaca gggcagcgcc gttctccacg ataaagggct
      301 ccctcaaatc catttcagcc aggatggcct cgacctcctt ccgagtcttg ctcgtcgtaa
      361 ggaccagagg aattccgaat ccctttattt tctccagggc gggtcgcgct tctgtgaagg
      421 aatagtcgtc ataattcaga agacttccgt cgaggtcgct gaacaggacc caccgcatac
      481 gcctccccat acgctgaact cggttggatc gacttcgaag gtggtttcaa tcctaagaca
      541 acatccgtgt tggattatga tagcggactt tcatgggttt gtctatcgat tggtttgtat
      601 acggaacgcg gagaggcggt ttccacacgg cctgaccgct ttcagaaaaa tgcctccgaa
      661 gggccgactg tttgctggat atagttgctc tttatggccg gtgtgttgta gagtaactta
      721 attattgagc gactaagcga ctatcccaaa cgagcgttcc gcgggcttcg ggacgggaaa
      781 acgagcactg gccatgaatc ttcttctgat atttatcata ggcatcggtc ttgtcgcgtt
      841 tttgtcgctt ttctactcgg cactgtcgag gagaagggta gagaagagat tgaacaacgt
      901 cctcgagagc tcccccgctc ccacttttgt aatcggtaga gaccacaggg tcatctactg
      961 gaaccgggcg atggagaagc tgacgggggt caaggcagag gatgtcatcg gcaccagggg
     1021 acactggcgg cctttttaca atacggaaag gccctgcatg gcggacctgg ccatggacgg
     1081 agcagcggag gctgattccc gccggcacac gggagaaggt gagaatccgg caggacagga
     1141 ccgagtcgag gtaaagacgg acttcttccc gaacatggga gccggcggcc ggtggatacg
     1201 gttcacggcc gcggtgatcc gggacagccg cggcgaaata gacggggcga tcgaggtcct
     1261 ggaagacgta acgaaggaga aagaggcgga agagaacctg gtaaacctga aaaaactgga
     1321 atccctcgcc accttcgcgg gcggcgtagc ccgggatttc gataccctga tctctaccat
     1381 cctgcgcggc atcttcctcg cgaagctttc cacgcccgag gaagacgaaa ccatggagca
     1441 ggcactgacg gccgcggaaa aggccagtct actcgccaag gagctctcct tcaagttggt
     1501 gacctatgca aaaggcggac accccctgag aaagacggaa agcctcatcc ccgtccttca
     1561 ggaggccctt cagagcatcc cacccgaatc gggagtagaa ttcagcgctt ccatcccgga
     1621 ggatctgtgg cctgtccgga ttgacagcac ccagatcagg caggtattcg aaagcctgat
     1681 catgaatgcc gtcgaggcca tgcccgacgg ggggaagttg gaactccggg ccgagaacac
     1741 ggtgatacgg ggaggcaact ccaccgatat caagccgggg aaatacatca aggtctccct
     1801 gagggacacg gg
//
LOCUS       DAOTMX010000329         1796 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_51099_length_1796_cov_3.430213, whole genome shotgun sequence.
ACCESSION   DAOTMX010000329 DAOTMX010000000
VERSION     DAOTMX010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51099_length_1796_cov_3.430213"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /gene="rplA"
                     /locus_tag="P5573_08375"
     CDS             <1..435
                     /gene="rplA"
                     /locus_tag="P5573_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HRT28116.1"
                     /translation="GADYTGSDELIEKIKDGWLEFDRVIATPDMMGSVGKLGKILGPR
                     GLMPNPKVGTVTFDVGRAVKELKAGKVEFRVDKAGIVHCPVGKVSFGPEKICENILAL
                     VEAIMKLKPASSKGTYLKGISLSTTMGPGLKVDPADVRNLLK"
     gene            670..1185
                     /gene="rplJ"
                     /locus_tag="P5573_08380"
     CDS             670..1185
                     /gene="rplJ"
                     /locus_tag="P5573_08380"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HRT28117.1"
                     /translation="MDRKTKEAMVAELHEKLKDVKLAVLADYSGMTVAKMEALRSGLR
                     KTGAEMRVIKNTLLRIASKDTDFAALDRYLTGPVAVILNYGDIVESTKVLVDFAKKNA
                     ELEVKAGFMGGDVLSREQINTLAELPSREVLLGKLLSVMVAAQTGLVRVLSGVPRSLV
                     QVLDAYRMKMV"
     gene            1213..1605
                     /gene="rplL"
                     /locus_tag="P5573_08385"
     CDS             1213..1605
                     /gene="rplL"
                     /locus_tag="P5573_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HRT28118.1"
                     /translation="MTEVKVTKEDVISFIENMTVLELSELVKELEKRLGVTAAAPVAV
                     AAVAAGGAAPAPAEEEKTEFDAILTGFGDQKIQVIKVVRAITGLGLKEAKDLVEGVPK
                     PIKEGIPKAEAEDIKKKIEEVGGTVEIK"
BASE COUNT          455 a          398 c          591 g          352 t
ORIGIN      
        1 ggggcggatt atacgggctc agacgaactg atcgaaaaga tcaaggacgg ctggctcgaa
       61 ttcgaccggg tgatcgccac accggacatg atgggaagtg tgggcaaatt agggaagatc
      121 ctcgggccgc ggggactgat gccgaacccg aaggtaggga cggttacttt cgacgtcggc
      181 agggcggtga aggaactcaa ggcgggcaag gtcgagtttc gcgtcgacaa ggcgggtatc
      241 gtgcactgcc ccgtgggaaa ggtttctttc ggaccggaaa agatatgcga gaacatcctg
      301 gcgctggtcg aagcaatcat gaagctcaaa ccggcctcga gcaagggtac gtacctaaag
      361 ggtatctccc tttcgacgac catgggtccc ggcctcaagg tggacccggc cgatgtcagg
      421 aatttattga aatgatcgat cgttcggcgc gcgaacatcg gcttcgcggg agaaagacga
      481 tttcgatggg tagaaactgt tagttattcg aagtcagaga cagcaggtaa ggctttttaa
      541 acgatccggt cggcctgccg agacttaggt tgtcataata ggagactttc ctgtgtgcgc
      601 tctggctctt tggatgatcc ggttgaggaa aaggtaatcc ggaaaatcag agtgaaaggg
      661 ggttaggtat tggatcgcaa gacgaaagag gccatggtgg ccgaacttca cgagaagctc
      721 aaggacgtca aactggccgt tctcgcagat tacagcggaa tgacggtggc caagatggag
      781 gcgttgagga gcggcttgcg taagaccggc gcggaaatgc gggtgatcaa gaatacactg
      841 ttgcggattg cctcgaaaga cacggacttt gctgctctcg acaggtatct gacaggcccg
      901 gttgctgtca tcctgaatta cggcgacatc gtcgagtcca cgaaggttct tgtagatttc
      961 gcgaagaaga acgcagagct ggaagtaaag gcgggcttca tgggagggga cgttcttagc
     1021 agggagcaga taaacaccct ggcagagctg cccagccgtg aggtcctgct cggcaaactc
     1081 ctctcggtca tggttgcagc ccagacgggc cttgttcgcg tgttgagcgg cgtgccgcga
     1141 agccttgtcc aggtgctcga tgcctatcgg atgaaaatgg tgtagaataa acatacagga
     1201 agggacgaaa aaatgacgga agtaaaggta acgaaggaag atgtcatcag ttttatcgaa
     1261 aacatgaccg tcctcgaact ctccgagctc gtgaaagagc tggagaagag gctcggcgtg
     1321 acggccgcgg ccccagtggc ggttgccgct gtggcggcgg gtggcgcggc gccggccccc
     1381 gcggaggagg agaagacgga gttcgacgcg atcctgacag ggttcggcga ccagaagata
     1441 caggttatca aggtcgtcag ggccatcacg ggtctcggtt tgaaggaagc taaggacctt
     1501 gttgaagggg tgccgaagcc gatcaaagaa ggcattccta aggccgaagc cgaggatata
     1561 aaaaagaaaa ttgaagaagt gggcggtaca gtcgagatca agtaggttga gccggtcggc
     1621 gggggccttt gccgagcaaa aggtggcctg ccgatttttg aggacgaccg gtgaaagact
     1681 atcggagtca ggagccagaa tctaaggaat ttgctttgtt ctgtctcctg acttcactac
     1741 ttttatcggc cggatagagg tggaaaagga aacaacagaa caggcaggtt gcatca
//
LOCUS       DAOTMX010000330         1737 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_53599_length_1737_cov_3.386445, whole genome shotgun sequence.
ACCESSION   DAOTMX010000330 DAOTMX010000000
VERSION     DAOTMX010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53599_length_1737_cov_3.386445"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="P5573_08390"
     CDS             <1..363
                     /locus_tag="P5573_08390"
                     /inference="COORDINATES: protein motif:HMM:NF012743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HRT28119.1"
                     /translation="PAIAESIFTFFHEPRNLAFLGKLEEAGIRPVAGKKTVSSLLKGK
                     TFVFTGGLKGFSREKAKEMVESLGGTAASSVSKKTHYVVAGEDPGSKYEKAKSLGVPI
                     LDEDGFLRLIRDMESRQG"
     gene            373..693
                     /locus_tag="P5573_08395"
     CDS             373..693
                     /locus_tag="P5573_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HRT28120.1"
                     /translation="MFSLSPLALCLMPDMKHVHVVVSGRVQGVFFRARTRDEAIRNNV
                     RGWVRNLPDGRVEAVFEGNSDDVDRLVDWCRVGPSQAVVEHVEAVEEPFTGAFQAFEI
                     RYRP"
     gene            818..1099
                     /locus_tag="P5573_08400"
     CDS             818..1099
                     /locus_tag="P5573_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT28121.1"
                     /translation="MLVLCLGLLLAAIPATAATTTDQIVTQIDDILKANPLSPTDKVQ
                     MIPVAQDDTITINVARFIEWAEVKPHLHKTHDEMVYVLKGTGQMFVNEK"
     gene            complement(1264..1392)
                     /locus_tag="P5573_08405"
     CDS             complement(1264..1392)
                     /locus_tag="P5573_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT28122.1"
                     /translation="MNGMSGLLEGDGDLDRAFGVEANVVGLDDHREREILNGIFST"
BASE COUNT          414 a          496 c          464 g          363 t
ORIGIN      
        1 cccgccatcg ccgagagcat cttcacgttc ttccacgaac cccgcaacct cgcgttcctg
       61 gggaaactcg aagaagccgg tatccggccg gttgcgggca aaaagaccgt ctcgtcgctc
      121 ctcaagggga agaccttcgt cttcaccgga gggctcaagg ggttctcgcg ggaaaaggcg
      181 aaggagatgg ttgaatccct cggcggcact gccgcatcct ccgtgtcgaa gaagacgcac
      241 tacgtcgtcg ccggcgagga ccccggctcg aagtacgaga aggcgaaatc tctcggcgtg
      301 cccatcctcg acgaggacgg cttcctgaga ctgatccgcg acatggaaag ccggcagggc
      361 tgattcagct ttttgttttc tctttcgcct ttagcccttt gtcttatgcc tgacatgaaa
      421 cacgtccatg tcgtcgtctc gggaagggtc cagggcgtat tcttccgggc caggacccgt
      481 gatgaggcca tccgaaacaa cgtgcgggga tgggtgcgca accttcccga cggccgcgtc
      541 gaggcggtct tcgaggggaa ctcggacgac gtcgatcgcc tcgtcgactg gtgccgcgtc
      601 ggccccagcc aggccgtcgt cgaacacgtg gaggccgttg aggaaccctt cacgggcgca
      661 ttccaggcgt tcgagatccg gtataggccc tgagaaggga atgtcggttc acatgctatt
      721 tgactcctct ttgatatggc caagaaacag cacaaacaat cgaatgaaat gcaaaaggag
      781 gacatgcgat caaaaaaagc aaatgacata gagcggaatg ttggttcttt gcctggggct
      841 gttattggct gctattcctg caacggcagc aactaccacg gatcaaatcg ttacccagat
      901 cgatgatatc ctcaaagcaa atcccctgtc gccaacggat aaggttcaaa tgatccctgt
      961 cgctcaggac gatacgatca cgatcaacgt ggcaaggttc atagaatggg cggaagtgaa
     1021 gcctcatttg cataaaactc acgatgaaat ggtctatgtg cttaagggaa cggggcagat
     1081 gtttgttaat gaaaaatagg ttgatgttaa accaagcacc ttccatttca atccaatgaa
     1141 taaggtccac gcaacgaaaa atacggggaa agaggactta gtcatttttt caatatttac
     1201 tcctcccatg aaagaaatgg atcgtcattt tgtagacgcg aaataggatc gacacctgat
     1261 ctttcacgtt gagaagatcc cgttcagtat ctctcgttca cgatggtcat caaggcccac
     1321 tacgtttgcc tcgaccccga aggcccggtc gagatctccg tcaccttcaa gtaagcccga
     1381 cattccgttc atcgctcagc ggccatggcc ccaccggcga catggccgct ttcgcatctg
     1441 gctgtcggat gaccggtttt cttgacaggg cgggaaatct cctttacagt gctgcatctg
     1501 caggcacctc cggccggaaa accggggacc tcccgtgcac ggaccgagtg tgtgcctgct
     1561 ggggatacga attcacaagc ggcaacccga tcaacccatg aaaccaaacg acaagacacc
     1621 ccccggcacg gaacccctgc gaccatctga ttcggaatcc cacttccccg cccgctcccg
     1681 aggcagcgac cacatcttcc ggacgctcac ggagaaatcc ttcgtgggga tgtacgt
//
LOCUS       DAOTMX010000331         1723 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_54215_length_1723_cov_2.697842, whole genome shotgun sequence.
ACCESSION   DAOTMX010000331 DAOTMX010000000
VERSION     DAOTMX010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54215_length_1723_cov_2.697842"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="P5573_08410"
     CDS             <1..460
                     /locus_tag="P5573_08410"
                     /inference="COORDINATES: protein motif:HMM:NF021625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine-phosphate synthase family protein"
                     /protein_id="HRT28123.1"
                     /translation="GYALPRAFGKEDVAAFPGRIIRVGTGVRPVEAPAFGASSHIAKI
                     ILTAMKYDGTYRSAMNIRFSTEAIDACRLLGFHVESFDRSCEPPEVKLREGSSLEWGT
                     DTVLARGAGMPDIIFDRGDVGKEPMIRVIGRSPDDVVEKVIRISRKLKER"
     gene            482..1264
                     /locus_tag="P5573_08415"
     CDS             482..1264
                     /locus_tag="P5573_08415"
                     /EC_number="2.4.2.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR00292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide-dependent adenosine diphosphate thiazole
                     synthase"
                     /protein_id="HRT28124.1"
                     /translation="MELNEVIITKAIIERFSQKLLEYTEVDVAVVGGGPAGLVASYFL
                     AGAGKKVAIFERKLSIGGGMWGGGMMFNEIVVQEEGKKVLDIFDVRTREYVPGYYTAD
                     AIEAVTTICSKAVKAGARIFNCITVEDVVIRDGRITGLVITWSPVEMTGLHVDPLAIS
                     TRCVVDSTGHDTEVLEVIERKADVRLNTETGKRLGERSLWADKAERLTIENTREICPG
                     VYVAGMAANAAFGGPRMGPIFGGMLLSGKKVAELILKTESFA"
     gene            1305..>1723
                     /locus_tag="P5573_08420"
     CDS             1305..>1723
                     /locus_tag="P5573_08420"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HRT28125.1"
                     /translation="MTQLEFARKGVVSEEMRICADSEGVNPEFIRNGVEDGSIVVVRN
                     AVRKNISPLAIGKGLRTKVNANIGTSRDHADLAQELEKVRVSVSAGADTIMDLSTGGD
                     IRGIRRAIIEAAPLAVGTVPIYQAAAKMLNEGRAISE"
BASE COUNT          399 a          474 c          545 g          305 t
ORIGIN      
        1 cggctatgcc ctgccccggg ctttcggaaa agaggatgtg gcggcattcc cggggaggat
       61 cataagggtc ggcaccggcg tgcggccggt ggaggccccg gccttcggcg cctcgagcca
      121 tatcgcaaag ataatcctga cggccatgaa gtatgacggg acgtaccgct ccgccatgaa
      181 catccgtttt tccacagagg ccattgacgc ctgcaggttg ctcggcttcc atgtagaaag
      241 tttcgaccgg tcctgtgaac caccggaagt caagctccgg gaaggttcgt ctctcgaatg
      301 gggaacagac actgttcttg cgaggggcgc cgggatgccc gacatcatct tcgaccgagg
      361 agacgtggga aaggagccga tgatccgggt cattggcagg tcaccggacg acgtagtgga
      421 aaaagtaatc cgtatatcaa gaaaattgaa ggagcgctga ttagtaagga ggagaacgcg
      481 catggagttg aatgaagtca ttatcacgaa ggccatcatc gaacgctttt cgcagaaact
      541 cctggaatac acggaggtcg atgtggccgt cgtcggcggc ggcccagccg gactcgtcgc
      601 ttcctacttc cttgccgggg cgggaaagaa ggtcgcgatc ttcgagcgga agctttccat
      661 cggcgggggt atgtggggcg gggggatgat gttcaatgag atcgtggtgc aggaggaagg
      721 gaagaaggtc ctggacatct tcgacgtaag gacccgtgag tacgtaccgg gctactacac
      781 ggcggacgcg atcgaggcgg tcaccaccat ctgctcgaag gccgtcaagg cgggcgcgcg
      841 catcttcaac tgcataaccg tggaggacgt tgtgattcgc gacggacgca tcaccggcct
      901 cgtcatcacc tggtcacccg tcgagatgac ggggctgcac gtagaccccc ttgccatctc
      961 cacccggtgc gtcgtcgact ccaccggcca cgacacggaa gtcttggagg tcatcgagcg
     1021 aaaggccgac gtgagactca acactgaaac gggaaagcgg ctgggagagc gctccctgtg
     1081 ggccgacaag gccgagcgac taacaatcga aaacacgcgg gagatatgcc ccggagtata
     1141 cgtagcgggc atggcggcaa acgccgcctt cggcggcccg aggatggggc ccatcttcgg
     1201 cggtatgctg ctatccggca aaaaggtcgc cgagcttatc ctcaagacgg agagctttgc
     1261 gtaatacagt aaatctatca tcacaaaaaa ccggttgcca taagatgaca cagctcgaat
     1321 ttgcacgaaa aggcgtcgtt agcgaagaaa tgagaatctg cgccgacagt gaaggggtga
     1381 atcccgagtt cattcgcaac ggtgtcgaag acggcagtat cgtcgtcgtc cgtaacgccg
     1441 tccgtaagaa catctcccct ctcgctatcg gcaagggact gcggacgaag gtaaacgcca
     1501 atatcggcac ctcgagagac cacgccgacc tggcgcagga gctggaaaag gtccgcgtct
     1561 ccgtttccgc cggggccgac actatcatgg acctatcgac gggaggcgac atccgcggta
     1621 tccgcagggc gattatcgag gccgcgcccc tcgccgtcgg gacagttccc atctatcagg
     1681 cagcggcgaa gatgctcaac gaaggaaggg ctatatccga gat
//
LOCUS       DAOTMX010000332         1703 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_55147_length_1703_cov_4.160801, whole genome shotgun sequence.
ACCESSION   DAOTMX010000332 DAOTMX010000000
VERSION     DAOTMX010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55147_length_1703_cov_4.160801"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..93)
                     /locus_tag="P5573_08425"
                     /pseudo
     CDS             complement(1..93)
                     /locus_tag="P5573_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895030.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
     gene            complement(154..1146)
                     /gene="trpS"
                     /locus_tag="P5573_08430"
     CDS             complement(154..1146)
                     /gene="trpS"
                     /locus_tag="P5573_08430"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HRT28126.1"
                     /translation="MSRKRILSGMRPTGRLHLGNLHGALKNWVELQDSGNYECFYFVA
                     DWHALTSDYASTEQIRSYTLDMIVDWLSVGLDPEKSTIFIQSRVKEHAELFLLLSMIT
                     PISWLERNPAYKEMRAELTDRDLSTFGFLGYPVLQAADIIMYKAYGVPVGVDQLPHVE
                     LTREIARRFNFLYGGEVFPIPEALLTTVPKVLGIDGRKMSKSYENAIYLRDRGADLKK
                     KVESMFTDPQRMTKKDPGRPELCNVFTFHGIYSPEQDVKEIETECRRAGIGCVECKRR
                     LADRIIDGLRPIHERQDHYLGHMDEVRGIIEEGSSRAAAVARATMEEAREAVKI"
     gene            complement(1326..1598)
                     /locus_tag="P5573_08435"
     CDS             complement(1326..1598)
                     /locus_tag="P5573_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRT28127.1"
                     /translation="MTKADLIAAMADDAGITKAAAAKALESYIANVAKELKKNGKLGI
                     VGFGTFTVTKRKARSGRNPQTGEKIKIAAKKVIKFKPGKALADKVK"
BASE COUNT          334 a          513 c          428 g          428 t
ORIGIN      
        1 agctatgggg atgttgtaga tgtcgacctc gtttttcttg atgagataga ggagcaggtc
       61 cagcggacct tcaaagatgt cgagcttgac ctcgtacgtc atccccgcca acccctgaga
      121 cctgtggaat ccccggcagg aatcccgccg gcttcaaatc ttcacggctt ctctggcctc
      181 ttccatcgtg gcgcgtgcaa cggccgccgc ccggctgctg ccctcttcga tgatgccgcg
      241 cacctcgtcc atgtggccga ggtaatgatc ctggcgctcg tgaatcggcc tgagaccgtc
      301 gatgatccgg tcggcaagac gcctcttgca ttcgacgcag ccgatgcccg ccctccggca
      361 ctcggtttcg atttctttca cgtcctgctc cggggaatag attccatgga aggtgaagac
      421 attgcagagc tccggcctgc cggggtcctt cttggtcatg cgctgggggt cggtgaacat
      481 cgattccacc ttcttcttca ggtctgcgcc gcggtcccgc agatagatgg cgttttcata
      541 agacttgctc atctttcggc cgtcgatgcc gagtactttg gggacggtcg tcagaagggc
      601 ctcggggata gggaaaactt cacctccgta aaggaagttg aaacgccggg cgatctccct
      661 cgtcagctcc acatggggaa gctggtccac acccacgggg acgccatagg ccttgtacat
      721 gatgatgtcg gccgcctgca ggaccggata ccccaggaaa ccaaaggtcg agaggtcccg
      781 gtcggtgagt tcggccctca tctccttata cgccgggttc cgctccaacc atgaaatggg
      841 ggtgatcatg gagagaagga gaaagagctc ggcatgttcc ttgacccgcg attggataaa
      901 gatcgtgctc ttttcagggt cgaggccgac actcaaccaa tcgactatca tgtccagggt
      961 atagcttcgg atctgttccg tactggcata atcgctcgtc aacgcatgcc agtccgctac
     1021 aaaatagaaa cactcgtaat tgccgctatc ctgaagttca acccagtttt tcaaggcccc
     1081 atgcagattt cccaggtgta acctgcctgt aggcctcatt ccgctcaata ttcttttcct
     1141 ggacactgac ctcttctccc ctgttccgct ctctgctcta tgccttgaag cgcgttatca
     1201 ttaacagaag ggaccggggc ttgtcaatcg agctctccac cggcgagcta tccgcaacgg
     1261 aacataaaaa ccaagacccc cgctcgggtt ccaaacgggg gtcttagttt acgcgcggct
     1321 gtgtgctatt ttactttgtc ggcaagagct ttgccgggct tgaacttgat gaccttcttc
     1381 gctgcgatct tgatcttttc gccggtctgg ggattcctgc cgctccttgc cttcctcttc
     1441 gtaaccgtaa aagtgccgaa tcctacgatt cctagcttac cgttcttctt taactccttg
     1501 gccacgttcg cgatgtatga ttccagtgcc ttagcggcgg cggccttcgt tattcctgca
     1561 tcgtccgcca tcgctgctat caaatccgcc tttgtcatac cttcccccct ttctcatctc
     1621 agtcaaatta tactatcgaa tggcgcccaa actgaacaaa cgccccgatt atccggaaag
     1681 aaaactgttc aactcctgcc ttt
//
LOCUS       DAOTMX010000333         1670 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_56775_length_1670_cov_2.673684, whole genome shotgun sequence.
ACCESSION   DAOTMX010000333 DAOTMX010000000
VERSION     DAOTMX010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56775_length_1670_cov_2.673684"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /gene="purN"
                     /locus_tag="P5573_08440"
     CDS             <1..476
                     /gene="purN"
                     /locus_tag="P5573_08440"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HRT28128.1"
                     /translation="FDRKMVEILRTYSVELVVMAGFMRVLTDLFLQAFPMRVMNIHPA
                     LLPSFPGLHGQQKAFDYGVKFSGCTVHFADSGVDTGPIIIQAVVPVHDDDTADSLAER
                     ILKEEHRIYPQAIQLFSEGKLTVSGRKVRVKNHTKAPDKPIHNPPLLIRELTRGS"
     gene            473..>1670
                     /locus_tag="P5573_08445"
     CDS             473..>1670
                     /locus_tag="P5573_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT28129.1"
                     /translation="MTKSYDVIVIGNDLGSLVATELLSRSGKKTLLIKEGKVPDVYSE
                     SGYTFNLDPSIPAGLGTLDVAVHPYLENDLYQMNRAALKMLNPGLQIITPSHRVDVFR
                     ERDDLIRDMEREFPGSSPQMNELYDAAERTALVFSRFTLENPSLCIPWRSLKTMTHFA
                     RALLHLHREHLKFVRRLEGLDDNLVCLLDTQASGLSGMVHSCGDGSMTPATPYFLSLP
                     LKGLFYCAGGKHLIAGSLEERFLRQGGECISGCEVTGIDVEGGHRRIAWAAEGIIGES
                     LGESLIVSYKWKKLFDCLLNNRKGRRFSQRLNRRRAKAFYPFTVHMGVFDKGIPERLA
                     EYAVILGKETGEFRIQEVIYLELSQRGDTGRAPEGRRALSATRYIDEPPERLGDEDLR
                     RVAAGLL"
BASE COUNT          394 a          466 c          451 g          359 t
ORIGIN      
        1 atttcgacag aaagatggtc gagattttgc gaacctacag cgtcgaactg gtcgtgatgg
       61 caggtttcat gcgggttttg acggaccttt tcctccaggc ctttccgatg agggtgatga
      121 acatccatcc cgcccttctg ccttctttcc ccggcctcca cggacagcag aaggcctttg
      181 attacggcgt gaaattctcc ggttgcaccg tacatttcgc cgattccggc gtcgacacag
      241 gaccgataat catccaggcc gtcgtccccg tccatgatga cgataccgca gatagtctag
      301 cggaaaggat attgaaagag gagcacagga tatatccgca ggcaatccag ctcttttccg
      361 aggggaagct gacggtctcg ggccggaagg taagagtaaa aaaccacacg aaggccccgg
      421 ataaacccat ccacaaccct ccattactca tcagggagct tacccgcggg tcatgacgaa
      481 aagctacgat gtgatcgtta tcggaaacga cctcggctcc ctggtcgcaa ccgagctttt
      541 gtcccgttcc ggcaagaaaa cgctcctcat caaggaaggc aaggtgccgg atgtctattc
      601 cgaatcgggc tatactttca acctggaccc ttccataccg gccggcctcg gaacccttga
      661 cgtcgccgtc catccttatc ttgaaaatga cctatatcaa atgaacaggg cggccttgaa
      721 gatgctgaat cccggactcc agatcatcac accgtcccat cgggtggatg ttttccgcga
      781 aagagatgat ttgatacgcg acatggagag agaattcccg ggtagctcgc cccaaatgaa
      841 tgaattgtac gacgccgcgg aaaggacggc cctcgtcttt tcccgattca ccctcgaaaa
      901 cccctctctg tgtatcccgt ggcgctctct aaagacaatg acacactttg caagggcgct
      961 tctacatctt cacagggagc atttgaagtt cgtccgtagg ctcgaaggtc tcgacgacaa
     1021 cctcgtctgt ctcctcgaca cccaggcatc cggcctctcc ggcatggtcc atagctgcgg
     1081 cgacggatcg atgacccccg caacccctta cttcctgagc cttccattga aaggcctgtt
     1141 ctattgtgcc ggaggaaagc accttattgc cggctccctt gaggagcggt ttttgcggca
     1201 aggcggtgaa tgcatcagcg gctgtgaagt caccgggata gacgtcgagg gcggacatcg
     1261 ccgcatcgct tgggccgcag agggcatcat cggcgaaagc ctgggggaaa gccttatcgt
     1321 aagttacaaa tggaagaaac ttttcgattg tcttctcaac aaccgaaaag gacggaggtt
     1381 ttcgcaacgc ctgaacaggc gtagggcgaa ggccttctat ccttttaccg tccacatggg
     1441 cgtcttcgac aaaggaatcc ctgaaagatt agccgaatat gccgtaatcc tcggcaagga
     1501 gacgggcgaa ttcaggattc aggaagttat ctacctggaa ctgagtcaac gcggagacac
     1561 ggggcgggct ccggaaggca gaagggcgtt gagtgcgacg cgttacatag acgaaccccc
     1621 tgaacgactg ggagacgagg acttgcgcag ggttgctgcg ggtcttttgg
//
LOCUS       DAOTMX010000334         1666 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_56946_length_1666_cov_4.334575, whole genome shotgun sequence.
ACCESSION   DAOTMX010000334 DAOTMX010000000
VERSION     DAOTMX010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56946_length_1666_cov_4.334575"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..903)
                     /locus_tag="P5573_08450"
     CDS             complement(112..903)
                     /locus_tag="P5573_08450"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRT28130.1"
                     /translation="MNFEHIVYTKENGVATIKFNRPKSFNSLDLDTSCEFVAAVEKCT
                     EDVDVRSVIITGEGKAFCSGGDIAYFKKFLETAPSEPFRDVMKYLNLAIIGIRRMPKP
                     VIAAVNGAAGGAGMSVAAACDLRICAASAKFRQAYTGIGMVGDEGWTLFVPLLIGFGR
                     TMELLLLDPIFDAKQALEWGLVTQVVEDEKLKDTTLEIAAKLAQGPTKAYAIAKENLN
                     RSMMANLESQLELERIGMMAASRTEDYVEGVRAFLEKRKPVFKGR"
     gene            complement(1043..>1666)
                     /locus_tag="P5573_08455"
     CDS             complement(1043..>1666)
                     /locus_tag="P5573_08455"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRT28131.1"
                     /translation="PKGKGISTFLIENGTKGLTIGREEHKMGQHASATNELIFEDCRV
                     PKSALMGKLNDGFRTAVGELAGGRIGIGSLALGCGLAAMDYATAYAKERRQFDQPITG
                     FQAIQWMIAESYTELEAARLLLMNAAFKKEQGRFFARDASMAKYYATEAAERACYNAI
                     QMLGGYGYTAEFPVERYARDVRVTSIYEGTNQIQRVIIARDILSSLK"
BASE COUNT          347 a          464 c          424 g          431 t
ORIGIN      
        1 cgaattccct gacttcctgc tttatcgcct tctcttcctc ggtcaataag acgtcgagcg
       61 gcatcgacaa tctcccttca gatagtctat ctttatgaag atgcttccga atcagcggcc
      121 tttgaaaaca ggcttgcgct tttccaagaa agccctgact ccttcgacgt agtcctctgt
      181 cctggaagca gccatcattc cgatcctctc gagttcaagc tgcgattcca gattagccat
      241 catggagcgg ttcaagtttt cctttgctat agcgtatgcc ttagtcgggc cctgtgcgag
      301 tttcgctgcg atttctaagg tcgtgtcctt taatttctca tcttccacga cctgcgtgac
      361 taggccccac tcaagggcct gttttgcgtc gaatatgggg tcaagaagga gcagttccat
      421 ggtcctccca aacccgatca gaaggggtac aaatagcgtc cagccctcgt cacctaccat
      481 gccgatgccg gtataggcct gtctgaattt tgcggaggcc gcgcatatcc tgaggtcaca
      541 ggctgcagcg acgctcatcc cggcaccgcc ggcggcaccg ttgacggcgg cgatgacggg
      601 cttgggcatc cggcggatac cgatgatcgc gagattcagg tatttcatca cgtcgcgaaa
      661 cggttcagat ggtgcagtct cgagaaattt cttgaaatag gcaatatccc cgcctgaaca
      721 aaacgccttg ccttcgccgg tgatgataac cgagcggaca tcgacgtctt cagtgcattt
      781 ttcgacagcg gcgacaaatt cgcagctggt atcaaggtcg agtgaattaa aagattttgg
      841 cctgttaaat ttgattgtcg cgacgccgtt ttcttttgtg tacacgatat gctcgaagtt
      901 catctaatga ccctccttgc gctgataaat aacaatgata gggtagctgg gatgtttccg
      961 gtccgggttt aatctcccgg accggactgt taacgttatg aatctaaaga ataacaaaca
     1021 gttggcagga tacgctgatt ttttacttga gactgctcag gatgtctctg gcgataatca
     1081 ccctctgaat ctgatttgtc ccctcgtaga tcgaagtaac gcgaacatcc cttgcatagc
     1141 gttcgaccgg aaactcggcg gtgtaaccgt aaccgcccaa catctgtatc gcgttgtagc
     1201 aggccctttc cgccgcctct gtggcatagt atttcgccat cgaggcgtca cgggcgaaga
     1261 agcggccctg ctctttcttg aaggcagcgt tcatcaagag aagtctggcc gcctccagtt
     1321 ccgtgtatga ctcggcgatc atccactgga tggcttgaaa gccggtgatg ggctggtcga
     1381 attgccggcg ctcctttgca taggccgtcg cgtaatccat ggctgcaagg ccacagccga
     1441 gggcgagcga cccgatgcct atccgtccac cggcgagttc tcccaccgcc gtccgaaaac
     1501 cgtcgttgag ttttcccatg agagcactct taggaactcg gcagtcttca aagatgagct
     1561 cgttcgttgc cgacgcgtgt tgacccatct tgtgctcctc tcggccgatg gtcaatccct
     1621 tcgtcccgtt ttcgatcagg aacgtgctga tgcctttgcc cttggg
//
LOCUS       DAOTMX010000335         1663 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_57095_length_1663_cov_3.995025, whole genome shotgun sequence.
ACCESSION   DAOTMX010000335 DAOTMX010000000
VERSION     DAOTMX010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57095_length_1663_cov_3.995025"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="P5573_08460"
     CDS             complement(<1..699)
                     /locus_tag="P5573_08460"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HRT28132.1"
                     /translation="MIGDNIKPLPTTRWEHTDIDDKIPELLSAELGLHPIVGRVLACR
                     SFQDPESASKHLFPRLIDLHNPFLMKDMKAGVDRVIRSLHSKERIVIFGDYDADGITS
                     LVILYKFLQNLGAPVSYYVPDRIKEGYGLTKGVVEKIRAGGAGLLITVDCGISNHEEI
                     ALASSIGLETVVLDHHEVAGSLPKAAAVINTNRGDCPFPFKQLAGVGIVFNFLIALRA
                     KLRDAGFWRNKTYPN"
     gene            complement(810..1007)
                     /gene="rpmB"
                     /locus_tag="P5573_08465"
     CDS             complement(810..1007)
                     /gene="rpmB"
                     /locus_tag="P5573_08465"
                     /inference="COORDINATES: protein motif:HMM:NF013026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HRT28133.1"
                     /translation="MSRVCEICGKGPVVGHNVSHANNKTKKVWYPNLHKTKVIDPKTG
                     TRKSMKICANCLRSGSHKKAV"
     gene            complement(1101..>1663)
                     /locus_tag="P5573_08470"
     CDS             complement(1101..>1663)
                     /locus_tag="P5573_08470"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HRT28134.1"
                     /translation="HIAASAALAQGDANFVLIPEIPFDLEGGKGFLPTLERRLRERHH
                     AVILVAEGAGQDLLHRNGMPVRTDPSGNVRLADIGLFLKERIEEYFKQRGMEVNLKYI
                     DPGYIIRSVIANASDSLYCGVLAKYAVHAAMAGKTGMVVGLVNNTYVHLPLRAVASRR
                     KMVDPNGNIWMNVLESTGQPASLKND"
BASE COUNT          339 a          435 c          459 g          430 t
ORIGIN      
        1 gtttgggtac gtcttgtttc tccaaaaccc ggcgtctctt agtttggccc gcagggcgat
       61 gagaaaattg aacacgatgc cgacgccggc taactgcttg aaagggaaag gacagtcgcc
      121 tctgtttgtg ttgatcaccg ccgctgcctt cgggagtgat ccggctactt catgatgatc
      181 caagacgact gtttccaggc cgatagagga agcgagggct atttcttcat gatttgatat
      241 accgcaatcc acagtgatga gcagcccagc cccaccggcc cgaatctttt caacgactcc
      301 ttttgtgagg ccgtagcctt ctttaatcct gtcgggaaca tagtagctta ccggtgctcc
      361 gaggttttgc aagaacttgt atagaatgac gagggaagtg ataccgtcgg catcgtagtc
      421 gccgaagata acgatccttt cctttgaatg aagggagcgg ataacgcggt ctacccccgc
      481 cttcatatcc ttcatgagga aagggttgtg gaggtctatc aaccggggga acagatgctt
      541 cgaggcgctt tccgggtcct ggaaagaccg gcaggcgagc acgcggccga caatgggatg
      601 aagtcccagc tccgccgaca ggagctccgg aatcttgtcg tcgatgtccg tgtgctccca
      661 tcgagttgtt ggcaggggtt ttatgttgtc accaatcacc tgaatcgctt tcgaaaaggc
      721 cgcccttatg ggtaggatgt agcgagaacc gatgacggtt acggccgctc gtccacagaa
      781 cggccctctg cagtgtcatg ctgacgctgc tacaccgctt tcttgtgcga accggagcga
      841 aggcagttgg cgcagatttt catgctcttc cgtgtgccgg ttttcgggtc gattaccttc
      901 gtcttgtgca ggttgggata ccagaccttt ttcgttttgt tgtttgcatg gctaacattg
      961 tgacctacta cagggccttt gccgcatatc tcgcagactc gtgacaatgt ctcttacctc
     1021 cattacagaa aagttgctta tgtttgcacc ggtataccta accaatatga atttggattt
     1081 caagtacttt ttgttgcgcc tcagtcgttt ttcaaggatg ccggttggcc tgtcgattcg
     1141 aggacgttca tccagatgtt gccgttgggg tcgaccatct tgcgtcttga agccacggcc
     1201 cttaaaggca gatggacgta tgtgttatta acgagcccca cgaccatacc cgtttttccg
     1261 gccatcgctg catggacggc gtacttggcg agtactccgc agtagaggct gtcgctggca
     1321 ttggcgatga cactccgaat gatgtacccc gggtcgatgt acttaaggtt gacctccatg
     1381 ccccgttgct tgaagtattc ctcgatcctt tctttgagga ataggcctat atctgcgagc
     1441 cgcacgttgc cggaagggtc ggttctgacg ggcatgccat tgcggtgcag caaatcctgg
     1501 ccggcccctt cagcaacgag gataaccgca tggtgtcttt cacgtagcct tcgctccaag
     1561 gtaggcagga acccctttcc cccctcgaga tcgaagggga tctcgggaat gaggacaaaa
     1621 tttgcgtctc cttgggctaa ggcggcgctg gccgcgatgt gac
//
LOCUS       DAOTMX010000336         1662 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_57142_length_1662_cov_3.367766, whole genome shotgun sequence.
ACCESSION   DAOTMX010000336 DAOTMX010000000
VERSION     DAOTMX010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57142_length_1662_cov_3.367766"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..695)
                     /locus_tag="P5573_08475"
     CDS             complement(54..695)
                     /locus_tag="P5573_08475"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRT28135.1"
                     /translation="MREEQVYFESDGLTIEGLYAPRPGQRGVVVTHPHPLMGGSMRNN
                     VVMALVSVFQRYGFSTLRFNFRGVGRSGGVFDDGRGEVGDLRAAVSFLSDEGVTEIAL
                     AGYSFGAVVAASYLTEGSFIDRVVLVSPPLAEAGLEIPMLRGKKGLLVCGDDDYFCPV
                     DALKKVALQLSWGAAVIAGADHFYFGREKDLMDAVENWLAADVGPKSGEETRE"
     gene            complement(692..>1662)
                     /locus_tag="P5573_08480"
     CDS             complement(692..>1662)
                     /locus_tag="P5573_08480"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRT28136.1"
                     /translation="SGVLSRQVRLVRAVDGVNLRVFEGETLGLVGESGSGKSTLGRVV
                     IRLDAPTEGRVLFDGRDVHRLQKEELRSFRRDVQIVFQDPYASLNPRKSAESIIGEPL
                     TVQQICDRRERRERVVRLMEVVGLTPDQMRRYPHEFSGGQRQRIGIARAIATNPKLII
                     ADEPVSALDVSVQAQILNLLKDLQRDYNLTYIFISHDLRVVENMSDRIAVMYLGKVVE
                     VAQSSALCGEPLHPYTRALFSALPVMKPGERKERIILEGDVPSPSNPPAGCPFHPRCR
                     ERMKVCETQAPALTRTVEGRFVACHLIGDEAAGKGLGRKEERGALI"
BASE COUNT          339 a          548 c          421 g          354 t
ORIGIN      
        1 accctcggca tcccccgacg ccccggcacc cgttgatggc aatcgataag tcttcattct
       61 ctcgtctctt caccagactt cgggccgaca tcggcggcga gccaattttc tacggcatcc
      121 atcaggtctt tttccctgcc gaagtaaaag tggtctgccc ctgcgatgac cgccgcgccc
      181 cacgataatt gtagggcaac cttttttagg gcatccaccg ggcaaaaata atcatcatca
      241 ccgcagacga ggagtccctt tttccccctg agcatgggaa tctccaggcc ggcttcagcc
      301 aggggcggcg acacgagaac aactcggtct ataaaagaac cttcggtcag ataagaggcc
      361 gccacgacag ccccgaagga atagccggcg agggcgattt ccgttactcc ctcgtccgac
      421 aaaaacgaca cggccgctct gaggtctccg acttcccccc ggccgtcgtc gaaaacacct
      481 ccactcctgc cgacacccct gaagttgaaa cggagagtgc tgaatccgta gcgttggaaa
      541 accgagacca gtgccattac cacgttgttc cgcatacttc cacccatcaa cggatgggga
      601 tgggtaacta cgacgcccct ttgacccggc cgcggtgcat acagtccttc gatcgtcaat
      661 ccgtccgact caaagtaaac ctgttcctcc ctcatatcaa ggcacctctt tcctccttcc
      721 ttcccaggcc ctttcctgcc gcctcgtcgc cgataaggtg gcaggccaca aatcgtccct
      781 ccaccgtccg cgtgagtgcg ggcgcctgtg tttcacaaac cttcatgcgc tctctgcatc
      841 ggggatggaa gggacatcct gccgggggat tagagggact ggggacgtct ccttcgagaa
      901 tgatcctttc tttcctttcc cccggcttca tcaccggtaa agccgaaaaa agagcccggg
      961 tgtaagggtg aagaggttcc ccgcagagcg cactgctctg tgccacttcc acgaccttcc
     1021 cgaggtacat gacggctatc cggtcgctca tgttttccac caccctcaga tcatgggaaa
     1081 tgaaaatata cgtaaggttg taatcccttt ggaggtcttt caagagattg agaatctggg
     1141 cctggacgga aacatcgagc gcagagaccg gctcgtccgc tatgatcaac ttgggattcg
     1201 tggcgatcgc ccgggcaatg ccgatccgtt gcctctggcc gccgctgaac tcatggggat
     1261 accgcctcat ctgatcaggg gtcaggccga ctacctccat caatcggacg acccgctccc
     1321 ttctttccct gcggtcacaa atctgctgga ccgtcaacgg ttcaccgatt atgctttccg
     1381 cgctctttcg tgggtttagt gacgcatagg ggtcctggaa gacgatctgc acgtcccggc
     1441 ggaaggaacg cagctcttcc ttctgtaaac gatggacatc ccgaccgtcg aagaggaccc
     1501 ttccctccgt cggcgcgtcc agcctgatga cgactcgccc cagcgtcgac tttccggagc
     1561 cgctttctcc gaccagtccg agcgtttcgc cctcgaagac ccgcaggttc acgccgtcga
     1621 ccgcccggac gagacggacc tgccttgaca ggactccgct tc
//
LOCUS       DAOTMX010000337         1651 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_57708_length_1651_cov_4.840226, whole genome shotgun sequence.
ACCESSION   DAOTMX010000337 DAOTMX010000000
VERSION     DAOTMX010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57708_length_1651_cov_4.840226"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..858)
                     /locus_tag="P5573_08485"
     CDS             complement(112..858)
                     /locus_tag="P5573_08485"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRT28137.1"
                     /translation="MTVIDSHIHCGVQNTDLPFETVRRFIESGDIQGACLFAPVEDIY
                     DRYDYHFKDTPRWAACRQRANEYLLDIQRSRQDIFAYFFVWNDFRKEELKKGYRGVKW
                     HRHTCEPEYDYESPLCEEFLQEVYRLRLPIVLEESFENTRYFVRRVAGRTPVIIPHMG
                     GLNGGFRALFDSGIWDDEAVYADTALASGREIKTFFNRYGSTRLIFGSDFPFGIPYHE
                     FQTVAGLGLDREDVENIAHRNILRLVKADA"
     gene            complement(855..1598)
                     /gene="kdsB"
                     /locus_tag="P5573_08490"
     CDS             complement(855..1598)
                     /gene="kdsB"
                     /locus_tag="P5573_08490"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HRT28138.1"
                     /translation="MSVVCIIPSRYESSRFPGKPLADLCGKPMVRHVYDRVLASPVVA
                     LAAVATDDERIYRAVVGFGGRAVMTAPRHRSGTDRVAEAASLLGLGESDVVVNVQGDQ
                     PLFEPVQVQEVAQPLLEDPAVDMATLVYKIERKEEITHPNAVKVVFDRNHFALYFSRA
                     TIPYVRDKGLQASYYKHHGIYAYRKGFLDSFSRLEQTELERLEALEQLRALEHGYRIK
                     VVETQYDSVEVDTPAELERVRQILMEGMQ"
BASE COUNT          321 a          493 c          493 g          344 t
ORIGIN      
        1 ccggagaaga gcgccgcctc cctgcgctct ttcaagggat gggagggggg ctgagagcct
       61 tagccctctc cggaaattaa gccgcagaac gagcgaagag gtggggccgg gtcacgcatc
      121 cgcctttacc agccggagga tgtttcggtg tgctatgttt tcgacgtctt cccggtcgag
      181 gccaaggccg gcaaccgtct gaaactcgtg gtaggggatc ccgaagggaa agtcgctgcc
      241 gaagataagc ctcgtacttc cgtaccggtt aaagaaagtc tttatctccc ggcctgaggc
      301 cagtgccgtg tcggcataga cggcctcatc atcccagatg cccgaatcga agagcgcccg
      361 gaagccgccg ttcaaaccgc ccatgtgggg aatgatgacg ggcgtcctgc ccgccacccg
      421 ccggacaaag tagcgggtgt tttcgaagga ctcctccagg acgatgggaa gccgcagacg
      481 gtagacttcc tgcagaaact cctcgcacag ggggctttcg tagtcatact ccggctcgca
      541 ggtgtgccgg tgccacttga cccccctgta gccttttttc aactcctcct tccggaagtc
      601 gttccagacg aagaagtagg caaagatgtc ctgccgactc ctctggatgt cgagcaggta
      661 ctcgtttgcc ctctgccggc aggccgccca gcggggcgta tctttaaaat ggtagtcgta
      721 cctgtcgtag atgtcctcga ccggcgcaaa aaggcaggca ccttggatat cgccgctttc
      781 gatgaaccgc cggactgtct caaagggtag gtctgtgttc tggacgccac agtggatatg
      841 ggaatcgatg accgtcactg cataccttcc atgaggattt gtctcaccct ctccagttcc
      901 gcgggtgtgt cgacctcgac ggaatcgtat tgcgtttcca cgaccttgat ccggtagccg
      961 tgctccaggg cgcgaagctg ctccagcgcc tcaagccttt cgagctccgt ctgctcgagg
     1021 cgtgaaaagc tgtcgagaaa gcccttccgg taggcataga tgccatgatg cttgtagtac
     1081 gacgcctgaa gccccttgtc cctcacgtag gggatcgtcg cccgggaaaa gtagagggcg
     1141 aagtgattcc tgtcgaaaac gaccttgacg gcgttcgggt gcgttatctc ttctttcctt
     1201 tcgatcttgt agacgagcgt cgccatgtct actgcagggt cttccaagag aggctgcgcg
     1261 acttcctgca cctgcaccgg ctcaaaaagt ggctgatctc cctggacgtt cacgacgacg
     1321 tccgattcac ccaggccgag taaggacgcc gcttcggcga cccggtccgt ccccgaacgg
     1381 tgacgagggg ccgtcatcac ggccctaccg ccgaacccta caacggctcg gtagatacgc
     1441 tcatcgtccg tcgccacggc cgcgagggca acgaccggcg aggcgagcac gcggtcgtag
     1501 acgtgccgga ccatgggttt tccgcatagg tcggcgagcg gcttccccgg aaaccgggac
     1561 gattcgtagc gcgaagggat gatgcagacg actgacatgg ttggtgtctc tttggaaacc
     1621 cccttgttct ctacagatag gggtctttct g
//
LOCUS       DAOTMX010000338         1638 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_58386_length_1638_cov_3.392293, whole genome shotgun sequence.
ACCESSION   DAOTMX010000338 DAOTMX010000000
VERSION     DAOTMX010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58386_length_1638_cov_3.392293"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /gene="ribH"
                     /locus_tag="P5573_08495"
     CDS             complement(<1..258)
                     /gene="ribH"
                     /locus_tag="P5573_08495"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HRT28139.1"
                     /translation="MPKMIEGKLVAKGMRFGIVASRFNDFICSRLIEGAVDALVRAGA
                     EEKDIEVAKVPGAFEIPVAAKKLAKSAKYDAVICLGAVIRGA"
     gene            complement(293..1495)
                     /locus_tag="P5573_08500"
     CDS             complement(293..1495)
                     /locus_tag="P5573_08500"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HRT28140.1"
                     /translation="MAISTIKEAIDDIKKGKMVILVDDEDRENEGDLCMAAEHITPEA
                     INFMAKYGRGLICLSLSDDLADRLELPLMVRDNRSRFGTAFTVSIEARRGVTTGISAA
                     DRAVTIRTAVADGTMADDLVSPGHVFPIRARKGGVLVRTGQTEGSVDLARLAGLKPAG
                     VICEIMKDDGTMARMPDLEIFAKEHDLKIVTIADLIDFRLKTERLVRRVATASLPTRY
                     GGEFKIIVYENDVDDMKHVALVQGEIQPEDEVLVRVHSECLTGDTFGSERCDCGDQLH
                     TAMRMVGEAGKGVIVYMRQEGRGIGLANKIKAYELQEKGRDTVEANIELGFKEDLRDY
                     GIGAQILVDLGVRKMRLMTNNPKKIVGLQAYGLSVVERVPIEIEPTENNIHYLETKKK
                     KMGHILEL"
BASE COUNT          303 a          534 c          396 g          405 t
ORIGIN      
        1 cgcccctcgg atgaccgcac cgagacagat tacagcatcg tactttgcgc ttttggcgag
       61 cttctttgcc gccacgggta tttcgaaggc ccccggcacc ttggctactt cgatgtcctt
      121 ttcctccgcc cccgcccgga ccaaggcgtc gacggcgccc tcgatgagtc tactgcatat
      181 gaagtcgttg aatcggcttg ccacgatccc gaacctcatc cccttcgcta cgagtttccc
      241 ttctatcatc ttcggcataa tctttccctc cgcaaaaagc ttggcatctc gtttacaatt
      301 cgaggatgtg ccccattttt ttcttcttcg tctcgaggta atggatgttg ttctccgtcg
      361 gctcgatctc gatcgggacc cgttcgacca cggagagccc gtaggcctgg agtccgacga
      421 tcttcttggg gttgttggtc atcagcctca tcttccgcac accgaggtcg acgagaatct
      481 gggcgccgat accgtaatcc cggaggtctt ccttgaagcc gagttctatg ttcgcctcga
      541 cggtgtcccg gcctttttcc tgtaactcgt atgccttgat cttattggca aggccgatac
      601 ccctgccttc ctggcgcatg taaacgatga cgcccttccc cgcctcaccg accattctca
      661 tggccgtgtg gagctggtcg ccacagtcgc aacgctcgga gccaaaggta tctccggtca
      721 ggcactcgga atggacgcgg acgagcacct catcttcagg ctggatctct ccctgcacga
      781 gggcgacgtg tttcatgtcg tcgacgtcgt tttcatagac gattatcttg aactccccac
      841 cgtagcgggt gggaagactg gccgtcgcca cccgcctgac cagacgctcc gtcttcagcc
      901 ggaagtcgat aaggtcggcg atcgtgacta tcttcaagtc atgttctttt gcgaaaatct
      961 ccaagtccgg catccgggcc atcgtcccgt cgtctttcat gatctcgcag atcacacccg
     1021 ccggtttcag gccggcgagc cgcgcgaggt ccacggaccc ctccgtctgt ccggttctta
     1081 caaggacacc gccctttctc gcccggatgg gaaaaacatg ccccggactg accaggtcgt
     1141 ccgccatagt gccgtccgcg acggccgtcc tgatcgtcac agcacggtcg gccgccgaga
     1201 tacccgtcgt cacgccacgg cgtgcctcga tggaaacggt gaaggccgta ccgaaccgcg
     1261 accgattgtc ccgcaccatc agtgggagct ccaatctgtc ggcaagatcg tcactcaggc
     1321 tcagacagat aagaccccgg ccatactttg ccataaagtt gatcgcctcc ggggtgatat
     1381 gttctgccgc catgcagagg tctccttcat tttcccggtc ttcatcatcg accaggatga
     1441 ccatcttacc cttctttata tcgtcgattg cctcttttat agtactgatt gccataaaaa
     1501 caccttataa ccgactactc atattatttg atgtacccat gctccgccag aaagttgagg
     1561 tcgatgggct tcctaacgcc accctcgact tcatccttcc gcatcagctt ctccacgtac
     1621 tttccgagta tatccgtc
//
LOCUS       DAOTMX010000339         1603 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_60374_length_1603_cov_4.266150, whole genome shotgun sequence.
ACCESSION   DAOTMX010000339 DAOTMX010000000
VERSION     DAOTMX010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60374_length_1603_cov_4.266150"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="P5573_08505"
     CDS             complement(<1..849)
                     /locus_tag="P5573_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016099277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRT28141.1"
                     /translation="MEFIKVRGASQHNLKHLDLDIPRNRLVVITGVSGSGKSSLAFDT
                     VFAEGQRRYVESLSTYARQFIGRMDKPEVESIEGLSPAIAIEQRQPSQNPRSTVGTVT
                     EIYDYLRLLYARVGIPHCYRCGREIKPQTVEMMMDYVLALPEGTKVSLLAPVVTGEKG
                     EFQKELRKLRKDGFVRVRVDGTVHDLADEIPLDRKKRHSIDVIVDRLIRREGIEKRLR
                     DSIEIALGLSGGLVRIETDGEEKVFSGRYACPRCDISFSELEPKVFSFNSPYGACPDC
                     SGLGTRM"
     gene            complement(859..>1603)
                     /locus_tag="P5573_08510"
     CDS             complement(859..>1603)
                     /locus_tag="P5573_08510"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRT28142.1"
                     /translation="NRMIDDLIIEQQAKKAGIVIKEEDVMASISDLLARRNTKLEEFK
                     EALVKEGSSFEEFKEETKKHLMKMRLAQREVRSRIAVSDEEIGEYYSKHRNAYEGKES
                     IRLKQILIAIPNGADAETKKKLRARAEEILKKLKEGEPFELMASRFSKGPEAQAGGDL
                     GFVEKGAMLPSVEEAAFKLKEGELSEVVESPLGFHILKVVDRRGKGTKPLAVVREEIQ
                     NEITNEKMEKKLQEWVQELRKKSFVEVRL"
BASE COUNT          289 a          499 c          401 g          414 t
ORIGIN      
        1 catccgcgtt ccgagaccgc tgcagtccgg gcaggcgccg tagggactgt tgaaagagaa
       61 gaccttcggc tcgagctcgg aaaaactgat gtcgcaccgg gggcaggcat aacggccgct
      121 gaagaccttc tcctcaccgt ccgtttctat cctgacaaga cccccagaga gtccgagggc
      181 tatctcgatc gagtcccgca gacgtttttc gattccttcc ctgcggataa ggcggtcgac
      241 gattacatcg atggagtgac gtttcttcct gtccaggggt atctcgtcgg caaggtcgtg
      301 tacagtgccg tcgacgcgta ccctgacaaa gccgtccttc cgcagtttcc gcagttcctt
      361 ctggaactcc cccttttccc ccgtgacgac cggcgccagg agactgacct tcgttccctc
      421 gggcaaggcc agaacatagt ccatcatcat ttcgacggtc tggggtttga tctccctgcc
      481 gcagcgataa caatgcggga tgccgaccct ggcataaagg aggcggaggt agtcgtagat
      541 ctcggtcacc gtcccgaccg tggaccgggg gttctggctc ggctggcgct gctctatggc
      601 gatggcggga gatagaccct cgatggattc gacttccggc ttgtccatcc ggccgatgaa
      661 ctgccgggca taggtggaga gggattccac gtaccgccgc tgaccttcgg caaagacggt
      721 gtcgaaggcc agggaggact tgcccgaacc gctcacaccg gttatgacca caagcctgtt
      781 tctcgggatg tcgaggtcga ggtgtttgag gttgtgctga gaagcgcccc tgactttaat
      841 aaactccatc ttgtgatctc aaagcctgac ttccacgaat gattttttcc tcaattcctg
      901 tacccactct tgtagtttct tctccatctt ctcattcgtg atttcattct gtatctcctc
      961 gcgtacgact gcgagcggtt tggtcccctt gccacgccgg tccacgacct taaggatatg
     1021 aaaaccgaga ggggattcga caacctcgct cagttcaccc tctttcagct tgaacgccgc
     1081 ctcttccact gacggaagca tggccccctt ttccacaaac cccaggtcac cgcccgcctg
     1141 ggcctccggt cctttcgaga aacggctcgc catcaactcg aaaggctcgc cttccttgag
     1201 tttcttcagt atttcttccg ccctcgccct gagtttcttc ttcgtctcag catcggcgcc
     1261 gttcgggatg gcgatcagga tttgcttcag ccttatcgat tctttccctt cgtaggcatt
     1321 ccggtgcttg ctgtagtatt ccccgatctc ttcgtcacta acggcgatcc tcgacctgac
     1381 ttccctttga gccaacctca tcttcatcag gtgctttttc gtctcctctt tgaattcctc
     1441 gaaagacgac ccttccttca ccaaggcctc tttaaactct tccagcttcg tgttccttcg
     1501 ggccaacaag tcggatatcg aagccatgac gtcttcttcc ttgatgacga tgccggcctt
     1561 tttcgcctgc tgctcgatga tcaggtcgtc gatcatcctg ttg
//
LOCUS       DAOTMX010000340         1599 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_60617_length_1599_cov_2.768782, whole genome shotgun sequence.
ACCESSION   DAOTMX010000340 DAOTMX010000000
VERSION     DAOTMX010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60617_length_1599_cov_2.768782"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1230
                     /locus_tag="P5573_08515"
     CDS             <1..1230
                     /locus_tag="P5573_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HRT28143.1"
                     /translation="VRALHKDRDDEIRIITESVKSKIADLLVGKVSAIKLSDPKKKKI
                     LLKKNDTITAEVLGGIPFSRWRDISLVGDEAVEEKVKTLFDNLDRKVDLVEAFFEEKI
                     ERLKAGDELPPGVIKLVKVYVAIKRKLSAGDKMAGRHGNKGVLSRILPEEDMPFFADG
                     TPVDIILNPLGVPSRMNVGQILETHLGWAAKGLGEKVGALYEKGRDFKAVKGMLKQVY
                     ASPEFARFIDGMTGEELRRFVERLRNGIPMATPVFDGAEEDEIGEMLTQAGLPTTGRV
                     RLYDGRTGEPFDREVTVGMIYMLKLHHLVDNKIHARSIGPYSLVTQQPLGGKAQFGGQ
                     RLGEMEVWALEAYGAAYSLQEFLTVKSDDVAGRTRIYEAIVKGEHNLEPGLPESFNVL
                     VKELQSLALNVKLIENE"
     gene            1350..>1599
                     /locus_tag="P5573_08520"
     CDS             1350..>1599
                     /locus_tag="P5573_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT28144.1"
                     /translation="MRFNAIKISLASPEEILSWSHGEVKKPETINYRTFKPERDGLFC
                     AKIFGPVKDYECLCGRYKRMKHRGVICEKCGVEVTQSKV"
BASE COUNT          397 a          429 c          502 g          271 t
ORIGIN      
        1 gtgagggccc tgcataaaga ccgcgacgat gagatcagga tcatcaccga gagcgtgaag
       61 agtaagatag cggacctcct ggtcgggaaa gtctccgcca tcaagctgtc cgacccgaag
      121 aaaaagaaga ttctcctgaa gaaaaacgac accatcactg cggaggtgct cggcggcata
      181 cccttcagcc ggtggcggga tatctccctc gtgggagacg aggccgtcga agaaaaggtc
      241 aagacccttt tcgacaacct ggaccgtaag gtcgacctcg tcgaggcgtt cttcgaggag
      301 aagatcgagc gtctgaaggc gggagacgaa ctccctccgg gggtcatcaa gctagtcaag
      361 gtttacgtgg cgatcaagag gaaactctcg gcgggagaca agatggccgg gcgccacggc
      421 aacaagggcg ttctgtccag gatcctcccc gaggaagaca tgcccttctt cgccgacggg
      481 actcccgtcg acatcatcct aaaccccctg ggcgttccgt cacgcatgaa cgtgggtcag
      541 atcctggaga cccatttagg ctgggcggcg aaggggctcg gcgagaaggt cggcgccctt
      601 tacgagaaag gacgcgactt caaggcggtc aaagggatgc taaagcaggt gtacgcctcg
      661 cccgaattcg ctcgattcat cgatggcatg accggcgaag aattgcggcg ctttgtcgaa
      721 aggctcagga acggcatccc gatggcgacg ccggtcttcg acggggcgga ggaggacgag
      781 atcggcgaga tgctgactca ggcaggacta ccgacgacgg gccgcgtgcg gctttacgac
      841 ggcaggacgg gtgagccctt cgaccgggag gtaaccgtgg ggatgatcta catgctgaaa
      901 ctccaccacc tggtagacaa caagatccat gcccgctcca tcgggcccta ttcgctcgtt
      961 acacagcagc ccctcggcgg aaaggcgcag ttcggcggtc agagactcgg ggagatggag
     1021 gtctgggcct tggaggctta cggggcggct tactccctcc aggaattcct taccgtcaag
     1081 tcggacgacg ttgccgggag gacccgaatc tacgaagcca tcgtcaaagg ggaacacaac
     1141 ctggagcccg gactgcccga gtctttcaac gtcctggtga aggagctgca gagcctggcg
     1201 ctcaacgtga agttgatcga gaacgagtaa caaacgaaca aaatgagtac cggcggcaac
     1261 ggccgaagat gacgccgaaa ccgctaaaaa cataggagat aaaaagagtg gaagacatat
     1321 tcggtttttt cgaaaaacca aaagacccga tccggttcaa tgccatcaag atctctctgg
     1381 cctcgcccga ggagatactc tcgtggtcgc acggagaggt gaagaaaccg gaaacgataa
     1441 attaccgaac cttcaaacct gagagggacg gcctcttctg cgcgaagatt ttcgggccgg
     1501 tgaaggatta cgaatgcctg tgcggccgct acaagcggat gaagcaccgt ggggtcatct
     1561 gcgagaagtg cggtgtggag gtcactcagt cgaaggtgc
//
LOCUS       DAOTMX010000341         1591 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_61083_length_1591_cov_2.884115, whole genome shotgun sequence.
ACCESSION   DAOTMX010000341 DAOTMX010000000
VERSION     DAOTMX010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61083_length_1591_cov_2.884115"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="P5573_08525"
     CDS             <1..623
                     /locus_tag="P5573_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRT28145.1"
                     /translation="MVLTDSKALYDRADWYHDHGHDHNPAVSRAMEGRTILGFNYRMN
                     ELQGAVGLAQLRKLDHIVAEQKKNKAAIKETLTAVKGVGFRELADSDGDSATFLAFNM
                     PEEARAKRFQQALGAEGVDTVCYKDNLWHYLPNWEHFLAKSTANSKRYPFADPSYKGK
                     VTYDRSGFPKAEDLLGRTLVMGINVKMPTERLDAVRRGIEKAAKAL"
     gene            660..>1591
                     /gene="rfaD"
                     /locus_tag="P5573_08530"
     CDS             660..>1591
                     /gene="rfaD"
                     /locus_tag="P5573_08530"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR02197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HRT28146.1"
                     /translation="MIIVTGGAGFIGSVFVWKLNEEGVDDILIVDELGESEKWKNLVK
                     RRFSDYLHKESFLEMVLADKVPFQVKTVVHLGANSSTTERDAEHLMRNNYLYSRALAV
                     WAVRNDVRFVYASSAATYGDGAQGFSDEDAVTRTLRPINMYGYSKHLFDLGILKNELG
                     EKVAGVKFFNVFGPNEYHKGDMRSVVHKAFGEIRETGKVRLFKSYKPDYPDGGQMRDF
                     IYVKDCVDVLWWLCRNPGVGGIFNLGTGVARTWNDLARAVFSALDMEPQIEYVEMPEG
                     LRDQYQYFTEARMEKLRAAGYRAEFRSLEEGVRD"
BASE COUNT          358 a          441 c          515 g          277 t
ORIGIN      
        1 ggatggttct gacggacagt aaggcgctgt acgaccgcgc cgactggtac cacgaccacg
       61 gccacgacca caaccccgcg gtcagccggg cgatggaggg gcggaccata ctgggtttca
      121 actaccggat gaacgagttg cagggggccg tcgggttggc ccaactgaga aagctggatc
      181 acatcgtcgc cgagcagaag aagaacaagg cagcgatcaa ggaaaccctc acggcggtga
      241 agggtgtcgg tttccgggag ctcgccgact ctgacggcga ttcggccacg ttcttggcct
      301 tcaacatgcc cgaggaggcg agggcgaaga gattccagca ggccctcggt gcggagggcg
      361 tcgacacggt ctgttacaaa gataacctat ggcattacct ccccaactgg gagcacttcc
      421 tggcgaaatc tacagcgaat tccaagaggt accccttcgc tgacccatcc tataagggga
      481 aggtgaccta cgacaggagc ggcttcccta aggcggaaga cctcttgggc cggacgctgg
      541 tcatggggat aaacgtaaag atgccgacag agagactcga cgctgtcaga cgggggatcg
      601 aaaaggcggc gaaggcgctt tgatggccgt cggcccttag atagaggaaa acgaagagaa
      661 tgatcatcgt aacaggcggt gcgggtttca tcgggagcgt tttcgtctgg aagctcaatg
      721 aagagggggt cgacgacatc ctgatcgtcg atgaactggg cgaatcggag aagtggaaaa
      781 acctcgtcaa gcggcgtttc agtgattacc tccacaaaga atcctttctc gagatggtgc
      841 tagccgacaa ggttcctttt caggtgaaga ccgtagtcca cctcggcgcc aactcctcga
      901 cgacggagcg cgacgccgag cacctgatgc gcaacaacta cctttacagc cgcgccctcg
      961 ccgtctgggc ggtgaggaac gacgtccgtt tcgtctatgc cagcagcgcg gcgacatacg
     1021 gcgacggcgc gcaggggttt tccgacgaag acgccgtgac ccgtacgctc cggccgatca
     1081 acatgtacgg ctactccaag cacctcttcg acctggggat cctgaagaac gagttggggg
     1141 aaaaggtggc gggggtcaag ttcttcaatg tcttcggccc caatgagtac cacaagggtg
     1201 acatgaggag cgtcgttcac aaggccttcg gcgagatccg ggaaacgggg aaggtaagac
     1261 tcttcaagtc ctacaagccg gattaccccg acggggggca gatgcgggac ttcatctacg
     1321 tcaaggactg cgtggacgtc ctctggtggt tgtgccggaa ccccggcgtc ggggggatct
     1381 tcaacctcgg cacaggtgta gcgcgcacgt ggaacgacct ggcgcgggcg gttttctccg
     1441 ccctcgacat ggagccgcag atcgagtatg tcgagatgcc cgaaggactg cgcgaccagt
     1501 accagtattt caccgaggcc cggatggaga agctccgcgc cgccggctac agggcggagt
     1561 tccggtccct cgaagagggc gtccgggatt a
//
LOCUS       DAOTMX010000342         1564 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_62639_length_1564_cov_3.445991, whole genome shotgun sequence.
ACCESSION   DAOTMX010000342 DAOTMX010000000
VERSION     DAOTMX010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62639_length_1564_cov_3.445991"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="P5573_08535"
     CDS             <1..310
                     /locus_tag="P5573_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT28147.1"
                     /translation="GYAVGRGFTHREEVVQPHLFKKLRNARDSGVELITTVCPGCNVA
                     LDREQPNLRERYGEDFRIPVVDLAQLIALALGVPAAKLGFEANTIPLDDVLAKLGISE
                     "
     gene            324..1370
                     /locus_tag="P5573_08540"
     CDS             324..1370
                     /locus_tag="P5573_08540"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRT28148.1"
                     /translation="MNEAMTGKEHTALVIGGGPAGIEAALKIARGGYRAVLVEKESEP
                     GGIPLKLFSSFPRWENPSDRIGWMVNDLKKNKQIEISLETTVESAAGTDNGFNVTLKN
                     TATGDARTLDVQAVVLATGFRLMDLSAYGEYGHRIYPGVLNSLEFEERLREWSRGLGK
                     APQAVALIQCVGSRDRSKGYPYCSKICCMYSAKQAGVVKDLFPEAKVYVFYIDCRAAG
                     KGYEEFLREVIEKKHVRYVRGRPAKVLPEGGRLLVRAEDTLIGIPVEFAADVVVLAAA
                     VVPHPETAALAKMFGASIDEYGFIDHDPRSHAKCADRVFFAGGCGFAVDTESALSQGA
                     AAAAEVITLFNDNG"
BASE COUNT          351 a          467 c          466 g          280 t
ORIGIN      
        1 cggatatgcc gtgggaaggg gcttcacgca ccgggaggag gttgtgcaac cccatctctt
       61 caaaaaactc aggaacgcgc gcgactccgg agtggaattg atcacgaccg tttgtccggg
      121 ctgtaacgtg gccctcgaca gggagcagcc gaacctgcgt gagcgttacg gcgaagactt
      181 ccggattccg gtagtcgacc tcgcccagct catcgccctg gcgctcggcg tcccggcggc
      241 gaagctcggg ttcgaggcca acactattcc cctcgatgac gtcctggcaa aactcggcat
      301 ctctgaatga aggcgatgga aagatgaacg aagctatgac gggaaaagag catacggcgc
      361 tggtaattgg cggaggtccc gccggcatcg aagcggcttt gaaaatcgcc cgcggcggct
      421 accgcgccgt tctcgtcgag aaagaaagcg aaccgggcgg gataccgtta aaactcttca
      481 gcagcttccc tcgctgggaa aacccctccg acaggatcgg ctggatggta aatgatctca
      541 aaaaaaacaa gcagatagag atttccctgg agacaaccgt cgagtctgcc gcgggcacgg
      601 acaacggctt caacgtaaca ctgaagaaca ccgcaaccgg tgacgcccgg acactcgacg
      661 tccaggccgt cgtcctggcg acggggttca gactgatgga cctctcggcc tacggagaat
      721 acggccacag gatatacccg ggtgtcttga actcgctgga gttcgaagaa agactcaggg
      781 aatggagccg cggcctggga aaggccccgc aggccgtcgc cctcatccag tgcgtcgggt
      841 cccgcgaccg ctccaaaggc tacccttact gctcaaagat ctgctgcatg tactcggcca
      901 agcaggccgg cgtcgtgaaa gacctcttcc ccgaggcgaa ggtctatgtc ttttacatag
      961 actgccgcgc cgccgggaag ggctacgaag aattcctccg tgaagtcatc gagaaaaaac
     1021 atgtacgtta cgtccgggga cgtccggcca aggtcctgcc cgagggcggc aggctactcg
     1081 tcagggccga ggatacactg ataggcatcc ctgtggagtt cgccgccgat gtcgtcgtcc
     1141 ttgccgctgc ggtcgttccc cacccggaga ctgcggccct cgcaaagatg ttcggcgcct
     1201 ccatagacga atacggtttc atcgaccacg acccgcggtc tcacgcaaaa tgcgcggacc
     1261 gggtcttctt tgccggcggc tgcggcttcg cagtggacac cgaaagcgcc ctgagccaag
     1321 gcgccgcggc ggccgccgag gtgatcaccc tttttaacga taacggatag tcaaaccttt
     1381 ggacgtcgat gatttaggaa agcaaggtat ggacaccgat ttttacatcc ccatgcggct
     1441 cgacgaggta gaaagcccgc tccagaccga gatccagaag acgctcggca tcgatatcga
     1501 gacctgcatg gagtgtggaa aatgctcggg tggctgttcc aacgcccaca tcttcgattt
     1561 cacg
//
LOCUS       DAOTMX010000343         1538 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophales bacterium isolate AMR_MDS_2910
            NODE_64232_length_1538_cov_3.164531, whole genome shotgun sequence.
ACCESSION   DAOTMX010000343 DAOTMX010000000
VERSION     DAOTMX010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867934
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophales bacterium (wastewater metagenome)
  ORGANISM  Syntrophales bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:43:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,711
            CDSs (total)                      :: 1,685
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Syntrophales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64232_length_1538_cov_3.164531"
                     /isolate="AMR_MDS_2910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2928017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /gene="aroC"
                     /locus_tag="P5573_08545"
     CDS             <1..542
                     /gene="aroC"
                     /locus_tag="P5573_08545"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HRT28149.1"
                     /translation="PLLCPDRKAAARMEKLLEKARNEGDTLGGIVEIVVRGCPPGLGE
                     PVFDKMDADLAKALMGIGTVKGVEIGSGFEAARMTGSRHNDPLTPGGFATNHAGGVLG
                     GITSGEDIVVRVACKAIPSIGKAQKTVDLNGKPVTLSIKGRHDVCVIPRIIPVCEAMV
                     SIVLADHLLRQRANRGVFS"
     gene            539..1294
                     /locus_tag="P5573_08550"
     CDS             539..1294
                     /locus_tag="P5573_08550"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HRT28150.1"
                     /translation="MNNIEVGIIGGTGGIGEWFARFFREEGLTVHVSGRTTGMPAVDM
                     ARRCDIVIVGVPISATCDAIRLVGPMMNRKSLLMDLTSLKAEPVKAMLESSTSEVIGL
                     HPLFGPDVPSLEGQNIVLCPARGTTWLPRVKELFLSRGAQLVEASPERHDELMAFVQV
                     LTHLNTITMGLALRDADIPQGELENFSTPIFRARLASIEKIFLKNPRLYSEVIASNPY
                     TPKIMALYGDALRRLEETVGTGKAEELKRLIEG"
     gene            complement(1319..>1538)
                     /locus_tag="P5573_08555"
     CDS             complement(1319..>1538)
                     /locus_tag="P5573_08555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT28151.1"
                     /translation="GLSSLSGNHMVLSSTVIRLLREKGAGDIPVIIGGKIPPDDVASL
                     KEVGVLEVFTPGTPIETVISFIADIEKK"
BASE COUNT          345 a          451 c          451 g          291 t
ORIGIN      
        1 accccctgct ctgccccgac aggaaggccg cggcgaggat ggaaaagctg ctggaaaagg
       61 cgcgcaatga aggagacacc ctgggaggga tcgtagaaat cgtggtgaga gggtgcccac
      121 ccggactcgg agagcccgtg ttcgacaaga tggacgcaga cctcgcaaag gccctgatgg
      181 ggataggaac ggtcaagggc gtggagatcg gctccggctt cgaagcggca aggatgactg
      241 ggtcgaggca caacgacccc ctgacaccgg gagggttcgc cacaaatcat gccggcggtg
      301 ttctcggcgg tataacgagt ggtgaagaca tcgtggtccg ggtcgcctgc aaagccatcc
      361 cgtcgatcgg gaaggcgcaa aaaacggtcg accttaacgg caagccggtc accctctcca
      421 tcaaggggcg tcacgacgta tgcgtcatcc cacggatcat ccccgtctgc gaggcaatgg
      481 taagcatcgt ccttgcagac cacctgctga ggcaaagggc gaataggggg gttttctctt
      541 gaacaacatc gaggtcggga tcatcggcgg tacaggcggc atcggagaat ggttcgcccg
      601 cttttttagg gaagaaggac tgacggtcca cgtctccggc cgaacaactg gcatgcccgc
      661 cgtcgatatg gcccggaggt gcgacatcgt gatcgtcggc gtccccatca gcgccacctg
      721 cgatgcgatc cgcctcgtcg ggcccatgat gaacaggaag tccctcttga tggacctcac
      781 gtctctcaag gcggagccag tgaaggcgat gctcgaatcc tccacctcag aggtcatcgg
      841 ccttcatccc cttttcggcc ccgatgtccc ctcactcgag ggacagaaca tcgtcctctg
      901 tcctgcccga ggaacgacgt ggcttccccg ggtgaaggaa ctttttctga gccgcggcgc
      961 ccaactcgtc gaagcgtcgc cggaaaggca cgacgaattg atggcctttg tccaggtact
     1021 cactcatctc aacaccatca ccatgggcct cgccctcagg gatgccgaca taccgcaggg
     1081 cgaacttgaa aatttttcga cgcccatctt ccgggcaaga cttgccagca tcgaaaagat
     1141 attcctgaaa aacccacgcc tctacagcga ggtcatcgct tcgaatccgt acaccccaaa
     1201 aatcatggcc ctgtacggag atgcgctccg ccgactggaa gagacagtcg ggacgggaaa
     1261 ggcagaggaa ttgaaaaggc ttatcgaagg gtgaactccc cttcttacat ttccctgttc
     1321 actttttttc gatgtcggcg ataaaagaaa tgaccgtctc gatgggcgtc cccggagtga
     1381 agacctccag gacacctact tccttgaggg atgcgacatc gtccggaggt atcttgccgc
     1441 cgatgatcac cggtatatct ccggcacctt tttcgcggag gagtcttatc accgtggacg
     1501 ataggaccat gtggtttccg gataacgagc tgaggccg
//
